LOCUS       DAOQWT010000001        19136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_3248_length_19136_cov_6.245165, whole genome shotgun sequence.
ACCESSION   DAOQWT010000001 DAOQWT010000000
VERSION     DAOQWT010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 19136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19136
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3248_length_19136_cov_6.245165"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..591
                     /locus_tag="PKJ95_00005"
     CDS             313..591
                     /locus_tag="PKJ95_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64656.1"
                     /translation="MLTGVFAKVLFTLYDETQGPIITEKTLNMSAEDFFVSVNFLVDN
                     GYVKESAVKKHPSIDLIHVERIGALTLKGIEEGKYIAINGITPDIMGG"
     gene            complement(1203..1472)
                     /locus_tag="PKJ95_00010"
     CDS             complement(1203..1472)
                     /locus_tag="PKJ95_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64657.1"
                     /translation="MISQINGRVHTKNILPKHRTQSHSRPVLGHITGIHTVVIIIPIF
                     TNGRKCRLNAVFSSIGLFAGMGKTGDLLFHLAGAQGEGCHGTHPR"
     gene            complement(1601..2308)
                     /locus_tag="PKJ95_00015"
     CDS             complement(1601..2308)
                     /locus_tag="PKJ95_00015"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(p)ppGpp synthetase"
                     /protein_id="HNR64658.1"
                     /translation="MNEDIRDKILEIFLSEKFSIDQFHKRLINYFADNPKLSKTAFPI
                     IHSIKFRIKDINHLKEKLDRKDNSDNPITENNFFDRITDLAGIRILHLYPSHFEQIHN
                     EIMSQTHAGEWFLFEPPIAYTWDPVLEKIFKGLEINTEIKESYYTSVHYILMPQKISK
                     IKCEVQVRTLFEEIWGEIDHSMSYPKKSDSVSCIEQLKVLSSLSATGSRLLESILKSK
                     NEYEFLSINIRETQSLL"
     gene            complement(2305..3402)
                     /locus_tag="PKJ95_00020"
     CDS             complement(2305..3402)
                     /locus_tag="PKJ95_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR64659.1"
                     /translation="MKIISIFNNKGGVGKTTLTYHLAYALSEIGKKVLLIDLDPQSNL
                     TLYGLSIDDIHDIWEEENPFIEEGFESTIRKIGEKEFNRILHKPKTIHFLLKPTEEGI
                     NDFDILPPLIDINNNLKLLPGRLTLHMYENKISVRWNDSYSGDPLSIKTINKIRDISK
                     KYSDKNNFDFVIIDTSPNLGSLNKVIISTVDGFIIPALPDMFSLYGIRNIGNSLKSWK
                     ENFDTIYKLISEEKRKQFPENFVRFLGYTIYNARKYSRGKEWDLAQAHYNFARQIPST
                     IKQYINEDIRKNLSEEMINSPIGGICVMHTHNTLPSMAQKYRNPMWKIPTIELDPEDS
                     APVTGNRIAYLATQENYKKFAEDLLKRINTL"
     gene            complement(3997..4410)
                     /locus_tag="PKJ95_00025"
     CDS             complement(3997..4410)
                     /locus_tag="PKJ95_00025"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNR64660.1"
                     /translation="MLKEEVDKLIEFLKSSDRIKDRKERLEAIEKLGNSGYIDKVPFL
                     CELLLNDYWDIQCKAAEAVGKLGDKKAIEALRKSLKHRQWHVREATALALGTLKAEEA
                     IEELRKQLNDKSYGARESAKTALSMITGEIYKKGD"
     gene            complement(4611..6638)
                     /gene="brxL"
                     /locus_tag="PKJ95_00030"
     CDS             complement(4611..6638)
                     /gene="brxL"
                     /locus_tag="PKJ95_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HNR64661.1"
                     /translation="MEDINKKLLSLFPGKVVRKDLTKLIKEGANVPTYVLEYLLGMYC
                     SSDVEETVKWGLEKVKGILSDNYVRTDEAEKIKSHIREKGRYKVIDKVTVHLNEKKDR
                     YEAVLTNLGLKEIVTSPDYIKKFEKLLAGGIWCILTIEYFYDESAKDSPFVLRELKPI
                     QMPNMEMNELFTGRKSFNTKEWIDIILRSVGMEPANLDEKSRWHLIARLIPFVENNYN
                     MCELGPRGTGKSYVYKEMSPNSILVSGGQTTVANLFYNMATRRVGLVGLWDIVAFDEV
                     AGINFKDKGGVQIMKDYMASGSFARGKDPVEANAAMVFIGNINEPIEHLLKTSHLLSP
                     FPDEMIDTAFFDRFHIYLPGWEVPKMRPEFFTDQFGFITDYLAEWFREMRKKNFADAI
                     NKYYKLGNNLNQRDVIAVKKTVSGLLKILYPHGEFTKEELKPCLEYALTGRRRIKEQL
                     KKIGGMEFYDVHFSYIDPEKEEEKFITLPEVGGRNLIPEGRQNPGIVYVIAKSIETGH
                     KGLYKLEIQTMSGSGKFSHTGFGSKKITGDELKEGENYFKANLTRISGESKYTETDFH
                     MKAVDMNGIGEAKEIELAVFLSLISGITRKPLQPQMVVIGSMSIGGTIIKTDSLADAL
                     QIAVDSGAKKVLIPVSNMVELVTVPPELFTKLQLIHYNDPADAAFKALGVE"
     gene            complement(6644..9145)
                     /gene="pglZ"
                     /locus_tag="PKJ95_00035"
     CDS             complement(6644..9145)
                     /gene="pglZ"
                     /locus_tag="PKJ95_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HNR64662.1"
                     /translation="MDKIKERLNQLFETHRLIFWYDEEGDMRQIYQDIDLSDIEKIEI
                     KNNEFYIKYKVICQEPDKKFLIYAPYSEPPAETNWLIDLLIANISFSADHPSMIIQDL
                     KLGLEMKSFIKKHISFFKSQKRIEKLKSLISENDEQKRLALKMISVITGTIPQIEEIL
                     YSLFTEMDKDSDNSWKNIEKYGLNEYFWEMIDETFHYKTDNPTIKDLLIKIILFHFYS
                     SLKAEVKESYTKLSQDSIIFLNRWMDSSKNKESLISLVVKIEKESGLKNKFLTYDYRD
                     LKDCDILESVEQKIIQDIKNKLLKEIINIDEAISVIQKRKLSLWYKEYEKIYEALNMA
                     LEIYKIKNMAVDFNSFDEGLSVYSKSLYKFDTLYRKYIYYSKNQGETTLLKELDSTIE
                     NIYSCVFLPSLNDKWQKYVDSCKEWKSKLIPGQDSFFDTCVNPYLKKDQKIFVIISDA
                     FRYETGAELYERILKEDKLEGDLSFMLSMLPSYTALGMAALLPHKELSCDMEKDRFLI
                     DGQSIQSIDDRQKVLENFNPSCTAIKADNFLSFTRDTGREFAKKYNVIYIYDDTIDAT
                     GDDFKTELNVFDAVEQTFERMEKIIKHIVNFNGTNIIITSDHGFLYQHREPEDIDFKK
                     ITDTGGTILKKNRRFLIGKDLIEISPTKKFTSSELNISGDYDFLIAKSIKRLKTQGGG
                     SRFVHGGSSLQEIVVPVIKVKCKRESTTKKVEVDIIQTQSSITSNQFPVNFVQKEPLS
                     DKVLPRKLKCGFFTKDGILISDENTLLFESKEDDMRNREQKCIFRFKSDSSDYNGQPV
                     ELKLKELIENTNEYAEYKKVLYDMHIAFGNEFDEW"
     gene            complement(9157..9534)
                     /locus_tag="PKJ95_00040"
     CDS             complement(9157..9534)
                     /locus_tag="PKJ95_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64663.1"
                     /translation="MDSCEKESIYEKVMREHRNKDKVLPIPLHELNTIEKIIDVLKHL
                     PLTIEQRDVYEEKLKILRDEESILETARMKAMEKGIMEGRKKAEVKIARKSLEMGLSE
                     EIIRDVTGLTEEEIIKLKSEIHS"
     gene            complement(9599..10432)
                     /locus_tag="PKJ95_00045"
     CDS             complement(9599..10432)
                     /locus_tag="PKJ95_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNR64664.1"
                     /translation="MCKINPRVDFAFKLIFGNEQNKDILISLLNSILEDYQTRPIASI
                     EILNPFGSKEHERDKLTILDIRAKDDKGEFYNIEMQIADQYYYNKRATYYWARLYSSQ
                     LGEGRAYNNLKRTVSINILNFNRLEEKDYHSIYRIKNLKTGKDLHDQLEIHFIELEKF
                     TKELPDLKKTLDRWIYFLKYAEKFTELNLPVALKEIDTIEKATRILDKISLTDEEREI
                     YESRLKALRDEDGALETARIQGKIEVAKNMLREGLSVELICKYVGLPSEEINMLIDKW
                     K"
     gene            complement(10438..13797)
                     /gene="pglX"
                     /locus_tag="PKJ95_00050"
     CDS             complement(10438..13797)
                     /gene="pglX"
                     /locus_tag="PKJ95_00050"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF033452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HNR64665.1"
                     /translation="MDKNNLKIFARQLRNRLREMTEVKINYYLTGEDIEIKYREKQIG
                     DLKKEIDSIGLDGVIEKVSYIWFNRFVALRFMELKGYTKSRILSPLPGRTVPEILEQA
                     KGGLIDEELRLDRNKINDLLDGKIRSRDPEEEVYRMLITGVCNYYHKIMPFMFEWIDD
                     YTELLIPENLLKAGSVRDEIVKNISEDDCEDVEIIGWLYQYYISEKKDEVINAKKRYK
                     PDEIPAATQLFTPKWIVKYMIDNTLGQLWLEARPDSCLKEFMEFYIEPADKDKIPPRK
                     INSPEEITIFDPACGSGHILVYAFDLLIKIYEEEGYSKSEIPSLILEKNLYGIDIDDR
                     AASLASFALMMKARGYYRRFFDKGIVPNVMAFMDSDEYEIFKNAKVLGSLIRVSEEDI
                     KRLEEDRGSLFEVVQENLRKQARFLWQKYDCVVTNPPYMNLKYIERSLNEFVKKHYPE
                     TKFDIFACFILKCCSMGKNDSILGFMTPFVWMFIYFYEWLRVFLIENKVISNLIQLEY
                     SGFAEATVPICTFTLRNNNLDIKGDYIKLSEFTGAENQPIKTLNAIKKSDIYYRFKAN
                     QKDFKKIPGNPIAYWASDKMKEIFEKANKIRELFDVKTGIQTGNNDKFVRFWYEVFFN
                     KIGIGFSHYSDILIEKKKWYPYNKGGEFRKWYGNNFHIINMENNGEEIKKETNYRLRD
                     PNFYFKETITWTDISSSNFGVRFSPEGFLFDVAGSSLFPKEDKIKYTTAFLCSKLTCD
                     FLKILNPTLNYQVGNISSLPIIFTENKPLKIIIDTLSEQNISISRTDWNSRETSWDFK
                     SNSLILCKNEKIEEAYKIWKSSAESQFCTLHRNEEELNKIFIEIYGLQDELTPEVELK
                     DITILRDETFINDRGELEFKPDEVMKQFISYSVGCIFGRYSPDKEGLILANQGETVEN
                     YIKIINQAPSFNPDEDNILPILSDEYFENDIVSLFNKFLKITFGEGTFHENLAFIEEK
                     LGKSIRAYFEKDFYSDHVKRYRKRPIYWMFQSPKKYFQALIYMHRYTPDLCSKVLNEY
                     LREYASKQEYRKVQIKKDIDSDTLSGKEKTKKDKEIKEINKILKDLAEYDKKLDHMAK
                     QYIPIDLDDGVRVNYCKFKDVLATIAGLEKE"
     gene            complement(13866..17360)
                     /gene="brxC"
                     /locus_tag="PKJ95_00055"
     CDS             complement(13866..17360)
                     /gene="brxC"
                     /locus_tag="PKJ95_00055"
                     /inference="COORDINATES: protein motif:HMM:NF033441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HNR64666.1"
                     /translation="MLIRNIFEQDISREIEGVIKADDYSHITQEVKEYVITNEVEEKL
                     DDFFRVYTKDISSKTSRDTGVWVSGFFGSGKSHLLKILSYLLENREIDGKKTVDIFLN
                     KIKNGDFELAANIKSASSIPVKTILFNIDQKADQINNQKEPVLSVIMKVFNEFSGYYH
                     RSLNIAEFERNLDRLNLYEKFKENYRKVSEGETWEEDREVIDLKITEFIQALSMTKNI
                     SQEDGREVFRTVTENQKNLSIEDFAYIIKDYIDKQPDKFRLIFLIDEVGQYISNDSHL
                     MLNLQTIAETFGTICEGKAWLIVSSQHNLEAIVGDITKSQSQDFSKIMGRFRTKLNLT
                     SKNVDEVIQKRLLAKTPESTEKLDKFYHDNQTRLKSLHFKDAGKNLPKVKDKESFIAD
                     YPFLPYQYELMQDAIIQLGRHNALLGKHQSFGERSMLEVYQLSAMELSSKEISSLAPF
                     DLFFSSIKGVLKTEAVLGLNNAEKNDLLKPFDLRVLKSLFLVRYIDHFKTNVQHITTL
                     LIDSVDTDEIALEKNIKNSLEKLEREIYIQNIGGIYHYLTDEEKDIQEEIKEMDTEDT
                     EISSSLAEIVFDEIIGDTKFDYKPIKQSYSFTKKMDDRHYSKEHFLGIHFITPLYIEE
                     KNLTDMELKTKSMGCNDIIVSLPELENTRNQLRNQLILIIQTKKYIKKSSTSMSDRKR
                     LLFEQTASQNSERRSKLIEQIKDLINDARFYLNGEEIKISNMTGVKPRLNEAFQKAVE
                     VTFPKLKMLTKVYKEDDIKEILFSGSDLFGSLTEGEDEVKVFIERKRPYGRVTVKHII
                     ENFTGNGYGWYQPAILSQIAGLYSKKKLMINYNSVELQDYKEIYKKLLNSKEFDSITV
                     EIQSKVDEGKIAKAKEFYQELFNISLTVNDQKDVYEEFRNKLKEEISKLEILIGKSSE
                     YHFLTELIELVDELKELARTGMNDFYDKIEDKMGHILDINEDVIEPVTGFMNSPQKQI
                     YDDIKKFISSQQVNLEKVSDEQKIRSLKMVMEDRKPYRTGYMQKSKNLLEDLKSSLTP
                     LIDEAKKRIIEKARSYILKLEDYEDYKQISQDMQKEILKPFEDFIKKGINEELIPVIN
                     DKSGKLDELYEKQLKKIAAEFAKKSNVIPDEIVNIKKIKLSYKKTTLETADDVENYVK
                     DLREKLLDEVNNKRKIYL"
     gene            complement(17388..17960)
                     /locus_tag="PKJ95_00060"
     CDS             complement(17388..17960)
                     /locus_tag="PKJ95_00060"
                     /inference="COORDINATES: protein motif:HMM:NF020329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HNR64667.1"
                     /translation="MNKKITVARFKDLYEKFKTQDFLTSRCTGNEIPFYIFAYEAQLE
                     YFVSDEIKMLEKRLLKDGISILNINLYSLCIEILKERKLLDLFIKKEPEFTKDKLMEN
                     LKGALDAETKIGPAIEKKVKDSSKNLLFITGTGLVYPYIRVHNLLNNLQSRVKDIPVV
                     IFYPGIYTGQSLSLFGKIKAENYYRAFNLA"
     gene            complement(17953..18585)
                     /locus_tag="PKJ95_00065"
     CDS             complement(17953..18585)
                     /locus_tag="PKJ95_00065"
                     /inference="COORDINATES: protein motif:HMM:NF020425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HNR64668.1"
                     /translation="MKYNLSFIGASLFPNESIKISKLYLEIKNWEEVQKTVIEENILQ
                     KNTLSTTKREYRELKKRLENLSFPLLEYMVNATQDMAKLTLIYAICKASHIIYDFIVE
                     VIREKYLLFDYTLLDMDYEKFIRTKEEIELLSPITDKTAKKTKNMILKILEQGSLLNS
                     VKERQIQRPYIDEQLLKLIYRHDKHADKYFKIFLLSDTDITNYAERFSNE"
BASE COUNT         5435 a         3350 c         2952 g         7399 t
ORIGIN      
        1 gcctttaaaa gaatattcta ttatttacta ctgtcacaaa aggtaaggtt attgtgacat
       61 aataaaatag tgtcacaaaa gtcgatttgg tattttgtga tacatatgat gtaggggatt
      121 ttcataaaat ttgatagtga aaatctctta tggctgtttt ttacattaat agttgccgta
      181 ccccaataaa aaaacaacta ttttaaaata acaataatat tcattctgaa aaatataata
      241 atatccatag aaaattcatt atagttatta tataattatt agttgaagat taaaaaaaca
      301 aggagtaagt atatgttaac aggagtattt gcaaaagtat tatttacttt atatgacgaa
      361 acacaaggac ctataattac cgaaaaaaca cttaatatgt cagcagaaga tttttttgta
      421 tctgtgaatt ttctcgtaga taatggctat gtaaaagagt ctgctgtaaa aaaacatcca
      481 tctattgatt tgattcatgt cgaaaggata ggagcactaa ccctaaaagg tatagaagaa
      541 ggtaaatata tagccattaa tggaataacg ccggacataa tgggaggttg atagcaaatg
      601 gacaaggggt gcattcccaa tttatagatc attcttgggt attttaggag accattaaaa
      661 actgtctgta gattttcatt aaaaattgac cccctgcgaa taatatttcc acatagatcc
      721 tacttgagct atttttgtat atttaatcaa aatcgatatg atataataat catagtttta
      781 tatacgatat taagatacag tatttccggg taaaaatagg ttgttttcac ctgtatcata
      841 aacgaaggtt tgtgaggcat tttttttatt gctttgtatg tatcactaac gataaattag
      901 tgatacataa taggggaggt agttaggaaa atggctaaaa taaaggaaaa tattgataat
      961 ggtatagatg ataaattgaa gagatttgat gtaataatgg actattctct tttagataaa
     1021 attaagtttt aatatattaa aatagtcagg gccttttaat tgaaggaaat ttacctgtaa
     1081 aattcaggag taaataacct ttgaaaaaaa atttcttata cgttgcccct ggtgacacgt
     1141 ttgctcttac tgggcctata tgggttcagt tgttcaatat ccggtagaaa tttttcttcc
     1201 ggctaccttg gatgagtacc atggcatcct tcgccctggg ctcctgccag atgaaaaaga
     1261 agatctcctg ttttgcccat ccctgcgaaa aggccaatag atgaaaatac cgcatttaaa
     1321 cggcatttcc tgccattcgt gaaaataggt attattatta ctacagtatg aatgccggta
     1381 atatgcccta aaaccggtct ggaatggctc tgagtgcggt gttttggaag tatatttttt
     1441 gtgtgaaccc gcccgttaat ttgggagatc ataggggttt tgggggcgtt cgggggtggc
     1501 aacagtaggg gtaaaaaagc ttgccttatc gtaaaaaatt acacgatttt tgcctggtac
     1561 ccttcttacg aaaaaacgct aacagcgtaa attcgtaaaa ttataggagt gattgtgtct
     1621 cacgtatatt aatagagagg aattcatatt catttttact cttaagaata gattctaata
     1681 atcttgaccc tgttgctgat aaacttgata atacttttaa ttgttctata caactaactg
     1741 aatcactttt ctttggataa gacatgctgt gatcaatttc tccccaaatc tcctcaaata
     1801 atgttcgaac ttgtacctca catttaattt tactaatctt ttgtggcatc aaaatataat
     1861 gtacacttgt ataatatgat tcttttattt ctgtatttat ttctaatcct ttaaatatct
     1921 tctctaatac tggatcccag gtatacgcta taggaggttc aaataaaaac cactctcctg
     1981 catgggtttg tgacattatc tcattatgta tttgttcgaa atgtgatgga tataaatgaa
     2041 gaatacgtat acctgctaaa tccgttattc tatcaaaaaa attattttcc gttattgggt
     2101 tatcactatt gtcttttcta tccaattttt cttttaagtg attaatatcc tttattctaa
     2161 attttattga atgaataatg gggaaagctg ttttggacaa ttttggatta tcagcaaagt
     2221 aattgattaa tcttttatgg aattgatcaa tcgagaattt ttcgcttaaa aatatttcta
     2281 atatcttatc cctaatatct tcattcataa tgtgtttatt cttttcaaaa gatcttctgc
     2341 aaattttttg tagttttcct gtgtggcaag atatgcaatc ctattacccg ttacaggagc
     2401 actatcctca gggtctaatt caatagtagg tattttccac atagggtttc tatatttttg
     2461 agccattgaa ggtagtgtat tatgtgtatg cattacacat atccctccaa taggactgtt
     2521 aatcatctct tctgataaat ttttacgaat atcttcgtta atatactgtt taatagtgct
     2581 aggaatctgt cgtgcaaagt tataatgagc ttgagctaaa tcccattctt tacctctaga
     2641 atacttcctt gcattgtaaa ttgtataacc taaaaatctt acaaaattct caggaaattg
     2701 ttttcttttt tcttcagaaa taagtttata aatagtatca aaattttctt tccaagattt
     2761 taatgaatta ccaatatttc ttatgccata taatgaaaac atatctggca atgcgggaat
     2821 tataaatcca tctacagttg aaattataac tttatttaat gaacctaaat ttggtgaggt
     2881 atcaataata acaaaatcaa aattattttt gtctgaatat ttctttgata tgtcccttat
     2941 tttatttatt gtttttatag ataaaggatc accactgtaa gagtcattcc acctcacgga
     3001 aattttgttt tcatacatat gtaatgttag tcttcctggt aataacttta gattattatt
     3061 tatatcgata agtggtggta aaatatcaaa atcatttatt ccttcttctg ttggtttcaa
     3121 taaaaaatga atagtcttcg gtttgtgtaa aattctattg aattctttct caccaatttt
     3181 ccttattgta gattcaaatc cctcttctat aaatggattt tcttcttccc aaatatcgtg
     3241 aatatcgtct attgataaac cataaagagt aaggtttgat tgaggatcta agtctattaa
     3301 taatactttc ttaccaattt ccgataatgc atacgctaag tgatatgtta aggttgtctt
     3361 ccctacacca cctttattat taaagattga aataattttc atatatactc ctgacgttta
     3421 attttaaact attattatac ataattcctt attaagggta ccaggcagat taatataaaa
     3481 atgtgcggtt tggtgtttaa acccttgcca caattgacgt tacgatacat aatattttca
     3541 ttggttttcc ctatagaatt ttcttcctga ataatttact gcaaaaaacg atcgtttctt
     3601 tcagttacat ttttctattt tatctttgtt gattttactg ggtttgtaaa ctgcaaaaac
     3661 tcatgcaaaa atcaaactgc agaaagtcaa attcaagtta agtttctgca taaaataggc
     3721 ttaccgtaca tttttacatc aaactgccca gaaccatggt aagggtaaat tcctcatgtc
     3781 acctgttacc cgtcacctgt aatcttataa aatcgagttt aaagccagaa ttcgcccgaa
     3841 gggcgttaag ttccattgca gttgattgca gctatggtgg tcatggggca tttagtgaga
     3901 ctttaaacct cttagcgtat atttacgcac gaaactgggc gatacccttg atactatttt
     3961 tcagttatct cattaaatcc cataatatca aataacctaa tctccttttt tgtagatttc
     4021 tcccgttatc atagataaag ctgttttggc agactccctg gcaccatatg atttatcatt
     4081 tagttgtttt cttagctctt ctattgcttc ctctgccttt aaagttccaa gagcaagagc
     4141 cgtagcttct ctaacatgcc attgtcggtg ttttagggat tttctgagtg cttcgatagc
     4201 ttttttatct cccagtttac ctacagcttc tgctgcctta cactgaatat cccaataatc
     4261 atttaaaaga agttcacata gaaaaggcac tttatcaata tatccactat taccaagttt
     4321 ctctatagct tccaatcttt cttttctatc tttaattcta tcagagcttt taagaaattc
     4381 gataagttta tctacttctt ctttaagcaa tttaacatat actgttactg aaaacagttt
     4441 tcctcctttt aagccaaata tccagagtct ttctaaatta taataatttt acaaagaatt
     4501 atttagttta tattctatgt ttttttataa aaatcctttt atagaatatt tttgcccatc
     4561 atcgtgaaag tacaatagcc cataattgaa taaattttat attcataaac ctattctacc
     4621 cctaaagctt tgaatgctgc atctgcaggg tcgttataat ggattaactg gagttttgtg
     4681 aagagctccg gcggaactgt gactagttcg accatgttac ttacagggat gaggactttt
     4741 tttgctccgc tatcaacggc gatctgcagg gcatctgcaa gggagtctgt ttttattatt
     4801 gttcctccta tgctcattga gcctatgact accatctggg gttgaagggg ttttcttgtt
     4861 atgccggaaa taagggagag aaaaactgcc agttctattt cttttgcttc tcctatgccg
     4921 ttcatatcta ctgctttcat atgaaaatct gtttctgtgt atttgctttc tcctgaaatt
     4981 ctggtcaggt ttgctttgaa gtaattttct ccctctttta attcatcacc ggttatcttt
     5041 tttgagccga aacctgtatg actgaatttt ccacttcctg acattgtttg aatttcgagt
     5101 ttgtatagtc ctttatggcc tgtttcaata cttttggcga tcacatagac tattccgggg
     5161 ttttgtcttc cttccgggat aagatttctt cctccgactt ccggtaaagt gataaatttt
     5221 tcttcttctt tttccgggtc aatgtaagaa aaatggacat cgtaaaattc cattcctccg
     5281 atttttttta gctgttcttt aattcttctt cttcctgtaa gggcgtattc caggcagggt
     5341 tttaattctt cttttgtaaa ttccccatga ggatatagta ttttcagaag gccgcttacg
     5401 gtctttttta ctgctattac atcacgctgg ttaagattgt ttccaagttt gtagtattta
     5461 tttatggcat cggcgaaatt cttttttctc atttcccgga accattctgc cagatagtct
     5521 gtaataaaac cgaattgatc tgtgaagaat tccggtctca tttttggaac ttcccatccg
     5581 ggaaggtaga tatggaatct gtcgaaaaag gctgtgtcga tcatttcgtc agggaatggt
     5641 gaaagaagat gagatgtttt caggagatgt tcgataggtt cgtttatatt acctatgaaa
     5701 accattgctg cattggcttc tactggatct tttcctcttg caaaagagcc ggaagccata
     5761 taatctttca ttatctggac tccgccttta tctttgaagt ttatgcctgc tacttcatca
     5821 aaggctacta tgtcccataa gcctacaagg cccactcttc tggttgccat attgtaaaac
     5881 agatttgcta ctgttgtctg cccgcctgag acaagtattg agttgggaga catttctttg
     5941 tagacgtagg atttcccggt tcctctgggg ccgagttcgc acatgttgta attattttct
     6001 acgaaaggta taagccttgc tattaaatgc catctgcttt tttcatccag attggcaggt
     6061 tccattccaa cacttcttaa tataatatca atccattctt tagtgttaaa ggactttctt
     6121 cctgtaaaga gttcattcat ttccatgttc ggcatctgta tgggtttaag ttctcttaat
     6181 acaaagggac tgtcttttgc cgattcatcg tagaagtatt caattgtaag gatacaccat
     6241 atgcctcctg caaggagttt ttcaaatttt ttgatataat caggagaggt aacaatttct
     6301 ttgagtccca gatttgttaa aactgcttca taccggtctt ttttttcatt caaatggaca
     6361 gttactttat cgataacttt ataacgtcct ttttctctta tatgagattt aatcttttca
     6421 gcttcatctg ttctgacata gttatcagag agaattccct ttactttttc aagcccccat
     6481 tttaccgttt cttccacatc ggaagaacag tacataccaa gcagatattc aaggacataa
     6541 gtaggaacgt tggctccttc ttttataagt ttggtaagat cttttcttac tactttaccg
     6601 gggaaaagag agagtaattt tttattaatg tcttccattt acatcaccat tcgtcaaatt
     6661 catttccaaa agctatatgc atatcataaa gtactttctt atattctgca tattcatttg
     6721 tgttttctat gagttctttt agttttaatt ctacaggctg gccattataa tctgatgagt
     6781 cacttttaaa tctgaagata catttttgtt ctctgtttct catatcatct tcttttgatt
     6841 cgaagaggag tgtgttttcg tcagatatta aaattccatc tttagtgaaa aatccgcatt
     6901 ttaattttct gggcagcacc ttgtctgata gaggttcttt ttgcacaaaa ttgaccggga
     6961 attgattact tgtaattgat gattgagtct ggataatatc gacttctact ttttttgtag
     7021 tgctttctct tttacatttt actttgatta caggaactac tatttcctgg aggcttgaac
     7081 cgccatggac aaaacgactt cctcctccct gggttttcag tctttttatg gattttgcta
     7141 ttagaaaatc ataatctcct gaaatattta attcagaaga ggtaaatttc tttgtagggc
     7201 ttatttctat gagatctttg cctattaaga aacggcggtt cttttttaag attgttccac
     7261 ctgtatctgt gattttcttg aagtctatgt cttccggttc tctgtgctgg tataaaaatc
     7321 cgtgatcact tgttataata atattggttc cgttaaaatt aactatatgt ttgatgattt
     7381 tttccattcg ttcaaaggtt tgctcaacgg catcgaatac atttaattct gttttgaaat
     7441 catcacctgt ggcatctatt gtgtcatcgt agatatagat aacattatat ttttttgcaa
     7501 attctcttcc tgtgtcacgg gtaaaagaga gaaaattatc agcttttatg gctgtgcagg
     7561 atggattgaa attttccagg actttctgtc tgtcatcaat ggactgaata gactggccat
     7621 caataaggaa tctgtctttt tccatatcac aggaaagctc tttatgtgga agaagagctg
     7681 ccatacccag agcagtatag cttggaagca tggataacat aaaggaaagg tctccttcaa
     7741 gtttatcttc ttttagaatt ctttcataaa gttctgctcc tgtttcatag cggaaagcat
     7801 cggaaataat aacaaaaatc ttctggtctt tttttaagta gggatttaca caggtgtcaa
     7861 aaaagctatc ctgtcccggt attaactttg atttccattc tttgcaggag tctacatatt
     7921 tctgccattt atcattcaga gaaggaagaa atacacagga atatatattt tctatagtgc
     7981 tgtccagttc ttttaaaagg gtagtttctc cctgattttt ggaatagtag atatattttc
     8041 tgtagagagt atcgaattta taaagagatt tactgtagac agagagtcct tcgtcaaagg
     8101 aattaaagtc aactgccata tttttaattt tataaatttc cagagccata ttcagtgctt
     8161 cgtatatctt ttcatattct ttataccaga gggatagttt tcttttctgg attacggaga
     8221 tagcttcgtc tatatttata atttctttta ataatttatt ttttatatcc tgtatgattt
     8281 tttgttctac agattcaaga atatcacagt ctttcaggtc tctgtagtca tatgtaagga
     8341 atttattttt cagtccgctt tctttttcta ttttaactac cagagagata agactttctt
     8401 tattttttga actgtccatc catctgttaa ggaatataat actgtcctgt gacagttttg
     8461 tataactttc ttttacctct gcctttaaag aggagtaaaa atggaagagg ataattttta
     8521 tgagaagatc ttttattgtg gggttatcag ttttataatg aaatgtctcg tctatcattt
     8581 cccagaaata ttcatttaat ccgtattttt ctatgttttt ccatgagtta tcactgtctt
     8641 tatccatttc tgtaaagaga ctgtaaagta tctcttctat ctgaggaata gtgcctgtaa
     8701 tgacagatat catctttaaa gcaagtcgtt tctgttcatc attttccgag atcagacttt
     8761 tgagtttttc tattctcttc tgacttttaa agaaagaaat atgttttttt ataaaactct
     8821 tcatttcaag gccgagtttc agatcctgta ttatcattga agggtgatct gcagaaaaag
     8881 agatatttgc tatgagaaga tctataagcc agtttgtttc tgccggaggt tcgctgtaag
     8941 gggcatagat gaggaatttt ttatctggct cctggcagat aactttatat tttatataga
     9001 attcattgtt ttttatttcg attttttcta tatcagacag gtcaatgtcc tgataaatct
     9061 gtctcatatc tccttcttca tcgtaccaga agataagacg atgggtttca aataactggt
     9121 taagtctttc ttttattttg tccatatttc atctcttcag gagtggattt cactcttcag
     9181 ttttattatt tcctcctctg tcaatcctgt tacatctcta attatttctt cagaaagtcc
     9241 catttcaagg ctttttctcg ctatctttac ttctgctttt tttctgcctt ccataattcc
     9301 tttctccata gctttcattc gtgcagtctc aagaatactt tcctcatcac ggagtatttt
     9361 taatttttcc tcataaacat ctctctgttc tatagttaaa ggcagatgtt ttaacacatc
     9421 gatgattttt tctattgtat tcagttcatg caaaggaatg ggaagaactt tgtctttgtt
     9481 tctgtgttct ctcataactt tttcataaat actttctttt tcacagctat ccattttcag
     9541 gctccttatc ttttctaatg agacagcatt ttccaaataa ttgacaggat tttttttctt
     9601 atttccattt atcaatcaac atgtttatct cttcagaagg taatcctaca tatttacaga
     9661 ttagttctac tgataatcct tccctgagca tattttttgc aacttcaatt ttgccttgta
     9721 tccttgcagt ctctaaagcc ccatcctcat cacgaagtgc ttttaatctt gattcataga
     9781 tttctctttc ttcatctgtt aaggatattt tatccagtat tctggttgct ttttctattg
     9841 tatctatttc ttttaatgcc acaggaagat taagttcagt gaacttttct gcatatttca
     9901 aaaagtatat ccatctatca agagtctttt ttaaatccgg cagttctttt gtgaattttt
     9961 caagttctat gaaatgaatt tcaagctgat catgaaggtc tttaccggtt tttaagtttt
    10021 ttatgcgata aatactgtgg taatcttttt cttccagtct gttgaaattc aagatattta
    10081 tacttacagt tctctttaaa ttattatacg ctctgccttc tcccagttgt gatgaatata
    10141 gcctggccca gtaataggtt gctctcttat tgtaataata ttgatctgct atctgcattt
    10201 caatattgta aaattcccct ttatcatctt ttgctcttat atccagtatt gtcagtttgt
    10261 ctctttcatg ctctttactt ccaaagggat taagtatttc tattgatgct attggtcttg
    10321 tctgatagtc ttcaagtatg gaatttaaaa gagatatgag tatatcttta ttctgttcgt
    10381 ttccgaatat cagtttgaaa gcaaaatcta cacggggatt tattttacac ataaaattca
    10441 ctccttttcc agtcctgcta tggtagcaag gacatctttg aacttacaat agttaactct
    10501 tactccatcg tcaaggtcaa tgggaatata ttgttttgcc atatggtcca gctttttatc
    10561 atattctgcc aggtctttaa ggattttatt gatttccttg atttccttat cttttttagt
    10621 cttttctttt cctgaaagag tatccgaatc tatatccttt ttaatctgaa cctttctgta
    10681 ttcctgtttt gaagcgtatt ctctaagata ctcatttaac actttgctac agagatcagg
    10741 tgtgtagcgg tgcatataga ttaaagcctg gaaatacttt ttcggagact ggaacatcca
    10801 gtatatagga cgttttctat atctttttac atggtcgctg taaaaatctt tttcaaaata
    10861 ggctctgatg gattttccga gtttttcttc aataaaagca aggttttcgt ggaaagtacc
    10921 ctctccgaag gttattttca ggaatttatt gaagagtgac actatgtcat tttcaaaata
    10981 ttcgtctgaa aggattggga ggatattatc ttcatcaggg ttaaatgaag gagcttgatt
    11041 aataattttt atataatttt caactgtttc tccctgattt gcaagtataa gcccttcttt
    11101 atccggggaa tatcttccga agatacatcc cacactgtag gagatgaact gtttcatcac
    11161 ttcatccggt ttgaattcaa gttctcctct gtcgttaatg aaggtttcat ctctgagtat
    11221 agtaatgtct tttaattcta cttcgggagt aagttcatcc tgaaggccgt atatttctat
    11281 gaatatctta tttaattctt cttcatttct gtgaagggtg cagaattggg attcagcaga
    11341 ggatttccag attttgtagg cttcttctat tttttcattt ttacagagaa taagggaatt
    11401 acttttaaag tcccagcttg tttcacgtga gttccagtca gttcgagaga tggatatgtt
    11461 ttgttctgaa agagtgtcta tgatgatttt aaggggttta ttttctgtaa agattattgg
    11521 tagagatgat atatttccaa cctgataatt taacgttgga tttaatattt taagaaaatc
    11581 acaagttaat tttgaacata aaaaagctgt agtatatttt attttatctt cttttggaaa
    11641 caaggatgag cctgccacgt cgaaaagaaa cccttcaggt gagaatctta ccccgaagtt
    11701 tgaggaacta atatcagtcc aagtaatagt ttctttaaaa taaaaatttg gatctctcaa
    11761 tctgtaattt gtttctttct tgatttcttc accattattc tccatattta ttatatgaaa
    11821 attatttcca taccattttc taaattctcc tcccttgtta taaggatacc attttttctt
    11881 ttctattagt atatctgaat aatgactaaa accaatacct attttattaa aaaatacttc
    11941 gtaccaaaat ctcacaaatt tatcattatt acctgtttga attcctgttt ttacatcaaa
    12001 caattccctt attttattag ctttttcaaa aatttccttc attttatcac ttgcccagta
    12061 tgcaattgga tttccaggaa tttttttaaa atctttctga ttagctttaa atctataata
    12121 aatatcagat tttttaatcg cattcaatgt tttaataggc tgattttctg ctccagtaaa
    12181 ctcagataat ttaatataat caccttttat atcaaggttg ttattcctca aagtaaatgt
    12241 acatatagga acagtagcct cagcaaagcc agaatattca agttgaatta aattagaaat
    12301 cactttgttt tcaattaaaa atactcttaa ccattcatag aagtaaataa acatccaaac
    12361 aaatggggtc ataaagccta atatactatc attttttccc atactacaac atttcaggat
    12421 aaaacatgca aaaatatcaa attttgtttc tggataatgt ttttttacaa attcattaag
    12481 acttctttct atatatttca aattcatata aggcggatta gtcaccacac aatcatactt
    12541 ctgccataaa aacctagcct gcttccttaa attctcctgt acaacctcaa aaagactccc
    12601 tctatcttcc tcaagtctct ttatatcttc ctcacttacc ctgataagag aacccaaaac
    12661 cttcgcattc ttaaatatct catactcatc agaatccata aaagccataa cattcggtac
    12721 aatcccttta tcaaaaaacc ttcgataata acctcttgcc ttcatcataa gagcaaaaga
    12781 agcaaggctt gccgctctgt cgtctatatc aattccatat aaattctttt ccagaattaa
    12841 agaaggtatt tcactcttac tgtatccctc ttcttcatat attttaatta aaagatcaaa
    12901 ggcatagaca agaatatgac ctgaaccgca ggcagggtca aaaatcgtta tttcttcagg
    12961 cgagtttatc tttcttggag gtattttatc tttatcagca ggttctatat aaaattccat
    13021 aaactcttta agacaggaat ccggccttgc ttcaagccat aactgcccta aagtattatc
    13081 aatcatatat tttacaatcc atttaggagt aaataactgt gtggctgcag gaatttcatc
    13141 aggtttatat ctcttctttg catttattac ttcatccttt ttctcagaaa tataatactg
    13201 ataaagccac cctattatct ccacatcttc acaatcatcc tcagaaatat tctttacaat
    13261 ctcatctctc acagaaccgg ccttcagtag attctcagga ataaggagtt ccgtataatc
    13321 atcaatccat tcaaacataa aaggcataat tttatgataa taattgcaca cccctgtaat
    13381 aagcatcctg tagacttctt cttcaggatc tctactcctt atcttcccgt ccagaagatc
    13441 atttatctta ttcctgtcca ggcggagttc ttcatcaata agtcctccct ttgcctgttc
    13501 aagtatttca gggactgtcc ttcccggcag aggagacaat attcttgatt tcgtataacc
    13561 cttcagttcc ataaatctga gtgccacaaa acggttaaac catatgtaag agaccttttc
    13621 tattactcca tcaagaccaa tagaatcaat ttctttttta agatcgccta tctgtttttc
    13681 tctgtatttt atttctatat cttcacctgt gaggtaatag tttattttca cctctgtcat
    13741 ttctctgagg cggtttctga gctgtcttgc aaaaattttc aggttattct tatccatttt
    13801 gtatcctttc aatcttcaga tttttagcta aagactatag agatattatc tgcttctata
    13861 aggaatcaga gataaatttt ccttttattg ttcacttcat caaggagctt ttctcttaaa
    13921 tcctttacat aattttctac atcatctgct gtttcaagag tggttttttt ataagagagc
    13981 ttaatttttt taatatttac tatctcatca gggattacgt tactcttttt agcaaattca
    14041 gcggctattt tctttaactg cttttcataa agctcatcaa gtttccctga cttatcattg
    14101 attacaggaa taagttcttc attaatgcct tttttaataa aatcttcgaa aggtttcaat
    14161 atctctttct gcatatcctg tgatatttgt ttgtaatctt cataatcttc cagtttaagg
    14221 atataactcc gtgctttttc tataattctc ttttttgctt catcaattaa tggagttaga
    14281 gaagatttca aatcttccag gagatttttt gatttctgca tatagccagt tctgtaaggt
    14341 ttcctgtctt ccattaccat cttaagagat cttatttttt gttcatcaga aactttttcc
    14401 aggttaacct gttgagatga gataaacttt tttatgtcat catagatctg tttctgagga
    14461 ctgttcataa agccagtaac aggctctata acatcttcat taatgtcgag tatatgcccc
    14521 attttatctt cgattttatc gtaaaaatca ttcatacctg tacgggcgag ttcttttaat
    14581 tcatctacta gctctattaa ttctgttaaa aaatgatatt ctgaggactt accgataagg
    14641 atctccagtt tacttatttc ttccttcagt ttatttctga attcttcata aacatctttc
    14701 tggtcattta cagtgagtga tatattgaag agttcctgat aaaattcttt tgctttagca
    14761 atctttccct catcgacttt actctgaatt tctactgtaa tactgtcaaa ttcttttgag
    14821 tttaaaagct ttttatagat ttctttataa tcctgtagct ctaccgagtt gtaatttatc
    14881 ataagtttct ttttagaata taaccctgca atttgagaca gaattgcagg ctgataccag
    14941 ccgtagccgt ttcctgtaaa attttctata atatgtttta ctgtaaccct tccgtaaggt
    15001 ctttttcttt caatgaaaac cttcacttcg tcttctcctt cagtgagact tccgaatagg
    15061 tcactcccac tgaagagtat ttccttaata tcatcttctt tgtaaacttt agtaagcatt
    15121 ttaagtttcg ggaaggttac ttccacagct ttttgaaaag cttcattaag acggggcttt
    15181 acacctgtca tattgctgat ttttatttct tctccattca gatagaatct ggcatcattt
    15241 ataagatcct ttatctgttc gattagcttt gatcttcttt cactattctg tgaagctgtc
    15301 tgctcaaata aaagcctctt ccgatcactc atagaggtag agcttttttt aatatatttt
    15361 ttagtttgaa taataagtat caactgattt ctcaattgat ttctcgtatt ttccagttca
    15421 gggaggctga caattatgtc gttacatccc atacttttgg ttttcagttc catatccgtg
    15481 agattttttt cttcaatata aagaggtgtt ataaagtgaa taccgagaaa atgctctttg
    15541 ctgtagtgcc gatcatccat tttttttgta aagctgtagc tctgttttat tggcttataa
    15601 tcaaatttag tatctccaat aatttcatca aagactattt ctgcaagaga tgacgatatt
    15661 tcagtatctt cagtatccat ttctttaatt tcttcctgta tatccttctc ttcatctgtg
    15721 aggtaatgat aaatgcctcc tatattctgt atataaattt ccctttcaag tttttcaagt
    15781 gaatttttta tgtttttttc cagagctatt tcatctgtat caacactgtc tataagtaaa
    15841 gtagtaatat gctggacatt tgttttaaag tgatcaatat acctgaccag aaagagggat
    15901 ttcaatactc gaaggtcaaa aggcttcagt aagtcgttct tctccgcatt atttaaaccc
    15961 agaacagcct ctgttttgag cactcctttt atagatgaga aaaaaaggtc aaatggcgca
    16021 agtgatgaaa tttctttact gcttaattcc attgcagaaa gttgatagac ttcaagcatt
    16081 gaccgttctc caaaactctg gtgttttccc agcagggcat tatgcctgcc aagctgtatt
    16141 attgcgtcct gcatcagttc atactgatat ggcagaaaag gataatctgc tataaaactt
    16201 tctttatctt ttacttttgg aaggttctta cctgcatctt taaagtggag tgatttaagc
    16261 ctggtttgat tatcgtgata gaacttatca agtttctcag ttgattcagg agttttggca
    16321 aggagtcttt tctgaataac ttcatctaca tttttcgagg tgaggtttaa ttttgtacga
    16381 aaacgcccca tgatctttga gaaatcctgg ctttgacttt tagttatgtc tcctactatt
    16441 gcttcaaggt tatgctgtga tgatactata agccatgcct ttccttcaca gattgtgccg
    16501 aaggtttcgg caatagtctg gaggttaagc attaaatgac tgtcattgga tatatactgg
    16561 cctacttcat ctataaggaa aataagcctg aatttatccg gctgtttatc tatatagtct
    16621 tttattatat atgcgaaatc ctcaatggac aggtttttct gattttctgt tactgttctg
    16681 aatacttctc ttccgtcttc ctgggagata ttttttgtca tagaaagggc ctgtataaat
    16741 tcagtgattt ttagatcaat tacttctcta tcttcttccc atgtttctcc ttcagatact
    16801 ttcctgtaat tctctttaaa tttttcgtat aaattcaatc tgtcaagatt tctttcaaat
    16861 tctgcaatat tgaggcttct gtgataataa ccggaaaatt cgttaaagac tttcattatt
    16921 acagaaagaa caggctcttt ctggttattt atctggtctg ctttttgatc tatattgaaa
    16981 agaatagttt ttacaggtat ggaagaagca gattttatat ttgcagcaag ctcaaagtct
    17041 ccgtttttaa ttttattcag gaagatatca actgtttttt taccatctat ttctctattt
    17101 tcaagaagat atgatagaat tttgagaaga tgtgatttac cacttccgaa gaatccgctt
    17161 acccagacac ctgtatcacg gctggtcttt gatgatatgt cttttgtata aaccctgaaa
    17221 aaatcgtcta atttttcttc tacttcattg gtgataacat attccttaac ttcctgggtt
    17281 atatggctgt aatcatcggc tttgattact ccttcgattt ctcttgatat atcctgttca
    17341 aatatgtttc ttattaacat cttctcccca ccttcttatt ttatttgtta cgctaaatta
    17401 aatgctctgt agtagttttc agcttttatt tttccgaaga gacttaagga ttggcctgta
    17461 taaataccgg gataaaaaat tactacaggt atatctttaa ccctggattg taaattgttt
    17521 aaaaggttgt gtactctgat atatggatat accagtccag tgcctgttat aaaaagaaga
    17581 tttttactgc tgtcctttac ttttttttca atggcaggtc ctattttagt ttcggcgtca
    17641 agagcacctt taaggttttc catcaatttg tcttttgtaa attccggctc tttttttatg
    17701 aataaatcca gcagttttct ttctttgagt atttcgatac agagggaata taaatttatg
    17761 tttaaaatgc ttataccgtc tttcaggagt cttttttcta acattttaat ttcatctgat
    17821 acgaaatatt caagctgtgc ttcataggca aaaatataga agggaatttc attgccggta
    17881 cacctgctgg taaggaagtc ctgagtttta aatttttcat aaagatcttt aaatctcgct
    17941 acagtaattt ttttattcat tggaaaaacg ctccgcatag tttgtaatat cagtgtctga
    18001 tagaaggaat attttaaaat atttatcggc gtgtttgtca tgcctgtaga ttaatttcag
    18061 tagttgttca tctatatagg gtctctgtat ttgtctttct tttacactat ttaaaagact
    18121 tccctgttca aggattttta agatcatatt ttttgttttt tttgctgtct tatctgttat
    18181 tggagagagt aattctattt cttcttttgt tcttataaat ttttcatagt ccatatccag
    18241 aagagtgtag tcaaagagta agtatttttc cctgattact tcaacgataa agtcgtaaat
    18301 tatgtgactt gctttgcaga ttgcatagat aagagtaagc tttgccatat cttgagttgc
    18361 atttaccata tattcaagta aaggaaagga taagttttcc agtctttttt tcagttccct
    18421 gtattctctt tttgtagtgg aaagagtatt tttttgaagt atattttctt caatgactgt
    18481 tttctggact tcttcccagt ttttaatctc aagataaagt tttgatattt taattgattc
    18541 gtttggaaat aatgaggcgc cgataaagga tagattgtat ttcataatga taaaatttaa
    18601 attccatatc ttttgtcaaa ttaaaatttt cacctgatgg tgaggtttta tctcagattg
    18661 agatagtaaa gttgtgtatt ttaattcaac aaattttttt ttaaaccttc aagtatagta
    18721 attgatacca taaactctta ccttaaagcc ggactaaatc tttataaatt agctaggaaa
    18781 ttcagtttat cttattgatt ttactaggtt tttttatagt ttatgtaaga taaaatttta
    18841 agtatttctt atgctcttca aaaccattgt aaacactgga tttgcaattt taacaaagtc
    18901 attaaatagg aatttaacga cattatatca aagaatcgtt tataccaagt aaactctttg
    18961 taaaaatgga catgcaattt ttaataaaac ccttaaaaac actcaattat tataactttt
    19021 tcatggaaga tttaccctgt ttgtttaatt atccttattt ttaggtttat ttaaagcttt
    19081 gttccttgct tcaatctcat caagtaacgt ctttatcctg ttattatatt cttccc
//
LOCUS       DAOQWT010000002        12772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_5401_length_12772_cov_6.200834, whole genome shotgun sequence.
ACCESSION   DAOQWT010000002 DAOQWT010000000
VERSION     DAOQWT010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 12772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12772
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5401_length_12772_cov_6.200834"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PKJ95_00070"
     CDS             <1..523
                     /locus_tag="PKJ95_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64669.1"
                     /translation="PAGNQAVETLLDNPAPWMRRQAVVYLYRYQSEQSEALLLDALRD
                     GEHMVWTMAEGALYGLWAKSDNEQADRNYRFGIHLQMAGQWNQAIPWFRQAFQEDPSF
                     IECIHQIGHSYEQMGQYREAIGAYLETIELKPSHFSALARLHALYEHLGNRELATHFE
                     ARTEAIFPHWEKI"
     gene            651..738
                     /locus_tag="PKJ95_00075"
     tRNA            651..738
                     /locus_tag="PKJ95_00075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:685..687,aa:Ser,seq:gga)
     gene            838..1071
                     /locus_tag="PKJ95_00080"
     CDS             838..1071
                     /locus_tag="PKJ95_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HNR64670.1"
                     /translation="MNPAVKKLTKEILSLPLDDQVIIAQTVWDRVEHFSDPEIEQAWM
                     EESERRWKEIEQGQVQCLSAEEAMNRARANLAR"
     gene            1068..1367
                     /locus_tag="PKJ95_00085"
     CDS             1068..1367
                     /locus_tag="PKJ95_00085"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNR64671.1"
                     /translation="MKIEFHPDAADELIAAAQYYEDQQDRLGQRFLDSIDEALSFLKK
                     NPEVWHSDERGRRKQRVKRFPYLIVYKYEQGRVLVLAIAHAKRKPGYWKERDGVR"
     gene            complement(1426..1932)
                     /locus_tag="PKJ95_00090"
     CDS             complement(1426..1932)
                     /locus_tag="PKJ95_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64672.1"
                     /translation="MQLTQEFMPEFSIAMAVIRHTTAAEEAVSRKKQRDEQLKATAGV
                     IQGIITTHTDKIRKVIDNICKKKRILFTFETADSQISEKIWERKIGINTFDQKLCKPI
                     ARITVDCTTPRINIKVEKDAECPKIFTTRINAAIRNASCRNQEPSPIRKGPDFRNHDI
                     LREVSQQW"
     gene            complement(1955..2482)
                     /locus_tag="PKJ95_00095"
     CDS             complement(1955..2482)
                     /locus_tag="PKJ95_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64673.1"
                     /translation="MEFRPFCLDTVTDSQDLRIKEKKREESGMYIEDNGKMHPVFKKN
                     SGFICPEISNGDYYDDIPSLIAFCKGPVNLTLHFKDNKEKLIDRLKNEIDVYQWMIDE
                     AKFFLKEAEQEGVIGISIKGDITGKVEYSINPEIDPHALMALKYIAKHTKDEERKTKL
                     KAWIKEIEYIFYSGK"
     gene            complement(2522..2947)
                     /locus_tag="PKJ95_00100"
     CDS             complement(2522..2947)
                     /locus_tag="PKJ95_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64674.1"
                     /translation="MNERIKNLLLSIRKWIINSKIKTGDIYLDYEKIEIKDHWSLNGK
                     FEPITEEEFREVYELCAIKEKSEYEEYEKRRFLGEQAKKDFYDEPERGDLLMDSEIDR
                     LKEEIEYRNKKIRELQDRVRELEEENEALNGTLEAWRDQ"
     gene            complement(3036..3374)
                     /locus_tag="PKJ95_00105"
     CDS             complement(3036..3374)
                     /locus_tag="PKJ95_00105"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNR64675.1"
                     /translation="MFKRLLLISLLLILLTYNAEPKSRYWEATFEITAYTHTGNTCAT
                     GIWPKEGIVAVDPAIVPFWTKLHIPGYGHAIAADTGRDIKGYRLDVFFDTYEEAVNYG
                     VKTKKVRVYL"
     gene            complement(3378..3752)
                     /locus_tag="PKJ95_00110"
     CDS             complement(3378..3752)
                     /locus_tag="PKJ95_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64676.1"
                     /translation="MFYMALDEIEQKRLFEEQEKEKAEDKKRKQKERLVHKIPGKKNP
                     SISELREYAYDRFKTRDNKFSDFPVGTAVQVIVPCADFRFFDFQTGVVIENSGKYLGI
                     RVKFDDKDEDAWGFDPKQLRRI"
     gene            complement(3754..4209)
                     /locus_tag="PKJ95_00115"
     CDS             complement(3754..4209)
                     /locus_tag="PKJ95_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64677.1"
                     /translation="MKTREHLKEALIKEIKKLNKKITLDRKIRTLKQTAFLYAILVCM
                     EYYMEKPGFNGMWEIRNKEGEVSCFSFLDAARLLEKSSNMQEFLDNLKMSIPTHETRT
                     RDYIIEKVLDTIRPYYSLISTGNELLRDLHLTIEQATIPKIDELTKIGE"
     gene            complement(4213..4779)
                     /locus_tag="PKJ95_00120"
     CDS             complement(4213..4779)
                     /locus_tag="PKJ95_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64678.1"
                     /translation="MVDNKVTICRVYDHNEERETETEEIGILVLTNGTITYIPENKTW
                     NAKRDTLMQYEDIIHCIIDFNGKSFYLNEADYPVIEKLVKENIDYCLKNLHIPSKQGI
                     TCSTYGEDPEVGFFSTSEGDYCTDCYRKKFSTIKETATPKRELAGVPEDKKVTCVKCG
                     EEISERKVFTVDGTYICYHCYYKAKEEE"
     gene            complement(4773..5057)
                     /locus_tag="PKJ95_00125"
     CDS             complement(4773..5057)
                     /locus_tag="PKJ95_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64679.1"
                     /translation="MPTFKEQQEEIIKLKKEIEEKIKTLNEKYREITENLKLGMAYSD
                     LTAEKICVVESIKTKDAQFPYRQLYVSDINGVGLTPIERVYLKEGGKRTW"
     gene            complement(5044..5277)
                     /locus_tag="PKJ95_00130"
     CDS             complement(5044..5277)
                     /locus_tag="PKJ95_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64680.1"
                     /translation="MKISAETVDGKIKLLFFVDLTCEHCNEPLTEEDIKEERELSKGV
                     TNTCSICGAKIKTVVTMFEGPEPKKEGETDANI"
     gene            complement(5459..>5970)
                     /locus_tag="PKJ95_00135"
     CDS             complement(5459..>5970)
                     /locus_tag="PKJ95_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNR64681.1"
                     /translation="GADLRGADLCEADLRGADLRGANLYEANLRGANLCGANLCGANL
                     RGADLCEAKNHLPQLYCLKQMPPKTKLTLYKYVTKDFKSPTSDNKITYQVEKYYIVEE
                     YETDERIECGAGLSVATLQWCLINKDEEDIILECEFLSKDIVAIPFATDGKIRVKKIK
                     VKKIWKKEE"
     assembly_gap    5971..6070
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6242..6460)
                     /locus_tag="PKJ95_00140"
     CDS             complement(6242..6460)
                     /locus_tag="PKJ95_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64682.1"
                     /translation="MVSFIEDIANVLERHGKCKNMPAKKVAKFIESSLRALDKVTGQE
                     EEKSTPKKTMSDFKDSPVSMSATTAVKE"
     gene            complement(6499..6801)
                     /locus_tag="PKJ95_00145"
     CDS             complement(6499..6801)
                     /locus_tag="PKJ95_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64683.1"
                     /translation="MPTWTITSEQVEKLTDEGLKQYLDENKKGGNTLIIKNCIVEKFT
                     PKGPNKDDECITTITIKAVNIPNSQIAELADILGAEVDIEIKSSIEDRNGQYKMNI"
     gene            complement(6805..7617)
                     /locus_tag="PKJ95_00150"
     CDS             complement(6805..7617)
                     /locus_tag="PKJ95_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64684.1"
                     /translation="MKEYERIYTTHFGDSYSNGKIPVQDIRINGKSLGRKGTRVILNE
                     EMEPCEREGISRLKRSIDIIIEKPKEDKKKKEEELKNWKNTMKKELLFTIGKLFKSIA
                     LSVNELEIQGFIKHISVLFPMETEKTYGITVDKEKKQALMINSLFEYFEEIDKLKGKD
                     IPRVQKMIKEIQENARAFKAEVKAPPIMGEKVQACTKVKEDTKTIADLLQEDLEKRCK
                     DRFKHEEKSRNSGKTQEMKDYVFFGIDFAKGKGKTQAFNVGFKNGKIVIQEI"
     gene            complement(7633..9063)
                     /locus_tag="PKJ95_00155"
     CDS             complement(7633..9063)
                     /locus_tag="PKJ95_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64685.1"
                     /translation="MAYNYSEEGSAYALVPLDKAATLAVRNIEPAMPVEMIDQMFHMQ
                     INPKTGKFKLVIDKAGLNWKMDQLYRGKYDLKTDVLMGEEYDFIKTNCLGFKKEDRVI
                     ICKSELRIEVANGKEKVFTAYGTATPDNVKKGQKHPLELAETRAEKRVLQKATGCGFS
                     AADCDEFLPTTPEIDTKELIEAQRKRMWVLLKDAGYKEEDRSGRISFVSNCLGRKIES
                     TKGMTYADYLKVNNTLETIKKGEIPPPPECKGDIIDGDYTITEEPPLPPPSPGITPDQ
                     LNQINNLYIEAKWDTEKQEKYFAYMREKNPDRFTTLKTAADFSQEDAEKFISLLKKKV
                     IEADRKAMEIPPPTPAPEEAPIIAPQERHAHVPASYSQIAEADELFKQLEYDWQKKGR
                     FFEKYRYETGETYVKSVKDMDFIKAGLLIDHLKKELAEKHKMENGQFPGGVTEEENEA
                     EKQDIRDNTIPTSEKMQDMFDGDVPF"
     gene            complement(9069..9215)
                     /locus_tag="PKJ95_00160"
     CDS             complement(9069..9215)
                     /locus_tag="PKJ95_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64686.1"
                     /translation="MTVKKKNKKTSSVTIRLSEVERDALIKEAKEKGQKLSAYLREKF
                     EKKE"
     gene            complement(9285..10688)
                     /locus_tag="PKJ95_00165"
     CDS             complement(9285..10688)
                     /locus_tag="PKJ95_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64687.1"
                     /translation="MQKTYFREKHSRHLWSLECGEKGEWKVSYVQIGACLETKEALDL
                     MFSFIAYNAETDKINIPPPEFYKTKSHIGYSGCRDFYAQTGGHIRYDIIPGTDIIQEY
                     KTPVKRIPLVYSSMNDKVNSGLTITWDTFWSVLQVYLEYFEKSVSEKDEGLITEQVER
                     WWGHYRECYETRDFEEVQEKGIELPVDTSYVKIKVAPDNSFFILSNNNSEYLKINTNI
                     PHEWNHCSLLRDDFFYNQSDIINALKERDIISYIIEDGEREKPHRWIFLNMLNNDWFF
                     IDKEGRVCERHNNPDVPCFFIPNKFEKPEHFQVYFCTKDNVICLENQGFWTRFDRYFF
                     NGFGEEELEKMIATLNEKIMCEVIHLYKTEHGAMPLVDIADNYIKICIRDKKLPEYKE
                     IQIGCQTVLYYPEIKRLHWQDYNIEIDGDPVDFEPGDFNLIMDHKKKVTEALKKYDLK
                     WMEIDGEIKFESIEIPF"
     gene            complement(10851..>11615)
                     /locus_tag="PKJ95_00170"
     CDS             complement(10851..>11615)
                     /locus_tag="PKJ95_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HNR64688.1"
                     /translation="CLNCYAEKMHKRLQAMGLEKYSEPFSVVRFCIHHLNIPSKTKKP
                     TVFFVNSMSDLFHEDLKKQYLFDIFREMVDNPQHIFLILTKRPENIPIWKNEKPYQWE
                     YYKRESYWTDNIWLGVTIESSKYLHRAETLQKIPAKHKFISFEPLIGSPLIPGVSVLN
                     SNYYKVDWIIVGGETGINARVLQPAYVSVLRDFTEYENIPFFFKQWGGKEKNAFLGGK
                     EYKQFPAEIAKLMEGKNSRVNSSRKMQNLPGNLHQD"
     assembly_gap    11616..11715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11716..12065)
                     /locus_tag="PKJ95_00175"
     CDS             complement(<11716..12065)
                     /locus_tag="PKJ95_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64689.1"
                     /translation="MPMEKKYGISRSILMTTEDDQILRKEAQKAGLSINQYLRAVIRK
                     DTHLRITEKDIENIKKYLMRQNCLYCREYGLDTLEESSFEKEVVQCQTCRRKMLYLPR
                     NGYYDLVPYDFFSQ"
     gene            12185..12457
                     /locus_tag="PKJ95_00180"
     CDS             12185..12457
                     /locus_tag="PKJ95_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64690.1"
                     /translation="MNCDFIPYKYDSDPDEECYLWSKYVFAVKYGYVCPLPEEQRFLL
                     DHIDIINRNLQKYKLYLHPSPDGINGGVYTSYSFVLRRIENVSTAN"
     gene            12438..>12772
                     /locus_tag="PKJ95_00185"
     CDS             12438..>12772
                     /locus_tag="PKJ95_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64691.1"
                     /translation="MFQLPINEFTTGDAIDGVKHIPEGEIDLIFCDIPYNISNETKIF
                     RDYRQANGKKKSANISFNFDEQEEGEEEDKWDHDFDPIPFLDASIPCLHSTGSWIIWC
                     AEQQHGHIR"
BASE COUNT         3102 a         2637 c         2191 g         4642 t
ORIGIN      
        1 ccccgcaggc aatcaggcgg tagaaaccct gctggacaac cccgctccct ggatgcgccg
       61 ccaggcggtg gtctatctct accgctacca gtccgagcaa tcggaagcct tgctgctgga
      121 cgcccttcgc gatggggaac atatggtttg gaccatggcg gaaggggccc tttacggtct
      181 ctgggcaaaa agtgacaatg aacaggcgga tcgcaattac cgattcggaa ttcaccttca
      241 gatggccggg cagtggaacc aggcgattcc ctggtttcgg caggcgttcc aggaagatcc
      301 ttcttttatc gagtgcatcc atcagattgg gcacagttac gaacagatgg ggcagtaccg
      361 tgaagcgatc ggggcctacc tggagaccat cgagctcaaa cccagccatt tcagcgcact
      421 ggctcgtttg cacgccctct atgagcactt aggcaaccga gagctggcga cccacttcga
      481 agcccgaacc gaggcgattt tcccccactg ggagaagatt taggcggact tcaaccttac
      541 ggtcccagcc gcctgatttt ccttgacaaa cgcctcggcg gtccgtagat ttcccccctg
      601 tccgttctat cgaggggtcc gcccggattc ccgcatagaa tggccaaata ggagaggtgg
      661 ccgagtggtt taaggcgcac gcctggagag cgtgtggggg cctaaagccc ctcgtgggtt
      721 cgaatcccac cctctccgat tttctccccc cgcatataaa gaattccacc cgcttgacgc
      781 atcatcggtc caagggtatt gttcgagttg tatttttggc tcttgaggag tgtctctatg
      841 aatccggctg tcaagaaact cacgaaggag attctgtcct tgcccttgga tgaccaagtc
      901 atcatcgcgc agacagtatg ggatcgggta gagcacttca gcgatcccga aattgagcag
      961 gcttggatgg aagagtcgga gcgacgctgg aaggagatcg agcaaggcca ggtgcaatgc
     1021 ctttcggctg aggaagcgat gaatcgggcg cgagccaatc tggcgcgatg aaaatcgaat
     1081 ttcatcctga cgcggccgat gagttgatcg ctgcggccca atactacgaa gatcagcagg
     1141 atcggttggg ccagcgtttt ctcgattcga tcgatgaagc actatcattc ctgaagaaga
     1201 atccggaagt ctggcattca gacgaacggg gacgaaggaa gcaacgggtc aaacggttcc
     1261 cctacctgat cgtctacaaa tacgaacaag gccgggtcct tgtcctcgcc attgctcacg
     1321 ccaagaggaa gccggggtat tggaaggaga gggatggagt caggtgagcg gcaagaaagg
     1381 cagcgagagc cgaacccata ggattggtcc ggcacacatt taatattacc attgttgcga
     1441 tacctctctt aggatatcat gatttcgaaa atcaggaccc ttcctgatcg gggatggttc
     1501 ttgattccgg caggaagcat ttctgatggc tgcatttatc cttgtagtga atatctttgg
     1561 acattctgca tctttctcca ctttgatatt aattcgtgga gtagtgcagt caacggttat
     1621 tcttgcaatg ggtttacaga gtttctggtc aaaagtattg atgcctattt ttcgttccca
     1681 gattttctct gaaatctggc tgtctgctgt ttcaaaggta aagagtattc ttttcttttt
     1741 gcaaatatta tctattacct ttctgatttt gtccgtatgg gttgtgataa taccctgtat
     1801 tactcctgca gttgccttta actgttcgtc tctttgtttc tttctgctca ccgcttcttc
     1861 tgctgcagta gtatgtctga taactgccat agctatactg aactctggca tgaattcctg
     1921 tgttaattgc atggttttat ctctcctttt ttatttattt cccagaataa aatatatatt
     1981 caatttcttt tatccatgct tttaattttg tctttctttc ttcgtctttc gtgtgtttgg
     2041 caatatattt tagtgccata agagcatgtg gatctatctc cgggttaatg gaatattcta
     2101 ctttaccggt aatatctccc tttatactta ttcctataac tccttcctgc tctgcttctt
     2161 tcaagaaaaa cttcgcttca tctatcatcc attgatacac atcaatttca ttttttaatc
     2221 tatctataag tttttcttta ttatccttga agtgcaacgt aaggtttact ggccctttgc
     2281 aaaatgcaat tagtgacggt atatcgtcgt aataatctcc gtttgaaatt tccgggcaaa
     2341 taaaaccaga gtttttttta aatacagggt gcatcttccc gttatcttcg atatacatac
     2401 cgctttcttc tcgttttttt tctttaattc ttaaatcctg actgtccgtt acagtgtcta
     2461 agcaaaatgg tctgaattcc atgattataa ttcactcctt taaaaatttt attatcataa
     2521 attactgatc cctccatgct tccaatgtac cgtttaatgc ctcgttctcc tcctccagtt
     2581 ctctcactct gtcctgcagt tctcttatct ttttattccg atattctatt tcctccttta
     2641 atctgtcaat ctctgaatcc attaataaat cccccctttc tggttcatcg taaaaatctt
     2701 tttttgcctg ttctcctaaa aatcttcttt tttcatattc ttcatattcg cttttctctt
     2761 taattgcaca taattcataa acctctctga attcctcctc tgtaatgggt tcaaatttac
     2821 catttaaaga ccagtgatct tttatttcaa ttttttcata gtctaaataa atatcccctg
     2881 tctttatttt tgaattaatt atccattttc taatagataa taataaattt tttattcttt
     2941 cgttcatatt atgccacctc tttttaatcg agcatttttt tgatttcttc tatcatttct
     3001 gttcctaaac tagtaggata tatatcatta ttttttcata agtaaaccct cactttctta
     3061 gtttttacac cgtaattaac agcttcttcg taagtgtcaa agaacacatc taatctgtac
     3121 cctttaatat ctcttccagt gtcggctgct atggcatggc catatcccgg tatatgcagt
     3181 ttagtccaaa agggaactat agcaggatcg acagctacaa ttccctcttt aggccatatg
     3241 cccgtcgcac aggtgttacc tgtatgagta taagcagtga tttcaaatgt tgcttcccag
     3301 taccgggatt taggttctgc attataggtt aacagtataa gcagtaatga tattaataac
     3361 agtcgtttaa acaatcttta tatcctcctt aattgtttgg gatcgaaccc ccatgcatct
     3421 tcgtctttat catcaaattt tactcttatg ccgagatact tacctgagtt ttcaataaca
     3481 acacctgttt gaaagtcaaa aaatctgaaa tctgcacagg gaacaataac ctgaactgct
     3541 gttcctacag ggaagtcgct gaatttatta tcccttgttt taaacctgtc gtaagcatat
     3601 tccctgagtt cagatatgga aggatttttc ttgcctggta tcttatgaac aagtctttcc
     3661 ttttgctttc tttttttatc ctctgctttt tccttctctt gttcttcgaa taatcttttc
     3721 tgttctatct cgtcaagtgc catgtaaaac atattactct ccaatctttg ttaattcgtc
     3781 aatcttcggg atagttgcct gttctatagt aagatgtaaa tctcggagta attcgtttcc
     3841 agttgatatt aaactataat aaggtcttat ggtgtccaga actttttcta taatataatc
     3901 tcttgttctg gtttcatggg tggggatact cattttaaga ttatcaagga attcctgcat
     3961 attggaagat ttttctaaca atcttgctgc gtcaaggaaa gagaaacagg atacttcccc
     4021 ttctttattt cttatttccc acataccgtt aaaaccaggc ttttccatgt aatactccat
     4081 acataccagt attgcataaa gaaaagctgt ctgctttaat gttctgatct ttctgtccag
     4141 ggtaatcttc ttgttgagtt tttttatctc tttgataaga gcttctttta aatgctccct
     4201 tgttttcact ttctattcct cctcttttgc cttataataa cagtggtaac agatgtatgt
     4261 tccgtcaaca gtaaaaactt ttctttctga tatttcttca ccgcatttta cacaggtaac
     4321 ttttttatct tccggcactc ctgccagttc tcgttttggt gttgcggttt cttttatagt
     4381 ggagaatttc tttctgtaac agtctgtgca gtaatctccc tcagatgtgg agaaaaatcc
     4441 tacttcggga tcttcgccgt aggtggagca ggttataccc tgtttagatg gtatatggag
     4501 atttttaagg cagtaatcta tattttcttt tacaagtttt tctattacag ggtaatcagc
     4561 ttcgttgaga tagaaacttt taccgttaaa atctataata caatgtatta tatcttcata
     4621 ttgcatcagg gtatctcttt ttgcgttcca ggttttattt tccgggatat aggttatggt
     4681 tccgttggtt aaaactaaaa ttcctatttc ctcggtttcc gtttctcttt cttcattatg
     4741 atcgtacacc ctgcagatag taaccttatt atctaccatg tcctcttccc tccttctttt
     4801 agatatactc tttctattgg tgttaatcca actccgttta tatcacttac atataactgc
     4861 ctgtaaggaa actgtgcatc ttttgttttt atactttcta ctacacagat tttttctgca
     4921 gttagatcgg aatatgccat accgagtttt aaattttcgg ttatttctct gtatttttca
     4981 ttcagagtct tgattttttc ttctatctcc ttttttaatt ttattatttc ttcctgctgt
     5041 tctttaaatg ttggcatcag tttctccctc ctttttaggt tctggacctt caaacattgt
     5101 aacaactgtt ttaattttgg ctccacatat ggagcaggta tttgttaccc ctttagagag
     5161 ttctctttct tctttaatgt cttcctctgt aagtggttcg ttacagtgtt cacaggttaa
     5221 gtcaacgaaa aacaggagtt taattttccc gtcaactgtt tcggctgata ttttcataat
     5281 attcctttcc gggagggctt gcaccctccc atagtttatt atttttcgta ttagagagtt
     5341 ctctttcttc tttaatgtct tcctctgtaa gtggttcgtt acagtgttca caggttaagt
     5401 caacgaaaac aatacctgtg tctttttgtc tttagataat aatattaccg tcatgatttt
     5461 attcttcctt tttccatatt ttttttactt ttattttttt tactctaatt tttccatctg
     5521 tagcaaatgg gattgctact atatcttttg ataaaaattc acattctaat attatatcct
     5581 cttcgtcttt attaattaaa caccactgta aagtagcaac gcttaatcct gcaccacatt
     5641 ctattctttc gtcagtttca tactcttcta caatgtaata tttttctact tgataagtga
     5701 ttttattatc tgaagtagga cttttaaaat cttttgtgac gtatttatat aacgtcaatt
     5761 tagtttttgg cggcatttgt ttgagacaat ataattgagg caaatggttt tttgcttcac
     5821 agaggtctgc tccacggagg tttgctccac agaggtttgc tccacagagg tttgctccac
     5881 ggaggtttgc ttcatagagg tttgctccac ggaggtctgc tccacggagg tctgcttcac
     5941 agaggtctgc tccacggagg tctgctccac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn tctgctccac ggaggtctgc ttcacagagg tctgctccac ggaggtctgc
     6121 tccacggagg tttgcttttt ctaaagtttc gactgttaaa attatttttc ctgtaaatct
     6181 gtttacaatg ttcataattt tttcctttct gggaggatat tataccctcc catgtttgaa
     6241 tttattcttt aactgctgtg gtagcagaca tgctaaccgg actgtcttta aaatcagaca
     6301 ttgttttttt aggcgtggac ttctcttctt cctgtcctgt aactttatcc aatgccctga
     6361 gactgctttc tatgaacttt gcaaccttct ttgcaggcat atttttacat ttcccatgtc
     6421 tctccagaac atttgcaata tcttctatga aagataccat acttcaactt cctcctttct
     6481 atttaacata atagcctcct atatattcat tttatactgg ccgtttctgt cttctattga
     6541 agatttgatt tctatatcca cttctgcccc taagatatct gccagttctg caatctgtga
     6601 atttggtata ttgacggcct tgattgtaat agtagtaata cattcatcat ctttattcgg
     6661 tcctttcggt gtgaattttt ctactatgca atttttgatt atcaatgtat tccctccttt
     6721 cttattttca tctaaatact gctttaatcc ttcatcggta agtttttcta cctgctccga
     6781 ggttattgtc catgtaggca tagtttatat ctcctgtata actattttcc cgttcttaaa
     6841 tccaacatta aacgcctgtg tcttgccttt gcctttagca aaatctatcc cgaaaaatac
     6901 gtaatctttc atttcctgag ttttgcctga attccttgat ttttcttcgt gtttaaatct
     6961 atccttacat ctcttttcaa gatcttcctg caataaatct gcaatagtct ttgtgtcttc
     7021 ttttacttta gtacaggcct gtactttctc tcccatgatc ggtggagcct tgacttcagc
     7081 tttaaaagcc cgtgcatttt cctggatctc ctttatcatt ttctggactc tgggaatatc
     7141 tttacctttc agcttgtcaa tttcctcgaa gtactcaaac aggctgttta tcataagggc
     7201 ctgttttttc tctttgtcta cggtgatgcc ataggttttc tctgtttcca tagggaaaag
     7261 aactgatata tgttttataa acccctgaat ttcaagttcg tttaccgaaa gggctatgct
     7321 tttaaagagt tttcctatgg taaagaggag ttcttttttc attgtatttt tccagttttt
     7381 aagttcctct tccttcttct ttttatcttc tttcggtttt tcgataataa tatctattga
     7441 cctttttaat ctggatatgc cttctctttc acagggttcc atttcttcat tcaatattac
     7501 ccttgttcct ttcctcccta aactttttcc gtttattctt atatcttgga cagggatttt
     7561 tccattacta taagaatccc caaagtgtgt cgtatatatt ctttcatatt ctttcatatt
     7621 ctttctcctt ccttaaaacg gcacatcacc gtcaaacata tcctgcattt tttcacttgt
     7681 cgggatagtg ttatctctta tatcctgttt ttctgcttcg ttttcctctt cagttactcc
     7741 tccgggaaac tggccgttct ccattttatg tttctctgcc agttcctttt tgagatggtc
     7801 aatgagtagt cctgctttta taaaatccat atcctttacg gattttacat aagtctcacc
     7861 ggtttcatac cggtattttt cgaagaatct ccctttcttc tgccagtcat attccagttg
     7921 tttgaaaagt tcgtctgcct ctgctatctg agaatatgaa gcagggacat gagcatgtct
     7981 ttcctgcggt gcaattatcg gagcttcctc cggtgcaggt gtcggaggtg gaatttccat
     8041 tgctttcctg tcagcttcta ttactttctt tttaaggaga gaaataaatt tttccgcgtc
     8101 ttcctgggaa aaatctgctg cagtttttaa agtagtgaat ctatcagggt tcttctctct
     8161 catataggca aaatattttt cctgtttctc ggtatcccat tttgcttcta tatagagatt
     8221 gtttatctga ttaagttgat ctggggttat tcccggtgac ggcggtggaa gtggaggttc
     8281 ctctgttatg gtataatctc cgtcaataat atctccttta cattctggcg gtggtggaat
     8341 ttctcccttt tttatagttt caagggtatt atttacttta agataatcag cgtaagtcat
     8401 gccttttgta ctttctatct ttctgccgag acagtttgat acaaaggaaa ttcttcctga
     8461 tctgtcttcc tctttatatc ctgcatcttt caggagtacc cacattcttt ttctttgggc
     8521 ctctatgagt tcttttgtat ctatctctgg agtcgtagga agaaattcat cacagtcggc
     8581 agcagaaaaa ccgcatccgg tagctttttg caatactctt ttttctgctc tggtttctgc
     8641 aagttctaag gggtgttttt gccctttttt tacattatca ggggtggcag tgccataggc
     8701 ggtaaatact ttttctttgc cattagcaac ttctatcctg agttcagatt tacagattat
     8761 aactctgtct tcttttttaa atcccaggca atttgtttta ataaagtcgt attcttcccc
     8821 cattaaaaca tctgtcttga gatcatactt tcccctgtaa agttggtcca ttttccagtt
     8881 aagcccggcc ttgtcgatga ccaatttgaa ttttccggtc tttggattaa tctgcatatg
     8941 aaacatttgg tcaatcattt caaccggcat tgcaggctca atattcctca cggctaatgt
     9001 tgctgcttta tccagaggaa ccagggcgta agcagatcct tcttcgctgt aattatatgc
     9061 catatctatc actccttttt ttcaaatttc tctctaaggt aggcagagag tttttgtcct
     9121 ttttcctttg cttcttttat aagggcatct ctttctacct ccgagagcct tatagttaca
     9181 ctgcttgttt ttttattttt cttttttaca gtcaaataat caccccctct tccggtaaaa
     9241 taaataataa taaacaggag atgtttcact cctgcttatt gattttagaa tggtatttct
     9301 attgactcaa actttatctc tccgtcaatt tccatccatt ttaaatcgta tttttttagg
     9361 gcctcagtta ccttcttttt atggtccatt atcaggttaa aatcaccggg ttcaaaatcc
     9421 accgggtccc catctatttc aatattgtaa tcctgccagt ggagtctttt gatttcggga
     9481 taatacagaa ctgtctggca gcctatttga atttctttgt attccggcaa ttttttatcc
     9541 ctgatacata tttttatata attatctgca atatctacca ggggcatagc tccgtgttct
     9601 gttttataca gatggattac ttcacacatt attttttcat tcagggtagc aatcatcttt
     9661 tccagttctt cttctccgaa cccattgaag aagtaccggt cgaaccgtgt ccagaaacct
     9721 tgattttcca gacatattac attgtctttt gtacagaagt aaacctggaa gtgttctggt
     9781 ttctcgaatt tattcgggat aaagaaacac ggtacatctg gattattatg tctttcacaa
     9841 actctgcctt ctttatctat gaaaaaccag tcattattta gcatgttaag gaaaatccat
     9901 ctatggggtt tttctctttc tccgtcttct ataatataag atataatatc tctttcttta
     9961 agggcattta taatatcact ctggttatag aagaaatcat ctcgaagtaa agaacagtgg
    10021 ttccattcgt gaggaatgtt tgtattaatt ttaaggtatt cactgttatt atttgaaagg
    10081 ataaaaaagg agttatccgg tgctactttt attttgacat aagatgtatc taccggcagt
    10141 tcaattccct tttcttgaac ttcctcaaaa tccctcgttt cataacattc acggtagtgg
    10201 ccccaccatc tttcaacttg ctcagtgata agtccttcgt ctttttcgga tactgatttt
    10261 tcgaaatatt caagatacac ttgaaggact gaccaaaagg tatcccaggt gatagttaac
    10321 ccggaattta ctttgtcgtt cattgaggag taaacaaggg gtattctctt gacgggtgtt
    10381 ttgtattcct gaattatgtc ggttccagga attatatcgt accggatatg tcctccagtc
    10441 tgagcgtaaa aatcacggca gccggaatat ccgatgtggg attttgtttt gtaaaattcg
    10501 ggaggaggta tgttgatttt atctgtttct gcattatagg caataaagga gaacatcagg
    10561 tcaagggctt cttttgtttc caggcaagcc cctatttgga catagcttac cttccattcc
    10621 cctttttcgc cacattccag actccacagg tgacggctgt gtttttccct gaagtatgtt
    10681 ttttgcattt taatttgccc ctttcttgtt ttatttttgt cttacaaaat tcattataaa
    10741 ttgtcttaca ttttttgtca atatgtaaga caaaatattt taaataaaaa aacgcgtttt
    10801 aagcgtttta aagttaaaaa atatatattt ctatatcggg ctgaaacttt ttaatcctgg
    10861 tgcaaattac ccggcaaatt ttgcatttta cgtgaagagt ttacccgtga atttttaccc
    10921 tccatcagtt ttgctatttc tgccgggaat tgtttgtatt cttttcctcc aagaaaggcg
    10981 tttttctctt ttccacccca ctgtttaaag aaaaatggta tgttttcata ttctgtaaag
    11041 tctctaagca cacttacata ggcaggttgt aaaactcttg catttattcc tgtttcaccc
    11101 ccaactataa tccaatctac tttgtaataa ttgctattta gtactgatac tcccggtata
    11161 aggggactgc cgataagtgg ctcaaatgag ataaacttgt gttttgccgg tatcttctgt
    11221 aacgtctctg ctcggtgaag gtatttactc gattcgattg ttactccaag ccagatgtta
    11281 tctgtccaat aagattctct tttataatat tcccattggt aaggtttttc atttttccaa
    11341 ataggaatat tttctggacg ttttgttaaa ataagaaaaa tatgttgtgg gttatcaacc
    11401 atctctcgaa atatatcgaa caaatattgt ttctttaaat cctcatgaaa caggtcactc
    11461 atactattca caaaaaatac agtcggtttc tttgtcttcg atggaatgtt taaatgatgt
    11521 atacaaaatc tcaccacact aaaaggctca ctgtatttct ccaggcccat tgcctgtaat
    11581 cttttgtgca ttttttctgc atagcagtta aggcannnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnaactg agagaagaag tcgtaaggaa ccagatcata atatccgttc
    11761 ctggggagat ataacatttt cctccggcag gtctgacact ggacaacttc cttttcaaag
    11821 gaactctcct ccagggtgtc caaaccgtat tccctgcagt aaaggcagtt ttgtctcata
    11881 agatacttct ttatgttttc aatatctttt tccgttattc tcagatgagt atccttcctg
    11941 ataactgccc tgagatattg gtttatgctc agtcctgctt tctgagcttc tttccttaat
    12001 atctgatcat cttcggttgt cataagaata cttcttgaga ttccatattt cttttccatt
    12061 ggcataaaaa tcactcctta aattttgtga agtgtattat atcataaata aatattaaaa
    12121 tcaatattga caaaaaatta ataaatatgc taatatgagt tatattttga aaggggtgag
    12181 atatttgaat tgtgatttta ttccttataa atatgactca gatccagacg aggaatgtta
    12241 cctatggagt aaatatgttt ttgcggtaaa atatggatat gtctgtccat taccggaaga
    12301 acaaagattt ctattagatc atatagatat aattaataga aatctccaga aatataaatt
    12361 atatcttcat ccatcacctg acggaattaa cggaggagta tatacaagtt actccttcgt
    12421 attaaggagg atagaaaatg tttcaactgc caattaatga atttacgaca ggagatgcta
    12481 tagatggagt aaagcatatt ccagaaggag aaattgattt aattttctgc gatattccgt
    12541 acaatatctc aaatgaaacc aaaatcttca gagattatcg tcaggccaat ggaaagaaaa
    12601 aatctgccaa tatctctttt aacttcgatg aacaagagga aggagaagag gaagataaat
    12661 gggatcacga ttttgaccct ataccttttt tagatgcctc tattccctgt ttacattcta
    12721 ctggatcatg gattatctgg tgtgctgaac agcaacatgg acacatcaga ga
//
LOCUS       DAOQWT010000003        11723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_5943_length_11723_cov_5.220860, whole genome shotgun sequence.
ACCESSION   DAOQWT010000003 DAOQWT010000000
VERSION     DAOQWT010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 11723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11723
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5943_length_11723_cov_5.220860"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3578)
                     /locus_tag="PKJ95_00190"
     CDS             complement(<1..3578)
                     /locus_tag="PKJ95_00190"
                     /inference="COORDINATES: protein motif:HMM:NF021648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HNR64692.1"
                     /translation="MAGDPTGTVFLAGKIQVGLESGNTQQNLSNIIKSVKEEISVFNQ
                     SNKLIIDVDVQNAMRIRSLANNIRDIKSAIDSLSNVKVIWENMSTHMKSAFATANNLA
                     VLAKDLPKVFEPLKESLMLGREVRSSIMDYADALDRIKANAGLLTTLKSKLPTVLSSV
                     NTPSVVPGVVPAGTPGGAGAGSKAAQEYRDTVEKLHAALVNVFKDINNLDRNEFWKKN
                     KEVIIQSFNALQKYHEETGNVFAQGQDYVKLIQMINKEGIKPSKGLMEEEKYLQGIQL
                     LRKNGIITEAEYNGFILKRQEALKKEQDASRNALSLRAEMLRNAKRESDQEIKNVEIQ
                     HRKTVEYERQKFAKEAKRLGNTGDIADNQLAQAAIINRINNLRNSTDLADKREIINLN
                     KILDQLTKQEVLLKNKISLTTRENELLAMMDRYLNGGTKEIKNQAGAELSKLKVSEKE
                     LLLKTAKNKLDSEHYDQLKKQLGYRRGITELFFDEMRNWPLMIARQAAYGAQWTLIFG
                     IIHRVQAAIREGLRLYGEFERGTSRALRTGLTPTQLAGAFTPQANGGMKEAVTHYREL
                     LNIQAMIFTSRHKASVKEYMDVIYELTSANMSLSKAMQFADTTMKVAIAAEGDMTQTA
                     RTMAGLYNIYGKTIKGVGSEQEKFNQIANTVLVTWSREQIELTELNNALKYVAASGRA
                     IDLDYRVLVTTIGHLNTQMIRGATAGTGLRQMMSQVSKDAGGLKNAFGDFTNDLIAFS
                     KQEKMSFDPQKPMDFIAVMEAMRKKLLANRKDFVDYGQDIVYTQKELSEVYKIMSLRG
                     ANVGLTLLRTLPEFKKKMQETLGLTKDFADNFIKIQEMNIPVQWETMLKNLAMLPAAF
                     LMGATHTESIAQAMANINASMTNVIVGAFAVGKTISGWGDYILQATLKLDSLMNLLHE
                     HSITTALSNVPVIGSLFGEEGIFGTGITSGRILPDLYARGKGLINKTKAEVNDPKKFM
                     KDLGLRVDELGKYFGFTTNAQNNAEKTLDMSSKKFMENLNQRIEAANTESLTWINIGD
                     YIRGTADSLERYTQIYKELNGAYDIGQMGKVSGEFKKQQTYIEALNKTMEGQINFYSK
                     MKETNKAQRENPGELMQVINSNIQMREKNFGGENAEKRINKVKELINEYNKLNQFALP
                     VAATGNIRGKMQNAILAPEFKKREDEIQELKRQHDRVSNDIINERK"
     gene            complement(3579..3965)
                     /locus_tag="PKJ95_00195"
     CDS             complement(3579..3965)
                     /locus_tag="PKJ95_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64693.1"
                     /translation="MLLHILGLKVNDIQELDYWQFYGYQKVLNELVKIYYLFEGHIKR
                     ITRLLFVISTSPWIGLKDTSIEKFFEFEELLQASSGEKKEKIDPLEGKGEWIEKKDGT
                     KEWVYNYEGLQDIRNFLDKFNGRRGI"
     gene            complement(3995..4531)
                     /locus_tag="PKJ95_00200"
     CDS             complement(3995..4531)
                     /locus_tag="PKJ95_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64694.1"
                     /translation="MEKGLNEYTQAKDISFQKMLGMGGVEFTEFQMKDGVRITVTHFA
                     MPITIKNLKKLEALKEQIDEIRYNFSTEAEVILKKMTEAQKKFTEDMPAEISEKITKE
                     INELEKEYKKKEKKYKTAILDKKLDVAVLCFCRTEETTKEEISEWIDMQSLERICEIA
                     DGMSYPPKRLPEPEEKEM"
     gene            complement(4591..5337)
                     /locus_tag="PKJ95_00205"
     CDS             complement(4591..5337)
                     /locus_tag="PKJ95_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64695.1"
                     /translation="MQKILRGVGTFRAKIPFGTSELWVPFITAKEINLGISAEDARQT
                     GGDRYFPLDIMTTSKTGTVRVTDAALHLDIFKLLGTGDPGSTENFQVKEILTVSAGGV
                     ITTGASYVVGTVSLMDQYENFVSSGYVATDGATITGLSSYATTKMAVIYETADVSDAV
                     VYSIHVNDLPTYFECVHVSRYRDPADNSIKLFQTRIYSCRPKGNLEYSYRHGEFSAPS
                     FEAEVLDPGRVDGAVLTYAFGTEPAGLTTS"
     gene            complement(5353..5826)
                     /locus_tag="PKJ95_00210"
     CDS             complement(5353..5826)
                     /locus_tag="PKJ95_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64696.1"
                     /translation="MIMKPNYIFSAIWKVISNNEDILKYLGMDISTNKKIVRQRILPN
                     LPTEYNNLPVITVLGRVNGRESPSMPFMDEIQFQIHALTYDDSYAENIGILEQIRELI
                     DNETLPLEEDSGIAYWSGFRWLNEGKVPDISPDVYNYYHTYQTELYRSFSKRSGM"
     gene            complement(5829..6764)
                     /locus_tag="PKJ95_00215"
     CDS             complement(5829..6764)
                     /locus_tag="PKJ95_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64697.1"
                     /translation="MLGIGQNSISENKRDTSDKLFYYRHHRTKSFADLGRMGGSIPNY
                     FSVELDVSGFFRNIREAFLIELEALQQEYITFVSGVYRNAQFISFPGTPKIITATMQK
                     VIGAFIAKVADVKKFYLAYVEHGTGVNAMAGWTPDMMDSVIGAERGIQRPADGRIVPR
                     GKGYEWTDPGGETHPGANFLFIPQQKGVKPNRDKESRKGDDRSQFKIRPWSMGQVGQG
                     LLNQKNFAFLTKEKLYIFAQKVYDRALSMPGSFKQGGKMERLTATRYKQFTEKVGKEQ
                     VSSGLGQFRLKATSERYEQFKKNQGSRPGFVDGDY"
     gene            complement(6767..7390)
                     /locus_tag="PKJ95_00220"
     CDS             complement(6767..7390)
                     /locus_tag="PKJ95_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64698.1"
                     /translation="MKSLKRLLARELNHYSALCQIFDLEDKFVKEEHIQISPVQRSID
                     ENISAFGRTAVFPYNTVVKSGYIIKNTAENEEYFISSWRAVSLCSSPAAFSAFLLQIN
                     ATGDIYRNEELDFNPDTGEVKTGYVKKISGLKLYVQKKDWTMEMMTAYGREQKGYETI
                     MCQERDIRQGDRIQCPDKKYIVQDINRIIRDNMLSLQVGVNIKHIEE"
     gene            complement(7392..7742)
                     /locus_tag="PKJ95_00225"
     CDS             complement(7392..7742)
                     /locus_tag="PKJ95_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64699.1"
                     /translation="MEQSMVIDLTVSVSESTKSSTVYTTRVSSTIDANNTFYIPVPDL
                     TTDKEVDLATLLGSAAEYVIIDSPERISVKTNLNTNDAQVGKLIVLSGTSVSKVFITN
                     ASGEDIDVKIIAGG"
     gene            complement(7754..8368)
                     /locus_tag="PKJ95_00230"
     CDS             complement(7754..8368)
                     /locus_tag="PKJ95_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64700.1"
                     /translation="MIYNKDFKRNYQFSEYVSVEDFKSYPLSLDIELTDAQIKFILDN
                     AGYVIQKVITRPILLTPVIDRVTGNDNYNIMVRYPINALNKLLENYGTSSEEVADEYI
                     KLSSISVDTSPLYSNNIEKLNSVWDSECIYEFQYTTGSITFPSEVKMATILTAASIIN
                     NQERKGIKSVKIADLQVNYSQEFDIIPPEALSLLSDWITKQYVV"
     gene            complement(8365..9393)
                     /locus_tag="PKJ95_00235"
     CDS             complement(8365..9393)
                     /locus_tag="PKJ95_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR64701.1"
                     /translation="MKYILNPYKILNHVPVLNEMIKDNKTPPMAVEIDLTNLCNHNCI
                     WCMFDRYRAKNPVSLEKDLVFNLLQDLKAIGVKAITYVGGGEPLLYPHFKEVLYKTKE
                     LGFDCGVVTNGELLIENIQAIKDCCQFCRVSLDAGSSADHKALHRGRWNSFKRVLQGI
                     EALGKEKGDLILGVAFLLHTINFYKLTELVKILSILKVDYLQVRPVYMPGLVFTDRVK
                     QKINIQIDEAQKMAHTTGLHLITFKHRFREFYEEDLRPEKCLAHNILSIIGADSNVYL
                     CCQLRGNPKFSIGNLKEKSFKDIWESDQRQEVIDRIDLSRCPPCRYKKYNEIMEYLSS
                     ERRHANFL"
     gene            complement(9377..10246)
                     /locus_tag="PKJ95_00240"
     CDS             complement(9377..10246)
                     /locus_tag="PKJ95_00240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR64702.1"
                     /translation="MRTVYLIILNCNNMSVIPELCHSLKEALKNNEYPVDIRFIDQGS
                     TDGSYEFLEEFCSLFNNCVSCTVRRLKKNVGTTKGWNIALKDICNSHTNYVCLINSDM
                     KVSKNFLKPMIDIMENNPKCGMVSNTLRDYTNINFVQNYGPDMSNPWHYRMGIMPVEL
                     FNNVKPFQAEWGHMGCTLFRSEVFEDIGLFDENFFIYSCDFDTQFRMKLAGYEIWFCP
                     QSYAQHKTFVTCNELKKNPVIEELCKKDGEYLHWKWGDKVFEWFNYTLTHDVNKNLKA
                     VYGEDFKINEIYS"
     gene            complement(10243..11358)
                     /locus_tag="PKJ95_00245"
     CDS             complement(10243..11358)
                     /locus_tag="PKJ95_00245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNR64703.1"
                     /translation="MVQFYNHFLSLKDQDIKKVEYLRNSIKQMGDKCSFKEITPKKIV
                     GDGKLNILVQRLGGLGDILFCTPIIKYLHSLGHNINFFLHFGYWEALVNNPYIDKIYC
                     LENGLKQENRPPVNGVLPLSITPEIFSSHDKTCVFQGAIEHNKQAEIDHVIDAYYKWV
                     DIDPEGKPKDLIINLTEEEKAWGYKDLIAMGYNPNKPFVSVSPDASFAKNRSWPIPYF
                     KKLRDLLKDDFNVVYIHRDEGRTPQRPKGWSLRQMFSIVYNMDLCVTVDTGVLHVAGA
                     FQKNIIALFGSFNPDLRVRYYKNCEVICKNPGLCGKFCFSHGDQCEYVFNKLSLKQRT
                     VKTNWQGIYPPCLTAIKPDEVAKKVRERINTERRVAA"
     gene            complement(11471..>11723)
                     /locus_tag="PKJ95_00250"
     CDS             complement(11471..>11723)
                     /locus_tag="PKJ95_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64704.1"
                     /translation="DVSSYNNGYFTCNIDIEYDLPTTEDAAVSCMVDHTDMKRAELIP
                     ITAEPLAKIDNTVERMITCEMTFEYGTPVSSGQIVGLNE"
BASE COUNT         3254 a         2438 c         1857 g         4174 t
ORIGIN      
        1 ctttttcttt cgttaataat atcattagat actctatcat gttgcctttt taattcttga
       61 atttcatctt ctcgtttttt aaattcagga gcaagaatag cattttgcat tttacccctt
      121 atatttccgg ttgctgctac aggtaaggcg aactgattta atttattata ttcatttatt
      181 aattctttta ctttattaat tcttttctct gcattttctc ctccaaaatt tttctctctc
      241 atttgaatat tggaattaat aacttgcatt aactcaccag gattttctct ctgagctttg
      301 ttagtttcct tcattttact gtagaagtta atttgtcctt ccatagtttt attgagagct
      361 tctatataag tctgctgttt cttaaattcc ccggatactt tacccatctg ccctatatca
      421 taagccccgt ttaattcttt atatatctgg gtatatcttt caaggctatc ggcagttccc
      481 cgtatataat ctcctatatt tatccatgta aggctttctg tattggccgc ctctattctc
      541 tggtttaaat tttccataaa tttcttactg ctcatatcaa gagttttttc agcgttattc
      601 tgggcatttg tagtaaatcc aaaatatttc ccgagttcat caactcttaa accaagatct
      661 ttcataaatt ttttcggatc atttacttca gcttttgttt tatttattaa acctttacct
      721 ctggcataca agtcgggtaa aattcttcca gatgtaattc ctgtgccaaa aattccttct
      781 tctccgaata agctacctat aacaggaacg ttactgagtg cagtagtaat tgaatgttcg
      841 tgtaatagat tcatgaggga atcaagtttc aaggtagcct gaagtatata atctccccat
      901 cctgaaatag ttttacccac agcaaatgcc ccgactatta cattggtcat agaagcattt
      961 atatttgcca ttgcctgcgc aatgctttct gtatgagttg cacccataag aaaagctgcc
     1021 ggtaacatag caaggttttt aagcatagtt tcccactgaa caggaatatt catttcctga
     1081 attttaatga agttatcggc gaaatctttt gtaagtccca aagtctcttg cattttcttt
     1141 ttaaattcag gtaaggttcg aagtaaagtc aagcctacat ttgcgcctct taaagacata
     1201 atcttatata cctccgagag ttctttctga gtatatacta tatcctgccc gtaatcgaca
     1261 aaatcttttc tattggcaag aagtttcttt ctcattgctt ccatgacggc gataaaatcc
     1321 attggtttct gagggtcaaa agacattttt tcctgtttgg aaaaggcaat taaatcattg
     1381 gtaaaatcac caaaggcgtt tttaagccca ccggcatctt tacttacctg actcatcatc
     1441 tgacgaaggc cagtgcctgc cgtcgcaccc ctgatcatct gagtgtttaa atgccctata
     1501 gttgtaacga gaaccctgta atcaaggtct atggctctac cggaggctgc cacatatttt
     1561 aaagcgttat ttaattcagt caattcaatc tgctctcttg accatgtaac caggacagta
     1621 tttgcaatct gattaaattt ctcctgttca gaaccgacac ctttaatagt cttaccatat
     1681 atattataaa gacctgccat tgttcgggct gtctgcgtca tgtcaccttc tgcagctatg
     1741 gcaaccttca tggtagtatc tgcaaattgc atagcctttg acaggctcat gttcgcagaa
     1801 gttaactcat agataacatc catatattct tttacacttg ctttatgccg ggaggtaaat
     1861 atcattgcct gaatgttaag taattccctg taatgggtaa cagcttcttt catacccccg
     1921 tttgcctgtg gagtaaatgc cccggcaagc tgtgtaggcg ttaagcctgt ccttaatgcc
     1981 ctggaagtgc ctctttcgaa ttccccatat aacctcagac cttctcttat agctgcctga
     2041 actctgtgga taattccaaa tattaaagtc cactgtgcgc cataggcagc ttgccgggca
     2101 atcattaaag gccagttccg catttcatca aagaacagtt cagttatacc tcttctgtaa
     2161 cctaactgct ttttaagctg atcgtaatgc tcgctgtcta atttattttt ggctgttttc
     2221 aggagaagtt ctttttctga tacttttaat ttacttaact ctgcgcctgc ctgattttta
     2281 atttcttttg ttccaccatt aaggtaacga tccatcattg caaggagttc gttttccctt
     2341 gtggtgaggg aaattttatt tttcaataaa acttcctgct tcgttaattg gtctaatatt
     2401 ttattaagat taattatctc tcttttatct gcaaggtctg tagagttcct taaattatta
     2461 attctattta ttattgccgc ctgtgcaagt tgattatcgg caatatctcc agtatttcca
     2521 agtcttttag cttcctttgc gaacttctgg cgttcatatt caacggtttt tctgtgttga
     2581 atttcaacgt tttttatctc ctgatccgat tctctttttg catttcgaag catttcagca
     2641 cgaagagaaa gagcatttct actggcatcc tgttctttct ttaaagcttc ctgccttttc
     2701 aggataaatc cgttatattc tgcttccgtt ataatcccat tttttcttaa aagctgtata
     2761 ccctggagat atttttcttc ttccattaat cctttagaag gttttatacc ttctttgttt
     2821 atcatctgaa ttaatttcac ataatcctga ccctgtgcaa aaacattccc tgtttcttca
     2881 tggtattttt gcagagcatt aaaagactga ataataactt ctttattttt cttccagaat
     2941 tcatttctgt caaggttatt tatatctttg aatacattta caagggcagc atgtaatttt
     3001 tcaacggtgt ctctatattc ctgggctgct ttactgcctg caccggctcc tccgggagta
     3061 cctgcaggga caactcccgg aacaacagat ggggtattca ctgaagagag aaccgtagga
     3121 agtttacttt ttaaagtggt aaggagtcct gcatttgctt taatacggtc taaggcatct
     3181 gcataatcca taatactgga acgaacttca cggcctagca ttaaagactc ttttaatggt
     3241 tcgaaaactt taggtaaatc tttagcaaga actgctaaat tgttagccgt tgcaaacgca
     3301 cttttcatgt gcgtactcat attctcccag ataaccttaa catttgacag ggaatctatg
     3361 gcagatttaa tatcacgaat attatttgca agggatctta tcctcatagc gttttgaaca
     3421 tcgacatcaa taataagctt gttactctgg ttaaatacac ttatctcttc tttaaccgat
     3481 ttaattatat tgcttaaatt ctgttgagtg ttgccggact caagccctac ctgaatttta
     3541 cctgctaaaa aaactgtccc tgtgggatct cctgccattt atattcccct cctgccatta
     3601 aacttatcca agaaattcct tatatcctga agaccttcgt aattataaac ccattctttc
     3661 gtgccgtctt tcttttctat ccactcgcct ttaccttcaa gcggatctat tttctctttc
     3721 ttttctcctg atgatgcctg taataattct tcaaactcaa agaatttttc tatactagta
     3781 tcttttaagc ctatccaggg agatgtagat attacaaata ataaacgggt tatccgtttt
     3841 atatgcccct caaagagata atatattttg actaactcat ttaagacctt ctgatacccg
     3901 taaaactgcc agtaatctaa ctcctgtata tcgtttacct ttaatccaag tatgtgaaga
     3961 agcaataagg taaaccaatg agatacttta ttctttacat ctccttctcc tccggttccg
     4021 gtaatcgctt tggggggtaa ctcatcccgt ctgcaatctc acatattctt tcaaggctct
     4081 gcatatcaat ccattctgaa atttcttctt tagttgtttc ctctgttcta cagaaacaga
     4141 gaacagcaac gtcaagcttt ttatctaaga tagctgtttt atatttcttt tcttttttct
     4201 tatattcttt ttccagttca tttatttctt ttgttatttt ttcggaaatt tcagcaggca
     4261 tatcttctgt aaatttcttc tgagcctctg tcattttctt taagattact tctgcttctg
     4321 tgctgaagtt ataacggatt tcgtcaatct gttctttaag agcttccagt ttcttgagat
     4381 ttttgattgt aataggcatt gcaaaatgag taacggtaat tcttacaccg tctttcatct
     4441 ggaattctgt aaattctacg cctcccatac cgagcatttt ttgaaaactt atgtcttttg
     4501 cctgggtgta ttcatttaaa cccttttcca ttaataattc ctcctatttt attttttgag
     4561 ggagagagga taacccctct ccctgatagg ttaactcgta gtaagtcctg ccggttctgt
     4621 tccgaaggcg taagttaaaa ctgcgccatc tacacggcca gggtcaagaa cttctgcctc
     4681 aaaactcgga gcagaaaatt ctccatgacg gtaagaatat tccagatttc ctttaggtct
     4741 gcaggagtaa attcttgtct ggaataattt aatgctgtta tctgcaggat ctctatagcg
     4801 acttacatgg acacactcaa agtatgtcgg taaatcattt acatggattg aatatactac
     4861 agcgtcactt acatcggcag tttcatagat aactgccatt ttggtagttg cataagaact
     4921 caatcctgta atagttgctc cgtcagttgc cacatatcca ctggacacaa aattttcata
     4981 ttgatccata agggatacgg ttccaacgac ataagaagct cctgtggtaa taaccccacc
     5041 tgcacttaca gtaagaattt cttttacctg gaaattctct gtagagccag gatcacctgt
     5101 gccgaggagc ttaaatatat ctaaatgaag tgccgcatct gtaactctga cggtacctgt
     5161 tttagatgtg gtcataatat ccagtgggaa atatctgtca ccaccggtct ggcgtgcatc
     5221 ttctgcagaa ataccgagat ttatttcttt tgcagtgatg aatggaaccc ataattcaga
     5281 agttccgaaa ggaatttttg ctctgaaagt tccaacaccg cgaagaattt tttgcataat
     5341 ctatctcctt tcttacattc ccgacctctt actaaaagac ctgtagagtt cggtttgata
     5401 ggtatgataa taattgtata cgtcaggaga aatatctgga actttaccct catttaacca
     5461 tcgaaaaccc gaccaataag ctataccgct atcttcttcc aatgggagag tttcattgtc
     5521 gattaattcc ctgatctgct ctaaaatgcc aatattttcg gcatacgaat catcgtaagt
     5581 aagagcatgt atttgaaatt gaatttcatc cataaatggc atagaagggc tttcccttcc
     5641 gtttactctt ccaagaacag ttataacagg aagattatta tattcagtgg gaagatttgg
     5701 taatatcctc tgtctgacta tttttttatt tgtacttata tccataccga gatattttaa
     5761 tatatcttca ttattggata ttaccttcca tatagcactg aatatataat tcggtttcat
     5821 tatcataatt aataatctcc atcaacaaat ccaggtctac ttccctgatt tttcttaaac
     5881 tgttcgtatc tttcactcgt cgctttaagt ctgaattgtc caagccctga agatacctgt
     5941 tctttaccta ctttttcggt aaactgctta tatctagtgg cggtcagtct ttccatttta
     6001 ccaccctgtt taaagcttcc aggcatactt aaagctctgt catatacttt ctgagcaaat
     6061 atataaagct tttcttttgt gagaaaagcg aaatttttct gatttaataa gccttgtcct
     6121 acctgaccca tagaccaggg ccttatttta aattgacttc tatcgtcacc tttccgactt
     6181 tctttatctc tgttcggttt aactcccttc tgttgtggta tgaaaaggaa gtttgccccc
     6241 ggatgtgttt ctcctccggg atctgtccat tcataccctt tacctctggg aacaatcctg
     6301 ccgtctgccg gtctttgaat tcctctttct gcacctataa cagaatccat catgtcggga
     6361 gtccatccgg ccatagcgtt aactcctgtg ccatgttcga cataggcaag gtaaaatttc
     6421 tttacatctg caacctttgc aataaatgct ccgataactt tctgcattgt agcagtaata
     6481 attttaggag ttcctggaaa agatataaac tgagcgtttc tatatacacc ggaaacaaaa
     6541 gttatatact cctgttgtaa agcttccagt tctattaaaa aagcttctct tatatttcta
     6601 aaaaatccac tgacatctaa ttctacagag aaataatttg ggatagaacc acccatacga
     6661 ccaagatcag caaagctttt tgttctatgg tgacggtaat aaaacaattt atcgcttgta
     6721 tcgcgtttat tttctgatat tgaattctgg cctattccaa gcatgattat tcctctatat
     6781 gtttgatgtt tacgcctacc tgcagagaca acatattatc tcttataatt ctgtttatat
     6841 cctggactat atatttttta tcaggacatt gtattctatc accctgtctt atatctcgtt
     6901 cctgacacat gatagtttca tatccttttt gctctctacc gtaggcggtc atcatttcca
     6961 ttgtccaatc ttttttctgg acataaagtt taagaccact tatttttttt acgtacccgg
     7021 ttttaacctc tcctgtatca ggattaaagt ctaattcctc attccggtat atatctccgg
     7081 tagcgtttat ctgtaaaaga aaagcggaaa aagctgcagg ggaactgcat aaagaaactg
     7141 ctcgccagga tgatataaaa tactcttcat tttctgcagt atttttgata atatatcctg
     7201 acttaaccac cgtattgtaa gggaatacgg cagttctgcc aaatgctgat atattttcat
     7261 ctattgacct ctggacagga gaaatctgta tatgttcctc tttaacaaat ttatcctcaa
     7321 ggtcgaatat ctgacataat gcagaataat gatttaattc tctggcaagc aatctcttta
     7381 aagatttcac attaacctcc tgcgataatc tttacgtcta tatcctcacc ggaggcgttt
     7441 gttataaata ctttactgac agatgtgccg gacaggacaa taagtttacc gacttgagcg
     7501 tcatttgtgt ttaaatttgt ctttacggat attctttccg gcgagtctat aattacatat
     7561 tctgcagcac tgcctaaaag agtagcgagg tctacctctt tatctgttgt cagatccggc
     7621 acagggatat aaaaagtatt atttgcgtct attgtacttg aaactctcgt agtatataca
     7681 gtagagcttt ttgtactttc agaaaccgat actgttaaat ctattaccat tgactgttcc
     7741 atattaaatc ctcctataca acatattgtt ttgttatcca atcggataac agggacaggg
     7801 cttcaggcgg aattatatcg aattcctgtg aataattaac ctgcaaatct gcaatcttta
     7861 cagattttat tcctttacgt tcctgattat ttattatact ggcagccgtt aaaattgttg
     7921 ccattttaac ttcagatggg aaagtaatac tccctgtggt atactgaaac tcataaatac
     7981 attcgctatc ccagacagaa tttaatttct caatattatt tgaatacaaa ggagaagtat
     8041 ctacagatat actgcttaac tttatatatt catccgcaac ctcttcactg gaagttccat
     8101 aattctcaag caacttattt aaggcgttta ttggatacct taccatgatg ttataattgt
     8161 cattgcctgt taccctgtct ataaccggag ttaaaagtat gggcctggta atcaccttct
     8221 gaattacata tcctgcatta tcgagaataa attttatctg agcatctgtt aattcaatat
     8281 ccagggataa gggatagctc ttaaaatctt caacggaaac atattcactg aactgataat
     8341 ttcttttaaa atctttattg tatatcataa aaagtttgca tgtctccttt ccgaagatag
     8401 atattccata atctcgttat actttttata tctgcaagga ggacaccggg ataaatcaat
     8461 cctgtctatt acttcctgcc tctggtcact ttcccatata tccttaaaag atttttcttt
     8521 taaattacct attgaaaatt taggattgcc gcgtaactgg cagcaaagat aaacattgct
     8581 gtccgcacct atgatggaca ggatattatg agcaagacat ttctccggtc ttaaatcctc
     8641 ttcgtagaat tcccggaatc tgtgtttaaa tgtaatcagg tgcaatcctg tcgtatgtgc
     8701 cattttctga gcttcatcaa tctgtatatt aatcttttgc tttaccctgt ctgtgaaaac
     8761 aaggccgggc atataaacag gtcttacctg caggtaatcc acttttaaga tagaaagtat
     8821 tttgactaac tccgtaagtt tatagaaatt gatagtatgg agtaaaaagg caactcccag
     8881 aataagatct cctttttctt ttcctaatgc ctcaataccc tgaagaactc ttttaaatga
     8941 gttccatctg cccctatgca aggctttatg gtctgctgaa gatcctgcat caagggaaac
     9001 tctgcaaaac tggcagcaat cttttatagc ttgtatattt tcaattaata gctcaccgtt
     9061 tgttactact ccgcaatcaa aaccaagttc ctttgtttta tataaaacct ctttaaaatg
     9121 aggataaagt aatggttccc ctcctccgac ataggttata gcttttacgc ctatagcttt
     9181 taaatcctgt aaaagattaa agactaaatc tttctccaga gaaacaggat tttttgccct
     9241 gtaacggtca aacatgcacc atatgcagtt atggttacag agatttgtta aatcaatctc
     9301 aaccgccata ggaggagttt tattatcttt tatcatttca tttaaaacag gaacatgatt
     9361 taatatttta taagggttaa gaatatattt catttatttt aaaatcctct ccgtatacgg
     9421 ctttaagatt tttatttaca tcatgagtta aggtgtaatt aaaccactca aaaactttat
     9481 ctccccattt ccagtgaaga tattctccgt ctttcttgca gagttcttct attacaggat
     9541 ttttctttaa ctcattgcag gtaacaaaag tcttatgttg cgcgtaagac tgagggcaaa
     9601 accatatttc ataacctgcc agtttcattc taaattgtgt atcgaaatca caggaataaa
     9661 taaaaaagtt ttcatcaaat aatccaatat cttcgaacac ttcagaccgg aataaagtgc
     9721 aacccatatg cccccattct gcctgaaacg gtttaacatt gttaaataat tctacaggca
     9781 ttattcccat tctataatgc cagggattac tcatatccgg gccataattc tgaacgaagt
     9841 ttatgtttgt atagtccctg agagtattcg ataccatacc gcatttagga ttattttcca
     9901 taatgtcaat catgggcttt aagaaattct tactgacttt catatccgaa ttaatcaaac
     9961 atacataatt tgtatgggaa ttgcatatat cttttaatgc aatattccat ccttttgtcg
    10021 tgcctacatt tttctttaat cttcttacgg tacaactcac acagttattg aataaactgc
    10081 agaactcttc aaggaactca taacttccat ctgttgaacc ctggtcaata aagcgaatat
    10141 ctaccggata ttcattattt ttcaatgcct cttttaatga atggcataac tcagggataa
    10201 cactcatatt attacagttt aggataatta aataaactgt tctcatgctg ccacacgcct
    10261 ttctgtattt attctttctc ttactttctt tgcgacttca tcaggtttta ttgccgtcaa
    10321 acatggagga tatataccct gccagtttgt ttttaccgtt ctttgtttta atgataactt
    10381 attaaaaaca tattcacatt gatctccatg tgaaaaacaa aatttaccgc atagtccagg
    10441 attcttacag attacttcac aatttttata ataccttacc cttaaatcag ggttaaatga
    10501 tccgaataaa gcaattatat ttttctgaaa ggctcctgcc acatggagaa ctcccgtgtc
    10561 taccgttaca cataagtcca tgttgtaaac tatggagaac atctgccgga gtgaccatcc
    10621 tttgggtctt tgcggtgtcc ggccctcatc tctatgaata tataccacat tgaaatcatc
    10681 ttttaataaa tctcttaact tcttgaaata cggtataggc catgatctgt tctttgcaaa
    10741 actggcatca ggggaaactg atacaaaggg cttattcgga ttataaccca tagcaattaa
    10801 atccttatat ccccatgctt tttcttcctc tgtgagattt ataataagat ctttcggttt
    10861 tccttccggg tcaatatcaa cccatttata ataagcgtca attacatggt ctatctctgc
    10921 ctgcttatta tgttctattg ctccctgaaa tacacaggtt ttatcgtgag atgaaaatat
    10981 ctcaggtgta atagaaagtg gtaatacccc gtttacagga ggtctgtttt cctgttttaa
    11041 tccattttca aggcagtata ttttatctat atatggatta tttaccagag cttcccaata
    11101 cccgaaatgt aaaaagaagt ttatgttatg gcctaaagaa tgaagatatt taattatagg
    11161 agtgcaaaat aatatatctc caagtcctcc gagcctctga accagtatat ttaatttgcc
    11221 gtctcctaca atcttctttg gagtaatctc tttaaaagag catttatctc ccatctgttt
    11281 tatagagttc ctgagatatt caaccttttt aatatcctgg tctttaagtg aaagaaaatg
    11341 gttataaaat tgaaccattt ctgtttcact gcagggagtc actaaatttg gatttttctg
    11401 gcaaaaaaag gtatacatta ttcctctctc tccgggataa gaggggagga ttatcccctc
    11461 cccttcatcc ttattcgttc aagcccacaa tttgaccgga acttacggga gtgccgtatt
    11521 cgaaagtcat ttcgcaggta atcattctct ctactgtatt gtcaattttt gcgagaggtt
    11581 ccgcagttat cgggataagt tctgccctct tcatatcggt atgatcaacc atacagctta
    11641 cggctgcatc ttctgtggta ggaagatcat attctatgtc gatgttgcaa gtaaagtatc
    11701 cgttattgta actggacaca tct
//
LOCUS       DAOQWT010000004        10941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_6433_length_10941_cov_13.122267, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000004 DAOQWT010000000
VERSION     DAOQWT010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6433_length_10941_cov_13.122267"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            868..1173
                     /locus_tag="PKJ95_00255"
     CDS             868..1173
                     /locus_tag="PKJ95_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64705.1"
                     /translation="MNIKPKQFTRVISLRLNKQEYETVLSKFQRRKEDRISANIKRYL
                     FTKLSEEPVIEYSIRQKGDKMEVTIYDNGTAGDPLMYDNISLAMAYIRSINAIKRKE"
     gene            1170..1625
                     /locus_tag="PKJ95_00260"
     CDS             1170..1625
                     /locus_tag="PKJ95_00260"
                     /inference="COORDINATES: protein motif:HMM:NF039645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6075 family protein"
                     /protein_id="HNR64706.1"
                     /translation="MILLDIGFLNEDHKARFIDYCNRAHIHSGDVERKALFYILAGCN
                     DLVTKGINRMYDFQENIVKFSPVPEQMEKDFQPFSFCSSSHALLKLALNLYNSGYESL
                     SIADTFYNLDNNHRILAINAMLIRFNICLTAIEADPQQAIEQKIKNCLN"
     gene            1822..2001
                     /locus_tag="PKJ95_00265"
     CDS             1822..2001
                     /locus_tag="PKJ95_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64707.1"
                     /translation="MAKQVSMKQSYNYESITFRLLRTEKQSLKKLCEETGKSFSEICR
                     QGLELLSKNLKAGLI"
     gene            2136..2291
                     /locus_tag="PKJ95_00270"
     CDS             2136..2291
                     /locus_tag="PKJ95_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64708.1"
                     /translation="MFLDEIEETEEEEPEEEADPKPSLWERNRKHIKNFLDYWKKHRK
                     PMQLLLF"
     gene            2425..2850
                     /locus_tag="PKJ95_00275"
     CDS             2425..2850
                     /locus_tag="PKJ95_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HNR64709.1"
                     /translation="MIILVDNREQLPYFFKGYPAEVKRATLKTGDYSLEGYENKITVE
                     RKSKADLYMSLGRERDRFEAEFCRLREFDYAALVIESSLSGLLIPPARSQMNPKSIIQ
                     SLISWSIRYEVYVFFSDNRHYSECLIYSLFEKYLYNLEN"
     gene            3064..6075
                     /locus_tag="PKJ95_00280"
     CDS             3064..6075
                     /locus_tag="PKJ95_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64710.1"
                     /translation="MNSIEEFFNLLFKPGEIVEFRPIKPKWKGGGAGQSEFIKFNGDI
                     AGKIKDYIHRKNEDYNLYFGVIPRKGSGERSKVSRVNTCFVDIDSKDFTSEEEFTRHI
                     QTLENEILPSIDLKYTAKINSGHGFHYYFVLSKDEKLPIENNTCDAWRDVQCALIDIC
                     KADQAVGKDLPRILRLPGSMNIKEEGAFKDCKILEVHKENIYRYSHFLPVLKKHRKEV
                     LKKEEAKKPSVRVNYPALSMNEGEIIEKLQRVKNADKFNALFYKGDTSPYGNDTSRAD
                     QALVNMIAFYTKDEAVIDNIFRQSALYREKWDKETYKKSTIKKAIEGQTGTYDPNYGK
                     ALFNPSSVDNTTLAGIEVKPAIKSKPLPPSPAGEKEKPVKEKEDKPKKENVALNIFNA
                     IKDREELQTAWDGKKLYILTKNKKWSEVTGIGEMELLSKIYDITGMVALNKDTIKTIK
                     DLLKLKHYKEGKEKTDFFKRTAMIQDSIIYNGGDFTVKIRPGSWEIGEHEALFFENRN
                     QVQQEKPDVNYKEKNLMDLLKDIVPTKTDDELLLMAIYITSLFIPDIPKPALNIEGDH
                     GSGKTCIARVIKEIYDPGEVKSVNKINTEDLCLNFAVNDFMVLNNLSKINTSLSDILC
                     CIIDGETISKRKLYEDDSLFVKKVKGTFAITSISVGKKNADLLDRMLKINLQRIEDED
                     RKTEKKIGRHFRGKKPFILSSIFNILSEAMNYIEDIEEKEISRFRLADWYMWSLSIAR
                     ALDAEEKFKSIIEQQKKMMLEDQLQDDEKIEILYNYLMEQVTDRPFEISSKELKTQLN
                     EQSKNSKPLFTGHIGYFLKRVIVTFKGYGFNIENIRKAGGKRIWRVTKISDRVTNSDK
                     PENTSLSLKENHIPQGKKSISDRVTDDFFSLEKKEIEKRNLPLIEDNRNPISSEKSLS
                     LCHSCKKVSDSNNIISDKLSDKLISSSVTQKSLSLIKCSNCDKFTAKNRTAFFTCEIT
                     GKSIYPMAEHTCNNFNPVELAEDFDREEIPF"
     gene            6095..6475
                     /locus_tag="PKJ95_00285"
     CDS             6095..6475
                     /locus_tag="PKJ95_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64711.1"
                     /translation="MAKRGRPAFESDEDNFAAYKAVKTVYPELTTKRSFQNKCFELRA
                     IQAIAEEKRDIFKVLWNDKPDDRLTYKPPSSLLTELGRFENKELIIAIAEQLCNEMGE
                     LPNKPIAEWRRDLRQYRLNLIKGI"
     gene            6626..6784
                     /locus_tag="PKJ95_00290"
     CDS             6626..6784
                     /locus_tag="PKJ95_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64712.1"
                     /translation="MEKKKKKSKKELTVNVEKQEVISDKLHCPSCGVELPGDYVKRQE
                     EIQFEWPS"
     gene            7009..7398
                     /locus_tag="PKJ95_00295"
     CDS             7009..7398
                     /locus_tag="PKJ95_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64713.1"
                     /translation="MRDFAELKEVQRTLYNAKVGTKLFEEQNKVKAVKLFQKENNLPS
                     FEEALNKYDRAQKKVKEYSESNFSDFDQDIKDWIKQDRGQGFQCDIEDVKKVRELRTA
                     NKVQSFCEKNNCSQKQALHKLIMAGEI"
     gene            7399..8289
                     /locus_tag="PKJ95_00300"
     CDS             7399..8289
                     /locus_tag="PKJ95_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64714.1"
                     /translation="MKVTTEDLRELLQEQALTKEEVLEKLSISERTFYRLISEISDIS
                     KTDDRAPKYFINNKEMLIMAGVNNIIEEITLNLAEVGDSDATKGFSTGKGIELTIKDI
                     RIEKDNITLKLEDPKKNIMLQKLDYFENSGLLKSLLFSLSVDTKEPVKLNKKLFLNKK
                     VTGDVSFSECFNLKDYVEKEISRFASKEFKYFVTGFSPLTTQIFHLKLSEAVEAELQK
                     NIKILQGYNFLKKQELGALLQANNLPEETKNIFIKNSDKALSLSQIYSESEVVNKAIS
                     FLKDTIPEEARGNLVETILK"
     gene            9540..9845
                     /locus_tag="PKJ95_00305"
     CDS             9540..9845
                     /locus_tag="PKJ95_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64715.1"
                     /translation="MNIKPKQFTRVISLRLNKQEYETVLSKFQRRKEDRISANIKRYL
                     FTKLSEEPVIEYSIRQKGDKMEVTIYDNGTAGDPLMYDNISLAMAYIRSINAIKRKE"
     gene            9842..10297
                     /locus_tag="PKJ95_00310"
     CDS             9842..10297
                     /locus_tag="PKJ95_00310"
                     /inference="COORDINATES: protein motif:HMM:NF039645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6075 family protein"
                     /protein_id="HNR64716.1"
                     /translation="MILLDIGFLNEDHKARFIDYCNRAHIHSGDVERKALFYILAGCN
                     DLVTKGINRMYDFQENIVKFSPVPEQMEKDFQPFSFCSSSHALLKLALNLYNSGYESL
                     SIADTFYNLDNNHRILAINAMLIRFNICLTAIEADPQQAIEQKIKNCLN"
     gene            10494..10673
                     /locus_tag="PKJ95_00315"
     CDS             10494..10673
                     /locus_tag="PKJ95_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64717.1"
                     /translation="MAKQVSMKQSYNYESITFRLLRTEKQSLKKLCEETGKSFSEICR
                     QGLELLSKNLKAGLI"
BASE COUNT         4120 a         1583 c         2162 g         3076 t
ORIGIN      
        1 ggcaaaaaag aggcgttcag gccataggaa taataataac aattctcacg aatgggagaa
       61 ggagcaggat gaacttcaca gggaggagct ggataaggta aagactcagg cagaagagag
      121 ggagaaggct gttattgatg aatgtaagag gcaattataa gagtatatca ataaaccctg
      181 gtataggaag ttattctctt gaaggtgatc cagatgggag agatggcaga ctttaccctt
      241 aatagaaaca ggcttgatta cttgatttta cgcaaatgct tatcctgggc gagctgtgca
      301 gcggatagac aaaatcaatc aggataacac tctttgaatt atcttcaatt tcaaaatatg
      361 tgcagttgct aaacgtctga tgtgaactca ctacaatata gctatgtgct cataatctca
      421 ttcaggacac attcttaata tctcagtggt atatattcac cttcgatatg tttaaaacgc
      481 atttcttgaa acagtataat tgttgacttg acctgatttg aaactcaatt cgttgaacct
      541 tttttaagga attttccata ttcaataaac ttttattact aacaatgtcg ttaaatactt
      601 atttaatgac attatggatt ttaggaatgg cgttatatat agctttctaa aaatatattt
      661 tattgcgaca ttttacaaag aatggaaatc aggaaacagg cattatatat agtgttttga
      721 aaaatacctg actaaagtta tagggaatta aaataattgt ttttatatgt ctaaggaagt
      781 aaactttcat aaatatatta aaaaaaaaac tcaaaacagt ttactttcat aaatacctgt
      841 gttatcatct taaaaagggg tgataaaatg aatataaaac caaagcaatt tacaagagta
      901 ataagtttaa ggctgaataa acaggagtat gaaacagttt taagcaaatt ccagaggaga
      961 aaggaggata gaatttcagc aaatataaaa agataccttt ttactaaact ctcagaggaa
     1021 cctgtaattg aatattccat ccggcagaaa ggggataaaa tggaagttac catatatgat
     1081 aatggaacag caggagaccc gttaatgtat gataatatct ctcttgctat ggcttatatc
     1141 aggagtataa acgctattaa gagaaaggaa tgatattatt ggatatcgga tttttaaacg
     1201 aagatcataa agccaggttc attgattact gtaatagggc acatatccac agtggagatg
     1261 tggaaaggaa agccttgttt tatatactgg ctggatgtaa tgacctggta accaagggta
     1321 taaacaggat gtatgatttt caggagaata ttgttaagtt ctcacctgta ccggaacaga
     1381 tggaaaagga ctttcaacct ttctcttttt gttcatccag tcacgcatta ttaaaattgg
     1441 cattgaacct gtataacagt ggttatgaga gtttatccat agctgatacc ttttataact
     1501 tagataacaa tcacagaata cttgccatta acgccatgct gataagattt aatatttgcc
     1561 tgactgccat agaagcagat ccacaacaag ccatagagca gaagataaaa aactgtctga
     1621 attgattccc ctggagagag aagggaggag agtgaagtaa taaaacctca ctttcctttt
     1681 tttaatgctt agtgagactg aattaaattc atttagaatg aagcgaaatc attttggcga
     1741 aatcatttcg cttgttttct tacagaaaag attgctataa tacaatcgtt aaaagtaaga
     1801 ataagattag aggtgaaaca gatggcaaaa caagtcagca tgaaacagtc ctataactat
     1861 gagagtataa ccttcaggct tttgaggact gagaaacaat cgttaaaaaa actttgtgag
     1921 gagaccggga aaagtttttc tgaaatatgc aggcaggggc tggaacttct ctcgaaaaat
     1981 ttaaaagcag gattaattta acggattaac cctgctctat tactgccatt attggcaaaa
     2041 tttcaaacaa aatttgtttt atctatttgt atattatcag gtaaaacaag aaaagtaaac
     2101 tatttttgat aaaacttata ggaggtttta ccagtatgtt tcttgatgaa atagaagaga
     2161 cagaggaaga agaaccggaa gaagaagcag atccgaaacc ttcactgtgg gagaggaaca
     2221 ggaagcatat aaaaaacttt cttgattact ggaaaaagca caggaaacct atgcaactat
     2281 tactttttta gaggtaaatt atggttaaaa gaaaaaacct gcaagacagc aggagtagga
     2341 acaaaccaca taactataat agcaaaaaag cattgaattt gcaagggttt tcgctatttt
     2401 acaccttctt tttaggagaa ttttatgatt attttagttg ataacaggga gcagttgcca
     2461 tattttttta agggctatcc ggcagaagtg aagagagcca cattgaaaac cggggattat
     2521 tccttagaag gatatgagaa taaaatcact gtagaaagaa aaagtaaagc tgatttatat
     2581 atgtcacttg gccgggagcg tgacagattt gaggctgaat tttgccgtct gagagaattt
     2641 gattatgcag ctctggttat agagagcagt ctttcaggct tactcattcc acctgccagg
     2701 agccagatga atccaaaaag cataattcag agccttatat catggtcaat tcggtatgag
     2761 gtatatgtgt ttttttcaga caatcggcat tactcagaat gtttgattta tagtttattt
     2821 gagaaatatt tatataactt ggagaattga ggtaggattt tatgaggaag ctattgaata
     2881 gtcattgtca ggaaaatgaa tttctggata aaaacagaaa attctgtcag aggacagaac
     2941 ttttcagaac aatgttatta ttcaagaact tcctcatatt ttcctttatt ctgtctacat
     3001 aattttaaaa aatattttag gaggtaatta tgatgattaa tataaaagga gggcttattt
     3061 attatgaact ctatagaaga gttttttaat ctactgttta aacccggtga gattgtggag
     3121 ttcaggccca taaagccaaa atggaaaggt ggaggagctg gacaatcaga gtttataaaa
     3181 ttcaatggtg atatagccgg gaagataaaa gattatatcc acaggaaaaa tgaagattat
     3241 aatttatatt tcggagtaat tcccaggaaa ggatcaggag aaagaagtaa agtttcaaga
     3301 gtaaacacct gttttgtgga tatagatagt aaggatttta cctcagaaga agagtttacc
     3361 cggcatattc agaccttaga gaatgaaatt ttaccatcta ttgatttgaa atatactgcc
     3421 aagataaact caggtcatgg atttcattat tacttcgttc tcagtaagga tgaaaaatta
     3481 cctattgaaa ataacacctg tgatgcctgg agagatgttc aatgtgccct gatagatata
     3541 tgtaaggcag atcaagcagt agggaaagac cttcccagaa tattaagact gccgggaagc
     3601 atgaatataa aagaagaggg agcctttaaa gactgtaaga tccttgaagt tcacaaagag
     3661 aatatatacc ggtattctca tttcctgcca gtattaaaaa agcatagaaa agaagttcta
     3721 aaaaaagaag aagcaaaaaa gccatctgta agagttaatt atcctgcatt atccatgaat
     3781 gaaggggaga taatagagaa acttcagagg gttaaaaatg cagataaatt taatgcttta
     3841 ttttacaaag gagatacttc tccttatggt aatgacactt ccagagcaga tcaagcgctt
     3901 gtgaatatga ttgcttttta cactaaggat gaagcagtaa ttgataatat atttagacag
     3961 tcagcacttt accgggaaaa gtgggataaa gaaacttaca aaaaaagcac tataaaaaag
     4021 gccatagaag gacagacagg aacctatgat cctaattacg gtaaagcctt attcaatccc
     4081 tcctctgtgg ataataccac attagcaggt atagaggtaa agccggctat aaaaagtaaa
     4141 ccattaccac cgtcaccggc aggagagaaa gagaaaccgg ttaaagaaaa agaggataag
     4201 ccgaaaaaag aaaatgttgc cttaaatata tttaatgcca taaaagacag agaagaattg
     4261 cagactgcat gggatggcaa aaagctatat atcctgacta agaataaaaa atggtcagag
     4321 gttaccggca taggtgagat ggaattgcta tctaaaattt atgatattac cggcatggtt
     4381 gcattaaata aagacacaat caagacaata aaagaccttt taaagctgaa gcattacaag
     4441 gaaggtaagg aaaagactga tttttttaaa cgaactgcca tgatacagga cagcattata
     4501 tataacggtg gagattttac agttaaaatt agacctggtt catgggagat aggagaacat
     4561 gaagcattat tttttgagaa tagaaaccag gttcagcagg aaaagccgga tgtaaattac
     4621 aaggaaaaaa acctgatgga cttattgaaa gacattgtac ccacaaagac ggatgatgaa
     4681 ttactcctga tggctatata cattaccagt ttatttatac cggacattcc gaaacctgcc
     4741 cttaatatcg agggcgatca tggttcaggt aagacctgta tagcaagagt aataaaagaa
     4801 atctatgacc cgggagaagt aaaatctgtt aataagatta atactgaaga tctatgtttg
     4861 aactttgcag taaatgattt tatggtatta aataacctct cgaagataaa tacttcttta
     4921 tctgatatat tatgttgcat tattgacggt gagaccatct caaagagaaa actttatgaa
     4981 gatgatagtt tatttgtgaa gaaggttaag ggcacatttg ccattaccag tatatcagtc
     5041 ggaaagaaaa atgcagatct tcttgataga atgttaaaga taaaccttca aagaattgaa
     5101 gatgaggaca gaaaaacaga gaagaaaata ggaaggcact tcagaggcaa gaaaccattt
     5161 attttatcat caatatttaa tatcctgtca gaggctatga attatataga ggatatcgag
     5221 gaaaaagaaa tatcacgttt caggctggct gactggtata tgtggagttt atcaatagca
     5281 agggccctgg atgcagaaga gaagtttaaa agcattatag aacagcagaa aaagatgatg
     5341 ttagaagatc aacttcagga tgatgagaaa attgaaattc tttataatta cctcatggag
     5401 caggttacag acagaccttt tgaaatttcc tcaaaagaat taaagaccca gttaaacgaa
     5461 cagtcaaaaa attcaaaacc cttattcact ggacatatcg gttatttctt aaagagggtg
     5521 atagtgacct ttaagggcta tggttttaat attgaaaata ttcgtaaggc cgggggtaaa
     5581 agaatttgga gagtgacaaa gattagtgac agagtgacaa acagtgacaa accagaaaat
     5641 acaagtttgt cactaaaaga aaatcatata ccacaaggaa aaaaaagtat tagtgacaga
     5701 gtgacagatg attttttctc cttagagaaa aaagaaatag aaaaaagaaa tcttcctctt
     5761 attgaagata atagaaatcc tataagtagt gaaaaaagtt tgtcactctg tcactcatgt
     5821 aaaaaagtga gtgatagcaa taatattatt agtgacaaac ttagtgacaa acttatatct
     5881 tcatctgtca ctcaaaaaag tttgtcactg ataaaatgtt ctaactgtga taaatttact
     5941 gcaaaaaata ggactgcttt tttcacctgt gagattaccg ggaaaagtat ttatcctatg
     6001 gcagaacata cctgtaataa ttttaatcct gtggagctgg cagaagattt tgaccgggaa
     6061 gaaattccat tttaatgaaa gggaggtctt ttcagtggca aaaagaggaa ggccggcttt
     6121 tgagagtgac gaagataatt ttgcagcata taaagcagta aaaacggtat atcctgagtt
     6181 aactacaaag agaagttttc agaataaatg ttttgaatta agagctatac aggcaatagc
     6241 agaagagaaa agggatatat ttaaggtatt atggaacgat aaacctgatg acagattaac
     6301 ctataaacca ccttcatctt tgttaactga attaggcaga ttcgagaata aagaattaat
     6361 aatagcaata gcagaacagt tatgtaatga aatgggagaa ttgcctaata aacccatagc
     6421 agaatggagg agagacttaa gacaatatag attaaattta ataaagggaa tttaggaaac
     6481 cgtttcttaa attactttcc tggaaagggc agaatatttc ttaaaacttc aatcactagg
     6541 ttttactagt gcttaaggta gtaaaaagta gtaatatagt aagtgaacaa tttatcggta
     6601 taccataaaa aaaggagtga tattcatgga aaagaagaaa aagaaaagta agaaagaatt
     6661 aactgttaat gtggaaaaac aagaggtgat tagtgataaa cttcattgtc cttcctgtgg
     6721 agtagaatta ccgggggatt atgtgaaaag acaagaggaa atacaatttg aatggccatc
     6781 ctgacatcct gacacgatat atagttatta aagtgaatcg gctatactga ctttaaatac
     6841 cgggttaagg cagatatgat atcatgactt ttcttaccgg gttgaggcat attttctatt
     6901 aaaaaaactt ccacttaata cctaatattc ttcttttaac tcttactata actatggtaa
     6961 aaagccttgt tttatgctat aatagaagtg taaaggagag tgaattttat gagagatttt
     7021 gcagaattaa aagaagtcca gaggacacta tataacgcaa aggtaggaac taaactattt
     7081 gaggaacaga ataaagtaaa agcagtaaag ctgtttcaaa aagagaataa tctaccttcc
     7141 tttgaagaag ctttaaataa gtatgacagg gcacagaaga aagttaaaga atattcagaa
     7201 tctaactttt cagacttcga ccaggatata aaagactgga taaaacagga cagaggacag
     7261 ggctttcaat gtgatattga agatgtaaag aaagtaagag aattaagaac agcaaacaaa
     7321 gtgcaatcct tctgtgagaa aaataactgt agtcagaaac aggcactgca taaacttata
     7381 atggcaggtg agatataaat gaaggtaact acagaggact taagggaatt gctacaggag
     7441 caggcactga caaaagaaga agtcctggag aaactttcaa tcagtgagag aaccttctac
     7501 aggcttatat ctgaaatatc agacatatca aaaacagatg acagagcacc gaaatatttt
     7561 ataaacaata aggagatgtt aataatggca ggggtaaata atataataga agagataact
     7621 ttaaatctgg cagaggtagg agatagtgac gcaacaaaag gattttccac cggcaagggc
     7681 attgaattaa cgataaagga tataaggata gagaaagata atatcacatt gaagctggaa
     7741 gatccaaaaa agaatatcat gttgcagaag ctggattact ttgaaaattc aggactgctg
     7801 aagagtttac ttttttctct atctgtggac acaaaggaac ccgttaaact taataaaaag
     7861 ctattcctga ataaaaaggt aacaggtgac gtctcctttt cagagtgttt taatctcaaa
     7921 gattatgttg aaaaagaaat ttcccgattc gcctcaaaag aatttaagta ttttgtaacc
     7981 ggcttttcac ctttaactac tcaaatcttt catctgaagt tatcggaagc tgtggaagca
     8041 gaattacaaa aaaacattaa aatccttcaa ggttacaatt tcctgaagaa acaggaatta
     8101 ggggcacttc tccaggcaaa caatctaccg gaagaaacaa agaatatctt tattaaaaat
     8161 tcagataaag cactttcact ttctcaaata tactctgaaa gtgaagttgt gaataaagca
     8221 atttctttct taaaagacac aatccctgaa gaagcaaggg gcaacctggt agaaactatt
     8281 ctaaaataaa cttttggcct ccaaaatcaa aggtttttgc ttaggcaggt ctcttcacct
     8341 gccgattttt tttgttggaa aagtgattac ttttgaagta accgcttcct taaatcaggt
     8401 tgtctgaaat cagataaccc ggttgatggg ataaatcaat atacaaaagt ggaactagct
     8461 caaaatgatc cttttgattt cacaaatcaa tacgtgaaaa gtgaaaaagc tcaaaatgat
     8521 ccacacatgt gattaatctg cgattaacct gaaaacctga taacacctaa acaccggaaa
     8581 aacccctgcc atgtgcaaaa ttaacgggct tgttcacaca aaaaatatac ttccaaaaca
     8641 ccgcactcag agccgtttta gaccggtttt aaggcaaaaa agaggcgttc aggccatagg
     8701 aataataata acaattctca cgaatgggag aaggagcagg atgaacttca cagggaggag
     8761 ctggataagg taaagactca ggcagaagag agggagaagg ctgttattga tgaatgtaag
     8821 aggcaattat aagagtatat caataaaccc tggtatagga agttattctc ttgaaggtga
     8881 tccagatggg agagatggca gactttaccc ttaatagaaa caggcttgat tacttgattt
     8941 tacgcaaatg cttatcctgg gcgagctgtg cagcggatag acaaaatcaa tcaggataac
     9001 actctttgaa ttatcttcaa tttcaaaata tgtgcagttg ctaaacgtct gatgtgaact
     9061 cactacaata tagctatgtg ctcataatct cattcaggac acattcttaa tatctcagtg
     9121 gtatatattc accttcgata tgtttaaaac gcatttcttg aaacagtata attgttgact
     9181 tgacctgatt tgaaactcaa ttcgttgaac cttttttaag gaattttcca tattcaataa
     9241 acttttatta ctaacaatgt cgttaaatac ttatttaatg acattatgga ttttaggaat
     9301 ggcgttatat atagctttct aaaaatatat tttattgcga cattttacaa agaatggaaa
     9361 tcaggaaaca ggcattatat atagtgtttt gaaaaatacc tgactaaagt tatagggaat
     9421 taaaataatt gtttttatat gtctaaggaa gtaaactttc ataaatatat taaaaaaaaa
     9481 actcaaaaca gtttactttc ataaatacct gtgttatcat cttaaaaagg ggtgataaaa
     9541 tgaatataaa accaaagcaa tttacaagag taataagttt aaggctgaat aaacaggagt
     9601 atgaaacagt tttaagcaaa ttccagagga gaaaggagga tagaatttca gcaaatataa
     9661 aaagatacct ttttactaaa ctctcagagg aacctgtaat tgaatattcc atccggcaga
     9721 aaggggataa aatggaagtt accatatatg ataatggaac agcaggagac ccgttaatgt
     9781 atgataatat ctctcttgct atggcttata tcaggagtat aaacgctatt aagagaaagg
     9841 aatgatatta ttggatatcg gatttttaaa cgaagatcat aaagccaggt tcattgatta
     9901 ctgtaatagg gcacatatcc acagtggaga tgtggaaagg aaagccttgt tttatatact
     9961 ggctggatgt aatgacctgg taaccaaggg tataaacagg atgtatgatt ttcaggagaa
    10021 tattgttaag ttctcacctg taccggaaca gatggaaaag gactttcaac ctttctcttt
    10081 ttgttcatcc agtcacgcat tattaaaatt ggcattgaac ctgtataaca gtggttatga
    10141 gagtttatcc atagctgata ccttttataa cttagataac aatcacagaa tacttgccat
    10201 taacgccatg ctgataagat ttaatatttg cctgactgcc atagaagcag atccacaaca
    10261 agccatagag cagaagataa aaaactgtct gaattgattc ccctggagag agaagggagg
    10321 agagtgaagt aataaaacct cactttcctt tttttaatgc ttagtgagac tgaattaaat
    10381 tcatttagaa tgaagcgaaa tcattttggc gaaatcattt cgcttgtttt cttacagaaa
    10441 agattgctat aatacaatcg ttaaaagtaa gaataagatt agaggtgaaa cagatggcaa
    10501 aacaagtcag catgaaacag tcctataact atgagagtat aaccttcagg cttttgagga
    10561 ctgagaaaca atcgttaaaa aaactttgtg aggagaccgg gaaaagtttt tctgaaatat
    10621 gcaggcaggg gctggaactt ctctcgaaaa atttaaaagc aggattaatt taacggatta
    10681 accctgctct attactgcca ttattggcaa aatttcaaac aaaatttgtt ttatctattt
    10741 gtatattatc aggtaaaaca agaaaagtaa actatttttg ataaaactta taggaggttt
    10801 taccagtatg tttcttgatg aaatagaaga gacagaggaa gaagaaccgg aagaagaagc
    10861 agatccgaaa ccttcactgt gggagaggaa caggaagcat ataaaaaact ttcttgatta
    10921 ctggaaaaag cacaggaaac c
//
LOCUS       DAOQWT010000005         9979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_7251_length_9979_cov_3.478134, whole genome shotgun sequence.
ACCESSION   DAOQWT010000005 DAOQWT010000000
VERSION     DAOQWT010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9979
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7251_length_9979_cov_3.478134"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PKJ95_00320"
     CDS             <1..457
                     /locus_tag="PKJ95_00320"
                     /inference="COORDINATES: protein motif:HMM:NF025099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HNR64718.1"
                     /translation="FLITSLDTATRLGRYCFEELTAEAMPSISNRYMGTIITVVAGGG
                     LAMSGQWSAIWPIFGSANQLLAGLALLSATAWLAHLGKNFKITYYPMAFMMLVTVAAL
                     INLVFTNFGKKNYLLGSISILLLILASFVVYNGFKALGKLQEKIAAVKD"
     gene            663..1289
                     /locus_tag="PKJ95_00325"
     CDS             663..1289
                     /locus_tag="PKJ95_00325"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNR64719.1"
                     /translation="MYTFQSKYIAVEGAIGAGKTSLVLKLSKRYHARSILEVVEENPF
                     LSHFYDDIRDYAFRTQIFFLLSRYDQQVKATQQDLFKQLSFADYMFAKDRIFAHLTLS
                     GNELTMYEHLYQIMCRDIPPPDLIVYLRASTRLLMKRIMLRDRPFERKITVQYMEKLN
                     NCYEDYFSNINNFLHTRLVIINADELDFVGNSRDLELVCQKIVQKDQE"
     gene            1327..1980
                     /locus_tag="PKJ95_00330"
     CDS             1327..1980
                     /locus_tag="PKJ95_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNR64720.1"
                     /translation="MKKFIIVSGNIGSGKSSLTKILSEQLGWKAFYEVVENNPYLEDF
                     YNDMKKWSFHLQVFFLSKRFQHHQHIVQDPHSVIQDRSIYEDVDIFAKNLFEQGLMEP
                     RDYNNYCELFNIMVDFLSPPNLIIYLQASVDTLKRRIALRGRDYERSMSRGYLEQLNI
                     LYERWVKKFTLCPILTVPTDDLDFVQNSKHLQLITKNILTKLEGVEKTSCDRTDIWD"
     gene            1977..2981
                     /locus_tag="PKJ95_00335"
     CDS             1977..2981
                     /locus_tag="PKJ95_00335"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNR64721.1"
                     /translation="MNSKLMEINHKNLDFPILYYLSEEHSINKTELSNKLLLTKTDIE
                     KQVEQLSQLGYHLSIDAKKGYRIVKRPDILLPFEIKKKLTTSYIGQTIYYFPELSSTN
                     TMANQMAGEMSGKMPEGTIIIAEKQSGGKGRSGKKWISPPGGIWSSIIFYPLDISTHI
                     SLFTLMAAAAVARTIDKLFPQIRVHIKWPNDILTGGRKICGILTEMSTEKKNVKWVIV
                     GIGINVNNDSSCLPEEIRENSLSLKEITGHPINRINLISHLCSEIERFYEAFKNKDYS
                     LIIEEWKTYNNVIGKKIKVNLGEKIIIGEAIGIDEKGALILKTDTAEIIEIISGIILP
                     "
     gene            3020..7135
                     /locus_tag="PKJ95_00340"
     CDS             3020..7135
                     /locus_tag="PKJ95_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNR64722.1"
                     /translation="MSISQKNDKEIIMGKLLSDEQKWLLEISSNHFGINQRIKNLLAM
                     IHYSPHKSKMIHDGLREIALNDLWFYKTHQESQKAFQAILTLFNNLLNTVLDKQDKWL
                     ILNTLMEFILELFQNNGIEVGTLVEEIVVILENTLFNDLKIKIYASSLLKKLPVHIMK
                     ERDYIRRMEDLLRGSLNENISMWRKHLNFQHWSERHDELFSEKYYDDIVSVTKKEKQL
                     IEEIENKLSQADSWKELENITCFKEFLNELTSLIEKDTTVLENIYFLYYLLGIPAMFG
                     FKDTLLIELAHLYTRFTGEDLKELPLETFLNNTFSLFNSLKTENTEKILECLLKLGQK
                     IYETEEQSYIDIFVEYLIRFGFIYPGEIRINDEWQIQTDNNHLKMIRLWFHLIKCSPC
                     HSSKLIAALIVNLREGGVLINDNDLFQRDISQLLNSDIKPNYILIKQLLNLFPVYFDT
                     IGAEGKIREISTEIDKISFSEDKLIHFLRKQVHTESNNTQIELIRRIFNFWFDKNNEP
                     LLEYLPQDVQRELSLSDKWFTEVHNTVNELCNTLKLTPEGILEENIDEIKKLLIQINK
                     FEGSGGKRVVALIELYQLLKHKYTLDYKYIFLDLRKSHFFTGKEIEKLERNIKNNRYS
                     TAIKELFKMISGLKRIILESPRTDAQESIYLKRHIASGIPSMYGQYSEQKFDAIGLIL
                     RLERFTDYLINKFIAQRDLEYMTIDGFHSAARILDLFKEGLSLNGICSENFNSHLEML
                     NYSFKTTTFSMDQFVNIFSFITLNVKEIIDTYYILPFRAPLRTIIKQNIKDVNIVDET
                     IQEHQIYRKSEEFYRKMIVSAFLVQSLDNYVSKILSTLKNMLGRLKPEVIQMLLNYNP
                     KLLTTNLHKPNLRVDNQIFLGAKAYYLKKLYSYKFPIPPGFVLTTEWFRDREAIGQFP
                     EMYQAILGKIKDKLAELERMTKKRFGDKNNPLLLSVRSGTVISMPGAMDSILNIGMND
                     EIVESLSKIPGYGWAAWDSYRRLLQNWGMAHGITRDEFDKLILKYKGIYQVKEKREFS
                     NDQIKEIAFSYKKLLLDYGVKLEEEPFKQLIQAIIFVFQSWYNDRAQMFRKKLQIAEE
                     WGTAVIVQEMILGNMNAKSGTGVFFTKIPFEKSSEVTLYGDFTCCSQGEDIVSGLVYT
                     LPISEFQNEKFPHLKGESLEVQFPEIYRRLLSLAKELIYERGYEHQEIEFTFKSKRKK
                     DLYILQTRPYVLKDIEKIPTFTSPDILDYLVGTGIGIGRGAINGRIAFNLEDIRMLLK
                     KYPQDHKILIRPDTVPDDIEMIFECDGLLTARGGVTSHAAVTAAQLGKICVVNCKHLI
                     VLEKEKKCFINNNEFKTGDKVAIDANLGNIYKGHHVIDLEQISYFDERGNQ"
     gene            7116..8384
                     /locus_tag="PKJ95_00345"
     CDS             7116..8384
                     /locus_tag="PKJ95_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNR64723.1"
                     /translation="MKGEISKLDFKNRKKCLGINGLGRIGKLTLWYQLHENYFDSFII
                     NVGRPVGKSLQNIVDLITADSTYGAIEKFLFGIKAERDIKIIDEKENLLEIFGKPVKI
                     LCEARNPKNIGWREYGVKLVVDSTGVFNDPTVAAEGNEKGSLRGHLSAGAEKVINSAP
                     FKIKDKSKKIPEDSTTLIYGINHISYNQNKHNIISAASCTTTALAHMVKPLLEREETK
                     NILTASLSTIHAATNSQSVLDSLPGAGDTDLRKNRMVFNNIILSTTGAASTLEKVLPE
                     VKRVGFMADSVRIPTDTVSLIILNLTFHSTLDKKGDSTINREFINQIYHDASQGAQRE
                     LLCYSDNQNVSSDLKGKLSAVTIEGHATHTRTGFIGLNPQMLQDVGIVTGRIIDIPIT
                     HLTVFGWYDNEYGSYVHCLGKLATYIHRNL"
     gene            8421..8990
                     /locus_tag="PKJ95_00350"
     CDS             8421..8990
                     /locus_tag="PKJ95_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNR64724.1"
                     /translation="MKEFRIVSLPMKKINIHQMILVSLFAALTAVGAFISIPIGLVPI
                     SLQNLFTLLSGMVLGSRMGTFSQLIYILLGVAGLPVFSGFRAGLGVILGPTGGFLTGF
                     VISTYIVGKILEKIADARVWSYFVAGIVGIIIIYTCGIMQLLIVTQIGLKEAVLIGIV
                     PFLPGDFIKLITASFLAIRIKSVVSLPQV"
     gene            9013..9090
                     /locus_tag="PKJ95_00355"
     tRNA            9013..9090
                     /locus_tag="PKJ95_00355"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9048..9050,aa:Pro,seq:cgg)
     gene            9229..>9979
                     /locus_tag="PKJ95_00360"
     CDS             9229..>9979
                     /locus_tag="PKJ95_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNR64725.1"
                     /translation="MYQTKTLLTIKEASQWASNFLKREISESNISYLVQYGKVRKYNG
                     GNCALIDLNDLKYYYQSFRGWREISWKRKLGEDLNWNLSFDYLKEKDTTKHVHRLHPY
                     KGKFIPQLVQYFIDNHTDDFKKDVYFKPGDIILDPFLGSGTTLVQANEMGMHSIGVDI
                     SRFNCLIAETKLLDYDFISLEKEIKKIQKAIVEFETESNIEGFEGELLFKLAEFNKKY
                     FPSPEFKYKVNRKQIEEGKYGTEKEQEFLSIY"
BASE COUNT         3411 a         1518 c         1957 g         3093 t
ORIGIN      
        1 atttttaatt acctctttag atacagcaac ccgtctggga aggtactgtt ttgaagagtt
       61 aactgctgag gcgatgccct ctatttctaa ccgttatatg ggaactatca taactgttgt
      121 tgccggtgga ggattggcaa tgagtggaca atggtcagct atctggccca tatttggttc
      181 agcaaatcag ttactggccg gattagctct tttaagtgcc actgcctggt tggcacatct
      241 gggtaaaaac ttcaaaatta cctattatcc aatggctttt atgatgttag ttaccgtggc
      301 ggctttgata aatttggtat ttaccaattt tggtaaaaag aattacctgt taggctcaat
      361 ttctattcta cttttaattc tggccagttt tgtggtctat aatgggttca aggcactggg
      421 taaattacag gaaaaaatag cagctgttaa agattagtaa aaaattctaa aaataaaaag
      481 agagtaaatt cactctaagt taagaacaga ttaatgccct aactttataa tataaaacaa
      541 ggctggcata agtcagcctc ttttaccatt tttgctgttc tgtttttaat tattaattat
      601 atgcttacca ttataatacc gataatattc gacacccaat taaaaaaaga gatttaacct
      661 aaatgtatac atttcagagc aaatatattg cggttgaggg agctattggt gccgggaaaa
      721 caagtttagt gctgaaattg agtaaaagat atcatgcccg gtccatattg gaagtagtag
      781 aagaaaatcc ttttttatct catttctatg atgatataag agattatgct tttcgaactc
      841 agattttctt tttattaagc cgttatgacc agcaggtaaa agccactcaa caagaccttt
      901 tcaagcaatt atcatttgct gactatatgt ttgcgaagga caggatcttt gcccatctaa
      961 cgctttctgg gaatgagcta actatgtatg aacatttata tcaaataatg tgccgcgaca
     1021 tacctcctcc cgatttaatt gtctatctaa gagccagcac cagattgctt atgaaaagaa
     1081 taatgttgag agaccgtcct tttgaacgaa aaatcactgt tcaatatatg gaaaaactga
     1141 acaactgcta tgaggattat tttagtaata taaacaactt tcttcatacc agactggtta
     1201 tcattaatgc cgatgagttg gattttgtgg gaaatagccg ggatttagaa ttggtttgcc
     1261 agaagatagt gcagaaggat caagaataaa ataaaagaga aaagagattg aaaaacagtg
     1321 atgaaaatga aaaaattcat tattgtttct ggaaacattg gaagcggaaa gtccagtctg
     1381 actaaaattt tgagtgaaca gctgggatgg aaagcttttt atgaggtggt tgaaaataac
     1441 ccttatttag aagattttta taacgatatg aagaaatgga gttttcattt acaggttttt
     1501 ttcttatcca aacgtttcca acatcaccag catattgttc aagaccctca ttccgtgata
     1561 caggaccgta gtatttatga ggatgttgac atatttgcaa agaatttatt cgagcagggc
     1621 ttgatggagc cacgggatta taataattat tgtgagttat ttaatattat ggtagatttt
     1681 ttatctcctc caaacctgat aatttatttg caggcttctg ttgacacctt aaagcgaaga
     1741 atagctctaa ggggcaggga ttatgaaaga agtatgtcca gaggctattt ggagcagctg
     1801 aatattttgt atgagagatg ggtaaaaaaa tttacccttt gccccattct gactgtacca
     1861 accgatgatc ttgattttgt acagaattcc aaacacctgc aattgattac caaaaatatt
     1921 ttaaccaaat tagagggagt ggagaaaacc tcttgtgata gaacagatat ctgggattga
     1981 atagcaaatt gatggaaata aatcataaaa atctggattt ccccatacta tactatcttt
     2041 cagaagaaca ttctataaat aaaacagaat tgagcaacaa actgttgctt actaaaacgg
     2101 atattgaaaa acaggtagaa caattaagtc aacttggata ccatctctct attgatgcta
     2161 aaaaaggtta tcgcattgtt aagcgtcccg atattttatt gccttttgaa ataaagaaaa
     2221 aattaactac cagctatatt ggacaaacca tctactattt tcctgaacta agctcaacca
     2281 atactatggc taatcagatg gcaggagaaa tgagcgggaa aatgccggaa ggaacaatta
     2341 tcattgctga aaaacagagt ggagggaagg gcaggtctgg gaaaaaatgg atttctcctc
     2401 caggggggat ctggtcttct ataatatttt atcccttaga tatttctacc catatatctc
     2461 tctttaccct gatggctgct gccgctgtag cacgaactat tgataaattg tttccacaga
     2521 tcagagtaca tattaaatgg cctaatgata ttttaacagg tggtagaaaa atatgtggta
     2581 tactgaccga aatgagcaca gagaaaaaaa atgttaagtg ggtcatagtg gggataggta
     2641 taaatgtaaa taatgattct tcatgcctgc ctgaggaaat cagagaaaat tccctttcct
     2701 taaaggaaat taccggacat cccataaacc ggattaattt aatcagtcat ctttgcagtg
     2761 aaatagagag attttatgaa gcgtttaaaa ataaagatta ttctctgatt atagaagaat
     2821 ggaagaccta taataatgta attggaaaaa agataaaagt gaatttggga gaaaagataa
     2881 ttatcggtga agctattggt attgatgaaa agggtgcctt aatcttaaaa accgatactg
     2941 cggaaattat agaaataata tccggaatta ttttgcctta aaatagagga aagcagagga
     3001 ggatttcaaa caagaaaaga tgtcaatttc tcaaaaaaat gataaagaaa taataatggg
     3061 caagcttcta tctgatgaac aaaagtggct cttagaaata tcttctaacc attttggtat
     3121 aaatcaacga ataaaaaatc tcctggctat gattcattat tctccgcata agtcaaaaat
     3181 gattcatgat ggattgaggg agatcgcctt aaatgattta tggttttata agactcatca
     3241 ggaatcacaa aaggctttcc aggccatctt aaccttattc aataatctgt taaacactgt
     3301 gctagataag caggacaaat ggctaatttt gaatacttta atggagttta ttctggagct
     3361 gtttcaaaat aacggaattg aagttggcac tctcgttgaa gaaatagttg ttattttgga
     3421 gaatacttta ttcaacgatt taaaaattaa aatatatgcc tccagtttgc tgaagaaact
     3481 tcctgtgcat attatgaagg agagggatta tatacggaga atggaagatt tactgagggg
     3541 atctctcaat gaaaacatat ctatgtggcg aaaacacctg aatttccagc attggtctga
     3601 aaggcatgat gagctttttt ctgaaaaata ttatgatgat atagtatctg tcactaaaaa
     3661 agaaaaacag ctcatagaag agattgagaa taagttatct caagccgata gctggaagga
     3721 attagaaaat attacctgtt ttaaagaatt cctcaatgag ttgacctctt taatagaaaa
     3781 agataccacg gttttagaaa atatttattt tctttattac ttgctgggaa tcccggcaat
     3841 gttcggattt aaggatactt tattaataga gttagctcac ctgtatacca gatttacagg
     3901 tgaagacctc aaagagctac ctctggagac ctttttaaac aataccttct ctctatttaa
     3961 cagtttaaag actgaaaaca cagaaaagat tctggaatgt ttattgaaat taggtcaaaa
     4021 gatttatgaa acagaagagc agtcttatat agatattttt gtggaatacc ttatcagatt
     4081 tggttttatt tatcctggtg aaattagaat taacgatgag tggcaaattc agaccgataa
     4141 taatcatctt aagatgatca gattatggtt tcatttaatc aaatgttccc cctgccatag
     4201 cagtaagctg atagcagctc tgattgtaaa tttaagagaa ggtggagtac ttataaatga
     4261 taacgaccta ttccagagag atataagtca actgctcaat agtgatatta aaccaaatta
     4321 tatcttaata aaacagttat taaacctttt cccggtctat tttgacacca tcggagcaga
     4381 gggaaagata agggaaatat cgacagagat agataagata tcttttagtg aagataaatt
     4441 aatccatttt ttaaggaaac aggttcatac tgaaagcaat aatacccaga tagaactgat
     4501 tagaaggatt tttaatttct ggtttgacaa aaataatgaa ccccttttag aatatcttcc
     4561 acaggatgtc caaagagaat tatctttatc agataaatgg tttactgagg tgcataatac
     4621 cgtaaatgag ctctgtaata cactgaagtt aacccctgag ggcatattgg aggagaatat
     4681 tgatgaaatt aaaaaattgc taattcagat taataagttt gaaggtagtg gtggtaaaag
     4741 ggttgttgct ctcatcgaac tctatcagct cttgaaacat aaatataccc tcgattataa
     4801 atatatcttt cttgatttac gaaagtccca ttttttcacc ggtaaagaaa tagagaagtt
     4861 agaaaggaat ataaaaaata atagatatag cactgccatt aaagaacttt ttaaaatgat
     4921 ctctggctta aagaggatca ttctggaatc ccccaggact gatgcccagg aaagcatata
     4981 tcttaagcgt cacattgcct cagggatacc ctctatgtat ggacaatact ctgaacaaaa
     5041 gtttgatgcg atagggttga ttttacgttt ggaaagattt accgactacc ttatcaataa
     5101 atttattgct caacgggatc tggaatatat gaccatcgac ggtttccata gtgctgccag
     5161 gattctggat ttatttaaag agggattaag tttaaatggt atttgcagtg agaactttaa
     5221 ttcccatctg gaaatgctca attatagttt taaaaccaca actttctcta tggatcaatt
     5281 tgtaaatatt ttttctttta ttaccctgaa tgttaaagaa ataattgata cttattatat
     5341 attaccgttc agagcccctc tgagaacaat tatcaagcag aatattaaag acgttaatat
     5401 tgttgatgag acaattcagg aacatcagat ctatcgaaaa tcagaagaat tttatcgtaa
     5461 aatgatagta tcagcctttt tagtacagag cctggataac tatgtctcca aaatacttag
     5521 taccttaaaa aatatgctgg ggagattaaa accggaagta atacagatgc tgctcaacta
     5581 taaccccaaa ctgcttacta ccaacttgca taaacccaat ctcagagttg ataaccagat
     5641 ttttttaggt gccaaagcct attatctgaa aaaattatat tcttataaat tccctattcc
     5701 cccgggtttt gtacttacta cagaatggtt cagggacaga gaggcgattg gacaatttcc
     5761 tgaaatgtat caggcaatat tgggaaagat aaaggataaa ctagctgaat tggagagaat
     5821 gactaagaag agatttggag ataaaaataa tcctctctta ctctcagtcc gttcaggaac
     5881 agtaatttct atgcccggtg ctatggattc cattttaaat attggtatga atgatgagat
     5941 tgtggaaagt ttaagtaaaa ttccgggcta tgggtgggca gcctgggatt cttatcggag
     6001 attgttgcaa aattggggca tggcacatgg gataacaaga gatgagtttg ataaacttat
     6061 cttaaaatat aaaggaatat atcaagtaaa agaaaaaaga gaatttagca atgaccagat
     6121 taaagagatc gctttttctt ataaaaaatt gctgctggat tatggggtta aattagaaga
     6181 agagcctttt aaacagctga ttcaagcgat aatatttgta tttcaatcct ggtacaatga
     6241 tagagcccag atgtttcgta aaaaactgca gattgccgag gaatggggta cagctgtaat
     6301 agtgcaggaa atgattttag gcaatatgaa tgctaaatca ggcaccggtg tattctttac
     6361 caagatacca tttgagaaga gttctgaggt tactctctat ggtgatttta cctgttgcag
     6421 tcagggggaa gatatagtat cagggttggt ctacactctg ccaatttctg aattccaaaa
     6481 tgaaaaattt ccccatctta aaggtgaatc tctggaagtg caatttccag agatttatcg
     6541 gagattattg agtttagcta aagaattgat ttatgagaga gggtatgaac accaagagat
     6601 tgaatttact tttaaatcaa aacgtaaaaa ggatttatat attctacaaa ccagaccata
     6661 tgttttgaaa gatatagaaa agattcctac ttttaccagt ccggatattc tcgactatct
     6721 ggtgggaaca gggattggaa taggaagagg agcaataaat ggtcgtattg cctttaactt
     6781 agaagatatc cggatgctat taaagaaata ccctcaagac cacaagattt taattcgacc
     6841 ggatacggtg cctgatgata tagagatgat ttttgaatgc gatggactgt taactgcacg
     6901 cggcggagta acctctcatg ctgccgttac agctgctcag ctgggtaaaa tttgtgtggt
     6961 gaattgtaaa catttaatag tattggaaaa agaaaagaaa tgttttatta ataataatga
     7021 atttaaaaca ggggacaaag ttgccataga tgctaacctg gggaatattt ataaaggtca
     7081 tcatgtcatt gatttagaac agatatccta ttttgatgaa aggggaaatc agtaaattgg
     7141 actttaaaaa tagaaaaaaa tgtctgggta tcaacggact aggccgtatc ggcaagctaa
     7201 ctttatggta tcagcttcat gaaaattatt tcgatagctt catcataaat gtagggcgtc
     7261 cggtgggaaa gagtctgcaa aatattgtgg atttgataac tgctgattct acttatggtg
     7321 caatagaaaa gtttttattt ggaataaaag cagagagaga tattaaaatt attgatgaaa
     7381 aagagaatct tttagagata ttcgggaaac cggtgaaaat attatgtgag gcaagaaatc
     7441 ctaaaaatat tggctggaga gaatatggag taaaattagt cgttgattct accggggttt
     7501 ttaatgatcc cactgtcgct gctgaaggca atgaaaaagg tagcctcaga ggccatttat
     7561 ctgctggagc ggaaaaggtt atcaacagtg cgccatttaa aataaaggat aaatctaaaa
     7621 aaattccgga agatagcact acccttattt acggtataaa tcatatctcc tataaccaga
     7681 ataaacataa tattatttct gccgcctcct gtaccactac cgctttagca catatggtca
     7741 aacctttgct ggaaagggag gagaccaaaa atatattaac cgcatcatta tctaccatcc
     7801 atgctgcaac caatagtcag agtgtgctcg attcactgcc cggagctggt gatacagatt
     7861 tacgaaaaaa taggatggtc ttcaataata ttattctctc tactaccggg gctgccagta
     7921 cactggaaaa ggttttacct gaagtgaaaa gggttggttt tatggccgat tctgtacgca
     7981 ttcctactga caccgtttct ctgattattt tgaatcttac ctttcatagc actctggata
     8041 aaaaaggaga ctctactatt aatcgagagt ttattaatca aatataccat gatgcatcac
     8101 agggagcaca aagagaacta ttgtgttaca gtgataatca aaatgtatca tcagatttaa
     8161 agggaaaact ttctgcagtg accattgagg gacatgctac tcacacccgt accggtttta
     8221 ttggcttaaa cccgcagatg ttgcaagatg taggaatagt taccggtagg attattgata
     8281 tcccaataac tcatttaact gtatttggct ggtatgataa tgaatatggc agctatgttc
     8341 actgtttggg gaagctggct acctatatcc accggaattt gtagaccttt taataatggc
     8401 ttattatagt aagatttttt atgaaggagt ttagaattgt tagtttgcca atgaaaaaaa
     8461 taaatattca ccaaatgata ctggtctcac tgtttgcagc actgactgca gttggtgctt
     8521 ttatttcaat tcccatcggg ctggtgccca taagcctgca aaatctcttt actcttttat
     8581 ctggaatggt tttaggaagc agaatgggaa cttttagtca gctcatctat atcttactgg
     8641 gggtagcagg gttaccggtt ttctccggtt ttagggcagg tttaggtgtc atattgggac
     8701 caacaggcgg ttttttaacc ggttttgtta tttctactta tattgtaggc aaaatattag
     8761 aaaaaatagc agatgccagg gtatggtctt attttgttgc gggaatagtg gggataatca
     8821 taatttatac ctgtggaata atgcaattgc taatcgttac tcagataggg ttaaaagagg
     8881 ctgttctgat agggattgtt ccttttttac caggtgattt cataaaatta atcaccgctt
     8941 ctttcctggc tatcagaatc aaatctgtag tctccttacc acaggtatga tataatctgt
     9001 tttataatga ttcggggcgt agcgcagctt ggttagcgcg cttggttcgg gaccaagagg
     9061 tcggaggttc aaatcctctc gccccgacca tcaatctcct aataatttta tcgtttttat
     9121 tgcttaattt atgttaattt gttattatag tttataagag aaaaacgtta ctttgtatta
     9181 acatagaaaa tataataaat ttaatgattt tttggatttg agaaaaatat gtatcaaaca
     9241 aaaactttac ttacaattaa agaagcaagt caatgggcaa gtaattttct aaaaagagaa
     9301 attagtgagt ccaatatatc ttatttggtt caatatggga aagttagaaa atataatggt
     9361 ggtaattgtg cactaataga tttaaatgat ttaaaatatt actaccagtc ttttcgagga
     9421 tggcgtgaaa taagttggaa gagaaaactg ggagaagatt tgaactggaa tttgtctttt
     9481 gattatttaa aagaaaaaga taccacaaag catgtgcatc gtttgcaccc ttataaagga
     9541 aaatttattc cccagctcgt tcaatacttt attgataatc atacagatga ttttaagaaa
     9601 gatgtttatt ttaaaccagg tgatataata cttgaccctt ttttgggctc tggaactaca
     9661 cttgtacagg ctaatgaaat gggaatgcat tctataggag tcgatatatc acgttttaat
     9721 tgcttaatcg cagaaacaaa actacttgat tatgatttta tttctttaga aaaagaaatt
     9781 aaaaaaatac aaaaggctat tgttgaattt gaaactgaaa gcaatatcga aggttttgaa
     9841 ggagaactat tgtttaagtt agctgaattc aataaaaagt attttcccag tccagaattt
     9901 aagtacaaag taaataggaa acaaatagaa gaggggaaat acggtactga aaaggaacaa
     9961 gaatttttga gtatatata
//
LOCUS       DAOQWT010000006         9910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_7312_length_9910_cov_4.562253, whole genome shotgun sequence.
ACCESSION   DAOQWT010000006 DAOQWT010000000
VERSION     DAOQWT010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7312_length_9910_cov_4.562253"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKJ95_00365"
     CDS             <1..181
                     /locus_tag="PKJ95_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64726.1"
                     /translation="ANEMNTQTYNAKVNLSNNIFQSIPVSYNVKERLTINGSVNILPF
                     ASFTGLPFADAGGSL"
     gene            181..2409
                     /locus_tag="PKJ95_00370"
     CDS             181..2409
                     /locus_tag="PKJ95_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64727.1"
                     /translation="MGVVIRQIYDQAPETPPKRLFPIVRVKREDTTLNTVTNFSITKS
                     CENIGDSFSISFIGQDEFEYLHPLSGLAEYSIITGYIQGGEEKTVTQVVGVANTRSQK
                     LIWNSLNTSANFITGARKLVAPYAQRISVDVFLENRTAIQSWDKTLPLEQQITITTAS
                     ELIALILQDTGIDFVYSAYDYKISNYQRTGYPIEIIKDLTDFIGAYMIFDATNNRLEI
                     KEKNKFDGSHYDFLYVDNGDIVELGIENNSGDLVNCVLVKGVLPDDAPEYKDFYDKDK
                     EKEEEKKDDTIGDGYSPGTDDQLYLIDTIQGTKKAPDDPYRKEAPTDLIEEKFFNTLL
                     NFDIDPGSITVEGGSWDSKEKKYLNASFLGYGGFVPSSAVLTTDVNSDSSKLPMAGSL
                     SPGEGKVGFPWCYEENTDGDGKGEFKLEDQVYRGMTLKGKIVDELTKDAITGAAVELN
                     FALESNIWQYWKNTSSQIVVSFYMPSGTVGDILGYGWEDVTGEYEAKEAPADFPKTAI
                     SGRGPTSESEESESGIFIFTEIPISQYTVTVNKSGYDEGELDIEIDPVSFDFLKEGNE
                     NKDTNSKYKLLDTPYYVVVYGKKAAPISFGYPATGTDTGTGEDDKDTYTEDAKIEDIK
                     PNKNISIDLRCTRGITLAKGRLIYASPITDSRITSREIAIKAGSAVLFESLYNREIVR
                     LQLPHNPWLEIGQIIAVQSYVKGWTGSDIPLLFVEDITPSYSVSDGEEGIYDIVTGRR
                     KI"
     gene            2406..2774
                     /locus_tag="PKJ95_00375"
     CDS             2406..2774
                     /locus_tag="PKJ95_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64728.1"
                     /translation="MKEVIKRLTKDKKEKTDSIQSGTIITLVGQSMACDYYDIRVNGT
                     YYSNVPCPLPQNVQNQLKLEQPKGIQITQNRKILLQRKPMFSANESVQVIFEGGNKNT
                     IKISLENSIVKFEETEIVYI"
     gene            2803..5376
                     /locus_tag="PKJ95_00380"
     CDS             2803..5376
                     /locus_tag="PKJ95_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64729.1"
                     /translation="MQVVRLKAQNKANVGLNPKNNTIYLSEPSGGIPYVELSNITVND
                     WYKNSHIKTLSGIYTGGRPTSIKVRDHYPHSIGTDWCRLSNLLTIEADTTRINTARTE
                     EYIPITGEDNYTALKETAFWGAINTPLTVNDIEVISSMRYDTIEPYAPEEGSANYYGD
                     RMEAVGVPMPTTPSPWYYGTIDYDEQTWEVEITLNPFTMFEGTAYQQTHNYWKPYHVT
                     SYSVGLNYDPRLHAIEVDITSSYNGETIHGKRIYYIWHVPQAWALATKAPKVTITSGA
                     CSGPYGLHRCPGWTPTSGYDAGYTASGGTGLLELQGNDINYKNILIQLYPSVKKRYNN
                     LSNIYSICVDSKGNYHILNSPTAYSILTKYDANRNYIRDSNINKLGSSECIYYDGYIW
                     VGLYYIQQYNTDGVLLNSFGGYGTADGKFDSYPKLAAFNNYIYGTIPSKLQVFSSTGT
                     YITKYLPPTGYSFRDITIDEEGNFYFRLSKTGDTTNTYIYKYDNSFNFITSWPTIYSS
                     SVYGIYYRNGYLYVQNGTNIEKQNSVDGTIISSASITNKDNLFVDENDNIYILETSTI
                     NVLDNNYNSLYKIGDSNRNIKRNGNGIGITELSQLKKAYAVVLPNNFDPTNSGTYSKM
                     TEYTGVDFSNITMDSNGLLEFKNLYPTETEYYIYAMFDILPDGTYNTQNSWLSKDTGT
                     YCPSGTVGNRRNYRWFTRYPSCSQIPAGSATQYCNQLYSFDTYMTDGRSIIVSLDDFE
                     SGTDLDTATILYYWFNWLRVHLWTNGSDGGRTKFWVTYSDDGVKKNYNNINDACNMLS
                     MGMPVQIYYRNENYFLYNGVTINSGWDLGTINLINRTIKNGAYLTTGFGESHNVERIS
                     S"
     gene            5373..5711
                     /locus_tag="PKJ95_00385"
     CDS             5373..5711
                     /locus_tag="PKJ95_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64730.1"
                     /translation="MTQIDFDNFINFLKNNKIFPPSLLNDPVWQKNTQIFLSTFERFL
                     KTDQGQDYIKDLPNHTYVRVIEDGSIIPASNNNCIECPQAEDLIPKENTIKVKKVNPF
                     EQILRGEYKK"
     gene            5708..6229
                     /locus_tag="PKJ95_00390"
     CDS             5708..6229
                     /locus_tag="PKJ95_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64731.1"
                     /translation="MNLNFYDNLDEQIIHTLHFPAQPIGQTNPVEIELHLWNRKDKTC
                     NWTATDVEIFTRTATDLEIGDTDINGQEIVTEGLIEAKSYGVVGTGITDDGQTSFTPV
                     TETASMSIGDIPVNCARKIFFRLKSISGNSTRKALFLINILYNYLRKTVYKVVEHTSE
                     NLFIGEDFIGDFL"
     gene            6226..7101
                     /locus_tag="PKJ95_00395"
     CDS             6226..7101
                     /locus_tag="PKJ95_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64732.1"
                     /translation="MIGDTSNYPSSRDTLFNVYDENYTGYSISDEKKTINSYSATLDH
                     YAKEDSISISGFSEVKSPSVPSQGEFLVSDYAIPSTSRTGIDYNIGKTLTFHSTAEGN
                     SIKISYITPGDKITGALLNKLIDGINNIQEVLGTIGSGKNLDIGVYTTIAAWLSYFFT
                     VFQTHTHSGAAVTGSTRQLNEGSLADGSISNVHIKSTAAIAQSKLSMGILDYGVGVIP
                     TGTDKYVDITSAYWETSNKIVMLTRGADGQTDPGTVGTLGFDSELVTEDGFRVKFHTA
                     ESSLATAIPFTWILR"
     gene            7117..7473
                     /locus_tag="PKJ95_00400"
     CDS             7117..7473
                     /locus_tag="PKJ95_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64733.1"
                     /translation="MALIAGNGDLKIVQGSTWEQPIYFPSLVDLSVTGLDAELTISKC
                     TDEQIKLNTGNGKIVIDSVNNKLDCYLTWEETKRFTRDGTWKIELIYPAVDLVPEEKF
                     CYGTGQIIVKFDITEV"
     gene            7476..8078
                     /locus_tag="PKJ95_00405"
     CDS             7476..8078
                     /locus_tag="PKJ95_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64734.1"
                     /translation="MIIPNQIVKIETIETKITPKFPEIKPEISFPEDNVSIKIIDNVI
                     KPAITQSAISIKISEEPVKIEIPEGVKGDKGDKGDTGESGSLSSYTYTAGEILGGHRV
                     IIIDNNLAYYADNTNLSHVNKPIGISNNASDEGGEVTIVFYGEMEESSWNWDVDKPIW
                     ISTNGLLIQTPPETGFSCIIAFPIAQTKIFIEKQEILIIN"
     gene            8095..8604
                     /locus_tag="PKJ95_00410"
     CDS             8095..8604
                     /locus_tag="PKJ95_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64735.1"
                     /translation="MAGDKYLYNNSGRITEKASIQSSAGAGDAGKIPALDSAGKLDIT
                     MMPTGIGADTITAEASEALSAGDFINLYNSSGLKMRKADATTAGKEANGFVLSAVEST
                     ASGTVYRQGTNNQLSGMTVGARQYLTTTPGARSETAPSATGNVVQFLGIATSATEMFF
                     EERESMVLA"
     gene            8626..>9910
                     /locus_tag="PKJ95_00415"
     CDS             8626..>9910
                     /locus_tag="PKJ95_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64736.1"
                     /translation="MAQKVLYNNAGRTSEIALSPSSVDLGNVTNDLQIPKSIVTTKGD
                     IITATDNATPVRQGVGSNGQIIVGDSNLANGLGYKDPAFRNWVINGNFDIAQRGTSFT
                     NPPAYTYTLDRWRTNSQPDGGTPPNITHTQEKLTPGDVPGSYYFYRINVDGAGTSLGA
                     GSYYQLVTYIEHATRKLAGLNKKVTLSLLARSSIVNKKIGVLLAQDYGTGGSPSSQEL
                     KSGTNWTLTSEFAKYTHAFTTNTLVGKTFGTNNDDALVLVIYSMWGSSSQSYVGATTP
                     ETFVGAGNIDIAQVALYAGDVAYPFEAVPFDIELQRCMRYYEKSYNYDVYPGTAATDG
                     NSIFIPAGGNCWEGDYIFLETKYKVTKRIAGTPIIYDTAGTINKVTLSTNNTSATPNI
                     SGVVFSPTVNNFGCYGRTTNVAHRGIQYAWYVDAGF"
BASE COUNT         3487 a         1593 c         1967 g         2863 t
ORIGIN      
        1 ggcaaatgaa atgaacactc agacttataa tgcaaaagtt aatctgtcga ataatatatt
       61 tcaatctata ccggtaagct ataatgtaaa agaaagactt actattaacg ggtctgttaa
      121 tatcttacca ttcgcatctt ttaccggatt accatttgca gatgcaggag gttctttata
      181 atgggagtag taatcaggca gatttatgat caggccccgg aaactccacc gaaaagatta
      241 tttcctatag tcagggttaa acgggaagat actactttaa ataccgttac aaatttctct
      301 atcacaaaat cctgtgagaa tataggcgat tctttttcta tttcctttat tggtcaggat
      361 gagttcgaat acctccaccc gttatccggt cttgcagaat acagtattat taccggatat
      421 attcaaggtg gggaagagaa aactgttacc caggttgtcg gcgtggcaaa tacacgaagc
      481 cagaagctca tatggaacag tttaaatacc tcggcaaatt ttattacagg cgcgagaaag
      541 ttagttgctc cgtatgctca gagaatatca gtcgatgtat ttcttgagaa cagaaccgct
      601 atacagtcat gggataaaac tcttccttta gaacagcaga taactattac aactgcatca
      661 gaattgattg ctttaattct tcaggataca ggtattgact ttgtatattc tgcttatgat
      721 tataaaattt ccaattacca gaggacaggt tatcccattg aaataataaa ggatctcact
      781 gattttatag gggcatatat gatatttgat gccacgaata accgtctgga gattaaggaa
      841 aagaataaat ttgacggcag ccattacgac tttctgtatg tagataatgg agatattgta
      901 gaactcggaa tagaaaataa ttccggcgat ctggttaact gtgtcctggt aaaaggggtg
      961 ctgcctgatg atgcaccgga atataaagat ttttacgaca aagataaaga aaaagaggaa
     1021 gaaaaaaaag acgatactat aggcgatggg tacagtcccg gaacagacga tcagttatat
     1081 ttaatagata ccatacaggg aacaaaaaaa gctcctgatg atccatacag gaaagaagct
     1141 cctacggatt taattgaaga gaaattcttt aataccctgc ttaacttcga cattgaccct
     1201 ggttcaatta cagtcgaagg tggttcatgg gacagcaagg aaaagaaata tttaaacgcc
     1261 tctttcctgg ggtatggtgg ttttgttcct tcttctgccg tattaactac agatgttaat
     1321 tcagactcca gtaaactacc aatggcagga tctctttctc cgggagaagg taaggtagga
     1381 tttccctggt gttacgaaga gaatacagac ggtgatggaa aaggtgaatt caaacttgaa
     1441 gaccaggtat ataggggtat gactttaaaa ggcaagattg tagatgaact cacaaaagat
     1501 gctattacag gggcagccgt agagttaaat tttgctttag aaagtaatat atggcagtac
     1561 tggaagaaca cttcaagcca gattgtagta agtttttata tgccttccgg cacagtcgga
     1621 gatatacttg ggtatggatg ggaagatgta acaggagaat atgaagcaaa agaagctccg
     1681 gcagatttcc cgaaaacagc catttccggc agaggcccga cttccgaatc tgaagagtca
     1741 gaatccggga tatttatttt tactgaaata cctatatcac agtatacagt aacggtaaat
     1801 aaatcaggat atgatgaagg agaactggac atagagatag accctgtatc atttgatttt
     1861 cttaaagaag gcaatgagaa taaagataca aattcaaaat ataagcttct tgatactcct
     1921 tattatgtgg tagtttatgg aaagaaagca gcacctatat ctttcggata ccctgcaacc
     1981 ggcacagata caggcacagg agaagatgat aaagatacct atactgaaga tgctaaaata
     2041 gaagatataa aaccgaataa aaacatatca atagatttaa gatgtaccag aggtataact
     2101 ctggcaaaag gaagattaat ttacgcatca cctattacag actcaaggat aacttccaga
     2161 gagattgcta taaaggcagg aagtgcagta ttatttgaat ctttatataa cagggaaata
     2221 gtaagacttc aattaccaca taacccctgg cttgaaatag gacagattat agcagttcag
     2281 tcatacgtca aaggttggac aggcagtgat attcctttgt tatttgttga agatataaca
     2341 ccgtcttatt ccgtaagcga cggcgaagaa ggaatttatg atatagttac cggcaggagg
     2401 aagatatgaa agaagtaata aaaagattaa ctaaggacaa aaaagaaaaa acagactcta
     2461 tacagagtgg aactataata acccttgtag gtcagtctat ggcatgtgac tactatgata
     2521 taagagttaa cggaacttat tatagtaatg ttccatgccc ccttcctcag aatgtccaga
     2581 atcaattaaa actggaacag cccaaaggta tacagattac tcagaataga aagatacttt
     2641 tacagaggaa acctatgttc tctgcaaatg aaagcgttca ggtaatcttt gaaggcggta
     2701 ataaaaatac tataaagatc tccctggaga actctattgt aaaatttgaa gagactgaga
     2761 tagtttatat ataaggtata tataccggaa ccggaggata acatgcaggt agtaagatta
     2821 aaagcacaga ataaagcgaa tgtcggatta aatccgaaga ataatactat atatctatct
     2881 gaaccttctg gcggaattcc ctatgttgaa ctgtcaaata taaccgtaaa tgattggtat
     2941 aagaatagcc acattaaaac cctgtctggt atatataccg gaggaagacc tacttctatt
     3001 aaagtaaggg atcattatcc gcatagtata ggtactgatt ggtgcaggtt aagcaattta
     3061 ttaacaatag aagctgacac aacaagaatt aatactgcaa gaacggagga atatattcca
     3121 attacgggtg aagataatta taccgcatta aaagaaactg ccttttgggg agctataaat
     3181 accccattga cagtaaatga tattgaagta ataagtagta tgcggtatga tactattgaa
     3241 ccttatgcac ctgaagaagg atctgccaat tactatggtg atcgtatgga agccgttggc
     3301 gttcccatgc ctactacccc ttcaccctgg tattacggaa ctattgatta cgatgaacaa
     3361 acatgggaag ttgaaataac actaaatccc tttacaatgt ttgaaggaac tgcttatcaa
     3421 caaacacata attactggaa accctatcat gttacaagtt attcggtagg cttaaattac
     3481 gatccacgcc ttcatgctat tgaagtagat attacctcaa gttataacgg ggaaaccata
     3541 catggtaaaa gaatttatta tatttggcac gttccacagg catgggcatt ggcaacaaaa
     3601 gcacctaaag ttactattac ttcaggtgct tgttccgggc cgtatgggtt gcaccgttgc
     3661 cctggttgga ctcccacttc cgggtatgat gccggttata ctgcttcagg tggaacaggc
     3721 ttacttgaat tacagggaaa tgatataaat tataaaaata tattaattca attatatcct
     3781 tcagttaaaa agcgatataa taatctttca aatatttatt cgatttgcgt tgatagcaaa
     3841 gggaattatc atattttaaa ttcccctact gcctacagta ttctaactaa atatgatgca
     3901 aatcgtaatt atatcagaga ttctaatata aataaattag gcagttcaga atgtatttat
     3961 tatgatggat atatatgggt tggattatat tatattcagc aatataatac ggatggtgtt
     4021 ttgttaaatt cttttggcgg ttacggtact gctgatggaa aatttgacag ttatcccaaa
     4081 ttggctgcct ttaataatta tatttacggc acaattcctt ctaagcttca ggttttttct
     4141 tcaactggaa cctatattac aaaatattta ccccctacag gttattcctt cagggatatt
     4201 acaatagatg aagaaggaaa tttttacttt agattatcaa aaaccggtga tacgactaat
     4261 acatatattt ataaatacga taatagtttt aattttataa cgtcatggcc tacaatatat
     4321 tcttcttccg tatatggaat ttattataga aacggatatt tgtatgttca aaatggaacg
     4381 aacattgaaa aacaaaattc tgtagatggc actataattt caagtgcaag cataacgaat
     4441 aaagataatt tgtttgttga cgaaaacgac aatatttata tcttagaaac ttccacaata
     4501 aacgttttag acaataatta taattcattg tataagatag gtgatagcaa taggaatata
     4561 aaaagaaatg gtaatggtat tggaataaca gaattaagtc aacttaaaaa agcgtatgca
     4621 gttgtgttac caaacaattt cgatcccaca aattcaggaa cttattcaaa aatgactgaa
     4681 tacaccggag ttgattttag taatattaca atggacagta atggattatt ggaatttaaa
     4741 aacttatatc caactgaaac tgaatattat atttatgcta tgtttgatat tttaccggat
     4801 ggtacttata acacccaaaa tagttggtta tctaaagata ctggtactta ctgtccttct
     4861 ggcacagtcg gaaacagacg taattataga tggtttaccc gttatccgtc atgttcgcaa
     4921 atccctgcag gttcggcaac gcaatattgt aatcaattat atagtttcga tacgtatatg
     4981 actgatggca gatctattat tgtatcattg gatgattttg aatctggaac agatcttgat
     5041 actgctacaa tattatatta ttggtttaac tggttaaggg ttcacctgtg gacaaatgga
     5101 tctgatggag gtagaactaa attttgggtt acatattccg atgatggtgt taagaaaaat
     5161 tataacaata taaatgacgc ttgtaatatg ttatcaatgg gtatgcctgt tcagatttac
     5221 tatcgaaacg aaaattactt tttatataat ggcgtaacaa taaattccgg gtgggatttg
     5281 ggaacaataa atttaataaa tagaacgata aaaaatgggg cttatttaac cactggattc
     5341 ggagaatctc ataatgtaga aaggataagt tcatgacaca aattgacttt gataatttca
     5401 ttaatttttt aaaaaataat aaaatattcc caccttcatt attaaacgat cccgtatggc
     5461 aaaaaaacac acagatcttt ttaagtacgt ttgaaaggtt tcttaaaact gaccagggac
     5521 aggattacat aaaagactta ccaaatcata cttatgtcag agtaatagaa gatggaagta
     5581 ttattccggc aagtaataat aactgtatag aatgtccaca ggcagaagat ttaataccaa
     5641 aagaaaatac aataaaagta aagaaagtaa acccattcga acagatatta aggggtgagt
     5701 ataaaaaatg aatcttaatt tttacgacaa ccttgatgaa cagataattc atactttaca
     5761 tttccctgcg cagcccatag gacaaacaaa ccctgtagaa atagaattgc atttatggaa
     5821 cagaaaagat aagacatgta actggacagc tacagacgtt gaaatattca ccaggacagc
     5881 gacggattta gaaatagggg atactgacat taatggtcag gaaatagtta cagaggggct
     5941 aatcgaagca aaatcatatg gtgtggtagg gacaggcata accgatgatg gacagacctc
     6001 ctttacacct gttacggaaa cagcctctat gagtatagga gatatacctg taaactgtgc
     6061 gaggaaaata tttttccggt tgaaaagtat ttccggtaat tctacaagaa aggctttatt
     6121 tttgataaat atattgtata attacttaag gaaaacggta tataaagtgg tcgaacatac
     6181 aagcgaaaat ttatttatcg gtgaagattt tataggagat tttttatgat cggcgataca
     6241 tccaattacc caagttcaag agatactttg tttaatgttt acgatgaaaa ttatacaggg
     6301 tattccatat cagacgaaaa gaaaactata aacagttatt ctgctactct ggatcattac
     6361 gcaaaagaag acagtatttc catatccggc ttttcggaag ttaagtctcc ttccgtgccg
     6421 tctcagggtg aatttctggt aagtgattat gctatcccgt ctacaagcag aaccggaata
     6481 gattacaata taggaaaaac tcttactttc cattccacag cagaaggaaa ctccattaag
     6541 atctcttata tcacgccggg agataaaata accggggcct tgttaaataa actcatagac
     6601 ggaattaata atatacagga agttttaggc acaataggct ccgggaaaaa cctcgacata
     6661 ggagtatata caaccatcgc cgcatggtta tcttactttt ttacggtgtt tcagacccat
     6721 actcattccg gcgcggcagt tacaggttcc accagacagt taaatgaagg ttccctggca
     6781 gacggttcaa tatcaaatgt acatataaaa tcaacggcag ctatagcaca gagtaaatta
     6841 agcatgggaa ttttagatta cggcgttgga gttattccca ccggcacaga taaatatgta
     6901 gatatcacct ctgcatattg ggaaacttct aataaaatcg taatgcttac caggggcgca
     6961 gacggacaga ctgaccctgg tacagtcggg actctgggat ttgatagtga acttgttact
     7021 gaagacggtt ttcgcgtaaa attccacacg gcagagtctt ctctggcaac ggcaattcca
     7081 tttacatgga tattaagata aaaggaggtt tattttatgg ccctcatagc aggtaacgga
     7141 gatttaaaaa tagttcaggg ttcaacatgg gaacaaccaa tatatttccc ttcattggta
     7201 gatttgagcg ttacgggcct tgatgcagaa ttaactattt caaaatgcac tgatgaacag
     7261 ataaaattaa ataccggcaa tggtaaaata gtcatagact cggtaaacaa taaacttgac
     7321 tgttatctta cctgggaaga aacaaaaaga tttacgagag atggaacatg gaaaattgaa
     7381 ttaatatatc ctgcagttga tttagttcca gaagaaaaat tctgttatgg aacagggcaa
     7441 attatagtaa agtttgatat tacagaggtt taataatgat aataccaaat cagattgtaa
     7501 aaatagaaac gatagaaact aaaattactc cgaagttccc ggagataaaa cctgaaatat
     7561 ctttcccgga agataatgtt tctattaaaa ttattgacaa cgttataaaa cctgcaataa
     7621 cacaatctgc aataagtata aaaatatctg aagaacctgt taaaatagaa atcccggaag
     7681 gagtgaaggg agataaaggg gacaagggag ataccggaga aagtggatca ttatcttcat
     7741 acacttatac agcaggtgaa attcttggag gtcacagggt aataataata gataataatt
     7801 tagcttatta tgctgacaac acaaaccttt ctcatgtaaa taaacctata ggaataagta
     7861 ataatgcaag tgacgaaggt ggagaagtga cgatagtttt ttatggagaa atggaagaga
     7921 gttcttggaa ttgggatgta gataaaccta tatggatttc cactaatggt ttacttatac
     7981 aaactccacc cgaaacaggt ttttcttgta ttatagcttt tccaatagca caaacaaaaa
     8041 tatttattga aaaacaagaa atattaatta taaattaata aggagtgaat gattatggca
     8101 ggtgataaat atctttataa taacagtggg agaataactg aaaaagcttc tattcaatct
     8161 tcagcagggg caggagatgc aggaaaaatc cctgctcttg actcagcagg gaaacttgat
     8221 attactatga tgccaacagg cataggtgca gatactatta cagcagaagc aagcgaagct
     8281 ctttcagcag gagattttat aaatctttat aattcttctg gtttaaaaat gagaaaggct
     8341 gatgctacga cggcaggaaa agaagcaaac ggatttgttt tatctgctgt tgaaagtacg
     8401 gcttcgggaa cagtttatag acaaggcacg aataatcaac tctctggaat gactgtggga
     8461 gcaagacaat atctgactac tactccaggt gcaaggtctg aaacagcccc gtctgcaacg
     8521 ggaaatgttg tccaattttt aggaattgct acatctgcga ctgaaatgtt ttttgaagaa
     8581 agagaaagca tggttttagc ataatttaaa aaaggggaaa gtgatatggc acaaaaagtt
     8641 ttatacaata atgcaggaag aacgagtgaa atagcacttt ctccttcttc tgtagattta
     8701 ggtaatgtta ctaatgatct acaaatacca aaatctattg taacaacaaa aggtgacatt
     8761 attactgcga ctgacaacgc tactcctgta aggcaaggtg taggtagtaa cggtcagatt
     8821 atcgtaggag atagtaattt agctaatgga ttaggctata aagatcctgc atttagaaat
     8881 tgggttataa atggaaattt tgatattgct cagaggggaa caagttttac taatccacct
     8941 gcgtatacat atactttaga taggtggaga actaattctc aaccagacgg gggaacgccg
     9001 cctaatatta cccatacaca agaaaaatta actcctgggg atgtgccagg ttcatattat
     9061 ttctatagaa ttaatgtaga tggcgcggga acgtctttag gcgcagggtc ttactaccag
     9121 ttagttactt atattgaaca cgcaacaaga aaactggcgg gactaaataa aaaagtaact
     9181 ctttccctgt tggcaagaag ctctatagtt aataaaaaaa ttggagtttt actggctcaa
     9241 gattatggta caggtggaag tccgtcttct caggagttaa aaagcggaac aaattggact
     9301 ttaacttctg aatttgcaaa atatactcac gcattcacga ccaatacatt agttggtaaa
     9361 acttttggaa caaataatga tgatgcttta gttcttgtta tttattctat gtggggaagt
     9421 tcttctcagt catatgtcgg tgctactaca cccgaaacct tcgtcggtgc gggaaacatc
     9481 gacatagctc aagtggctct ttatgcagga gatgttgctt atccttttga ggctgttcct
     9541 tttgatatag aattacagag atgtatgagg tattatgaga aaagttataa ctatgatgta
     9601 tatcctggta ctgccgcaac tgatggaaac tctattttta ttcctgctgg cggtaattgt
     9661 tgggaaggtg attatatatt ccttgaaaca aaatataaag ttactaaaag aatagcgggg
     9721 actcctatta tatacgatac agccgggact ataaataaag taacgttgag tacgaataat
     9781 acatcagcaa ctccaaatat atcaggtgtt gtattttctc caacagtaaa taattttggg
     9841 tgctatggga gaacaactaa tgttgcacat agaggaatac agtatgcatg gtatgttgat
     9901 gcgggttttt
//
LOCUS       DAOQWT010000007         8773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_8478_length_8773_cov_3.309245, whole genome shotgun sequence.
ACCESSION   DAOQWT010000007 DAOQWT010000000
VERSION     DAOQWT010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8478_length_8773_cov_3.309245"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="PKJ95_00420"
     CDS             <1..1393
                     /locus_tag="PKJ95_00420"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNR64737.1"
                     /translation="NGTYGGVRGGRDFPLLDLSIANRTNKINIEVKIMGKKEIGIGII
                     GLGNVGTGTLEILTKNKDFIEQEISPNRIKIIGLADLDASRKPKTGNEYWFTHDAEDI
                     IKNSGLDILVETIGGEYPAYNFIKLALLKGKHVVTPNKEVVAKHGYELLEIAQQNRVQ
                     FLFETSVGSAIPILGVITNILTSCPLDEISGILNGTTNYILDLMAEQSMAQEDALKKA
                     QQMGYAEADPSKDVNGLDALFKIFILATLGFRGRLDLNKINYEGISGVTLDDTNLAKL
                     LNYRIKLIATARRIINKLEIGLGPVLIEEGHLLSHIRGADNGIFVSGQGYGNLFLSGP
                     GAGGIAAASMMVSDIVRIIRQPGCFDYDFLTNQKEELEISLPGETKSPYFLRIKGEDD
                     SERNINDIKKIIAEKGFLTDRSAVFKDKNNSLSMGFIISSGREREMIEIIQELKKLDY
                     VANPAYLKVYEHL"
     gene            1494..2387
                     /gene="thrB"
                     /locus_tag="PKJ95_00425"
                     /pseudo
     CDS             1494..2387
                     /gene="thrB"
                     /locus_tag="PKJ95_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
     gene            2418..3638
                     /locus_tag="PKJ95_00430"
     CDS             2418..3638
                     /locus_tag="PKJ95_00430"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNR64738.1"
                     /translation="MIVQKYGGSSVADIHKIKMIAGKIVEQARLGNRMIVVVSAMGKT
                     TDDLINKARQIITNPSEREMDMLLSTGEQISVALVAIAIQSLGVEAISFTGAQAGIRT
                     NNSFSNARINGINEQRIQKALKQGKIVIVAGFQGIDDDGNITTLGRGGSDTTAIALAA
                     RLKAERCEIYTDVDGIFTADPGLVPEARKIKEISHDEIAEMSILGAKVLYYRAIDIAR
                     NYGVQVLVKSSFGNGSGTLIRTKEEISMLEKVNVRAVAHEINAGKIIIKNVPDIPGIA
                     ARLFRALAKDEIMVDMIIQSIEHNQVNDIAFTVAKNNLAKALEITQKFAADIGSPEVL
                     ADDEVAKISIVGAGITSDPEIAARMFEALARNDINIDMISTSGTRISCLIHKKHIETA
                     VKAVHQEFQLEEEN"
     gene            3641..4642
                     /locus_tag="PKJ95_00435"
     CDS             3641..4642
                     /locus_tag="PKJ95_00435"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNR64739.1"
                     /translation="MKQYNVAVVGATGAVGEEMTRILEERKFPINQLFLFASHRSAGK
                     EYYFRNERVEVRELKDSSFQGIDIALFSGGDEVSKHFAPLAVKAGTVVIDNGKYYRMD
                     PNVPLIIPEVNPEDVGEHQGIIANPNCSTTQMVVALKPIYDAVGIKNVICATYQSVSG
                     TGKEAIEELKEQSKAFLEDGNINIEVYPHQIAFNVLPHIGSFDETGYSSEEIKMLNET
                     RKILHDERINVSATTVRVPVFRAHAEDIHIETREEITPEQARKLLTKFPGICVIDQPG
                     ETKYPLSLMAEGKDEVFVGRIRKDLVFNPGLVMWVVSDNLIKGAALNTIQIAELLIK"
     gene            4873..5856
                     /gene="speB"
                     /locus_tag="PKJ95_00440"
     CDS             4873..5856
                     /gene="speB"
                     /locus_tag="PKJ95_00440"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNR64740.1"
                     /translation="MKDENIFNPKQVNMEFKGIASFGRSKMVNINDKWISDFTIIGIP
                     YDAGVAHIPGSRFAPREIRDVSTRYSFFGNEGSNGYWSIESKEYYLKKSIVVDAGDTD
                     VLCLDTQYCYAQIASSIEKILENNSIPVIIGGDHSISFPVLEGFAKKYKTIGIIHLDA
                     HLDYRNEFCGVNYSHGSPLRRICELPQISGALAIGCRGKLLLEEDYKDVLNRGIKVIT
                     AKDIRKLNSLAIEEIRKLLPQDHPVYVSIDIDFLDPSIAPGTGVCEPGGFDYPFLMEV
                     LEHISLQCKVVGFDLVEVNPMVDLSKMTSRIASEIIIGFMAYIWKQKEFKK"
     gene            5980..6726
                     /locus_tag="PKJ95_00445"
     CDS             5980..6726
                     /locus_tag="PKJ95_00445"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNR64741.1"
                     /translation="MLGNMLFFVFGFILALVLLLILWQLFLRPRGSGKDTAIYSSIEQ
                     IRSVGELVAFKMVTKEIVTASQHWLGDIGKRYFQWFVSTKKMAMVFEFDIDFRYNLRS
                     PDFRIDQIGGNSFILRMPPCLYQVHIRDISFYDEQNSKLLPWLLPDLLNRAFGSGFSE
                     ADKNQLKEEAKNQAAKMAEGVVNRLLSEVENSARHTLLGLAKGLQVEKIAIDFSQSGV
                     IPSTVQTNELEANIKKAKEGINNLVGKKEE"
     gene            6842..7054
                     /locus_tag="PKJ95_00450"
     CDS             6842..7054
                     /locus_tag="PKJ95_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012325715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNR64742.1"
                     /translation="MKISARNMLKGKIKAITEGTVNDEIIIELPGGMEITSVITRSSV
                     QNLALVVGKEVYAVIKSSNVMIAVDE"
     gene            complement(7065..7796)
                     /locus_tag="PKJ95_00455"
     CDS             complement(7065..7796)
                     /locus_tag="PKJ95_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64743.1"
                     /translation="MMDISKETLQKMKRESISPKPRWYFLTKNYFFWLMFALTTILGG
                     FAFGMIMFFTGDLDWDIYHYLGISLSRAILMSLPYLWIAFMLFFLFITYYNFIHTRTG
                     YRYQFILVFFISLLLSALLGLGFYQYGWTETVDRQLRTRIPGYGQLVHSKEKQWMHPE
                     RGLLAGTIEVIIIENNLLLLKDTFDKEWKIDISQVRVKGMLPLTRNLRIRILGQQISK
                     DTFKATEIRIPRGSPQSKGRKAAQP"
     gene            complement(7780..8379)
                     /locus_tag="PKJ95_00460"
     CDS             complement(7780..8379)
                     /locus_tag="PKJ95_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR64744.1"
                     /translation="MIKKFDGKQKTDAQLVTLTLKNQEYYYYLMKRYESALANYIQRL
                     SGMNRMDIQDILQEVFILAYQNLNDYNKNFKFSSWIYRIARNYTISVMRKNPKYFQNI
                     SWDEHDLEQLIKSDFNLEDSLIQKIDYENLLAIINLLPLKYKEVLLLKFVEGKDYQEI
                     GDILRKPIGTVGTLINRARKILLKEIEKNRAGEKYDGHK"
BASE COUNT         2893 a         1410 c         1868 g         2602 t
ORIGIN      
        1 gaacggcacg tacggtggtg tgagaggggg aagagatttc cccctactcg atttatcaat
       61 tgcaaataga acaaataaaa taaatattga ggtaaagata atgggtaaga aagagattgg
      121 aataggaatt attggattag gcaatgtagg tacaggaacc ctggaaatat taaccaaaaa
      181 taaagatttt atcgaacagg aaatttcacc caatagaata aaaataattg gtcttgccga
      241 tttagatgcc agcagaaagc caaaaacagg taatgaatat tggtttaccc atgatgctga
      301 agatatcatt aaaaattctg gcctggatat tctagttgag accattggcg gagaatatcc
      361 agcctacaat ttcattaaac tggcattatt gaaagggaaa catgttgtta cacctaacaa
      421 agaagtagta gcaaagcatg gatatgaact gctggagatt gctcaacaga atagagtcca
      481 attcttattt gaaacctctg tggggagtgc cattcctatt ttaggggtta ttaccaatat
      541 tctcacatcc tgccctttag atgaaatcag cggcatcctg aatggtacta ccaattatat
      601 tttggattta atggcagagc agagtatggc acaggaagat gctttaaaaa aggcccagca
      661 aatgggctat gctgaggctg accccagcaa agatgttaac ggactggacg ctctctttaa
      721 gatatttatt ttagctaccc ttggtttcag aggaagattg gacttaaaca agattaatta
      781 cgaaggtatt agtggagtga ctctcgatga tactaatcta gccaaattat taaattatcg
      841 tataaaacta attgctactg cacgaagaat tataaataaa ctggaaatag gactcggtcc
      901 agttttaatt gaagaaggac atctcttatc acatattcgt ggtgccgata atggcatttt
      961 cgtatcagga cagggttatg gaaatttatt tttgtccgga cctggcgcag gaggtattgc
     1021 agctgccagt atgatggtaa gcgatattgt caggataata agacaaccgg ggtgttttga
     1081 ttatgatttt ttaaccaacc agaaagaaga attggagata agtttaccag gtgagaccaa
     1141 aagtccatat tttttaagaa taaagggcga ggatgatagc gaaagaaata taaacgatat
     1201 taaaaaaatt atagctgaaa aaggtttctt aacagatagg tctgctgtgt tcaaagataa
     1261 gaataattcc ctctctatgg gttttataat ctcttctggc agagaacgtg agatgataga
     1321 gataatccag gagttaaaaa aattagatta tgtagcaaat ccggcatatc tgaaagtata
     1381 tgaacatctt taatttttta atttctgaaa aagagataaa tttaataatt tctattgaaa
     1441 tatttggtca attatgattt tttctgaaaa ttaatactaa aaggagaaaa acaatggtta
     1501 aagttaagat acctgctacc accgcaaatt taggttcagg ttttgattgc ctgggaatgg
     1561 ctttatccct ttacctggag atagaaatgg agagaatagg gcgaggtttt gaatttcagg
     1621 ctgaaggaga aggagttgaa aatttatctg ctgacaagaa caacctgata tatagagcag
     1681 caaccctggt aatggataaa ggtgatattg ctccgggaga gagaggaata aagatttcca
     1741 taaagaatga aattccatta gcaagaggat taggaagtag tgcagctgct attatcggtg
     1801 gaatagtagg ggcaagtgag ctatataact taaatttaag cagggaagaa atattaaaat
     1861 tggcttttaa attagaaggg catctggaca atattgtgcc agccctcatt ggaggattta
     1921 cgatttctta tagaggtcag aaaggggaaa taaggtgggt aaaattagat ttaccgcatg
     1981 acttgagagc agttgtaggg ataccttctt ttaccttaag tacagaagag atgaggaaag
     2041 tactacccaa acaggtttct cttcgggatg ctgtagataa tctgagtaaa tctgccctat
     2101 tggtaaatgc tttgcagcaa tcaaagtggg ggcttattcc agaggcaatg caggaccgtc
     2161 tgcaccagcc ctttcgtctt ccctttatta aaggggccag aaatatcttt accgaggtag
     2221 agaagagtgg tattgccggg gtagccttaa gcggttcagg acctactata atttcactgg
     2281 ttaaagaaga cctggaaaaa gagattatta agattatgaa aagaactttt tccgatgctg
     2341 gcattaagag tctggttaag gtgctggaac ctgatacaga tggggtggca gtaataaaag
     2401 gagaaaaaga ttaccccatc attgtacaga aatacggggg aagttctgtt gccgatattc
     2461 acaaaatcaa aatgattgcc ggcaagatcg tagagcaagc caggttgggt aaccgcatga
     2521 ttgtcgttgt ctcagcaatg ggtaagacga cagatgacct gatcaacaaa gcccggcaaa
     2581 taattacaaa tcccagtgaa agagagatgg atatgctctt gtctactggc gaacagatat
     2641 ctgtcgcctt agtagcaata gctatccaat ctcttggagt agaggctatt tcttttaccg
     2701 gagcacaggc gggcattagg accaacaatt ctttcagcaa tgccaggatt aatggtataa
     2761 atgaacaacg gatacaaaaa gctttgaagc agggaaagat tgtcattgtt gccggatttc
     2821 agggtattga tgacgatggg aatattacca cgctgggaag aggaggctct gatactacag
     2881 cgattgctct ggcggccaga ttgaaagccg agcgttgcga aatctatact gatgtagatg
     2941 gaattttcac agctgacccc gggttggtac cggaggccag gaagataaag gaaatttctc
     3001 atgatgaaat agctgaaatg tctattttag gagccaaagt attatactat cgggcaattg
     3061 atattgctcg aaattatggc gttcaggtgc tggtaaaatc aagctttgga aatggttcag
     3121 gtacattgat tagaacaaag gaggaaatat caatgttgga gaaagtaaat gtaagagcag
     3181 tagctcacga aattaatgcg ggaaaaataa taataaaaaa tgttccggat ataccgggaa
     3241 ttgctgccag gttatttaga gctttagcta aggatgagat tatggttgat atgattattc
     3301 agagtattga acataatcag gttaatgata ttgcttttac agtggcaaaa aacaatctgg
     3361 caaaggcact tgagataact cagaaatttg cagcagatat cggttctccc gaggtgcttg
     3421 ccgatgatga ggtggccaag atatcaatag tgggggcagg tattacctca gaccctgaaa
     3481 ttgctgcccg tatgtttgaa gccctggcgc gaaatgacat aaatattgat atgattagca
     3541 cttcggggac aagaatatcc tgtctgattc ataaaaaaca tattgagaca gcagtaaaag
     3601 ctgttcatca ggaatttcaa ttagaggagg aaaattgaaa atgaaacaat acaatgtagc
     3661 agtagtagga gcaactggag cggtagggga agagatgacc agaattttgg aagagagaaa
     3721 atttcccata aaccaacttt tcttatttgc ctctcatcgt tctgcgggga aagaatacta
     3781 tttccgaaat gaaagagttg aagttagaga gctgaaagat agttcttttc agggaatcga
     3841 tattgccctt ttttcgggag gagatgaggt aagcaaacat tttgctcccc ttgctgtaaa
     3901 ggctggaaca gtggttatcg ataatggcaa atattataga atggacccca atgtgccgtt
     3961 gatcatccct gaagttaacc ctgaggatgt tggggagcat cagggcatta ttgccaatcc
     4021 caattgttct actacacaga tggtagtggc gctaaagcca atttatgatg cagttggaat
     4081 taaaaatgtt atctgtgcta cctatcaatc agtttctgga acaggtaaag aggcgataga
     4141 agaattaaaa gaacagagca aggctttcct ggaagatggc aatatcaata ttgaggttta
     4201 tcctcaccag attgccttca atgttctgcc acatattggc tcttttgatg agaccggcta
     4261 ttcttctgag gagattaaaa tgctcaatga aaccagaaag atacttcatg atgagcgaat
     4321 taacgtgtcg gcaaccacgg taagggttcc ggttttccgc gcccatgccg aagatataca
     4381 tattgaaacc agagaagaga taactcctga acaagccaga aaattattga ctaaatttcc
     4441 cggcatatgc gtaattgacc agcctggaga aacaaaatat ccactttctc taatggcgga
     4501 aggtaaagat gaagtgttcg tgggacgcat aagaaaagat ctggtattca atccgggatt
     4561 agttatgtgg gttgtgtcag ataatcttat aaaaggtgct gccctaaata ctattcaaat
     4621 tgccgaacta ttgataaaat aataaatata ctattgcctt aacgattaaa taaagtagag
     4681 tctataaatg aacaccgatt aaataaaaaa acagacctgc tctctcatag aataattgtt
     4741 agattacaag aatagggata ccttgatttt ttacgaaaag ctttgtatta ccaaattatt
     4801 tttattgaat aaatactttt aaaatcatac caactagata aaaactatta ctaaaaagaa
     4861 aaggatagaa aaatgaaaga tgaaaacatt ttcaatccga aacaggttaa tatggagttc
     4921 aaagggatag catcttttgg cagaagcaaa atggtaaaca tcaatgataa gtggatttct
     4981 gattttacca ttattggtat tccctatgat gccggggtag cacatatacc gggctcccgg
     5041 tttgccccaa gagaaatcag ggatgtatca acaagatatt cattttttgg gaatgaaggc
     5101 tctaacgggt attggagtat agaaagcaaa gagtattacc tgaaaaaatc cattgtcgtg
     5161 gatgccggag atactgatgt cttgtgtttg gatacccaat attgctatgc acaaatagca
     5221 agttctattg aaaaaatatt ggaaaataat tctattccgg taattattgg aggagatcat
     5281 tcgattagct tccctgttct ggaaggattt gcaaagaagt ataaaacgat cggtattatt
     5341 catttagatg cacatctgga ctataggaat gaattttgtg gtgtaaatta ttctcatggg
     5401 agtcccttaa gaagaatttg cgagctgccg caaatcagtg gtgcattggc aattggttgc
     5461 agagggaaac ttcttttaga agaagattat aaagatgttc tgaatcgagg gataaaggtt
     5521 attactgcta aagatatcag aaaattaaac agtttggcaa tagaagaaat aagaaagctt
     5581 ttaccacaag accatccagt ttatgttagt atagatatcg atttcctaga tccttccatt
     5641 gctccgggga ctggggtttg tgagccagga ggtttcgatt atcctttttt aatggaagta
     5701 ttagagcaca tatcactaca atgcaaggtg gtgggatttg atcttgtgga agtcaatcct
     5761 atggtcgatc tctccaaaat gacatcacga attgcctctg aaattatcat aggatttatg
     5821 gcctatatct ggaaacaaaa agaatttaaa aagtaaacat cctatcctgt actatgggtt
     5881 gatatttcca ttggttaatc ctataataac tatctgctca tttcacttat catcaattgc
     5941 ttaaaaggaa aatattttaa agagaagggg aattgcaaaa tgctgggcaa tatgctcttt
     6001 tttgtttttg gatttatcct ggctcttgtt ctgttattga ttttatggca gctatttctc
     6061 cgtcctcgtg gttcggggaa agataccgcc atatattctt caattgaaca gattcgttca
     6121 gtaggggagc tggtggcttt taagatggta acgaaagaga ttgttaccgc ttctcaacac
     6181 tggctgggtg atatcgggaa gaggtacttt caatggtttg tctctaccaa aaagatggcc
     6241 atggtttttg agtttgatat tgattttcgt tacaatttac gcagccctga ttttagaatt
     6301 gatcagatag gcggaaatag cttcatttta agaatgcccc cctgtcttta tcaggtgcat
     6361 atcagagata ttagttttta tgatgaacag aattcaaaac tcctgccctg gttattgcct
     6421 gatctgctta atcgtgcttt tgggtctggt tttagtgaag cagataagaa tcaattaaaa
     6481 gaggaagcaa aaaatcaggc tgctaaaatg gcggaagggg tagttaatag attattatca
     6541 gaggtggaaa attcagcccg gcataccctg ctgggattag ccaaaggctt gcaggtagag
     6601 aaaattgcca ttgatttcag ccagtcaggt gttattccca gtacagttca aaccaatgaa
     6661 ctggaagcga atataaaaaa agcaaaagag ggaataaaca atctggtggg taaaaaagaa
     6721 gaataaagtc atctcatatc tatagttaat aagatttggt tgccataatg tttagctggg
     6781 atatttaaaa atagtctata aatatgaaaa aaagaatata aacttgaaag gagtcagcgg
     6841 tatgaaaata agtgcaagaa atatgctgaa aggtaaaata aaagctataa ctgaagggac
     6901 agttaatgac gagattatca ttgagctacc tggcggtatg gagatcacct ctgttattac
     6961 cagatcttct gtccaaaatt tggccttagt tgttggcaaa gaagtttatg ccgtaatcaa
     7021 atcaagcaat gttatgatag ctgtggatga atagtaataa atattcaggg ttgggccgct
     7081 ttcctacctt tggactgggg tgaacccctg ggaattctga tttcggttgc cttaaaggta
     7141 tctttggata tttgttgtcc caatatcctt attcttaaat tcctggttaa aggtagcatt
     7201 ccttttactc taacctggct aatatcaatt ttccactctt tgtcgaaagt gtcttttaag
     7261 agcaacaggt tattttctat tatgattacc tcaatcgttc ccgctaataa acccctttca
     7321 gggtgcatcc attgtttttc tttgctgtga accagctggc cataacctgg aatcctagtt
     7381 cttaactgcc tatcaacagt ttcggtccag ccatattgat agaatcccaa tcccaataag
     7441 gcactcagaa gcaggctaat gaagaatacc aatataaatt gatacctata accggttcgg
     7501 gtatggataa agttataata ggtgataaac aaaaagaaga gcataaaagc aatccagaga
     7561 tagggcaagc tcataagtat tgccctggat aggctgatac ccagataatg ataaatatcc
     7621 cagtccagat ccccggtaaa aaacattatc atcccaaaag caaatccacc cagtatagtg
     7681 gttaaagcaa acattaacca gaaaaagtaa ttcttagtta aaaaatacca gcgaggtttg
     7741 ggggatatac tttctctttt cattttctgc aaagtttctt tacttatgtc catcatattt
     7801 ttcacctgct ctgttttttt caatttcttt taataaaatc ttcctggcac gattgataag
     7861 agttcccacc gtacctatgg gttttcttaa gatatcacca atttcctgat aatcctttcc
     7921 ttctacaaac tttaacagca gtacttcttt atatttcaag ggtagtaagt tgataatggc
     7981 aagtaaattt tcatagtcta tcttttgtat caaactatct tccaggttaa aatctgattt
     8041 tatcagctgc tccaaatcat gttcatccca ggagatgttt tggaagtatt ttgggttttt
     8101 acgcatcaca ctaatagtat aattacgggc aatgcggtaa atccagctgg aaaatttaaa
     8161 atttttattg taatcattca aattctggta ggccagaata aaaacttcct gtaaaatatc
     8221 ctgaatatcc attctgttca ttcctgatag tctttgaata taattcgcaa gggcagattc
     8281 atatcttttc ataagatagt agtaatattc ctgatttttt aaagtcaggg tgaccaattg
     8341 agcatctgtt ttttgttttc catcaaattt ttttatcata aaaatattgc ttcttttttc
     8401 ctctttacca actcaattcg gaagaacaaa atgataagaa gcttagttta ccactctctc
     8461 atattataat ataaagggtt atggccgtgt ttgctaaaat atttttgtct atcaaatata
     8521 atacgattaa atctttgttt ttctttcaaa atattttcta taaatagttt tatggaaaag
     8581 aaatttttgg gaagtgtggt gaaggaataa acaaaaaaga gaagaatgaa aaaattatag
     8641 gggataatta ttctttatgg gcaagcttct aatgccctct aataaaactc tcatagcaat
     8701 acaatcatct tcattgtatt gcagaatacg ttctttgatt tgcagccttt cctgttctaa
     8761 ccagcggtgg tac
//
LOCUS       DAOQWT010000008         8159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_9278_length_8159_cov_3.790104, whole genome shotgun sequence.
ACCESSION   DAOQWT010000008 DAOQWT010000000
VERSION     DAOQWT010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9278_length_8159_cov_3.790104"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PKJ95_00465"
     CDS             complement(<1..666)
                     /locus_tag="PKJ95_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64745.1"
                     /translation="MSILDGSVGGSTATINDYSRLDNVRGTILPVITELTMGHRVAAF
                     NGQHIFPVVTIPKMKATIPKFGKEAFKRYETLRGMGGDSNVLVRDKRDTIQVATEEHD
                     AAYPIDYLESQEANFNLQKWAGFRAKRAVELEMEMQIAGIVQNLDNYSSTNKITLGSG
                     QNPAKWSDYITAPTTYTSDPQNDVEVAKEAVAAAVGIEPNCLFMGLNVFKQARRHPIF
                     KELI"
     gene            complement(671..2635)
                     /locus_tag="PKJ95_00470"
     CDS             complement(671..2635)
                     /locus_tag="PKJ95_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64746.1"
                     /translation="MLGSITDHNGVELENRIEKKENPDLNGKWIEVFEGGVQTDSEGR
                     TDVFSEDKLDYTVKSYNPTHHEAPLCLGHPPEHNSPAYGWVEAIKRTGNKLLAKFKQV
                     PPEISNAIHSGFWKKRSISFYPDGRLRHVGFLGAQPPAVKGLADIKFKEKEEEYVTFS
                     QSNDIAKTISEKPPDNFSRMDNLNPTISIVNDLKIDKPDKVVKENKDLWPENTDNLSE
                     TPGEIRHKIRSPDMFDIMTRMVELKSDPGILIHLGKLPGDDTETSHAVTFVKEKGWTM
                     DKAKQWMNDHKNKYPLHGNVANFAETLSHTETHCADIKKYCDTINNTCNNSRMSKEDI
                     STIETCCATITQYCDLMGSMGIAKCCDIIRDSSKAIRSLCNDYPDSNIIKSCCEAIDK
                     SCDKLRGNDYYFNEEIKPAEEAEKDISPEKDYKEVKGMKKKKVEGETPEEETTETPEE
                     ETTEEVSNGKKKENSGCKSKKGKAFSEYSEEEIAELEQKASRITFLEQKAEEEKKARE
                     FAEAELKKIATEKKLAEYKSFCEKELGDKLLPADHSDVIDLLDFCETHGSYNFAEGGE
                     QKTVEKFKSLLKKYIPEHKLFTEVATNKIANQQLSPIKGKNTKVRQFAEVKNMTVDRE
                     QEIDDQRTRQYMSQHPGVKYEQALKAIKEV"
     gene            2778..4304
                     /locus_tag="PKJ95_00475"
     CDS             2778..4304
                     /locus_tag="PKJ95_00475"
                     /inference="COORDINATES: protein motif:HMM:NF017850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HNR64747.1"
                     /translation="MAKLWINEQNYINIDKKQPLFSQEIATRKRSINFMSFLGYLPNP
                     DAVLKRTGQNIKVYEELRTEPQTRTCIQSRKSGTMSLLHGINRGKAKTEHSQFIEENF
                     SQLPIQTIIEQILDAPAYGFQPLEVTYGTLPSGKMEAKKVEAKPVEWFVFDDENNLRF
                     RSKWDYMIGELLPDKKFLCPSYEATYNNPYGNPLLSSCFWPITFKKGGLQFWVVFVEK
                     YGMPHVIGKVPPGTKQDDRGRVMEMLDAMVQDAVAVINNDETIEFVSDTGKSASSNTF
                     SQLINYCNDDISKVILGQTLTTQVGNVGSYAAGKVHATIRQDIVDTDVKLVKQTFNQL
                     IRWITEINWGKVNDYPTFDMWAEEDIDLAIAQRDKTLVDAGLKFSKKYWQKTYGFEAD
                     DFELIPSSPPSLTPSSPPIQFTEEGNELKEDNKLSSEFSESQDIEDRFIQDASLHLIG
                     EKFVDGIVKYIKSCKSFDEAKKGLYKQYENIDQGLFQEVMEKALFYSDIAGRTSDITK
                     "
     gene            4315..6144
                     /locus_tag="PKJ95_00480"
     CDS             4315..6144
                     /locus_tag="PKJ95_00480"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HNR64748.1"
                     /translation="MFTKKDINRIYKIASYKRKQALKNYAEKAEQLTLNFGDLKMLFG
                     LPPKEAIEYFKKKKPELTEKYYDLWKDAHDKSFTIAGIASLSLLTDIQGKLQQALKEG
                     MTYKQFEEEFESSVLKKMGWTSDDEDIPPYRIENIFRTNIGGAYCAGRWQSIEDNKES
                     RPYLMYHTMEDPQVRTPHADMDKKVFRVDDPIWDTHAAKNGYGCRCWMQALSERDIKR
                     KGIKVEDSGGRIIEKEIVLNKAEDIRVKISVYIDPKGNHIPVDPGFDYNPGKKNYIPD
                     PKNFPPALRPIAEKLIENIPEVKENINSVWENGVLIPERISKWQESNEDERKILLNEV
                     LSKKLQEDLKTPLDLPNGYIYGENTKEFDKYYGRESNGYVPAYYSNSEGRIVFNLSVM
                     EDNYRYIVPLHENTHVFNKKQFNRMGDTFYSEGSTDLFAMRKYVEYTGKEVYISDYGN
                     MCSDSMIQLIKEFKGNKSEILNFLKNVILGKKEDIEKYREVVLKTKDIYHNYMTQQEY
                     NEMLKDLIKTEEIDPVIKKAIKTYLKDFGKKEMLKKILTSEQIDKEVNYAKEKLNYSQ
                     EEQDALRRCYEDQNRNLKNYNKGDINHSDDLRKEVLEWWTKID"
     gene            6123..6299
                     /locus_tag="PKJ95_00485"
     CDS             6123..6299
                     /locus_tag="PKJ95_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64749.1"
                     /translation="MVDKDRLNILINAFYSEKKDIKKLEMLRNLILKIDNSYSMVLSR
                     DIENFKGLPEGVVI"
     gene            6299..6745
                     /locus_tag="PKJ95_00490"
     CDS             6299..6745
                     /locus_tag="PKJ95_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HNR64750.1"
                     /translation="MVEITVEDRELQSELNSLKERYQDLSPLMLTLSEDLVFFITENF
                     ETEGKRLPPDKQWPELSDWTINEREYKGYWPGKKLHQTGILVNSIHSEYSSSEAVAGT
                     NVPYAEINHFGGINDGGFYVPPRPFMELIDQDLEDMMEDVMSYLGK"
     gene            complement(7044..7592)
                     /locus_tag="PKJ95_00495"
     CDS             complement(7044..7592)
                     /locus_tag="PKJ95_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64751.1"
                     /translation="MSIPPSKSLAINITLKKDKPVIELASRKNPTINFYDLVKKIERE
                     MFKSIIEPLYLRGGERKTMEVMYSPTWEEFLDTFPTKREYVSRLQEELREINNRYRMG
                     FTEEEAKILQKREKDLKYCYDIIINEWKLISRPCIKFDSEEEGIRYMIKEKMVDLKET
                     DIVIHKKGDKVEERDIEIIKEI"
     gene            complement(7565..>8159)
                     /locus_tag="PKJ95_00500"
     CDS             complement(7565..>8159)
                     /locus_tag="PKJ95_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64752.1"
                     /translation="KNPQRQWENEIADIISKVMRISKKEAEIEIKLALSEARLEYPEK
                     LQNIPINKTKQLNLRLTQNEMNYENYVKCVVSENNIFRPVIEERTYNIAQCAFECGVK
                     EIIRYKTENMKSEVIETSEGNVLAVINLSNYEINYSNLFQEYRKQLEIREQEIPDPPV
                     KECSSCGADAYELKNKHYYCKYCEGKYKICQFHHQKV"
BASE COUNT         2643 a         1408 c         1363 g         2745 t
ORIGIN      
        1 gataagttct ttgaatatcg gatgccttct tgcctgttta aacacattga gtcccataaa
       61 caaacagttc ggctctatac ctactgcggc ggcaactgct tctttcgcca cttcaacatc
      121 attctgcgga tctgatgtat aggtggttgg tgcagttatg taatcagacc attttgccgg
      181 gttctgtcca gagccgagag taattttatt ggtagatgaa tagttatcta aattctgaac
      241 tatacctgca atctgcattt ccatttctaa ctctactgct cttttagctc taaatccagc
      301 ccatttctgc aaattaaagt ttgcctcctg agattcgaga taatctatag gataagcggc
      361 atcgtgttcc tctgtcgcta cctgtatggt atctctttta tctcttacga ggacattact
      421 atctccaccc atacctctca gggtttcata tcttttgaaa gcctctttcc cgaacttggg
      481 tatagttgct ttcatctttg gaatagttac tacgggaaat atatgctgac cattaaaggc
      541 cgctactcta tgccccatag tcaattctgt tattaccggc aaaatagttc ctcttacatt
      601 atcaagtctt gaatagtcat ttattgttgc cgtagaaccg ccaacacttc catcaagtat
      661 gctcattaat ttatacctcc tttatggctt ttaaagcctg ttcgtatttt acgcctgggt
      721 gttgggacat atactgacgg gttctctgat catcaatttc ctgttccctg tctacagtca
      781 tgtttttaac ttctgcaaac tgacggactt tagtgttttt cccttttatc ggggaaagtt
      841 gctgattggc tattttattt gtagctactt cagtgaaaag tttatgttcc gggatatatt
      901 tcttcaaaag ggatttaaac ttctcgactg ttttctgttc tccaccttca gcaaagttat
      961 aacttccgtg agtttcacag aaatccagaa gatcaattac atcagaatga tcagcaggaa
     1021 gaagtttatc accgagttcc ttttcacaga aagacttata ttctgctaat tttttttcag
     1081 tggcaatttt ctttagttcg gcctctgcaa attccctggc ctttttttcc tcttctgcct
     1141 tctgctcaag gaaagttatt cttgaagcct tctgttcaag ttctgctatt tcttcttcag
     1201 agtattcaga aaaggctttt ccttttttag atttacaacc ggagttctct ttcttctttc
     1261 cattggaaac ttcttcagtg gtttcttcct ccggggtttc agtggtttcc tcttccgggg
     1321 tttctccctc cactttcttt tttttcatac ctttaacctc cttatagtcc ttttcaggac
     1381 ttatatcttt ttcggcttct tcagccggtt taatttcttc attgaaataa taatcattac
     1441 cccgtaactt atcgcaactc ttatctattg cctcacagca acttttaatg atattgctat
     1501 ccgggtaatc gttacacagg gaacgaattg ccttactgct atccctgatt atgtcacagc
     1561 atttagcaat ccccatacta cccatcaggt cacaatactg agtaatagtt gcacagcagg
     1621 tttctatagt agaaatatcc tctttgctca ttcttgaatt attacaggta ttatttattg
     1681 tatcgcaata ttttttaata tctgcacaat gagtttcagt atgacttaat gtttctgcga
     1741 aattagcgac attaccatga aggggatatt tattcttatg atcgttcatc cattgtttag
     1801 ctttatccat tgtccatccc ttttccttta caaaagttac agcgtgagag gtttctgtat
     1861 catctcccgg taatttaccc aggtgaatta aaataccagg atcgctttta agttccacca
     1921 tacgggtcat tatatcgaac atatcaggag aacggatttt atgtcttatc tctccggggg
     1981 tttcggaaag attatcagta ttttcaggcc ataagtcttt attttctttt actaccttat
     2041 caggtttatc tatctttaag tcattcacta tagatatagt aggattaaga ttatccattc
     2101 tactgaaatt atccgggggt ttttcagata tggtttttgc tatatcatta gactgtgaaa
     2161 aagtcacata ttcttcctcc ttctctttga attttatatc tgccaatcct tttactgccg
     2221 gaggttgagc acctaaaaaa ccaacgtgcc ttaacctacc atctgggtaa aaggaaatag
     2281 atctcttttt ccagaaaccc gagtgaatag cgtttgaaat ttcaggagga acttgcttga
     2341 attttgcaag aagtttatta ccggttcttt ttattgcctc aacccatcca taagcaggtg
     2401 aattatgttc aggaggatgc cccaaacaga gaggggcttc atggtgagta ggattataac
     2461 ttttgactgt ataatcaagt ttatcttcac taaatacatc agttctacct tcagagtccg
     2521 tctgaactcc accttcaaaa acttcaatcc acttaccgtt aagatcaggg ttttctttct
     2581 tctctattct attttccaac tctacgccat tatgatctgt tattgaacca agcattttaa
     2641 ttactcctaa aatcaatatc ttttcttcat aataactaaa atgaataaat aaaactaacg
     2701 aaattgttca ctgaaattat ttcgttagtt tttttctatt aaaaaacgta tatttgaaac
     2761 aggtaggtga ttagtaaatg gcaaaattat ggataaatga acaaaattat attaatatag
     2821 ataaaaaaca acccttgttc tcacaggaaa tagcaaccag gaaaagaagt ataaatttta
     2881 tgtccttcct gggttattta ccaaatcctg atgcagtttt aaaaagaacc gggcagaata
     2941 ttaaagtcta cgaagaactc agaacagaac ctcagacaag aacctgtatt caatccagga
     3001 aatcgggaac aatgagttta cttcatggaa ttaacagggg aaaggcaaaa acggaacata
     3061 gtcagtttat agaggaaaac ttcagtcaat taccaataca gacaataata gaacaaattt
     3121 tagatgcccc agcatatgga ttccaaccct tggaagtaac ctatggaact ttaccatctg
     3181 gtaaaatgga ggctaaaaaa gtagaagcta aacctgtgga atggttcgtt ttcgatgatg
     3241 aaaataatct tcgatttcgt tctaagtggg attatatgat aggtgaatta cttcctgaca
     3301 agaagtttct ttgtccctca tatgaagcca cttataataa cccatacgga aaccctcttc
     3361 tttcttcctg tttctggccc attacattta aaaaaggtgg acttcaattc tgggttgtat
     3421 ttgtcgaaaa atacggtatg cctcacgtaa ttggtaaagt tccccctgga acaaaacaag
     3481 atgacagagg ccgggttatg gaaatgcttg acgctatggt tcaagatgct gtagcagtta
     3541 taaataacga tgaaactata gagttcgttt ctgataccgg gaaatctgca agtagtaata
     3601 cctttagtca attaattaat tactgtaatg atgatatatc aaaggttata cttggtcaga
     3661 cacttacaac tcaagtcggt aatgtaggta gttacgccgc aggtaaagtc catgctacca
     3721 taaggcagga tatagttgac actgatgtta aattagtgaa acagacattt aatcaattaa
     3781 tccggtggat tacagaaatt aactggggaa aagttaacga ttatcctaca tttgatatgt
     3841 gggctgaaga agatatagat cttgccatag ctcagagaga taaaaccctt gtagatgcag
     3901 gactgaagtt tagtaaaaaa tattggcaaa aaacctatgg ttttgaagct gacgattttg
     3961 agttaatacc ttcctctcct ccttccctta ctccttcctc tcctcctata caattcacag
     4021 aagaaggaaa cgagttaaaa gaagataata aactctcttc tgagttttct gaaagtcagg
     4081 atatagaaga tagatttatt caagatgcct ctcttcacct gataggggaa aaatttgttg
     4141 atggaatagt gaagtatata aaatcttgta agagtttcga tgaagccaaa aagggccttt
     4201 ataaacaata tgaaaatata gaccaggggt tatttcaaga ggtaatggaa aaagcacttt
     4261 tctatagtga tattgccggg agaacttcag atattacaaa gtaggtataa aacaatgttt
     4321 actaaaaaag atataaatag gatatataaa atagcttctt ataagaggaa acaagccctg
     4381 aagaattacg ctgaaaaagc agaacaatta accctgaact tcggagattt aaaaatgctt
     4441 tttggacttc ccccgaaaga agccattgaa tattttaaga agaaaaaacc cgagttaacg
     4501 gaaaaatatt atgacctctg gaaagatgcc catgataaat ccttcacaat agccggtatt
     4561 gcttccctta gtttattaac agatattcaa ggaaaacttc aacaggccct taaagaaggt
     4621 atgacctata aacagtttga ggaagaattt gaaagttctg ttttaaaaaa aatgggatgg
     4681 acttcagacg atgaagatat accgccttat agaatagaaa atatcttcag aacaaatata
     4741 ggaggggctt attgtgccgg tagatggcag agcatagaag ataacaaaga gagtagacct
     4801 tatcttatgt atcacacaat ggaagatcca caggtcagaa cacctcacgc cgatatggat
     4861 aaaaaggttt tcagagtaga tgatcccata tgggacactc acgccgctaa aaatggttat
     4921 ggttgccggt gttggatgca agcattatct gaaagagata taaagagaaa aggaattaaa
     4981 gtagaggatt cagggggaag aattattgaa aaggaaattg ttttgaataa ggcagaggat
     5041 ataagagtta aaatctccgt atatattgac ccaaaaggaa accatattcc agttgacccg
     5101 ggcttcgatt ataaccccgg aaagaaaaac tatattccag atcctaaaaa tttccctcct
     5161 gctttaagac caatagcgga gaaacttata gagaatatcc cggaagttaa agagaatata
     5221 aactcagtat gggaaaacgg agttcttata cctgagagaa taagtaaatg gcaagaatca
     5281 aatgaagatg aaagaaaaat attacttaat gaggtattaa gtaagaaatt acaggaagat
     5341 ttaaaaaccc cgttagattt acctaatggt tatatatatg gagaaaatac aaaagaattt
     5401 gataaatatt atggcagaga aagtaatggt tatgttccag cgtattatag taattcagaa
     5461 ggtcgtatag tgtttaattt aagtgttatg gaagataact atagatacat agttcctttg
     5521 catgaaaata ctcatgtttt taataaaaaa cagtttaata gaatgggaga tactttttac
     5581 tctgaaggtt ctactgattt atttgccatg agaaaatatg tagaatatac tggaaaagaa
     5641 gtttatatta gtgattatgg taatatgtgt tctgatagta tgatacagtt aattaaagaa
     5701 tttaaaggta ataaatccga gattttaaat tttttaaaaa atgttattct tggaaaaaag
     5761 gaagatattg aaaaatatag agaagtagta ttaaaaacaa aagatattta ccataattat
     5821 atgacacagc aagaatataa tgaaatgcta aaagatttaa taaaaacaga ggaaatagac
     5881 cctgttataa aaaaagcaat aaagacctat ttaaaggatt ttggaaaaaa agaaatgtta
     5941 aagaaaatat taacctcaga acaaattgac aaagaggtta attatgcaaa agaaaaatta
     6001 aactattctc aggaagaaca ggatgcactg agaagatgtt atgaagatca gaatagaaac
     6061 ttaaaaaatt ataataaagg tgatattaat catagtgatg atttgagaaa agaggtctta
     6121 gagtggtgga caaagataga ttaaatatat taattaatgc tttctattca gagaagaagg
     6181 atattaagaa gttagaaatg ttgaggaatt taatattgaa aatagataat tcttattcta
     6241 tggttttaag tagagatata gaaaacttca aaggacttcc tgaaggagta gtaatctaat
     6301 ggttgaaata accgttgaag atagagaatt acaatctgag ttaaattccc ttaaagagag
     6361 ataccaagat ctctcccctc ttatgcttac cctttcagaa gatttagtat tttttataac
     6421 agaaaacttt gaaactgaag gtaaaagact tcccccggat aaacaatggc ctgaactttc
     6481 agattggaca attaacgaaa gggaatataa aggatattgg cccggaaaaa aacttcatca
     6541 aactggaata ttggttaact caattcactc agaatattcc tcttctgaag cagtagcagg
     6601 gacaaacgtt ccttatgcag agattaatca tttcggagga ataaatgatg gcggtttcta
     6661 cgttccccct cgccccttta tggaactcat tgaccaagac cttgaggata tgatggaaga
     6721 tgtaatgagt tatcttggga aataaaaaaa ccctgtaaaa cacagggttt ttctcagaaa
     6781 gtaaagattt tcttttatct gctgactaat tctgatttga ttgtatttat taatttattc
     6841 cttcttttta tgggtaaaaa ttaagtaaat aatttttaat tacttttctt gtatctatat
     6901 aattaaggta attacattta tcgcaatatc ttggtgtatt taataaatgt gttcccggta
     6961 atattttatc tgatgtatga atatatatat cgaaaacctc accgcattta tcacatttat
     7021 atttatgagc aaaagaagcc atcttaaatc tcctttataa tctcaatatc tctttcctct
     7081 actttatctc cttttttatg gataactata tcggtttctt tcaaatcaac cattttttcc
     7141 tttatcatat atcttattcc ttcttcctct gagtcaaact taatgcaagg tctacttata
     7201 agtttccatt cattaatgat tatatcataa caatacttta aatctttttc tcttttttgt
     7261 aaaatctttg cttcttcctc tgtaaatccc attctatatc tattatttat ttctcttaat
     7321 tcttcttgta aacgagaaac atattctcgc tttgttggga atgtatctaa aaactcttcc
     7381 cacgtaggac tatacattac ttccatagtt ttcctttctc caccgcgtag gtataatggt
     7441 tctattattg atttaaacat ttccctttca atctttttaa ctagatcata aaaatttatt
     7501 gttgggtttt ttcgagaagc cagttctata accggtttat cttttttaag agtaatatta
     7561 attgctaaac tttttgatgg tggaattgac atattttata ttttccttca caatatttac
     7621 aataataatg cttatttttt aactcatatg catcagcacc acatgaagaa cattctttaa
     7681 ccggaggatc tggaatctcc tgttctctta tctctaattg ttttcgatat tcctggaata
     7741 aattactata gttaatttca tagtttgata agtttataac tgctaaaaca ttcccctctg
     7801 aggtttctat aacttcactc ttcatattct ctgttttata tcgtataatc tctttcactc
     7861 cacattcaaa agcacattga gcaatattat atgttctctc ctctattact ggtctaaaaa
     7921 tattattctc tgaaaccaca cattttacat aattctcata gttcatttca ttttgagtca
     7981 atcttaagtt tagttgtttc gttttattta tgggtatatt ctgcagtttc tcaggatatt
     8041 ctaaccttgc ttcacttaag gccagtttta tttcaatctc agcctctttt tttgaaatcc
     8101 tcataacttt tgagataata tcagcaattt cattttccca ctgtctttga ggattttta
//
LOCUS       DAOQWT010000009         8092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_9373_length_8092_cov_2.969516, whole genome shotgun sequence.
ACCESSION   DAOQWT010000009 DAOQWT010000000
VERSION     DAOQWT010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9373_length_8092_cov_2.969516"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKJ95_00505"
     CDS             <1..339
                     /locus_tag="PKJ95_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNR64753.1"
                     /translation="YPIRVKNSLPRINLAKIGQLTFKKLDSKKFPSIDLCYEAIQQGG
                     TFPVVLNAANEVAVSAFLHQGLKFTDIVKVVSMVMKAHQNKLNPTIHDILYQDRESRK
                     LAEKFCKECL"
     gene            341..1405
                     /gene="rseP"
                     /locus_tag="PKJ95_00510"
     CDS             341..1405
                     /gene="rseP"
                     /locus_tag="PKJ95_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNR64754.1"
                     /translation="MITIFSFIFVLGIAILFHEFGHFIIAKISGIKVFKFSLGFGPRI
                     AGISWKGTEYVLCLFPFGGYVKMAGERSQNETEDAQGEREIEESIPESQRFDKKSFLI
                     RTSVIINGPLMNVVLAILLLTLVFFFSGIATVSNSIAEVTPGTPAAFAGLQKGDQVIA
                     INSIKIKNAEDISKIINNNKDNPVSLEILRDKDIFDISLSPEYNEQYDRAMIGITLDI
                     KIKKLSIIDSVKRSIFTTGEIFILIAKGFSEMFTGKIPVELAGPLGIAQMAGEAAQFG
                     FLNLLFFTAVINIFLGLVNLFPIPILDGGQILILAIEKIKGKPLRPEHINFLYIIGIA
                     FVVTIFVIATYQDILRLFTK"
     gene            1423..2505
                     /gene="ispG"
                     /locus_tag="PKJ95_00515"
                     /gene_synonym="gcpE"
     CDS             1423..2505
                     /gene="ispG"
                     /locus_tag="PKJ95_00515"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNR64755.1"
                     /translation="MSNSAIIKRRKSKAAKIGNLFIGGNHPISVQSMTSCDVKDIKST
                     LEQIGKLEAAGCELIRIAFPDIQSCQFIPIIKSKAKTPIMADVHFNYLIALEALKYNI
                     DGIRLNPGNIKKETQIIQVIEKAQKKKAMIRFGINSGSLNKSVMEKYKEPNYLALVEE
                     TEQLLDFLKKLEYQNIVLSIKSSNVLDTIKANEIIAQKCDYPLHIGITEAGFEQSGIV
                     KTTAGISILLYEGIGDTIRVSLTGDPVKEVLVGNDILRTLGIRKKGINLITCPTCGRC
                     QVDIYKIAKKIESNLRGIKTPLTVAIMGCIVNGPGEAKFADAGIAFSKKEAMLFKKGT
                     LIGRYNSKEAIEILESEIRSISKHAD"
     gene            2587..3150
                     /locus_tag="PKJ95_00520"
     CDS             2587..3150
                     /locus_tag="PKJ95_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR64756.1"
                     /translation="MKISVIIPAYNEEATIAEVIKTVKQVPIINSIIVVSDGSTDRTV
                     EIANSLKVNVFEMKKNIGKGGAIKKGFEKTDSEILLFLDADLIGLNTQHVYSLVDPII
                     KDLSDMTVGIFSKGRLLTDLSQMIMPFLSGQRAMKREIIDLIHDLDLLKYGLEMAINK
                     VVKKHDYRVTKVKLTNLTQLLKKKNMG"
     gene            3433..3744
                     /locus_tag="PKJ95_00525"
     CDS             3433..3744
                     /locus_tag="PKJ95_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64757.1"
                     /translation="MLKKIVEELTDIENEAVKIMENAHQEAQNLIEAEKNKQERIKEE
                     LISEFNKAGQEMMNERINNALKKVKEIYNIIEAEKRIIEKDAKDKFDQAVEMVLEQIV
                     K"
     gene            3747..5732
                     /locus_tag="PKJ95_00530"
     CDS             3747..5732
                     /locus_tag="PKJ95_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HNR64758.1"
                     /translation="MAISKISKFHIFAHLSIKESLLSELQRLGCMEITHIEEKVEFHN
                     WKNIEEDIDYNISVKLNNVKFCIDLLSNYDGKKRSGLNSLFASKKIYSYTELIKIAQQ
                     SDYENLYLQCKSFDNELNHLKLEENKLITAEQEIQKWQDLELNFNEIEQRKYIKYTLG
                     SVSKNYFNDLLVDLKKQAKTTIIQTIKEEKNKLYLIVITLKENLPEIEPILQGHHFEI
                     YKYSYSFLGTPKQILKTISKQLDMIAKRRSEIERILTKLRLDNQLIYPLYDILYINQE
                     KEDVKKYLKKSEKIIVLKGWVQNKDIPKLRKRLEKNFTAYEIYFTKPKEDEQTPVSLN
                     NQTLVEPFEVITELYSLPDCHEIDPTPILSIFYFIFFGLCLSDVGYGASLALISYFAI
                     NKLKLEKGAIKFFKLLFYCGIAGALGGVLVGSWFGDILNYLPPQLSNLKNFLIQKLPL
                     FEPTENPLPLLILSLILGIIHIYTGIILKFLDNVRNGKLYEGLMDQISWLLLLTGLIL
                     VLLKGIMPLSIEKFAWTIIIIGILSIILTQGRTKKNILLKIGSGILALYGITGYLSDV
                     LSYSRLFALGLATGIIAQMFNMLATMVNIPYIGFLFTILLLTIGHTFNLLISVLGAFI
                     HDARLQYVEFFSKFYQAGGVPFKPFTLKTRYIKLEDS"
     gene            5812..6285
                     /locus_tag="PKJ95_00535"
     CDS             5812..6285
                     /locus_tag="PKJ95_00535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNR64759.1"
                     /translation="MTDGIILAYLGAALAIGLAGSGSAIGVGIAGTSGAGIMTEDPSK
                     FGLVLILQAIPGTQGIYGLLAGFLVLTRIGIFAGTPAVLTIKQGLLILNACLPIGIVG
                     FFSAIYQGKASAGAIGLIARRPEEAGKAVVIPAMVETYAVLSLLSSILLLNGIVL"
     gene            6307..6915
                     /locus_tag="PKJ95_00540"
     CDS             6307..6915
                     /locus_tag="PKJ95_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HNR64760.1"
                     /translation="MSINKIKEKILGDALEEKGKIQKEAEIEIENIKSLAKKETESIK
                     KEILEKYNQEADLKEKKIITEAILNTKKDILSEKQAIINNVFSEALNRINKLEDEKYL
                     SFMEKLILENIETGDETVYIGKQERKAINHEFIKTINEKLNLQGKKSLLELSKKRLPI
                     MGGVVLGSEKVKKNASLEVILEKVKDEIETKLNQFLFVKHED"
     gene            6917..7945
                     /locus_tag="PKJ95_00545"
     CDS             6917..7945
                     /locus_tag="PKJ95_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNR64761.1"
                     /translation="MLKYINETTNTQEEYGYACARIRELEKFLINKDIIKKMIETPDL
                     ENALKVLVENNYNEYSFENTDPHSVNQVLDNILQRTINIINEISPNPELFHLFKWKYD
                     FLNLKVLLKVKMLDKKAPYPLYDFGNIPLKGLQSAVFEGKYQLIPIKIERIIKQTEAE
                     YLKSENLQLMEIVLDQGYYEIIFDQLEKIDQAFLYYFYKTELDLLNFSIACRCKIRNI
                     KKSELKNILIQFGHLPINKIINFYDNSLSSWPNNFQKTEYYSIFEGGIKDWLQENSLL
                     KLEQLSDDFMLNLLKIGKYTTFGPESIIAYFYAKENDLKNIRIIINGKRYDFSDKIIQ
                     KYIRDTYV"
BASE COUNT         3021 a         1127 c         1413 g         2531 t
ORIGIN      
        1 taccctatta gggtaaaaaa ctctctaccc agaatcaatc tggcaaaaat tggtcaatta
       61 acttttaaaa aacttgattc aaaaaaattc cccagcattg atctgtgcta tgaagcaatt
      121 cagcaagggg gtacttttcc cgtggtatta aatgctgcta atgaagtagc cgtgagtgct
      181 tttttacatc agggattgaa atttacagat attgttaaag ttgtttcaat ggtaatgaaa
      241 gcacatcaga ataaactcaa tcctactatt catgatattc tctatcaaga ccgagaatct
      301 agaaaattag cagaaaagtt ctgcaaggag tgtctctaaa gtgattacta ttttttcatt
      361 tatttttgta ttaggtatag ctattttatt tcatgaattc ggtcatttca tcatagcaaa
      421 aatatctggt ataaaggtgt ttaaattttc tttaggattt ggtcccagaa ttgctggtat
      481 atcgtggaaa ggtactgaat atgtattgtg cttatttcct tttggcggct atgttaaaat
      541 ggctggagaa agatcgcaaa atgaaacaga agatgcccag ggggagagag aaattgagga
      601 gagtattcct gaaagtcaaa gatttgataa aaaatctttt ctaatcagaa catccgttat
      661 tataaatggc cctttaatga atgtggtttt agctatatta ttattaacac tggttttttt
      721 cttttctggt atagccactg tatcaaattc cattgctgag gttactcccg gcacacccgc
      781 tgcttttgca ggactccaaa aaggggacca ggttattgcc attaattcaa tcaaaattaa
      841 aaacgcagag gatatttcta aaataataaa taataacaaa gataatcctg tatctttaga
      901 aatattacga gataaggaca tttttgatat ttcacttagc cccgaataca atgaacaata
      961 cgatcgggca atgataggga taaccctgga tattaaaatt aagaaattat ctattataga
     1021 ttctgtaaaa aggtctattt tcaccactgg cgagatattc attctcattg caaaaggttt
     1081 ctcagaaatg tttaccggta agataccggt agaattagct ggcccattgg gaattgctca
     1141 aatggcagga gaggcagctc aatttggttt cttaaatcta ttatttttca cagcagttat
     1201 taatatcttt ttaggattag ttaacctttt tcctattcct attctggatg gcggccaaat
     1261 tctcatattg gcaattgaaa aaataaaagg caagccatta aggccagaac atattaattt
     1321 cctgtatata atcggtattg cttttgtggt aaccatcttt gttattgcta cctatcagga
     1381 tatattgaga ttattcacca agtaaaggaa tgtgttggaa aagtgtcgaa ttcagctata
     1441 attaaaagaa gaaaaagtaa agcagcaaag atagggaatc tatttattgg cggtaatcac
     1501 ccaatttcag ttcaatcaat gaccagttgt gatgttaaag atattaagtc tacactggaa
     1561 cagattggta aattagaagc tgctggatgt gaacttatca ggattgcttt ccccgatatt
     1621 caatcatgtc agtttattcc aataattaaa agtaaggcaa aaacaccaat tatggcagat
     1681 gtacatttta attatttaat agctttagag gctcttaaat ataatattga tggtatcagg
     1741 ctcaaccctg gaaatataaa aaaagaaacc caaattattc aggttattga aaaagcacaa
     1801 aagaaaaaag cgatgattcg ttttggaatt aattctggtt ctttgaataa aagtgttatg
     1861 gaaaagtata aagaaccaaa ctatttagct ttagttgaag agactgaaca attattagac
     1921 ttcttaaaaa aattagaata tcaaaatatt gttctttcca ttaaatcgag caatgtttta
     1981 gacaccataa aagctaatga gattattgct caaaaatgtg actatcctct gcatattggc
     2041 attaccgaag ctggatttga acaatcagga atagtgaaaa ctacagcagg gatcagtatc
     2101 ttattatatg aagggattgg agatactatt agagtatctt taacaggaga cccggtaaaa
     2161 gaggttctgg tcggaaatga tatattgcgg accctgggta taagaaaaaa aggtatcaac
     2221 ttaataacct gcccaacttg tggaagatgt caggttgaca tctataaaat tgccaagaaa
     2281 atagaatcta atctgcgtgg aattaaaacc cctttaactg ttgccattat gggatgtatt
     2341 gttaatgggc ctggtgaagc aaaatttgcc gatgcaggaa tagcttttag caaaaaagaa
     2401 gcaatgctat tcaagaaagg taccttaatt ggacggtata atagcaaaga ggcaattgaa
     2461 atattagaga gcgagataag gtctatatca aagcatgcag attaatttat gtaatatatc
     2521 taaatattta agttctatta tattaaactt tgtgcagagg aacaatatta aagaggagaa
     2581 ttattgatga aaatttctgt tattatacct gcttacaatg aagaggctac tattgctgaa
     2641 gtaataaaaa cagttaaaca agtaccgatt ataaattcta taatagtagt tagtgatggt
     2701 tccacagacc gaaccgtgga aatagctaat tccttaaagg taaacgtctt cgaaatgaag
     2761 aaaaatatcg gtaaaggtgg agctattaag aagggctttg aaaaaactga ttccgaaata
     2821 ttactctttt tagatgctga tttaattggc ctaaatacac aacatgtcta ctcattggtt
     2881 gatccaatta taaaagattt aagtgatatg acagttggta tttttagtaa agggagatta
     2941 ctaacagact tatctcaaat gataatgccc tttctttcag gtcaaagagc catgaagcgg
     3001 gagataatag accttattca tgacctggat ctgttaaaat atggattaga aatggccatt
     3061 aataaagttg ttaaaaaaca tgattataga gtaactaaag taaagctaac caacctaact
     3121 caacttctaa agaagaaaaa tatgggatag tagagggagc caagcaaagg ttacaaatgt
     3181 atttagacat cgtgaaacaa ctcagaactt gagtaaccta ttataataag caaacagatt
     3241 ttagatccct cccaataagt aatttgactt aaaaaatagt atatgataaa tttcttatta
     3301 tctgctcttt accataaagt ctttaccata aagaaattta ctatgttata ataacagata
     3361 aagagttttt ctatatttta atattttgtt tattttataa aaaagaagaa tttactttct
     3421 ggagtaaagg atatgttgaa aaagatagtg gaagaactaa ctgatataga aaatgaagct
     3481 gtaaaaataa tggaaaatgc ccaccaggaa gctcagaatt taattgaagc agaaaagaat
     3541 aaacaagaaa ggattaaaga agaattaatt tctgagttta acaaagctgg ccaggaaatg
     3601 atgaatgaaa gaatcaataa tgccttaaaa aaggttaagg aaatttataa tattattgaa
     3661 gcagaaaaaa gaataataga aaaagatgca aaggataaat ttgatcaggc tgtagaaatg
     3721 gtcttagagc aaatagtgaa atagacatgg ctatctctaa aataagtaaa ttccatattt
     3781 ttgctcattt atcaataaaa gaatctttgc ttagtgaatt acagagattg ggttgtatgg
     3841 agataaccca tattgaagaa aaagtggaat ttcacaactg gaaaaatatt gaagaagata
     3901 ttgattacaa tatttctgtc aagttaaaca atgttaaatt ttgcattgac ttactttcga
     3961 attatgatgg taaaaaaaga agtggattaa actcattatt tgcttcaaaa aagatttact
     4021 cttatactga attgataaaa atagcacaac agtctgatta tgagaatttg tacttacaat
     4081 gcaaatcttt tgacaatgag cttaaccacc ttaaattaga agagaataaa ctgattactg
     4141 ctgaacaaga gatacaaaaa tggcaggatc tggaactcaa ttttaatgaa attgaacaac
     4201 gaaaatatat taaatataca ctgggttctg tttcaaaaaa ctattttaat gatttattgg
     4261 ttgatttaaa aaagcaagcc aagacaacca tcattcaaac tataaaagaa gaaaagaata
     4321 aattatacct cattgttatt actttaaaag aaaatttacc tgaaattgaa ccaattctac
     4381 aaggacatca ttttgaaata tataaataca gctattcctt tctgggcact cccaaacaaa
     4441 ttctcaaaac tatttcaaaa cagctggata tgattgctaa aagaagatct gaaattgaac
     4501 gaatattgac taaattacgt ctggataatc aacttatcta ccccttatac gatatccttt
     4561 acatcaacca ggaaaaagaa gatgtaaaaa aatatttaaa gaagagtgaa aaaattattg
     4621 ttcttaaggg atgggtacag aataaagata ttccaaaatt acgaaaaaga ttagaaaaaa
     4681 attttactgc ttatgaaatt tactttacta aaccaaaaga ggatgaacag actcctgtct
     4741 cgcttaataa tcagactctg gttgaaccgt ttgaggtaat aactgaactc tatagcttgc
     4801 cagattgcca tgagatagac ccaaccccaa ttctatccat attttatttt attttctttg
     4861 gactctgcct ttctgatgtg gggtatggag catctctggc tttgataagc tactttgcaa
     4921 taaataagct aaaattggaa aaaggagcta taaaattttt taaattgctc ttttattgtg
     4981 gaatagcagg agcgttagga ggggtactgg taggaagctg gtttggtgat attttgaatt
     5041 acctaccacc acaactaagt aatttaaaga attttttaat tcaaaagctc cctctatttg
     5101 aaccaactga gaacccgctt cctttgttga ttctgagctt aatattagga ataattcata
     5161 tttatacggg gataatctta aaatttttag ataatgtcag gaatggaaaa ttatatgagg
     5221 gtttaatgga tcagatatcc tggctattac tcttaaccgg attaatctta gtattattaa
     5281 aagggattat gcctttgagc atagagaaat ttgcctggac aattattata attggtattc
     5341 tatctattat tttaactcaa ggaagaacta aaaagaatat tttattaaag ataggtagcg
     5401 gtatacttgc tttatatggt attacgggct atttaagtga tgtactatct tattcccgat
     5461 tatttgctct tggattggct accggaatta ttgcccaaat gttcaatatg cttgccacta
     5521 tggttaatat tccctatatc ggattcttgt tcaccatact cctgctaaca ataggacata
     5581 ccttcaatct attaatcagt gtattaggtg cctttatcca tgatgcaaga ttgcaatatg
     5641 ttgaattttt ttctaaattc taccaagccg gaggggttcc ctttaagcct ttcactctta
     5701 aaaccagata tattaagctg gaagactctt aaagaacaga acaataacta ttttttttga
     5761 taaaacaaaa tttaacaaaa tgtaaagtaa aaaaaagggg ggatataatt tatgacagat
     5821 ggaattatcc tagcttactt aggagcagct ttagcaatag gattggccgg ttctggttca
     5881 gccataggtg taggcatagc cggtaccagt ggagcaggca ttatgacaga ggatcccagt
     5941 aaatttgggc ttgtgcttat actccaagca attcctggta cacaaggtat ttatggtttg
     6001 ctggctggtt ttttagtctt aaccagaata ggtatctttg ccggtacacc tgcggtttta
     6061 actattaaac aaggacttct tatactgaat gcctgcctac ctataggtat tgtcggtttc
     6121 ttctctgcaa tttatcaggg taaagcatca gcgggtgcaa ttgggcttat tgctcggcga
     6181 ccggaagaag caggaaaagc agtggttatc ccggcaatgg ttgaaactta tgccgtgcta
     6241 tctttattga gttccatatt attgttaaat ggaattgtcc tttaatatct taggagattt
     6301 ttacttatgt ccataaataa aattaaagaa aagatattag gagatgcttt agaggaaaaa
     6361 ggaaagatac aaaaagaggc agaaatagag attgaaaaca taaaaagctt ggcaaaaaaa
     6421 gaaactgagt cgataaaaaa agaaatttta gaaaaatata atcaggaagc tgacttgaag
     6481 gagaaaaaaa ttataacaga ggctattctg aatacaaaaa aagatattct ttctgaaaaa
     6541 caagcaataa taaataatgt tttctctgag gcattaaata gaataaataa attagaagat
     6601 gaaaaatatt tatcttttat ggaaaaactt attctggaaa atatagaaac cggggatgaa
     6661 acggtatata tagggaagca ggaacgaaaa gcaatcaatc atgaatttat taaaacaatc
     6721 aatgaaaaac taaacctaca aggcaaaaag agcctgttgg agttatcaaa aaaacgtctc
     6781 cctatcatgg gtggagtagt cctgggaagc gaaaaagtta aaaaaaatgc ttctttagaa
     6841 gtgattctag aaaaagtaaa agatgaaata gaaacaaaac tgaaccaatt tctttttgtt
     6901 aagcatgagg attaatatgc ttaaatatat caatgaaacc actaatacgc aggaagaata
     6961 cggttatgcc tgtgcaagaa ttagagaatt agaaaaattt ttaataaaca aagatattat
     7021 taagaaaatg attgaaacac cagatttaga aaatgccctt aaagtattag tggaaaataa
     7081 ttataatgaa tattcattcg aaaacacgga ccctcattct gttaatcaag tattagataa
     7141 tattttacaa agaactatca atataattaa tgaaatttca ccaaatcctg aacttttcca
     7201 cctatttaaa tggaaatatg attttctcaa ccttaaagta cttttaaagg ttaaaatgct
     7261 tgacaaaaaa gcgccttacc cattatatga ttttggaaat atccctttaa aggggttaca
     7321 atctgctgtt tttgaaggaa aataccaatt aatacccata aaaattgaga ggattatcaa
     7381 acaaacagaa gctgaatatt taaaatcaga aaatttacaa ttaatggaaa tagtattgga
     7441 tcagggttat tacgaaataa tcttcgacca attggaaaaa attgaccaag cctttttata
     7501 ctatttttat aaaactgaac tggatttatt gaattttagt attgcttgcc gatgcaaaat
     7561 tagaaatatt aaaaaatctg aattaaaaaa catattaata caatttggac atcttccgat
     7621 taataaaata ataaattttt atgataattc tttatcttcc tggcccaata attttcaaaa
     7681 aacagagtac tattccatat ttgagggagg cattaaagat tggctgcagg agaattcttt
     7741 attgaaactt gaacagctaa gtgatgactt tatgttaaat ttactaaaga ttggtaaata
     7801 tactactttt ggaccggaaa gtattattgc ttacttttat gccaaagaga atgatttaaa
     7861 aaatattcga atcattatta atggcaaaag atacgatttt tcagataaaa ttattcaaaa
     7921 atatatcagg gatacttatg tctaatattg caattattgg agaaaaagag attattatag
     7981 gttttagtct tatcgggttt cagttatttc cagtaactga ttcttttgaa gctaaagatg
     8041 ctttagaaaa atgcactact aatagtaatc tcaggatagt atttatcact ga
//
LOCUS       DAOQWT010000010         7917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_9613_length_7917_cov_5.749300, whole genome shotgun sequence.
ACCESSION   DAOQWT010000010 DAOQWT010000000
VERSION     DAOQWT010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9613_length_7917_cov_5.749300"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..363)
                     /locus_tag="PKJ95_00550"
     CDS             complement(70..363)
                     /locus_tag="PKJ95_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64762.1"
                     /translation="MWIYESRKKGGKYKIKKTWEKKEQRKAESYFCLMKGRTWSCRLR
                     FNDVVVKAYNAESGRIFPEPPKEEDVEQFEERVELVIPIKQWRIQKIGRSLCF"
     gene            complement(454..621)
                     /locus_tag="PKJ95_00555"
     CDS             complement(454..621)
                     /locus_tag="PKJ95_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64763.1"
                     /translation="MVEAPVLKPKTLVQIHTLRKERHYCKSGSIGENPVRIASLSGSL
                     ISNRDMSYWQS"
     gene            complement(796..868)
                     /locus_tag="PKJ95_00560"
     tRNA            complement(796..868)
                     /locus_tag="PKJ95_00560"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            1031..1399
                     /locus_tag="PKJ95_00565"
     CDS             1031..1399
                     /locus_tag="PKJ95_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64764.1"
                     /translation="MPSKRGSSLYFEKDGRTWKYCPQCDQAFPFNLVYYGKNPCQANG
                     LNHSCKRCEARRDREYKKKVKAGYVPPEAKRNCQNRNKRKKETRDALKEEMKDLEKKV
                     RGKKKKPREIGEDDLFYGLM"
     gene            complement(1420..1653)
                     /locus_tag="PKJ95_00570"
     CDS             complement(1420..1653)
                     /locus_tag="PKJ95_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64765.1"
                     /translation="MQNEICPFAHGVYCKEDCKLFNRDTKECKLGEKKSDGQFVSILK
                     SDIKYIEGRVNNLELKIQELEAEGKKKKTTASR"
     gene            1730..3277
                     /locus_tag="PKJ95_00575"
     CDS             1730..3277
                     /locus_tag="PKJ95_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64766.1"
                     /translation="MIGNTWSILHSQLKQKKTKPILTDRDYRMVSTPSSFLELGHYDG
                     RRISLFDFQRAYIDDINTFVAVAKSRQLGHSEYGIAGCAMAKSHLYKPLTSILVSYNL
                     KECKEKIQKALLFWEGIHPSLRSPIDHDNAFEIWLKNGSRLISVFSPRGFSRSDIYLD
                     EMSFYENQTKTYTDSTAVIAWGKGKRQLRIGGTPFGKGGLYYDIINKTDGKYLDFSTH
                     IWFWWDCPLYCHNIPLAREQAHLMSTHDRVMKFGTPVIQQFYRNMFLEDFQQEFEICF
                     NDTELSYFPYDLIVQSMSHFSFEDQMKFPETIEQIAPNIQGVLFGGYDIGRTTDNSEF
                     YVLDMRTIDKKAKRKTILRELYHESIADMGLSDQKAKLCRFIDMYHSNIGCIYIDKTG
                     IGFNIAEDLAILYPDKVKGIYFTHESKTQMISTAKGFMTDDTLELMPDRDLIAHFYSI
                     KRHVTSAGNMVFVAEKNGKHHADKCIGVSLACLAARSAIDIAPGIFVFGGEWDFSPEI
                     DMFTNFN"
     gene            3289..3531
                     /locus_tag="PKJ95_00580"
     CDS             3289..3531
                     /locus_tag="PKJ95_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64767.1"
                     /translation="MNIYTECLKDGIDPDAIELESIGNVKGFIEVADEINKSYQEKAK
                     TIDDFENPVPLEPKENPEDKLWTKHFERKYKNMFGD"
     gene            3773..4219
                     /locus_tag="PKJ95_00585"
     CDS             3773..4219
                     /locus_tag="PKJ95_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64768.1"
                     /translation="MALHKINNVEKTPRCKVCGELAGWYDKAVHNVQTKPDKDSMENI
                     GTGKGLVYATCDNPTCPLKLKGEPIVCQHIDHRPDQHKVITMETRKIRSRYDLPYPYK
                     GMDSCMVEGFYTRHDGKLITFQCCRNEACMQHFYEQGKKIGQAPLG"
     gene            4236..5777
                     /locus_tag="PKJ95_00590"
     CDS             4236..5777
                     /locus_tag="PKJ95_00590"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNR64769.1"
                     /translation="MPVATVDKKGKRRPTKVIVKELQEEKEQLQDCDVDPFSFLEKSF
                     PESSYLGNENTLSIPNFLIRGSNGLRKPFLYQYLFDKITWIGAGISKIGDSAFSSGCK
                     IVNAKKGVPIDKKAGDYPAVTFLKELFANPNNDQTFLELYKQVIIDLCVNGKGYFIIE
                     FLEDTPPFRTPIAIYRADFRCMRPVYLHDYLIYSQNVPYLEAKKYKNKIVMWCQEVIE
                     GLESFTEITGKEQNSKTPWIVGNGTNTKYFFPEQVLEIKLYANGVSPLESLEDSIATE
                     IAAKKYTYSYFKNATKTGMVLTLEKGSAADAKQNKEWLMNEYSKPAGAWKPLFLLGGV
                     KMVASGANTSDVQYLEIRAFNKTECCAAIGVPESMFGDGNEEDTINFENNTVVPKENL
                     VLMKLQKKFEDILPWFKGSFVIQSGRKSKASYHMMKIAQLQAMIGGSVNENRELIKLP
                     PKDGEEYDDACLASNIMPVKMLKKTIESKNNSIGPKVVRDNFTGQRRDENIQGGKSGI
                     TNEQR"
     gene            5783..6478
                     /locus_tag="PKJ95_00595"
     CDS             5783..6478
                     /locus_tag="PKJ95_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdF-like putative serine protease
                     domain-containing protein"
                     /protein_id="HNR64770.1"
                     /translation="MEVPGFIPEKYKQIWTDTFNAIYSKKDEHESFRRSGDIYDDIEV
                     KAYRIACGRVKAEVIREFSFNMLVNTPMPIEYISTWDFKDEGITKAKPEDKQVNFNFK
                     KSNVDIGLGVIYGIASKADVVDQEDEIIELIDLKMAAHEFMSESRNGDIDHDWKNHGD
                     VVESLVMDKAFIDMVKSGEIAEGDWFIGYKPNDQEVAKKAASGEDFNGFSIGGICRRV
                     IERGDDSGSNKKA"
     gene            6582..7154
                     /locus_tag="PKJ95_00600"
     CDS             6582..7154
                     /locus_tag="PKJ95_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64771.1"
                     /translation="MTFQEFMEAAYKKFGYGNTPSADTPATIAKAEVSDQKADPLPEF
                     VTKAEFDTQLKTLGDKIDKLTDVLTKGAEVEKAEKENTGKFIADFAEMYKQDREALKA
                     EIAEEIVKAKRAVSPVQEQPSLEELVKKSLHPDSLFGFSNKTGKVMEGIVNKGITDGK
                     YKVNSQPMPVNSALQAFRDLMNCNMTTGGI"
     gene            7156..>7917
                     /locus_tag="PKJ95_00605"
     CDS             7156..>7917
                     /locus_tag="PKJ95_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64772.1"
                     /translation="MDLLIQMLLGALMEKAQLDSTTAANFHRQDLSSTIYLEGMPEAK
                     FFLRLPTTPAKDKLHYWSKTSISAPDGTSGYVTEGARPSDKHTTPDRLYNNLFRRGLA
                     CKITATEMALYAASGGWQLSEAGRLQAFNDLMAYQMYMNTKYLLSLLNYVFINGVGTT
                     TIDGGSADVQCDGLIQALYYYDSVPTAQGNIVDASGASLTEAMMISLGKLIADRRTGL
                     KPQVQYVTSAQKKIINTWGGTIFFTRNENVPAGRDV"
BASE COUNT         2657 a         1389 c         1589 g         2282 t
ORIGIN      
        1 cttcgatcat gaataagagt atccataacc atccacaaaa aaaacagcat ggcatattaa
       61 taacctcctt taaaaacata aagatcttcc tattttctgt atcctccatt gctttatagg
      121 aataaccagt tctacccttt cttcaaattg ctcaacatct tcttcctttg gaggttccgg
      181 gaagatcctt ccactttcgg cgttgtaagc tttaactacc acatcgttaa accttaacct
      241 acaactccag gttcgacctt tcattagaca gaagtaactc tctgcctttc tttgttcttt
      301 cttctcccat gtctttttaa tcttatattt tcctcctttc tttctacttt cgtagatcca
      361 cataaattat cctccttaaa aaaaataggg gcagatgccc ccaatgtcaa aagaactaac
      421 aaaacataca tcctaccaag cagtcaatcc tgattaagac tgccaatagc tcatatcccg
      481 gttacttatt agagaaccgg aaagactggc tatcctgaca ggattttcac ctatgcttcc
      541 actcttgcaa tagtggcgtt cttttctcag agtgtggatt tgaaccaacg tcttcggctt
      601 taataccggc gcttctacca attaagctaa cctctgataa tctttgatac tacaggataa
      661 ttgacacctg attgtccgtc gaagtgtcat acgggtgttt cgtgtccacg cgacgagttt
      721 caccctgcaa gttatgaggt aaattcctct cgtaaaacct tgtacttcag tatttacgac
      781 cacaggggtt ccctctggca gagaaggcag gagtcgaacc tgcgccttcc tgttactaca
      841 ggatgctctt ccattgagct tcttctctat accccggcac accttattaa tcaggccaag
      901 tccggggccg gttgacttta aatcaaacct gtgattaaat aatatcataa gtataaatct
      961 atgtcaatat ttattacgaa aaatttttaa ttaaatttgg cgtaatatat aattttaaag
     1021 gaatatcgaa atgccatcaa agagaggtag ttctttatat tttgaaaaag acggaaggac
     1081 atggaaatac tgtccgcagt gtgaccaggc atttcctttt aacctggtat attacgggaa
     1141 aaatccatgt caggcaaatg gattaaatca tagttgtaaa cggtgcgagg cccggaggga
     1201 cagagagtat aagaagaaag taaaagcagg gtacgtccca ccggaggcaa aaaggaattg
     1261 ccagaacagg aacaaaagaa agaaagaaac cagagacgca ttaaaagagg aaatgaaaga
     1321 tctggagaag aaagttcggg gtaaaaaaaa gaaacccaga gaaataggtg aagatgatct
     1381 attctatggg ttaatgtaag acaaacaaga aagggaggtt tatcttgaag cagtggtttt
     1441 tttcttctta ccttctgctt caagttcctg tatttttaat tccaggttat tcaccctgcc
     1501 ttctatatat ttaatatcac ttttcaatat agatacaaac tgtccatctg attttttctc
     1561 tcccaattta cattcttttg tatctctatt aaatagctta cagtcttctt tacagtaaac
     1621 cccatgtgca aaggggcata tttcattctg cataataatt ctccttgatt tatatatttt
     1681 gtatatagta taatacaggt atctattgaa atcaagaggt taaaataaat tgataggtaa
     1741 tacctggagt atattacaca gccagttaaa acagaagaaa acaaagccaa tccttaccga
     1801 tagagattat cgtatggtat ctaccccttc ttcttttttg gaactcggtc attatgacgg
     1861 caggagaatt tctctttttg attttcaaag agcgtatata gatgatatta atacttttgt
     1921 tgcagtagca aaatcaagac aattagggca tagcgaatat gggattgcag gttgcgctat
     1981 ggcaaaatct catttatata aaccattaac ttctatattg gtttcttata atttaaaaga
     2041 atgtaaggaa aaaatacaaa aggctttatt gttctgggaa ggtattcacc cgtctttaag
     2101 atctcctatt gatcacgata acgcctttga aatatggctt aaaaatggca gccgtttaat
     2161 ttctgtcttt tcgcctcgtg gattctctcg ttcagatata tatctggatg aaatgtcctt
     2221 ttatgaaaac cagacaaaaa cctatactga cagcactgcc gttatagcct ggggcaaagg
     2281 taaaaggcag ttaaggatag gcggcactcc ctttggtaaa ggcggcctgt attacgatat
     2341 aataaataag acagatggga agtacctgga tttctctacc catatatggt tctggtggga
     2401 ttgtccgtta tattgccata atattccttt agccagggaa caggcacatc taatgtcaac
     2461 ccatgacagg gttatgaagt ttggaacccc tgttattcag cagttctaca gaaatatgtt
     2521 cctggaagat tttcagcagg aatttgagat atgttttaac gatacagagt tatcctattt
     2581 cccttacgac ttaatagttc aatcaatgtc tcatttcagt tttgaagacc agatgaaatt
     2641 ccctgagact atagagcaga ttgctccgaa tattcagggt gttttattcg gagggtacga
     2701 cattggaaga accactgata attcagagtt ctatgtactg gatatgagga ctatagataa
     2761 aaaagcaaaa aggaaaacta ttctccggga actttatcat gaaagcattg cagatatggg
     2821 gctgtctgac cagaaagcaa agttatgtag atttattgat atgtatcatt caaatatcgg
     2881 ttgtatctat attgataaaa ccggcatagg atttaatatt gcagaagatc ttgctatttt
     2941 ataccccgat aaagttaaag gcatctattt tacccatgaa agtaaaactc aaatgatttc
     3001 tactgctaaa ggttttatga ctgatgatac acttgagctt atgcctgaca gagatttaat
     3061 tgcacatttt tattcaatca aaagacatgt tacttctgca gggaatatgg tttttgtcgc
     3121 agaaaagaac ggcaaacacc atgcagataa atgtatcggt gtttcccttg cctgtttagc
     3181 tgccagaagt gcaatcgaca ttgcgcctgg aatattcgtt tttggtggag aatgggattt
     3241 ttctcctgaa atagacatgt ttacaaattt taattgagga ggcttattat gaatatttat
     3301 acagagtgtt taaaagatgg tatagaccct gatgctatag aactagagtc tataggtaat
     3361 gtaaaaggct ttatcgaggt tgccgatgaa ataaataaat cttaccagga aaaagcaaaa
     3421 actatagatg attttgagaa ccctgttcct ctggaaccaa aagaaaaccc ggaggataaa
     3481 ctatggacca aacattttga aaggaaatat aaaaatatgt ttggtgattg acagggaaat
     3541 tatctttatg ttattataaa catcacgcat ctattattta tatttacact attcgttact
     3601 aaaccagaga actcgcaatc ctctggtttt tcattttaaa ataaatttcc attaccctct
     3661 tgactttcta cttcttatac ctgataatat gtgtataatc ttaaactata gattattatg
     3721 aaatacaaca gtatttcata taacataaat gtatagaatg gagttgtata gaatggcttt
     3781 acataaaata aataatgtag agaagacccc acgctgtaag gtatgcggcg aacttgccgg
     3841 atggtatgac aaggcggttc ataatgtcca gacgaaacct gataaagaca gtatggaaaa
     3901 tataggcaca gggaaaggat tggtttatgc aacatgtgat aatcccacct gtccgcttaa
     3961 attaaaaggc gagcctatag tatgccagca tatagatcac aggccggacc aacataaggt
     4021 aataacaatg gaaacccgga agataagaag cagatacgac ctgccatatc cctataaagg
     4081 aatggatagt tgtatggttg aggggttcta tacaagacac gacggtaaat taataacctt
     4141 ccagtgctgc aggaatgaag cctgtatgca acacttttat gaacaaggta aaaaaatagg
     4201 acaggcccca ttaggataac aaggaggata gataattgcc ggttgctact gtagataaaa
     4261 aaggtaaaag aagacctact aaagttatcg taaaggaatt acaggaagag aaggaacagt
     4321 tacaggattg tgatgtagat cctttttctt ttttagagaa gagtttccct gagagttcat
     4381 atctgggaaa tgaaaatacc ctgtcaattc caaacttctt aattcgcggt tccaatggat
     4441 taagaaaacc ttttttatat cagtacctat ttgataaaat tacctggata ggtgcaggta
     4501 tttcaaagat aggcgattcc gctttttcct ccggttgtaa aatagtaaac gctaaaaagg
     4561 gcgttcctat agataaaaaa gcaggtgatt accctgccgt tacattctta aaagaattgt
     4621 tcgccaatcc gaataatgat cagacttttc ttgaattata taaacaggtg attatagatc
     4681 tttgtgtaaa tgggaaaggt tattttatta tagagttcct tgaggatact cctccattta
     4741 gaacccctat tgcaatatat agagcagatt tccgatgtat gcgacctgtc tatcttcatg
     4801 attatttgat atacagtcag aatgtccctt atttagaagc aaaaaaatat aagaataaaa
     4861 tagttatgtg gtgtcaggaa gttatagagg ggttagaatc gtttactgaa attacgggaa
     4921 aagagcaaaa ttcaaaaact ccctggatag tagggaatgg aacgaataca aaatactttt
     4981 tcccggagca ggttttagag ataaagcttt atgcaaacgg tgtttcccct ttagagtctt
     5041 tagaagatag cattgcaact gagattgcag caaaaaaata tacatattcg tattttaaaa
     5101 atgcaacaaa aaccggtatg gtattgaccc ttgaaaaagg ttcagcagca gacgcgaagc
     5161 aaaataaaga atggttaatg aatgaatatt caaaacctgc cggggcatgg aaacctctct
     5221 ttttattggg tggcgttaaa atggttgcct ccggcgcaaa tacatcagat gtgcagtatt
     5281 tagaaatcag ggcatttaat aaaactgaat gttgcgccgc cataggcgta ccggaaagta
     5341 tgtttggaga cggtaacgaa gaggacacaa ttaacttcga aaataataca gtcgttccaa
     5401 aagaaaacct tgtgttgatg aaactgcaga agaaatttga agatatactt ccctggttca
     5461 aaggaagttt tgttatccaa tctggcagaa aatcaaaagc cagttaccat atgatgaaga
     5521 tagctcagtt acaggctatg atcggcggtt ctgtaaatga aaacagagaa ctcattaaac
     5581 tgcctccgaa agacggggaa gaatatgacg atgcttgcct tgcatcaaat ataatgcctg
     5641 taaagatgtt aaagaaaact atagagtcaa aaaataatag tataggccct aaagtggtaa
     5701 gggataactt taccggacag agaagagacg aaaatatcca gggtggtaag tctggaataa
     5761 caaatgaaca gaggtgagag taatggaagt tcccggtttt atacctgaga aatataaaca
     5821 aatatggact gatacattca atgccattta ttctaaaaaa gatgaacatg aatcttttag
     5881 aagaagtggt gatatttacg atgatataga agttaaagcc tatagaatag cgtgtggcag
     5941 agtcaaagct gaagttatca gagagttttc ttttaatatg ctcgttaaca cacctatgcc
     6001 tatagaatat atatcaacct gggattttaa ggatgaaggc ataacaaaag caaaaccaga
     6061 agataagcag gttaatttta actttaaaaa atccaatgtg gatattggac tcggagtaat
     6121 ttatggtatc gcttctaaag ctgatgtagt ggatcaggaa gacgagatca ttgaattaat
     6181 agatttgaag atggcggcac atgagtttat gtctgaaagc agaaatggtg atatagacca
     6241 tgactggaaa aatcatggtg atgtagtgga atccttagtt atggacaaag cttttatcga
     6301 catggttaaa tccggtgaaa ttgcagaagg tgactggttt ataggttata agccaaacga
     6361 tcaggaggtt gcaaaaaaag ccgcatctgg agaagatttc aacgggtttt cgattggcgg
     6421 catatgccgc agggtaatcg aaaggggtga cgatagtggc agtaacaaaa aagcttaaag
     6481 atttaaaaat aaaatctata agtttggtat ccaataaatc ccgaccagtt aatcaatatt
     6541 cgaaattgac tttcgtaaag tcaaagaagg aggaaaagaa tatgacattt caagagttca
     6601 tggaagctgc ttataagaaa ttcggatatg gaaacactcc ttctgcagat acccctgcaa
     6661 ccatagcaaa agcagaggtt tcagatcaga aagcagaccc attacccgaa tttgtaacaa
     6721 aagcagaatt tgatactcag ttaaaaactc ttggcgataa gatagataaa cttaccgatg
     6781 ttttaaccaa aggcgcagaa gttgaaaaag ccgaaaaaga aaacaccgga aagtttattg
     6841 ccgatttcgc ggagatgtat aaacaggaca gagaagctct taaagctgaa attgcagaag
     6901 agattgtaaa agctaaaaga gccgtatccc ctgtacagga acagccctct cttgaggaat
     6961 tagtgaagaa atcccttcac ccggatagtt tattcggttt cagtaataaa accggtaagg
     7021 ttatggaagg catagtaaat aaagggatta ctgacggtaa atataaagta aactcacagc
     7081 ctatgcctgt caattcagca cttcaggcat tccgtgacct gatgaactgc aatatgacga
     7141 caggaggtat ttaatatgga tttacttatt caaatgttat tgggtgcttt aatggaaaaa
     7201 gctcagttag attccactac agcagctaat tttcaccgcc aggatttaag ttctactata
     7261 tacctcgaag gtatgcctga agcgaagttt ttcttgagac ttcctactac accagcaaaa
     7321 gataagttgc attactggag taagacttcc atatctgctc ctgacggcac ttccgggtat
     7381 gttacagaag gtgcaagacc ttcagataaa cacaccacac ctgacagatt gtataacaat
     7441 ctctttaggc gcggtctggc ctgcaaaatt actgctacag agatggctct ttatgccgcc
     7501 tccggtggat ggcagttatc cgaagcagga agattacagg cgttcaacga tctgatggcc
     7561 tatcagatgt atatgaatac aaagtattta ctcagtttac tgaactatgt gttcataaac
     7621 ggtgtaggta ctacgactat agacggcggt tctgctgatg tacagtgcga cggactcata
     7681 caggcattat actattatga ctctgttcca acggcacagg gtaacatagt agatgcctcc
     7741 ggtgcatctc ttacagaagc aatgatgatt tctctcggta aactcattgc agatcgtaga
     7801 accggattaa aaccacaggt gcagtatgtt acttccgcac agaagaagat aataaatacc
     7861 tggggcggta ctatcttctt tacaagaaat gagaacgtac ctgccggaag agatgtg
//
LOCUS       DAOQWT010000011         7838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_9717_length_7838_cov_3.237184, whole genome shotgun sequence.
ACCESSION   DAOQWT010000011 DAOQWT010000000
VERSION     DAOQWT010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9717_length_7838_cov_3.237184"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(577..1413)
                     /locus_tag="PKJ95_00610"
     CDS             complement(577..1413)
                     /locus_tag="PKJ95_00610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HNR64773.1"
                     /translation="MEYSVPTLSIVFMVIVALTGIIIPIVLFTIFRKKYKANIAPFFI
                     GCTVFIIFALLLERFFITFLFKMAAGKVIRSSIWLYGIFGGLMAGLFEETGRFTAFKT
                     ILKKSRSNDANALMYGAGHGGFEAFYILVFSMISNIIMSVMLNLGLQDKLISGVTDEA
                     KLRTLYATFAVLAGTPPVNFLMGIVERIAAVALHISLSVLVWFAAGKEARCFWFYPLA
                     ILLHSAVDAIAVIMSKYLPNVWAILAVIYVLSTCCVMLALKVWNIYSSQSDISGISAG
                     TE"
     gene            complement(1505..1957)
                     /locus_tag="PKJ95_00615"
     CDS             complement(1505..1957)
                     /locus_tag="PKJ95_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64774.1"
                     /translation="MEIEKRLIMVQNTYAASIAETVNNYEKLGALEAIVERRKERQVQ
                     TAPYLNQQLDIQSVEDVFYKLYEIYGCANWTVKKIEDGYIAMATSCKLCALTRKMGGA
                     NPCNGWCLDPMIAMLSCVSKIDSKQITVESTLMNDDCCKVSIDDRAER"
     gene            complement(2082..2636)
                     /locus_tag="PKJ95_00620"
     CDS             complement(2082..2636)
                     /locus_tag="PKJ95_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNR64775.1"
                     /translation="MTEKDLRERIKEVAVEHFDTNGYHGTTIRNIAKDVNCSLPMIYY
                     YYKNKKELFDEIIKKEFFNLLKKQASLLKTDNIIDFYTKFIFDLNSLSNYDKKVYRLG
                     IKVYLSFEGDEELMKLMDEWEKSILPRHREILKPHLKNVNNEKAIVRTLVHLLETMIE
                     NIVVKNRYLPEDEIREEISIVLKS"
     gene            2907..3572
                     /locus_tag="PKJ95_00625"
     CDS             2907..3572
                     /locus_tag="PKJ95_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNR64776.1"
                     /translation="MNKKSILKISFLNQDYGKGQEIINSITHGIGAILSIVGLIMLLY
                     ISIEKEDIWRIVSFAIYGTTLVFLYVSSTIYHSVTDKRKKYIFRILDHIAIYLLIAGS
                     YTPLTLVLLRGSLGWIIFGVVWLIALAGIFMKIFFFSKTKILSMILYIIMGWLIVVVT
                     KPIISILPTGILYLLIVGGLCYSLGIIFYITPKIPYNHTIWHIFVLAGSFTHFLGYYF
                     FLA"
     gene            complement(3696..3902)
                     /locus_tag="PKJ95_00630"
     CDS             complement(3696..3902)
                     /locus_tag="PKJ95_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNR64777.1"
                     /translation="MGSVYKIIEIVGTSESSWEDAAKNALDTAAKTIQDIRVAEVNKL
                     DIKISTDGKIFYRARLNVSFKYRT"
     gene            complement(3928..4182)
                     /locus_tag="PKJ95_00635"
     CDS             complement(3928..4182)
                     /locus_tag="PKJ95_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004005886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HNR64778.1"
                     /translation="MNRLPDSELVMIPGGTLITDTPEEGRALAMLIARHTIHNIQPDL
                     DVLKAHREEYSTNPNSLTAAAQVVAIEFQTIAAANNYWRK"
     gene            complement(4249..4674)
                     /locus_tag="PKJ95_00640"
     CDS             complement(4249..4674)
                     /locus_tag="PKJ95_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNR64779.1"
                     /translation="MKDTVDRFNRFAAAGKDEDFGRGEFNYDREWTTFPPTIPGVEWP
                     PKDSPNYTMYPLSEEGPYYAVILAAGTLDTNGGPIINKKAQVLDNFDNPIPGLYGAGN
                     CIASPTANAYWGGGSTIGPALTYGHIAAWNAVTEPVKQI"
     gene            complement(4722..5885)
                     /locus_tag="PKJ95_00645"
     CDS             complement(4722..5885)
                     /locus_tag="PKJ95_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR64780.1"
                     /translation="MDFVQEVDILVVGSGAAAFAAAITAKEKGAEVIMLEKGSIVGGT
                     TMRSGGGFWTPNNRFQKKMGIRDEKKDAINYMARLSYPQLYNPDDPMLGLIKNDFELI
                     DTYYDRAHQAVEFLEDCGAIKTINEINWTGKPQVDYMDHLPENKGVRGRCLYTQNKDG
                     KLSYGGELVRQLKAWANTNNIPIITNHEAKHILRNAQGEVIGLTVLKEGKNTLNFRAR
                     KAVIFGSGGYTHNTEFMLHFQRGPHFGGCSAPTNKGDFIRMGGEIGAKLGNMAGAWRA
                     QLILENAIKYPDGVHNVFYIGGDSVLEVNKYGLRVMDEKRNYSDRAMTHFVWNPQYAE
                     WTNMLLFMIFDQRTATYWQGFTPFPVQGTEVEHIITGGNFDELAENISARLKK"
     gene            complement(5994..6212)
                     /locus_tag="PKJ95_00650"
     CDS             complement(5994..6212)
                     /locus_tag="PKJ95_00650"
                     /inference="COORDINATES: protein motif:HMM:NF022420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2769 domain-containing protein"
                     /protein_id="HNR64781.1"
                     /translation="MANKEVDKELQAQCLCPGCPSYLDCGDPLAYCLVGEVSKCINTR
                     QGCICDSCPVWEKKRFENGYYCITKGGE"
     gene            complement(6829..>7838)
                     /locus_tag="PKJ95_00655"
     CDS             complement(6829..>7838)
                     /locus_tag="PKJ95_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNR64782.1"
                     /translation="GAEHYLKKGELLPDSVLEEIKGMDAIYLGAVGDPQVKPGILEKE
                     ILLKIRFSLDQYINLRPIRLLQGIESPLRNKTFKNINFYVVRENTEDFYIGTGSSFKG
                     KEYHSDHVIKRNLFQSNIQIDFQLDGEEEIGYQLGLLTRKGSDRIIRYAFELAEKKGL
                     SKVSTVDKANVLSDMYGLWRRVFKDVSRDFPDISTDYYYVDAMTMLMVTNPERFQIVV
                     TPNMFGDIITDLGAAIQGGIGIAASANINPEGISMFEPIHGSAPDIKNQGKANPIAAI
                     LAGAMMLDSLGETKIASTIENVTQEVLKEGKIKPIEFGGKASTSEIGDGIIKKLKKLL
                     N"
BASE COUNT         2335 a         1584 c         1235 g         2684 t
ORIGIN      
        1 gttgaaaatg acaaatcgag taagtttatg atcgtaacat ttctatgtat agagatcatg
       61 taaacacttg atacaaaaat aatagtctgt attatagttg tcgttattaa taaacgaaaa
      121 ttcagtttta cattaaaagc ttttttgtaa aaaaatctta cagtactatc aggataaatt
      181 tttttaaaca ttagcagcaa tacataggtg gaggtccatg ctgttatggc aattaaccat
      241 ctcattacca atggagtccc atggaatatt actgttgcta taccacctaa tgtaaaaagc
      301 aataatccaa acaatagata agtatagatg acatattgaa ttatatagtt ttttgatttt
      361 ataacattca ccttcgcgaa acctcttaag gtttaagatg tacctttata aaataaacgc
      421 ttccctctgc tttagaacct ttaacagaaa aagtataagt gccggtttct ttaattccaa
      481 ttatgaattt actggtttcc acatcattac ctcgatatgc gcttatacta tattttaact
      541 acatcaaaaa taagagatat tattttgcca catttcctac tctgttcccg ctgaaattcc
      601 cgaaatatca ctttgagaag aatatatatt ccatactttt aaagcaagca taacgcaaca
      661 ggtagagagc acgtaaatta ctgctaaaat tgcccatacg tttggaagat atttagacat
      721 aattaccgca atcgcatcaa ctgcagaatg taataatatt gcgagaggat agaaccagaa
      781 gcacctggct tcctttcctg cagcaaacca taccaaaact gataaagaaa tatgaagagc
      841 tactgccgca attcgttcta ctattcccat aaggaagttt acaggagggg taccagctaa
      901 tacagcaaat gttgcgtaaa gtgtccgtaa ttttgcttca tcggtgacac cggatattaa
      961 tttgtcctgc aaaccgaggt tcaacatgac cgacattata atattagaaa tcatgctgaa
     1021 aacaagaatg taaaaagctt caaaaccacc atgtcccgct ccgtacatca gcgcatttgc
     1081 gtcattactt cgactctttt ttaatatagt tttaaaagca gtaaagcgac cagtttcctc
     1141 gaagagtcct gccatcagtc caccaaaaat gccatagagc catatgctgc ttctaattac
     1201 ctttccggct gccattttaa aaagaaatgt aataaaaaag cgttcaagta ataatgcgaa
     1261 tatgataaat actgtgcaac caataaagaa aggtgcaata tttgctttat atttttttct
     1321 gaatattgta aaaagaacaa tcggaataat tatgccagtc agtgcaacaa ttaccataaa
     1381 aactattgac aatgtaggta cagagtattc cataagcata ccctcctttt cttttatctc
     1441 tatcctttta accctttata aacgttatac ttatgattat cgctcttatg tattctttct
     1501 ttcgttaacg ttctgctctg tcatcgatag atactttgca gcaatcatcg ttcattaaag
     1561 tactctcaac agttatttgt ttgctatcaa ttttgctcac gcaggataac attgcaatca
     1621 ttgggtcaag gcaccagcca ttacaaggat tcgctccgcc catttttctt gtcagcgcac
     1681 agagcttaca tgaagtcgcc atagcaatgt aaccatcctc tattttcttt acagtccagt
     1741 ttgcgcagcc atagatttca taaagcttat agaatacatc ttcaacactt tgaatatcca
     1801 attgctgatt caaataaggt gctgtttgta cctgcctttc ttttcgtctt tccacaattg
     1861 cctctaacgc ccccagtttt tcatagttgt taacagtctc cgcgatagat gccgcatacg
     1921 tgttttgcac catgatgagt cttttttcga tttccatttt tatctccttt attaatatta
     1981 tttttttaag ctttttttat ccatttgcaa aatataaaac ggtaattttg catatcgata
     2041 cataccgaat tatatttgat taaggttttc gcgaattatc ctcaactttt tagaacaatg
     2101 gatatctctt ccctgatttc atcttctggt agatatctgt ttttcacgac aatgttttcg
     2161 atcatcgttt ctagtaaatg taccaatgtt ctcactattg ctttttcatt attaacattc
     2221 ttcaagtggg gcttgagaat ctcacggtgc ctgggtaata tactcttttc ccactcatcc
     2281 ataagtttca tcaattcctc gtctccttcg aacgataagt aaaccttgat accaaggcga
     2341 tatacttttt tatcgtagtt actcaaggag tttaaatcga aaatgaactt tgtataaaaa
     2401 tcaataatat tgtcagtttt caataatgat gcctgctttt ttaaaagatt aaaaaattct
     2461 tttttaataa tttcatcaaa caactctttt ttatttttgt agtaataata gatcataggc
     2521 agtgagcaat tcacatcttt agcgatattt cgtattgttg ttccatgata accgttagtg
     2581 tcgaaatgct ctactgccac ttcctttatt cgctcccgca aatccttttc tgtcataagc
     2641 ttcctccttt acttaacgtt cgttagtatt gtagcatgcc aaatttagcc tgtcaatatt
     2701 tttggtaaaa aatttgaaga taaaataaaa actaatgaaa gcaatagtta atgccaggag
     2761 gatttggtgg ctatttaatt ttacagaaaa attgggattg acccatattt tgaaaataaa
     2821 tatcactatt aactaataaa ctctctgaat aattgaatgt tagtcaaaat tttgtggtaa
     2881 tataatttta gaggtcagta atcattatga acaagaagag tattttaaaa atatcttttt
     2941 taaatcagga ttatggaaaa ggccaggaaa ttattaatag cattacacat gggattgggg
     3001 caattctaag cattgttggc ttaattatgc tcctttatat atctatagag aaagaggaca
     3061 tctggcgaat agtgagcttt gctatttatg gaacaacact tgtcttcctt tatgttagtt
     3121 caactatata tcatagtgtt actgataaaa gaaaaaaata tatatttcgc attctggatc
     3181 atatcgctat atatctcctc attgccggct cttatacccc attaaccctg gtattattac
     3241 gagggtcatt gggctggata atatttggag tagtatggct tattgcactt gctggtatct
     3301 ttatgaagat attctttttc agtaaaacaa aaatcctttc tatgattcta tatataataa
     3361 tgggttggct tattgtggtg gttactaaac caatcatcag tatacttccc actggaatac
     3421 tatatctatt aattgtgggg ggactctgtt attctctggg gattatcttc tatataactc
     3481 ctaaaatacc ttataatcac acaatctggc atatctttgt tctggcaggt agttttaccc
     3541 atttcctggg ttattatttc tttctggcgt aaaataataa acattattta tcaatcaaga
     3601 ttaaacagag taaacagggt aaaacattaa ttacctgggc tactctgttt aatcttggct
     3661 tttaagataa tcaattatta tggaataatt attaactatg ttctgtactt aaaggataca
     3721 tttaatcttg ctctataaaa gattttacca tcagtgctta tctttatatc gagcttattt
     3781 acctcagcaa ctctgatatc ctgaatagtt tttgccgctg tgtccagcgc atttttggct
     3841 gcatcttccc aggaactttc gctggtacca acaatctcaa ttattttata gacacttccc
     3901 atatctgacc tccttaaaga atatttatta tttacgccag tagttgttgg cagctgctat
     3961 ggtctggaat tcaatagcca ccacctgggc agcagcagta agactatttg ggtttgtaga
     4021 atactcctca cgatgtgctt tcagtacatc aagatcgggc tgtatattgt gaatagtatg
     4081 acgggcaatc agcatagcta aagcacgtcc ctcctccggt gtatcagtaa taagcgtacc
     4141 tcctggtatc attaccaatt ctgaatctgg tagacgattc attttgttcc tccttttctt
     4201 ttttaattaa actctaacaa tcttttttta ttttctttaa aaaataactt aaatttgttt
     4261 aacaggttct gtaactgcat tccaggcagc aatatgtcca taggtcaatg ccggaccaat
     4321 cgtgctgcca cctccccaat aggcattggc agttggagag gcaatacagt ttccggctcc
     4381 gtataaacca ggaattggat tatcaaaatt atccagtacc tgagctttct tattaattat
     4441 aggacctccg ttggtatcca atgttccggc tgccaaaatt acggcataat aaggtccttc
     4501 ttcactcaaa ggatacatag tatagtttgg actatcctta ggcggccatt ccactccagg
     4561 gatggtcggt gggaaagtag tccattcccg gtcatagtta aactcacctc gaccaaaatc
     4621 ttcatccttc ccggctgccg caaagcgatt gaatctgtca acagtatctt tcagattttc
     4681 taaaaatctt ttatctagag aaaaacctcc agtatggggt gctatttttt taaccttgct
     4741 gaaatgtttt ctgccagctc atcaaagttg cctcctgtga taatatgctc aacctctgtc
     4801 ccttgaactg ggaagggtgt gaagccctgc cagtaagtgg cagtacgctg gtcgaaaatc
     4861 ataaacagca gcatattggt ccattcggca tattggggat tccatacaaa atgagtcata
     4921 gctctatcag aatagttacg cttctcatcc attaccctca aaccgtattt gttgacctcc
     4981 aaaacactgt caccaccaat gtagaagaca ttatgaactc catcaggata tttaatggca
     5041 ttttccagta tgagctgcgc tctccaggca ccggccatat tgcccagctt ggcacctatc
     5101 tctccaccca ttctgataaa atctccctta ttggtcggcg ctgaacagcc tccaaaatga
     5161 ggtccacgct ggaagtgcag cataaattca gtgttgtggg tgtagcctcc gctgccaaat
     5221 atcaccgctt ttctggcacg gaaattcaga gtatttttcc cctcttttaa aactgtgagg
     5281 cctattacct cgccttgagc atttcgtaaa atatgttttg cttcatgatt ggtgattata
     5341 gggatattat ttgtatttgc ccatgctttt aattgcctga ccaattctcc tccgtaagag
     5401 agtttgccat ccttattctg ggtatagaga catcttcccc gtaccccttt attttctgga
     5461 aggtggtcca tataatccac ctggggcttc cctgtccagt ttatctcatt tattgtttta
     5521 attgccccac aatcttctaa aaactcaact gcctgatgtg cccggtcata gtaggtatct
     5581 attaattcaa aatcgttctt aataagcccc aacatagggt catcaggatt atacaattgg
     5641 ggataagaaa ggcgagccat gtaatttata gcatccttct tttcatctct tattcccatc
     5701 tttttttgga agcggttatt tggtgtccaa aaacctcctc cggagcgcat agtagttcca
     5761 ccaacaatag aacccttttc cagcataatg acctcggcgc ccttttcttt ggcagttatt
     5821 gcggcagcaa aagctgctgc cccgctgccc accactaaaa tatcaacctc ttgaacaaaa
     5881 tccattttta ctcctcctca ttcctgcgaa gaatgtattt aggggtcagg tctcaacatt
     5941 tgacataaga aaggaatgtc aaatgttgag acctaacccc attattagac ttgctattct
     6001 ccccctttgg tgatgcagta gtaaccattt tcaaaccttt ttttctccca taccgggcag
     6061 gaatcacaga tacacccctg cctggtatta atgcacttac ttacttctcc aaccaaacaa
     6121 tatgccagcg ggtcaccgca atccagataa gaggggcaac ccggacagag acattgggcc
     6181 tgcaattcct tatcaacctc tttatttgcc atttcattca cctcctttac tatttaaaac
     6241 atgtcagggg acggtccttt aacatctttt tctttctgcc aatctgaaca gaaaattaaa
     6301 ttcagagaat atttttggaa ttgttcttgc agcagatatc tattcattat gtagatgttg
     6361 tatttatagc tgtgattggc agcaatcccc tttcattttt taaaagattt actttctata
     6421 aggattatct accagaacag atattgtaaa ataaaggcac tctatatttg tattgcattt
     6481 caatattttt caatttcctg attctgtttt agagcttcag aaatattaaa ttttaaaatg
     6541 acaatatttt gagcgaaaaa catgctgaag gtttttggta tgatttttgt gtgtatctga
     6601 tattatacag gcgataatat tattgtcaaa tcaagtaaaa aacgtctaaa aatattttag
     6661 taggattttt ttgacataat agatatggta tgacaaccaa tacttctttt tatttccatc
     6721 ttcatttcaa tttcttttta taatttaatg ggaatcatca aaattggtat aacaaatttt
     6781 cgttattcac ttcctgtatt ttctcgatat ctctttacaa ataaaaagtt aattcaataa
     6841 tttttttagt tttttgataa ttccatctcc aatctcagat gttgaagctt ttcccccaaa
     6901 ttctataggc ttaatctttc cttccttaag tacttcttgg gtaacatttt ctattgttga
     6961 tgcgatcttc gtctcaccta aagaatcgag catcattgct ccagctagaa ttgcagcaat
     7021 aggattggct ttaccctgat tttttatatc aggtgcagaa ccgtgaatcg gttcaaacat
     7081 tgatattcct tctgggttaa tatttgcact ggcggcaatg cctatacctc cttgaattgc
     7141 cgcacctaaa tcggtaataa tatcgccaaa catatttgga gtaactacaa tttgaaatct
     7201 ctctggatta gtaaccatca gcatggtcat ggcatctaca taataataat cagttgaaat
     7261 gtctggaaaa tcccgagaaa catctttaaa aactcttctc cataaaccat acatatcaga
     7321 taaaacatta gccttgtcaa cagtggaaac tttgctgaga cctttttttt cagccagttc
     7381 aaaggcataa cgaataattc tatctgaccc tttccgagtc aatagtccta gctgatatcc
     7441 gatctcttcc tctccgtcaa gttgaaagtc tatttgaata tttgattgaa aaagatttct
     7501 ttttataaca tggtcagaat gatactcctt acctttaaaa ctcgatccag ttccaatata
     7561 aaaatcttca gtattctccc taactacata aaaattaata tttttaaatg ttttatttct
     7621 caaaggactt tcaatcccct gtaataatct aataggcctt aaattaatat actggtctaa
     7681 agaaaatctg atttttagca agatctcttt ttctaagata ccgggtttga cttgaggatc
     7741 tccaactgct cccagataga ttgcatccat accttttatc tcctctaaaa cactatcagg
     7801 taacaattca ccttttttga gataatgttc ggcaccga
//
LOCUS       DAOQWT010000012         7746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_9825_length_7746_cov_3.168379, whole genome shotgun sequence.
ACCESSION   DAOQWT010000012 DAOQWT010000000
VERSION     DAOQWT010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9825_length_7746_cov_3.168379"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1476)
                     /locus_tag="PKJ95_00660"
     CDS             complement(151..1476)
                     /locus_tag="PKJ95_00660"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNR64783.1"
                     /translation="MKNSYKEAIKYIYNLNKYGIKLGLKNITCLLSLFDNPHLKVNII
                     HVAGTNGKGSASAITHSILQNAGFKVGLYTSPHLVSFQERMVINGEYITPEEVCSLLD
                     RLKPAINKVAHTRGCQHPTFFEVITTMAFLYFYEKKVDFAVMEVGLGGRMDATNVSQP
                     LVSVITHIDFDHMDRLGNTLAEIAGEKGAIIKQNTPVVSADQFPEAQEVIKKIAREKK
                     SPLYTYGEEFNTTLISSQLKGNYFNYGGIYNKINNLYVPLAGEFQLENTSLAIAATEL
                     LNDKGFLVTEDDIIRGVNNSRWPGRFEIISEKPLVILDGAHNPNGAKQFILNLKKLVP
                     DKKIIAVLGVFQDKDYMAILKTIVPHVNQVILTMADNPRATPIKILAEEVKNYIDDVN
                     IFETRKVSAAIKKAFQIARQDDVICITGSLYTVGEAKTYFLEEKNLKKG"
     gene            complement(1486..4155)
                     /locus_tag="PKJ95_00665"
     CDS             complement(1486..4155)
                     /locus_tag="PKJ95_00665"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNR64784.1"
                     /translation="MENEQDIKSDLSTVYNPKIVEDHWYQYWTVNNLFQVRNNGEKEK
                     NYSIVMPPPNITGSLHIGHALNNTLQDIVIRWKRMQGFNVLWLPGTDHAGIATQNVVE
                     REIAKEGKTRYDLGREKFLERAWQWREEYGSNILNQLKKMGCSCDWSRLRFTLDEGLS
                     KAVRKVFVVLFKEGLIYRSNYVINWCPRCQTALADIEVEQREENARLYYIKYPLKGSA
                     TEYITVATTRPETMLGDTAVAVNPDDERYKNYVGKIAILPLVGRELPIVADKYVDPEF
                     GTGAVKITPAHDLNDFELGKRHNLQEINIINDDGTTNNNAGKYAHLSIKQARERVLED
                     LQEQGYIEKIENYKHSLGHCYRCDTIVEPYLSQQWFVKMKELALPAIKAVQEGRTDFV
                     PERWSKVYFEWMYHIKDWCISRQLWWGHRIPVWYCQDCSEFIVEMEDPQECPKCHSKN
                     MVQDKDVLDTWFSSALWPFSTMGWPEETPDLKDFYPTNLLVTGFDIIFFWVARMMIMG
                     LKFMKELPFREVFINPLIKDIEGKKMSKSRGNVIDPLLVIDQYGADALRMTLASLTIQ
                     ANYIRLSKDKIETYRNFTNKIWNVSRFVITNLQNFNPLNIKEEELKFNIFDRWILAHL
                     NKCIKKVTEGLNNYKFSDSAMAIYEFTWNYFCDWYIELVKETLYQKENSIEKETTQYV
                     LWYVMENILKLLHPFMPFITEEIWQKIPHRGLSISISNWPLYQKESLKRNIEEKVTVI
                     QEVIKVVRNIKAEMNIPLTRKVDLHIKVTDNKKEEILKESLPYIKSLAHINSIFTSSA
                     LEKPEYSATGVLEDIEIFIPLKNVIDIEAEVKRLEHKLAKIEKELILINKKLNNADFI
                     EKAPKEVINREKEKMDELADIRDRVSRNLKTIK"
     gene            complement(4479..5753)
                     /gene="clpX"
                     /locus_tag="PKJ95_00670"
     CDS             complement(4479..5753)
                     /gene="clpX"
                     /locus_tag="PKJ95_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNR64785.1"
                     /translation="MKCSFCGKDQLEVGKMISGPDGVNICGECIQVCNYILSEEKIKK
                     EKQNFSIKNIPTPEGIKRYLDQYVIGQERAKKILSVAVYNHYQRIDNNLFRKDRKTGK
                     KTAKDFVKLDKSNILLLGPSGCGKTLLAKTLAEKLEVPFAIADATTLTEAGYVGDDVE
                     NVLLKLYHAAGEDIQSAEMGIIYIDEIDKITRKSENPSITRDVSGEGVQQALLKILEG
                     TSATMPVQGGRKHPHQKNIDIDTTQILFICGGSFEGLENIIKSRLQTRHIGFNADIFS
                     SEKKREKDKILRQVLPEDLIRFGLIPELVGRLTIIATVEDLDRNALMDILIKPKNALT
                     LQYKQIFQNQGVELKFTQSALEAVVEKAISRNTGARGLRSIIEESMLDIMYEIPSHKN
                     IKECTITDKVIKNNEMPIAIREKEKKQIKETA"
     gene            complement(5769..6368)
                     /locus_tag="PKJ95_00675"
     CDS             complement(5769..6368)
                     /locus_tag="PKJ95_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNR64786.1"
                     /translation="MYLVPIVVEKTPQGERSYDIYSRLLKDRIVFLGSPIDDQTANII
                     IAQLLFLEATDPEKDIHIYINSPGGSVSSTIAIYDTIQYLKSHVATTCLGMAASGAAL
                     ILASGTKGKRFSLPNSRIMLHQPLGGAQGQVSDIEIQTKELLRIKKKINEIIAYHTNQ
                     PLDRIEKDTDRDYFMTGDEAKEYGIVDEVIKKRSDIEKK"
     gene            complement(6382..>7746)
                     /gene="tig"
                     /locus_tag="PKJ95_00680"
     CDS             complement(6382..>7746)
                     /gene="tig"
                     /locus_tag="PKJ95_00680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNR64787.1"
                     /translation="KKSDRKEILRFYYDKWKLWRKESMFHIEKGKREENQIELKVTME
                     PDTVDEAFKEIYHEFSQKVKVPGFRTGRVPINILEMNLGKEYINQQVAEKLIKESYSK
                     AIEESELDPIDVPKVELIQIEKDKPFIYKIILELKPEFEIPALGDISLEKKQQEVTEQ
                     EIEAELEKLRESHAKLKAIENRESQMGDFLVLDFEAFLNGKSVENSKAEKQIIQLGER
                     VPPEFNENLTGVKPDEEKEIIVKYPDNVNDKKLAGKEITYKIKVLEIKEKELPELDDD
                     FAKSVGDYQKLDDLKVHIEKQLNEQAKFQTEREFQDLLMEKVSEKCIFEVPEVLIEKQ
                     LEHMFNNLKEDLKIRNMTLEEYYKLIKADEEKVRKEYRLIAEKQIKQELIIDKIIQDD
                     KITATEEEVNKKIEEIAESTNQKPLKVRAMFEKNKTLDNLKEQIKRDKAIAVLSEKIK
                     IIEK"
BASE COUNT         2120 a         1632 c         1201 g         2793 t
ORIGIN      
        1 attggtctca ctactttcct gagaggcaga tatgattgtg ataccttctt tttcctgaag
       61 gtacaatttc caaaagaaga atattatcac aacagagaca attaacaaca aaactatttt
      121 ttcctgttta gagaaaataa tcaaaactaa ttaccccttt tttaaatttt tttcttcaag
      181 aaaataagtt tttgcctcac ctacagtata cagtgaaccg gttatgcaga ttacatcatc
      241 ttgccgtgct atttgaaacg ccttctttat ggcagccgaa actttcctgg tttcaaagat
      301 attcacatca tcaatataat ttttgacctc ctcagctaag attttgatgg gagtagcgcg
      361 tggattatca gccatggtaa gaattacctg attaacgtga ggaacaatgg tttttaaaat
      421 ggccatataa tctttatcct gaaagacacc cagtacggca attattttct tgtccggtac
      481 caattttttt aaattgagaa taaattgttt ggctccattg gggttatgtg ctccatccaa
      541 tataaccagt ggtttttcac ttataatttc aaagcggcca ggccatcggc tattgtttac
      601 acctctgata atatcatctt cagttaccag aaaacctttg tcattgagca gttctgtggc
      661 agctatagcc aaagaagtat tttctagttg aaactcacct gctaggggaa catataaatt
      721 attaatttta ttataaattc ctccataatt aaaatagttt ccctttaact gagaactgat
      781 aagggtagta ttaaactctt ctccataagt atacaaggga gattttttct ctctggcaat
      841 cttttttatt acctcttgag cttcagggaa ctgatctgca cttaccacgg gtgtattttg
      901 cttaataata gccccctttt ccccggcaat ttcagccagg gtattaccca gtctatccat
      961 atggtcaaaa tcaatatggg taatgactga aacaagaggc tgactgacat tggtggcatc
     1021 cattcttccg cctaagccca cttccataac agcaaaatca acctttttct cataaaaata
     1081 gagaaaagcc atagtagtaa taacttcaaa aaaagtagga tgttgacagc ccctggtatg
     1141 ggcaacttta ttgatagccg gtttcagtct atccagtaga ctgcaaacct cctcaggagt
     1201 aatatattct ccgtttatta ccattctttc ctgaaaagaa accagatgcg gggaggtgta
     1261 taagcctacc ttaaacccgg cattttgtaa tatggaatgg gtaatggcag aggcagaacc
     1321 ctttccattg gtaccagcta catgaattat attaaccttt aaatgtgggt tatcaaaaag
     1381 tgagagtaaa caggtgatat ttttaagacc taattttata ccatatttat tgagattata
     1441 gatatattta attgcttctt tataactatt tttcatatac tattactatt ttatggtctt
     1501 caggtttcgg gaaaccctgt cccttatatc tgccaattca tccatctttt ctttttctct
     1561 atttataact tctttaggcg ctttttcaat aaaatcagca ttgtttaatt tcttattaat
     1621 aaggattaat tccttttcaa tctttgctaa tttgtgttct aatcgcttca cttctgcctc
     1681 aatatcaata acattcttta gtggaatgaa aatttctata tcctctaaaa caccggtagc
     1741 agaatattct ggtttttcta aagcactact cgtgaagatg gaattgatat gtgccaaact
     1801 cttgatatag ggcaagcttt cctttaaaat ttcttctttt ttattatctg ttactttgat
     1861 atgcaaatcc actttcctgg ttaaaggaat attcatttca gctttaatat tgcgaactac
     1921 cttaataact tcctgaatga cggtaacttt ctcttcgata ttccgcttaa ggctttcttt
     1981 ttggtataga ggccagttgc tgatagagat gctcagtcct ctgtgaggaa ttttctgcca
     2041 gatttcttca gttataaaag gcataaaagg gtgtaatagt tttaaaatgt tttccataac
     2101 ataccagaga acgtattggg tagtctcttt ctcaatactg ttttcttttt gatacaatgt
     2161 ttctttcacc agctctatat accagtcaca aaaataattc caggtaaact catatattgc
     2221 catagctgaa tcactaaatt tgtaattgtt cagtccttct gttacttttt tgatgcattt
     2281 attcaaatgg gccaatatcc aacggtcaaa tatattgaat ttcagttctt cttcctttat
     2341 attcaatgga ttgaaatttt gtagattagt tattacgaag cgggacacgt tccatatttt
     2401 attagtaaaa ttgcggtatg tttcaatctt atcttttgat aaacgaatat aatttgcctg
     2461 aatggtcaga gaggccaaag tcatccgtaa ggcatcagcg ccatactggt caatcaccag
     2521 caaggggtca ataacattgc ccctcgactt actcatcttc ttcccttcta tatctttgat
     2581 gagagggttt ataaatacct ccctaaaggg caattctttc ataaatttaa gccccattat
     2641 catcattctt gccacccaga agaaaataat atcaaatccg gttaccaata gattggtggg
     2701 ataaaaatct ttcagatctg gtgtctcttc aggccagccc atagtcgaaa agggccatag
     2761 ggctgaacta aaccaggtat cgaggacatc tttatcctgt accatattct tgctatgaca
     2821 tttcgggcat tcctggggat cttccatctc cacgataaat tctgagcagt cctgacaata
     2881 ccatacagga attcgatggc cccaccataa ctgtcttgag atgcaccagt ctttaatatg
     2941 gtacatccat tcaaaataga ccttggacca tctttccgga acgaaatcag tccgcccctc
     3001 ctggactgcc ttaattgccg gcaaagccag ttctttcatt ttaacaaacc attgctgaga
     3061 gaggtatggt tcaacaatgg tatcacagcg ataacagtgg cctaaggagt gtttatagtt
     3121 ttctatcttt tcaatatatc cctgttcctg caagtcttcc agtactcttt ctctggcttg
     3181 ttttatagaa agatgggcat acttgccagc attattatta gtagtgccat catcgttaat
     3241 tatatttatc tcctgcaggt tatgtctctt gccaagttca aaatcgttta aatcatgagc
     3301 cggggttatc ttcacggcac ctgtcccaaa ttccgggtca acatatttat cagcaacaat
     3361 gggtagttcc ctacctacca gaggcagtat agctatttta ccaacataat ttttatacct
     3421 ttcatcatca gggtttaccg caacggcagt atccccaagc atagtctcgg gtctggttgt
     3481 agcaacagta atatactctg tagctgaacc tttcaaggga tattttatat agtataacct
     3541 ggcattttcc tctctctgtt ccacttcaat atctgctaaa gcggtctgac agcgggggca
     3601 ccaattaata acataattag aacgataaat cagtccttcc ttaaaaagaa ctacaaatac
     3661 ctttcttact gctttagata atccttcatc aagggtaaat cttaaacggg accaatcgca
     3721 ggaacatccc atttttttaa gctggtttaa gatattagaa ccgtattctt ctctccattg
     3781 ccaggcacgt tctaaaaatt tttccctgcc cagatcatat ctggtctttc cctctttagc
     3841 tatctctctt tccactacat tctgggtagc tattccggcg tggtctgttc ccggaagcca
     3901 taaaacattg aatccctgca tccttttcca gcgtatcaca atatcctgca gggtattgtt
     3961 cagggcatga ccaatatgca gtgaaccggt aatattggga ggcggcatta cgatagaata
     4021 atttttttcc ttctctccat tatttctaac ttgaaaaaga ttattaactg tccagtactg
     4081 ataccagtgg tcttctacta tttttgggtt ataaaccgtt gataaatcac tttttatatc
     4141 ttgttcattt tccataggaa tttctcctca gaattattaa aaaacccttt ccatccttca
     4201 taaggacgaa aagggttcgt ggtaccacct taattcctgt tctttatact tgttaattta
     4261 agttatagaa caggctcttc taatagtgtt aacggttctg ccgttgaaac ttaccctcgt
     4321 ttcagtaaaa ggttccggga tgactttcaa tagaaccatt taaccggttt atttactctt
     4381 cccttcaata ccaatcacta atagatttat ttgctctata ttactatcaa tattttagga
     4441 tgtcaagcaa aaaagcgata aagataagtt tttgattatt aagcagtttc ttttatttgc
     4501 ttcttttcct tttctctgat agcaatgggc atttcattgt ttttaataac cttatctgtt
     4561 atggtgcatt cttttatatt cttatgggaa ggtatctcat acataatatc aagcatagat
     4621 tcttcaataa tcgaccttaa tccccgggca ccagtgtttc tgcttatggc cttctccacc
     4681 actgcttcca gggctgactg agtgaatttc aactcaactc cctgattttg gaaaatctgt
     4741 ttgtactgca aggtaagggc atttttaggt ttaattaaaa tatccattaa agcatttcta
     4801 tcaaggtctt caacagtagc aatgatggtc agtctgccta ctaattccgg tattaaacca
     4861 aatctaatca gatcttccgg taaaacctgt cttaaaatct tatctttttc ccgcttcttt
     4921 tcactgctaa aaatatctgc attaaaacca atatggcgag tctgtaatcg gcttttgata
     4981 atattttcca atccttcaaa ggaacctcca cagataaata atatctgggt ggtatcaata
     5041 tcaatatttt tctgatgcgg atgctttctt cctccctgca cgggcattgt tgcactagtt
     5101 ccttccagta ttttcaataa agcctgctgt acaccttctc cggatacatc acgggtaata
     5161 gagggatttt ccgactttcg ggtaatctta tctatttcat cgatatagat aatacccatt
     5221 tctgcacttt gtatatcttc acccgcagca tgatataact tcagcaatac attttccaca
     5281 tcatctccca cataaccggc ctcggtaagg gtagtagcat cagctatagc aaagggaact
     5341 tctaactttt ctgctaaagt cttggctaaa agtgtttttc cgcaaccact gggacccagg
     5401 agcaagatat tgcttttatc caattttaca aagtcttttg ctgttttctt gcctgttttc
     5461 ctatcctttc tgaaaagatt attatctatt ctctggtaat gattatagac agctacggaa
     5521 agtatttttt ttgccctctc ctgccctatc acatattgat ctaaatacct ttttattccc
     5581 tcaggagtag gaatattctt tattgaaaaa ttctgttttt cttttttaat tttttcttca
     5641 cttagaatgt agttgcaaac ctgaatacat tcaccgcata tattaactcc atcgggtcct
     5701 gaaatcatct ttccaacttc aagctggtct tttccgcaaa acgaacattt cattctgaac
     5761 tcctcaagtt atttcttttc aatatcactg cgtttcttaa taacttcatc cacaatcccg
     5821 tattctttgg cttcatctcc ggtcataaaa taatcacggt cagtatcttt ttcgattcta
     5881 tccaaaggct gattggtgtg ataggctatt atctcattta ttttcttctt gattctcagt
     5941 agctccttag tctgaatctc aatatcagat acctgtccct gggcacctcc taatggctga
     6001 tgcagcataa ttctggaatt gggcaaagaa aatctcttcc ccttagtacc agaggctaaa
     6061 atcaaggcag cgccgctggc agccatacca aggcaggttg tagcaacatg ggatttaaga
     6121 tactgtatgg tatcataaat agcaattgta gaacttaccg agccgccagg actgttgata
     6181 tagatatgaa tatccttttc cgggtcagta gcttccagaa acaacagctg tgcaattatt
     6241 atgttagcgg tctgatcatc aataggactg cctaaaaata caattctatc ttttaacaac
     6301 cgcgagtata tatcatagga tctttccccc tggggagtct tctccaccac tatgggaact
     6361 aaatacattt tggattcctc cttatttttc aattattttt atcttttctg agagtaccgc
     6421 aatggcttta tctcttttta tctgttcttt cagattgtcc agagtcttgt ttttttcaaa
     6481 cattgctctc acttttaacg gtttttgatt ggtactctct gcaatttcct caattttttt
     6541 gtttacttct tcttcagtag ccgtaatttt atcatcttgt ataattttgt caataatgag
     6601 ttcctgtttt atctgttttt cggcaattaa gcgatattct ttcctgacct tttcttcatc
     6661 agctttgatt aatttgtaat actcctccag ggtcatattt cttattttta aatcttcttt
     6721 caaattattg aacatatgtt ccagttgttt ttctatcagt acttcaggaa cttcaaaaat
     6781 acatttttca gagacttttt ccattaatag gtcctgaaat tctctttccg tctgaaattt
     6841 tgcctgttca tttaattgct tttcgatatg aacctttaaa tcatccagtt tttgatagtc
     6901 accaacactt ttggcaaagt catcatctaa ttccggcaat tccttctctt taatttctaa
     6961 tacttttatc ttataggtaa tttcttttcc tgctaatttt ttatcattta cattatcagg
     7021 atattttact attatttctt tttcctcatc tggttttaca ccagtcagat tttcattgaa
     7081 ttctggcggt accctttccc caagctgaat tatctgtttt tctgctttgc tgttctctac
     7141 tgatttgcca ttaagaaaag cctcaaaatc cagtaccagg aaatccccca tttgtgattc
     7201 tctgttttct atcgccttta atttagcatg gctttccctg agcttttcca attctgcctc
     7261 tatctcctgt tcggttactt cttgttgttt cttttccaga gaaatatcac ccagagcagg
     7321 tatttcaaat tcaggcttta attctaagat gattttatag ataaagggtt tatccttttc
     7381 aatctgtatt aactcaactt tgggaacatc aatagggtcc agttcactct cttctatggc
     7441 tttactgtaa ctttctttta tcagtttttc agcaacctgt tgatttatat attctttacc
     7501 taaattcatc tccagtatat taatgggcac tctgccggtg cgaaaaccgg gaaccttaac
     7561 cttctgagaa aattcatggt atatttcctt aaacgcctca tcaactgtat ctggctccat
     7621 agtaactttt aattctattt gattctcttc cctcttacct ttttcaatat gaaacatgct
     7681 ttccttcctc cacagcttcc acttatcata ataaaaccgc aaaatttctt tgcggtcaga
     7741 tttttt
//
LOCUS       DAOQWT010000013         7718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_9873_length_7718_cov_3.474096, whole genome shotgun sequence.
ACCESSION   DAOQWT010000013 DAOQWT010000000
VERSION     DAOQWT010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9873_length_7718_cov_3.474096"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1537
                     /locus_tag="PKJ95_00685"
     CDS             50..1537
                     /locus_tag="PKJ95_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64788.1"
                     /translation="MKMKRELWLTIGMVIVNILFISFFTVLAVESPPQLIPLEELPEE
                     TPEIIALREKLEAYIDDYTSDMIEMSEHLYHNPEPGYYEYESSKYVAAELEKYGFEVE
                     FGVEGLDEEYNTVIEKLHNARNLPVAFTAKYKGKSEYPVIAFTTEMDALRGNPPFHGC
                     AHNQQPPVALGAAIALTKIMDENNIPGSIWVIHLPAEEIPPPDPTAMFNAGYFDEVDF
                     VLQTHGGVNSGVFNRPLAGTGAAQLINANIYEFRGKTAHSAGDPWNGRDAGDAVRAMW
                     MMIDMIREHSRPEFRFHGAIVETAVAPNSINDRVVFDHWVRANPGLSKEDLNQKVEQI
                     NTIAKAAAMGTFTDVEISHYADYGSGVTTAWANALGWYYTKMYSDEGMTSDEPGRQAS
                     GWDVGQWACWSIPGIGSERGDWTIAMADVPPTAGHSQEKADLTITPLGHRSLPLMAKR
                     QAAVALRLVTQPELLVKINEELAQWREYGLEKGYVTEDVFRLKNK"
     gene            1741..2493
                     /gene="mobB"
                     /locus_tag="PKJ95_00690"
     CDS             1741..2493
                     /gene="mobB"
                     /locus_tag="PKJ95_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNR64789.1"
                     /translation="MKAIGIVGFKKSGKTTLALKIARALTNKNYRVAVIKHSSQSVCK
                     EESDTNRFLKEVEKVALMTPNSAEILFNSKQGLKKIISLLSADFLVIEGFKSLKHFPK
                     IICLREESEKELLSDGLELFSAGMDVSLKEKKVIDFLITDENVLKTMIEEIEQKSFLL
                     PDENCGKCGYGDCYGLARAIVKGQETKESCSYLHDFFSLKVNGKEVSLNQFMSKLYQS
                     LIYGMLAPLKDIDPLEESEIEIKVNLSGYNKK"
     gene            2570..3526
                     /gene="trxB"
                     /locus_tag="PKJ95_00695"
     CDS             2570..3526
                     /gene="trxB"
                     /locus_tag="PKJ95_00695"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNR64790.1"
                     /translation="MVKEQENPQKNIVDTIIVGGGPAGLTAGLYLSRYRIETILFEKG
                     ISGGQVNLSEVIENYPGFPEGIIGPDLMQRFEQQAIKFGLKIINSTVLTVSPEKYENK
                     TNFKISTADGIFYSKAVIIASGGEPNKLGVPGEQELTGRGVSYCGTCDGAFFREKEIA
                     IVGGGDTALEEALFLTKFASKITIIHRRDELRGSKILQERVFNHPKIKFSWSTIVLAI
                     NGETQVKSLQLKNVQSGEINTFPCQGIFIFTGYRPAYPSFGEMQKQLINENGYIITDG
                     NMKTAIEGIFACGDVRAKGLRQIVTACGEGATAAFFVEALLK"
     gene            3596..4426
                     /locus_tag="PKJ95_00700"
     CDS             3596..4426
                     /locus_tag="PKJ95_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNR64791.1"
                     /translation="MNIDFGISTLAYYQKPIEKLLPCLVKEKINNIEIRLKENHFLPQ
                     EINELLKLKKLLNQKRIVVKAIHMPMNGVDISHPKNYERIKSVREVEKVVLMAFHLGA
                     ELIVVHPGAMVNNAEDKKNRLRLSMESLKEIMDFNQNWNIKIALENMLPGRVGDKWED
                     IQLILQNVSSKNLGICFDTGHYLLNFPEIEKGKLNLDKMGINWPNSLLHIHLHDNDGK
                     RDLHLLPQEGLFPWTSLISFLKTIKYEGIFIFESKEQNNINDYLSRVNTVWYNIRNLI
                     "
     gene            4535..6637
                     /gene="fusA"
                     /locus_tag="PKJ95_00705"
     CDS             4535..6637
                     /gene="fusA"
                     /locus_tag="PKJ95_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNR64792.1"
                     /translation="MTKNDVEKVRIFALVGHGDSGKTSLTEAMLYDSNMTNRLGRIEQ
                     GNTITDYDSKEIKRGITINSSLAYLNWKNSTFNIIDTPGYLDFIIDTKSSLRVVDCAL
                     IVVCGVSGVEVQTEKVWDYAEEFQLPRVFFINKMDRERADFERVSEKIKENFGQNAVP
                     VQLPIGAEENFEGVVDLLKMKAYYFQPGKDGKPEMEEKEIPENLMGKAETYHQKMVES
                     VAEFDDEILMKYLDGEKLTEEEILTCLKGAMIERKVFPILCGSATKNIGIELLLDFIE
                     GYFPNPIELPDIMVKSLKEQKDIPLKRAVDGAPFAALVFKTIADPYVGNLTYFRVFSG
                     ELHSDSQIYNSTQDVENKVGKIFRMQGKEQSPVSKVSAGEIGAIAKLKKTNTGDTLCD
                     KEFPVEFEKIEYPEPIMRLAIFPKSKGDEDKLSIALSRIQEEEPTIQIFRDEDTGETI
                     IAGMGEPHLDVVIETMEKKFGVEVEKNTPKVGYKETIRKKVQAEGKYKKQSGGRGQYG
                     HCFVEFEPLERGKGFEFVDKIVGGVIPKQYRPAVEKGIVESMKKGVVAGYPTIDIRAT
                     LYDGSYHTVDSSEMAFKVAGSLAFKKGAAMADPLLLEPICNLEVIVPKDYMGDIIGDL
                     NSKRGKIMGMDEGEKGRQIIKALVPQAEMFRYSIDLRSITQGRGTFSLKFSHYEEVPP
                     QIAQEIIEKAKQESESES"
     gene            7348..7423
                     /locus_tag="PKJ95_00710"
     tRNA            7348..7423
                     /locus_tag="PKJ95_00710"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7381..7383,aa:Ala,seq:ggc)
     gene            7534..>7718
                     /locus_tag="PKJ95_00715"
     CDS             7534..>7718
                     /locus_tag="PKJ95_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNR64793.1"
                     /translation="MKEKYDFKETEQKWQKFWYSRELFENDMVQGKKKYYVLEMFPYP
                     SGEPHMGHVKNYVIGDVV"
BASE COUNT         2653 a         1091 c         1678 g         2296 t
ORIGIN      
        1 attattttaa acaaatagaa aaaactaaaa gaaagatgag gaaatgttta tgaaaatgaa
       61 aagagaatta tggttaacaa taggaatggt aatagtaaat atactattta ttagtttttt
      121 caccgtattg gcagtcgagt cacctccaca gttgattccc ttagaagaac ttccagaaga
      181 aacaccggaa attattgctt taagagaaaa gttagaggct tatatagatg attatacttc
      241 tgatatgatt gagatgagtg aacacttata tcataatcct gaacctggtt actatgagta
      301 tgaatcttct aaatatgttg ctgctgagtt agaaaaatat gggttcgaag tggagttcgg
      361 tgttgaaggt ttggatgagg aatataatac ggtaatcgaa aaattacaca atgccagaaa
      421 tttacctgtt gcttttactg caaaatacaa aggtaagtca gagtatccgg ttattgcttt
      481 tactaccgaa atggatgcct taagaggtaa tcccccattc catggctgtg ctcataacca
      541 gcagccgccc gtagcccttg gtgctgctat tgccctaact aaaattatgg atgaaaacaa
      601 tatacccggt agtatctggg taatacatct accggcagaa gaaatccccc ctccagatcc
      661 aacagctatg tttaatgccg gatattttga tgaagtggat tttgttcttc aaactcacgg
      721 tggtgtcaat tctggagtct ttaatagacc tttagctgga actggagcag ctcagctgat
      781 taatgctaac atttatgaat ttaggggcaa gacagctcat tctgctgggg atccctggaa
      841 tggaagagat gctggagatg cggtcagagc aatgtggatg atgatagata tgataaggga
      901 acattccaga ccagaattca gattccatgg agctatcgtt gaaacagctg tagcaccaaa
      961 ttctataaat gaccgtgttg tttttgatca ttgggtcaga gcaaaccctg gcttgagtaa
     1021 agaagattta aatcaaaaag ttgaacagat aaatactatc gctaaagcgg ctgcaatggg
     1081 gacctttact gatgttgaaa taagccacta tgctgattac ggtagtggag taactactgc
     1141 ctgggctaat gccttagggt ggtattatac taaaatgtac agtgatgagg ggatgacctc
     1201 tgatgaacca ggcaggcagg ccagtggctg ggatgtagga cagtgggctt gctggagtat
     1261 tcctgggatt ggttcggaaa gaggtgattg gaccatagct atggctgatg tacctccaac
     1321 tgcgggtcat tcacaagaga aggcagatct tactattact cctttggggc atagatcttt
     1381 accgttaatg gcaaaaaggc aggctgcggt agctttgcgt ttagtaacac agccagaact
     1441 tttagttaaa ataaatgaag agctggcaca atggcgtgaa tatggactag aaaaaggata
     1501 tgttacggaa gatgtattta ggctgaaaaa taaatagaag attaagctaa aaatggattg
     1561 gatgatgaat ttgaaatcat catccaatcc aataataatc attcaaattt cttttaaata
     1621 ttcttcttat ttgccttctt attcccactt aaaaacctaa ccaattacga aataatttgc
     1681 agcttgtcat ttaatttgcc ttttgatata gtaatatcga ttatatcaag ggaggattat
     1741 atgaaagcta ttggaattgt tggttttaaa aaaagcggga aaacaacttt agcattaaaa
     1801 atagctcgag ctttaacaaa taagaattat cgagttgcag taataaagca ttccagtcaa
     1861 tctgtttgta aagaagagag tgatacaaac agatttttaa aagaggttga aaaagtagca
     1921 cttatgactc cgaatagtgc cgagatattg ttcaattcca aacagggttt gaaaaagatt
     1981 atatccctac tatctgctga ttttttggta atagaaggat ttaaaagcct gaaacatttt
     2041 ccaaaaatta tctgtttaag agaggaaagt gaaaaagaat tgctttctga tggactggaa
     2101 ctgtttagtg ccggaatgga tgtttcttta aaagaaaaaa aggttattga ttttttgatt
     2161 acagatgaaa atgttttaaa aacaatgata gaagagatag aacagaaaag ttttttatta
     2221 cctgatgaga attgtgggaa atgtgggtat ggtgattgtt atggtttagc ccgagcaatc
     2281 gttaagggac aggaaacaaa agaaagttgt tcctatctcc atgatttctt ttctcttaag
     2341 gtaaacggca aagaagtttc ccttaaccaa tttatgtcaa aattgtatca gagtttgata
     2401 tatggcatgt tggctccttt aaaagatatt gaccctctgg aagagtcgga aattgaaata
     2461 aaagtcaacc tttcggggta taataaaaaa tagaagtttt attagatgga gcaaaaggca
     2521 ttaaaacgtc gtattaagta atatttgcaa actaaggaga tgagttaaag tggttaaaga
     2581 acaagaaaat ccacaaaaaa atattgttga taccattatt gtaggaggag gtcctgcagg
     2641 attaacagct ggtctttatc tcagccgtta ccgtattgag acgatactat ttgaaaaagg
     2701 aattagtggg gggcaggtaa atttatctga ggtaatagag aattatcctg gattcccgga
     2761 gggaattatt ggaccggatt tgatgcaaag atttgagcag caggcaataa aatttggttt
     2821 aaagattata aatagtacag tactgacggt atctccggaa aagtatgaga ataaaacaaa
     2881 ctttaaaatc agcactgctg atggcatttt ttattctaaa gcagtcatta tagcctctgg
     2941 tggtgagcca aataaattag gggttccggg agaacaggaa ttgacgggaa ggggtgtttc
     3001 ctattgtggt acttgtgatg gagccttttt ccgcgagaaa gagattgcca tagtaggagg
     3061 aggggacact gccttggaag aggctctctt tctgaccaag tttgccagta aaataactat
     3121 tattcatcgt agagatgaat taagaggttc taaaatactc caggagcgtg tctttaacca
     3181 tcctaaaata aaatttagct ggagcactat tgtactggct ataaacggag aaacacaggt
     3241 gaagagctta cagttgaaaa atgttcagtc tggtgagatt aatactttcc cctgtcaggg
     3301 aatcttcatc tttaccggat acagaccggc ctatccttct tttggagaaa tgcagaaaca
     3361 gttaattaat gagaatggat atattattac tgatggcaat atgaagactg caatagaagg
     3421 tatttttgcc tgcggagatg tacgagccaa aggtttgcgt cagattgtta ctgcttgtgg
     3481 cgaaggagct actgctgctt tttttgtaga ggcattgtta aagtaaatag cggtaaataa
     3541 aagaatgaga agttagccat acttacattt tttatttacc ttaaatggag aaaaaatgaa
     3601 tattgatttt ggcatctcta ccctggctta ttatcaaaag ccaatcgaga aattattgcc
     3661 ctgtttagtt aaagagaaga ttaataatat tgaaattaga ctgaaagaaa accattttct
     3721 tcctcaagaa attaatgaac tcctcaaatt aaaaaagctg ttaaatcaga aaagaatagt
     3781 agttaaggct atccatatgc ccatgaatgg cgtcgatatt tcccatccca aaaattatga
     3841 gagaataaaa tcagtcagag aggtggaaaa agtggtgctg atggctttcc atttaggagc
     3901 ggaactgata gtggttcacc ctggagcaat ggtgaataat gctgaagata aaaagaatag
     3961 attaaggtta agtatggaaa gcttaaaaga aattatggac tttaaccaga attggaatat
     4021 taaaattgca ctggagaata tgcttccggg aagagtggga gataagtggg aagatattca
     4081 gctgatttta caaaatgtat cctctaaaaa tttaggaatt tgctttgata ccgggcatta
     4141 tctactcaat tttccagaga tagagaaggg gaaattgaat ttagataaga tggggattaa
     4201 ctggccgaac agcttgttgc atattcatct tcatgataat gatggtaagc gtgatctaca
     4261 tcttttacca caagaaggac tcttcccatg gacttcactt atctcttttt taaaaacaat
     4321 taagtatgag gggattttta tttttgaatc gaaagagcaa aataatataa atgattatct
     4381 tagcagggtt aatactgtct ggtataatat ccgaaacctg atttaaattt ttaaatttaa
     4441 aaggatagaa gatttaatta tgacaatttt tgctaaaaat agtattcttt gatagaataa
     4501 tgacattaat ctgattttca aggaggaatc tttggtgact aaaaatgatg tagaaaaggt
     4561 gagaatattt gccctggtcg gacatggtga ttccgggaaa acctctctaa ctgaagctat
     4621 gctttacgat tccaatatga ccaatcgttt aggaagaatt gaacagggta ataccattac
     4681 agattatgat tcaaaggaaa tcaaaagagg cattacaata aattcatctt tagcttattt
     4741 gaattggaag aattcaactt ttaatataat tgatacacct ggttatcttg attttattat
     4801 tgatactaag tcaagtttga gagttgttga ttgtgcactg atagtggttt gcggtgtatc
     4861 aggagtagaa gttcaaacag aaaaggtatg ggattatgca gaagaatttc agctacccag
     4921 agtattcttt atcaataaaa tggacagaga aagggcagat ttcgagcgtg tcagtgaaaa
     4981 aattaaagaa aactttgggc aaaatgctgt tccggtccag ttgccaatag gggctgaaga
     5041 aaactttgaa ggcgtggttg acctattaaa aatgaaggca tattattttc aaccaggcaa
     5101 agatggcaaa ccagagatgg aagaaaagga gatacccgaa aatttaatgg gaaaagctga
     5161 aacgtaccat caaaaaatgg ttgaatcagt tgcagaattt gatgatgaga ttcttatgaa
     5221 atatcttgac ggggaaaagc taactgagga agaaatactt acctgtttaa aaggcgcaat
     5281 gattgaaaga aaagtattcc cgattttatg tggctcggca actaaaaata tcggtattga
     5341 attactgctt gattttatag agggttattt ccccaacccc attgaattac cagatattat
     5401 ggtgaaaagc cttaaagagc agaaagatat tccactaaaa cgtgccgtag atggagcacc
     5461 ctttgctgca ctggtattta aaaccattgc tgatccatac gtagggaact tgacctattt
     5521 tagagtcttt tctggtgaat tacattctga ttcacaaatt tacaattcaa ctcaggatgt
     5581 tgaaaataag gttgggaaaa tatttcgtat gcagggcaag gaacaaagtc ctgtttctaa
     5641 agtgtctgcc ggggaaatag gggcgattgc caaattgaag aaaactaata ccggtgacac
     5701 cttgtgtgac aaagagttcc ctgttgaatt tgagaaaata gagtatcccg aacctattat
     5761 gagattggca atttttccaa agagtaaagg ggacgaagac aaactgagta ttgccttaag
     5821 cagaattcag gaagaagaac caaccattca gattttcaga gatgaagata ccggggaaac
     5881 gattattgcc gggatgggag aaccacatct tgatgtggtg atagaaacaa tggaaaaaaa
     5941 atttggggta gaagttgaaa aaaatactcc taaagttggc tacaaagaga ctattcgcaa
     6001 aaaagtacag gccgaaggca aatacaaaaa acaatccggt ggtcgtggtc aatatggaca
     6061 ttgttttgtt gaatttgaac cactggaacg cggaaaaggt tttgaattcg ttgataaaat
     6121 tgtaggtggt gttattccaa aacagtatcg tccagctgta gaaaaaggga ttgttgaatc
     6181 tatgaaaaag ggtgttgtag ccggttatcc tactattgac ataagggcta ctttgtatga
     6241 tggaagttat catactgtgg attcctctga aatggcgttc aaagtagctg gttctcttgc
     6301 ttttaaaaaa ggtgccgcaa tggctgaccc attactgtta gaaccaatat gcaatctgga
     6361 agttatagtt ccaaaagatt atatgggaga cattattgga gatttaaata gtaaaagggg
     6421 taaaataatg ggtatggatg agggtgaaaa aggtagacaa attattaaag cactggtacc
     6481 ccaggctgaa atgttcaggt attctattga tttgcgctct attacccagg ggagagggac
     6541 attttctttg aaattttctc attatgaaga agttcctcca cagattgctc aggaaattat
     6601 tgaaaaagca aaacaagaaa gtgaatccga atcataatta ttaataaaat tacatatatc
     6661 ctaattttag aaatatttta aacaaatact tgtcttcaag gcacttggta actataataa
     6721 gataagactt ataaaaattt attaaatgag gaggaggtgt aagtaaatgt ttggagattt
     6781 agaatggtac tggattgcgg caattattgc ggccattgtc ggagtaattt ggtggctggg
     6841 caaagaataa aatattttag aaggattcat aaaaagtttt tgtagtaatc gtaataatgc
     6901 cagaataagt gcaggatttt gctattttta ttgattataa caacctgcac ttattttttg
     6961 caatttgatc tgattgagtc gattactgaa ttgtttcatc gcttgtttta attgactttt
     7021 aaatttcatt ttattataat taaaaaaatt taaaataata aaagatttaa tttttcataa
     7081 aattcgactt gtttattagt acaaataata tatactaaat tattgtataa ggatttaata
     7141 ctaagctaaa aattctttaa gatatattat aattttaaaa aagacattaa gaacaggaaa
     7201 attattggag tacctattta aagagagtca gtgtctggtg taaactgatt aaggttccca
     7261 aaaaactcac ctgaaaattt cttgagtgaa aataacaaat tgatagctca agacgatgtt
     7321 ctgcgttaaa gaagaagaag ggtagatggg gctgtagctc agacgggaga gtgcctcctt
     7381 ggcgtggagg aagtcgcggg ttcgattccc gccagctcca ccatttttta tctactaaag
     7441 aagggtggta ccacgagtaa atctcgtccc tggtaatttt attattacag gtggcggggt
     7501 ttttctattt taggggagtg agatgataag actatgaaag agaaatatga ttttaaagaa
     7561 actgaacaaa aatggcaaaa attctggtat agtcgggaac tatttgaaaa tgatatggta
     7621 caaggtaaga aaaaatacta tgttctggaa atgtttccct atccttccgg agagccccat
     7681 atgggacatg ttaagaatta tgttatcggt gatgtggt
//
LOCUS       DAOQWT010000014         7484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_10266_length_7484_cov_3.377709, whole genome shotgun sequence.
ACCESSION   DAOQWT010000014 DAOQWT010000000
VERSION     DAOQWT010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10266_length_7484_cov_3.377709"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PKJ95_00720"
     CDS             <1..913
                     /locus_tag="PKJ95_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNR64794.1"
                     /translation="NTALRCIGDARSPMLIMILTSLLNIVLDPFLMFDIIPGTSITGM
                     GLGIKGAAIATVISTVIAFSIGLFILLSGKSFIKISFSGLFKLSKENAKKLIIIGLPT
                     GLEMFFRQGSQFLLLKFVSFYGTLALATFGIGMKLFNLVFLPLLGLSMGGSAVAGQNL
                     GANHIERTRQTAIWTSLLGIIITGIVSSLALLFPEAILKIFINQKEVIEIGRKMIYIL
                     SPSFIAVAISMGLNTVFAGSGYNLPYLISGIVSRWFFQIPFSALIIYIFHLPITYIWV
                     GFFIAEFVELLVIYTFYHRGRWKTTRV"
     gene            complement(915..1373)
                     /locus_tag="PKJ95_00725"
     CDS             complement(915..1373)
                     /locus_tag="PKJ95_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR64795.1"
                     /translation="MEQDTSILLSLIKELAVFEKLADKVINSEEAIRETLFGPKAYAE
                     AEIAYWENEPVGMVLFFHNYSTFLGKPGLYIEDLFVREKFRGKGVGKALFLECVRLAR
                     ERKCGRMEWVVLDWNPAREFYEKMGAYPLTDWVIYRMDEKTLENIGLQPR"
     gene            1669..2115
                     /gene="mscL"
                     /locus_tag="PKJ95_00730"
     CDS             1669..2115
                     /gene="mscL"
                     /locus_tag="PKJ95_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNR64796.1"
                     /translation="MLEEFKKFIMRGNVIDMSVGIVVGAAFGAIVSSFVNDILMPPIG
                     LLLGGFDFSNLFILLKQGTTPGPYLTLAQAQETGAVTLNYGVFMNAIISFLIIALAIF
                     LVIRQINRLKKAEEAPPPSTKECPFCFTVIPIKAKRCPNCTSELAQ"
     gene            2868..3428
                     /locus_tag="PKJ95_00735"
     CDS             2868..3428
                     /locus_tag="PKJ95_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64797.1"
                     /translation="MAILKNFKESICVTVITFILTIIVALISYSWMETISFFPAVLIL
                     IIIISIGVLSDMVAFAAPAAEVESFHAKAAKKVFGAKKGLFLVKNAHRVANFMGDIVG
                     DICGILSGSLGTIIVIKTASNWQAPKSWIDLLVLSLIAAITVGGKSFLKSYALRQANE
                     IILFAGKILASPTLLKNIFKKIKNNS"
     gene            complement(3871..4521)
                     /locus_tag="PKJ95_00740"
     CDS             complement(3871..4521)
                     /locus_tag="PKJ95_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64798.1"
                     /translation="MENIRIFEQTAPLLAIILIILAFCLAIIFYIWSLRVLKKIESQI
                     IRMKQNVTDLENLSQYIYETAYFDMKDNINKGKNVDGTSLDFRISLDEFKELKSQIQH
                     LEERQTEINQISYQKEEKKLEKQKEIPSNKNSAFMPSLQPDEEDKYEKISELIVMYLH
                     DLLQEKEQVTAQELVYTMPNKYSLADIYRTLEIMKERGKINWEDKSIHPKSFLILP"
     gene            complement(4554..4925)
                     /locus_tag="PKJ95_00745"
     CDS             complement(4554..4925)
                     /locus_tag="PKJ95_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64799.1"
                     /translation="MNYSELITIALSFLSIFICLSTIWFAILFYRLYTEFSLMITEMS
                     KGLEISAIRLERIPKILYDKELILPKPTHPQNLIIAEKEKEVVSSNQEDVAQKSEMRT
                     DYHEKQDKDLLKQIPKESEEI"
     gene            complement(4946..5707)
                     /locus_tag="PKJ95_00750"
     CDS             complement(4946..5707)
                     /locus_tag="PKJ95_00750"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013776689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNR64800.1"
                     /translation="MTATNQQEIKKVSELLQKSEYTVCLTGAGVSTASGIPDFRTPGK
                     GIWSKINPIEVTSIDAFRENPARFYHFYRPRIEELKNVVPNQAHYALVRLEQSNYLQK
                     IITQNIDNLHQKAGSQNVLEIHGNLSQAICNRCGAKISSQLLIEKIIENIQQIPYCNC
                     GGILKPDVVLFGEMLSNLDEAITEASKADLFIVIGSSLQVSPANLLPEYSLARQGDLI
                     IINYMETPLDYKAALVINQDVGLFLFEVCKHLGIW"
     gene            complement(5794..6867)
                     /locus_tag="PKJ95_00755"
     CDS             complement(5794..6867)
                     /locus_tag="PKJ95_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNR64801.1"
                     /translation="MIIGILGMPLTGKTTIFNLITSQHKETDAFSSRKTKNIGVAKIV
                     DERVDILSTIYQPKKTTYASIEMIDIPGISSDMTGKTKQEIFTQIYKADALLIVIRTF
                     KDSKIPGIVDPLYQAESLIYEILLHDLEMVGNRITRLSESKRKITNQEKFEKQILERC
                     QVHLESDQLLKTQLLSEEELKILSGFNFFTLKPIIFALNVDENEINDQKITNYKNFDK
                     FIQEHQMASIAICGKLEMEINDLAEEEKKIFLEEIGFKKTGIERLSQVLYDHLGLISF
                     FTVGKDEVKAWTIYRSTTAVKAAGKIHSDIERGFIRAEVVNFIDFMQYKSMPKIKEKG
                     LLKIEGKGYLIEDGDIVNFRFNV"
     gene            complement(6916..7332)
                     /gene="nikR"
                     /locus_tag="PKJ95_00760"
     CDS             complement(6916..7332)
                     /gene="nikR"
                     /locus_tag="PKJ95_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNR64802.1"
                     /translation="MSHLVRFSISLEEALLQKFDHHINEQKYKNRSEAIRDLIREKLV
                     KKEWAENEKVAGTITLVYSHHKRGLVQKLTSLQHDYHHMIISTQHIHLDHDNCMEVII
                     THGKARSLEQLADKLKTVKGIKYGNLAMATYGRELI"
BASE COUNT         2216 a         1384 c         1253 g         2631 t
ORIGIN      
        1 caatacagcc ttgcgttgta taggagatgc cagaagcccc atgcttatta tgatactgac
       61 ttctctttta aatattgtcc tggacccctt cttaatgttt gatattattc caggcacttc
      121 tattacagga atgggattgg gcattaaagg agcagccatt gctacagtaa tatcaactgt
      181 tattgccttt tctattggtc tttttattct cttatcagga aaaagtttta taaaaatatc
      241 tttttctgga ttatttaaac tgagtaaaga gaatgctaaa aaactaataa taattggact
      301 tcctacgggt ttagagatgt tttttcgaca gggcagtcaa ttcctattat taaaatttgt
      361 ttcattttat ggcacactgg ccctggctac ctttggtata ggaatgaaac tgttcaatct
      421 ggtctttctt cccttactgg gcttgtcaat gggcggtagc gcggttgcag ggcaaaacct
      481 tggtgcaaac catattgaaa gaacacgcca gactgcaatc tggacttcat tactgggaat
      541 tattataacc ggtatagttt cctctcttgc cctgcttttc cctgaggcaa tacttaagat
      601 atttattaac cagaaagaag taattgaaat aggtagaaag atgatttata ttctttcccc
      661 cagttttatt gctgtagcta tctctatggg tttaaacacc gtatttgccg gttcaggcta
      721 caatttacct tatttgattt cgggaattgt ctcacgctgg ttttttcaaa tccccttttc
      781 cgcactgata atatatattt ttcatctacc cattacctac atctgggtgg gattttttat
      841 agcagaattt gtagaattac tggttattta caccttctat cacagaggga gatggaagac
      901 caccagggta taaatcaacg aggttggagg cctatgtttt ccagagtctt ctcatccata
      961 cggtaaataa cccagtctgt tagaggatag gcgcccatct tttcgtaaaa ttcccgggca
     1021 ggattccagt ccaataccac ccattccatc cttccacatt ttctttctct ggccaaacgt
     1081 acacattcca gaaacaatgc ttttccaacc cctttacctc tgaatttttc tctgacaaac
     1141 aaatcttcga tataaagacc aggtttgcct aaaaaagttg aataattatg aaagaataga
     1201 accataccta ccggttcatt ctcccaataa gcaatttctg cttcagcata tgcttttggt
     1261 ccaaatagtg tttccctgat ggcctcttca ctattgatga ctttatcggc taatttttca
     1321 aaaacggcta actcttttat cagagaaagc aaaattgagg tatcctgttc catagccggt
     1381 ctgatagtta attcttgtgg gtttaccttt tcaaaactgt tcaaatctat cttcctcctt
     1441 ccttaaaata gaacgatatt tcataagtta taaaataacc taaaatcaat cttttggcaa
     1501 taataaagat ttttttgatg aaagcattgt ccattctcgt attgcctttg tttttttagt
     1561 cattttacct ttcattgcca caaactatta aaaatagtaa aatatccaat atatccaata
     1621 aataaaaata taaaaattta tctggtagag aaaggaggga atcttattat gctggaagaa
     1681 tttaaaaaat ttattatgcg tggaaatgtg atagacatgt cggtaggtat tgtggtagga
     1741 gctgcctttg gagctattgt cagttctttt gtaaatgata ttttaatgcc acctataggg
     1801 ctcttgctgg gaggatttga tttcagtaat ctgtttattc tactaaaaca gggaacaact
     1861 cctggcccct atcttactct ggctcaggca caggaaaccg gtgcggttac cttaaattat
     1921 ggggtcttta tgaatgcaat tataagcttt cttattatag ccctggctat ctttttggta
     1981 attcgtcaaa taaaccgcct aaaaaaggca gaagaggctc caccaccctc aactaaggaa
     2041 tgcccttttt gttttacagt gatacctatt aaagccaaac gttgcccaaa ttgcacttcg
     2101 gaactggccc aatagaaaag cttattctta acttataagg caaagaaatt aatatcacag
     2161 gatattatat tctagaccaa actggtcgat tagtcgatta tttgtatttt tgtgacaaag
     2221 agatataata ttgtaaatag atggtgcagt atcctagtta atgccctgtt cctgaaggcg
     2281 ggcctaaaaa tccgtcaagg gcacacctat gaagttcctg gtgttggctg ctaacgccca
     2341 gttgggagct aatgctggga gttaagggtc aggggcgatc cgcaatggca tgcgggcgtt
     2401 gacccctttc ccgtggaggc ttctatgcat tagtaaagca taaaagcgaa cctgcatctg
     2461 ttgcaagatg ggtgcagtgt agcctgcttt gcgtggttca gggggaagca taaaaaaact
     2521 aggcaagcat tgaggtaccc gatgctatta ctatagcgga aacttgggct gctaaagaag
     2581 ctagggaaca gtgcgcattt gaaggaaaac ttctaggctg tcggcattca gccgagtatg
     2641 ccagggatta aagtgtggcc tgagtggtaa tccagtccta taccgggcga ccatatagct
     2701 ggaatatgaa agggaaaccg cctgtctggt aacagcaggt tattccaggg gaaaacctac
     2761 tggacctaag ccgcaacatt gacctggcat attaccatct atatatgcca aaatttattt
     2821 tattattatt tattcttcag aggtacatag cagcaaacct taaggtgatg gccatattaa
     2881 agaattttaa agaaagtatc tgtgtcacag tcataacctt tattctgact attatagtgg
     2941 ctttaatttc ttattcctgg atggaaacaa tatcattctt cccggctgtt cttattctga
     3001 ttattattat ctctatcggt gtcttaagtg atatggtcgc gttcgcagca cctgccgctg
     3061 aagtagaatc ctttcatgct aaagcagcaa aaaaagtttt tggtgccaag aagggattat
     3121 ttttagttaa aaatgcccac cgtgttgcta attttatggg tgatatagtt ggcgatatct
     3181 gtggaatact gagtggttct ctggggacta ttatagtgat taagacagcg agcaactggc
     3241 aggctcctaa aagctggata gatttattgg tgctcagtct tatagctgca attactgtag
     3301 gagggaaatc ttttttaaag agttacgctc ttcgtcaggc taatgaaata atcttatttg
     3361 caggaaaaat cctggccagt ccaacccttt taaaaaatat ttttaaaaag attaaaaaca
     3421 attcttaaaa aaagcatttt ttagttattc taataatcta tcttttagct tcaagtttgc
     3481 cttgtctaat attggatata aaaactttgt tcatagcgga agttggaaac ttgccctgtt
     3541 taaaaaatca aataagtttg tacaacagaa acaattctta taaattcaaa aaccctgaac
     3601 cagaggggtg gtcagggttt ttgaatttgt ttaaattctg cgatttatct gaactgatta
     3661 tgtgaataat cagctagaag tctatgtatt attttttctt tttttcagtt tctttggttt
     3721 tctttccaca ggctttagcc attttgacac ctcttttcga tagtcatatt taatgaaaaa
     3781 tactcagata ctgacttatt atcagttaaa atcttagtta atatctctaa ataacttata
     3841 cctttaaatg aatattttgt caatatattt tcatggtaaa attaaaaaag atttaggatg
     3901 gatgctctta tcttcccagt taattttgcc gcgttctttc attatctcta aggttcggta
     3961 aatgtctgcc agagaatact tgttgggcat agtatatacc aattcctgag cagtaacctg
     4021 ttccttctcc tgcaataaat catgaagata cataactatt aattcactga tcttctcgta
     4081 tttatcctct tcatccggtt gcaaggatgg cataaaagca ctgttcttat ttgagggtat
     4141 ctctttttgt ttttccagct ttttctcttc tttttggtat gatatctgat taatttccgt
     4201 ctgtctttct tctaaatgct gaatctggct tttcaattct ttaaattcat caagacttat
     4261 ccggaaatca agagaggtcc catctacatt cttgccttta ttgatattat ctttcatatc
     4321 aaaataggca gtttcataaa tatattgcga aaggttctcc aggtcggtta cattttgttt
     4381 cattctaatt atctgagatt caatcttttt taaaaccctt aaagaccata tataaaaaat
     4441 aatagccaaa caaaaggcca gaattattaa aataatggct aataaaggtg ctgtctgctc
     4501 aaatatccta atattttcca ttttttgctc cctttctaaa aatgaaaaaa atattatatt
     4561 tcttcgcttt cctttggaat ctgcttcaat aaatctttat cctgcttttc atgataatct
     4621 gttctcattt ctgatttttg tgcaacatcc tcttggtttg atgatactac ctctttttct
     4681 ttctcggcaa taatgagatt ttggggatga gtaggttttg gtaaaatcag ttccttgtca
     4741 tacaaaattt tgggtatcct ttctaaacgt atagcactta tctctaaccc cttggacatt
     4801 tcagtaatca ttagtgaaaa ttcagtataa agccgataga atagtatagc aaaccagatg
     4861 gtagataagc agataaaaat agacaggaag gagagtgcaa tagtgattaa ttctgaataa
     4921 ttcataaaat tacctctttc aataattacc agatacctaa atgtttacaa acttcaaaaa
     4981 gaaaaaggcc tacatcctgg tttattacaa gggcagcctt atagtctaaa ggagtctcca
     5041 tataattaat aattatcaaa tccccttgcc gtgctaagct gtattcaggc agtaagtttg
     5101 ccggggaaac ctgcaatgag gagcctatta ctataaacaa atcagcctta cttgcctcag
     5161 taatagcttc atctaaattt gataacatct caccaaataa gactacatcc ggttttaaaa
     5221 taccaccgca attacaatag ggaatttgct gaatattttc tattattttt tctattaaca
     5281 gctgagaaga tatttttgct ccacatctgt tacagatagc ctgggacaaa ttgccatgga
     5341 tttccaaaac attttgtgaa cctgcttttt ggtgtaaatt atcaatattc tgggttatta
     5401 ttttctgtaa atagttcgat tgctccagtc gaacaagtgc ataatgagcc tgattgggaa
     5461 ctacattctt caactcctct attctgggac gataaaagtg ataaaatcgt gccgggtttt
     5521 cccgaaaagc gtcgatactg gtaacttcta taggatttat cttagaccag ataccttttc
     5581 ccggagttcg aaaatcaggt attccagaag cagtgcttac cccggctccg gtcaaacaaa
     5641 cagtatactc ggatttctgt aataactcac ttactttttt tatttcctgt tggtttgttg
     5701 cggtcattgc tattattgga gatatttaaa tctctaaaat cctttcttaa atatgcaata
     5761 ctctaatatg tattattcca caaaattatt tttttagaca ttaaatctaa aatttacaat
     5821 atcaccatct tcaatgaggt aacctttccc ttcaatcttc aataatcctt tttcttttat
     5881 ttttggcatt gatttatatt gcataaaatc tatgaaattt actacttctg cccttataaa
     5941 acctctctct atatcgctat gtattttacc agcagctttg actgcagtag tacttctata
     6001 aatagtccag gcttttactt catctttacc cacagtaaag aacgatatca aacctaggtg
     6061 atcatagaga acttgtgata atcgttctat ccccgttttt ttaaagccaa tctcttccag
     6121 gaatatcttt ttttcttctt cagcaagatc gttgatttcc atctctaatt ttccacaaat
     6181 agcaatactt gccatctgat gctcctggat aaatttatca aaatttttat aatttgtaat
     6241 tttctgatca tttatttcat tttcatcaac atttagtgca aatattatcg gttttaaggt
     6301 aaaaaaatta aatccgctca aaatttttaa ttcttcttca cttaggagtt gagtttttaa
     6361 caattggtct gattccagat gtacctgaca tctttctaaa atctgttttt caaatttttc
     6421 ttgattggtt attttccttt ttgactcact taatcgggta attctgtttc ctaccatttc
     6481 taaatcatgg agcaagattt cgtatattaa actctctgct tgataaagtg gatcaacaat
     6541 accaggaatc ttactatctt tgaaagtgcg gataacaatg agtaaggcat cagctttata
     6601 tatttgagta aaaatctcct gctttgtttt accggtcata tcagaagaaa tacctggaat
     6661 atcaatcatt tctatactgg cataagttgt tttttttggt tgatatatcg tactcagaat
     6721 atcaactctc tcatcaacaa ttttggcaac accaatattt ttggtttttc gagaggagaa
     6781 tgcatctgtt tctttgtgtt ggcttgttat gaggttaaat atagtagtct ttcccgttaa
     6841 cggcatccct aaaataccta taatcattat tacacctcac caaatacctt aaattgtctt
     6901 aaacagtgat aagccttata ttaattctct tccataagtt gccatggcta agttgccata
     6961 tttaatgccc ttcaccgttt taagtttgtc agcaagctgc tctaaagaac gtgccttgcc
     7021 atgggtaata atcacttcca tacaattatc atgatcaaga tgtatatgct gggtagaaat
     7081 tatcatatga tggtaatcat gttgcaagga agttaatttt tggactaatc ctcttttgtg
     7141 gtggctgtat accaaagtta tagtgccagc aactttttcg ttttctgccc attctttctt
     7201 aaccagtttt tctctgatta aatctctgat tgcttcagac cgatttttat atttttgttc
     7261 attaatatga tggtcaaact tttgaagtaa tgcttcctct aaagagatac tgaaccgaac
     7321 taaatgagac ataatttgag caatccttct tttaaatatt ataaagattc tgttaaaaca
     7381 gccattggta taaaattttt agattctagt ttttagttta aatcattgca agttgcagta
     7441 ctaccatcct aaaacttata acaacttgca atattatcac aatg
//
LOCUS       DAOQWT010000015         7054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_11049_length_7054_cov_3.271610, whole genome shotgun sequence.
ACCESSION   DAOQWT010000015 DAOQWT010000000
VERSION     DAOQWT010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11049_length_7054_cov_3.271610"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /locus_tag="PKJ95_00765"
     CDS             <1..1846
                     /locus_tag="PKJ95_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNR64803.1"
                     /translation="KDPRHRPIIQDDIITIRQGRFVIPVKQQEKGKFPGVIHDKSESG
                     LTVFVEPLVVVELNNELRELFQEEKKEEYKILQRLTALVGASGEDILSNFKYLGELDL
                     INAKAEISRKMNANRPNINIKGIINLIQARHPLLKNEVVPIDIELGKKFDTLIITGPN
                     TGGKTVTLKTVGLLHLMAQSGLHIPAAIDSEIVVFGQIFADIGDEQSIEQNLSTFSSH
                     MKNIISILNAADKHSLVLIDELGAGTDPSEGSALAMAILDFLRRRGARILSTTHHDSL
                     KAYAYLTEGVMNARVEFDEKTLQPTYQVSIGLPGSSCAFAVAKRLGLSEHILNNAEGY
                     LEKEKIDLENVIKRIEKDRIHIADNLKNIAREELQIKKLKAELEEKIDSVEKERIKIR
                     LEAYLEAEKLIHSTQAKAKGIINSLKKKKDTREKYSEEVSALEDLEKEIKKQVEIYDR
                     TSEKVDDFKEGENIIIKSLHKEGIILEKNGRKGQYMVQVGNIKLRVPADDIQKVCNDD
                     SKKGEKENNLKKQAVLLQENRIEKKAHFKNEIDIRHMNAFDASIRLEKYLDDALLLNI
                     SPVYIIHGKGKGILRKTATQLLERLKYIKDYRYGETHEGGDGVTVVYF"
     gene            complement(1856..4021)
                     /locus_tag="PKJ95_00770"
     CDS             complement(1856..4021)
                     /locus_tag="PKJ95_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HNR64804.1"
                     /translation="MKSNKKNKKSHNSSPFFPKVFSLSLFLFILSFVLSFTLLISLSS
                     QKMGEIVQSSNFISPVTSKVYDINGKLITEFFQENRTPISLSQIPKNLINAIIAIEDS
                     NFYEHHGISFRGIARAMFENVKESGKIFQGQGGSTITQQLAVNTFLTREESLSRKIKD
                     ALLALQIERTFTKDEILEMYLNVIYFGHGAYGIVSAANLYFGKQVSELSLAEAALIAG
                     IPRRPYFYSPFINLEAALKRKNIILKRMFDLGYITEEEYLRGREEEITLNHNRDSLET
                     APHFSSYIRTILLDKYGVNMVFKGGLKIYTTLDLDMQKKAQEAFLKSGREGAVIALEP
                     GTGYIKAMVGGKNYEESEFNRATQAYRQPGSAFKPFVYLTAIEKGISPSLVIEDSPIV
                     YENGWAPTNYEDKFKGPVTLREAFEDSINVVGVKLLERVGVRDVIKNAQKAGIKSTLR
                     ADLSLALGTSEVTPLEMASAYSTIANMGTYIEPTAIIKIEDYSGRILEQNQIVKKDVF
                     SEDVCYTLIKLMEGVLIRGTATNARLERPAAGKTGTTNEFTDAWFVGFTPDLVCAVYI
                     GNDDRKPIGNRMTGAVVAAPIWHDFMVSALQDKPINDFPSPNNVKEIQVCAKTGLLPG
                     NKCAKTVAVTFLSGTEPTQICYESTGILPEYIVETKPPKNEVPVISVEKRFPLLDDLE
                     TLKKEEPDHQIAQPKESKKETLQSLVEELRKKLEQRDKD"
     assembly_gap    4227..4236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4237..4633
                     /locus_tag="PKJ95_00775"
     CDS             <4237..4633
                     /locus_tag="PKJ95_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HNR64805.1"
                     /translation="SYGPVQIRVAEALSVLPFIDPAAIGGLFLGCILANLWGGLGITD
                     VVGGSLSTLLAAVLTYKMKKPILAPCPPVIVNSLGISLYLHILFKLPYWLTVSYIFLG
                     QLISCYLFGYPLLLVLLKREIWIYTDKRE"
     gene            4727..5947
                     /gene="tyrS"
                     /locus_tag="PKJ95_00780"
     CDS             4727..5947
                     /gene="tyrS"
                     /locus_tag="PKJ95_00780"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNR64806.1"
                     /translation="MDLSEEMKLIKRGTKEIISEEELEQKLINSKKNKKPLVIKQGFD
                     PSAPDIHLGHTVGLRKLRHFQDLGHEVYFLIGDFTGMIGDPSGRSVTRKQLTPDEVKK
                     NAETYKEQVFKILDPEKTIVEFNSHWLGKLSFVEVIKLCSKYTVARMLERDDFANRFR
                     EQKPIGVHEFLYPLMQAYDSIALKADVELGGTDQKFNLLVGRDLQREYNQEPQVIITL
                     PLIEGTDGVEKMSKSLGNYIGINENPFDMYGKTMSIPDKLILTYFELLTDIPYPDLEK
                     IEQDLKENKTTPLTVKKRLAKEIVKIYHGYHQANEAEKNFQKVFQEKKIPENIQNLLL
                     PRHILKNNKIWIVKLIKQTNLLSSTGEILRIIEQGGVKINNKKVEETNIDLEIKEGDI
                     LRVGKLYFYRIKIV"
     gene            6340..>7054
                     /locus_tag="PKJ95_00785"
     rRNA            6340..>7054
                     /locus_tag="PKJ95_00785"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2276 a         1205 c         1476 g         2087 t
ORIGIN      
        1 aaaggaccct cgccaccgtc ccattattca ggatgatatt ataactattc gacaagggag
       61 gtttgttata cctgtcaaac aacaggaaaa aggtaaattt cccggagtga tacatgataa
      121 atctgaaagt ggtcttacag tttttgttga gccgctggta gtggtagaat tgaataatga
      181 gctacgggag ttgtttcagg aagaaaaaaa ggaagaatat aaaatactgc aaagattaac
      241 tgctttagta ggcgcaagcg gagaggacat attatctaat tttaaatatc taggtgaatt
      301 agatcttata aatgccaagg cagagataag tagaaaaatg aacgctaaca gaccaaacat
      361 aaatattaag ggcatcataa atctaattca ggccagacat cctcttttaa aaaacgaggt
      421 tgtccctata gatattgaat taggtaaaaa atttgatacc ttaataatta ccggtcctaa
      481 taccggtgga aaaacagtaa cccttaaaac ggttggctta ttacacctga tggctcagtc
      541 cggtttgcat attcccgctg caatagatag tgagatagtt gtctttggac aaatatttgc
      601 cgatattggt gatgagcaga gtatagaaca aaatttgagc accttttctt ctcatatgaa
      661 gaatattata tctatactga atgctgcaga taagcattct ttagtgttaa tagatgaatt
      721 aggtgctgga actgaccctt cagagggctc agctttagca atggcaatcc tcgatttttt
      781 aagaagaaga ggggccagga ttttgagtac tacacaccat gatagtctca aagcctacgc
      841 ctatcttact gagggagtga tgaatgccag agtagagttt gatgaaaaaa cccttcaacc
      901 gacttatcag gtttctatcg gtcttccagg aagtagttgt gcttttgctg ttgccaaaag
      961 gttgggatta tcggaacaca ttttgaataa tgcagaggga tatctggaaa aggaaaaaat
     1021 agacctggaa aatgtaatta aaagaattga aaaagatagg attcacattg ccgacaattt
     1081 aaaaaatatt gcccgggaag aactacagat taaaaaatta aaggcagaat tggaagaaaa
     1141 gattgattct gtggagaaag aaagaataaa aataaggtta gaagcatacc tggaagcaga
     1201 gaagttgata cattcaactc aggcaaaagc aaagggcata ataaattctc taaaaaagaa
     1261 aaaagacact cgggaaaaat attctgaaga ggtcagtgct ctggaagatt tggagaaaga
     1321 gattaaaaag caggttgaaa tatacgaccg aacatcagag aaggtggatg attttaaaga
     1381 aggggagaat attatcatca agagtctgca taaagagggt attatcctgg aaaagaatgg
     1441 tagaaaaggt caatatatgg tgcaggttgg gaatataaaa ctcagagttc ctgctgatga
     1501 tatacaaaaa gtatgtaatg atgattctaa aaagggagaa aaagaaaata atcttaaaaa
     1561 acaagccgtt ttattacagg aaaatagaat agagaaaaaa gcccatttta aaaatgaaat
     1621 tgatattcgt catatgaatg cctttgatgc cagtattaga ttggaaaaat atttagatga
     1681 tgctttactg ctaaatattt caccggtata tattattcat ggtaagggaa aaggaatttt
     1741 aagaaaaaca gccacccagc ttctggagag gcttaaatat ataaaagatt atcgttatgg
     1801 tgaaactcat gaaggtgggg atggggtaac ggtagtttat ttttagaata gattactaat
     1861 ctttatccct ttgttccagc ttctttctta attcttctac caatgactgt agagtctctt
     1921 ttttggattc cttaggttga gcaatttgat ggtctggctc ttcttttttc agggtttcta
     1981 aatcatcaag caagggaaat cttttttcca cagaaataac agggacttca tttttgggtg
     2041 gtttggtttc aacgatgtat tcaggtagta tgccagtgct ctcgtaacag atctgggtgg
     2101 gctcggtacc ggaaagaaat gtcacggcaa cagtttttgc acatttattg ccaggtagaa
     2161 gcccggtttt ggcacatacc tgtatttctt taacattatt tggactggga aaatcattta
     2221 tcggtttatc ctgcaatgca ctaaccataa aatcatgcca gataggggca gccaccaccg
     2281 ccccggtcat tctattgcct ataggttttc ggtcatcatt gccaatgtaa acggcacata
     2341 ctaaatctgg cgtaaagcca acaaaccagg catcggtaaa ttcattggta gtacctgttt
     2401 ttcctgcggc aggtctttcc agcctggcat tggtagcagt gccccttatc aatactcctt
     2461 ccattaattt aatcagggta taacaaacgt cttcagaaaa aacatctttc ttaacaattt
     2521 gattttgttc cagtatccta ccactatagt cctcaatctt gattatggca gttggttcaa
     2581 tataggtgcc catattagcg atagtggaat aggcagaagc catttctaaa ggggtcacct
     2641 ctgaagtgcc gagagccaaa gagagatctg ctctcagggt gctttttatt cccgcttttt
     2701 gagcattttt tatcacatcc cttaccccga cccgttccaa tagctttact cctactacat
     2761 ttatagaatc ctcgaaagcc tctctcaaag taacaggacc tttgaattta tcttcataat
     2821 tggtaggtgc ccaaccattt tcataaacaa taggggaatc ttctataacc aaactgggtg
     2881 aaatgccttt ttctatagcg gtgagataga caaaaggttt aaaagcagaa ccaggttgcc
     2941 tataagcctg ggtagctctg ttaaattcac tttcttcgta attcttcccg ccaaccattg
     3001 ccttgatata gccagtacca ggctcaaggg caatgacggc tccctctctg ccgcttttta
     3061 agaaggcttc ttgggctttt ttttgcatat cgaggtccag ggtggtataa atctttagtc
     3121 ctcccttgaa taccatatta accccatact tgtctaagag tattgttctg atataactgg
     3181 aaaaatgggg tgctgtttcc agagaatccc tgttatgatt cagtgtgatt tcttcttctc
     3241 tgcctcgaag atattcctct tctgtaatat atcccaggtc aaacattctt tttaagatga
     3301 tattttttct ctttagagct gcctctaaat ttataaaggg agaataaaaa tatggccttc
     3361 ggggaatacc ggcaatgaga gctgcctccg caagggagag ttctgaaacc tgtttgccaa
     3421 aatagagatt tgctgcagag actatcccat aggctccatg accaaaatag ataacattga
     3481 gatacatttc taaaatctca tctttagtga aagttctttc aatctgtagg gcgagcaggg
     3541 catcttttat tttacgagac aaagattctt ctctggttaa aaatgtattt accgccaact
     3601 gttgggtgat tgtactcccg ccctgtccct gaaatatttt gccactctct ttaacatttt
     3661 caaacattgc tctggcaatt ccacgaaaac taattccatg gtgttcataa aaattactgt
     3721 cttctatggc aataatggca ttaataagat tttttggaat ttgagacaag gagataggtg
     3781 tacgattttc ttggaaaaac tcggtaatca gttttccatt gatatcatat accttgctgg
     3841 taactggact tataaaattg ctgctctgca ctatttcacc cattttctgg ctggaaagtg
     3901 aaattaaaag ggtgaaagag agcacaaagg ataaaataaa taaaaaaagg gaaagggaaa
     3961 atacctttgg aaaaaatgga ctactattat gtgatttttt atttttttta ttagatttca
     4021 ttatattatc ctgatttttt ataatcccaa tttataataa aattcttccc attacctacc
     4081 gggctaacaa taatattatt tgatgtcagc ctctttaatt atattgtatt ataatctgtc
     4141 aatctttatt tacttgcaaa gaaatatgtt ataattaaaa aagctttaaa gcaaatttaa
     4201 aggattataa ttgtcaaaac catattnnnn nnnnnncagc tatggtcccg tgcaaattag
     4261 agttgctgaa gcattgtctg tgttaccatt tattgacccg gcagcgattg gcggattatt
     4321 tctgggttgt attctggcaa atttgtgggg agggcttggt attactgatg ttgtgggggg
     4381 aagtttaagc actttgttag ccgccgtctt aacctataag atgaaaaaac ctatacttgc
     4441 tccttgtcca ccggtaatag ttaattctct ggggattagt ctttatttgc atattttatt
     4501 caaactaccc tactggttga cggtcagtta tattttttta ggtcagctaa tttcatgcta
     4561 cctgtttggc tatcccctgt tactggtttt attaaaaaga gaaatatgga tttataccga
     4621 taaaagagaa taagaatttg aggcattcgt atctgaaaaa tcatacctct tgcctattta
     4681 tcagatttta attatttgta gaaggaagga gtataaatta cttaaaatgg acttatctga
     4741 agaaatgaag ttgatcaaaa gaggaacaaa ggagattatt tctgaagaag aattagaaca
     4801 aaaattaatt aactccaaaa aaaataaaaa gccattagtt atcaaacagg gatttgatcc
     4861 tagtgccccg gatatacatt tggggcatac agttggattg agaaaactgc ggcactttca
     4921 agacctggga catgaggttt attttctgat tggcgatttt accggaatga ttggtgaccc
     4981 aagcggccgt tcagttacca gaaaacagct aacaccggat gaggtgaaaa agaatgctga
     5041 aacttataag gaacaagtat ttaagatact ggacccggaa aaaacaatag tagagtttaa
     5101 tagccattgg ttaggcaaat tatcctttgt ggaggtgata aaattatgct ctaaatatac
     5161 cgttgctcgc atgttggaaa gagatgattt tgctaatagg ttccgggaac aaaaacctat
     5221 aggtgttcat gaatttttat atccccttat gcaagcatat gattctattg cacttaaggc
     5281 tgatgtggaa ttagggggca ctgatcaaaa atttaacctg ttagtgggca gagacctgca
     5341 aagagaatat aaccaggagc cgcaagttat cataacctta ccccttattg aggggactga
     5401 tggtgttgaa aaaatgagta aaagtctggg gaattatatc ggaattaatg aaaacccctt
     5461 tgatatgtat ggcaaaacaa tgtctatacc agataaattg attctaacct attttgagct
     5521 attaacagat attccctatc ctgatttgga aaaaattgag caggatttaa aagaaaacaa
     5581 aactactcct ttgactgtaa agaaaagatt ggcaaaagag atagtaaaaa tataccacgg
     5641 gtatcatcaa gccaatgagg cagagaaaaa ttttcaaaaa gtgtttcagg agaaaaaaat
     5701 tcctgaaaat attcaaaatt tattgcttcc caggcatatt ttaaaaaata ataaaatatg
     5761 gattgtaaaa cttataaaac agaccaattt gttgagtagt actggagaaa ttttacggat
     5821 tattgagcaa ggtggtgtta aaatcaacaa taaaaaggta gaggaaacta atattgattt
     5881 agaaataaaa gagggagata ttttaagagt aggcaaatta tatttttatc gtatcaaaat
     5941 agtatagtta tttttgaatc atcagtttcc ggttcatttg aaaaaaatat tattatataa
     6001 tataattaat ttagagtttt taaacattga atttttatat ttagaacatc tttataatat
     6061 ttataatatt agttaaatta tttttaaatt ttcttgacat ctcccccttt ctctgttact
     6121 atcttatttg cccttccgga aggagactat cctaagcttc tcaccgagag ggtaaacgta
     6181 ctttgataat gaaatatagc acccagccag atgctttgat caaaagataa ccaaagggct
     6241 atccccaatt tttgaggata acccttatca ttcggtgtca attttggagg gagaggagag
     6301 ggagagattt cggtctttct ctttttaatc cctttaaaga aaacggagag tttgatcctg
     6361 gctcaggacg aacgctggcg gcgtgcctaa cacatgcaag tctgacggga aagtctcctt
     6421 cgggagaccg gtacagtggc ggacgggtga gtaacgcgtg ggaatctacc cggtagtggg
     6481 gaataaccct ccgaaagggg agctaatacc gcataagccc caaggggtaa agatggcctc
     6541 ttttctaatg ctatcgcttc cggatgagcc cgcgtcctat tagctagttg gtgaggtaat
     6601 ggcttaccaa ggctacgatg ggtagccggt ctgagaggat gaacggccac actgggactg
     6661 agatacggcc cagactccta cgggaggcag cagtggggaa ttttgcgcaa tgggggaaac
     6721 cctgacgcag caacgccgcg tggatgacga aggccttcgg gtcgtaaagt cctgttctgg
     6781 gggaagaaca agtagagggc caatacccct ttaccctgac ggtaccccag cagaaagctc
     6841 cggccaatta tgtgccagca gccgcggtaa tacataagga gcaagcgttg tccggaatca
     6901 ttgggcgtaa agcgctcgta ggcggtcctg aaagtcagct gtgaaatcac caggctcaac
     6961 ctggtagggc cagctgaaac tccaggactt gagggtagaa gaggaaagcg gaattcccgg
     7021 tgtagcggtg aaatgcgtag atatcgggag gaac
//
LOCUS       DAOQWT010000016         6969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_11218_length_6969_cov_3.138704, whole genome shotgun sequence.
ACCESSION   DAOQWT010000016 DAOQWT010000000
VERSION     DAOQWT010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11218_length_6969_cov_3.138704"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2594)
                     /locus_tag="PKJ95_00790"
     CDS             complement(<1..2594)
                     /locus_tag="PKJ95_00790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR64807.1"
                     /translation="MKTILLVVESLEPGNLHRGLYNGFLQNSLIEGKEINIIPLIFPR
                     DVNFKWTIYDEMILHTIKKFKIDFFVSIWGEQISIETLKELQRKGIKTITWQIDDPFM
                     LTENYGIETRKKLPFYDVIYTTNKASIENHYKNMGLSNKVNFLPFGYDPMFHKNLNLE
                     KQYNVSFVGSNFPTRQKLYLSKFSCNVNTFGILPNSRITHYKMVEIVNQSKINLNFAD
                     QPVNGVRCLKNRVMEVLGCEQFLLTEIFPELEEMFKPGEDLDCFSSAEEMNSKIDFYL
                     KNTSAREKIAKSGAEKIRKYQYKNITSQIINNIPVQKKENNIKKITKILIVSHHFDLP
                     VDKIPTGGVQRHIQAITKEFVKRGYEVEWCYLPEAKRELSNFNPDIVIANDFSSFVND
                     CPVPQIVIFHGYEGNIPPLQQIIKRRKEVESKCNASICVGEYLKKWYNQNPDKTIWGG
                     VEEVSIVNPPRRNNILYLGRLDPDQSAEKIFNYLGEAHKKEGIKFHLSVCGSGKLEKK
                     IKEIAKKYKLNATFYGFVKNPDEYIKKADIVICSGYLSILESFINKRPVLTFYENELK
                     KDYLSLMPVNFLPNNVSLSKVFNSISPEHIEANYQFALKNTWSKVADDYEELFKKCLP
                     VEREVITEKLPEGIKNCRICKSDNIKEVLNLGNLYLSGIFPAPGEEINKSPLVLVRCC
                     ECGLIQLKHTYNLEEMYGNNYGYHSNLNSSMIRHLKELALYIEEKYSISPKEIIIDIG
                     SNDATLLKQFSRGIKIGVDPTIEKFKEYYSENIQGFPDFFPSENLNKFLSGSKVKVIT
                     SIACFYDLEDPVYFAQEIAKLLSREGIWVMEQSYMPSMVKNLAYDTICHEHIEYYGLK
                     QIKDIADRA"
     gene            complement(2591..3193)
                     /locus_tag="PKJ95_00795"
     CDS             complement(2591..3193)
                     /locus_tag="PKJ95_00795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR64808.1"
                     /translation="MPVSHTYQISSIMESVILCNPKSILEVGIGFGKYGVLCYERLNL
                     WWHGLTKEDYQLKKVRIDGIEIYKHYQNPIYKFVYDSVFWGNALDFMTKFKDKSYDLI
                     LLIDVLEHFNKEDGVKLLNECKRVSKNILISTPKDIGNQSQHFGNEAECHKFQWTKEL
                     YNEFLPENKTFINNRHSIILFYGEKWEIVYNQKSFTGEQK"
     gene            complement(3196..4014)
                     /locus_tag="PKJ95_00800"
     CDS             complement(3196..4014)
                     /locus_tag="PKJ95_00800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR64809.1"
                     /translation="MSDKMKKECSIIVSNKNRIELLKQCLKSIEENTKDVNYNLHVID
                     VGSTDGSREYLQELYDSKKIYRLILEDTPSTYAESNNREMRKCKTPYIYLLNNDCLVL
                     SGWLRNAIDFAKSDKKIGHVASLVLWNENKVQSHGANILKNGNTHCCYQGLNKDDARL
                     KEIKNYSYAGLGLYRNDLLKKVDYMPEYPSKIYWSDTGYAMRVWEAGYDVRYCPDSHV
                     IHTLHPSERTCHSADVNQGRIAFMEDWGKFLEENNGFTPDYPFTGKRPYRNRSK"
     gene            complement(4011..5027)
                     /gene="gmd"
                     /locus_tag="PKJ95_00805"
     CDS             complement(4011..5027)
                     /gene="gmd"
                     /locus_tag="PKJ95_00805"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002148036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNR64810.1"
                     /translation="MSKKKALITGITGQDGSYLAEFLLLKGYEVHGIVRYSSSENTQR
                     IKHILDDITIHYGDVTDYNRINLIIGKVNPDEVYNLAAQSHVGTSFKLPVYTFQVNAQ
                     GTLNLLNAIRENACDGGKITKFYQASTSELYGNSDDICQNESRNFYPKNPYACAKLFS
                     YWITVNYRESYGMYACNGVLFNHESPRRGEKFVTRKIAKAIKEMKQNWGDHTPLKLGN
                     IYTCRDWGYAPEYVKAMWQILQQDKPEDFVIATGETHTIKEFITEAFTIAGYDVFWQG
                     EGLNEKLFDRDMDIPLVEIDPGLFRPCETKFLKGDYTKANKTFGFQPKVTFKELVKIM
                     IEGE"
     gene            complement(5028..5288)
                     /locus_tag="PKJ95_00810"
     CDS             complement(5028..5288)
                     /locus_tag="PKJ95_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64811.1"
                     /translation="MVVYNVFKFEKSSIPNSKVKHITGYCGTFGKREDAEIVRSNEEK
                     KMRDFHDEINKKIFKRDPEKLPAFEKTQREKYTIEIQECEVL"
     gene            complement(5288..5734)
                     /locus_tag="PKJ95_00815"
     CDS             complement(5288..5734)
                     /locus_tag="PKJ95_00815"
                     /inference="COORDINATES: protein motif:HMM:NF021185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HNR64812.1"
                     /translation="MSTDIAILNIEEAIISRLHEKLPEVKVIGFPDNPDKYTWTNATG
                     EILVNFQGAGFSDPIDPMQPIQIEEDEFLLNISIKGLRTHSGAYSYIERVRLALTGYS
                     IPGQPSSCLPLRPKNIRFRNQRAGIWTYGFLFKLKRKVCFDPEVND"
     gene            complement(5685..6170)
                     /locus_tag="PKJ95_00820"
     CDS             complement(5685..6170)
                     /locus_tag="PKJ95_00820"
                     /inference="COORDINATES: protein motif:HMM:NF018704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HNR64813.1"
                     /translation="MGNYCTLEEMITVVPEQTIIELSDDSGEAITHDEDNVNAAIEQA
                     GDDIDGYLRGSYELPLTTIPATIKNIALDLAVYNLWTRRPERELPEVILRKYRDAMAK
                     LKDIQRGVFTLDIAMLNEDTLNGSLGVTNKTRYSRIFNHRKMRHEYGYSNSEYRRGNY
                     F"
     gene            complement(6185..6574)
                     /locus_tag="PKJ95_00825"
     CDS             complement(6185..6574)
                     /locus_tag="PKJ95_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64814.1"
                     /translation="MSKIASGQPGVVGNLIAAADLDQEIFVGFDGNVCAAGAKAAGIS
                     MARYNEAEVAAIVKTGSWPVQVGGTISAAGVALASDEDGYAVEADTPAGTTEITGADL
                     IEINGYASGVDPSVYPITSGTVLVDFK"
     gene            complement(6590..>6969)
                     /locus_tag="PKJ95_00830"
     CDS             complement(6590..>6969)
                     /locus_tag="PKJ95_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64815.1"
                     /translation="YTQKGIISLELMAEFFDIPNIYLGASRYVNPDNNEVFDKIWGNV
                     VIVYYQAEKFVRPAKEVYAPEGLQELFDVEEPSFGFTVRREGNPYVFEEQKSAKVRHI
                     NYTDNFKAYMTWPDAGYIINDAV"
BASE COUNT         2053 a         1377 c         1010 g         2529 t
ORIGIN      
        1 gccctatctg ctatatcctt gatttgcttt aatccataat actcaatatg ctcatgacat
       61 atcgtatcgt aagcaaggtt cttgaccata gaaggcatat aactttgttc cattacccat
      121 attccttcac gggaaagtaa ttttgctatc tcttgagcaa aataaacagg atcttctaaa
      181 tcgtaaaaac aggcaataga ggttattact tttactttac taccagagag aaatttatta
      241 agattttcag atggaaagaa atcaggaaaa ccctgtatat tttctgagta atactcttta
      301 aatttctcta tagtaggatc aacccctatt ttaattcccc tactaaattg ctttaataaa
      361 gtggcatcat tacttcctat gtcaataatt atttctttag gggaaataga atacttttct
      421 tctatataca gagcaagttc ttttaaatga cgtatcattg aactgtttaa gtttgaatga
      481 tagccgtaat tatttccata catttcttca aggttatagg tatgtttcag ttgaataagg
      541 ccacactcac aacaccttac aagaaccaga ggagatttat ttatttcttc tccgggggca
      601 ggaaatatac cggataaata aagattacca agatttaaaa cctctttaat gttatctgat
      661 ttacatatcc tgcaattctt tattccttcc ggtagttttt cggtaataac ttctctttct
      721 accggtaaac attttttaaa caattcctca taatcatctg ctactttcga ccaggtattt
      781 tttaatgcaa actgataatt tgcttctata tgttcagggg aaatagaatt aaatacttta
      841 cttaaagata cattattagg taaaaaatta accggcatca acgaaaggta atcttttttt
      901 aattcattct cgtaaaatgt taaaaccggt cttttgttta taaaactttc aagaattgag
      961 agataaccag agcaaattac aatatctgct ttttttatgt attcatcagg attttttaca
     1021 aatccgtaaa aggtagcatt tagtttatat ttctttgcta tttcttttat cttcttttca
     1081 agtttaccag aaccacaaac cgaaagatga aattttatac cttctttttt atgagcctct
     1141 cctaaataat tgaatatctt ctctgctgat tgatcagggt ctaatctacc aagataaaga
     1201 atattgtttc ttcttggagg attaacaata gaaacttcct ctactccacc ccatattgtc
     1261 ttgtcagggt tttggttgta ccatttttta agatattcac cgacacaaat agaggcatta
     1321 catttactct cgacctcttt tctccttttt attatctgtt gtaaaggtgg gatatttccc
     1381 tcatatccgt gaaaaattac aatctgtgga acagggcaat cattaacgaa actactaaaa
     1441 tcatttgcaa taacaatatc aggattaaaa tttgataact cacgttttgc ctctggcaag
     1501 taacaccatt caacctcata tccccttttt acaaattcct tcgttattgc ctgaatatgc
     1561 ctctgaaccc ctcctgtggg tatcttatcc acaggtaaat caaagtggtg agaaactatc
     1621 aatatcttag ttatcttttt tatattattt tctttttttt gaacaggaat attatttatt
     1681 atctgtgagg ttatattttt atattgatat tttcttattt tttctgcccc ggattttgct
     1741 attttttctc ttgcagaagt gttctttaag taaaaatcta ttttactatt catctcttca
     1801 gcactggaaa aacaatctaa atcctcaccg ggtttaaaca tttcttcaag ttctgggaaa
     1861 atctcagtca aaagaaattg ctcacaacca agaacttcca ttactctatt tttaagacat
     1921 cttaccccat tcaccggttg atctgcaaaa ttcaaattaa tctttgattg attgacaatt
     1981 tcaaccatct tgtaatgagt tatcctgcta ttaggtaata ttccaaaggt atttacatta
     2041 caagagaatt tagataaata caacttttga cgggtaggaa agttagaacc aacaaaggaa
     2101 acattatatt gtttctctaa attcaagttt ttatgaaaca tagggtcata tccgaaagga
     2161 aggaaattaa ctttattaga taaccccata ttcttataat ggttctcaat gcttgcctta
     2221 tttgttgtat aaattacatc ataaaagggt aacttctttc tcgtttcaat tccataattt
     2281 tctgtaagca taaaaggatc atctatctgc caggtaatag tttttatgcc ttttctctga
     2341 agttctttta aagtctctat ggatatttgc tctccccata tagaaacgaa aaaatctatc
     2401 ttaaattttt taatggtatg caatatcatt tcatcatata tagtccactt gaaatttaca
     2461 tctcttggaa aaatcagagg aataatattt atttctttac cctcaattaa actattttgt
     2521 aaaaagccat tataaagtcc cctgtgaaga ttgccgggtt ctaaactttc tacaaccaaa
     2581 agaattgttt tcatttttgt tctcctgtaa aagatttttg attatacact atttcccatt
     2641 tctcaccata gaaaaggata atagagtgtc tattatttat aaatgtttta ttctcaggta
     2701 aaaattcatt atataattcc tttgtccact gaaatttatg acattcagct tcatttccaa
     2761 aatgctgact ttggtttcct atgtcttttg gagtggatat taatatattt tttgatactc
     2821 tcttgcactc attaagaagt tttacaccat cttccttgtt gaaatgttca agtacgtcaa
     2881 taagaagaat gagatcgtaa cttttatctt taaattttgt cataaaatcc agagcgttac
     2941 cccagaaaac actatcgtaa acaaatttat atatcgggtt ttgataatgc ttgtatatct
     3001 ctattccatc tattcttact ttttttaatt ggtaatcttc tttagttaac ccatgccacc
     3061 ataaatttaa tctctcatag caaagaacgc catacttacc aaaccctatt cctacctcaa
     3121 gaatagattt tggattgcat aaaattacac tttccattat tgaagatatt tgataagtat
     3181 gagatactgg catatttatt tactcctgtt tcgataaggt cgttttcctg taaacggata
     3241 atcaggtgta aaaccattgt tttcctcaag gaatttaccc caatcttcca tgaaagcaat
     3301 tctaccttga tttacatctg ctgaatgaca agttctttct gatggatgga gggtatgtat
     3361 aacgtgactg tctggacaat atctaacatc gtagcctgct tcccatactc gcatcgcata
     3421 gcccgtgtca ctccagtata tctttgaagg atattcaggc atgtagtcaa cttttttgag
     3481 aagatcattc cggtataaac caagcccggc gtaagagtaa ttttttattt cttttaatct
     3541 cgcatcatct ttatttaatc cctgataaca acaatgagta ttaccattct ttaaaatatt
     3601 tgccccgtgt gattgaacct tgttttcatt ccataaaaca agtgatgcca catgacctat
     3661 tttcttatca ctttttgcaa agtctattgc attacgaagc catcctgaaa gaacaagaca
     3721 gtcattgttt aatagataaa tatagggggt tttacatttc ctcatttctc tgttattact
     3781 ttctgcgtaa gtagaagggg tatcttcaag gattaaacga taaattttct tactatcata
     3841 taattcctgt aaatactccc tgcttccatc agtagacccc acatctatta catgaagatt
     3901 ataatttaca tctttagtat tttcctctat ggattttaaa cactgtttta aaagttctat
     3961 tctattttta ttacttacga taatactaca ttcttttttc attttatcac tcattcccct
     4021 tctatcatta tttttacaag ttctttaaat gtcactttcg gttgaaaacc aaaagtctta
     4081 tttgctttcg tataatcacc tttaaggaat ttcgtttcac agggtcggaa taacccaggg
     4141 tctatttcca ctaaaggaat gtccatatct ctatcaaaaa gtttttcatt taacccttcg
     4201 ccctgccaga aaacatcata ccctgcaata gtaaatgctt ctgtgataaa ttcctttatt
     4261 gtatgggttt ctccggttgc aataacaaag tcctctggtt tatcctgttg aagtatctgc
     4321 cacatagctt ttacgtattc aggggcatat ccccaatctc tacaggtata gatattgcca
     4381 agttttaaag gagtatgatc gccccagttt tgcttcattt cttttatggc ttttgctatt
     4441 ttacgggtaa cgaatttctc tccacgtctt gggctttcat ggttgaatag cacaccgtta
     4501 cacgcataca taccatagga ctctctataa tttaccgtta tccaataaga gaaaagtttt
     4561 gcacaggcat aaggattttt ggggtaaaaa ttccggcttt cattctggca tatatcgtca
     4621 gaatttccgt ataactcact cgtagatgcc tgatagaatt ttgtaatctt accaccatcg
     4681 caagcatttt cccttatggc atttagtaaa ttaagagtcc cttgagcatt tacctgaaag
     4741 gtgtaaacag gtaatttaaa agaagttcct acgtgactct gagcggccag attatatact
     4801 tcatcggggt taactttccc tattataaga tttattctat tgtaatctgt tacgtcacca
     4861 tagtgaatgg ttatatcgtc aagaatatgt tttatcctct gagtgttttc tgaagaagaa
     4921 tagcgaacaa taccatgaac ctcatacccc tttaataaaa gaaattccgc aagataagaa
     4981 ccatcctgac ctgttattcc cgtaataaga gctttctttt tactcatcta taatacctca
     5041 cactcctgaa tttctatagt atatttttct ctctgtgttt tttcaaatgc cggtaatttt
     5101 tcaggatctc ttttgaatat ttttttattt atttcatcgt gaaaatctct cattttcttt
     5161 tcctcattac tacggactat ttcagcatct tctctctttc cgaaagttcc acaataacca
     5221 gttatatgtt ttactttcga gttaggaata ctactttttt caaatttaaa aacattatat
     5281 acaaccatta atcatttacc tccgggtcga aacagacttt ccttttcaat ttaaataaaa
     5341 agccataagt ccatatacct gcccgttgat ttctaaatct tatatttttt ggccttaagg
     5401 gtaaacagga agaaggttga cccggtatgc tgtaaccggt taatgcaagc cttactcttt
     5461 caatatagga ataagcccct gaatgagtcc tcagtccctt aattgaaata ttaagaagaa
     5521 attcatcttc ttctatctgt attggttgca tagggtcaat aggatctgaa aaccctgccc
     5581 cttgaaaatt tacaaggatt tcacctgtgg catttgtcca ggtgtattta tcagggttat
     5641 caggaaaacc aattactttg acttcaggta acttctcatg aagtctagaa ataattgcct
     5701 cttctatatt cagaattgct atatccgtac tcatgtcgca tctttctatg attaaatatt
     5761 cgactatatc ttgttttatt tgttactcca agtgaaccat ttaaagtatc ttcatttaac
     5821 attgcaatat ccagagtgaa aacccctctc tgaatatctt taagttttgc catggcatca
     5881 cgatattttc ttaatataac ttcaggtaac tccctttcag gtctacgagt ccagaggtta
     5941 taaactgcca aatccaatgc tatgttttta atagtagccg ggatcgtagt tagagggagt
     6001 tcgtaactcc ctcttaagta accatctata tcatctcctg cctgttcgat ggcggcattt
     6061 acattgtcct catcgtgagt tattgcctcg ccgctatcgt ctgacagttc gattatggtc
     6121 tgttccggca ctactgttat catttcttca agagtgcagt aattacccat aattcaccta
     6181 ttccttattt aaagtccaca agaacagttc cgcttgttat tggatatacc gaagggtcta
     6241 ctccactcgc atatccattt atttcaatca ggtctgcacc ggttatttca gtagtgccag
     6301 caggtgtatc tgcttcaaca gcataaccat cttcatctga tgctagagcc actcctgcgg
     6361 ctgaaattgt gcctccaacc tgaacaggcc atgatcctgt tttcactatg gcggccactt
     6421 cagcttcgtt gtatcttgcc atagaaatac ctgcggcttt tgcccctgcg gcacatacat
     6481 taccatcaaa acctacgaag atttcttgat ccaagtcggc ggcggcaatt aaattaccca
     6541 ctactccggg ctgtccactt gctatcttac tcatatgctt tattcctcct tatacagcat
     6601 cattgattat ataaccggca tcgggccagg tcatataagc tttgaagttg tcagtataat
     6661 tgatatggcg aactttagca gatttctgct cttcaaatac atagggattt ccttcacgtc
     6721 ttacagtgaa accgaaagaa ggttcttcca catcgaataa ttcctgtaat ccttcagggg
     6781 cataaacttc tttggcaggt ctgacaaatt tctctgcctg ataatatact attactacgt
     6841 tgccccatat cttatcgaat acttcattat tgtcaggatt tacatatctg cttgcaccga
     6901 gatagatgtt tggtatatcg aaaaattcag ccataagttc aagactgata attcccttct
     6961 gggtgtatt
//
LOCUS       DAOQWT010000017         6878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_11423_length_6878_cov_3.349113, whole genome shotgun sequence.
ACCESSION   DAOQWT010000017 DAOQWT010000000
VERSION     DAOQWT010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11423_length_6878_cov_3.349113"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PKJ95_00835"
     CDS             complement(<1..389)
                     /locus_tag="PKJ95_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNR64816.1"
                     /translation="MIPRKVYMDNNATTPLHPEVKKELIKAMDVFGNPSSMHQFGQAA
                     KIMMREARESVASFIGCSPEEIIFTGSGSEANNTVLSILACPSKQCSFFEGGRYKKVI
                     TSVIEHPCVMETVKCLSDRDIEVKYIDV"
     gene            complement(441..887)
                     /locus_tag="PKJ95_00840"
     CDS             complement(441..887)
                     /locus_tag="PKJ95_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNR64817.1"
                     /translation="MKLSTRSRYGARLMLKLALNYQKGPISLKDIAQQEEISEKYLSQ
                     LVIPLRASGLIYSLRGSQGGYQLSKSPSQISIKDIVQALEGNIFPVECVKNPSICSKA
                     DDCSVRDIWENLYEKIHEVLDSVSLKDLLESKELKEELVETKICEK"
     gene            complement(1089..2192)
                     /locus_tag="PKJ95_00845"
     CDS             complement(1089..2192)
                     /locus_tag="PKJ95_00845"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNR64818.1"
                     /translation="MNKGKKRRIAILTGGGDCPGINAVIRAITKKAILEHDMEIIGIK
                     DGYEGLIDNQYRTLNFDDVSGILTLGGTILGTSKKANPFQYAVNEGDGDKLKFEDLSA
                     VVLKNIEALKIECLVCIGGDGTLWISNRFHKLGVPIIGVPKTIDNDIKGTDITFGFDS
                     AVNIATEAIDRIHTTEQSHHRIMLVEVMGHKAGWIALHSGIAGGGDVILIPEIPYHSE
                     KIVDYVLQRRKTGRRFSIIAVAEGSKPVGGEVVIKRIVKESSDPIRLGGISFVLGEEL
                     GKMSGLETRSVVLGHLQRGGTPTPFDRVLATRLGSKAVDLIIEKNYGKMVSVRGDHLE
                     DFPLEEVATGPRLIPADYELIKAAREIGVCFGD"
     gene            complement(2410..2829)
                     /locus_tag="PKJ95_00850"
     CDS             complement(2410..2829)
                     /locus_tag="PKJ95_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNR64819.1"
                     /translation="MEYKKFGNKYIIRIDKGEEIVDNLKIFCEKEQITLGWITGIGAV
                     NKATIGLFKTDSKIYQTTELRGDYEITSLVGNISRMDGKVYLHLHINLSDINYNVFGG
                     HLNSAVVSGTGEFIVESIEGQVNRQFDKEAGLNLYQF"
     gene            complement(3105..4307)
                     /locus_tag="PKJ95_00855"
     CDS             complement(3105..4307)
                     /locus_tag="PKJ95_00855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR64820.1"
                     /translation="MKYQERWIALAYIYFFMLVFALTFQSIPPLLGFLVQSLGISHTQ
                     AGALMSLFGLPGILVSIPGGILADVYGPKRVGVTALLITLIGSLLVGLGKDFSLLVTG
                     RIISGIGGLTISIVAPQALSRWFASRDLGKAMGIFNTAMPLGSIFTLNVFSVLAGIYT
                     WQVPLFLTSFFCLGVLVLFYFRYPKLPDEIRRQEQPEFEEKVFTLRKTGPQVWLVAAI
                     WMMYNASTISYLTFGADYYQSVGYDVSYAGFLTSLLMVGSLLFSPLVGTLIDRVGREE
                     YFIIVGSIAIALLLYVVPRTLFNPLIIGGLIGFFAASIPSPVFALVPKYISLSKVGLG
                     YGILSTCLNIGILLGPLLVGFSYDQTQNYLVGFDLMAIFAFLTALIAFILRLIAKRIK
                     TQQPFLDG"
     gene            complement(4449..5420)
                     /locus_tag="PKJ95_00860"
     CDS             complement(4449..5420)
                     /locus_tag="PKJ95_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HNR64821.1"
                     /translation="MVLFIGYDEVLELMSMSECIDVMKDALIRLSNGEVAQYLRTATH
                     LPDGNILGFMPCYSGKSGYFGAKIITVFKTNMGSGYPSHQGMVLLFDAQYGYIKAFLD
                     ASAITKIRTAAVSAVATELLSRKDVSTLAILGAGEQARSHLEAMLLVRNFTEVRVWDV
                     NKDYSRLFVQEMKKQYDVNIFEAENAEEAVCNSDVICTVTSSKTPVLEGKWVKPGTHI
                     NAVGACSPDARELDSEIILKSKLYVDFVESTINEAGDFLIPKQEGIINNNHILGEIGD
                     LLTDSIAGRSNNIDITIFKALGLAIEDIAAAFYVYQKAKNNNRGKVI"
     gene            complement(5797..>6878)
                     /locus_tag="PKJ95_00865"
     CDS             complement(5797..>6878)
                     /locus_tag="PKJ95_00865"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HNR64822.1"
                     /translation="TTATPDAVIYYTLNGLTPSSNSTKYTAPFIVSTAKPEGEIIIVK
                     AMGTRRGYTNSAVSTRDITFKVKICTLTYNHSANGSVIGEILQAVEYGNDGKAVEALP
                     DEGYYFVKWSDGSQDNPRMDTNVNKDITVEAIFEEIAVTGITVKSQPDRLNYVEGQNL
                     KLEGLVVTLLYNNNSTEDVVFADFGEKGITASPEDGTELSITDHGEIIVITHTASGKT
                     INTVHLSIVARALTIEADSANHQITITSNYRDVVTGSTEAMIIKGGSTTSWVNLGNEL
                     QAKVITNFPCNMTCLILTPDAENDIAIVSGYREKDTRVITMDQLITVSIRDNKYTTVT
                     KSFTVEVSGSGAGINLTIGTITVLP"
BASE COUNT         2061 a         1444 c         1064 g         2309 t
ORIGIN      
        1 acatcgatat attttacctc tatatctctg tctgagaggc attttacagt ctccattaca
       61 cagggatgtt caataactga ggtaattact tttttatacc tcccaccttc aaaaaaactg
      121 cactgtttgg aaggacaggc taaaatagag aggacggtat tgtttgcttc agaccctgaa
      181 ccggtaaaaa taatctcttc aggactgcaa ccgataaaag aagctactga ctctctggct
      241 tccctcatca ttattttggc tgcctgtccg aattgatgca tactagaagg gttaccaaat
      301 acatccatag ctttgattaa ttctttcttt acctcaggat gtaagggagt ggttgcatta
      361 ttatccatat acactttacg cggaatcata tttactgttc tcctcaagac tattttatat
      421 aaaaatttat tattataata ttatttttca caaattttag tttctaccaa ttcttctttt
      481 aattctttag attctaataa atcttttaaa gaaactgaat ccagtacttc atggattttt
      541 tcgtagagat tctcccagat atcacgaaca gaacaatcgt cagctttgga acagattgat
      601 ggatttttta cacattccac aggaaaaata ttcccctcca gagcttgtac tatgtctttt
      661 atgcttattt gagaagggga cttgcttaac tgatatcctc cttgagaacc ccgtaaagaa
      721 tatattaatc cgcttgctct caaagggatg actaactgac ttaaatattt ctctgatatc
      781 tcttcttgtt gggcaatatc ctttaaagag attggtcctt tctggtaatt taaagccagt
      841 tttaacatca atctagcccc atatcgtgag cgtgtagaca gtttcatcag gattattctc
      901 ctcttaacaa tcgtttacac tactaatact attcttttag tagtaattaa taatataata
      961 aaaaaatatt tctgtcaata gtttgtcaat aattgtatag ttttactaaa cttgcctgtt
     1021 tatatctata taatcttaat aacaaacata tttttaactg attactcagt gtgtatctaa
     1081 gccaaaatct aatctccgaa acagacccct atctctctgg ctgctttgat aagctcataa
     1141 tcagcaggta tcaaacgggg tccggtagca acctcttcta aaggaaaatc ttctaaatgg
     1201 tctcctctga cgcttaccat tttaccgtaa tttttttcta taatcagatc aactgctttg
     1261 gaacccaaac gggttgccag caccctatca aaaggagtag gggttccccc tcgctgtaaa
     1321 tgtcccagta ctacagaacg agtttccagt ccgctcattt tccccagctc ttcgcccaaa
     1381 acaaaactga tacctcctaa acgaattgga tctgaacttt cttttactat ccttttaatt
     1441 accacttctc cccctactgg tttcgaacct tcagcaacag caataatact gaagcgacgt
     1501 ccggtctttc ttctttgaag aacataatct acaatcttct ccgaatgata gggaatttca
     1561 gggattaaaa ttacatcccc tccaccggca atgccagagt gaagagcaat ccaccccgct
     1621 ttatgaccca taacctcaac aagcattatg cggtgatgcg attgttctgt tgtatggata
     1681 cggtcaattg cctctgtggc aatatttaca gcagaatcaa aaccaaaggt aatgtctgta
     1741 cccttaatat cattgtctat tgtttttggt accccaataa taggaactcc cagtttatga
     1801 aaacggttag agatccaaag agttccgtca ccaccaatac ataccagaca ttcaattttc
     1861 aatgcttcaa tattttttaa taccacagcg gaaaggtctt caaacttcaa tttatcacca
     1921 tcaccttcat tgaccgcata ttggaaagga ttggcttttt tgctggttcc cagtatggtt
     1981 ccacctaaag taaggattcc agaaacatca tcaaagttca aagtgcggta ttgattatca
     2041 attaatcctt cataaccatc tttgattcca ataatttcca tatcgtgttc caggattgct
     2101 tttttagtaa tggctctgat tacggcatta atacccggac aatctcctcc gccggtaagt
     2161 atggctattc ttcttttttt acctttattc attggattat cctcttcttt ttttcttaat
     2221 atttttctac ttctcgttta ttattttgct ataagaaaat tattaactca tattactttt
     2281 tcacattatg ctccaaagat actaataact tgtttcaaga aatatttttc aattccacaa
     2341 acagtttgat ggccggtatc tctagtaaat ttttgagcta cgattatctt accccttgaa
     2401 gtacaaactt tagaattgat ataaatttag ccccgcttct ttgtcaaatt gtctattaac
     2461 ttgtccttca atagactcca caataaattc gcctgtacca cttaccaccg ctgaattaag
     2521 gtgtccacca aacacattgt aattaatgtc agataaattg atatgcaaat gtaaatatac
     2581 ttttccatcc atacgagaga tattccctac cagactggtt atctcgtaat cccctcttag
     2641 ctcggtggtt tgatatattt tgctatctgt tttaaataat cctattgtgg ctttattaac
     2701 cgctccaatt cccgttatcc aacccagtgt tatttgctct ttttcacaaa aaattttcag
     2761 gttatctaca atctcctcac ccttgtcgat ccttataata tatttattcc caaacttttt
     2821 atattccact atttttctcc ttttttcaga ttttattcta tagcactgct ggcgtatttc
     2881 tcagctccaa acttgctctt attctcagta ttatagcacg ataaccactt acctatactt
     2941 gatattgaag gtctttatcc attccaataa aataaaacaa accgcatact tctgtttaga
     3001 ttacagattc attttttata atttcacaaa tatcgggttc atactatatt ttctttctaa
     3061 taaaaaatag tatattgatt tgtgtacaca atattattta aaatttaacc atctaagaaa
     3121 ggttgttggg tctttattcg tttagcaata agtcgtagta taaacgcaat tagggctgta
     3181 aggaaggcaa aaattgccat tagatcaaat cctaccaggt agttctgtgt ttgatcataa
     3241 gaaaatccaa ccagtaaagg ccccaaaagt ataccgatat tcagacaggt tgatagtatg
     3301 ccatagccca gacccacctt agaaagagaa atatatttgg gcaccagggc aaataccggt
     3361 gacggaattg atgcagcaaa aaatcctatt agacctccaa ttattaatgg gttaaacaat
     3421 gttctgggaa caacatataa taaaagtgct atggcaatac ttccaacaat aataaaatat
     3481 tcctctctgc ccactctgtc tattaaagtt cccaccaagg gactgaataa aagtgatcct
     3541 accattaaga gactggttaa aaaaccggca tagctaacat catagcctac agactgatag
     3601 taatcagcgc caaaggtgag ataggagatg gtcgatgcgt tatacatcat ccatattgca
     3661 gcaaccaacc atacctgagg tccagttttt ctaagtgtaa aaactttttc ctcaaattct
     3721 ggttgttcct gccttcgaat ttcatcaggc agtttcggat atctaaaata aaataaaacc
     3781 agcacgccta aacagaaaaa tgaagtcaaa aataggggga cctgccaggt ataaataccg
     3841 gccaggacac taaatacgtt taaagtaaag atacttccta aaggcatagc agtattaaaa
     3901 atccccattg cctttcctaa atctctactg gcaaaccagc gagacagggc ttgtggtgcc
     3961 acaatagaaa tggttaaacc gccgatacct gaaatgatac gtcctgttac cagcaaagaa
     4021 aagtcttttc ccagtccaac caataaggaa ccgattagtg taattagtaa agcagttacc
     4081 cctacccgtt taggtccata aacatcggcc aggattccac ctggtatgga aactaaaatt
     4141 cctggcagac cgaacagact catcagggcc cctgcctggg tatgggaaat tcccaatgat
     4201 tgcactaaaa atcctaaaag cggagggata ctctggaagg ttaaagcaaa gaccagcata
     4261 aagaaataaa tgtaagccag tgctatccag cgttcctgat atttcatcct ctccctcccg
     4321 ctatacgcct taatatgagc tgaaattgtc tttgccattc tatgttgata tgatacagag
     4381 aagcaattct tttaataact tcaaagttct gtctttaata tcatagtatt ctgtttaata
     4441 tctcagagtt aaataacctt acctcgatta ttattttttg ctttttggta tacatagaac
     4501 gctgctgcaa tatcctcgat tgctaatcct aatgccttaa aaatagtaat atctatatta
     4561 ttgcttcttc cggctatact atcagttaat agatcgccga tttcgcccaa tatatggtta
     4621 ttatttataa ttccttcctg ttttggtatc aaaaaatccc ctgcctcatt tatggtcgat
     4681 tccacaaaat caacatataa ttttgatttt aagataattt ctgaatccaa ttctctggca
     4741 tccggagaac acgctcccac cgcattgata tgggttcctg gtttaaccca tttaccttct
     4801 aataccggtg tttttgatga tgtaaccgta catatcacat cagaattaca tacagcttct
     4861 tctgcgtttt cagcttcaaa aatgttcaca tcatattgtt ttttcatctc ttgaacaaac
     4921 aaacgggaat aatctttatt tacatcccat accctcacct ctgtaaaatt tcttaccagt
     4981 agcattgctt ctaaatgtga cctggcctgc tctcccgcac ctaaaatagc aagtgtagaa
     5041 acatcttttc ttgaaagcag ttctgtggca acagcactaa cggcggctgt tcgtatcttt
     5101 gttattgctg aggcatctag aaaagctttg atatagccat attgtgcatc aaatagcaat
     5161 accataccct gatgggaggg gtatccgctg cccatatttg ttttaaagac ggtgatgatt
     5221 tttgcaccaa aataaccgct tttaccggaa taacagggca taaaacctaa aatattacca
     5281 tccggtaaat gtgttgcagt tctaagatat tgagctactt ccccgtttga taatcttatt
     5341 aaggcatctt tcataacatc aatacattca ctcatgctca ttagctctaa tacttcatca
     5401 taacctataa atagcaccat actttatctc ccaatataat tattatcgct ttgaataatt
     5461 aaacatttag taaaatgaac tgccagtaca actgtctcct taaattattt cttcaataat
     5521 ttttcttgtt acccaataaa taatctttct gtaatttcaa taatttgtaa taatgtttta
     5581 tgcactatct gtttctagta gatattctat aaccttaata attgtcaatt ctaacccaat
     5641 aaatcttgtc tgtaacaaat agtcaaactt caggattgtt ggggcaataa tttgctttga
     5701 acaattatat tatagtttat ctgactatgg ttggcaaaaa tggtattcaa ttgtttggag
     5761 aggtctctga cactttattt atgcacatat tcaggttcag ggtaatactg taatggtacc
     5821 aatagtcagg ttaatgccag caccagaacc tgatacttct acagtgaatg atttggtaac
     5881 agtagtatat ttattatctc gaatactaac tgttattagt tgatccattg ttataactct
     5941 agtatctttt tctctgtaac cagaaacaat tgctatatca ttttcggcat ccggtgtaag
     6001 tattaaacag gtcatattgc aggggaaatt tgttataacc ttcgcctgta attcattgcc
     6061 aaggttaacc caggaagtag tggaaccacc tttaattatc attgcctcag tgcttccagt
     6121 caccacatct cgataattag aggttatagt tatctgatga ttagctgaat ctgcttctat
     6181 ggtcaacgcc cgggcgacta tagacaaatg aacagtatta attgttttac cggaagcagt
     6241 atgggttata acaattattt cgccatggtc cgttatggac agttctgttc catcctctgg
     6301 agaggcagtt atgccttttt ccccgaaatc tgcaaacact acatcctcag ttgaattgtt
     6361 attatagagc aaagtaacta ccagaccttc cagttttagg ttctgacctt ccacgtagtt
     6421 caatctgtcg ggctggcttt tgacagttat acctgttact gctatttcct caaatatggc
     6481 ttccaccgtg atatctttgt ttacattggt atccatacgg gggttatctt gagaaccatc
     6541 actccatttt acgaaataat aaccttcatc aggtaatgct tcaaccgctt taccatcatt
     6601 tccatattct actgcttgta atatttcccc gatgacactc ccatttgcac tgtgattata
     6661 ggttagtgta caaattttta ccttaaatgt tatatccctg gtagaaactg ctgaattggt
     6721 atatcctctt ctggtaccca tggcttttac tataatgatt tcaccctcag gtttagctgt
     6781 gcttactata aatggtgccg tatattttgt tgaatttgaa gacggggtta acccattcaa
     6841 ggtgtagtag atgactgcat ctggtgttgc tgttgtta
//
LOCUS       DAOQWT010000018         6355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_12606_length_6355_cov_8.392857, whole genome shotgun sequence.
ACCESSION   DAOQWT010000018 DAOQWT010000000
VERSION     DAOQWT010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12606_length_6355_cov_8.392857"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1020
                     /locus_tag="PKJ95_00870"
     CDS             190..1020
                     /locus_tag="PKJ95_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR64823.1"
                     /translation="MNGNGLLTIKEASELVGINQTTIYRKINKGQLHATAVQLQGIEV
                     KKVKKDDIIRLFGNYKNSEQLHATATQVQEATKQLHATATQMPSSEERKSEIREVIEQ
                     FFSVKQAELVKPIEQQSLYRLGRVEQENNFLKAKVETLLEEIEKYKALPWQIEERDNE
                     IKELEARHQQEKEKILKECETKLLEAKDEKIKTEEAFKEKLKILPAPVESINQILLEN
                     AHNLKILQKQTTELIEKLKEEEEEKEKIKTEAEEAQKAIVEAWKKELELAKKPWWKLW
                     "
     gene            1337..1555
                     /locus_tag="PKJ95_00875"
     CDS             1337..1555
                     /locus_tag="PKJ95_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64824.1"
                     /translation="MNLLEKIYNNLNHEKIWGSDGLPPLNNFKSNSGGFAACCPNPAH
                     PKKKTIFPHERRHPCRQMPVLRISSHMV"
     gene            1667..4159
                     /locus_tag="PKJ95_00880"
     CDS             1667..4159
                     /locus_tag="PKJ95_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR64825.1"
                     /translation="MVMNFDLIINKFSNVKRLSNGSYQCSCPLPAHKHGDKTPSLTIT
                     EKEDKILIFCHVCGESATPEILKTAGLEEKDLFKKNWNKEFSSEYSSSGGDKKTYPLD
                     WIVKNSTVQYFYNNDNNEPEILKIRYLDKTTGEEVKGCFYHYAGCDRWIKNLNGKRPQ
                     LYKLPELLQAIKRGEEFIFDVEGEKDVETLSKVGLTATTSGSSKSWQDKFKEYYRGAK
                     KVIILPDNDQPGKEYAGQKAKSLHGIAEEVKIVELPGLKEREDVTDWINGGHTKEELL
                     ELVKNTPVYSPAAPPVEQKEKKKTLLDYDLKPGDLNIPEEWLIKDILAPYTINLYYSA
                     SGAGKSFYWLLKSIYLLKEKKVDTIIYLDNDNSKRALKSRYIAEIKAHYKNFIYIPSF
                     KVDNELMTTLEEELKTKKHGHSLIIVDSIRNFMVGKNPNEDKDSISFMDKMKYLRSLD
                     NTVVMLHHVNKMKQIKNNTSFVDYSDVVYQMIANNDKQKKRIFLEFSCEAEKGKDRIG
                     SKETFTALLDYDILQLIVQEAGVNPEDFDFVSYVRDLLTEYGYLNQRTICEYAKHNMS
                     MRTEDAFILLKKYTGILWNFERGVKNSCIYSLKEEVDNNNILYICLNDIKNSGKLENS
                     TKSTDSAETPSGKLENSDTANVTDDLTCGKLENTVKSIVSDNTGVNIDFNEVNSIVKQ
                     NFGLNTQFSSLPEVNRKETGRTIEFSSFPAFLREDNLIAGNQEITPLLLEQVVQLVLG
                     SIELNFCRDNNNSFFNGQTFRELSSSSNCEVVSCIDCQYFSGKAGAVCSVTGKKHLAH
                     QEHLCQKFSPAIRDMQNYYSEEEEEEIIDAPF"
     gene            4288..4509
                     /locus_tag="PKJ95_00885"
     CDS             4288..4509
                     /locus_tag="PKJ95_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64826.1"
                     /translation="MLESEFGICLFLIFIKKIDVEKPCYDWHYHLNAKKSRFVLCTPL
                     QPACTDPASPRVTVSTGTAPQIWHVDYIR"
     gene            complement(4533..5000)
                     /locus_tag="PKJ95_00890"
     CDS             complement(4533..5000)
                     /locus_tag="PKJ95_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64827.1"
                     /translation="MNNKKQSKRHGEEPGKQKNELPNWQPISMIAMIASIIDGEVENF
                     EDLYKNLCEARKKPHVMDDMIIDRAINNHKKYLKEAWVYDEQIARWKKEKLTDEQGRE
                     IERLTGQMLKYRKMCEEIITVSEEVKKGTINRILEMSDEEVAIAVLTGKLKMP"
     gene            complement(5367..5621)
                     /locus_tag="PKJ95_00895"
     CDS             complement(5367..5621)
                     /locus_tag="PKJ95_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64828.1"
                     /translation="MGGEGAAGYNGCAAIPGYLLQQKLKRSRPWLRLQHIFYYALQAA
                     QHRFCFLPAAAPIFAMVRICGAMPGKPASPGLYRTGASRI"
BASE COUNT         2191 a         1334 c         1122 g         1708 t
ORIGIN      
        1 tggcacgatg ttaaattttg tcattttctg tttttatacg ttgccagatt cgcattaaac
       61 cctttagata aaagcaggaa actttattga tctgtggaat atgaaatata ttaataaaat
      121 aaagcaggtg aagatgtctt gttataaggc aattcacctg ctttattatt tccgatggag
      181 gataaatata tgaatggaaa tggattatta actataaaag aagcttcaga acttgtaggt
      241 attaaccaga ctactattta cagaaaaata aataaagggc aactgcatgc aactgcagtg
      301 caactgcaag gaattgaagt aaagaaagtt aaaaaagatg atattataag gcttttcggc
      361 aattataaaa atagtgagca actgcatgca actgcaacgc aagtgcaaga agctacaaag
      421 caactgcatg caactgcaac gcaaatgcct tcaagtgagg aacgaaaatc agaaataaga
      481 gaagtaatcg aacaattctt ttctgtaaag caagccgaat tagtaaaacc cattgagcaa
      541 caaagtttat atagacttgg aagagttgaa caagaaaaca attttttaaa agctaaagta
      601 gaaacacttt tagaagagat agaaaagtat aaagctttac catggcagat agaggaaaga
      661 gacaatgaaa taaaagagct tgaagcacga caccaacaag aaaaagaaaa aattcttaag
      721 gaatgtgaaa ctaaactgct tgaagcaaaa gatgaaaaaa taaaaacaga agaagccttc
      781 aaggaaaaac ttaaaatctt accggcacct gtagaatcaa taaatcagat cctcttggaa
      841 aatgctcata atctgaaaat tctacagaag caaacaacag aacttataga aaaacttaaa
      901 gaagaagaag aagaaaaaga aaaaataaag acagaagcag aagaagctca aaaggctatt
      961 gtagaagcct ggaaaaaaga acttgaactt gctaaaaagc cctggtggaa gctatggtaa
     1021 aagttatttc aaaaaataaa acaacataaa gaggattgta acaactaaaa atagaatcaa
     1081 tctgtgggtt ttggtttttt tatggggtaa ggaatacaga gtaaatttta aacgattggc
     1141 taaatgaaag aaactgagtc tgcaaactca gtttcaagaa aaaaattcat tccgtagaca
     1201 atcgcttaat ttaactacca tcacccacct cctaacttca aaatgtaggg ggtagttaag
     1261 tattctattt atataaatta tacacattat aacaaaatcc ttcaaacaca tataagaaaa
     1321 aggagaaatg ttagctatga atttattaga aaaaatatac aataatctaa atcatgagaa
     1381 aatatgggga tctgatggat tacccccctt aaacaatttt aaatccaact ccggcggttt
     1441 tgctgcctgc tgcccaaacc cggcacatcc gaaaaaaaaa accatctttc ctcatgaaag
     1501 aaggcatccc tgccggcaaa tgccagtcct gcggatatca tctcacatgg tttgatgcag
     1561 cagcaatgaa cttaacgccc ttcggacgaa cttggcttaa agctctattt tacgccgtaa
     1621 agaatgaaaa ttcctataca tttaaaataa agaaaggaga aagtaaatgg ttatgaattt
     1681 tgatttaata atcaataaat tcagtaacgt aaaaagactc agtaacggca gttatcaatg
     1741 ttcttgcccg ctgccggcac acaaacacgg ggataaaacc ccgtctctga ctataacaga
     1801 aaaggaggac aaaattttaa tcttttgtca tgtctgcggt gaatctgcaa caccggagat
     1861 actaaaaact gcaggacttg aagaaaaaga tttgttcaaa aaaaattgga acaaagaatt
     1921 ttcttcagaa tactcttcat ccgggggaga taaaaaaacc tatcctctgg attggatcgt
     1981 taaaaactct acggttcaat acttctacaa taacgacaat aatgagcctg aaatcctgaa
     2041 aatccgatat ctggataaaa ccactggaga agaagtgaaa ggctgcttct accactatgc
     2101 cggttgtgac agatggataa aaaacctcaa cggcaaaaga ccgcagcttt ataagcttcc
     2161 agaactactg caagcaatca aaagaggaga agaattcata tttgatgtag aaggagagaa
     2221 agacgttgaa actctttcaa aagtcggtct tacagcaact acttccggca gttcaaagtc
     2281 atggcaggat aagttcaaag aatattacag aggagcaaaa aaagtaatca tactgcccga
     2341 taacgaccag cccggcaaag aatatgccgg acaaaaggca aaatctcttc acggaatagc
     2401 agaagaggta aagatagtgg aacttcccgg attaaaagaa agggaagatg taacagactg
     2461 gattaatggt ggacacacaa aagaggagct cctggagctg gtaaaaaata ctccagtata
     2521 ttctccggca gcaccaccag tagaacagaa ggaaaaaaag aaaacccttc tcgactacga
     2581 cttaaagcct ggagatctga atatccctga agagtggtta attaaagata ttcttgcacc
     2641 atatacaatt aacctctatt attccgcatc aggagcagga aaaagctttt actggctatt
     2701 aaaatcaatc taccttttaa aagaaaagaa agtggacacg atcatctatc tggataacga
     2761 caacagcaaa agagctttaa aaagcagata cattgcagaa ataaaagctc attacaaaaa
     2821 ctttatttat attccttcat ttaaagtaga taacgaatta atgactactt tagaagaaga
     2881 attaaagact aaaaaacatg gccattctct tattatcgta gactccataa ggaactttat
     2941 ggttggtaaa aacccgaatg aagataaaga ctctatttca tttatggaca aaatgaaata
     3001 tttacgaagc ctggacaaca cagttgtaat gcttcaccat gtaaataaaa tgaagcagat
     3061 aaaaaacaac acctcctttg tagattactc ggatgtagtc tatcaaatga tagcaaataa
     3121 cgataaacaa aagaagagaa ttttcttaga gttctcctgc gaagcagaga aagggaaaga
     3181 caggataggg agtaaagaaa catttactgc tttacttgat tatgatatcc ttcagttaat
     3241 agtccaggag gccggagtaa atcctgaaga ttttgatttt gtttcttatg taagagatct
     3301 gctgacagaa tatggatatc tgaaccagag aacaatctgt gaatatgcaa aacataacat
     3361 gtcaatgagg acagaagatg catttatact ccttaaaaaa tataccggca tcctctggaa
     3421 ctttgaacgc ggagtaaaaa actcttgtat atattcctta aaagaagaag tagataataa
     3481 taatatatta tatatatgtc ttaatgatat aaaaaactct ggaaaactgg aaaactcaac
     3541 aaaatcaacg gattctgcag aaacaccctc tggaaaactg gaaaactcag atactgcaaa
     3601 tgttactgat gatttaacct gtggaaaact ggaaaacaca gtaaaatcaa tagtttctga
     3661 taataccggc gtaaatattg actttaacga agttaattct attgtaaagc agaattttgg
     3721 cttaaacact cagttctcca gtttaccaga agttaataga aaggaaaccg gcagaactat
     3781 tgagttttcc agttttccgg cctttttgag agaagataac ctgatagctg gaaatcagga
     3841 aattacccct ttacttttag aacaggttgt tcaactggtt ttaggatcta tagaactaaa
     3901 cttttgtaga gacaataata attcattctt caacggtcag actttcagag aattatcttc
     3961 atcttctaac tgtgaagtag tgtcctgtat cgactgtcaa tatttttccg gtaaagccgg
     4021 ggctgtctgc agtgtaacag gaaagaagca tttagcccat caggaacatc tttgtcagaa
     4081 attttctcct gctattagag atatgcagaa ttattactca gaagaagaag aggaagaaat
     4141 aatagatgca ccattttaat aagagttgaa aataaactca gagttattat taatttagaa
     4201 ctataaaaca aaaatatttt aaaggtttta aagtctattt gctatggctt ttcgcttgca
     4261 agaatagcta tgctgctata ggattctttg ctggaatctg aattcggaat atgcttattt
     4321 ttgattttca ttaaaaaaat agatgtcgaa aaaccttgct atgactggca ttatcacctt
     4381 aacgccaaaa aaagtcgttt tgtactctgc accccgttac agcctgcctg taccgatccg
     4441 gcaagtccca gggtaacagt ttccaccggc acagctccgc agatctggca cgttgattat
     4501 attagataat ttgtttattt cattcacccg atttaaggca tcttgagttt cccggtcagg
     4561 acggcaattg ccacctcttc atcgctcatc tcaagaatcc tgttaattgt acctttcttg
     4621 acttcctctg acactgtgat gatttcttca cacatcttcc gatatttcaa catctgtccg
     4681 gttagtcgct ctatctcccg gccctgttca tcggtaagtt tctctttctt ccaccgggca
     4741 atctgttcgt cataaaccca tgcttctttc aggtattttt tgtgattgtt tatggcacga
     4801 tctattatca tatcatccat tacatggggc ttctttctgg cctcacacag atttttgtaa
     4861 agatcctcaa aattctccac ttccccatca ataattgatg caatcatagc tatcatgctt
     4921 attggctgcc agttcggtaa ttcattcttt tgctttcccg gctcttcgcc atgcctttta
     4981 ctttgctttt tattgttcat cctgaacctc ctctgcacat atcaagaatg gttaaatact
     5041 atcacaggtt attgggtttt acaatataag atacaatctc tcaaaggaat taaatttaaa
     5101 tccggccctc tccccctccc cacccatcag ccccggcaac atcttattat accggccatt
     5161 gcatacaaac cctacatctc cagctgctta aacccggcga tctcgctcca gccaaactca
     5221 gggagaagac tcgctgttac tcgctttcca gatattccct ctaaagccga cgcaaatctt
     5281 ttcgcagtag ctcaaagact tgctgcacaa gacgttctac ttacaaaagg caaaccgacg
     5341 cgagacttct ccctgatatg aggccgttat atcctgcttg caccggtccg atataaccca
     5401 ggagaagcag gttttcccgg catagctccg cagatccgaa ccattgcaaa aataggagct
     5461 gctgcaggca aaaagcagaa tctatgctgt gcagcttgca gagcataata aaagatatgc
     5521 tgcaggcgga gccagggccg gctacgcttt aacttttgct gcaggagata tccgggaata
     5581 gcagcacagc cgttatagcc ggcagccccc tccccaccca tcagaatcct aatgtcatat
     5641 ttttaccgaa ctttgccgat aaccgttatt accagcaacc cgcccggatt tgattttctg
     5701 cccgccccta aaccgccctt gaaaatcaaa tcttcttccc acccatcata acccttctac
     5761 cggattctta ctgaagtttg cagcaagccg ttattcccgg caacccgccc ggatttgatt
     5821 ttctacccgc ccctaaaccg cccttgaaaa tcaaatcttc ttcccaccca tcataaccct
     5881 tctaccggat tcttactgaa gcttgcagca agccgttatt cccgacaacc cgcccggatt
     5941 tgattttcta cccgccccta aaccgccctt gaaaatcaaa tcctcttcct accctctaaa
     6001 ccgttctgtc atgcttttac tgaagcttgc agcaagccgt tattcccgac aacccgcccg
     6061 gatttgattt tctacccgcc cctaaaccgc ccttgaaaat caaatcctct tcccaccctc
     6121 taaaccgttc tatcatactt ttaatgaagt ttgcagcaat ccgttattcc cggcaacccg
     6181 cccgtatttg attttctgcc cacccctaaa ccgccccaga aaatcaaatc tccttcccac
     6241 cctttcaacc gttctatcag gcttttaatg aactttgctg ataaccgtta gtgcctgcaa
     6301 cccgccccgt ggcgagcttt cgacgcgtct caaacttcgc cacctgtccc cctga
//
LOCUS       DAOQWT010000019         6089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_13252_length_6089_cov_2.637057, whole genome shotgun sequence.
ACCESSION   DAOQWT010000019 DAOQWT010000000
VERSION     DAOQWT010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13252_length_6089_cov_2.637057"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PKJ95_00900"
     CDS             complement(<1..705)
                     /locus_tag="PKJ95_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNR64829.1"
                     /translation="MSPEKENIQLVQDVATPCNAPAAIANAFCTIGKTKASLSLAKMI
                     ILGILAGAYISFGAQLATMATYDTAKYVGDGIARVLFGSVFSVGLMLVVIAGAELFTG
                     NNLIVVGTLNKNITVEKLLNSWFWVYIANFVGSLLVVWLMYLAKLWTTGNFAVGARAL
                     AIANGKVNITWGAAIARGILCNWLVCLAVWMAVAAKDVPGKILGIYFPIMAFVASGFE
                     HSIANMYFIPMGILLKN"
     gene            complement(745..3480)
                     /gene="fdhF"
                     /locus_tag="PKJ95_00905"
     CDS             complement(745..3480)
                     /gene="fdhF"
                     /locus_tag="PKJ95_00905"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNR64830.1"
                     /translation="MPMVQVTINDQKVMIEPGSTILDAANKAGIKIPTLCAMSEINHY
                     PGSCRMCVVEVKGHPCLVASCVYPVNEGMVIYTHSDRVVNARRAVLELLLTTHPLDCM
                     TCEKNGECDLQDLAYEFGIKESGFGRKERNLPIDNSNPFILRDLNKCILCRRCVEICN
                     EIQQSRAIGFGFRGTKTEVIAGLGHKLGHETKMTIGSKDNPDYSNCVSCGQCVAVCPV
                     GALTDKAAEGKGRVWEFQKVHTTCNYCGCGCGFDLNIKDGKVVKVTSNSDSIVNGINL
                     CVKGRFGNDYIHKEDRLKTPLIRKNGKLEPASWDEALTLISNRFQEIKKESGSDSLAA
                     LSSAKCTNEENFLLMKFARAVFGTNNVDHCARLCHSATVAGLAQSFGSGAMTNSIKEI
                     ANAPVIYLTGSNTTENHPIIALEIKKAVTKNGAKLIVADPREIELVKYATLWLRHRPG
                     TDVALFNGLMNVIIMEGLEDKEFIKERTEDYEKLREVVLKYTPEIVAKITGVPAEDIR
                     KAARIYASAESASLIYSMGITQHSTGTDNVMSTANLAMLTGNVGKENAGVNPLRGQSN
                     VQGACDMGALPNVYSGYQAVTDPQAREKFSKAWGVELPDKIGLTVVEILNAAYDGKIR
                     GIFVMAENPAMSDPDLAHAREALKRVDFLVVSDIFLTETAEVADVVLPGVTFAEKDGT
                     ITNTERRVQRSHKAIEPLGEAKPEWQIICELAQKMGYQMSYNSPAEIMEEIASLTPIY
                     GGILYPRLGKEGLQWPCPNMEHPGTRFLHKDKFSRGKGKFIPIEFKEAVELPDEEYPL
                     ILTTGRVLYHFHTGTVTRRSKGLNEIYKEALVEISPQDAQELNITDGEIIEVASRRGK
                     VRAKAKVTEKSDEGVVFMSFHFHEAAANLLTIAALDPISKIPEYKVCAVKVKKIS"
     gene            complement(3552..>6089)
                     /locus_tag="PKJ95_00910"
     CDS             complement(3552..>6089)
                     /locus_tag="PKJ95_00910"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNR64831.1"
                     /translation="RGRGGAGFPTGLKWEFAAKASGQPKYILCNADEGDPGAFMDRSM
                     LEGDSYSVLEAMMIGGYAIGANQGYIYVRAEYPLAIERLRIAINEMKKHNLLGKNILG
                     TGFDFDVEIRLGAGAFVCGEETALMHSIEGKRGEPRPRPPFPANKGLFQSPTVLNNVE
                     TLANIPYIILYGAENFASVGTEKSKGTKVFALAGNINNTGLIEVPIGITLGEIVYNIG
                     GGIPGNKKFKAVQIGGPSGGCIPAQHLNAVVDYESLKELGAIMGSGGLIVMDEDTCMV
                     DIARYFMEFIQDESCGKCTPCREGTRVMLEILTRICEGKGKMEDLDTLEELSYRIQDT
                     ALCGLGQTAPNPILSTLRYFKHEYIEHIRDKKCRAGVCAELVYAPCTNSCLASVNVSA
                     YLAYTKEGQYEKAMEAHLRTNPFPAVCGRVCPAFCETRCRRRDIDDAVNIRAVKRFMA
                     DQVDNYLACFPEKNRKNGKKVAIIGGGPSGLSNAYFLTMLGYETTVFEAQPKAGGMLT
                     YAIPSYRLPKNIVDKEIQALIDYGVEIKTNTIVGEDITLDQLRKEGYQAFYISAGAWD
                     SIMPEMDNLENPRIFSGLEFLSRFNRKEKLDVGKEVMVIGGGNSAIDAARTAKRLGAN
                     VTIVYRRTREEMPADEAEINEAEREGIAISLLQNIKSVKSENGYLEVELVNMKLGDYD
                     ASGRRRPVENKGSNFNKRVDSLILSIGQKPALSELFNNGEVNLNRDQTISTEKGITNI
                     RDIFAGGDVALGPATVVEAIGDAQNAAEAIDFYLTGKKRVYPWRVKYPINVDFDPEAD
                     PVDYARSHDNLIPVVERGIHDEVEKTWSKEIACRESSRCLRCEYRED"
BASE COUNT         1673 a         1462 c         1032 g         1922 t
ORIGIN      
        1 gttcttgagt agaattccca taggtataaa atacatattg gcaatgctat gctcaaaacc
       61 actggctaca aaagccataa tgggaaaata tatgcccagg atttttcccg gaacatcttt
      121 agctgctact gccatccata ctgccagaca caccagccag ttacagagaa ttcctctggc
      181 tattgctgca ccccaggtta tattgacttt accatttgct atcgctaaag ctcttgcccc
      241 tacagcaaaa tttccggtgg tccaaagttt tgcaagatac attaaccata ctaccagcaa
      301 agaaccaaca aaattggcta tatataccca aaaccagctg tttaataatt tttctacggt
      361 tatatttttg tttaaagtac ccactacaat taaattatta ccggtaaaca attccgctcc
      421 agcaataact accagcatca agcccactga aaagacactg ccaaaaagta ctctggcaat
      481 accatcgcca acatatttcg ctgtatcata agtggccata gtggcaagct gtgctccaaa
      541 gctgatataa gcaccggcta gaatacctaa aataatcatt ttagccagtg atagacttgc
      601 cttggtcttg ccaatagtgc aaaaagcatt ggcaattgcc gcaggtgcat tgcatggtgt
      661 tgctacatcc tgtaccagct gaatattttc tttctctgga ctcaatttga tttcctcctc
      721 ttttgacaat atattattat atttttagga tattttttta actttaactg cacatacttt
      781 atattctggt atcttggaaa tgggatcaag agctgcaata gtcaacaaat ttgccgcagc
      841 ttcatggaaa tggaagctca taaataccac tccctcatct gatttttctg taaccttagc
      901 ctttgctcta acctttcctc tgcgagaggc aacttcaatt atttccccat cagtaatatt
      961 tagctcctga gcatcctgag gactgatttc cactaatgct tctttgtaaa tctcgttcaa
     1021 ccctttggac cttcgagtaa ctgttccggt atggaaatgg tacaatactc ttcctgtagt
     1081 taggattaac gggtattctt catcgggcag ttcaaccgcc tctttaaatt caattgggat
     1141 aaactttcct tttcctctgc tgaatttatc cttatgtaaa aacctggtcc ccggatgttc
     1201 catattggga cagggccatt gcaggccctc ttttcccaat ctgggataga gaatccctcc
     1261 gtaaatagga gttaaactgg caatttcttc cataatttca gcagggctat tataactcat
     1321 ttgatagccc attttttgag ccagctcaca aattatttgc cattcaggct tggcttctcc
     1381 taaaggttct atagctttat gggatctctg cactcttctt tcggtattgg tgattgttcc
     1441 atccttctca gcaaaagtaa ctccgggtaa taccacatca gctacctcag ctgtttcggt
     1501 caaaaatatg tcagatacca ctaaaaaatc aaccctcttc agagcttcac gggcatgtgc
     1561 caaatccggg tctgacatag ccggattctc agccattaca aaaattcctc tgattttacc
     1621 atcataggca gcatttaata tttctaccac cgttaatcct attttatctg gtagttccac
     1681 tccccaggct ttagaaaatt tctctctggc ctgcgggtca gtaacagcct gataaccgga
     1741 atagacgttg ggcagtgctc ccatatcgca ggcaccctga acattgcttt gcccgcgcaa
     1801 tggatttaca ccggcatttt cttttccaac attaccggtt aacatggcca gattagccgt
     1861 agacattaca ttgtcagtac cggtgctgtg ctgagtaatt cccattgaat agattaaaga
     1921 agcactctca gcactggcat aaatccgggc agcttttcta atgtcttcag ccggcacacc
     1981 ggtaattttt gctactattt ccggggtata ttttaagact acttctctca acttttcata
     2041 atcttctgtt ctttctttaa taaattcttt gtcttccagt ccttccatga taatgacatt
     2101 cattaaacca ttaaagagag ccacatcagt tccaggccta tgtcttaacc agagtgtggc
     2161 atatttaact aattctatct ctctggggtc tgccacaatc aattttgctc catttttggt
     2221 aactgctttt ttaatttcca aagcaattat aggatggttc tcagtagtat tggaaccggt
     2281 cagatagatt accggtgcgt tggcaatctc tttaatagag ttggtcatag ctccacttcc
     2341 aaaagattgt gccagaccgg caaccgtagc agagtgacag aggcgcgcac agtggtccac
     2401 attattggtt ccaaaaacag cccgggcaaa tttcataagg agaaaattct cttcattagt
     2461 acatttagct gaagaaagtg cggccaggct atcactgccg ctctcttttt taatctcctg
     2521 gaacctatta ctgattaagg taagcgcttc atcccaggaa gcgggttcca gcttaccatt
     2581 ttttctaatt aacggagttt ttaagcgatc ctctttatga atgtagtcat ttccaaaacg
     2641 acctttcaca caaagattaa ttccatttac tatactatca gaattggaag ttactttaac
     2701 tacctttccg tctttaatat ttaaatcaaa tccgcatcca catccacaat aattacaggt
     2761 agtatggact ttttgaaatt cccacaccct tccttttcct tcagctgctt tgtcagttaa
     2821 ggctccaaca gggcataccg caacacactg gccacaggaa acacaattgg aataatcagg
     2881 attatctttt gaaccaatgg tcattttggt ctcatgccct aatttgtggc ccaaaccggc
     2941 tattacctct gtttttgttc cacgaaagcc gaatccaatg gctcttgatt gctgaatttc
     3001 attacatatt tcaacacatc tccgacaaag aatgcattta tttaaatctc ttaaaataaa
     3061 agggttgctg ttatcaatgg gaagatttct ctccttcctg ccaaagccgg actctttaat
     3121 cccgaattca taggccaaat cctgtaaatc acattctcca tttttttcac aggtcataca
     3181 atccagcggg tgtgtggtta ataacaattc caatacagcc cggcgagcat taaccactct
     3241 atcgctatga gtatatatga ccatcccttc atttaccggg taaacacatg aggctaccag
     3301 acagggatga cctttaacct ctaccacaca catacgacag gatcctggat aatgatttat
     3361 ctctgacata gcacataatg tgggtatttt tataccggct ttattggcag catccagaat
     3421 tgtagagcct ggttctatca taactttttg gtcatttatt gtaacttgaa ccataggtat
     3481 ttctgttttt ttatctttta tttctgtagc tttcatttta tcttattcca ttccttcctt
     3541 tctcttcaaa attagtcttc tctgtattca caacgcaggc atctgctgct ctcccggcaa
     3601 gctatctctt tactccaggt tttctccact tcatcatgga tacctctttc gacaacaggg
     3661 atgagattat catgtgaacg ggcataatcc accgggtcag cttcggggtc aaagtccacg
     3721 ttgataggat atttcaccct ccagggataa actctcttct tgcctgttaa ataaaaatca
     3781 attgcttcag cagcattttg agcatcacct atggcttcaa ctactgtagc aggtcctaat
     3841 gcgacatcgc caccggcaaa tatatctctt atattagtta tacctttttc agtggaaata
     3901 gtctgatcac gattaagatt tacttctcca ttattgaaca attctgaaag ggcaggcttc
     3961 tgcccgatgg ataagatcag ggaatcaact ctcttattaa aattggaacc cttattttca
     4021 acaggtctgc gtcgccctga agcatcataa tcccctaatt tcatatttac cagttcaact
     4081 tccagataac cgttttcaga ctttactgat tttatattct gcagtagaga aatggcaata
     4141 ccttctcttt ctgcttcatt tatttcagct tcatcagcag gcatttcctc tctggtcctt
     4201 cgataaacaa tagtaacgtt tgctcctaac cttttagcag ttctggcagc atcaatagct
     4261 gaattgcccc caccaattac cattacttct ttaccaacat cgagtttctc ttttctgtta
     4321 aatctggata agaattctaa accgctgaaa attctggggt tttccaaatt gtccatctca
     4381 ggcataatag aatcccaggc accggctgaa atataaaatg cctgataccc ttcttttcta
     4441 agctgatcta aagtgatatc ttctcccaca attgtattgg tttttatctc aactccataa
     4501 tctatcagag cctgaatttc tttatctaca atattttttg gtaaacggta agaaggtatg
     4561 gcataggtca acattccgcc agcttttggc tgggcttcaa atacggttgt ttcatatcct
     4621 aacatagtta gaaaataagc attagagagt ccggaaggac caccaccgat gatggcaact
     4681 tttttaccat ttttcctatt tttttcaggg aaacaggcca gataattatc aacctgatct
     4741 gccataaatc tctttacagc acgaatattg acagcatcat caatatctct tcgacgacat
     4801 ctggtttcac aaaatgcagg acataccctt ccgcataccg ccgggaaggg atttgttctt
     4861 aaatgcgcct ccatagcttt ctcatattgt ccttctttag tgtaagccag ataggcagat
     4921 acattaaccg aggcaagaca ggagttagta cagggcgcgt ataccaattc tgcgcaaact
     4981 ccagctctgc atttcttatc tctgatatgc tcaatatatt catgtttgaa atatcttaag
     5041 gtggacaaga tcgggttggg agcagtctgc cccagaccac aaagagctgt atcctgaatc
     5101 ctataagata attcctctaa ggtatccaaa tcttccatct tacctttgcc ttcacaaatc
     5161 ctggtcaata tctccagcat caccctggtc ccttcacggc aaggagtgca ttttccacag
     5221 gattcatcct gaatgaactc cataaaatag cgggctatgt caaccataca ggtgtcctca
     5281 tccataacta tcaacccgcc tgagcccata atagctccaa gttcttttaa tgattcataa
     5341 tcaactactg cattgagatg ctgtgccgga atacagccac ctgatggtcc tccaatttga
     5401 acagctttga attttttatt tcccggaatt cccccgccaa tattgtaaac aatttcacct
     5461 aaggtaatgc cgatgggaac ttcgattaaa ccggtattgt taatatttcc ggcaagggca
     5521 aataccttgg tccctttgct cttttcagtt cccacagaag caaagttttc agctccataa
     5581 agaattatat aaggtatatt ggctaaggtt tctacattgt ttaaaacagt aggagattga
     5641 aatagccctt tgttggcagg gaaaggtggt cttggtctgg gctctcccct cttaccttca
     5701 atggagtgca ttaaggcagt ctcttcacca cagacaaatg ctccagctcc taatctaatt
     5761 tcaacatcaa aatcaaagcc agttcccagg atatttttac cgagtaaatt atgtttcttc
     5821 atctcattaa tagctattct taatctttca atagctaagg ggtattccgc tcgaacgtaa
     5881 atatatccct ggttagcacc gatagcataa ccgccaatca tcatagcctc caatacgctg
     5941 taggaatccc cctccagcat actgcggtcc ataaatgcac ccgggtctcc ttcatctgca
     6001 ttgcataaaa tatattttgg ctgaccggat gctttggcag caaattccca cttcaatcct
     6061 gttggaaagc cagctccacc tctgcccct
//
LOCUS       DAOQWT010000020         6057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_13340_length_6057_cov_3.102632, whole genome shotgun sequence.
ACCESSION   DAOQWT010000020 DAOQWT010000000
VERSION     DAOQWT010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13340_length_6057_cov_3.102632"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            381..890
                     /locus_tag="PKJ95_00915"
     CDS             381..890
                     /locus_tag="PKJ95_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNR64832.1"
                     /translation="MIPFISTFVLSFFAYLFLTASQGEVLKLWSHYEIFAGIIIALIV
                     AIFASQVLFQKRSYRLLNPYKWVLFLCYFFGPFLHALIKANIDVAYRVITGKINPGIV
                     KISPQLKSDAAITMLANSITLTPGTLSVDIDEDNNDLYIHWINVTDLKPSIEDICGNF
                     PEWVRRIAE"
     gene            887..1162
                     /locus_tag="PKJ95_00920"
     CDS             887..1162
                     /locus_tag="PKJ95_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014013096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNR64833.1"
                     /translation="MTLLLSFFLITAVLITVFILMPLFRLISGPTIPDRVIALDTINT
                     YIVAAMILYGAAFQEIIYIDVAIVYALLSYVATLYIAKYIERRAKDK"
     gene            1159..1521
                     /gene="mnhG"
                     /locus_tag="PKJ95_00925"
     CDS             1159..1521
                     /gene="mnhG"
                     /locus_tag="PKJ95_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HNR64834.1"
                     /translation="MTILTIVLFLFLLIGIFFNGLGSVGLLRFPDVYTRLHATTKCTT
                     FGSIFSSLSVIIFGFYFWKASGDAKFGVLALHTLVALICLIVTNPTGSHAIARAAHRS
                     GVMPKRAVIDELKEANLK"
     gene            1518..1760
                     /locus_tag="PKJ95_00930"
     CDS             1518..1760
                     /locus_tag="PKJ95_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HNR64835.1"
                     /translation="MSPLLSIAHIVVLVIMIVAAILAVVLRDLLSSVIALAAASLLLA
                     LEYYLLHAPDVAIAEAGIGAALTMAIYIFAIRATKK"
     gene            1772..2038
                     /locus_tag="PKJ95_00935"
     CDS             1772..2038
                     /locus_tag="PKJ95_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64836.1"
                     /translation="MKKPVFFIAFIFFLAFMILVAIGMRPFGEPAISNMDDYFIENAQ
                     AETAANNVVTTIVFDYRGFDTLGEGTVLFTAVVGIVALFREVAK"
     gene            2035..2514
                     /locus_tag="PKJ95_00940"
     CDS             2035..2514
                     /locus_tag="PKJ95_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HNR64837.1"
                     /translation="MIGGMSRIVRTMTTLIFGFIMVYGFYVIAHGHLSPGGGFQGGAI
                     VASAFALLLVTYGTSQIQSFLSKDLLSILESTGLVLFITFAFLGLGATFFYNFVSNSG
                     GLFGTPSAIGVNPGDLNTGGTITLMNMAVGLEVLAALGVIVLTMSVASETIHKKEKQ"
     gene            2516..2863
                     /locus_tag="PKJ95_00945"
     CDS             2516..2863
                     /locus_tag="PKJ95_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNR64838.1"
                     /translation="MIGNFPFVTVIILIGLGIYSLLFKKNLIKIAIGLTLIESGVNLF
                     LIALGFRKEAVAPIYTQAPEGVKMVLPTPQALCLTSIVIGVATTALVLSIVMIIYQRY
                     GTINTDEIRRLRG"
     gene            2860..4428
                     /locus_tag="PKJ95_00950"
     CDS             2860..4428
                     /locus_tag="PKJ95_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNR64839.1"
                     /translation="MSSILMHAPALVIALPLLAAFFTPILGRFNRKTISWLASFTLGL
                     TLLLTLFIAKQVLTNGPIVYVFGGTEIGLTLPFGYTMPIRIIFQIDAMGIFMGLITAV
                     VAFLAAIYSISYMEQHTGLDKYYTLLLLLATSMFGMELTGDIFNFFVFFEIASISSVA
                     LVAFRSTISGEPAEAGLKYMVVSSISALMVLFAIGLFYGQYDALNIATIASVMQFTQL
                     DKIALILLVTVLSMKAGSVPMHMWTPDAYSEAPAAITMVLVAASQASLYALFRITFSL
                     YNVTLNTITVGWIIIILGVLSMFIGVTMAIIQSDIKRLMAYHAISQTGYMLLGVGVGL
                     AVLTNPAALESYGIKAMEGGIFHIMNHAMYKGLLFLTAGSLFYRTGTRDLNRMGGLAH
                     NMAYTATFFIIGAAAIAGIPPFNGFASKLIIYESVYQFNPLLSIIAMLVSILTLASFV
                     KVFHSAFLGPQLEHYKSVQEVPKSMVLAMATLACIIVFFGLFPELIVKHIVHPAVMAL
                     IDQARYTATILGGI"
     gene            4430..4771
                     /locus_tag="PKJ95_00955"
     CDS             4430..4771
                     /locus_tag="PKJ95_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNR64840.1"
                     /translation="MHIGLLETGSGYWNALIWVITAVIIGIGVLWLRNKGQENYKKNT
                     DQTKPFLSGNPEVSKEDSHVGASHIYWGFTEALKKYYEPLVKLHTGNINDYSGWIVLM
                     MAIIFIIVGVS"
     gene            4776..5246
                     /locus_tag="PKJ95_00960"
     CDS             4776..5246
                     /locus_tag="PKJ95_00960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNR64841.1"
                     /translation="MKLDKYLDRSLWVFHLNTGSCNGCDIEIVALLTPRYDVERFGIK
                     LVGSPKHADVLLVTGPVNRKMLPRLKLIYEQTPDPKAVIVVGTCGSSGGVFYDSYNIV
                     GPVDKHIPVDVYVPGCPIRPEAIINGVLKAWLKLERLRGSSGVEIEKKIEEVNA"
     gene            5272..5787
                     /locus_tag="PKJ95_00965"
     CDS             5272..5787
                     /locus_tag="PKJ95_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNR64842.1"
                     /translation="MEFFKAKDLLSYLQENLKESILDSRLETRTAGVKKEEFHNIWLE
                     VKREKFRDAVKLLMGLQYPHIAIIAGNDTGEKIELVYLFSLFYGEKFKEISINLKVSL
                     EREKPSIESITDLIPGAQTTEREIKEMFGVDFVGLPDMRNIFLPEDFPKDIFPFRKEQ
                     KGLDELVQKES"
     gene            5799..>6057
                     /locus_tag="PKJ95_00970"
     CDS             5799..>6057
                     /locus_tag="PKJ95_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNR64843.1"
                     /translation="MSVSTYKIPIGPIHPSLAEPMTFNFEISGERIQSVDLAPGDNHR
                     GIEFMGRNRNVVQIIYLAERICGICSASHPFAFCRAVENAAG"
BASE COUNT         1762 a          991 c         1245 g         2059 t
ORIGIN      
        1 tttaaggtat tttatgttat tctgaaaaag tttttacctg agattcagtc agatatgctg
       61 tggctgctaa tatatgtatg agggttctgc attgcgattc caaaggactt tcattatccc
      121 tgtatgccat tattataaaa aagttactgc attaataaat acaaatacaa atataaaata
      181 aaatacactt atttttaata aagaaattgc attatatagc taaaatgtgt tatactcttt
      241 ctggtatttt ggtttgctct ttaacaaata tattttgcat agaagaacta acaaaacaat
      301 atttattcag caaagaattt ctggttgatt tattccagtt aagacaataa ataaatacag
      361 tctcatagag aggtgatgta atgatacctt ttatttccac ttttgttttg agtttttttg
      421 cttatctctt tttaacagcc agccagggag aagttttaaa actgtggagt cattatgaaa
      481 tatttgccgg tataataata gcactgattg ttgctatttt tgcctcccag gtattatttc
      541 aaaaaagatc ttaccgtctc ttaaacccat acaaatgggt tctttttttg tgttattttt
      601 ttggtccctt tttacacgcg ttgattaaag caaatattga tgttgcctac cgggtcatca
      661 ccggtaaaat taatcctggg attgtaaaga tttcaccaca attaaaaagt gatgcagcta
      721 ttactatgct tgctaattct ataactctca cacctggaac attaagcgtg gatatagatg
      781 aggataataa tgatctctat atacactgga ttaatgttac tgatttaaaa ccctcgattg
      841 aagatatttg cggtaatttt ccggaatggg tgaggaggat tgcagaatga ctctattgtt
      901 aagtttcttt cttattacag cagtattaat tacagtcttc attttaatgc cactgtttcg
      961 cttgatctca ggtcctacta taccagaccg tgtcatcgct ctggatacca taaatacata
     1021 tatagtggca gcgatgatac tgtatggtgc agcttttcaa gaaattatct atattgatgt
     1081 ggcgattgtg tatgccttgt tatcatatgt tgctactttg tatattgcta aatatattga
     1141 gaggagggca aaagacaagt gaccatttta acaatagttt tgtttttatt tctattaata
     1201 ggaatatttt ttaatggact cggttcagtt ggtttgttaa gattccctga tgtttacacc
     1261 agactacatg ccaccaccaa atgtacaact tttggttcaa tattctcatc actatctgtg
     1321 attatttttg gtttttattt ttggaaagcc agtggcgatg ccaaattcgg agttttagcc
     1381 ctgcatacct tagtggcttt aatctgcctg attgtaacca accctaccgg ttctcatgct
     1441 attgccaggg cagcccaccg aagtggggtt atgccaaaac gagcggtgat agacgaatta
     1501 aaggaggcta acttaaaatg agtccattat taagcattgc tcatattgtg gtactggtta
     1561 ttatgattgt cgcagcaatt ttagcagtag tattgcgaga tttgttatcc tcagttattg
     1621 ccctggctgc tgcaagttta ctcttagccc tggagtacta tcttctacat gcgcccgatg
     1681 tggcaatagc cgaagcaggg attggagctg ctttaactat ggcaatttat atttttgcca
     1741 ttagggcaac gaaaaagtaa ggagttagat aatgaaaaaa ccagtttttt ttattgcttt
     1801 tatatttttt ctcgctttta tgattttagt tgcaattggt atgaggcctt ttggagaacc
     1861 agctattagt aatatggatg actattttat tgaaaatgca caggctgaaa ctgcagctaa
     1921 taatgttgtt actaccatag tttttgatta tcgtggtttt gataccctgg gtgaggggac
     1981 tgtcttattt acagctgtag taggtattgt ggcattattc agggaggtgg caaaatgatt
     2041 ggaggaatgt ctcgaattgt tagaactatg accactctta tttttggctt tatcatggtt
     2101 tatggatttt atgttattgc acacggacat ctttcaccag ggggtggttt tcagggtgga
     2161 gcaatagtgg catcagcctt tgctctgtta ctggtaactt atggaaccag tcaaattcaa
     2221 agttttttat ctaaagattt gctttctata ctggaaagta cggggttagt tttgtttatt
     2281 acctttgctt ttctaggatt aggggcaact ttcttttata attttgtatc aaacagtgga
     2341 ggattatttg gcacaccatc tgccattggt gtaaacccgg gtgatctgaa cactggcggt
     2401 accatcacac tgatgaatat ggcagtgggt ttagaggtat tggcagccct gggagtaatt
     2461 gttctcacta tgtcagtggc ttctgaaaca atacacaaga aggagaaaca ataaaatgat
     2521 aggaaacttc ccctttgtta cagtaataat tttaattgga ttaggtatct attcccttct
     2581 ttttaagaaa aatttaatta agattgccat tggactgact ttaattgaaa gtggtgtcaa
     2641 tttattttta attgctttag gattcagaaa agaagcagtg gcacctatct atacccaggc
     2701 acctgaagga gtcaaaatgg ttttgcctac tcctcaagcc ctgtgcctga ccagtattgt
     2761 aataggtgta gccaccactg ccctggttct ttctatagtt atgattatct atcaaagata
     2821 tggaaccata aatacagatg aaataaggag gttgagagga tgagcagcat tttgatgcat
     2881 gcacccgctt tagtaatcgc cttacctcta ttagcggctt tttttacccc tatactgggc
     2941 cgtttcaatc gtaaaacaat aagctggtta gcatctttta ccttaggact taccttgctg
     3001 ctaaccctat ttatagccaa acaggtttta accaatgggc ctatagtcta tgtttttggc
     3061 ggaactgaga tagggctgac attgccattt ggatatacta tgcccattag aattattttt
     3121 caaatagatg caatgggaat atttatgggt ttaataactg ccgtggtggc ttttttagct
     3181 gctatatatt caatttccta tatggaacag cataccggtc ttgacaaata ttataccctg
     3241 ttacttttat tggcaaccag tatgtttggt atggaattaa ccggcgatat ctttaatttc
     3301 tttgtttttt ttgaaatagc ctccatttct tctgttgctt tggtagcttt tcgcagcacc
     3361 atttctggtg aacctgccga agcgggattg aaatatatgg tggtgagttc tatatcagct
     3421 ttgatggttt tatttgccat cgggctattc tatggtcaat atgatgcctt aaatatagcg
     3481 actattgcca gtgttatgca atttacccaa cttgataaaa ttgctctgat acttttagta
     3541 actgttttat caatgaaagc cggttcagtc cctatgcaca tgtggacacc tgatgcctat
     3601 tcggaagcac ctgccgcaat taccatggtt ctggttgctg ccagtcaggc cagcctgtat
     3661 gctcttttca gaataacttt ctctctttat aatgtaacct taaatacgat aacagtaggc
     3721 tggataatta ttattttggg tgtactctct atgtttatag gtgtaactat ggctattatt
     3781 caatcagata ttaaacgttt aatggcctat cacgctatct cacagactgg ttatatgctt
     3841 ttaggagttg gggtagggct ggcggtttta acaaatccgg cagcattgga aagttacgga
     3901 ataaaggcaa tggaaggtgg aattttccac attatgaacc atgctatgta taagggttta
     3961 ttgtttttaa cggctggttc tctgttctac agaaccggca ccagagattt aaacaggatg
     4021 ggtggtttag cacataatat ggcttatact gctacctttt ttataatagg tgctgcagcc
     4081 attgctggga tcccgccctt taacgggttt gcttcaaaac taattatcta tgaatcagtc
     4141 tatcagttta atcctctttt atccataatt gctatgctgg taagtatttt aaccttagct
     4201 tcttttgtta aggtttttca tagcgctttt ttaggtcctc aactggaaca ttataaatca
     4261 gttcaggaag ttcccaaaag tatggtgcta gcaatggcta ccttggcttg tatcattgta
     4321 ttttttggct tatttccaga actcatcgtt aaacatattg tccatcctgc agtaatggct
     4381 ttgattgatc aagctcgcta tactgcaaca atattgggag gaatataaga tgcatatagg
     4441 tttattagaa acaggaagcg gttactggaa tgccttaata tgggttataa ctgcagtaat
     4501 aattgggatc ggagtattat ggcttagaaa taaagggcag gaaaattata aaaaaaatac
     4561 cgatcaaact aaaccttttc tatctggtaa tccggaggta agcaaagaag actctcatgt
     4621 tggggcaagt catatctact ggggttttac cgaagcctta aagaagtatt atgaaccact
     4681 ggtaaaactc catactggga atattaatga ttatagtgga tggattgttt tgatgatggc
     4741 aataatcttt attatagttg gggtgagtta ataccatgaa attagataaa tatttagacc
     4801 gttccttgtg ggtattccat ttaaacaccg gttcttgtaa tggttgtgat attgaaattg
     4861 ttgccctctt gacacccaga tatgatgtgg aaagatttgg tattaaactg gtggggagtc
     4921 ctaaacatgc cgatgtgtta ctggtaaccg ggcctgttaa cagaaagatg ctacctcgat
     4981 taaaattgat ttatgaacag acccctgatc ctaaagcggt tatagtggtt ggtacctgtg
     5041 gctcaagcgg tggcgtattt tatgattctt ataatatagt gggacctgta gataaacaca
     5101 ttcctgttga tgtttatgtg cccggttgcc ccataagacc tgaagccata attaacggag
     5161 ttttaaaagc ctggttaaaa ttagagaggt taagaggctc atcaggggta gaaattgaaa
     5221 aaaagattga agaggttaat gcttaaaaaa taagaaggtt aaggtgataa tatggagttt
     5281 tttaaggcca aagatttatt atcatacttg caggaaaatt taaaagaaag tattttagac
     5341 agtcgccttg aaaccagaac agcaggggtt aagaaggaag aatttcacaa tatctggtta
     5401 gaggtgaaaa gagaaaaatt cagggatgct gtgaaactat taatgggact acaatacccc
     5461 catattgcca taattgccgg taatgataca ggggagaaaa ttgaactggt ttatctattt
     5521 tctctttttt atggagagaa atttaaagaa atttcaatta atctcaaagt aagtctggaa
     5581 agagaaaaac catctataga aagtataaca gatttaatcc ctggagcaca aacaacggaa
     5641 agggaaataa aagagatgtt cggagtagat tttgtaggac ttccggatat gcgaaatatc
     5701 tttttaccgg aagattttcc caaagatatt ttcccatttc gaaaagaaca aaaaggcttg
     5761 gacgagctcg ttcaaaagga aagttgaggt gattttaatt gagcgtatct acttataaaa
     5821 tacccattgg acctatccat ccttcgctgg cagagccaat gacttttaat tttgaaatat
     5881 cgggtgagag aattcaatca gtagatttag cgcctggtga taaccatcgg ggaattgaat
     5941 ttatggggcg gaacagaaat gtggtccaaa ttatatatct ggcagaaaga atttgtggaa
     6001 tatgttctgc ttctcaccct tttgcttttt gccgggcagt agagaatgct gctggta
//
LOCUS       DAOQWT010000021         6055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_13344_length_6055_cov_3.177500, whole genome shotgun sequence.
ACCESSION   DAOQWT010000021 DAOQWT010000000
VERSION     DAOQWT010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13344_length_6055_cov_3.177500"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /gene="argS"
                     /locus_tag="PKJ95_00975"
     CDS             <1..1099
                     /gene="argS"
                     /locus_tag="PKJ95_00975"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNR64844.1"
                     /translation="IEFPENGYLGDYIIALAKEVQEKYGTEFKAKDDQETINFFKSYA
                     QQKILQDIKEDLILFGVEFDNWFSEESLYTQDKVYPVIDRLRQEGFVYLEKGALWFKS
                     TDFGDEKDRVVTRANGEAAYFASDIAYHDDKYKRGFQTLIDIWGSDHHGYINRMKAAV
                     QALGYTRDSFQVLLVQFVTLLRAGKTVGMSTRGGQFITLKDLLKEVGKDVARYFFLMY
                     SYDSHTEFDLDIAKSQSLENPVYYIQYAYARICSIIKKGEIKNITLDDINKEQIDLKL
                     LDKREELEIIKKLMSFKKVVERSAIKRKPYLIAGYLLELASLFHKYYTEYKIISRERT
                     LSESRLYLVCSVKIVLENALFLLGIGAPQKM"
     gene            1119..2006
                     /gene="rapZ"
                     /locus_tag="PKJ95_00980"
     CDS             1119..2006
                     /gene="rapZ"
                     /locus_tag="PKJ95_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNR64845.1"
                     /translation="MKDLRFVIISGLSGAGKSVAIKVFEDMEFFCVDNMPPQLIPKFS
                     ELCLKSQGKLSRIAFVVDIRGEIFLRELDRSLKELETMNIYPEILFLEARDEIIVQRF
                     GETRRKHPLQFSHSILENIQEERKKLNELKSKANIIIDTSNLDPKRLNNEIRKYFQKD
                     KSRTMQINLISFGYKYGIPIDVDLLFDTRFLPNPFYVSELSELSGKDEKVKEYLERFS
                     ITRQAIKQFFSLIDFLIPYYIKEGKSYLSIAIGCTGGRHRSVYLANELGQLIRLKDYP
                     VFIRHRDIRKEEKILKEKL"
     gene            2015..3310
                     /locus_tag="PKJ95_00985"
     CDS             2015..3310
                     /locus_tag="PKJ95_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNR64846.1"
                     /translation="MVKKSFHLKSLLKWFYPGMRVKRFLLIILLGIILMAVGLIYLID
                     LTRFLWIKNQIKNLFIYYQIAPHFSGIILILLGSLLIILGISNINRSVLKAFIPKKVD
                     RVPEIIYEQRKLKKGPRIVVIGGGTGLYTLLRGLKQFTSNITAVVTAFDSGGSSGRLR
                     DELGVLPPGDIRNCLVALSTEELLMKKLFQYRFSNGSLQGHSFGNLFITAMSEVSGDF
                     STAIEKSSEILAIRGKVLPSSIDNVTLCAKLKNQELVKGEDKIPLSKDGIESIFIEPS
                     TVLPLPETIRAIQEADTIILGPGSLYTSVICNLLVKDIPEAICQSEAIKVYICNVMTQ
                     PGETDNYTASMHVREIIKYLPYNCLDFILLNSQRLSKQLAARYKKEEAFMVKDDLPSI
                     YDKKTRVLRQELLSEYNFARHHSEKLAKMVMDNIHKEKK"
     gene            3311..4255
                     /gene="whiA"
                     /locus_tag="PKJ95_00990"
     CDS             3311..4255
                     /gene="whiA"
                     /locus_tag="PKJ95_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HNR64847.1"
                     /translation="MYFSPLVKQELARIIPAENSERKSELVAFIIMNGKINSFGELLI
                     TLDNSAMIKVVYFLIKKAFHYETRIDIIRKNDKKKIYYITINDVPMTKLILTHLGLSV
                     NNRGNIIREGTEKEKEKVVIRNFCEKSFLRGAFLAGGFVNDPERMYHLEIACPSKEVA
                     QMINNILHRSSFSTKISFWQKKWTVYIKKSEQIFAFLRFIGVQRALLNLQDIIARKDI
                     LNTVNRLVNCETANLNKTISSASRQLLYIDLILKNIDLANLPPNIKEIVKARSEHPYA
                     SIRELAEIIGGGITKSGVSHRLKKVEQMAEYYLTSNEI"
     gene            4439..5911
                     /gene="rpoN"
                     /locus_tag="PKJ95_00995"
     CDS             4439..5911
                     /gene="rpoN"
                     /locus_tag="PKJ95_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNR64848.1"
                     /translation="MEQNLLMVQKQTLQLSPQMYQSIKILQLNSIELNCWLEKEVQDN
                     PVLEINFNPISDQGTMSDAEKKRDKKDVIGERDVAILDYFSSRKNFIKDQNVSSFEQG
                     PEIRVQKWALGKISLSEYLLLNFGVMARDKLETKIGEYLIGNIDNNGYLAISCQEAAQ
                     DLNIPEKKVRQVLALIQNCSIPGIGARNLKECLLLQLKHIKLPEKKALKKLILFHLDR
                     LSKKNFKDIERELHLSCSEIQHLSDVLRKNFDPKPGRTFFQEYITNFSVPDIIVKKNN
                     DRYEILESNSFFPDIKINSLYERMLLDYKQAEKLKNQELFSQEKWSEIKTTMKYLKGK
                     IDSARWILKCLEQRRNTIINITRFILMYQHDFLEKGVAYLKPLSLEKVAQSLGLNKST
                     ISRAIKSKKIQLPRGIYDLIYFFSIGLAQDNEEVVSNEKIKNLIRNYIERENFYQPYS
                     DQKLAELLQEKEEIKVARRTVAKYRQLMNIPSVKLRRRYK"
     gene            5958..>6055
                     /locus_tag="PKJ95_01000"
     CDS             5958..>6055
                     /locus_tag="PKJ95_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HNR64849.1"
                     /translation="MAIKVGINGFGRIGRNVMRAALNDKDIDFIAV"
BASE COUNT         2147 a          893 c         1190 g         1825 t
ORIGIN      
        1 gattgaattt cctgaaaatg gatatctggg agactatatc attgctctag ctaaagaagt
       61 ccaggaaaaa tatggaacag aatttaaagc taaagacgac caggaaacga ttaatttttt
      121 taaatcttat gctcaacaga aaattttaca ggatatcaaa gaggatttaa ttctctttgg
      181 agtcgagttt gacaactggt ttagtgaaga gtctctttat acccaggata aggtataccc
      241 tgttattgat aggttgaggc aagaaggatt tgtttattta gaaaaaggtg ctttgtggtt
      301 taagtctaca gattttggcg acgaaaagga cagagtggtg accagggcta atggagaagc
      361 tgcttatttt gcttcagata ttgcctacca tgatgataaa tataaaagag gtttccaaac
      421 tctgatagat atatggggct cagaccacca tggctatatt aaccgaatga aggcagctgt
      481 tcaggctctg ggctatacca gagattcttt ccaggtactg ttagtacaat ttgttaccct
      541 gctcagagca ggcaaaactg ttggaatgag caccaggggt gggcaattta taactttaaa
      601 agatttatta aaagaggttg gtaaagatgt tgccagatat tttttcctga tgtatagcta
      661 tgacagtcac accgaatttg atctggatat tgctaaatcc cagtctctgg aaaaccccgt
      721 ctattatatc cagtatgctt atgcccgaat ttgcagtata ataaaaaaag gtgaaattaa
      781 aaatattaca ttagatgata taaataaaga acagatagac ttaaagctgc ttgacaaaag
      841 ggaagagttg gaaattatta aaaaacttat gagttttaaa aaagtagtgg aaagaagcgc
      901 aattaagcgg aaaccatatt taatagcggg gtatttactt gagttagcca gtctgtttca
      961 caaatattat actgaataca aaattatcag ccgagaaaga acactttctg aatcccgttt
     1021 atacctggtt tgttctgtta aaattgtttt agaaaatgct ctttttttat taggtattgg
     1081 ggcaccccag aaaatgtaag tatatttttg aggttagaat gaaagacctt cgctttgtta
     1141 ttattagtgg attatccgga gcgggaaaga gtgttgccat taaagttttt gaagatatgg
     1201 aattcttttg cgttgataat atgccgcctc agctaattcc taaattttct gaactttgcc
     1261 ttaaatcgca ggggaaatta tccaggatag cctttgtggt agatatcagg ggagagatat
     1321 tcttacggga gctggatcgt tcattaaaag aactggaaac gatgaatatt tatcccgaga
     1381 tattattttt agaagctaga gatgagatta tcgtgcaaag atttggggaa acaaggagaa
     1441 aacatccctt gcaattttcc catagtattt tagaaaatat acaggaagaa agaaaaaaat
     1501 taaatgaact aaaatctaaa gcaaatataa tcattgatac ttccaatttg gaccccaaac
     1561 ggctcaataa tgagattcgg aaatatttcc aaaaagacaa gtctcgcact atgcagatta
     1621 atctaatatc ttttgggtat aaatatggca tacccattga tgttgacctg ttatttgata
     1681 ccagattttt acccaaccct ttctatgtta gtgaactgag tgaactatct ggaaaagatg
     1741 agaaagttaa ggagtatttg gaacgatttt ctataacaag acaagccatt aaacaattct
     1801 tttccttaat cgattttctg ataccttatt atataaaaga gggcaagagc tatctatcta
     1861 ttgctattgg ctgtacagga ggaaggcatc gctcagtata tcttgccaat gagcttggcc
     1921 agctgataag attgaaagat tatcctgttt ttataaggca tagggatatt agaaaggaag
     1981 aaaaaatact taaagagaag ttataaaata caagatggtt aaaaaaagtt tccatttaaa
     2041 atcacttttg aagtggtttt atccagggat gagagtaaag cgtttcctgc ttatcatttt
     2101 gctgggaata attcttatgg cagtgggttt aatttacctc atagatctta ccagattctt
     2161 atggataaag aaccagataa agaatctctt tatctactat cagatagccc cgcatttttc
     2221 cggaataatc ttaattttat taggctcttt gctcattatt ttgggcattt ccaatataaa
     2281 tcgttcagtt ttaaaagctt ttatacctaa aaaggttgac cgggtacctg aaattattta
     2341 tgaacagaga aaattaaaaa aaggtcccag aattgtggta attggcggtg ggaccggcct
     2401 ttacacccta ttgagaggtt taaaacaatt caccagtaat attaccgctg tggtaactgc
     2461 ttttgacagt ggaggtagtt caggtagatt aagggatgag ttgggtgttt taccccccgg
     2521 tgatataaga aactgcctgg tggctctttc tactgaagaa ttgttaatga aaaaactatt
     2581 tcagtaccga tttagcaatg gcagtttaca ggggcatagt tttgggaatc tttttataac
     2641 tgctatgtca gaagttagcg gagatttttc tacggcaatt gaaaaatcga gtgagatact
     2701 ggccatcagg ggaaaagtac tcccctcttc tatagacaat gtcactcttt gtgctaaatt
     2761 gaagaatcag gaattggtca aaggtgaaga taagatacct ctgagtaaag atggaattga
     2821 gagtatcttt atagagccct ctactgttct acctttaccg gagacaattc gagctattca
     2881 ggaagcagac actattattc ttggcccggg cagtctttac accagcgtga tatgcaatct
     2941 attggtaaaa gatattccag aagcgatttg tcaaagtgaa gcaatcaagg tttacatctg
     3001 taatgtaatg acccaacccg gggaaacaga taattataca gcgtccatgc atgtaagaga
     3061 gattataaaa tatttaccct acaattgctt ggattttatc ttactaaaca gccaaaggct
     3121 gagtaaacaa ttggcggcaa gatacaaaaa agaggaggct tttatggtaa aagatgattt
     3181 accttctatt tatgataaaa agaccagagt attgagacag gaattattat cagaatacaa
     3241 ttttgcccgc catcattctg aaaagttagc aaaaatggta atggataata ttcacaagga
     3301 gaagaaataa ttgtattttt caccattggt aaagcaggaa ctagcgcgta taataccagc
     3361 tgaaaattct gaaagaaaaa gtgagctagt agcctttatc ataatgaatg gtaaaattaa
     3421 ttcatttggt gaattgttaa tcactcttga taattcggct atgatcaaag tggtttactt
     3481 tttaataaag aaagctttcc actatgaaac caggatagac ataataagga aaaatgacaa
     3541 aaaaaagatt tattacatta ccattaatga tgttccaatg acaaaactaa ttctaactca
     3601 tcttggatta agcgtaaata atcggggtaa tataataaga gagggcactg aaaaagaaaa
     3661 agagaaggtg gtaatcagga atttttgtga aaaatctttt ttacgcggtg cctttttagc
     3721 gggtggtttt gtaaatgatc ctgagaggat gtaccattta gaaattgcct gtccttcaaa
     3781 agaagtagca caaatgataa ataatatctt acatcgctcc tctttttcta ctaaaataag
     3841 tttctggcaa aagaaatgga ctgtatatat aaaaaaaagc gaacaaatct ttgccttctt
     3901 acgttttatt ggagtgcaga gagctctctt gaacctgcag gatattattg cacgaaaaga
     3961 tatcttaaat acggtaaacc gtctggttaa ttgtgaaact gccaacctta acaaaacaat
     4021 aagctcagcc tcccggcagc tactctatat tgatttaata ctaaagaata ttgatctggc
     4081 aaatttgcca cccaatatca aggagattgt caaagcccgt tctgagcacc cttatgccag
     4141 tattcgagag cttgccgaga ttataggagg tggcatcact aagtcaggag tttcccatcg
     4201 cttgaaaaaa gtggaacaaa tggcagagta ctatcttacc tcaaatgaga tttaattggc
     4261 ctgaggaact gatttataac aaatgttatt aagtgattta tcaagctatt tgaaaaaaga
     4321 agataattag tgatagaata tgttgatgtt gataagtatg tttaaaattt cttagaaacc
     4381 atattttaaa caaatagaaa aaatgtttat caataggcag aaggaagagt aaatcataat
     4441 ggaacaaaat ctgttgatgg tacaaaaaca gactttacaa ctatctccac aaatgtatca
     4501 atcaattaaa atattacaat tgaacagtat tgaactgaac tgctggttgg aaaaggaagt
     4561 tcaggataat ccagtcttag agattaattt taacccgatt tccgatcagg gaacaatgtc
     4621 agatgccgag aaaaaaagag ataaaaagga tgtaattgga gaaagagatg ttgctatctt
     4681 agattacttt tccagccgaa aaaatttcat aaaagaccaa aatgtatcat cttttgaaca
     4741 aggccctgaa ataagagtac aaaagtgggc tttgggcaag atttctttat cagaatacct
     4801 tttgttaaat tttggagtaa tggccagaga taaacttgaa actaaaattg gtgagtattt
     4861 gatagggaat attgataaca atggttattt ggcaatcagt tgtcaggagg cagcccagga
     4921 cttaaatatt ccggaaaaaa aagtaagaca ggttctggct ctcattcaga attgttctat
     4981 tcctggtata ggggccagaa atctaaaaga atgcctgctg ctccaattga agcatataaa
     5041 attacctgaa aaaaaagcgc ttaaaaaact aatattgttt catctggacc gattatcaaa
     5101 aaagaatttc aaagatattg agagggagct gcatctttcc tgttctgaaa tacaacattt
     5161 atcagatgtt ctcagaaaaa attttgatcc aaaaccgggc agaacttttt ttcaggagta
     5221 tataacaaac ttttcagtgc ctgatattat tgttaaaaag aataatgacc ggtatgagat
     5281 tttagaaagc aacagctttt tcccggatat taaaataaat tcattatatg agaggatgct
     5341 gttagattat aaacaggctg aaaaactaaa aaaccaggaa ttattttctc aggagaaatg
     5401 gtctgaaatt aaaacaacta tgaaatactt aaagggaaaa attgactctg cccgatggat
     5461 tttaaaatgt ttggagcaaa gaagaaatac aattattaac ataaccagat ttatacttat
     5521 gtatcagcat gattttctgg aaaaaggagt ggcttatcta aagccattat cattagagaa
     5581 ggttgcccag tctttaggtt taaataaatc caccatttcc cgggctatca aatcaaagaa
     5641 gattcagtta cccagaggta tttatgattt gatatatttt ttttctatag gattagctca
     5701 ggataatgaa gaggtagttt ctaatgaaaa aatcaaaaat ttaatcagaa attatattga
     5761 aagagaaaat ttttatcaac cctattccga tcagaaatta gccgaattac tacaggaaaa
     5821 ggaagagata aaggttgcgc gaaggaccgt ggcaaagtat cgtcaattga tgaatatacc
     5881 ttctgtaaaa ttacgtagaa gatataaatg aaaatagaaa aatttaaaat tgtaattatt
     5941 tcaaaatagg aggaaatatg gctattaaag ttggaattaa tggatttggc agaattggca
     6001 gaaatgtaat gcgggctgca ctgaatgata aggacattga ttttattgcc gttaa
//
LOCUS       DAOQWT010000022         5947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_13667_length_5947_cov_3.274100, whole genome shotgun sequence.
ACCESSION   DAOQWT010000022 DAOQWT010000000
VERSION     DAOQWT010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13667_length_5947_cov_3.274100"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="PKJ95_01005"
     CDS             complement(<1..1512)
                     /locus_tag="PKJ95_01005"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HNR64850.1"
                     /translation="MKIETKKVYVFFILLTLIFSLMVGIKRSVLENSYKQIDIVMSLN
                     KIRELSIKDGKDENEILRQAKLKGITGIAIQEDTITTLLLQGKIAFFSPNDLSKFNYF
                     YHLDLAQFDNISNNGFMIACQDYDLFQRIKENIQSYLGRDFIKTSTIDGKYYLRILQG
                     DEEELLKLGLGYSKEDILKIQQMAFNLILRPKNTSKINQEIFQQKLQSIEDFDDIALI
                     IFDEEEVLGYPSLKMLTGMANFLEKKNYSFGIIEFTSQKGIQTIASKISELAVRVHSI
                     TKEEMEKIDQNKAIERWVRAAQERNIRLFYLNPFLNIREGSLVESNLDYIEKIKDELI
                     KNNYQIGEASLFPTYQNPLIVVFIIGFGIISAGILLLLEFVNIDKKYEIMLFLSILLF
                     LLLVNTAIGTIFLMKILALASALIFPSLAIIINKKYFIKVASTKNNSIVITDKPFFLY
                     TKIIKNIFVGITRIMLISLLGGLTIAALLTQRQFILAIQLFSGIKVAYVFPIFI"
     gene            complement(1804..2922)
                     /locus_tag="PKJ95_01010"
     CDS             complement(1804..2922)
                     /locus_tag="PKJ95_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNR64851.1"
                     /translation="MNIVHSLIELVEISGFVGLNLPSLIMLLVGGTLLYLAIVKNYEP
                     LLLLPIAFGAILVNLPFTGLMEEGGFLNLLSFGIRHELFPILIFMGIGAMTDFGPLLA
                     NPVTFLLGATAQLGVFVAIIGAVLIGFNIKEASAIGIIGGADGPTAIYLAAKTAPHIL
                     GAIAVAAYSYMSLVPLIQPPIMKLCTTKEQRKTVMKQLHPVSKTEKILFPIICTIFVG
                     LLLPATVPIVGIMMLGNLFRESMVVDRLSNTTQNELINIVTIFLGTCVGATMEAKYFL
                     TVETIKIITLGLIAFGFSTFGGVMFGQVLYRLSGGKINPLIGAAGVSAVPMAARVAQR
                     VGQQANPRNFLLMHAMGPNVAGVIGTAVAAGVILTLLT"
     gene            complement(2946..3344)
                     /locus_tag="PKJ95_01015"
     CDS             complement(2946..3344)
                     /locus_tag="PKJ95_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNR64852.1"
                     /translation="MKQFKIKVNGKEYLVEVEEIGTGNTPPTATPVVKTEAKEVKVET
                     APVKPEVKKEASVTVSEGEEVIVAPMPGKILKINVSEGDTVQEGDTIVILEAMKMENE
                     IVAGSGGKVKKIHVAVNQMVETDDALAVIG"
     gene            complement(3348..3755)
                     /locus_tag="PKJ95_01020"
     CDS             complement(3348..3755)
                     /locus_tag="PKJ95_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HNR64853.1"
                     /translation="MYEGMQGAIVLSIISMVIVFIVLGLLALLMVGLRKFMAFTSKKP
                     AKDEKGIVKGEKAAPVPKETTGVEETANEEIVGVISAAVASYLHSTTSALPLSIISIK
                     RIIPQTLNYWAISGRQNMMTERISISSRKKGGF"
     gene            complement(3826..5382)
                     /locus_tag="PKJ95_01025"
     CDS             complement(3826..5382)
                     /locus_tag="PKJ95_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNR64854.1"
                     /translation="MEQSINQLMKVLQEKKEKILAGGGEKRVTAQHERGKLTARERIS
                     LLLDEGSFNELDLFVEHRCTNFDMAGKEVPGEGVVTGYGTVNGRLVYVFSQDFTVIGG
                     SLGEMHSKKICKVMDMAMKMGAPVIGINDSGGARIQEGVDSLSGYGQIFYRNTIASGV
                     VPQISVVVGPAAGGAVYSPAIMDFVFMVKKIGIMHITGPSVIKAVTGEDVSSEKLGGA
                     MTHNQKSGVAHFAAENEQEVFKSVRKILSYMPSNNMENPPVEPTNDVSNRMEEALLNI
                     VPTDPNKPYEMRDIIKYIVDNGDFFEAHEHFARNILTGFARLNGQSIGIIANQPKILA
                     GCLDIDASDKAARFIRFCDAFNIPILTLVDVPGFLPGTNQEYGGIIRHGAKMLYAYSE
                     ATVPKVTVVVRKSYGGAYLAMCSRDLGADQVIAWPTAEIAVMGSQGAANIIFKKEIAE
                     AENPEQYRQKKIDEYQEQFSNPYAAAARGYVDMIIDPRETRPRLITTFEMLSTKRESL
                     PGKKHGNIPL"
     gene            complement(5509..5925)
                     /gene="mce"
                     /locus_tag="PKJ95_01030"
     CDS             complement(5509..5925)
                     /gene="mce"
                     /locus_tag="PKJ95_01030"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNR64855.1"
                     /translation="MVLLKKINHIGIAVKNLNDALSVFQDILGMTCSGIEEVEDQLVK
                     VACLPVGESKIELLESTSPEGNIARFIEKKGEGIHHIAFEVGDLEGVIEDLKRKGVQL
                     IDQKPRIGAGGIKIAFLHPKSTKGVLVELCEHKDQD"
BASE COUNT         1803 a         1228 c          883 g         2033 t
ORIGIN      
        1 aataaaaatt ggaaatacat atgcaacttt tattccacta aacaattgta tggctaatat
       61 aaattgccgc tgggttaaca acgctgcaat ggttaatcca ccaagcaatg aaatcaacat
      121 aatcctggtg atacctacaa aaatattctt aataattttt gtatataaga aaaatggttt
      181 atcagttata acaatggaat tattttttgt tgaagccacc ttaataaaat attttttatt
      241 tattattatc gctaaacttg gaaaaattaa tgcacttgcc aatgccagta ttttcattaa
      301 gaatattgtg cctattgctg tattaacaag cagtaagaat aaaagaatac ttaaaaatag
      361 cataatctca tattttttat caatattaac aaattccaat aatagcagta ttccggcaga
      421 aattatacca aatccaatta tgaaaacaac aattaagggg ttttgatagg tggggaatag
      481 gcttgcttca ccaatttgat aattattttt gataagttca tcctttatct tctcaatata
      541 atcaagattg ctttctacta aacttccttc tcttatgttt aagaatgggt ttaaataaaa
      601 gagccttata tttctttctt gtgctgcccg tacccatcgc tctattgctt tattttggtc
      661 aattttctcc atctcttctt tggttatact atgtacgcgc acggccaatt cactaatctt
      721 agaagcaata gtctgaattc ctttttgtga agtaaattcg ataattccaa aagaatagtt
      781 tttcttttct aaaaaatttg ccatccctgt aagcattttt aatgaagggt aaccaagtac
      841 ttcttcttca tcaaatataa tcagagcaat atcatcaaaa tcttctattg attgtaattt
      901 ttgttggaaa atttcctgat tgattttaga agtattttta ggtcttaaaa tgagattgaa
      961 tgccatttgt tgaattttta aaatatcttc tttagaatat cccaaaccca attttaataa
     1021 ttcttcttca tcaccttgca atattcgtaa ataatatttt ccatcaatgg tactggtttt
     1081 tataaaatct cttcctaaat aggattggat attttctttt atcctttgaa ataagtcata
     1141 atcctggcat gctatcataa aaccgttatt ggaaatattg tcgaattggg ctaaatcaag
     1201 atgataaaaa taattgaact tgctcaagtc attcggggaa aaaaaggcaa ttttaccttg
     1261 cagcaataac gtcgtaatag tatcttcctg aattgctata ccggttattc cttttaactt
     1321 agcctgtctt agtatttcat tttcatcttt tccatcttta atggatagct ctcttatttt
     1381 gtttaaactc attactatat caatctgttt gtagctgttt tccaaaactg acctttttat
     1441 tcccaccatc agggaaaaaa taagagttaa taaaataaaa aaaacataca cttttttggt
     1501 ctctattttc aaaatattta cctcataata tcattaaaag attggtaata ttttaacata
     1561 ccatcagata attgtaaaaa tgcacctcta tcaaaatgta ataaaggtgc atttttataa
     1621 taataattac cattaatata ataatttcaa atagcctgtt aaaagtagtc ttaaagttat
     1681 gattttaaat tattttcctt actatcaaat caaatccttt gctattttta gaatgctatt
     1741 ctaacagatt attttctatt aatattattt aaaataacag attaatgaaa tatttgttgt
     1801 tctttatgtt aataaggtca gtataacccc tgctgccact gctgtaccaa ttacccctgc
     1861 tacattggga cccatggcat gcattaataa gaaattcctg gggttggcct gctggcctac
     1921 cctttgggct actctggctg ccatcggtac tgcagatact cctgcagctc caattagagg
     1981 gttaatcttt ccacctgata gtcggtataa tacctgtccg aacattaccc cgccaaaggt
     2041 actgaagcca aaagcaatca atcctagggt tattattttt atagtctcca ctgtcaggaa
     2101 atattttgcc tccatggtgg caccaacaca ggttcccaga aaaattgtca caatattgat
     2161 aagctcattt tgggtggtgt tggatagtct atctactacc atactctccc tgaacaggtt
     2221 ccccagcatc ataataccta caataggcac agtagctggt aataatagtc ctacaaatat
     2281 tgtacaaata atggggaaaa gtattttctc ggttttagaa acaggatgta gctgcttcat
     2341 tactgtcttt ctctgttcct tggtagtaca caacttcatt attgggggtt ggattaaggg
     2401 tactaaagac atataggaat aagcggctac tgcaatcgct cctaagatat gcggtgctgt
     2461 cttggcagcc aaatagattg ccgtaggtcc atcggcgcct cctatgatgc ctattgctga
     2521 agcttcttta atattgaaac ctattaatac tgctcctatt atggccacaa aaacgccaag
     2581 ctgggcagtt gctcctaata aaaaagttac cggattggcc aacagtggtc caaagtcagt
     2641 catagctcct atccccataa agattaagat gggaaatagt tcgtggcgta ttccaaaaga
     2701 gagcagattt aaaaatcctc cttcttccat taaaccggta aaaggtaaat ttactaaaat
     2761 tgcaccaaag gcaattggta acaacaataa tggttcataa ttttttacta tggccagata
     2821 taacagggtt ccccctacta acagcattat taaacttggc aggtttaagc cgacaaagcc
     2881 ggatatttct acaagttcga ttagggaatg aactatattc atatcatact ccttttttga
     2941 tattcttaac ctattacggc gagggcatca tcggtttcca ccatctgatt cacggcaaca
     3001 tgtatctttt taactttgcc gccagaacca gcaacaattt cattctccat cttcattgct
     3061 tccagaatca cgatagtatc accctcttga actgtatccc cttctgatac atttatttta
     3121 agaatcttac cgggcatggg agctacgata acctcctcac cttctgagac ggtaacagag
     3181 gcttcctttt ttacctcagg ttttactggt gcagtctcaa cttttacttc ctttgcttct
     3241 gtcttgacaa ctggagtagc agtaggaggg gtattgcctg tgcctatctc ttccacctct
     3301 accagatatt ctttaccatt tactttgatt ttaaattgtt tcatttacta aaatcctcct
     3361 ttttttctgg aactaattga tattctttct gtcatcatat tttgtctgcc cgaaattgcc
     3421 cagtagttta aagtctgtgg tataatcctt tttatactaa taatactgag tggtagtgct
     3481 gatgttgtac tatgtagata actagcaaca gctgccgata taacaccgac tatttcctca
     3541 tttgccgttt cttcaacacc tgttgtttct ttcggtactg gagccgcttt ctctcctttg
     3601 acaatccctt tttcatcttt ggctggtttt tttgaagtga aagccatgaa ttttctcagt
     3661 cctaccatca ataatgccag caaacccaat actataaata caataaccat tgaaattatt
     3721 gataatacaa ttgcaccctg cattccttca tacatattat gcaccttctt tcaataggta
     3781 aaatatattc agataacact tattaaaagt aattttccac tttatctaaa gagggatatt
     3841 tccatgcttt ttacctggta aactttctct tttagtagaa agcatttcaa aagttgtaat
     3901 caatctcggt ctggtctcac ggggatcaat aatcatatct acatagcctc tggctgctgc
     3961 ggcataagga ttagaaaatt gttcttgata ttcatcaatc tttttctgcc ggtactgttc
     4021 agggttttct gcttctgcta tttctttctt aaagattata ttggcagcac cttgagaacc
     4081 cattacggca atctctgctg taggccaggc aatgacctgg tctgctccta aatccctact
     4141 gcacatagcc agatacgctc caccatatga ttttcttacc accactgtaa ccttgggaac
     4201 ggtcgcttca gaataggcat ataacatctt tgccccatgt ctgataattc ctccatactc
     4261 ttgattcgtt cctggtaaaa atcctggaac atctactaga gttaagatag gaatattaaa
     4321 ggcatcgcaa aaacggataa aacgagcagc tttatcagag gcgtcaatat ccagacaacc
     4381 agcaagtatc tttggctgat tggcaataat cccgatactc tgaccattta atctggcgaa
     4441 cccggtgaga atattacgag caaaatgttc atgtgcctca aaaaaatcac cattatcaac
     4501 aatatatttt ataatatctc tcatttcata gggcttatta ggatctgttg gcacaatgtt
     4561 taataatgcc tcttccattc tattggaaac atcattggta ggttcaacag gaggattctc
     4621 catattattg gaaggcatgt aactaagtat tttccggact gatttaaaaa cttcctgttc
     4681 attttcagct gcaaagtggg caactccact tttttggtta tgagtcattg ctcctcctaa
     4741 tttttctgag gagacatctt cacctgtaac tgctttaata acactgggac cggtaatatg
     4801 cataatgcca atcttcttaa ccataaagac aaaatccatt atcgcaggag agtatacagc
     4861 tccgcctgca gctggaccaa ctactacaga aatctgggga accacacctg aggcaatagt
     4921 attccgataa aatatttgtc cataaccact caaagagtct acgccttctt gaatacgagc
     4981 accaccagaa tcattgattc caataacagg tgcacccatt ttcatagcca tgtccataac
     5041 tttgcatatc tttttagagt gcatttctcc taaagaacca ccaatgacag taaaatcctg
     5101 ggaaaaaaca taaaccagac gtccgttaac tgttccataa ccggtaacca ctccttctcc
     5161 tggcacttcc ttcccagcca tatcaaaatt tgtacaacga tgttccacaa aaagatctag
     5221 ctcattaaaa ctcccctcat ctaataataa ggatattctt tcacgggcgg tcagttttcc
     5281 tctttcatgt tgtgctgtaa ctcttttttc tcctccgcca gcaagaatct tttctttctt
     5341 ttcctgcaaa actttcatta attgattaat agattgttcc attagacact ccaccttcct
     5401 ttaattattt aatccttata gaagtttaat tgaatctttc taaaaaaata aatatataat
     5461 taaatcttat aaataataag tccttcaact tttttagaat taatacttct aatcctgatc
     5521 cttgtgctcg cataattcga ctaaaacccc cttagtgctc ttaggatgta gaaaagcaat
     5581 tttgatgcct cccgcaccaa ttcgtggttt ctgatcgatt aattgaaccc cttttctctt
     5641 gagatcttct atcacccctt ctaaatcacc aacttcaaag gcaatatgat gaatgccttc
     5701 cccttttttt tcaataaacc tggcgatgtt tccctctgga gatgttgatt ctaataattc
     5761 aattttgctt tcaccaacag gcagacaggc tactttaaca agctgatctt ctacttcttc
     5821 tataccagaa caggtcatac ccagaatatc ctgaaaaaca gaaagagcat catttagatt
     5881 ttttaccgca atcccaatat ggtttatttt ttttaacaac accatctccc cctttaaaaa
     5941 ttttttc
//
LOCUS       DAOQWT010000023         5940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_13684_length_5940_cov_3.488190, whole genome shotgun sequence.
ACCESSION   DAOQWT010000023 DAOQWT010000000
VERSION     DAOQWT010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13684_length_5940_cov_3.488190"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKJ95_01035"
     CDS             complement(<1..189)
                     /locus_tag="PKJ95_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNR64856.1"
                     /translation="MNREDNIIKGKSIADAIGEELKLDSAQFFQKTAMKPKLSVVIVG
                     DDPAALFYVRMIEKSCQKV"
     gene            complement(422..2380)
                     /locus_tag="PKJ95_01040"
     CDS             complement(422..2380)
                     /locus_tag="PKJ95_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNR64857.1"
                     /translation="MDCQKEAFICKTGLIPLLLLTILFLTSFTFVALAQDYIDYEIQK
                     GDCLWSIARQFQLSIQEIAQTNNIDTKETLHPGLLLKIPQSESGSLITAESAQMIIHT
                     VKKGESLWEIAQQYHLSLEQLSKANELKQPNSLYIGQEVKIPISNSNNNDICISDNDS
                     TGTDSQAILSFQEEKASLKEISTELNLEFKESVNEIEYVVKPGENLWTIAQNYQVSLK
                     ELSQANGLENEERLTIGQIIKIPLWEKSSDNKEKAGQKEEKEPEGSYIEHIVLYGETI
                     STISQKYHTPVETICQLNQITTRDYIYPGQRLKVKVNGQIPPELAQLPGEEKKENSIQ
                     ADKEIAETETVYYTVKPGDTLCGIAQKYAVSVEGIVAVNYLINKDKLSVGQRLQIPAI
                     GGKNIKTSVVEYTVAKGDTLSSIAQKFNIRMYDIMSLNDLENVNRLSVGQKLNIPASA
                     TAADQGVSSTSVSQAAERKDVVYYVQKGDTLWRIAQQYQVSLQSITSTNGISENSRLS
                     VGQKLVIPNARGSSGSSTSLSFAWPIKGLITSHFGNRTFGGRRDYHTGIDIDGRTGDS
                     VRAAESGKVSFSGRISGYGNIIIIDHPGGYSTVYAHNSANLVQKGQSVSKGEVIARLG
                     ATGNATGSHLHFEVRENSKPVNPLNYLP"
     gene            complement(2639..2821)
                     /locus_tag="PKJ95_01045"
     CDS             complement(2639..2821)
                     /locus_tag="PKJ95_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR64858.1"
                     /translation="MEEYLTAKQVAEYLQVKPLTIYQWAREDKIPAIKIGRIWRFKKE
                     AIDTYLEEQLHQKGSK"
     gene            complement(3052..5343)
                     /locus_tag="PKJ95_01050"
     CDS             complement(3052..5343)
                     /locus_tag="PKJ95_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNR64859.1"
                     /translation="MNNFHNQLKKILFIFSLLLVVLILSSTIFAKQEDEAARAEKQLK
                     IFQLSYLLEKDGGFSVQKAYFNNALAYLENGYSSMAITELEKIEYNNLYIPFYLKSQL
                     LSAQCYENLKRWESAIRIYEDLLTLVPVIQEYVIYLLARAYQNIKDINNAIKSYSEII
                     AQYPQSALSPLVHYQLALLYQESNQEELFWQECYLAVETSAEEKFKAKVFIKMCDTLW
                     EEGEYLDSLNYLREIIENRYERERISWQENLYINRFQEVKENMLLEIPPDLSLFFAET
                     IFNYGRYKMAEMVYQEMIEKYAGQIDLPRIHYHKARAIYYQGEYQRAIDECLYILDNF
                     KDTDNQEDVIVRTLYLYAGALLSTGNRSWAIEKYREIIEKFPEHFFAQLSYFRQSEIG
                     FLENKTEEGILYLKKLLLDFPESSLAQEAAWELSRYYTNQNSVSEALYYYQFIYEHFP
                     QGNQADDALYWLGKLLYPTDKAEGMKWYERLLTQFPDSYYSFRIPIEMKSDEHNLENI
                     IEQCKTITPDSFKQSHFPQDKRAQLATYRAELLMFLKLYQESVQEIKNALKKESENLY
                     LQLLLMQVYAEAGEYYQSVSCAQAILDYLLSANNREFPFMVWQYAFPIFYEEYIKQTA
                     SSYQVDPYLVWSIMREESHFNPYAESRAGARGLMQIIFSTGEWIAQKLNYKEFEYDSL
                     FEPELNINLGNWYLKYLQERFNMNTFLIISGYNAGPGITDKWVETTDMSDIDVFIENI
                     PYKETAEHIKKVMRSYLIYKIIY"
     gene            complement(5381..>5940)
                     /locus_tag="PKJ95_01055"
     CDS             complement(5381..>5940)
                     /locus_tag="PKJ95_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR64860.1"
                     /translation="IAPGIVAVDECNYEYLGESVVDLIEQYPNLAISRSFSKNFGLAG
                     LRLGFAVSSSKNIVEIARYGQHFRVNKMAERAGIKVLKYLDYFQNIWQEIARTRDFFI
                     AGLKGLNIMVLPSKTNFVLVDFTTEEKARKIWNFLRGEKVFTVAGWEQEFSGLDNQYL
                     RFTISNQEEMSFVLELLKRFPENTS"
BASE COUNT         1764 a         1170 c          984 g         2022 t
ORIGIN      
        1 gaccttctgg caactctttt caatcatacg aacgtaaaac aaagcagcag ggtcatcacc
       61 tacaataacc accgaaagtt ttggtttcat agctgttttt tggaagaatt gagcagaatc
      121 taacttcaat tcctcaccaa tagcatcagc aatacttttt cctttgatta tgttgtcttc
      181 cctattcatt atcataactc cctttttttg ttatttttta tctttcttct gaaattgccc
      241 ttaatatctt catttaaatt ttagattttt ctgtgaagca tgatttaata caattataaa
      301 ataagtgctg atcttccgca aaaatatttt ttaaattaaa actgtagaga taatagttgt
      361 aataattaga taattgataa aaaattttac actgttctca tgtaaaatat ttatctaaaa
      421 tctacggtaa gtagtttaag gggttaaccg gtttgctatt ttccctgact tcaaaatgga
      481 ggtgtgaacc tgtagcattg cctgtggcac caagtcgggc aataacttca cccttactga
      541 cactttgccc tttttgaact aaattggcag aattatgagc ataaacagta gaatatcctc
      601 ccggatgatc aattatgata atgttaccat atccactgat acgtccagaa aaactaactt
      661 taccgctttc ggcggctctg acagaatccc ctgttcgacc atctatatca ataccggtat
      721 gataatcacg ccttccgcca aaagttctgt tcccaaaatg tgaagtaatc aaacccttta
      781 ttggccaggc aaaagaaaga gaggtgctgg agccggaact accgcgagca ttgggtataa
      841 ccagtttctg ccccacggat aatctgctgt tttcagaaat gccattcgta ctggtgatgg
      901 attgcaggct aacctggtat tgctgtgcaa tacgccagag agtgtcacct ttctggacat
      961 aatatacgac atcctttctc tcagctgcct gtgaaacaga ggtactgcta acaccctgat
     1021 cagcagcggt ggcagaagca ggtatgttta acttttgacc aacagagagt ctgttcacat
     1081 tttctaaatc attcaaactc ataatatcgt acattcttat gttaaacttc tgggcaatgc
     1141 tggatagagt gtctcccttg gcaacggtat attcaaccac cgatgttttt atattttttc
     1201 cacctatagc cggtatctgc aacctctgac ccacagataa tttatcttta ttgatcaggt
     1261 agttaaccgc cacaattccc tctaccgaaa cagcatattt ttgggcaata ccacataagg
     1321 tatcccccgg tttaacagta tagtaaacag tctctgtttc tgctatttct ttatcagctt
     1381 gaatactatt ttctttcttt tcttctcccg ggagttgtgc taattcagga ggaatctgcc
     1441 catttacttt aactttaagt ctctgaccgg ggtaaatata atctctggta gtaatctggt
     1501 taagctgaca gattgtttca acaggagtat ggtatttctg ggaaatagta gagatagtct
     1561 ccccatataa tacaatatgt tctatatagc tgccctcagg ttctttctcc tctttctggc
     1621 ctgctttttc tttattatcg ctgctttttt cccacaaagg tattttaata atctgaccaa
     1681 tagttaatct ttcttcattc tccaaaccat tggcttgaga aagttctttc agggaaacct
     1741 ggtaattttg agcaatagtc cagaggtttt ctcctggttt taccacatat tcaatttcat
     1801 tgactgattc tttgaattca agatttaatt cggtgctaat ttctttcaga ctggctttct
     1861 cttcctggaa agataatatg gcctgactat cagtacctgt agaatcatta tcactgatac
     1921 atatatcatt attgttgcta ttactaatgg gaattttaac ctcctgtcca atatacaatg
     1981 agttgggttg ttttaattca tttgctttgg acagctgttc caatgacagg tggtattgtt
     2041 gggcaatctc ccagagactc tctccttttt taacagtatg tatgatcatt tgagctgatt
     2101 ctgccgtaat aagggaacct gattcacttt ggggaatttt caagagaagc ccagggtgca
     2161 gggtttcttt ggtatcaata ttgtttgttt gggcaatttc ctgtatggat aactgaaatt
     2221 gccgggcaat tgaccagagg caatcacctt tttgtatctc gtaatctata taatcctgag
     2281 ctaaagcgac aaaagtaaaa ctggttagaa aaagaatggt tagcaataac aatggtatta
     2341 gcccagtttt acagataaaa gcttcttttt ggcaatccat ccttctataa cacctaataa
     2401 attaaattta attattgtat ctccgactgg gttacaagaa gagatattct atttttaagt
     2461 tgggtaagtc aaaataagga agaattttat ctttttacca ttatattata ttctttgaaa
     2521 aaaatgcaag aaatttagaa attttattta tagtacatta atatatagag agctatacca
     2581 gacataacgt ctctaaatac agcaccccga gagagaatct ttcaggtaga ctgttctatt
     2641 atttacttcc tttctggtgc aattgttctt ccagataggt atcaatagct tcttttttaa
     2701 aacgccatat tcttccgatc tttatagctg gtattttatc ctctcttgcc cattggtaga
     2761 tggtcagagg tttaacctgc agatattctg ctacttgttt tgcagttaga tattcttcca
     2821 ttatcttctc ctctctacaa tagaaaaagt tttagtatct cttagcgcta aatcagtgtc
     2881 cttataggta ataaagtcca aggtccgtat gctcatctct ctcactttat cctggactgt
     2941 tttattataa agacctgaaa aaataaaaat atttaattat tattgtctat tctatggttt
     3001 ttttatggta ttgtcaaatt taggtctatc tttatcttag tttttgtaat tttaataaat
     3061 aattttatag ataagatagg aacgcataac ttttttaata tgttcggctg tctctttata
     3121 gggaatattt tcaataaaaa catctatatc gctcatatcg gttgtttcta cccacttatc
     3181 agtaatgccc ggaccagcat tataccccga aataatcaaa aaagtgttca tattaaacct
     3241 ttcctgtaaa tattttaaat accagtttcc taaattaata ttaagttccg gttcaaagag
     3301 tgaatcatat tcaaactcct tataattgag tttttgagca atccattcac cggtagagaa
     3361 aatgatctgc attaaccccc tggctcctgc ccttgattcg gcataaggat taaaatgact
     3421 ctcttctctc ataatggacc aaacaagata gggatccacc tggtatgaag aggcagtttg
     3481 ttttatatat tcctcataga aaataggaaa ggcatattgc cacaccataa aaggaaactc
     3541 tctgttatta gcagacaata gataatcaag tatagcctgg gcacaactga ccgactgata
     3601 atattctccg gcttcagcat agacctgcat aagtagaagt tgtaagtaga ggttttcaga
     3661 ttcctttttt aaggcatttt ttatttcctg aacagattct tgatataatt taaggaacat
     3721 tagtagctct gcccgatatg ttgctaactg ggctctttta tcctgaggga aatggctctg
     3781 tttaaaacta tcaggggtaa ttgttttaca ttgttcaata atattctcca gattatgttc
     3841 atcgcttttc atttctatcg gaattctaaa ggaataatag ctatctggga attgggttaa
     3901 taaacgttca taccatttca tcccttccgc cttatccgtg ggatataaca gtttccccag
     3961 ccagtataag gcatcatctg cctgattgcc ttggggaaaa tgctcataaa taaattgata
     4021 atagtacaat gcctcagaaa cagaattctg attggtataa taacgggaca attcccaggc
     4081 tgcctcttgc gccagcgaag attccggaaa atcaaggagc agcttcttta aatacaagat
     4141 cccttcctcc gtcttattct ccaaaaagcc aatttctgac tgacgaaaat aggacaattg
     4201 ggcaaaaaaa tgctctggaa acttttctat tatctctcta tacttttcaa tagcccagct
     4261 gcgattcccg gtagagagta gtgcaccggc atagagatat aaagttctta ctattacatc
     4321 ttcctggtta tcagtatctt taaagttatc taagatataa agacactcat ctattgccct
     4381 ctggtactca ccctgataat aaatagccct ggctttatga taatgtattc ggggtagatc
     4441 aatctgcccg gcatatttct caatcatttc ctgataaacc atttctgcca ttttatatcg
     4501 gccataatta aatatagttt cagcaaaaaa taaggacaaa tctggcggta tctcaagcag
     4561 catattctct tttacctctt gaaatctatt tatatataaa ttttcctgcc aggaaatcct
     4621 ttctcgttca taacgatttt ctatgatttc cctcagatag ttcaatgaat cgagatattc
     4681 cccttcttcc cataaagtat cacacatttt aataaaaact tttgctttaa acttttcttc
     4741 tgctgaagtc tccaccgcca aataacattc ctgccagaat agctcctcct gattggattc
     4801 ctgatagagc aatgccagct ggtagtgtac cagaggactc aaggcacttt gggggtattg
     4861 tgcgataatc tctgaatacg attttatagc attattgatg tcctttatat tctgataggc
     4921 tcgtgccaac aaataaatta catattcctg tataacgggc accagggtaa gaaggtcctc
     4981 atagattctt attgctgatt cccagcgttt taagttttca tagcattggg cgcttaataa
     5041 ctggcttttt aaatagaagg gaatatacag gttgttatac tcaatttttt ctaattctgt
     5101 gatagccata ctggaatacc cgttttccag ataagcaagg gcattgttaa aataggcctt
     5161 ctggactgaa aatccgccgt ctttttctaa aagataagaa agctgaaaaa ttttaagctg
     5221 cttctcagct cttgcagcct catcctcctg cttggcaaat atagttgaag aaagtattaa
     5281 tacaaccagt aatagggaaa aaataaataa gatttttttt agctgattgt gaaaattgtt
     5341 caattagcgg aagtctccct aaaaattaaa aaaaattatt ctaagaagta ttttctggaa
     5401 atcttttcag tagttctaag acaaaggaca tttcttcctg gttacttatg gtaaaacgaa
     5461 ggtattggtt atcgagacct gagaattcct gctcccagcc ggcaacggta aagactttct
     5521 ctcctcttaa aaaattccat atctttctgg ctttttcctc agtagtaaaa tcaactaaaa
     5581 caaaatttgt tttagagggt aagaccatta tattcagacc ttttagtccg gcaataaaga
     5641 agtctcttgt ccgggcaatt tcctgccata tattttgaaa ataatccaaa tattttaata
     5701 ccttaatacc tgccctctct gccattttat ttactctaaa gtgttgtcca tagcgggcaa
     5761 tttcaacaat gtttttggat gaagaaacag caaagccaag tcttaaccct gccagaccaa
     5821 aattctttga aaaactccgg gaaattgcta aatttggata ttgctcaatt aaatccacca
     5881 ctgattcacc taagtattca taattgcact catctaccgc cacaatccct ggtgcaatat
//
LOCUS       DAOQWT010000024         5807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_14108_length_5807_cov_3.336057, whole genome shotgun sequence.
ACCESSION   DAOQWT010000024 DAOQWT010000000
VERSION     DAOQWT010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14108_length_5807_cov_3.336057"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>735
                     /locus_tag="PKJ95_01060"
     CDS             <1..>735
                     /locus_tag="PKJ95_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNR64861.1"
                     /translation="RIAGVDLIVDDTPEAVIISGFDPIRREIARIALEKLITDGRIQP
                     ARIEEVIESSKKIVENNIREEGEKAVFETNVRNLNPKLINILGKLKYRTSYGQNVLQH
                     SKEVAYFAGMMADELGLNSSLSRRAGLLHDIGKSVNMEVEGPHALIGADIARKNGESP
                     KVVNAIAAHHEDEKPESAEAILIQAADAISASRPGARREMLETYIKRLQQLEEIANSF
                     EGVEKSYAIQAGREIRIIVNPEQMNDA"
     assembly_gap    736..745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1107..2477
                     /gene="miaB"
                     /locus_tag="PKJ95_01065"
     CDS             1107..2477
                     /gene="miaB"
                     /locus_tag="PKJ95_01065"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNR64862.1"
                     /translation="MRVYNIKENRKIFTYFIKTYGCQMNVNDSEYLSGQLEEMGYLMT
                     TNLHSADLIILNTCCVREKVEQKIFSMAGRVSQIKKEKPELIFGICGCLAQKEIENIQ
                     KKIPDIDLIFGPSQIASFQKIIELFWQNEKKKTITWCDNSEPFKLTDKPIKYFSSITA
                     YVQIMKGCNNFCSYCIVPYTRGPEESRPSDEILNEIKTLRKKNYKEVMLLGQNVNSYG
                     QELNNGDNFLTLLSKVNEIEGIERVRFITSHPKDFNLNLIKVIKNGSKICEHIHLPLQ
                     SGSNDILKKMNRKYDIKQYYNIIENIRKYIPGASITTDVMVGFPGESEEDFQETVNAF
                     QNIQFDSAFTFIYSNREKPLSSFFNEGIPLITKKKRLWTLIEIQKNISNNINQRMIGK
                     TVEVLVENRSKKGIENQYWGKTRTNKVVIFSDSKNRDLIAKLVYIKVKRADAYTLFGE
                     LADVKS"
     gene            2464..3420
                     /gene="miaA"
                     /locus_tag="PKJ95_01070"
     CDS             2464..3420
                     /gene="miaA"
                     /locus_tag="PKJ95_01070"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNR64863.1"
                     /translation="MSNLNKIAILTGPTAIGKTEISIKVARFISDIEIISADSMQIYK
                     NMDIGTDKPGKNILNRYKHHCIDLVEPWEAFSVAQYVKQAKKCISDILSRNKKPMIVG
                     GTGLYIKSLINPIFEGPGRNQKIRDTFYKVAKEKGNLFLYEELKKYDPEYSEKININD
                     TRRIVRALEVLYETGKPMSYFHKKTFNNGSKEDYQYLIICIYRNRDNLYQKINERVDN
                     IIENGLINETKLLMEKYDNINNLNAMQGLGYKQILLYLQGYIRKDEAIDIIKKKTRHF
                     AKRQMSWFRNQIKIDYWIDLDQYQNPEDGTLKIVDIMKREGY"
     gene            complement(4226..4570)
                     /locus_tag="PKJ95_01075"
     CDS             complement(4226..4570)
                     /locus_tag="PKJ95_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HNR64864.1"
                     /translation="MNIIEKYQFGKITVNGSSYDKDLIIFPDFIQDNWWRREGHFLRI
                     TDLETIINYRPDILIIGTGMYGLMKVDNNLIQVLKDYNVKKVIVEKTKKACIYFNNET
                     IQKKVAALHLTC"
     gene            complement(4633..5499)
                     /gene="amrB"
                     /locus_tag="PKJ95_01080"
     CDS             complement(4633..5499)
                     /gene="amrB"
                     /locus_tag="PKJ95_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNR64865.1"
                     /translation="MKYKRRAVVAGSFYAANQQSLEQQIKDCFLHEIGPGVLPNNEQK
                     KERKIIGIISPHAGFMYSGPVAAHNYLQLSFEKAPKTIIILGPNHRGIGGEISLISSG
                     QWETPLGTVEIDQSISKKILAHDEKRIIRDDSEAHLYEHSIEVQLPFLQFIYTTEEYK
                     FVPISITNQQLSMMNYLSQIIYEIIKDNYRDFLLIASSDFTHYEDQESTRYKDFEGIK
                     EILKMDSELFYKTIQNNRASICGPGPISVIMDTCKKLRIDKGQLLKYATSGDVSGMYE
                     QVVGYAAINFKK"
     gene            complement(5532..>5807)
                     /locus_tag="PKJ95_01085"
     CDS             complement(5532..>5807)
                     /locus_tag="PKJ95_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64866.1"
                     /translation="HFALATRYSKENRYQDALANYKIVLKNQPNYPELKINYYLTYIK
                     YFVNSIFLKIVIALIVLAIVLFFVILKFSNKKKKSGKHYRSSSSNRF"
BASE COUNT         2187 a          803 c          947 g         1860 t
ORIGIN      
        1 agaatagcag gggttgacct tattgtagat gatacaccag aagcagtaat tatctctggt
       61 tttgatccta ttagacgaga aattgccaga attgccctgg aaaaattgat tacagacggc
      121 aggattcagc cagccagaat tgaagaagtg attgaatcct ccaaaaagat tgttgaaaat
      181 aatattagag aagaaggcga aaaagctgta tttgagacta atgttaggaa tttaaacccg
      241 aaattaatta atatcctggg taaactgaaa tacagaacaa gctatggaca gaatgttctg
      301 caacattcta aagaagtagc ttattttgca ggaatgatgg ctgatgagct tggtttaaat
      361 tcaagtttaa gcagaagagc agggcttctc catgacattg gaaaatctgt taatatggaa
      421 gtggaaggac cacatgcttt aataggagca gatattgcca ggaaaaatgg agaatcgcca
      481 aaagtagtaa acgctattgc cgctcatcac gaagatgaaa aaccagaatc agccgaggcg
      541 attttgatac aggcagcaga cgctatctct gcttccagac ctggtgccag aagagagatg
      601 ctggaaactt atatcaagag gctgcaacag ttagaagaaa ttgccaattc ctttgaagga
      661 gtagaaaaat cttatgccat tcaagctggt cgtgaaataa gaataattgt aaatcctgaa
      721 cagatgaacg acgcannnnn nnnnnaatcc agtcgaaata gatgattatc tggcgacaaa
      781 actatccaga gatatctcta aaaaggtgga aaagaacatc gattaccctg gccaggttaa
      841 gataaatgtt atccgggaaa cacgctcggt agagtatgcg aagtaatttt taaattggct
      901 taaaggtttg tggtaaggaa tttagaagaa ggaggtattt tataaaaata tctccttctt
      961 ttttattccg aaatcatttt ataccatact ttataattaa aactttgtat atagaaacag
     1021 cataaaaatt agaatagatt aataattaga tattgtttta aatataagac agcccagata
     1081 cgacaaaact taatggagtg caataaatga gggtgtataa tataaaagaa aacagaaaaa
     1141 tatttaccta ttttattaaa acatatggtt gtcaaatgaa tgtaaacgac tcggaatatc
     1201 tgtcaggaca acttgaagag atgggttacc taatgacaac caatttacat tctgctgact
     1261 tgattatact aaacacctgt tgtgttagag aaaaggttga gcaaaaaatt ttcagtatgg
     1321 ctggtagagt cagccagatc aagaaagaaa aacctgagct aatttttgga atctgtggtt
     1381 gcctggctca aaaagaaatt gagaatattc aaaaaaagat acctgatatt gatttaatat
     1441 ttggtccttc tcaaattgct tcttttcaga aaataataga gctattctgg caaaatgaaa
     1501 agaagaaaac tattacttgg tgcgataata gtgaaccttt taagttaaca gacaaaccaa
     1561 ttaagtactt cagttcaata actgcatatg tccagattat gaaaggatgt aataatttct
     1621 gttcttattg tatcgtgccc tacactagag gtcctgagga gagcaggcct agtgacgaga
     1681 tattaaatga aattaaaaca ctgagaaaaa aaaattataa agaagtaatg ttgttaggac
     1741 agaatgttaa ctcttacggg caggaactaa ataatgggga taatttttta actctacttt
     1801 ccaaggttaa cgagatagag ggtattgaga gggtcagatt tattacctca cacccaaaag
     1861 actttaatct taatttgatt aaagtaatta aaaatggtag taaaatatgt gaacatatac
     1921 atctgcctct tcagtccggc tcaaatgata ttttaaaaaa aatgaataga aaatatgata
     1981 taaaacaata ctataatatt attgagaata ttagaaagta tataccaggt gccagtatta
     2041 ctactgatgt gatggttgga tttccgggag agagtgaaga agattttcag gaaacagtca
     2101 atgcctttca aaatatacag tttgattctg cctttacttt tatttattct aatcgggaaa
     2161 agcccttatc ctcctttttt aatgaaggaa tacctcttat aacaaagaaa aagcgtttat
     2221 ggaccttaat agaaatacaa aaaaatataa gtaataatat aaatcagcgt atgataggaa
     2281 aaacagtaga agtgctggta gaaaataggt ctaaaaaggg gatagaaaac caatattggg
     2341 ggaaaaccag aaccaacaaa gtagttatat tttctgattc gaaaaataga gatttgattg
     2401 ctaaattggt ctatataaaa gtgaaaaggg ctgatgctta caccttattc ggagagttgg
     2461 ctgatgtcaa atcttaataa gattgcaatt ttaactggcc caacagccat tggaaaaaca
     2521 gaaatatcta tcaaagtagc ccggtttata tctgatatag agatcatttc tgctgattca
     2581 atgcaaatat ataaaaatat ggatattggc acagataaac caggtaagaa tatattaaac
     2641 agatataaac atcactgcat tgatttagtg gaaccttggg aagcttttag tgtcgcccaa
     2701 tacgtcaaac aagccaaaaa gtgtatttct gatatattga gtagaaacaa aaaaccaatg
     2761 attgtaggtg gaacagggct ttatataaaa tcacttataa accctatatt tgaaggtccc
     2821 ggccgaaacc aaaaaattag agatacattt tataaagtgg ctaaagaaaa ggggaaccta
     2881 tttttatatg aggagttaaa aaaatacgac ccggaatatt ctgaaaagat aaacattaat
     2941 gacaccagaa gaatagtaag agctttagaa gttttatatg aaaccggtaa accaatgtct
     3001 tattttcaca aaaaaacatt taataatgga agcaaagaag attatcaata ccttataata
     3061 tgtatctaca ggaataggga caatctttat cagaagatta atgaacgggt tgataatatt
     3121 atagaaaatg gtttaataaa tgaaacaaaa ttattaatgg aaaaatatga caatataaat
     3181 aatctcaatg ctatgcaggg tttgggatac aaacagatat tattgtattt gcaaggatat
     3241 ataagaaaag atgaagctat tgatattatt aaaaagaaga cccgtcattt tgcaaaaaga
     3301 caaatgagct ggtttagaaa tcagataaaa atagattact ggattgactt agatcagtac
     3361 caaaatccag aagatggtac cttaaaaatt gttgatataa tgaagaggga aggatattga
     3421 ttaatataat gaaaatctta tatcattata aatgatgcag tttgcataaa tattaaatat
     3481 atttatatct tccgataata tatcttatgt aaagtaaaaa gagatatcaa taaaaccgat
     3541 cagcattgtt aaatctggtt tattctttag atctatactt tgtatactct cgggtggtaa
     3601 ccaattttga taatttattt ttagagagcc ctcatctatt ttacaatcta aaatcttttt
     3661 atgatgtttt taattaatta tttgacatta gaatattaat gaaaaaatta aatgaaacat
     3721 tttctaatac aaaatatttt aagaaaatga aatatctttg tttgcccttt taataaattc
     3781 atatttctca atttctatga aaatgtataa aaacgaaatt gattaaatat cattcattat
     3841 caaaacagtt cacatcttta aaaaacctta aaaacgtttc taaatgaata tttgattact
     3901 aagatgatat ttatatataa attagtattt agattctaaa atttgtgtta tttagtgcat
     3961 ataagatgga attggtgaag aatagaaatt tatggagagt ttttaactta ttaactacct
     4021 attatattaa gattttacta aaaatatcag tcaaaaaata taaccaaaat caaatatgat
     4081 tattgaaaca ttattaataa acttataggt ataatattct ttatgatggt atttatattt
     4141 tcaataaata ttcaaaaaag acattgtttg caacatttac atacaaaaga ataatataat
     4201 aaatatataa aaggtagaaa aaaagctaac aagttaaatg tagagcagca acttttttct
     4261 gtatagtttc attattaaaa tagatacagg cttttttagt tttttctact attacttttt
     4321 tgacattata atccttcaaa acctgtatca aattattatc aactttcatt aaaccataca
     4381 ttcccgtgcc aatgatcaga atatccggtc tgtaattaat gatggtttcc aaatctgtta
     4441 ttcttaaaaa gtggccttct ctcctccacc aattatcctg gataaaatca ggaaagataa
     4501 ttaagtcttt atcgtaactt gaaccattga cagtaatttt accaaactga tatttttcga
     4561 taatattcat atatcttaaa agtattatat ttattttaat tatgctagat aagcattatt
     4621 aaatattact aattattttt taaaatttat tgcagcatag cccacaacct gttcatacat
     4681 accagaaacg tcacctgaag tagcatattt taataattgt cccttatcta ttcttaattt
     4741 tttgcaagta tccattatta cagaaattgg tccggggcca cagatagagg ctctattatt
     4801 ctgaattgtt ttataaaaaa gttcgctatc cattttcaat atttccttaa ttccttcgaa
     4861 atctttatac ctggtacttt cctgatcttc ataatgggta aaatcagaac tggcaatcaa
     4921 aaggaaatcc ctataattat ctttaattat ttcatagatt atctgtgata aataattcat
     4981 catagaaagt tgctgatttg taattgaaat aggaacgaac ttatactcct cagtggtata
     5041 gataaattgt aaaaatggca attgtacttc aatagaatgt tcataaaggt gagcctctga
     5101 atcatctcgt ataattcttt tttcatcatg agcaagaatt tttttgctta tcgattgatc
     5161 aatttcaact gttcctaaag gagtttccca ttgaccggaa gaaataagtg aaatttcccc
     5221 tcctattcct ctatgattag gacccagtat aataatagtt tttggtgctt tttcaaagga
     5281 aagctgcaaa taattatgtg cagcaaccgg tccagaatac ataaatcctg catgtggaga
     5341 aattatccct attatttttc tttctttttt ttgttcattg ttgggcagta ctcctggtcc
     5401 aatctcatgt aaaaaacaat ctttaatttg ttgctctaag gattgctgat ttgcagcata
     5461 aaaagatcca gctacaacag ctcttctttt atatttcata aaagaatccc ccttaaaata
     5521 ttgataaaaa tctaaaatct attactgcta ctgcttctgt agtgtttacc gctctttttc
     5581 tttttatttg aaaatttgag aataacgaag aataacacaa tagccagtac aataagggca
     5641 ataacaattt ttaaaaatat tgaattaaca aaatatttaa tgtaggttaa ataatagttt
     5701 atttttaatt ccggataatt aggctgattt ttaaggacaa ttttataatt ggctaacgca
     5761 tcctgataac gattctcttt tgagtaccgt gttgctaaag caaagtg
//
LOCUS       DAOQWT010000025         5797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_14150_length_5797_cov_3.090909, whole genome shotgun sequence.
ACCESSION   DAOQWT010000025 DAOQWT010000000
VERSION     DAOQWT010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14150_length_5797_cov_3.090909"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="PKJ95_01090"
     CDS             complement(<1..1726)
                     /locus_tag="PKJ95_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNR64867.1"
                     /translation="MSKSISLIFILLIIIIAGFAYFNYNQLNRIQSDLLKKEALLEES
                     ERLIEEQGTSLVQMEEKIKEALEKDKILSDEMIMAEKEDKLQKQLEGYQEELLALQLK
                     MAEVLQKNISDTESISLLREEKSKLESSLEERETQWLEKEKDNKEIISSLQEGIRQYE
                     TEMELVNKKVLNIEQYLETEQLKRQELEQDIKKYEDTIDYLKEQLALKEDEESYVQQI
                     SKLEKDKKELEEEVAEKDSSLIQFYEEYQNLQNQLADYERKINQLTVETAQVLEQKGI
                     LAEIKDNLTKLEQEKSKMEKILQEKETQWLEKEQVDKEIIAQLQEEIKKYELNIKEIG
                     KEILVLQEDLIKEKALQEPVQKEIDISSLEKLLADKEIEWAKQNEQNRRLISYLKGQL
                     EDYKKEIDIVKLDSSLFKEEIATQIDIYQQNLTQIGEKESQISLLTSQIELYMQKIDD
                     YEKSVAEFKMRLQEQEDINYQEQQSLIETIHSLVKEREEYQNSINQYYAQVNKFQLEI
                     AQLKNKIGVLESEKEPKYYEVKSGDCLWNIARNKYNEGIAWIKIFRANEELIQNPEYL
                     YPGDTLIIF"
     gene            2002..4446
                     /locus_tag="PKJ95_01095"
     CDS             2002..4446
                     /locus_tag="PKJ95_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR64868.1"
                     /translation="MDIQKNTFPEVPVEQLRWSCDLKKAHFSSTEDLKACDKILGQER
                     ALKALELGLEMEYLGYNLFITGKSGTGRSTSIKQLLQNRKRENVIFDDKCYVHNFKDP
                     DMPRAISLPAGQGNLFKKDMENLVNTLRKHIPLLLESEKYQESKDSLTENFKKKQQSL
                     IKEFEDKVKADNFTIVQIQIGPYTRPDIFPIIDKKPTNIENLEALVGEGKFSKEELDT
                     IKKKHHEFTQEIAVVSKKINALQKELSEKLTDLEIKIITPLVEEEIAEIKKRYDNDAI
                     NSHLKEVKKNVLENISRFQEKEEQAPVIPGLRLPQIKDSFIEFSVNVLVDNSDTKTSP
                     VIIETRPTYKNLFGIIERRLDRTGYWVTDFTKIKAGSLLKANGGFLVLNALDVLLEPG
                     VWPSLKRTLLNQMIEPETYDPFPMFSTSALKPEPVECNVKVAMIGDPFLYQLLYFRDP
                     DFEKIFKIKADFDTVTGNTEQNIYQYSCFIKQLCERENLLPFDQSGIEGVIEYAIRLA
                     GRKNKLSTHFNNLVDLLREADYWAKKDKQNLIQRKHITTALKEKIERLNLIEDKIQEL
                     IEQGTLMIDTEGAIVGQVNGLSVYDLGEYSFGKPTRITAKTSMGRAGIINIEREADLS
                     GKTHNKGILILSGYLRSKYAQDKPLTISASICFEQSYSGVDGDSASSTEAYALISSIA
                     DIPIRQDIAVTGSINQKGEIQPIGGVNQKVEGFFEVCRAKGLTGTQGVIIPESNIPDL
                     MLREDVVETIKSGQFHLYPVKTIDQGIEILTGRKAGIRKLDGKFEKDSVNELVDQKLL
                     DFALKLKEFGAEKKKK"
     gene            4538..5647
                     /locus_tag="PKJ95_01100"
     CDS             4538..5647
                     /locus_tag="PKJ95_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR64869.1"
                     /translation="MKRKVRTGLTITCFLLFFLVLFYSPISASDKINLEALLQSIIEE
                     KTVTTDVYAVINLGNFFFSEKLYSPARDEYVKALQIDPCNKIALINLSYTFFQMGDFE
                     KALTQLVPLREDDVAYSYYIRGMIFKEQRKLEEAITQYEKVVELIPNHSQLYAELGQL
                     YLDNHQLVKANERFIEMGYCKYRPPIMDKLVDYQPNAYCYLHLGNYYRNNGELDLARE
                     AYQKVTQFEDDLRSIALAYYYQGEINLKDHNYDRAIIEKELSQKVYPLGGHQFTFNSF
                     AEALIEIGDQYYHNGNLPEALTHYQLAANLANKPDILAEAHYKKGLTYYRSQDYENAL
                     KEAETALSINPDYLSDRQRLIDLLIANSWSNITQK"
BASE COUNT         1879 a         1036 c          960 g         1922 t
ORIGIN      
        1 taaatataat gagggtatcg cctggataaa gatattcagg attttgaatc aattcctcat
       61 tagccctgaa tatctttatc caggcgatac cctcattata tttatttctg gcgatgttcc
      121 agagacaatc tccactttta acttcgtaat attttggttc tttttcactt tctaagaccc
      181 ctattttatt ctttaactga gcaatctcca attgaaactt attaacctgg gcatagtact
      241 ggttgatact attctggtat tcttctcgtt ctttcaccag agaatgaata gtttctatta
      301 aactctgctg ttcctgataa ttaatatctt cctgttcttg taatctcatt ttaaattctg
      361 ccacagactt ttcataatca tctatttttt gcatatatag ctcaatctgc gaggttaata
      421 atgaaatctg ggactctttc tctccaatct gagttaggtt ttgctgataa atatctatct
      481 gtgtggcaat ttcttcttta aacaggctgc tgtccagttt gactatatca atctcctttt
      541 tataatcttc cagttgccct tttaggtaag agattaacct cctattctgc tcattttgct
      601 ttgcccattc tatttcttta tcagccaaca gcttttccaa actactaata tctatttctt
      661 tttgtacagg ttcctgcaat gccttttctt ttattagatc ttcctgtaat acaagtatct
      721 ctttgccaat ctcttttatg tttaactcgt attttttaat ttcttcctga agttgagcaa
      781 tgatttcttt gtccacttgc tctttctcca gccattgagt ttctttctct tgcaaaatct
      841 tctccatctt gcttttctcc tgttccagct tagtcaggtt gtcctttatt tctgctaata
      901 ttcctttctg ctccaatact tgagctgtct ccactgttaa ctgattaatt tttcgttcat
      961 agtcagccag ttggttttgt aaattttgat actcctcata aaactgaatc aggctggaat
     1021 ctttctcagc aacctcttct tctaattctt ttttatcttt ctctaacttg cttatctgtt
     1081 gaacataaga ctcttcatcc tcttttagag caagttgttc ttttaaataa tcaatagtat
     1141 cttcatattt ctttatatct tgttctaatt cctgcctttt tagttgttct gtttctaagt
     1201 attgttctat atttaaaact tttttattta ctaattccat ttcggtctca tattgccgga
     1261 tcccctcttg caaggatgag ataatctctt tattatcttt ttctttttcc agccattgag
     1321 tttctctttc ttcaagtgag gattctaatt tggatttttc ttctcttaaa agggaaatgc
     1381 tttcagtatc agatatattt ttctgtaaaa cttctgccat ttttaactgc aatgccagca
     1441 attcttcttg atatccttcc agctgttttt gaagtttatc ttccttctct gccattatca
     1501 tctcatccga aagaatttta tctttttcca aagcttcctt tattttttct tccatctgaa
     1561 ccagggaagt cccctgttct tcaatcaatc tttccgattc ttctaataat gcctcctttt
     1621 tgagtaaatc actttgaatc cgattcagtt gattataatt aaaataggca aaaccagcta
     1681 ttataattat taataaaata aagataagcg aaatactttt tgacattgta ttcctcccct
     1741 gtttactcta atattcttta ttatatctta aaaagagaaa cggttcacca ttatactaaa
     1801 aagtcatttt aatatttaat ctttattttt ttaagcagat ttagctacaa atataatcct
     1861 tgcttctaat cttgctattt gttccttaac aaatataaaa tatactgcta taataaaaag
     1921 aaatattcct gtcaataaca atgtaataaa taaaaaaaat taaaaaactc ttttcctaaa
     1981 agagcattaa aaggagggat tatggacatt caaaaaaata cctttcctga agtccccgtt
     2041 gaacagttgc gatggagttg tgatttgaaa aaggctcatt tttcttccac tgaggacctg
     2101 aaagcctgtg ataaaattct gggacaagaa agagctttaa aagcattgga attaggcctg
     2161 gagatggagt atcttggtta taatctcttt attaccggga aatcaggtac cggcagaagt
     2221 acttccatta aacaactatt acagaacaga aaaagagaaa atgtcatctt tgatgacaaa
     2281 tgttatgttc ataacttcaa agaccctgat atgcccagag caatcagtct acccgccggg
     2341 cagggtaatc ttttcaaaaa agatatggag aatctggtaa acaccttaag aaaacatatt
     2401 cctttgcttt tagaaagtga aaaataccag gaaagcaaag attctctcac tgaaaatttc
     2461 aaaaagaaac aacaatcttt aattaaagaa tttgaagata aagtaaaagc agacaatttt
     2521 actattgttc agatacagat aggcccttat accaggcctg atatctttcc catcattgat
     2581 aaaaaaccaa caaatataga aaatttagaa gcattggtcg gggaaggaaa attttccaaa
     2641 gaagaattag atacaatcaa aaagaagcat catgaattta cccaagaaat tgctgtggtt
     2701 tccaagaaaa ttaatgccct tcaaaaagaa ctcagtgaaa aattaacaga tttagaaata
     2761 aaaataatca cccctctggt agaagaagaa attgctgaaa taaagaaaag atatgataat
     2821 gatgccatta actctcacct gaaagaagtt aaaaaaaatg ttctggagaa tatcagccgt
     2881 tttcaggaaa aagaagaaca ggcaccagtt attccaggat taagattgcc tcaaataaaa
     2941 gattctttca ttgaattctc agtaaacgta ctggtcgata attctgatac caagacctcc
     3001 cctgttatta ttgaaacccg ccctacctat aaaaatcttt tcggcataat tgaaagaagg
     3061 ttggaccgca ccggttactg ggtaacagat tttactaaaa taaaggcagg ttctctttta
     3121 aaggccaacg ggggtttttt ggtattgaat gccttagatg tattacttga accaggtgtc
     3181 tggccctctt taaaaagaac tcttttaaat cagatgattg aaccggaaac ctacgacccc
     3241 tttcctatgt tcagtacctc tgccttaaaa ccagaaccag tggaatgcaa tgtaaaggta
     3301 gccatgatcg gagatccttt tctctatcag cttttatatt tcagggatcc ggattttgaa
     3361 aaaattttca aaataaaagc ggattttgat accgttactg gaaatactga acaaaatata
     3421 tatcaatatt cctgttttat caagcaacta tgtgagagag agaatttatt accctttgac
     3481 caaagcggaa ttgaaggggt aattgaatat gctattagat tggcaggaag aaagaataaa
     3541 ttatccaccc atttcaataa ccttgttgac ctcttgagag aagcggatta ctgggctaaa
     3601 aaagataagc aaaatcttat tcaaagaaaa catattacta cagcacttaa ggaaaaaatt
     3661 gaaagactga atctcattga agataaaatt caagaactga ttgaacaggg aaccttaatg
     3721 atcgatactg aaggcgcaat tgtaggccag gtaaatgggc tttccgtata cgatctgggc
     3781 gagtattcct ttggcaaacc aaccagaata actgccaaga cttcaatggg cagagcagga
     3841 attattaata tcgaaaggga ggctgacctg agtggtaaaa cgcataataa aggcattctt
     3901 atattgagtg gctatctgcg ctcaaaatat gcccaggata agcccctaac cattagtgcc
     3961 agtatttgtt ttgaacaatc ttattctgga gtagatggag atagcgcttc ctctacggaa
     4021 gcttatgctc taatttccag tattgcagat attcccatca ggcaggatat tgcagtaacc
     4081 ggttcaataa accaaaaagg agaaattcag cccataggtg gagttaatca gaaagttgaa
     4141 ggattttttg aggtctgccg ggcaaaaggt ttaacgggaa ctcaaggggt aataataccg
     4201 gaatcaaata ttccagacct tatgttgaga gaagatgtgg tggaaacaat taaaagcggt
     4261 cagttccatc tttatcctgt taagacaatt gatcagggaa tagagatatt aactggaaga
     4321 aaagctggca ttagaaaact ggatggaaag tttgaaaaag attctgtcaa tgaactggtg
     4381 gaccaaaaat tattagattt tgctctaaaa ttaaaagaat ttggtgcaga aaagaaaaaa
     4441 aaataatata atggaatcag atatacgtta tatatggaag taaagcgagg caagattact
     4501 tatagttata tttaataata attaaggaga tattataatg aaaagaaaag taaggactgg
     4561 gttaactatt acctgttttc ttcttttttt cctggtttta ttctactccc ccatctctgc
     4621 cagcgataaa ataaatttgg aggcactttt acagtctatc attgaagaaa aaacggttac
     4681 cacagatgtt tacgcagtta ttaatttagg aaatttcttt ttttcagaaa agttgtattc
     4741 tccagcccga gatgaatatg ttaaggcatt gcaaatagat ccctgtaata aaattgcctt
     4801 aattaatctc agttatacct tctttcaaat gggtgatttc gaaaaagccc tcacccaact
     4861 ggttccctta agagaggatg atgttgccta ttcttattac atcaggggaa tgatttttaa
     4921 agaacagcgc aaattagagg aagctatcac ccaatacgaa aaagttgtag agctaatccc
     4981 caatcattct caactatatg ccgaattagg tcaactctat ctcgataacc atcagctggt
     5041 aaaagcaaat gagaggttca ttgaaatggg ctattgtaag tatcgtccgc caataatgga
     5101 caaacttgtt gactatcaac ctaatgccta ttgttatctg catttgggaa actattaccg
     5161 caataatgga gagctggatc tggcacggga agcatatcag aaagtaactc agtttgagga
     5221 cgacctgcgt tctattgccc tggcttatta ttaccaggga gagattaatc ttaaagacca
     5281 taattatgat cgggctataa ttgaaaaaga gttgtctcaa aaagtttatc ccttaggcgg
     5341 gcaccaattt acttttaaca gtttcgcaga agctcttatt gaaattggtg atcagtatta
     5401 tcataatgga aatttacctg aagcacttac ccattaccaa ttggctgcta acctggccaa
     5461 taaaccggat attttggctg aagcccacta caaaaaaggt ttgacttatt atcgcagtca
     5521 ggattatgaa aatgctttaa aagaagccga aactgcactg tcaattaatc ctgattatct
     5581 gtctgacaga caaagattaa tcgatttatt gattgccaac agctggtcta atattactca
     5641 aaaataataa attgagccag tatatgggga agtaatataa ttaccttata aatgtaatgt
     5701 gggtaaaaat atgtgataga attttcaata tctccaaatt ttagagagga gttctaaaaa
     5761 tgagcttaac acggttttta aaaggatttg tcattat
//
LOCUS       DAOQWT010000026         5704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_14409_length_5704_cov_3.415826, whole genome shotgun sequence.
ACCESSION   DAOQWT010000026 DAOQWT010000000
VERSION     DAOQWT010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14409_length_5704_cov_3.415826"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..751)
                     /locus_tag="PKJ95_01105"
     CDS             complement(62..751)
                     /locus_tag="PKJ95_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR64870.1"
                     /translation="MENILIIEDQQSIAELIKDYLEINNFGVDIKNNGLEGLEEALSK
                     DYDLILLDLMLPDLDGFEICKKIRKEKNIPILIISARGEDIDKIRGLGLGADDYISKP
                     FSPNELVARVKAHLSRFNRLTGKTEKTKNEISIKNLTINQIARRVYLDNKEITFTTKE
                     FEILNFLASHPNIVFSKERLYERIWGDDSYGDITTIAVHIKKIRDKIEKDPQKPRFID
                     TLWGSGYRFNA"
     gene            complement(783..2246)
                     /locus_tag="PKJ95_01110"
     CDS             complement(783..2246)
                     /locus_tag="PKJ95_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR64871.1"
                     /translation="MMTIQKRLWISNLILILITIVLLIGVSLIITEKSREFIGFPSSD
                     DQRQPPVLNRALLRADRIIMKTIESEPDQFLNTDYLNQIDNFLNSFSTALIVKKDNHI
                     VYFSPFMAEIDTNHQSFLEQQINISQGESDIVRISDTLFMKTIPISFTDNTNGSLFLI
                     NDATVASQELQTFKNSVIYILISFLIIMIIINTLITFFLSKQIVNPIIRLKNAAQQIQ
                     KGDYNFQLRAPSKDEIGALFESFEEARKQLKKSEETQKKYEQSRNELITNISHDLKTP
                     ITTIKGYLEGIRDGIPQSQEKQNKYLETIYQNAVHMESLIEDLFLLSKFDLDQSFYQF
                     ENINIKEYLADCYEELQFDFQEKGIKLEYRVDYDENKLVKADRQQLKRVILNIINNAI
                     NYKNAGNPKIEMILTEKEEEAQIEIRDNGIGVPDDILDKIFERFYKEDKSRSNRSRGT
                     GLGLYIARKIILDHGGSIWAKSQVGSGTSIFFTLPMT"
     gene            2534..4264
                     /gene="glgP"
                     /locus_tag="PKJ95_01115"
     CDS             2534..4264
                     /gene="glgP"
                     /locus_tag="PKJ95_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNR64872.1"
                     /translation="MEAKHSFLYDHEECRVRENKIAYFSMEICIDSKIPTYSGGLGIL
                     AGDTIRSAADLGVPLIAMTLLYKKGYLRQNIDEQGLQHEFPDEWNPQDELAKLPVQIQ
                     VEIEKRKVTVQCWLYVVKGIDGSKVPVYFLDTDLPENSDFDRSLSYYLYGGDDRYRLA
                     QEIVLGIGGVRMLKELGFQDILRYHMNEGHASLLALELLNNFYDPQKNDQWDIEQVKK
                     RCVFTTHTPVPAGMDQFSYQLAGQVLGDFIPYNLLKSLAGNEKLNMTQLALNLSHYVN
                     GVAKEHGAVSREMFPGYHIYYITNGVHSSTWTGQYFAELYDKYIPGWKSDPFSLRYAL
                     NIPDREVWNAHQKGKEELIDHINKLTSLEFDKEVLTIGFARRATAYKRADLILYDLNR
                     LIDISRKKGQIQLVFAGKAHPKDQPGKELIQKIISASRHSPLKENIKTVYLENYNMDL
                     GKILTCGVDLWLNTPRKPQEASGTSGMKAAHNGIPSFSILDGWWVEGCIEGITGWSIG
                     SAANVESNEEEEAESLYKKLEIDILPAYYQKRKQWINMMRHCIAINASFFNTHRMVLQ
                     YVSNAYLYSK"
     gene            4295..4936
                     /locus_tag="PKJ95_01120"
     CDS             4295..4936
                     /locus_tag="PKJ95_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64873.1"
                     /translation="MSGNDWINNFVERLTNIEGIFEVYVSLFLKYNHQFVFGLKGSKE
                     WIEEQGKRKAAISAIGGRTDWSKDKNLIQILKESCVQDIGIDVTIEDSPYTYFDYQHR
                     LKKIPIDLVRGEIRPQMVTFIQKSRYTTTPGMLIFSFAGSTRQKPQALQYSALFLAGE
                     SVLMQMFKSEKTVKELKQAGATFEERIQIPDNLHLYPTGSVNSLLRFLSYEVF"
     gene            5024..>5704
                     /gene="purD"
                     /locus_tag="PKJ95_01125"
     CDS             5024..>5704
                     /gene="purD"
                     /locus_tag="PKJ95_01125"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNR64874.1"
                     /translation="MNILVIGSGGREHTIVWKMAQSSLVDKIFCAPGNAGIAELAECL
                     EVETDNLSGLLEIARHNSIDCTIVGPEVPLSLGIVDLFEDNGYPIFGPRRKAAEIESS
                     KVFAKQIMNKYHIPTARSQDFEDYYEALSYLEKQSFPLVIKADGLAAGKGVIITKDRF
                     EAELALGKIMKERKFGEAGRRVLIEEFLTGEEVSMLVFSDGRNILPMITSQDHKKIGD
                     NDEGLNTGG"
BASE COUNT         1820 a          918 c         1096 g         1870 t
ORIGIN      
        1 aaacattttt taaaaattaa aacattaaaa tttttttata acacatcttt tctcttactt
       61 atcaggcatt aaaccggtat ccggatcccc atagtgtatc aataaagcgg ggcttttggg
      121 gatctttttc aatcttgtct cttatttttt taatgtgtac tgcaatagtg gttatatctc
      181 cataagagtc atccccccag atacgttcat agagccgctc tttgctgaag actatattgg
      241 gatgagaagc caaaaaattt aatatctcaa attctttagt ggtaaaggtt atctctttat
      301 tatccagata aacacgtctt gctatctgat ttatggtcaa gtttttaatg ctaatctcat
      361 tttttgtctt ttcggttttc ccggttaaac ggttaaaacg gctcaagtgt gccttaactc
      421 tggctaccaa ttcattgggg ctgaaaggtt tgctgatata atcatctgct cccaaaccca
      481 ggccacgaat tttgtcaata tcttctcccc gtgctgaaat aatgagaata ggaatattct
      541 tttcttttct gattttttta cagatttcaa aaccatccag atccggtaac attagatcga
      601 gcagtattaa atcataatct ttactcaaag cttcttctaa gccctccaga ccattgtttt
      661 ttatatcaac cccgaagtta ttaatttcca gataatcctt tatcagttca gcaatgctct
      721 gttggtcttc aataattaga atattttcca ttttcaatta ccctatttat accattatat
      781 ttttaggtca taggcagggt gaaaaaaata ctggttcccg aaccaacctg gctttttgcc
      841 cagatagaac caccatggtc aagaataatt tttcgagcga tatagagtcc caggccagta
      901 ccccgggaac ggttggaccg agatttatct tctttataga atcgttcaaa aattttatcc
      961 aaaatatcat ccggaacacc aataccattg tcccttattt ctatctgtgc ctcttcttct
     1021 ttttcagtta gtatcatttc gattttagga ttaccggcat ttttataatt gatagcatta
     1081 ttaataatat ttaaaattac tcttttcaac tgctgtctat ctgccttaac cagtttattt
     1141 tcatcataat ctaccctgta ttccagtttg atgccttttt cctggaaatc aaattgcagt
     1201 tcttcataac aatcagccag gtattcttta atattaatat tctcaaattg ataaaaagac
     1261 tgatctaaat cgaattttga aagtaaaaat aaatcttcaa ttagtgattc catatgaaca
     1321 gcattctgat agattgtctc taaatatttg ttctgcttct cttgtgattg aggtatacca
     1381 tctctaattc cctctaagta acctttaata gtggttatgg gagtttttaa atcatgcgag
     1441 atattggtaa ttagctcatt acggctttgt tcgtatttct tctgtgtttc ttctgatttt
     1501 ttcagctgtt tgcgggcttc ttcaaaactt tcaaacaatg caccaatctc gtctttgctg
     1561 ggtgcccgca attgaaaatt ataatcgccc ttttgaatct gttgtgctgc atttttaaga
     1621 cgaatgatgg gattgacaat ctgtttagag aggaagaaag ttatcagagt attaataata
     1681 atcattataa ttaaaaatga tatgagaata taaatgacag aatttttaaa agtctgtaat
     1741 tcttgagagg caactgtggc atcatttatt aagaatagac tgccatttgt attatcagta
     1801 aagctgatgg gtatagtttt cataaacaga gtatctgaaa ttctaacaat atcactttcc
     1861 ccctgtgaaa tattaatttg ttgttccaaa aaagactgat gattggtatc aatttcagcc
     1921 ataaatggag aaaaatagac tatatgattg tcttttttta caatcagggc agtgctaaaa
     1981 gaatttaaaa aattatctat ctgattgaga tagtcagtat ttaaaaattg atccggttct
     2041 gattcaatgg ttttcattat tattcgatct gcccgaagca gagctctgtt aaggacggga
     2101 ggctgtctct ggtcatcgga ggaggggaat ccaatgaatt cccgggattt ttctgttatg
     2161 attaaactga caccaattaa taatacaatg gtaatcagta tcagtattaa attggatatc
     2221 cataaacgtt tttgaatggt catcattttt tatggtctgt ttaccctttg ctttatagga
     2281 ctgattaata atatttactc tgtattcttc tattattata tgttattatt ttaaaatact
     2341 cataaaatat ttattaaaac ttcataaaat atggtattaa tagctaattg gttgtcaatt
     2401 attagaaatt ggataaatat gataaaattt aataagttta tcatgagttt aacgagccaa
     2461 tatcccttca gctaaaggat gaataatttt caataattta ttttataatt aagcgaaata
     2521 aaacggaggc aagatggaag caaaacatag ctttctttat gatcatgaag aatgtagagt
     2581 tagagagaat aagattgctt atttttctat ggaaatttgt attgactcca aaataccaac
     2641 ctatagtgga gggttgggaa ttttagctgg tgataccatt cgctcagccg cagatttggg
     2701 agttcctttg attgctatga ctctactata taaaaaaggc tatctcaggc agaatattga
     2761 tgaacaggga ctccagcatg aatttcctga tgaatggaat cctcaggatg agctggctaa
     2821 attaccagtt caaattcaag tagagataga gaagagaaag gttactgtcc aatgctggtt
     2881 atatgtggta aaaggaattg atggcagtaa agtaccagtt tattttttgg atacggattt
     2941 acctgagaat agcgattttg accgtagtct ttcttattat ttatatggcg gagatgaccg
     3001 ttaccgttta gcccaggaga tagtattggg aattggcggt gtaagaatgt taaaagaact
     3061 gggatttcag gatattttga gataccatat gaatgaagga catgccagtc tgttagccct
     3121 ggaattgctg aacaattttt acgatcccca aaaaaacgac caatgggata tcgaacaggt
     3181 gaaaaaaagg tgtgttttta ccacgcatac gccggttcct gctggaatgg atcagttttc
     3241 ctatcaatta gccgggcaag ttctgggaga ttttattcct tataatttat taaagagtct
     3301 ggctggtaat gaaaaactca atatgacaca gttagctttg aatttaagcc attacgtcaa
     3361 tggagttgct aaagaacacg gggcagtatc tcgagaaatg ttcccaggat accatattta
     3421 ttatattacc aatggggttc attctagtac ctggactgga caatattttg ctgaattata
     3481 tgataaatat attcccggct ggaaatctga cccattcagt ctgcgttatg ccctcaatat
     3541 tccagataga gaggtatgga atgcccatca gaaaggcaag gaagagttga ttgaccatat
     3601 taataaactt accagtttag agtttgataa agaggtttta acaattggtt ttgccagaag
     3661 ggctactgct tataagcgcg ctgatctgat tctttatgat ttaaatcgtt taatagatat
     3721 ttcccggaaa aaaggccaaa tccaacttgt ttttgccggc aaggcgcacc ctaaagatca
     3781 gccaggcaag gagctaattc aaaaaattat cagcgcttcc cgacattccc ccttaaagga
     3841 gaatataaaa acagtctatt tagaaaatta taatatggat ttaggaaaaa tattaacctg
     3901 tggagtagat ttatggttaa atactcccag aaaaccccag gaagcatctg gaacttcagg
     3961 aatgaaagcc gcccataatg gaattcccag ttttagtatt ctggatgggt ggtgggtgga
     4021 aggatgcatt gaaggtatta ccgggtggtc tataggttct gcggcaaatg tagagagtaa
     4081 cgaagaggaa gaagcagaat ctctctataa aaaattagaa atagatatat tgcctgctta
     4141 ttatcaaaaa cgaaagcaat ggataaatat gatgaggcac tgtattgcca ttaatgcctc
     4201 tttctttaat actcaccgta tggtattgca atatgtctct aatgcctatc tctacagtaa
     4261 ataaaaaaag tttagagagg aaggttaaaa ataagtgagt ggtaatgact ggataaacaa
     4321 ttttgtagag cggttaacaa atatagaagg aatttttgaa gtatatgttt ctctcttttt
     4381 aaagtataac caccaatttg tttttggttt aaagggcagc aaggaatgga ttgaggaaca
     4441 ggggaaaaga aaggcggcaa tatctgctat tggtggtcga actgattgga gtaaagataa
     4501 gaacttaata caaatcttaa aagagagctg tgttcaggat attggaatag atgttaccat
     4561 tgaagatagc ccttacactt attttgatta tcaacatcga ttaaaaaaaa tacctattga
     4621 tttagttagg ggcgaaataa gaccacagat ggttactttt attcagaagt caaggtatac
     4681 aactactcct ggtatgctta tctttagttt tgcaggaagc accagacaga aacctcaggc
     4741 tttacaatac agcgccctct ttttagccgg ggaatcggta ttaatgcaaa tgtttaaaag
     4801 cgaaaagaca gtaaaagaat taaaacaagc cggagcaact tttgaagaac ggatacagat
     4861 tcctgataat ctccatcttt acccaacagg ttctgtaaac agtttattac gttttttaag
     4921 ttatgaagtc ttctaaaatt taaagagggg tcattacaga agatatttaa caaaatcggt
     4981 agataagttt gttatattaa taatagatga tgaggatata aatttgaata ttctggtaat
     5041 tggtagcgga ggtagggaac atactattgt ctggaagatg gcgcaaagtt ccttagttga
     5101 taagattttc tgtgcgccgg gcaatgctgg catcgccgaa ctggcagaat gtctggaggt
     5161 agaaacagat aatttatctg gtttattaga gattgcccgc cataatagta tagattgcac
     5221 cattgtagga cctgaagtgc ccctctcttt gggaatagta gacctttttg aagataatgg
     5281 ctatcccatt tttggacctc gtcgaaaagc tgctgagatt gaatcaagta aagtctttgc
     5341 caaacaaata atgaataagt accatatacc aacggcccgc tcacaggatt ttgaggacta
     5401 ttatgaggct ctttcttatc ttgaaaaaca gtctttccct ttagtaatta aggcagatgg
     5461 attagctgcc ggaaaaggag ttattattac aaaagataga tttgaggctg aattggctct
     5521 tggaaagatt atgaaagaga gaaaatttgg tgaagcaggc aggcgggtat taattgaaga
     5581 atttcttacc ggcgaggaag tttctatgct ggtcttcagt gatggcagga atattctacc
     5641 tatgataaca tctcaggacc ataaaaaaat tggtgataat gatgaagggc taaatacggg
     5701 aggt
//
LOCUS       DAOQWT010000027         5636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_14630_length_5636_cov_3.341695, whole genome shotgun sequence.
ACCESSION   DAOQWT010000027 DAOQWT010000000
VERSION     DAOQWT010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14630_length_5636_cov_3.341695"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PKJ95_01130"
     CDS             <1..364
                     /locus_tag="PKJ95_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNR64875.1"
                     /translation="RIGDIGVYLNAQQQSLLSPWFKYFLTYDPGPALSMVKCPVLALY
                     GGKDLQVPSVENLKATESALIKGGNKDFTVKEFSGLNHLFQTAQTGSPDEYGRIEETI
                     SPRVLEFISDWIWRHVEN"
     gene            454..939
                     /locus_tag="PKJ95_01135"
     CDS             454..939
                     /locus_tag="PKJ95_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64876.1"
                     /translation="MKVLRLQYEKNEILKKNKAIEVCFKCSTCSKFCPITQYVKKYNQ
                     ENSFVTHLFEAEKSEALNDVWMCCACEKCVITCPQDTNPTDAFTNLKEKSYREGYAPE
                     IVYRLMEQLLQEGTVYNIKFSNAARKRAGLTEIEKNERAIKELNILADKTGLVRGKGN
                     N"
     gene            939..1820
                     /locus_tag="PKJ95_01140"
     CDS             939..1820
                     /locus_tag="PKJ95_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNR64877.1"
                     /translation="MINYLFFKGCTIPANLPNIEKLTLEILPEIGINLSVSDEFTCCP
                     DPIRLQGANQYFWMVSAARNITIAEEKNMNIMTLCNGCENTLGIVNYKLKNNPELKSK
                     VNEVLKDIGRELKSIIEVKHFLQVLKEDIGLDNLKQMIKNPLSGFKIAGHPGCHLLMP
                     NEILKFDNPVDPEFYDQFIAALGATPIDYLSKIDCCGVSLSLAGNKEASNQCIQNKII
                     DVQLSGAQFISTACPACFNQFDLGQFIASRENPQLKENPIPVLYAIELLALAMGRSFE
                     EIGYNLHRIKTPVKEKV"
     gene            1830..3905
                     /gene="fdhF"
                     /locus_tag="PKJ95_01145"
     CDS             1830..3905
                     /gene="fdhF"
                     /locus_tag="PKJ95_01145"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNR64878.1"
                     /translation="MEKVKTTCVYCGTGCQLWLKTLNGKVVDTRPVMPPEFNPGKGKL
                     CIKGWNVHEFINHPDRLTDPLVKSENGKWEKVSWEEAIDYIANYIKRVMSENPDNRRI
                     IGCFSSAKCTNEENYVMQKFARAVLKTNNIDHCARLCHSATVVGLGAAFGSGAMTNSI
                     DEIPDAQVIFVIGSNTNEQHPLIGTRVLEALKKGGKLIVADPRTIPLSELAEDNGGVA
                     LQHRPGTDVALLNGIMNVIINEKLYDHDFVTQNCENFDEFKEEILKMSPERASHITGV
                     PQDKIIRAARMIGNADTTYLIYSMGITQHTSGVDNVKSTANLQMLCGNVGKWATGVNP
                     LRGQNNVQGACDMGALPNLFTGYQSVTDPVIRNKFALEWGIEEKDMDDQIGQTVVEMI
                     DAAYQGELKMLYIMGENPVMSDPDSNHVRAALQKTFLIVQDIFMTPTAELARVILPAV
                     SFAEKEGTFTSTERRVQMVRKAIEPVGNSKPDWQIISMIAQKMGYSGLNYKNVEEILT
                     EINRTTPIYGGITWERLEQNEKGLQWPCPDISHPGTVFLHKEGKFSRGKGYFTPILFK
                     EPAELPDSDYPLVLSTGRTLWHFHTGTMSRRSKTLNERVPEGYVEISQEDASKLKISD
                     GEMVKVITRRGEIEIKARVGHKVKEGLIFIPFHFEEAAANILTNAAVDPVAKIPEFKV
                     CAARIEKII"
     gene            4002..4586
                     /locus_tag="PKJ95_01150"
     CDS             4002..4586
                     /locus_tag="PKJ95_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNR64879.1"
                     /translation="MTVKVVGIIGSPRKGMNTDTLVSRALEGAKSSGADTEKIYLNDL
                     KILPCQACTRFPAPDYCFFHDGMDKIYQALLNADALVIGAPAYFGSMSAQLKLLIDRS
                     NCLAEMIALPDGKVRFISRLEKRKKGIFIWVANLSKNPEPALVSIRLWSKYFANVELL
                     DTLLATESDLGEGARNKKEYLEQAFNLGVLLVQP"
     gene            4819..>5636
                     /locus_tag="PKJ95_01155"
     CDS             4819..>5636
                     /locus_tag="PKJ95_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64880.1"
                     /translation="MFRAKVKKIYTWVLTLFLISFLSNPCLAAISVQEIINNMQKAYD
                     KQMAGINDYMVIQKPSGGLGAIAGETKMYYKKAKVDGEEIYKTRTETEVMGMKMVSIY
                     DGKYSWSTNPLTGKVESKIAEGDPLQFWNWKSIDSTKTEYLGEEMIDGEKTYILRIDN
                     AMQVMSSQAQAAAMQENRPQGATGKLWLSSKTWMPLRMEMIIKSDSEGMAMNIITTTD
                     FEDYREVGTIIHPFKIIANTSTEIDTSGVSEDVKEEGEEMWEMMQSMMSEMGSFT"
BASE COUNT         1925 a          907 c         1215 g         1589 t
ORIGIN      
        1 taggattggt gacattgggg tatatctgaa tgcacaacag caaagtctac tatccccatg
       61 gtttaaatac ttccttactt atgacccagg accagcatta agtatggtta aatgccccgt
      121 gctggcttta tatggtggga aagacctgca ggtaccatct gtggaaaacc ttaaagcaac
      181 agaaagtgct ttaataaaag gaggcaataa ggattttacg gtaaaggaat tttctggtct
      241 aaatcatctt ttccagactg cccaaacagg ttcgcctgat gaatatggcc gtattgaaga
      301 aaccatttca ccccgtgtgt tagaatttat cagtgattgg atatggagac atgtagaaaa
      361 ttgatttaaa atataatatt ctagattaaa taagtataag attacactgc caccgatggt
      421 aaaaacaatt attttctata ataggagatg taaatgaagg tattacgctt acaatatgaa
      481 aaaaatgaaa ttttgaaaaa aaataaagca attgaggttt gttttaaatg ttctacctgt
      541 tctaaatttt gccctattac tcaatatgtg aagaaatata atcaggaaaa ctcatttgta
      601 acacatcttt ttgaagcaga aaaatcagag gctctaaacg atgtctggat gtgttgtgcc
      661 tgcgaaaagt gtgttatcac ctgtcctcag gatactaatc ccacagatgc ctttaccaat
      721 ttaaaggaaa aatcttaccg ggaaggatat gctccggaga tagtctatcg tttgatggaa
      781 caactattac aggaggggac agtttacaat attaaattta gcaatgccgc acgaaaacga
      841 gctggattga cagaaataga gaaaaatgaa agagccatta aagaattgaa catattagcc
      901 gataaaactg gtttagtgag aggaaagggt aataactaat gataaattat ttatttttta
      961 aaggatgtac tattccggct aatttgccaa atattgaaaa actaacttta gagattttac
     1021 ctgaaatagg cattaatttg tcggtgagtg atgaatttac ctgttgtcct gaccctataa
     1081 gacttcaggg agccaatcag tatttctgga tggtttcagc tgccagaaat attaccattg
     1141 cggaggaaaa aaatatgaat attatgacct tgtgcaatgg gtgtgaaaat actctgggaa
     1201 ttgtaaatta taaactgaaa aataatcccg aacttaaaag caaggtaaat gaggtattaa
     1261 aagatattgg cagagagtta aagagtatta ttgaggtaaa acatttttta caggtcttaa
     1321 aagaagatat cggattggat aatcttaaac agatgattaa gaatcctttg agcggtttta
     1381 agattgctgg ccatcccgga tgtcatcttc tgatgcccaa tgaaatacta aaatttgata
     1441 accccgtaga tccggagttt tatgaccaat tcattgctgc tctaggagct actccaattg
     1501 actatctatc aaagattgat tgctgtggcg tatctctcag tctggctggt aacaaagagg
     1561 cttccaatca atgtattcaa aataaaatca ttgatgttca gctttccggg gcgcaattta
     1621 tcagtactgc ctgcccggct tgttttaatc agtttgatct ggggcaattt attgcttccc
     1681 gggaaaaccc gcagttaaaa gaaaatccca ttccagtttt gtatgctatt gaacttttag
     1741 ctttagctat gggtcgctct tttgaggaaa ttggttacaa tctgcatcgt ataaaaactc
     1801 cggttaagga aaaggtttag gaggaagaaa tggaaaaagt caaaactacc tgtgtatatt
     1861 gtggaaccgg ctgccaatta tggctaaaaa ctttaaatgg aaaagtggtg gataccagac
     1921 cagttatgcc tcctgaattt aaccccggca aaggtaagtt atgtattaag ggatggaatg
     1981 tacatgaatt tattaaccac ccggaccgct taacagatcc tcttgtcaaa agtgaaaacg
     2041 gaaaatggga aaaagtaagc tgggaagagg ctatagatta tattgcaaat tatatcaaaa
     2101 gagtaatgag tgaaaatccg gataacagaa gaatcatagg atgttttagt tcggctaaat
     2161 gtaccaacga agagaattat gttatgcaga aatttgcccg agcagtttta aagaccaaca
     2221 atattgacca ttgtgcccga ttgtgtcatt ctgccacagt agtaggcctg ggagcggctt
     2281 ttggttctgg tgctatgact aactctattg atgaaatacc cgatgcccag gttatttttg
     2341 taattggttc aaataccaat gagcagcatc ctttaatcgg gaccagggta ttagaggcac
     2401 tgaaaaaggg aggaaaattg atagttgctg acccccgaac tataccatta tcggaattag
     2461 cggaagataa tgggggagtt gccctacaac accgtcctgg taccgatgtg gctttattga
     2521 atggaataat gaatgtgata atcaatgaga aactctacga ccatgatttt gttactcaaa
     2581 attgtgaaaa cttcgatgaa tttaaagaag aaatattaaa aatgtctcct gaaagggcct
     2641 ctcacatcac cggtgtgcct caagacaaaa taattagggc tgctcgcatg ataggcaatg
     2701 ccgatacgac ctatttaatt tattcaatgg gaattaccca gcataccagc ggagttgaca
     2761 atgtgaaaag tactgctaat ttacagatgc tatgcggaaa tgttggcaaa tgggcaacgg
     2821 gggtaaaccc gctacgaggt cagaataatg ttcaaggtgc ctgtgatatg ggtgccttgc
     2881 ccaatctctt taccggatat caatcggtaa cagaccctgt aatacgtaat aaatttgcct
     2941 tagaatgggg tattgaagaa aaagatatgg atgatcaaat tgggcaaaca gtggtggaaa
     3001 tgattgatgc agcatatcag ggagagttaa aaatgcttta tataatgggt gagaatcccg
     3061 tgatgagcga cccggacagt aatcatgtta gagctgcttt acaaaaaaca tttttgattg
     3121 tacaggatat ttttatgact cccactgctg aattggccag agtaatttta ccggcagttt
     3181 ctttcgcaga aaaagagggc acttttacca gtacagaaag aagggtgcag atggtgagaa
     3241 aggccataga gccagtgggc aattccaaac cggactggca aatcatttct atgatagcac
     3301 agaaaatggg atattcaggt cttaactata aaaatgtaga agaaatatta acggaaataa
     3361 atcgtactac tcctatatat ggcggaatta cctgggaacg actggaacaa aacgaaaaag
     3421 gcttgcagtg gccttgtccc gatatatctc accccggaac tgttttttta cataaagagg
     3481 gaaaattcag cagaggtaaa ggttatttta ccccgattct ttttaaagag ccggcagaat
     3541 tacccgactc agattatcca ttggtactct ctacagggag aaccttgtgg catttccata
     3601 ctggaactat gagtagaaga tctaagacat taaatgaaag agttccggaa ggatatgtgg
     3661 aaatctccca ggaagatgcc agcaaactaa aaatcagtga tggtgaaatg gtaaaagtaa
     3721 taaccaggag aggggagatt gaaattaaag cgagagtggg tcataaggtt aaagaaggtt
     3781 taatatttat tccttttcat tttgaagaag cagcagcaaa tatattaaca aatgcagccg
     3841 tggaccctgt tgccaaaatt cctgaattta aggtttgcgc tgccagaatt gaaaaaataa
     3901 tataatattt gtctttaaaa aacagaatta tcattacata agtgaataaa tcaaaatttg
     3961 tgcgaatttt gtttattaat tgataaggag gaagttccta tatgacagta aaagtagttg
     4021 gaattattgg aagtcctcga aagggaatga ataccgatac tctggtgagc agagcactgg
     4081 aaggtgctaa atcatcagga gcagatactg aaaaaatata cctgaatgat cttaagattt
     4141 taccctgtca ggcttgtacc agattccctg ctccggatta ttgctttttt cacgatggga
     4201 tggacaaaat ttaccaggct ctgctaaatg ctgatgcttt agttatcggt gccccggcct
     4261 attttggttc tatgagtgca caactcaagt tactcattga tcgttccaat tgcctggcgg
     4321 aaatgattgc acttcccgat ggcaaagtaa ggtttatatc aaggctggag aaaagaaaaa
     4381 aaggaatatt tatctgggtg gctaatttat ctaaaaaccc tgaacccgcg ctggtttcca
     4441 ttagactctg gagtaaatat tttgccaatg ttgaattgtt agatacccta ttagctactg
     4501 aatctgatct tggggaggga gccaggaata aaaaagaata tctcgaacag gctttcaatt
     4561 taggcgtgtt gttagtacag ccataaactt tttatgttta taacagataa atctatcagg
     4621 taactatttg ctgtaacttc agctatttaa tttttctata gaagcaaaaa caaaaatata
     4681 ttgattaaca aaaaagaagg ttatataatt aagaaaaacc actaatatac taatatataa
     4741 tagttcttag tttttacagt atcagtcaaa attctttctg tttttataaa tagttgttaa
     4801 aatttaaagg agggttaaat gtttagagca aaggtaaaaa agatctatac ctgggtttta
     4861 actttatttc tgatatcatt tttatccaac ccctgcctgg cagcaataag tgtgcaggaa
     4921 atcatcaata atatgcaaaa ggcttatgac aagcagatgg ctggaattaa tgattacatg
     4981 gttattcaga aaccgagcgg aggactaggt gccattgctg gtgaaacaaa aatgtattac
     5041 aaaaaagcaa aggtggacgg agaagagatt tataagaccc gtactgaaac agaagttatg
     5101 ggaatgaaaa tggtttctat ttacgatgga aagtatagct ggtcaactaa cccgcttacc
     5161 gggaaagttg aaagtaaaat agcagaggga gacccattac agttctggaa ctggaagagt
     5221 attgattcaa ccaaaactga atatttggga gaagaaatga ttgatggaga aaaaacctac
     5281 atattacgga ttgataacgc tatgcaggtt atgagttcgc aggcacaagc tgcggcaatg
     5341 caagaaaata ggcctcaggg agctaccgga aagttatggc ttagcagcaa aacctggatg
     5401 cctctacgta tggagatgat tataaaatct gattcagaag gaatggctat gaatatcatt
     5461 actacaactg acttcgaaga ttaccgagag gttggaacta taattcatcc ctttaagata
     5521 atcgcaaata cctccacaga aatagatacc tctggagtga gtgaggacgt aaaagaagaa
     5581 ggggaagaaa tgtgggagat gatgcaatct atgatgtctg aaatgggctc attcac
//
LOCUS       DAOQWT010000028         5585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_14801_length_5585_cov_3.559855, whole genome shotgun sequence.
ACCESSION   DAOQWT010000028 DAOQWT010000000
VERSION     DAOQWT010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14801_length_5585_cov_3.559855"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PKJ95_01160"
     CDS             <1..900
                     /locus_tag="PKJ95_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64881.1"
                     /translation="LWPFRFKSKLLNRLLAVLYLKQGLCSMKVLAIDYDGVIVDSVLD
                     SLFVSHNAYLKLFGNEKRKQFGGKPFTFQNWPTILQKYSKEIEYYRSLRPYIRDAIDY
                     GLIQKLMENGKNIQSQIEFDKYRKTDRFGLEKFYDAFFKERKKLQEEDFEGWLSLEPP
                     YEEAIKGIRKFVKEGIKVMVATSNRTEFIFQVFHPLYYDIPVKYEDILDFNFGEDKSK
                     QIQYIHEKFKVNLKDIYFMDDQLAHLEQTRSLGISVFLAGWSYATIQQKGRAKIEGIP
                     VINRSEDFYSILKEHMNRRLKYH"
     gene            934..1272
                     /locus_tag="PKJ95_01165"
     CDS             934..1272
                     /locus_tag="PKJ95_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR64882.1"
                     /translation="MKVIDYHKVPLEDMEMEGAKNVKVRWLISDKDNAKNFAMRLFEI
                     GEGGFTPYHTHDWEHEVFVLEGEGEVKIEEKVYPIKKDSVVLVDPRIIHGFRNTGKDM
                     LKFLCLIPYK"
     gene            1327..2154
                     /locus_tag="PKJ95_01170"
     CDS             1327..2154
                     /locus_tag="PKJ95_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNR64883.1"
                     /translation="MKILAVDFDGVISDSALKSLFVGHNVYCRHFGTNAKKSFGGKCF
                     TFENWEHLKKEYHQEMEKYKRLRAYVEQSCDFLVMIKIIEEQIEVDNQQDFIRIRNEM
                     DLDYRFFHELFFKEKEKWQKKDFAKWFSLAPVYKKVVEGIKRLLDEGVKVVIATSNLG
                     KAIHPAFHADYLGFSMELKDIFDKNYGKSKSDHMKAIVEQYKVAHKDIYFVDDQLNYL
                     EETKVFGVNVFLAGWGYCTEEQKSIARKEDITVIESEEEFYPTMKLNFTGDGSLQNS"
     gene            complement(2546..3133)
                     /locus_tag="PKJ95_01175"
     CDS             complement(2546..3133)
                     /locus_tag="PKJ95_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64884.1"
                     /translation="MKRNKFLPVIFLIFSLIMLLVFSVTIIASEQDLIKESLSFLEKQ
                     DYQSAAHLLEEALSIIWNKSPLELNNLIFCEEKPDGFGFYQKRIDYNFTEGDTFCLYA
                     EPKYYSIVKIEENLFEVHFKGDLYILDTEGALLWGQRDLWDYHIISNAPNKEIFITYS
                     DSFLNFPEGEYQFQLVLKDVASQKTVEQAISFVIE"
     gene            complement(3344..3892)
                     /gene="lepB"
                     /locus_tag="PKJ95_01180"
     CDS             complement(3344..3892)
                     /gene="lepB"
                     /locus_tag="PKJ95_01180"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNR64885.1"
                     /translation="MAKINTVLNKKNGKKVKPMWRELIETVVSAGIIAFIIITFIGQV
                     TVVKGASMEDTLFNRERLICNKIVYRLTEPKHGDIVIFKPPIDQNHNYIKRVIAVEGD
                     RVKIVDGVVYLNGEKIEEDYITHHSSETMPEITVRENSFFVLGDNRVNSSDSRFWGFV
                     PRKNIIGRASVIFWPINRARIP"
     gene            4073..4741
                     /locus_tag="PKJ95_01185"
     CDS             4073..4741
                     /locus_tag="PKJ95_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNR64886.1"
                     /translation="MFDLDDTLIKTSKIYNNARSEFSKVMSQLGFPVDEALEKLDEID
                     IGHINEQGFSKERYPLSLVKTYHHFCHRFGKKIYKKTEKEIGNIGRDVFQQIPEPVEG
                     VDLVLNNLKERYFLILATLGDPEIQHRKINHSGLKDYFQAIYVLNYKTADEYLQILVK
                     HNLNREKTWIIGNSIRSDLNPGLQLGLNCILIPAVTWVFEEEKPISEHYLKLNSLVEV
                     LKHL"
     gene            5156..>5585
                     /locus_tag="PKJ95_01190"
     CDS             5156..>5585
                     /locus_tag="PKJ95_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR64887.1"
                     /translation="MKKYPSYLNISVEEFEKRIKDAEQILQSCRICPHQCGVNRIAGE
                     KGLCRSTAQVMVSSYNAHFGEEPPISGIGGSGTIFFTNCTLRCVFCQNYPISQLGNGN
                     PVSISQLSKMMLDLQKRGCHNINLVTPTHFVPQIIAALGQA"
BASE COUNT         1884 a          788 c         1123 g         1790 t
ORIGIN      
        1 ctttggcctt ttcgtttcaa aagcaagcta ttgaacaggt tattggctgt tctttacctg
       61 aagcaaggac tgtgtagtat gaaggtattg gctatagatt atgatggtgt gattgttgat
      121 agtgtgttgg attctctttt tgtcagtcat aacgcctatt taaaattatt tggtaacgaa
      181 aaaagaaaac aatttggagg taaaccgttt actttccaaa attggcccac aatattacaa
      241 aaatatagta aagagataga atattaccgc tcgttaaggc cttatatccg tgatgctatt
      301 gattatgggc tgatacaaaa attgatggaa aatggaaaaa atattcaaag ccagatagag
      361 tttgataaat acaggaaaac agaccgtttt ggcttggaaa aattttatga tgcttttttt
      421 aaagaacgga aaaaactgca agaagaagat tttgaaggat ggctaagttt agaaccaccc
      481 tatgaagagg caatcaaagg gatacgtaaa tttgtaaaag agggaattaa agtcatggta
      541 gctacttcca acaggacaga atttattttt caagtatttc atcccctcta ttatgatatc
      601 ccggttaagt atgaagatat ccttgatttt aattttggag aagataaatc taaacagatt
      661 cagtatattc atgagaaatt caaagtaaat ttaaaggata tttactttat ggatgaccag
      721 ctggcacatt tagaacagac ccgtagcctg ggtatatctg tattcttagc tggttggagt
      781 tatgccacca ttcagcagaa gggaagagca aaaattgagg ggataccggt gattaacagg
      841 tccgaagatt tctattccat attgaaagaa catatgaata gaagattaaa atatcattaa
      901 tcaaatatga agtataaagg gaggagaaat aagatgaagg ttattgatta ccataaagtg
      961 cctcttgaag atatggagat ggaaggggca aaaaatgtaa aggtaaggtg gcttatcagt
     1021 gataaagata atgccaaaaa ttttgccatg agattatttg aaattggtga gggaggtttc
     1081 accccatatc atactcacga ttgggagcat gaagtatttg tgttggaagg agagggggag
     1141 gttaagatag aagagaaggt ctatcctatt aaaaaagaca gtgtagttct agtagatccc
     1201 cgaattatac atggctttcg aaacaccggg aaagatatgt taaaattcct ctgtttaatc
     1261 ccttataaat aattgcacat ctctaataat aattttgttg aagattatga ttgttgagga
     1321 ttatctatga agatactggc agttgatttt gatggagtaa tcagtgacag cgcccttaaa
     1381 tcattgtttg ttggtcacaa tgtctattgc cgccattttg ggacgaatgc aaaaaaaagt
     1441 tttggaggta aatgtttcac atttgagaac tgggagcatt tgaaaaagga ataccatcaa
     1501 gagatggaaa aatataaaag attgagggca tatgtggaac aatcctgtga ttttttggtt
     1561 atgataaaaa ttattgaaga gcaaatagag gtggataacc agcaggattt tatcagaatt
     1621 cgcaatgaaa tggacttaga ttaccgtttc tttcatgaat tattctttaa ggaaaaagaa
     1681 aaatggcaga agaaagattt tgcaaaatgg ttttccctgg cacctgttta taaaaaggtg
     1741 gttgagggaa ttaaaagatt attggatgaa ggagttaagg ttgtaattgc cacctccaat
     1801 ttgggcaagg caatacaccc ggctttccat gctgattatc tgggattctc tatggagtta
     1861 aaagatattt ttgataaaaa ttatggtaag agcaaatctg accatatgaa agctatagtg
     1921 gaacaatata aggtcgccca taaggatatt tactttgttg atgaccagtt gaattatctg
     1981 gaagaaacga aagtttttgg ggtaaatgtt tttttagcag gctggggata ttgtacagag
     2041 gaacaaaaga gtattgcccg aaaagaagat ataactgtta ttgaaagtga agaagagttt
     2101 tatcctacta tgaaactaaa ttttacaggg gacggttctc tgcaaaatag ttaaaagtta
     2161 gtagttattg gtttatagat tttggaataa aagatgtggg cataaatgta ggaggtttgt
     2221 agttaaatca ttgcgggtat aagagtaagc ggttcttagt taatggttta tagtttttaa
     2281 taaaagaaga agatgtaggc ataagtgtgg gaagagaaat gcaaagatga ggatggaagt
     2341 gtaagaaaaa gagtgcaaag agggttgtag gaataaaaat acaaagatgt gggtgataga
     2401 gtatgagatt gccacgacat actttcagta tgtctcgcaa tgacaagaca aagaaaaggt
     2461 ttcagtcaaa caggagacgg ttctctgttt gactcctaag aaatctgtaa aacagaaaac
     2521 cgtccctttt ttgtgggttt ttacattatt caattacaaa actgatcgct tgttcaactg
     2581 ttttttggct ggctacgtct tttaaaacga gttggaattg atattcacct tcgggaaaat
     2641 tcagaaaaga atcactgtag gtaataaaaa tttctttatt gggtgcattt gagataatgt
     2701 ggtaatccca gagatctctt tgtccccaga gaagagctcc ttccgtatct aatatatata
     2761 aatcgccctt gaaatgaact tcgaaaagat tttcttctat tttcacgatg gaataatatt
     2821 ttggttcggc atataggcaa aatgtgtccc cttctgtaaa attatagtca atacgcttct
     2881 gataaaatcc aaatccatca ggtttttctt cgcagaaaat gaggttattc aattctaaag
     2941 gggatttatt ccagatgatt gataatgctt cttccaatag atgtgcagct gattgataat
     3001 cctgtttttc caaaaaagac agggactctt taatcaggtc ttgctctgag gcgatgatag
     3061 tgaccgaaaa tacgagaagc ataattaaag aaaaaattag aaagataaca ggtaaaaatt
     3121 tatttctttt cataaaatct cctttaaatt aaaataatat tttttaacaa ccaaaaaaac
     3181 agttcttttt atgttaaatg aacaaatcat aaaataaata ctcattgctg ataaactatc
     3241 ctatgttatt gccatctctt tgtttgcatt tttactaaat actaaattat cgactaatga
     3301 ttattttagc aatctcaccc actgcttctt aaaaaattaa aagttaaggt atcctagccc
     3361 tgttaatagg ccaaaagatt actgaagctc taccaattat attttttcgg gggacaaagc
     3421 cccagaagcg gctatcacta ctgttaaccc gattatcacc aagaacgaaa aacgaatttt
     3481 cccgaactgt aatttcaggc atcgtttcag aggaatgatg tgttatataa tcttcttcta
     3541 ttttttctcc attaagataa acaactccat ccactatctt aactctatct ccttctacag
     3601 caatgaccct tttgatgtaa ttatgattct gatcaatagg tggtttaaaa attactatat
     3661 cgccatgctt cggttcggtt agccgataga cgatcttatt acaaattaat ctttcccggt
     3721 taaacaaagt atcttccata gaagcgcctt taaccactgt aacctgtccg ataaaggtta
     3781 tgataataaa ggctattata ccggcactaa ctactgtttc aataagttca cgccacattg
     3841 gttttacctt tttcccattt ttcttattaa gaactgtgtt aatttttgcc atatagtcct
     3901 ctttccatat ttcttttcca aaaaattgtc atcattcaga attataccat atttctataa
     3961 atttcaagca gcaaaaaaat tttactcaaa atgacaattc aggttataat taaaaacagt
     4021 tttgtaatat ttgttttact aaagtgagga gggaaaatag atcagtacta ttatatttga
     4081 tctagatgat accttaataa agacctctaa gatttataat aatgccaggt ctgaattttc
     4141 taaagtgatg tcccaattgg gatttccagt tgatgaggct ctggaaaaac tcgatgagat
     4201 tgatataggg catatcaacg aacaagggtt ttccaaagag agatatccct tgagtctggt
     4261 gaagacctat catcattttt gccatagatt tggtaagaaa atttacaaaa aaacagaaaa
     4321 agagattggc aatattggca gagatgtttt tcaacagatt cccgagccgg ttgaaggagt
     4381 agatttagta ttaaataatc ttaaggaacg ctatttttta attttagcca cactgggaga
     4441 cccggaaata cagcatcgca aaatcaatca ttctggctta aaagattatt ttcaagccat
     4501 atatgtcctt aattataaga cagccgatga gtatttacag attttagtga aacataatct
     4561 aaatagggag aaaacctgga ttattggcaa cagtatcaga tctgacctga atccgggact
     4621 gcagctggga ttaaattgca tactgattcc cgcagttacc tgggtatttg aagaagaaaa
     4681 gcctatttct gaacattatt taaaattaaa ttctctggtc gaagtgttga aacatttgta
     4741 aaaataaagt ttttagttta tagtttttgg tttttagaaa attttacagg ggacggttct
     4801 ctgcaaaata gttaaaagtt agtagttatt ggtttattaa gtttttggtt aatagttttt
     4861 gttaaatcat tgtgggtata aatggaagta gtcgttggtc gttagtagtt ggtatttggt
     4921 gaaaaagaat gtgagtataa gtgaaagtag tcgttggtcg ttagtagttg gtatttagtg
     4981 aataaagatg tgggagtaag tgaagggggt tcatggttaa tggttttaag tttttagtaa
     5041 aagacaaata caaagatgtg gggagaaata aaagtggtcg tttgttgttg gtatttagtt
     5101 ggtatttaat attttaagtt catgtttttg aatttatagc aatagaaaaa ggaagatgaa
     5161 aaaatatcca tcgtatttaa atattagtgt agaggagttt gaaaaacgta ttaaagatgc
     5221 agagcagatt ctacaatctt gccgaatctg tccacatcaa tgtggagtaa atcgtattgc
     5281 cggtgaaaaa ggtctttgcc gctctaccgc tcaggttatg gtatcaagct ataacgcgca
     5341 tttcggagaa gaacccccca tttctggaat tggtggctca ggcactatct tttttactaa
     5401 ttgtactctg cgctgtgttt tttgtcagaa ttatcctatc agtcagctgg gtaatggtaa
     5461 tcctgtgtct atctcccaat tatctaaaat gatgctcgat ttacaaaaga ggggttgtca
     5521 taatattaac ctggttaccc cgactcattt tgtgccacaa attattgcag cgctcgggca
     5581 agcca
//
LOCUS       DAOQWT010000029         5508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_15059_length_5508_cov_3.021823, whole genome shotgun sequence.
ACCESSION   DAOQWT010000029 DAOQWT010000000
VERSION     DAOQWT010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15059_length_5508_cov_3.021823"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKJ95_01195"
     CDS             complement(<1..415)
                     /locus_tag="PKJ95_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR64888.1"
                     /translation="MEDFTPSEIVAELDKYIVGQKQAKEAVAIALRNRIRRKKLPKAL
                     MEEITPKNIIMIGPTGVGKTEIARRLAKLVQAPFIKVEATKFTEVGYVGKDVDSMIRD
                     LTQAAVQLIRKEKIGEVREKAKERVEERILQALLPI"
     gene            complement(432..965)
                     /gene="hslV"
                     /locus_tag="PKJ95_01200"
     CDS             complement(432..965)
                     /gene="hslV"
                     /locus_tag="PKJ95_01200"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNR64889.1"
                     /translation="MYKGTTILAVRRNNEIAIAGDGQITMGNIIAKNEAKKIRKIYHN
                     TILTGFAGGAADALALYERFEIKLEEFQGNLKRASIELAKDWRTDKVLRRLEALLIVA
                     DLQDMLTISGNGDIIEPDKPVAAIGSGGPYALASAQALLSFTQLSATEIALESLKITS
                     SICIYTNKNITVETLKG"
     gene            complement(1037..1957)
                     /locus_tag="PKJ95_01205"
     CDS             complement(1037..1957)
                     /locus_tag="PKJ95_01205"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HNR64890.1"
                     /translation="MSNYINNSNIIKEYHKYLKDKKHFPDNTKNAYLKDIISLSKFIE
                     NNKEITSLTDVSHQMLRSYVVYLKQKSFSNKTIARKISSFRAFYRYLIQEELVDKNPA
                     QYVQIPRIQKKLPDFLFIEEVVRLIDSLKTDKPIGARNKAILELLYGTGIRVAELSNL
                     DIDDFNLDDDTIKILGKGSKERILPLSKPVKNAVKNYLKVRKLIPRKKYLKMLSEKAM
                     FVNCWGERLSTRSVRMIINNNIQLACLNKKISQHIFRHTFATHLLSGGADLRSVQELL
                     GHESLSTTQIYTHITKDKLIEIYRQSIPRK"
     gene            complement(1950..4058)
                     /gene="topA"
                     /locus_tag="PKJ95_01210"
     CDS             complement(1950..4058)
                     /gene="topA"
                     /locus_tag="PKJ95_01210"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNR64891.1"
                     /translation="MKKDLIIVESPTKERVLKEMIGDKFLIISSKGHIKDLPESRMGI
                     DIENNFKPTWLIIPSKRQIVKNIKNIAQGKDKILLATDPDREGEAISWHLAEELDIGN
                     QKCRISFNEISKNVVIQAIENPRKLDMHIINSQIARRLLDRLVGYKVSPLCYKYARGK
                     SAGRVQSVAVHLIVEREKEIEAFKSEEYWKINVFLLKEKDSEDKVFQATLISKNDIKI
                     KINNQKEADFYCEDIKECPFIVSKITISEEYKKSPLPLKTSTLQQIAFHKLNFSVKKT
                     MRVAQQLYEGISITGTGTLGLITYMRTDSTRLSGEAIINARKYILEKYGKEYQTFDKR
                     ILKKSKNKEPLIKIQDAHEAIRPTSINLEPLSIKDDLTNDQFRLYQLIWEYFIASQMK
                     PALIEKKNVRIGAKQYVFEANGSCIKFPGYLKVLKENEIEKVILPDLKEGEKLTLLNI
                     DPRQSFTKPPARYNEASLVKVMEKEGIGRPSTYSVIIDKILSRGYVEKENKKLLPTKL
                     GIIVDQFLTNYFSNIINVKFTAKMEQELDKIESGEHNWQNVLNDFYKSLLLSINRLTE
                     KPPQKLMPEFKELVDEKCMVCGNSMEIKNGPYGKYLACSQFPLKHPTKPFLFKIGIAC
                     PKEGCSGEIIKLKSKKGRFFYGCSNYPNCDFCSNFIPVDKKCPDCGSILVKRNIRGKG
                     SFYQCFNKECHYHEKVLKNE"
     gene            complement(4153..4779)
                     /gene="plsY"
                     /locus_tag="PKJ95_01215"
     CDS             complement(4153..4779)
                     /gene="plsY"
                     /locus_tag="PKJ95_01215"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNR64892.1"
                     /translation="MEIIMLARTILLLIISFFLGSIPFGYLVSKYLKKIDIREYGSGN
                     IGTANTFRVLGIRYALLVLIGDCLKGLLAVYLVKLITTESITLQLLTGFFAIMGHNWS
                     IFLKFKGGKGIATTFGVILSIYPNIAFISAIIWVALVIVFRFAALGSIVSVFSMLILS
                     FIFETPVEFKYFLIVVNLLAIFRHRSNIVRLLQHRENRIISNRDYLKK"
     gene            complement(4795..>5508)
                     /locus_tag="PKJ95_01220"
     CDS             complement(4795..>5508)
                     /locus_tag="PKJ95_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNR64893.1"
                     /translation="IVDESPGTTRDAIEVSLYFDEYKIKFIDTAGLRRKKNVKQKIEY
                     FGNLRTIDSIKKADIVLLILDATHIISMQDKRLANIIIEEKKACIVLLNKFDLVKKDI
                     GIDKNQLLKISKNSLKFLKDAPIMTTIALNPTEVYLPVLKTIIEMFRKYNRKISTSEL
                     NNFLQNIVNKRAPKIVSGKKIHFYYITQVKIKPPTFKIFVNNPQLLYNSYQRYMENQF
                     VKNFDFKGIPILFSYEKRK"
BASE COUNT         1613 a          958 c          796 g         2141 t
ORIGIN      
        1 tgatgggaag caatgcttgt aatattctct cttcaactcg ttctttagct ttttcccgta
       61 cttcaccaat tttttctttg cgaataagtt gcactgcagc ctgtgttaaa tctcttatca
      121 tcgaatctac atctttccct acataaccga cttcggtaaa cttagtagcc tcaactttaa
      181 taaatggtgc ctggaccagt tttgccaatc ttcgtgcgat ttctgtttta cctaccccgg
      241 tagggccaat cataataata ttttttgggg tgatttcttc cattaaggct ttaggtagtt
      301 tttttcttct aattctattt cttaaggcaa ttgctacagc ctcttttgct tgtttttgtc
      361 caacaatata tttatctaat tctgctacta tttcagatgg tgtaaaatct tccaattcta
      421 tacccccttc attatccttt taaggtttca acagtgatat ttttattagt atagatacaa
      481 attgaggaag taatttttaa tgactccaaa gcaatttcag tggcagataa ttgagtaaag
      541 gaaagtaaag cctgagcaga ggctaaagca tatgggcctc ctgaaccgat agcggcaaca
      601 ggtttgtctg gctctataat atcgccatta ccggagatgg taagcatatc ctgcaaatcg
      661 gcaactatta gtaaagcttc taatctcctt agaactttat ctgttctcca gtctttagca
      721 agctcaatag atgcccgttt taaattaccc tgaaattctt ctaatttaat ttcaaatctt
      781 tcatataagg ccagtgcatc tgctgcgcca ccggcaaaac ctgttaatat agtgttatgg
      841 tatatttttc tgattttttt tgcctcattt tttgcaatta tattccccat ggtaatttgt
      901 ccatcccctg caatagcgat ttcattattt ctcctaacag caagaatagt ggttccctta
      961 tacataccat taattccttc tttaatacct atatttttta aatatctttg ttattatttt
     1021 tttaagatat tgtcaatcat tttctgggaa tcgattgtct ataaatttct atcaatttat
     1081 ccttagtgat atgagtataa atctgagtgg ttgataaact ttcgtgcccc aataactcct
     1141 gtactgaacg taaatcagct cccccactta atagatgagt agcaaaggtg tgacgaaaaa
     1201 tatgttggct tatctttttg tttaaacaag ccaactgtat attattatta attatcattc
     1261 taacacttct ggtagaaagt ctttctcccc aacaattaac aaacattgct ttttctgaaa
     1321 gcatttttaa atattttttt ctgggtatca atttccttac ctttaaataa ttttttacag
     1381 cattttttac cggtttgctt agcggtaaaa tcctttcttt tgaaccctta cctaaaattt
     1441 ttatggtatc atcatccaga ttgaaatcat caatatcaag attggatagt tctgctactc
     1501 ttattccggt accatataat aattctaaga tagctttatt tctggcaccg ataggtttat
     1561 ctgtttttag agaatctatc aatcttacta cttcttcaat aaacaggaaa tcaggtaatt
     1621 ttttttgaat tcttggaatt tgtacgtatt gagcaggatt tttgtcaact aactcctcct
     1681 gaattaaata tcgataaaaa gcacgaaatg aggagatttt tctggcaatg gttttattgc
     1741 taaaactttt ctgttttaaa tatactacat agcttctgag catttggtgg ctcacatcag
     1801 ttagactggt tatctcttta ttgttttcta taaacttaga taagctgatg atatctttta
     1861 gataagcatt tttggtgtta tcaggaaagt gctttttatc ttttaaatat ttatggtatt
     1921 cttttataat gtttgaatta tttatatagt tactcatttt ttaagacttt ttcatgatag
     1981 tggcattctt tgttgaaaca ctggtaaaag ctaccctttc ctctgatatt tctttttacc
     2041 agaatcgaac cacaatccgg acattttttg tctactggaa tgaaatttga gcaaaaatca
     2101 caattaggat aattgctgca accataaaaa aaacgacctt ttttactttt taacttaatg
     2161 atttcaccgc tacatccttc ttttgggcaa gcaataccta ttttaaagag aaaaggttta
     2221 gtaggatgtt ttaacggaaa ttgggaacaa gccaaatatt taccatatgg accatttttt
     2281 atctccattg aattaccaca taccatacat ttctcatcca ctaattcctt aaattctggc
     2341 attaatttct gaggtggttt ttctgttaga cggtttatac ttaacagtag tgatttataa
     2401 aagtcattga gaacgttttg ccaattgtgt tctccagatt ctattttatc cagttcttgc
     2461 tccatttttg cagtaaattt tacatttatt atattagaaa aataatttgt taaaaattga
     2521 tcaacaataa ttcctaattt agtaggtagc aattttttat tttctttttc aacataacct
     2581 ctgcttaata ttttatcaat tataactgaa taggtgcttg gcctgcctat gccttctttc
     2641 tccattactt ttactaaact tgcttcatta tatctagcag gtggtttagt aaaggattgt
     2701 ctggggtcaa tatttaataa tgtcagtttt tccccctctt tcaagtcagg caggataact
     2761 ttttctattt cattttcttt tagaactttc aagtatccgg gaaattttat acagctacca
     2821 tttgcttcaa aaacatattg ctttgcaccg attctaacat tctttttttc tattaaggcg
     2881 ggtttcattt ggctggcgat aaaatattcc cagatcagct gatagagcct gaattggtca
     2941 ttggttaaat catctttaat ggacaagggt tctaaattaa tagaagtggg tctgattgct
     3001 tcatgggcat cctgtatttt tattaaaggt tccttattct ttgatttttt taatattctt
     3061 ttgtcaaatg tctggtattc tttgccgtat ttctctaaaa tatattttct ggcattaatt
     3121 attgcttcac cagaaagtct tgttgaatct gttctcatat aggtaattaa tcctaatgtc
     3181 cctgtccctg taatagaaat tccttcatat aattgttgtg ccactcgcat agttttcttg
     3241 actgaaaagt ttaatttatg aaatgctatt tgttgcaatg tactcgtttt taaagggagg
     3301 ggtgattttt tatattcttc actaatggtt atttttgaga caatgaatgg gcattcctta
     3361 atatcttcgc aatagaagtc tgcttctttt tggttattta tcttgatttt aatatcattt
     3421 ttggaaatta gagtagcttg aaagacttta tcttcagagt ctttttcctt taacaggaac
     3481 acattgattt tccagtattc ttctgattta aaagcttcaa tttctttctc cctttccact
     3541 attaaatgga cagcaactga ttgtactctg ccagcgctct ttcccctggc gtatttataa
     3601 cataaaggac taactttata acctaccaaa cgatctaaca atcttctggc aatctgagaa
     3661 ttgattatat gcatatccag ctttctagga ttctctatcg cttgtattac tacgttttta
     3721 cttatttcgt taaaagagat tctacatttt tgattaccaa tatccaattc ttctgctaaa
     3781 tgccatgaaa tagcttcacc ttctctatcc gggtcagtag ctaataatat tttgtctttt
     3841 ccttgagcta tattttttat atttttaact atctgccgtt tgctgggaat tataagccag
     3901 gttggtttaa aattattttc tatatcaatt cccattcttg attctggtaa atctttgata
     3961 tgacctttac tggaaatgat cagaaactta tcccctatca tttcttttaa tactctttct
     4021 ttggttggag attcaactat gataagatct tttttcattt ttcatccata tttatttttt
     4081 aaggatatac tcctcttaaa cttggaatat tattttatag ttaaaaatag tcactacaca
     4141 ctatcaagca tcctattttt ttaaataatc tctattagaa attattctat tttcacggtg
     4201 ttgtagcaat cggacaatgt tactgcgatg gcgaaatatt gccaataggt taacaactat
     4261 taaaaaatat ttaaattcga caggtgtctc aaatataaaa gataggataa gcatactaaa
     4321 aacagaaaca attgaaccaa gggctgcaaa tctaaaaact attactaacg ctacccatat
     4381 tattgcagat ataaatgcaa tattgggata aatagatagg atcaccccaa aagttgttgc
     4441 aatgccctta cctcctttga atttcagaaa aatagaccag ttatgaccca taatggcaaa
     4501 aaatccagtt aataattgaa gagtaatact ttcggtagtt attaacttaa caaggtaaac
     4561 tgcaagtagc ccttttaagc agtctccaat aagaaccagc aatgcatatc tgatacccaa
     4621 aacacgaaaa gtgtttgctg ttccaatatt gccgctacca tattccctaa tatcaatctt
     4681 cttcaggtat ttacttacta aataaccaaa aggtatagag cctaaaaaga aactgataat
     4741 cagaagtaag atagttcttg ccaacattat gatttccatc ttatccctgt ctgtctattt
     4801 cctcttttca taagagaata gaataggaat ccctttaaaa tcaaagtttt ttacgaattg
     4861 gttttccata tatctttgat atgaattata tagaagttgg ggattgttta caaatatttt
     4921 aaaggtggga ggtttaattt ttacttgagt gatatagtag aaatggattt ttttgccact
     4981 gactatcttg ggagcccttt tattaacaat attttgtaaa aagttattta attccgaagt
     5041 agatattttc ctgttgtatt ttctaaacat ctcaataatg gtttttaaca caggtaagta
     5101 aacttctgta ggatttaaag ctatggtagt cataataggg gcatctttta aaaattttaa
     5161 actatttttg cttattttta gaagttggtt tttgtcaatt cctatatctt tttttaccag
     5221 atcgaatttg tttaaaagaa cgatacaggc cttcttttcc tcaataatta tgttcgccag
     5281 ccgcttgtct tgcatactga taatatgtgt ggcatccaaa atcaacagga ctatatcggc
     5341 tttcttaata gaatcaattg ttcttaaatt accaaaatat tcaatttttt gttttacatt
     5401 ttttttcctt cttaatccgg cagtatcaat aaatttaatc ttatattcat caaaatatag
     5461 tgaaacttca atggcatctc tggtggttcc agggctttca tccacgat
//
LOCUS       DAOQWT010000030         5445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_15289_length_5445_cov_3.219295, whole genome shotgun sequence.
ACCESSION   DAOQWT010000030 DAOQWT010000000
VERSION     DAOQWT010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15289_length_5445_cov_3.219295"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKJ95_01225"
     CDS             complement(<1..469)
                     /locus_tag="PKJ95_01225"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNR64894.1"
                     /translation="MIQEDRISYLNEKNEREGKYIIYWMQASQRVHYNQALETAIRIA
                     NKRSLPLLVYFEIRTDFPEANLRHYYFMLQGLQGVKHRLNKMGISMVINIQNSPRYPN
                     LSNLAKDAVMVITDCGYLRFQNEWWEKYAVELPCLMIQIESNVVIPVESASNKE"
     gene            complement(710..1759)
                     /gene="ltaE"
                     /locus_tag="PKJ95_01230"
     CDS             complement(710..1759)
                     /gene="ltaE"
                     /locus_tag="PKJ95_01230"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HNR64895.1"
                     /translation="MVINLRSDTQTLPTEEMLEEIRHAPLGDDVFGEDPTVNKLEGMS
                     AERLGKEAALFVTSGTMGNLCALMSHTRPGDEVILEAESHTYFYEVGGFSALAGLSPR
                     LVKGENGIMSVDDIREAMRPEDIHFPPATLLCIENTHNRGGGTVYPVSLMDEIAIFAH
                     EQELKVHIDGARIFNAAVALGVEVKELVKNADSVMFCLSKGLSAPVGSMLVGNKPFIE
                     RARKVRKMLGGGMRQAGVIAAAGIVALEKMVDRLAEDHSNARYIAEELVRVKGLNIDL
                     NTVQTNMVYCDISQLKVSAKQMAELLREKEVLVSAVPPSKIRLVTNRHISREDAKKAI
                     AVIKEVAEKVPSLSN"
     gene            complement(1847..2602)
                     /locus_tag="PKJ95_01235"
     CDS             complement(1847..2602)
                     /locus_tag="PKJ95_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNR64896.1"
                     /translation="MYQRNKFLFEIIIITLLFIILGVAALGQMTAEEIIRRRDDNEYI
                     HSAYAEAVMIINKGGRKVEKSMVMYSRGKDALIEFTNPADRGSKFLKREDNLWMFFPD
                     AEDIVKISGHMLKQGLMGSDFSYQDMMESDKLIDLYSFTLLEEDTLQERPCYVLEAVV
                     IPGEKVSYYKRVIWIDKERFVGLKEELYAESGRLLKVSEVQELKEIDGRWLPVQYIME
                     DKLRKNTFTQFVISKIELNIELDDRIFSLQSLR"
     gene            complement(2632..3885)
                     /locus_tag="PKJ95_01240"
     CDS             complement(2632..3885)
                     /locus_tag="PKJ95_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR64897.1"
                     /translation="MNLLWNLAKKNLFRSRARTIVSVIAIAIAVMAVVFLRGMINGML
                     ESTFKNHIYYKAGHIRVINREYQLKEHLLSLNYPVDGFKGEGYGQMVEKLKELEGVQQ
                     VIPRLKFWAMVTSGEKLINMTGWGVNPEEEIKFTGINRLITEGRMVKSGEREIVMGAG
                     LLDKTGFQVGDRITFLYNTAFDSLKASTFLIVGKIESGLELLNKGLFYIPLEQAQKIL
                     EIDDEVTELLIITSDYKRAGSLLPSIQELFAQESTSGKYLIQLWNRDYDLVEIFVMAK
                     TIYNFIYIFIIILACFVLINTLIMIVNERTREIGMMGALGLKPREILLLFAMEGIIIG
                     VWGSAIGAIVGGVMTKIFSIVGIDFSAALEGVSADLLFESVFYTVFSLENLVFAFILG
                     VVVVTVACMIPARMAARLEPQEALR"
     gene            complement(3897..5144)
                     /locus_tag="PKJ95_01245"
     CDS             complement(3897..5144)
                     /locus_tag="PKJ95_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNR64898.1"
                     /translation="MFSIRLALKNLSRHKRRNLITIFVIGFAFFGYLFAESIMNGMEE
                     MSFDNIKDFETGDVQIANPQYWQEKEDFPLVHLMEWNDDFKDRLAQRTGVEGISPEMR
                     FSALLNNGIDEMGVIGLGVIPQFYNSVFKTRDYVIDGSMLSLEENKAVIGQKLAELMD
                     LKLNDYIVLLVRTKEDTFNTIDVEIGGIIRTLNPILNSTTVFLPLELVQQRLNVGDGI
                     TGISLKIDPGQEKTVLHSLADEFYKKNLPLSVYSWQESAQNVIALGASKKSGIAAIMS
                     VVLLIGMVGIVNNVILSALERTSEIGMMKALGMREWEVVFVFMVEATGIGILGGLLGC
                     LLGFIGVNWLVNNGLNFARIMGEGLDWSEYGVPVLERIYGVWNYPAFSYIFLLGVIVA
                     MLSSIVPSYWAAHKDPVEAIYHR"
     gene            complement(5119..>5445)
                     /locus_tag="PKJ95_01250"
     CDS             complement(5119..>5445)
                     /locus_tag="PKJ95_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR64899.1"
                     /translation="EVGLEGKENRKPGELSGGEKQRVAIARALVKKPKIVLADEPTAN
                     LDSETGQDVIKIMIKMNEELGTTFIFSTHDPQIMERARRFINLKDGMIFSDRREEENV
                     QHTACT"
BASE COUNT         1556 a         1377 c          848 g         1664 t
ORIGIN      
        1 cctctttgtt ggaggcactc tctaccggaa ttaccacatt gctctctatc tgaatcataa
       61 gacagggtag ctccactgca tatttttccc accactcatt ttggaagcga aggtatccac
      121 aatcagtaat aaccatcacc gcatccttag ccagattgct tagattaggg tatctggggc
      181 tattttgaat attgattacc atactgattc ccatcttatt tagcctatgc tttaccccct
      241 gcaaaccctg tagcataaaa taatagtgcc ttaagttggc ttcagggaaa tctgtcctta
      301 tttcaaaata gaccaaaagc ggtaaagacc tcttatttgc tatcctgata gcagtctcca
      361 aagcctgatt ataatgcacc ctctgagacg cctgcatcca gtatattatg tatttgcctt
      421 cccgctcatt tttttcatta agataggaaa ttctatcttc ttgaatcatc tctatctcac
      481 ctcaattcct caatatggaa aatatttgtg tgcttttctc aaaatacctc taccctttac
      541 attcttcaaa tcaatgaaaa ccatataaat gcaaataaaa ggcaatggta acatcaacaa
      601 ggtcattgct agattatgtt cataatactc taattctacc ataaaatatt ggtaataaat
      661 attacattca tttataggca ggcagatagc agagagaaaa cctgaaaaat caattgctta
      721 aagaaggcac tttttctgct acttctttga taaccgctat agccttcttg gcatcttctc
      781 tggaaatatg acgattggta accagacgaa ttttagaggg tggaacagcc gataccagta
      841 cctccttttc tctcaacaat tcagccatct gctttgctga aacttttagc tgactgatat
      901 cacagtaaac catattagtt tgtacagtgt ttaaatcaat gttgagacct tttaccctga
      961 ccaactcttc agcaatatag cgggcattag aatgatcctc tgccagacgg tccaccatct
     1021 tctccaatgc cactatgcct gctgctgcta ttactccagc ctgcctcatc cctccaccta
     1081 acatttttct cacctttctg gctctctcaa taaagggctt attccccacc agcattgaac
     1141 caaccggggc actaagtccc tttgagagac agaacatcac cgagtcagca tttttaacca
     1201 gctctttaac ctctaccccg agtgctacgg cagcattaaa gattcgggct ccatctatat
     1261 gcacttttaa ttcctgttca tgtgcaaata tggctatttc atccattaat gaaaccgggt
     1321 aaacagttcc tcctcctcta ttatgggtat tttcaataca taacagggtt gccggcggaa
     1381 aatgtatatc ttcgggtctc attgcctctc taatgtcatc aacagacata attccattct
     1441 ccccttttac caaacgggga gaaagaccag ccagagctga aaaccctcct acttcataaa
     1501 aataagtgtg ggattctgcc tccagtatta cctcatcacc aggacgggta tggctcatca
     1561 gggcgcataa gttacccatt gttccgctgg ttacaaacaa ggccgcctct ttacccagcc
     1621 tttcagcaga catcccctcc agcttgttaa ctgtaggatc ctccccaaat acatcgtctc
     1681 ccagaggagc atgtcttatc tcctccaaca tttcctcagt aggtaaagtc tgggtatcgc
     1741 tccttaaatt aataaccatt tcttatccac tcctttctct caaatctttc cagagatgtt
     1801 ttaccatctt taacttaatg atacctgaat tatcatcctt ttcttttcaa cgcaaacttt
     1861 gcaagctgaa tatcctatca tccagttcaa tatttaactc aatcttactg ataacaaact
     1921 gggtaaaagt attcttacgc aatttgtctt ccataatgta ctgtaccggt agccagcgac
     1981 catctatctc ttttaattcc tgtacctcac tcaccttgag caatctacca ctttctgcat
     2041 agagctcctc ttttaaaccg acaaaacgtt ctttatcaat ccagataacc ctcttgtaat
     2101 aagaaacttt ctcaccgggt ataaccactg cttccagcac ataacacggt ctctcttgta
     2161 aagtgtcttc ctccaatagg gtaaagctat ataaatcgat aagtttgtct gattccatca
     2221 tatcctgata ggaaaaatca ctgcccatca gaccctgttt cagcatatgg ccggagattt
     2281 taacaatatc ttccgcatca gggaaaaaca tccacagatt gtcttctctt tttagaaact
     2341 tacttccccg gtcagcggga ttggtaaatt ctatcagagc atccttccct ctagaataca
     2401 taaccattga tttttcaact tttctgccac ctttattgat aatcataacc gcctcagcat
     2461 aagcagaatg aatgtattca ttatcatccc gtcgcctgat aatctcttca gcagtcatct
     2521 gtcccagggc ggccactccc agaattataa agagaagagt aattattatt atttcaaaca
     2581 aaaatttatt cctttgatac atttgataaa tacccctttc actatttcta attaacgcaa
     2641 ggcttcctgt ggttctaaac gggctgccat tcgggctggt atcatacagg caactgtaac
     2701 cacaaccacg cccaggataa aagcaaaaac cagattttcc agactgaaga cagtataaaa
     2761 aactgattca aataagaggt cggcagatac gccttccagg gctgcactaa aatcgatgcc
     2821 aactattgag aatatcttag tcataactcc tcccactatg gcccctattg cgcttcccca
     2881 cacaccgata attattccct ccattgcaaa aagtaagaga atttctctcg gttttaaacc
     2941 cagagcaccc atcatcccga tctcccgtgt tcgctcgttt actatcatta tgagtgtatt
     3001 tatcagtaca aaacaggcca ggataataat gaaaatataa ataaaattgt aaatagtctt
     3061 tgccattaca aatatctcca ccaggtcata atccctgttc caaagttgta ttaaatattt
     3121 tcctgaggta ctttcctggg caaacaattc ctggatggag ggcaatagag agcctgccct
     3181 tttataatca gaggtgatta taagcagttc ggtcacctca tcatcaattt ccagtatttt
     3241 ctgtgcctgc tcaaggggta tatagaatag tcctttattt aacaactcca gtccgctctc
     3301 tattttcccc actatcagaa aagtagatgc cttcagggaa tcaaaggctg tattatagag
     3361 gaaggtaatc cggtctccta cctgaaatcc agtcttatct aacaatccgg cacccattac
     3421 tatttctctt tcccctgatt taaccattct tccctcagtg attaatctgt ttataccggt
     3481 aaattttatc tcttcttcag gatttactcc ccagccagtc atattgatta atttctcgcc
     3541 actcgttacc attgcccaaa atttaagccg tggtattacc tgctgaacac cctctaactc
     3601 ttttagcttt tcaaccattt gcccataacc ttcaccttta aaaccatcaa ccgggtaatt
     3661 taaggacaag agatgctctt ttaactggta ctccctatta atcaccctta tatgccctgc
     3721 cttataatag atatggtttt taaaagtaga ttccagcata ccatttatca ttcctcttaa
     3781 gaagaccaca gccataacag caatagctat tgctatgacc gagactattg ttctggccct
     3841 ggagcgaaat agattctttt tagccagatt ccacaacaaa ttcatcttta aactccttat
     3901 cgatggtaga ttgcctctac ggggtcctta tgggcagccc agtaggaggg gacaatactg
     3961 gagagcatcg ctactataac ccccaataaa aagatatagc taaaggcagg ataattccat
     4021 acaccataaa ttcgttctaa taccggtaca ccgtattctg accaatcaag accctctccc
     4081 ataatccggg caaagttcaa tccgttgtta accagccagt ttaccccgat gaaccccagc
     4141 agacatccca gaaggccgcc cagaataccg ataccggttg cctctaccat aaagacaaac
     4201 acaacctccc attccctcat ccccagtgct ttcatcatac ctatctctga agtcctctcc
     4261 agtgctgata gaatcacatt attaacaata ccaaccatac caatgagcag cacaacagac
     4321 ataattgctg cgataccgct ctttttggaa gcacccaatg ctattacatt ctgtgctgat
     4381 tcttgccagg aatagactga taagggtaga ttctttttat aaaattcatc tgctaaagaa
     4441 tggagcaccg ttttctcctg gcccgggtct atcttaagcg agatcccggt aattccatct
     4501 cctacattta acctctgttg aaccagttcc aggggcaaga aaactgtagt gctgttaaga
     4561 ataggattga gagttcgtat aataccccca atttctacat ctatggtgtt gaaggtatct
     4621 tctttggtcc tgaccaacag gacaatataa tcattcaatt tcaaatccat taactctgct
     4681 aatttttgtc ctataactgc cttgttttcc tctaaagaga gcatagaacc atctataaca
     4741 taatcccggg ttttgaatac actattataa aattgtggaa tgacacccag ccctatcaca
     4801 cccatttcgt caattccatt attgagcagt gcagaaaaac gcatttcagg agaaatccct
     4861 tcaactcctg ttctttgtgc cagcctatcc ttaaaatcat cattccattc catcagatga
     4921 accaggggaa aatcttcctt ctcctgccag tattgaggat tggcaatttg gacatcccct
     4981 gtttcaaaat ccttaatatt gtcaaaagac atctcttcca ttccgttcat tatcgattct
     5041 gcaaaaaggt aaccgaaaaa agcaaaacca attacaaaaa tggtaatcag gtttcttctc
     5101 ttgtgtcggg atagattttt aagtgcaagc cgtatgctga acattttctt cctctctcct
     5161 atcggaaaaa atcataccgt cttttaaatt gataaacctt ctcgctcttt ccattatctg
     5221 tgggtcatgt gtggaaaata taaaggtggt tcccagctct tcattcattt taatcattat
     5281 tttaatgaca tcctgaccgg tctctgaatc caggtttgcc gttggctcat cagccagtac
     5341 aattttgggt tttttgacca gcgccctggc aatggctacc ctctgtttct cgccacctga
     5401 tagctcacct ggtttgcggt tctccttgcc ttccagcccc acctc
//
LOCUS       DAOQWT010000031         5366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_15595_length_5366_cov_3.130484, whole genome shotgun sequence.
ACCESSION   DAOQWT010000031 DAOQWT010000000
VERSION     DAOQWT010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15595_length_5366_cov_3.130484"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /gene="tilS"
                     /locus_tag="PKJ95_01255"
     CDS             <1..279
                     /gene="tilS"
                     /locus_tag="PKJ95_01255"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNR64900.1"
                     /translation="NETYLDYEKLKFPLKVRSRKPGDRFQPLNSSYYKKIKSFFIDQK
                     IDRDTRDKTMLVVDNSDRIAWIAGFQTDNRFKVTNKTIKVLHIRQITI"
     gene            419..982
                     /gene="hpt"
                     /locus_tag="PKJ95_01260"
     CDS             419..982
                     /gene="hpt"
                     /locus_tag="PKJ95_01260"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNR64901.1"
                     /translation="MKMIEQEMIEEILIPENQIQKRVEQLGEQISRDYQGKQLFCIGV
                     LRGAIVFLADLARYIKVPMVFDFMSISSYGISTESSGVVRILKDLDENIENKDVLIVE
                     DIIDTGLTLDYLLRMLKSRKPASLKVCTLLNKKERKKIKVPIDYCGFDIPDKFVVGYG
                     LDFNGLYRNIPYILVLKPEYYNKPMDY"
     gene            1157..3004
                     /gene="ftsH"
                     /locus_tag="PKJ95_01265"
     CDS             1157..3004
                     /gene="ftsH"
                     /locus_tag="PKJ95_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNR64902.1"
                     /translation="MAKLNPKKLKNKNFKNAGLYLLILIVAISILSSLFEPASSVKEF
                     SYSQFLNEIEKNNVKSVTISGNTIAGTLNDNQKFSTYLPDDPELMNILRNKNINVEAK
                     PPVELSWWMRILSSLLPMVLIIGIWLFMMRQMQGGGNKVMSFGKSQAKLLGKENPKVT
                     FADVAGVDEAKEELQEVIDFLRNPAKFNQLGAKIPKGILLYGPPGAGKTLLARAVAGE
                     AGVAFFSISGSDFVEMFVGVGASRVRDLFRQAKTNRPCIIFMDEIDAVGRHRGAGLGG
                     GHDEREQTLNQLLVEMDGFDQNTGVILIAATNRPDILDPALLRPGRFDRRIVVDRPDL
                     IGREQILNVHAKGKPLAKEVDLKVLARRTPGFVGSDLANLVNEAALLASRKGKKSIDM
                     EDFEASIDRVIAGPEKKSRIMNEKEKEIVAYHESGHALVAKLLPNCDPVHKVSIIPRG
                     SAALGYTLQLPTEDRYLVSKSELMERLSVLLAGRVAEEITFKDVTTGAQNDLERATKI
                     ARQMVTEFGMSESLGPMTLGRKEQQVFLGRDIAENRNYSEEIAFKIDKEVEKIIENAY
                     NRAKELLVKNKSKLKKIAKNLLEKETLEGDELDILLKGVKLASGQEIMI"
     gene            3339..5198
                     /locus_tag="PKJ95_01270"
     CDS             3339..5198
                     /locus_tag="PKJ95_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR64903.1"
                     /translation="MKNKLIFSIFLIFLLIVGMGISVSAQVPNPERVVYATIGGPETL
                     DPHWCYDTASGEIIFEIYDNLIAYNKESIEDFVPMLALQVPTADNGLIKYGGLDYIFP
                     IRYGVRFHNGEILTPEDIEYSFERAMVFDRAGGPSWMIIEPLLGFTSIEELAVQLAGV
                     ENYSDMFDEDGELLPEYKDVMVKVYTDHIDRAVEVQGNNVVFHLAQPYSPFMKILAHG
                     AGWSAIINKKWSIEQGDWDGKADTWWKYHDPKNEEDPLYNVACGTGPFKLVRWVPEET
                     LMMERFDDHWRGPARLKEVVYQYINEWTTRKLMLQRGDADIIYVPVTNMNEVEDMEGV
                     ELITGLPILSNVVTNMPWELNAEGNANLGSGKLDGEGIPADFFSDIHVRRGFSYLFPY
                     ATFIEKTIHGQGKRNPGPFPEPLSGYTEDEELYYQFDIEKAKEEFKLAWNGELWEKGC
                     KFNIFYNEGNDVRKAVCDMLSTYAKIVNPKFELTSVGVQWSSYLKQFLAEQLPMFTIG
                     WLADYADAYNWAPTYLGSTGTYSGFYGKPYIDFAKENTDPLIEKALKSVDPQEQKDLY
                     EELTRIAHEQAVSLYLYQPIGIHVQRDWVKGWYHNSVISGLPEGADFFYIHKE"
BASE COUNT         1799 a          851 c         1166 g         1550 t
ORIGIN      
        1 aatgaaacct atttggatta tgaaaagctg aaatttcccc ttaaggttag aagccgtaaa
       61 ccgggcgacc gatttcaacc attaaacagt tcttattata aaaagattaa atccttcttt
      121 attgaccaaa agatagatcg tgataccaga gataaaacta tgctggtagt tgataattcc
      181 gatcgcattg cctggattgc tggttttcaa acagataatc gtttcaaagt aactaataaa
      241 accataaaag tattgcatat ccgccaaatt actatttgat tacccattta aactcaatat
      301 atgataaaat tttactatag tatagataag aatagataaa atatgttaac ataatgctat
      361 ttagctgaag tcaataaaag agacactgtt tctggaacag attattaaaa cgaggattat
      421 aaaaatgata gaacaggaaa tgatagaaga gatactaata cctgaaaatc aaattcaaaa
      481 aagagttgaa caattaggtg aacagatttc ccgggattac caggggaagc agctcttttg
      541 tatcggagtg ttaagggggg ctattgtatt tctggctgat ctggcgcgtt acataaaagt
      601 acctatggtt tttgatttta tgtccatttc cagttatgga atatctactg aatcttccgg
      661 ggtagtaagg attttgaagg atctggatga gaatattgag aataaagatg tattgattgt
      721 agaagatata atagataccg gtcttaccct ggattattta ttgagaatgt tgaaatcgcg
      781 aaaaccagcc agcctgaagg tatgtactct gcttaataaa aaggaaagga aaaagattaa
      841 agttcccatt gattattgtg gttttgatat acccgataaa tttgttgttg gctatggttt
      901 agattttaat gggttatacc gtaatattcc ctatatcctg gttctgaaac cagaatacta
      961 taataaacca atggattatt aacaattgga ttaatgaaat ccaatcaaaa ttaaaaatta
     1021 agataattgt gataaataaa tggaaaagaa gacaacgtgc ttgtttgttt ttaatcataa
     1081 aatgtgctaa aattaccttg cgtttaaacc tgaaaaaagt ttaaaacaat ttatataata
     1141 agaggagcaa aaaggattgg caaaattaaa ccctaaaaaa ttaaaaaaca aaaattttaa
     1201 aaatgccggt ttatatctat taattctaat tgtagcaatt tctattctca gctctttatt
     1261 tgagcctgct tcttctgtaa aggagttttc ctattctcaa tttttaaacg aaattgagaa
     1321 gaataatgtt aagagtgtta ctatttcggg aaatactatt gctggtaccc tcaatgataa
     1381 ccagaagttc tcaacatatc tgccggatga cccggaattg atgaacattt tgcgtaacaa
     1441 aaatattaat gtggaagcga aaccaccagt tgagttatcc tggtggatgc gtatactctc
     1501 ttcactttta ccaatggttc ttataattgg tatctggcta tttatgatga gacagatgca
     1561 gggtggcgga aataaggtga tgtcttttgg caagagtcag gcaaagctat tgggtaagga
     1621 aaatcccaaa gttacctttg ctgatgtagc cggtgttgat gaggcgaaag aagaactaca
     1681 ggaagtaata gattttttaa gaaacccggc aaagtttaac cagctgggag cgaagattcc
     1741 caaaggaata cttctttacg gaccaccagg ggctggaaag accttattgg cacgagcagt
     1801 tgccggagaa gctggcgttg ccttttttag catcagtggt tctgattttg tagagatgtt
     1861 tgtgggagta ggggcatcac gagtaaggga tttattcagg caagccaaaa caaacaggcc
     1921 ctgtattatt tttatggatg aaattgacgc tgtgggacgt cacagggggg ctggtttagg
     1981 cggaggacac gatgaaagag agcagacctt gaatcaattg ttagttgaaa tggatggttt
     2041 tgatcaaaat acaggagtta ttctaattgc tgctaccaat agacccgata ttcttgaccc
     2101 ggcactctta agaccgggac gttttgaccg tagaatagta gttgatcgtc cggacctcat
     2161 cggacgtgaa cagatattga atgtccatgc caaagggaag cctctggcta aagaagtgga
     2221 tttaaaggtt ttagccagaa gaacgcccgg ttttgttggt tcagatttag ccaatttggt
     2281 caatgaggca gccttgttag cctcccgcaa gggtaaaaag agtatagata tggaagattt
     2341 tgaggcatcc atcgataggg taattgccgg accggaaaaa aagagcagaa ttatgaatga
     2401 aaaggaaaaa gaaattgttg cttatcatga atccggccat gctctggtgg caaaactgct
     2461 tcccaactgt gaccccgttc acaaggtttc cattataccc agaggaagtg cggctttggg
     2521 ctataccctt caattaccga ctgaagaccg ctatctggtc agtaaatcag aattgatgga
     2581 aagattgagc gttcttttag cgggaagggt tgctgaagag atcactttta aagatgtaac
     2641 taccggggct cagaacgatt tagaaagagc aaccaaaatt gcccggcaga tggtcactga
     2701 atttggtatg agtgaatccc ttggaccaat gactctggga agaaaagaac agcaggtttt
     2761 tttaggacgg gatattgctg aaaatagaaa ctacagcgaa gagattgcct tcaaaattga
     2821 taaagaagta gagaaaatta ttgaaaacgc atacaacaga gccaaggaac tattagttaa
     2881 aaataagagt aagctaaaga aaatagcaaa aaatctgttg gaaaaagaaa ccctggaagg
     2941 agatgaatta gatatcctct taaaaggagt caaacttgcc tccggccaag agattatgat
     3001 ataattcaaa gacggaaaaa tcagagaaga atattagttt tatattgtca actaacgatt
     3061 ttggatatta tacgctccca tttgcaatat tattaaaaaa ataatttatg ctgataagta
     3121 tatctattta aatcataata catgtataat acaaatataa ataaaggctg caacatgttt
     3181 aagatagagc ctgcccaaaa aatacttaga aaaagcagat aattaatttg tggagggagg
     3241 tgaaagtcaa gggtatagtg tgataataaa agaaaaataa tttgataaga aattacttga
     3301 attttctatc aatgtgaaaa ggaaaggagg aaaatagatt gaagaataaa ctaatatttt
     3361 cgattttttt gatattctta ttaatagtgg gaatgggaat atcggttagt gcccaggtgc
     3421 ctaatccgga acgcgtcgtt tatgccacta ttggcggacc agaaaccctt gacccccatt
     3481 ggtgctatga tactgccagt ggtgaaatca tcttcgagat ttacgataac cttattgcct
     3541 ataataaaga gagtattgaa gattttgtac caatgctcgc cttacaggtg cctacagctg
     3601 ataatgggct gatcaaatat ggtggtttag attatatttt cccgattaga tatggtgttc
     3661 gtttccataa cggtgagatt ctgactccgg aggatattga atatagcttt gaaagagcta
     3721 tggtatttga ccgtgctggt ggaccaagct ggatgataat agagccactt ttaggattta
     3781 ctagtattga agaacttgct gttcaattgg ctggtgtgga aaactattca gatatgtttg
     3841 atgaggatgg agagctttta ccggaatata aagatgtgat ggtaaaagta tatacagacc
     3901 atattgacag agcagtagag gtacagggta ataacgttgt gtttcattta gcacaaccct
     3961 attctccttt tatgaaaatt ctagcacacg gtgcaggatg gtctgctatt attaataaaa
     4021 aatggtccat tgagcaggga gattgggatg gtaaagcaga tacctggtgg aaatatcatg
     4081 atcccaaaaa tgaagaagac cccctttata atgtagcctg tgggactggt ccctttaaac
     4141 tggttagatg ggttccggaa gaaaccctga tgatggagag atttgatgac cactggagag
     4201 gaccagccag attgaaggaa gtagtttacc agtatatcaa tgagtggact accaggaagc
     4261 tgatgttaca gagaggggat gccgatataa tctatgtacc ggtaaccaat atgaatgaag
     4321 ttgaggatat ggagggtgta gaattaatta ccggcttgcc aatcttatcc aatgtggtaa
     4381 ccaatatgcc ctgggaattg aatgcagaag gaaatgccaa cctgggaagc ggcaagttag
     4441 atggcgaagg tattccagca gatttcttct ctgatataca tgtccgaaga ggattctcct
     4501 atctcttccc ctatgctact tttattgaaa aaacaataca tggacaaggc aagcgcaatc
     4561 cgggaccttt ccccgagccc ctatctggct atacggaaga tgaggaactc tattaccagt
     4621 ttgatataga aaaggcaaaa gaggaattta agttagcctg gaatggtgag ctctgggaga
     4681 aaggatgtaa attcaatatc ttctataatg aagggaatga tgtccgaaaa gctgtctgtg
     4741 acatgctctc gacttatgcc aagattgtaa atcccaaatt tgagcttact tcggtaggtg
     4801 tacaatggtc ctcctacctg aaacagttcc tggcagaaca actccccatg tttaccattg
     4861 gctggttggc agattatgct gacgcttata actgggcgcc aacctatctg ggtagcactg
     4921 gtacctatag cggattttac ggaaagccct atattgattt tgccaaagag aataccgatc
     4981 ctctaattga aaaagccctt aaatcagttg acccacagga acagaaggat ctctatgaag
     5041 agcttaccag aattgcccat gaacaggctg tttcactata cctctatcaa cctattggta
     5101 tacacgtcca gagagattgg gtcaaaggat ggtaccataa ttcagtaata tccggtttac
     5161 cggaaggtgc tgatttcttc tatattcaca aagaataaat gataagatgt ttgtattttt
     5221 tatcgcgatt ggttaacatt aattaaaaag atggagtgga aatgatacta tactattcca
     5281 ctccattatg ttttcataaa aatattattt tttatgaata ttgattctct ccagtttaaa
     5341 aagatttata atttttttaa aaaaaa
//
LOCUS       DAOQWT010000032         5349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_15654_length_5349_cov_3.150926, whole genome shotgun sequence.
ACCESSION   DAOQWT010000032 DAOQWT010000000
VERSION     DAOQWT010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15654_length_5349_cov_3.150926"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PKJ95_01275"
     CDS             complement(<1..448)
                     /locus_tag="PKJ95_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64904.1"
                     /translation="MLIKRFGKIVNEYKNIIKISNSENRKKPARDYRKLETMKALITV
                     FLIVSIIVVGVNMVFAQCEPETTAGTPVPDKIITVSTFKQLTIEEETFNADIRIPLIS
                     GLENKELENSLNEKYLVEGTKIYEDFKEEIEEIKAQGLEAHLGISSG"
     gene            complement(520..1440)
                     /locus_tag="PKJ95_01280"
     CDS             complement(520..1440)
                     /locus_tag="PKJ95_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019153588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNR64905.1"
                     /translation="MENRLKVSVVQDSPVLFDLEKTMEKVVRITRDIAAGGSKLIVFP
                     ESFIPCYPRGLSFGTVVGNRTEEGREDFQRYFDNSVAVPGPEVDILAELTRELEIYLS
                     IGVTEKNGFGLDGTLHCCNLFFGPKGYLGRHRKLKPTAAERIIWGEDDGSTLTVIDTP
                     YGKMGSLICWENYMPLARMAMYLKGVKLYIVPTADQREIYQSTMKHIAFEGRCFVMMS
                     NQYVEKSMYPKNLKYYKDLENQPDIMCRGGSCIINPYGKYLAGPLFDKPGVLTAELDL
                     NEITRAKFDWDPVGHYNRPDIFKFEVRENR"
     gene            complement(1595..2767)
                     /locus_tag="PKJ95_01285"
     CDS             complement(1595..2767)
                     /locus_tag="PKJ95_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNR64906.1"
                     /translation="MKNLEQEIDKIKDELILMRKDFHRFPEVGFKEYRTAEKVAEYMK
                     NIGLAVQTGIGETGVVGLLRGKKKGTTLGIRACLDALAMEECSGVDYSSENKGVFHGC
                     GHDGNMVMVLGAAKILSRYKGELRGDVKFIFQPSEEETGGAAAFIQAGVLKNPDVEAI
                     VHIHNWHGLKEGVMGVKAGPVMASSDVFKLEIIGKPGHGAWPHLAVDPVVIAADVISA
                     IQNIISREIDPLKPALITIGKIYGGTAVNIIPEKITIEGTVRAYHQEIRNFIAARIEQ
                     IARGITQSARASFNLAYNRIMPPVNNDPEFTAKVSKILKDSFEPDLVTDDIPSEMGCE
                     EFALFQQEIPGLFMFLGNDKEGEPTIPIHDPRYIFNDEILTVGVKALCEIALKWNE"
     gene            complement(3073..3909)
                     /locus_tag="PKJ95_01290"
     CDS             complement(3073..3909)
                     /locus_tag="PKJ95_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002090313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64907.1"
                     /translation="MWFIIGALLLIIGIIIFLCIPSSPTRNDFNSIVERKIKEANGLS
                     EEVFTETDIQILPLPVQHYFRYCGYLGTPKMNYMRASFVDVDFIMSPTRTVKIDYQQL
                     NLVSKPERFALISSSLYGIPFEGLDSYQNGKGSMKGKLAKVITIFDQRGENMDRSSLV
                     TWLSECLLVPNAALNDFIIWKSIDDTNARVTITWEGITVSGIFNFSPEGELLSFRTSD
                     RVAVDMKGEVTKADWSGYFQDYHPVNGILQPKIMKSVWHYLDGDSVYFNQNESPVFIR
                     YQ"
     gene            complement(4071..4805)
                     /locus_tag="PKJ95_01295"
     CDS             complement(4071..4805)
                     /locus_tag="PKJ95_01295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNR64908.1"
                     /translation="MEKLKHNSIFLGIIIVVLLFIVQIFLGKVGHFFAKMISIRQIDP
                     YNIFAEISIHHAIQMLIVLILVLILSKLLKIDFYFKLGNVKKGVKYLILFTATLAVIT
                     VVLHIFMYVNGQLPMYDFPLDRRNILGTLGFQLFLSGPSEEIVFRALPITILVYAFGR
                     SIPIKGYITLEVILASILFSFAHINWSLNPFVFEVDYFRIFYAFVLGIIEGVVFQRSK
                     SILYPMLMHSFSNVLMVGIGYLFLQL"
     gene            complement(5015..>5349)
                     /locus_tag="PKJ95_01300"
     CDS             complement(5015..>5349)
                     /locus_tag="PKJ95_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017472034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64909.1"
                     /translation="TTTREMMIFLKAFFSGRLFNISIFDELSSYNKLQAAMGPICYGS
                     GYMRIPLNSLVTLFMGKGELKGHSGSTGSFAFYYPDKKLFIVGDLNQMANATLPIRLS
                     IRIAMLVK"
BASE COUNT         1693 a         1128 c          747 g         1781 t
ORIGIN      
        1 atccactgct gatacctaag tgagcttcga gcccctgtgc tttaatctct tctatctctt
       61 ctttaaaatc ctcataaatt ttggtaccct caactaagta tttttcattt aaactatttt
      121 ccaattcctt attctccaaa ccggaaataa gaggtatcct tatatctgca ttaaatgttt
      181 cttcctctat ggtgagttgt ttgaaagtag aaacagtgat gattttatct ggtacaggtg
      241 tacctgctgt tgtttctggc tcacattgag caaaaaccat atttacccca actacaatga
      301 ttgatacaat taaaaatact gttatcaaag ctttcatagt ttccaatttt ctgtaatctc
      361 tcgctggttt tttcctattt tcactattgc ttattttaat aatatttttg tattcattaa
      421 ctatctttcc aaacctttta atcaacatct taagcctccc tctattttta gtgtctttta
      481 tattataaca ccattcttta actggcatta ctaattaatt tatctattct ccctgacttc
      541 aaatttaaaa atatcaggtc tgttgtaatg gcccacagga tcccaatcaa attttgctcg
      601 tgttatctca tttaaatcca gctctgccgt taaaacaccc ggtttgtcaa agagaggtcc
      661 tgccagatac ttcccataag gattgatgat acagcttccc cctctacaca taatatcagg
      721 ttgattctcc aaatccttat aatacttcag gtttttggga tacatagact tttctacata
      781 ctgattgctc atcataacaa aacatcttcc ttcaaaggca atatgcttca ttgttgattg
      841 gtaaatttct ctctggtctg cagtgggtac aatatatagt tttacccctt ttaaatacat
      901 agccattctg gcaagtggca tataattctc ccagcagatt aaggagccca tcttcccata
      961 gggagtatct ataacggtaa gggtagagcc atcatcctca ccccagataa ttctttccgc
     1021 cgcagttggt ttaagttttc tgtgtctgcc caaataacct ttcggtccaa aaaacagatt
     1081 acaacaatgg agagtaccat ccagaccaaa cccattcttt tctgtcacac caatagataa
     1141 ataaatctct aactctctag taagttctgc cagtatatcc acctcagggc cgggaacagc
     1201 aacagaatta tcgaaatatc tttgaaaatc ttctctcccc tcttcagttc tgttacctac
     1261 tactgttcca aagctcaatc ctctgggata acagggaata aaactctcag gaaatactat
     1321 tagtttagaa cccccagcgg caatatctct ggtaattcta actacctttt ccattgtctt
     1381 ttccaggtca aacaataccg gcgagtcctg aactaccgat acttttaatc tattttccat
     1441 aacttttacc tttccactct ttcttaccta tttttataac taaaacttat ttcaattttt
     1501 ttaatatact cgatacaagt cattaaaaaa ttataaaaat cataaatcct gtttttttct
     1561 tggaatcttt acaatataac ttgctgttta ttggtcattc attccatttt aaagcaatct
     1621 cacagagtgc ctttacccct acagtaagta tctcatcatt aaaaatatac cgggggtcat
     1681 gtataggtat agtcggctca ccttctttgt cattccctaa aaacataaat aatcctggga
     1741 tctcctgttg gaaaagtgca aattcctcac atcccatttc agatggtata tcatctgtca
     1801 ccaaatccgg ttcaaatgaa tcctttaata ttttgctcac tttagcggta aactccgggt
     1861 cattgttcac cggtggcata attctgttat aggccagatt aaatgatgct cttgctgatt
     1921 gcgtaatacc cctggcaatc tgctctatac gagctgctat aaaattgcga atttcttgat
     1981 gatatgctct cactgtgccc tcaatagtaa ttttttctgg aataatattc accgctgtac
     2041 caccataaat cttaccaatc gtaattaggg caggtttaag gggatcaatc tctctgctaa
     2101 taatattttg aattgcacta attacatctg cagcaatgac caccgggtca actgccagat
     2161 gcggccaggc accatgtccc ggtttgccta ttatttctag tttaaaaaca tcactggaag
     2221 ccatgaccgg acctgcttta actcccatta ccccctcctt caagccatgc cagttgtgaa
     2281 tatggacgat cgcctcaaca tcaggatttt taaggactcc ggcctgaata aaggcagcag
     2341 caccaccggt ttcctcctca gagggctgaa aaataaactt aacatcccct cttaattcac
     2401 ctttatacct tgacagaatc tttgccgctc ccagaaccat aaccatattc ccatcatggc
     2461 cgcagccatg aaataccccc ttattttcag agctatagtc cactcctgaa cattcttcca
     2521 tggcaagggc atccagacag gctcttattc ccagtgttgt tcctttcttt tttcctctca
     2581 agagtcccac tacccccgtc tcaccaatac cagtttgtac tgccaggcct atatttttca
     2641 tatattcagc cactttttct gccgtcctgt attctttgaa acccacctcc gggaaacgat
     2701 ggaaatcttt cctcatcaga atgagttcat ctttgatttt atctatttct tgctccaaat
     2761 ttttcatcac aaatactcct ttaataacaa ttcttatcct actgtcagat tcagcaaccc
     2821 aatacttttt taaaagccca aacttttata aaatataatt aaacaagcaa acataataga
     2881 tatataagct attcttctat attagtatac tatattaaga tttaagtaga aacagaagtt
     2941 ttatagcgat aattctttga aaaaattaaa aagtatatgt tgattttgat aggaagaagg
     3001 aattgaaaac agttgaataa aaggactttt tttccttatt gaccttcttc tttttattta
     3061 aaacatttat aattattgat accgtataaa taccggggat tcattctggt taaaataaac
     3121 gctatctcca tcgaggtaat gccagacaga cttcattatt ttgggctgta aaattccatt
     3181 tactggatgg taatcctgga aatatcctga ccagtctgct ttggttacct cacctttcat
     3241 atctactgca accctgtcgc tggtacgaaa agaaagtaac tctccttctg gagagaaatt
     3301 aaagatacca ctaacagtta taccctccca tgttatagta acccttgcat tcgtatcatc
     3361 gatagacttc caaataataa agtcattcaa tgcagcattt ggtactagta aacattcgga
     3421 aagccaggta actaaagagg aacggtccat gttttcacct cgctggtcaa atatggtaat
     3481 gaccttggcc agtttacctt tcatactgcc tttgccattt tgatatgaat caagtccttc
     3541 aaaaggtatg ccatataaag aggaagagat aagtgcaaag cgttctggct ttgatacaag
     3601 attaagctgt tggtaatcaa tcttaacggt tcttgttggc gacataataa aatctacatc
     3661 tacaaatgaa gccctcatat agttcatttt tggtgttcca aggtacccac agtaacgaaa
     3721 atagtgctgt acaggcaatg gcagtatctg tatatctgtt tctgtaaaaa cctcttctga
     3781 taaaccattg gcttctttga tttttctctc cactatggaa ttgaaatcat tacgggttgg
     3841 agaagaagga atgcataaga atattattat tccaataatt aataacaatg cacctattat
     3901 gaaccacatt tttaaaccct tccttttcat ttttataact ccttcttaca tatttattat
     3961 atttatattc gattttcgag gttcaaaact ttttatgaac acgatatacc gcgatatgca
     4021 caaatcgttt aatcaaatta ttgtttattc aatataatta tttgatagtc ttaaagttgc
     4081 aaaaacaaat agccgatgcc aaccattaac acattgctaa aactgtgcat tagcatagga
     4141 tataaaatgc ttttgcttct ttgaaataca actccttcaa taatcccaag tacaaatgca
     4201 taaaaaatgc gaaaataatc cacctcaaaa acaaacggat tcaaagacca gttaatgtga
     4261 gcgaatgaga ataatattga agccagtatg acttctaatg taatatatcc ttttattgga
     4321 atacttctac caaaggcata tactaatata gttatcggta atgctcgaaa aacaatctct
     4381 tctgaaggtc cacttaaaaa taattggaat cccagggttc cgaggatatt tcttctatct
     4441 aaaggaaaat catacatcgg taactggcca ttaacataca tgaaaatgtg tagaacaact
     4501 gtaataactg ctaaagttgc tgtaaataaa atcaaatatt ttactccttt ttttacgtta
     4561 cctaatttaa aataaaaatc aatttttaac aatttgctta atattagaac taatattaga
     4621 actataagca tttgaatagc atgatgaata gaaatctcag caaaaatatt gtaaggatca
     4681 atttgtcgaa tagaaatcat ctttgcaaaa aagtgcccca cttttcccaa aaatatctgt
     4741 acaatgaaca ataaaacaac aataataatg cccaaaaaaa tgctattatg tttcaacttc
     4801 tccatctaat taaaccctcc ttttttgaca atctatgttt atctaaattt atttctttta
     4861 agacattttt tacaaaaaac actgacaaca gctagaataa gtttatacag atctcaaaac
     4921 ccttatttag acaacatgta gaaaatatag acagataata aaaattaatg actccatcaa
     4981 ctcatcttct ttttaatagc gtattttaac ttttttattt tactaacatt gcgattctta
     5041 tagataacct aatgggtaag gtagcattcg ccatttgatt taaatctcct acaataaata
     5101 gttttttatc agggtaataa aaagcaaatg aacctgtcga accggaatgc ccctttagtt
     5161 caccttttcc cataaagaga gttaccaaac tatttagagg aattcgcata tatccactgc
     5221 catagcaaat cggacccatt gctgcttgca acttattgta agacgaaagc tcatcgaaaa
     5281 tcgaaatatt gaacaatctt ccgctaaaga aagctttaag aaatatcatc atctctcgag
     5341 tagtggtta
//
LOCUS       DAOQWT010000033         5284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_15908_length_5284_cov_3.405814, whole genome shotgun sequence.
ACCESSION   DAOQWT010000033 DAOQWT010000000
VERSION     DAOQWT010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15908_length_5284_cov_3.405814"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PKJ95_01305"
     CDS             complement(<1..318)
                     /locus_tag="PKJ95_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNR64910.1"
                     /translation="MSNRLEIKFGNIIFKNPVVTASGTFSLDYEQHFNLSQLGGICSK
                     GLTRYPRTGNRGIRIWETASGIMNSVGLENKGIDLFNKQDLPLLKKLKEQGVRIIANI
                     NGST"
     gene            complement(311..1018)
                     /locus_tag="PKJ95_01310"
     CDS             complement(311..1018)
                     /locus_tag="PKJ95_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNR64911.1"
                     /translation="MAKIIFNRELDTGIYHIKIAGKYKGAMGQFYMLRGWESHHPVLG
                     RPLSIFDLESESISFIYKVVGEGTHLISRLKESDDIELFGPYGKGFPPPKGRVALIGG
                     GMGIAPLYLAGRELFFDSEIEVVHIYLGFKERAILTDLFEKVSHKLVIVTGGFISQKI
                     NINDYNYVFACGSEEMMKAVVQQGNYGKTQVFVSLEKRMGCGVGACLSCTCQTAKGNQ
                     LTCKDGPVFPGEDIFFE"
     gene            complement(1006..1860)
                     /gene="pyrF"
                     /locus_tag="PKJ95_01315"
     CDS             complement(1006..1860)
                     /gene="pyrF"
                     /locus_tag="PKJ95_01315"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNR64912.1"
                     /translation="MIIDKLYEEALRSPICVGLDFREHLLPEYLLESSASLEEKIVTY
                     NRKIVDATKDIVACYKLQIACYEALGLEGLRAYCRSVQYIRENKKIVIGDVKRGDIEI
                     TAEFYARAHFSGDFEVDFVTMSPYLGEDSISPYYKYLETGEKGIFVLIHTSNKSSGDF
                     QELSIEGQPLYMKVAEKVNQWGESFISSSGLSLIGGVAGLTFPEDMKKIWEACPNAFF
                     LIPGYGIQGGKSIDLTPLLEGKKRFIINSSRDIIGAHKGVKEDHNFAECAREKVQIMK
                     EDILKWQK"
     gene            complement(2096..3208)
                     /locus_tag="PKJ95_01320"
     CDS             complement(2096..3208)
                     /locus_tag="PKJ95_01320"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNR64913.1"
                     /translation="MKLKSMPALKISDLDIHIPIIQGGMAVGISLSGLASAVASAGGV
                     GVIGTAGIGMMEPDFNRNYKGANQRALRKEIRKAKEATDGIIGVNIMVALSDFAEMLN
                     IAVQEEVDLVLMGAGLPLKGMELLIPVYHGEKKRKVVPIVSSARAAKLIFQHWEKCYQ
                     HIPDAVVLEGPLAGGHLGFKREQIDNQDFTLERMLPETISTLQPYADRYGKEIPVIVA
                     GGIYSGKDIFHYLQLGARGVQMATRFVATEECDASLGFKQSYIDCKKDDLVIIDSPVG
                     LPGRAIKNKFLEDVAKGNKKPFKCSWKCLKTCDFKSAPYCIASALTHAQRGDFNSGFA
                     FAGANAYRIEKIITVNELISTLKNEYTHTANKFYSN"
     gene            complement(3221..3664)
                     /gene="fabZ"
                     /locus_tag="PKJ95_01325"
     CDS             complement(3221..3664)
                     /gene="fabZ"
                     /locus_tag="PKJ95_01325"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNR64914.1"
                     /translation="MLNIDDIKKIIPHRYPFLFIDYITELVLGERAVGYKNVTANEPF
                     FQGHFPQEMIMPGVLIIESLAQVGAVAILSQKENKGKLAYFAGINKAAFKRKVVPGDR
                     LKLEVEIIKKKGLFGLGRGIATVDGQIAVKSELLFALEGKNNQIV"
     gene            complement(3686..4921)
                     /gene="fabF"
                     /locus_tag="PKJ95_01330"
     CDS             complement(3686..4921)
                     /gene="fabF"
                     /locus_tag="PKJ95_01330"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNR64915.1"
                     /translation="MKHRIVITGMGVVTPIGNDVATFWANCRKGICGIGFIESFDTSE
                     FKVKIAAEVKDLEKQNVLDYKEARRMDRFTQMAMIASDEALKDAGLNSNEPENNWGVI
                     IGTGIGGFSTVEKENEKILSKGPKKVSPLFIPMAISNIAAANVAIKYGLRGICESVVS
                     ACASGASAIHYGYRLLQQDSATVILCGGSEAAITPLALAGFTSMGALCSHNEPMRASI
                     PFDAKRSGFVMGEGAGIVVMETLENARRRGAKIYAELIGCGATCDAYHITAPDPEGEG
                     GALAMEKAVKEGGIEPSQVSYINAHGTSTPYNDLCETKAIKNVFTDSAYTIPISSTKS
                     MTAHMLGAAGAVEAIVCIKAMEDSFIPPTIGLEEPDPDCDLDYVPKEGRGKELTYTLS
                     NSFGFGGHNVSLLFKKWES"
     gene            complement(4936..>5284)
                     /locus_tag="PKJ95_01335"
     CDS             complement(4936..>5284)
                     /locus_tag="PKJ95_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR64916.1"
                     /translation="RQGRIVNISSVVGIGGNIGQTNYAASKAGIIGFTKSVAKELATR
                     NITVNAVAPGFIKTDMTDKLSEEQKRNILSRIPMVRFGFPEDVANMVHFLVSDEANYI
                     TGQVFRIDGGMEM"
BASE COUNT         1534 a         1287 c          825 g         1638 t
ORIGIN      
        1 agttgaacca tttatattgg caataatcct cactccctgc tctttcaatt ttttcaaaag
       61 aggtaaatcc tgcttattaa ataagtcaat tcccttattc tccagtccta cactgttcat
      121 tattccagag gctgtttccc aaattcttat acctctgttc cctgttcggg gataccgggt
      181 taacccttta ctgcaaattc cgcccaattg ggaaaggtta aaatgttgtt cataatccag
      241 actgaaggtc ccagaagcag tgacaaccgg gtttttaaaa ataatatttc caaatttaat
      301 ctctaatcta ttactcaaag aagatatcct ccccgggaaa taccggccca tccttacagg
      361 ttaactggtt ccccttagca gtctgacagg tgcaggaaag acaggcacct acaccacatc
      421 ccatcctttt ttctaaagag acaaaaacct gtgtttttcc atagtttccc tgttgcacaa
      481 ctgctttcat catctcttcc gaaccacagg caaaaacata attataatca tttatattta
      541 ttttttgaga aataaatcca ccggtaacaa taaccaattt gtgtgacacc ttttcaaaaa
      601 ggtctgttaa aatagctctt tccttaaaac ctaaatagat atggactact tcaatctcag
      661 aatcaaaaaa caattcacga ccagccaaat agagtggagc tattcccatt cctccgccaa
      721 tcagggctac ccttcctttg ggaggaggga atcccttgcc ataaggtcca aagagttcta
      781 tatcatcaga ttcttttaat ctgctaatca gatgagttcc ttctccaact actttataaa
      841 taaaactgat tgattcactc tccagatcaa aaatacttaa gggtctaccc agaacaggat
      901 ggtgagactc ccaccctctt aacatataaa attgccccat agctccctta tattttccag
      961 caattttaat atgatagata ccagtatcca gttcccggtt aaaaattatt tttgccattt
     1021 tagaatatct tctttcatta tctggacctt ttcccgggca cattccgcaa aattatgatc
     1081 ttctttaact cctttatggg caccaatgat atcccgggaa gaattaataa taaatctctt
     1141 tttcccttcc aaaagcggag taaggtcgat tgactttcct ccctgtattc cgtaacctgg
     1201 tattaaaaaa aaggcattgg ggcatgcttc ccaaattttc ttcatatctt cggggaaagt
     1261 tagaccggca actccgccga taaggctgag gccacttgag gaaataaaag attcccccca
     1321 ttgattaacc ttctcagcaa ctttcatgta gagaggctgg ccctctatcg ataattcctg
     1381 aaaatcacca gagcttttat tagaggtatg tataagtacg aagataccct tttcaccagt
     1441 ttccaggtat ttataataag ggctaatact atcttctcct aaatagggac tcatagtaac
     1501 aaaatccact tcgaaatctc ccgaaaaatg agccctggca taaaattccg ctgtaatttc
     1561 aatatcgcct ctttttacat caccaataac tattttctta ttttctctta tatattgcac
     1621 agaacggcaa taggccctca gtccttctag ccccagtgcc tcatagcagg caatctgcaa
     1681 tttataacag gcaacaatat ccttggttgc atcaacaatt ttacgattat aggtaactat
     1741 tttctcttcc aggctagctg aagactcgag caggtattct ggcaataaat gttctctgaa
     1801 atccagtccg acacagattg ggcttctcag tgcctcctca taaagtttat ctataatcat
     1861 ttttaccctc cctcctactt tttaattagt attacatata aaaaaatctt ccccaaaaaa
     1921 tggggaagat tccctgaaaa tcatttttac tttattggtt tttattaggt cttgttttgc
     1981 gataatcaaa agttattacc tttctagtct ctcaggacta aattaaagat actttttcaa
     2041 aaaaatatta ccacctggtt tcagcaatgt caagcaatat tatagcaaat aattcttaat
     2101 tactataaaa tttatttgct gtatgggtat attcattttt taaagtggat ataagttcat
     2161 tgactgttat aattttctca atacggtagg cattggcacc ggcaaaggca aaaccgctat
     2221 taaaatcccc cctctgggca tgagtcagag ctgaggctat acaatagggc gcagacttaa
     2281 aatcacaggt tttcaagcat ttccagctgc atttaaaggg ttttttgttt ccttttgcca
     2341 catcctctaa aaacttattt ttaattgctc ttcccggcaa ccctaccggg ctgtcgataa
     2401 ttaccaggtc atctttttta caatcgatat aggattgctt aaaccccaaa gaggcatcac
     2461 attcttccgt tgcaacgaac cgggtcgcca tttgcacacc tcttgctcct aattgtaaat
     2521 aatgaaaaat atctttacca ctatatattc caccagctac tattaccggt atttcttttc
     2581 catatctatc tgcataaggt tgtaaggtag agattgtttc gggcaacatc ctttccagag
     2641 taaaatcttg attatcaatc tgctctctct taaaaccaag atgccctcct gccagaggcc
     2701 cctctaaaac cactgcatca ggtatatgct ggtaacactt ctcccagtgt tgaaagatta
     2761 gctttgcagc tctggcagag gaaactattg gaacgacttt gcgctttttt tctccatgat
     2821 agacgggaat caatagttcc attcctttta gtggtaaacc ggcacccatt aacaccagat
     2881 ccacctcttc ttgaacagca atatttaaca tttccgcaaa atctgatagg gcaaccatta
     2941 tgtttacccc gataatacca tcggtcgcct ctttagcctt tcttatctcc ttacgcaaag
     3001 cccgctgatt ggcaccttta taattacggt taaaatcggg ctccatcatc ccgattcccg
     3061 cagtaccgat tacccccact ccacctgcac tggcaaccgc tgaagccaac ccagataagg
     3121 agatacctac cgccatacca ccttgaataa taggaatatg gatatctaaa tcactaattt
     3181 ttaaagcagg cattgacttt agtttcatag tcttccttat ctaaactatc tgattatttt
     3241 taccctccag ggcaaacaga agctctgact ttaccgcaat ctgcccatct acggtggcaa
     3301 ttcccctacc cagaccaaaa agtccttttt tctttattat ctcaacttct aatttcaaac
     3361 gatcaccggg aaccactttc ctcttaaaag cggctttatt tatacctgca aaataagcca
     3421 gcttcccctt attctctttt tgacttagaa tagctactgc tcctacttga gctaaagatt
     3481 caatgattaa taccccgggc ataatcattt cctgtggaaa atgtccctgg aaaaaaggtt
     3541 catttgctgt aacgttttta taacctactg ccctttctcc gagtaccagc tcagttatat
     3601 aatcgataaa aaggaagggg taacgatgtg gaataatttt ttttatatca tcaatattta
     3661 acaaatcgcc tctcccttct tctaattaag attcccattt tttaaacaaa agactgacat
     3721 tatgtccccc aaaaccaaaa gaattggata gtgtgtaggt caattctttt ccccgaccct
     3781 ctttaggaac ataatccagg tcacaatccg gatccggttc ttccaatcca atggtaggcg
     3841 gaataaaact atcttccatc gctttaatac agacaatcgc ctccaccgca ccggctgctc
     3901 ctaacatatg ggcagtcatt gatttggtag aactgatggg tatcgtgtag gcactatcgg
     3961 taaaaacatt ctttatggct tttgtttcgc agagatcatt ataaggagtg ctggtaccat
     4021 gtgcattaat atatgatacc tgagaaggtt ctataccacc ttctttaaca gctttttcca
     4081 tagctaacgc tcccccttca ccctccgggt ctggggcagt aatatgataa gcgtcacagg
     4141 ttgccccaca accgataagc tcagcatata tcttagctcc ccttcgcctg gcattctcta
     4201 atgtctccat tactacaatt ccagctcctt cccccataac aaacccgctt ctctttgcat
     4261 caaaaggaat ggaagcccgc atcggttcat tatgagaaca caaagccccc atggaagtaa
     4321 atccggctaa agccagaggg gtgatagctg cctcagaacc tccgcataaa attaccgtgg
     4381 cactatcctg ctgtaataaa cgataaccat aatgtatggc cgatgcaccg gaggcacagg
     4441 cactcacaac actttcgcat atccctcgta atccatattt aatcgccaca tttgctgcag
     4501 ctatattaga aatggccatt ggaataaaaa gtggcgatac tttcttggga cctttgctaa
     4561 ggatcttttc attttccttc tccacagtag aaaagccacc tattccagtt cctataatga
     4621 ccccccaatt attttcaggt tcgttactat tcagcccggc atcttttaaa gcttcatctg
     4681 aagcaatcat ggccatctga gtaaaacggt ccattcttct ggcttcttta taatccagaa
     4741 cattctgttt ctccagatct tttacctctg cggctatctt taccttaaac tcagaagtgt
     4801 caaaagactc aataaaacca ataccgcaaa tacctttccg gcaattagcc cagaaagttg
     4861 caacatcatt tcctattggt gttaccacac ccattccggt aattactatt ctatgtttca
     4921 ttttctgttc tacctttaca tctccattcc accatcaatc cgaaagacct gaccggttat
     4981 ataatttgcc tcatcagata ccagaaaatg taccatattt gccacatctt ccggaaagcc
     5041 aaatcgtacc atgggtatac gggaaagtat atttcttttc tgttcttcag atagtttgtc
     5101 ggtcatatcg gttttaataa acccgggggc aacagcatta acagtgatat tacgggttgc
     5161 taattctttt gccaccgatt tagtaaaacc gataatacca gctttagatg cagcataatt
     5221 tgtctggcca atattaccac caatacccac cactgaagag atattaacga ttctaccctg
     5281 tcgg
//
LOCUS       DAOQWT010000034         5144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_16442_length_5144_cov_3.386323, whole genome shotgun sequence.
ACCESSION   DAOQWT010000034 DAOQWT010000000
VERSION     DAOQWT010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16442_length_5144_cov_3.386323"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1507)
                     /locus_tag="PKJ95_01340"
     CDS             complement(<1..1507)
                     /locus_tag="PKJ95_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HNR64917.1"
                     /translation="MKKSKENRFNCKGINIFLIAIGIIIILTFTVGQSLANTSPPNKQ
                     LINPKDFIIGPAEVDADIPGFSLPLNIADIENYHDFFEKMPLTPKVLELLEKNAFVVV
                     PTPLDIAEQEVFLDSTRELANPKDDFVAYYQVLKDIDVPVFITSDSLLHYYHIFFDTT
                     LMRLERDLFYQDIWEISKELFDVSLNHYNYTEGDLKEAARRNVAYLSVALELLKPDKN
                     QVISNEKLKEEYCFPEMDPEFCEMMIAGVKDVYGEQASYQYFSEEEFGYYDFNVPQFV
                     QDLVQTEINLIKKHQGWEYSPVFIYQEDYSQYVPRGHYTKSEKLKNYFKALMWFGRMT
                     ALIEGSPSLSLGESICSGDIGGIISEYDARIQTLQAFLLAKEYISSRNIQERWRRIYN
                     ITSFIVGFSDDLGPYEYGEVLQKVFSEENGKIDSDRLVEKYDRLKEMIGNYPFNPKIY
                     SGLGACELLMPCPPLTEKGIQDLKMQARRLLKQTKGFRMMGQHFTLDSWLFS"
     gene            complement(1530..3275)
                     /gene="amrB"
                     /locus_tag="PKJ95_01345"
     CDS             complement(1530..3275)
                     /gene="amrB"
                     /locus_tag="PKJ95_01345"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNR64918.1"
                     /translation="MKRLIFIFILFCVGFGTGTMPVVGESMGGLSVAGGVVPHHLLAK
                     EIIDDFFEFIAGQEQHPETIILLSPDHFNCSATKEENSFISVGWDKSVTELEDLSTDI
                     DLLKRLTIDNNIISDRSAVLAEFGITNLLPFIKKYLPETKIVPILIPESITQEEVIKL
                     VQTIDQVSDEQTIIIASVDFSHYLPAGAAIFHDARSIRVLLNFEESEFENIEVDSWQS
                     LYAMRLFAKLRGSENPAIVVHKNSLDFLPCDFNSTTSYFSVVFRKGIPENNLQVETIL
                     LAGDMMLGRGIEELMKENSIYYPFLKIVQLLRDVDVVFANLEGPIVENIEDIEETAMD
                     ESKFNFRPEVLEALKWSRINLLSLANNHIMDRDKEGLEETLDWLQRYQIRFIGTPLSA
                     PVFSHSKNIKNYYFATEQSVFLAFNRVLPFIDYQEEINKGIQKARQSNPEKIIIVSMH
                     WGNEYQAHSGIDERELAGKIVDAGADITIGHHPHVVWEIEMIKGKPVFYSLGNFIFDR
                     QFLPQTREGLVIGLIISTDKLTFHLFPIKNQSGQPILMEQNEKELFLKSLAEKSNKSL
                     RESIKKGIIELELVN"
     gene            3659..4231
                     /locus_tag="PKJ95_01350"
     CDS             3659..4231
                     /locus_tag="PKJ95_01350"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HNR64919.1"
                     /translation="MSKKYIVLIIVLLLLPAFLMTGCSPAPGGGNNGVLPIIVTFNPQ
                     GGEVSPKSKEVTVGSTYGELPVPIKIEYIFLGWFTEKTGGTEVTSSTEVTKTKSHTLY
                     AHWEEFVGPQKTVTVTFDPQYGTVDPASKEVTVGSAYGSLPTPEHPLLFMKFIGWFTA
                     PVGGTEVTSSTTLTIDHDHTLYAHWKNLLL"
     gene            4845..>5144
                     /locus_tag="PKJ95_01355"
     CDS             4845..>5144
                     /locus_tag="PKJ95_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNR64920.1"
                     /translation="MNRQIKKILNIAFITLCLISGITGLTSLAIEQENGIPEGFVYVD
                     ELIPDAILEVRYFSEDNFVGSRVDGYLAPRVILTIEAAETLAEVADELREQGLILK"
BASE COUNT         1639 a         1007 c          892 g         1606 t
ORIGIN      
        1 cagagaataa ccaggaatct aaggtaaagt gctgtcccat cattctaaaa ccttttgtct
       61 gtttaagaag tctcctggct tgcattttta agtcttgaat tcccttttca gtaagtggag
      121 ggcagggcat cagcaattca caggcgccta atccactgta aattttgggg ttaaaaggat
      181 aatttccaat catttccttt agccggtcat atttttcaac caatctatct gaatctattt
      241 tgccattttc ttcagagaat actttttgca gcacttcacc atattcatat ggtcctaagt
      301 catcagaaaa ccctactata aaggaggtaa tattatagat acgtctccat ctttcctgga
      361 tattccggct gctgatatat tcttttgcca gtaaaaaggc ctgaagggtc tgtattcggg
      421 catcatattc ggagatgatt cctcctatat ctccactaca gatagattct ccaagagata
      481 aggagggaga gccttcaatt aaagcagtca ttcttccaaa ccacatcaaa gctttaaaat
      541 aatttttcag cttctcagat ttggtgtaat gtccccgggg aacatattga gaataatctt
      601 cctgataaat aaagacagga gaatattccc agccctggtg ctttttgata agattgattt
      661 cagtctgaac taaatcctgt acaaattgtg ggacattgaa atcataatat ccaaattctt
      721 cctcgctgaa gtattggtag ctggcctgtt ctccataaac atcctttacc ccggctatca
      781 tcatctcaca aaattccggg tccatttccg ggaaacagta ttcctctttt agtttttcgt
      841 tgctgataac ctggtttttg tcgggtttta aaagttctaa agcaacagaa agataggcta
      901 catttcttct ggcagcttct tttaaatcgc cctcagtata attatagtga ttcagggaaa
      961 catcaaataa ttctttactg atttcccaga tatcctgata gaagaggtcc cgctccaggc
     1021 gcattaaagt tgtatcaaaa aagatatgat aataatggag cagggaatct gaggtgataa
     1081 aaacaggcac atctatatct ttcagtacct gataataagc aacaaaatca tccttagggt
     1141 tggctaattc acgggtagaa tccagaaaaa cttcctgctc ggcaatgtcc agaggggtag
     1201 gaacaactac aaaggcattt ttttctaata actctaatac tttcggtgtt aaaggcatct
     1261 tttcaaaaaa atcatggtaa ttttcaatat ctgcaatgtt taaaggtaga gaaaatcccg
     1321 gtatatcagc atcgacttca gccggtccta taataaaatc tttgggattg attagctgtt
     1381 tattaggcgg gcttgtattt gccagcgact gaccaactgt aaaagttaat attattataa
     1441 ttcctatagc aattaaaaat atatttatac ctttacaatt aaatctattt tcttttgatt
     1501 ttttcatttt taaaccccct gatttttctt taatttacca gttctaattc tattatacct
     1561 tttttaatag actctcttaa actcttatta gatttctctg ccaggctttt aagaaataat
     1621 tctttttcat tctgctccat caatattggt tggccggact gattctttat tggaaagagg
     1681 tgaaaggtga gtttatcggt tgaaataatc aaacctatta ccaatccttc tctggtttgc
     1741 ggcaaaaact gccggtcaaa gataaaattt cctaaagaat agaataccgg cttacccttt
     1801 atcatttcaa tctcccagac tacatggggg tgatgcccta tggtaatatc cgctcctgca
     1861 tctacaattt tcccggccaa ttctctttca tcaattccgc tgtgtgcctg atattcatta
     1921 ccccaatgca tgctcacaat gataattttc tctggattgg attgcctggc cttttgaatc
     1981 cccttgttaa tctcttcctg ataatcaata aaaggcaata cccgattaaa ggccagaaaa
     2041 actgattgtt cggtggcaaa gtaataattt tttatatttt tggaatgaga aaagacagga
     2101 gcagaaagag gggttcctat aaaacgtatc tggtaccttt gcagccaatc taatgtttct
     2161 tccaggccct ctttgtctct atccataata tggttattgg ctaaggagag taaattaatc
     2221 cggctccatt ttaatgcttc cagtacttct ggacgaaagt taaatttaga ctcatccatt
     2281 gcagtttcct ctatatcctc tatattttct acaatgggtc cctctaaatt ggcaaaaacc
     2341 acatctacat cccttaagag ctgtacaatt ttcagaaaag gataataaat actattttcc
     2401 ttcattaatt cttctatacc acgcccaagc atcatatctc ctgccagtaa aattgtctca
     2461 acctgtaaat tattttcagg aattcctttc cgaaaaacaa cactaaaata agaggttgta
     2521 gaattaaaat cacagggtaa aaaatcaaga gaatttttgt ggacgacaat agcggggttt
     2581 tcactcccac gcaattttgc aaacaaacgc atcgcatata aagattgcca gctatccacc
     2641 tctatatttt caaattcact ttcttcaaaa ttcaataaca ctctaatact tcttgcatca
     2701 tgaaaaatag cagcaccagc cgggagataa tgggagaaat caacactggc aattattata
     2761 gtctgttcat cggagacctg atcgattgtt tggactaact ttattacttc ttcctgagta
     2821 atgctttccg gaataaggat aggaacaatc ttcgtttcgg gaagatattt tttaataaaa
     2881 ggtaatagat tggtaatgcc aaactctgca agcacagcgc ttctgtctga aattatattg
     2941 ttatcaatgg tcagtctctt taataaatca atatctgtag acaaatcttc tagttcagtg
     3001 acgcttttat cccagccaac actgataaag gaattctctt ctttagtagc cgagcaattg
     3061 aaatgatccg ggcttaaaag aataatagtt tcagggtgtt gttcctgtcc ggcaataaat
     3121 tcaaaaaaat catcaataat ttcttttgcc agcaggtgat ggggcactac acctcccgca
     3181 acagaaagtc ctcccatgct ttccccaaca accggcattg tgccagtacc aaaaccgaca
     3241 cagaacaaga taaaaataaa aattaatctt ttcatgttag gggacggtcc attaacatca
     3301 tttcttttaa taaaaattga aaataatatc tataaactca tatatattct attataaaat
     3361 aaacaaaaga cctaatcatg ttaggggttg cactagctca ataactcggt aacttttccc
     3421 agcttcttat tagggggtaa gatactttat acaaaggcaa tctaataaaa ataccatagc
     3481 ctataaaatc aaagacaaaa cctgtcagat tatagttatt catactcata aaactgtact
     3541 tcttccaaaa ttgttgactt ggggatttat tagtatcata atatgggaaa aatatatata
     3601 agtgaaaaac ctacattttg atatctccaa tataaataaa agaaaggagt atagaaaaat
     3661 gagcaaaaag tatatcgtac taattatagt tttactttta ctaccggcat ttctaatgac
     3721 aggctgtagc cctgctccag gtggaggcaa caatggtgtt ctacccatca ttgtcacctt
     3781 taaccctcag ggtggggaag tcagccctaa aagcaaggag gtgactgtag gttcaaccta
     3841 tggagagcta cctgtgccaa tcaaaatcga gtatatcttc ttaggttggt ttactgagaa
     3901 aacaggcggt accgaagtga ccagctcaac agaagtaacc aaaaccaaaa gtcacaccct
     3961 ctatgcccat tgggaggaat ttgtagggcc acagaaaaca gtcaccgtca cctttgaccc
     4021 ccaatatgga acagtcgacc ctgccagcaa ggaggtgact gttggttcag cctatggatc
     4081 actgcccaca ccagaacatc cgttactgtt tatgaaattt atcggatggt tcactgcacc
     4141 agttggcggt accgaagtga ccagttcaac aacattaacc attgaccatg accataccct
     4201 ctatgcccat tggaagaatc ttttattatg agtgatacag aataaatggt aaaggtcggg
     4261 agaattctat tgagaatccc tcggcctttt ttatgttttg acaactacag agaacaactt
     4321 ttcagtccgg tgagccatta acaggcaaac acctccctga ttatctatag tccacaaagt
     4381 actgcgatat gaaaaccagc atgcaaagac aatttaataa aaacactgtt tgatgattat
     4441 tatctggctg ttcttaatat ttaagtacct cctcatggta ctcatggtag aataaaaaat
     4501 tacaccagaa aagttactta attactgagc aggaacaatg gtcctttgac atctttttaa
     4561 aagctaaaat acactgatat ttggttcagg ttttgagata tagtgaaaaa tgatactcca
     4621 aaatctgatt tcttttttat tatattgtaa tatataaagg aaatattcag agtagcattc
     4681 tggataagtc taccgttgaa ctctcctttt tggaaaactt taacatttat tgaattttgc
     4741 tgaatctttt attatagttg cagaaaagat atacacttaa aaaggataac acttagcttg
     4801 aaacaataca gatgtttctt aaattaaacg aaaggataaa atagatgaac agacagatta
     4861 aaaagattct caatattgcc tttataacat tatgcctcat ttcagggata actggtttaa
     4921 caagcctggc aatagagcag gaaaatggta ttcctgaggg atttgtctat gtcgatgaac
     4981 ttattcctga tgccatactg gaggtaaggt acttctcaga ggataatttt gtgggaagca
     5041 gagtggacgg atacctggct cccagggtca tcttgactat tgaggcggcc gaaaccctgg
     5101 ctgaagttgc tgatgagctt agagagcagg gtttgatttt aaaa
//
LOCUS       DAOQWT010000035         5114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_16578_length_5114_cov_2.938723, whole genome shotgun sequence.
ACCESSION   DAOQWT010000035 DAOQWT010000000
VERSION     DAOQWT010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16578_length_5114_cov_2.938723"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKJ95_01360"
     CDS             <1..508
                     /locus_tag="PKJ95_01360"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNR64921.1"
                     /translation="DEQLKPNLMTCAWGGICCSVPPCINISLRKATYSYNNIVLNKAF
                     TVNILFSDKIKEADYYGLVSGREYDKFAETGITPVKSDIVNAPYGREFPIVLECKLIN
                     SIEIGLHTEFIGEIMDIKVEDNLLDEQGRIDASKLEPILYLPGPKGYYSRGSYLGKGF
                     EIGKSIIS"
     gene            complement(559..1815)
                     /locus_tag="PKJ95_01365"
     CDS             complement(559..1815)
                     /locus_tag="PKJ95_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64922.1"
                     /translation="MSKEKLLNFSKVVLFTLSFLTIISRAALSFESSQKLFYQGSYSL
                     QQQKINNTVTLKIDYMQEITNDLFFQGDLILRTSNKEYSKPFLIGPNEFYLSAYDVLE
                     NLDLKIGQIITRWGAADFFSPLDNFNPAPPELSLTGNQGKIGALGINATYYINSQTYL
                     QAVLLPRLETSPYPDEYLEDSYLTQFKPLYQKQGIDIDAVELSYLPTKNMIWGVQLNH
                     TFPTFDVGLSYYRGYYMDPFPANLDVAFKPTGNIMEVTLSYPARQVLGLEFQGDFPGI
                     EGATLRGDLAYIIPEVWMFQGEKMLEKPYLQTIISADYTTDSNLYLNGGFIYGLPFER
                     GTQCSPYLYLNAKKEITNSDFSPSYTAILSLKDMSMGNAIGIDYQISKNVLATLSYVF
                     ISGDVNSKLGILKPSQGFYFSLEWLF"
     gene            complement(1844..2620)
                     /locus_tag="PKJ95_01370"
     CDS             complement(1844..2620)
                     /locus_tag="PKJ95_01370"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNR64923.1"
                     /translation="MSYIKRFFYIVFLFILILLLNYSASAEQLTVDEIINRVKENQIE
                     YENSRTQAEMVIIDKNGKTEEREIIMYQKEEDDKISILMRFLSPKNVEGVTLLSLDNG
                     DKIQLFMPAYQRTRRISGTTKKDNFMGTDFSYEDLGMDYQNDDYLKKLLEETETQFII
                     EVIPMDEDINYSKFLLYINKEQFYLDKVEFFDLEGTFTKMLEIKEVKIDEEGKNTPTK
                     IEITNIIDNHKTILNIKDIEYDLQLAGDFFSIRTMQKPKL"
     gene            complement(2701..>5114)
                     /locus_tag="PKJ95_01375"
     CDS             complement(2701..>5114)
                     /locus_tag="PKJ95_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR64924.1"
                     /translation="EVEAQELKKNALEDDMLIGNYLSADGNVGLLMVEPKNDIDGSLL
                     RTELEEIINKYKHNVEYYNLFGMPIMDVQITEMALDNMSLAFIAAFVVLSLLFYCFRS
                     IQGTILPIAIALLSSFWILSSVASTGKTVTIVISTIPVLMIALATAYGIHFISRYYEE
                     RQLLSPQEAIKKTIVSVFTPIMMSALTTIAGFVSLSSVVIRPLTEFGIFATIGIFTAF
                     LLTIFLLGAILRVFIPKKVPQNFSYQANDLVTKVLKLVSRSIFVNKKLILGIVAIILV
                     TSSFFISQVKPDSSMETRLGVNNPITISMGYFKEKFGGVDFLYIYLNADNVKHPYILR
                     TIDKIQNYAKHLDTLSQPSSITTFLAQLNSAMENKKIIPSNSDKIDNLWFFTGDNEYI
                     NSMLADEDQSTIIQIRASEMTSSEVNKSTEKVDKFIQSIPNRVREIDLSSLSTEEQKK
                     YLPYLVQDIISSWKANSVKIDEQLEQKLSEKLIQIVRMPVSNFYYSTDKFVTEILQIS
                     ELELEDVGIQSDEMRPILFKYLENSAQNNQFLNLLSEEFALSIDDAKYLKEIIDSSLQ
                     MAGEREKIRYAREEVEHIISSPLTSDDAEYLWYLTDDYVYLPDEQGDIHFSYRLTGTP
                     VIMNAVNESVFHGQIKSMVIAFIIVFILLVVQFGSFLTGLVGMVPIVCTVLTAFGIMG
                     MANISLNIGTMMVASIAIGAGIDYTIHFISRYRQELANNMNEPEKAMKITLTGTGRAI
                     VFNSLAVAAGAFVLTFSEIDMIAEFTRLLSSVMLISVVYTLLLLPLLLHHINSKVLGK
                     SQDKH"
BASE COUNT         1618 a          895 c          907 g         1694 t
ORIGIN      
        1 cgatgaacag ctaaaaccaa acctaatgac ctgtgcctgg ggtggaatat gttgttctgt
       61 tcctccctgt atcaatattt ctttgaggaa ggccacctat tcttataata atatcgtttt
      121 gaataaagcc tttacagtaa atattttgtt tagcgataaa ataaaagagg cagattatta
      181 cggcttagtt tccggtagag aatatgacaa gttcgcagaa acaggcatta ctccggttaa
      241 aagtgatatt gtaaatgccc catatggtag agagtttcct attgttctgg aatgcaaact
      301 tattaattcc attgaaattg gtctacatac cgagtttatc ggtgagatta tggatattaa
      361 agttgaagat aatttacttg atgaacaggg caggatagat gccagtaaac ttgaacctat
      421 tctctatctt ccaggaccga aaggatacta cagtagagga agttatctgg ggaaagggtt
      481 tgaaattggt aagagcatta tttcctgaaa gaaataatgc tctttttaaa tcgccttatt
      541 acgcatcaga taattatttt agaataacca ttccagagaa aaataaaaac cttgagaggg
      601 ttttaatata cccagtttag aattaacatc tcctgatata aagacatagg atagagtggc
      661 gagtacattc ttacttatct gataatctat accaatagca tttcccatac tcatatcctt
      721 taagctcaaa attgcagtat aggatggact gaagtcagaa ttggtaatct cttttttggc
      781 attaagataa aggtatggtg agcactgtgt gcctctttca aagggaagtc catatatgaa
      841 accaccattt aagtaaaggt ttgaatcagt ggtatagtct gcgctaataa ttgtctgaag
      901 ataaggcttt tccagcatct tttctccctg aaacatccat acctcgggta tgatgtaagc
      961 cagatctcct cttaaggtgg caccttcaat gccagggaag tcaccttgaa attccaatcc
     1021 cagtacctgt ctggcaggat agcttaaagt tacctccata atatttcctg taggcttaaa
     1081 agccacatcc agattagcag ggaaggggtc catataatac ccccgatagt aactcagacc
     1141 cacatcaaaa gtgggaaaag tatggtttaa ttgtactccc cagatcatat ttttagttgg
     1201 tagataagaa agttccactg catcaatatc gataccctgt ttctgataga ggggcttaaa
     1261 ctgtgtcaga taggaatcct ccaaatactc atcagggtag ggagaggtct ccagcctggg
     1321 cagtaaaaca gcctgcaaat aagtctggct gtttatatag taggtagcgt taattcccag
     1381 tgcgccaatt tttccttggt taccggttag agataattcg ggaggtgcgg gattaaagtt
     1441 atccaggggg ctgaaaaagt cagccgctcc ccatctggtg ataatttgtc ctatttttag
     1501 gtctaaattt tccagaacat cataggcgct gagataaaac tcattgggtc cgattaggaa
     1561 aggtttggaa tattctttgt tgctggtacg tagtatgagg tctccctgaa agaagaggtc
     1621 attggtgatt tcctgcatat agtctatctt gagggtaaca gtattattaa ttttttgttg
     1681 ctgaagggaa taggagccct gataaaacaa tttttgagat gattcaaatg acagagctgc
     1741 ccgggaaata attgttaaaa atgatagcgt gaataaaacc acctttgaaa aatttaataa
     1801 tttttcttta gacataaaac ttctcctctg aaatttatta tcattatagt tttggttttt
     1861 gcatggttcg gatggagaaa aaatcaccag ccagttgtaa atcgtattca atatccttaa
     1921 tgttcaaaat agttttatga ttatcgataa tattagttat ctcaattttt gttggtgtat
     1981 ttttaccttc ttcatcaatt tttacctctt taatttccag cattttggta aaagttccct
     2041 ctaagtcaaa gaattcgact ttgtcaagat aaaattgttc tttattaata tagagtaaaa
     2101 atttgctata gttaatatcc tcatccatag gaattacttc aattatgaat tgagtctcag
     2161 tttcttctaa gagtttcttt agataatcat cattctgata atccataccc agatcttcgt
     2221 atgaaaaatc agttcccatg aaattatctt tttttgtggt tcccgagatt cttcttgttc
     2281 tctggtatgc aggcatgaaa agctggattt tatcaccatt atctaaactt aacagggtga
     2341 ccccctctac attttttgga gataaaaagc gcattaaaat gcttatttta tcgtcctctt
     2401 ctttttggta cattatgatt tctctttctt ctgtcttgcc atttttatca ataatgacca
     2461 tttctgcctg agttctgcta ttttcatatt caatctgatt ctctttgact ctattgatta
     2521 tctcgtctac cgttaattgc tcagcactgg cactgtagtt taataataaa ataagaataa
     2581 ataaaaatac aatataaaaa aatctcttaa tgtaactcat cttataccct ccttgttttt
     2641 tagtattttc tatttagtta atagcaaaga acatcttaat attatttaat ctttttaaaa
     2701 tcaatgttta tcttgagatt ttcctaaaac cttagaatta atatggtgta acagtaaagg
     2761 caataagagc agtgtgtata ctacgcttat cagcataaca ctactaagca atctggtgaa
     2821 ttcagcaatc atgtcaattt ctgaaaatgt gagcacaaat gcccctgctg ctacagctaa
     2881 agaattaaag acaattgctc gacctgttcc ggttagggtg attttcattg ctttttccgg
     2941 ttcattcata ttattagcta attcttgtct gtatcggcta ataaagtgaa tagtataatc
     3001 aattcctgcc ccaattgcta tgctggcaac catcatagta ccaatattaa gtgaaatatt
     3061 tgccataccc attataccaa aggcggttaa aaccgtacag acgattggaa ccatgcctac
     3121 taatccagtt aaaaaagaac caaactgtac taccaataag ataaaaacta taataaaagc
     3181 tatcaccata ctcttaatct gaccatgaaa aacgctttca tttacagcgt tcataataac
     3241 cggggtaccg gttaatctat aggagaaatg tatatctccc tgttcatcgg ggaggtagac
     3301 atagtcatcg gtgagatacc ataaatattc agcatcgtcg gatgttaaag gtgagctaat
     3361 aatatgctca acttcctctc tggcatatct aattttttct cgctctccag ccatttgcaa
     3421 agaactgtca ataatctctt ttaagtattt agcatcatca atgctaagtg caaattcttc
     3481 tgataataaa tttaaaaatt gattattctg agcagaattt tccagatatt taaagagaat
     3541 aggtctcatt tcatcagatt gaatacccac atcttccagt tctaattctg atatttgtaa
     3601 aatctctgtt acaaacttat ctgtgctata ataaaaattt gaaacaggca ttctgacaat
     3661 ttgaatcaat ttttcactca acttctgttc taattgctcg tcaattttaa cactatttgc
     3721 tttccaggaa gaaataatgt cctgaaccag atagggtaaa tacttttttt gttcttctgt
     3781 tgatagagaa gataaatcta tctctctaac tctattgggg atggactgaa tgaatttatc
     3841 taccttttca gtagatttgt tcacttctga agaggtcatc tcactggctc gaatttgtat
     3901 gatagttgac tggtcttcat ctgcaagcat ggaatttata tattcattgt caccggtaaa
     3961 gaaccataaa ttgtcaattt tgtcagaatt agaaggaata attttcttgt tttccatagc
     4021 actgttcagt tgagctaaaa aagtagtgat agaagagggt tgagaaagag tgtctaaatg
     4081 tttggcatag ttttgtatct tatctatagt cctcaatata taaggatgtt ttacattatc
     4141 tgcatttaag taaatataga gaaaatcaac accaccaaat ttttctttga aataacccat
     4201 agaaatagta attgggttat taactcctaa tctggtttcc atagaagaat cgggttttac
     4261 ctgtgagata aagaaactgg aggtcaccag aataatagca acaatgccca gaataagttt
     4321 tttattaaca aaaattgacc gtgaaactaa ttttagtact ttagtgacca agtcatttgc
     4381 ctgataggag aaattttggg gtactttttt aggtatgaac actcttaaaa ttgcacctaa
     4441 taagaatatg gtcagcagaa aagcggtgaa tataccaata gtggcaaata tgccaaattc
     4501 agttaaaggc cttataacta cggagctaag tgaaacgaaa cctgctatag tggtaagggc
     4561 actcatcatg atgggggtaa aaacagaaac aatagttttt ttaatggctt cctggggaga
     4621 aagaagctgc ctttcttcat aataacgact gatgaagtga ataccgtatg ctgttgccag
     4681 ggcaatcatg agaactggga ttgttgatat gacaatagta actgtttttc ccgttgaagc
     4741 taccgagctt aatatccaga aagaggagag caaggcaatg gcaatgggca aaatagttcc
     4801 ctgtatgctt ctaaaacaat agaaaagaag agatagaacc acaaaggcgg caataaaggc
     4861 taatgacatg ttatcaagag ccatttcggt tatctgaaca tccataatgg gcattccaaa
     4921 caaattataa tactctacat tatgtttgta tttgtttatt atttcttcca gctctgtcct
     4981 taaaagagat ccatcgatat cgttttttgg ctctaccatc agcagtccca catttccatc
     5041 tgctgataga taatttccta ttagcatatc atcttctaag gcgttttttt tgagctcctg
     5101 tgcttctacc tcgg
//
LOCUS       DAOQWT010000036         5101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_16628_length_5101_cov_3.064209, whole genome shotgun sequence.
ACCESSION   DAOQWT010000036 DAOQWT010000000
VERSION     DAOQWT010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16628_length_5101_cov_3.064209"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1624)
                     /locus_tag="PKJ95_01380"
     CDS             complement(212..1624)
                     /locus_tag="PKJ95_01380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNR64925.1"
                     /translation="MKYDFDLICIGLGPAGMAVSAMGAEMGLKVCSIEKNKVGGECMN
                     VGCIPSKSLLRIAKYRHSFIKLQEMGLEEGSMAGVKPPFEKINKYLDYIGEEKNMKMF
                     DKVTLILGEGKASFVDSHTVRVGNRTITGQRIFIAVGSLPMIPPIKGLADIEFMTNQN
                     IFNLSEFPESMTIIGGGAIGCEMAQAFSRLGTRCTIVQMDDFLIPNGEKTTGKLLEEE
                     FKKEGIEVYNSRKITEVKKEDGKVILLTEDGITVTSEKLLVAAGKRFDFGPLKLENAE
                     VKYDKGGIKVDDKYRTTMKHIYAVGDCNGQILLTHAAMHQGMFALMNAVSPWRVFRYR
                     KYVIPWTVFTEPMISFVGMQEDELKEKGIKYHVITAKYENYGAAIAENVSIGYVKVFA
                     SSWGKVFGAIIVGDGSGEMINEWALIIQKKIRLHSILFLAHSFPTMGFLTKRVAEQWM
                     MAKMKSNFLKNIMRFSYRKL"
     gene            complement(2380..2574)
                     /locus_tag="PKJ95_01385"
     CDS             complement(2380..2574)
                     /locus_tag="PKJ95_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006508551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNR64926.1"
                     /translation="MKIFNGETFIIDKKGKKAAAVLPIKKYEELLEDLHDLSIIAERR
                     DEPTISFDELKEKLKKNGDL"
     gene            complement(2869..>5101)
                     /locus_tag="PKJ95_01390"
     CDS             complement(2869..>5101)
                     /locus_tag="PKJ95_01390"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR64927.1"
                     /translation="NTLVIGSEGLNEWLMVEFDTILSFEMPDKGRVIIFSSEGIIYDS
                     LVDSGDISVPEGCLLEVAGEPGAQFQITASVDETAKISKSAAKISEILMADYGVNSLQ
                     YTLLSGNKIIISGSADKHGGGEAVSPDVDTNSTIYGIGSTSKMFTAVAVMLLSEQGKI
                     NLDKPVIDYVPDFVMKDERYKDITVRMLLNHSSGLMGSTYTNGVLFDDNSTFNHDHLL
                     SALANQKLKAEPGAYSVYCNDGFSLAEIVVERVSGLTFSEFVRENITGPLGLENTFTS
                     QDVFDRELMAKTFIRDNETPPEMVNMIGSGGLYSTALELCLFGQIFMNNPENEIAKDF
                     LSPASKDAMNEKEYLKGMWPEQTDNFFNYGLGWDSVELYPFNHYGIKGIAKGGDTQLY
                     HSSLIVLPEQNMSFAVVMSGGSSIFGELMGLSLLQQVLLSEGEIDAIISAHEDISEAV
                     PMPVELTHYAGLYENNTMVTEIKIEQNGTLTMSVVAELTGQNVPEESFIYYSSGKFVS
                     EDKSKKISFVQEANGETYLHMIKNISLPGVGEIIMTSYDSVKISPNQVGLDIQQVWDE
                     RSGVYYLVNEMRSGEMYYNLFQSIFEISTSKELPGYLGTYRIDDKNTASQRAQIPVMN
                     GRDNTSIHYSIDENGKEYLSDGSWIYLSGKGISEIYAGVDAICTIPESGYAQWYFVGK
                     EEEGKTMTVKLPDEGAFAVYDSSECIYYSTVSDNQPVMLPAEGKIVFIGDNPGTKFYI
                     TIK"
BASE COUNT         1530 a         1156 c          713 g         1702 t
ORIGIN      
        1 caataaacct cccacattta tgcccacatc ttttattcca aaaactataa accatgaact
       61 aagaaccact tacacttata cccacatctt tgtatttgca tccccggctt ttcctacatt
      121 ctttatattg tatttttcca aaaaaccaat aactaaaaac catgaacaaa aaactttata
      181 accaacgact actacacaat tccacccaga tttataattt tctataagaa aacctcatta
      241 tattctttaa aaaattactt ttcatttttg ccatcatcca ctgctctgcc accctcttcg
      301 ttaaaaatcc catagtcggg aaagaatggg ccagaaatag tatactatgt aaacgaatct
      361 tcttctgtat tatcaatgcc cattcattta tcatctcacc tgagccatcc cccacaatga
      421 ttgccccaaa aactttaccc cagctgctgg caaatacttt tacatatccg atactcacat
      481 tctccgcaat agcagcacca tagttctcat actttgctgt gataacatga tattttatcc
      541 ctttttcttt taattcatct tcctgcatcc ctacaaaaga aatcatgggt tcagtaaaaa
      601 ccgtccaggg gataacatac tttctatatc taaataccct ccaggggcta actgcattca
      661 taagggcaaa cataccctga tgcattgccg catgggtcag gagaatctgt ccattacaat
      721 cacccacagc ataaatatgc ttcattgtgg tcctatactt gtcatcaact ttaattccac
      781 ccttatcata tttaacttca gcattttcca gcttcagagg accaaaatca aatctcttac
      841 cggcggcaac caataacttc tcagaagtga cagtaattcc atcctcagtc agtaaaatta
      901 ctttcccatc ttcttttttg acttcagtta tttttctgga attataaact tctatacctt
      961 cctttttaaa ctcctcttcc agtaacttcc cggtcgtctt ttctccattg ggtattaaaa
     1021 aatcatccat ctgtacaata gtacatctgg ttcccaatcg actgaatgcc tgggccattt
     1081 cacagccgat agctccccct ccgataatgg tcatgctctc cggaaattcg ctcagattga
     1141 agatattctg attggtcatg aattcaatat ctgccagtcc cttaatgggt ggaatcatcg
     1201 gcaacgaacc aacagcaatg aaaattcttt gacctgttat agtcctgttg cccaccctta
     1261 cagtatgaga atctacaaag cttgcttttc cttcacccaa aataagggta accttgtcaa
     1321 acatcttcat gtttttttct tcaccaatat agtccagata tttattgatt ttctcaaagg
     1381 gtggttttac ccctgccatt gaaccttctt ccaatcccat ttcttgtaat ttaataaaag
     1441 agtgcctgta ttttgcaata cgcagtaatg atttactggg aatgcagcct acattcatac
     1501 attccccgcc aactttattc ttttcaatac tacaaacctt caagcccatc tctgccccca
     1561 ttgcggaaac agccattcct gctggaccca ggccaataca tatcaaatca aaatcgtatt
     1621 tcattttata ctccatttaa cctattttaa taattattta ttatactata aaaaaagtaa
     1681 ggtaacgagc ttttcctctt cactccttct ctttcgtctt aatatttata tttgttatta
     1741 cgaacgctgc gtcagcagcg tgtggcaatc tcatgctcta cagccacact ctttgtattt
     1801 atattttctt tttatttcta aaaaccaaaa acaatgaact ataaatctct ttcaattgtt
     1861 cccacgtctt tattcattaa ataccatcta tcaacgacca acgaccaaat attctcacat
     1921 ttctgtctgc actctttgta ttgtattttt ccaaaaacta aaaaccttta accaaagact
     1981 ttgaacttta tgactaataa tgtaacaaaa acaaaaaagt cgggaagcct ccatggaaac
     2041 ctcccgatcg ttttctattt accttatttg taagtcttta catcaaaacc aaatatgtta
     2101 gaatatgtct attttattta tttaccctat tcttcttcta taaactcctt ttctatgcct
     2161 gatataatag acaataatat ttttttgttc gttatctact atatatacaa ccctgtaact
     2221 accagccctg attcgaaaaa tcttgtttgt accatgaagt ttacgagaat tttggggaaa
     2281 gggatttgtt ttaagaatct caatggcctc aagaatttta gaaatcatgt tttgtcaagg
     2341 gctagtaaat ccttttcagc agactttttc caatatattt tatagatcac catttttctt
     2401 cagcttttct ttaagttcat cgaaagagat agtgggttca tcacgtcttt cagcaattat
     2461 ggaaagatca tgtagatctt caagcagttc ttcatatttt ttaattggaa gtacagcggc
     2521 tgccttctta ccctttttat caataatgaa agtctctcca ttgaatattt tcatttttac
     2581 ctgcctttat ttacatcaat attgggaatt actcttaata ttattctata ttatagttta
     2641 atttattctt attatcaata aacataaaat gtataccttt tgccacttca acatattctg
     2701 caatatctag tttagcattg tattatgtct aaatatacca tcttgtaata taattaattt
     2761 ttatccttat aaatattaat ggaaaataat aaattagtaa gaatattcaa tgatatcaga
     2821 tacaactttg aattatgtca aaggaccgtc ccttgacagg tttgggtttt atttgatggt
     2881 tatgtaaaat ttggttcctg ggttatctcc gataaaaacg atttttccct ctgcaggcaa
     2941 catgacaggc tgattgtccg aaaccgtcga gtagtaaata cattcagagc tgtcatacac
     3001 agcaaaagca ccttcatcag gtaattttac cgtcattgtt ttaccttcct cttctttccc
     3061 tacaaaatac cactgtgcat acccgctttc cggtattgta catatagcat ctacccctgc
     3121 ataaatttca gaaattccct tcccggacag ataaatccag cttccatcag atagatattc
     3181 tttaccattc tcatcaatac tataatgaat tgaagtatta tcccgaccat tcataacagg
     3241 tatctgtgct ctctgactgg cggtattctt atcgtcaatc ctgtatgtgc ccaaataacc
     3301 tggtagctcc ttactggtag atatttcaaa tatcgactga aataaattat agtacatttc
     3361 tccgcttctc atttcgttga ccagataata tactccgcta cgttcatccc agacttgttg
     3421 aatatctaag cccacctgat ttggtgagat tttgacagaa tcatatgagg tcattattat
     3481 ctctcccacc ccgggcagag atatattttt tatcatatgc agataggtct caccattggc
     3541 ttcctgaaca aaagagattt ttttactctt gtcttcactg acaaattttc ctgatgaata
     3601 ataaataaag ctttcctcag gcacgttttg ccctgttaat tcagccacta cggacatagt
     3661 aagtgtgccg ttttgttcta tttttatttc tgttaccatt gtattatttt cataaagtcc
     3721 ggcataatgg gtcagttcta caggcattgg aacagcctca gagatatctt catgggcaga
     3781 aataatagca tcgatttcac cttctgataa aaggacttgt tgcagcaatg aaaggcccat
     3841 caattcacca aaaattgaag agccccctga catcaccaca gcaaaggaca tattctgttc
     3901 aggtaatact attagagaac tgtgatacag ttgagtatct cctcctttgg ctattccctt
     3961 aattccataa tggttaaagg ggtaaagttc tactgaatcc cagcccaatc catagttaaa
     4021 aaaattatct gtttgttcag gccacatccc ttttagatat tctttctcat tcatagcatc
     4081 ttttgaagca ggtgacaaaa aatcctttgc tatctcattt tcgggattat tcataaaaat
     4141 ctgaccaaag aggcagagct ctaaagcggt tgaatataaa cctcctgaac caatcatatt
     4201 taccatttcc ggaggagttt cattatcccg gataaatgtt tttgccatta actcacgatc
     4261 aaaaacatcc tgactggtga aggtattttc caggcctaaa ggcccggtaa tattttctct
     4321 gacaaactca gaaaatgtta aaccacttac cctttccaca acaatctctg caagactgaa
     4381 cccatcatta caataaacag agtaggcacc cggttcagcc ttaagctttt gattggcaag
     4441 agcagaaagc aagtgatcgt gattaaaagt gctattatca tcaaacagga caccgtttgt
     4501 gtatgttgaa cccatcagcc ctgatgaatg gtttaagagc atacgaacgg taatgtcttt
     4561 ataacgttca tctttcatta caaaatcggg tacatagtct ataactggtt tatccaggtt
     4621 tatttttcct tgttcggata gtagcataac tgcaacagca gtaaacattt tactggtaga
     4681 accaatccca taaatagtag aattggtatc cacatcaggc gagacagcct caccaccgcc
     4741 atgtttatct gcagacccgg aaattataat tttattacca gaaagcaggg tatattgaag
     4801 actatttaca ccataatcag ccattaagat ttccgatatt ttagccgccg atttactaat
     4861 ctttgctgtt tcatccactg aagctgttat ctggaactgt gctcctggtt cgccagccac
     4921 ttccagcagg catccttcag gaacagagat atctccactg tccaccagac tgtcatagat
     4981 tataccctca gaactgaata taatgacccg acctttatcc ggcatttcaa aggacaggat
     5041 tgtatcaaat tctaccatca gccattcatt caggccttca ctaccgataa ccagggtatt
     5101 g
//
LOCUS       DAOQWT010000037         5098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_16642_length_5098_cov_3.816181, whole genome shotgun sequence.
ACCESSION   DAOQWT010000037 DAOQWT010000000
VERSION     DAOQWT010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16642_length_5098_cov_3.816181"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PKJ95_01395"
     CDS             <1..228
                     /locus_tag="PKJ95_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64928.1"
                     /translation="NPSGSESFSTANDSVPEGLPKNILETEVKKATKVLGIKENNLVI
                     YKFQVRKLNYVRQEILEEMVKFSKDLNPART"
     gene            complement(191..724)
                     /locus_tag="PKJ95_01400"
     CDS             complement(191..724)
                     /locus_tag="PKJ95_01400"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HNR64929.1"
                     /translation="MKEKIIGILGGMGPEATIDLFQKIVRSTPAKKDQEHIRIIIDNN
                     LKIPDRTKAILYNGENPLPELIKTAQNLERAGADFIIIACNTAHYYLHKIQKTVNIPI
                     FNMMQETALYIHHTFPSLKQVSLFATEGTIHTGLYQAYLDNFAIETLVPTQTEQKELM
                     EIIYGVKSGLDLNLLKI"
     gene            1056..1841
                     /locus_tag="PKJ95_01405"
     CDS             1056..1841
                     /locus_tag="PKJ95_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNR64930.1"
                     /translation="MRIVRYQKNEEVFYGVLQGEEIRTIEGDIFEEFVVRSESVNRRE
                     VNLLSPVNPPNIIAIGLNYKEHADESGQDYPESPLIFLKATSSLIGPEDNIILPDMAP
                     DEVDYEAELVIVIGKRAKNIEMDNVEKYILGYTCGNDVSARDCQLRIDKQWARGKSFD
                     TFCPLGPWIETELANPDNCCIVSRLNGKIMQDSNTSDLIFGVKELVSYCSKNFTLLPG
                     TVIMTGTPSGVGFARRPPVFLKKGDHIEIEIEGIGILSNKVWG"
     gene            complement(1882..3021)
                     /locus_tag="PKJ95_01410"
     CDS             complement(1882..3021)
                     /locus_tag="PKJ95_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HNR64931.1"
                     /translation="MFFQKQYGPIDGFAYILLIFSFASFWFSGYKLVERSNDHLTIAS
                     RFDLGIVMLVLTFIIAGSTDIIFPRAGILISYYFLFSMLAIIIAQQSKSSKIEYGNKP
                     GISNLIFTSIPVILLFASWVLLFFLPQMTSAAQAGYYILKVIANPIGKLLLKILSLLF
                     GSSNLPADVSSTDPAGLSIPIADENELSWLGKILQWIITFGGILLFSVLTILTIGWLL
                     YSLWKWLSLKTELDIERKGFWEELGLWLRHVFLEIKTFLQKYSVSKIFYRRRDDSISV
                     IFKRLCQWGKHSGITRKKFQTPLEYGRCLSLFFPDNIRDIGLIIDGFNKEFYGKKPNG
                     REEIEKIKKAWRRLASPSQWPLRLMTKILYSRKPNLRETFSSHYQ"
     gene            complement(3050..3325)
                     /locus_tag="PKJ95_01415"
     CDS             complement(3050..3325)
                     /locus_tag="PKJ95_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64932.1"
                     /translation="MAKDIKRNRIFLIISSGMMEITWLYALAGVFFIMLNAPVFPIWA
                     ALLSFFTPIIITSSERKGQENYRTPYCAFSFLYFNPALYNIQLRLWE"
     gene            complement(3364..4590)
                     /locus_tag="PKJ95_01420"
     CDS             complement(3364..4590)
                     /locus_tag="PKJ95_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNR64933.1"
                     /translation="MNNPNDVRPLTSVFTDQFFRFLVLILLFVALLLTQKNLILISIL
                     ILTMFYASQLWSHYSVREVYYSFDAEKKKGFPGETISIQAKIYNQKLLPIWIKLFIPM
                     DKSLLSTNNIEDDCLCEEFSLLWYDKFLWQWNLKAQKRGCYQIGPPHLETGDIMGFFQ
                     QKRYLSHSAVEVIIYPRPVSLNFLSTPIKELFGKTGNDHPVKDPVYPIATRDYQHGEP
                     SKFIHWKASARYNHLQSKIFDSSTQRKTLLIIDVGSFQKKNQEGLFERILEVAGAMLL
                     EFDRQGNPYSLVSNGKITGNNMFANLPFATGPEQLSMAMEFLARLTMEEPYPLEKILF
                     NGFDIPAGAGCLYCSYKVNKSIIQISQFLKKHGIPVYFLMAKASYAGRTGSIPLSLLD
                     EIYGEVIDFTEVDSKM"
     gene            complement(4587..>5098)
                     /locus_tag="PKJ95_01425"
     CDS             complement(4587..>5098)
                     /locus_tag="PKJ95_01425"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNR64934.1"
                     /translation="NPIELEGTFPLPEAQLDRFLMCIKLDYPVHDEEMLILERFQKDE
                     PLEKLQPVSTPEEILDLQKERAKVYISQEIKHYIVNLANATRDSSKIRFGASPRASLH
                     LMKSAQALAALRDRDFVLPDDIKELFIPVLAHRIVIETKERLKGTSPQKLLEDILEQT
                     EIPTIGLNK"
BASE COUNT         1648 a          878 c          988 g         1584 t
ORIGIN      
        1 aatccttcag gttctgaatc tttttctaca gcaaatgatt cagtacctga aggattacct
       61 aaaaatatcc ttgaaacgga agtaaaaaaa gccactaaag ttcttggtat aaaagaaaat
      121 aatttagtaa tatataaatt tcaagttaga aaattgaatt atgttagaca ggaaatactt
      181 gaagagatgg ttaaattttc aaaagattta aatccagccc ggacttaacc ccatagataa
      241 tttccatcaa ttctttctgc tctgtttggg taggaaccag tgtctcaata gcaaaattat
      301 cgagataggc ttgatataaa ccggtatgaa tggttccttc tgtggcaaaa agacttacct
      361 gtttcaggga cggaaaagta tggtgaatat ataatgcagt ttcctgcatc atattaaaaa
      421 tggggatatt aaccgttttt tgtattttat gtaaataata gtgagcagta ttgcaggcga
      481 taatgataaa atcagcccct gctctttcca gattttgtgc cgttttaatt aattccggta
      541 agggattttc accattatac agtattgcct tagttcggtc gggaatttta aggttattat
      601 ctataatgat tcgaatatgt tcctggtctt ttttggcggg agtcgatcta acaattttct
      661 ggaataaatc tatagttgcc tcaggcccca ttccacccag aattcctata attttttctt
      721 tcattgtact atcctccaat ttatctaaaa attggcttta tttattactg ataatattat
      781 atattatttt tagagtatat ataactctgt ttaatagaaa tcatcgatca tctcataagt
      841 tattgttaaa aaaatcttag tggagaccac aaaatataaa aaaggggaac atgctgattt
      901 ggtattattc aatccagaga ctatttcaga taaaaatact tatttagaat ccaaaatatt
      961 ccccgaaggt atttatggtg tgtgggtcga cggtgattta aaatataaag cattgtagaa
     1021 aatattttga attaaagaaa aggaaggtta ataagatgag aatagtaagg tatcaaaaaa
     1081 atgaagaggt tttttatggg gtactgcaag gagaagagat taggacaatt gaaggagata
     1141 tatttgaaga atttgtggtt cgttcggaga gcgtaaacag aagagaggta aatttgcttt
     1201 caccggttaa tcctcccaat ataattgcca ttggtttgaa ttataaggaa catgccgatg
     1261 aaagcgggca ggactatcca gaaagcccac tcatcttttt aaaagcaact tccagtttaa
     1321 ttgggccgga agataatatt attcttccgg atatggcacc agatgaggta gattatgaag
     1381 ctgaattagt tatcgtgata ggtaaaaggg caaaaaatat agaaatggat aatgtagaaa
     1441 agtacatact gggatatacc tgtggaaatg atgtatctgc cagagattgc cagctgcgta
     1501 ttgataaaca atgggctaga ggtaaatctt ttgatacttt ttgccctctt ggtccctgga
     1561 tagaaacaga gcttgccaat ccggataatt gctgtatcgt atcacgcttg aatggtaaaa
     1621 ttatgcagga ttccaatacc tcagacctta tctttggggt aaaagaactg gtgagctatt
     1681 gttctaaaaa ttttaccctc ttgccaggta ctgttattat gaccggaact ccatcaggtg
     1741 tcggttttgc tcgcagaccc ccggtatttc taaaaaaggg agaccatatt gagattgaaa
     1801 tagaagggat tggtatattg agtaataaag tatggggata gatcagacac aatctcatcg
     1861 gtttagagaa cattcatttt ttcattgata atgtgaagaa aatgtttccc ttaggtttgg
     1921 ttttctggaa tataatattt tagtcattaa tctcaaaggc cattgagaag gactggcaag
     1981 ccttctccag gcttttttta ttttttctat ctcttctctc ccattgggtt ttttcccata
     2041 gaactccttg ttaaaaccat caatgatcag tcctatatcc cggatattgt ccgggaagaa
     2101 aagagagaga catctgccgt attccaacgg tgtttgaaat ttttttctgg tgataccgct
     2161 atgtttaccc cactggcaga gtcttttaaa tattacagaa atgctatcat ctcttcttct
     2221 gtaaaatatt tttgaaacag agtatttttg tagaaatgtt ttgatttcaa gaaagacatg
     2281 gcggagccag agccctaatt cttcccaaaa accttttctt tcaatatcca attcagtctt
     2341 caaggacaac catttccaga gggaatagag taaccatccg atagttaata tggtcaacac
     2401 actgaataac agaattccac caaaggttat tatccattgc aggattttac ccagccagga
     2461 gagttcattc tcgtcagcta ttggtattga taagcctgcc ggatcggttg agctgacatc
     2521 tgcaggcaaa ttgctggaac caaagagtaa agagaggatt tttagtaata atttaccaat
     2581 aggattggca ataactttaa gaatataata acctgcctga gcggcagagg tcatttgtgg
     2641 taaaaaaaat agcaataccc agctggcaaa aagcagtata accggaatag aagtaaatat
     2701 cagattactt attcctggct tattaccata ctctattttt gagcttttag attgttgggc
     2761 aataataata gccagcatac tgaatagaaa atagtaactg attaaaatac ctgccctggg
     2821 gaagatgata tctgtacttc ccgcaattat aaaggttagg accagcataa caattcccag
     2881 gtcaaatcgg gaggctatgg tgagatgatc attggaacgt tcaactaatt tgtatccgct
     2941 gaaccagaaa gaggcaaagg agaagataag cagtatatag gcaaatccgt caatcgggcc
     3001 atattgtttc tggaaaaaca tttgtaacca tctgagacta aaaaataatt cattcccata
     3061 accgtaattg aatattgtat aaagcaggat tgaaatataa aaaagagaat gcacaataag
     3121 gtgttcgata attctcctgc cccttccttt cagaagaggt aataatgata ggggtaaaaa
     3181 aagacagcaa ggcagcccag ataggaaaga ctggagcgtt taacataata aaaaacactc
     3241 cggccagtgc atagagccag gttatttcca tcataccaga ggagatgatt aagaatattc
     3301 tgtttctttt tatgtctttt gccatctaat tattcccgag cataataatt tattgataga
     3361 tattcacatt ttactatcaa cctcggtgaa atctatcact tcaccatata tctcatctaa
     3421 taaagataaa ggtatagaac cagttcttcc cgcataagag gcttttgcca taagaaaata
     3481 taccggtata ccatgttttt ttaagaattg gcttatttgg ataatacttt tgttgacttt
     3541 ataggaacaa tagagacacc ctgcaccggc aggtatgtca aacccgttaa agagtatttt
     3601 ctccagagga taaggctcct ccattgttaa tctagccaga aattccattg ccatagaaag
     3661 ctgttccgga cctgtggcaa agggaaggtt ggcgaacatg ttatttccag taattttacc
     3721 attagataca aggctataag gatttccctg cctatcaaat tctaaaagca ttgcccctgc
     3781 tacctccagt attctttcaa ataacccctc ctggtttttt ttctgaaatg agccaacatc
     3841 aataattaaa agtgtttttc tttgagtgga agaatcaaaa atttttgatt ggagatgatt
     3901 ataacgggca ctggctttcc aatggataaa ttttgaaggt tctccatgct gatagtcacg
     3961 ggtggcaata ggatagacag gatctttaac cggatggtca ttacctgttt taccgaataa
     4021 ctctttgata ggtgtggaaa ggaaatttaa tgacacaggt ctgggataga tgatcacttc
     4081 aactgctgaa tgggataaat accttttctg ttgaaagaaa cccataatat caccggtctc
     4141 aagatgagga ggtccgatct ggtaacagcc tctcttttgt gccttcaaat tccattgcca
     4201 gagaaattta tcataccaga gcagactgaa ttcttcacaa agacaatcat cttctatatt
     4261 gttagtagag agtaaactct tatccatagg aataaacagt tttatccaga taggcaacaa
     4321 tttttgatta tatatttttg cctgtatgga aatcgtttca ccggggaatc cctttttctt
     4381 ttcagcatcg aaagaatagt agacctctct tacactataa tgactccata attgggaggc
     4441 ataaaacata gttagaatca gaattgagat taagatgagg tttttttgag tgagcaagag
     4501 cgcaacaaac aataatataa gtaccagaaa tcgaaaaaac tggtcagtaa aaacagaggt
     4561 gaggggacga acatcattgg gattattcat ttgttaagtc caattgttgg tatctcggtc
     4621 tgttctaaaa tatcttccaa taatttttgc ggagaagtac ctttcaacct ttctttggtt
     4681 tctattacaa tgcgatgggc cagcaccggg ataaagagtt cttttatgtc atcaggtaaa
     4741 acaaagtctc tgtctcttaa agcagccagt gcctgggctg acttcatcag atgtagagaa
     4801 gccctggggc ttgctccaaa cctgatttta gaggaatctc tggtagcatt tgccagatta
     4861 acaatataat gcttaatctc ctgagaaata tagactttag ccctttcttt ttgtaaatcc
     4921 aggatttctt caggtgtaga aacgggctgc aacttttcta aaggctcatc tttctggaaa
     4981 cgttctaata tcagcatctc ttcatcatga acaggataat ccagcttaat acacattaaa
     5041 aaccggtcca gttgtgcctc ggggagagga aaggttccct ctaattcaat ggggttct
//
LOCUS       DAOQWT010000038         5094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_16665_length_5094_cov_2.967652, whole genome shotgun sequence.
ACCESSION   DAOQWT010000038 DAOQWT010000000
VERSION     DAOQWT010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16665_length_5094_cov_2.967652"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKJ95_01430"
     CDS             <1..283
                     /locus_tag="PKJ95_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HNR64935.1"
                     /translation="ILVAGFLFFLIPNIVRSQSAADSSSGMGFLAAAISTGLATIGAG
                     YAVGAVGSSALGAVSEDPNILGKTLIFVGLAEGIAIYGLIVSIMILGRL"
     gene            344..652
                     /locus_tag="PKJ95_01435"
     CDS             344..652
                     /locus_tag="PKJ95_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HNR64936.1"
                     /translation="MKMFLISDNTHTLAGMRLAGIEGIVVHERREILRELQKVKEMKD
                     LGVLIITELLVERVQPEIDEMKTSKALPIIVEIPDRHGSRRPVDTLSRTIRESIGLKI
                     "
     gene            667..1287
                     /locus_tag="PKJ95_01440"
     CDS             667..1287
                     /locus_tag="PKJ95_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HNR64937.1"
                     /translation="MSDAIEDKISLFTKVILERIELDFQKKQNILTENHKNREKNIIA
                     DYEDKKKQTIEKNVKDAQIRKQHQILKERTRARLVLLKKKNELMNRVFEEVRNKVRTF
                     LPSAEYSDFLREALEKILTRFSPEQFLYFKFSTHDMENRIEMILNTIDSIRGRNTYKV
                     DADKQLIGGLFVKSGDGLLEIDYTVNTILEESYQIIGEVFSTYLNK"
     gene            1414..3225
                     /locus_tag="PKJ95_01445"
     CDS             1414..3225
                     /locus_tag="PKJ95_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNR64938.1"
                     /translation="MPETETGTGAGIGSIIYINGPVVKAKHLETSKVREMVVVGEKKL
                     IGEIISLEGDIGTIQVYEDTIGLKMGEEVIGTGKPLSVKLGPGIVSHIFDGIERPLSK
                     IYDLGNQFIPEGIGLISLDKEELWDTRLTVKEGDFLESGAIFAEIEETSMLLHRAMVP
                     PTLRGRVIRVMPDGKYTIEDTLLILSDEKGQTHNIKMSQEWPIRQPRPVKQRIPLEKL
                     LVTGQRVIDTFFPLAKGGTSGIPGGFGTGKTITQHQLAKWSDADLIVYIGCGERGNEM
                     TEVLEDFPKLKDPKTDNPLMDRTILIANTSNMPVAAREASIYTGITIAEYFRDMGYNV
                     AVMADSTSRWAEALREISGRLEEMPAEEGYPAYLASRLAAFYERAGYCQNLNGSEGSV
                     TIIGAVSPAGGDFSEPVTKSTKRIVGNFLALDRELAHARHFPSINWLLSYSNYIPVLS
                     DWFKENISPEMMGIRAKLMGILLEENKLMDIVKLIGEDVLPDEQRIILEIARIIKIGF
                     LQQSAYHSRDLYTGLPEQYNMLKVIKNLYDRAYSSIKKGIPLSKVKEEGLFSDIIMMK
                     YNISAEKPELFKELIDRINNYYDSLENMYKEGSTDEG"
     gene            3215..>4386
                     /locus_tag="PKJ95_01450"
     CDS             3215..>4386
                     /locus_tag="PKJ95_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNR64939.1"
                     /translation="MKVKYLKLDKAEGPLIVMDKVPEAIYGEMVEIVLPEGENRTGQV
                     VQVDEDRVIIEVFQGSSGISLHDVSVSFSGKPMEIPVSLEVLGRIFNGMAEPIDGAGR
                     IYSQKSFNINGRPMNPIARLYPRNYIQTGISTIDGLMTLIRGQKLPIFSGDGLPHNEL
                     AAQIVKQARLSGEESGDFAIVFTAIGIKHDEADFFLKVFDESGVMDRMTMFLNLAEAP
                     VMERIITPRCALTAAEYLAFECDKHVLVIMTDMTSYCEALREVSSAREEVPSRKGYPG
                     YLYSDLASLYERAGMLKGSKGSITQIEILTMPNDDITHPVPDLTGYITEGQIVLSRTL
                     HQMGINPPINILPSLSRLMKDGIGKEFTREDHSDIANQIFSSYSKVQEIRALAQIIG"
     assembly_gap    4387..4396
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4397..4599
                     /locus_tag="PKJ95_01455"
     CDS             <4397..4599
                     /locus_tag="PKJ95_01455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777634.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNR64940.1"
                     /translation="EDELSNTDQSYMEFGRQFEEKFVHQDFEEYRNMGQTLDIMWELI
                     RILPEQELNRIDPSLLQKYFRK"
     gene            4606..5094
                     /locus_tag="PKJ95_01460"
                     /pseudo
     CDS             4606..5094
                     /locus_tag="PKJ95_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
BASE COUNT         1675 a          866 c         1164 g         1379 t
ORIGIN      
        1 cattctggtt gccggtttcc tctttttctt aattcccaat atagtcaggt cccaatctgc
       61 agcagactct tcttcaggaa tgggattttt agcggcagca atttctaccg gattggccac
      121 cattggtgcc ggttatgcag tgggtgcggt aggttcttcc gcacttggtg ctgtttcaga
      181 agaccccaac attctgggta aaacattaat attcgttggg ctagcggaag gtattgctat
      241 ttatgggttg attgtatcta ttatgattct gggtagattg taatctatat agcaagacaa
      301 aaattatgat tgtgtagaaa agttaaaagg tttgtgaaca gttatgaaaa tgtttttaat
      361 cagtgataat acccataccc tggctggtat gagactggca ggaattgaag gaatagtagt
      421 acatgagagg agagagatat taagggaatt gcagaaagtt aaagaaatga aggatctggg
      481 agtactcatc attaccgaat tgttggtaga aagagtgcag ccagaaatag atgaaatgaa
      541 gacctccaaa gctcttccta ttattgtgga gattccagat cgccatggaa gcaggcggcc
      601 agtagataca ttatccagga ctattagaga atcaatagga ctcaagattt gaggtgaaac
      661 agactaatgt cagatgctat tgaagataaa atttctcttt ttactaaagt tatattggag
      721 agaatagagc ttgattttca aaaaaaacag aatattttaa ccgaaaacca caaaaacaga
      781 gaaaaaaata ttattgctga ttatgaagat aagaaaaaac agaccattga gaagaacgtt
      841 aaagatgccc agattagaaa acagcaccaa attttaaagg aaaggaccag ggcacgactg
      901 gttctattaa agaagaagaa tgaattgatg aatagagttt ttgaagaggt aagaaacaaa
      961 gtcaggactt ttctaccctc agctgaatat agcgattttc taagagaggc cctagagaag
     1021 atattaacca gattctctcc agaacaattc ttatacttta aatttagtac acatgatatg
     1081 gaaaatagaa tcgaaatgat tttaaacacc atagactcta tcagaggcag gaatacctat
     1141 aaggtagatg ctgataagca gctcattggt gggctttttg tgaagagtgg tgatgggctg
     1201 ttagagatag actataccgt aaataccata ttggaggaga gttatcaaat tattggtgaa
     1261 gtgttttcta cctatctgaa taaataactg gacaaagaaa gggacgtaat actgagtaaa
     1321 aatccagatg ttggtagcga aaaagataaa aatagtaata ggaaagaggt agaaacaaag
     1381 ataggatttc agaaaagaat caggagagtt aaaatgccag aaacagaaac gggaacagga
     1441 gcaggaatag gcagtattat ctatataaat ggaccggtag taaaagcaaa acacctggaa
     1501 acttctaagg tgcgtgagat ggtagtggtg ggtgagaaaa aattaatagg tgagattatc
     1561 tccttagagg gcgatatcgg tactattcag gtctatgaag acaccattgg actaaaaatg
     1621 ggggaagaag tcataggtac tggtaaaccc ctatcggtaa agctgggtcc cggaatcgtt
     1681 tcacacatct ttgatggtat agaaagacct ctttctaaaa tatatgattt aggaaatcaa
     1741 tttattccag aagggattgg ccttatctca ctggataaag aagaattgtg ggataccagg
     1801 cttactgtaa aagagggaga tttcttggaa tcaggcgcca tctttgctga aatcgaggag
     1861 acttctatgc tgctccacag ggctatggtt cctcctactc tcaggggaag agtaatcagg
     1921 gtaatgccag atggtaaata taccattgaa gatactctgc ttattctgtc agatgagaaa
     1981 ggccaaacac ataatataaa aatgtcccag gaatggccca tcagacagcc ccgtccggtg
     2041 aaacaaagaa taccactgga gaaattgctg gtaaccggac agagggttat agatactttt
     2101 ttccctttag ctaaaggagg aacttccggt attcccggtg ggtttggcac gggaaagaca
     2161 ataacccaac atcaattagc caaatggagt gatgccgatt taattgttta tattggctgt
     2221 ggtgaacgag gaaatgagat gacagaagtt ttggaagatt tccccaaatt gaaggacccc
     2281 aaaacggata accccttgat ggaccgcact attctgattg ccaatacttc caatatgcct
     2341 gtggcagcca gggaggcttc aatttatacc ggcattacta ttgctgagta cttcagagat
     2401 atgggataca atgtggcagt aatggctgat tctacctccc gctgggcaga agccttgaga
     2461 gagatttcag gcagactgga agagatgcct gccgaagagg gatacccggc atatcttgcc
     2521 tcccgactgg cagcttttta tgagagagcg ggatattgtc aaaatctcaa tggaagcgaa
     2581 ggttctgtaa ctataatagg ggctgtctct ccggcaggag gagatttttc tgaaccggtt
     2641 accaagtcca ccaagaggat tgtcggtaat tttctagctc ttgaccgtga attagcccat
     2701 gccagacatt ttccatctat caactggctc ctatcctaca gtaattatat tcctgttctc
     2761 agtgattggt ttaaagagaa tatatcacca gaaatgatgg gtattagagc caaattgatg
     2821 ggtattttat tagaagagaa taaactgatg gatattgtga aattgatagg tgaagatgtc
     2881 ctgccagatg aacagaggat aatactggaa attgccagaa taataaaaat agggttcctg
     2941 caacagagtg cttaccattc ccgggactta tataccggtc ttccggaaca atataatatg
     3001 ttaaaggtga taaagaacct ttatgacaga gcctattcct ctattaaaaa ggggatacct
     3061 ctatctaaag tgaaagagga aggattgttt tccgatataa tcatgatgaa gtacaatatc
     3121 agtgcagaaa agcctgagct gtttaaagaa ttgatagata ggattaataa ttattatgat
     3181 agtttagaaa atatgtataa agaaggttca actgatgaag gttaagtatc tcaaactgga
     3241 taaagcagaa ggccccctta ttgtaatgga taaggttcct gaggcaatct atggggaaat
     3301 ggtagagata gtattacccg aaggggaaaa tcggaccggt caggtggtac aggtcgatga
     3361 agatagggta attattgaag tttttcaagg ttcttcaggt atatctttgc atgatgtgtc
     3421 ggtaagtttt tcgggaaagc ccatggaaat accagtttca cttgaagtac tgggtaggat
     3481 ttttaatgga atggcagaac ccattgatgg tgccggaaga atctattccc aaaaaagctt
     3541 taatataaat ggcaggccca tgaatcctat agccagactt tatcccagaa actatattca
     3601 gactggaata tcaaccattg atggtcttat gactttgatt cgaggacaaa agctgcccat
     3661 cttttccggt gatggccttc cacacaatga gcttgcagct caaattgtta aacaggcccg
     3721 tctttcagga gaagaatccg gtgattttgc cattgttttt actgctattg gtattaaaca
     3781 tgatgaagct gatttttttc ttaaggtctt tgatgagtct ggtgttatgg acaggatgac
     3841 tatgttttta aatttggcgg aagcccctgt gatggagagg attataactc ccagatgtgc
     3901 cctgaccgct gcagaatatc ttgcctttga atgcgataaa catgtactgg taataatgac
     3961 cgatatgacc agttactgtg aggcattgag agaggtatct tcagcgagag aggaagtacc
     4021 ctcccgtaaa ggttacccgg gatatctcta ttccgattta gccagtctat atgagagagc
     4081 agggatgttg aaaggttcta aagggagtat cacccagatt gaaatattga ccatgcccaa
     4141 tgatgatata acacatccgg tgccagacct taccggctat atcactgaag gccagatagt
     4201 actaagccgc accttgcatc aaatgggcat caatccgccc ataaatattc tgccttcact
     4261 gtcccgcctg atgaaagatg ggattggtaa agaatttacc cgggaagacc attctgatat
     4321 agccaatcag attttttctt cttattccaa agtacaggag ataagggcgc tagcccagat
     4381 aataggnnnn nnnnnngaga agatgagctc tctaataccg atcagagcta tatggagttt
     4441 ggccgtcaat ttgaagaaaa atttgttcat caggattttg aagaatatcg taatatgggg
     4501 caaacactgg atattatgtg ggaacttatc cggattctgc ccgaacagga acttaataga
     4561 atagaccctt ctctgctcca gaaatatttc aggaagtgat gagctatgga agaaaacatt
     4621 gctgctacta aagccaatct tattgctgca caggcagccc tcgatttttc cttgaaaggt
     4681 ttcgatttac tcgataaaaa gagaaatgtc cttatcagaa atataatgga atttgtagaa
     4741 agggcaaaag atgcacagct aaagataaga acattattta tagaggctta cgaagccctt
     4801 actgtagcaa atataaccct gggaataaac taagtaaacg aaattgccca atcaatacca
     4861 caggcaactg aatttaatat tctcaaccgc agtgttatgg gtgtagagat accccaaatc
     4921 aaatatgaac atcacagaat tgaacccagc tatagttttt atcataccaa taccgccttg
     4981 gatgtggctt tacaaaaatt ccaagaggtt aaatatttag tctatgaact ggctgagatt
     5041 gaggactcag tatataaatt ggctcaggag ataaagagaa cccagaaaag aacc
//
LOCUS       DAOQWT010000039         5087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_16704_length_5087_cov_5.287162, whole genome shotgun sequence.
ACCESSION   DAOQWT010000039 DAOQWT010000000
VERSION     DAOQWT010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16704_length_5087_cov_5.287162"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..371)
                     /locus_tag="PKJ95_01465"
     CDS             complement(231..371)
                     /locus_tag="PKJ95_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64941.1"
                     /translation="MQRAEHDKKSFAGGARASYTLTFAAGDIQGDLPETVRAGSQPLA
                     TL"
     gene            complement(1189..4221)
                     /locus_tag="PKJ95_01470"
     CDS             complement(1189..4221)
                     /locus_tag="PKJ95_01470"
                     /inference="COORDINATES: protein motif:HMM:NF017826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HNR64942.1"
                     /translation="MHLLEKIYNNLNHEKIWGPDGLTPLSNFKSNSGGFTACCPNPAH
                     PEKKPSFLMKEGIPAGKCQSCGYHLTWFDAAAIHSGYKDHKVKGGDFWQVFNTLSSLN
                     GGLHLDLPLEEKEYYEHQHEIQQLKNNLPGYLHRQLLESSWAESTRAYLEQRGIPGKY
                     FPRFPMIGFYPSIETIEQYLLSEGFSTEIIQESNVLQKCFQNCCLIFTYRDEIGNTLG
                     FKGRKPSIKLKEIKYQKGFKSEIKDRAIMGLECCNLALKDGERVVCLEGEFDWLTAQV
                     ASLKQHDKTGEFICFGGSDVKPAKLETLKRAGARVLYLSFDPDQAGKKATKEAIKHAE
                     QLDLCPFVVNLPAGLDPDEFIKSQGLEAYKNCVKNSSIAGEWLKQEFFQKYHNLNKEN
                     IEKAKIDLMEEAKHHKGFILDSFLEATAKELKIDHELIRQRMEQRYLELNKGIEAQPL
                     KNYLPDASVPEEFVIPTGWIININGLFKLNHTKEGFKEILIAPAPFYVSSRSENIDTR
                     QEKLEITYLRNNKWKTVYEERSTLMDHRKIVQLSAIGFPVNSINCKEIVNFIHNFEAV
                     NDEKLPKKTTISGFGWKKDKDNIYFVLPGKALGTNISVIFEPEGPGEEGFAKALRTEG
                     DFTTWKRAIRLLISYPKIMFILYASFTAPLLEILDAPNFIIDNWGLSSTGKTTAIEIA
                     ASVWGLPVKELGGLIRGWDATRVFIEREACLLNHLPIFYDDSQTANLKILNNTVYSIA
                     NGVGRQRGAVKGSQKASHWRTIAFSTGEKQLSHISELEGIKARIIDLYGSPFNSTDKA
                     ELIHNVKSLIHNNYGHAGVKFIEELISKGYHKNSETLQKRYNQIYSELTKLVKDNISD
                     RIARYLGAVQLTGELVEKLFDFGADAKEVIQKIFLENCGEIKERGDYPQRALEHIVSW
                     IQGNQNSFDNSEHHATVDKGEIFGIIREEEYIGIFSHKFKEILKRSEFDYSSVLKIFK
                     EKNWIQTSPTTFTYPIGFRGKKIRMIKIVWRALKNLWE"
     gene            complement(4518..>5087)
                     /locus_tag="PKJ95_01475"
     CDS             complement(4518..>5087)
                     /locus_tag="PKJ95_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64943.1"
                     /translation="QVKTGFHSGKQVLTVEKADLERVFNIPCNTGCNSVKQGVNHCAT
                     GVLQVETGVKQSEESLTAERIRQVMEDFFESKQTQLMKPLEEQSIFLAGRLTQENQFL
                     KQRLETVLQEVEQYKALPSKIQEREEEIKTIENRLKQEKEEALKEQEARLKAEIEQAL
                     KQAEEEKKQIVDIWKKELEEAKKPWWKFW"
BASE COUNT         1337 a          928 c          953 g         1869 t
ORIGIN      
        1 tttgattttc aagggcggtt taggggcggg cagaaaatca aatccgggcg ggttggtggc
       61 actaacggtt atcagcaaac ttcagtaaga atctggcaga acggtttaag gggtgggaag
      121 aagatttgat tttcaagggc ggtttagggg cgggcagaaa atcaaatccg ggtgggttgc
      181 cgggaataac ggttatcagc aaaattcatt aaaagcataa cagaacggtt ttaaagggtg
      241 gcaaggggct ggctgccagc tctaacggtt tccggcagat caccctggat atctcctgca
      301 gcaaaagtta acgtatagct ggccctggct ccgcctgcaa aagatttttt atcatgctct
      361 gcacgctgca aagcagagtt tatgctattt tttttctgca gcagctccta tttttgcaat
      421 ggctcggctc tgcggagatg tgccgggaaa acctgcttcc catgggttaa ccggaccggt
      481 gcaagcagtc tataacagcc tcatatcagg gagaagtctc gcgccggttt gccttctgta
      541 cgtagaacgt cttgtgcagc aattctttga gctactgcga aaagatttgc gtcagtctta
      601 gaggtaagat ctggaaagcg agtaacagcg agtcttctcc ctgagtttgg ctggagcgag
      661 atcgccgggt ttatcagctg gagctgttgg gcctgtttgc aatagccggt ataataagat
      721 gttgccgggg tctgatgggt ggggaggggg gagagggccg gaaaaaacat tttaccgatg
      781 ttttatgtat tcatgcggga ttcccaattt catatatatt gtcaaatcgg catgattaca
      841 agcgaaatta cgctagagta aaaaaatgtc aaaatatgga agttactcca agttcgagaa
      901 atttcatgat tttttaaaaa ataaataaat aacaaaaaat aggtgtaacg tgtgtaacct
      961 ggtgtaaaac ctaatatcca taagggtttt taagtgtaac gataggtgta acgataggtg
     1021 taacctagtg taaccttctg aaacgtttgt aacctaagtg taacctatta tgtaacctat
     1081 taaatatata tatagtaagg tttatattaa taaattatat ataggttaca tacgttacac
     1141 taaaaaaatg atttctcatg gagaaaaaat attttttttt agtattaatt attcccacaa
     1201 attttttaag gctctccata cgatttttat cattctaatt tttttacctc taaatccaat
     1261 tggataagta aaagttgttg gagaggtttg tatccaattc ttttctttaa aaatttttaa
     1321 tactgaggaa taatcaaatt cgcttctttt taagatttct ttgaatttat gggaaaaaat
     1381 tcctatatat tcttcttctc gaattatacc aaaaatttct cctttatcca ctgtcgcatg
     1441 atgttcagag ttatcaaaac tgttctgatt tccttgtatc caggaaacta tatgctctaa
     1501 tgctctttgt gggtaatctc ccctttcttt aatctctcca caattttcta aaaatatttt
     1561 ttgtattact tcttttgcgt cagctccaaa atcaaatagc ttttctacca gttcaccagt
     1621 taattgaact gctcctaaat atctggcaat tcgatctgat atattatctt ttactaattt
     1681 ggttaattct gaatatattt gattatatct tttttgtaaa gtttcactgt ttttatgata
     1741 acctttggat attaattctt ctataaattt aaccccagca tgtccgtaat tattatgaat
     1801 taatgattta acattatgta taagctctgc cttatctgtt gagttgaatg gtgaaccgta
     1861 aaggtctatt attcttgctt ttatgccttc taattcggat atatggctta attgtttttc
     1921 gccggttgaa aaagcaatag ttctccaatg ggaagctttt tgagagcctt ttacagctcc
     1981 tctttgtctg cctacaccat tagctatgga ataaactgta ttgtttaaga tttttaaatt
     2041 ggcggtttga gagtcatcat aaaaaattgg taagtgattt aatagacagg cttctctttc
     2101 tatgaatact ctggtagcgt cccatcctct tataagtcct cctaattctt ttactggcag
     2161 tccccagact gaagcagcta tctctatagc agttgttttt cctgtagaac ttaatcccca
     2221 gttatctatt ataaagttgg gagcgtcaag aatttcaagg agtggtgctg taaatgaggc
     2281 atataaaata aacatgattt tggggtaact tatgagaagt cttattgctc ttttccatgt
     2341 ggtaaagtct ccttctgtcc gtaatgcttt tgcaaatccc tcttctcctg gtccttctgg
     2401 ttcaaatatt acagaaatat ttgttcctaa agctttacca ggtaggacaa aatatatatt
     2461 atctttgtct tttttccaac caaagccgga aatagttgtt tttttaggta atttttcgtc
     2521 attaacagct tcaaagttat gaatgaaatt aactatctct ttacagttaa tagagtttac
     2581 agggaaacct attgctgaga gttggactat ttttcgatga tccatgaggg ttgaacgttc
     2641 ttcatagact gttttccatt tattatttct taagtatgtg atttcaagtt tttcttgtct
     2701 ggtatcaata ttttcagagc gagatgatac atagaatggt gctggagcta tgagaatttc
     2761 tttaaatcct tctttggtgt ggtttagctt gaaaagacca tttatattta ttatccaacc
     2821 ggtagggatt acaaattctt ctggaactga tgcatctggc agatagtttt ttaatggttg
     2881 tgcttctatg cctttattta attccagata tctttgttcc atcctctggc ggataagttc
     2941 gtgatctatt tttaattctt tagcagttgc ttcaaggaaa gagtcaagaa taaatccttt
     3001 atgatgttta gcttcttcca taaggtctat tttggctttt tctatatttt ctttattcag
     3061 gttgtgatat ttctggaaga attcttgttt aagccattcg ccggctatag aagaattttt
     3121 gacgcagttt ttatatgctt ctaatccctg agactttatg aattcatctg gatctaatcc
     3181 tgcagggagg ttaactacaa aggggcataa gtcaagttgt tctgcgtgtt taatggcttc
     3241 tttagtggct ttcttgcctg cctggtctgg atcgaaagaa agatagagca ctcttgcccc
     3301 tgctcgttta agtgtttcca gttttgcagg ttttacatcg gacccgccaa aacatataaa
     3361 ttcgccggtc ttatcatgct gtttaaggct tgctacctga gcagtgagcc agtcaaattc
     3421 tccttcaagg cagacaactc tttcaccgtc ttttagtgca aggttgcagc actctaaacc
     3481 catgatggct ctgtctttaa tttcgctttt aaatcctttt tgatatttta tctctttaag
     3541 tttaatagat ggttttcttc ccttaaagcc aagagtattt cctatttcat ccctgtaagt
     3601 aaatatcagg cagcagtttt gaaagcattt ctgtaatacg ttgctttcct ggatgatttc
     3661 tgtgctaaaa ccctctgaaa gcaggtattg ttcaatagtt tctattgatg gatagaatcc
     3721 tatcattgga aatcgcggaa agtatttacc tggtattcct ctttgttcca gatatgctct
     3781 tgtgctttcc gcccatgagc tttccaaaag ttgtctatga agatatcctg gaagattatt
     3841 ttttaattgc tggatctcat gctgatgttc atagtattct ttttcttcca gtggtagatc
     3901 taagtgcagt cctccgttaa gactggataa agtattaaag acctgccaga agtctccgcc
     3961 ttttactttg tggtctttat atcctgagtg gatagcagct gcatcaaacc atgtgaggtg
     4021 atatccgcag gactggcatt tgccggcagg gatgccttct ttcatgagga aagatggttt
     4081 tttctccgga tgtgccgggt tcggacagca ggcagtaaaa ccgccggagt ttgatttaaa
     4141 attgcttaag ggggtgagtc catccggccc ccatattttc tcatgattta gattattata
     4201 tattttttct aataaatgca taactaaact tctccttttt ctctatatat gcaggatttt
     4261 gttataattt atataataat atagtaatgg tattaaatac ctcctacatc ttaaaagtaa
     4321 ggaggtgaga gaaattaaac ctattaattg tttaaagatg ttggtttgct tctgaagccg
     4381 ggtgatcaga ctcggtttct cgttttttaa ccaacttttt tattttatat tccttacccc
     4441 ataataagaa cccatagatt gattctattt ttagtattta taatcctctt tatattgctt
     4501 tatattttaa acatatttta ccagaatttc caccagggtt tttttgcttc ttccaattct
     4561 ttcttccaaa tatctacaat ctgtttcttt tcttcttctg cctgctttaa tgcctgctcg
     4621 atttctgcct tcagtctagc ttcctgttcc ttcagggctt cttctttttc ttgttttaat
     4681 cggttttcaa ttgtttttat ctcttcttct ctttcctgga tctttgatgg taaagcctta
     4741 tattgttcca cttcctgtaa tacagtttct aatctctgct ttagaaattg gttctcctgt
     4801 gttaacctgc cggctaaaaa tatggattgt tcttcaaggg gtttcattaa ctgtgtctgt
     4861 ttactctcaa aaaagtcctc cattacctgc ctgatacgtt ctgctgtcag gctttcttca
     4921 gactgtttta cacctgtttc aacctgtaaa acacctgttg cacagtggtt aacaccctgt
     4981 tttacactgt tacaacctgt gttgcagggt atattgaaaa ccctttctaa atctgctttt
     5041 tctactgtta atacctgttt accactgtga aaacctgttt taacctg
//
LOCUS       DAOQWT010000040         5085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_16713_length_5085_cov_3.095427, whole genome shotgun sequence.
ACCESSION   DAOQWT010000040 DAOQWT010000000
VERSION     DAOQWT010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16713_length_5085_cov_3.095427"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..2407
                     /locus_tag="PKJ95_01480"
     CDS             119..2407
                     /locus_tag="PKJ95_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNR64944.1"
                     /translation="MSRDVKDIIQELEHDYTIRPYLTALEHIPARDAHLDEFPDWLNK
                     SLVKLLQQDGINSLYAHQASALDAVAVGKNVVVVTPTASGKTLCYNLPVLDRIIKEPE
                     SRAIYLFPTKALSQDQLGELYRIVDALDKPISTYTYDGDTPVSARSAIRNQGNIVVTN
                     PDMLHTGILPHHTKWHRLFQNLRYIVLDEIHIYRGVFGSHMANVIRRLKRICQFYGSR
                     PQFICCSATIANPLELAERIVEEKFTLINNNGAPSGEKYFLFYNPPVVNRQLGIRKSL
                     IKETANLVSKFLNHDIQTIVFARSRLTTELLTSYLKDYLDKVGQDRDLVRGYRGGYLP
                     NLRREIEKGLKDGVIKGVVSTNALELGVDIGQLDACFIAGYPGTIASTWQQAGRAGRR
                     THSSVAILVASSVPLDQYIINSSDYFFKETPENAVIDPNNLSILISHIKCAAFELPFV
                     PGEKFGSEELEDILQFLEDKGIVHFTGNKWHWMSEAYPADEVSLRSASTENFAIIDLE
                     EGESKVIGEVDRESAPTTIFEGAIYIHEGEQYTITKLDYDDYKAYAKKVEVNYYTDAQ
                     VESHIKVLDVFDIHEQENIYHQHGEIAITTISTMYKKVKFYTHENLGYGKINLPAEDM
                     HTTAYWLILSETFSEIAGRKMESGFTFDLAGGLLALANVIVNVIPLFVMCDPKDIRSI
                     AQVRSPFTDLPTLYIYDNYPGGVGFSEKIFELRNKILIAARELIENCGCDEGCPSCVG
                     PINQVGNQGKQSALLILKEAIR"
     gene            2410..3714
                     /locus_tag="PKJ95_01485"
     CDS             2410..3714
                     /locus_tag="PKJ95_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNR64945.1"
                     /translation="MDFRERLEKIENLKSKVHQINSTHQKRSIDYSKSSGKATIAEIV
                     PGNVIDTPHGSVFFAEHTIPLDTSYGNTSLLDIQKVFPAGLHIWLPQLERENNDLKLD
                     EILFFDTETTGLAGGSGTYIFLLGLGYFKEESFVVRQYFMSDYHEEEALLWAVNQLFS
                     RDFKLLVSYNGKAYDYPLLQTRYIMMRQPFQLNVSCHLDLLFPARRLWKRRLLDCSLS
                     NIEKRILKIYRNEDIPGYLIPNVYFRYVQNKDARSLKPIFSHNLQDIISLVLLTTKIS
                     QTLDDPLSMEQFALDLCSIGKIYEGYKDFQYSSRCYEEALKGNLSDQEMLDTLKLCSF
                     AYKKQGEWEKAESSWYDIISLSSQFILHPYEELAKYYEHKLKDFHQAVSIVDEALSRL
                     KKENNISWNVKTQWQNSLSKRLDRIKKKQELLKAKNENKNAE"
     gene            3729..4424
                     /gene="radC"
                     /locus_tag="PKJ95_01490"
     CDS             3729..4424
                     /gene="radC"
                     /locus_tag="PKJ95_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNR64946.1"
                     /translation="MEENRKKYTIKDWPENERPRERLLNYGPEYLSDAELLAILINKG
                     YAGKTSVEIARSLLTQFGTLRNIMSASYTEMKKVKGIGKAKYAQIKAALEMGVRLSQE
                     KILPGRKIQKAEDVIDYYSVEMKDKKKELFHMLLLDVRYRIIRDILISMGSLSETTVH
                     PREVIKEVVKESAAAVIFLHNHPSGNPQPSQQDLELTKRLCESCNMLGVKVLDHIIVG
                     DNCFYSFAQMGQI"
     gene            4480..5004
                     /locus_tag="PKJ95_01495"
     CDS             4480..5004
                     /locus_tag="PKJ95_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNR64947.1"
                     /translation="MKQDDLKYCTKCGHLLKSLIIQGKKRIYCHNCDTIYYDNPIPSV
                     AVVTCNEKGQLLLVKRKEEPGKGFWSLPGGFMDDGESAIQAALRELQEETGLQGIVKR
                     FIKIFNQNSDTYGHVIIITYEVGIIGGQLKAGDDAENACFFDIEDLPLLAFSFQKQAI
                     EQVIGCSLPEAGTV"
BASE COUNT         1629 a          857 c         1121 g         1478 t
ORIGIN      
        1 aaagattaaa ttttatatat aattagacga gacctagtgg catatgccac tattaagata
       61 tactatttaa atgaaataaa taaaagaatt tattttagaa caataagaaa gaacccacat
      121 gagtcgtgat gttaaagata taattcagga attagagcac gattatacca tccgccctta
      181 tttaacggcc ttagagcata taccggcaag ggatgcgcat ttagatgaat ttccggattg
      241 gcttaacaaa agtttggtga aactgctaca acaggatggt ataaacagtc tctatgcaca
      301 ccaggcaagt gcacttgatg ctgttgctgt agggaaaaat gtggtggtag taaccccgac
      361 tgcttctggt aaaacattat gttacaatct gcctgttcta gacagaatta ttaaggaacc
      421 ggaaagccgg gcaatatatc ttttcccgac taaagctctt tcccaggacc agctgggaga
      481 gctttatcgt atagtagatg ctttagataa acctatcagc acttatacct atgatgggga
      541 tacgccagtt tcagctcgtt ctgctattcg taatcagggt aatattgtcg tgacaaaccc
      601 ggatatgctg cataccggta tactgccaca ccataccaaa tggcatcgtt tgtttcagaa
      661 tctaagatac atagtactcg atgaaatcca tatttaccgt ggtgtatttg gcagtcatat
      721 ggctaacgta atacgtcggt taaagcggat ttgtcagttt tatggctcca ggccacagtt
      781 tatctgctgt tcggcaacaa ttgccaatcc cctggaatta gctgaaagga ttgtggagga
      841 aaaatttacc ctgataaata ataatggtgc acccagcggg gaaaagtatt ttctattcta
      901 caacccgccg gtggtaaacc gacagctggg tatcaggaag agcctcatca aagaaacggc
      961 caatctggta agcaagtttt taaatcatga tattcagact atcgtttttg cccgcagtcg
     1021 cctgactaca gagcttttaa ccagttatct gaaggattat ctggataaag ttggccagga
     1081 ccgcgatctg gttagaggtt atcgaggggg ttatctaccc aatttacgtc gggaaataga
     1141 aaaaggactt aaagatggag taatcaaagg agtggtcagc actaatgcct tagaattagg
     1201 agtcgatatt ggtcagctgg atgcctgctt tattgccggt tatccgggta ctattgccag
     1261 tacctggcaa caggcgggac gagccggtag gcgtacccat tcttctgtgg caatattagt
     1321 tgcctccagt gtacctctgg atcaatatat tatcaattcc tctgattact tttttaaaga
     1381 gactccggag aatgctgtaa ttgaccctaa taatctgagt atactgatta gccatataaa
     1441 gtgtgctgct tttgaattgc cctttgtccc tggagagaaa tttggctcag aagaactgga
     1501 agatatactg caatttttgg aggataaagg gatagttcat tttaccggaa acaaatggca
     1561 ctggatgagt gaggcatatc ctgctgatga ggtcagtctg cgcagcgcct ccaccgaaaa
     1621 ctttgctatt attgatttag aggaaggaga gagcaaagtt attggagagg tagaccggga
     1681 aagtgctccc actaccattt ttgaaggcgc catttatatc cacgaaggag aacaatatac
     1741 tattaccaaa ctcgattatg acgattataa agcctatgcc aaaaaggtag aggttaatta
     1801 ttataccgat gcacaggtag aaagtcatat aaaggttctg gatgtctttg atatacatga
     1861 acaggaaaat atttaccatc agcatggaga aatagctatt accactattt ctactatgta
     1921 taagaaggta aaattctata ctcatgagaa tttgggatat ggaaaaataa atttaccggc
     1981 agaagatatg catactactg cctattggtt aattttgtcg gaaacttttt ccgaaatagc
     2041 ggggaggaag atggagtctg gatttacctt tgatctggca ggtggattac tggctctggc
     2101 taatgttata gttaatgtga taccattatt tgtaatgtgt gaccccaaag atatcaggtc
     2161 gatagcacag gtccgctctc cttttactga tttgcctacc ctttatatct atgataatta
     2221 tcccggcgga gttggtttta gtgagaaaat ttttgaactt cgcaataaaa ttttaattgc
     2281 tgcccgggaa ttaattgaaa attgtggttg tgatgagggg tgtccttcct gtgtcggacc
     2341 catcaatcag gttgggaatc agggtaaaca gagcgctctt cttattctaa aggaggcaat
     2401 ccgctgatta tggattttag agaacgtttg gaaaagattg aaaatttgaa aagcaaggtc
     2461 catcaaatta atagtaccca tcagaaaagg agcattgatt attctaaaag cagtggaaag
     2521 gcaaccattg ctgaaatcgt ccctggaaat gtaatagata caccccatgg ctccgttttt
     2581 tttgctgagc ataccattcc tttagacaca tcttatggta atacttcact gctggatata
     2641 caaaaagtct tcccagcagg tttacatatc tggcttcccc aattggaaag agagaataat
     2701 gaccttaaac tggatgaaat actatttttt gatactgaga caaccggtct ggcaggaggc
     2761 tcagggacct atatattttt attaggtctc ggttatttta aggaagaatc ttttgttgtt
     2821 aggcagtatt ttatgtcaga ctatcatgaa gaagaagcat tattatgggc tgttaatcag
     2881 cttttttccc gggatttcaa gttgctggtc tcctataatg gtaaagctta tgattaccct
     2941 ttacttcaga cccgttatat aatgatgcgt caaccctttc aactgaatgt ttcttgtcat
     3001 ctcgacttac tttttcctgc cagaagactg tggaagagga gattgttgga ttgttcatta
     3061 tctaatatag agaaaagaat actgaaaatt tatagaaatg aagatatacc gggatatctg
     3121 atacccaatg tttatttccg ttatgtacag aataaagatg cccgctctct caagcccatc
     3181 tttagccata atctgcagga tataatttca ctggtcttac ttaccaccaa gattagccag
     3241 actttagacg atcctctgtc tatggaacaa tttgctctcg atttatgtag tattggaaag
     3301 atttatgagg gatataagga tttccaatac agcagcaggt gttatgaaga ggctttaaag
     3361 ggtaacttat ctgaccagga gatgctggat actttaaaac tatgctcttt tgcctacaaa
     3421 aagcagggag aatgggaaaa agcggaatcg tcttggtatg atattatctc tttaagtagt
     3481 caatttattc ttcaccctta tgaagagctg gcaaaatatt atgagcataa gctgaaagat
     3541 tttcatcagg ctgttagtat agtggacgag gcattgtcaa gattgaaaaa ggagaacaat
     3601 atctcctgga atgttaaaac acaatggcaa aacagtctct ctaaacgatt ggatagaata
     3661 aaaaagaaac aggaattatt gaaagcaaag aatgaaaata aaaatgctga atagatgagt
     3721 tcgcaaatat ggaagaaaac agaaaaaagt atactatcaa ggactggccg gaaaatgaaa
     3781 gaccaaggga aagattattg aattatggac ctgaatatct aagcgatgca gagttactgg
     3841 caattttaat caacaaaggt tatgccggaa aaacttcagt agagatagcc agaagtctgc
     3901 tgacccagtt tggcaccttg agaaatataa tgagtgcctc ctatactgag atgaaaaaag
     3961 taaaaggtat aggaaaggca aaatatgctc aaataaaagc cgcactggaa atgggggtaa
     4021 gattatcaca ggaaaagata ttacctggta gaaaaataca gaaagccgag gatgtgatag
     4081 attattactc tgtagaaatg aaagataaga aaaaagaact ttttcatatg ctcctgctgg
     4141 atgtccgtta tcggattatt cgtgatattc tcatttctat gggaagtttg agtgaaacca
     4201 ctgtccatcc ccgggaagta ataaaagagg tggttaaaga atcagctgct gcagtcattt
     4261 ttcttcataa tcaccccagc ggcaacccgc aacccagtca gcaggaccta gaattgacta
     4321 aaagattgtg tgaatcctgt aatatgttag gagtgaaggt tttagaccat attattgtcg
     4381 gtgataattg tttttatagc tttgcccaga tggggcaaat ataagaaaaa agaaaagggt
     4441 ttttacctaa aacaaaaatg aggaatagag gagaacagaa tgaagcagga tgatttaaaa
     4501 tattgtacaa agtgtggtca tctcttaaaa agtctaataa ttcaagggaa gaaacgtatt
     4561 tattgtcaca attgtgacac catatattat gacaacccta ttcccagtgt agcggtagtg
     4621 acttgtaacg aaaaagggca gttgcttttg gtaaaaagaa aagaagagcc cgggaaaggc
     4681 ttctggtctt taccaggggg atttatggat gatggagaat cagctattca ggcagccctg
     4741 agagagttgc aggaggagac cggtttgcag ggtatagtga aaaggttcat aaaaatattt
     4801 aatcagaata gtgataccta tggacatgtc attattataa cctatgaggt gggtattatc
     4861 ggtggacaac tgaaagctgg tgatgatgct gaaaacgcct gtttttttga tattgaagat
     4921 ttaccactat tagctttctc atttcaaaaa caagctattg aacaggttat aggctgttct
     4981 ttacctgaag caggaactgt ttaagtatga aggtattggc tatagattat gatggtgtga
     5041 ttgtggatag tgtgttggat tctctttttg tcagtcataa cgcct
//
LOCUS       DAOQWT010000041         5048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_16867_length_5048_cov_3.211096, whole genome shotgun sequence.
ACCESSION   DAOQWT010000041 DAOQWT010000000
VERSION     DAOQWT010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16867_length_5048_cov_3.211096"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PKJ95_01500"
     CDS             complement(<1..438)
                     /locus_tag="PKJ95_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNR64948.1"
                     /translation="MNNKYFAEPFKIKMVEPITILTREERLAKIKEANYNLFSLKSED
                     VYIDLLSDSGTGAMSTNQWAGMMNGDEAYSGSKNYFNLISSAQDIFGYEYIQPVHQGR
                     AAEKVVMPILLGPGKFAISNMHFDTTRAHVELTGARAIDCIVPE"
     gene            complement(667..1344)
                     /locus_tag="PKJ95_01505"
     CDS             complement(667..1344)
                     /locus_tag="PKJ95_01505"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HNR64949.1"
                     /translation="MLQISEELKEAYPGGCIGEVSFKNVNVDNSGVINLNRAKDELME
                     SLRDNYKDYSRVALIQTEPMKNYVTYYKKFKKTYHVLLQLESVILKGKSIPESYPLVM
                     IMFMSELKNLILTAAHDLDRITLPIGVELSRGDERYNLLNGMEKELPAGDMFMEDREG
                     IISSIIYGPDSRTQIDEDTNRVLYTVYAPPGIEGSLINEHLNDIVHYINMVAPQAEMI
                     KMTFHKL"
     gene            complement(1408..4002)
                     /gene="mgtA"
                     /locus_tag="PKJ95_01510"
     CDS             complement(1408..4002)
                     /gene="mgtA"
                     /locus_tag="PKJ95_01510"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNR64950.1"
                     /translation="MPQAENKKSIFWTSSIQEVFKLLDSSEKGLSEQEAVSRREKYGF
                     NEIKEKEKRSALDIFFSQFKNSLVLVLIAASIIAYFLGDKIDSLVIISIVIINALLGF
                     FQEYHAEKALRALKKYLISETTVLRDGKIISRDAKEIVPGDIVYLAMGDMVPADLRLI
                     KGEELTTDESSLTGESVPVVKNEEVLEQGYTLPQDLYNILFMGTSVVSGSGYGVVFST
                     GKDTFLGKTASFLKSSPDEGDLQKNIKNFSNFLLKIILTMTIFIFIVNALLGKGIFSS
                     FFFALALAVGLTPEALPIIITISLSRGALKMAEQKVVTKKLASVEDFGNIDILCCDKT
                     GTLTEGKMSLSDYLSIEGEKEVKLVLYGLLCNSARSKGKEDGQVFKNPMDKAIWESIP
                     AQNLKSELEKYKIIKKNEFDFKRRRMSVVVENEGKGMLIAKGAMESILTVSKSVLIGG
                     KATPITAEVSKLLSKKILEYEQNGFRILGIATRYLGDVREGENFEEKDIEEKDLNIEG
                     ILLFLDKAKKTVKESLKILQKLKVEEKIISGDSPVITRKICNDVGLAIKEDRVITGEE
                     LTSLQEDEFKEYCQRFNVFTRVTPEQKFMIVSSLKREGHIVGFLGDGINDVPALKAAD
                     IGISVDTASDIAKESADIILLQKSLRVLSMGIMEGRKTFANITKYILNTISANFGNMF
                     TVAFSSLFLKFIPLLPSQILLTNFVSDAPLMTISTDNVDEEFLRRPRRWNIKLIAKFM
                     VYFGIISTIFDLALVLPLILLLKVEPQVFRTAWFYESVLSEILITFSIRTRLLFARSK
                     PSNLLIWTSLFAVIVVGVLVNTTPGNELFEFVKLPVNVIFLISGILMAYFIAVEIAKK
                     NFFQKFEL"
     gene            complement(4113..>5048)
                     /locus_tag="PKJ95_01515"
     CDS             complement(4113..>5048)
                     /locus_tag="PKJ95_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNR64951.1"
                     /translation="FLSFFILIAFTIFIVLHFTEAEKFAQLLSKAEPKWLVLAIVLQI
                     GTYFCAGFIWNMVIRNAGHQLPLSILARLSIEKLSIDQLVPTGGMSGNLIVVRTMKRF
                     GLPNWLAIEAVVIDIFSHYITDMIMAVLALSVLWFNHNITNIVWYLVAIFTVIVIVVP
                     TSIFWILTHRNGQLPSWLLRFKFITNISETVKMVSPERILLPNLLGGSTLLNMAIFVL
                     DACTLWAMLRITNYSTGILTALVAIVLATIAGAISFLPGGMGGFEAGSVMALALFEVP
                     IEIALTSTLLFRGFTLWIPLIPGVLMTRYDIKEKV"
BASE COUNT         1621 a         1048 c          741 g         1638 t
ORIGIN      
        1 ctcgggaact atacaatcta tggcccttgc ccctgtcagt tctacatgtg ccctcgttgt
       61 atcaaaatgc atattggaaa tggcgaactt tcctggtcca agtaaaatag gcataaccac
      121 tttttctgcc gctctccctt gatggactgg ctgaatatat tcataaccaa aaatatcctg
      181 agcagaactg atcaagttaa aataattttt agagccggaa taagcctcat ctccattcat
      241 catacctgcc cattgattgg tactcatggc gccggtacca ctatcagata ataagtcaat
      301 atacacatct tcagatttta gagagaatag attataatta gcctctttaa tctttgccaa
      361 cctttcttct ctggtcaata tcgtgatagg ctctaccatt ttaatcttaa atggttcagc
      421 aaaatattta ttattcatac tttctgtgac ctccaaatat tatttttaat aataacatta
      481 ttacatttaa ctttccaccc acttctacct gtagaaaatg ccaatttaca gcttgatttt
      541 aacaaattta tcatagatat ccctattccc aatcttctat tcaataatta atatgatcaa
      601 tataaaaaca acttactaat aaaaaatgat ctttaacaaa ataattgaat gaacttcttt
      661 acaattttat aatttatgaa atgtcatttt tatcatttcc gcttgaggtg ctaccatgtt
      721 tatgtagtga actatatcat taagatgttc atttattaga gacccttcta tccctggcgg
      781 tgcatatact gtgtataaca ctctattggt atcttcgtca atctgtgtcc tactatcggg
      841 accatagata atactggaaa tgataccttc cctatcttcc ataaacatat ccccggcagg
      901 taattccttt tccataccgt ttaataggtt atacctttca tctcctctgg acaactctac
      961 tccaattggc aaggtaattc tatccagatc atgggctgct gtaagaataa ggttttttaa
     1021 ttctgacata aacataatca ttaccagagg gtagctttct ggaatagatt ttcctttcaa
     1081 aataacagat tccaattgaa gcaatacatg gtatgttttt ttgaattttt tataatatgt
     1141 tacatagttt ttcataggtt cggtctgaat tagagccact ctactgtaat ctttataatt
     1201 atctcttaaa ctttccatca gctcatcttt tgctctattc agatttataa ccccgctatt
     1261 atcaacgttt acatttttaa aagagacctc tccaatacac cctccgggat aagcttcttt
     1321 taactcttca gatatttgta acataatatt tccatacccc ttcctcttta tttataaaaa
     1381 aatcaacagg gaaaatttta ttaaaaatta cagttcaaat ttttggaaga agttcttttt
     1441 ggcaatttca acggcaataa aataagccat cagaatgcct gatatcagaa aaatcacatt
     1501 taccggtaat tttacaaatt caaataactc attgccagga gtggtattga ctaaaacccc
     1561 taccactatt actgcaaaaa gagaggtcca aatcaacaaa ttgctcggct tactccttgc
     1621 aaataacagc ctggtcctta tagaaaaggt aatcaatatt tcagataaga cggattcata
     1681 aaaccaggca gtacgaaaaa cctgcggctc aacttttaac aataagatta ggggtaagac
     1741 caatgccagg tcaaagatgg tactgataat tccaaaatag accataaatt tagcaataag
     1801 tttaatattc cagcgcctgg gccttcttaa aaactcttca tctacattat cggtagagat
     1861 agtcataagc ggtgcatcac taacaaaatt ggtcagtaaa atctgcgagg gcaaaagcgg
     1921 aataaatttt aaaaacagtg aggagaaggc cacggtaaac atattaccaa aattagcact
     1981 gatggtattc aggatatatt tagtgatatt agcaaaggtc tttcttcctt ccataattcc
     2041 catagacaag acacgcaaac ttttctgaag aagtataata tctgctgact ctttggcaat
     2101 atctgaggcg gtatctactg aaatacctat atcagctgcc ttcaatgccg gtacatcatt
     2161 gataccatcc cctaaaaaac ctactatatg cccttctctc ttcagactgg agacaatcat
     2221 aaatttttgc tctggcgtaa ctctggtaaa tacattaaag cgttgacaat attctttaaa
     2281 ttcatcttcc tggagcgatg taagttcctc tccggtaata actctatctt cttttatagc
     2341 taacccaaca tcgttacata ttttacgggt aataaccgga ctatccccgc tgataatctt
     2401 ctcctctact tttaactttt gtagtatctt cagagattct ttaactgtct ttttggcttt
     2461 gtctaaaaat aacaatatac cttctatatt gagatctttt tcttcaatat ctttttcttc
     2521 aaaattttca ccttccctca catctcccaa atatctggtg gctattccta atatcctgaa
     2581 accattctgt tcatattcta aaatcttttt actcaataat ttagagactt ctgcagttat
     2641 gggggttgcc ttaccgccta tcaggacaga cttgctaacc gtcaatattg attccattgc
     2701 acctttagca ataagcatcc ctttgccctc attttctact actacactca ttcttcttct
     2761 tttaaaatca aattcgttct ttttaataat tttatatttt tccagctcgg attttaaatt
     2821 ttgagcagga atgctttccc atatagcttt atccatcgga tttttaaaaa cttgtccatc
     2881 ttctttgcct ttactccggg ctgaattgca taataagccg tataagacaa gtttaacttc
     2941 cttctcacct tcaattgaaa gataatcgga aagtgacatt tttccttcgg tgagtgttcc
     3001 tgtcttgtca cagcagagga tatcaatatt tccaaaatct tcaactgagg caagcttttt
     3061 ggtaacaact ttttgttcag ccatcttaag agctcctcta gacaaagata tggttataat
     3121 aattggcaaa gcctcagggg taagccccac tgccaatgct aaagcaaaga aaaaagaact
     3181 aaaaatacct ttgcccagca gggcattgac tataaaaata aagatggtca tagtaagaat
     3241 aatcttaagc aggaaattgc tgaaattttt gatattcttt tgcaaatcac cttcatccgg
     3301 gctcgatttc aggaaagaag cggtcttgcc caaaaaagtg tccttacctg tagaaaatac
     3361 cacaccataa ccagaaccgc ttaccacaga ggtacccata aataatatat tgtataaatc
     3421 ttgcggcagg gtatatcctt gttctaagac ctcctcgttt tttacaacag gcaccgattc
     3481 cccggttaaa gatgattcat ctgtagtcaa ctcctctcct tttatcaatc ttaagtcagc
     3541 cggtaccata tctcccatag ccagatagac aatatcaccg gggacaatct cttttgcatc
     3601 tctactaata attttcccat ctcttaatac agttgtttca ctaatcagat attttttcaa
     3661 agctcttaag gctttttcag catgatattc ctgaaaaaat cctagtaatg cattgataat
     3721 tacaatggaa atgattacca gtgaatcgat tttgtcaccc agaaaataag caattattga
     3781 agcggcaatc agaaccagta ccagcgaatt tttaaactga gagaaaaata tatctaaagc
     3841 agacctcttt tctttttctt taatctcgtt aaaaccgtat ttttctcttc ttgatactgc
     3901 ctcttgctca gaaagtcctt tttctgagct atccaatagc ttaaatacct cctggattga
     3961 cgaggtccag aatattgatt ttttattctc tgcttgcggc attgttatag aaaccttttg
     4021 aattttattt aaaatctact tattaaacga agcaactcat ccatttgttc aattgttaaa
     4081 tattttcttt ataatagtac aacgaaaaga ttctatactt tttccttaat atcatacctg
     4141 gtcatcaaaa cacctggaat aagtggaatc cacagagtaa atccacggaa aaggagtgtg
     4201 ctggttaaag caatttctat cggtacctca aaaagtgcca gagccattac tgaaccagcc
     4261 tcaaatcctc ccataccacc tggtaaaaag gaaatagcac cagctatagt agccagaaca
     4321 attgccacta aagcagttaa aattccggtt gaataattag ttattcgtag cattgcccaa
     4381 agggtacagg catcaagaac aaaaattgcc atatttagta aggttgagcc ccccaataaa
     4441 tttggtaaaa gtattcgctc tggtgagacc attttaaccg tttctgaaat attggtaatg
     4501 aatttaaaac gaagcaacca ggagggcagc tggccattcc tgtgggttaa aatccagaat
     4561 atagaggtag gaactactat tactattaca gtaaaaatgg caacaagata ccatacaata
     4621 tttgtgatat tatggttaaa ccagagaacc gacaatgcca gcacagccat aatcatgtca
     4681 gttatataat gggaaaaaat atcaatcacc actgcctcaa tggcaagcca gtttggtaac
     4741 ccaaatctct tcattgttcg caccactatt aaattccctg acatccctcc ggtgggaact
     4801 aattggtcaa tacttaattt ttctattgat agacgtgcca atatacttaa aggcaactga
     4861 tgccctgcat ttcgaattac catattccat ataaaacctg cacaaaaata agttccaatt
     4921 tgtaatacga tagccaaaac taaccatttt ggttctgctt tagataatag ctgggcaaat
     4981 ttttctgctt cagtaaaatg gagaacaata aagatagtaa aggctatcaa tataaaaaaa
     5041 ctcaggaa
//
LOCUS       DAOQWT010000042         5026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_16948_length_5026_cov_3.568095, whole genome shotgun sequence.
ACCESSION   DAOQWT010000042 DAOQWT010000000
VERSION     DAOQWT010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16948_length_5026_cov_3.568095"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKJ95_01520"
     CDS             complement(<1..194)
                     /locus_tag="PKJ95_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64952.1"
                     /translation="MKKGDDFIRIYREYKFKLISWPPSSTPLNYPKYYEEWQAIEIIH
                     NDYKSGNFVEKKLYYKQISVS"
     gene            complement(191..397)
                     /locus_tag="PKJ95_01525"
     CDS             complement(191..397)
                     /locus_tag="PKJ95_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64953.1"
                     /translation="MDSQINKTLDMIKLIVDIEINKAIEEIYTEVEELSESIASSVSK
                     IANKSLLSTGEIINNEIENRRTIQ"
     gene            complement(567..818)
                     /locus_tag="PKJ95_01530"
     CDS             complement(567..818)
                     /locus_tag="PKJ95_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64954.1"
                     /translation="MMIILENNRRYIWGEIQNSVIDNIEKSQCECNNGKILKFSYTKI
                     NNTWQAICDCGRCFYVTNAENEGANIYPDTIVRVHINKQ"
     gene            complement(815..1087)
                     /locus_tag="PKJ95_01535"
     CDS             complement(815..1087)
                     /locus_tag="PKJ95_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64955.1"
                     /translation="MCKTEVINETKQLIRDIRSLKEEMELFIKEQDKKIAEKKKQIIH
                     NIDFMGKVEDNHKVLDLGRGKKITYPIPEINTLFLFTENIGVELIK"
     gene            complement(1094..2398)
                     /gene="iscB"
                     /locus_tag="PKJ95_01540"
     CDS             complement(1094..2398)
                     /gene="iscB"
                     /locus_tag="PKJ95_01540"
                     /inference="COORDINATES: protein motif:HMM:NF040563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease IscB"
                     /protein_id="HNR64956.1"
                     /translation="MKMVFVVDKNKRPLNMCHSAKARKLLSDNKAVVISYYPFVIRLK
                     KEVNTPIVADYQIKIDPGAKWTGLAILQNEVNVNFLGVLKHKAFEVVDKLKSRSSLRH
                     GRRSRKTRYRQPRFDNRGNARKLGRIMPSLRSRFDNITNWIKKLQKFCPIGEIVYENV
                     KFDTQLLENPDIVGTEYQRGELCGFEIIEYLREKTGFKCAYCGKGGMKEKLEVEHIIP
                     RSRNGSNRISNLTLACHKCNQKKGNMTAKEFGKPEVQGNAKKPLKDTAMVNSSRKRMY
                     EELKQFNLPIRIGSGGRTKWNRIRQGLPKTHWFDACCIGVMGEEKLKLNINVVLEIGC
                     CGRGRYKICDTDKYGFPKAWRGRDKKTGKCHKVYQGFTGQSIIRAEYKGKIVKVLLVS
                     PRMKGSFTMQVGNNKISVSPKKCSYIQRFQGYKLRYITKERR"
     gene            complement(3031..3309)
                     /locus_tag="PKJ95_01545"
     CDS             complement(3031..3309)
                     /locus_tag="PKJ95_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64957.1"
                     /translation="MMEEKDLSEEENNSLFNDLSPDMKLVATHCSLSVALSLQQNLPG
                     IRIDIPKKKEKPLKNKLVRIDYKKGISIKELALKYSICERRVQQIVSE"
     gene            complement(3332..4891)
                     /locus_tag="PKJ95_01550"
     CDS             complement(3332..4891)
                     /locus_tag="PKJ95_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64958.1"
                     /translation="MNSGEMLGKKKLLLMNQKPQSDEQLLWYIEEFLGFHIATKAVCP
                     HHRSPFEFISDMFFNKGEDNKVVLASRDSGKTQDIAIINALNIKFKPGIESAIVGAIS
                     DQAYKCYSYIQEFHNKNFLYNEVINSIMQVSRYKNGAKVEIVVGTATGVNSPHPNIVF
                     FDETDLIKWNILQQGFSMPHASNIYKSQIILDSTRKDTEISTGSMQKLIDNVEDGLMP
                     FTEIYSWCIWEVIEKCYLPNCSQCKKIMRYNEEGKPESFADKCQGKAKLADGHYQIQE
                     VWKKFTTLDYNTFDSEWLCERPVKSGRMLPQFNENIHMIDCEYNPNLPVDSCQDFGFS
                     NPIVTLFAQIDSSDTVYIFDELVEYERTAEELCAENGSWNLKNKEYKPRIWCCDPENP
                     SDRKTMRTHGINEVPVKCGVYESTQLVRKWLRPVSSPKPKLYVSKKCKFLKNELLNWK
                     RRKNSEEGEDKDNHSIGALRYLLWHYFGDNVSSMPEVMTKNPRQSQGLIKGYTEQSYT
                     GIRQSIMKGYK"
     gene            complement(4827..>5026)
                     /locus_tag="PKJ95_01555"
     CDS             complement(4827..>5026)
                     /locus_tag="PKJ95_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64959.1"
                     /translation="PSDQMIKLFLETHNPETYNIKQKVEHSGKLEVDTKNLTLTKDEL
                     DELWRDVRKEKTPIDEPKTSE"
BASE COUNT         1441 a         1011 c          652 g         1922 t
ORIGIN      
        1 gaaacgctta tttgtttata gtatagtttt ttttccacga aattaccact tttgtaatca
       61 ttatgaataa tctctatagc ttgccattcc tcgtaatatt ttggataatt aagtggagtg
      121 gaggatggag gccaggagat tagtttaaat ttatactctc gatatattct tataaaatca
      181 tctccctttt tcattggata gtcctcctgt tttctatttc attattaatt atttcccctg
      241 tagataaaag agatttattt gctatttttg aaactgatga agcaatagat tcacttagtt
      301 cctccacttc agtatatatt tcctctatgg ctttattaat ctcaatatct actattaatt
      361 ttatcatatc taaagtctta ttaatttgac tatccatagg taatatttta ttataatgct
      421 tgtctatatg ctctataata ttcttcctta ccctctccag gctatcttac tatcaggatt
      481 taaaacgaaa cccattgttt cttctacagc accattatta ttagttttac attctaaaat
      541 gactttacta tccataaata ttcctcctat tgcttgttaa tatggactct tactattgta
      601 tcagggtaaa tattagcccc ttcattttcc gcattagtaa cataaaaaca tctgccacaa
      661 tcacaaatag cttgccaggt attattgatt ttagtataag aaaatttaag gatttttcca
      721 ttattacact cacattgaga tttttcaata ttatcaataa ctgagttctg aatttccccc
      781 cagatatatc ttctattgtt ttctaatatt atcatcattt tattaactca acccctatat
      841 tttcagtaaa taaaaataag gtattaatct caggaatggg ataagtaatt ttcttccctc
      901 tccccagatc taatacctta tggttatctt ctactttacc cataaaatca atattatgaa
      961 ttatctgctt ctttttctct gctattttct tatcctgctc ttttatgaat aattccatct
     1021 cctctttaag cgaccttata tctctgataa gttgtttcgt ttcatttata acttcagttt
     1081 tacacatagt taattatctc ctttcttttg tgatatacct taatttatac ccttgaaacc
     1141 tctgaatata actacatttt ttaggactta ctgatatttt attattaccc acttgcatag
     1201 taaaactacc cttcattctt ggactaacta acaacacctt gactatcttt cctttatatt
     1261 ctgcccttat aatactttgt ccagtaaatc cttgatatac tttatggcat ttacctgttt
     1321 tcttatctct tcccctccac gctttaggaa atccatattt atctgtatcg catattttat
     1381 accttcccct gccacagcaa cctatttcca atactacatt aatatttaac tttaactttt
     1441 cttcacccat aacacctata caacaagcat caaaccaatg tgttttagga agcccctgtc
     1501 gaattctatt ccacttagtt cttccccctg aaccaattct tataggcaag ttaaattgtt
     1561 ttaattcttc atacattctt ttacgagaac tatttaccat tgctgtatct tttaaaggtt
     1621 ttttagcatt accttgaact tcaggtttac caaattcttt agcggtcata tttcctttct
     1681 tttgattgca cttatggcaa gccaaggtta aattacttat cctattgctt ccattcctgc
     1741 ttcttgggat aatatgctcc acttctaatt tttccttcat tccccccttt ccacaataag
     1801 cacatttaaa gcccgttttc tctcgtaagt attcaattat ctcaaatcca cataactctc
     1861 ctctctgata ttcagttccg actatgtcag gattttccaa taactgagta tcaaatttaa
     1921 cattttcata aacaatttct cctatagggc agaacttctg taacttctta atccaatttg
     1981 taatattatc aaaccttgat cttaaagaag gcattattct acctaatttt ctagcgtttc
     2041 cacgattatc aaatctcggt tgcctgtatc ttgtttttct gcttctcctc ccatgcctta
     2101 aagagcttct ggattttaac ttatccacta cctcaaatgc cttatgtttt aatactccta
     2161 aaaagttaac attaacttca ttctgcaata ttgccaaacc tgtccatttt gcaccgggat
     2221 ctatttttat ttgataatct gctactatag gagtattaac ttctttcttt aaccggataa
     2281 caaaaggata ataagaaatt actacagctt tgttatcaga caataacttc cttgcctttg
     2341 cagaatgaca catatttaaa ggtcttttat tcttatctac cacaaacacc attttcattt
     2401 actccttatc ttttcagata atctctgttg tttatttata tcaacaggta acgtacttct
     2461 ttcgaaatat ctcccctcgc caatgttagg tatgcttaca taaatattat actgtagtct
     2521 caatcaagac ctcacttaat aatacttgac agttgctacg aactgaggaa gtatccgtag
     2581 gtgtcataac atttctaacg tagtcactcg acttatggct ggttacatag tgagagtttt
     2641 cactctcgaa tttgtgaata gtagttttcg aaaatcggct atagttttct acaagaaagt
     2701 agtttgcgaa aaatagttta aaaactactt tttgacactt agattttacg ccatttttta
     2761 ctactgtttt catcggtttc atctctactc ctcgcaaatt gattgttgta actcacctgt
     2821 ggacagtaga ggcagaattg agcactattg tttcatctct actcctcgca aattgattgt
     2881 tgtaacttcc ctataattaa aaaattttcc cttagttcct acctctttaa atcttctgtt
     2941 tgccgtagaa gtgggtaaga actaagggaa aacttctata aacttctacg gcttttttat
     3001 tatatcaaat caggaaataa aaatcaaata ttattcagaa acaatttgtt gaacccttcg
     3061 ctcacagata gaatatttta gagcaagttc tttaatagaa ataccctttt tgtaatcaat
     3121 tcttacaagc ttgttcttta atggtttttc tttctttttt ggtatgtcga tacgaatacc
     3181 cggaagattt tgctgtaagc tcaaggccac agataagcta caatgagtgg caaccagttt
     3241 catatctgga ctaagatcgt taaataaact attgttttct tcttctgata aatccttttc
     3301 ttccatcatt ttcacataac atctccttct cttacttata acccttcata attgactgcc
     3361 ggattcccgt ataagattgt tcagtatatc ctttaattaa tccctgggat tgtcggggat
     3421 tttttgtcat tacctctggc atacttgaaa cattatctcc gaagtaatgc cagagtaaat
     3481 atcttaatgc ccctatacta tgattatctt tatcttctcc ctcttcagaa ttctttcttc
     3541 ttttccagtt taataattcg ttttttagaa acttacattt tttagaaaca tatagttttg
     3601 gtttgggaga agatactggt cgtaaccatt ttcttactaa ctgagtactt tcatagactc
     3661 cacattttac cggaacttcg tttataccat gagttctcat ggtttttcta tctgaggggt
     3721 tctctggatc acagcaccat atacgaggtt tatattcttt gttctttaaa ttccatgatc
     3781 cgttttctgc acatagttct tcagcagtcc tttcatactc aaccagttca tcgaaaatat
     3841 atacagtatc agaactatct atttgagcaa ataaggtaac aataggatta ctaaatccaa
     3901 aatcctgaca agagtctacc ggtaaattcg gattatattc acaatcaatc atgtgaatat
     3961 tttcgttgaa ttgtggaagc atacgaccag attttacagg tctttcacat aaccattcac
     4021 tatcaaaagt attataatcc agagtggtga atttcttcca tacctcttgt atttgataat
     4081 gtccatctgc cagctttgcc ttaccctgac atttatctgc gaagctctca ggttttccct
     4141 cttcattata tcgcattatt tttttacact gagagcagtt aggtaaataa catttctcta
     4201 taacttccca gatacaccag gaatatatct cagtaaatgg cataaggcca tcctcaacat
     4261 tgtcaataag tttctgcatt gagccggtag aaatttcagt atcttttctt gtggagtcca
     4321 gaatgatttg agatttatat atattacttg catgaggcat agagaaaccc tgttgaagaa
     4381 tattccactt aatgagatct gtttcatcaa aaaatacaat attaggatgg ggggaattta
     4441 cacctgtagc agttcctacg actatttcaa cttttgcacc attcttgtat ctcgaaacct
     4501 gcattattga attaattact tcattgtaga ggaaattctt attgtgaaac tcctgaatat
     4561 agctataaca tttgtaagcc tgatcactta tagcccctac aatagcagac tctataccgg
     4621 gtttgaattt tatatttaaa gcgttgatta tagcaatatc ctgagtttta ccactatcac
     4681 gggaggccag cactacttta ttatcttccc ctttattaaa gaacatatct gaaataaatt
     4741 caaaaggaga tctatgatgg gggcatactg ctttagtggc tatatgaaaa ccaagaaact
     4801 cttctatata ccagagtaat tgttcatcac tctgaggttt ttggttcatc aataggagtt
     4861 ttttctttcc taacatctcg ccagagttca tctaattcat cctttgtgag agtaagattt
     4921 tttgtgtcaa cttccaattt gccggaatgt tccactttct gcttaatgtt ataagtttcc
     4981 gggttatgag tttcaagaaa tagcttaatc atttggtctg aaggtg
//
LOCUS       DAOQWT010000043         5018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_16978_length_5018_cov_3.411042, whole genome shotgun sequence.
ACCESSION   DAOQWT010000043 DAOQWT010000000
VERSION     DAOQWT010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16978_length_5018_cov_3.411042"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKJ95_01560"
     CDS             complement(<1..661)
                     /locus_tag="PKJ95_01560"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNR64960.1"
                     /translation="MLFIAVSLIAFFISYIITPFIALLGKKQNLVDKPGYRKIHNNAI
                     PTLGGIVIFFGFLFSVLFLVPLQRQEQVLLAGSIIILLLGVVDDIADLKPQIKFGIQM
                     IPALLFIAFNNNLINQFIIEKLSSLNIIGYLLYPILILWIVGITNSINLIDGLDGLAS
                     GISIIALSTFLISGFFINKGYQIYNLLNSALLGSTVAFFRYNFFPAQIFLGDSGSTFA
                     GF"
     gene            complement(778..1914)
                     /locus_tag="PKJ95_01565"
     CDS             complement(778..1914)
                     /locus_tag="PKJ95_01565"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR64961.1"
                     /translation="MAKKEKIMKKNDKNKKNWFDIIFYTLSAIVIIVLLFIGLRQRGW
                     LPIFWIDEKPSSQIAAIPQEIKERRANFNIINAHEHVQSEKNISLIRKTMKDCQIKKM
                     VLLGTSDFTFFLNSSYGFTGYEENNEFVIKCSQDYPDEFAALVTLDPRDENKMEKLQN
                     YIKAGAAGVKLYNGHGSFYDLFFHMPLIDSSMMEIYSYCEKEHIPILYHINIGRFSEE
                     FEKILKEFPQLVIIAPHFMLSTNSLNRLDRFMKEYPQLYLDISFGHPDFLVSGFSRIS
                     NNYIAFHDFMVNYRDRITFGTDLVVTTYKAKNRTYIDDIHLAYMDLLEKDDFVLPSSI
                     YNMLSKDVRKNTDTTRIFKGLNLDDVTLNAIYHDNAEKLFFKDK"
     gene            2049..>5018
                     /locus_tag="PKJ95_01570"
     CDS             2049..>5018
                     /locus_tag="PKJ95_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64962.1"
                     /translation="MFRYKIIYKKGSLLSLIFPLSLVIFLTLLNCVFYNHLSLEVHAN
                     VYDPSLYWKTLDSKYCSIIFPLPVLDKHTTQYQEIAIHIAEIVDEIYPQITIQFGKPF
                     HSHKKFTIILEDFSDSTYGFATTMPHPLIRINLTAPGFNIFDTKFESWLRILIAHEYT
                     HLAQFDMTDKFTTFMRLFLGQIIAPNALQPVWSIEGLAIYNESKLTSGGRLQDSRYEM
                     YLRADFLENQVKKLEQIEGDYLVSWPGGNAPYIYGQSLVHFISNEFGEEKLIKISRIF
                     SSLPLLGMNWSLKKVLGIGMDELFQKWKNEKKRQYEQQIDEIINYSDITKSQQITNHH
                     YWVGNPIWLTDKELRNPILFYKVFTPQLYPTIRRYDLNSGAESILIKRTSGHGSSYSL
                     SPDKQYLLYSKLIQYSQYYNYYDLFLYNFNTGKQYQVTEKMRIKDPAWHPDSSTGKVA
                     AVINKAGTNNLVLISFNDLSKLKNKHNLDELTSFPNLIFLTDFNDSTQISQPVWSPKG
                     DMIALSIWHQGYQDIYIITLDEQNIIKSIKPITLDHYTDINPNWSPDGQYLLFSSDRS
                     SVFNLYAYSLLDNQFYRLTNVTTGVFEPAVSPDGNKIAFIQYHSSGYELHLAEINHLL
                     WKLIDKPRMNLSANYPTIKDNKIAFLKTLESKNDEDDQLNTSGFSSNLYSSYSFKDYS
                     PQDSIMPTYWIPYASISDKNFYLGFSTTAQDYLKFYEMPITIAYNLFNDSLFYDFQHF
                     NYSYKPVFSFSWQGETSSFSELQLSLRFTDSGYTSVQDSGRFYSRSINIGIQNKYFLD
                     INNHLDKGRNGDTKVLNSLILKYNYSDAERYTASIGPQIGNSFSLNYQYAPASLNNNQ
                     YFYKILFDGRKYFPLSLKNQVLALRLVAGFVSNELNYEAKFNLGGNFTASSLSSTKTD
                     SFSLRGFPDSSFVGNHLLLTSLEYRFPLKMVEDKIGFKWASVFLEQVSGKLFLDVGNA
                     WDKKILPTIQDINISIGA"
BASE COUNT         1701 a          862 c          742 g         1713 t
ORIGIN      
        1 aaaaaccagc aaaggtacta ccggaatcac ctaaaaatat ctgcgccggg aaaaaattat
       61 agcgaaaaaa agcaacagtg ctgcctaata atgctgaatt tagaagattg taaatctgat
      121 aacccttgtt gataaagaac cctgatatca aaaaagtaga taaggcaatt atagaaatac
      181 ctgatgccaa accgtctaat ccatcaatta aattgataga attagtaata cctacaatcc
      241 ataaaatcaa tatgggatat aaaagatagc ctataatatt taatgaggat agtttttcta
      301 taataaattg attaattaaa ttattattga aggcaatgaa aagaagagct ggtatcattt
      361 gaataccaaa ttttatttgg ggtttcaaat cggcaatatc atccacgact cctaataata
      421 aaatgataat actcccggct agtagcacct gttcctgcct ttgtaaaggt actaaaaaca
      481 gaacactaaa taaaaaacca aaaaatatta ctatacctcc cagagtagga atagcattat
      541 tgtgtatttt acgatatcca ggtttgtcta ccaaattctg ttttttaccc aataaggcga
      601 taaatggagt aattatatat gaaataaaaa aagctatcag cgatactgca ataaaaagca
      661 ttgacggctc ttttctcctc ttctttgaat atttttatta caagcaatgt aatgtaattg
      721 aatatttttg ggatattcta aaaatattca atcatcagtt attttttatc aaaggactta
      781 tttatcttta aagaaaagct tttcagcatt gtcatgataa atcgcattta gtgtcacatc
      841 gtcaaggttt aaacctttga aaattcttgt ggtatctgta tttttacgaa catctttaga
      901 tagcatattg tatatgctgg aaggaaggac aaagtcatct ttttccaata aatccatata
      961 agccagatgt atatcatcaa tatatgttcg gtttttggct ttataggtag ttacaactaa
     1021 atcagttcca aaggttattc tgtcccgata atttaccata aaatcatgaa aagcaatata
     1081 gttgttggat attctactga aaccagaaac taagaaatca ggatgtccaa aactaatatc
     1141 aagatataac tgaggatatt ctttcataaa acgatctaaa cggtttaaac tattggttga
     1201 tagcataaaa tgaggtgcta taataactaa ttgtggaaac tcttttaaaa tcttctcaaa
     1261 ctcttctgaa aatcgaccga tattaatgtg atataagatg ggaatatgtt ctttttcaca
     1321 atacgaatat atttccatca tactagaatc aatcaatggc atatggaaaa ataaatcata
     1381 aaaactacca tgcccgttat acagctttac cccagctgct ccagctttta tataattttg
     1441 taatttttcc attttatttt catctctggg gtcaagagta accagagctg caaactcatc
     1501 cggataatct tgactgcatt taataacaaa ttcattattc tcctcataac cggtaaaacc
     1561 atagctactg ttcaagaaaa aggtaaaatc cgaagtacct aataatacca tttttttaat
     1621 ctgacaatct ttcatagttt tcctaatgag agatatattt ttttcacttt gcacatgttc
     1681 atgtgcattg ataatattaa aattagccct tctttcttta atttcttgtg gaatagccgc
     1741 aatttgagag gaaggtttct catcaatcca aaatatcggc agccagcctc tctgtctcaa
     1801 gccgataaac agtaatacaa taatgacaat ggcacttagg gtataaaaaa taatatcaaa
     1861 ccaatttttt ttatttttat catttttttt cattattttt tctttttttg ccaagaattg
     1921 attctccttt ctatgtgaaa tttttttcat tatacccgat tcatagtcct atttccaaag
     1981 ttttataaat ttattgtaac tactttaaaa taaagttata attttgatga agttgctaaa
     2041 ggtgaagtat gttcagatat aaaatcattt ataaaaaagg ctctctttta tcccttattt
     2101 ttcccctatc tttagtaata ttcttaacat tactaaattg tgttttttat aatcacctat
     2161 cgcttgaagt tcacgccaac gtttatgacc cttccctcta ctggaaaaca ttggatagta
     2221 aatattgctc tataatattt cctttaccag ttttagataa acatacaact caataccaag
     2281 aaattgctat tcacattgct gaaatagttg atgagatata ccctcaaatt accatacaat
     2341 ttgggaaacc ttttcattcc cataaaaaat tcactattat tttagaagac tttagcgatt
     2401 cgacttatgg cttcgccacc accatgcctc atcccctaat ccgtattaat ctgactgctc
     2461 caggctttaa tatttttgat actaaatttg aaagctggct tagaatactt atagctcatg
     2521 aatataccca tttagctcaa tttgatatga ctgataaatt tactactttt atgcgccttt
     2581 ttttgggtca aattattgct cccaatgccc tgcagcccgt atggtctatt gaaggattgg
     2641 caatttacaa tgaaagtaaa ttaacctccg gaggacgtct tcaggatagt agatacgaga
     2701 tgtatttaag agctgatttt ttagaaaatc aagtgaagaa acttgagcaa atagaaggag
     2761 attacctggt atcctggccg ggaggaaatg caccttatat ttatggccaa tctttagttc
     2821 attttataag taatgaattt ggggaggaaa aattaattaa aattagcagg attttttctt
     2881 ctttgccatt gctgggaatg aattggagtt taaaaaaagt cctaggaatt ggtatggatg
     2941 agcttttcca gaagtggaaa aatgaaaaaa agaggcaata cgagcaacaa attgatgaaa
     3001 taattaatta tagcgacatc accaaatccc aacaaattac caatcaccac tactgggttg
     3061 gtaacccaat atggttaaca gacaaagagc tccggaatcc gattcttttt tataaggtct
     3121 ttacaccaca actctatccc acaattcggc gttatgacct taattctgga gcagaaagta
     3181 tcctaataaa aagaacatca ggacatggca gttcttactc tctgtcgccg gataagcaat
     3241 atttactcta ttctaaattg attcaatatt cccaatatta taattattat gacctttttt
     3301 tatacaattt taacactggc aagcagtatc aagttacaga aaaaatgaga attaaagacc
     3361 ccgcctggca tccagattcc tctacaggaa aggtagctgc tgtgataaac aaagctggaa
     3421 ccaataacct ggttttaatt tcttttaatg atttgagtaa acttaaaaat aaacataatc
     3481 ttgatgaatt aacttctttc cccaatctta tttttctgac tgattttaat gatagtactc
     3541 agatttcaca acctgtttgg tcaccaaaag gtgatatgat tgccctttct atatggcatc
     3601 aaggatacca ggatatttac ataattacct tggatgaaca aaatataata aaatcaatca
     3661 aaccaattac cctggatcat tatacagata ttaacccaaa ttggtcgcca gacggccaat
     3721 accttttatt ctcatctgac cgaagtagtg tatttaattt atatgcctat tccctattag
     3781 ataatcaatt ctatcgctta actaatgtaa ctaccggtgt ttttgaacca gctgtctctc
     3841 cagatgggaa taaaattgct tttattcaat atcactcctc agggtatgaa ttgcatttgg
     3901 ctgagattaa ccatctgctc tggaaactaa ttgataaacc tcgtatgaat ttgtcggcta
     3961 actatcctac aattaaagat aataaaatag cttttctaaa aacattggaa agtaaaaatg
     4021 atgaagatga tcagttaaat acatccggtt tcagttcaaa tctttactca tcttattctt
     4081 tcaaagacta ttctcctcag gatagtatta tgcctactta ctggattcct tatgccagca
     4141 tttcagataa aaacttttac cttggttttt ctacaacagc tcaagattat ttaaaatttt
     4201 atgaaatgcc tataacaata gcctataatc ttttcaatga ttctttgttt tatgattttc
     4261 aacattttaa ttacagttat aaacctgtat tttctttttc ctggcaagga gaaacatctt
     4321 ctttttctga gttacaactc tcattaagat ttactgatag cggctatact tctgtgcagg
     4381 attccggtag attttattct cgaagtatta acatagggat acaaaataaa tactttttag
     4441 acattaataa tcatttagac aaaggacgca atggtgatac aaaagtactt aattccctga
     4501 ttttaaaata taattatagt gatgctgaaa gatatactgc atccataggc cctcaaatag
     4561 gcaattcttt ttcactcaac tatcaatatg ctccagcaag cctgaacaat aaccagtact
     4621 tctataaaat tctttttgat ggcagaaagt atttcccttt atctttaaaa aaccaggttc
     4681 tagcgttgcg gctggtagct ggttttgttt caaacgagtt aaattatgaa gcaaagttta
     4741 atcttggtgg aaactttact gcctctagtc ttagttcaac aaaaactgac agtttttctt
     4801 taagaggatt ccctgattct tcttttgtcg gtaatcattt attgctgact tctctggaat
     4861 ataggtttcc cttaaaaatg gtggaggata agattgggtt taaatgggct tctgtttttt
     4921 tagaacaagt ttctggaaaa ttatttttag atgttggaaa tgcctgggat aaaaaaatat
     4981 tgcctactat tcaggacata aatatctcta ttggtgct
//
LOCUS       DAOQWT010000044         4877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_17650_length_4877_cov_2.836375, whole genome shotgun sequence.
ACCESSION   DAOQWT010000044 DAOQWT010000000
VERSION     DAOQWT010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17650_length_4877_cov_2.836375"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..2228)
                     /gene="fusA"
                     /locus_tag="PKJ95_01575"
     CDS             complement(78..2228)
                     /gene="fusA"
                     /locus_tag="PKJ95_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNR64963.1"
                     /translation="MSIKEKKQSINFEKFIKKDIEKRHSMLNSNSLQQVRNIGIMAHI
                     DAGKTTVTERILYYTGRVHKMGEVHEGTATMDWMPQERERGITISSAVTTSFWKGFQI
                     NTIDTPGHVDFTVEVERSLRVLDGAIVVFCAVAGVEPQTETVWHQADRYQVPRIAFVN
                     KMDRIGADYYGTIKMIREKFATIPLITQIPWGSESNFQGIIDIIKMKAYSYTLDSLGT
                     NYCIEEIPDKYKKEAEKAQKNLMDILSEEDEKFMEDYLDGNEISEEKIHQTIRRLTIK
                     NKVVPVLCGSALKNKGIQFLLDAVNSYLPSPMEVLPIKGENPKTGKEIIRETDVNEPL
                     SVLVFKVMTDPFFGRLCFIRVYSGIIKEGCYVYNSTQDVKERINRLVKIHADHKEQVK
                     EITAGNLGAIIGLKKSGTGDTLCDENNPIILEKIKFPEPVISIAIEPKSFTDRDKMAI
                     ALEKIAEEDPTFKHSVNKDTGQTIISGMGELHLEIIVDRLLREYRLDGNVGKPQVAFK
                     ETITKKATAQGKFIRQSGGRGQYGDVIIEIEPFKEGNFEFIDRTVGGTIPKEYMPAVK
                     QGIQEAMLCGVLASYPVTNIKVSVVNGSFHPVDSSELAFKIAASKALKEGLKKASPVL
                     LEPIVEIEIRTPEEYLGSIIGDLNSRRAKILGIQEKKNIKGKIILAHVPLQEVFGYAT
                     NLRSITQGRAIYVMQFAYYEVVPENIIKKISYKI"
     gene            complement(2244..2714)
                     /gene="rpsG"
                     /locus_tag="PKJ95_01580"
     CDS             complement(2244..2714)
                     /gene="rpsG"
                     /locus_tag="PKJ95_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNR64964.1"
                     /translation="MSRKRKAIKREINEDPVFQDKRVSKFINHIMWDGKKNIARNIFY
                     DAMDIVKEKTKKDSLTVFNQAMENVMPILEVKSRRVGGASYQVPIEVSPERRVTLAMR
                     WIINFSRSKTGKPFSEKLAAELIEASQGAGASVKKKEDTHKMAEANKAFAHYRW"
     gene            complement(2789..3163)
                     /gene="rpsL"
                     /locus_tag="PKJ95_01585"
     CDS             complement(2789..3163)
                     /gene="rpsL"
                     /locus_tag="PKJ95_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNR64965.1"
                     /translation="MPTINQLVKKGRKKVIKKSGTPALKGSPLKRGVCTRVWTITPKK
                     PNSALRKVARVRLTNNMEVTAYIPGIGHSLQEHSIVLIRGGRVKDLPGVRYHIVRGVM
                     DATGVEGRKQSRSRYGTDRPKK"
     gene            complement(3280..>4877)
                     /locus_tag="PKJ95_01590"
     CDS             complement(3280..>4877)
                     /locus_tag="PKJ95_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNR64966.1"
                     /translation="GFIDPNDILRIQGIKAVQKYLLEQVQEVYRSQGVTINDKHIEVI
                     VRQIARLNKIYIRSSRDSDLLSGELVYVSDFEQANKKVAQENEEIRNKNRELLLNKTL
                     VNCLTGSKSESIIDKNTIIDENIIKKIENSDCQSFDILDESGNLDNIIQGEINFIERI
                     KDHIFIDDISKNSLNILNTNHNESDNLQKLIGSKITKETALELMKNGTNRIRIFNPDI
                     KIKVLGKMIAQDIKDRDEQNVIVYANTILNENDIEKLLEHQCKEILVWSDIKEINIKE
                     NLLNLIKEDVIGKKLGEELRDSKTGNVIADMGQLISKNIIRKAYSAGISDIPLEGGHF
                     FSLEGRTLDYIYHNLIGKIIAQDIIDSNSGVTLLSAGERITKNNANIFFQQNIELVKY
                     RTEQSLQEPILLMENIGFMKRIKLPAVGMPIIQGITQASLSTESFLSGASFQRTTHVL
                     TNASIKGKVDYLCGLKENVIIGNMIPAGTGLPKYREVEITSQYEKELESEDIKESIEE
                     NIVVKQENNIINNQENLNEENQN"
BASE COUNT         1383 a          954 c          650 g         1890 t
ORIGIN      
        1 ggcttggagc ggacaaactt ttctttagcc attttgtttt ttcctcctta aaaggcaaac
       61 ttttagtttt tatagtttta tattttatat gatattttct ttataatatt ttcagggaca
      121 acttcataat aagcaaattg cataacataa atagccctgc cctgtgttat tgatcttagg
      181 ttagttgcat aaccaaaaac ttcctgtaag ggaacatggg ctaaaattat cttgccttta
      241 atattctttt tttcctgaat tcctaatatt ttagctctac gtgaattcaa gtcgccaatt
      301 atagagccta agtattcttc aggagttcta atctctattt cgacaattgg ttctaaaagc
      361 actggactgg ctttttttaa tccttctttt aaagctttag aggcagcaat tttaaaagcc
      421 aattcagaag agtccactgg atggaaagaa ccattaacca cagatacttt aatattggta
      481 acagggtaac tggccaatac accacataac attgcttcct gtatcccttg ttttacagcc
      541 ggcatatatt cctttggaat agttcctcct actgttcggt caataaattc aaagttacct
      601 tctttaaatg gttcaatttc aataattaca tcaccatact gacctcttcc gccactctgt
      661 cttatgaatt tgccctgggc tgttgctttc ttcgtaattg tttctttaaa ggctacctgt
      721 ggcttgccta cattcccatc aagtctatat tctcttaata accggtcaac aataatttct
      781 aaatgtagtt ctcccatacc ggaaattatg gtttggccgg tatctttatt gactgaatgt
      841 ttgaaagtcg ggtcttcttc agcaatcttc tctaaagcga tagccatttt atctctatcc
      901 gtaaaacttt tcggttcaat ggcaatagat ataacaggct cggggaattt aattttttcc
      961 aatataatgg gattgttttc atcacataaa gtatcaccag ttccagattt cttaagacca
     1021 ataatagctc ctaagtttcc agcagtaatt tctttaacct gctctttatg gtcggcatga
     1081 atcttaacta atctgtttat tctttcttta acatcctggg tagaattgta gacatagcag
     1141 ccttctttta ttatgccaga atacacccga ataaaacata atctcccaaa aaaagggtca
     1201 gtcattactt taaaaactaa aacagacaaa ggttcattga catctgtttc ccttataatc
     1261 tctttacctg tttttggatt ctctccttta attggtagca cttccatagg cgagggaaga
     1321 taactgttta cagcgtctaa aagaaactgt atccccttgt tttttagagc agagccacat
     1381 aggactggga ctactttatt ctttatagta agtcttctta tggtttgatg tatcttttcc
     1441 tcgctaattt catttccatc caaatagtct tccataaatt tttcatcttc ttcagataaa
     1501 atatccatta aatttttttg tgctttttca gcttcttttt tatatttatc cggaatttct
     1561 tcaatgcaat aattagttcc taaagagtcc agtgtatagg aataagcttt catcttaata
     1621 atatcaataa ttccttgaaa attgctttca cttccccatg gaatctgtgt aatcaatggt
     1681 atggtggcaa atttttctct gatcatttta attgttccat aataatctgc accaatgcga
     1741 tccattttgt taacaaaagc aattctaggt acttgatatc ggtcggcttg atgccaaacc
     1801 gtttcggtct gtggttctac tccagctaca gcacagaaaa ctactattgc tccatctaaa
     1861 actctgagag agcgctcaac ttcaactgtg aagtctacat gccctggtgt atcaatagta
     1921 tttatttgaa aacctttcca gaaagaggtt gtaactgcag aactaatggt aatccctctt
     1981 tctctttctt ggggcatcca atccatagtt gctgttcctt catgcacttc acccatttta
     2041 tgtactctgc cagtataata taaaatcctt tctgtaacgg ttgttttacc ggcatcaatg
     2101 tgtgccataa taccaatatt tctaacttgt tgtaaactat tggagtttaa cattgaatgt
     2161 cttttctcta tatctttttt tataaatttt tcaaaattaa tagattgttt tttttcttta
     2221 atactcataa taaaaaaaat atattaccaa cgataatgtg caaaagcttt attcgcttct
     2281 gccattttat gtgtatcctc ttttttcttt acagaagcac ctgctccttg tgaagcttca
     2341 attaattcag ctgctagctt ttctgaaaaa ggctttccag ttttacttct actgaaattt
     2401 ataatccatc tcatagctag tgtaactctt ctttcgggag aaacttcaat aggtacctgg
     2461 taacttgccc cgccaactct tctggacttc acctctaaaa ttggcattac attttccata
     2521 gcttggttaa aaactgttaa tgaatccttt tttgtctttt cttttacaat gtccattgca
     2581 tcataaaaaa tattgcgtgc aatatttttt ttaccatccc acataatatg attaataaac
     2641 ttacttactc tcttatcttg aaaaacggga tcttcattta tttcgcgttt aattgctttt
     2701 ctttttctgg acattactat ccacctctat taagctaaat tttaaaattt tttattatcc
     2761 cttattaaat atattactaa aatttctatt actttttcgg cctatcagta ccatatctag
     2821 agcggctttg tttcctaccc tcaacaccag ttgcatccat aactccacgc actatatgat
     2881 acctaactcc aggtaaatct tttaccctgc cacctctaat caatacaata gaatgctctt
     2941 gcaagctatg accaatcccg gggatatagg ctgtaacttc catattatta gtcaatctta
     3001 ctctggcaac ttttctcaaa gcagagtttg gttttttggg tgtaatagtc catactctag
     3061 tgcatacccc tcgttttaat ggggaacctt ttaaagctgg ggttccggat ttttttataa
     3121 ctttttttct tccctttttt actaactgat taatcgttgg catttttatt ccaccttttt
     3181 aaagtaattg ccagtcaaaa tacttaaaca ttttagcaac atcatttaat gatgtcaata
     3241 ataattaatt aaatatttta atatggcgat aaattttatt taattttgat tttcttcatt
     3301 aagattctct tgattattta taatattgtt ctcctgttta accacaatat tttcttctat
     3361 actctctttg atgtcttctg actctaactc tttttcgtat tgagaagtta tttcaacttc
     3421 tctatatttt ggtaaaccag tccccgctgg tatcatattt cctataatga cattttcttt
     3481 taatccacaa agataatcta cttttccctt gattgatgca tttgttaaaa cgtgtgttgt
     3541 cctttgaaaa gatgcgccgg ataaaaaact ttcggttgat aaggatgcct gagttatccc
     3601 ctgaataata ggcattccta ctgcgggcaa ctttattctt ttcataaaac caatattttc
     3661 cattaacaat atcggttctt gcaagctctg ttcggttcta tatttcacca attcaatatt
     3721 ttgttggaaa aatatattgg cattattttt tgtgattctc tcaccagcag ataatagtgt
     3781 tacaccacta ttactatcaa tgatatcctg tgcaattatt ttaccgataa gattatgata
     3841 gatatagtct aaggttcttc cttctaagga aaagaaatga ccgccttcta aaggaatatc
     3901 gctaatcccc gcagaatatg cttttctgat aatattttta ctaataagtt gtcccatgtc
     3961 tgcaataaca tttccagttt tagaatctct taattcttcc cctaattttt tccctataac
     4021 atcttctttg ataaggttta acaaattttc cttaatatta atctctttaa tatcgctcca
     4081 gactaatatt tctttacact gatgttctaa tagtttctct atatcatttt catttaaaat
     4141 ggtattggca taaactataa cattttgttc atcacggtct tttatatcct gtgctatcat
     4201 tttccctaaa acttttattt taatatctgg gttaaagatc cttattctat tggtaccgtt
     4261 tttcatcaat tcaagagcgg tttctttagt tattttactg cctattaatt tttgtaaatt
     4321 atcagattca ttatggtttg tattcaaaat atttaaggaa tttttggaaa tatcatcgat
     4381 aaaaatatgg tcctttatcc gctcaataaa atttatttcg ccctggataa tattatccag
     4441 gtttccgctt tcatccaata tatcaaagct ttgacaatca gaattttcaa tttttttaat
     4501 aatattttca tctattatgg tgtttttatc aatgatactt tcactcttac ttccggttaa
     4561 acaattaact aaagttttat tgagcagcaa ttccctgttt ttattacgaa tttcttcatt
     4621 ttcctgggct acttttttat tagcctgttc aaaatccgaa acatatacca attctcctga
     4681 aagcaaatct gaatctcgtg aagatctgat atatatttta tttaaacgtg caatctgtct
     4741 gactattacc tcaatatgct tatcattaat agtgaccccc tgggaacgat aaacttcttg
     4801 aacttgctct aaaagatact tttgaaccgc cttaataccc tgaattctca gaatatcgtt
     4861 tggatcaata aaaccag
//
LOCUS       DAOQWT010000045         4831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_17862_length_4831_cov_3.558836, whole genome shotgun sequence.
ACCESSION   DAOQWT010000045 DAOQWT010000000
VERSION     DAOQWT010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17862_length_4831_cov_3.558836"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PKJ95_01595"
     CDS             complement(<1..225)
                     /locus_tag="PKJ95_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64967.1"
                     /translation="MNDKQKLLRHFLAALAYRTQKALRGAPADFGTFQAGNEIRTPAE
                     LIRHMTSVLGYARTFFIGGRYKAEPLPSLHE"
     gene            complement(249..1679)
                     /locus_tag="PKJ95_01600"
     CDS             complement(249..1679)
                     /locus_tag="PKJ95_01600"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNR64968.1"
                     /translation="MSETDKNNNFFNKLTIVGGQPPRKNLPNVNGNIEKILLNASTDK
                     IFKEKLLNDRKSILEAPEFSLSSQDKILLQSIPDNRLKDMIENLIIQRTSRRNFLKNA
                     AATLALLATGCNAVATESSTDDAPPPLVTHKPVPEPDYSMTMGIRPEHTPIPTYIPVD
                     VGTDGAIVIHPYSNLKLIIPRGALDKKVEIIIGVIPEGLEYKHRFYSEEEFNNKNNFS
                     YIMPHELSPQNLQFKKEISICYPISELKYKELSGLYLTGNINWREFTEKAREKLSVEK
                     SKDGYAIIKTKNFGIYTLGYLLEPEEPIFSEMTLLSHGTVKMDVNYKRTKVEINSFYM
                     GKTQVTNKEYCRYNPNYLNEGNDLPAVNLSWYEAVKYCNWLTMNSPNLSSKDCCYSGV
                     PPDITCNISKKGYRIPTEAEWMYIFDKKESDKLDLTDIGDRVFQFGNGDNKTFFWCYS
                     ISHGMYGHLSPEEKNNYTGLRLAKTA"
     gene            1915..2769
                     /locus_tag="PKJ95_01605"
     CDS             1915..2769
                     /locus_tag="PKJ95_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNR64969.1"
                     /translation="MKHNERKNYSDNSGLSLLQELFDNGHYVFTTEEARNKAQTLNIS
                     DKYLLQLLHLLTHTGWITRLRRGLYALKEGFPANIQLHPFVIAVNLIKPSTISHWSAM
                     NHHGLSEQIPITITAMTTKKVVTPDMRKDSVLNNNQKHTWNISGVKYEYITVKPAFYF
                     GIEEIWVNQNFKVPVTDKERTMLEAFASPSFFGGMGEAINILEEHIIELNLERLISYA
                     LLYGKSSVSRRLGWALEHIGIPEEKIKVLAGVSACGYSILDPGRPWKGPCNSYWKIQE
                     NLSVKRNR"
     gene            2847..3443
                     /locus_tag="PKJ95_01610"
     CDS             2847..3443
                     /locus_tag="PKJ95_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64970.1"
                     /translation="MKNLIKYLKHNRYARMKELKKASFQTRDISNLLKEGIIHKIKPG
                     LYRLEEIFDVDGVSTGFLDVCQAIPQGVICLISALEFYDLTTFNPSSIYVAVPQPCKI
                     PQIIYPPIKAYYFSKNIYQAGIEHIKTRYGTVKIYNREKTICDMFRYRNKLGEDLAFE
                     GLKNYLNSRGFNIGKIIEYSKICRVEKIIMPYLKVIVA"
     gene            3443..4366
                     /locus_tag="PKJ95_01615"
     CDS             3443..4366
                     /locus_tag="PKJ95_01615"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNR64971.1"
                     /translation="MAKKKEIKNTGASVREKLLNIAKQNNLNYDSVLLQYFQERFLYR
                     VSISDYKDNFILKGGLSFLAYQIPKSRPTKDIDLLGRFISSDFETIKKIFQHIVTLQS
                     PDGIVFFPDTITVETIKKDQIYEGINVKVKGELAGARKSITIDIGFGDKLVTGPIKIS
                     FPALLDFPAPQINVYSQESVIAEKFEAIVLLQLSNSRMKDFYDIWYLASTQTFKDEDL
                     YKSIYATFNQRKTSLENKKLIFTEEFRNNPDKQSQWRAFLLKNSLYSLEQFAEVMSKI
                     ELFLEPLFMNISHQKDHMIWNNRLWKWIDKI"
     gene            4445..4744
                     /locus_tag="PKJ95_01620"
     CDS             4445..4744
                     /locus_tag="PKJ95_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64972.1"
                     /translation="MSEKDKVPDSPAIPRPGNKPYYIAPACETCKTPLVLNDLLENPD
                     TPEEEIWYDEFICPKCLDGIYMDWPESEWKNYELSQKDLEDCITLEQFLEEDRLE"
BASE COUNT         1623 a          772 c          805 g         1631 t
ORIGIN      
        1 ttcatgtaat gacggaagag gctctgcttt atatcgacct ccgatgaaaa aggtacgtgc
       61 gtatcccagc acacttgtca tatgtcgaat cagttctgca ggagtgcgta tttcattccc
      121 ggcctgaaat gttccaaagt ccgccggagc accacgcaaa gctttctgtg tacgataggc
      181 aagagctgct aaaaaatgac ggagcaattt ttgtttatcg ttcataatct tatattacct
      241 cttaactatt aagctgtttt tgcaagacgt aaacctgtat agttattttt ttcttcaggt
      301 gacaggtgtc catacatgcc atgggaaata gaataacacc aaaagaatgt tttattatca
      361 ccatttccaa attgaaatac tctatcacct atatcagtta aatctaattt atcactttct
      421 tttttatcaa agatatacat ccattctgct tctgtaggaa ttcgatagcc ttttttggat
      481 atattacatg taatatccgg aggaactcca gaatagcaac agtcttttga agataaattc
      541 gggctattca ttgtaagcca gttacagtat ttaactgctt cataccagct taaattgact
      601 gcaggtaaat catttccttc attgagataa ttgggattat agcgacaata ctctttattt
      661 gtcacctgag ttttacccat ataaaatgaa ttaatttcca cctttgtacg tttgtaatta
      721 acatccattt taactgttcc atgagaaagt aaagtcattt ctgagaaaat cggttcttca
      781 ggttctaata aatagccgag agtgtatata ccaaaatttt tagttttaat tattgcataa
      841 ccatcttttg atttttctac tgaaagcttt tctcgggctt tttctgtaaa ttctctccag
      901 tttatattac cggtcaaata cagaccagat agttctttat actttaattc cgaaatcggg
      961 taacatatac tgatttcttt tttaaactgc aaattctgcg gagataattc atgtggcata
     1021 atataagaaa aattattttt attattgaat tcttcttctg aataaaatct atgtttatat
     1081 tcaagtccct ctggaataac accaataatt atttcaactt ttttatctaa tgcacctctt
     1141 ggaatgataa gtttcaaatt ggaataagga tgaataacta ttgcaccatc agttccaaca
     1201 tctacaggta tataagttgg tattggtgta tgttcaggcc tgatacccat agtcatagaa
     1261 taatctggtt caggaactgg cttatgagtt accaatggag gtggagcgtc atctgtagat
     1321 gactctgttg caactgcatt acatcctgta gcaagtaaag ccaatgtagc agcagcattt
     1381 tttaaaaagt ttcttcttga tgtccgttga attatcaaat tctcaatcat gtctttcaat
     1441 ctattatcag gtatgctttg taatagtatt ttatcttgag atgataacga aaattctgga
     1501 gcttcaagaa tagattttct atcgtttaat aatttctctt taaaaatttt atcagtagat
     1561 gcatttaaaa gtattttttc tatattacca ttaacattcg gcagattttt tctgggtggc
     1621 tggcctccta ctatggtcag tttattaaag aaattgttat ttttatctgt ttcactcaaa
     1681 atttcccctc ttctcttata gttatagtca tcattataaa gattatataa ttatttttct
     1741 tatttttaaa gacctgtcgt gtaaaaacaa ggattatgca ttatttatta gatattctaa
     1801 agtttttaga atgataccac agcttaccat tatatcaaat tattatattt attacttcta
     1861 aaatttgact ataaataatt tatgcagtaa aatatctaaa gaggatgatt tattatgaaa
     1921 cacaatgaga ggaaaaatta ttcagataat tcagggcttt ctcttcttca agaattattt
     1981 gacaatggtc attatgtttt tactactgaa gaagccagaa ataaagctca gacacttaat
     2041 atttcagata aatatcttct tcaacttctg catcttctga cacatacagg atggattaca
     2101 cgcctgcgcc gtggattata tgcacttaaa gaaggctttc cggctaatat ccagctccat
     2161 ccttttgtaa tagcagtcaa tcttataaaa ccttcaacaa taagtcactg gtctgccatg
     2221 aatcatcatg gattatcaga acagatccca ataactatta cagcaatgac cacaaagaag
     2281 gtagtgacgc ctgatatgag aaaagactca gttctaaata ataatcagaa gcatacatgg
     2341 aatatatcag gagtaaaata tgaatatatc accgtaaagc cggcttttta tttcggaata
     2401 gaagaaatat gggttaatca gaactttaaa gttcctgtta ccgataaaga acgtacgatg
     2461 cttgaagcct ttgcatcacc ttcatttttt ggaggtatgg gagaagctat aaatatcctt
     2521 gaggaacata taattgaact taatctggag cggcttattt cttatgctct tctttatgga
     2581 aaatcatctg tttcaagacg tctcggatgg gctttagagc atattggaat accggaagaa
     2641 aaaattaaag tacttgccgg tgtatctgcc tgtggttatt ccattcttga tcctggaaga
     2701 ccatggaagg gcccatgtaa cagttactgg aaaatacagg aaaatctttc tgtaaagcgt
     2761 aataggtaga gctatttaac tattgactct ttttatctgt agttgtaata tatatacaga
     2821 taaaaagagt cagagagaag actgttatga agaaccttat taaatattta aaacataata
     2881 gatacgccag aatgaaagag ctaaaaaaag cttctttcca gacccgtgat atttctaacc
     2941 tgctcaaaga gggtattatt cataagatta agccaggtct ttaccggctg gaagaaatat
     3001 ttgatgttga tggagtcagt acaggttttt tagatgtatg tcaggcaata ccacagggag
     3061 ttatttgtct tatatctgca ctggaatttt atgatttgac tacatttaat ccttcttcca
     3121 tatatgtagc agtgcctcaa ccatgtaaaa ttcctcagat tatttatcct ccaataaagg
     3181 catattattt cagtaaaaat atctatcaag ccggcattga acatattaaa acccgttatg
     3241 gaacagtaaa aatttataac agggaaaaaa ctatctgtga tatgtttcgc tatcgtaata
     3301 aactggggga agacctggca tttgaaggat taaaaaatta tctaaattca agaggattta
     3361 atattggaaa aattatagaa tattcaaaga tctgtcgtgt agaaaaaata attatgccgt
     3421 atttgaaagt gattgtagcg taatggcaaa gaaaaaagaa ataaaaaata caggtgcatc
     3481 tgttagagaa aaattattaa atattgcaaa gcagaataat ctcaattacg attcggtttt
     3541 attgcagtat tttcaggaaa ggtttttata tagagtttcc atatcagatt ataaagataa
     3601 ttttattcta aaaggcggat tatcatttct ggcttatcaa atacctaaat ccagaccgac
     3661 aaaagatatt gacttactgg gcagatttat atccagtgac tttgaaacta ttaagaagat
     3721 ttttcaacat attgttactc ttcagagtcc ggatggtatt gtatttttcc ctgacacaat
     3781 aactgttgaa acaattaaaa aggatcagat ttatgaaggt ataaacgtaa aagtaaaagg
     3841 tgaattagcc ggagccagaa aatctatcac catagatatt ggttttggag ataaattagt
     3901 aaccggtcct attaaaataa gcttcccggc tttattagat tttcctgcac cgcaaattaa
     3961 tgtttattct caggaatcag ttatagccga gaaatttgaa gctatagtac tacttcaact
     4021 atcgaatagc cgaatgaagg atttttatga tatctggtat ttagcttcta cacagacttt
     4081 taaagatgaa gatttatata aatctattta tgcaactttt aatcaaagaa aaacgtctct
     4141 tgagaataaa aaacttatat ttacagaaga atttagaaat aacccggata aacagtcaca
     4201 atggagggct tttttactta aaaacagttt atactccctg gagcagtttg ctgaagttat
     4261 gagtaaaata gaattattcc tggaaccttt atttatgaat ataagccatc aaaaagatca
     4321 tatgatttgg aataaccggc tatggaagtg gatagataaa atttaatgac tcaaaaattt
     4381 atataaccta ttatgatttt tttcagcgaa ttatttttat ataaataaag agaaatttac
     4441 tgttatgagt gaaaaagaca aagttcctga tagcccggca attcccaggc caggtaataa
     4501 accatattat attgctcctg catgtgaaac atgtaaaaca cctcttgtat taaacgatct
     4561 ccttgaaaac cctgatactc cggaagaaga aatatggtat gatgaattta tctgtccgaa
     4621 gtgccttgac ggaatttata tggactggcc ggaaagcgaa tggaaaaatt atgagttatc
     4681 acagaaagat ttagaagatt gtataactct tgagcaattt ttagaagaag atagactgga
     4741 ataaagtgtt attttgtcta cctgtttacc tatttaattt taatatccca gtgccctcat
     4801 gttttcatcc acttcttcag tgcagtgcag g
//
LOCUS       DAOQWT010000046         4822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_17909_length_4822_cov_6.632263, whole genome shotgun sequence.
ACCESSION   DAOQWT010000046 DAOQWT010000000
VERSION     DAOQWT010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17909_length_4822_cov_6.632263"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            585..734
                     /locus_tag="PKJ95_01625"
     CDS             585..734
                     /locus_tag="PKJ95_01625"
                     /inference="COORDINATES: protein motif:HMM:NF020943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64973.1"
                     /translation="MEKGEKKYTLWLTEEQHQQIKLNATIKGVSMKEYILNLVQKDRK
                     EKHGK"
     gene            complement(806..1123)
                     /locus_tag="PKJ95_01630"
     CDS             complement(806..1123)
                     /locus_tag="PKJ95_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64974.1"
                     /translation="MKSEEKKSLTVRMSAIRLEMIKSLAKETGLSVSEFVQMKCLDLE
                     VEEEVVFIKKKKTLVEKNNDFSEYYPPPTCNRCYREVDEISEEGLCSLCEEIIKNGEE
                     LEE"
     gene            1440..2066
                     /locus_tag="PKJ95_01635"
     CDS             1440..2066
                     /locus_tag="PKJ95_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64975.1"
                     /translation="MPQRTKNLSYEELSLWEEELTLREEEVKRKECLPAICNCSDPIP
                     IIPDEMAEDLTITQKTLIENSAVNEFILKEIYVPLLEEARNDIREYRLLPPEDRKDTQ
                     IIRPSKFGVRTILEITDSIIKLLDKRDNYVTLLPDMNKEATIQSRIQEKAQEIQEGKI
                     VSFHRNALPDTVYKSAGNRPGGQQVQVLEQMKQMEEINKNQNLGQMLF"
     gene            complement(2447..>4822)
                     /locus_tag="PKJ95_01640"
     CDS             complement(2447..>4822)
                     /locus_tag="PKJ95_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HNR64976.1"
                     /translation="SIPEELKQINQWVLWKAITKTGQKKPTKIPYQLNHKEAKTNDHL
                     TWNSFQNIYEGFNGNGKNFTGIGFVFSKKDPYVGIDFDNCVDEKGNISEEVKTWIKYF
                     KSYTEYSQSKKGIHIIVKGVKPGDRKQKNGIEIYDSVRYFAFTGNTIKGYQEIRDCQK
                     ELNEFYCQIFKEEIQADTERAYEKFTPNTNKEEYYEEILSIIKENCTGMFNSYEEDFL
                     PFIMGCKAVGLPYEAIDHILQQSDGYNEKNNRKIYENLKPKTINFGRVYHYAEKATGY
                     KTKEALAEIRKKKRPRIEKPKKTSPLPKPEAAQEEGIIWGDMEVYDKPPEKNTSLNFY
                     TDTWNGEQLVKRHGENIRYCAKWKSWILWNKKRWEEDESIKIQAMAKDTIKNFHKLIG
                     KIEDEKERDQFINHIKSSESMTKRRYMVEAAMSEPGMPVMPNIFDNNKWLLNLKNGTL
                     NLESMELREHRREDYIMKMIDSNYNPKAKCETWLKFLDRIFGGNKSLINFVQRIAGYA
                     LTGLTVEQCIFILYGSGSNGKSTFINMIKEVLCGYAQQTPSETLMEKRNTGGVSNDLA
                     RLRGARFVASIETEQGKCFAEALVKQLSGGDDTITARFLNKEFFEFVPECKIFIATNH
                     KPKIKNTDHAIWRRIKLIPFTVTIPEEEKDLKFMDKLRKEKEGILAWMIEGCKLWKAE
                     GLQTPEEVIAATQEYKEEMDVIGAFIDECCVVGLKYRTLAKDLYEIYSKWCEENGEYT
                     MSQRSFGLKLTDRGYERTKVFSGYNRGKFAYVGLGILDAEPNSEPEEDYHL"
BASE COUNT         1373 a          944 c          806 g         1699 t
ORIGIN      
        1 ataaagtgat tacataacat caaaagtgat tacatgatta cattagtgta aataaaacac
       61 gaagtcacac gtagttaagc atcgaaaaaa cctagtatcc aagccatttg ttgaaggtca
      121 atccgtaaca actaatcata tcaaggggtt ctgcaagtat gggttaatat gggtaattct
      181 cctgttctga attgtttata atatcagtaa acaattaaca ggactaaaat caggaaaacc
      241 ttacagtgta taggcgaatg gcaaaatgtc gtataatcat tattatacga catttttata
      301 ttatttgtga ttaaaccatt gatatatata ggttagattg atgttatgcc ataaaaaata
      361 atagtccata cattaactaa tgaattattt tataaaaaac accggaaaag ctagcaataa
      421 cgccattaga cccaccgaaa gggcatatgg gtacgcctgg gtacggcagc cgccccagag
      481 gggagattag tatgtacata tttttcactg tttcactgtt accctattta ctataataac
      541 ctaatatgat atactacata aaattaaata aggaggaatg tttgatggaa aaaggagaaa
      601 agaaatatac tttatggtta acagaggaac agcatcagca aataaagctt aatgctacaa
      661 taaaaggtgt aagtatgaaa gagtatatat taaacttagt tcaaaaggat agaaaagaaa
      721 aacatgggaa ataattgtaa aattgtactg ctccccaaat aaaaagacag aggtataaca
      781 ttacctctgt ctcttaatga cttaattatt cttccagttc ctcgccattt ttaattatct
      841 cttcacataa actacacaat ccttcctctg atatttcatc aacttccctg taacacctat
      901 tacatgttgg aggaggatag tattcagaga aatcattgtt cttttctact aatgttttct
      961 tttttttaat aaaaacaact tcttcctcta cttctaagtc taaacatttc atctggacaa
     1021 actctgaaac acttaatcct gtctcttttg ccaaagactt aatcatttca agtctaattg
     1081 ctgacattct aaccgttaag gatttctttt cttcactttt cataataata tttcctttct
     1141 atgtaatttt atgtataata acataaataa tatttatcgt caaggcgata ctattactat
     1201 attatagttt tatattttat tttttataaa actattacat ttttatacaa acatattaca
     1261 aaataataca ataatattta taaataaata aatcatattc tatagaaatg caataatatt
     1321 tctatttctt gcaacataat ttataaaaat gattttaaat cacataaaat tgcaattaaa
     1381 ttacactttg cataaatcat tttccttatg ctataatacc cctcaaagga ggtatattta
     1441 tgccacaaag aacgaaaaac ctttcatatg aagaactctc tctctgggag gaagaactga
     1501 ccctgagaga agaagaagta aaacgaaaag aatgtctccc tgctatatgt aactgctccg
     1561 atcctattcc cattatccct gatgaaatgg cagaagatct taccatcacc cagaaaaccc
     1621 ttattgagaa ctctgccgtt aatgaattca tattaaaaga gatatatgtc ccactgttgg
     1681 aggaggcccg gaatgatata cgagaatacc gcctgctacc accggaggac aggaaggaca
     1741 cacagataat caggccgtct aaattcggag taagaacaat cctggagatt accgactcta
     1801 ttataaagct cctggataaa agagataact atgttaccct gttacccgat atgaataaag
     1861 aggcaactat acagagtcgt attcaggaga aggcccagga gattcaggag ggtaagatag
     1921 tatcttttca ccgtaacgcc ctgcctgata ctgtgtataa atcagccggg aataggccag
     1981 ggggtcagca ggttcaggtt ttggagcaga tgaagcagat ggaggagata aataaaaacc
     2041 aaaatttagg ccaaatgctt ttttaaaatt aaaaaaataa ggaaaaacct tgtatttatc
     2101 aggatttatc cttatttttt tatgtctttt tttatgttct gcacctgatg ttgcacccct
     2161 tgcacctgat gttgcacctg ataaatttac aatcaggtgc aaagaaaagg cttataccgt
     2221 aaggggtagt tatgtcttgc accccttgca cctgatattt accatgatct tgcacatgcg
     2281 cgtacgcgcg tacatgcgca tgcgcatgcg agaaaaacat gtaattatca ggtgcatcag
     2341 gtgcagaggg taaagaatgg cgttgtataa ggaaaataat tgcacctgat accccttttt
     2401 atcaggtgca tatcaggtgc agaaataagt ttatcaggtg cagagattat agatgataat
     2461 cttcttccgg ttcggaattc ggctctgcgt ctaatattcc aagaccgaca tatgcaaatt
     2521 tgcctctatt atatccagag aatacttttg ttctttcata tcctctgtct gtaagtttca
     2581 gtccgaagga tctctgactc attgtgtatt ctccgttttc ctcacaccat ttggagtata
     2641 tttcatacag gtctttagcc agggttctat attttaatcc tacgacgcag cattcatcta
     2701 tgaacgcgcc tataacatcc atttcttctt tatattcctg tgttgccgct ataacttctt
     2761 ccggtgtctg cagcccttca gctttccaga gtttacagcc ttctatcatc catgccagta
     2821 tgccttcttt ttctttcctg agtttatcca tgaatttaag atctttctct tcttccggga
     2881 tagttactgt gaaaggtata agtttaatac gtctccatat tgcatgatcc gtatttttta
     2941 ttttcggttt gtggtttgta gcaatgaaaa ttttacattc cggtacaaat tcaaagaatt
     3001 ctttattgag gaaccttgcg gttatggtat catctcctcc tgataattgt tttaccaggg
     3061 cttcagcgaa acatttacct tgctctgttt ctattgaagc aacgaacctt gctccccgga
     3121 gccgggccag atcatttgat actcctcctg tgtttctttt ttccatgaga gtttcagaag
     3181 gtgtttgctg cgcgtaacca cagagtactt cttttatcat gtttatgaaa gtagatttcc
     3241 cgttgctgcc ggaaccgtat agaataaaga tacattgctc cactgtcaac cctgtcagcg
     3301 cgtaccctgc gattctctga acgaagttta tcaggctttt atttcctccg aagatcctgt
     3361 ccaggaattt cagccatgtt tcgcattttg ctttagggtt gtaattactg tctatcattt
     3421 tcattatgta atcttctctt ctatgttccc tgagttccat agactcaagg ttaagggttc
     3481 cgttttttaa attcaggagc catttgttat tgtcgaaaat attcggcatt accggcatac
     3541 ccggctcact catggcagct tcaaccatat atcttctttt cgtcatactc tcactacttt
     3601 tgatgtgatt aatgaattga tctctttctt tttcatcttc aattttgcct ataagtttgt
     3661 ggaagttttt tattgtatct tttgccatag cctgaatttt tatactttca tcctcctccc
     3721 accttttctt attccataga atccatgatt tccattttgc gcagtatctt atattttccc
     3781 cgtgtctttt aaccagttgc tcgccgttcc atgtatcggt atagaagttt aggcttgtgt
     3841 ttttctctgg cggtttgtcg tatacctcca tatcacccca gatgatcccc tcttcctgcg
     3901 ccgcctccgg ttttggcagg ggtgatgttt tttttggttt ttctatcctg ggccttttct
     3961 tttttcttat ttctgccaga gcttcttttg ttttatatcc ggttgctttt tcggcataat
     4021 ggtataccct gccgaagtta atagttttcg gttttaagtt ctcgtagatc ttcctgttat
     4081 ttttttcgtt atatccatct gactgctgta agatatggtc tattgcttca tatggcagcc
     4141 ctacagcctt acatcccata ataaacggca ggaagtcttc ttcatagcta ttgaacatgc
     4201 ccgtgcagtt ttctttgatg atagataaga tctcttcgta gtattcttct ttgttagtat
     4261 ttggggtaaa tttctcgtaa gctctttccg tgtctgcctg aatttcttct ttgaatatct
     4321 ggcaataaaa ttcattcagt tctttctggc agtctcttat ctcctgatac cccttaatag
     4381 tattgcctgt aaaagcgaaa tatctaacac tgtcgtatat ctctatacca tttttctgtt
     4441 ttctgtctcc cggtttcaca cctttaacta tgatgtgaat acctttttta ctctgggaat
     4501 attccgtata ggatttaaaa tatttaatcc atgttttaac ttcttcggag atgtttcctt
     4561 tttcatctac acagttatca aaatcaatgc ctacataagg atctttttta gagaatacaa
     4621 atcctatgcc tgtgaagttt ttcccgtttc cattaaaccc ttcgtaaatg ttctggaagc
     4681 tgttccatgt gagatggtca tttgtttttg cttctttgtg gtttaactgg tatgggattt
     4741 ttgtcggttt tttttgccct gttttcgtta ttgccttcca tagcacccat tgattgattt
     4801 gttttaattc ttccgggata ga
//
LOCUS       DAOQWT010000047         4774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_18135_length_4774_cov_3.728120, whole genome shotgun sequence.
ACCESSION   DAOQWT010000047 DAOQWT010000000
VERSION     DAOQWT010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18135_length_4774_cov_3.728120"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PKJ95_01645"
     CDS             <1..340
                     /locus_tag="PKJ95_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNR64977.1"
                     /translation="VNTTDSAVRITHLPTGMVVTCQDEKSQHKNKAKALKILRARLLD
                     RLHQEQEEKIGQQRRKQVGTGDRSERIRTYNFPQNRVTDHRINENFHNLDDILDGNLF
                     EIIQSFKDKT"
     gene            341..1285
                     /gene="prmC"
                     /locus_tag="PKJ95_01650"
     CDS             341..1285
                     /gene="prmC"
                     /locus_tag="PKJ95_01650"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNR64978.1"
                     /translation="MSVPLLKKSTNPENEQCIGDLLSKGKLLLKRNGIENAEQESLLL
                     LSYLLKMKKIDLLLKRHFPVSSIKAKRYWQWLRQRREGYPIQYITGFQNFMGLEFAVS
                     KNVFIPRAETEILVEEVIKLIEMIPEEEPVSLMDIGAGCGVIPVAISSYFKEKGRHID
                     FHAVDLSSDALKLSFKNARRFHCQNNIHFHQGDLLKPFIKKEYFNAFHGIISNPPYIS
                     KTEWDSLSDEVRLFEPAMALLGGEKGVDFYKTIIEESPAFLKPGGFLALEIGHQQKDI
                     VSEMIRGNGIFEKKIVTFYDYYHNDRGIIAFKKNLELK"
     gene            1285..1785
                     /locus_tag="PKJ95_01655"
     CDS             1285..1785
                     /locus_tag="PKJ95_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64979.1"
                     /translation="MGKRVEGKRILFVCAGNTCRSVIAQALFQKIKEDKYPELNYYAD
                     SAGIAAYQGNSPSKEAIFCLAKQGIDITTHQAKMVDSQLLKKSSLILAMTNEQIDYLK
                     DKFPWAKNKIFLFRTFSHQKKFMKNDEIVDPFGRGIFFYEDICKYLKQDIKKLISHLR
                     EDLEYE"
     gene            1787..2245
                     /gene="rpiB"
                     /locus_tag="PKJ95_01660"
     CDS             1787..2245
                     /gene="rpiB"
                     /locus_tag="PKJ95_01660"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNR64980.1"
                     /translation="MSKIAIASDHGGFELKNEIINYLKEKLYIYKDFGCFDSQPVDYP
                     DYALKVGKAIVSNEYQLGILICGTGIGMSIAANKIKGIRAALCHDTFSARMAKEHNKS
                     NILTMGGRVIGKGLAQEIVSVWLMAEFSRESRHIVRLQKVEDIYMNPVSN"
     gene            2328..2957
                     /gene="upp"
                     /locus_tag="PKJ95_01665"
     CDS             2328..2957
                     /gene="upp"
                     /locus_tag="PKJ95_01665"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNR64981.1"
                     /translation="MKDVMVLNHPLIQHKLTLLRNKETEVKDFREIVEELSILMAYEA
                     TRDIPLKDILIETPIARTTGKIIAGPKIAIVAILRAGLGMVDGILRLLPSAEVGHIGI
                     YRDPETFKPFSYYAKLPQDIAKRDVIIADPMLATGGTAVATIDYVRRFSRTNIKFICL
                     VAAPEGIKMIRKHCKDITIYTATIDEGLNEHAYIVPGLGDAGDRLFGTK"
     gene            3129..3593
                     /locus_tag="PKJ95_01670"
     CDS             3129..3593
                     /locus_tag="PKJ95_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013755951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNR64982.1"
                     /translation="MSRPSWDEYFLQIATLVSSRSTCLRRKVGAVIVKDKQILTTGYN
                     GVPRGLAHCGEVGCLREKMGIPSGKNQELCRGLHAEQNAIIQGALHGISIQNGFMYCT
                     EQPCVTCAKMIINAGIVKIIYLNSYPDELARELLKEAKIEEIHYAQKNRKEN"
     gene            3565..4749
                     /gene="wecB"
                     /locus_tag="PKJ95_01675"
     CDS             3565..4749
                     /gene="wecB"
                     /locus_tag="PKJ95_01675"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNR64983.1"
                     /translation="MPKKTEKKIKVAFVFGTRPESIKIYPVIKEAKEYPHWIEPVIIL
                     TAQHREMLDQMIRLFQIQPDIDLGIMEHGQSLSRINSSTLLKLEKVFKDIQPDMVLVQ
                     GDTTTAFSGALAAFYQKIKIGHIEAGLRTFKKYYPFPEEINRRLITVLADYHFTPTPL
                     AVKWLVEEGVDRENIFITGNTVIDALILMQEDNFQFKDSRINRFTGPEKKILLVTIHR
                     RENWGEPLEKVCQTLIEIEQKHKNVVVFFPVHKNPIIREVVYKYLKDKENIILLEPLD
                     YREMANLMALSTIILTDSGGIQEEAPALGKPVLILRTETERPEVVNIGAARLVGTDPS
                     TICREVELLLKSPDYYNKMALQKSPYGDGLAAKRIIHYILYQYNYLAELPAEFVSPLD
                     IC"
BASE COUNT         1610 a          774 c         1001 g         1389 t
ORIGIN      
        1 tgttaatact accgactctg cagttagaat tacccacctt cctaccggaa tggtagttac
       61 ctgtcaggat gaaaaatctc agcataaaaa taaagccaaa gctttgaaaa tacttagagc
      121 acggctctta gatcgcctgc atcaggaaca ggaagaaaag atagggcaac agagaagaaa
      181 acaggtggga accggagata ggagcgagcg tatcaggact tataattttc cgcaaaaccg
      241 tgttactgac catcgtatta atgaaaattt tcataattta gatgatatat tggatggcaa
      301 cttatttgaa ataattcaaa gttttaagga caaaacttaa atgtctgtac ctttattaaa
      361 aaaatccaca aatccagaaa atgaacaatg cattggagac cttctcagta aaggtaaact
      421 attattaaaa aggaatggaa tcgaaaatgc agagcaggaa tccctcctat tgttaagcta
      481 tctcttaaaa atgaagaaga ttgatttact tctaaaaaga catttcccgg tttcttccat
      541 aaaagcaaaa cggtattggc aatggctcag gcaaagaaga gaagggtatc ctatacaata
      601 tattaccggc tttcaaaatt ttatggggct ggagtttgct gtttcaaaaa atgtttttat
      661 tccgcgggct gaaactgaga ttctggtaga ggaagttata aagttaattg agatgatacc
      721 tgaggaggaa ccggtatcct taatggatat tggtgctgga tgcggagtaa taccggttgc
      781 catttccagc tactttaaag aaaaggggag acatatcgat tttcatgcag ttgacctatc
      841 ttcggatgct cttaaactct cctttaaaaa tgcacgacgt tttcattgtc agaacaatat
      901 ccattttcac cagggagacc tcttaaaacc ttttattaaa aaggaatatt ttaatgcctt
      961 tcatggcatt atatccaacc cgccttatat aagtaaaact gaatgggata gcctctctga
     1021 tgaagtgcgc ctctttgaac cagctatggc attgctgggg ggagaaaaag gtgttgattt
     1081 ttataaaaca ataatagaag aatcccctgc atttttaaaa cctggtggct tcctggcatt
     1141 agaaattgga catcaacaaa aagatattgt ttctgagatg ataagaggta atggaatttt
     1201 tgaaaaaaaa attgttactt tttacgatta ttaccataat gacagaggta ttattgcctt
     1261 taaaaagaat ttagaactaa aataatgggg aagagagtgg aaggtaaacg tattttattt
     1321 gtctgtgctg gtaatacctg tcgtagtgta attgctcagg ctttatttca aaaaattaaa
     1381 gaagataagt atcccgaatt aaattactat gccgattcag caggaattgc tgcatatcag
     1441 ggtaattccc cgagcaaaga agccatattt tgtttagcca aacagggaat agatattacc
     1501 acccatcagg ctaaaatggt tgacagtcaa ctcctcaaaa aatcttccct tattcttgct
     1561 atgaccaatg aacagataga ctacctgaaa gacaagtttc cctgggcaaa gaataagata
     1621 tttcttttca ggactttttc tcaccaaaaa aagtttatga agaatgatga aatagttgac
     1681 ccttttggca gaggaatatt tttctatgaa gacatttgta aatatttgaa gcaggatatc
     1741 aaaaaattaa tttctcattt aagagaggat ttggaatatg agtaatatga gtaaaattgc
     1801 cattgccagt gaccatggcg gatttgaact gaagaacgaa attatcaatt atctgaagga
     1861 aaagctgtac atttataaag actttggatg ttttgattct cagccggtag attatcccga
     1921 ttatgcctta aaggtgggaa aagctattgt aagcaatgag tatcaattgg gcatcctcat
     1981 ttgtggtacc ggtattggaa tgtcaattgc tgcaaataaa attaaaggca tcagggctgc
     2041 tttatgccat gatacctttt ctgcccgtat ggccaaagaa cacaataaat ccaacatttt
     2101 aactatgggg ggaagagtaa taggcaaagg attggctcaa gaaatagtca gtgtatggct
     2161 aatggctgaa tttagcaggg aaagtcgtca tattgttaga ttacagaaag tggaagatat
     2221 atatatgaac cctgttagta actaaaagtc gattattata aagtggtaat taagaaaata
     2281 taaatgggaa tgtccctgac aggtgattgt aattaggagg atgtataatg aaagatgtga
     2341 tggtactgaa ccaccctctt attcagcaca agctgacttt acttagaaat aaagagacag
     2401 aagtgaaaga tttccgggaa attgtagagg aattatctat tctgatggct tatgaggcaa
     2461 ccagagatat tcccctgaaa gacatcctga ttgaaactcc cattgccaga actaccggta
     2521 aaataattgc cggtccaaaa attgctattg tggcaatatt gcgggctggg ctgggaatgg
     2581 ttgatggaat actgcgcctg ctgccatctg cagaggttgg gcatatcggt atataccggg
     2641 atccggaaac ttttaaaccg ttcagctatt atgccaaact gccgcaggat attgctaaaa
     2701 gggatgtaat tattgctgac cctatgctgg caacaggggg gactgctgtg gcaacaattg
     2761 attatgtgag aaggttttcc agaaccaata tcaaatttat atgtctggta gctgcacctg
     2821 agggtataaa aatgatcagg aagcattgta aagatattac catttatact gcaactattg
     2881 atgaaggatt aaatgagcat gcttatatag tgcctggttt gggagatgcc ggagaccgcc
     2941 ttttcggtac aaaataatgt agtacagtta acagtgataa gcaaaccatt taacttacag
     3001 tccatagtta atagttataa gtttatagaa agaaaataaa tatatcagag catttgcaga
     3061 tggaaagaat tttcttgtat tttactaaaa accaaaaact ataaaccaaa aacttttatg
     3121 gaggcactat gtccagacca tcatgggatg aatatttcct gcagattgcc actctggtat
     3181 ccagcaggtc cacctgttta agaagaaagg ttggagctgt aattgtcaag gataaacaaa
     3241 tattaaccac cggttataat ggtgtcccgc gtggcctggc acattgtgga gaagttggtt
     3301 gtttgcgcga aaaaatggga attccttccg gaaaaaatca ggaactgtgc agaggattgc
     3361 atgccgagca aaatgcaatt attcaaggtg cattgcatgg tatcagcatt cagaatggtt
     3421 ttatgtattg tactgaacaa ccttgtgtta cctgtgctaa aatgattatt aatgccggta
     3481 ttgttaaaat tatttattta aatagttatc ctgatgagtt agccagagaa ttattgaaag
     3541 aagcaaaaat agaagagatt cattatgccc aaaaaaacag aaaagaaaat taaagttgcc
     3601 tttgtttttg gtacaaggcc ggaatcaatt aaaatatatc cggtgattaa agaggcaaaa
     3661 gaatatcctc attggataga gcctgttatt attttgactg cccagcatag agaaatgctg
     3721 gaccagatga tacgtctttt tcaaattcag ccggatattg atctgggtat aatggaacac
     3781 gggcaatcat tatcccggat aaattcttca accttgttga aattagagaa agtctttaaa
     3841 gatattcagc ctgatatggt attggttcag ggtgatacca ccactgcctt ttccggagcc
     3901 ttagcagctt tttatcagaa gattaagatt ggccacattg aagcaggttt gaggactttt
     3961 aaaaaatatt atccctttcc ggaagaaatt aaccggcgtt taattacagt tctggcggat
     4021 tatcatttta ccccaactcc tttagctgta aaatggttag tggaggaggg tgttgaccgg
     4081 gaaaatatat ttattaccgg aaatactgtt atagatgcct taattcttat gcaggaagat
     4141 aatttccaat ttaaagattc cagaattaat agattcaccg gccctgagaa aaagatattg
     4201 ttagtcacca tacatagacg ggagaattgg ggagaaccac tggaaaaggt atgtcagaca
     4261 ttaatcgaaa tagaacaaaa acataaaaat gttgttgttt ttttccccgt tcataaaaat
     4321 cctattatca gagaggttgt ctataaatac ctcaaagata aagaaaatat aatcttactg
     4381 gagcctttag attataggga aatggctaat ttaatggctt tatcgactat tattttaacc
     4441 gattcaggtg gaattcagga agaagctccc gccttgggta aaccggtatt gatattaaga
     4501 accgaaacag agaggccgga ggtagtgaat atcggcgctg ccagattggt ggggactgac
     4561 ccatcaacca tctgtaggga agtagagctg ttattgaaga gccccgatta ttacaataaa
     4621 atggctttac aaaaatcacc ttatggagat ggtttagcag cgaaaagaat tattcactat
     4681 attctttacc aatataatta ccttgcagaa ctccctgctg aatttgtttc ccccctggat
     4741 atctgttgaa gaccagaggg aggctgtttg aatt
//
LOCUS       DAOQWT010000048         4718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_18436_length_4718_cov_2.739224, whole genome shotgun sequence.
ACCESSION   DAOQWT010000048 DAOQWT010000000
VERSION     DAOQWT010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18436_length_4718_cov_2.739224"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..533
                     /locus_tag="PKJ95_01680"
     CDS             297..533
                     /locus_tag="PKJ95_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64984.1"
                     /translation="MKNKRKLSITIDNRLWKEVDQTSRTKKYSKSRLTEEALKLWFKK
                     EREDLMAKGYKEMAEEGQRFIDITLEAQKEVLDE"
     gene            526..876
                     /locus_tag="PKJ95_01685"
     CDS             526..876
                     /locus_tag="PKJ95_01685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNR64985.1"
                     /translation="MNDIPKRGEIWLLNWNPSRGSEQAGIKPVLIIQNNVGNINAATT
                     IVAAISSSLKLYPMNVLIEPDESGLKETSIVNTSQILTVSELRLEKKLGKLSRGKMEQ
                     VDRAIKLSLALNDF"
     gene            1009..1815
                     /locus_tag="PKJ95_01690"
     CDS             1009..1815
                     /locus_tag="PKJ95_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR64986.1"
                     /translation="MCQKNRPCDIIFFIMMGIFIFNPLQSTISIITFAQENVQQTQTS
                     NQNQTIVIDIEEDETLTCMELGKELFFEQKYQDAIKQLEQCLPQEPENPEIYYYIGQS
                     YFEQGQLSAQKKNIISATKYYRQAYQISEIAIEKYLKLIEENPDSDHTNDYLRLAYIY
                     EIRSYAPGVDELQKAIDIYEKLLEEKPYLNYAHYHLGWIYYQQKDYQNAIDSFLAYLK
                     PGFKNDFVYYYLGLSYDKIGESEQAKYYFQLILDEFPDSVMASYAKKELK"
     gene            1841..2338
                     /locus_tag="PKJ95_01695"
     CDS             1841..2338
                     /locus_tag="PKJ95_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR64987.1"
                     /translation="MDRFNNKFNKLKKTVTEEYGEIVLLAYFFGSQLKGKTGPLRDYD
                     IAILLSPEQTSFQFRYKLQHELANIFNCELIDLVILNDAPIELKYHVIVAGQIIYQKD
                     SVTKVEFEADTLSRYFDYLPVLNAQRQDMLDFKPKGVKYDRRIQRYRTALRETEKKLN
                     KIRTS"
     gene            2262..2687
                     /locus_tag="PKJ95_01700"
     CDS             2262..2687
                     /locus_tag="PKJ95_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNR64988.1"
                     /translation="MTEEFRGIEQRLERLKKSLTKLEPLREKNKDEFFQDDYLQDIVE
                     RNLEVAIQSCIDIANRIISLDGLEKPKDYYGAIIILGKENILPFDFAQKFAPIAGFRN
                     ILIHEYLDIDWDEVYNNLKNIEHFYQFIDYIQKWLTQRS"
     gene            2695..2925
                     /locus_tag="PKJ95_01705"
     CDS             2695..2925
                     /locus_tag="PKJ95_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNR64989.1"
                     /translation="MDTKTYRFSIIIEKDEEGYFASCPELQGCYSQGDAYEEVLENIK
                     DAIRLHIEDRLDCGEEIPQTKYVSLTSLEVAV"
     gene            2989..4245
                     /locus_tag="PKJ95_01710"
     CDS             2989..4245
                     /locus_tag="PKJ95_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR64990.1"
                     /translation="MFSQVNNSSKKENGRNSVNSPNKVNKKTKEFKKKLPISTIPTIS
                     SKLFLSFLFLIIFALLLNGCNAFNPKNGSLEGEVHRETSYSTAPLEGALISISGSANT
                     TTTDQKGYFLLSDIPAGKRTLTIIKEGYITLKLLNVYIEPDIINEIYFGEPIILRPKE
                     DTILYDIAMDYLNQKDYQQALYTFIELRDTFPESTWADDAQYYIGNIYEISGLYISAR
                     DEYSRLLLYYPDSIWADDARFGIGNCYYQTSDYYHAKIQYQTVIDNYPLSDLVSFAQY
                     RIAWCDKRLNNNDNAIQAFQQVIMLYPQSAYAAPSQYFIAEIYYGLEDYNKAINAFQE
                     TVNNYPLAVWPGEKRLIAPAAYFYIGYCYEKKEMWQAAIDNYEIIIAQYPGSTWEDGK
                     SIVLQAQNRIDYIREHYLPPEEENSE"
     gene            4421..>4718
                     /locus_tag="PKJ95_01715"
     CDS             4421..>4718
                     /locus_tag="PKJ95_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR64991.1"
                     /translation="MEKYKNLVYKLALRMTGNYHDSEDIAQESFLRAYRSLHQYNPNY
                     SFSSWLYKITLNIIKDRMRKKDYAIPFSASHPEQDPEEVANPIFSGGDNLAINPE"
BASE COUNT         1732 a          769 c          826 g         1391 t
ORIGIN      
        1 cccaggctct ttgtttttac caaaaacctt aaacaaaaaa ccataaaccc cttccaatat
       61 tacccccatc ttataactaa aaaccaaaaa ctatgaacta aaaacctcct ccatttacac
      121 ccatatcttt tttcactatc gaccaacgac aacttccact tatccccaca tctttattca
      181 ctaaatacca acgaccactc ccacttattc ccacattctt tgtatcctct tacaaatcgt
      241 atatttgaca cgataattta tacgctatat aatactccat aggaggtact tgatatatga
      301 aaaacaaaag aaaattaagt attaccattg ataatagatt atggaaagag gtggaccaga
      361 cttccaggac taaaaaatat tctaaaagca ggttgaccga agaagccctt aaactatggt
      421 ttaagaaaga aagagaagac cttatggcta aaggttataa agagatggca gaggaagggc
      481 aaagattcat tgatataacc ttagaagccc agaaagaagt attagatgaa tgatattccg
      541 aagagaggag aaatatggct actcaattgg aatccatctc gaggtagcga acaagcaggt
      601 ataaaaccag tactaatcat tcagaataat gttggaaata ttaatgctgc aactaccatt
      661 gtggcggcaa tatctagctc tttgaagcta tatccaatga atgttttaat tgaaccggat
      721 gaaagcgggc tgaaagaaac ttccatagtt aacaccagcc agatattgac tgttagcgag
      781 ttaagactgg aaaagaagtt aggaaaactt agtaggggca aaatggaaca ggtagacaga
      841 gcgataaaat tgagtctggc attaaatgat ttttaataaa aatggggact gtgtgaactg
      901 tttgaaaatc tcaagatgtg taatataata ataaagtata tctactatat atgatatata
      961 tatgatatgg tgtataatag ttttttaata tgaaggagag gatgaaaagt gtgtcagaaa
     1021 aatcgtccct gtgacattat tttttttata atgatgggga tatttatttt taatccactc
     1081 cagagtacta tttctatcat tacttttgct caggaaaatg tacaacaaac tcaaacttct
     1141 aatcaaaacc aaacaattgt tatagatata gaagaagatg aaaccctaac ctgtatggag
     1201 ctaggcaaag aactcttctt tgaacaaaaa tatcaggatg ccataaaaca actggagcaa
     1261 tgcctgcctc aagaacctga aaaccccgaa atatattatt atattgggca gtcttatttt
     1321 gaacagggac aactatcagc tcaaaagaaa aatataatca gtgccacaaa atattaccgt
     1381 caggcatatc agatatcaga aatagcgatt gaaaaatatc taaaacttat tgaggaaaat
     1441 cccgatagtg accacaccaa tgattatctc cggcttgcct atatttacga gattcgaagt
     1501 tatgctccgg gtgttgatga actgcagaag gcaattgata tttatgaaaa attattagaa
     1561 gaaaaaccct atcttaatta tgcccattac catttaggct ggatttatta ccagcaaaaa
     1621 gattatcaga atgccattga cagtttcctg gcctatctta agccgggatt taaaaatgat
     1681 tttgtttact attatttggg gttatcctat gataaaatag gtgaaagtga gcaggcaaaa
     1741 tattatttcc agcttatcct ggatgaattt cctgactcag taatggcaag ctatgcgaaa
     1801 aaagaattaa aataattagt agaaattagt agaaaaaaag atggatagat ttaataataa
     1861 atttaataag ttaaaaaaaa cagttactga agaatacgga gaaatcgttt tattagctta
     1921 tttttttggc tctcaactaa aaggcaaaac aggtccattg agagattatg atattgctat
     1981 acttttatca cccgaacaaa catcttttca atttagatat aaattacaac acgaattagc
     2041 aaatattttt aattgtgaac tgatagattt ggttattctc aatgatgctc caatagaatt
     2101 aaaataccat gttattgtag ccgggcagat tatttatcaa aaagactcag taactaaagt
     2161 agaatttgaa gccgatacct tgagtagata ttttgactat ttacctgttc taaatgccca
     2221 aaggcaagat atgcttgatt ttaagcccaa aggagtaaaa tatgacagaa gaattcagag
     2281 gtatagaaca gcgcttagag agactgaaaa aaagcttaac aaaattagaa cctcttagag
     2341 aaaaaaacaa agatgaattt ttccaggatg attatttaca ggatattgta gagagaaatt
     2401 tagaagttgc catacaatct tgtattgata ttgctaatcg aataatttct ttagacggat
     2461 tagaaaaacc aaaagattat tatggagcaa taataatatt aggcaaagaa aatattctac
     2521 cttttgattt tgcacaaaag tttgctccaa ttgctggttt tagaaatatt ttaattcatg
     2581 aatatttaga tattgattgg gacgaagtat ataataattt aaaaaatatt gaacattttt
     2641 atcagtttat tgattatatt caaaagtggc ttactcaaag aagctaagtt taatatggat
     2701 acaaaaacat atcgtttttc tattataatt gaaaaagatg aagaggggta ttttgccagt
     2761 tgtcccgaat tacaaggatg ttacagccag ggagatgcct atgaagaagt tcttgaaaac
     2821 atcaaagatg ctattagatt acatattgag gaccgtttag attgtgggga agagatacct
     2881 caaacgaaat atgttagtct gacttcattg gaagtagcag tataatggaa aaattgccta
     2941 gaataactgc gatagttgta gtaaaaaata gtatcaagga gaagaatatt gttttctcaa
     3001 gttaataata gttcaaaaaa agaaaatgga cggaatagtg taaatagtcc taataaagtg
     3061 aataaaaaaa cgaaagaatt caaaaaaaag ttaccaatat caacaatacc tacaatttca
     3121 tcaaaactgt ttttatcctt tttattcttg ataatatttg ccctcttgct aaacggatgc
     3181 aatgccttca accccaaaaa tggttcctta gaaggagaag tgcatcgtga aacctcctac
     3241 agcactgccc cgctcgaggg tgcattgata agtatcagcg gctctgccaa caccactacc
     3301 actgaccaga agggatattt cctgctcagc gatattcccg caggcaaaag gactttaact
     3361 attattaaag aaggatatat taccttaaaa ttgctcaatg tttatattga accagatatt
     3421 attaatgaaa tttattttgg cgagccaatt attcttaggc caaaggaaga tactattctt
     3481 tatgatatag caatggatta tctgaatcag aaagattacc aacaagctct atatacattt
     3541 atagaattgc gagatacatt tcccgaaagt acctgggcag atgatgccca gtattatatt
     3601 ggtaatatat atgagataag tgggctttat atatcagcaa gagatgagta ttcacgtctt
     3661 ctcctctatt atcccgacag catctgggca gatgatgctc gttttggaat agggaactgc
     3721 tattatcaaa ccagcgatta ttatcatgcc aaaatacagt atcagacggt tattgacaat
     3781 taccccttaa gtgatttagt ctcctttgcc caataccgga ttgcctggtg tgataaaaga
     3841 ttaaataata atgataatgc aatacaagct tttcagcaag tgataatgct ttatcctcaa
     3901 agtgcctatg cagccccctc tcaatatttt attgctgaaa tatactatgg tttagaagat
     3961 tataataagg ctattaatgc atttcaggag actgtcaata attacccttt agcagtctgg
     4021 cctggtgaga agagacttat tgctcctgca gcctattttt atatcggtta ctgctatgaa
     4081 aagaaagaga tgtggcaagc ggctatcgac aattatgaga taattattgc tcaatatccg
     4141 ggtagtacct gggaagatgg caaatcaatc gttctgcaag ctcagaatcg cattgattat
     4201 attcgtgaac attaccttcc acctgaagag gaaaactcag agtaagttag ttaatacatt
     4261 aactaagccc ataacccaaa tccaaaagat taaagattaa taatgaaaac agtataaaat
     4321 gaccataaaa aatagtaatg tatttcatcc tgaccaggac aatgacctgg aaaaaatata
     4381 attatgccaa tcaggaaata atgaagcttt ttcctttttg gtggaaaaat ataaaaatct
     4441 ggtttataaa cttgctttgc gtatgaccgg aaactaccat gatagtgaag atatagcgca
     4501 ggaatctttt ttaagagcat atcgttctct gcaccaatat aacccaaact actctttttc
     4561 cagctggctg tacaagatta cccttaatat tataaaagat agaatgagaa agaaggatta
     4621 tgcgattcct ttttcagcga gccatccgga gcaggaccct gaagaggtgg caaatcccat
     4681 tttctccggt ggtgataatc tcgcaatcaa tccggagg
//
LOCUS       DAOQWT010000049         4674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_18680_length_4674_cov_2.985928, whole genome shotgun sequence.
ACCESSION   DAOQWT010000049 DAOQWT010000000
VERSION     DAOQWT010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18680_length_4674_cov_2.985928"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKJ95_01720"
     CDS             <1..390
                     /locus_tag="PKJ95_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HNR64992.1"
                     /translation="NKPIKLFVNIGGASANFGDTVNSINFPNGLVQGGLEIPSAPERG
                     LIFEFLRDGIPVIHLLNIRDLALKNDIPIDPIPLPKIGQSGVYYQYKIQKGIIIVTIL
                     VTIITLILPIYFKKLSEKKLEEINCNL"
     gene            447..1097
                     /locus_tag="PKJ95_01725"
     CDS             447..1097
                     /locus_tag="PKJ95_01725"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNR64993.1"
                     /translation="MVKDKTDRTKIILIRHGECRGNREGLFRGRSDFPLNEVGLIQAK
                     ELAVEMRSYKPLKIFTSPLERAKQTALIIGQECQTEIEVREGINNIELGPWEGQVKQF
                     IAQKYPEQWKIWLQNPEKLSVPGIETLDHVQSRARRELDMIIKDFAGSSVIIVSHRAV
                     LKPLIASCLKISKPYFWRIHIDTAAYSILHYKNDLGYTLVQLNQNKHLSEYISEWV"
     gene            1143..1334
                     /locus_tag="PKJ95_01730"
     CDS             1143..1334
                     /locus_tag="PKJ95_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR64994.1"
                     /translation="MDKINLNPIKKPLIIFLILIISSLTIPFFLPATFLGSYLFWIVL
                     TLIVIFYGLLIIAGGEKNE"
     gene            1327..2745
                     /locus_tag="PKJ95_01735"
     CDS             1327..2745
                     /locus_tag="PKJ95_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNR64995.1"
                     /translation="MSRTSVMLWGLLLWFLLGCIFSYYARKRTGPGIAEYFIANRTIG
                     GFISAMTYSATTYSAFMMVGLVGMTYSVGISAMGFELSYLMGTILLLVLFAPYFWTIA
                     KKYNCISPVELLSKAYQSRLLGAIATLFSMFMLIPYMSVQFTGIGYLMETLTGIPYRV
                     SVFITLIIILVFTLWAGMRSVAWTDCLQSIIMLVSSLMLLYYIMNHFMGGMNQFYKII
                     QSNHSELLAAGKMPYQRFLGLTIPWLFFALTNPQVAQRMFIPKDQRSLKTMIIGFATF
                     GLIYTLIVIHLGLAARHIIPVVANTDMITPLILEKVPTALALIVFLGIVAASVSTINS
                     IILTLSSMCTVDLYGQYFEVEEKSKLMFGKIMVPLISLLAFFFSLGKFGIIVELSVMS
                     SAGLLALAPSYFGIFWHRRDLTAALSSIILGGLTTVAIYFTGYYPWGIWPGVWCIIIS
                     TIVFIVISEIKYLHKTGLNKTN"
     gene            complement(2800..4359)
                     /locus_tag="PKJ95_01740"
     CDS             complement(2800..4359)
                     /locus_tag="PKJ95_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNR64996.1"
                     /translation="MSFSIFNSTTVNKTRLRTPRDFSPFSGICSVCTNDCQGTCEIGR
                     SAIRGPELLYPTERTSSQTASEKDYPVDFSHFNINGRCFGSFGTESGRTAYPRVDLDV
                     EVGNGTNKIKLKAPFVLPAMAKLNWKGYFSGAAVSGLIAVIGEDMPLTDPDVLIKEGR
                     ITHLPMLKEMYDSFYNYNEGYGALFLQANVDDEKLGLLDFAIKEIGFDAVELKLGQAA
                     KGIQGTRKLPSLEEALQVQKMGYEVLPDPSDLTIQELYKRKMCLPFYKIGRLPEWEEN
                     TFSRRIQELRQMGARFISIKTGPFRPADLARAVMLASKTGVDLITIDGSGGGTGNSPV
                     RMMNEWGYPTVYLEAILFKICEKMRERGVPLPVIVMTGGFSFEDLIFKGLALGSPYVK
                     LIGIGRAAMTAAMVGETIGNLIEKGSIPADLSQYGQSVSEIFGGFPELRARLGNKIEQ
                     IPLGALGVFNYITRINTGLQQLMALCRKYNLDSIKRDDIIPLTEEAAGVTGLTKMMEL
                     DFEEIDRIVKK"
BASE COUNT         1419 a          865 c          859 g         1531 t
ORIGIN      
        1 aataagccca ttaaactctt tgtaaatatt ggaggagcct ctgcaaattt tggagataca
       61 gttaattcta ttaatttccc caatggtctg gtgcaaggag ggctggaaat cccctctgct
      121 cccgagagag gattaatatt tgaatttttg agggatggta taccggtaat tcacctctta
      181 aatatacgag acctggcttt gaagaacgac atccctatag accctatccc tttgcctaag
      241 attggccaat ccggggtgta ctaccagtat aaaattcaaa aagggattat tatagtgaca
      301 atactagtta ccataataac cctgattctg cccatttact ttaaaaagtt gtcagaaaaa
      361 aaattagaag aaattaattg taatctttag ataatagttt tacaatattg ctaattaatt
      421 tataaattta agaggattct tcttaaatgg ttaaggataa aacagacaga accaaaataa
      481 ttttaattcg tcatggagaa tgtcggggta atagagaagg gctgtttcgg ggtagaagtg
      541 attttccttt aaatgaagtt ggactgattc aggctaagga attggcagtt gaaatgaggt
      601 catataaacc attaaagata tttaccagcc cccttgaacg tgctaaacag actgcactta
      661 tcatagggca ggagtgtcaa accgagattg aagtacgtga ggggattaat aatattgaat
      721 taggaccctg ggaagggcag gtcaaacagt ttattgccca aaaatatccg gaacagtgga
      781 aaatatggtt acagaatcca gaaaaactat cagtaccagg tatagaaact ctggaccatg
      841 ttcaaagtag agctagaaga gagctggata tgattatcaa agattttgct ggtagttcag
      901 taataattgt ttctcatcgg gctgtcttaa aacctttaat agcttcctgt ctgaaaataa
      961 gcaagcctta tttctggcgt atccatatag atactgcagc ttactccatt ttgcattaca
     1021 aaaatgattt aggttacact ttggtccaat taaaccagaa taaacatctc agtgaatata
     1081 tttctgagtg ggtataacaa aagaaagaat tatttaacat tgagggaaag aagtattgag
     1141 caatggataa aattaattta aacccaataa aaaaaccatt aataattttt ctcattctta
     1201 tcatttccag tctaacaatc ccatttttcc tgcctgccac ctttttggga agctatcttt
     1261 tctggattgt cttaacactg atagttattt tctatggatt actgataata gcaggtggag
     1321 agaaaaatga gtagaacatc tgtaatgctc tggggtttgt tattatggtt tttactgggc
     1381 tgtatattct catattatgc cagaaagaga acaggtcccg gtatagcaga atattttatt
     1441 gccaatcgta caattggtgg atttatttca gcgatgacct atagtgctac cacctatagt
     1501 gcatttatga tggtgggttt agtgggaatg acctattctg taggcataag tgcaatgggg
     1561 tttgagctgt cttatttaat gggtactata ctattgctgg tcttatttgc tccttatttc
     1621 tggacaattg ctaaaaaata taattgtatt tcacctgtcg aattactgag caaagcatat
     1681 cagagccgat tacttggtgc tattgccacc ttattctcta tgtttatgtt aataccatat
     1741 atgtctgttc aatttactgg tattggctat cttatggaaa ccttaaccgg aataccctat
     1801 cgggttagtg tttttataac tctgataata atattggttt ttactctttg ggctggaatg
     1861 cgttctgtcg cctggactga ttgtttgcaa tctattatta tgctggtctc ttctttgatg
     1921 ctcttatact acattatgaa tcacttcatg ggcgggatga atcaatttta taaaattatc
     1981 cagagcaacc atagtgagtt gttagcagcc ggcaaaatgc cctatcaaag attcctgggg
     2041 ttaaccattc cctggctttt ctttgcttta accaatcccc aggttgcaca aaggatgttt
     2101 atacctaaag accagcgaag tctcaaaaca atgattattg gctttgccac cttcggtctt
     2161 atctataccc ttattgtaat acatttagga ttggcagccc gccatattat tccagtggtt
     2221 gccaataccg atatgattac acccttaatt ttggagaaag tacccaccgc gttagccctc
     2281 atagtctttt taggtattgt ggctgcttcg gtatccacca taaattctat aattctcact
     2341 cttagttcta tgtgtacagt cgacctttat ggacagtatt ttgaagttga ggaaaagagc
     2401 aaactgatgt ttggcaaaat tatggttccc cttatttcat tgttggcttt tttcttttct
     2461 ttggggaaat ttggtattat agttgaacta tcggtaatgt cttcagctgg attattggct
     2521 ctggcaccca gttattttgg tattttctgg cacaggaggg atttaactgc agctttgagc
     2581 agtattatat tggggggatt gacaactgtc gctatctatt tcaccggtta ctacccctgg
     2641 ggtatctggc caggggtatg gtgcattatt atctctacca ttgttttcat tgtaattagt
     2701 gaaataaagt atctccacaa aactggttta aataaaacca attaaaaaaa taattcttta
     2761 tagctttgga aatgattttt ttaacggtct ctaccttttt tattttttta caattctatc
     2821 aatttcctca aaatcaagtt ccatcatttt agttaggccg gttactccgg cagcttcttc
     2881 agtaagggga ataatatcat ctcttttaat agaatccaga ttatatttcc tgcacaaagc
     2941 cattagctgc tgcaatccgg tattgattct cgttatatag ttgaacacac cgagggcacc
     3001 caaaggaatc tgctctattt tgttgcccaa tctcgccctt agctcaggaa aaccaccaaa
     3061 tatttctgat acagattggc catactggga taaatcagcc gggatagacc ctttctcaat
     3121 tagattgccg attgtctcgc caaccattgc tgcagtcata gcggctctac cgatgccgat
     3181 aagctttaca tagggggaac caagggctaa acctttaaaa ataaggtctt caaaagaaaa
     3241 accaccggtc ataactataa ccggtaaagg aacgcctctt tcccgcattt tttcacatat
     3301 tttaaagaga attgcttcaa gatagacagt tggatatccc cattcgttca tcattcgtac
     3361 aggactgttt ccagtacctc ctcctgaacc atctattgtt attaaatcaa ctccggtttt
     3421 agaggcgagc ataactgctc tggctaaatc ggcaggtctg aaagggccgg ttttaataga
     3481 aataaatctg gcacccattt gtcgtaattc ttgaattcgc ctgctaaagg tattttcttc
     3541 ccattctggt aaacgtccaa ttttatagaa gggaaggcac atctttcttt tataaagttc
     3601 ctgaatagtt agatcgctag gatcaggcaa aacctcatag cccattttct gaacttgaag
     3661 agcctcttca agagagggga gttttcttgt tccctggata cccttagctg cctgcccaag
     3721 tttcaattca actgcatcaa aaccaatctc cttaatagca aaatccaata atcctaattt
     3781 ttcgtcatct acatttgcct gtaagaatag ggcaccatat ccttcattgt aattataaaa
     3841 agaatcgtac atttccttta acataggaag atgggtaatc ctgccctctt taataagaac
     3901 atcaggatcc gttaaaggca tatcctcacc aatcactgca atcagacctg aaactgctgc
     3961 tcctgagaag tatcctttcc agtttaactt tgccatagcc ggtaacacga aaggtgcttt
     4021 taatttaatt ttatttgttc catttccaac ctctacatct aaatctaccc tgggataggc
     4081 agtcctacca gactcagttc cgaaagaacc aaaacaacgc ccatttatat taaaatgtga
     4141 aaaatctacc ggataatctt tttctgaggc ggtctgagaa gaagtccgtt ctgtgggata
     4201 taatagttca ggacctctta tagcagaacg gccaatctcg caggttccct gacaatcatt
     4261 ggtgcaaaca gaacaaattc ctgagaaagg tgaaaaatct ctgggagttc tgagtcttgt
     4321 tttgtttacc gttgttgagt taaaaatact aaatgacatt tgaaatacct cctatctaat
     4381 aaaatataca tacaaagtat cattttaaaa attatcatat ttttatttac ttactaattt
     4441 actacaatat cctgctgtag tcaaaaggag caggcctcta taaagttagt ctgataagag
     4501 tatacttaaa tttttttatt aatacttttt aattggtgga aagtaaaatg attttatgtt
     4561 aaattaaatg gcaaacaaag tatatataat aataaaaaac aaaaggttca aagtgtatgg
     4621 tttttagttt tttgagaaga ataaataccg agggctttta aaagaattag acct
//
LOCUS       DAOQWT010000050         4666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_18716_length_4666_cov_3.511603, whole genome shotgun sequence.
ACCESSION   DAOQWT010000050 DAOQWT010000000
VERSION     DAOQWT010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18716_length_4666_cov_3.511603"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="PKJ95_01745"
     CDS             <1..1680
                     /locus_tag="PKJ95_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNR64997.1"
                     /translation="LGVYLYTQYASPLNGYALSEGNPIIITTGPLTGTPVPTAGRAAM
                     TSRSPLTGTIFTSSSGGISGASLKFTGYDALVIIGKSEKPVYIYISDNEIGIEDATAL
                     WGKNTIETTQNLKEKYSGYSSIISIGPAGENQVLFASVITDGARGFGRGGLGTVWGYK
                     NIKALVIKKGRKKPRIKDKEKLKNVVYEANKSIKQNPVTSKALPELGSSFMLDVVYFD
                     RALPVKNFSDNRFPTIDRIGSTALQEEIFENSISCWGCPISCGRICHDNEDKVSEGPE
                     FETIWALGPNLGIDDLVFIQKANRLANEYGLDTISLGGVIAFAMEATERGILDFGVKF
                     GEKEKIPDLIKKIAKNNGIGQDLKTGTGNLARKIGKKASYFAINVKGLELAAYDPRII
                     KGMAVGYATSNRGGCHLHGGYSAGSEILGLPRRVDPALQISKGTLVAKRQNDSAAVDS
                     LIVCQFASMAVSLEIWSRILTAVSGKQYSAKRLSQIGERIHNLERIINLKLGFTRKDD
                     SLPPKLIEEHLGEEKIELDVMLDEYYEFRGWSREGIPTEKKVLELELNNFL"
     gene            1677..2291
                     /locus_tag="PKJ95_01750"
     CDS             1677..2291
                     /locus_tag="PKJ95_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNR64998.1"
                     /translation="MKKVSPSIVQYFQKIGWASYIAGLQDTHSGNMSMRIGNRMLITR
                     RGSMLGFLNDYDIIEISLEKNDSGVSLASSEIGIHRAIYRETNGLAIVHAHLINAVVL
                     SLINDEIVPVDVEGSYTIRKVPVVSFEYGSGSREMEEEIPRHLKDNKIMVIKGHGAIA
                     IGETMEEALFYNSVLENSCKIIVGINSLGKKADDFKPKNISEGW"
     gene            2565..3467
                     /locus_tag="PKJ95_01755"
     CDS             2565..3467
                     /locus_tag="PKJ95_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNR64999.1"
                     /translation="MTTGIIFNIKKFAIHDGPGIRTTIFFKGCPLRCQWCHNPESWFH
                     QPEIYFNQEKCIQCYQCLSSCPEQAIILKDNFPHTDKNRCTISGRCVSICPAQAREII
                     GRRVTPEEVMEEIRKDLVFYQESRGGVTFSGGEPLGQIDFLYDLLLLCQKEGIHRAVD
                     TSGYAPWSDVEYILPLVDLWLYDLKLLNEEKHKKYTGVSNQLILENLKRLSSSGVAIE
                     VRIPLIPGINDNEEELIEMVRFLDSLKIKRVSILPFHRMGLDKYTRLGLENSMKDVEA
                     ISETELEKIIKPFRKSNFIITIGG"
     gene            3470..>4666
                     /locus_tag="PKJ95_01760"
     CDS             3470..>4666
                     /locus_tag="PKJ95_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNR65000.1"
                     /translation="MNSRVARLREISLTVKPSIDIERAKLITEAYQLYQGKVPVPILR
                     ALAFKHLLEKKTVAIVQDELIVGERGSAPCATPTYPELCCHTLEDFEIIDKRDKISFQ
                     VSSEVKKIQEEMIIPFWQERSIRHQVFSLMDQNWLDCYQAGVFTEFMEQRAPGHTVAD
                     GKIYREGFLDLKKKIEKNIDSLDFHNDSGAYQKLIQLRAMSICADAIVALANRYAQLA
                     EKLAAREENLSRKKELHTISEVCRQVPAYAPRNFWEALQMYWFVHIGVISELNTWDSF
                     NPGHLDQHLLPFYEQGLSSGTLNREKAKELLECLWIKFNNQPAPPKVGVTLAESGTYT
                     DFANINSGGLTPQGEDGVNEVTYLILEVIDEMRLLQPSTNIQLSQKSPDLFLKRACEI
                     IRKGWGQ"
BASE COUNT         1480 a          791 c         1059 g         1336 t
ORIGIN      
        1 ctgggagtat atttgtatac tcagtatgcc agtccattga atggttatgc cctatcagag
       61 ggaaacccga ttattattac taccggacct ttgaccggca cgccagttcc cactgcagga
      121 agagcagcaa tgacctccag gtctcctttg accggaacaa tttttacatc cagttccggt
      181 ggtatatcag gagcatcctt aaaatttaca ggatatgatg ctctggttat tatcggtaaa
      241 tcggaaaaac cggtctatat ctatatcagt gataatgaga taggaataga agatgccact
      301 gccttatggg ggaagaatac gattgagact actcaaaatt taaaggaaaa atattccgga
      361 tattcaagta tcatctctat tggcccggct ggtgaaaatc aggtcttgtt tgccagtgtg
      421 attaccgatg gggcaagggg ttttggccga ggtggattgg ggacagtatg gggttacaag
      481 aatataaaag cactggtgat aaaaaagggc aggaaaaaac ctcggataaa agataaagaa
      541 aaattaaaaa atgtagtgta tgaagcaaat aagtcaatca aacaaaatcc ggttacctct
      601 aaagctcttc ctgaacttgg ttctagtttt atgctcgatg tggtttattt cgatagggcc
      661 ttgcctgtta aaaatttttc tgacaatagg ttcccgacaa tcgataggat aggaagtact
      721 gccttgcaag aagaaatctt tgaaaattca atttcctgtt ggggatgccc catatcttgc
      781 ggtaggatat gtcatgataa tgaagacaaa gtttcggaag gaccggagtt tgagactatc
      841 tgggctttag gaccaaattt agggatagat gatttagtat ttattcagaa agcaaatcga
      901 ttggcaaatg aatatggcct ggataccatt tcccttggtg gagtaatagc ttttgctatg
      961 gaagccacag aaagaggaat acttgatttt ggggtaaagt ttggagagaa agaaaaaatc
     1021 cccgatctta ttaagaaaat tgctaaaaat aatggcatag gccaggattt aaaaacaggt
     1081 actggaaatt tagctcgaaa aataggtaaa aaagccagct actttgccat aaatgtcaaa
     1141 ggattagaat tggcagctta cgatccgcgt attattaaag gtatggcagt aggttatgct
     1201 acttccaacc gagggggctg tcatttacac ggaggttatt ctgcgggctc agaaatatta
     1261 ggtctgcccc gtagagtcga tccggctcta cagataagca aaggaactct tgtggcaaaa
     1321 agacagaatg attcggcagc agtggatagc ctgattgtat gtcagtttgc cagtatggca
     1381 gtatctttag agatctggtc caggatattg actgctgtgt caggaaaaca atattctgca
     1441 aaaaggctga gccagatagg tgagagaatt cataatctgg agcgaataat caatctaaaa
     1501 cttgggttta ccagaaaaga tgattcttta ccacctaaat taatagagga acatttaggg
     1561 gaggaaaaga tagaattgga tgttatgtta gatgaatatt atgaattcag aggatggagc
     1621 agggagggta tcccaactga aaagaaagtt ttagaattgg agttgaataa ttttttatga
     1681 aaaaagtctc accaagtatt gtacaatatt ttcaaaaaat tggatgggct tcttatattg
     1741 ccggactgca agatacccat agtggaaaca tgagtatgcg tattggtaat cgtatgctaa
     1801 ttaccaggcg tggctccatg ctcggctttt taaatgatta tgatattatt gagataagtt
     1861 tagaaaaaaa tgacagtgga gttagtctgg cttccagtga aataggaata catagggcaa
     1921 tttataggga gacaaatgga ttggctattg tccatgccca tcttattaat gctgtagttc
     1981 tttctctgat taatgatgag attgttccgg tggatgtgga aggctcctat accatcagaa
     2041 aagttccggt ggttagcttt gagtatggca gcggttcgag agagatggaa gaagagattc
     2101 ccagacatct gaaagacaat aagattatgg tgataaaggg gcatggtgct attgctatag
     2161 gagagactat ggaagaagct ctcttttata acagtgtgtt ggaaaactcc tgtaaaataa
     2221 ttgttggaat taactctcta ggcaagaaag cggatgattt taaaccgaaa aacatttctg
     2281 aaggatggta aatatgcctt gagtggtgca tggccacgaa gaaatctcat ccaattgtac
     2341 tggaatagaa ttaaataata tggttttagc ttataatttt cagttaattg cttttcttta
     2401 aatataatag tacatttcta gattcttcta tttttctagt aaattagatg ctgactgatt
     2461 gttaattact tattataatc gttacttata actataattt ttatggttca ctgctattat
     2521 actgatatac ttatagagaa gtttttaaga ggttagtata aaaaatgacc accggaatta
     2581 tttttaacat caaaaaattt gctatacatg atggacccgg tattcgtacc accatctttt
     2641 tcaaagggtg ccctttgcgt tgtcaatggt gtcataaccc ggaaagctgg tttcatcagc
     2701 cggagattta ttttaatcaa gaaaaatgta ttcaatgtta tcaatgcctc tcctcctgtc
     2761 cagaacaggc catcatttta aaggacaatt ttccccatac agataaaaac agatgtacca
     2821 tttcgggacg ttgtgtctct atctgcccgg cgcaggctag agagataata ggccgaaggg
     2881 taacccctga agaggttatg gaggagatca gaaaagattt agttttttat caggaatctc
     2941 ggggtggtgt cactttctca gggggagaac ctttagggca gattgacttt ctatatgatt
     3001 tgctattact atgccaaaaa gagggtatac atagggcagt agatacctca ggttacgccc
     3061 cctggtctga tgtggaatac atacttcctc ttgttgacct gtggctctat gatctgaaac
     3121 tgctcaatga agaaaaacat aaaaaatata caggagtttc caatcagcta atactggaga
     3181 acttaaagag actttcttcc tcaggtgttg ctatcgaagt gagaattccc ctcatacccg
     3241 gaattaatga taatgaagag gagttgattg aaatggtcag atttcttgat tctttgaaga
     3301 taaaaagggt tagtattctt ccctttcaca gaatgggatt ggacaaatac acccggttgg
     3361 gtttggagaa tagtatgaaa gatgtggaag ccatatcaga aacagaattg gaaaaaataa
     3421 taaaaccttt caggaagtct aattttataa ttaccatagg aggctaaaga tgaatagcag
     3481 agtagcaaga ttgagagaaa taagtctcac cgtcaaaccc tctattgata tcgaaagagc
     3541 taaattgata acagaagcct atcaacttta tcagggcaag gttcctgttc caatcttgag
     3601 agctttagct tttaaacacc tattagagaa aaagacagta gcaattgttc aagatgagct
     3661 cattgtggga gaacgaggga gtgcaccctg tgccactccc acctatcctg aactatgctg
     3721 ccatacctta gaagattttg agattattga caaaagggat aaaatatctt tccaggtaag
     3781 cagtgaggta aaaaaaatac aggaagagat gattataccc ttctggcagg aacgttccat
     3841 acgtcaccag gtcttttctc taatggacca gaattggctg gattgctatc aagctggtgt
     3901 tttcaccgaa tttatggaac agagggcacc aggccatacg gttgctgatg gtaagattta
     3961 tcgggagggc tttctggacc ttaaaaagaa gattgaaaag aatattgatt ctttagactt
     4021 tcataatgat agcggggcat atcaaaaact catccagctg agggctatgt ctatctgtgc
     4081 tgatgctatc gttgccctgg ctaaccgata tgcccagctg gctgaaaaat tagctgcacg
     4141 ggaagagaat ctctcccgta aaaaagaatt acatactatt tctgaagttt gtcgacaggt
     4201 accggcttat gccccccgta atttctggga agccttacag atgtactggt ttgtccatat
     4261 aggagtcatt agtgagctta atacctggga ttccttcaat cccggtcacc tggatcagca
     4321 tcttttgccc ttctatgagc aaggtctgtc aagtggaaca ttaaatcggg aaaaagctaa
     4381 agaattatta gaatgtctct ggattaaatt taataaccaa cctgctcctc ccaaagtagg
     4441 agtaacctta gcagagagtg gcacctatac cgattttgcc aatatcaata gtgggggact
     4501 cacccctcag ggagaggatg gggtcaatga agttacctat ctcatactgg aggttataga
     4561 tgagatgcga ctcttgcaac ccagtaccaa tatacagtta agccagaaga gtcctgatct
     4621 attcctgaaa cgtgcctgtg agataatccg caaaggctgg gggcag
//
LOCUS       DAOQWT010000051         4661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_18749_length_4661_cov_3.137647, whole genome shotgun sequence.
ACCESSION   DAOQWT010000051 DAOQWT010000000
VERSION     DAOQWT010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18749_length_4661_cov_3.137647"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PKJ95_01765"
     CDS             <1..263
                     /locus_tag="PKJ95_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HNR65001.1"
                     /translation="SIIFTASLGIGVLFSTIPVFLYQGAITKIAYLIKNWITTDIINE
                     MTAVGGILIIGLSLNILEIKKIKIANLLPSLIIIIILIALKK"
     gene            355..1566
                     /locus_tag="PKJ95_01770"
     CDS             355..1566
                     /locus_tag="PKJ95_01770"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011819547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNR65002.1"
                     /translation="MIEKGKPLSLLGDLKKDEIEEKRVLVRVDFNVPLSEDLQVNDDT
                     RVRAALDTIEYLLSHNCIVILMSHLGRPKGKIVENLRMDPVALCLEKIIHRKVIKLDD
                     CIGEYVTKMVAKLVPGDIVLLENLRFHKEEENNDPVFSQQLASLADIYVDDAFGAAHR
                     AHASTVGITSYLSSYAGFLMAQEIRSLDKLIFNSDKPFISIIGGAKISSKIEILDRLT
                     EISDKVLIGGGMAYTFFAAQGLEIGKSIFEESQIKFCRELLKKAKIMGKEIVIPEDVR
                     ATYEFSEDADSFNVSIEEIDNNMIGMDIGSKTIGLFKKIISSSKKIFWNGPLGVFEMD
                     KFAKGTNEIARHIASLYGKVFSVVGGGDSIAAINKLGLNNKFSHLSTGGGASMEYVAG
                     KKLPGVEILRK"
     gene            1761..2033
                     /locus_tag="PKJ95_01775"
     CDS             1761..2033
                     /locus_tag="PKJ95_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNR65003.1"
                     /translation="MEIEEINKIKILHRLKKIEGQIRGIQTMIADNRSYADILTQVSA
                     VKSALNRINKLLLEEKVYSCFNSSENIYLYQNSLDEIIELIIKFLR"
     gene            2153..2638
                     /locus_tag="PKJ95_01780"
     CDS             2153..2638
                     /locus_tag="PKJ95_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNR65004.1"
                     /translation="MESNRKELYVCSHCAHHLLGSAVSSFSFSQYNINEILENLLNPV
                     GKYGQGKNITGLNERLKCSDCGMDYQEFTQSGKLGCRHCYEYFRERLKPLLDNLHGHS
                     KHTGKVPLNIKEKCNRMKRIKEIKDEMEESIRKEEYEKAAELRDMIIKEEKRLKKVNN
                     E"
     gene            2631..3698
                     /locus_tag="PKJ95_01785"
     CDS             2631..3698
                     /locus_tag="PKJ95_01785"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HNR65005.1"
                     /translation="MSNKEALLIRELRDSSAQWIKGDGPLNHIVVSTRIRLARNIEGI
                     PFPSKANERQLEQVLNKGEILIKKKKLFNDFKIIKIDDLTDIEVQFLAEKRLISFSLA
                     QSKYPHRAFIYKPDETISIMINEEDHFRIQCLMAGFQLEKVWEIINCFDNQIMNEIEY
                     SFNEKEGFLTCCPTNVGTGLRASVMLHLPGLSIINKLNDLMSSISKMGYAVRGFYGEG
                     TGFQGNLFQISNQVTLGLSEEEIINKLKVVINQLIVEEEKAREELILYSRQRVEDQVM
                     RAYGVLINARIISTIEAIDLLSKIRFGIGLGIFTKIGYDTINRLMLIIQSAYIQLLNC
                     KIMDENERDSTRAKLVQGLLY"
     gene            3774..>4661
                     /locus_tag="PKJ95_01790"
     CDS             3774..>4661
                     /locus_tag="PKJ95_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNR65006.1"
                     /translation="MFKRYTEKVRKVIMIAQEEAVNLNHNYIGTEHILVGLVKENDGI
                     ASKILRELGVEPERIVEEIEKIVGRGEYQEVSEITFTPRAKKVLELASQEASRLGQNY
                     IGTEHLLLGLIKEGSGVATRILTQMGLDLEGIYSRILSLLMESDSPDAPGGVIRKESK
                     VPTLDEFGRDLTELAREEKLDPVVGRNCEIQRVIQILSRRKKNNPCLIGEAGVGKTAI
                     VEGLAQKIAANDVPEILKNKRIISLDLALIVAGTKYRGEFEKRLKKIVKEVENSNDII
                     LFIDEFHTLVGAGAAEGAID"
BASE COUNT         1716 a          576 c          937 g         1432 t
ORIGIN      
        1 cttcaataat atttactgcc tctttaggca taggtgtcct tttttctaca attcctgttt
       61 ttttatacca gggagctatc actaaaattg catatttaat aaagaactgg ataacaaccg
      121 atattattaa tgaaatgact gcagtgggtg gtatacttat tattggatta tccctgaata
      181 tattagagat taaaaaaata aaaatagcaa atttattacc ttctcttata attattataa
      241 tactaattgc tctaaaaaaa taaacattga taataataac ataaaaagat tcattccaga
      301 ttatctataa tttaaccaac taatcatttt gtaaatgaaa tgtggagagt aaatatgata
      361 gagaaaggca aacctttatc cttattagga gatttaaaga aagatgaaat agaagaaaag
      421 agagtgttgg tacgagttga ttttaatgta ccattgagtg aggatttaca ggttaatgat
      481 gataccagag tcagagctgc tttagatacg atagaatact tattatcaca taattgcata
      541 gtaatattaa tgagtcacct gggtaggcct aaaggaaaga ttgtagaaaa tttgaggatg
      601 gatccagtgg ctctttgttt agaaaagatt attcacagga aagtaattaa attagatgat
      661 tgcattgggg aatatgttac aaaaatggtt gctaagctcg tcccaggaga tattgtttta
      721 ttggaaaatt tacgcttcca taaagaagaa gaaaataatg atcctgtttt ttcccaacaa
      781 ttggcttctt tggcagatat ctatgtcgat gatgctttcg gcgcagcaca cagagcacac
      841 gcctctacag ttgggattac ctcgtattta tcctcctatg ccgggttttt aatggcacag
      901 gaaattagat cgttagataa attaatattc aattctgata aaccttttat atctattatt
      961 ggtggagcaa aaataagcag caagatagaa atacttgata gattaactga aattagcgat
     1021 aaagtattaa taggtggtgg tatggcttat accttttttg ctgcacaagg attggaaatt
     1081 ggcaaatcaa ttttcgaaga aagccaaatc aagttttgta gagaattatt gaagaaagca
     1141 aaaataatgg gcaaagaaat tgtaattcca gaagatgttc gtgcaaccta tgaattttct
     1201 gaagatgcag acagctttaa tgtatccata gaagaaattg ataataatat gattggtatg
     1261 gatattggtt ctaagactat aggtttattt aaaaaaataa tcagcagttc taaaaaaata
     1321 ttttggaatg gtcctttagg tgtttttgag atggataaat ttgctaaagg aaccaatgaa
     1381 atcgcccgac atattgccag tttatatgga aaggtttttt ctgttgttgg gggtggagac
     1441 tccattgctg ccattaataa acttgggctt aataataaat tttcacattt atcaacaggt
     1501 gggggagcat caatggaata tgtagcaggc aagaaactcc ctggagttga aatactaaga
     1561 aaataaaaag aaacagtttt ttttggaact attataacaa ttgacaacat agatctattt
     1621 cttcttttaa gacaggcttt cagtctgtct ttttttgtca caattttgtc ttgtaaatca
     1681 tatcagactg ttgacaaaat gttattttga gttataatat taatacccta ggggggtata
     1741 caaaatacaa ggaaatatga atggaaattg aggaaataaa taaaataaag attttgcacc
     1801 gtctaaagaa gatagaaggg cagattaggg gaattcagac tatgattgca gataatcgat
     1861 cttatgcaga tattctgacc caagtttctg ctgtaaaatc tgccttaaat agaataaata
     1921 aattattact ggaggagaaa gtgtattcct gttttaactc ttctgaaaat atttatttat
     1981 atcagaattc attagatgaa ataattgaac ttattattaa atttcttaga taagttatct
     2041 agatttatct gcagattgta actaaaaaaa ttaaattctt tataataagg ttaaaatgat
     2101 gatttgccaa atatgtaaaa aaaatgaggc aactgtaata ataataaagg atatggagtc
     2161 aaatagaaag gaattgtatg tttgtagcca ttgtgctcac caccttttgg gaagtgcagt
     2221 ttcttctttt tcattttctc aatacaatat aaatgagata ttagaaaatt tgttaaaccc
     2281 agttggaaag tatggtcaag gtaaaaatat aactggttta aacgagagat tgaaatgttc
     2341 agattgtgga atggattacc aggaatttac acaatcaggg aaactgggct gtaggcattg
     2401 ttatgagtat tttagagaac ggttaaaacc tcttttggat aatctgcatg gtcattcaaa
     2461 gcataccggc aaagttccgc tcaatatcaa agaaaagtgc aataggatga aaaggataaa
     2521 agagattaag gatgagatgg aagagtcaat tagaaaagaa gaatatgaaa aagctgccga
     2581 attgagagat atgattatta aagaagaaaa aagattaaaa aaggtgaata atgagtaata
     2641 aagaagcctt gctaatacgt gaattaaggg attctagtgc tcaatggatt aaaggggatg
     2701 gtcctttaaa tcacatagtt gttagtacca gaatacgttt agctcgaaac atcgagggta
     2761 ttccctttcc atctaaggcc aatgagagac agttagagca ggtactgaat aaaggtgaga
     2821 ttctgataaa aaagaaaaag ctgtttaacg attttaaaat aattaaaatt gatgacttga
     2881 ccgatataga ggtgcaattt ttagctgaaa aacgattaat cagtttttct ttagcccaat
     2941 caaaatatcc ccatcgagcc tttatttata aacccgatga aacaattagc attatgatca
     3001 atgaggagga tcattttaga atacaatgtt taatggctgg tttccaatta gaaaaagttt
     3061 gggaaattat taactgcttt gataatcaaa ttatgaatga aatagaatac tcttttaatg
     3121 agaaggaagg ttttttaacc tgttgtccaa ccaatgttgg tactggcttg agagcttccg
     3181 taatgttgca tctgcccgga ttatctatta ttaataaatt gaatgattta atgtcatcaa
     3241 tttccaagat gggttatgca gtgagaggat tctacggaga aggaacaggt tttcaaggca
     3301 atttgtttca aatttccaat caggtaacac tgggtttaag cgaggaagaa ataattaata
     3361 aattaaaagt agtaataaat caattgattg tggaagaaga aaaagcacga gaagagttaa
     3421 tattgtattc cagacagaga gtagaagatc aagttatgcg ggcatacgga gttttaataa
     3481 atgccagaat catttctaca atagaagcaa tcgacttatt gtcaaaaatt agatttggga
     3541 ttggattagg catttttact aaaattggct atgatacgat taaccgattg atgttaatca
     3601 tacaatcagc atatattcaa ttgctgaatt gtaaaataat ggatgaaaat gaacgggatt
     3661 cgaccagagc caaattagtt caaggattgc tatactaaat tgagataaat acttttctta
     3721 atgaaaatta tttaagaaaa taattttaaa taaaaaattg ggaaggatat caaatgttta
     3781 aacgatatac tgaaaaagtt agaaaagtga ttatgatagc acaggaggaa gctgtgaatc
     3841 ttaatcataa ttatattggg actgagcata ttttagttgg tttggttaaa gaaaacgatg
     3901 ggattgccag caaaatctta cgtgaactgg gtgtcgaacc ggaaagaatt gtagaagaga
     3961 ttgagaaaat agtaggaaga ggagaatatc aggaagtaag tgagattacc tttactccaa
     4021 gggctaaaaa agtattagag ttagcatctc aagaagccag tagattgggt caaaactata
     4081 ttggtaccga acatttactc ttagggctaa tcaaagaagg tagcggtgtt gcaacccgaa
     4141 ttttaactca aatgggatta gatttagaag gtatttattc caggatacta agccttttaa
     4201 tggaaagtga tagtcctgat gcccctggtg gtgttattag aaaagaaagc aaagttccaa
     4261 cattggatga attcggaaga gatttaacgg aattagccag agaggaaaag ttagaccctg
     4321 tggttgggag aaattgtgaa atccaaagag taattcaaat attaagcagg agaaagaaaa
     4381 ataatccctg tttaattggt gaggctggtg ttggaaaaac agctattgta gaggggttgg
     4441 cgcaaaaaat tgccgctaat gatgttccag aaattttaaa aaataagaga ataatctcac
     4501 ttgaccttgc tttaattgtc gctggaacca aatacagggg tgagttcgaa aaacggctca
     4561 aaaaaatagt taaagaggtg gaaaattcga atgacataat tctttttatc gatgaatttc
     4621 atacgcttgt gggtgctgga gcggcagaag gcgctataga t
//
LOCUS       DAOQWT010000052         4656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_18776_length_4656_cov_3.680504, whole genome shotgun sequence.
ACCESSION   DAOQWT010000052 DAOQWT010000000
VERSION     DAOQWT010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18776_length_4656_cov_3.680504"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PKJ95_01795"
     CDS             <1..787
                     /locus_tag="PKJ95_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65007.1"
                     /translation="VYNNIAYPLQIKNFPKEEIYKRVKKILNFVKLDGFEKRLATNIS
                     GGQQQRVALARALIAEPKVILFDEPLSNLDAKLREETRKELRNFLSELKITAAYVTHD
                     QIEALALSDVIAVMNSGYIVEVGSPRQIYFNTTHQFVADFIGRANLIKAIVKSQKKDK
                     TIVDSEIGTFICQKRDFPVESKVILCIRPEFIYLKEDNYSGEGNIITGIIENLVFVGE
                     TCEGEMSIGNKRLMIKIESESSLKKGDVVNFVVSSDHCLLVSD"
     gene            801..2468
                     /locus_tag="PKJ95_01800"
     CDS             801..2468
                     /locus_tag="PKJ95_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNR65008.1"
                     /translation="MNSSSRNTVILLVIVGFLTVSPVIMLLFGSFSRGLSVSGAFTLD
                     KYIAAYTDPDLGEIINNTVIFVFGSSLLATLLALFLSYLNNRTDITWKFLFRIISITP
                     MMIPHILFSVSWVLLLNPSNGIFNLILKQLFGLKNSLFNIYSLGGMILVEGLLDMPIA
                     YLIIAPAMASFDVNLEESSRVCGANTLQTLIRITLPVLRPAILAAFILCIVRCMASFA
                     VPSVIGMPGRIYVLATHIYRTISIGFAIDYGKAAAIGMSVLVISVILIIIYRYLTAES
                     EKYVTISSRGYRPAVIELKRAKVPLFFVLGLLLFILIVLPVLVLVYTSLVPYSMIPSS
                     RALSMMNLNHWREVLKDPISILSLKNSIFLGIFGATIGIILSIFVAYTIVRIKTRTSG
                     ILESLSFLSFSFPGIVIGVGFMWFFVRTPIYATIWALLIGYIATYIPYGIRPLSSAFI
                     QIHKHLEESSRVFGAKPLTTMRRIIVPLLIPGITSGWILMATMFLRELSLSVVLARPG
                     SEVMAVQILRFASDGLWGRLSALGIIMIVISTSLVALVTFIGIKLKP"
     gene            2963..3451
                     /locus_tag="PKJ95_01805"
     CDS             2963..3451
                     /locus_tag="PKJ95_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNR65009.1"
                     /translation="MVKDGEQEKRIAAEIDDFFLQFTEILWAHISSYYKRDKKTSLNI
                     TEHFIVEFLGKESFASMSKLAKIIYVAPTTMTSIVDRLIKRGLLQRRRAQQDRRKILV
                     TLSEEGRQFYERYHQNSLEFYSIFLSALPDKGKAFIQNLQGIKGSFPYLKELFEESRK
                     NI"
     gene            3473..>4656
                     /locus_tag="PKJ95_01810"
     CDS             3473..>4656
                     /locus_tag="PKJ95_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNR65010.1"
                     /translation="MKIKAEKENQFKQYYLKSILEIERELNTSISKGLTSAQAEQLLE
                     KVGQNQLKEGKKRTIWHMLIDQFKDVLIIILLASAGVAILLEEVTDAVVIGIIVFLNA
                     ILSVLQEFRAEKSLEALKKMTVPEALVIRDGKRKKIKSTQLVPGDVVLLETGDKIPAD
                     LRLVEVTDLSVQEAILTGESEPVEKNSQQIVNMEDVLLGDRKNMAYMGTTAIAGRGRG
                     LVIATGMDTEIGHIAGMLQEQKKETTPLEKKLNEVGKNLGMIILFVVAIVVLLGYIRG
                     ISFFDMFLTGISLAVAAVPEGLPAVVTIVLALGVQQMIKKNAIIRRLPAVETLGATTV
                     ICSDKTGTLTQNEMTVQRLVLPDREIEVGGFGYQPRGEFRQGNQSIQPNDDETISLLL
                     KAA"
BASE COUNT         1451 a          738 c          942 g         1525 t
ORIGIN      
        1 tgtatataat aatattgcct atccacttca gattaagaat ttccccaaag aagagatata
       61 taaaagagta aaaaaaatcc taaattttgt aaaactggat ggttttgaaa aaagactagc
      121 aaccaatatt tctggaggac aacagcaaag agttgcttta gcaagggctt taatagcaga
      181 acccaaggtt atcctctttg atgaacccct gagtaatctg gatgctaaat tgagagaaga
      241 gacaagaaaa gagttaagaa attttctaag tgagcttaag attactgctg catatgttac
      301 acatgatcag attgaagcat tagctctttc tgatgttatt gcagtaatga attcaggata
      361 tattgttgag gttggctctc cgaggcagat ttattttaat accacccacc agtttgttgc
      421 agattttatt ggcagagcca accttattaa ggcgatagtt aaatcccaga agaaagacaa
      481 gactattgta gattctgaaa tcggtacttt tatctgccag aagagagatt tcccagtaga
      541 aagcaaggta atcctttgta tcagaccaga attcatctat ctaaaagagg ataattattc
      601 tggagaggga aatataatta ctggaattat tgaaaatctg gtttttgttg gtgaaacttg
      661 tgaaggagag atgagtattg gcaataaacg tttgatgata aaaatagaat cagaaagttc
      721 tttgaaaaag ggtgatgtgg ttaattttgt ggtaagttca gaccattgtc tgttagtttc
      781 agattgaatg gagccaaata atgaatagca gttccaggaa tacagttatt ttgcttgtta
      841 tagtcgggtt cttaactgta agtcctgtta taatgctttt gtttggaagt ttttccagag
      901 gactaagtgt ttccggggct tttactttag ataaatatat tgcagcatac actgatccgg
      961 accttggaga aattataaat aatacagtaa tttttgtatt tggttcatca ttgttagcca
     1021 ctctattggc cctgttccta tcttatttaa ataataggac tgatataaca tggaaatttt
     1081 tgttcaggat aatttccatt acccctatga tgatccccca tatactcttt tcagtcagct
     1141 gggtattatt actcaaccca tctaatggga tttttaattt gatacttaaa cagctatttg
     1201 gtttaaaaaa ttctcttttt aatatttatt ctcttggagg tatgattcta gtagagggat
     1261 tattggatat gcccattgca tatctaatta ttgctccagc tatggcttct tttgatgtaa
     1321 atttagaaga atcttccagg gtttgtggag caaatacact gcaaactcta attagaataa
     1381 ctctacctgt tttaagaccg gcaatattgg ctgccttcat actatgtatt gtccgctgta
     1441 tggcctcttt tgctgtaccc tctgtcattg gcatgcctgg acggatatat gtgttagcta
     1501 cccatattta ccggactatt tcaattggtt ttgccattga ctacggtaag gctgcggcga
     1561 ttggtatgag cgttctggta atttctgtta tcctcattat tatctataga tatttaactg
     1621 cagaaagtga aaaatatgtt actatttcca gccgtggcta ccgacctgcg gttattgaac
     1681 ttaaaagagc aaaggtgcct ttgttttttg tattgggatt acttctgttt attctaattg
     1741 ttttacccgt tctggtatta gtttatacct ctttagttcc ttattctatg attccaagtt
     1801 ccagggccct ttctatgatg aatttaaatc attggcgtga agtattaaaa gatcccattt
     1861 ctatcttatc tttgaaaaat agtatattcc tgggtatatt tggtgcaact atcgggataa
     1921 tcttgtctat ttttgtagct tatacaattg ttagaattaa aacaagaaca tcaggtattt
     1981 tagaatcttt aagtttttta tctttttctt tccctggtat tgttattggt gtaggattta
     2041 tgtggttttt tgtccgcacc cctatctatg ctactatctg ggctttactg ataggatata
     2101 ttgcgactta tataccttat ggaattcgcc ctttgagtag tgcttttatt caaatacaca
     2161 aacatctgga agaatcatca agagtattcg gagccaaacc tttaaccact atgaggagaa
     2221 taatagttcc cttacttata ccggggatta cctctggttg gattctaatg gccactatgt
     2281 tcttacgaga attatctctt tctgtggttc ttgctcggcc gggaagtgag gttatggcag
     2341 tacaaattct tcgttttgcc agtgatggac tatggggcag actatcagcc ctgggaataa
     2401 ttatgattgt aatttctacc tctcttgttg ccctggttac ttttattgga ataaaattga
     2461 aaccataaat tacaatatga ttaatatgaa aaatacttta atcctctttt taatattaca
     2521 caactattga cccataatga ggttgtgtaa attcaaaatc atacatttta aataatgtaa
     2581 attttatgta ctttgaatag taatttaaat cgcttatgga aacaaaaaat ggaactcgat
     2641 agaacagctg ctataaccat tgaactgata gtatataatg gtcctttctt tgttaatatt
     2701 attagtctct atgaaagctt tgctccatta atcttaggaa ggattgaaat gccattcact
     2761 aatactcgga aaaccattca taatgctcat atacgaaata taatagttct tttctcagta
     2821 taaccttata tagttttaaa ggtaggataa cacaaagttt tataaaattt tattaatcca
     2881 tttgacttta ttattggtgg tcattaaaat atttatgata cgataatcat ataataaaat
     2941 atattaaaac taaggtatgc aaatggtaaa ggatggggaa caggagaaaa ggatagctgc
     3001 tgaaatagat gacttttttc tccaatttac tgagatattg tgggcacata tttccagtta
     3061 ttataagagg gataagaaaa catctctgaa tataaccgag cattttattg tagagttttt
     3121 aggtaaagag tcatttgcct ccatgagcaa acttgctaaa ataatctatg tcgctccaac
     3181 cactatgacc agtatagttg atcgcttgat taaacgtggt ttactgcaaa ggcgtcgtgc
     3241 tcaacaggac cgaagaaaaa tactggttac cctttcagag gaaggaaggc aattctatga
     3301 gagataccat cagaattccc tggaatttta ttcaattttt ttatccgcat tacctgataa
     3361 gggtaaagct tttatccaaa acctgcaggg aataaagggg agtttccctt atcttaaaga
     3421 gctttttgag gaaagtagaa aaaatatcta attagagtgg aattaattga atatgaaaat
     3481 aaaggcagaa aaagagaatc aatttaaaca atactattta aaatcaatac tggaaataga
     3541 acgagagttg aatacttcaa tatccaaagg attgacttct gctcaagccg aacagttact
     3601 ggaaaaagtt ggacagaacc aattaaagga aggtaaaaaa agaactatct ggcatatgct
     3661 gatagatcag tttaaggatg tcctgataat tatattgctt gcttcagctg gcgtggcaat
     3721 attgttagaa gaggtgaccg atgccgttgt tataggaatt attgttttct tgaatgccat
     3781 tttaagtgtc ttacaggaat ttagggcaga aaaatcctta gaagccttaa aaaaaatgac
     3841 tgttcctgaa gcgctggtta ttagggatgg caaacgaaag aaaataaagt caactcagct
     3901 ggttcccgga gatgtagttc tgttggagac aggtgataag atacccgctg atttacgtct
     3961 cgttgaggta accgatttaa gtgttcagga agcaatatta accggggagt cagaaccggt
     4021 agagaaaaat agccagcaaa ttgtaaacat ggaagatgta ctattaggag atcgcaagaa
     4081 tatggcttat atggggacta cagcaatagc cggacgaggc agagggctgg tgattgctac
     4141 cggtatggac acggaaattg gtcatattgc cggtatgctg caggagcaga aaaaggaaac
     4201 cactccatta gaaaaaaaac taaatgaggt gggtaaaaat ctgggaatga ttatcctgtt
     4261 tgtagtagca attgttgttc tcttgggata tataagagga atttctttct ttgatatgtt
     4321 tcttaccggg atttctctgg ctgtggcagc agtgccggaa gggttgcctg ctgtagttac
     4381 tattgtcctg gcacttggtg tacagcagat gattaaaaaa aatgctatta tcagacgtct
     4441 gccggcagtg gagactctgg gggcaactac ggtaatctgt tcggacaaga ccggcacttt
     4501 aacccaaaac gaaatgactg ttcagaggct ggttttgccg gaccgggaga tagaagtagg
     4561 tgggtttggc tatcagcccc ggggggaatt ccgtcagggg aaccaatcta ttcaacccaa
     4621 tgatgatgag accatctccc tcttattaaa agcggc
//
LOCUS       DAOQWT010000053         4650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_18813_length_4650_cov_3.356257, whole genome shotgun sequence.
ACCESSION   DAOQWT010000053 DAOQWT010000000
VERSION     DAOQWT010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18813_length_4650_cov_3.356257"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..573
                     /locus_tag="PKJ95_01815"
     CDS             394..573
                     /locus_tag="PKJ95_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoisovalerate dehydrogenase E1 subunit beta"
                     /protein_id="HNR65011.1"
                     /translation="MATREIIFMVEEDPEQGYTAKALGFSIFTEANTLTELKEKIKDA
                     IKCHFEQHPHVFTSV"
     gene            706..1038
                     /locus_tag="PKJ95_01820"
     CDS             706..1038
                     /locus_tag="PKJ95_01820"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNR65012.1"
                     /translation="MKKNDKNIFIVVTHIIKKENNQYVAICPEFDVSSFGDTVEEANE
                     NLKEAITLYLEGIDELKIRDQVFKEKSIKTYTGRPKSVEMNKYKFEKGFDNQPFIKKE
                     PLQVALCK"
     gene            1577..1798
                     /locus_tag="PKJ95_01825"
     CDS             1577..1798
                     /locus_tag="PKJ95_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65013.1"
                     /translation="MDMNKKVNVTFRMEQELKEVIEYIANEERRSFSNQLNVALEEWL
                     KIKNEIHPRFLEDIRDSLESGQPEPVWKG"
     gene            1808..2101
                     /locus_tag="PKJ95_01830"
     CDS             1808..2101
                     /locus_tag="PKJ95_01830"
                     /inference="COORDINATES: protein motif:HMM:NF027117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNR65014.1"
                     /translation="MDCQYKQSAYPKYNRIKKKFPLLLRKKLNDIEDSIANNPLIGEE
                     KKIDIKGIRVHKFRLLDQQILIAYQVNEDKKEVIFVAVGGHENFYRDLKNYLK"
     gene            2378..2453
                     /locus_tag="PKJ95_01835"
     tRNA            2378..2453
                     /locus_tag="PKJ95_01835"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2411..2413,aa:Ala,seq:cgc)
     gene            2608..4575
                     /locus_tag="PKJ95_01840"
     CDS             2608..4575
                     /locus_tag="PKJ95_01840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNR65015.1"
                     /translation="MKKKKSQYRNKKILLKNISIFIISLLFCLSFSLTFAVNASSSKL
                     TQRELNYIANRGPVKAISIDGAAPFFYKNTAGEIKGISVNILEEIASMTGLTLHYQLY
                     DTAVEMLKADADMAMSLSKEYQMPNFALSLPYLHTETILYYNSSLNPNELDDKRYAMI
                     IGGTLPVGIKEENAVYFNNREETFDAVNEGRADYGYANNYSLAFYMLQNEYNNIVTVP
                     YGKESRAYCFGIVEGDENLLSIINKSLSAISESRMQTLILSAVSDVKKKLTFQKVLDT
                     YWIEIFSLIALVIAVLSYAIHAKKNANKLLNLEIIKVKEQEEKIRQLSFHDSLTGLYN
                     RRYLEEELSRLDVERQLPISVIVADVNGLKVTNDALGHSEGDKLLQKAAEVLKNACRE
                     EDIAARTGGDEFILFFPQTTEEETIQIAQRIHQHCSEKCKNIIPGTLSLGYATKNNTG
                     EDISDVIKKAEEMMYSHKLLESQSIRSRIISSLQSTLHEKDIETIEHSQRIAETSMKV
                     GEKLGLAQQELDNLSLLASLHDIGKITVDESILKKPDKLTPRERKEMEKHSEAGYRIA
                     KSTNMLTEIAEYILYHHERWDGKGYPHGLKGEEIPLLSRILAIADAYDAMINDRPYQK
                     AISKEEVIEELKKESGKQFDPELVPIFISIL"
BASE COUNT         1639 a          828 c          866 g         1317 t
ORIGIN      
        1 ccgaacggca cgtacggtgg tgtgagaggg ggaagagatt tccccctact cgattcccca
       61 gatcttttca gaaattgatc ctttaacctt atcttttaat ttgtatttgt catttttctt
      121 tcttttacca aaatccaaaa actataaacc atcaacctcc cacatttatt ttcacatatc
      181 ttctctaaat accaaatacc aacgaccatt tccatttatc cccgcaatga ttcaactaaa
      241 tgcagtgaaa acagggggga cggttttctg ttttacaagt tcttgggagt caaacagaga
      301 accgtcccct gtttgaatat tcgaatgctg aaaaatgaac cataaagtgg tataattcaa
      361 tcaatactct atcatttaca ccggaggtaa accatggcca caagagaaat aatatttatg
      421 gtagaagaag accctgaaca gggatataca gccaaagccc ttgggttctc catttttacc
      481 gaagccaaca ccttgacaga attaaaagag aaaattaaag atgctataaa atgccatttc
      541 gagcagcatc cccatgtttt cactagtgtt taataaagga atactgttat tacctgctta
      601 ggtcgacatt gacaaatatt gataaatatt ctattattta gctacctcag gaggattctt
      661 tagctttatt ttacctgcta tttaaaagct aaagggggaa gaacaatgaa aaaaaatgat
      721 aaaaacatat ttattgtagt tactcatatt attaaaaaag aaaataacca atatgtcgct
      781 atatgcccgg agtttgatgt atcttctttt ggggatacag tagaagaggc taatgaaaat
      841 ttaaaagaag ctattacgct ctatttagag ggcattgatg agttaaaaat cagagaccag
      901 gtctttaaag aaaaatcgat taagacatat actggcagac caaagtctgt tgaaatgaat
      961 aaatataaat ttgaaaaggg ctttgataac cagcctttta tcaaaaagga gcctctgcaa
     1021 gtcgcattgt gtaaataatg aattatgtcg aatataccct ctgtaaccgg taaagagcta
     1081 ataaacaaat tatgtaatgc cggttatatt atattaatta agaaaaaagt tgccactttt
     1141 tcctgacatt gcctggccat tccaaaaatt ctactgttgt tcaaaataca cataaagata
     1201 ttcctatagg taccctttca accatacaga aacaattaag attaacaaga gaagaattta
     1261 ttgaacttat atcgtaaagc atggacgttc aaatatgggg acggttcacc gcttgactct
     1321 tgaggataat gtaaagcaga aagccgtccc ccagttttca ctaatgccgt cccccgtaat
     1381 tgcgttcctc catcttttcc gtcattgcga acctgccttc ggcaggtgtg gcaatctcat
     1441 cctctgcagc tcgcactctg ttcttcctct ttcactacaa ggcaagcata catatgtaaa
     1501 atacttgcat ttacatatat tgtatgttag tatttgtaaa aacaagtttg aatataatat
     1561 taaagaaggt atttatatgg atatgaataa aaaggtaaat gtaactttta gaatggaaca
     1621 agaactgaaa gaagttattg agtatattgc aaatgaagag aggcgcagtt ttagcaatca
     1681 gctcaatgtt gcattagagg aatggctcaa aattaaaaat gaaattcatc cccgattctt
     1741 agaagatatc cgggattcat tggaatccgg ccaacctgaa ccagtctgga aaggataatt
     1801 tcttctattg gattgtcaat ataaacaaag cgcctatccg aaatataatc gtattaaaaa
     1861 gaaatttccc ctccttctca ggaaaaagtt aaacgacata gaagacagca ttgcaaacaa
     1921 tcctctgata ggagaagaaa agaagattga tataaaagga atccgtgtac ataagtttag
     1981 actacttgac caacaaatat taatagctta tcaagttaat gaagataaga aagaagtaat
     2041 ttttgtagct gttggcggac acgaaaattt ttatcgggac ttaaaaaatt atttaaaata
     2101 gaatgtataa ggacagacct ttgacataat tgcaaccacc tttttaacca ctctttagga
     2161 aaagaactac tctaaaaacc tgctactaac ttatttatgg attacttatt gttatttgct
     2221 ttagaagacc tgtaccattt cggtatgaaa tgaccaaaaa cagctataga aataagctct
     2281 attttatagt agaatattaa agcattccca aagcagataa tatataatat atgtaatatc
     2341 tataaaatat aaatacaatt gataaaaaag atggtgtggg gatatagctc agttgggaga
     2401 gcgctgcgtt cgcatcgcag aggccagggg ttcgaatccc cttatctcca ccaacatgtg
     2461 aaaacatggg tacggtttca aacaggggga cggttcacag cttgactcct gaggatattg
     2521 tcaagcagaa aaccgtcccc cagttttcac taatgccgtc tcatgtttga atgaccaact
     2581 atcccaatat ttaaaccagg ctttataatg aaaaagaaaa aatcacaata tcgcaacaaa
     2641 aaaatactac ttaaaaatat atcaatcttt attatctctc tattattttg cttatccttt
     2701 tccctgactt ttgcggtcaa tgcttcttcc tcaaagctta cccagcgaga gctaaattat
     2761 attgccaaca gaggccctgt aaaggctatt tccattgatg gggcagcccc tttcttttac
     2821 aagaacactg ctggcgaaat caaaggaatc tcagtcaaca tattagagga gattgcctct
     2881 atgaccggac tcactcttca ctatcaactg tatgatactg cagtggagat gctgaaagct
     2941 gatgccgata tggctatgtc attaagcaag gaatatcaga tgcctaattt tgcattatca
     3001 ttaccctatt tgcataccga aactatactt tattacaatt cttctttaaa ccccaatgaa
     3061 ttggatgaca aaagatatgc tatgataatc ggtggtactt tgccagttgg gataaaagag
     3121 gagaatgcag tctattttaa caacagagaa gagacttttg atgcggtaaa tgaaggtaga
     3181 gcagattatg gttatgccaa taactattca ctcgctttct atatgctaca gaatgaatac
     3241 aataatatag taacggtacc ctatggtaag gaatctagag catactgttt tggtatagta
     3301 gaaggtgacg agaacctgct cagtataata aataagtctt taagtgctat cagcgaaagc
     3361 cgtatgcaga ctcttattct ctctgccgta tctgatgtta agaaaaagtt gaccttccaa
     3421 aaggttttag atacctattg gatagaaatt ttcagcctta ttgcactggt aattgcagta
     3481 ttgtcttatg ctattcatgc caagaaaaat gccaataaac tgctcaattt ggagattata
     3541 aaagttaaag aacaggaaga aaaaataagg caattgagtt ttcatgatag tttaaccggt
     3601 ttgtacaaca ggagatatct cgaagaagaa ttatcaagat tggatgtgga aaggcaactt
     3661 cccatctctg ttattgtggc agatgtgaat gggctcaaag taaccaatga tgccctggga
     3721 catagtgagg gggacaaact gttgcagaaa gctgcagaag tattgaaaaa tgcctgtcgg
     3781 gaagaagata tagctgccag aacaggtggg gatgaattta tattattttt cccacagact
     3841 actgaggagg aaactataca aatagcacaa agaattcacc aacattgttc agaaaagtgt
     3901 aaaaatataa ttcctggcac tctatcactc gggtatgcta ccaaaaataa tacaggtgaa
     3961 gatatatcag atgtaataaa gaaagccgaa gaaatgatgt atagccacaa acttttggaa
     4021 agccagagca taagaagtcg aatcatctct tctttacagt caaccctgca tgaaaaagat
     4081 attgagacaa tagagcattc acaacgcata gcggaaacct ctatgaaggt tggggaaaag
     4141 ctgggactag cgcaacagga attggataat ctaagcttat tagccagttt acacgatatc
     4201 ggtaagatta cagtggacga gagtattctt aaaaaaccgg ataaacttac ccctagagaa
     4261 agaaaagaga tggagaaaca ttctgaagca ggttatagaa tagcaaaatc taccaatatg
     4321 ttaactgaaa ttgcagaata tatactctat caccatgaac gttgggatgg caaaggctac
     4381 cctcatggcc tgaaaggaga agagatacct ttactttcca ggatactggc tattgccgat
     4441 gcctatgatg ctatgatcaa tgaccgaccc taccaaaaag caataagcaa agaggaagtc
     4501 atagaagaac tgaagaagga gagtggaaaa caattcgacc ccgagttagt gcccatcttc
     4561 atatccatcc tttagaaaaa tttgggtaca aatgtaagtg gtcgttagtc gtttgtatat
     4621 agtatttagt aaacaaacaa acagggggac
//
LOCUS       DAOQWT010000054         4626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_18951_length_4626_cov_3.118574, whole genome shotgun sequence.
ACCESSION   DAOQWT010000054 DAOQWT010000000
VERSION     DAOQWT010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18951_length_4626_cov_3.118574"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PKJ95_01845"
     CDS             complement(<1..486)
                     /locus_tag="PKJ95_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNR65016.1"
                     /translation="MNFWDDKRVIVTGGAGFLGSYVVEKLKARGCRNIFIPRAEEYDL
                     TKEQNVLRLYQGFSADIVIHLAAVVGGIGANRENPGKYFYDNLVMGAMLMEYARQFEV
                     DKFVAIGTICSYPKYTPVPFKEENLWNGYPEETNAPYGLAKKMLLVQSQAYREQYGFN
                     SI"
     gene            complement(517..1569)
                     /gene="gmd"
                     /locus_tag="PKJ95_01850"
     CDS             complement(517..1569)
                     /gene="gmd"
                     /locus_tag="PKJ95_01850"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNR65017.1"
                     /translation="MKRALITGITGQDGSYLAEFLLYKDYEVHGIIRRASTFNTGRID
                     HIYKDPHKSGVRLLLHYGDLSDSSELTNLIYNIKPDEIYHLGAQSHVRVSFDMPEYTG
                     DITGLGTTRILEAIRRSGVKCKFYQASSSEMYGDAPAPQNEDTPFRARSPYAAAKIYS
                     YWMVRNYREGYKLFACNGILFNHESPRRGETFVTRKITRGLARILAGKDKKLYLGNLE
                     ARRDWGYAPDYVEAMWMMLQQDKPDDYVIGTGETHSVKDFLEEVFKYVGLDWKEYVEI
                     DPRYFRPTEVELLLADASKARRVLGWEPRISFKELVKIMVDSDLELTGLDSPGEGKKI
                     LKEKGINWTENNFTVG"
     gene            complement(1609..2718)
                     /locus_tag="PKJ95_01855"
     CDS             complement(1609..2718)
                     /locus_tag="PKJ95_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNR65018.1"
                     /translation="MKNNILLDNIIFCLQEIGGISNYWFNLLNKFVKEDSIFFYMDSK
                     AEKNVYGKLLTLPENRCIDDKSKIPLRIKRYLPVNKIKKIYGFDIFHSSYYRYSQDDV
                     KNITTVHDFMYEKFLSKYNVRRVIHNRQKYFALKNSVYIIGISKNTLDDMMKYFPEFK
                     GKKKQVVWHGVSNDFYVVNDKKPFLKIQNQKIYYNSYYFYLGNRQGYKNFIFLLRAYA
                     RLIKNVPDCPKLLVAGGGNFTDIENRIINELGINETVIKLNNVNQEELNYIYNHCTGF
                     VYPSLYEGFGMPVLEAMKAGCPVICSNTSSLPEVAGEAAIYFNPNNVEDLEKSLYNLL
                     DLNTRKELIKKGLENVKKFTWENTCKKTLEVYDSV"
     gene            complement(2926..3759)
                     /locus_tag="PKJ95_01860"
     CDS             complement(2926..3759)
                     /locus_tag="PKJ95_01860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014548570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR65019.1"
                     /translation="MDKKVQNEAISDNLLPLVSIITVCYNSEKFITDTIESVLNQTYK
                     KYEYIIVDGGSTDNTLKIIQNYKEHISILISEPDKGVYDAMNKGAGLAKGEWTIFLNS
                     GDRFYKSNTLENIFYQKNYFVDFIYGDCKVTYNSGFSRIAKARKIEELWKGMVFSHQS
                     LFTNIEVFKEYKFNIDNKIVGDFEFIYRCYLKGYKFYYLNFPVAEILAGGLSDINRIG
                     SILSRWKTVKKYSNESVIINIYYLSLLMENFVKSLLKSILPKRVYNILLERKYSIKSE
                     K"
     gene            complement(3840..>4626)
                     /locus_tag="PKJ95_01865"
     CDS             complement(3840..>4626)
                     /locus_tag="PKJ95_01865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR65020.1"
                     /translation="NGGVSSARNSGLDVASGDYIGFVDSDDYIHKDMYRLLIEATLKN
                     DSDIIECGYNLVNEQYMVILSFPLKNNIINGNYDCSKDFLQGKNATHSNANKLYRKTL
                     FKSIRFPIYRFGEDYWVNAIAAYLIKRKATIEGCYYYYVKHKRSALNSPFSDDSLDII
                     LSGKDVYDFYRTRFEDLSSYAALYIVEHILRLYKEIKASLPKEKQKKYFKKLIYEFKN
                     YYSLIDGKVYRDVGFKKRHVALLLFKIYPELYCLLSKIYQKIK"
BASE COUNT         1490 a          900 c          566 g         1670 t
ORIGIN      
        1 aatagaatta aatccatatt gttctcgata tgcctgagat tggactaaca acatcttttt
       61 ggccaatccg tagggtgcat tggtctcttc aggatatccg ttccataaat tctcttcttt
      121 aaaaggaacg ggggtatatt ttggataaga acaaatggtt ccgatagcaa caaatttatc
      181 aacctcaaac tgtcgggcat actccataag catagctccc attaccagat tatcgtagaa
      241 atactttccg ggattctctc tattagcacc gataccaccc actacagctg ctaaatgtat
      301 aacaatatct gctgaaaaac cctgatataa tctgagtaca ttttgttctt tagttaaatc
      361 atactcctct gcccggggta taaatatatt cctacatcct cttgctttta atttttccac
      421 cacataggag cctaaaaatc cggctccccc tgttactatt actctcttat catcccaaaa
      481 attcattcta tacctctaac aatagtaaaa ttagccctac ccaaccgtaa aattattctc
      541 tgtccagtta ataccctttt cttttaaaat cttcttacct tcaccaggtg aatccaatcc
      601 tgtcaattct aaatcactat caaccattat ctttactaat tctttaaaag aaattctggg
      661 ctcccatcct aacactctcc ttgccttaga ggcatcagct aataatagct ctacttcagt
      721 tggtctaaaa tatctcggat ctatctctac atattccttc caatccaatc caacatattt
      781 aaatacttct tctaaaaaat ctttcacaga atgagtctca ccggttccaa ttacataatc
      841 atctggctta tcctgttgca acatcatcca cattgcctcc acataatcag gagcatagcc
      901 ccagtctctc ctggcttcca aattacccag atagagcttc ttatccttcc cggcaagaat
      961 cctggccaat cctctggtaa tctttcgggt aacaaaggtc tcccctctcc ggggactctc
     1021 atggttaaaa aggataccgt tacaggcaaa taatttatat ccttcccggt aattacgtac
     1081 catccaataa gaataaattt ttgctgccgc ataagggctt ctcgccctga agggagtatc
     1141 ttcgttttgg ggagcaggag cgtctccata catctcacta ctggatgcct ggtaaaactt
     1201 gcatttcacc ccgcttctcc ttattgcttc taaaatccgg gtggttccca aaccggtaat
     1261 atccccggta tactccggca tatcaaaact cacccgcaca tgactctgtg ctcctaagtg
     1321 atatatttca tctggcttaa tattataaat aagattggta agttctgaag agtcacttaa
     1381 atctccataa tggagcaaca atctgactcc acttttatgt gggtctttat agatatggtc
     1441 tattctaccg gtattaaaag tactggcacg tctgataatg ccatgcacct cataatcttt
     1501 ataaagtaga aattcagcta gatagcttcc atcctgtccg gtaatcccgg taataagtgc
     1561 ccttttcata atccttctcc cttcaatttt tgttgttcac tcagattttt acacagaatc
     1621 atatacttct aatgtttttt tacaggtatt ttcccaggta aatttcttca cattttctaa
     1681 tcctttttta attaactctt ttctggtatt aagatctaac aaattgtata aagatttttc
     1741 taaatcttca acattattgg gattaaagta gattgctgct tctccagcaa cttcaggtaa
     1801 gcttgatgtg ttagaacata ttacgggaca ccctgctttc attgcttcca gtaccggcat
     1861 tccaaatccc tcatataggg agggataaac aaatcccgtg caatgattgt aaatgtaatt
     1921 taattcttct tgatttacat tgtttaactt tataacggtt tcgtttatac ccaattcatt
     1981 aattattcta ttctcaatat ccgtaaaatt acctccaccg gctactaata attttgggca
     2041 atcgggaaca ttttttatta atcttgcata tgcccttagc aaaaaaataa aatttttgta
     2101 accctgcctg tttcctaaat aaaaataata tgaattataa taaatttttt gattttgtat
     2161 ttttaaaaag ggttttttat catttacaac ataaaaatca ttgcttacac catgccagac
     2221 aacctgcttt tttttaccct taaactcagg aaaatatttc atcatatcat ccaaagtatt
     2281 ttttgaaatc ccgattatat aaacagagtt ttttaaggca aaatatttct gccggttatg
     2341 tataaccctt cttacattat attttgataa aaacttttca tacataaaat catggactgt
     2401 agtaatattt tttacatcat cctgggagta acgataataa ctggagtgaa agatatcaaa
     2461 accatatatt ttctttattt tattaactgg taaatatctt ttaattctaa gaggtatctt
     2521 acttttatca tcaatacaac gattttcggg tagtgttaac aattttccat agacattttt
     2581 ctctgctttg ctatccatat aaaaaaaaat gctatcttct ttcacaaatt tgtttaatag
     2641 attgaaccag taattactta ttcctccgat ttcctgcaaa caaaatatta tattatctaa
     2701 taaaatatta tttttcattt attaatttta tccaattcct ttattaaagg tgcagctaac
     2761 ctttgctgct ctacctttta aaaaactatg gatttactgt taatatcttc ttccttgtga
     2821 taaatagcaa cctttgttga aaaagaataa ggttatcctt ctaagtaata agaatattca
     2881 agaaataatt taggaaagga aagcgtagaa aaattatatt tagatttatt tttcactttt
     2941 aatactatat tttcgctcta ataaaatatt gtatactctt ttcggtaaaa tactctttaa
     3001 aagagattta acaaaattct ccattagtaa agataaataa taaatattga ttattacaga
     3061 ttcatttgaa tattttttaa ccgttttcca gcgagataaa atactaccaa ttctgttaat
     3121 atcagataaa ccacccgcca atatttcagc aactggaaaa tttagataat aaaatttata
     3181 tccctttaaa taacatcgat aaataaattc aaagtccccg acaattttat tgtcaatatt
     3241 aaacttatat tctttaaaaa cctcaatatt ggtaaaaaga ctttgatgag aaaagaccat
     3301 tcccttccaa agttcttcaa tcttcctggc ttttgcaatt cttgaaaatc cactgttata
     3361 ggtaacttta caatcaccat atatgaaatc aacaaaatag tttttttgat aaaaaatatt
     3421 ttccaatgta ttgcttttat aaaacctatc acctgaattt aaaaatattg tccattcacc
     3481 tttagccaac cctgcaccct tattcatagc atcatagaca cctttatcgg gctcactaat
     3541 caaaatacta atatgttctt tataattctg aattattttt aaagtgttat cagttgaacc
     3601 accatctact ataatgtact catatttttt atatgtttga tttaaaactg attcaatagt
     3661 atcggtaata aacttctcgc tgttatagca aacagtaatt atcgaaacta agggaagtaa
     3721 attatctgat atagcttcat tttgtacttt tttatccata atttagcctt gtccttgagg
     3781 tacattaatt taaattacac tatattttga atctaacgag aagattatta gcaacaaatt
     3841 cattttattt tctgataaat tttagacaat aaacaatata gttccggata gattttaaat
     3901 agtaataatg caacatgcct ttttttaaat ccgacatctc tataaacttt tccatcaata
     3961 agagaataat aatttttaaa ctcataaatc agtttcttga aatatttttt ttgtttttct
     4021 ttaggaagtg aggcttttat ttctttatac aatctcaata tatgctctac aatatatagt
     4081 gctgcatatg aagataaatc ttcaaatctt gttctataga agtcatatac atctttacca
     4141 gataaaatta tatctaagct atcatcgcta aagggtgaat tgagtgcact ccttttgtgc
     4201 ttgacatagt aataataaca accttctata gttgcctttc tcttaattaa ataagcggct
     4261 atggcattta cccaataatc ttcaccaaat ctatatatag gaaatctaat acttttaaat
     4321 aaagtttttc tatataattt atttgcattg gaatgtgtag cattcttgcc ttgcaaaaaa
     4381 tctttactac aatcataatt tccattaata atattatttt ttagtgggaa agataaaata
     4441 accatgtatt gttcattaac taaattatag ccgcactcta taatatcgga atcatttttt
     4501 agtgttgctt cgattaatag tctatacata tctttgtgga tatagtcatc gctatctaca
     4561 aatccgatat aatctcctga tgctacatcc aagccgctat ttctggcaga agaaacacca
     4621 ccattt
//
LOCUS       DAOQWT010000055         4502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_19645_length_4502_cov_3.523049, whole genome shotgun sequence.
ACCESSION   DAOQWT010000055 DAOQWT010000000
VERSION     DAOQWT010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19645_length_4502_cov_3.523049"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PKJ95_01870"
     CDS             <1..1031
                     /locus_tag="PKJ95_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNR65021.1"
                     /translation="EAARILYQCRENLAELFNTSDPLRIIFTLNATESINLVLKGLLH
                     AGNHVITSSMEHNAVMRPLRELEKCGVQVQIVPCSCDGYLDPLDIEKAIKKNTALIIL
                     NHASNVTGTLLPIRSVGAIARKRNILFLVDGAQTAGTYPIDIEKDNIDLLAFTGHKSL
                     FGPTGTGGLVIGEKVDINKIAPLKTGGTGSRSESENHPDFLPDLYESGTSNIVGIAGL
                     KAGIRYILKRGITEIRQQERALTDKLISGLKEIPEVILYGKEGTGNRVAVVSFNTRNQ
                     SPSDIGLKLDEEYDIMCRVGLHCSPASHRTIGTFPAGTVRFSLSVFNTEQEIEKAVMA
                     VKNITKIL"
     gene            1040..1291
                     /locus_tag="PKJ95_01875"
     CDS             1040..1291
                     /locus_tag="PKJ95_01875"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HNR65022.1"
                     /translation="MNRKVDGMTEPLQKSVVIFYSTSAAIKAESLAKKQSLTVKLIPV
                     PRHLSSDCGICLSFNSNDIQRMEGILNYNKIEYDSTYNL"
     gene            1506..3449
                     /locus_tag="PKJ95_01880"
     CDS             1506..3449
                     /locus_tag="PKJ95_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR65023.1"
                     /translation="MTELSPTDKPPQRKKKSKERKSFLGPVADLEQHVRPDWWSKIFN
                     YLYLKTDGDVVDDSSITSSEIDSFLTILKPSSDDRILDLCCGQGRHSMELSRRNFQNI
                     EGLDRSHYLIQKAKSIAKKEDLKVKFKEGDARKIPFSNDSFNIIMVLGNSFGYFDTIQ
                     DDLRVLKEVFRTLKPWGKLLIQVTDGEYLKEHFLPRSWEWIDKKHFVCRERSLSLDKQ
                     RLISREVVTHIEKGIIADQFYAERLYSQENLSELLTAAGFSEMTFHGIMSPDSKRNQD
                     LGMMERCLTVTAVAKKEWTPVRKKERRQLKKVVVLLGDPSKTDPLKPSAIFDDDDLYT
                     IDQLKEELGELKNYLFSYLNNHDNLINDLIKLKEKDKIDIVFNLCDEGYDNEARKELH
                     VPSLLEMINLPYTGSGPQCLAFCYDKSLVRGIAREMGIPVPEAFFIKPNDVIFELSFN
                     FPVIIKPNLGDSSFGITQRSVANNFEEIVNAISEIREKFGYDKPILVEEFLTGKDLSV
                     GILGNPPESYNVLPIIMADYSDLPEDLPKICGYESKWLPDTPYWSIKSVPADLPENTE
                     KFIIECCLKLSERLECRDYCRFDWRLDSKGIPKLLEVNPNPGWCWDGHLAKMAEFAGI
                     SYGEMLKLILEASEQRLSQSKTT"
     gene            complement(3576..4382)
                     /gene="cobS"
                     /locus_tag="PKJ95_01885"
     CDS             complement(3576..4382)
                     /gene="cobS"
                     /locus_tag="PKJ95_01885"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNR65024.1"
                     /translation="MSNITRFVRRFLLALSFLTVLPIPLIFFKKEIENNNSVNADFSK
                     SSVFFPLVGLIIGGILHIFCLLLKYIELPVHLQAGLLLTIWVFLSGGLHLEGLADMVD
                     GFSGGKNKEEIIAIMKDGSIGAKGAIVLILLIMLKYLLLLSLAEHAKWVILLLAPMLG
                     RWSMIIAAYSGKPASSSNTLSNMFTRYLGKKELLISTLFVVMVMVSLLMFSGQLIFEP
                     LILLLINIGMTVIMVTYSKQKIGGICGDVIGAANEINELAVLLVSFLIIN"
     gene            complement(4375..>4502)
                     /locus_tag="PKJ95_01890"
     CDS             complement(4375..>4502)
                     /locus_tag="PKJ95_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNR65025.1"
                     /translation="PTGRFYRDILGQANQSIARNADLVYLLVAGIPLLIKGNKDE"
BASE COUNT         1541 a          807 c          853 g         1301 t
ORIGIN      
        1 tagaggcagc aagaatattg tatcaatgcc gggaaaacct ggctgaattg tttaatactt
       61 ctgatccttt gagaattatc ttcacactga acgcaacaga atcaatcaat ctggtattaa
      121 aaggattgct tcatgcaggg aaccatgtta tcacaagcag tatggaacac aatgctgtca
      181 tgcgcccctt acgggagctt gaaaaatgcg gggttcaggt gcagattgta ccctgctcct
      241 gtgatggata cctcgatccg cttgatatcg agaaagctat taaaaaaaat accgccttaa
      301 ttatcctgaa ccatgcctcg aatgttaccg gaacattgtt gcccattcgt tctgtgggag
      361 caattgccag aaaaagaaat attctttttc tggtagatgg ggcgcagaca gccgggacat
      421 atccaataga cattgagaaa gataatattg acctgctggc ttttaccggc cataagagct
      481 tatttggacc aacgggaacc ggagggcttg taattgggga aaaagttgat attaataaaa
      541 ttgccccttt aaagacaggt ggtactggta gccgttcaga atcagaaaat caccctgact
      601 ttctccccga tttatatgaa agcgggacat caaatatagt gggaatagcg ggattgaagg
      661 caggtatccg ttatatttta aaaagaggaa taacagagat acgccagcag gagagagcct
      721 taaccgataa attgatttct ggtttaaaag aaatacccga agttatcttg tatggaaaag
      781 agggaacagg aaaccgggtc gccgttgttt cttttaatac tcgaaatcag tctccctctg
      841 atattggatt aaaacttgat gaagaatatg atattatgtg tcgtgttgga ttacattgct
      901 caccggcctc tcacaggact attggcactt tccctgctgg tacggtccgt ttcagtttga
      961 gtgtattcaa tactgagcag gagattgaaa aagctgttat ggctgtaaaa aatattacaa
     1021 aaatactatg attattataa tgaatagaaa ggttgatgga atgactgagc cattacaaaa
     1081 aagtgtagtc atattttatt caaccagtgc tgctatcaaa gctgagagcc tggcaaaaaa
     1141 gcagagcctt actgttaaac tcatacctgt acccagacac ttaagctctg attgtggtat
     1201 atgccttagc tttaactcca acgatatcca aaggatggag ggaatattaa attataacaa
     1261 aatagaatat gattctacct ataacttata aatcataaaa taatctttta gtgcataatg
     1321 cgttgtagta catattttat tctaactata tctaccatat aaattaatat ttatccctgt
     1381 tgagttgtgc aaaaaatgct acatttatta atgaaaataa atgcctaaaa ataatacttg
     1441 taatagggga aaaagatatt ataattaact taaaaataat ttttatgaaa gaaggtaaat
     1501 aataagtgac tgagctatca cccactgaca aacctccaca gcgaaagaaa aaaagtaagg
     1561 aaagaaaatc gttcctggga ccggttgctg atttagaaca acatgtaaga cctgactggt
     1621 ggagcaagat atttaactat ttatatttaa agaccgacgg agatgttgta gacgattcaa
     1681 gtatcacttc cagtgaaatt gattcatttt taactattct caaaccctct tcagatgata
     1741 ggatccttga cctctgctgc ggacagggta gacattctat ggaattaagc agaagaaact
     1801 ttcaaaatat tgagggatta gaccgctctc attatctaat ccaaaaagct aaatcaatag
     1861 ctaagaaaga agatctgaag gttaaattta aagaaggaga tgcccgcaaa ataccattca
     1921 gcaatgattc ctttaatata ataatggttt tgggaaacag tttcggttat tttgatacca
     1981 ttcaggatga tttaagagta ttaaaagagg tatttagaac ccttaaacca tggggaaaat
     2041 tactcattca ggttaccgat ggggaatact taaaagaaca cttcttaccc cgttcctggg
     2101 aatggataga taaaaaacat tttgtctgcc gggaacgctc tctctcttta gataaacaac
     2161 gtcttatttc cagagaggta gttactcaca tagaaaaagg aataatagct gatcaatttt
     2221 atgcagagcg cctctattct caggaaaatc tgagcgagct tttaactgcc gctggtttta
     2281 gtgaaatgac ttttcatgga ataatgtccc ctgattctaa aagaaatcaa gacttaggaa
     2341 tgatggaaag atgtttaact gtcactgctg ttgctaaaaa agaatggacg ccggtccgga
     2401 aaaaagaacg caggcaatta aaaaaagtag tggttcttct aggagaccct agcaaaacag
     2461 atcccttgaa accatcagcc atcttcgatg atgatgacct ttacacaatc gatcagttaa
     2521 aagaagagtt gggtgaatta aaaaactatc ttttttctta tttaaataac catgacaatt
     2581 taattaatga tttgatcaaa ctgaaagaga aagataagat agacatagtt tttaacctct
     2641 gtgatgaggg atatgacaat gaagctcgaa aagaattgca tgtcccatct cttttggaaa
     2701 tgattaacct tccctatacc ggctccgggc cacagtgtct ggcattttgc tatgacaaat
     2761 ctctggtacg cggtattgcc cgggaaatgg gtattcccgt accggaagcc ttttttataa
     2821 aaccgaatga tgttatcttt gagttatctt tcaattttcc agtcattatc aaacctaact
     2881 taggggattc cagtttcggt atcactcaac gcagcgtagc caataatttt gaagaaatag
     2941 taaatgctat ttctgaaatt agagaaaaat tcggctatga caaaccgatc ctggtagaag
     3001 aatttctgac cggaaaagat cttagtgtgg gaatactggg gaatcctccc gaatcttata
     3061 acgtgttgcc cataattatg gccgattact ctgatttgcc ggaagatcta cccaaaatat
     3121 gcggctatga atctaaatgg ctgcctgata ccccttactg gagtatcaaa tctgtaccgg
     3181 ctgatcttcc tgaaaatact gaaaagttta ttattgaatg ttgtttgaaa ttatcagaaa
     3241 gattagaatg cagagattat tgccgatttg actggagatt ggacagtaag ggaataccaa
     3301 agcttttaga agtaaatcct aatccgggat ggtgctggga cggacatctg gctaagatgg
     3361 ctgaatttgc cggaatatcc tatggagaga tgctcaaact gattttagaa gccagcgaac
     3421 aaagattaag tcaatctaaa acaacataat ttttagggta agttcagctt taatattcac
     3481 catttattca caaaaattat aagacctggg atttgatata ttatttttta atcagtgagg
     3541 gggttagcta tccacaaagt tatagaacta accctctaat ttattatcaa aaaagagact
     3601 aataatacag ccaattcatt tatttcattg gcagcaccga ttacatcacc acatattccc
     3661 cctattttct gcttggaata cgttaccata ataacagtca ttcctatatt aattaacaat
     3721 agaataagtg gttcaaatat aagctggcca gaaaacatta aaagactgac catcaccata
     3781 actacaaata atgtcgatat taataattct tttttaccca gatatcgggt aaacatattt
     3841 gacaaagtat ttgaggagga tgctggtttc cctgaatatg ctgctatgat catagaccat
     3901 cttcccagca taggagctaa aagcaaaatt acccatttgg catgctcggc taagctgagc
     3961 aaaaggagat attttaacat tattaataaa attaagacaa ttgcaccttt cgccccaatt
     4021 gagccatctt tcataatagc aataatttct tccttatttt tgccaccaga aaacccatcc
     4081 accatatctg ctaacccttc cagatgaagt cccccactta aaaataccca tattgtcagc
     4141 agtaatcctg cctgcaggtg aacaggtaat tcaatgtatt ttaacagcag acaaaaaata
     4201 tgaagaatac ctccaataat taaacctacc agaggaaaaa agacactgga tttagaaaaa
     4261 tctgcattaa cactattatt attttctatc tcttttttga aaaaaattaa aggaatgggt
     4321 aaaacagtca aaaaagagag tgctaacaaa aatctgcgga caaaacgggt aatgttactc
     4381 atctttattg ccttttatta atagtgggat acctgctacc agaagatata ctaaatcggc
     4441 attacgggca atagattgat ttgcctgacc caatatatcg cgataaaacc gacctgtggg
     4501 at
//
LOCUS       DAOQWT010000056         4498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_19671_length_4498_cov_3.336935, whole genome shotgun sequence.
ACCESSION   DAOQWT010000056 DAOQWT010000000
VERSION     DAOQWT010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19671_length_4498_cov_3.336935"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PKJ95_01895"
     CDS             <1..717
                     /locus_tag="PKJ95_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNR65026.1"
                     /translation="LSAFISAMLSIFSLMNPLEKFVTFIIRIILKIICKVEIDKPESI
                     PQEGPLLFVFNHINFLEVPLIYLLLQPRNVVAMVKKENWDNWFYRILANAWGGIPVDR
                     EKPKVSTFREIEKAIKNNSIICIAPEGTRSETGVLGKGHLGAVFLALHYQIPILLMAH
                     YGSEKFWYNFKRFKRTLVTFKIGTPFMLVGNSKIDKNLQQEMNEQIMTRIASMLPEPY
                     RGTYKNLDIISEKYIKYLTE"
     regulatory      908..1025
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            1091..2113
                     /locus_tag="PKJ95_01900"
     CDS             1091..2113
                     /locus_tag="PKJ95_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR65027.1"
                     /translation="MFKKRRFLFLLVILAAILFLSSLSVLSEEIVLVDVIGREVVLEE
                     PAIKVVGTHNPTLNAAVVLGGGRKYIAGFGNKEMSRGLYEAVIDDYDGLVQIGKGGNI
                     NFETVLATGADLAIIPERFKDQVEQFEKVGMKVIVALPNEESFDTIRQSLTLLGKALG
                     ATDRANLILSYLDDRIENAQKISSTVTEKKKVLFLGGSSALSVAPAAMIQTQLIDIAG
                     GENAVSGIEGTGDFVEVNIEQIINWNPDVIWYPAYASYSAEDLLNNPSWSSIKAIQNK
                     EIYAFPSKIEPWDQPTAALALGIAWATHNLHPDLYTIDEVMKDVDEFYNLVYGITFSA
                     ERLGIK"
     gene            2143..3153
                     /locus_tag="PKJ95_01905"
     CDS             2143..3153
                     /locus_tag="PKJ95_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007243556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNR65028.1"
                     /translation="MNKSRYRQQLMLLSMLLFISFFVSLSLGRYSNNPFDVLKVVLSK
                     AGFNQSRDSVMESIIYYIRMPRIVVSILVGAMLSLSGAVYQGVFKNPLISPDLLGVSS
                     GASVGAALAIMLGASLAQRQVMAFLGGLFAVYLSTSIPKLFKNRNNMMLVLSGIIVSG
                     FLCSILAIMKFIAEEQTELASIVFWQMGSMAKVLGREIIAISPVFLACTLTLILLSWR
                     INILSFGDLEAKTLGININQIRGIVIVCASLLTASAVSVSGTIGWVGLVIPHLGRILV
                     GTDHTRLIPTTVVMGALFMLVIDTLARTISNVEIPLSILTGFVGAPLFAYLLYRQRTL
                     AE"
     gene            3164..3961
                     /locus_tag="PKJ95_01910"
     CDS             3164..3961
                     /locus_tag="PKJ95_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65029.1"
                     /translation="MKIKVEQVGFSYDRDKEIFSGINLDVNSGEVVSILGPNGIGKTT
                     LLSCLAKIRIPTSGTIFLNGQNMSKMGHRDIARIIGYVPQTIFPSFDYDVLDYVVTGL
                     SPWLGLFEKPKEEHYQLAQNSLKKMGIEYLKRKPYSKISAGELQQVSIARVITQKVQL
                     ILMDEPTAHLDYGNQLKVLGIIKEMAEEGYGVVLTTHNPDQVLLLKSKVAVFDRKGQF
                     HFGNWDEVITDDLLEEIYGVKIQICDVEKVQRKVCIAPKIPKTSRRT"
     gene            3966..>4498
                     /locus_tag="PKJ95_01915"
     CDS             3966..>4498
                     /locus_tag="PKJ95_01915"
                     /inference="COORDINATES: protein motif:HMM:NF025306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNR65030.1"
                     /translation="MLINKVLAEAKPYLINRTIKDAVLGISLIGIELDNKDIGLAYML
                     REHLPAGCSVFGFGQELIGTNAYKVAQLAQNGSDDAQRGVGIAVLTAGSRQLTLPDED
                     KNEPFFGLEINSYDVVGMIGYIPPVAKRFAEKIKDLIVFDEDISRCGGESIEYIKPMA
                     KQEELLPNCDVVIITGTT"
BASE COUNT         1437 a          680 c          940 g         1441 t
ORIGIN      
        1 ttatcagctt ttatttctgc aatgttatca atattttcat taatgaatcc attagaaaaa
       61 tttgtaactt ttattataag aataatatta aaaataatct gtaaggtaga aattgataaa
      121 ccagaatcta tcccgcaaga agggccttta ctgttcgtct ttaatcatat caattttctg
      181 gaagttccgc taatttatct gcttttacag ccccgtaatg ttgtagctat ggtaaagaaa
      241 gagaattggg acaattggtt ttatagaata cttgccaatg cctggggagg tattccggtg
      301 gatagagaaa aaccaaaagt atcaaccttt agagaaatag aaaaagctat taagaataat
      361 agtattattt gcattgctcc ggaagggacc agaagtgaaa ccggggtatt ggggaaagga
      421 catttagggg cagttttttt agccctgcat tatcaaatac ctattttgtt gatggcacat
      481 tatggttcag aaaaattttg gtataatttc aaaagattta aaaggacact ggtaaccttt
      541 aaaattggaa ctcccttcat gctggtaggt aattcaaaga ttgataaaaa tcttcaacag
      601 gaaatgaatg agcagatcat gactcgtatt gcttcaatgc taccggagcc ctatcgcggt
      661 acctataaaa atttggatat catttcagaa aaatatatta aatacctgac agaataatat
      721 taaaaaataa aagatttaaa ttactccatt ctgaacattc tcttaattta gacttttctt
      781 aaatttaaat atttataaat aaatacccat ttctgattaa aaattggaac catcaaagac
      841 tttttgggtg tcaaagactt tatgggttga catgctaatc aattgatgtt atatttttca
      901 ttggtaaaat atttaccgca tccgagttgt tacttctaag ggttcgccta tgggtaacag
      961 cctattggta catcaggaaa cgagtgtacc acccgtgctt ggaaaggatg aaaaaaagaa
     1021 taaaaaatca tcaaaataca aagtaacggg aacgttactt tttttatttt aaaaaaaaga
     1081 ggagttctat atgtttaaaa aaagaagatt cttattttta ctggtaatac tagcagcgat
     1141 acttttctta tcaagcttgt ctgttttaag tgaggagatt gttctggtag atgttattgg
     1201 tagagaggta gtgttggaag aaccggcaat caaggtagta ggtacccata accctacctt
     1261 aaacgctgca gtagtccttg gtggtggaag aaaatatatt gccggatttg gtaacaagga
     1321 aatgtccagg ggactttatg aggcagtgat agatgattat gatggattgg ttcagattgg
     1381 gaaaggtggc aatattaatt ttgaaactgt cctggcaacc ggagctgatc tggcaatcat
     1441 tcctgaacgt tttaaagatc aggtagaaca gtttgagaaa gtgggaatga aagtgatagt
     1501 agcattacct aatgaggaga gctttgatac catcagacaa agtcttactt tattaggcaa
     1561 ggctttaggg gctaccgata gagcaaatct aattttaagc tatttagatg accgtattga
     1621 gaatgcccaa aaaatttcat caactgtaac tgaaaagaag aaagttttat tcttgggtgg
     1681 aagctcagca ctttcagtag ctcctgctgc tatgattcag actcaattaa ttgatattgc
     1741 tggaggcgaa aacgctgttt ccggtataga aggaaccgga gattttgttg aggttaatat
     1801 tgaacagata attaattgga atcctgacgt tatctggtat cccgcttatg ctagttattc
     1861 agcagaagat ttattaaaca atccttcctg gagcagtatt aaagcaatac aaaataaaga
     1921 aatatatgct tttccctcca aaattgaacc gtgggatcag cctactgctg ccttggctct
     1981 tggtattgcc tgggcaaccc ataatttgca tccagatctg tacaccattg atgaggtaat
     2041 gaaagatgtc gatgaattct acaatttagt ttatggtatt acttttagtg cagaaaggtt
     2101 aggcattaaa taaccggttg actatatttt gaaaggtaga gcatgaataa atccagatat
     2161 agacaacagc taatgctgtt gtctatgctt ttatttataa gtttttttgt ttccttaagt
     2221 ttaggacgtt atagtaataa tccttttgat gttttaaaag tagttttatc aaaagcaggt
     2281 ttcaaccaat ccagagattc agtgatggaa agtataattt attatatccg aatgccaagg
     2341 attgtagtat ccattctggt tggagctatg ctctcactat ctggtgcagt ttatcaggga
     2401 gtttttaaga atcccctgat ctcgcctgat ttacttggag tttcttcagg tgcttcagtt
     2461 ggtgcagctc tagccataat gttaggggca tctcttgccc aaagacaagt aatggctttt
     2521 ttaggagggt tatttgcagt atatttaagt acctcaattc ccaaattatt taagaataga
     2581 aataatatga tgctggtcct ttctggtatt attgtcagcg ggtttttatg ttcaattcta
     2641 gccattatga aatttatagc agaagaacag actgaactgg cctctatagt tttctggcag
     2701 atgggtagta tggccaaagt gttgggcaga gagataattg ctatcagtcc agtcttcctg
     2761 gcctgtaccc tgacattaat acttctttcc tggagaatta atattttatc ttttggtgat
     2821 ttagaagcaa aaacactggg aataaatata aatcagatta gaggaattgt tattgtctgc
     2881 gcaagtcttc tgactgctag tgctgtgtca gtaagtggaa caataggttg ggtaggtctg
     2941 gtaattccgc atctggggcg tatcttagtt ggcacggatc acaccagact gataccaact
     3001 actgtagtta tgggagcctt gtttatgctg gtaattgata ctcttgcccg tactatctct
     3061 aatgttgaga ttccactttc aatattgacc ggttttgttg gtgcacctct ttttgcctat
     3121 ctgctttata gacagagaac acttgctgaa taggagagtg tggatgaaaa taaaagttga
     3181 acaagttggt ttttcttatg acagggataa agaaatattc tctggtatta atttagatgt
     3241 taattcaggt gaagtagtta gcatcctggg accaaatggt ataggtaaaa ccactctact
     3301 ttcctgcctg gctaaaataa gaattccaac cagtggtacg atatttctta atggtcaaaa
     3361 tatgagcaaa atgggacatc gcgacatagc gcgtattatc gggtacgtac ctcagacaat
     3421 ttttccttct ttcgattatg atgttcttga ttacgtggta acaggattat ctccctggct
     3481 tgggttattt gaaaaaccta aagaagagca ttatcaattg gcacaaaatt ctttaaaaaa
     3541 aatgggaatt gaatatttga agagaaaacc ttattctaaa ataagtgcag gagaattaca
     3601 acaggtttca atagccagag taattactca aaaagtacag cttattttga tggatgaacc
     3661 tacagcacat cttgattatg gtaatcaatt gaaagttttg ggaattatta aggaaatggc
     3721 cgaagaaggt tatggagttg tcctgactac tcataatcct gatcaagtac tgttgttaaa
     3781 aagcaaggtt gctgtttttg atcggaaagg tcaatttcat tttggcaatt gggatgaggt
     3841 tatcacagat gatttactgg aagaaattta tggtgtaaaa atacaaatat gtgatgtaga
     3901 gaaagtgcaa agaaaagttt gcattgcacc aaagattcca aaaacaagta gaaggacgtg
     3961 attttatgtt aattaataag gtattagcag aagcaaaacc atatctgatt aatagaacaa
     4021 taaaagatgc cgttcttggt atatctttga taggtattga attagacaat aaggatattg
     4081 gtcttgctta tatgttgcgg gaacatctgc ccgccgggtg ttcagttttt ggttttggtc
     4141 aggaattaat tggtacaaat gcctataaag ttgcacagtt ggcccaaaac ggttcagatg
     4201 atgcacaaag gggagttggg atagccgtac ttaccgcagg atcccgccaa ctgaccctcc
     4261 ctgatgaaga taagaatgag cctttttttg gtcttgaaat taattcatat gatgtagtag
     4321 gaatgatagg ttatattcca cctgtagcca aaagatttgc tgagaaaata aaagatttga
     4381 tagtattcga tgaggatatt tctcgttgtg gtggcgagag tattgaatat attaagccaa
     4441 tggcaaaaca ggaagaactt cttcctaatt gtgatgtagt cataattacc gggacaac
//
LOCUS       DAOQWT010000057         4484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_19755_length_4484_cov_3.300971, whole genome shotgun sequence.
ACCESSION   DAOQWT010000057 DAOQWT010000000
VERSION     DAOQWT010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19755_length_4484_cov_3.300971"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PKJ95_01920"
     CDS             complement(<1..892)
                     /locus_tag="PKJ95_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65031.1"
                     /translation="MEQILKHLQNISQKYPFHKKTILAPSYLDGNAFKKSLTLKGFSA
                     LNFDITTLFDVARDIYFPVLLKNEWKILDSTIGQILVFDILKALSSEEKLSYFKLPLL
                     TPSLAATIFRTIKEIRVSGYSAHNFPQKIQTVSHKMRDLFLIIVEYERKLKEERLLDE
                     AELYSLAERLKPKKEEIIYLIPANIPMNEMELRFYKKRIKPKSILLHFSCPEASTAPH
                     SFPITAYGDSSQSYEEYFYNILQHRENIDFQQLPSLDLEFVQTYGEYNETREVLRKIM
                     IEGYPFDKVQVFYTTQEPYSQ"
     gene            complement(921..1346)
                     /locus_tag="PKJ95_01925"
     CDS             complement(921..1346)
                     /locus_tag="PKJ95_01925"
                     /inference="COORDINATES: protein motif:HMM:NF016555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNR65032.1"
                     /translation="MLEKCPFCQIINREKEAYIVYEDEKCMAFLDAYPVTEGHTLVIP
                     KEHYENIYEIPEEYLAHLIRICKRLALDYKKIFQTIGLNIIQSNGIAAKQTIFHFHIH
                     LVPRYEQDGLSLFRHHFPRSEDNLSRAYHKIVNFQREIK"
     gene            complement(1404..2342)
                     /locus_tag="PKJ95_01930"
     CDS             complement(1404..2342)
                     /locus_tag="PKJ95_01930"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNR65033.1"
                     /translation="MATVIVIGAINLDCLVYTPHFPNKGDNIRVKDMKCSLGGRGANQ
                     AVALTNLKVAALLMGKVGNDFAGDYALSILKKYKVNTEYVLKSSTGKTGLCSILVSPD
                     GENTIMGFPAINRTVEPDFLMRFEYLFEIAQWLAISLEYPLDVVELALKLARKYGLKT
                     VLDPSPLIELPKQNIWNMVDYVIPNKKEIGILTDEEEMLQGAIVLKNWGVGEVIVKQG
                     SQGCSFLHQDNLINVPAFPVKAVLDTTGAGDLFDAAFLYGLIQSNSVAKAAQIGNLVA
                     SYAVQKPGTCESFPERREIDWEWLERRKKGLFNQQD"
     gene            complement(2503..3858)
                     /locus_tag="PKJ95_01935"
     CDS             complement(2503..3858)
                     /locus_tag="PKJ95_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HNR65034.1"
                     /translation="MHQNWSLVMDFLWLSLFIGIGVFFKRKVYFFQKYLFPTAIIAGF
                     IGLILGSEVLKVINLDSERLGSLVYHLMAIGFISIALKKRENIHTRDIFNSGVYIVSI
                     YLIQGIIGFILSLLLVNTFFPDLFPTFGLLLPLGFGQGPGEAYAIGYQWEELGFYQGG
                     NIGLSIATIGFLWACIVGVVLMNYLIKKNKFTVELDKYTAREKIYEEMDPGELPLAAS
                     LDDISIQLFLIGLVYLVTYLTLLGLNKILLPLGTFGETLAQLLWGFHFIIGTLYAILL
                     RVIFNQLNRMKIMSHEYPNSYMLQRIAGGSFDFMITASIAAISLKTLHYYLVPTLLIT
                     TIGGIVTITFILWMTPRVFKEDVFQNIMVFYGTYTGTISTGMALLKQVDPGFHSGASD
                     NIVLGSGVALLFGFPLMIMLNIPIFGYVTNQPIMYLVALLAFFTYFAILYLILYKNRV
                     K"
     gene            complement(3878..4138)
                     /locus_tag="PKJ95_01940"
     CDS             complement(3878..4138)
                     /locus_tag="PKJ95_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65035.1"
                     /translation="MVDHFKKLEPWKDGQLLWQGKSILFHKNGKEYLNFELEHIKGIN
                     VQYNNRFDFYHKEQLYQFYFNCDSISAYKWETMIKLAQQIFF"
     gene            4160..4399
                     /locus_tag="PKJ95_01945"
     CDS             4160..4399
                     /locus_tag="PKJ95_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65036.1"
                     /translation="MNKNTCVVILLISLEKALSVINLPSIPVSRVIKIESFFTVSKEP
                     IYVIFYLITTFTTKKYIISGGECTNLLTVRTEVEI"
BASE COUNT         1416 a          854 c          756 g         1458 t
ORIGIN      
        1 actgagagta tggttcctgt gtagtgtaaa aaacctgtac cttatcaaaa ggataccctt
       61 ctatcataat ttttcttaaa acttctctgg tttcattgta ttctccatag gtttgcacaa
      121 attccaggtc tagggatggt aactgctgaa aatcaatatt ttctctatgc tgtaatatgt
      181 tgtaaaaata ttcttcatat gattgagaac tgtctccata agcggttatt ggaaaactgt
      241 gcggtgcagt acttgcttca ggacagctaa agtgaagcag aattgatttt ggttttattc
      301 tttttttata aaatcttaat tccatctcat tcattggtat attggcagga atcagataga
      361 ttatttcttc cttcttcggt tttaatcttt ctgctagaga gtagagttcg gcttcatcga
      421 gcaatctttc ttcctttagt tttcgctcat actctactat tattagaaac aggtctctca
      481 tcttgtgaga tactgtttgt attttttgag gaaaattgtg agcagaatag ccggaaactc
      541 ttatctcttt gatagttctg aatatcgttg ccgccagaga aggcgtgagt agtggtaatt
      601 taaaatagga taatttttct tctgaggaca atgcttttaa tatgtcaaaa accagtatct
      661 gtcctatagt gctatccagg atcttccatt cattttttaa taaaacaggg aaatatatat
      721 cgcgggcgac atcaaaaagt gtggtaatat caaaatttaa agccgaaaaa ccctttaaag
      781 tcaagctttt tttaaaggcg tttccgtcca gataagaagg tgccagaata gtctttttgt
      841 ggaaagggta tttttgactt atattctgta aatgttttaa gatttgttcc attaatcttc
      901 ctatttaacc gtggacaata ttattttatt tctctttgaa agtttacgat tttgtggtaa
      961 gcccgcgaga gattatcctc acttctggga aaatggtgac gaaaaagaga aagtccatcc
     1021 tgttcataac gtggaacaag atgaatatga aaatgaaata ttgtttgttt agcagcaata
     1081 ccattggatt gtataatatt tagaccaatg gtctggaata tttttttata atcaagggct
     1141 aatcttttac aaatcctgat taggtgggca aggtattctt ctggtatttc ataaatattt
     1201 tcataatgtt ctttcggtat taccagggtg tggccctctg taaccggata agcatccaga
     1261 aatgccatac atttttcatc ttcatagact atatatgcct ctttttctct attaattatc
     1321 tggcaaaagg gacatttttc caacatataa actcctctat caatttctgt ttaacttctc
     1381 atctacaggt gctctaaaac tatttaatcc tgctggttga atagaccttt tttccttctt
     1441 tccagccatt cccagtctat ttccctccgc tcggggaaag attcacatgt gccaggtttt
     1501 tgcactgcat aggaggcaac cagattgcca atctgggcag cttttgctac tgaatttgac
     1561 tgtattaatc catataaaaa ggcagcatcg aataaatccc cggcaccagt agtgtctaaa
     1621 acagctttta cggggaatgc tggtacatta atcaggttat cctgatgtaa aaaactacat
     1681 ccctgactac cttgttttac aattacttct cccactcccc aattttttaa tactatggca
     1741 ccttgaagca tctcctcttc atcggtaagg attcctattt cctttttatt tggtatgaca
     1801 taatctacca tattccatat attttgtttg ggcagttcaa tcaaaggaga gggatcgagc
     1861 actgtcttaa gtccatattt tcgggctaac tttagggcta attccaccac atctaaagga
     1921 tattccagag aaatagcaag ccattgggct atttcaaaca ggtattcaaa gcgcatcaaa
     1981 aaatcaggtt ctaccgttcg gttgatagcc gggaaaccca taatggtatt ctctccatcc
     2041 gggctgacca ggattgagca gaggccggtt tttccagtgc tgcttttaag gacatattcg
     2101 gtatttacct tgtatttttt taagatggaa agggcataat ctccagcaaa atcatttcct
     2161 acctttccca tcagtaacgc ggcaactttt aaattggtta gagctactgc ctgattggca
     2221 cctctccccc ctaaagaaca cttcatatct ttaaccctta tattgtctcc tttattagga
     2281 aaatgggggg tatagaccag gcaatccaga ttaatagccc ctattacgat aactgtggcc
     2341 atttttcttt accttatccc acttattttc tcacttattt tatattatgt gataagactc
     2401 acctaatgtc aaaattgctc aaaacttaaa ggagttttct tatataggta aatttatgat
     2461 gataattact ggtctggttt ttttaaagta gttaataaaa agctatttta ctctattttt
     2521 ataaagaata agatagagaa tagcaaaata agtaaagaaa gcaagcaatg caacaagata
     2581 cattatcggt tgattggtaa catatccaaa aataggaata tttaacataa tcataagtgg
     2641 aaagccaaat aaaagtgcca ctccactacc cagtacaata ttatctgaag caccagaatg
     2701 aaagcccgga tctacctgct ttaataaagc cattcctgta gagatagtac cggtataggt
     2761 gccataaaaa accatgatgt tttgaaaaac atcctcctta aaaacacggg gagtcatcca
     2821 gaggataaag gtaatagtta cgatgcctcc tatagtggta atcaatagag ttggaaccag
     2881 ataataatga agggtcttta aagaaatggc ggcaatagat gcggttatca taaaatcaaa
     2941 agaacctccc gcgattcttt gcagcatata gctattggga tattcatggc tcataatctt
     3001 catccgattt aactgattaa aaataaccct taataaaata gcatagagag taccgataat
     3061 aaaatggaat ccccacaaca gctgagccag tgtctcacca aaagtcccca gcggcaaaag
     3121 tatcttgtta agacccaata atgttaaata tgttactaaa tagaccagtc ctattaaaaa
     3181 cagctgaata gagatgtcat ctaaagaggc agccaaaggt aactcaccag ggtccatctc
     3241 ttcatatatc ttttctctgg cggtgtattt atccaactcg actgtaaatt tattcttttt
     3301 tattagataa ttcattaaga caacccctac aatacaagcc cagaggaatc ctatagtagc
     3361 aatggaaagt ccaatgtttc ctccttgata gaaccctaat tcttcccact gatagccaat
     3421 tgcataggct tctccaggac cttgcccaaa tcccagtggt aataacaatc cgaaggtcgg
     3481 aaatagatca gggaaaaagg tattgaccag caacagggaa agaatgaaac cgataatgcc
     3541 ctgtatcaga taaatgctga caatataaac accagaatta aagatatcac gggtatgaat
     3601 attctctctt tttttaaggg cgatggatat aaaaccaata gccatcagat ggtaaaccag
     3661 gctgcccaat ctttcactat ctaaatttat tacctttaat acctcacttc ccagtattaa
     3721 accgataaag ccggcaataa ttgcagtggg gaacaggtat ttctggaaaa aatatacttt
     3781 tcttttaaaa aaaaccccga taccaataaa caaagaaagc cataaaaaat ccataacaag
     3841 agaccagttt tgatgcatag ttcctccccc aattttgtta aaagaatatt tgctgagcca
     3901 gttttatcat agtttcccac ttataggctg agattgagtc acaattaaaa taaaattggt
     3961 ataattgttc tttatgataa aaatcaaacc ggttattata ttgaacattt atacctttga
     4021 tatgttctaa ctcaaaattt aagtattctt ttccattttt atggaaaaga atactctttc
     4081 cctgccataa aagctggcca tctttccatg gctctaattt tttaaaatgg tcaaccatct
     4141 ggcaatttac atctttttct tgaataaaaa tacctgtgta gttattctct tgataagtct
     4201 tgagaaggct cttagtgtaa ttaacctgcc atctattcca gtcagccggg ttatcaaaat
     4261 agagtctttc tttaccgtta gtaaagaacc catatacgtt atattttacc tgataaccac
     4321 atttaccaca aaaaagtaca tcatctctgg aggtgagtgt accaatttgc tgacagttag
     4381 gacagaggta gagatataat tctaatctct ccgccagatt tttaccctaa taagtattca
     4441 tagtctttct ctgataatca tattcgttgt aagaaaggct atca
//
LOCUS       DAOQWT010000058         4456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_19948_length_4456_cov_3.240400, whole genome shotgun sequence.
ACCESSION   DAOQWT010000058 DAOQWT010000000
VERSION     DAOQWT010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19948_length_4456_cov_3.240400"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..853)
                     /locus_tag="PKJ95_01950"
     CDS             complement(188..853)
                     /locus_tag="PKJ95_01950"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNR65037.1"
                     /translation="MKKRPVYLSAIIAILLTSFFMSGCMLPFLGNEPVTIVSKANLSA
                     IVGKLYKYQLDIEDDSKTKVVYTLDVAPEGMTMEGSTGLIMWTPKANQVGKHEVVIRV
                     SDGWFRDEQKFTITVTQFQLKSISIKPTTMSFPSIPSSKNIESITAKYNDGSSKDIEK
                     AKCEFKSNDPSVATVDEAGKVTSKKKGNTIITVSYTEEGITQSTNINITVTIDTDSSG
                     GGG"
     gene            complement(1095..1757)
                     /locus_tag="PKJ95_01955"
     CDS             complement(1095..1757)
                     /locus_tag="PKJ95_01955"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HNR65038.1"
                     /translation="MKQKHFCPLIVMVVLLTSFMVGVYVLSILANELTENLPETELKA
                     ISGIPLNYQLVLNDSNPKLIFVLRDAPEGMTIDRKTGMVEWTPTQEQIGVHKASIVVT
                     DGSNKDKQELTITVVSSGLSSIVAIPAVMDIEGVNITKKIESVTAYYSDGSSKVIDKT
                     KCHFQSDKTNIITVNERGIVETKNIGTATITVSYSEEGIAKNSTITVTVKLPTLPFFG
                     GG"
     gene            complement(2472..>4456)
                     /locus_tag="PKJ95_01960"
     CDS             complement(2472..>4456)
                     /locus_tag="PKJ95_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNR65039.1"
                     /translation="FRSLHSIGQFVNPLFRELFGNEESEYQVSYSSMQTTRADEKGYF
                     SGVYRYTIAKERNKKKEMENDARAIAMLIRSWIDNKVKILRTEEEFKSGQKYELEYRD
                     FMILLRYKSGMEIYSKLLSEYGIPVTVSGSAALNESKYLQELLKLLRLLKDPENQVLL
                     VAVLRGIFYGFSDEELYQYKVAIGGFSFFADTPFFLDNGLKQRFNDAFQQLKDYYSWC
                     FKYPPVVVLEKIMIHSGLLPYLCAEMNKKEEGNELFFILEYLRESEMRDYLTYDGMVE
                     RLERLWQSGLEEEFNMRVEENAVRLMNLHKAKGLEAPVVFLAIPYLTQKHEPEYHIER
                     HDIVPKGHFQIRKFNYFGKCKTIAQPQKWKEYGQIEKSYLEAEETRLLYVAATRAKNA
                     LVISSFSAGSQDNNKNPWQPLLKRTKSEMLLEIHSKDSTKLVTKTMSYGLEEYQTHMY
                     KREEAKNKLLARSYFESTATRQAEFSEIKSPLIPTVDKGGKKWGNAVHAILEQVVSEE
                     TEDGLLKAHIIDTLKRNSIDPFRKDELYGIVRKFIASDLYHRIKNALQKFTEVPFNIK
                     VVPSDPFYQELISEDREKTGEEGKPLILTGTIDLIFREADGWVVVDYKTDCPVKQSDY
                     VKLRQLYEVQISTYCTIWQKISGERVKESLIYFVSE"
BASE COUNT         1323 a          879 c          702 g         1552 t
ORIGIN      
        1 ggcggcggct gggaaagagg ttcggttgtt gtgtagattt gaaccgacgg cggaactttt
       61 aaaaaaacag cccacggatt tgaagtccgc aaggtccacc agaacctatc cactcccgct
      121 atctattata ctatattttt attctttagt cctgataaac tatcagggta tgtttttatt
      181 ttcttcctca gcctccgcct ccactgctat cagtatcaat agtaaccgta atattgatat
      241 ttgtactttg ggtaattccc tcttcggtat agcttacagt aataattgta ttacccttct
      301 tttttgaagt tacttttccc gcctcatcaa cagtggctac gctggggtca ttagacttaa
      361 attcacattt tgctttctca atatccttgc tgctgccatc attatatttg gcagtaattg
      421 actctatatt tttgctgcta gggatactgg gaaatgacat agtagtaggc ttgatactaa
      481 tggattttag ctgaaactga gtaacagtta tagtaaattt ctgttcatcc ctgaaccagc
      541 cgtcacttac tctgattaca acttcatgtt tgcccacctg atttgccttt ggtgtccaca
      601 ttattagacc ggttgaacct tccatggtca taccttcagg agcgacgtcc agtgtatata
      661 ccactttagt tttgctatca tcttcaatgt ccaactgata cttatagagt ttgcctacaa
      721 tagcacttaa gtttgctttt gatacaatag ttaccggttc atttcccaga aagggcagca
      781 tacatccact cataaaaaaa gaagttagca gaattgcaat aatcgctgat aaatagacag
      841 gtctcttttt cacattatca cctccttcca aacctttact taaatatata atttatcaaa
      901 aaaatgcttg tatatcagaa ttttaaaaaa caaattcgtt gaaatgaata tgcagacaat
      961 taattataaa aatattataa cacctgctgt caatttttaa tctatatcta tttttattat
     1021 ttattctctt ttacaaattc ttcttttatg acatttttta ggctgataga ctatcagtgc
     1081 tgttttttta actgtcaacc gccgccaaaa aaaggaaggg ttggaagttt tacagttacg
     1141 gttatggtag aatttttggc aattccttct tcgctataac ttactgtaat ggtagctgta
     1201 ccaatatttt ttgtttctac aattcccctc tcatttaccg taataatatt agttttatca
     1261 gactgaaaat ggcattttgt cttatcaatt actttactgg aaccatcgct atagtaagca
     1321 gtaactgact ctattttttt agtgatgttt acaccttcta tatccataac cgcaggtata
     1381 gcaactatag aactcaaccc tgatgaaacc actgtgatag ttaattcttg tttatctttg
     1441 ttggagccat cggttactac aatacttgct ttatgtaccc ctatctgttc ctgggttgga
     1501 gtccattcta ccattccggt tttccgatca attgtcattc cttcaggggc atctctaaga
     1561 acaaaaatca atttagggtt actatcatta agaactaatt gatagttaag gggaatcccg
     1621 gaaatagctt tcagctcagt ttcgggaaga ttctctgtca attcattagc cagaatagat
     1681 aacacgtaaa cgccaaccat aaaagaggtt agtaagacta ccataacaat caatgggcag
     1741 aaatgttttt gtttcatatt attatctatc tcatcttttt tattatctat attctctata
     1801 tatttggaat aacctatgtt ttataattta taaaattata tatatttacc aaaaattcaa
     1861 atatttaatg aaattaatta taacattttt atcaatgatg gtcattgttt attaatttac
     1921 tcaaacagat agtataaaaa cttttcaatt acctgataat tgaaccggta atacaattat
     1981 tgacattatt atatttcatg ttattatata atcctgaatg aacataccgg ggttgaatag
     2041 aaatcccaga attattacag aattattatg aggagaaaat attaatggaa ttaaatatta
     2101 ccgaaaaagc agcaaaacag ctgaaagata tgctccagga acaagaacaa tctgacaaaa
     2161 aattccgtat tgtgatgacc ggcatcagct gaaggggacc gagatttaac gttgctctgg
     2221 atgagcagca agaaaacgat tcacatttaa aggtagatgg ttttgatttt attatagacc
     2281 agaagctggt agaaagtatg gtctctttga ccattgatta taaaaattcc tttttatgga
     2341 aaggttttca ggttgattac aggtcggaat attcagggtt ttgttaaaac cgaataaaca
     2401 tttgagactg ctgactgaat catttataat ttaaaacaat aagaagagtc gggcataaac
     2461 cccggctctt cttattctga tacaaaataa atgagactct cttttaccct ttccccactt
     2521 attttctgcc agattgtgca ataggtagag atttgcacct catataactg ccgcagcttg
     2581 acataatcgc tctgttttac cgggcagtca gtcttgtagt ccactactac ccatccgtcg
     2641 gcttcccgaa aaataaggtc aatggtaccg gttagtatga gtggctttcc ttcttcccct
     2701 gtcttttctc tatcttctga aatcaattct tgataaaaag ggtcactggg aaccaccttt
     2761 atattaaagg gtacctcagt gaatttttgc agcgcatttt ttattctgtg atacaaatca
     2821 cttgctataa acttcctgac aataccatac aattcatctt tacggaaagg gtcaatgctg
     2881 tttcttttca gagtatcaat tatatgagcc ttgagcaaac catcttctgt ttcctcactc
     2941 actacctgtt ccaatattgc atgtactgca tttccccatt ttttaccccc tttatcaaca
     3001 gtaggaataa ggggtgattt aatttccgaa aattctgcct gccttgtggc agtactctca
     3061 aaatagctcc tggcaagtaa cttatttttt gcttcttctc tcttatacat atgggtctgg
     3121 tattcttcta agccataact catcgtttta gtcaccaatt ttgtagaatc cttgctatgt
     3181 atttctaaaa gcatttccga tttagttctt tttagtaaag gttgccatgg atttttatta
     3241 ttatcctgac tacctgcgct aaaactactt ataaccaatg cattttttgc tctggtagca
     3301 gctacataaa gcaatcgggt ctcttccgct tccaggtaag atttttcgat ctgaccatac
     3361 tctttccatt tttgaggttg ggcaattgtt ttacatttac caaaataatt aaatttccta
     3421 atttgaaaat gtcctttcgg tacaatatcg tgacgttcaa tatggtattc cggttcatgt
     3481 ttctgggtaa ggtaaggaat agccagaaaa acaacggggg cttccagtcc tttggctttg
     3541 tgtaaattca ttaatcgaac tgcattttct tctaccctca tattgaactc ttcttctaat
     3601 cctgactgcc ataatctttc cagccgttcc accattccat cataggttag ataatctctc
     3661 atctctgatt cacgcaaata ttccaggatg aaaaagagtt cattaccctc ttccttttta
     3721 ttcatttccg cacaaagata gggcaacaat ccggaatgaa tcataatctt ttctaatacc
     3781 accaccggcg gatatttaaa acaccaggaa tagtagtctt ttaattgttg gaaagcatca
     3841 ttaaatcttt gttttaaacc attatccaga aaaaaaggtg tatctgcaaa aaaactaaaa
     3901 cctccgatag ccaccttgta ttgatagagt tcctcatcag agaagccata aaagatgccc
     3961 cttaacactg ctaccaataa tacttgattt tcaggatctt ttagcaatcg taataatttt
     4021 aataattcct gaagatattt cgattcgttt aaggcggcag agcctgatac tgttactgga
     4081 atcccgtatt cacttaataa tttggagtaa atttccatgc cacttttata acgcaataaa
     4141 atcatgaaat cacggtattc tagctcatat ttttggcccg atttaaattc ttcctcagtc
     4201 cgtaatattt ttaccttatt atcaatccag cttctaatca gcatggcaat ggctcgggca
     4261 tcattttcca tttctttttt cttatttctt tccttggcaa tggtataccg gtaaacacct
     4321 gaaaaatacc ctttttcatc tgctctggta gtttgcatag aagaataact tacctgatac
     4381 tcactctcct cattaccaaa tagttcacga aaaagtggat tgacaaattg accgatggag
     4441 tgaagggaac gaaaat
//
LOCUS       DAOQWT010000059         4444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_20031_length_4444_cov_2.798587, whole genome shotgun sequence.
ACCESSION   DAOQWT010000059 DAOQWT010000000
VERSION     DAOQWT010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20031_length_4444_cov_2.798587"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..528)
                     /locus_tag="PKJ95_01965"
     CDS             complement(193..528)
                     /locus_tag="PKJ95_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65040.1"
                     /translation="MNIPSEILREVTGSIFTFPEEEEGNIHLFAENITYKIDEFSSIG
                     ITALYNSSAGSAILMPVYSNQLSSNSKIRIQGGILIESLQQTEDNSLKEVFSKPAYIF
                     LEIGLSYNF"
     gene            complement(610..1278)
                     /locus_tag="PKJ95_01970"
     CDS             complement(610..1278)
                     /locus_tag="PKJ95_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65041.1"
                     /translation="MKKFNFIVTMLIFYLFTLSFTGEASVMTSGGQFNTSLSAFYSKQ
                     NGFILLPHANLDLELTLSLKDNEQVRCDGYFFTDSVEGKLDFFWKKLYWMHRSENFHL
                     SIGKQPISWSFGSLLNPVDYTLGAVALDRDYSPKYQNALEIYFPVNWNASLSLVTSPS
                     GVDRTWKAGLRGRTLIKGFDITANCVQEDIRAGEAGEYRLGITAKGDLGPFGVYGALG
                     YYSD"
     gene            complement(1515..1691)
                     /gene="rpoZ"
                     /locus_tag="PKJ95_01975"
     CDS             complement(1515..1691)
                     /gene="rpoZ"
                     /locus_tag="PKJ95_01975"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNR65042.1"
                     /translation="MEKNDYLDNKYLLTMMVAKRAKQLNQGSKPLVNTNEKNTRVTAL
                     KEIVEGKVYIKEEE"
     gene            complement(1752..1991)
                     /locus_tag="PKJ95_01980"
     CDS             complement(1752..1991)
                     /locus_tag="PKJ95_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65043.1"
                     /translation="MIVKRNEKKHYYSYGDDTHSIKDLIEEKRKEIREIESRFKKLPK
                     NDDMLDNEKEETERDRLKRKIKHLEKEIRDLQRNL"
     gene            complement(2310..2963)
                     /gene="elbB"
                     /locus_tag="PKJ95_01985"
     CDS             complement(2310..2963)
                     /gene="elbB"
                     /locus_tag="PKJ95_01985"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HNR65044.1"
                     /translation="MKTIAVVLAGCGAQDGSEIQEATLTLYFLDKLGANIVCFAPKKP
                     QWHTIDHSTGKVQEEEHRQILQESARICRGEIKDLSQAEVNSLDGLIFPGGFGVAKNL
                     CTYAFEKENCQVDKGVESIILSMHKAGKPQGFICIAPVLAAKVLGKYHPQLTIGNDPS
                     TAKILEQWGAKHVISRVDEMVYDPQQKIASTAAYMLGPTISKIAIGIEKLVIQIMEA"
     gene            3397..3963
                     /locus_tag="PKJ95_01990"
     CDS             3397..3963
                     /locus_tag="PKJ95_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNR65045.1"
                     /translation="MLDTARRYFFTQGYSKATMDELAAELHMSKKTLYQFFKSKQLLL
                     ESVIYDFFQNFHTKINKIIKNKDNNKGVSLLKQVMSLVQAQVSQCNIWAFEDIQKNNP
                     KAWQIINRLEEKLINGELKKLLQEGRKEGTIHSNIDLDLIVLMILNNIKSIVVPEVVS
                     QLSYSTEEVIDMLARIILYGISKPENTE"
     gene            4070..>4444
                     /locus_tag="PKJ95_01995"
     CDS             4070..>4444
                     /locus_tag="PKJ95_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65046.1"
                     /translation="MNRLAQFINKYAKIIIVIVLILTITGATQIKNLRVDDDITKYLA
                     EDSPEILFYQEISKKFEKYDQDLILVSLEYQDLFTLENLQNFKLILEQLEKSDYILSV
                     NSFLNMPKIITTDYGIEIRDFVE"
BASE COUNT         1469 a          802 c          650 g         1523 t
ORIGIN      
        1 gggataaggc agttgcaggc caggcggggg tgcccctttc atctgatcag ctacacccat
       61 attctggtat tttgttttga taataaattc ttccccagct gatttcataa aatcatatcc
      121 tttctaaaag aattataata tttgtaaaaa tattctctca atactattct cattcttata
      181 aattttatta ttttagaaat tatagctaag accaatttcc agaaagatat aggctggttt
      241 gctaaaaact tctttcagtg aattatcttc agtctgctgc aatgattcta ttagtatacc
      301 tccctgaatt ctaattttgc tgttgctact caactgatta ctatagaccg gcattaaaat
      361 tgccgaacct gcactgctgt tgtatagagc agttatacca atacttgaaa actcatcaat
      421 tttataggta atgttttctg caaaaaggtg aatattccct tcttcttctt ctggaaaggt
      481 aaagatagaa ccggttacct ctcttaatat ctcagaaggt atattcaaat attctgcttg
      541 aatataaagc tgattgccag tgggggaaaa ataactataa tcaaaccctg ctaaaaggga
      601 aaatgctctt caatcagaat agtaccctaa agcaccgtat actccaaaag ggcccagatc
      661 tcctttagca gtaataccaa ggcgatattc acctgcttct cctgccctta tatcttcctg
      721 aacacagttt gctgttatat caaaaccttt aattaatgtt ctccccctta aacctgcttt
      781 ccaggtccta tccactccag agggagaggt aaccagcgat aaactagcat tccaattaac
      841 agggaaatat atttccagtg cattttgata ttttggacta taatctctat ctaaggcaac
      901 tgcccctaaa gtataatcaa ccggatttaa tagtgaacca aaagaccagg aaataggttg
      961 cttcccaata gaaagatgaa aattttccga tctatgcatc cagtacaatt tcttccaaaa
     1021 aaaatctaat tttccttcta ctgaatcagt aaagaagtag ccgtcgcatc tgacctgttc
     1081 attgtctttt aaagacaggg ttaactccag atccaaattt gcatgaggca gaagaataaa
     1141 cccgttttgt tttgaataga aagcagacag ggaagtgtta aactgccctc ccgatgtcat
     1201 cacgcttgct tccccagtaa agctcaaggt aaagagataa aaaatcagca tggtaacaat
     1261 aaaattgaat ttcttcattt tcttgccccg gttattaaaa tgattttcta tccagtgagc
     1321 aggttcccct caaaacagtc aaacccattt gtctcacagt ttatatctat tttattatta
     1381 cactttccca atgttattta aagtagtaga cattatttta cattgatatt atataaataa
     1441 aggctgagcc catcctcttt tctctcaaca gaataggctc agcacattta aaaattgttc
     1501 tattaaactt taccctactc ttcttctttt atataaactt ttccttcaac gatttctttt
     1561 aatgccgtta cccttgtgtt tttctcgttg gtgttgacaa gtggttttga accttgattc
     1621 aattgttttg ctcttttggc aaccatcatg gtcagcaaat atttattatc caaataatca
     1681 ttcttttcca atatcttgct cctactggta ttttatttat gtatgaatat aaataaatac
     1741 aaatatttaa attataaatt acgttgtaaa tctctgattt ctttttctaa atgtttgatt
     1801 ttccttttca acctgtctct ttctgtttct tctttctcat tatccagcat atcatcattt
     1861 ttaggtaatt ttttaaaacg tgattcaatt tctctaattt cttttctctt ttcctcaatt
     1921 aaatctttaa tagaatgagt atcgtcccca taagaataat aatgtttttt ttcattcctt
     1981 ttaacgatca cagaacttct ccttacaacc tttctttttt tatatttcaa ttcataaagg
     2041 ggttacccct tttagaaccc tttaaataca gtgataagtg atgagtaatt agtaatgcgt
     2101 tatttgtatt gagttaaaat atgtatatcg tatatatgta atagtattat aataaaaata
     2161 ccctccttcg tcattgggaa cggtttcccc tccttcattt ttttctattt taataaaaat
     2221 taaaaactat ataatattat taattttttt atgaaatact tcttttgata atggaaaggg
     2281 acaaaaaata taataactat gtggtgctat taagcctcca taatctgaat taccagtttt
     2341 tcaataccaa tggcaatttt agatatagtc ggtcctaaca tataggcagc agtagaagct
     2401 attttttgtt gggggtcata taccatttca tccactctgg aaattacatg ctttgctccc
     2461 cattgttcta aaatcttagc agttgacggg tcatttccta tagtcagctg aggatgatat
     2521 ttgcctaaaa ccttagccgc taaaacagga gcaatacaga taaaaccctg tggtttgcca
     2581 gctttgtgca ttgacagaat aatgctctct acccctttat cgacctggca attttctttt
     2641 tcaaaggcat aagtgcacag gttttttgct actccaaatc ccccgggaaa tatcagtcca
     2701 tccagtgagt taacttctgc ttgtgataaa tctttgattt cacctctgca tatacgggct
     2761 gattcttgca gaatttgccg atgctcttct tcctgtactt tcccggtaga atgatcaatg
     2821 gtatgccatt gcggtttttt aggagcaaaa cagacaatgt tggcacctaa cttatccaga
     2881 aaatatagag ttaatgtggc ttcctggatt tcgctaccat cctgagcccc acagccagcc
     2941 aggacaacag caatagtttt cataacagtg actcctttaa ctataaatag ctaaaattca
     3001 aatctattat atattaattg taattaaaac tcaaattttt ataaaactaa atacggatat
     3061 ttttaaacat tgtttagttc atattcgcat tcttatgtca ggtatcggga taagaacaat
     3121 ttttttaaaa ggtttatcaa aaaaaatgaa aaaattatat tgaattaatt attgacatat
     3181 gcagaattat aaattagtat ctggaaggaa actatttata ccattttagt ttttatattt
     3241 ttcattatta caacttttat taccaatatt atacttgagg caatatttct ttataagcac
     3301 aagaatataa gaaagggtat tactattaac gcgaagcagg ctgtatccac aaaaaatgaa
     3361 aaaataaacc aaaaaaacaa cctcattaaa aaaagaatat tagacactgc cagaagatat
     3421 ttctttaccc agggatattc aaaagcaact atggatgaat tggctgctga gcttcacatg
     3481 agcaaaaaaa ccctctatca gttttttaaa agcaagcagc ttttactgga atctgttata
     3541 tatgattttt tccagaattt tcatactaaa attaataaaa taattaaaaa caaagataat
     3601 aataaaggtg taagtctact caaacaggtt atgtccttag tgcaagccca ggtttctcag
     3661 tgtaatatat gggcttttga agacattcaa aagaataacc ccaaagcctg gcaaataatc
     3721 aacagactgg aggaaaaact gattaatgga gaattaaaga agctgcttca agaagggagg
     3781 aaagaaggaa ccattcactc taatattgat cttgacctca tagttctgat gattctcaat
     3841 aacatcaaat ctatagttgt tcccgaagta gtatcacagc tatcttattc tactgaagaa
     3901 gttatcgata tgctggctag aattatcctt tatggtattt ctaagccgga aaatacggaa
     3961 taattttaaa ttcaggatat aatcgttttt aaaattgtaa aacaatagta cgacccataa
     4021 gcttttattt agattataag aatatagaac aggagaatgc agacacagaa tgaacagatt
     4081 agctcagttt attaacaaat acgccaaaat tatcattgta atagtattaa tattgactat
     4141 cacgggggca actcagatta aaaacttaag agtagatgat gatattacca aataccttgc
     4201 tgaagatagt ccagaaatac ttttttacca ggaaatttct aaaaaatttg aaaaatatga
     4261 tcaggacctt atcttagttt ccttagaata tcaggattta ttcaccctgg aaaacctcca
     4321 gaattttaaa ctaattcttg aacagttaga aaaatctgat tatattctct ccgtaaattc
     4381 attcttaaat atgcctaaaa tcattactac tgattatggt attgagataa gagattttgt
     4441 ggag
//
LOCUS       DAOQWT010000060         4440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_20059_length_4440_cov_3.108324, whole genome shotgun sequence.
ACCESSION   DAOQWT010000060 DAOQWT010000000
VERSION     DAOQWT010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20059_length_4440_cov_3.108324"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1655)
                     /locus_tag="PKJ95_02000"
     CDS             complement(177..1655)
                     /locus_tag="PKJ95_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005978438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HNR65047.1"
                     /translation="MIFKNKKDIYQCRNEENLDLNSKNSDGKIFIGISSTGIYCRPIC
                     HARQPKAENRNYFNTAAEAEKAGFRPCLLCRPELAPKIISLNSATSLASQIARYIEEN
                     CGNAITEKDLAQYFNCTEQYLEQQFKENYNVTTSEYLKTYRLLLAKQLLTDTNLSSDE
                     IAKAVGFNNSQNLNKIFKEQYRLEPERLRRKKTQNKEKSDYITLSLAYRAPYRWETLL
                     DFLALRAIPGVEMVKDEEYFRTVHMLNKHGEPVYGWLKVSNRPEKNSLSISISNNLVS
                     VIPKVLSRVRHLFDLSSDPDEVQGKLSSMNQIQPDLFSPGIRIPGAFNAFEMVVRAVL
                     GQQITVKAARTLVDRFAQTFGILLNIGVEGLTHTFPVPDTILSLGDNVADYLGPIGIT
                     SRRAKTILELARAFKYKTIELNFPTKPEKEIEKLKEIPGIGEWTAQYIAMRAMRWPDA
                     FPHTDYGIKKALSPRTDKEILELAESWRPCRSYAAINLWNSL"
     gene            1937..2557
                     /locus_tag="PKJ95_02005"
     CDS             1937..2557
                     /locus_tag="PKJ95_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNR65048.1"
                     /translation="MGDIDFAIIIPFALIAIYTPGPANITSTSLGVRYGIRGAANFLY
                     GSGVGFTFTSLMGGLFSNLLLTVIPSMESVMRLVGASYILYLAYKMLKVDYTFSHNEE
                     VNSHEFGFRYGFVIQLLNVKSIIFIITLYTTFLNPLIGKPFLIFLSAIILGIVGMGAN
                     FLWASLGAMISSILGQERIKKIVNLLLALLLAYTAVKLSGIFEFFW"
     gene            complement(2597..4318)
                     /locus_tag="PKJ95_02010"
     CDS             complement(2597..4318)
                     /locus_tag="PKJ95_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65049.1"
                     /translation="MHYYKPYKGLLLLDLTCATIIAVVDLIFPAVTRYFINYVIPENQ
                     IRTFYYVITVLVILFIIHAIANYIVTYWGHAMGTRMERDMRTDLFKHLHTLSVDYFDN
                     VKTGHIMSRVINDLNHVSELAHHGPEDLFLSFIMIIGSFIILFTIEWRLTLIIYSSLI
                     FLTTYAVFKRRKMSEVYRQVRIKIADVNAQLENSISGIRVAKLFTNEEFEMEKFDVRN
                     REFNKSREMAYKVMAEFYSTIIFMANFLNLLALGLGGYFVFKKILNIGDLVAYILYIN
                     FFLQPIRRLTNFTEQLQEGMSGFERFIELMNIKPSIKDRENAIVLQNVQGKIEFINAS
                     FRYKKEEAVLSNINLEVEPGKTLALVGPSGAGKTTICHLILRFYDVDSGAILIDGINI
                     KDISLHSLRENIGLVQQEVFLFTGTIGENILYGKPDSTWEEVIKAAKNANIHDFIEEL
                     PDGYNTYIGEKGLQLSGGQRQRISLARAFLKNPPILILDEATSSLDTESESIIQDSLE
                     KLTKGRTVLVIAHRLSTIRNADEIIVLTNRGIVEKGTHEELMENNRLYTKLYKGQFRG
                     YIPDQVA"
BASE COUNT         1358 a          814 c          739 g         1529 t
ORIGIN      
        1 caatatcttc tttcacatta tggacaaaat atttttgccc aatcatccac atgcctgtta
       61 tattatcacc atcactcact atcatatatt caccaactgg tgttaggtaa tgtgttgaat
      121 atagcatgaa tacctccttc tttcggttac gaaaaattta tttaattaat ctttaattac
      181 aaagaattcc aaagatttat ggcggcatag ctgcggcagg gtcgccagct ttctgccagt
      241 tccaatatct ctttgtctgt acggggagaa agtgcctttt ttataccata atcggtatgg
      301 ggaaaagcat caggccatct catcgccctc attgcaatat attgtgcggt ccattcacca
      361 ataccaggta tttcttttaa tttctctatc tctttttctg gtttggtggg aaagtttaac
      421 tctatcgttt tatacttaaa cgcacgggct aattctaaga tggtttttgc tcttcttgaa
      481 gtaatgccga taggtcccag ataatcagca acattatctc ccagggacag aattgtatca
      541 ggtaccggga aggtatgggt cagtccctct actccaatat taagtagtat cccaaaggtt
      601 tgagcaaatc tgtctaccaa tgttctggct gctttcacgg taatctgctg tcccagcact
      661 gcccttacta ccatttcaaa ggcattgaaa gccccgggaa tacggatacc tggagaaaac
      721 agatctggtt gaatctgatt cattgaggat aattttccct gaacttcatc aggatcactg
      781 gataaatcaa atagatgtcg aaccctcgaa agcacctttg gtattactga aacgagatta
      841 ttagaaattg agatagagag ggaattcttt tccggtcggt tgcttacttt taaccagcca
      901 tacactggct cgccatgttt attgagcata tgcactgtac gaaaatattc ttcatctttg
      961 accatctcta cacctgggat tgcacgcagg gctaaaaaat ctaacagtgt ttcccaacga
     1021 taaggggctc tataggctaa agaaagggta atgtaatcac tcttttcctt gttttgagtt
     1081 ttttttcttc gtaaacgctc tggttctaaa cggtactgct ctttaaatat tttattgagg
     1141 ttttgcgagt tgttaaaacc aacggctttt gctatttcgt ctgatgaaag attggtgtct
     1201 gtaagtagtt gtttagctaa gagcaaccgg taagttttca gatattctga tgtggtgaca
     1261 ttatagtttt ctttaaattg ttgttctaaa tattgttctg tacaattaaa atattgtgct
     1321 aaatcttttt ctgtgatagc atttccacaa ttctcttcta tatatcttgc tatctgagaa
     1381 gctaacgaag tggctgaatt aagagaaata attttcggag ctaattccgg acggcatagg
     1441 aggcaaggcc gaaatccagc tttttcagct tctgctgcag tattgaagta gttacgattc
     1501 tctgcctttg gttgtctggc atgacaaata ggtctacaat atattcctgt agaggatata
     1561 ccaataaaaa tcttaccatc tgaatttttg ctattcaaat ccaggttttc ttcatttcta
     1621 cattgataga tatctttttt atttttaaat atcacaacaa actccctata ttgttacaat
     1681 ataacattat ttaaaaatat aataattctt ctttaattat ccggtccagt ctatttttta
     1741 attttttatt gcaataattt taaaaaataa agttaacttt tcagcttttt tcatagcact
     1801 attactgcta ttcaatatat tgatgtatat atactgagat actgtattaa catggctatt
     1861 gacatctaca atacaatatt atagattatg tgtattattt tattaataac aatttagata
     1921 aagaaaagga attaaaatgg gagatattga ttttgctatc attatccctt ttgcattaat
     1981 cgctatctat acacccggac cggcaaatat aaccagcaca tcactgggag tcaggtatgg
     2041 tataagaggt gccgctaatt ttttatatgg cagtggtgtt ggcttcacat ttacttcatt
     2101 gatgggtgga ctattttcaa atcttctact aactgttatc ccttccatgg aatctgtaat
     2161 gaggctggta ggcgcttctt atatcttata tttagcctac aaaatgctga aggtcgatta
     2221 cacattttcc cataatgaag aggtcaattc acatgaattt ggttttagat atggatttgt
     2281 aattcagctc ttaaatgtta aaagcattat ttttatcata actttataca ccacttttct
     2341 taaccctttg atcgggaaac cttttcttat atttttatct gctattattt taggaatagt
     2401 tggcatgggg gcgaattttc tatgggcttc cctgggagcg atgattagca gtattttagg
     2461 tcaggaacga ataaagaaaa tcgttaattt acttctggca cttcttttag catatactgc
     2521 tgtaaaatta tcaggcatat tcgaattttt ctggtaatgt agactcgtag ctggaaacaa
     2581 gcaataacca taaacatcag gctacttgat cgggtatata ccctctaaac tgtcctttat
     2641 acaacttggt atatagtcta ttattttcca tcaattcttc atgggtacct ttttctacaa
     2701 ttccacgatt agtaagcacg attatctcat cagcgtttct aatggtggaa agccgatgag
     2761 caatgaccaa taccgttctt cctttggtaa gcttttctaa agaatcttga ataatagatt
     2821 ctgattcggt gtccaatgaa gatgttgctt catctaaaat caagatgggc gggtttttca
     2881 aaaatgcacg ggctaaagaa atcctctgtc tctgaccgcc agataattga agtccttttt
     2941 caccgatata tgtattataa ccatctggca gttcttcaat aaaatcatgt atattagcat
     3001 ttttagctgc tttgataacc tcttcccagg tagaatctgg cttaccataa agtatatttt
     3061 caccaatagt accagtaaac aaaaatacct cctgctgtac cagcccaata ttttcccgta
     3121 aggaatgtaa ggagatatct ttgatattga ttccatcaat tagaatcgca ccactatcga
     3181 catcataaaa acgtaaaatt aaatgacaga tagttgtttt tcctgcacct gaaggtccta
     3241 ccaaagccag ggtttttcct ggctcaacct ctagattaat attggagagt accgcttcct
     3301 cttttttata acgaaaagag gcgtttatga attcaatttt tccttggaca ttttgtaaaa
     3361 ctattgcatt ctccctatcc tttatacttg gcttaatatt cattaactca atgaaccttt
     3421 caaaaccact catcccttcc tgaagctgtt cagtaaaatt ggttagtctg cgaattggtt
     3481 gcaagaaaaa gtttatatat aaaatgtaag ccactaaatc accgatattg agtatttttt
     3541 taaatacaaa atatccacct aaacctaaag ccagtaaatt taggaaatta gccatgaata
     3601 tgatggtaga ataaaattca gccataacct tgtaggccat ctctcgcgat ttattaaatt
     3661 ctcgatttct tacatcaaat ttttccatct cgaattcttc attggtaaac aatttggcta
     3721 ctcttatacc cgagatacta ttttccagct gtgcattaac atcagcaatc ttaatacgaa
     3781 cctggcgata tacttcagac atctttcttc tcttaaaaac agcataggta gttaaaaaaa
     3841 tcaaagagct ataaataatt agggtgagcc gccattcaat ggtgaaaagg attataaatg
     3901 agccaattat cataataaat gataaaaata gatcttcggg accatggtga gcaagctctg
     3961 aaacatgatt aaggtcattt atcactcgtg acatgatgtg ccctgttttt acattatcaa
     4021 aatagtcaac agaaagggtg tgcagatgtt taaagagatc ggtacgcatg tctctctcca
     4081 tcctggtacc catggcatgt ccccaataag taactatata gttagctatg gcatgaataa
     4141 taaaaaggat tacaagaaca gtgataacat aataaaaagt tctgatttga ttttctggta
     4201 ttacataatt gataaaataa cgggtaacag ctggaaatat taaatcaaca actgcaatta
     4261 tagtggcaca ggtcaggtct aataataata gccctttata gggcttgtaa tagtgaataa
     4321 atctttaaaa catgtcaggg gacggtcctt taacatcttt tattttattt aaaattaata
     4381 ttcttaaaac aaacttatca acaacttatc caagcagaat tttttcttta ttttaattta
//
LOCUS       DAOQWT010000061         4411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_20234_length_4411_cov_3.378788, whole genome shotgun sequence.
ACCESSION   DAOQWT010000061 DAOQWT010000000
VERSION     DAOQWT010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20234_length_4411_cov_3.378788"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..853
                     /locus_tag="PKJ95_02015"
     CDS             5..853
                     /locus_tag="PKJ95_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNR65050.1"
                     /translation="MYRINRKIKLYQDMYSMWIEAPEIAKTVQPGQFIILMTKEKGER
                     IPLTVADYDRKEGLVHIVFQVVGKTTDELSCFQEGDCLYSFTGPLGRKSEIDFYGTVV
                     VVGGGTGIACIHPIARSLKEAGNKVIAIIGARSKELIIMEKELKVVSDELIITTDDGS
                     YGRKGFVTHALKEKLDQDPSIKKIWTIGPPIMMKVTTELTKLYPVDTIVSLNTIMVDG
                     TGMCGSCRVSIGEETKFVCTDGPEFDGQLVNWDEFMHRIYRYREEEKESWQDFKNKNE
                     CRCKEN"
     gene            855..2291
                     /gene="gltA"
                     /locus_tag="PKJ95_02020"
     CDS             855..2291
                     /gene="gltA"
                     /locus_tag="PKJ95_02020"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNR65051.1"
                     /translation="MAEMIEMKERTKRETVQMKEQPPSERIHNFKEVPLGYTKEEAIY
                     EAQRCLQCKNPTCMRGCPAEINIPAFIRYIVEEDFAKAYLTILETDALPAVTGRVCPQ
                     EEQCQKECVLNKRGKPIAIGRLERFVADWARENGIHGIKDKRSRKTMKVAIVGSGPAG
                     LTCAADLAKTGYQVTLFEALHKPGGVLVYGIPEFRLPKSIVEYEVDEIEQLGVKLICN
                     QVIGATKSIEDLKDEFDAIFLSNGAGAPRFMDIPGENMNDVYSANEFLTRSNLMKAYL
                     FPEHDTPIKIRQRAATIGAGNVAMDSARTALRLGAEKSYIIYRRSRAEVPARAEEVHH
                     AEEEGIIFHFLTLPVRILGDKEGRVTGMECIKMELGEPDQSGRRSPIPIENSNFVLEV
                     DMVIDAIGTMANPIVARSAEGVEVNQWGYFKIDQRTKMTTCEGIFAGGDIVRGSATVI
                     SAVGDGKIAARSIDDYLSSGKSLKKLII"
     gene            2329..3639
                     /locus_tag="PKJ95_02025"
     CDS             2329..3639
                     /locus_tag="PKJ95_02025"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNR65052.1"
                     /translation="MSQDKELNPFEIVQAQFDENARKGNLDKSMQEFLREAEREIQVR
                     IPVEMDDGTTKTFTGFRVQHSTARGPAKGGLRFAANETIDMVRALAIMMTWKCAVVNI
                     PLGGGKGGVACDPRELSERETEKLCRGWVRKVYDFVGPDMDVPAPDVATNPQDMLWIM
                     DEYDTIARSRKPGFVTGKAVDVGGSLGRTEATGYGVIYCVREAFKRINIDFKGATASI
                     QGAGNNAQYCCEKFTEYGGKVVAMSCWDSNDKKAYTYSCEEGIKPEDLFSPGTLDKFG
                     TINLEKAKEFGWKQEDGDAWIKKPVTVLIPAALGSAVHKDNVNDIKFDVVKVYAEGAN
                     SPTTPEADKIIKEKGIYLIPDFLCNAGGVTVSYFEQVQNNMNYYWPKEEVFEKLNHIM
                     TSAFHDVMDLAEKEKLYARDAAYVIAIQRVARAVEGRGWVKTRK"
     gene            3710..>4411
                     /locus_tag="PKJ95_02030"
     CDS             3710..>4411
                     /locus_tag="PKJ95_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65053.1"
                     /translation="MRVFALIGESGTGKSHKALLIAHKENIEYIIDDGLLIKKDKILA
                     GLSAKKELNKIQAIRRAIFFDKAHAQSVKKIIKEEKPKNIMVLGTSVEMVEKIIETLE
                     LPPVSIVFNIEDISSEKEIQLARTSRFKEGTHVIPLPGIEIQRKFPINLVESLEIFYR
                     KRYSNRKIGERTVVRPPFSYFGKLFISENAILDIIYFQIKDFKDVIRIGKTKININQE
                     GISISINLTLKYGVNI"
BASE COUNT         1513 a          622 c         1032 g         1244 t
ORIGIN      
        1 atagatgtat cgaattaata ggaaaataaa attgtatcag gatatgtaca gtatgtggat
       61 agaagcacct gaaatagcaa aaacagtaca gccgggacag tttattatat tgatgactaa
      121 agaaaaaggg gaaagaatcc cactgactgt tgctgattat gaccgaaagg aaggattggt
      181 gcatattgtt tttcaagtgg ttggtaaaac aacggacgag ttatcctgtt ttcaggaagg
      241 agattgttta tattctttta caggaccttt aggacgtaaa tcagaaatag atttttatgg
      301 tacagtggtg gtagtaggtg gtggaacagg tattgcttgt attcatccta tagcacgtag
      361 tttgaaggaa gctggtaaca aggttatcgc tataattgga gccagaagta aagaactaat
      421 cattatggaa aaggaattaa aagtggtgtc agatgagctg atcataacta cggatgatgg
      481 tagttatggg agaaagggat ttgtcacaca tgctttaaaa gaaaagcttg atcaggaccc
      541 ttctatcaaa aaaatatgga ccattggacc gccaattatg atgaaagtaa ctactgaact
      601 aactaaattg tatcccgttg ataccattgt tagtcttaat actattatgg tcgatggcac
      661 aggaatgtgt ggctcttgta gagtatccat aggtgaggaa acaaaatttg tctgtaccga
      721 tgggcctgaa tttgatggac aattagttaa ttgggacgaa tttatgcatc gaatctatcg
      781 ctatcgggaa gaagaaaaag aatcctggca ggattttaaa aataaaaacg aatgcagatg
      841 taaggagaac tgaaatggct gaaatgattg aaatgaaaga gagaacaaag agagaaacag
      901 ttcaaatgaa ggaacagcca ccatctgaac gcattcacaa tttcaaagag gttcctctgg
      961 gatacactaa agaagaagca atttatgaag cgcagagatg cttacagtgt aaaaatccaa
     1021 cctgtatgag aggatgtccg gcagagataa atatcccagc ctttatccgt tatatagttg
     1081 aagaggattt tgcaaaggca tatctgacta ttttagaaac agatgcttta ccagcagtga
     1141 ctggaagggt ttgtccccag gaagaacaat gtcaaaaaga atgtgttttg aacaaacgag
     1201 gaaaacctat agcaattggt aggttagaac gttttgtagc tgattgggct agggaaaatg
     1261 ggatacacgg aataaaggat aaaagaagta ggaagacaat gaaagttgca attgtgggtt
     1321 ctggacctgc aggattaacc tgtgcagcag atctggcaaa aacgggatat caggttactc
     1381 tttttgaagc attgcataaa cctggagggg ttcttgttta tggaattcca gaattcaggc
     1441 tgcctaagtc gattgttgaa tatgaagttg atgagataga gcaattggga gtgaagttga
     1501 tttgtaatca agtaattggg gcaactaaaa gcatagaaga tttaaaagat gaatttgatg
     1561 ccatatttct ttccaatgga gcaggagcac cccgttttat ggatattccg ggagaaaata
     1621 tgaatgacgt atactctgcc aatgaatttt taacccgttc aaatttaatg aaagcgtacc
     1681 tttttccaga acatgacact ccaataaaaa taaggcagag ggctgccact atcggagcag
     1741 gcaatgtggc aatggattca gctcgaactg ccctgcgcct cggtgctgag aaatcatata
     1801 taatatatcg gcgttccaga gcagaagttc cagccagagc agaagaagtt caccatgctg
     1861 aagaagaggg gattattttt cattttctta ccttacctgt tcggatattg ggggataaag
     1921 aggggagagt aaccgggatg gagtgcataa aaatggagtt gggtgaaccg gaccaatctg
     1981 gcaggcgaag ccccattcca attgagaatt ctaactttgt tttagaagta gatatggtga
     2041 ttgatgccat cggtactatg gctaatccaa ttgttgccag aagtgctgaa ggtgtagaag
     2101 ttaatcaatg gggttatttt aagattgacc aaagaaccaa aatgactacc tgcgaaggaa
     2161 tttttgcggg aggtgatata gtaagaggtt cagcaacagt tatatctgcg gtaggcgatg
     2221 gtaaaatagc tgctcgttca attgatgatt atttatcttc agggaaaagc ttaaaaaaat
     2281 taattattta aaataaaata aaaatataaa atacgggagg tttttaatat gtctcaggat
     2341 aaagaattaa atccttttga aattgttcag gctcagtttg atgagaatgc cagaaaaggt
     2401 aatttagata agtcaatgca ggaattttta agagaagctg agcgagaaat tcaggttaga
     2461 ataccggtag aaatggatga tggaaccacc aaaactttta ccggttttcg tgttcaacat
     2521 tccacagccc gaggtccggc taaaggtggt ttaagatttg ctgcaaatga aactatagat
     2581 atggtaagag ctttagctat tatgatgacc tggaaatgtg ctgtagtcaa tattcctttg
     2641 ggtggaggaa aaggaggtgt ggcttgtgac cccagagaat tgtctgaacg cgaaactgaa
     2701 aaattatgtc ggggctgggt aagaaaagta tatgattttg ttggacccga tatggatgtt
     2761 cctgcgccgg atgttgctac taacccacag gatatgttat ggattatgga tgaatatgat
     2821 actattgcca gaagcagaaa accgggattt gtcaccggga aggctgttga tgtcggtggc
     2881 tctttaggaa gaactgaagc aactggatat ggagttattt attgtgtcag agaagcgttt
     2941 aagagaatca acattgattt taaaggtgct actgcatcaa ttcaaggagc tggtaataat
     3001 gcccagtatt gctgtgaaaa atttactgag tatggtggta aagttgtagc tatgtcctgt
     3061 tgggattcta atgataaaaa ggcatatact tatagttgcg aagagggtat aaagcccgag
     3121 gatttgtttt cccctggaac attagataag tttggtacca ttaatctcga aaaagcaaaa
     3181 gaatttggct ggaaacagga agatggagat gcctggataa aaaagcctgt tactgtttta
     3241 attccagcgg ctttgggttc ggctgtccat aaagacaatg ttaatgacat caaatttgat
     3301 gtagttaagg tttatgctga aggtgcaaac tcgcctacta ctcctgaagc agataagatt
     3361 attaaagaaa aaggtattta tcttattcct gattttttat gtaatgccgg tggtgttact
     3421 gtatcatatt ttgaacaggt tcaaaataat atgaattatt actggcccaa agaagaggta
     3481 tttgaaaaat taaatcatat tatgacttcc gccttccatg atgttatgga tttggctgaa
     3541 aaagaaaaac tgtatgcccg ggatgctgct tatgttattg ctattcaaag agttgcccga
     3601 gcagtggagg gaagaggttg ggtaaaaaca agaaaataat atttagttaa taacgctggt
     3661 taggggattg aatcccttaa ccagaattta tgtggagtat tttaaaaata tgagagtatt
     3721 tgctctgata ggagaaagcg gaactggcaa aagtcacaaa gccctcttga ttgcccataa
     3781 ggaaaatatt gaatatatta ttgatgacgg gctcttaata aaaaaagaca aaattttggc
     3841 aggtctttca gcaaaaaaag aattaaataa gattcaagca attagaagag ctattttttt
     3901 tgataaagct catgcccagt cagttaaaaa aattattaag gaagaaaaac caaaaaatat
     3961 aatggttttg gggacttcag tcgaaatggt ggaaaaaata attgaaaccc tggaattacc
     4021 accagtaagt atagttttta atatagaaga tatttccagc gagaaagaga tacaattagc
     4081 ccggaccagt cgtttcaagg aagggacaca tgttatacct ttgccgggaa ttgaaataca
     4141 gaggaagttt cctattaatt tggtagagtc attagaaata ttttatcgaa aaagatatag
     4201 taacagaaaa atcggagagc gaacggttgt acggccacca tttagttatt ttggaaaact
     4261 ctttatttca gaaaatgcta ttttggatat aatctatttc caaattaaag atttcaaaga
     4321 tgttatcagg attggaaaga caaaaataaa tattaaccaa gagggcattt ccattagtat
     4381 caatctcact ttaaaatatg gtgtaaatat a
//
LOCUS       DAOQWT010000062         4378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_20437_length_4378_cov_2.617858, whole genome shotgun sequence.
ACCESSION   DAOQWT010000062 DAOQWT010000000
VERSION     DAOQWT010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20437_length_4378_cov_2.617858"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..439)
                     /locus_tag="PKJ95_02035"
     CDS             complement(59..439)
                     /locus_tag="PKJ95_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNR65054.1"
                     /translation="MDRKIIKTMTAPSAIGPYSQAIKYGNLIFVSGQIAINPSTNEFV
                     NGNIESQTKMVIENIKAILESAGSSLQKVLKVTIFITDMKDFDSINAVYSEYFNSSLP
                     ARACVEVSNLPKGAKVEMEAIASC"
     gene            complement(466..1149)
                     /locus_tag="PKJ95_02040"
     CDS             complement(466..1149)
                     /locus_tag="PKJ95_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNR65055.1"
                     /translation="MNPLLKDFIPVAKGIARAFGKNCEVILHDVQDLEHSIVLIENGH
                     VTGRKVGGPMTDLGLYFLTSDIFNDINYVANYKTESKDGKRLKSTTIFIRDNIKKIVG
                     FLCINFNLESIVNIAKEIDEFCNIENNLNNAENIIKEEREESFSHSLDELMEQLFSKA
                     QQRIGKDITKMQKEDKIEMVRFLQKHGIFLVKDAIDRLAEKLNVSRFTIYNYLAEIKP
                     ENGSGKIIL"
     gene            complement(1721..2416)
                     /locus_tag="PKJ95_02045"
     CDS             complement(1721..2416)
                     /locus_tag="PKJ95_02045"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNR65056.1"
                     /translation="MKITRILFYFYTTVLLSMLLATFTLTATESVSNDIPVFTSNPNI
                     KGKEGMPYTYKAKASCIDNDVLEYYLLIYPEGMNIELSTGLINWVPESPGSYDVIVKA
                     SDIKKSISATQEFTIVVEKALLTSIKVLPSTVMYLDMNESKPIQSVTAYYDNNTSKEI
                     ELANCSYESDHQDIATVDNNGTVTGITNIISIPATITVSYSEENISKNDIVLVVVNPP
                     FIVFRGMDQACAF"
     gene            complement(3056..4192)
                     /locus_tag="PKJ95_02050"
     CDS             complement(3056..4192)
                     /locus_tag="PKJ95_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNR65057.1"
                     /translation="MSNKWLRKTLIILLSVIMMVIAGREFLIWLNEKEPIVEGPGVTR
                     VAKLSDYFADLKGTALDTNIYILEGEEDGGAVMVTAGVHTNELSGVMTNVILIENAHV
                     EKGKVILIPFANNSAFTAGHPGEAYPMRYHIKTDWGSRWFRFGDRYTNPIHQWPDPEV
                     YVHYPSGQSLSGFDIRNLNRTFPGKADGTITEKVGHAIMQVIEKENVDLFIDLHEAEP
                     MYPVINTIVANQKAMDIAVIAEMNLSAFEGIKIGKEISPENLHGLTHREVGDHSRALS
                     LLAETCDPIQDPLRGRTDEALIITGKDEFILKAAKLGLLYVPFDETGSHIDVRTGRHT
                     STVLEIISAFSELYPDKEIIVSDAPRYAEIIEKGVGYFLLKPRG"
BASE COUNT         1461 a          751 c          593 g         1573 t
ORIGIN      
        1 caccatacca atttttaacc aaacagtatt aaattaaaaa ataatacctt caaatccatt
       61 agcagctagc aatagcttcc atttcaactt tagctccttt tggtaaattg gaaacctcca
      121 cacaagcacg cgctggcaaa ctggagttaa agtattctga ataaacagca ttaatgctat
      181 caaaatcttt catatcagta ataaagatag ttactttcaa taccttctgt aatgaacttc
      241 cggcactttc caaaattgct ttgatatttt caattaccat ttttgtttgg ctctcaatat
      301 taccgttaac aaactcattt gttgaggggt taattgcaat ctgtccagaa acaaaaatca
      361 ggttaccata tttaatcgcc tgagaataag ggccgattgc ggagggagca gtcatagttt
      421 tgataatctt tctgtctatg gtaaacacaa cctttcttat ttagattaaa gtattatttt
      481 tccactacca ttttcaggct ttatttcagc aaggtaattg tagattgtaa aacgggaaac
      541 attcaatttt tctgccaacc ggtcaattgc gtctttcacc aaaaaaattc catgtttttg
      601 caagaaccga accatttcta ttttgtcttc tttttgcatt ttagttatgt ctttgccgat
      661 tctctgctga gccttagaaa acaactgttc catcaattca tccagggaat gggagaatga
      721 ttcttctctt tcttctttaa taatattctc agcattattc aaattgtttt caatattaca
      781 aaattcgtct atctctttag ctatatttac aattgattct aaattaaaat taatgcataa
      841 gaagccaaca attttcttaa tgttatcacg aataaaaatt gtggtagatt ttaatctctt
      901 tccatcttta ctttctgttt tataattagc cacataatta atgtcattga agatatcaga
      961 ggttagaaaa tataatccaa ggtcagtcat tggaccccca acttttcggc ctgttacatg
     1021 tccattttca attaatacaa tagaatgttc aagatcttgg acatcatgaa gtataacctc
     1081 acaatttttt ccaaaagcac gagctatgcc ttttgctacg gggataaaat cttttaaaag
     1141 ggggttcatc tttcaccaac tatttcaata tgtaattagt aaaagtgtta ttctttatga
     1201 aagcctattt cttttaaaat agcacaccat aaaataaatg tcaatattat attaaaatat
     1261 ttttgtaaaa tactaaaaat attttaatat cattaaatat aatgttttaa gttattttta
     1321 ggctaaagac ctgacctctt tgacaatttt atattgctaa taatcaatta aatcccgaat
     1381 cagtaatacc tatactcata aatatcttct aatgcgcaaa tattataagc attttattaa
     1441 agtatttcct tcttttaatt acttaagttg ctgaaaattt ttaaggttga attattatga
     1501 ataagtattc taaggttaga gttaatgttg ttgagataac ctctgatttt gctattctaa
     1561 ctgcaaaaca cgatttattg caaaatgcat ggcacaagtt ataaactctt tacaatattg
     1621 gaaaaaacaa tagactggct cttacaacaa atatgataat ttttttttaa tctatttctt
     1681 gtggtggttt accgataacc tatcggtaat aatcgctttg tcagaaagca catgcttgat
     1741 ccatccctct aaatacaata aagggggggt taactaccac taatactata tcattcttag
     1801 aaatattttc ttcagaataa ctaacagtga tagttgcggg tatggaaatg atatttgtaa
     1861 ttcctgtgac tgttccatta ttatcaaccg tggctatatc ttgatgatct gattcgtaac
     1921 tgcaattggc aagttctatt tctttgctag tattattatc ataataagca gtaactgatt
     1981 gaatgggttt tgattcattc atatcaagat acattacagt agaaggaaga acctttatag
     2041 aagtaagcaa cgctttttca actacaattg taaactcttg agtagcagat attgatttct
     2101 ttatgtcact tgcttttacg ataacatcat agctcccagg agattcggga acccaattaa
     2161 tcaggccggt ggataattca atgttcatac cttcaggata aatcaataaa taatactcta
     2221 aaacatcatt atcaatacaa ctagcctttg ccttataagt gtagggcatc ccctctttac
     2281 cctttatatt tggattagat gtaaatacag gtatatcatt tgaaacactt tctgttgctg
     2341 taagtgtaaa agtagctagc agcatagata aaagaactgt tgtataaaaa taaaatagta
     2401 ttcgtgttat tttcataaaa atctcctttt tttctatatt tgttgtacaa ttatacaaaa
     2461 aattttttat gattttaatc aaaaaatatt taatggaaat gtattaagtt ttaaaatatg
     2521 tcttagctta agaaatagta cttattggaa ttccattatt taacaaatct ttacatataa
     2581 agaaagatat agggtaaata ttataatgcc ctggtatttt gtatttaaat aaattttcaa
     2641 ctggtaaact aatctttact ttacagtcaa ttattgaaag gaatctgtta tgcttataaa
     2701 ttataatgtt tattcaaata taagtagaat aatagtcttt tattaaatga ttagtaataa
     2761 agtctaaaaa ttgtcaatta attcttcact ggcttgcatt ctaaaataaa aaaatggggc
     2821 agatactaac aattggtcta ccccttccta tgaattctac aatttatgtt ttaaataaat
     2881 attatttcag cctgtaaaat ttacaaatgg ctaactgttt atgacactaa aatataataa
     2941 taaccactaa ctcataatat ttattgaaag aaggtaaagt aagtgacata ttcttaaaaa
     3001 atattatttt ttaaactgtt ttgattttct taaattttct taaatgtttc taaaatcaac
     3061 ctctcggttt taataagaaa taaccgacac ctttttcaat tatttcagca tatcttggtg
     3121 catcagaaac aataatttct ttatcaggat ataattcaga aaaagcactt attatttcta
     3181 aaaccgtaga ggtatgtcta ccggtacgta catcaatgtg tgaacctgtt tcgtcaaatg
     3241 gaacatagag taatccaagt tttgccgctt ttaaaataaa ttcgtcttta ccggtaataa
     3301 ttaaagcttc atctgttcga cctcttaatg gatcctgaat tgggtcgcag gtttcagcca
     3361 gcaatgataa tgcccttgaa tgatctccaa cctctctatg ggtaagtcca tgtaaattct
     3421 caggagatat ttctttccct atttttattc cttcaaaagc actcaaattc atctctgcga
     3481 taactgctat atccattgct ttttgatttg ccactatagt attaataaca ggatacattg
     3541 gctctgcctc gtgaagatct ataaataaat ctacattttc tttttcaata acctgcataa
     3601 ttgcatgccc aactttttcg gttatagttc catcagcttt tcctggaaaa gtacgattaa
     3661 ggttcctgat atcaaaacca gaaagtgatt gtccgctggg ataatgtaca tatacttcag
     3721 ggtcaggcca ttgatgaata gggttagtat agcggtctcc aaacctaaac catctgcttc
     3781 cccaatctgt ttttatgtgg tagcgcatcg gataggcttc tccagggtga ccggcagtaa
     3841 aagcgctatt attggcaaat ggaataagaa tgactttccc tttttcaacg tgagcatttt
     3901 ctattaatat aacatttgtc attacccctg aaagttcatt agtatgaacc cccgctgtaa
     3961 ccataacagc tcccccatct tcttcccctt ctaatatata tatattagta tcaagagctg
     4021 ttccttttaa atcagcaaag taatcactta actttgctac tctggtaacc ccagggccct
     4081 ctactattgg ttctttttcg tttaaccata ttaaaaactc tcttcctgca attaccatca
     4141 taatgacaga taacagaatg ataagagttt ttcttaacca cttatttgac atacttagcc
     4201 ccctttctta tataaattga attcatttct acctctaatt atttaatata ttcgtaaaat
     4261 tctaagtatg aatggcatta atacaaaagc ataaagaatt tcattataca gattctcttc
     4321 ttcaacaaaa ttccataata gtaaaatagc tacaaaagca caaaacaggt aataaata
//
LOCUS       DAOQWT010000063         4338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_20691_length_4338_cov_3.028018, whole genome shotgun sequence.
ACCESSION   DAOQWT010000063 DAOQWT010000000
VERSION     DAOQWT010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20691_length_4338_cov_3.028018"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="PKJ95_02055"
     CDS             <1..1109
                     /locus_tag="PKJ95_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNR65058.1"
                     /translation="RCRNTGIAVTLISLIFISPILHLIGVTPETHGYAHGYLAIIFAG
                     SIIIMLNFLLTLMLRSEGAVKVAMYGMFIGTGINIVLDPIFIQVLGYGVKGAAIATLI
                     GQGVGLLYYFDFYRKKKSMVSLHWEYISLRSDIILEILKVGIPASSYNIMLSIANTIG
                     NYVAAGYNDLILAAYGINRRIFSMAMMTLTGLAEGSQPIIGYSYGARKIDRMNKVIKT
                     SISLAAGISFFFVGFFYLFAGKMIQIFINNEEVISYGIQIMRAMIISLPLMAIQFLIR
                     VTFQALGKGKPAFMLALARDIFYIPALFALNKYFGFSGFVYAQPFAHVFTFLLAIVLY
                     NTIRGKITEEHREWEIKNNLASTEVATSTIGKSN"
     gene            complement(1241..1894)
                     /locus_tag="PKJ95_02060"
     CDS             complement(1241..1894)
                     /locus_tag="PKJ95_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNR65059.1"
                     /translation="MGTLRLFSIIFLTVIAGADLYLIGTSQEKKRIWTKPFIIPLIIF
                     IYSQYAKQINYSLVMALAFSFFGDFFLLFSEKKIFFQTGLFSFLMSHILYIYTFTGTI
                     SLSNVPPIRYLVLIIPYLIYILVFLKILKPYLENYLIPVILYTITIAGMSYASLLRFW
                     TTTGFYFWLPFIGSLFFIISDSLLAIRNFRYGQKKGWITVMLSYILGQLFIVMGFMG"
     gene            2166..2678
                     /locus_tag="PKJ95_02065"
     CDS             2166..2678
                     /locus_tag="PKJ95_02065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNR65060.1"
                     /translation="MDGITALLVIDMQYGLLQRNVFYKEQLIDNVNALLDFFHNREKP
                     VFIIRHTNTSFSAENTDNWQIDSNVKRADSDILLNKTHSSVFKEKRFISLLKENSISD
                     VVVTGLVSNGCVKAACLDARVNGLNVTLISDGHSTFHKEGEDMVSYWNKYLHDEGIQV
                     ISTAEFLKMN"
     gene            complement(2760..3392)
                     /locus_tag="PKJ95_02070"
     CDS             complement(2760..3392)
                     /locus_tag="PKJ95_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNR65061.1"
                     /translation="MPFDFKKEYKKFYRPPRKPVIVEIPEMNFLAVKGKGNPYEEMGE
                     YKVAVGLLYTIAYTIKMSYKGNYKIDGYYQYVVPPLEGLWWQTGTNGIDYKNKDRFHW
                     ISLLRLPDFIAREDFEWAIKEATKKKKDDFSKVDFFTHSEGLCVQCMHIGPYDNEPIT
                     ISRMNDYIEQNGYISDITDKRHHHEIYLSDPRKGDLSKLKTVIREPIKRI"
     gene            complement(3891..4139)
                     /locus_tag="PKJ95_02075"
     CDS             complement(3891..4139)
                     /locus_tag="PKJ95_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010027186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6429 family protein"
                     /protein_id="HNR65062.1"
                     /translation="MEKVIKELTLLLLYLTSWQEKEFSIKIQRSWKGYPFEILDELRE
                     EGFITGSNRAKSVYFTEEGIEKAKEFMNKYRINETGLE"
BASE COUNT         1376 a          820 c          680 g         1462 t
ORIGIN      
        1 atagatgtcg caatacaggt atagcagtca cactcatcag tcttatcttc atctctccaa
       61 ttttacactt aataggagtt actccggaaa cacacggtta tgcccatggc tacctggcaa
      121 taatatttgc tggcagcatt atcataatgt tgaattttct cttaaccctt atgcttcgtt
      181 ctgaaggggc agttaaggtt gctatgtacg gaatgtttat cgggactggt ataaatatag
      241 tattagaccc catctttatc caggtgttgg gttatggagt aaagggagca gctattgcca
      301 cacttatcgg ccagggagtt ggcctgctct actactttga tttttaccgt aagaagaaaa
      361 gcatggtatc tctgcactgg gaatatatct ccttacgaag tgatattata cttgaaattc
      421 taaaggtagg aattccggct tcatcataca atataatgct cagtatcgcc aataccatag
      481 gcaattatgt agcggctggt tataatgacc ttatactggc agcttatggt atcaaccgcc
      541 gtattttttc tatggctatg atgactttaa cgggattagc ggaaggctcc cagcccataa
      601 tcggctatag ctacggtgcc cgtaagatag accgtatgaa taaggttatc aaaacatcca
      661 tttccctcgc cgctggcatc tcttttttct ttgtaggatt tttctacctc tttgccggaa
      721 agatgatcca gatatttata aacaacgaag aggtaatttc ctatggtata cagataatgc
      781 gagctatgat tatttcctta cctcttatgg caattcaatt tttaattagg gttacctttc
      841 aggcgttggg taaaggaaag cctgctttta tgctagcttt ggcacgggat atattttata
      901 tacctgccct ttttgctctg aacaaatatt ttgggttttc tggattcgtt tatgcacagc
      961 catttgccca tgtttttact ttcttgcttg caatcgtctt gtataacacc atcagaggga
     1021 agataaccga agaacataga gaatgggaaa tcaaaaacaa cttagccagc accgaggttg
     1081 ccacaagtac catcggaaag agcaattaaa ataagtgcca acgacttctc atctgcacct
     1141 tgagctattt ttaatatagt tatttcctga ctcattcttt ttttgcatcc tgcattaggt
     1201 tttcctataa caaaaacccg tcagcgtacc ataaacttaa tcaacccata aatcccatta
     1261 cgatgaataa ttgccccaga atatagctta acataaccgt tatccagcct tttttctgcc
     1321 catatctaaa attacggata gctaaaaggg aatctgagat gataaaaaac aatgaaccga
     1381 taaagggtaa ccagaaataa aaaccagtag tggtccaaaa acgtaataaa ctggcgtagc
     1441 tcatcccggc aatagtgatg gtatacaata taaccggaat caggtaattc tccagatacg
     1501 gttttaaaat ttttaaaaaa accaagatat aaatcaggta ggggataatg agaactaaat
     1561 accttatagg aggtacatta ctcaaagata tcgtacctgt aaaggtatag atatataaaa
     1621 tatggctcat taaaaaggag aataatcccg tttggaaaaa aatcttcttt tctgaaaaga
     1681 gcaaaaagaa atcgccgaaa aaagagaaag ccagtgccat aacaagagaa taattaatct
     1741 gtttggcgta ctgactgtag ataaagataa ttagtggaat gatgaatggc ttggtccata
     1801 ttcttttctt ttcctgagaa gttcctatca gataaagatc tgcacctgca atcacagtta
     1861 ggaaaataat tgaaaataat ctaagtgttc ccattttgct cccatcctgt aataatatta
     1921 gtagatatat ctacctatta gttatactga taatattaaa cttctgctga agtattttat
     1981 accaataaaa aacagtcact ctattataca gtacaaatct ttttgatgtt ataattgtaa
     2041 tgattttgaa gtgcacttac aatttaaaaa ataaaagtat tattatgctt acccttaatg
     2101 tagaattatt ttggaataaa acaatttttg tcagatttat aagataaaaa aggagattaa
     2161 tgattatgga tggtattaca gcattactgg taatcgatat gcaatatgga ctgctacaga
     2221 ggaatgtttt ctataaagag caattgatcg ataatgtgaa tgctcttcta gatttttttc
     2281 ataacagaga aaaaccggtc ttcataatca gacataccaa caccagtttt tcggcagaaa
     2341 atacggataa ctggcagata gatagcaatg ttaagagagc agattccgat atcctgttaa
     2401 ataagacaca tagtagtgtc tttaaagaaa aacgatttat atctttgctt aaagaaaaca
     2461 gcatctctga tgttgtagtg acaggtctcg tctcaaatgg ctgtgtaaag gcagcctgtc
     2521 ttgatgccag agtaaatgga cttaatgtaa ccctaattag cgatggacat agtacctttc
     2581 acaaagaggg agaggatatg gtcagctact ggaacaaata tctacacgat gagggaattc
     2641 aggtaatttc aactgctgaa tttttaaaaa tgaattgaaa caatatttta tatattttgt
     2701 gtcttagtat ttaagttgct tatcctgttc aacaatttgt aaaacatcct cgcaaacatt
     2761 tatatacgtt ttatgggttc ccttatgaca gtttttagtt tactcagatc acctttcctt
     2821 ggatcagaaa gataaatttc atggtgatgc cttttatctg ttatatcgct aatgtagcca
     2881 ttctgttcta tataatcatt catacgcgat atagttatag gttcattatc atatggccct
     2941 atatgcatac attgtacaca taagccttca ctatgggtaa aaaaatcaac ctttgaaaaa
     3001 tcatcttttt tctttttggt agcctctttt atggcccatt caaaatcttc cctagcaata
     3061 aaatcgggta aacggagcaa agaaatccag tggaatctat ctttattctt ataatcaatc
     3121 ccattagttc ccgtttgcca ccagagtcct tccagaggag gaactacata ttggtaatag
     3181 ccatctatct tataattccc cttatagctc atctttatag tataggcaat tgtatataat
     3241 aaacccactg ctactttata ttctcccatc tcctcatagg gatttccctt ccctttaact
     3301 gccagaaaat tcatctcagg gatttcaact ataaccggct ttcgcggtgg cctataaaat
     3361 ttcttatact cttttttaaa atcaaaaggc atggcattta cctcttctat tgatatctta
     3421 ttgttataag taactttgat aaatattcca caatatattg ctatttcgga tattattgat
     3481 ttccgccttt tactaaaaac taaaaactac tttcatttat accccagtct ttatctttgt
     3541 cttttactaa aaactataaa ctgaaaaatg tattggtttc cactttcaac tttctgcttt
     3601 ccaccttatt ccctcactta ttcccacatc ttgtttttac taactacttg tacaattcaa
     3661 atatctcatt cacagcaatc tatattttta tctttgcatt tatgccaaaa accatgaact
     3721 attaactata aacctccatc gtttattccc acattggatg aactaaatac catcgaccaa
     3781 cgactatctt cacacaatac gaagtacaca caatgacaac gggtatgtca gatgaaccgt
     3841 ccctccctat gacagacatg ctttgggttt cgccaggctt attaacctta tcattccagg
     3901 cctgtctcat taattctata tttgttcata aattctttag ccttttcgat tccctcttca
     3961 gtaaaatata ctgattttgc tcgattactt ccagtaataa aaccttcttc tcttaattca
     4021 tctaaaatct caaagggata gcccttccag cttctttgaa tttttattga aaattctttt
     4081 tcttgccatg aagtaagata gagtaataat aatgttaatt ctttaatcac tttttccatt
     4141 ttttacctcg ccaaaaaaat atttacattt ttgatttcta taaatatttc atctcatcca
     4201 gaaaagcagg tctatttgca tattttgttt taagcaaaca aattaattta tcgaattctg
     4261 cttcaaaacc tcttattttt ttcatttgtt ttagatatgt aacaattctt ctatagtgcg
     4321 ttcttcccat tgtagaat
//
LOCUS       DAOQWT010000064         4269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_21111_length_4269_cov_2.966303, whole genome shotgun sequence.
ACCESSION   DAOQWT010000064 DAOQWT010000000
VERSION     DAOQWT010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21111_length_4269_cov_2.966303"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PKJ95_02080"
     CDS             complement(<1..526)
                     /locus_tag="PKJ95_02080"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNR65063.1"
                     /translation="MNKQYPSIKFGDFVEIQPIVKINKNTKYSFVPMESIIPGMKYVY
                     PSDEKKYYGSGVKFKNDDIIFARITPCLEHGKIAIARGLKKGKGFGSTEYYIFRAKQN
                     ISDKDYVYYLSISPIIKENAIVSMVGASGRQRAQKDVIGKIIIKPPNYSTQQKISSIL
                     SNYDNLIDNNQRRIE"
     gene            complement(523..2607)
                     /locus_tag="PKJ95_02085"
                     /pseudo
     CDS             complement(523..2607)
                     /locus_tag="PKJ95_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002761597.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(2808..3482)
                     /gene="radC"
                     /locus_tag="PKJ95_02090"
     CDS             complement(2808..3482)
                     /gene="radC"
                     /locus_tag="PKJ95_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNR65064.1"
                     /translation="MAKIKDLPKHKRPREKLSERGAENLTDAELLAILIRTGRAGKSA
                     LDIAKETLKKYPLTRLLSVTQDELHSIGGLEGTKSITIQAALELGRRASAIFDDSLPF
                     IDSVNAALTQLHDIRTKQKEYFVVLYLNARKQLIRKETISIGTLTETLVHPREVFKPA
                     IGCLASSIILAHNHPDNSIDISEADRKITEKLIQSGVILDIEVIDHIIVTNNNYISFK
                     EQDLLF"
     gene            complement(3759..4223)
                     /locus_tag="PKJ95_02095"
     CDS             complement(3759..4223)
                     /locus_tag="PKJ95_02095"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HNR65065.1"
                     /translation="MNIIIAVIIGALEGMLRLPGLIGKIYPYTMALPTISVSVRRLHD
                     TDRSGWCFLLNLIPVLGSIFFFLFTIEDSTPGENKYGPNPKEEGFEIPQVTPADMKKC
                     PQCGELVTREARVCHHCNYEFFQVPKTIEEEIEEFDKQQKQKSPHYSNDEII"
BASE COUNT         1173 a          843 c          732 g         1521 t
ORIGIN      
        1 gctcaatccg cctttggttg ttatcaataa gattgtcata gttcgatagt attgaggaaa
       61 ttttttgttg tgttgaataa tttgggggct taataattat ttttccgatt acatcttttt
      121 gtgccctttg cctgccagaa gcaccaacca tgcttacaat tgcattttct ttaattatag
      181 gtgaaattga tagataataa acataatctt tatctgaaat attttgcttt gctctaaaaa
      241 tataatattc tgttgaacca aatcctttac cttttttaag tccccgtgca attgcaattt
      301 ttccatgttc taaacaaggc gttattctag caaaaataat atcatcattt ttaaatttta
      361 cccctgaccc ataatatttt ttttcatctg atggataaac gtatttcata ccaggtataa
      421 ttgactccat agggacaaaa ctgtatttag tgtttttgtt aattttcact attggttgta
      481 tctcaacaaa atctccaaat tttatacttg gatattgttt attcatattc ccaatttcct
      541 gaaattctca ttaatctttt cagctaattc attttcctga gtatttagct cttctaattc
      601 tttatgaata ttatgcatcg tctctgaaaa atcaaaatcc gggtcttctt cctctggggc
      661 tactcctaca tatctgccgg gggtcaggct ccaatcattt tccttcagtt cttctctgtc
      721 taccagtttt accaatcctt caacatcacg gtatttgcca tcggaaaatc tctctatgag
      781 ccattctgcc tgatgatgaa aatagcggat ttcttttaat tgattgacca gatcatcacg
      841 tgcttcttca acctcttttc taagattctg tatctctttt atattccatt ggctgctatt
      901 tttagcactg agtttgctct ggcaatcatc gataaggcgg tagagcaacc gatagagcat
      961 ctctgcctct ttaatcagct tcttgttctc ttccgaaaaa atatttagtt cctcaagagc
     1021 tacgctgagt tttaaattct cttgaacctg ttttttctac caatcactct tctcttttat
     1081 taattccgta aatagtacag aatcttgctg atacgcttcc cgatatttat tcaattcatc
     1141 tgttatttca ctaatctttt cgtttgcagg catagtcttc aaaaaagatt ctatattttt
     1201 atataaacgg gtgaaggatt tttggtattc ctgaataagg gttaggctct tttctgcttc
     1261 cccgagtgtt tttgatatat agtcggtgac taatcgatgg aagcgttctt cctgatctct
     1321 gtacagccag atgatagaga gaatattctg ctcttgttca gggctaaaat caaagatacg
     1381 gcgggtaacc ttgcggtata tttctcgggc atcgatcatc agtatcttac cctgcaggct
     1441 ttctggttta tttttgtcaa aaaaccagag ttcacaagga acactacggg tataaaagaa
     1501 attggaacgg atagcaatca tgatatccac atgaccggtt tcaattagtt ttcggcgaac
     1561 tttcgcctca acacccccag cactggaagc ctgagaagac attacaaacc cggcacgtcc
     1621 tttctcattc agataactat agaaataact aatccagaca tagtttccat tggacacttt
     1681 gcctttttta tttacaccgg gtagaccaaa aggcaaacga gggtcattct ttattttgtc
     1741 tgcatctatt tcatcaacat tgaagggcgg atttgccatt acaaaatccg ctttatgtaa
     1801 catttcgtga gggtcttcat agtaagttat tgcctttttg atatcaccca ccagaccgtg
     1861 tactgccaga ttcatctttg ccaggttgat ggtggtggca ttcttttcca gtccgaagaa
     1921 gtttaaatta tcagtagggt tttcatgaat cctttccaca aaacgggcac tctgcacaaa
     1981 catgcctcca ctaccgcagg cagggtctaa gatggaacct cttttgggct ccaatacatt
     2041 ggctataaga gatactagag aaaccggggt aaagaattca cctccgtcat gggctttctg
     2101 gtcggcaaac tgggttaaaa aatactcata gattctgcca aagacatcac cagaaacttt
     2161 ctttaattct tccgggttta gcgttcgtag taattgggca attatttcat tatccaactc
     2221 ccgatattcc tgtttaggaa gaacacctcg aagggtttca taatcgcttt caatagattc
     2281 cattgcatcg ataagggctt tggcacggtc atcactatcg gtaagactga ctaaatagtc
     2341 aaactgggca tgtggtgcta aatatatggc tgatttttta ctgaaatctt ctttgctaag
     2401 cgcccttttt acaccgccac gggtgggaag tcctgcctca atctcatctt ttaccgccag
     2461 atatcggcta taggcatggc gtagaaagat taaacccatt accggcataa aatattcatt
     2521 actggcataa tttgaatttg cccgcagggt gtctgctgca ttccagagtc ttttctcaat
     2581 cgcttcaata ttttccagtt gtgccatagc ttagttcccc ttccgttttt cggttatcgt
     2641 tattggtact ggcttacctc aatattttag tttgattttg ttgttagtta attggttaat
     2701 tggttggtta gttaattagc taattcttta tatttttgat ttttgaaata tatatttaaa
     2761 ttgttaaaaa atataattat ttattgacca ttacattttt aaaaagttta aaataacagg
     2821 tcttgctctt taaagctgat ataattattg ttagtcacta tgatatgatc aataacttct
     2881 atatccaaaa taactccaga ttgaattaac ttttctgtta ttttcctatc tgcctctgaa
     2941 atatcgatac tattgtcagg atgattatgt gctaaaataa tcgagctggc taaacagcct
     3001 attgccggct tgaagacttc ccggggatgt actagtgttt cggtgagagt cccgatagag
     3061 atggtctctt ttcttatcaa ctgctttctg gcatttaaat ataagactac aaaatattct
     3121 ttctgtttgg tgcgtatatc atgaagctgg gtaagtgcag catttacaga gtctataaag
     3181 ggtagagaat catcaaaaat agctgatgcc ctccttccaa gctccagcgc tgcctgaatg
     3241 gttatcgatt ttgttccttc taatcccccg atactatgca attcatcctg agtgactgat
     3301 aacagcctgg tcagcggata tttctttaag gtctctttgg caatatccaa tgcactttta
     3361 ccggcacgtc cggtacgtat tagaattgcc agaagttcgg catcagtgag attttccgcc
     3421 cctctttcag acaatttttc tcttggtctt ttgtgttttg gtaagtcttt gattttagcc
     3481 atgtttgaag cttttttatt tttaaccttt acagtacagt tttattcatt ttttatttat
     3541 ttttgtcata tgtaataggt attgttacac ttgtttcctt atagtttcta tcataccaaa
     3601 ataaaattag gaattccatt ttttaaatat ttgcaaataa tgctattcta ttgctttcca
     3661 gaaaaacact ttagcaaacc aaagataaaa atatttgata cataactgta cgataatgcc
     3721 acatctacca gaagtagact gcaaagagga atgtatttct aaattatttc atcattagaa
     3781 tagtggggag atttttgttt ttgttgttta tcaaattctt ctatttcttc ttctattgtt
     3841 ttgggaacct ggaaaaattc ataattacaa tgatggcaga cacgtgcttc tctagttacc
     3901 aattctccac attgtggaca tttcttcata tcggcaggtg ttacctgggg aatttcgaac
     3961 ccctcttctt ttggatttgg accatattta ttttccccgg gagtgctatc ttcaatggta
     4021 aataaaaaga aaaatattga accgagcact ggaattaaat tgagaagaaa gcaccaacca
     4081 cttctatcag tatcatgaag ccgcctgact gaaaccgaga tagtggggag agccatggta
     4141 tagggataga tttttcctat caatcccggc aatctaagca taccctctaa agcacctatt
     4201 attactgcaa taataatgtt catcaaatag aacatcaatt agccgaggtt ggcgtgggtc
     4261 atatcgcca
//
LOCUS       DAOQWT010000065         4266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_21130_length_4266_cov_3.123486, whole genome shotgun sequence.
ACCESSION   DAOQWT010000065 DAOQWT010000000
VERSION     DAOQWT010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21130_length_4266_cov_3.123486"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3039)
                     /gene="nifJ"
                     /locus_tag="PKJ95_02100"
     CDS             complement(<1..3039)
                     /gene="nifJ"
                     /locus_tag="PKJ95_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNR65066.1"
                     /translation="MKHKLVAFDGNTAAAYTAYHISENVTIYPITPSSVMGELADLWA
                     FQGKKNIWGAVPAVAEMQSEAGAAGAVHGSLQRGALTTTFTASQGLLLMLPNMFKISG
                     ELSPVVFNVSARTVATHALSIFGDHSDVMAARTTGFALLAAASVQEAMDFPLIAQAAS
                     LKSRIPFLHFFDGFRTSHEISKVELLEVEEMKRMIDDKLVYEHRARALSPERPFIRGT
                     AQNPDVFFQAREGCNPFYDICADITEETMKEFKEITGREYHLFQYEGATDAEYVFLIM
                     ASGCETVQETVEHLIGKGEKVGMIKVRLFRPFSVRHLYQAIPETAKILCVLDRTKEPG
                     SAGEPLYTEVLSGINEYMSENNIDRKIRVFGGRYGLSSKEFTPAMVMGVYEEIKKENP
                     LNHFTVGINDDVTHRSVSYPEHFHIEPVDRVRAVFYGLGSDGTVSANKSSIKIIGEET
                     DNFAQGYFVYDSRKAGSLTISHLRFGKDPIHSPYLIQRANFVGCHQFSFLDKYDVLKY
                     AEDNAVLLLNSPYPAEEVWDKIPREVQQKIIDKKIKLFTINAYQIARETGLGVRINTV
                     MQTAFFLLSKVLPEDVAIEKIKQFIEKSYGSRGEEILRRNNEAVDLAMKSIKEIKVPA
                     RAEGTIRKKDTVSEKAPQFVREVTARMIAQEGDDIPVSAFQPDGTFPSGTTKWEKRNI
                     AQEIPEWDPDTCIQCGNCAFICPHAVIRSKIYSPELLKDAPAGFKSADSKIKTFQGQK
                     YTQQISPEDCTGCGLCVEICPAKNKKDPGKKAINLVPVEQIQEEEKANWEFFESIPLL
                     DRTDLQLNKIRDLQFTEPLFEFSGACAGCGETPYLKLISQLFGDRLIIANATGCSSIY
                     GGNLPATPWTINQDGQGPAWSNSLFEDNAEFGYGMRLALDQQKKMAVNLLEELKGEIG
                     QELAEELINAKQTNDEEIQKQRKRVAQLLARLKDISKVEARQLESLAESLIEKSVWII
                     GGDGWAYDIGYGGLDHVIAQKQNVNILVLDTEVYSNTGGQM"
     gene            complement(3094..>4266)
                     /locus_tag="PKJ95_02105"
     CDS             complement(3094..>4266)
                     /locus_tag="PKJ95_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNR65067.1"
                     /translation="ITNIFVPVIITLATLSALFWFFNLSNYQPFLLKAKEIFPWILIT
                     GNKYSFAVFVFISTVVIACPCALGLATPMALVVGSGIAAKKGLIIRNAEAIQISKDVG
                     YVLMDKTGTITEGKPAVIQHTLSEEEMNVVASIESNSHHPLAQAIAGRVKKKIKVGEV
                     KEIAGEGVEANYNGAKYFIGKPEKSGEYTIYQKKGQTVVEVWKDNTKIGFIVLADRIR
                     EDSKSAIISLKKLNITPVMVTGDNEITARAVAGEMGIEEVYAGVKPDEKLSIVRKFQA
                     TGKKVLMIGDGINDAASLKGADIGVAMGKGADLALDSADIVIVKEGISGLIHSITISI
                     NIFKIIKQNLFWAFLYNVLAIPMAMLGLLHPAISEGAMIFSSITVTLNSLRLRKAD"
BASE COUNT         1148 a         1016 c          764 g         1338 t
ORIGIN      
        1 catctgacca ccggtattgg agtagacttc tgtatccaat accagtatgt tgacattctg
       61 tttctgggca ataacgtggt ccaatcctcc gtaacctata tcataagccc agccatctcc
      121 accgattatc cagacactct tttctatcaa tgactctgct aaactttcca attgtcttgc
      181 ctctacctta gaaatatcct ttaatctggc aagtaattgc gctacccgtt ttctctgttt
      241 ttgaatttct tcatcattgg tctgtttagc gttgatcaat tcttctgcca attcctgacc
      301 aatctcgcct tttaactctt ctaatagatt aactgccatc tttttctgct ggtctaaggc
      361 cagcctcatc ccgtaaccaa attcagcatt gtcttcaaag agagaattcg accaggccgg
      421 tccttgccca tcttgattga ttgtccaggg tgtagctggc aaatttcctc cataaattga
      481 ggagcaaccg gtagcattgg caataatcag cctgtcacca aataactgac tgatgagttt
      541 taaatagggt gtttcaccac aaccggcaca ggctcctgaa aattcaaaca atggttcggt
      601 aaattgcaaa tctctgatct tattcagttg cagatcagtt ctgtcaagca aaggaatact
      661 ttcaaaaaat tcccagtttg ccttttcctc ttcctgaatc tgttcaacgg gaaccagatt
      721 gatggctttt ttcccggggt cttttttatt tttagcagga catatctcta cacataatcc
      781 acaaccggta cagtcctccg gtgagatctg ctgcgtatac ttttgcccct ggaatgtctt
      841 tatcttgctg tctgccgact taaatccagc aggcgcatct tttagcaatt caggagaata
      901 tattttactt cttattaccg catgagggca gataaaggca caatttccac attgtataca
      961 ggtatcggga tcccattcag gaatttcctg ggctatattt ctcttttccc atttggtagt
     1021 tccggaagga aaagtcccat cgggctgaaa ggcgcttacc ggtatatcat ctccctcctg
     1081 agctatcatt ctggcagtaa cttcacgcac aaattgagga gctttttctg aaacggtatc
     1141 ttttttcctg atggtgccct ccgctctggc aggcacttta atctccttaa tagacttcat
     1201 agccagatca accgcttcat tgtttctcct gagaatctct tctccacggc taccataaga
     1261 tttttcgata aactgcttga tcttctctat cgctacatct tccggtaaaa ccttgctcaa
     1321 caggaaaaag gcagtctgca ttacggtatt aatcctgaca ccaagaccgg tttctctggc
     1381 aatctgataa gcattaatgg taaatagttt aattttcttg tctattatct tctgctgaac
     1441 ttcccggggt attttatccc aaacctcttc tgccggatag gggctattta agagcagtac
     1501 agcattatct tcagcatatt tcaaaacatc atacttatcc agaaacgaga attgatggca
     1561 tcccacaaaa tttgctctct gaatcaggta tggtgaatgg atggggtctt tcccgaaccg
     1621 taaatgagaa atggttaaag accctgcctt tctggagtca tagacaaagt acccttgagc
     1681 aaaattatct gtttcttccc caataatttt gatagagctc ttgttggcac tgaccgttcc
     1741 atctgaacct aatccataaa ataccgccct tacccgatca actggctcaa tatgaaaatg
     1801 ttcaggatag gaaacgcttc tatgggtgac atcatcgtta ataccaacag taaagtggtt
     1861 taaagggttt tctttcttta tttcctcata tacccccata accattgcag gtgtaaattc
     1921 ttttgatgag agtccatatc tgcctccgaa tacccttatc tttcggtcaa tattgttttc
     1981 actcatatat tcgttaatac cggataagac ttcagtatac aaaggttctc cagcactgcc
     2041 cggctctttg gttcggtcca ggacacacag tatcttcgcg gtctccggta tagcttgata
     2101 gagatgcctg actgagaaag gtctgaacag acgaaccttt atcataccta ccttttcacc
     2161 ctttccaatc aaatgctcta cggtctcctg taccgtttcg cagcctgagg ccataatcag
     2221 aaatacatat tcagcatcag tagcgccttc atactggaag agatggtatt ccctgccggt
     2281 aatctcttta aattctttca ttgtctcttc ggtaatatcg gcacatatat cataaaaggg
     2341 attacaccct tctcttgcct ggaaaaaaac atcaggattt tgggctgtac ctcgtataaa
     2401 agggcgttcc ggggataagg cgcgagccct gtgctcatag accagtttat catcaatcat
     2461 tctcttcatc tcttcaactt ccagaagttc tactttggat atctcatggg aagtacggaa
     2521 tccatcaaag aaatgcaaaa agggaattct ggatttcaga gaagctgcct gggcaatcaa
     2581 gggaaagtcc atcgcctctt gtacggaagc cgctgctaac aaggcaaatc ccgtggttct
     2641 ggcagccatt acatcagaat ggtctccgaa tattgacaga gcatgtgttg ctaccgtgcg
     2701 ggcagacacg ttaaatacca ccggtgacag ttctcctgaa attttaaaca tattgggaag
     2761 cataagtaac aaaccctgtg aagccgtgaa ggtagtagtc aaagcccctc tttgtaaaga
     2821 gccatgaacc gcacctgcgg ctccagcctc actctgcatt tcagctacag caggcactgc
     2881 accccagata tttttctttc cctgaaatgc ccacaaatct gccagttccc ccataacaga
     2941 agatggtgtt atgggataaa tggtaacatt ttcactgata tgatatgcag tataagcagc
     3001 tgctgtattg ccgtcaaagg caactaattt atgtttcatt tcaaatcact ccttttatta
     3061 atattttttt gtatacctca atggtaagta gttttaatcg gcctttctaa gccgcaatga
     3121 attcaaggtg actgtaatag agctaaaaat catagctccc tctgatatag caggatgtaa
     3181 taatcccagc atggccatag gaatagccag aacattgtac agaaaagccc agaaaagatt
     3241 ctgttttata attttaaaaa tattaataga tatggtaata ctatgtataa gcccggaaat
     3301 tccttccttc acaattacta tatcggcact atctaaagct aaatctgctc ccttgcccat
     3361 tgccaccccg atatctgctc cttttagaga ggcggcatcg ttaattccat ctccaatcat
     3421 caaaactttc tttccggtag cctggaattt cctgactatg gacagttttt catctggctt
     3481 tactccggca tatacctctt caatacccat ctcacccgct actgcccgtg ctgtaatttc
     3541 attgtcaccg gtaaccatta ccggtgtaat gtttagtttc tttagactaa ttatagcact
     3601 ttttgaatct tctcttattc tatcagccaa aacaataaaa cctatctttg tgttatcttt
     3661 ccaaacctct accactgttt gacctttttt ttgataaatt gtatattctc cacttttttc
     3721 tggtttaccg ataaaatatt ttgccccgtt gtaatttgcc tctacaccct ctccggcaat
     3781 ctcctttact tcccccactt ttatcttctt ttttactctg ccggcaatgg cttgtgccag
     3841 gggatggtgt gagttgcttt caatggatgc aactacattc atctcttctt ctgacagagt
     3901 atgctggata acagccggtt taccttcagt tatagttcct gttttatcca tcaatacata
     3961 tcctacgtcc ttggaaatct gtatcgcctc agcatttcgg ataattaatc ctttttttgc
     4021 cgcaattccg ctccctacca ccagagccat tggagtggcc aatcctaaag cacagggaca
     4081 ggcaattacc acagtggaaa taaagacaaa gactgcaaaa gagtatttat ttccggtaat
     4141 taaaatccag ggaaatattt cttttgcctt taataagaat ggctgataat tgctcagatt
     4201 gaaaaaccag aacaaggcac ttaaggtggc aagggtaatg ataacgggaa caaagatatt
     4261 agtgat
//
LOCUS       DAOQWT010000066         4256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_21191_length_4256_cov_3.105451, whole genome shotgun sequence.
ACCESSION   DAOQWT010000066 DAOQWT010000000
VERSION     DAOQWT010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21191_length_4256_cov_3.105451"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PKJ95_02110"
     CDS             <1..273
                     /locus_tag="PKJ95_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65068.1"
                     /translation="GVSAFFFVIFLFDKRHGKEKEAQFGLFFGAMCGGLEQNLKKRLN
                     DARCEMEYKKYYDYVVVNDELEKAARKLEAIIVSERDKCKKSQNSL"
     gene            452..1663
                     /gene="coaBC"
                     /locus_tag="PKJ95_02115"
     CDS             452..1663
                     /gene="coaBC"
                     /locus_tag="PKJ95_02115"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNR65069.1"
                     /translation="MNILLGITGGIAAYKSAELIRLMKKEGFQVKVVMTANATRFITP
                     LTMEVLSENKVYIDLFSEDERGYRISHISLARWADMVVIAPATANIVSKLAHGVADDL
                     LSTTLLAFQGSVILAPAMNKVMYNNPIFRDNIHYLMKKGYHFIETGKGSLACGEYGEG
                     RMAEPPQIVGFVKELISKSNDLKGKKILITAGATREPIDQVRFISNYSSGKMGFALAE
                     VAQKRGAEVILISGPTFLEDIEGINTIRVDTAADMREQVFTYFKEADVFISAAAVSDF
                     RPARQFEGKIKKEEQNIIHLELKKNVDILKEAGEKKDKQILIGFAAEVKSLKENALSK
                     LESKNLDYIIANDITREDSGFGTDTNKVIIIERNGNTIDLPLMSKYEVAKYIFDLIKS
                     HFERQSKGRIV"
     gene            1663..3645
                     /gene="priA"
                     /locus_tag="PKJ95_02120"
     CDS             1663..3645
                     /gene="priA"
                     /locus_tag="PKJ95_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNR65070.1"
                     /translation="MSKNNVAEVIPLKIYLEHSFYYLIPSSLQEAAEVGKRVIIPFRK
                     SKKVGIIVNIIAETEFPDLKEIEEVLDPVPILSREVLFLTDWIAGYYLCPRGVVISSI
                     LPSRISSKKITAFFNDNTIERIIFDRNRIPIDKEDKPILFQCLSYRERDLYYTHLIVE
                     TVKEGKQVLLLVPDQFSCKDLKEKLAKKLGHSLAIFDKNVSQTEKYLRFIMVQSEDIK
                     VVIGTRSSIFLPFLNLGLIIVEQENSPLYKEERTPRYHAREVAQRRGLLESAQVILAS
                     ASPSIESYWYGLNNDFLLKTKYRVHRATEKNWLQTYLVDLEEEKSFQRVISFQLQQQI
                     VECLKEKKGVVLFLKRRGFASYIVCTHCGLVIKCPECNSLLSYNEIERKGVQVCNKCG
                     KRIPYNRYCPKCGEKALKPMGFGTQYVEEITRRMFPKAIIQRFDKDIAPNLRIQQKLL
                     NKFKEGEIDILIATQLLLNRLNYQKVSLVAFILVDHLFNIPDYRSAESTFQIIYQITL
                     HLMEQKTPKVLLIQTCHPEHHSLQAATELNYLSFYWKEISFRKELEYPPFTKIIRIDF
                     TGTSEEPVKKSAWQFRQFINKSNILTTLGKDIFLNDAYPLVVKDKDKSRISFLIRIKN
                     EKEICEQLKEMLLPYFLKYQKHQVKIIADVEPIRLY"
     gene            3663..4148
                     /gene="def"
                     /locus_tag="PKJ95_02125"
     CDS             3663..4148
                     /gene="def"
                     /locus_tag="PKJ95_02125"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNR65071.1"
                     /translation="MAVLKLHKYGSPILREKALPVKKVDEEVKELVENMIETMYVEGG
                     VGLAAPQVGVSKRIIVIDTEEEGVIVLINPVIIKREGESKEEEGCLSVPGIYSPVRRS
                     SIVTVEAMDLNEKKIQITQEGFLAIALQHEIDHLDGYLFIDRLSPAKRLMIKDELKNI
                     E"
BASE COUNT         1489 a          650 c          902 g         1215 t
ORIGIN      
        1 ggtgtgtcgg cctttttttt cgtcattttc ttatttgaca aaaggcatgg gaaggaaaag
       61 gaggcacagt tcgggctgtt tttcggcgcg atgtgcgggg gtcttgagca aaatcttaaa
      121 aaaaggttaa atgatgctcg ctgtgaaatg gagtacaaaa aatattatga ttatgtagtg
      181 gtaaatgacg agctggagaa agctgcccga aaattggaag ctataattgt cagcgaaaga
      241 gacaaatgta aaaaaagtca aaatagtctt tagacgttgg tatcctttat ttagttgaaa
      301 tcattgcggg ctgctatttt tatctaaaaa ctatgagtta caaaccaaaa acttcataac
      361 ttacgaccaa tgacagattt aatggtatac ttaatatgtt agaataatta gggaccatag
      421 ttacaatata tctgaagaaa aggataaaaa aatgaatatt ttacttggta ttaccggtgg
      481 gattgcagcc tataaaagtg cagaattaat ccgcttgatg aaaaaagaag gatttcaggt
      541 aaaagtggtg atgaccgcaa atgccacccg ttttatcaca cctttaacaa tggaagttct
      601 ttctgaaaat aaagtttata tagacctttt ttctgaagat gaaagaggtt ataggataag
      661 ccatatttct ttagctcgct gggcggatat ggtggtaatt gctccggcaa ccgcaaatat
      721 tgtttccaaa ttagctcacg gagttgcaga tgacctgctc agcaccactt tgctggcttt
      781 ccagggcagt gttatccttg ctcctgccat gaacaaggtc atgtataata atccaatatt
      841 cagagacaat attcactatc taatgaaaaa gggttatcat tttatagaaa caggaaaggg
      901 cagtttagcc tgcggagaat atggtgaagg aaggatggct gaaccacctc agatagtcgg
      961 gtttgtaaaa gaattaataa gcaaaagcaa tgatttaaag ggaaagaaga tacttataac
     1021 tgccggagca accagagaac ctattgacca ggtaagattt atttccaatt actccagtgg
     1081 aaagatgggt tttgcactgg ctgaagttgc tcaaaaacgg ggtgctgagg taattctcat
     1141 ctctggacct acctttctgg aggatattga gggaataaac actattcggg tagatacagc
     1201 agctgatatg agagagcagg tgtttaccta ttttaaagag gcagatgtat ttatttctgc
     1261 ggcagctgta tcagattttc ggcctgcgag gcaatttgag gggaaaatca agaaagaaga
     1321 acagaatata attcatttag aactaaagaa aaatgtggac atattgaaag aggcaggaga
     1381 gaagaaggat aagcagatac tgataggatt tgcggcggaa gtgaaaagtt taaaagaaaa
     1441 tgccctctca aaattggagt caaagaattt agattatatt atagccaatg atattacccg
     1501 tgaagacagc ggatttggaa ctgatactaa taaggtaatc attattgagc gcaacggaaa
     1561 tactattgat ttacccttga tgtcaaaata tgaggttgca aagtacattt ttgatttgat
     1621 aaaatcacat tttgagcgtc agagtaaggg tagaatagta taatgagtaa aaacaatgtg
     1681 gcagaggtta tccctctcaa aatatatctt gaacatagtt tttattacct cattccttcc
     1741 tctttacaag aggcagccga agtaggaaaa agggtgataa ttccctttcg aaaaagtaaa
     1801 aaagttggaa ttatcgtaaa tattattgca gaaactgaat ttcctgattt aaaagagatt
     1861 gaagaggtgc tggaccctgt tcccattcta tccagagaag tcctcttttt aactgattgg
     1921 atagccgggt attatctgtg ccccagaggg gttgtaatct catccattct accctcacgc
     1981 atttcctcaa agaaaattac agcttttttt aatgataaca ccatagaaag aattattttt
     2041 gacagaaata gaattccaat tgataaagag gataaaccga tactctttca atgccttagt
     2101 tatagagaaa gggacctcta ttatacccat ttgattgtgg agactgttaa agaaggcaaa
     2161 caagtattac tccttgtgcc agaccagttc agttgcaaag atcttaaaga aaaactggcc
     2221 aaaaaattag gtcattcttt agccattttt gataaaaatg taagtcagac tgaaaagtat
     2281 ctgcgcttta ttatggtaca aagtgaagac attaaagtgg taataggtac cagatccagt
     2341 atttttctac cctttttaaa tcttggttta ataatagtgg aacaggaaaa ctcccctttg
     2401 tataaagagg aaagaacccc ccgttaccat gcccgggaag ttgctcaaag aagagggtta
     2461 ttagagtccg cacaggtaat tctggcctca gcatcaccat caatagagag ctattggtat
     2521 gggttaaata atgactttct tttaaaaact aagtataggg tacatagagc tacggaaaag
     2581 aattggttac aaacatatct ggtagactta gaagaagaga agtcttttca aagggtaatt
     2641 agttttcagt tacaacagca gatagtggag tgccttaagg aaaaaaaagg agtggttcta
     2701 tttcttaaaa ggcgtggttt tgccagctac attgtctgca cacactgcgg tctggtgata
     2761 aaatgtccgg aatgtaattc gctcctctct tataatgaga tagagagaaa gggtgttcaa
     2821 gtttgcaaca aatgtggaaa aagaatacct tataacagat attgtcctaa atgtggagaa
     2881 aaagctctga agccaatggg ttttggaacc caatatgtgg aagaaataac ccgtcggatg
     2941 tttcctaaag ctattattca acgctttgat aaagatattg ctccaaattt gaggattcag
     3001 caaaagttgc tcaataagtt taaagaggga gaaatagata tactaattgc tactcagtta
     3061 ctgctgaaca gattaaacta tcagaaggta agcctggtgg catttatatt agttgaccat
     3121 cttttcaata ttcccgatta ccgctcagca gagagcactt ttcaaatcat ttatcagata
     3181 actttacatt taatggaaca aaaaactccc aaagttctat taattcaaac ctgccatcct
     3241 gaacatcaca gtttgcaggc ggcaacagag ttaaactacc tctcatttta ttggaaagag
     3301 ataagtttta gaaaagagtt agaatacccg cccttcacca agataattag aattgatttt
     3361 acggggacca gcgaagagcc ggtcaaaaaa agtgcctggc agtttagaca atttattaac
     3421 aaatctaata tattaaccac tttggggaag gatatctttt taaacgatgc ctacccgctt
     3481 gtggttaaag ataaagataa aagcagaatc agttttctga ttagaattaa aaacgaaaaa
     3541 gaaatctgtg aacagttaaa agaaatgttg ctaccctatt ttttaaaata tcaaaaacat
     3601 caggtgaaaa taatagcaga tgttgaaccc ataagattat attaatatgg caggaggaga
     3661 aaatggctgt tttaaaatta cataaatacg gctctcctat tttaagggaa aaagccttgc
     3721 cagttaaaaa agtagatgaa gaagtcaagg agctggtgga aaatatgatt gaaacaatgt
     3781 atgttgaagg aggtgttggt ctggcagctc ctcaggtagg tgtttctaaa cggataattg
     3841 taatagatac agaagaagag ggagtaatcg tattgattaa cccggttatt atcaaacggg
     3901 aaggtgaatc aaaagaagaa gagggatgcc tgagtgtacc agggatatac tctccggtac
     3961 gaaggtcctc aatagttact gtagaggcta tggatttaaa tgaaaaaaag attcagataa
     4021 cccaagaagg ttttctggca attgctttac aacatgaaat tgaccatctt gatggctatc
     4081 tgtttataga tagattgagt ccggctaaga gattaatgat aaaagatgaa ttaaaaaata
     4141 ttgaatagct atatacaaaa aggggagaag atactattgt gaaaattata tttatcggta
     4201 catcatcctt tggtgtttct atattggaga aattaatcag tcttaagaag gatata
//
LOCUS       DAOQWT010000067         4189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_21650_length_4189_cov_3.571843, whole genome shotgun sequence.
ACCESSION   DAOQWT010000067 DAOQWT010000000
VERSION     DAOQWT010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21650_length_4189_cov_3.571843"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKJ95_02130"
     CDS             <1..336
                     /locus_tag="PKJ95_02130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463466.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNR65072.1"
                     /translation="TATIVGKRHYQIAHQIKQALSEYEELKDIIAMLGLEELSKEDQN
                     IVNRARRLERFLTQPFVTTEQFTGNAGRRVSLREALDGCEQILNDEFYDYPESALYMI
                     GSIDEAKRL"
     gene            333..722
                     /locus_tag="PKJ95_02135"
     CDS             333..722
                     /locus_tag="PKJ95_02135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNR65073.1"
                     /translation="MNLTILLPEKTYWQGKVKKVVGEAKNGSFCLLPAHIDFVTIMVP
                     GIFYTVTEEDQEIYIAINEGVLLKTGREVTLATRNAAKGDSLGSLKKQVEEDFKRINQ
                     QDRRANQALQKLEADFVRRFLDLEAYG"
     gene            715..1047
                     /locus_tag="PKJ95_02140"
     CDS             715..1047
                     /locus_tag="PKJ95_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNR65074.1"
                     /translation="MDKKSEHSDIKKEEEFMNEIESKEKRKIRAQSKKNRAIWMGFGL
                     FGVVGWSVMIPTIIGIIIGLWLDRKLTGRISWTLTFLILGIALGCWNAWYWVQKERKL
                     IEKEREEE"
     gene            1084..1383
                     /locus_tag="PKJ95_02145"
     CDS             1084..1383
                     /locus_tag="PKJ95_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HNR65075.1"
                     /translation="MVLGCLSGLFYFGGLWLTLCHMSKARYPIVLTILSYVIRTAIIF
                     VILYFIARSGKWASILFWLMGFIVTRIVLASLLKGNYLDKKKFSSDKVRQKEGEE"
     gene            1387..2100
                     /locus_tag="PKJ95_02150"
     CDS             1387..2100
                     /locus_tag="PKJ95_02150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNR65076.1"
                     /translation="MKITPDHIIYWQWEGFNLNATIVFTWLVMLVLVLISWLATRNLV
                     VKPKMSRWQVSLEVVIGFIREQIREITQQNPDPFIPFLGSLFLFISLSNLLDIIPGFR
                     PPTGSLSTTAALAICVFFAIPIYGISRIGVKKYLKQYLEPSVLMLPFNIIGEFSRTLA
                     LAVRLFGNIMSGSLIAGILLIIAPLFLPVIMQVFGLLIGQIQAYIFTILATVYIASAA
                     QAENELQMKKGKEGSENNE"
     gene            2093..2362
                     /locus_tag="PKJ95_02155"
     CDS             2093..2362
                     /locus_tag="PKJ95_02155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNR65077.1"
                     /translation="MSSVDLIAVASIFTAGITMAIGSIAPAIGEGLALARALGAIAQQ
                     PDEANTITRTLFVGMAMVESTGIYCLVISIILIFANPFWNYILSQ"
     gene            2372..3187
                     /locus_tag="PKJ95_02160"
     CDS             2372..3187
                     /locus_tag="PKJ95_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65078.1"
                     /translation="MIVDLFTVIVQIVNFLILLFLLKKFLFNRIINIIDERENQIGKQ
                     FAEAEQKEKDAKLELEKQRKGREKLEREWEDNLIKIKREIEAERGKMMEEARVAVNEA
                     QGEWERAIRAQRDAFLKELRKLSCQQVFLISKKVLADLADNRLEDQLINSFIAQLEGL
                     DGRQRQEFKRDEFKGSGGQIRKNKVEINTAFPLSEEAKDLLIRKVKQLVDRDALPVFY
                     VYPDLICGIELKTESKKIAWSMEHYLDLLEMGFEDIFMGNKEIVNLKDKQDIT"
     gene            3308..>4189
                     /locus_tag="PKJ95_02165"
     CDS             3308..>4189
                     /locus_tag="PKJ95_02165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196576.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNR65079.1"
                     /translation="MSLDEAVKNFKAVLDIQEIGIITSVGKGVVRANGLPNAKSGEIV
                     TFKGGNLGMVFNLNPDSVDIILLDKSKTLSSGDEVRRTNRVMDVPVGEALLGRIIDPA
                     GRPLDERGRINYVERLPVEQEAPPIMHRDPVNVPLQTGLLVVDALVPVGRGQRELILG
                     DRQTGKTAIAVDTVLNQQKGDVICIYCSIGQKLSSVTRVIAKLKESGAIDYSIGVIAS
                     GEDTPGLNYIAPYAATSIGEYFMRKGKDVLIIYDDLTHHARSYRELSLLMRRPPAREA
                     YPGDIFYIHSRLLERATH"
BASE COUNT         1320 a          670 c         1001 g         1198 t
ORIGIN      
        1 actgctacta ttgtgggcaa aagacattat cagattgcac accagataaa acaggccctc
       61 tccgagtacg aggaactgaa agatattatt gctatgttag gattggaaga attgtccaaa
      121 gaagaccaga atatagtaaa ccgagccaga agattggaaa gattcctcac ccagcccttt
      181 gtaactacgg aacaatttac cgggaatgcc gggcggagag tatcattgag agaagcatta
      241 gatggatgtg agcaaatttt aaatgatgag ttttatgatt atccggagag cgctttatat
      301 atgatagggt ccatcgatga ggcgaagagg ttatgaatct gactattctg ctgccagaaa
      361 agacttactg gcagggcaaa gtgaaaaaag ttgtgggaga agcaaaaaat ggctcttttt
      421 gccttcttcc ggcacatatt gattttgtaa ctattatggt ccctggtatc ttttatactg
      481 ttacagaaga ggatcaggaa atctatatag ccatcaatga gggggttcta ttaaaaaccg
      541 gtagagaggt taccttagct accagaaatg ctgccaaagg ggacagtctg ggtagtctga
      601 aaaagcaagt agaagaggat tttaaaagga ttaatcagca ggacagaagg gctaaccagg
      661 ccctgcaaaa attagaagct gattttgtga gaagattttt ggatttagaa gcctatggat
      721 aaaaaaagcg aacattcaga tattaaaaaa gaagaagaat ttatgaatga gattgaaagt
      781 aaggaaaaaa gaaagataag agctcagagt aaaaaaaatc gggctatctg gatgggattt
      841 ggcttattcg gtgttgtggg atggtctgta atgattccca ccattatagg aattatcata
      901 ggattatggt tggacaggaa attgaccgga cggatatcct ggactttgac ctttctcatt
      961 ttaggcattg ccttgggttg ctggaatgcc tggtattggg tacaaaagga acgcaagctg
     1021 attgaaaaag agagagaaga agaatagaga gagggagtaa aaataattaa tcaattcttt
     1081 tttatagttt taggttgttt atcaggttta ttctattttg gtgggctctg gcttaccttg
     1141 tgccatatga gtaaggcaag atatcctatt gtgctaacca tactcagcta tgttattaga
     1201 actgccatta tttttgtgat attatatttt attgcccgct caggaaagtg ggcttctatt
     1261 ctattctggc taatgggatt tattgtaacc agaatagtat tagccagtct cttaaaagga
     1321 aactatctgg acaagaagaa gttttcttct gataaagtac ggcaaaaaga aggggaggaa
     1381 taagaaatga aaattacccc tgatcacata atatactggc agtgggaggg atttaaccta
     1441 aatgccacta tcgtttttac ctggctggtg atgctggttc tggttttgat tagctggttg
     1501 gctaccagaa atctggttgt aaaacctaaa atgagccgct ggcaagtctc tttagaggtg
     1561 gtaattggtt ttatccgtga acagattaga gagattaccc agcagaatcc tgacccgttt
     1621 attccttttt tgggaagcct ctttttattt atctctcttt ccaatctgct ggatataatt
     1681 ccagggtttc gtccgccaac cggttcactt tcaacaacag cggcactggc tatttgtgtc
     1741 ttttttgcca ttcccatcta tggaatttca aggattggcg tgaaaaaata tctcaaacag
     1801 tatctggaac catcagttct gatgctgccc tttaatatca tcggggagtt ttcacggact
     1861 ttagccctgg cagtacggct ctttggaaac attatgagcg gctccttaat tgccggaatt
     1921 ctattgatta ttgctcctct atttcttcct gttattatgc aggtattcgg gctgttaata
     1981 ggccagatac aggcttatat atttaccatt cttgctacgg tttatatagc atctgcagca
     2041 caggcggaga atgaactaca aatgaaaaaa ggaaaggaag gaagtgaaaa taatgagtag
     2101 tgttgattta attgcagtcg cctcaatttt tacagccggg attactatgg ctatcggctc
     2161 catagctcct gctatcggag agggtttagc tttagccagg gcacttggtg ccattgccca
     2221 gcagcctgat gaggccaata ccatcaccag aacgttattt gtgggcatgg ctatggtcga
     2281 atccacggga atttattgtc tggtaatttc tataattctt atctttgcca atcccttctg
     2341 gaattatatt ttaagtcaat aggggaagat tatgatagtt gatctattta cagtcatagt
     2401 tcaaattgtt aactttctga tcctgttatt cctgctcaaa aaatttcttt ttaacaggat
     2461 aataaatatc atagatgaga gggagaatca gattggcaag caatttgctg aagctgagca
     2521 gaaagaaaaa gatgcaaaac tggaacttga gaaacagaga aaaggcagag agaaactgga
     2581 aagggaatgg gaagataatc tgataaaaat aaaaagagag attgaagcag agcggggaaa
     2641 aatgatggag gaggctagag tcgcagtcaa tgaggcacag ggggaatggg aaagagctat
     2701 acgtgcccag cgtgacgctt ttttgaagga gttgaggaag ctttcctgcc agcaggtatt
     2761 cctgatctct aaaaaagtat tagctgatct ggctgataac agattagaag atcagttaat
     2821 caacagtttt attgctcaat tggaaggatt ggatgggcgt caaaggcagg aatttaaaag
     2881 agatgagttt aaagggtcag gcgggcaaat caggaagaat aaagtagaaa taaataccgc
     2941 ctttccctta agtgaagaag caaaggattt actgataagg aaggttaaac aattggtaga
     3001 tagggatgca cttcctgttt tttatgttta tccggacctt atttgtggaa ttgaattaaa
     3061 aacggagagt aaaaagattg cctggagtat ggagcattat ttagatctgt tggagatggg
     3121 attcgaagat atttttatgg gaaacaaaga gattgtcaat ttaaaagata aacaggatat
     3181 tacctagttt ccctttgttg ggagatgttg gaatttaata aaaaaacatt tgtgactggt
     3241 tttagaaaaa aaagtgaaaa aagacaataa tccaatttta ttgggaaaag aaataaaaaa
     3301 aacatttatc tctctggatg aggcagtaaa aaattttaaa gctgtactgg atattcagga
     3361 gatcggtata attacctcag taggtaaagg agttgttaga gcaaatggtc tacccaatgc
     3421 caaatcagga gagatagtga ccttcaaggg tggaaactta ggtatggtct ttaacctgaa
     3481 tcccgatagt gttgatatta tacttttaga taaatccaaa accctgtctt ccggggatga
     3541 ggtaagaaga actaatcggg taatggatgt accggtggga gaggcgctgc tgggtcgtat
     3601 tatcgaccct gccgggagac ctcttgacga gagaggtcgg ataaattatg tggaaagatt
     3661 gccggttgaa caggaagcac cacctattat gcatcgtgac ccggtaaatg tcccactaca
     3721 aacaggatta ttggttgttg atgctctggt acctgtgggc agaggtcagc gtgaactgat
     3781 tctgggtgac aggcaaaccg gtaaaactgc tattgctgtt gatacagtat tgaatcagca
     3841 gaaaggcgat gttatatgta tctattgctc tatcggtcaa aagctatctt cagttaccag
     3901 ggttattgct aaattaaaag aatcaggagc aatagactat agcattgggg taatagcatc
     3961 gggagaagat actcccggct tgaattatat tgcaccatat gctgctacca gcattgggga
     4021 atattttatg agaaagggca aagatgtact tatcatctat gatgatttga cccatcatgc
     4081 ccgttcatac cgggaattat ctttattgat gagaagaccg ccggcaagag aagcctatcc
     4141 cggtgatata ttttatatcc attcccgcct gttagagagg gctacccac
//
LOCUS       DAOQWT010000068         4169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_21790_length_4169_cov_6.934127, whole genome shotgun sequence.
ACCESSION   DAOQWT010000068 DAOQWT010000000
VERSION     DAOQWT010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21790_length_4169_cov_6.934127"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..919)
                     /locus_tag="PKJ95_02170"
     CDS             complement(26..919)
                     /locus_tag="PKJ95_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNR65080.1"
                     /translation="MDNTYGPNCRISYYYYPTREAATRARRGIVKYQKFKEECLHKET
                     KIIDCRVTKQGQMLKEERCKVCDKHIRWISGEKKGKHEFLNILPVSPVFFGQITNEFL
                     KYTSEYNSPQEITREVVMGRKEHNKFNTYTKEIIVCETKSYWKDVIEKIVENKELALE
                     FARYCLKKREIDFIEKPQITIFNENIESVIEQRLYENIKNKLPKNCCIIPQYNIPQTY
                     YKVDFFITDIHENIKLVIECDSYEWHHKTPDQILFDYKRMRKIISLGYTYFPFTGREL
                     HSEPTECVKEILNLLERINNL"
     gene            complement(1552..1848)
                     /locus_tag="PKJ95_02175"
     CDS             complement(1552..1848)
                     /locus_tag="PKJ95_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65081.1"
                     /translation="MKWRKQNKQKCLDAARRYRERHREEIRLREKQGDRKKYYKKWRK
                     QNKEKMREYHRNYYREKRSVKSEAVKKEDKKVKRLIHKIIVKMEEERKYAGTSL"
     gene            complement(1811..2227)
                     /locus_tag="PKJ95_02180"
     CDS             complement(1811..2227)
                     /locus_tag="PKJ95_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65082.1"
                     /translation="MYLVKQPLPKEIKFMIREGILPEKTEVYLLDYNTVIFSKESWGK
                     GQERLHASISHPFRLPAWEEVKFVRYTLFPDEMTVAQILPPKSEYVDMHKNCFHLWEL
                     KKEEICRDSQREEGTGAQEKTGNNHEVEETEQTKVP"
     gene            complement(2229..2639)
                     /locus_tag="PKJ95_02185"
     CDS             complement(2229..2639)
                     /locus_tag="PKJ95_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65083.1"
                     /translation="MSYKILRGRGLISMTDPNGKKIPFGRMEEIAISGKEFVDEVGEA
                     IELLNIMAKKRNGILTIKEGNKGGMYLIYFMSKEKLQIDYLVYIDLLERKMYPTVIMK
                     RWLLEEEIEKEKLCEIIPEAITEDVKALEIIKEG"
     gene            complement(2736..3062)
                     /locus_tag="PKJ95_02190"
     CDS             complement(2736..3062)
                     /locus_tag="PKJ95_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65084.1"
                     /translation="MTGTIEIRVCTACGKPVESRDFYRLTKDKTRLTSECKKCKKLRR
                     KLYYEKNKEQVNEKHPNTYTKKHVIGDNPIDVFNKTLEEQGICWLDELRKYHQERWGK
                     KDEGEN"
     gene            complement(3173..3466)
                     /locus_tag="PKJ95_02195"
     CDS             complement(3173..3466)
                     /locus_tag="PKJ95_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNR65085.1"
                     /translation="MLSEVKLNHIYQGNCKRSQAKWREAGFETPPVTFQGLKQCDCNA
                     GFKPGIVLDPFMGSGTTAKIAMEQNKNFIGIELNPEYIKLAEKRIAHVQVRLF"
     gene            complement(3445..3993)
                     /locus_tag="PKJ95_02200"
     CDS             complement(3445..3993)
                     /locus_tag="PKJ95_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65086.1"
                     /translation="MYNFKHLYGPEHFIPAPNSSNKFWSVLFGYMEKYSKTGDSLLLV
                     AEPKEVLPVFKEKFKLSKIDTLGYSGEKGKDYEVDLNIQDSLLIARGLRKYNFVFSQA
                     LLEHICRPSIAIENMVGALNSGGYLILHTHNVEMPYHGFPIDCCRFYKDFFVDLCKYL
                     PIEIIEYDEWGRNIFVCYRKLN"
BASE COUNT         1060 a          899 c          534 g         1676 t
ORIGIN      
        1 tcttccggga tagaattaaa gttaatcata agttattaat cctttctaat aaatttagta
       61 tttcttttac acattcagtt ggttcactat gtaattctct tcctgtaaat gggaaatagg
      121 tatatcctaa agatataatt tttctcattc ttttataatc aaatagaatt tgatctggtg
      181 ttttatgatg ccattcataa ctatcacatt caattacaag tttaatattt tcatgaatat
      241 ctgtaatgaa aaaatctacc ttgtaatatg tctgaggtat attatattgg ggtataatac
      301 agcagttttt aggcagttta ttttttatat tttcatataa tctttgttct attaccgact
      361 ctatattttc attaaatata gttatttgag gtttctctat aaaatcaatt tctcgttttt
      421 ttagacaata tcttgcgaac tctaaagcca gttctttatt ttccactatt ttttctataa
      481 catctttcca ataactttta gtttcacata cgataatttc tttcgtgtat gtattaaatt
      541 tattatgttc tttcctcccc ataactactt ctcttgttat ttcctgcggt gaattatatt
      601 ctgaagtgta ttttaggaat tcgttagtta tttgtccgaa gaaaaccggg cttactggta
      661 aaatattaag aaattcatgc ttacccttct tctccccact tatccacctt atatgtttat
      721 cgcacacttt acatctttct tctttgagca tttgcccttg ttttgttact ctgcaatcta
      781 ttatttttgt ttctttatgc agacattctt ctttaaattt ttgatatttc acaatccccc
      841 tccttgctct ggttgcagcc tcccgtgtag gataatagta ataacttatc ctgcagttgg
      901 gcccataggt attatccata gttcctatat aataccctgc cccggacata agcacctgta
      961 cttcataacc ttttttacac tgcatattta attaaccttc cttccgtttt ttagaattaa
     1021 acctttagga ttttttgttg tttttttagc gcatgtgata ttacatataa aattaagttg
     1081 tctactaaat gaatttgtta ttcttcttgc atacatccaa tggagaacat aaaagggatt
     1141 gtttgcaagt agtaattcag gtctattttc ttttaaatag ttataatttt ctgtgcaaat
     1201 ttcgcataaa tatctttcgt taaaaataat tgtttcttca tcatgctcta tttttctata
     1261 gcaacctcca catattttag tattattttt atttttctct ggtatttcat cacacttaca
     1321 acaaggtttt ccgatattcg ctaggttgaa aaaatgtttc cactttccat taattttttt
     1381 attataatag tatgatcctt ctgctgattt atctttgcaa tgattacatt taaaacaaaa
     1441 atgttttaat attttttctc ctcttcgtta gttaattttt cttggccgta cggtaaataa
     1501 ccataactac agccgcattg tttgcatctt atataccctc tgttatctat ttcataagga
     1561 agttcctgca tattttcttt cctcctccat ctttactata atcttatgta tcagtcgctt
     1621 tactttctta tcttcttttt taaccgcctc tgactttacc gatctttttt cccggtaata
     1681 gttcctatga tattccctca tcttctcctt attctgtttc ctccactttt tataatattt
     1741 cttccggtct ccctgctttt ccctcagtct tatttcttcc ctgtgccttt ccctgtatct
     1801 tctcgctgca tcaaggcact tttgtttgtt ctgtttcctc cacttcatgg ttatttccag
     1861 ttttttcctg tgcgccagtt ccttcctctc tctgactgtc acgacatatt tcctcctttt
     1921 taagctccca caggtgaaag cagtttttat gcatgtcaac atattcactt tttggcggta
     1981 atatctgcgc taccgtcatt tcatccggga acagtgtata tcttacgaat ttaacctcct
     2041 cccaggcagg cagacgaaac ggatggctta tagaggcatg caatctctcc tgaccctttc
     2101 cccaggattc ttttgagaat atcacagtgt tgtaatctaa cagatatact tctgttttct
     2161 ccggcaggat tccctcccgg atcataaatt taatttcttt tggtaacggt tgttttacca
     2221 gatacatctt acccctcctt aataatttcc agagctttaa catcttctgt tattgcctcc
     2281 ggtataattt cacataattt ttctttttct atctcttctt ctaaaagcca tcttttcata
     2341 ataaccgtag gatacatttt tctttcaagt aaatctatat ataccaggta atcaatctga
     2401 agtttttctt tagacataaa atatattaga tacatccctc ctttattacc ttctttgatt
     2461 gttaaaattc cgtttctttt cttcgccatg atatttaata attctatagc ctctccaact
     2521 tcatcaacaa actctttccc ggagattgct atttcttcca tacggccaaa aggtattttc
     2581 ttcccatttg gatctgtcat agatattagt cctcggcctc ttaatatttt gtaactcatg
     2641 ataaatccat cctgttctgc aacgcgagca taaccctatc cggctcaact tcaaataagc
     2701 tgcataatat taaaattaat tcggctatgt ctttttcagt tctctccttc atccttcttt
     2761 ccccaccttt cttgatgata tttcctgagt tcatctaacc aacatatccc ctgctcctcc
     2821 agagttttat taaacacatc tatagggtta tctcctatga catgtttctt tgtataagtg
     2881 ttcgggtgtt tctcattaac ctgctctttg tttttctcgt aataaagctt tcttctgagc
     2941 ttcttacatt tcttgcactc actggttaac ctggttttat ctttagtcag cctgtaaaag
     3001 tccctgcttt ctaccggttt accgcaggca gtgcaaactc ttatttctat ggttcctgtc
     3061 atatccctac ccctttctat tttgcgtttt aacgatgctt ttatcaagat cgtaggattt
     3121 tacgttttcg tatttctctt taatcctacc ccctatttcg accctgtttt aattaaaata
     3181 atctgacctg cacatgtgca attctttttt ctgccagttt gatatattcc gggttgagtt
     3241 ctatacctat gaaattttta ttctgttcca tagctatttt tgcagtcgtg ccgcttccca
     3301 tgaacggatc taacactatg ccgggcttaa accctgcgtt acagtcacat tgctttaatc
     3361 cctggaaggt aacaggcggt gtttcaaatc ctgcttccct ccattttgcc tgacttcttt
     3421 tacagttacc ttgataaata tggtttaatt taacttccga taacatacaa atatattcct
     3481 tccccattcg tcatattcta ttatttcaat cggcaggtat ttacagaggt caacaaagaa
     3541 gtctttatag aacctgcagc agtctatagg aaatccgtga tatggcatct ctacattatg
     3601 agtatggagg attaaatatc ctcccgaatt taacgcgcca accatatttt ctattgctat
     3661 agatggcctg cagatatgtt ctaacaatgc ctgagagaat acaaaattat attttcttaa
     3721 ccctcttgct atcaatagcg aatcttgtat attcagatct acttcataat cttttccttt
     3781 ttcccctgag tatccaagag tatcaatttt actcagttta aatttctctt taaatacagg
     3841 taatacttct ttaggctctg ctacaagcag aagactatct cctgttttac tatatttctc
     3901 catatatcca aataaaacag accagaattt attactgctg ttcggagcag gtataaaatg
     3961 ttctggcccg taaaggtgtt taaagttata catctttaat cgtatattcc cctttcatct
     4021 tctttaatct ccggcctttt attttctctc accaaccatg cccattcttc aaattcagtg
     4081 cttccataag cgagtgtcag gcatagtctt attacttcga tcatgaataa gagtatccat
     4141 aaccatccac aaaaaaaaca gcatggcat
//
LOCUS       DAOQWT010000069         4157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_21890_length_4157_cov_3.364700, whole genome shotgun sequence.
ACCESSION   DAOQWT010000069 DAOQWT010000000
VERSION     DAOQWT010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21890_length_4157_cov_3.364700"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            395..1093
                     /locus_tag="PKJ95_02205"
     CDS             395..1093
                     /locus_tag="PKJ95_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65087.1"
                     /translation="MAIKKENICNEKEQTLSEKELVSKYQYLVTKIARQFKDIPESPE
                     ILEEVGYLGLLNAIHLYDNRIHHVEFKTYAQILITEEIHQYLLNRNRQIDYPDWLIEL
                     NEKVNDFVIKYREDYQRFPQISEIASHFNITDSGLQELLKARDSLRETCFPLYGESQY
                     DISSLKPDLTKIKSRSYQSFKLPIEDLIILRRAFKRIKEIKEGIIYYLFVMDLNQTKL
                     AQMLGMPPEKAKAN"
     gene            1199..2869
                     /locus_tag="PKJ95_02210"
     CDS             1199..2869
                     /locus_tag="PKJ95_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNR65088.1"
                     /translation="MITNKLFNFPKIIFENIFSSANRVWIELISNLFLVLIAAIITIA
                     IILYKQRKALKKIDVLSKIENELSNNFLNELPVYFVALNADGTTRYMNRTMLNSLGYQ
                     EKDVLQKDYFQLFIPSGERELLKGKFFNLSKKDNPSYNENIVLTKEGKKLWVKWYLQP
                     VYNSFGEFEFYNSIGIDLTAERRAKLEVENLFIPKNEQINLSTVKEKITAGLFFEKSF
                     SLKLKQVLKLLPRILPNDGSDIALLENSHLRVIAIDGYKGRYVQKIVRNLNTDINNFP
                     LEKEVIEKQKLVVIPDTYQDSRWRIIPGLEWIHSCIKVPIVIEGKILGTMGIASETRN
                     TFDQTSIKKIESFIYGFTASIYNYLNYYQLLKSRDDIIHAVTKLVETRDPYTVGHQEN
                     VARIATSIAREMGLKEDMVETIRIASLVHDIGKFNIPSEILNKPSRLNDIEYSLVKNH
                     SQLGYDILKDISFTTPIAEIVYQHHEKFDGSGYPRALKGEEILLEARIICVADVFEAM
                     ASHRPYRQNLGIEAAEEELKKNKGILYDPQVVDACLRIKEKTKALFNH"
     gene            3200..>4157
                     /locus_tag="PKJ95_02215"
     CDS             3200..>4157
                     /locus_tag="PKJ95_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNR65089.1"
                     /translation="MKMNQRKKGPKLSKKSILRNHNPKNNSHKDEESNLSSQNKLKKL
                     EEFFLQGREEFLNLFKRCPVALAYTNIDGIILQINHCFEELTGFTEDGVKGNSLVYCL
                     RPEEASYFDPNYSRTSFETNIYTKDNCAVQVLGNRKYNKIDNRLAGMIFSFQDISFLR
                     KERRIKQVLYRISQSVHSELPLPEIYAQAQEQLKKVIIAENFYIALWDSDTKQLHFPY
                     YTDEAAGDNEIFIQRYCSSQSIFHYILNNVKPVLMDFQRYRKMLSYGYIEPWDVMTNT
                     HLWLAVPIKDGQKAIGVLALQSYNNARQYSEKDIDLLEFVAEQ"
BASE COUNT         1478 a          670 c          755 g         1254 t
ORIGIN      
        1 aaagagaaga atgtgagtga cagagtatga gattgccacg acatgctttc agcatgtctc
       61 gcaatgacag gaggagatgt gggttcgcaa tgacaaaaac cattgctcct ctctctgtca
      121 ttgtgaggaa cgaagtgacg aagcaatcac atccactgta gcccgaataa gaaaataaca
      181 aacaaacagc gatgagtgat gtgctaacgg tatgaggata tgagtggtaa ataatcattg
      241 aagatttaaa agaaaaatag gttaaaataa aaaatttaaa aatactaaat actaacgacc
      301 aacgactttt gacacagtat ctatttaatg gcatactatc cagagcatag tttctaaatg
      361 taaatatcca tccagatgaa tggaggtaat tgctatggct atcaaaaaag aaaatatatg
      421 taatgaaaaa gaacaaacat tatctgaaaa ggaacttgtc agcaaatacc aatatttagt
      481 gactaaaatt gccaggcagt ttaaggatat tccggaatca cctgaaattt tagaagaagt
      541 cggttatctg ggtcttttga atgccatcca tctctatgat aatagaatac accatgtgga
      601 atttaagacc tatgcccaga ttctgattac cgaagagatt catcaatacc tcttaaacag
      661 aaatcgccaa atagactatc ccgattggct gatagagcta aatgaaaagg ttaatgattt
      721 tgtaattaaa tatcgcgagg attatcaacg tttcccccag atttctgaaa ttgcctctca
      781 ctttaatatc accgatagtg gtttgcagga attgctgaaa gctcgagatt ccctgagaga
      841 gacctgcttt cccctttatg gagaatcgca atatgatatt tcctcattaa aacctgacct
      901 gaccaaaatt aaaagtcgga gctatcaatc ctttaagcta cccatagaag atttaatcat
      961 cttaagaagg gcttttaaaa gaattaaaga aattaaagaa ggtatcatct actatctttt
     1021 tgtgatggat ttaaatcaga ctaaattagc tcagatgttg ggaatgcctc ctgaaaaagc
     1081 aaaggcaaat taaggtggat tgtatgtctg aagagaagaa gtatttgcaa aacattggac
     1141 gagaacaagg taaaattcta tattttttaa tagggatatt ttcgttgctt gtttttttat
     1201 gataactaac aagctattta atttccctaa aattatattt gagaatattt tttcttcagc
     1261 caatagagta tggattgaat taatctctaa tctgttcctg gtcttaattg ctgccattat
     1321 aactattgcc attatcctct ataaacaacg aaaggcacta aaaaaaatag atgtattatc
     1381 taagatagaa aatgaactat caaataattt tcttaatgaa ttgccggtat attttgtggc
     1441 actcaatgca gatggaacaa cacgttatat gaataggact atgcttaata gcttaggata
     1501 tcaggaaaaa gatgttttgc aaaaagatta ttttcagctc tttattccat caggggaacg
     1561 tgagctattg aagggaaaat tttttaattt atctaaaaaa gacaaccctt cttataatga
     1621 aaatatagtg ttgactaaag aagggaaaaa actatgggta aaatggtatt tacaacccgt
     1681 ttacaatagt tttggtgaat ttgagttcta taattctatt ggaattgatc tgacggcgga
     1741 aagaagagca aaactggagg tagaaaacct atttattccc aaaaatgagc agataaacct
     1801 gtctactgta aaagaaaaaa taaccgctgg tttatttttt gaaaaaagtt tttctttaaa
     1861 actaaaacag gtattaaaac ttttgcccag aatattgccc aatgatggtt ctgatatagc
     1921 tttattggaa aatagtcacc tgcgggtcat agctatagat ggatataagg ggaggtatgt
     1981 ccaaaaaatt gttcggaatc tcaatacaga tataaacaac tttccactgg aaaaagaagt
     2041 tattgaaaaa caaaaattag tggtaatacc tgatacctat caggattccc gctggagaat
     2101 aattcccggc ctggaatgga ttcattcctg tattaaagtt cccattgtta ttgagggaaa
     2161 aattctaggc actatgggta ttgccagtga aaccagaaat acctttgatc agaccagtat
     2221 aaaaaagata gaaagtttta tctatggttt cacagctagt atttataatt atttaaacta
     2281 ttaccagttg ctgaagagca gggatgatat tatccatgcc gttaccaaat tggttgaaac
     2341 cagggacccc tatactgttg gccatcagga aaatgttgcc cgtatagcta cttctattgc
     2401 ccgggagatg gggttaaaag aggatatggt tgaaacaatc aggattgcct ctttagtcca
     2461 tgatatcggt aaattcaata taccttctga aatattgaac aaacccagcc gcttgaacga
     2521 tattgaatat agtttagtaa aaaatcattc ccagctgggt tatgatatct taaaagacat
     2581 ctcttttacc acacccatag ccgaaattgt ctaccaacat catgaaaaat ttgatggcag
     2641 tggctaccct cgtgctttaa aaggggaaga gattctatta gaagcgcgta ttatctgtgt
     2701 ggcagacgta tttgaagcaa tggcttctca tcgtccttat cggcaaaacc tgggaattga
     2761 agcagccgaa gaagagttga aaaagaataa aggcattctc tatgaccctc aagtagtaga
     2821 tgcctgtttg agaataaagg agaaaaccaa agctttattt aaccattaat tttaataatt
     2881 ataataattc caatataaga tttgccaacc cttcttttaa ttctttatct aataaaaaag
     2941 taaaaaattc catctttacc tattcttgtg ataaaatata aaacaaagta catatttttt
     3001 atcagaattc acccattttt actttaatag aaacattagt tttttataaa tttttatttc
     3061 gaatcatctt ctttgtaatg aaactcagtc tgaattttag ataatgatat ttatagacaa
     3121 tatttctatt ataaaaaatg tttttgtaaa taggaaacca ataagagatt ctttaaaata
     3181 gttcacaata gaagtaacaa tgaaaatgaa ccagagaaaa aaaggcccta agctttccaa
     3241 aaaaagtata ctgcgaaatc ataaccctaa aaataacagt cataaagatg aagagtccaa
     3301 tttatcatca caaaataaat taaaaaaatt ggaagagttc tttctgcagg gtcgggaaga
     3361 gttcctcaat ctatttaaaa gatgtcctgt ggcgcttgcc tacaccaata ttgatggtat
     3421 tatcctgcag attaatcatt gttttgaaga gcttaccgga tttactgaag atggagtaaa
     3481 aggcaattct ttagtttatt gccttaggcc ggaagaggcc tcctattttg acccgaatta
     3541 cagcagaaca tcttttgaaa caaatatcta tactaaagat aactgtgccg ttcaggttct
     3601 gggtaacaga aaatataata agattgataa ccgcctggca ggaatgattt tttcttttca
     3661 ggacatctcc tttttgagga aggaaagaag aataaaacag gttctatatc gtatatcaca
     3721 gtcggttcat tctgaattgc ctttaccgga aatatacgcg caggcccagg agcaattaaa
     3781 aaaagtaatt atagcagaaa atttctatat tgcactatgg gatagtgata cgaagcagct
     3841 tcactttccc tattataccg atgaagctgc cggtgacaat gagatattta tacagcgcta
     3901 ctgttcatca cagagcattt ttcattatat tttaaataat gttaaaccgg tattgatgga
     3961 ttttcaacgt tatcgtaaga tgttgtccta tgggtatatt gaaccctggg atgtaatgac
     4021 caatacccat ctctggctgg cagttcccat caaggacgga cagaaagcaa tcggggtatt
     4081 agctttacaa agttacaata atgcccgaca atacagtgag aaagatattg atttgctgga
     4141 atttgttgcc gagcagc
//
LOCUS       DAOQWT010000070         4143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_21974_length_4143_cov_2.881849, whole genome shotgun sequence.
ACCESSION   DAOQWT010000070 DAOQWT010000000
VERSION     DAOQWT010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21974_length_4143_cov_2.881849"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKJ95_02220"
     CDS             complement(<1..189)
                     /locus_tag="PKJ95_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012280438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNR65090.1"
                     /translation="MEKNYFFTSESVTEGHPDKIADQISDAILDDLIGQDSEARVAVE
                     TLVATGLVLVAGQITTTGY"
     gene            complement(221..1174)
                     /locus_tag="PKJ95_02225"
     CDS             complement(221..1174)
                     /locus_tag="PKJ95_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNR65091.1"
                     /translation="MNNSKEDNKNIPKVMNKNNKEEMKSEKGKSKKNYFVNRNLNTVV
                     LLFLVAMTLLSLYSLTKNEQLYQSLSALNQSYNEIKEENQIFKEKIEEQSQKLDSINT
                     QMAKYLKERITKEQFTEIYMQMQELIGMVTVEQKREYYLSRIVNIISNNNKQLESETI
                     YDIAKNIYEIALKYKFNPFLICALIKVESDFKTDSISDSYAYGLCQVRRFIAKELAEN
                     LGIDWDGAEKTLLNPEKNIRIGIHYLSMLYDDFGDIKLALTAYNYGPFKVQEFINEDN
                     NIPNGYTEKILKYYARYRGFDIEEVDEILNGDNNESNILQG"
     gene            complement(1234..3192)
                     /gene="metG"
                     /locus_tag="PKJ95_02230"
     CDS             complement(1234..3192)
                     /gene="metG"
                     /locus_tag="PKJ95_02230"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNR65092.1"
                     /translation="MEKKKAFYITTPIYYVNDVPHIGHAYTTIAADTMARFMRLRGFD
                     VLFATGTDEHGQKIAKSAEQMGIKPLELADRVVIQFQNLWSVLNIKPDDFIRTTEKRH
                     ELVVQKIFNRLYEKGEIYKGEYEGWYCVPCETFWPESHIENNVCPDCGRPVEKFKEES
                     YFFRMSNYQERLLQHIDEHPDCIKPESRKNEIISFVKRGLKDQSVTRIKSSLNWGIDV
                     PFDKNHVIYVWFDALINYLTVAGYGIDDKKFNLYWPEAVHLIGKDILRFHTIIWFTML
                     MAADIAPPKMVFSHGWWTSEGEKMSKSRGNVIDPFQVAGEFGTDAFRYFLLRELSFGL
                     DGNFSRQLLIQRINYDLANDLGNLLSRTVAMTVKFYDGTIPVPSHSDELYDKELQNLA
                     EEVVAQVIVHMEQFSFNLALENIWSLIKRTNKYIDQTEPWLLGRDKNKKKRLEQVLYH
                     LNESLRITAMLLYPFMPATSDKIWQQLGIKNKIEDMTIPEDVSWGKLEPGSKVNPGDN
                     LFPRIEEEKKNIANAVEVNSKNIDKKNEGFEMESQQLPTISLTDFQKIDLRVGKIISV
                     EDVPGASKLLKIKVDFKSEIRTIVAGIKEFYLPEKLIGKSIVVVFNLAPATIRGIQSQ
                     GMLLAASEGQQVVLLTTDQEIEPGSRIR"
     gene            complement(3302..>4143)
                     /gene="rsmI"
                     /locus_tag="PKJ95_02235"
     CDS             complement(3302..>4143)
                     /gene="rsmI"
                     /locus_tag="PKJ95_02235"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNR65093.1"
                     /translation="RCLAILKKVDLIAAEDTRHSKILLNKYQIKKPVISYHKYSNEKK
                     VEYILQLLQEGKSIALLSDAGMPGICDPGFEIINKAIENDISIVPIPGVSAVTTALVV
                     SGFTMQKFVFEGFVPRKKGEKKRFFFNLKDEERTIVFYEVPHRIKDTLIIMEGIMGER
                     RVVITRELTKKFEEIIRGNLSQVVQVLDEQEIKGEITLVLEGNRVIKKEEVPFCEQDS
                     VRKDELEKDIKNYLLQGYHSKDIVSLVTKGYHISKKWAYEKVLELKQDNSWTSTKDEG
                     ISV"
BASE COUNT         1180 a          898 c          616 g         1449 t
ORIGIN      
        1 gtaaccggtc gttgttatct ggccggcaac taatactaaa ccagtagcaa ccagcgtttc
       61 aacggcgact cttgcttcac tatcctgacc gatgaggtca tctaatatgg catctgatat
      121 ctgatcggca atcttatcgg ggtgcccctc ggtaacagac tcagaagtaa aaaaataatt
      181 tttttccaat ttaccagcct cctaaaatat gtttattata ttagccctgc aaaatattag
      241 actcattatt atctccgtta agaatttcat caacttcttc tatatcaaaa cctctatacc
      301 tggcataata tttaagtatc ttctcagtat aaccattggg aatattatta tcctcattta
      361 taaattcctg aactttgaat ggtccataat tataggcagt taaagccagt tttatatcac
      421 caaaatcatc gtaaagcata gataaataat gtattccaat ccgaatattt ttttcaggat
      481 tcaagagggt tttttcagcc ccatcccaat caataccaag attttctgcc aattctttgg
      541 ctataaaccg gcgaacctgg catagcccat aggcatagga atcggagatg gaatcagttt
      601 taaaatcact ctccacttta atcagagcac agattaaaaa tggattaaac ttatatttta
      661 gcgcaatctc ataaatattt ttggcaatat catagattgt ttcactttcc agctgtttgt
      721 tgttgttaga aataatgttt acaatacggc tcagataata ctcccttttc tgctccacag
      781 ttaccatccc aatcagttcc tgcatctgca tataaatctc agtaaactgt tctttagtaa
      841 tcctctcttt caggtattta gccatctggg tattgatact gtccaacttc tggctttgct
      901 cctcaatctt ttctttaaat atctggttct cttcctttat ttcattatag gactggttta
      961 aagcagaaag agattgatag agctgttcat tttttgtcag actatacaat gataataggg
     1021 tcatcgccac cagaaacagg agcactaccg tatttaaatt tctattgaca aaataattct
     1081 ttttactttt tcccttttca gacttcattt cctctttatt atttttattc atcacctttg
     1141 ggatattttt attatcctct ttactattat tcatagtatt atttatcctt aatattttct
     1201 actctaaatt tatgagcaga agtccttcta attttacctt attctgctac ccggttcgat
     1261 ttcctgatca gtggtaagaa gaactacctg ctgcccctct gatgctgcca gaagcatacc
     1321 ctgtgattgt atacctctga tggtggccgg agctaaatta aatacgacta caatactttt
     1381 accaattaat ttttccggta agtaaaattc ttttataccg gcaactatag ttctaatttc
     1441 agatttaaaa tcaactttta tttttaacaa cttgctggca ccggggacat cttcaaccga
     1501 aattattttg cccaccctga gatcaatttt ttgaaaatct gttaaagaaa ttgtgggtaa
     1561 ctgctgactt tccatctcaa acccctcatt tttcttatct atattcttgc tattcacttc
     1621 aactgcattg gcaatatttt tcttctcctc ttcaattctg ggaaacagat tgtccccggg
     1681 atttactttg cttcccggct ccagcttccc ccagctaaca tcttcaggaa tggtcatatc
     1741 ttcaatctta ttttttatgc ccagctgctg ccaaatctta tctgatgttg cgggcataaa
     1801 gggatataac agcatagctg ttattcgcaa agactcattg aggtggtata agacctgttc
     1861 cagtcttttt ttcttatttt tatccctgcc caaaagccag ggttcagtct ggtctatata
     1921 tttattggtt cttttaatca gggaccagat attttccaaa gcaaggttaa aggagaattg
     1981 ttccatatga actattacct gtgctactac ttcttcggcc aggttttgca attctttatc
     2041 ataaagttcg tcactatgag aggggacagg aattgttcca tcataaaatt ttacagtcat
     2101 ggccacagta cggctgagca ggttgcccag atcattggct aaatcgtaat taatgcgctg
     2161 gataagcaac tggcgggaaa aatttccatc cagtccaaag gagagttctc taagtaaaaa
     2221 ataacgaaag gcgtcagtcc caaattcacc cgctacctga aaggggtcaa ttacatttcc
     2281 ccttgatttg gacatttttt cgccttcact ggtccaccag ccatgggaaa aaaccatttt
     2341 aggcggcgct atatcagcag ccatcagcat agtaaaccag ataatggtat gaaatctaag
     2401 tatatcttta ccgattaagt gtactgcctc aggccagtac aggttgaact ttttatcatc
     2461 aataccatat ccagcaacag tgagataatt tatcaaggcg tcaaaccaga cataaattac
     2521 atggttttta tcaaaaggaa catctatacc ccagttcaga ctggatttaa tcctggttac
     2581 actctggtct tttagccctc tttttacaaa actgataatt tcattctttc tcgattcggg
     2641 tttaatgcaa tcgggatgtt catcgatatg ttgcaataat ctttcctgat aattgctcat
     2701 tctgaaaaag taactttctt ctttgaattt ctcaaccggt ctaccgcaat cagggcatac
     2761 attgttttct atatggcttt ccggccagaa agtttcacag ggcacacaat accatccttc
     2821 atattccccc ttataaatct cacctttttc atataaacgg ttaaatatct tttgcaccac
     2881 cagttcatgg cgtttttctg tggtgcgaat aaaatcatcc ggtttaatat ttaagacaga
     2941 ccagagattc tgaaattgta ttaccacccg gtcagctagt tccaggggtt ttattcccat
     3001 ctgttctgct gatttggcaa ttttctgacc gtgttcatca gtacctgtag caaaaaggac
     3061 atcaaagcct ctcagtctca taaacctggc catagtatct gctgcaatgg tagtataggc
     3121 atgcccgata tgagggacat cattgacata ataaatagga gtggttatat aaaatgcttt
     3181 ctttttttcc atttttctac tttaaatcct ttccctttcc cttgttaatc tgtatttcca
     3241 atcaacagta tctattgaac agttgggttt tataccagta aaaatcccta aattttttta
     3301 gttaaacaga aatgccttca tctttagtag aagtccagga attatcctgt tttaattcta
     3361 ataccttttc atacgcccat tttttagata tatgataccc tttagtaact aaagatacaa
     3421 tatctttgct atgatatcct tgaagcaaat aattttttat atctttttcc aattcatctt
     3481 ttctgacaga atcttgttca caaaagggaa cttcctcttt cttaataact ctatttcctt
     3541 ccagaaccag agtaatctct cctttaattt cctgttcatc caaaacctgc actacctggg
     3601 aaaggtttcc tctgattatc tcctcaaact tcttagtcaa ctcccgagtt atgaccaccc
     3661 ttctctcccc cattatccct tccataatta ttaaggtatc tttaattctg tgagggactt
     3721 cataaaatac aatagtcctc tcctcatctt ttaaattaaa aaagaacctc tttttttctc
     3781 cttttttcct gggaacaaac ccctcaaaaa caaacttctg catagtaaat cccgatacta
     3841 ccaatgccgt ggttacagct gaaacaccgg gtatggggac aatagatatg tcattttcaa
     3901 tcgctttatt gattatttca aaccccgggt cacaaatacc gggcattccg gcatctgata
     3961 ataaagcaat acttttcccc tcttgtaaaa gttgtaaaat atattccact ttcttttcat
     4021 tgctatattt atgataactt ataaccggtt ttttaatctg atatttattt aacaaaattt
     4081 tgctatgtct ggtatcttca gctgcaatca aatccacttt ttttaaaatt gccagacaac
     4141 gca
//
LOCUS       DAOQWT010000071         4135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_22032_length_4135_cov_3.857843, whole genome shotgun sequence.
ACCESSION   DAOQWT010000071 DAOQWT010000000
VERSION     DAOQWT010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22032_length_4135_cov_3.857843"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKJ95_02240"
     CDS             <1..447
                     /locus_tag="PKJ95_02240"
                     /inference="COORDINATES: protein motif:HMM:NF020742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNR65094.1"
                     /translation="TPGWKFNEWEMKGVPVRLEIGPRDLAQNQVVAVRRDKGNKVIIS
                     EKEVESTLEKWMADIQNNLFLQARSRLEENIRFTDNYESFKEIMEEKRGFIKAFWCGN
                     EKCEDQIKKDSKATIRCIPLEEHKEKGKCIFCGEESSSLVYFGRAY"
     gene            541..798
                     /locus_tag="PKJ95_02245"
     CDS             541..798
                     /locus_tag="PKJ95_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor-like protein"
                     /protein_id="HNR65095.1"
                     /translation="MEKVLIGHIVDYFAKIGVAAIEIDSGEIRVGDTVYFSGHTTDFE
                     QEIDSMQIDRNSINSAQAGDCIGIKVRERVREGDEVYKIIS"
     gene            835..1944
                     /locus_tag="PKJ95_02250"
     CDS             835..1944
                     /locus_tag="PKJ95_02250"
                     /inference="COORDINATES: protein motif:HMM:NF027889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HNR65096.1"
                     /translation="MVNSNICNDELFMPGDWLPVIQKIQKHEGTVIVLGKTDSGKTSF
                     IKLLTCFLVRRNRKIGIIDSDIGQSTLGPPATIGLSIADKNRLKDGIIPVDYIYFIGA
                     ISPEQCIDRFLDRIYRLYTIAEKEKIDVLLIDTTGLVMGRVGQYLKGNLIRKIHPSAL
                     VALQFDRELEPILSEFVSQSLIHTYRITPYPNIVERNWRDRKSRREKQFNLYFKGLQV
                     KEIDFSTMVMKDFNFGLSFCSDNNIAESIGQIFKLEIIFLEVIGSRAILILPDPQNIP
                     DKATFIKIKRYLQVEQVVLIFPHWFQNILASLNNAEGLSIGLGIVKRIDVAEKRNTFL
                     VPASLSLGNIAQIELGKVRIKPDGTELPYREPLKY"
     gene            1974..2873
                     /locus_tag="PKJ95_02255"
     CDS             1974..2873
                     /locus_tag="PKJ95_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNR65097.1"
                     /translation="MDKKEREGVILVIICALIHGTHPILGKYGVSLVSPLFFASLTNL
                     IAAAGLIFVILFKKEPLVKLINKRYFLLLILIGFFGTTLSNILFFYGVRLTSGINSAI
                     LLQVEPIYALVIGYLLLKERITIRQIFSTLLILFGTLLVIYQGTSRLNWGDILILLTP
                     LCYQTGHYFSKRLLNKTELNPLFLAAGRTFYGGTILFFLYQCIDANEFNTLTHPDILG
                     IFIFFGIVVYALSYITFYGAIKRINLSKASAIISIYPAISIILARFILKEVPDFYQLS
                     GFFIIMVGIFYLSNLKSELREQE"
     gene            3126..>4135
                     /locus_tag="PKJ95_02260"
     CDS             3126..>4135
                     /locus_tag="PKJ95_02260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR65098.1"
                     /translation="MENTLNNSPESKKNTARDYNLIRILIALFLPYLALSVAQNGFLG
                     LLPFIRDEFDLSRVQVGYYSTFFFISASFLSVFSGNIVDSFGPKKSILLGIGSLGILL
                     SLQGLSSLYSVILILSFLSGLGFSIITPSATKAVMLATPREKRAFSMGFTQSGFGMGG
                     ILGASILPFLGEKMGWRAAVQIAAGAVLLTGLWVYKLYHDNENDKNTIHKPEDKKSKK
                     DTFREHLSFIIADKLLFRICFSGMLFGISEGALLAHFVVFLTDDLMISKVAAGLNFAA
                     LHLGGMAGLLGWGFFSDRFFRIDRRFGIFLIGLSSGFIYLFFGLFVCRPFLNQILVFF
                     LS"
BASE COUNT         1297 a          642 c          835 g         1361 t
ORIGIN      
        1 actccggggt ggaagttcaa cgagtgggaa atgaagggag ttccggttcg attggaaatt
       61 ggaccccgtg atcttgctca gaatcaggtg gttgcagtca gaagagataa agggaataaa
      121 gtaataatat ctgagaaaga agttgaatca actctggaaa agtggatggc tgatattcaa
      181 aacaacttat ttcttcaagc caggtcacga ttggaagaaa atatcaggtt taccgacaat
      241 tatgaaagtt ttaaagagat aatggaagaa aaacgcgggt ttataaaagc tttttggtgt
      301 ggtaatgaaa aatgtgaaga ccagattaaa aaagacagca aagcaactat tcgctgtatt
      361 ccacttgaag agcataagga aaaggggaaa tgtattttct gtggtgaaga gtcttcctct
      421 ctggtttatt ttggcagagc ttattgattt atgccagggt tgggacaggc aaaaatagag
      481 gttctgtttt tttatttttg aaataagaaa aaaatttaat aagggaagga gggtattaag
      541 atggaaaagg tgttaatcgg acatatagtt gattattttg ccaaaatcgg tgttgcggct
      601 attgaaattg attccgggga aataagggta ggagataccg tgtactttag tgggcataca
      661 accgactttg aacaggaaat tgattcaatg cagattgaca gaaactctat taattctgct
      721 caagccggag actgtattgg tattaaagta agggagagag taagagaagg agatgaagta
      781 tacaagataa tctcttaaca cctgtctttg taaattcagt ttaggaaaat aataatggtt
      841 aactctaata tctgcaatga tgagttgttt atgcctggag attggctacc ggtaattcaa
      901 aagatacaga aacatgaagg aacggtaata gttttaggaa aaacagattc cgggaaaacc
      961 agttttatta aactattaac ctgttttcta gtaagaagaa atcgtaaaat tggaataatt
     1021 gattcagata tcgggcaatc aactcttggc cctcctgcta ccatcggact gagtattgct
     1081 gataaaaata gattaaaaga tggtataatc cctgtagatt atatatactt cattggtgcc
     1141 atatctcctg agcaatgtat cgatagattt ctggacagga tttacagact ttacactatt
     1201 gctgaaaagg aaaaaattga tgtcctgtta attgatacca caggtttggt aatggggagg
     1261 gtcggacaat acttaaaagg aaatttaatc aggaaaattc accccagcgc cctggtggcg
     1321 ttacaatttg acagggagtt agaaccaatc ttatccgaat ttgtctctca atctctcatc
     1381 catacttatc gtattacacc atatcccaat attgttgaaa gaaactggag ggacagaaag
     1441 tcaaggaggg aaaaacagtt taacctctat tttaagggtt tacaagttaa agagattgat
     1501 ttttcaacta tggtaatgaa ggattttaac tttggtctca gtttttgttc agataataat
     1561 atagctgaat caatcgggca aatctttaaa ctggaaataa tatttttaga agtaattgga
     1621 agcagagcca ttctaatttt accggatcca caaaatatcc ccgataaagc cacttttatt
     1681 aaaattaaga ggtatcttca ggtagaacag gttgtcttga tttttcctca ttggtttcaa
     1741 aatatactgg cttcccttaa taatgcagag ggactcagta tcggtttagg aatagtaaaa
     1801 aggattgatg tagcggagaa aaggaatact tttttggtac ccgcctctct ttcgctggga
     1861 aacattgctc aaatagaact aggaaaggtg agaatcaaac ctgatggtac agaattgcct
     1921 tatagggaac cactaaaata ttagttcatt acaaaatgca gaaagagaag attatggaca
     1981 aaaaagaacg ggaaggggtt atactggtaa ttatatgtgc tttaattcat ggcacccatc
     2041 ccatattggg taagtatggt gtttcattgg tgtccccgct tttttttgcc agtttaacca
     2101 accttattgc tgcagcaggt ttaatttttg tcatcttatt taaaaaagaa ccactggtta
     2161 agttaattaa taaaaggtat tttcttttat taattttgat tggatttttt ggaacaactc
     2221 tgtccaacat tctgtttttt tatggtgtca ggttaaccag cggcattaac tccgctattt
     2281 tattacaggt agaacccatt tatgccctgg taatagggta tctccttctt aaagaaagaa
     2341 taaccataag acagattttt tccactttac ttatcctttt tggcaccctg ctggttattt
     2401 atcagggaac ttcccgctta aattggggtg atattttgat tctgctaacc cctctctgct
     2461 accagaccgg tcattatttt agcaagcgat tactgaataa aactgagcta aaccctcttt
     2521 ttcttgccgc aggaaggact ttttatggcg gaaccatctt gttttttctc tatcagtgta
     2581 ttgatgcgaa tgagtttaat acactgactc atccagatat tcttggcatt tttatctttt
     2641 ttggcatcgt ggtatatgca ttaagttata taacctttta tggagctata aaacgaatta
     2701 atctttccaa agccagtgct ataatttcaa tttatcctgc gatatctata atcctggctc
     2761 gctttatctt aaaagaagtg cctgattttt atcaactgag tggatttttc atcataatgg
     2821 tgggcatctt ctatctatcg aatttgaaga gtgaattgcg ggaacaggaa tgatttattc
     2881 ttaaatactc ttactcaaga atgctcctaa ttcttaatct aatacattac tatgcaaaaa
     2941 tataattccc cgtaacaaat gtattgaata tgttagaagg ataaatagat aaaaaaatag
     3001 gagaaaaatt tgcctttcat tactatgatg atagaataac aaagaaaggg agacgttgta
     3061 tatttattag aaaatttagg gataaaaata aaattataaa ataaaaatac ataaataata
     3121 aaactatgga aaacacacta aataattctc ctgagagcaa aaaaaatact gccagagact
     3181 ataatcttat aagaatattg attgcacttt ttctccccta tttggcctta agtgtagcac
     3241 agaacggttt tttgggatta cttcctttta ttcgtgatga gtttgacctt tcacgtgttc
     3301 aagttggata ttattccact ttctttttta tcagtgcatc atttctttct gtattcagcg
     3361 gtaatattgt agattctttt ggaccaaaaa agagtatact tttaggaatt ggaagtctgg
     3421 ggattttact atcactccag ggactttctt ctctctatag tgttatttta atattatcct
     3481 ttttgtccgg attagggttc agcattatta caccatctgc cacaaaggca gttatgcttg
     3541 ccactcctcg ggaaaagaga gctttttcaa tgggatttac ccagtcaggg tttggcatgg
     3601 gggggatcct gggagcaagc attctgcctt ttttagggga aaaaatgggc tggagagctg
     3661 ctgttcagat tgctgcaggg gccgttctgt taactggctt atgggtgtac aaactatatc
     3721 atgataatga aaatgataaa aacacaattc ataagcctga agataaaaaa agtaaaaaag
     3781 atacctttcg agagcatctc tctttcatca tagcagataa gcttttattc aggatttgct
     3841 tttcgggaat gttgtttggc atttcagaag gagctctact tgcccatttt gtggtatttt
     3901 taactgatga tctaatgata agtaaggtag ctgctggttt gaactttgca gctcttcatc
     3961 ttggaggaat ggccgggctt ttagggtggg gattcttctc tgaccgattt tttagaatag
     4021 accggcgttt cggtattttc ctgattggat tatcttcagg atttatatat ttatttttcg
     4081 gtctttttgt atgccgccct tttttaaatc agattttagt ttttttcctt tcttt
//
LOCUS       DAOQWT010000072         4111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_22194_length_4111_cov_3.087525, whole genome shotgun sequence.
ACCESSION   DAOQWT010000072 DAOQWT010000000
VERSION     DAOQWT010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22194_length_4111_cov_3.087525"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PKJ95_02265"
     CDS             complement(<1..914)
                     /locus_tag="PKJ95_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR65099.1"
                     /translation="MNGKFQLISDYSPQGDQPEAIKKICQGYEEEKHIQTLLGVTGSG
                     KTYTMANVIQYLQMPTLVISHNKTLAAQLCNEFRRFFPNNAVGYFVSYYDYYQPEAYI
                     PRTDTYIEKDASINDEIDRLRLSATAALLERNDVIIVSSVSCIYGLGSPDDFSQLVLS
                     VAVNQVIKREEIAKRLIEIQYERNDIEFQRGCFRIRGDILDIFPAYLENAFRVEFFGD
                     QIEAIHEINPLTGHKIEKKEKITIYPAKHFVTPQKRLERAITTIKEELGDRLDYFHQN
                     NKLLEAQRLEQRTKYDLEMLSQFGYCTG"
     gene            complement(1093..1722)
                     /gene="coaE"
                     /locus_tag="PKJ95_02270"
     CDS             complement(1093..1722)
                     /gene="coaE"
                     /locus_tag="PKJ95_02270"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206532.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNR65100.1"
                     /translation="MKILIVGLTGGIVSGKSTVAKMFKQLGAQIIDADKIARSIVQPG
                     EKAWQSIVENFGRGILSDNQELNRKELARIVFADKEKLETLNRITHPEITAIIKDKIE
                     RMRRKNITKESICIVEAPLLFEAHLEEMMDKIIVVYLNREEQIRRLLLRNNLTQQEAI
                     DRIESQMPLEEKVQRADYIIDNCTSINQTKKQVKQVWSELKQLLVSINK"
     gene            complement(1767..>4111)
                     /gene="polA"
                     /locus_tag="PKJ95_02275"
     CDS             complement(1767..>4111)
                     /gene="polA"
                     /locus_tag="PKJ95_02275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNR65101.1"
                     /translation="EADDLIGTLVHRIKESEYKPVIVTGDRDLLQLADEDTEILLMQK
                     GVTQVEQYGLHSIEKLLGFSPHLIPDYIGLKGDPSDNIPGVPGIGEKTAQRLIQQYGS
                     LEEIIANSHHIESKPIREKILQYVEQARLSKRLAIIKRDVDFKMELSQMAYSGPDYEK
                     LRALFGKYEFKKLLEKIPEQDRNIKKTKEGIIEFQEITDEEGLSRLCQKIKTRGVFSF
                     LEESYTSASSKIVEFAAIAIPEEVFLIIINNQDSIIDINRHLSIEQVSGKLNEIFQDE
                     EIEKIGFDLKNFWTCFSNDTHFFTSPYFDIKIASYLLNPSQENYQLNKIIEDNLIGKY
                     GTDIKFNEQGEFADRKKEICWQTKLLLSLKEKLWEQIYAQNMDKLYLNVELPLIKVIY
                     LMEKKGIKLDTAFLKKMSQQFSHKITELRNTIFDLAGEEFNLNSPKQLSYILFNKLKL
                     PTMKKIKTGYSTNAQVLLELSEKHEIVAQILDYRELEKLKNTYIDKLPRLINPQSGRI
                     HSTFNQTGTSTGRLSSNNPNLQNIPVRTELGKDIRNAFIADEGNIFLSADYSQIELRI
                     LAHLSHDEDLKKAFLEGKDIHSYTAAQLFNVDLNLVSEEMRRIAKTINFGIIYGMSSF
                     GLAANLRISREEAAQYIEAYFQRYHRVKEYIDEQIIIARKNKYVRTMLNRIRYLEGID
                     SSNKSIREFYERTAINTPIQGTAADLIKIAMVGINKRMERMNLSSGLILQIHDELIFE
                     IPEAELQKRQAIIKDIMENCLPLNIPLKVNFKSGYRWGEL"
BASE COUNT         1154 a          845 c          648 g         1464 t
ORIGIN      
        1 ataccggtac agtagccaaa ttgggagagc atctccagat catatttggt tctttgttct
       61 aatctttggg cttctaataa tttattgttc tgatggaagt aatccagtct atctcctaat
      121 tcctctttaa tggtagtaat ggccctttct aatctttttt gaggggtaac aaaatgtttc
      181 gccggatata tggtaatttt ttctttcttt tcaattttat gtccagttaa aggattaatt
      241 tcgtgaattg cctctatctg gtccccgaaa aattcaacgc ggaaggcatt ttccagatag
      301 gctggaaata tatccagaat atctcctctt attcggaaac aacctctttg aaattcaata
      361 tcattccgtt catactgaat ttctattaac cttttggcta tttcttccct ttttatcacc
      421 tgattcacag caacacttaa aaccagctgc gaaaaatcgt caggagaacc aagtccatag
      481 atacaggaaa cactggagac aataattaca tcatttcttt ccaacagagc agctgtggct
      541 gacaaacgca ggcgatctat ttcatcattt atggacgcat ctttttcaat ataggtgtcc
      601 gttcggggaa tataggcctc aggctgataa taatcataat aactgacaaa ataaccgact
      661 gcattgttag gaaaaaacct cctgaattca ttgcaaagct gggctgccag tgttttattg
      721 tgtgagataa ccagagtagg catttgcaaa tactggatga cattagccat agtataggtc
      781 ttccctgagc cggtaactcc cagcagagtc tggatatgct tctcctcctc atatccctga
      841 catatctttt ttattgcctc cggttgatct ccctgaggag aataatcaga aataagctga
      901 aattttccat tcatagcaca ggacatcctt tttcttcact gaatagcctt atctctgtaa
      961 gagatatatt ctgctaaaat aattcaactt attattatac cataccctgc aaacagggca
     1021 gtctattcaa aggagagatg aagggatgca gatattttta agcaggcatt ttttaactga
     1081 aaaaccggtt ttttatttat taatgctaac cagcaattgt ttcaattcag accatacttg
     1141 ctttacctgt tttttggtct ggttaataga ggtacagttg tcaatgatat aatctgcccg
     1201 ttgcactttc tcttctaggg gcatttgtga ttcaattctg tctatcgctt cttgctgagt
     1261 aagattattc ctaagaagga gccttctaat ctgttcttca cggttgagat ataccactat
     1321 tattttatcc atcatttctt ccagatgtgc ctcaaataat aagggcgcct caacaatgca
     1381 tattgattct ttagtaatat tttttcttct catccgttct attttatctt taatgattgc
     1441 cgtaatttca ggatgggtta ttctgtttaa cgtttccaat ttctctttgt cggcaaagac
     1501 aattctggct aattctttgc gattaagctc ctgattatca cttaatatcc ctctcccaaa
     1561 attctccaca atactttgcc aggctttctc tcccggttga acaatactgc gggcaatctt
     1621 atcagcatct attatttgtg cccccaactg tttaaacatt ttggcaacag tagatttccc
     1681 ggaaacaata ccccctgtca atcccacaat tagtattttc aagagactat gcctctcctt
     1741 ttaaagataa tctatccttc tcactattac agctctcccc agcgataacc acttttaaaa
     1801 tttactttta acggtatatt caggggcagg cagttttcca ttatatcttt gattatagcc
     1861 tgtcttttct gtagctctgc ctctggtatt tcgaagatta actcatcatg gatctgcaag
     1921 attagaccac tggatagatt cattctttcc atccttttat ttatcccgac catcgctatt
     1981 ttaattaaat ctgctgcagt accctgaatg ggggtattaa tagcagtacg ttcataaaat
     2041 tctcttattg atttattgga actatcgatt ccttccagat atcgtatcct gtttaacata
     2101 gttctgacat atttattctt tctggcaatg ataatctgtt catctatata ttctttaaca
     2161 cgatgatatc tctgaaaata ggcttcaata tattgtgctg cttcttctcg acttattctc
     2221 agattagccg ccagaccaaa gctgctcatt ccataaataa taccaaaatt aatggtcttg
     2281 gcaattctcc tcatctcttc acttaccaga ttcaaatcaa cattaaacaa ttgcgctgcg
     2341 gtataggaat gaatatcttt tccttctaaa aaagcttttt tcaaatcttc atcgtgggat
     2401 aaatgagcca gaattctaag ttcaatctga gaataatcgg cagataaaaa tatattgcct
     2461 tcatctgcta taaaagcatt ccttatatct ttacccaact ctgtccgaac aggaatattc
     2521 tgcaaattag gattattact gctcagcctt cccgttgaag taccggtttg attaaaagtg
     2581 ctgtgaatcc taccggactg aggattaatt aatcgaggaa gtttatcaat ataagtattt
     2641 tttaattttt ccagctcccg atagtccaga atttgtgcta ctatttcatg tttttcagat
     2701 aattccagca gtacctgcgc attagtagaa taacctgtct taattttttt catagtgggt
     2761 agttttaatt tattaaacag aatataactt aactgctttg gtgaattaag attaaattct
     2821 tctccggcta aatcaaagat ggtatttctg agctcagtaa tcttgtgaga aaactgctgg
     2881 gacatcttct tcaaaaaagc agtatccagc ttaatgccct ttttttccat aaggtagatt
     2941 acctttatga gaggaagttc cacattgaga tacagcttat ccatattttg ggcataaatt
     3001 tgttcccata atttttcttt taatgataat aataattttg tctgccagca tatttctttt
     3061 ttccggtcag caaactctcc ctgctcatta aacttgatat ctgtaccata cttcccaata
     3121 agattatcct ctattatttt gtttaactgg taattctcct gagaaggatt taataagtag
     3181 gaggcaatct tgatatcaaa ataaggagat gtgaaaaaat gagtatcatt agaaaaacag
     3241 gtccagaaat tcttcaggtc aaagcctatc ttttctatct cctcatcctg aaaaatttca
     3301 tttaattttc ctgagacctg ttctatagaa aggtgtctat ttatatctat tatagaatcc
     3361 tggttgttta ttatgattaa aaatacttct tccggaatgg ctatagcagc aaattctact
     3421 atttttgagg aagcagaggt gtaactctcc tctaaaaagg aaaaaactcc ccttgttttg
     3481 attttctggc acaatcttga tagcccttct tcatctgtaa tttcctggaa ttcaattatt
     3541 ccctcttttg tttttttaat gtttctatcc tgctcaggaa tcttctctaa caatttctta
     3601 aactcatatt ttccaaacag agctctcaat ttttcataat caggtccgga atatgccatt
     3661 tgggataatt ccatcttaaa atcaacatct cttttaatta tagctaatct cttactcaat
     3721 ctggcctgtt ctacatattg taatattttt tctctgatgg gcttactctc aatatggtga
     3781 gaattggcaa ttatttcttc cagactccca tattgctgga ttagcctttg agcggttttc
     3841 tctcctatac cgggtactcc cggtatatta tcagatggat cgcctttgag tcctatgtag
     3901 tccggaataa gatgagggga aaaacccagc aatttttcta tagagtgcag accatattgc
     3961 tcaacctggg ttactccctt ttgcatcagc aatatttcgg tatcttcatc agccagttgc
     4021 aatagatcgc ggtccccggt aacaataacc ggcttatact cgctttcctt aattctatga
     4081 accagggtac ctataagatc atcagcttcg t
//
LOCUS       DAOQWT010000073         4084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_22411_length_4084_cov_5.045421, whole genome shotgun sequence.
ACCESSION   DAOQWT010000073 DAOQWT010000000
VERSION     DAOQWT010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22411_length_4084_cov_5.045421"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1376)
                     /locus_tag="PKJ95_02280"
     CDS             complement(102..1376)
                     /locus_tag="PKJ95_02280"
                     /inference="COORDINATES: protein motif:HMM:NF037255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNR65102.1"
                     /translation="MSKKNNIEKQEDKKKQEKDEDKKLINNMFIRNQKYPETDMLFTF
                     QLKSLEDIKNDCYIVLDTNVLLAPYETSNKSLDVLKTIYKELIKNKKLIIPGQVVREF
                     VDNRPKKISDLYKSLSNNKNKKPSSLPDIKTVPLFENINEYEELIKVRKKLNEELEKH
                     WEKYKNAINKVLELIESWSLNDPISLVYKELFDKDVILDITSDKVDKNDLEWRKKNHL
                     PPGYKDSDKEDGGVGDYLIWYTILKIAKEKKQSVIFVTDEQKNDWWHKADKETLYPRF
                     ELIDEFRRFSEGHSFHIINLSRLLELLKIDKEIIQEFRELREKHEKYTVNMNNLKERL
                     GNATENFLGRELTPDLLESVFITLVPLKIEEFYTQELQKQFSEIIQEINTPPKRTELA
                     KDKEKAIKIAENLFDLYKRVFSIENTELELWV"
     gene            complement(1716..1889)
                     /locus_tag="PKJ95_02285"
     CDS             complement(1716..1889)
                     /locus_tag="PKJ95_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65103.1"
                     /translation="MGRRREYNNIQLFLIKISACCVMVQFYCVIGGIAILIIGTIIGL
                     ICVMLGITSEKGY"
     gene            2389..3012
                     /locus_tag="PKJ95_02290"
     CDS             2389..3012
                     /locus_tag="PKJ95_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65104.1"
                     /translation="MVKLTCNACGGKLEITDDIERFACGHCGNEWIVNRSGGIVSLKA
                     IEEQLSKVQEHTKETAEHSKILADQVRLKAIKEKITTLSIETNGIDKNPQKKNNIKYA
                     NPLKIGWIALLVFIISLCIRLLLDKLKIKNIFVDYILGLPTMLSLSIICLCIVGAIIN
                     SIYSSLGNNTCSDEQQKQININKIKELKNEINNLENKAKEIEAGFFK"
     gene            complement(3097..3825)
                     /locus_tag="PKJ95_02295"
     CDS             complement(3097..3825)
                     /locus_tag="PKJ95_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNR65105.1"
                     /translation="MKRVKEEPGQGEQPEQPGEGEQLQTGKYKSAIAEIKAELKSEFG
                     KLPADWSWTRDRKLPATLSREECQELITAYKKGKLAVRNNLIIRTLYATGLRAEELEN
                     LKFCDIFYDNKTVFVRAGKGDKDRYCAIDKGTLEQLKAWQEGKGLEESVFGITLRQIR
                     RVVEGAGDMTGISAKYEAMGRVFSTHSMRHAYATHCYENSMRLPTLQRFLGHEYILTT
                     MVYLYTAQKYDVLEYGRTHPLEKE"
BASE COUNT         1324 a          766 c          530 g         1464 t
ORIGIN      
        1 ggctctgagt gcggtgtttt gaaagggtat tttttgtgtg aacgtcctgt gttacattag
       61 cttatattgg aattacacag gatgacctgg atgatgttta attataccca tagttctaac
      121 tctgtatttt caatactaaa aactcttttg tataagtcaa atagattttc tgctattttt
      181 atggcctttt ctttatcttt tgctagttca gttcttttgg gtggtgtatt tatttcttga
      241 attatttctg aaaattgctt ttgtaattct tgtgtataaa attcttctat ttttaatgga
      301 actaaagtaa taaaaacact ttcaagaaga tcaggagtaa gctctcttcc taaaaaattt
      361 tctgtagcat tacctaatct ttcttttaaa ttattcatat taactgtgta tttctcgtgc
      421 ttttccctta gttctctaaa ttcttgtatt atttctttat ctatctttaa taactcaagt
      481 aaccttgata aatttataat atgaaatgag tgtccttcag aaaaacgtct aaactcatca
      541 attaactcaa atcgcggata taaagtttcc ttatctgctt tatgccacca atcatttttt
      601 tgttcatcag taacaaatat aacactttgc tttttctctt tggcgatttt taaaatagta
      661 taccatatta aataatctcc tactccaccg tcttctttat cagaatcttt ataacctgga
      721 ggaagatgat tttttttcct ccattctaaa tcattcttat caaccttatc actggttatg
      781 tctaaaataa catctttatc aaataattct ttataaacta aactaatagg atcatttaaa
      841 ctccaagatt ctatcagttc taaaacttta ttaatagcat ttttatactt ttcccaatgt
      901 ttttctagtt cttcattaag cttttttctt actttgataa gctcctcata ttcattaata
      961 ttttcaaata aaggtactgt cttaatatct ggcaaagaag atggtttttt atttttatta
     1021 tttgataaac ttttataaag atcagaaatc tttttaggtc tattatccac aaattctcga
     1081 acaacttgcc ctggaataat taatttttta ttcttaatga gttctttata aattgtcttt
     1141 aatacatcta aacttttatt acttgtttca tacggagcta aaagcacatt tgtatcgagg
     1201 actatataac agtcattctt tatatcttct aatgatttta attgaaaggt aaataacatg
     1261 tcagtttccg gatatttttg atttcttata aacatattat ttattaattt tttatcttca
     1321 tctttctctt gtttcttttt atcttcttgt ttttctatat tatttttttt agacatttta
     1381 taccacctct aacatttatt attccgaata tttaagcatc tataaagcat tcaacaaaat
     1441 tttttgtaaa tccttctctt tgaaagtttt gaacttatgt aattcgagcg aacccagaca
     1501 tagactgagt tttcagcata aaaccattaa acataaagtt taacccggat gaaaattcct
     1561 atgcattata tttaaacttg aatttataat ttagaggcaa taccatattt tatggagtta
     1621 tgcgggtttg agtgctcata gagatatgaa aaaatggctc tattactgga taaaatatct
     1681 taacgccaaa atctgtcgtt ttgatcccgg tacccctaat accctttttc acttgttata
     1741 cccaacatta cacaaataag accaataata gtaccaataa ttaatattgc aataccacca
     1801 ataacacaat aaaattgaac cataacacaa cacgctgata ttttaatcaa aaaaagttgt
     1861 atgttattat attctctacg cctacccatt aatattcacc tcactttttc ttaattatag
     1921 tgtatatcat aacaagttat aaataaactt cccgtcacgc gtgacgaagc agttaaattc
     1981 taataagtta tgaaattaac tataataaaa taatattcta tatgcaaaaa aaaaactcct
     2041 gtttattaaa ttatactctc taaaaggggt accgggatca tttcgccttt ttttgacgtt
     2101 aagcaaattt tctgtgcaaa ataaacattt ttcttactat tagatttcag ttcaaatgaa
     2161 tgttacttgt gtaacattaa cttttcaaat ttttcaaaaa agtctttaaa aaataatgaa
     2221 ataaagtggc catataaaaa ccttatattg taaggaattc agacattaac gccaattttc
     2281 ggcgttttga tcccagtacc tctgttaaga ccgaaatacc aaaacaggaa attttaattc
     2341 atctgtagaa tgtttttttt aattagataa aagaaaggaa tggtatttat ggtaaaactt
     2401 acctgtaatg cttgcggtgg taaacttgaa ataacggacg atattgaacg ttttgcctgt
     2461 ggacattgtg gtaatgaatg gatagtgaac agatccgggg gaatagtcag cttaaaggct
     2521 atagaagagc agttatctaa agtacaggaa catacaaagg aaactgctga acattcaaaa
     2581 atattagctg accaagttag attaaaagct attaaggaaa aaataacaac tttatcaata
     2641 gaaacaaatg gaatagataa aaatccacag aaaaagaata atattaaata tgctaaccct
     2701 ttaaaaattg ggtggatagc tttattagtt tttattatat ctttatgtat aagattatta
     2761 ctagacaaat taaaaatcaa aaatattttt gtggattata ttttaggttt acctactatg
     2821 ttatctctta gtattatatg tctttgtatt gttggagcta ttatcaatag tatatattca
     2881 tccttgggaa ataatacctg ttctgatgaa cagcagaaac agattaatat taataaaata
     2941 aaagaactta aaaacgaaat aaataattta gaaaataaag caaaggaaat agaagcagga
     3001 ttttttaagt agaatatact tgtgtaacac cacattcgtg gactcttgct tataataccg
     3061 aaacgtcccg ctattacaga atttaatttt tcaactttac tctttttcca gtggatgagt
     3121 cctcccatac tccagcacat catacttctg agcagtataa agataaacca ttgtagtaag
     3181 gatatactca tgccccagga acctctgcaa tgtcggaagc ctcatggaat tctcataaca
     3241 atgagtagca taagcatgcc tcatgctatg agtgctaaaa acccgcccca tagcctcata
     3301 ctttgccgat atccctgtca tatccccggc accttccacc acccttcgga tctgcctcaa
     3361 agtaattcca aaaacagatt cctccagccc tttcccttcc tgccatgctt taagctgctc
     3421 cagagtccct ttatcaatcg cacaataacg atccttatca cccttgccgg cccttacaaa
     3481 cacagtctta ttatcataaa aaatatcaca gaatttaaga ttttccagtt cctcagccct
     3541 cagtcctgta gcataaagcg tccttattat gaggttattc ctgacagcca gtttcccctt
     3601 cttataagca gttataagct cctggcactc ctcccttgaa agagtagcag gcaatttcct
     3661 gtcacgggtc cagctccaat ctgcaggaag tttcccgaac tcactcttaa gctctgcttt
     3721 aatctccgca atagcacttt tatatttacc agtctgtaac tgttcccctt ctcccggctg
     3781 ctccggctgt tcaccctgcc ccggctcctc tttaactctt ttcatctatt tcacctcagt
     3841 ttctgactaa aaattctcaa aaacccccct tttaggagta attctgtcaa aaatcaccca
     3901 tccaaaaacc tcaaaaaacg tccattttac gccattttag ccctaaaacg ccctgtttta
     3961 cccctaaaat gaccgactta ccgggaatac gtcctcaaat tgacgaagga aaaagtcgga
     4021 catgcttttt gcaagcaatt tttttacaaa attttcataa cattccttac ggccaattct
     4081 ccac
//
LOCUS       DAOQWT010000074         4070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_22527_length_4070_cov_2.923537, whole genome shotgun sequence.
ACCESSION   DAOQWT010000074 DAOQWT010000000
VERSION     DAOQWT010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22527_length_4070_cov_2.923537"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKJ95_02300"
     CDS             complement(<1..697)
                     /locus_tag="PKJ95_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNR65106.1"
                     /translation="MNLWQIVLILVTGILAGLISTLGGGGSLLTMPMLIFLGLPAAEA
                     NGTNRIGLFIQDLVGIVSFRSKGYFYPKISFILGLPAVLGSLLGVRVAISISGELFEK
                     ILGVIMIMVLITILTRPEKRFIKEIEGENWGIIRFAVAILVFFGVGFYGGFIQAGVGF
                     IIIIALTLITGIPLVKVNCLKIFIRFAFTISSLLMFSISGKVNLLLGLTLAAGCSIGA
                     YFGSVIAISKGEKW"
     gene            complement(846..2159)
                     /locus_tag="PKJ95_02305"
     CDS             complement(846..2159)
                     /locus_tag="PKJ95_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNR65107.1"
                     /translation="MQEYLEKKFELSKNGTDLKTEVIAGFTTFMTMAYIIFVNPSILS
                     DAGMPWNGVFIATIMGAILGTVCMALFTNYPFALASGMGLNAYFAYSVVIGTGVSWQI
                     ALGIVFVEGIIFIILSLLPVREMIVNSIPMGLKASISAGIGLFIAFIGLQNAGLVVKD
                     EATLVTIGNIFNGTGLVAISGLIIMGVLHTLKVKGSILWGILAATVIGYIPGLNVTPH
                     FQGLIALPQFSDWSEVLFKMDIKGALNASLIAVIFSFLFVDIFDTAGTLVGVSTQAGY
                     LDKDGNLPRAGGALLADAIGTAGGAIFGTSTVTTYVESASGVSEGGRTGLTGIVVSIL
                     FFLSLFLRPLVGIVPGAATAPALIVVGTMMLSNIFHIKWDDFSEALPAFVCLIAIPMT
                     YSIANGIALGFITYPLIKLLSGRGKEVHGLVYILGILFVLKFIWL"
     gene            complement(2378..3208)
                     /locus_tag="PKJ95_02310"
     CDS             complement(2378..3208)
                     /locus_tag="PKJ95_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR65108.1"
                     /translation="MEIRATVLCENCVFFSSGAIAEHGWSVYLETEQGNFLFDTGGGK
                     AIINNAQCFHKDLSKVKGIILSHSHYDHTGGLFSVLDYTRKINVYSHPEIFKNSYSVR
                     NGKERYIGIPYRREVLESKGADFIFNTNFTEIAPGIMLSGEVPRKTSFEKGDTDLVLK
                     NEKGYVQDIIIDDQTLIINSKKGLVIVLGCSHSGIVNIINYAIEKTGKKHIHAIFGGT
                     HLGPVSEETKNKSIEALKKYDIESLGTSHCTGLEATMRIFREFRERFFFCNVGTVIEV
                     "
     gene            3457..>4070
                     /locus_tag="PKJ95_02315"
     CDS             3457..>4070
                     /locus_tag="PKJ95_02315"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNR65109.1"
                     /translation="MTKSAQRIIIIGNGEDWQKEKVISFCQTADFIIAADNGLSLLHQ
                     LNIVPDLIIGDLDSVPSALLQCYPDTPIEKFPIKKDFSDTELSIKKAITMNPEEIVLL
                     AVIGNYFDHSYATIVNLFRNYKPEIEMKIITSNSLIFPIKKKTTLLHLKGRRFSLFPI
                     SNIKNFTMQGAQYQFPKRNLKVTDYSLSNIIVEEKLEIDLEKGML"
BASE COUNT         1380 a          881 c          614 g         1195 t
ORIGIN      
        1 tccacttttc tcctttacta atggcaataa cactcccaaa ataagcacca atagaacatc
       61 cggcagccag ggttaatcct aataatagat ttaccttacc actaatactg aacataagaa
      121 gtgaagaaat agtaaaggca aatcttataa atatctttag acaattcacc tttaccaaag
      181 gaataccggt aataagggtt aaggctataa taattataaa accgacacca gcctggataa
      241 aaccaccata aaaaccaact ccaaaaaaga ctaatatggc aacagcgaag cgaataattc
      301 cccagttttc accttcaatt tctttaataa acctcttttc cggccgggtt aatatagtaa
      361 tcaagaccat aatcattatt acccctaaga ttttttcaaa caactccccc gagatggaaa
      421 tcgctactct gactcccaga agtgaaccta aaacagccgg taaacccaga ataaaactta
      481 tcttaggata aaaatatcct ttactgcgaa aactaacaat tcccaccagg tcctgaatga
      541 ataaaccaat acgatttgtc ccattagcct ctgctgcagg taatcccaga aaaatcaaca
      601 taggcatagt tagcagtgac ccccctcccc cgagggtact gatcaatcct gccaatatcc
      661 cggtgactaa aattaaaaca atctgccata gattcataat attgtcctca gataattact
      721 gccatcaatt ttaaataaaa atgagttgga ataaagtaaa gctttaagaa taaggttact
      781 ctattccaac ttccaatttc ctgaacactt atattatcag tttactgata tagattcaaa
      841 ctattttata accagatgaa tttgagcaca aatagtatac ccagaatata gactaaacca
      901 tgcacctctt tccctcttcc gcttaacagc ttaatcaagg gataggtaat aaagccaagg
      961 gcaataccat tggcaataga ataagtcata gggatggcaa ttaagcatac aaatgcgggt
     1021 aaggcttctg aaaagtcatc ccatttaata tgaaagatat tgctgagcat catggttccc
     1081 accacaatca aggctggtgc agtagcggct cctggaacaa ttcctaccag aggtctaagg
     1141 aaaagggata agaaaaacag gattgaaacc acaataccgg ttaacccggt ccttccaccc
     1201 tcagaaacac cggaagctga ttcaacatat gttgttacgg tactggtgcc aaaaattgcc
     1261 cctcccgcag ttcctatggc atcagcgagt aaagcaccgc ctgctcttgg taaattgcca
     1321 tctttatcca gataccctgc ttgagtactg accccaacca aagttcccgc agtatcaaaa
     1381 atatccacaa ataagaatga gaaaataacc gctatcaaag aggcatttaa tgctcccttg
     1441 atatccattt taaacagcac ttcactccag tcactaaact gaggcagagc gattaatccc
     1501 tgaaagtgtg gagtgacatt taaaccagga atataaccta tcacagtagc agccaaaatc
     1561 ccccacagga ttgaaccttt taccttcaga gtatgtaaaa cccccataat aatcaatccc
     1621 gaaattgcaa ccaatcctgt gccgttgaat atatttccaa tggttaccag agttgcctca
     1681 tcttttacca ccagtccggc attttgcaaa ccgataaagg caataaataa tcctatgcca
     1741 gcgctgattg atgcctttag ccccattggt atactgttta caatcatctc tcttactgga
     1801 agcaaactaa gaataataaa aataattcct tccacaaaga ctattcccag ggcaatctgc
     1861 cagcttactc ccgtaccaat taccactgaa taggcaaaat aagcatttaa tcccattcca
     1921 gatgctaagg caaatgggta attagtaaat aaagccatac atactgttcc cagaatggca
     1981 cccattatgg tggcaataaa aacaccattc cagggcattc cggcatcact taaaatagag
     2041 ggattgacga aaataatgta agccatggtc ataaaagttg taaaaccagc aataacctct
     2101 gttttcagat cggttccatt tttggaaagt tcgaattttt tctccaaata ttcctgcatt
     2161 tttgctcctt ttctaatatc ttttataaaa tcttttatta aaatttaaat attcttcata
     2221 ttcaacattc ttttcctatc ttattatttt taccaggatt tatcaatact ttatgataat
     2281 ttttacctgt taaaatattt tttttaaagc ctattttctc caggattggc aactcttatt
     2341 tgaaatataa tttttttatt tttcaccttt tatattatta cacttcaatt actgtaccta
     2401 cattacagaa aaagaagcgt tctctaaatt cccggaatat gcgcatagtt gcttccaatc
     2461 cagtacaatg ggaagtgcct aaactttcaa tatcatactt ttttaaggct tctatactct
     2521 tgtttttagt ctcctcactg accggaccaa gatgagttcc tccaaaaatg gcatgaatat
     2581 gcttcttacc ggttttttca atagcataat ttataatatt gacaatgcct gaatgtgaac
     2641 aacccagcac aattaccaat ccttttttgg aattaattat taaagtctgg tcatcaataa
     2701 taatatcctg cacataccct ttttcatttt ttagtaccag atcagtatca cctttttcaa
     2761 aagaagtctt gcggggcact tctccgctta acattatacc cggggcaatc tccgtaaagt
     2821 tggtattgaa gataaaatct gcccccttac tttccagtac ttctcttcta tagggaatcc
     2881 cgatatagcg ttcttttcca ttgcgtacac tgtagctatt cttaaaaatt tcaggatggg
     2941 aatagacatt aattttcctg gtataatcaa gtactgaaaa aagccctccg gtatggtcat
     3001 agtgactatg gcttagaata atacccttaa ccttggaaag gtctttatgg aaacattgag
     3061 cattattaat aattgccttg ccaccaccgg tatcaaaaag aaaattgccc tgttctgttt
     3121 ccagataaac cgaccagcca tgttcagcaa tagcaccact actaaaaaat acacaatttt
     3181 cacaaagaac tgttgctcta atctccataa tcccctccag agttaatctc agatatgttc
     3241 cagcattgca ttctcatcta tataaaagga tactacttat cccagttctg gtaaagggta
     3301 ttttgtaata gtaaagatta ttttctgctg gattatctta aaaaatggaa ggcctctact
     3361 cttttatata ttatggctga gacagctaca ttaatttgaa aagtgtgtcc agatgtaata
     3421 atataagaaa caaaatatac aatgggttaa tacactatga ccaaatcggc acaaagaatt
     3481 atcataatag gaaatggaga agactggcaa aaggagaagg taatatcctt ttgccagaca
     3541 gctgatttta ttattgcggc agataatgga ttatctttgc ttcatcagct taatatagtt
     3601 ccagacctca ttattggcga tctggatagt gtaccatctg ctttattgca gtgctaccct
     3661 gatacaccta ttgaaaaatt tcccatcaaa aaagatttca gtgatacaga gctaagcatt
     3721 aaaaaagcta ttactatgaa tcctgaagag atagtacttc tggcagtaat cggtaactac
     3781 ttcgaccata gttatgccac cattgtcaat cttttccgaa attataagcc agaaatagaa
     3841 atgaaaatta ttacctcaaa ttccctcatt tttcccatta agaaaaaaac taccttactc
     3901 cacctgaaag gaagaagatt ctctctcttc cccatcagta acataaaaaa tttcactatg
     3961 cagggggctc aatatcaatt tcccaaaagg aatttaaaag tcaccgatta cagtctcagc
     4021 aacataattg ttgaggaaaa actggagatt gacctggaaa aaggaatgct
//
LOCUS       DAOQWT010000075         4021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_22888_length_4021_cov_3.563036, whole genome shotgun sequence.
ACCESSION   DAOQWT010000075 DAOQWT010000000
VERSION     DAOQWT010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22888_length_4021_cov_3.563036"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PKJ95_02320"
     CDS             <1..693
                     /locus_tag="PKJ95_02320"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNR65110.1"
                     /translation="IFIIILILLFFFAGCSKPKKPKLSLSEDSWYYGEVKPDQVVSHQ
                     FTLKNEGEGKLTIDSFYSSCACVILEMDKKEIAPGGEGTLKAIFDPYGYEGFVTKEVT
                     INSNDPEHPKKIIDLSITVLRVPHPDIKLSQQTFDLGTITISGLSVLEFTISNEGDAD
                     LIIEDIVTEEIFSHNLVLPLILLPGEQYLSEVYIDTSQIKEEVFRKAIRIMTNDPQNS
                     MVFLRITGTTKK"
     gene            complement(929..2080)
                     /locus_tag="PKJ95_02325"
     CDS             complement(929..2080)
                     /locus_tag="PKJ95_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65111.1"
                     /translation="MEVREVKKNKSELLQFINFACRVYEHDPHWVSPIKTDLLKSFLS
                     EGSDNKINCGPHTFFMVWDNNIPVGRVLVGINVEKNHRNNINVGYFGYLEMINSYEVL
                     KMLMDESFLWLKKHNINRLVGPLCPDDDVEGRGILIKGFDSPPVLMNSYNPVYYHELL
                     SQYGFTKDRDFYAYYSDNFNELKGRVEKVAEFAMQKFNFKIDKVDLKYLEREIKDIAE
                     IVEKIISSGDETENGFEYANPPNKDSLLIEVKKYLPFLDKDLIYIARSENRPIGFVFA
                     IPDYNYVLKKINGKLDPLGLIKFLWYKNKIKGIRGFAQFVVPEFQNKAVNAAIFKHIL
                     EVAERKQYNYIEGSCICDNNLPSRRIFENAGLSPYKVYRVYCKDSNIVS"
     gene            2425..3234
                     /locus_tag="PKJ95_02330"
     CDS             2425..3234
                     /locus_tag="PKJ95_02330"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNR65112.1"
                     /translation="METFKNNELELKINLEPNSRLINKLMESEEKYKILFDYAPDGYY
                     ISDLKGTFIDANRAAERISGYTCDELLGKNYFQAGLLSIKDVPKALRALAKNKYTAGH
                     QLRVTKLAVAIAQEMNLSPDHVESVKIASQIHDIGKIGIPSEILTKPTNLDSIEFSFI
                     KEHPRMGYNILKDVDFSYPIADIILQHHERNDGSGYPQGITGESIILEAKIIAVADVV
                     EAMTSHRPYRAAMGIDAAMDEILKNKGTLYQPEIVDVCIKLFKEKGFNFLI"
     gene            complement(3168..>4021)
                     /locus_tag="PKJ95_02335"
     CDS             complement(3168..>4021)
                     /locus_tag="PKJ95_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR65113.1"
                     /translation="IPQTFYPPGQLYEQVQERVKRSREREEHIDYLTFVPDGEPTLDI
                     NLGEEIKLVKSLGIKVAVISNGSLLDQRKVMENLLDVDLVSLKVDSVDEDIWKKINRP
                     HKKLNLNTILNNMLKFVSVFKGKIITETMLVKGVNDGIEHMERVAEFIAQLNTEKVYL
                     SIPIRPPARKWVQAPQEEVINRCYNIFKKSISQVEYLIGYEGNAFAFTGEVAKDILSI
                     TSVHPMREEAMRDFLKKAGSDWTVIHKLIEEGRLVETEYEGKKFYIRKLKPFSLNNFI
                     HTSTISG"
BASE COUNT         1318 a          738 c          614 g         1351 t
ORIGIN      
        1 atctttatta ttattctgat attactgttt ttttttgctg gatgcagtaa accaaaaaag
       61 cccaaacttt ctctttcaga agatagctgg tattatgggg aagttaagcc tgatcaggtt
      121 gtttcacatc aatttacttt aaaaaacgaa ggtgagggca aactcaccat tgattcattt
      181 tactcttcct gtgcctgtgt tattctggaa atggataaaa aggagatagc acccggggga
      241 gaaggaacgc ttaaagctat ttttgatcct tatggctacg aaggatttgt aaccaaagaa
      301 gttactatca attccaatga ccctgaacat cccaaaaaaa taatcgattt atctataacg
      361 gtattaagag tcccccatcc tgatataaaa ctttcacagc aaacctttga cctgggtacg
      421 attaccatat cggggctatc tgtactggaa tttactatca gcaacgaagg ggatgccgat
      481 ttaataattg aggatatagt tacagaagaa atattcagtc ataacctggt tttacccctt
      541 atactacttc ccggagaaca atatctgtct gaagtctaca tagataccag ccagattaaa
      601 gaagaggtat tccgcaaggc aatccgcatt atgaccaatg accctcaaaa ttccatggta
      661 ttcctacgta tcactggcac taccaagaaa tagaatggta tttgataaat aataaagtga
      721 atctaatcta ccagaccctg gtagatatat actccctaca gtcagcaacc agacattaaa
      781 agagttaata ataaaaaaac agtgagatat attaattttc ttaacattgc tatcctccct
      841 atttctttta tttataatta tttttatctt tagaaaatct tattctaaat taaaagagaa
      901 ctaaaccagt tatcttttac tatcattttc aagaaacgat attactatct ttgcaataaa
      961 cccgatatac cttatatggg gaaagtccgg cattctcaaa tattcgtcga gagggtagat
     1021 tgttatcaca aatacaagaa ccctcaatat agttatattg ttttctctct gctacctcta
     1081 aaatgtgctt aaaaattgcc gcattaactg ctttgttttg aaattcagga accacaaatt
     1141 gtgcaaaacc tcttatccct tttatcttgt ttttatacca gagaaatttt atcaaaccca
     1201 gcggatcgag tttaccatta atctttttca atacataatt atagtcaggt atagcaaaaa
     1261 caaagccaat gggtctattt tctgagcgag caatataaat cagatcttta tctaaaaagg
     1321 gcaagtattt tttaacttct atcaataggg aatctttatt aggggggttg gcatattcga
     1381 atccattttc tgtttcatct cctgatgaga ttattttttc tactatttca gcaatatctt
     1441 ttatttcgcg ttctagatat ttcaaatcta ctttgtcaat tttaaaatta aatttttgca
     1501 ttgcaaattc tgcaaccttt tcaactctac ccttcaattc attgaaattg tcagaatagt
     1561 aggcataaaa atccctatct ttagtaaatc cgtattgact taacaattca tggtaataaa
     1621 ccggattata tgaattcatc aataccggtg gactatcaaa acccttaata agtatacccc
     1681 tgccctcaac atcatcatcg ggacaaaggg ggcctaccaa acgattaata ttatgttttt
     1741 tgagccagag gaatgattca tccataagca tttttagaac ttcataagaa tttatcattt
     1801 ctaaataacc aaaatagcca acattaatat tatttctatg attcttctct acatttatac
     1861 caactaaaac cctacctact ggaatattat tatcccaaac cataaaaaag gtatgtggcc
     1921 cacagtttat cttattatca gaaccttcac ttaaaaagga ttttagcaaa tctgttttta
     1981 ttggagaaac ccaatggggg tcatgttcgt atactctaca ggcaaaatta ataaattgta
     2041 aaagttcgga tttatttttt tttacttctc ttacttccaa ctttgttctt ctctttctcc
     2101 aaaaatagca acgcttttct ttataatgga tttttgcttt ttaaattgtt gtagtgttgg
     2161 tgaatttttt tcatacatta tcttaaatat tttagcattt taataattct attcaataaa
     2221 agacattata ttgtaatttt gaatatttat caaaattaat ttttattaga gctacggtat
     2281 gtgttttaca atcagtatac tagtaattaa ccggtattgc aaagtcgtta tataataata
     2341 taagaaataa tttatgcaat ttttttctgg tattcttatt aagtgatata atattttaga
     2401 ttttactgta cagagaggca taccttggaa acgtttaaaa ataatgaatt ggagctaaaa
     2461 ataaatctgg aaccaaattc acgtttaata aacaaattaa tggaatctga agaaaaatat
     2521 aaaattttgt tcgattatgc ccctgacgga tattatatca gtgaccttaa aggaaccttt
     2581 atcgatgcaa atagggcagc agaaaggatt agcggatata cctgtgatga actattaggt
     2641 aaaaactatt tccaggccgg attgttgtct ataaaagatg tgccaaaagc attgagagct
     2701 ttagctaaaa acaaatatac cgctgggcat cagcttagag ttaccaagct tgctgtcgca
     2761 attgctcaag agatgaatct ttcgccagac cacgttgaaa gcgtaaagat agcatctcaa
     2821 attcatgata ttggtaaaat tgggatacct tcagaaatcc ttaccaagcc aactaattta
     2881 gattcaatag agttctcttt tataaaagaa catcccagaa tgggttataa cattttaaaa
     2941 gatgtcgact tctcctaccc cattgccgat attatacttc aacaccatga aagaaatgat
     3001 ggctccggtt atccgcaggg gataacagga gaaagtatca ttttagaagc aaaaataatc
     3061 gcagtagcag atgtggtaga agctatgacc tcacaccggc cctatagggc agcaatggga
     3121 attgatgcag caatggatga aatattaaaa aacaaaggta ctctttatca gcctgaaata
     3181 gtagatgtgt gtataaaatt attcaaagag aagggtttta attttctgat atagaatttc
     3241 tttccttcat attcagtttc cactaatcgg ccttcctcga ttaatttatg aataacagtc
     3301 caatcagaac ctgccttttt taaaaaatct ctcatcgcct cttctctcat gggatgaaca
     3361 gaggtgatac ttaaaatatc tttcgctacc tcgccggtaa aagcaaaggc atttccctca
     3421 taaccgataa ggtattcaac ctggctaata ctttttttaa agatattata gcatcggtta
     3481 ataacctctt cttgaggtgc ttgaacccat ttcctggcgg gaggcctgat gggaattgac
     3541 aaataaacct tttctgtatt tagctgtgct ataaattctg ccactctctc catatgttca
     3601 ataccatcgt taacgccttt caccagcatt gtttctgtaa ttattttccc tttaaaaacg
     3661 gaaacaaatt ttaacatatt atttaaaatt gtatttaaat taagtttttt atgaggtcga
     3721 ttaatttttt tccatatatc ctcatccaca gaatcaactt ttaaagaaac caggtctaca
     3781 tccagtaaat tttccatcac ttttctctgg tccagtagcg agccgttact aataaccgcc
     3841 actttaatgc ccagggattt tactaattta atttcttctc ccagattaat atccagggta
     3901 ggctctccat ccggtacaaa agtcagataa tctatgtgct cttccctttc tctgctcctc
     3961 tttactcttt cctgaacctg ttcataaagt tgcccggggg gataaaaagt ttgtggaatt
     4021 a
//
LOCUS       DAOQWT010000076         3992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_23115_length_3992_cov_2.454915, whole genome shotgun sequence.
ACCESSION   DAOQWT010000076 DAOQWT010000000
VERSION     DAOQWT010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23115_length_3992_cov_2.454915"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKJ95_02340"
     CDS             complement(<1..439)
                     /locus_tag="PKJ95_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65114.1"
                     /translation="MPEMLNDSRRKFLGYFEPAKRSTVVNIFLSQIRKGLTDPDIIIT
                     EVYEKARKDRDKHYISDTIFKYLSDAFKYVSYLFDWEHLPDSEKQYYKNIQAKKAVKA
                     YMAKEKPTDKQLSYLKALGYTGPAPDSKADACELISTIKVFNKI"
     gene            complement(432..1142)
                     /locus_tag="PKJ95_02345"
     CDS             complement(432..1142)
                     /locus_tag="PKJ95_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65115.1"
                     /translation="MSNKAHLMTGYSKNRRPYDFYSTPPARVMALLENETFSGSIFLE
                     PCSGAGHISKVLEKKFPGQNILSYDIRKDNIYGTGGIDFLKQTAEFYNHPDHIITNPP
                     YKILTEFIQHSKTIARYKIAMLLPTQALGGKIHNGLIWTDKQFPLKKVIIIPDRLKFF
                     STKNSCPMYHLWAIWDRFHVGEPIITWHQKKENTLPLFPEGEEEQEEDENFKDAMETE
                     VLLLQKQIEIESEVFCDA"
     gene            complement(1869..2039)
                     /locus_tag="PKJ95_02350"
     CDS             complement(1869..2039)
                     /locus_tag="PKJ95_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65116.1"
                     /translation="MKIKENKSLRYVKISDKHHAELKRKAVDCRQSLQDYVNMVLENF
                     LKNNTTQIIQTG"
     gene            complement(2191..3126)
                     /locus_tag="PKJ95_02355"
     CDS             complement(2191..3126)
                     /locus_tag="PKJ95_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65117.1"
                     /translation="MLLKAKTDKSKGFKFKLADMELTKIINQLPLYNLNDSVIYDLCE
                     LLVYDYFGDFFVVTEENAKIFNIAMEENPPVVDSISFLTGVMNIYPFLPSLFHISLSI
                     PVGAIQILLAIDKNDIVRPVKLRLNEEVKGELIAMCHLSINNMSMKLKEIETKIFALP
                     KDDKTQNHITIFPNYSDKQEKKPLVFWHLAFKDKTETSIDEIILLTSAWDTEEDFLKR
                     AKNKGVSISNRTLYQYRTFSLFPDPYKTTVKGKETNFYDPKWIKYIKRVQELKSKLNK
                     TNLPLKDIRKLDEKFFKDIPLKKKKNNKSKKNKTV"
     gene            complement(3140..3907)
                     /locus_tag="PKJ95_02360"
     CDS             complement(3140..3907)
                     /locus_tag="PKJ95_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNR65118.1"
                     /translation="MKRKTKKKPKISKIKIINSFKKAYRNLIAVRTFEQYKIVLKQIL
                     SGKIKITSKSKYRQYKAVINKVKNIGIELPGKELPKWSKKGDATKKNIIDKYISPETL
                     EIILANVPRGENGRELARAIQLSYYSGLRLSEVINLKRKDIVIDGIIKFNIQGKGDKY
                     REVFLPRDRKDLLEGFHGFTITEGYVKNAITRIRERIGVNFSFHSFRHTFATNFLKER
                     GGNIKLLQELLGHESLATTSLYLRIVGKAEEMAKLGF"
BASE COUNT         1112 a          760 c          566 g         1554 t
ORIGIN      
        1 atattttatt aaaaactttt atcgttgaaa ttaattcaca ggcatctgct ttactgtcag
       61 gtgccgggcc tgtgtaacct aaagccttaa gatatgatag ttgtttgtct gtaggctttt
      121 cttttgccat ataagcttta actgcttttt tggcctgaat atttttgtaa tactgctttt
      181 ctgagtctgg caagtgctcc cagtcaaaaa gataactcac atatttaaaa gcgtctgaca
      241 ggtatttaaa aatagtgtct gaaatataat gtttatctct gtcttttctg gctttttcat
      301 atacttcagt tatgataatg tccgggtctg tcaggccttt tctaatttgg cttaaaaaga
      361 tatttaccac tgtcgaccgc tttgcaggct caaaatatcc aaggaatttc cgacgtgaat
      421 catttaacat ctcaggcatc acaaaatacc tcactttcta tttcaatctg tttctgtaac
      481 aataaaactt ccgtctccat agcatcttta aaattttcat cttcttcctg ttcttcctca
      541 ccttccggga atagtggtaa tgtattttcc tttttttggt gccatgttat gatgggctcc
      601 ccgacatgaa aacgatccca tatagcccat agatgataca ttgggcaaga atttttagta
      661 gaaaaaaatt tcagtctatc aggtattatg attacttttt tcaaagggaa ttgcttgtca
      721 gtccatatta aaccgttatg aatttttcca ccaagggcct gagtcggtaa taacattgca
      781 attttatatc tggctattgt tttgctatgc tggataaatt ctgtgagtat tttatatggt
      841 gggttcgtga taatgtgatc tggatggttg tagaattctg cagtctgttt tagaaagtcg
      901 atcccaccgg tgccataaat attatctttc ctgatatcgt atgaaagaat gttctgtcca
      961 gggaatttct tttctaaaac tttagatatg tgaccggcac cgctacaggg ctccaaaaaa
     1021 atacttcctg aaaaagtctc gttttccaat aaagccatta ctctggcagg tggggtcgaa
     1081 taaaagtcat agggtcgtct atttttgcta taccctgtca ttagatgggc tttattgctc
     1141 actgttgcta tcctcctgaa aatttccggt atgaaggata taatgtccgt caacctcctg
     1201 aagttgatct gagtcaacta cgtcaaataa attccggttg aacttacaac attcacagaa
     1261 aaggatcccc cgggtacagt ttaaccggcc ctgattgaac tcaaatgtca tgagcctgcc
     1321 acatttcgga caaacttttg atgttaccgg tcttatgctt ctatattcat taattactgg
     1381 catctttaat aatcgctcct ttttaataat aataaagttt gcttatacca cttatgtata
     1441 ctcttagata taagtaaaaa tttgttattg attaagcttt atcaatttct ctttaatctg
     1501 tttctctatg accgccggtc ttttacttta ccgccatccg ccctagcttc taccggctaa
     1561 ctgtcacctc gccgtttgtc atcccctgct tagctctgcc tcctgccact ccagccctcc
     1621 tcttgtctcc actccagaca gaccactaca ggctttacac taccgcccac cattcaattt
     1681 cctttggtat ttttggcaaa tttcaactaa tttctgactt gttttttcta ttaaacctga
     1741 tataataagc ttaacggaaa gacttttgat aataaaagtc tctttacatt tcttacggaa
     1801 tgtacttttt aaagtttcta tataggtatc tatcgatatt aacggtatcg aaaggtacct
     1861 ttaatatttt atcccgtctg tattatctga gtagtattat tctttaagaa attttccaag
     1921 accatattta cataatcctg taaagactgc cggcaatcga ccgcttttcg ttttagctct
     1981 gcatggtgtt tatctgaaat ttttacatac ctgagacttt tgttttcctt gattttcatt
     2041 tgctttcctc cttcctataa gtattttact aatattaaaa gttaacttat tgtttttttt
     2101 attatacaac agatataaaa ttcaagtcaa tataaacttt tttcatatta cctctgaagg
     2161 gtatactctt catactatct ctgaaaatgc ttatactgtc ttattctttt tgcttttatt
     2221 atttttcttc ttttttaaag gaatatcttt aaaaaatttt tcatccaatt ttctaatatc
     2281 ttttaatggc aaatttgttt tatttaactt gcttttcagc tcctgaactc tttttatata
     2341 tttaatccat tttggatcat aaaaatttgt ttcctttcct ttaactgttg ttttataggg
     2401 atctggaaat aaagaaaaag tcctatattg gtataaagtt ctattactta tagagacacc
     2461 tttatttttt gctcttttta aaaaatcttc ttctgtatcc catgctgaag ttaataatat
     2521 tatttcgtca atgcttgttt ctgtcttatc tttaaaagct aaatgccaga aaacaagagg
     2581 ttttttttct tgcttatcac tataattagg aaatatagtt atgtggtttt gtgttttatc
     2641 atcttttggc aaggcaaata tttttgtttc tatttctttt aacttcatac tcatattatt
     2701 tatagataaa tggcacattg ctattaattc accttttacc tcttcattta atcttaattt
     2761 tactggtctt actatatcat ttttatctat ggctaataat atttgaatag ctcctacagg
     2821 tatgcttaaa gatatatgaa ataatgatgg taaaaaagga taaatattca taactccagt
     2881 caaaaaacta atagaatcca ctacaggagg attttcttcc atagctatat taaatatttt
     2941 tgcattttct tctgttacaa caaaaaaatc cccaaaataa tcatacacaa gcaattcaca
     3001 aagatcataa ataactgaat cgtttaaatt atataaaggt aactgattaa ttattttagt
     3061 aagctccata tcagctaatt taaatttaaa tccttttgat ttatctgttt tagcttttaa
     3121 taacattttt tctctctcct taaaacccaa gtttcgccat ctcttcagct ttccccacaa
     3181 tacgaagata cagggaagtt gtggcaaggc tttcatgtcc taataattcc tgtaacagtt
     3241 taatattccc acctctttcc ttcagaaaat ttgttgcaaa tgtatggcgg aaggaatgga
     3301 aagaaaaatt aacgcctatc ctctctctaa ttctggtaat ggcatttttg acataaccct
     3361 ctgtaattgt aaacccatgg aatccttcca gcagatcttt tctgtccctg gggagaaaga
     3421 cctctctgta tttatccccc tttccctgta tgttgaattt aataatgccg tctataacaa
     3481 tatccttcct tttcaaattg attacctctg acagcctgag ccctgagtaa taacttagct
     3541 ggatggctct tgcaagttcc cggccattct cgcccctggg cacatttgca agtataattt
     3601 ccagtgtttc cggtgatata tacttatcaa taatattctt ttttgtagca tcaccctttt
     3661 tactccattt cggtaattct tttccgggta attctatacc aatatttttc actttattta
     3721 tgactgcctt atattgacgg tacttagatt ttgacgttat ttttatctta ccggatagta
     3781 tctgttttaa cactatttta tactgctcaa aagtccttac tgctattagg tttctatatg
     3841 cttttttgaa ggaatttatt atctttatct tgcttatttt aggttttttc ttagtctttc
     3901 ttttcaagtt atcacctgct tttgttaaat atttgattac ataacaagga ttatgtaatc
     3961 attatagcag aattacataa tactatcaat ga
//
LOCUS       DAOQWT010000077         3981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_23193_length_3981_cov_3.921803, whole genome shotgun sequence.
ACCESSION   DAOQWT010000077 DAOQWT010000000
VERSION     DAOQWT010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23193_length_3981_cov_3.921803"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1445)
                     /locus_tag="PKJ95_02365"
     CDS             complement(93..1445)
                     /locus_tag="PKJ95_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65119.1"
                     /translation="MLVLLINPIVLISFCVFLGILFGNIKLGRFSFSTSGSLFVGIVA
                     GWLIVKYIHTIGPESEFFGIAQNILSKDIVDKKFFDLFLVLFIASVGLLAARDVGRVI
                     KKYGLKFITLGFLITFIGAATTYGFSLFYPVRNPYLYTGVYTGALTSSPGLGAALESV
                     RVHSAELADRFSELNEPERKRILNIVGYEGYYDTNSSFPLSQEEKDKLSKNSEAAVGT
                     GYAVSYPFGVIVVIFAMNFFPLIFKIDTDKEKLLLIEELQNHSEKTINKIKEVYFDLS
                     AFSLVCLVGYFLGILKITIPSLGELSLGSTGGVLIAALILGYLGNIGPFCFRMDSKVL
                     GTIRTITLAYFFAVIGLNYGYRVIDSLSGPGLFLAFSSLFIGSIAMIIGFLVGRYLFK
                     INWIMLSGAICGGMTSTPGLGAAIDSIGCDDPAAGYGAVYPFALLGMVVFSILLNHII
                     "
     gene            complement(1539..2864)
                     /locus_tag="PKJ95_02370"
     CDS             complement(1539..2864)
                     /locus_tag="PKJ95_02370"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65120.1"
                     /translation="MTIAFIRHPEIKKENIPDSVLFIIKRLEGAGFLSFLVGGCVRDL
                     ICQKISKDWDIVTDASPGQIKKIFFDYKALLIGRSFQTITLIIDSQTYHISTIRGIGG
                     IGVGENWKNNLLAEQEKYQLIINDLLCRDFTINSLALNPDKGILDPSGGLPDLNRKLI
                     RSLKPTIRFREDPLRMIRAVRIACQLNFTVDCLTGKSIIKHAFLINQVSPERIREELY
                     AVFQTSEAKRGIILLRQYGLEQYIFSIDKIKRERKGAKEWELIPSTGFDELKEELSCW
                     LAVWGRFSFGSCRQARILYIPLLYNLKFHREIIKKLKILFSREWVTMDYSSGTNIRFL
                     MSELGRENCKTMFYLKKMLLWSEKKVDMLAKLKREEELLKNELKKNAPISFKDLAIKG
                     EDLIEMGIPEGKWLGEVLQSLLTEVLVSPENNNKDFLIRMAKKFRANYY"
     gene            3032..3451
                     /locus_tag="PKJ95_02375"
     CDS             3032..3451
                     /locus_tag="PKJ95_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNR65121.1"
                     /translation="MKDIYEQILKLKYFCHCHERRISAYCQINLAELKGLKAMEGEEP
                     ITCSDFSKKINLSPSRSSRIIDNLVRKGLLLRKIGEDDRRSTLLYFTDKGWNLKESIK
                     LEEEKFEQLLTSELGSEDIEIIRKGLKLLEKIMENNF"
     gene            3463..3792
                     /gene="trxA"
                     /locus_tag="PKJ95_02380"
     CDS             3463..3792
                     /gene="trxA"
                     /locus_tag="PKJ95_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015220403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNR65122.1"
                     /translation="MSEKTLIEVNESNFQEEVLDSPLPVLVDMWAPWCMPCRMVAPSV
                     EKIAQDNNAKLKVCKLNVDDNQNIAVRYNIQRIPALLIFKDGKEYDRIVGAVPPQAIQ
                     SKLDKIL"
BASE COUNT         1336 a          748 c          613 g         1284 t
ORIGIN      
        1 gcagaaattc tgtgaatcct ttatcagtaa ggagcctttg gagataaaaa atattgaatt
       61 tgctatttaa ttctatctca ttattgagtc ctttagatga tgtgattcaa caatattgaa
      121 aataccacca tacctaaaag agcaaaagga tatactgctc cataaccggc tgcaggatca
      181 tcacatccta tggaatcaat tgcagcacca aggccaggag tagaggtcat tcccccgcaa
      241 attgcgcctg ataacataat ccagttaatt ttaaagagat atcttcccac taaaaaaccg
      301 ataatcattg ctatggagcc aataaataaa gacgaaaagg ccaggaacag ccctggtcct
      361 gatagagaat caattactct gtaaccataa ttaagaccga taacggcaaa gaaataagcg
      421 agagttattg tccgaatagt acccaaaact ttgctatcca ttctgaagca aaatggccct
      481 atatttccta aatatcctaa tattaaggca gctatcaaaa ccccacctgt cgagcccaga
      541 cttagctctc ctaatgaggg tatggtgatt ttaagaatac ctaaaaaata tcccaccagg
      601 cagaccagag aaaaagcact caggtcaaaa tagacttctt ttatcttatt aatagttttt
      661 tcactatggt tttgcaattc ttcaatcaat aataatttct ccttatctgt atctatcttg
      721 aagataagag ggaaaaaatt catcgcaaaa attactacta ttactccgaa gggataactg
      781 actgcatagc cagttcccac tgcagcctct gaatttttgg acaatttatc tttttcttct
      841 tgggataaag gaaaagaaga gttggtatca taatatcctt catagccaac aatgtttaaa
      901 attctctttc tttcaggctc atttaactct gaaaatctgt cagctaactc agcggagtgt
      961 acacggacag attccagtgc agctcctaat cccggtgagc tggttaaggc accggtatag
     1021 acaccagtat ataaataagg attcctcacc ggataaaaca gactgaatcc ataggttgta
     1081 gccgccccta taaaggtaat tagaaaaccc agtgtaataa atttcaagcc atactttttt
     1141 attactcttc ccacatctcg ggctgcaagc agcccaactg aggcgataaa aagtaccagg
     1201 aataaatcaa aaaatttctt atctacaata tctttactca atatattttg ggcaatgcca
     1261 aaaaactcac tttccggtcc aatagtatga atatatttga ctattaacca ccctgccact
     1321 ataccaacaa acaatgagcc agaagtacta aaactaaatc ttcccagctt tatgttgcca
     1381 aataatattc ccaaaaaaac gcaaaaactt attaaaacaa ttgggtttat taatagtact
     1441 aacatgagat gccttctttc ttaatggttt ttttatatcg gatttatatc acattttgct
     1501 tatgatgacc taccttatgc caaatatatt ttcaattact aataatagtt tgctctaaac
     1561 tttttagcca tccttatcag gaaatcttta ttattatttt ctggtgaaac cagtacctcg
     1621 gtcaacaatg attgtaatac ttcccctaac catttccctt caggtattcc catttctatc
     1681 aaatcttctc ctttaattgc taagtcttta aaagaaatgg gtgcattttt ctttaattca
     1741 ttctttagca actcttcttc tctttttaat tttgccagca tatccacttt tttttctgac
     1801 cagagcaaca ttttttttaa ataaaacata gttttacaat tttctcttcc caattctgac
     1861 attagaaagc gtatattcgt accagaactg taatccattg ttacccattc tctactgaac
     1921 agaattttaa gtttttttat aatttcccta tgaaatttca gattatagag aagaggtata
     1981 tataatattc gggcctgtcg gcaggacccg aaagagaatc taccccaaac agccagccag
     2041 caagatagtt cttcttttaa ctcatcaaac cctgttgaag gaattaattc ccattccttg
     2101 gcacccttcc tctctctttt tattttatct atactaaaaa tgtattgctc caacccatat
     2161 tgcctcagta atattatgcc tctttttgct tcagaagttt gaaaaactgc atacagttct
     2221 tctcttattc gctccggact gacctgatta attaaaaaag catgttttat tatactttta
     2281 ccggttagac aatctacagt aaaatttaac tgacaggcta tacgaacagc tcttatcatc
     2341 cttaaggggt cttctctaaa acgaatggta ggttttaaac tacggatcag ttttctattg
     2401 aggtctggta acccacctga ggggtctaaa atacccttat caggattcaa ggctaaagaa
     2461 ttgatggtga aatcacgaca gagcaaatca tttatgatga gctggtattt ctcttgttca
     2521 gctaataaat tatttttcca attttctcca acacctatac ctccaatacc tctaatggtc
     2581 gaaatatgat aggtttgaga atctatgatc agtgttatag tctgaaaact tcttcctata
     2641 agcagggctt tataatcaaa aaaaatcttt ttaatctgtc cggggctggc atcagtaaca
     2701 atgtcccaat ccttactgat tttctggcag ataagatccc tgacgcaacc ccctaccaaa
     2761 aatgataaaa aacctgctcc ctctaatctc tttattatga acaagacaga atcgggtata
     2821 ttttcttttt taatctcagg atgtctaata aaagctatgg tcatttatct taacaccaac
     2881 atattatttt ctattctcga tctttttcat attcataatt ctattaatat ttttaagata
     2941 atacaaatta ttaccaaatt aagcttgaca aaccattaga ttatgttatt attgctatta
     3001 aatatatttg ttagaagcaa tgattgttat tatgaaagat atctatgaac aaattttgaa
     3061 attaaaatat ttttgccatt gccatgaaag aaggattagc gcttactgtc agattaacct
     3121 ggcagaattg aaaggactca aagcaatgga gggagaagaa cccattactt gttcggactt
     3181 ttctaaaaag attaacctat ccccttctcg tagcagcaga attattgata atctggtcag
     3241 aaaaggttta ttgttacgaa agataggaga agacgaccgc aggtctacac tcctttattt
     3301 caccgacaaa gggtggaatt taaaggagag tataaagctg gaagaagaaa aattcgaaca
     3361 gcttttaaca tcagaactgg gtagtgaaga tattgaaata atcagaaaag gtttaaaatt
     3421 attggaaaaa ataatggaaa ataatttcta aaggagataa ctatgtcaga aaaaacattg
     3481 attgaagtaa atgagagtaa ttttcaggag gaagtgctcg attcccctct tcctgtattg
     3541 gttgatatgt gggcaccctg gtgtatgccc tgtagaatgg ttgcaccgtc agtagaaaag
     3601 attgcccagg ataataatgc taagttaaag gtatgtaaat tgaatgttga tgataatcaa
     3661 aatattgcag tccgttacaa tatacagagg atccctgctc ttttaatatt taaggatggt
     3721 aaagagtatg atcgtattgt gggagcggtt cctcctcaag ctattcaatc aaaactggat
     3781 aaaatacttt agttttcaaa ggttaacaga taataaacag ataataatcg attaatgata
     3841 gggtatatat ttatataatt ttttccatag atatataccc ttttttaaat catacttata
     3901 ttatcaaaac attatgaaaa aattatcaag aatatatgtt gtttacgttt acatggcaaa
     3961 tatcaatcag attttatcat t
//
LOCUS       DAOQWT010000078         3967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_23319_length_3967_cov_3.042178, whole genome shotgun sequence.
ACCESSION   DAOQWT010000078 DAOQWT010000000
VERSION     DAOQWT010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23319_length_3967_cov_3.042178"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PKJ95_02385"
     CDS             complement(<1..580)
                     /locus_tag="PKJ95_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65123.1"
                     /translation="MLETKRLTKRFGGLVAVNQFDLEVLKGKISSIIGPNGAGKTTVF
                     NVITGIYHPDDGEYYFKGERLFGLKPHQILSKGIARTFQNIRLFPNMTCLENVMAGQH
                     ARGNSGMWQSIFRTPAQLNEEKRIMKIAEDRLEQVGLSCYKHELAKNIAYGNQRMLEI
                     ARALASEPGLLILDEPSSGLNDKETEDLMGFLD"
     gene            complement(637..1644)
                     /locus_tag="PKJ95_02390"
     CDS             complement(637..1644)
                     /locus_tag="PKJ95_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR65124.1"
                     /translation="MKLKNENSQVNRLSRKNNWFVILLILLFLLFPLVIKNDYFIDVA
                     FFFGIYTLLGLSLNIVLGEVGLFDLGHMGFAAIGAYTTAILNTRFGIPVLILLPISAI
                     AAAIFAYLVCSPIIHLKGDYLCIVTIGMGEIIRLTLLNNPFGLTGGPNGVFGICFPTV
                     GSLMTIDNCPKFYYYIWIVVGLTVFALIRLQNSRIGRAWNYIREDVIAAEALGVDVRY
                     YKLLAFVLGAALAGVAGNIYATKLMIVSPESFSFMESCLLFCIVLIGGMGSIPGVLIG
                     AAAISLFPELFQTFAQYRMLFFGAVMIIMMMFRPGGVWPRQRGRILLKPVLMRAEKSE
                     I"
     gene            complement(1654..2565)
                     /locus_tag="PKJ95_02395"
     CDS             complement(1654..2565)
                     /locus_tag="PKJ95_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR65125.1"
                     /translation="MKLIIEQLLNGLTIGSFYSLIAMGYSMVYGVMKLINFAHGDFFT
                     FGSYMGYTLLVMGITFITSTLGVWGGMIAAMLIAAISSGVLGLTVERIAYRPIYPAGR
                     LPAVVSALGASIFIQNAIMIIWGPRPHAYTSDVIPGFILNIAGFRINILQIIILLLSF
                     LLMGILYYIVQKTTFGAAIRASALDRETAALMGININLIIFFIFALGPALGGVAGVMV
                     GMYYRKISFIMGWNFGLKAFTATILGGIGNIPGAMVGGLILGVLEMLGSTYLSTAYKD
                     VFVFLVLILVLLFRPTGILGEKIAEKV"
     gene            complement(2598..3746)
                     /locus_tag="PKJ95_02400"
     CDS             complement(2598..3746)
                     /locus_tag="PKJ95_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNR65126.1"
                     /translation="MNRIKFILIVVMVLGVCFSFNVFAQQPPVVIGLQGPLTGAEAIE
                     GEMAKQSVETAARMVNERGGVLGGRMIKIELVDDACQPKAGALAAMKLVSMKEVVAAI
                     ATYGSSVTQPASDIYEKFKKVNIAYGATVTHLSERGLKYFFRTCGRTDTQGIFFADQV
                     VPHFKAKRVAIMHDNTSFALGLAEEARNAALAAGNIDIVYFDAITPGESDYTVPLTKL
                     REKNPEVFYFTGYFPEAGLIIRQAREIGINAVFVGGDAAINEDFIRIAGLEYAAGCYM
                     TQEALIEYFTNPEAMEFKAKYQEAYGSLPSSPWPVYAADALMVLAEAIDKSGSTNSDV
                     LVNYLKHEMKEFPSITGHIGFDEKGDRVGTGINLYVVNPDGSYSIVKI"
BASE COUNT         1290 a          899 c          645 g         1133 t
ORIGIN      
        1 tatcgagaaa gcccataagg tcctcagtct ccttatcatt taaaccgcta ctgggctcat
       61 ccaagatcag taaaccgggc tcactggcca gggctcgggc aatctccagc atcctctgat
      121 taccataagc aatattttta gccagctcat gtttatagca ggacaacccg acctgttcca
      181 atctatcttc agcgattttc attattcttt tttcttcatt cagttgagca ggtgttctga
      241 agatagattg ccacattcct gagttgcccc tggcatgctg accggccata acattctcca
      301 ggcaggtcat atttggaaat agcctgatat tctgaaaggt tcgggcaata cctttggata
      361 aaatctgatg cggctttaaa ccgaataacc tttccccttt aaaataatat tcgccatcat
      421 caggatggta aataccagta atcacattaa atactgtggt tttaccagca ccattggggc
      481 caattatgct actaattttg cctttaagta cttctaaatc gaattgatta actgcaacaa
      541 gtccaccaaa acgcttagtc aaccttttgg tttccagcat aatatctttt tttatatttt
      601 ttacatcatt ttttagcctt tcccctattt tatttattat atctcactct tttctgctct
      661 catcaaaact ggtttaagta gaatccttcc cctctgcctg ggccaaaccc ctccaggtct
      721 aaacatcatc ataataatca ttaccgctcc aaagaaaagc atacggtatt gagcaaaggt
      781 ttgaaacaat tctgggaaaa gagagatggc agcagcccct attaagacac cgggtataga
      841 acccattcct cctatcagga caatacagaa taagagacat gattccataa aactaaaact
      901 ctccggggaa acaatcatta atttggtagc gtaaatattt ccagctaccc ccgctaaagc
      961 agcacctaat acgaaagcca acaatttata atatctaaca tcaactccta acgcttctgc
     1021 agcaataaca tcttctctga tataattcca tgcccttcca atcctcgaat tctgcagcct
     1081 tattaatgca aaaacagtta agccaactac aatccagata tagtaataaa attttggaca
     1141 gttatcaata gtcatcaggc tgccaacagt gggaaaacag attccgaaaa caccattggg
     1201 gccaccggta aggccgaagg ggttgttaag caaagtcagt cgaataatct ctcccattcc
     1261 tatggtaaca atacacaagt agtctccttt taaatgaata atgggagagc agaccagata
     1321 ggcaaaaatt gcagcagcaa tggcgctgat tggcaataga atcaacaccg ggataccaaa
     1381 cctggtattt aaaatggcag tagtataagc accaatagcg gcaaaaccca tatgtcctaa
     1441 gtcaaataaa ccaacttcac ctaaaacaat attcaagctt aagcctaaca aggtatatat
     1501 tccaaagaag aatgccacat caataaaata atcattcttt attaccagag gaaatagaag
     1561 aaataatagt attagtaaaa taacaaacca attatttttt ctactgagtc tatttacctg
     1621 actattttca ttttttaatt tcatactcta tacctaaacc ttctcagcaa ttttttctcc
     1681 aagtatacct gttggcctaa aaagtaatac cagaatcaag accaaaaaaa caaaaacatc
     1741 tttataagca gtggaaagat aggtcgaacc gagcatctcc agtaccccca ggatcaaccc
     1801 tcccaccatc gctccgggaa tattgccaat accgcctaaa atggtagcgg taaaagcttt
     1861 taaaccaaaa ttccatccca taataaaact aatcttgcga taatacatac ccaccattac
     1921 accggctact ccacctaatg ctggaccgag agcaaatata aaaaagatta ttagattgat
     1981 atttatcccc attaaagcag ctgtttctct atctaaagct gaagctctta tggcagcgcc
     2041 aaaggttgtt ttttgaacaa tatagtatag aattcccatt aagagaaaag acagcagtaa
     2101 gatgattatc tgtaatatat taatcctgaa accagctata tttaatataa aacctgggat
     2161 aacatctgag gtataggcat gtgggcgcgg accccagata atcattatgg cattttgtat
     2221 gaatattgag gcacctaagg cagataccac agccggcaac ctgccagccg ggtagatggg
     2281 acgataggct attcgttcta ctgttaaacc taaaacaccg gaactgattg cggctatcaa
     2341 catagccgca atcataccgc cccatacacc taaggtagaa gtaataaagg ttatgcccat
     2401 caccaataga gtgtagccca tataggaacc aaaggtaaaa aagtcaccat gggcaaaatt
     2461 gattaatttc attactccat ataccattga atagcccata gcaattaagg aataaaaaga
     2521 cccaatggtt aatccgttta acagctgttc tattattaat ttcataatgc aaaccttcta
     2581 ccttataaaa taactaatta tatttttact attgagtagc tcccatcagg attcacaaca
     2641 taaagattga ttccggtgcc aacccggtct cctttttcgt caaatccgat gtgtccggta
     2701 atactgggaa attctttcat ttcatgcttt aagtaattca ccaaaacatc cgaattagta
     2761 gaaccgctct tatcaattgc ttctgccaaa accattagag cgtcagcagc atataccggc
     2821 caggggctgg aaggcaaact gccatatgcc tcctgatatt tagccttaaa ttccatagct
     2881 tccggattag taaaatattc aattaaagct tcctgggtca tataacatcc tgcagcatac
     2941 tctaaaccgg ctattcttat gaaatcttca ttgatggctg catctccacc cacaaagaca
     3001 gcattgatac caatttctct tgcctgtctt ataatgaggc ctgcttcagg aaaataacct
     3061 gtgaaataaa agacttctgg atttttctct cttaatttgg tgagtggcac tgtgtaatca
     3121 gattctccgg gagtaattgc atcaaagtag actatatcaa tattccctgc agcaagagca
     3181 gcatttcgcg cctcttctgc caaacctaaa gcaaaggaag tattatcatg cataatagcc
     3241 actctcttag ctttaaaatg tggaactacc tgatcagcaa agaatattcc ctgggtatct
     3301 gttctgccac aggtacggaa gaagtacttt agtcccctct ctgataaatg ggtaacagtt
     3361 gctccatagg caatattaac ctttttaaat ttttcataaa tatctgaggc aggctgtgtc
     3421 accgaagagc cataggtggc aatagctgca actacttctt tcatagaaac caatttcata
     3481 gctgccagtg cccctgcctt gggttgacag gcatcatcca ccaattcgat cttaatcatc
     3541 cttccaccta aaacgccacc tctttcattg accattcggg cggctgtttc cacagactgt
     3601 ttagccattt ctccttcaat agcttcagca cctgttaagg gtccctgtaa accaataact
     3661 accgggggtt gttgtgcaaa aacattaaag gaaaaacata ctcctaaaac catcacgaca
     3721 attaaaataa atttaattct gttcataatt tcctcctttt aaatttgttt aataagaaca
     3781 tggtaatatt ttcctattta ctttgattaa agttatcatc acctcctatt cttatagtag
     3841 atagtggaga caaaatgtta agattcaatt aagcagtgga ttagttttaa agtaaatagt
     3901 atgcaggcat tataccactg atggctctaa aatcaactca ccccgctcaa atcgggtaat
     3961 atgtagc
//
LOCUS       DAOQWT010000079         3964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_23348_length_3964_cov_3.046303, whole genome shotgun sequence.
ACCESSION   DAOQWT010000079 DAOQWT010000000
VERSION     DAOQWT010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23348_length_3964_cov_3.046303"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKJ95_02405"
     CDS             <1..576
                     /locus_tag="PKJ95_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR65127.1"
                     /translation="GSPVEEAMIKTLMEIDGADFSKTEIVTIGAIDFFAAVSEYVDYT
                     WIYYGWDGVASEIKKFPINFIFLQDYAKELDFYTPVIIAREQLLTTEPELVKRFLRAT
                     QKGYLFAIESPKIAIQDLLRYAPEIDREIAIASQEYLAKEYQADASYWGVMEAQRWED
                     YARWLYERDLIPRMLNVVQAFDNSFLPGKEN"
     gene            620..1312
                     /locus_tag="PKJ95_02410"
     CDS             620..1312
                     /locus_tag="PKJ95_02410"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65128.1"
                     /translation="MPIVEDVSFYLHKNEFVTLLGPSGCGKSTILNMIAGIVPVEEGK
                     VYIDGRDCTGEVGNVSYMYQKDLLLPWKKVIDNTILPLIIKGETVGEARKKAIPYLSL
                     FGLEGFEYKYPFQLSGGMRQRAALLRTYMFSKDIVLLDEPFAGLDAITRYNMQSWLMQ
                     VADKVKATILFVTHDIEEAIYLSDRIYVLTERPARIKEEVKIQLSRPRERDIVTSTQF
                     NRIKKHILLNLS"
     gene            1327..2001
                     /locus_tag="PKJ95_02415"
     CDS             1327..2001
                     /locus_tag="PKJ95_02415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR65129.1"
                     /translation="MALFNGEAEDYDKWYETKIGEHADRVETECALSLFKVEPGMRLL
                     DVGCGTGNFSIKLARQGASVVGVDISREMLVIAEKKAEQEKLDIEFKEMDGLNLLFPD
                     ETFDGVLSMATIEFIPDYPEMISEMFRVCKKGGSILVGTINRDSAWGRLYQDPEYQRK
                     ARIFQYAHLRTSEELSRLRSNHLISTRECLFIPPDLPESEISSAKEKELSQNNRGGFF
                     CVLWKK"
     gene            2089..2604
                     /locus_tag="PKJ95_02420"
     CDS             2089..2604
                     /locus_tag="PKJ95_02420"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HNR65130.1"
                     /translation="MRITGNVISGIILLVVGFVLLLANFMGIKVNFFRLFPGIALLIL
                     GIFILFGQIGGRDELIFDHRKIDLSEPFREKNIIFAEGIMDLNDLNLPESSKKIKLNV
                     VFGSGRLILNQDIPTIVHASSVFGNVQLPEQSVTFIESTEYILGEIHSGKPYLDIEAN
                     VIFGQLKIVKP"
     gene            2913..3809
                     /gene="cysK"
                     /locus_tag="PKJ95_02425"
     CDS             2913..3809
                     /gene="cysK"
                     /locus_tag="PKJ95_02425"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNR65131.1"
                     /translation="MLINSITQVIGNTPLLRLEKVSTGNVYAKAEFLNPGGSIKDRVA
                     KYIIEEAEKDQKLRPGMTIIEATSGNTGIGLAFVGVQKGYEVICIMPENMSEERKKIM
                     NAFGGKIVATSAEESLTGSIRKMREMIKEEPERYFVVDQFVNPKNPETHYKILGPEIW
                     EDTKGEIDIFIAGVGSGGTLQGVGQFLKEKNPSIKIVAVEPKNSAALLGKEPGLHQIQ
                     GIGDGFIPGVLNVNLVDFVITVSDEEAIKTTRLLSLKEGLLVGTSSGANVCAALHLDN
                     GRNRVITVLPDRAERYFSTALL"
BASE COUNT         1306 a          626 c          883 g         1149 t
ORIGIN      
        1 ggttcaccag ttgaagaggc tatgattaag actttgatgg agattgatgg tgctgatttt
       61 agtaaaacag agatagtgac cattggtgcc attgattttt ttgccgcagt ctctgaatat
      121 gtcgactata cctggattta ctatggatgg gatggggtag cgtcagagat taaaaaattt
      181 ccaattaact tcatattttt acaggattat gccaaagagt tggattttta taccccggta
      241 ataattgcca gagaacaact tttaacaaca gagccggagt tggtaaaaag atttttaaga
      301 gctacccaaa aaggttatct atttgccata gaaagtccaa aaatagcaat tcaggatttg
      361 ctaagatatg caccggaaat agatagggaa atagcaattg ccagtcaaga gtatttagca
      421 aaagagtatc aggctgatgc gtcttactgg ggtgtgatgg aagcacaacg ctgggaagat
      481 tatgcccgtt ggttatatga gagagacctc atccccagaa tgctgaatgt tgttcaagcc
      541 tttgataatt cttttttacc tgggaaggag aactgatatt aaattaaggg taagtaattt
      601 caggcaacaa tacgaagaga tgcccattgt tgaagatgtc tctttttatc tccataaaaa
      661 tgaatttgta accctgctcg gaccaagtgg ttgcggaaag agtactatac tcaatatgat
      721 agcgggaata gttcccgttg aagaaggaaa agtgtatatt gatggtcggg attgtactgg
      781 agaggtgggc aatgtcagct atatgtatca gaaagacctg ttgttaccct ggaaaaaagt
      841 aattgacaat actattttac ccttaattat taaaggggaa acagtgggag aagccagaaa
      901 gaaggcaatc ccatatcttt ccttatttgg cctggaaggt tttgaatata aatatccttt
      961 tcaattgtct ggaggtatga gacagagggc agcactgcta agaacctata tgttttccaa
     1021 agatattgta ttattagatg aaccttttgc tggattggat gccataacgc gttataatat
     1081 gcaatcctgg ttaatgcaag tggcagataa agtcaaagct accatcttgt ttgtaacaca
     1141 tgatattgaa gaggctattt acctctcaga ccgaatatat gttcttactg aacggccggc
     1201 cagaataaaa gaggaagtga aaattcagct atccaggccg agagaaaggg atattgttac
     1261 ctcaacacag tttaatcgta ttaaaaagca tatcttatta aatctatcct gaaaggagga
     1321 gcatagttgg cactattcaa tggagaagca gaagattatg ataaatggta tgaaactaag
     1381 ataggagaac atgccgatag ggtggagaca gaatgtgctc tcagtctttt taaggttgaa
     1441 ccaggaatgc gcctgcttga tgttggctgt ggaacaggaa atttcagtat aaaattagca
     1501 cggcaaggcg cctctgtggt tggtgttgat atctcacggg agatgttggt aatagcagaa
     1561 aaaaaagcag agcaggaaaa attggatatt gaatttaaag aaatggatgg actcaatcta
     1621 ctatttccag atgaaacatt tgatggagtt ctatctatgg ctactatcga gtttattccg
     1681 gactaccctg agatgatttc agagatgttt agagtctgca aaaagggagg ctctatctta
     1741 gtaggtacca tcaatagaga cagcgcctgg ggcagattgt accaggaccc ggaatatcaa
     1801 aggaaagcaa gaatatttca atatgcccac ttaagaacct ccgaagaact gagcaggctc
     1861 agaagcaatc acttaataag taccagagag tgtcttttta taccacctga tttgcctgag
     1921 tctgagatta gctcagccaa agaaaaagaa ttgtcccaaa ataaccgtgg tggttttttc
     1981 tgtgtactct ggaaaaaata ataataccct tttacttcta ggtattagtg acaatactat
     2041 atattagtta taaaatatag gtaacatgta aaaagagagg agaacaccat gagaataaca
     2101 gggaatgtta ttagtggcat tattcttttg gtcgtaggct ttgtcttatt gctggctaac
     2161 tttatgggaa taaaggtgaa tttttttcgc ctgtttccag ggatagccct tttgatattg
     2221 ggaatattta ttctgtttgg tcagattggg ggaagggatg aacttatctt tgaccatagg
     2281 aaaattgacc tgagtgaacc ttttcgagaa aagaatatca tttttgccga aggaataatg
     2341 gatctcaatg atttaaatct accggaatcg tctaaaaaaa tcaagctcaa tgttgttttt
     2401 ggttcaggca gattaatact gaatcaggat attcctacca ttgttcatgc cagttcggta
     2461 tttggaaatg tacaattacc cgagcaatcg gtaactttca ttgagtcaac cgagtatatt
     2521 ctgggagaaa ttcattctgg taaaccatat ctggatattg aagccaatgt gatattcggt
     2581 cagctaaaaa tagttaaacc ctgatcatcg aataggggac agataggcaa aaatggagtc
     2641 aggttcaaaa gagaacatca ctggggtcag gtctcaacat ttgacattac tttcttatgt
     2701 caaaagttga gacctgaccc tctattatta ttgctattat cgaaacatat taatacacat
     2761 taaagtttta cttgcgaagg gatgataatt ttgatatatt atcgctatta tcatatatag
     2821 cacacacatt aatatgattt attattttca tttctttctt gctgagttac tgtgcaaaac
     2881 aaaagaataa tccaaaatag agaggcagca caatgttaat caatagtatc acccaggtaa
     2941 tagggaatac accacttctc cgcctggaga aggtatcaac aggtaatgtg tacgccaaag
     3001 cggaatttct caaccccggg ggaagtatta aagatcgggt ggcaaagtac atcattgaag
     3061 aggcagaaaa agaccaaaaa ttaagaccgg ggatgaccat tattgaggca acttccggta
     3121 atacagggat aggactggct tttgttggtg tccagaaagg ttatgaagta atctgtataa
     3181 tgcctgaaaa tatgagcgag gaaagaaaaa aaattatgaa tgcctttggt ggaaaaattg
     3241 ttgccacatc tgctgaagag agtttaaccg gttccatcag aaaaatgagg gagatgatta
     3301 aggaggagcc ggaaagatat tttgtggtag accagtttgt caatcctaaa aatccagaaa
     3361 cacattacaa gatattaggt cctgaaatct gggaagatac taaaggtgaa atagacatat
     3421 ttattgccgg tgtgggtagt ggtgggacac ttcagggagt gggacagttt ttaaaggaga
     3481 agaatccatc tataaagatt gtggcagtgg aaccaaaaaa cagtgcagct ctactgggaa
     3541 aagaaccagg attacatcag attcagggca taggtgacgg atttattcca ggggttttaa
     3601 atgtaaatct ggttgatttt gtgataacag tctctgatga agaggcaatt aagactaccc
     3661 gacttctttc cctaaaagaa ggactattag tggggacttc ctcaggagca aatgtctgtg
     3721 ctgcactgca tctggataat ggcagaaatc gagttattac tgttttgccc gatagggcgg
     3781 aaagatattt cagtaccgct cttttataac tataagggca aaatattttt atattattct
     3841 tattgtaaaa caggataaaa gctaaaaatc aattggcagc tttagccttt ttatttattc
     3901 ttataaattg gtccaattga accacaatta ccgaacttat caccattaga attcccatta
     3961 gttt
//
LOCUS       DAOQWT010000080         3959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_23390_length_3959_cov_2.779713, whole genome shotgun sequence.
ACCESSION   DAOQWT010000080 DAOQWT010000000
VERSION     DAOQWT010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23390_length_3959_cov_2.779713"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /gene="rimO"
                     /locus_tag="PKJ95_02430"
     CDS             <1..1279
                     /gene="rimO"
                     /locus_tag="PKJ95_02430"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNR65132.1"
                     /translation="LLKHQYTITNDLENCDIVLINTCSFIEPAVEESIDNILEAVNLK
                     KEGKVHYIVVAGCLPQRYKGQELIKSLPEVDAFIGIDEIPHIADILKKVIKGEKTFQV
                     NLKPSYLYNENSPRFLFTPGHYAYLKIAEGCNNACTYCLIPHIKGPYRSRTIESIFSE
                     AQSLVDRYPLKEIILIAEDTTYYGKDLYGATSLADLLKRLTELNWSRENRIRILYTHP
                     AHYDDKLIDCLVENELFCPYLDIPLQHISNPILKRMNRKIGKDEIIALIDKLRRRIPN
                     LTLRTTFIIGFPGETEADFQELCDFVQDYKFEKVGVLKYYDEADCYASKYPEQVPEKV
                     KKERLDKLMSIQQKIALTHQEGKIGKKIKVLVDGFYENGGNILAGRSCAEAPEIDGNI
                     LILNGKKKDIGNWVEVEISKAYPYHLEGKIRDK"
     gene            1467..2570
                     /locus_tag="PKJ95_02435"
     CDS             1467..2570
                     /locus_tag="PKJ95_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNR65133.1"
                     /translation="MSKERIRKFWQEINKSETRPAAFLVLNPKNIFYLSQFKGEGILL
                     STFEQNYLITDSRYTEQAKQEAQNCIIITQDIKQNDARNIALSKLLAELKIKELGFES
                     NFLKVADYLKFEQTMPRIKLLPFTGIIETIRIIKDQSEIALLEKAAQIANTAFLKTIS
                     RWLAAGISEQALANQLNCNIRKEGASKESFDLIVTSGERGTLIHGEPSDKLIEEGELI
                     IIDFGGIYHNYNSDCTRTVCLGKVDKQQEKIFNIIKEVQIETLRKVKAGMLCSELDKF
                     ARTRIEEEGYGDYFLHSLGHGVGLDIHELPRLSSNDETILESGMVITIEPGIYVPGIG
                     GVRIEDTVVVTEKGCHNLTMLPKELLSTYYLEE"
     gene            2593..3819
                     /gene="glyA"
                     /locus_tag="PKJ95_02440"
     CDS             2593..3819
                     /gene="glyA"
                     /locus_tag="PKJ95_02440"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNR65134.1"
                     /translation="MSIEKVDFEIYQLIKKEEERQKFTLELIASENFVDEAILEAQGS
                     ILTNKYAEGYPGKKYYGGCQFIDEVENLAIDRAKRLFSADHANVQPHSGSQANMGVYF
                     SVLKPGDTVLSMNLSHGGHLTHGSPVNFSGMLYNIVHYGVDRDSGRINYNELKKLAVQ
                     VKPKMIIAGASAYPREIDFAQFRAIADEVGALLLADVAHIAGLIVTGEHSSPIPHCHF
                     VTTTTHKTLRGPRGGLILCEQNFAQKVDKSIFPGIQGGPLMHVIAAKAICFKLAMQDD
                     FITYQKQIKKNASVLAHTLMEREYQLVSGGTDNHLMLVDLRNKNITGKEAEKALEEAG
                     ITVNKNTVPFDTQSPMVTSGIRIGTPAMTTRGMKEKEMVQIAELIDRVLSNIDNDKVS
                     QEVHQDVVKLCQQFVS"
BASE COUNT         1348 a          656 c          828 g         1127 t
ORIGIN      
        1 tctgttaaaa caccaatata ctattaccaa tgatttggaa aactgtgaca tcgtccttat
       61 caatacctgt agttttattg agccagcggt agaagaatca atagataata tcttagaagc
      121 agtaaactta aaaaaagaag ggaaagtcca ctacattgtg gtagctggtt gccttccgca
      181 aagatataaa ggtcaggaac tgataaaatc attgccagaa gttgacgcct ttattggtat
      241 tgatgaaata ccccatattg ccgacatcct aaaaaaagtc ataaaaggag aaaagacatt
      301 tcaggtcaat cttaaaccct cctatcttta taatgaaaat tcgccgcgtt tcctctttac
      361 tcctggccat tatgcctacc ttaaaattgc tgagggttgc aataatgcct gtacttattg
      421 ccttatacct catatcaaag gaccttatcg gagcagaact atagaatcga tattttcaga
      481 agcacagagt ttagtggaca gatatccttt aaaggagatt attctgatag cagaggatac
      541 tacctattat ggaaaagacc tttatggtgc gacctccctg gcagatttat taaaaagact
      601 gactgagctg aactggtcca gagagaatcg cattagaata ctctacactc atccggctca
      661 ttatgatgat aaattaatcg attgtctggt agagaatgaa cttttttgtc cttatctgga
      721 tattcctctc cagcatatca gtaacccgat tttaaagaga atgaatagaa aaattggaaa
      781 ggatgagata atagcactta ttgataaatt gagaagaaga atccccaatt taaccctgcg
      841 tactactttt attataggtt ttcccggtga gacagaggcc gattttcaag aactttgcga
      901 ttttgtacag gactataagt ttgaaaaagt gggggtactt aaatattacg atgaagctga
      961 ttgctatgcc tctaaatatc cggaacaggt tcctgaaaaa gtcaaaaaag aaagattgga
     1021 taaactgatg agcatacagc aaaagatagc tttaactcac caggaaggga aaattgggaa
     1081 aaagataaag gttttagttg atggtttcta tgaaaatggg ggaaatatcc tggcaggacg
     1141 ttcctgtgca gaagcacctg aaattgatgg caacatattg atattaaatg gtaagaaaaa
     1201 agatattgga aactgggtag aagtggaaat cagtaaagcc tatccctatc atctggaagg
     1261 taaaataagg gataaataaa atataatgtt tgcagattaa ggcattttat ggttataata
     1321 tcatacaccc caaaatattg ttctttattt ttttatataa aattctaaaa atacgcgaat
     1381 aatattgtta tattttctat attgccagtg agtaaaaaat aattgggtaa atatgataaa
     1441 attatgataa aaaaggaagc tgaattttga gcaaagagag aataagaaag ttctggcagg
     1501 aaataaataa aagtgaaacc aggcctgctg catttctggt ccttaaccca aaaaatattt
     1561 tttatctttc tcaatttaaa ggagaaggca tactgctttc tacctttgaa caaaactatc
     1621 ttattactga ttcccgttat actgaacagg caaagcagga agcgcaaaac tgtatcatta
     1681 taactcagga tataaaacag aatgatgccc gaaacatagc tttatccaaa ctcctcgctg
     1741 aactaaaaat taaagagtta ggatttgaat ctaattttct taaagttgct gattatctaa
     1801 aatttgagca gactatgccc aggataaaac ttctcccctt taccggcatt attgagacaa
     1861 tccgcattat taaagaccaa tctgaaattg cattactgga aaaagcagcc caaattgcca
     1921 ataccgcttt tttaaaaact ataagtagat ggttagcagc cggtatctct gaacaagccc
     1981 tcgccaatca attgaattgt aatatacgaa aggaaggtgc cagtaaagaa tcctttgatt
     2041 taattgtaac ctccggggaa aggggaacgt tgatacatgg agaaccgtct gataagctga
     2101 tagaggaggg ggaacttatc atcatcgatt ttggcggtat ttaccacaac tacaactcag
     2161 attgcaccag aaccgtttgt ttagggaaag tagataagca gcaggaaaaa atatttaata
     2221 ttattaaaga agtacagata gagaccttaa ggaaggttaa ggcgggtatg ttgtgcagtg
     2281 aactggataa atttgcccgc accagaattg aagaggaagg atatggtgat tatttcttgc
     2341 actctttagg ccatggagtg gggcttgata tacatgaatt accccgtctc agttcaaatg
     2401 atgagactat attagaatcg ggtatggtga ttaccattga acccggtatc tatgtacccg
     2461 gtattggtgg agttcgcatt gaggataccg tagtagtaac agaaaaaggt tgccataacc
     2521 ttactatgtt gcctaaagag ctactatcca catattatct tgaagaataa atagttcaaa
     2581 aggagggaag aaatgagtat agaaaaagtt gattttgaaa tataccagct tatcaagaaa
     2641 gaggaagagc gacaaaaatt tactctggaa ttgattgctt ctgaaaattt tgttgatgaa
     2701 gctatcctgg aagcccaggg ttctatactg accaataaat atgctgaagg ttatcccggc
     2761 aaaaaatatt atggtggatg tcagtttatt gatgaggtgg aaaatttagc cattgaccgg
     2821 gcaaaaagat tattttcagc cgaccatgcc aatgtgcagc cgcattccgg ttcacaagcc
     2881 aatatgggag tctatttttc tgtattaaaa ccaggtgata ccgttctttc tatgaacctt
     2941 tcccatggtg gtcatcttac ccatggcagt ccggttaatt tttcaggtat gctttacaac
     3001 atagtgcatt atggagttga tagggatagc ggacggatta attataatga attgaaaaag
     3061 ctggcggtac aggttaaacc gaagatgatt attgccggtg ccagcgccta ccccagagaa
     3121 atcgattttg cccaattcag agcaatagca gatgaggttg gagctttgtt gctggctgat
     3181 gttgcccata ttgctggttt aatagttacc ggtgagcata gcagcccaat tccacactgc
     3241 cattttgtaa ctaccactac acataagaca ttgagaggac caagaggagg tctgatactc
     3301 tgtgaacaga attttgccca aaaggtcgat aagagtattt tcccgggcat acagggggga
     3361 cctttgatgc atgttattgc ggcaaaggcg atatgcttta agctggctat gcaggatgat
     3421 tttataacct atcagaagca gattaagaaa aatgccagtg ttttagcgca tacattaatg
     3481 gaaagggaat atcagttagt atccggaggg actgataatc acctgatgct ggttgatttg
     3541 agaaataaga acataaccgg taaagaggct gaaaaagcat tggaggaggc aggtataacg
     3601 gttaataaaa ataccgtgcc ttttgacact caatctccta tggttaccag tggcatccgt
     3661 attggtacac cggctatgac cacccgtggt atgaaagaaa aagagatggt acaaattgct
     3721 gaactgattg accgggtact gagtaatatt gataatgaca aggtcagtca ggaggttcat
     3781 caggacgttg tgaagttatg ccagcagttt gtttcatagg caagtgctaa aataaaacca
     3841 attgtcaaaa tagttaattt attaataaaa atagtaaata tatatatatg atatgtgatt
     3901 ggacagtctt ctccaatgca agatgagcct gaaatctgtt cttaaatatt aacatattt
//
LOCUS       DAOQWT010000081         3946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_23492_length_3946_cov_2.980725, whole genome shotgun sequence.
ACCESSION   DAOQWT010000081 DAOQWT010000000
VERSION     DAOQWT010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23492_length_3946_cov_2.980725"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="PKJ95_02445"
     CDS             complement(<1..1380)
                     /locus_tag="PKJ95_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNR65135.1"
                     /translation="MKQSMNYTQNNKNSWLLQQLKNIFIVMIILLMSKLNTMAQVPIL
                     RVGIFLDYKEISLKGDKGLKIFELSTGENLVIQRDNQVFKITAAPEGIKINNQIFKTS
                     KGIKAIPIGDGFLQVQDKKYRGDIEINVNKSLLKVINIIEIEKYLYGVLKKEISPEWP
                     AEVLKAQAIAARTFALSNMNKYINQGYNICATTSSQEYGGISCEHPATSKAINDTRGI
                     VATYEGQPINAVYHSDSGGCTENCEDVWGGYVPYLRSVSSEYEAIVSPPNHHWSYSLT
                     EEELLTKLASNGFQLNSIENVGIGEKTESGRVKLINVIGDNGKQISFKTNDFRLLIGP
                     SLIRSSLFTMESEGGSWIEEEQEEDCIIEEPAKEEPHPQKTVSDILKEDRDFTITELI
                     ALLNRSKELPEPKVIAKAKIMERRKNSDGLIIVFKGKGSGHGVGLSQWGAYGMDTRGF
                     KYDEILKYYY"
     gene            complement(1431..1652)
                     /locus_tag="PKJ95_02450"
     CDS             complement(1431..1652)
                     /locus_tag="PKJ95_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNR65136.1"
                     /translation="MEGPSAFGKILIIIGIMILAAGLIILLLDKLPSFSFRLPGDILI
                     RRKNFVFYFPLGLCILISAILTLLLRIFK"
     gene            complement(1670..>2375)
                     /gene="ruvB"
                     /locus_tag="PKJ95_02455"
     CDS             complement(1670..>2375)
                     /gene="ruvB"
                     /locus_tag="PKJ95_02455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNR65137.1"
                     /translation="FIDEIHRLNRAVEEILYPALEDFALDILIGKGPSARSIRIGLSM
                     FTLIGATTRTGLITSPLRSRFGVISRIGYYNEEDLHQIIVRSASILGIEIEESASREI
                     AKRSRGTPRIANRLLKRVRDYAQIEGKGVISDEIAFFALKNMGIDEQGLCEIDKRLLL
                     TIMDKFKGGPVGLGTLAASLNEDKDTICDVHEPYLLQKGFLTRTPQGRKATEIAYNYF
                     RQNKFRLTGEDNSTLF"
     assembly_gap    2376..2385
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2386..2747)
                     /locus_tag="PKJ95_02460"
     CDS             complement(<2386..2747)
                     /locus_tag="PKJ95_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR65138.1"
                     /translation="MKEKEDLIEKDLKTESLDSETDLRPQKMLYFIGQQNVKNNLSIY
                     IKAALKRNEPMDHILLYGPPGLGKTTLANIIAREMGVNIKMTSGPVIERAGDLAAILT
                     NLKEKDVLYLKFKRLLPE"
     gene            complement(2789..3376)
                     /gene="ruvA"
                     /locus_tag="PKJ95_02465"
     CDS             complement(2789..3376)
                     /gene="ruvA"
                     /locus_tag="PKJ95_02465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNR65139.1"
                     /translation="MISFLTGKLESIEPGTLTINVNGVGYLVYVPISDKFDINEYQQT
                     VYTYLYIREDRMVLYGFVSKDERDFFKLLIDTPGIGPKIALNIISDMGSERFQIAVLS
                     ENLSIISSIPGIGLKMAKKIVLELKEKFKMFKVDQEIIAQEEKKDFIKEGIEALKGLG
                     YSEREAKQKILSTMKNISDQNFTKLEDLIKEALKK"
     gene            complement(3480..>3946)
                     /gene="ruvC"
                     /locus_tag="PKJ95_02470"
     CDS             complement(3480..>3946)
                     /gene="ruvC"
                     /locus_tag="PKJ95_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNR65140.1"
                     /translation="GIDPGLNHTGYGLVGLEKDRIRALDYGCIVNSVSETFTEKIKKI
                     HLEIDSLIKTYSPHEIVLEEIFFSKNIRSALNVGKVVGVVSLTATLLGIDFFVYTPLQ
                     IKLSVVGYGRASKLQVQEMVKILLNLPEKPDSDHAADALAVAICHIHNIHSY"
BASE COUNT         1174 a          815 c          563 g         1384 t
ORIGIN      
        1 ataataatac tttaagattt catcatactt gaatccccgg gtatccatcc cataggctcc
       61 ccattgagaa agtccaacac catgtccact acctttgccc ttaaatacaa ttattaaacc
      121 atcggagttt tttcttcttt ccataatttt tgcttttgca atcacttttg gttccggtaa
      181 ttctttagat cgatttaata aggcaatcag ttctgtaatg gtaaagtccc tatcttcctt
      241 aagaatatca ctcacagttt tttgggggtg gggctcctct ttggccggtt cctctataat
      301 acaatcctct tcttgctcct cttctatcca gctacctcct tcgctttcca tagtaaatag
      361 tgagcttctg attaaacttg gtccgatgag cagtctaaaa tcattagttt taaatgaaat
      421 ctgctttcca ttatctccta taacattgat taattttact ctgccagatt ctgttttttc
      481 accaatacca acattctcaa tactatttag ttgaaagcca ttactcgcta attttgttaa
      541 caactcctct tctgtcaaag agtaactcca gtgatgatta ggcggtgata caattgcttc
      601 atactctgag gaaacacttc ttaaataggg gacatagcca ccccatacat cttcacaatt
      661 ttccgtacag cctccgctat cagaatggta gacagcattg ataggctgcc cctcataagt
      721 ggcaacaatg cctctggtat catttattgc tttactggta gcgggatgtt cacaagaaat
      781 accaccatat tcctgactgc tggttgtagc acagatatta tatccctgat ttatatattt
      841 attcatattg gagagggcaa aggtcctggc agcaatagcc tgagctttta gaacttcggc
      901 aggccattcc ggtgatattt ctttctttaa aactccatag agatattttt caatctcaat
      961 tatgttaatg accttcagga gagatttatt tacatttatt tcaatatccc ctctatattt
     1021 tttatcctgt acctgaagga atccatcacc aataggaatt gccttaatgc ccttacttgt
     1081 cttaaagatt tggttgttga tttttatccc ttctggtgca gcagtaattt taaacacctg
     1141 attgtccctt tgtatcacta aattctctcc agtagaaagt tcaaatatct ttaatccctt
     1201 atcacccttt aagctaatct ctttataatc taagaagatt cctactctta atattggtac
     1261 ctgtgccata gtgttcagtt tggacattaa aaggataatc atcactataa aaatattttt
     1321 taattgttgt agaagccagg aatttttatt attttgagta tagttcatcg attgtttcat
     1381 taaatctgct ccatttggtt aatataattt ttgttagcgg aataataata ctatttaaaa
     1441 attctcaaaa gaagtgttaa aatagcacta atcaaaatac ataatcccag aggaaaataa
     1501 aaaacaaaat tttttctcct tattaaaata tcgccaggta agcggaaaga aaaggaaggc
     1561 aatttatcca gtaataaaat aattaacccg gcagccaata tcattattcc aattataata
     1621 agaattttac caaaagcact cggaccttcc atctctttaa aaagctcctt tagaataatg
     1681 ttgaattatc ttctccagtc aacctgaatt tattctgccg aaaataatta taagcaattt
     1741 cggtcgcttt tctcccttgg ggagtcctgg ttaaaaaacc tttttgcaga agatatggtt
     1801 catgtacatc gcatatagta tccttatctt cattcaggga ggctgccagg gttcccagtc
     1861 caacgggacc acccttaaat ttatccataa tagtcagcag taagcgttta tctatttcgc
     1921 agagaccctg ttcgtcaatt cccatatttt ttaaagcaaa aaaagctatt tcatcactta
     1981 taactccctt tccctcgatc tgagcataat cccgaactct ttttaataat cggttggcta
     2041 ttcgcggggt tcccctggaa cgcttagcaa tttcccttga tgctgattct tctatctcaa
     2101 tacctaaaat tgaagctgaa cggacaataa tctgatgaag gtcttcttca ttataatatc
     2161 ctattctgga tatcacccca aaacgactgc gcaaggggga ggtaatcaag ccagttcggg
     2221 tagtagctcc tatcaaggta aacatagaca aacctatacg tattgaacga gcactgggcc
     2281 ctttaccgat aagtatatcc aaagcaaaat cttctaatgc cgggtacaat atctcttcaa
     2341 cagctcggtt aaggcgatga atctcatcaa taaatnnnnn nnnnnttttc aggaagtaat
     2401 cttttaaatt ttagatataa aacatctttt tcttttaaat tagttaaaat agctgctaaa
     2461 tcacctgctc tttcaataac cggaccggag gtcattttaa tattaacacc catctctctg
     2521 gcaataatat ttgccagagt tgttttgcct aatcctggag gaccatataa taaaatatgg
     2581 tccattggtt cattccgttt tagagcagct ttaatataaa tagaaagatt atttttcaca
     2641 ttttgctggc caataaaata gagcattttc tgtggtctta aatccgtttc agaatctaaa
     2701 ctttccgttt ttaaatcttt ttcaattaaa tcttcttttt ccttcataac ttgattcttc
     2761 tatctcaatt tatttcaaaa tgattatctt actttttcag agcctctttt attaaatcct
     2821 ctaattttgt aaaattctgg tctgagatat ttttcatagt gctcaaaatc ttttgttttg
     2881 cttctctctc ggaataaccc aaacctttca aagcctctat tccttcctta ataaaatctt
     2941 tcttctcctc ttgagcgatt atttcctgat cgactttgaa cattttaaat ttttctttca
     3001 gttctagaac aatttttttt gccattttaa gcccaatacc tggtatacta cttataatac
     3061 tcagattttc tgacagtacc gcaatttgaa aacgctccga acccatatca gagattatat
     3121 tcaaggctat cttgggccca attccgggag tatcaatcag tagtttaaaa aaatcacgtt
     3181 catccttact cacaaaacca tataatacca tgcggtcttc tcttatatat aaataggtgt
     3241 aaacagtctg ttgatattca ttaatatcaa acttatcact tatgggaaca tagactaagt
     3301 aacctactcc gtttacatta atagtaaggg ttccaggttc tattgactct aactttccag
     3361 ttaaaaaaga tatcataatc tctatccttc ccattaatcg agataaataa gcccttcaat
     3421 cctgaaatct ctttttatta aaatcttctt tgtcgggaat tcaatctgcc tttaagtctt
     3481 taataagaat gtatgttgtg aatgtgacag atagctaccg cgagggcatc tgctgcatga
     3541 tcagaatcag gcttttctgg caggtttaac aatattttaa ccatttcctg tacctgaagt
     3601 ttactggctc taccatatcc cactacagat agttttatct gtagtggtgt atagacaaaa
     3661 aaatcaattc ctaacagggt agcagttaag gaaactactc ctacaacctt tcccacattt
     3721 aatgcacttc tgatgttttt actaaaaaat atctcctcaa gaactatttc atgaggagaa
     3781 taagttttaa tcagactatc tatctctaag tgtatttttt ttatcttctc tgtgaaggtt
     3841 tcacttacac tattaacaat acaaccataa tctagggctc ttattctatc tttttctaat
     3901 cccactaatc cgtaaccggt atgattaaga cctggatcga taccta
//
LOCUS       DAOQWT010000082         3909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_23807_length_3909_cov_2.604048, whole genome shotgun sequence.
ACCESSION   DAOQWT010000082 DAOQWT010000000
VERSION     DAOQWT010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23807_length_3909_cov_2.604048"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PKJ95_02475"
     CDS             <1..1026
                     /locus_tag="PKJ95_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNR65141.1"
                     /translation="VQVKEGAFSKNQLVKAQIDNQDRIAIAANHTATHLLHYALREVL
                     GSHVKQAGSSVTSEHLRFDYTHFAPLKKEELDEIEKMVNQKVNENYSITSEITDLNTA
                     QQKGAIALFGEKYGEKVRMIDIGGFSRELCGGTHLDETSRIGIFKIISEQGIGSGLRR
                     IEAVTREKAQESIKKEETLLQELEQILDIPQDRLLQKVNQLLQDNESLKCEYRLLWKN
                     TLPSRLEKIIKEQKVIKGLTVVSAILDTIDKTDLREAGDSIKGKITSGIIILATISKD
                     NRINLIIMLTDDLVDKGYDAGKLIQPIAIALNGQGGGKRHFAQAGGTDLTKLKTIFQN
                     IEQYIQI"
     gene            1372..1824
                     /gene="ruvX"
                     /locus_tag="PKJ95_02480"
     CDS             1372..1824
                     /gene="ruvX"
                     /locus_tag="PKJ95_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNR65142.1"
                     /translation="MRILAIDIGEKRIGLAISDALGITAQGLPTIEIKSEQEIYSKIM
                     EIIKEKNVDHLVFGLPRNMNGTLGPQAKKVQECAQKFEKLSNLPVDFEDERLSTVRAE
                     QVLLEADTSRAKRKKVIDKLAAAIFLQSYLDRKGIEKNENNKNNKSNN"
     gene            1790..2776
                     /gene="mltG"
                     /locus_tag="PKJ95_02485"
     CDS             1790..2776
                     /gene="mltG"
                     /locus_tag="PKJ95_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNR65143.1"
                     /translation="MKIIKTIKAIINKFLNALFFLFLLVFLALLLSAAWVLVPVQQEE
                     PVEKVIEIPYGYNSTEIRQLLEKEGIIRPHNYLFPMVTKMLKIDSRLQSGEYQLSSSQ
                     NLLQIIEQLVKGRVVLYRITIPEGFQHRQIARLLADRKIIDYDAFIELIKENIQYKEG
                     YLFPDTYEFPKNFGVKNVLKAMQKNFEEVVYKHINPEQQFPEGLNFAQVIILASIIEK
                     EALGTEDKPRIASVFYNRLSRGMLLQSCATVQYLLDKPQERLKEKDLAIESPFNTYLY
                     PGLPPEPICNPGLESIMAAAYPAKEDYLYFVLGKEGKHIFSKTYQEHLNNKP"
     gene            2943..3572
                     /gene="udk"
                     /locus_tag="PKJ95_02490"
     CDS             2943..3572
                     /gene="udk"
                     /locus_tag="PKJ95_02490"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNR65144.1"
                     /translation="MLPLIIGIAGGTGCGKTTVAKKIVLGIKGKKAIIIPQDAYYKDL
                     SHLALKERKKFNFDHPAAFDNELLLNHLNNLIQGKPISMPVYSFQDYTRKKEVKKVKP
                     ADVIILEGILVLEEESIREMLNIKIYVDTDSDERFIRRLIRDTRERGRSLSSVVEQYL
                     NRVKPMFLQFVEPSKRYADIIIPQGGLNRVAIDIITSTINARLNQFGVF"
     gene            3638..>3909
                     /locus_tag="PKJ95_02495"
     CDS             3638..>3909
                     /locus_tag="PKJ95_02495"
                     /inference="COORDINATES: protein motif:HMM:NF020972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HNR65145.1"
                     /translation="MPHLFRILVPDVYLENIYSIDTAKLKEKKNIKGIILDLDNTIVP
                     WGTNNLDDRVLQWIEQVKQNGVKVCLVSNTHKNISDIGHKLAIPFL"
BASE COUNT         1381 a          665 c          775 g         1088 t
ORIGIN      
        1 gttcaggtaa aagagggtgc ttttagtaaa aaccagctgg ttaaagccca gattgataat
       61 caggatagaa tcgctatcgc cgccaatcat actgctacac atctcctgca ctatgctttg
      121 cgtgaggtac tgggcagcca tgttaaacaa gccggctcat cagttacatc tgaacacctt
      181 agattcgatt atactcactt tgctcctctg aaaaaggaag agctggatga gattgaaaaa
      241 atggttaatc aaaaagtaaa tgaaaattac tccatcacat cagaaattac tgacttaaat
      301 actgctcagc aaaagggagc cattgcacta tttggtgaga agtatggaga aaaagtacga
      361 atgattgata tcggtggttt cagcagggaa ctttgcggtg gcactcatct ggacgaaacc
      421 tccagaatcg gaatctttaa aattatctca gagcagggaa taggcagtgg tttaaggaga
      481 attgaagcag taaccaggga aaaggcgcag gaaagtataa aaaaagagga aaccctgtta
      541 caggaattag agcaaattct tgatataccg caggatagac tactgcagaa agtcaatcag
      601 ttattacagg ataatgaatc attaaagtgt gagtatcgtt tactctggaa aaatacactt
      661 ccatcccgtc tggaaaagat tattaaagaa caaaaagtta ttaagggact aaccgttgtc
      721 tcggcaattc tcgataccat tgacaagacc gatttaagag aagccggaga ttcaattaaa
      781 ggaaaaataa cttctggaat aattattctg gctaccataa gcaaagacaa caggattaat
      841 ttaattatta tgctcaccga tgatttagtt gataaaggtt atgatgccgg caagctcatt
      901 caaccaattg ccatagctct caatggtcag ggtggaggta agcgtcattt tgctcaagcc
      961 ggtggaactg acctgactaa attaaaaact atatttcaga atattgaaca atatattcag
     1021 atttagaaca tttctgtttt tctactatct aactactgca ttttctttat ttttcctgta
     1081 ttatcattat aataaaataa ataaagttta tagttttcag tttatagtta ttagtaaaga
     1141 ataagtgttg cagtaataaa agtataaagt taccagaaca gtcgttagtc gttggtcgtt
     1201 ggtatttagt gtttagtatt tagtttaaat gaaaaggcga aaatgttggc agaaatgtgg
     1261 gaataataat acaaagagtc tgaagaaaag tgtttcctat gtgttggtta aactgtattt
     1321 taccaaaaac taaaaaccaa aaactaaaaa ctttaataag gagaccatat cttgagaata
     1381 ttagccatag atattggcga aaaaagaata ggactggcta taagcgacgc gttgggaatt
     1441 accgcccagg gactgcccac tattgaaata aagagtgaac aggaaattta ctccaaaatt
     1501 atggaaatca tcaaagaaaa aaatgtagac catcttgtct tcggattacc gagaaatatg
     1561 aacggaacat tgggaccaca agccaaaaaa gtgcaggaat gcgcccaaaa gtttgagaag
     1621 ctcagtaacc tgccggtgga ttttgaagat gagagattaa gcaccgtcag ggcagaacag
     1681 gtacttctgg aggcagacac cagcagggca aagagaaaaa aagtaataga taaactggct
     1741 gctgctattt tcttgcaatc ctacctggac cgaaaaggca tagaaaagaa tgaaaataat
     1801 aaaaacaata aaagcaataa ttaacaaatt tcttaacgcc ttattttttt tattcctgct
     1861 tgtcttcctg gctttgttac tttctgctgc ctgggtattg gtaccggtgc agcaggaaga
     1921 accagttgaa aaggtaatag aaataccata cggctataac agcactgaaa taagacaatt
     1981 actggaaaaa gaagggatta tcagacccca taattatctc ttccccatgg taaccaaaat
     2041 gctgaaaata gactccagat tgcagtctgg tgaatatcag ctcagttctt cccaaaatct
     2101 ccttcaaata atagagcagc tggtgaaagg aagagttgtt ctttatcgta taaccatccc
     2161 tgaaggtttt caacaccggc aaattgcccg tttgctggca gaccgaaaaa tcatagacta
     2221 cgatgcattt atagaattaa ttaaagaaaa tattcaatat aaagaagggt acttattccc
     2281 ggatacctat gaatttccca agaattttgg ggtgaaaaat gtgcttaaag ctatgcagaa
     2341 aaactttgaa gaggtggttt ataaacatat taatcccgag cagcagtttc cggagggttt
     2401 aaactttgcc caggtaataa tacttgcctc tatcatagaa aaagaagccc ttggaacaga
     2461 agataaaccc agaattgctt ctgtttttta taacagatta tcaagaggta tgcttttaca
     2521 atcctgtgcc actgtccagt atttactgga taaaccacag gagagattga aagaaaagga
     2581 tctggctata gaatcgccct ttaatactta tttgtatccc ggactgcctc ctgaaccaat
     2641 ctgtaatccc ggattggaat ctattatggc agcagcctat ccggcaaagg aggattattt
     2701 atactttgtg ctgggtaaag agggtaaaca tattttttca aaaacttatc aagaacacct
     2761 gaataataaa ccataggtac gtgtgggtac aaatgaagga aagtttttag ttaatagttt
     2821 tttgttttta gtaaaaataa atactaaaaa tcattaaaac gtgaatgcgg ttttatacga
     2881 caataaaata acgcataaca ccaaacgaat agagtaccaa ttcaaataaa aaggagtaag
     2941 aaatgcttcc tctcatcatt ggcattgccg ggggtactgg ctgtgggaaa accaccgtag
     3001 ccaaaaaaat agtcctgggc attaaaggta aaaaagccat catcattccc caggatgcct
     3061 actataagga cctctcacat cttgccctga aagagcgaaa aaaattcaat tttgaccatc
     3121 ccgccgcttt tgataatgag ttgctgttaa atcatctcaa taacttgatt caaggaaagc
     3181 ccatatcaat gccggtctac tcatttcagg actatacccg taaaaaagaa gttaaaaagg
     3241 ttaaaccggc cgatgtaatt atcctggaag gtattctggt tttggaagag gagagtattc
     3301 gcgaaatgct aaatataaaa atttatgttg ataccgattc agatgaacgt tttatccgca
     3361 gactgatacg ggataccaga gagaggggaa gaagtctttc ctcggtagta gagcagtatc
     3421 tgaacagagt caagcctatg tttttacaat ttgtggagcc ctcaaaacga tatgctgata
     3481 ttattatacc ccagggaggt ttgaacaggg tagcgattga tattattacc agtactatta
     3541 atgcccgttt aaaccaattc ggcgtatttt gactatcata atttaaagaa aaatatttac
     3601 cttgaattat taaaataaag cgataaaagg agttttgatg cctcatcttt ttagaatact
     3661 tgttcctgat gtctatctgg aaaacattta ctccatagac acagcaaaat taaaagaaaa
     3721 gaaaaacata aagggaatta ttttagacct tgataacact attgtaccct gggggacaaa
     3781 taatcttgac gatagggttt tgcaatggat tgagcaggtt aaacagaatg gggtaaaggt
     3841 ttgtcttgtt tccaatactc ataaaaatat ttcagatatt ggccataaac tggctatacc
     3901 ttttttata
//
LOCUS       DAOQWT010000083         3869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_24192_length_3869_cov_3.252491, whole genome shotgun sequence.
ACCESSION   DAOQWT010000083 DAOQWT010000000
VERSION     DAOQWT010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24192_length_3869_cov_3.252491"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PKJ95_02500"
     CDS             complement(<1..651)
                     /locus_tag="PKJ95_02500"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNR65146.1"
                     /translation="MRKKIFLLAVLFVLLFLVNAFASLAREDRCTIMAVGKNATVDGS
                     AIVSYNCDCTDCDFRMFLIPAMDWPEGSMRNIISWGHWNTRMYDYSKKWEIDYGDKVV
                     GEMPEASHTYRYIHTIDSNINEKGVIIGESTCVISANTDYAKEVEKVLQIDSKGIIDY
                     DFAMDIVLERSSTAREAVEVLAYLIETYGWVDDCGECFNITDGNEIWIFEVYGLNLW"
     gene            complement(1267..2457)
                     /locus_tag="PKJ95_02505"
     CDS             complement(1267..2457)
                     /locus_tag="PKJ95_02505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR65147.1"
                     /translation="MRIAFFTNCYKPLINGVVSSIVSLKESLEKKGHETFIFAPRMEA
                     YQDEESNIFRYHSINLTHKVKYPVAIPLSLRAGQVINKFKPDIIHLHHPFVLSLPAIM
                     YAAKLKIPKVLTIHTQYERYAYYVSPIPHIITHEAIRRIIFNLSDKVDIITTPSQSMK
                     DLILNYKIKKEITVIPNAIDISIFQNINRQEVELLKKELHITPDDVVIIYVGRVSLEK
                     NIDKIIKALALIRNKKIDNFVFIVVGEGTAVKKLNSLADSLKLREKVRFVGAIAREMV
                     RYYYQIGDIFAFSSTSETFGMVIIESLASGLPVLAVKAPGAVDIIQDGFDGILVEDDV
                     SQFAEQLENLIQNKDLRKKLSLNGLKTVQRYSIEVVSDQVLALYGRLLSDKSKTTDHV
                     SPHH"
     gene            complement(2516..>3869)
                     /gene="glnS"
                     /locus_tag="PKJ95_02510"
     CDS             complement(2516..>3869)
                     /gene="glnS"
                     /locus_tag="PKJ95_02510"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HNR65148.1"
                     /translation="KEGNAYVCDLSAEKIKEYRGTFTKPGVESSCRFRTVDENLTLFQ
                     KMREGLFEEGSRTLRAKIDMSSPNLNMRDPVLYRILKESHHRTGDEWCIYPMYDYAHP
                     LEDYYEGITHSVCTLEFEDHRPLYEWVLKNVHAIDPPQQIEFARLNLSNTVMSKRKLR
                     FLVEKNYVSGWDDPRLPTISGLRRRGYTPEAIRNFCDRIGVAKTNSIVDVRFLEHCIR
                     EDLNKRALRAMAVLNPLKLVIDNYPEDLIEEMDCENNPETPSAGTRKVAFSKELFIER
                     EDFHENPPSKYYRLGPGREVRLKNAYIVKCTNFVREEKSGEIKEIHCIYDPETRSGRP
                     GAERKVKSTLHWVSARFALKAEVRLYDYLLLDSIEGVEQAEEEGDKDFVRLLNPKSIV
                     VLKNSLIEPDLKNLVSGDKIQFMRKGYFCVDPDSTSDNLVFNRIVSLRDSWEKIQKRN
                     "
BASE COUNT         1175 a          795 c          587 g         1312 t
ORIGIN      
        1 ccaaagattt aacccatata cttcaaaaat ccatatttca ttgccatctg taatgttaaa
       61 acattctccg cagtcatcaa cccaaccata tgtttctatt aaatatgcta aaacttccac
      121 tgcttcccga gcagtgctgc ttctttctaa aacgatatcc atagcgaaat cataatcaat
      181 gattccttta ctatcaattt gaagtacttt ctctacttct tttgcatagt ctgtattggc
      241 actgatgaca catgttgatt ctcctataat tactcctttt tcattaatat tcgagtctat
      301 ggtgtgaata tagcgatagg tatgcgaagc ttcgggcatt tcaccaacta ccttatctcc
      361 ataatcaatc tcccattttt ttgaataatc atacatcctg gtattccaat gcccccaact
      421 tataatgttc ctcattgaac cttcaggcca atccattgca ggaataagaa acattcgaaa
      481 atcacaatca gtacagtcgc aattatatga aacgattgca gaaccatcaa ctgtagcatt
      541 tttgccaaca gccataatag tacatctatc ctctcgtgcc aaagaagcaa aagcatttac
      601 taaaaacaat agaacaaaga gcactgccaa taaaaatatc ttctttctca ttaccattac
      661 ctccaaagaa ataataaata attcttcaaa ttgatattat aaatttggat tattaatcac
      721 cctcctttca gtgtattcta ttttgtgctt attaatctcc tccttccgta ttgtatttaa
      781 aaatttcaat atacctcaat ttctttggtt ttgaattttt ttgtaaaaaa acatttacca
      841 gaaagtttac cgaaatacaa aatacaatac ttttagtatt tttaatttgg tgcttgattc
      901 tgcaatccta actttttaaa gaatagacat ctaaaaattt gaactacttt tttggtagtt
      961 tggcagaatt aatgtgttgg ttggctgttt ttttcaaaga aatcattgat atttgtttgt
     1021 tcttcattga acaaaacact gtatatttat caaaatccta tcaattgcac ggaaaaatgt
     1081 caaaaaattt ttatgtactt tcaatcgatt tttctgcact tactattgcc atttcggtat
     1141 ataatatatt ttattactat tttaataaat tcatttgctc aagtgtaatg gaagaacgaa
     1201 actatgacag aatctctttc acttcttccg ctacagtttt attaaaatta ttgataaaaa
     1261 caggattcag tggtggggag agacgtgatc agtagtttta gatttatccg acaatagtct
     1321 cccataaagt gcaagtacct gatcagatac aacctctata ctgtagcgct gcacagtctt
     1381 taatccatta agagataact tctttcttaa atccttgttt tgaataaggt tttctaattg
     1441 ttctgcaaat tgagaaacat catcttctac caaaattcca tcaaaaccat cctggataat
     1501 atcaacagca ccaggggctt tcacagccag aaccggcaaa cctgaggcta atgattctat
     1561 tattaccatc ccaaatgttt ccgaggtaga gctaaaagca aaaatatcac caatctgata
     1621 gtaatatctc accatttccc gagcaatggc tcctacaaac ctaacctttt ctcgcaattt
     1681 taaagaatct gccagactgt taagtttctt cactgcagtt ccttctccta ccacaataaa
     1741 tacaaaatta tcaattttct tattccgtat aagagcgagt gctttaatta ttttgtcaat
     1801 attcttttct agagacactc tacccacata aataatcacc acatcatcgg gggttatgtg
     1861 cagctctttc ttaagcaact caacttcctg tctatttatg ttttgaaaaa tactaatatc
     1921 aatagcatta ggtatgactg tgatttcttt tttaatctta taattgagaa taagatcttt
     1981 catagattgt gaaggggttg ttataatatc tactttatct gacagattaa aaataatcct
     2041 tctgattgcc tcgtgggtaa tgatatgagg aatgggggat acataatagg catatcgttc
     2101 gtattgagtg tggatagtta aaactttagg aattttcagt ttagcagcat acataatggc
     2161 cggcaaactg agcacaaaag ggtggtgaag gtgaataata tcaggcttaa atttattaat
     2221 aacctgtcct gctctcaaag ataatggaat tgccacagga tatttaactt tgtgggttag
     2281 attgatagag tgatatctga atatattgct ctcttcatcc tgataggctt ccattctggg
     2341 ggcaaagata aaagtttcat gacctttctt ttcaagcgat tctttcaaag agacaatact
     2401 gcttaccacc ccattaataa ggggcttata gcaattggta aaaaaagcta ttctcattgt
     2461 catcccttta attgcctgat agtgttaata attatccaaa ccaatcatta attctttaat
     2521 ttcttttctg tatcttttcc cagctatccc gcaaagagac tattctatta aaaaccagat
     2581 tatcagatgt ggaatcaggg tctacacaaa aatacccttt tctcataaat tgaatcttat
     2641 cacctgatac caaattcttc aaatccggct ctataagact attttttaat accactattg
     2701 attttggatt gagcagtctc acaaagtctt tatccccttc ctcttcagcc tgttctaccc
     2761 cttcaatact atcaaggaga aggtagtcat ataggcgcac ctcagctttc agcgcaaacc
     2821 gggcagaaac ccaatgcagg gtacttttca ccttacgttc tgctcccggt cttccacttc
     2881 tcgtttccgg atcatagata cagtgtattt ccttaatttc accgctcttt tcttcacgca
     2941 caaaattagt acatttaact atgtaggcat tctttaatct gacctcacgt cctggtccca
     3001 gacgataata ctttgaaggt gggttttcat ggaaatcttc tctttctata aataattctt
     3061 tagaaaaagc tacttttctg gtaccggctg aaggggtttc agggttattt tcacagtcca
     3121 tctcttctat cagatcttcc ggataattgt caattactaa tttcaaagga tttaaaacag
     3181 ccattgctct tagggctctc ttgtttaaat cttccctgat gcaatgttct aaaaaacgta
     3241 catctactat gctattggtc tttgctactc cgattctatc acagaaattt ctaatagctt
     3301 ccggagtata accgcgtctg cgtaaacctg aaatagtagg cagacgaggg tcatcccaac
     3361 ctgagacata gtttttctct accagaaacc tcagttttct tttactcatc acggtattgc
     3421 ttaaatttag acgggcaaat tcgatctgtt ggggagggtc tatggcatgt acattcttta
     3481 atacccattc atacaaaggg cgatggtctt caaactctaa agtgcatact gagtgagtga
     3541 ttccttcata atagtcttcc agcggatggg cataatcgta catcggatag atacaccatt
     3601 catcaccagt acgatgatga gattctttga gtatccggta tagaaccggg tcacgcatat
     3661 ttaaattagg ggagctcata tcaattttgg cccggagagt acgggagcct tcctcgaata
     3721 aaccctctct catcttttga aaaagagtga gattctcatc tacagtacgg aaacggcagg
     3781 agctctctac ccccggttta gtaaaagtac ctctgtattc ctttatcttt tctgcactta
     3841 agtcgcatac ataggcattc ccttcttta
//
LOCUS       DAOQWT010000084         3856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_24306_length_3856_cov_3.050513, whole genome shotgun sequence.
ACCESSION   DAOQWT010000084 DAOQWT010000000
VERSION     DAOQWT010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24306_length_3856_cov_3.050513"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="PKJ95_02515"
     CDS             complement(<1..1455)
                     /locus_tag="PKJ95_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HNR65149.1"
                     /translation="MNKFWQFLKNNRNNQGSSSWSLEYFKFEPGQEKLRESLCALGNG
                     YLGTRGAFNEVSSSRIHYPGTYLAGVYNKLESNIAGKTIINEDLVNCPNWLPLTFKTD
                     QDQEWIIPSVNNIISYQEKLDFKEASLTRNYRIKENDKKITTVETRRIIHMGNNHLAA
                     QEYILIPENYDGEIIIRSALDGSIENKGVDRYSELNSLHLSAGATGIFSEKGIYLTVT
                     TSQSNIQIAMASTIYLSHNGKEVKPISITTRKEEKTIFQEYRIKVRKKERYVIEKTVA
                     IYTSRDKDTENPLKSAIDLIQEVSGFSSLLDSHKEAWSNLWNMFDIEIKGHIFSEKTI
                     RLHIYHLLQTASVHTSKLDVGIPARGLSGEAYRGHIFWDEIFILPQYNYRLPEITKST
                     LLYRYRRLKQAREYAKENGYQGAMFPWQSGSTGEEETQTIHLNPQSGKWGPDYSRNQR
                     HISFAIAYCIWEHWIITGDLDFMCNYGAELFLSIA"
     gene            complement(1424..3058)
                     /gene="otsB"
                     /locus_tag="PKJ95_02520"
     CDS             complement(1424..3058)
                     /gene="otsB"
                     /locus_tag="PKJ95_02520"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF014418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNR65150.1"
                     /translation="MKKKLAFASAIFDLDGVVTKTATIHAHSWKKVFDEYLRLREKRD
                     GEPFKEFTYQRDYLIYVDGKPRYKGVQNFLESRNIHLPFGNSNDSPDQETICGIGNKK
                     NKLFDKLLQKEGAEVFQSTINLIKDLKNKGIKVGVASSSKNCLAVLKSAHLEHLFETR
                     VDGNVSQKLNLKGKPEGDIFVTAARYLNSHPENSVVVEDASSGVEAGRNGGFGLVLGV
                     ARENNEIELAKHGADIVVTDLSEISVDLINDWFLRKPKDFFLCYEDLKKAPEIFPREH
                     LNSKNILINPYYKRTGKSVIDIKKKMTFFLDYDGTLTPIVESPELAILSPDMKKIVED
                     LANIHTVAIVSGRMREDVQNLVGIEGIFYAGSHGFDIEGPGGFSMIQQEAKKTIPLIS
                     LITNQLKEKLNNIDGVLIEEKKFSVAVHYRKVKKLTDLPLIEEVVNNIIQKYNELRLL
                     KGKKVFEILPNIDWDKGKAVRWIMNVLDITWKSTAVFYIGDDTTDEYAFRNIITRGTA
                     IIVSDELSKTSASDFQLKSPEKVKDFFEQVLAISKK"
     gene            3392..>3856
                     /locus_tag="PKJ95_02525"
     CDS             3392..>3856
                     /locus_tag="PKJ95_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNR65151.1"
                     /translation="MYRITMSLCKNLSKYGQRQKFGHTLFVLLTACSFLFILLISNVF
                     SSEPSVLLGNEVLLQEDYFHLIEGKKVGLITNQTGVDSKGVSFIDKILSTHRVKLIAL
                     FAPEHGLDGKTRAGTQLSSSIHPLYDIPVYSLYGNTRIPTEEMVFSVEVLIFD"
BASE COUNT         1141 a          730 c          635 g         1350 t
ORIGIN      
        1 cgctatagag agaaagagct ctgctccata gttgcacata aagtccaggt cacccgttat
       61 tatccaatgt tcccatatac aatatgctat ggcaaaagat atgtgtctct gattacggct
      121 gtaatcaggt ccccatttgc ccgattgagg attaagatgg atagtttgtg tttcttcttc
      181 ccctgtagaa ccactttgcc aggggaacat ggcaccctga taaccatttt ctttagcata
      241 ttctcgggct tgtttcaatc ttcggtaacg atagagcaga gttgatttgg ttatctccgg
      301 taaacgataa ttgtattgtg gtaaaataaa aatctcatcc cagaagatat gtccgcggta
      361 agcctcccct gacaatcccc tggcaggaat gcccacatct aattttgaag tatgtacaga
      421 tgcggtttgt aagagatgat aaatatgcaa ccttatagtc ttttctgaaa agatatgtcc
      481 ttttatttct atatcaaaca tattccatag attggaccag gcttctttat gagaatccag
      541 cagtgatgaa aagccggata cttcttgtat taaatctata gcacttttta aagggttttc
      601 agtatcttta tcccgggagg tatatatagc aacagttttt tctattacat acctctcttt
      661 tttcctaact tttatcctgt attcttgaaa aatagttttt tcttctttcc gggtagtgat
      721 agagataggt ttcacttctt taccattatg cgataaatat atggttgaag ccatagcaat
      781 ctgtatattt gattgactgg tagtgacagt taaatagata cccttttcag aaaaaattcc
      841 tgttgctcct gcagatagat gaagagagtt aagttcactg tatctatcta cccccttatt
      901 ttcaatactg ccatctaaag cggagcggat aataatttca ccatcgtaat tttcaggaat
      961 aaggatatat tcctgtgctg ccagatgatt atttcccata tgaattatac gacgggtttc
     1021 tacagtggta attttcttgt cgttttcttt aattctataa tttcttgtta atgatgcttc
     1081 tttaaagtcc aatttttctt gataggaaat gatgttgtta acagaaggta ttatccattc
     1141 ctgatcctgg tcagttttga aagttaacgg taaccagttg ggacaattaa ccaggtcctc
     1201 attgataata gttttcccgg caatgttact ttctaatttg ttgtacaccc ctgccaggta
     1261 agttcccggg taatgtatcc tggatgaaga aacttcatta aaggcacctc tggtgcccaa
     1321 ataaccatta cccagagcac ataaggattc ccgtaatttt tcttgacctg gctcaaattt
     1381 gaaatattct aaagaccagg aggaagagcc ctggttattt ctgttatttt ttagaaattg
     1441 ccaaaacttg ttcaaagaaa tcttttacct tttccggtga ttttaactga aaatcagaag
     1501 cagaggtttt tgatagttca tccgaaacta taattgcagt acccctggtt ataatattac
     1561 gaaaggcata ttcatcagtt gtatcatccc caatatagaa aactgctgtc gatttccagg
     1621 ttatatcaag gacattcata atccatctga ccgcctttcc cttatcccaa tctatattag
     1681 gaagaatttc gaagactttt ttacctttta aaagtctcaa ttcattatat ttctggatta
     1741 tattattaac tacctcttca ataaggggta aatcagttaa tttttttacc ttacggtaat
     1801 gaactgctac actaaatttt ttctcttcaa ttagaacccc atctatgtta ttcagctttt
     1861 ctttcagttg atttgtaatc aaagatatta agggaattgt tttttttgct tcctgttgaa
     1921 tcatactaaa gccaccaggc ccttctatat caaaaccatg actgcccgcg taaaaaattc
     1981 cctctatccc caccagattc tgcacatctt ctctcatcct gccactaaca atggcaacag
     2041 tatgaatatt agctaaatct tctactattt ttttcatatc aggggaaaga attgccaatt
     2101 ccggactttc tacaatgggt gtcaaagttc cgtcataatc taaaaaaaag gtcatttttt
     2161 tcttaatgtc aataacagat ttaccggtac gtttataata gggattgatg agaatatttt
     2221 tactattgag atgttcccgg ggaaatattt ctggtgcttt cttcaggtct tcataacaca
     2281 aaaagaaatc tttcggtttc cttaaaaacc agtcatttat taaatcaaca ctaatttccg
     2341 acaggtcagt tactactata tctgctccat gtttagccag ttctatctca ttattctctc
     2401 tggctacacc taaaactaaa ccaaatcctc cgtttctacc ggcttcaact ccggaggagg
     2461 catcctccac taccacagaa ttttcaggat ggctgttcag atatctggca gcagttacaa
     2521 aaatatctcc ctccggtttt ccctttaagt ttagcttctg agatacgttt ccatctactc
     2581 tggtttcgaa taagtgttcc agatgggcgg attttaagac tgcaagacag ttttttgaag
     2641 aagaagccac tcctacctta atccccttgt tctttaaatc cttaataaga ttaatagtgg
     2701 attggaaaac ctcagctcct tctttttgta gcaatttatc aaataattta ttctttttat
     2761 tgccaattcc acaaattgtt tcttggtcgg gcgaatcatt cgagtttcca aagggtaggt
     2821 gaatattccg tgattccaga aagttttgta cacctttata acgaggcttc ccgtcaacat
     2881 agatgaggta atccctctga taagtaaatt ccttaaatgg ttccccatct cttttttctc
     2941 ttaatctaag gtattcatca aagacttttt tccaggagtg ggcatgaatt gtagcagttt
     3001 tggttactac accatccagg tcaaatattg cggaggcaaa ggcaagcttt tttttcataa
     3061 ttacccccta atttttctat cttccttttt ggaattaagg gaagtaagag ataccagagt
     3121 taattaatga taaatgatta tttaaagaag aattcaagaa ctggattcta caattgttag
     3181 ataaaataat actggattaa ttctttattg tcaattcagc atcctgtgta ttattgtatt
     3241 attgcttacc atcattatta taatcttgca atattatcca atatatgtca tattaacaaa
     3301 atataaaagt atatttttag atatataata ttagaaaaat tttcagtaaa aacacctttg
     3361 aaaagattct aaaaaagatg aggaatagtc catgtataga attactatga gtttatgtaa
     3421 gaatttatca aaatacggtc aaagacaaaa attcggccat accttatttg ttttactgac
     3481 tgcctgttca tttttattca ttttattgat aagcaacgtc ttttccagtg aaccttcagt
     3541 cctgctgggc aacgaagttc tgttacagga ggattatttt catcttattg aaggtaaaaa
     3601 ggttggatta ataactaatc aaactggtgt ggatagcaaa ggggtaagtt ttattgataa
     3661 aattctttcc acacacaggg taaaacttat tgcccttttt gcacctgaac atggattgga
     3721 tggtaaaacc agagcaggca ctcagctaag ctcatctatc catcctcttt atgatatacc
     3781 tgtttatagt ctttatggta ataccaggat acctactgaa gaaatggtat tttcggtaga
     3841 agtccttatt tttgat
//
LOCUS       DAOQWT010000085         3848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_24389_length_3848_cov_3.055102, whole genome shotgun sequence.
ACCESSION   DAOQWT010000085 DAOQWT010000000
VERSION     DAOQWT010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24389_length_3848_cov_3.055102"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..1103
                     /locus_tag="PKJ95_02530"
     CDS             303..1103
                     /locus_tag="PKJ95_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65152.1"
                     /translation="MVERREKIQKKLELITRRWWFFLFFIIIQFIPPYTSKSYEWSEI
                     GVVNLEILSNSIVKAWAAWYPISKIIPIILVVSIIFLRNRVSRIFSIYVAITYVLFAF
                     LQNISITEKYGLGVIIINLVMFNLAAIFWFWEAIIGKNDFTSPKQLCWRYWIIPFAFL
                     AFWYPINFGKPDFNPLFLFNNVAGLTFCMMTPVYLTILILYHPKVNMVTLRVTSLVGL
                     IIAFYNLMVNFFMDPSFLWWNGILHLPLLIISAYGLILSFRTMLSRTN"
     gene            1475..1954
                     /locus_tag="PKJ95_02535"
     CDS             1475..1954
                     /locus_tag="PKJ95_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNR65153.1"
                     /translation="MKYVCTLIAVMDLEKSKEFYYDILEQKVIQDFGANVLLSGGFAL
                     QTLETWKDFIEAKEEEILFGGNDSELAFEVDDMDGFVSKLRSFGVTYVHPMKEHSWGQ
                     RVVRFYDPDKHVIEVGENMAVVVKRFIDSGLTAEETAKRMDIPMEYVNYLLTKQNNN"
     gene            1965..2147
                     /locus_tag="PKJ95_02540"
     CDS             1965..2147
                     /locus_tag="PKJ95_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002823615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65154.1"
                     /translation="MEKHKISDYWNMGTAIGLCAAVGIALGALLHNVILWLGIGAGVG
                     VVLGAISAQSKKKKKL"
     gene            2337..3239
                     /locus_tag="PKJ95_02545"
     CDS             2337..3239
                     /locus_tag="PKJ95_02545"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNR65155.1"
                     /translation="MRKIKNKIKSSRKWQLSILGVLIAIGIIVFWYLQANPVVPQMAI
                     YFLNEINLPEPDDKVLIFSPHPDDETIACGGYINESMKRGVDVHIVLVTDGNKHGLKD
                     LRYKEFENATHSLGVLKENLVYLNYPDGELASVNYLELKSNLLEQIKKIEPDIFLYPH
                     PNDTHKDHSTVGKVVEDILQEMKEKAELKQHLEGNKEIVKEEAWKELEVLESLTQKIS
                     GYKYLVHHPHFPHPKKYAPDLFALPPFDIVAIGDRWEVFMLSEDTEKIKEKALKEYVS
                     QLKNPFLHGLLEASIRKNEIFVED"
     gene            3309..>3848
                     /locus_tag="PKJ95_02550"
     CDS             3309..>3848
                     /locus_tag="PKJ95_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HNR65156.1"
                     /translation="MNKISIRFFDDREVRAIWDDKESKWWFSVLDIIGVLRDEDDYQK
                     NRNYWKYLKTKLKREGNQLVSHTNQLKIRAADGKKYLTDTLDYNGIIELAKNFPGKQA
                     NRFIEWFIHSDETIDGKSKSKAYALFDSSLLDTIEVGTTKGLQQIHGYLFGGLYDFAG
                     QIRTQNIAKDGFQFATARFL"
BASE COUNT         1333 a          569 c          740 g         1206 t
ORIGIN      
        1 aatttccaaa gattatgagg taaggaaaaa atacccacaa gttgttgaca ctaacggttt
       61 tgaacttagt cttgatggtt taataaaata aatttaaaat gaattttatt atttcgaaaa
      121 aagaattaaa tcgtaatata tattttcttc ttaaaaaacc aatttcagcc agatcaggga
      181 aaaatcaaac catagttgat tatattttcg gcgttgttat gattatgatg agtttattaa
      241 tttttaccct gggtttggga tttttgtatt aacaaattac atttaaagga ggtaggtaaa
      301 cgatggttga acggagagaa aagattcaaa aaaagttaga actaataact cgaagatggt
      361 ggttttttct tttttttatt attatccaat ttatcccccc ttacacctca aaaagttatg
      421 aatggtcaga aataggggtg gttaatttag agatattaag taattctata gtaaaagctt
      481 gggctgcctg gtatcctatt tctaaaataa tccccattat cttggttgtt tctataatct
      541 ttcttagaaa tcgagtttcc cgtatattta gtatctatgt agctattacc tatgttcttt
      601 ttgcctttct acaaaatatc tcaataacag aaaaatatgg tcttggagtg attataatta
      661 atctggttat gtttaatctg gcggcaattt tttggttctg ggaagcgatt attggtaaaa
      721 atgattttac ttcaccaaaa cagctatgct ggagatattg gattattcca tttgcctttc
      781 tggctttttg gtatcctata aatttcggga aaccagattt taatccccta tttttgttca
      841 acaatgtggc gggtctgact ttttgcatga tgaccccagt atatctaacc atacttattc
      901 tctaccaccc taaggttaat atggttaccc tgagagtcac cagcctggtt ggattaatta
      961 ttgcctttta taatctaatg gtcaatttct ttatggaccc cagtttttta tggtggaacg
     1021 ggatactaca tttaccatta ttaatcatat ctgcctatgg tttaattctt tccttccgta
     1081 ctatgctatc cagaactaac tgaggctatt ggaaaaaaat gaaacagcag gttaaataga
     1141 tgatggagca ctgcaatctt tccgtaccag cgatcgtgtt gcagtggata tgcagggtaa
     1201 ggaaacagag gcagattggt cggcatattt tagcgattat cacactgtga atggaatcct
     1261 acagccgggt atactgcaat cggtttggca ttaccaagat ggagatgttg tttactttaa
     1321 caaaaacaaa tctacagttg atttccaata taaataacat gagtgatttg taaatatctt
     1381 gagaaaggaa tcattaataa aaaatgtatt taacaactga aattaaccgt atagtaattc
     1441 ggtatagcaa aacaaaccaa gggggtttaa aagaatgaaa tatgtttgca cacttattgc
     1501 ggtaatggac ttagagaagt ctaaagaatt ttattacgac atactggaac agaaagttat
     1561 tcaggatttt ggcgcaaatg ttttgttatc tggaggtttt gcactacaaa ctttagaaac
     1621 ctggaaggat ttcatagagg caaaggaaga agaaatttta tttggaggta atgattccga
     1681 gttggctttc gaagtagatg atatggatgg gtttgtatca aagttgagga gttttggggt
     1741 tacctatgta cacccaatga aggaacactc ctgggggcag cgggtagtgc gcttctatga
     1801 ccctgataaa catgtaatag aagttggcga aaatatggct gttgtagtga aaagatttat
     1861 tgatagtggg ctgacagctg aagaaactgc taagcgtatg gatataccga tggaatatgt
     1921 taattatctg cttacaaagc agaataataa ttaggaggaa tattatggaa aaacacaaga
     1981 tttctgatta ctggaatatg ggaacagcaa ttggactctg tgctgcagtc ggaatagcac
     2041 ttggtgcgtt gctgcataat gttatattat ggcttggtat aggagcagga gtaggtgtgg
     2101 tattgggagc aatatcagcc caatctaaaa agaagaaaaa attataaaca aatatgagta
     2161 ttgtactgat tatataacta ttcttgcttt acacaatact tagacaatac taatctagca
     2221 caaatgaaaa atcatcatat ggatacttta tgtatataaa caatattttc acattttcgg
     2281 gataacaaat ataatataga aaaagaacag ttttaaaatt aaaatttatg gaaacaatga
     2341 gaaaaataaa aaacaaaatt aaatccagtc gaaaatggca gctatcaata ctgggtgttc
     2401 tcatagcaat tggaataata gtattctggt atcttcaagc taacccggta gtaccgcaga
     2461 tggctatcta ctttttgaat gagataaatt tgcctgaacc ggatgataaa gtattgattt
     2521 tttcacccca tcccgatgat gaaactattg cctgtggtgg atatattaat gaatctatga
     2581 aaagaggagt agatgttcat atcgttttag ttaccgatgg taataagcat ggattaaaag
     2641 atttacgtta caaagaattt gaaaatgcca ctcactcact tggtgtactc aaagaaaatc
     2701 ttgtttatct taattatcct gatggtgaac tggcatcagt taactatctt gagcttaaaa
     2761 gtaatctgtt agaacagatt aaaaagattg agcccgatat tttcttatat cctcatccta
     2821 atgatacaca taaagaccat tctacagttg ggaaagtagt agaagacatc ttacaggaaa
     2881 tgaaagaaaa agcagaattg aaacagcatc tggaaggaaa taaagagata gtaaaagaag
     2941 aagcatggaa agaattggaa gtattggaaa gtcttacaca aaagatctct ggttacaagt
     3001 acttagtcca tcatccccat tttccacatc ccaaaaaata tgcgcccgat ctatttgcat
     3061 tacctccttt tgatatagtt gccataggcg atagatggga ggtatttatg ctttctgaag
     3121 atactgaaaa aataaaagaa aaagccctga aagaatatgt aagccagcta aaaaatccat
     3181 ttttacatgg ccttttagag gcttccataa gaaaaaatga gatatttgtt gaagattaaa
     3241 caaagcaaaa tactttatca atctggttaa taaaatgctt attcttaatt aaatgtaagg
     3301 aaattataat gaacaaaatt tccattcgct tttttgatga tagagaggta cgtgccattt
     3361 gggatgataa agagtccaaa tggtggtttt ctgtgcttga tatcatcggt gttctacggg
     3421 atgaggatga ttatcagaaa aatagaaatt actggaaata cttaaaaaca aaattaaaaa
     3481 gagaaggcaa tcagttggtt agccacacta accaactcaa gattagggca gctgacggga
     3541 aaaaatattt aacagacact ctcgactata acggcatcat agaactggca aaaaattttc
     3601 caggcaaaca ggcaaatcgt tttattgaat ggttcataca cagtgatgaa accattgatg
     3661 gaaaaagcaa atcaaaagca tatgcgcttt ttgacagctc ccttcttgat accatagagg
     3721 tcggtactac caaaggttta cagcaaatcc atggatattt atttggcggg ctatatgatt
     3781 ttgccggaca aatcagaact caaaatattg caaaagatgg ctttcaattt gcgactgcac
     3841 gattttta
//
LOCUS       DAOQWT010000086         3838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_24467_length_3838_cov_3.577584, whole genome shotgun sequence.
ACCESSION   DAOQWT010000086 DAOQWT010000000
VERSION     DAOQWT010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24467_length_3838_cov_3.577584"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKJ95_02555"
     CDS             complement(<1..192)
                     /locus_tag="PKJ95_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR65157.1"
                     /translation="MKKWRSIGNKVIPLSFSTLLIVFWQLIVERGVIARYILPSPWDV
                     VLVCARILPEIKEHIYTTLQ"
     gene            complement(164..454)
                     /locus_tag="PKJ95_02560"
     CDS             complement(164..454)
                     /locus_tag="PKJ95_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HNR65158.1"
                     /translation="MAKITLGLQVLPYVEEERLYQVIGEVISYIASCDVKYEVGPMET
                     VMEGELDQLLEIVKKAQEICIEQGATRVISGIKIDYKKTGVTIDEKVEKYRK"
     regulatory      complement(524..625)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(713..1615)
                     /locus_tag="PKJ95_02565"
     CDS             complement(713..1615)
                     /locus_tag="PKJ95_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HNR65159.1"
                     /translation="MEGWILYKQRQSEISTEKYEMNRFLQVAEQRGISLKILCPEQFD
                     LLVNRDDNKSVIIDDNYTQLPQFLLPRQGASTPYFSLAVIRHLERLGVNTFNSSQSIE
                     IVKDKLYTQQILAADNFPVPRTMLARYPLNIQLIKETIGFPIVVKTISGSQGSGVFLC
                     EKPGHLEDLMQLLDTAKTQANFIIQEFVKTSKGHDVRVFIVGGRVVACMERIARYGTF
                     KANFSRGGKVKAFPISKEIEWLATDCARVLNLDIAGIDLLFDGESFKICEANSSPGFK
                     GIESCCDISIPDVIFDFIKIRLGI"
     gene            complement(1673..2104)
                     /locus_tag="PKJ95_02570"
     CDS             complement(1673..2104)
                     /locus_tag="PKJ95_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNR65160.1"
                     /translation="MKISSLDFEHEGYIPEKFTCDGEDISPNLIIEDIPEGAKRLALI
                     VEDPDAPAGIWIHWIVFNIHITDVIEEGEVPGTQGFNDFRKLEYGGPCPPSGTHRYFF
                     KLYALDTKLDLEEGCRKKDLVKAMEGHILVEAELIGLYQRE"
     gene            2570..3313
                     /locus_tag="PKJ95_02575"
     CDS             2570..3313
                     /locus_tag="PKJ95_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR65161.1"
                     /translation="MKNILEIKDLSLSLNGKKILNHINMEIWEGYVHAIVGPNGAGKS
                     TLASTIMGLEGYRHYTGSIYFKGEDISKLSIDERARLGITFGWQEPARYEGLLIRDFL
                     KAAAGNKNNDFISEQLERVGLDPEEYADRAVDKTLSGGERKKVELASILVMDPALVLL
                     DEPDSGIDVGTLDKMFEVIKEQKKKKRTIVLITHSLAVLKQADHAFLFCNGEILEKAV
                     AKKMIPYFKDKCIPCKHKNIPDKEGVRLE"
     gene            3306..>3838
                     /locus_tag="PKJ95_02580"
     CDS             3306..>3838
                     /locus_tag="PKJ95_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HNR65162.1"
                     /translation="MNNINLAKELAQITETDDIFKQPDIAHIFIHHHKVIQSHLLPGL
                     KVDVKEFEDHIEVNTTVEKNSKIEKPVHMCFGMIPETGIQKMVIKNEIQENASASFLS
                     HCAFPNAVDIQHIMDGTITVGKNARFSYLERHVHGLHGGLKIYPHSKIQLSEGAHFYS
                     EFELIKGRAGFIDIDYEA"
BASE COUNT         1228 a          721 c          654 g         1235 t
ORIGIN      
        1 ctgcagggtg gtataaatat gttcttttat ctcgggcaga atcctggcac ataccagaac
       61 aacatcccat ggcgaaggca ggatatatcg ggcaattaca cctctttcta caatcaattg
      121 ccagaaaact atcaaaagag tactaaagga gagaggaatt accttatttc ctatacttct
      181 ccactttttc atcaatggtc acacctgtct tcttataatc gatcttaata cctgatatta
      241 cccttgtggc cccctgttca atacatatct cctgtgcctt tttaacaatt tctaataact
      301 ggtcaagctc accttccata accgtttcca taggaccgac ttcatactta acatcgcagg
      361 aagcgatata ggatattact tcacctatta cctgataaag cctttcttcc tccacatagg
      421 gcaaaacctg taatcctaaa gtaattttag ccatcttaac ctccaaaaaa aatcgctatt
      481 acctgaagcc acaggtaata gcgatataat ttaactatat tattaaaatt aatagttact
      541 tccctccgct agaattacct aggtcaggtt cggggggtca aagcaaaatg ctttttctca
      601 gcacatagct cccccagtaa ctttccagta ttcttcaatc attacatttt tattgtagca
      661 ctttagttct attttgtaaa gaattcccgc taaaagtgga actgtattat tcttaaatcc
      721 ctaatctaat ttttataaaa tcgaagatga catcaggaat actgatatca cagcagcttt
      781 caatcccttt aaaacccggc gaggagttgg cttcgcatat tttaaaactc tccccatcaa
      841 ataacaaatc tataccggcg atatctaaat ttaataccct ggcacagtcg gtagctaacc
      901 attcaatctc cttacttatc gggaaagcct ttactttacc accacgagaa aaattggctt
      961 taaaagtacc ataacgggct attctttcca tacaggcaac taccctaccc cccacaataa
     1021 ataccctaac atcatgacct ttacttgttt taacaaattc ctggataata aagtttgcct
     1081 gggttttagc ggtatcgagt aattgcataa ggtcttccag atgccctggt ttttcacaca
     1141 aaaaaactcc acttccctgc gaaccagaga tagttttaac tactatagga aatcctatgg
     1201 tttccttaat caattgaata ttgagagggt atcgggctaa catagtacgg ggaacaggga
     1261 aattatcagc agctaatatc tgttgtgtgt ataatttatc ctttactatt tcaatgctct
     1321 gtgatgaatt aaaagtgttt acccctaatc tttccaggtg tcgaataact gccagggaaa
     1381 agtaaggtgt actggctccc tgtctgggca acaagaattg aggcaattga gtataattat
     1441 catctattat gacactttta ttgtcatccc gattgaccag taaatcaaat tgttccgggc
     1501 ataatatctt taaacttatt cctctctgtt ctgctacctg taaaaaacga ttcatttcat
     1561 atttttctgt tgatatctca ctttgtcgct gcttataaag aatccagcct tccatttatt
     1621 tgtctcctgc ttgtagtttc ttaaaaattt ttctaacttt aagaaacaag atttattccc
     1681 gttgataaag accaatcaac tctgcctcta ccaggatatg cccctccata gcctttacta
     1741 aatctttttt acgacagccc tcttccaaat caagtttagt atccagggca tatagtttaa
     1801 agaaatagcg atgggttccc gatggtgggc agggacctcc atattctaat ttccgaaaat
     1861 cattaaatcc ctgagtgcca ggaacttcac cctcctcaat cacatctgtt atatgaatat
     1921 taaaaacaat ccagtgtatc catatgcctg ccggtgcatc agggtcttca actataagtg
     1981 ccagcctttt tgctccctcc ggaatatctt caataataag gttaggactg atatcttctc
     2041 catcacaggt aaatttctcc ggaatatatc cttcgtgttc aaagtcaaga ctacttattt
     2101 tcatttttct aacctcctaa actaaaatat tgattccttt gtttcttaac ctaaaataat
     2161 attttaaaac acagtttgtt tttatacatt ataaaacaaa atttataaaa ttgaaaactg
     2221 ttaaaataaa ccagccgaga aaatgagttg ctattctatt ggcaaatatg ttatattctg
     2281 agatataaaa aatcaaatta atatatgtgt ataagtaaaa tttgattgca aaatagttgt
     2341 tggccgttag tgccaatatg ccaataggtt tatagtttgt ggtttttagt taataatgat
     2401 aatttgatat ataagtactc tgtctgaatt atgttttact tatcacttat tacttggtat
     2461 taatcattat tttatatgcg gataagtaaa ttgtattacg attttgtatt ttgtatagct
     2521 aaaattaatc gatgtaggat tttaaaattg aaagatatgg tatagttcta tgaaaaatat
     2581 attggaaatt aaagatttat ccctttcttt aaatggtaaa aaaatattaa accatattaa
     2641 tatggaaatc tgggaaggtt atgtgcatgc catagtaggt cccaatggtg ccggtaaatc
     2701 cacattggct tctaccatta tggggctgga aggttaccga cactacaccg ggagtattta
     2761 cttcaaagga gaggatatta gcaagctcag cattgatgaa cgtgccagac tgggtattac
     2821 ctttggttgg caggagccag cccgttatga aggattactc atcagagatt ttttaaaggc
     2881 agctgccggt aataaaaata atgattttat ttcagaacaa ttagaaaggg taggattgga
     2941 tcctgaagaa tatgccgatc gcgcagtgga taagacctta agtggtgggg aaagaaaaaa
     3001 ggtagaactg gcctccatct tagttatgga tcccgctctg gtcttattag atgagcctga
     3061 ttcgggtatt gacgtgggca ctctggataa aatgtttgaa gtaattaaag aacaaaagaa
     3121 aaagaaaaga actattgtgc ttataaccca cagtttggca gtattaaaac aggctgacca
     3181 tgcttttctt ttctgtaatg gagaaatctt agagaaggct gtggcaaaaa agatgatacc
     3241 ttattttaag gataagtgta taccatgtaa acataaaaat attcctgata aagaaggagt
     3301 gcgacttgaa taatatcaat ttagctaaag aattagctca aataacagaa actgatgata
     3361 tctttaaaca gcctgatatc gcacatattt tcattcatca tcataaggtt atacagtccc
     3421 atcttttacc gggcttaaag gttgatgtta aagaatttga agatcatatc gaggtaaata
     3481 caacagtaga aaaaaatagc aaaattgaaa aaccggtaca tatgtgtttc ggaatgatac
     3541 cggagacagg cattcaaaaa atggtaatca aaaacgagat tcaggaaaat gcctcggctt
     3601 cttttctatc ccattgtgct tttcctaatg cagttgacat tcagcatatt atggatggta
     3661 ctattacagt tggtaaaaat gcccgattta gttatttgga aagacatgtt cacggtttgc
     3721 acggaggact taagatttat cctcatagta aaattcagct ttcggaaggt gcacattttt
     3781 actcggaatt tgagctgatt aaaggtcgtg ccggttttat tgatattgat tatgaagc
//
LOCUS       DAOQWT010000087         3814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_24702_length_3814_cov_5.347965, whole genome shotgun sequence.
ACCESSION   DAOQWT010000087 DAOQWT010000000
VERSION     DAOQWT010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24702_length_3814_cov_5.347965"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PKJ95_02585"
     CDS             complement(<1..610)
                     /locus_tag="PKJ95_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR65163.1"
                     /translation="MEDIKQISILSDFLSKKVKEKGWSKREFAEKTGLNETYVYKLLS
                     WKREFTPNDETLENISKVLELSEKDSKYLLDLAARERETGNIIYEDVPAPPPVPPEPG
                     PDPEPPEPVPPPDPPHPGPPLPDVDQNTYFEIVHEKLRQDKFVLRNDNIDGLNVVVAT
                     KQEFQLLWFGTQMNIFVIMGYADNISREVIESFSRHAIDYAIK"
     gene            1048..1731
                     /locus_tag="PKJ95_02590"
     CDS             1048..1731
                     /locus_tag="PKJ95_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65164.1"
                     /translation="MEGNGTLTILEASKVTGKSITTIHRYVKKGLLHVKTCYSSGKQV
                     LTVEKEELKRVFNIHDITCYNMPKHDVKTCYNNELQVETNIITKEALKETFKELFEEQ
                     KLQLFKPIEDQSLYRLGRIEQENLFLKQRLETVLCDYKELQEKIKALPDPQIEIEKEN
                     LITVLKDQLTELHKEKETFKLQAEEEKEELLRTYEAKLQKAESKQIEISEAWKKELEQ
                     TKRPWWKLW"
     gene            2029..>3814
                     /locus_tag="PKJ95_02595"
     CDS             2029..>3814
                     /locus_tag="PKJ95_02595"
                     /inference="COORDINATES: protein motif:HMM:NF017826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HNR65165.1"
                     /translation="MNLLENLYKSLSHEKIWGPDGLTPLNNFKANSGGYTACCPNPAH
                     PEKKPSFLMKEGIPAGKCQSCGYHLTWFDAVAIQSGHKDCRVKGRDFWQVLNNLANLD
                     GGLQLELTAEDKAKYETQHEIQDLYNNLSKYLHIQLLESTWAESTRKYLTQREIPVNY
                     LPRFPMLGFYPSIETIEQYLLSEGFSLEIIEKSNVLQKCFQNNCLIFSYKDELGNTLG
                     FKGRKPSLKIKDIKYQKGFKGEIKDRAIMGLECCSIAVKDSTKAVCFEGEFDWLTAQT
                     ATLKQYDRTAEFICFGGSDVKPTKLETLKKAGIKILYLSFDPDQAGKKATREAIKYSE
                     QLNLCPFVINLPAGLDPDEFIKSQGLEAFKNRAKNASTAGEWLKKEFLQQYPDLKKEN
                     LEKAKVDLLEEAQNHKGLVLDSFLQSTSKELNIDNELIRQIIEKQYQKLHSITEAKPL
                     KKSLPDIPAQEEFLIPDNWILNKNGLVKLVQTKEGIKEIAIAPAPFYISSRSANIDTG
                     QEKLELTYLRDDLWKTSYEERSILMDHRKIVQLSSMGFPVDSLNCKEMVNFIHNFEAI
                     NDKILPKRSTISGFGWKREKGVPFFVMPG"
BASE COUNT         1422 a          710 c          666 g         1016 t
ORIGIN      
        1 tctttatcgc ataatcaatt gcatgcctgg agaaactctc tatcacttcc ctggagatat
       61 tatccgcata acccataatt acaaaaatat tcatctgagt tccaaaccac aaaagctgaa
      121 actcctgttt ggttgcaaca acaacattta acccgtcaat attgtcattc cttaaaacaa
      181 atttatcctg tctgagtttt tcatggacaa tttcaaaata agtattttga tcaacatccg
      241 gcagaggcgg acctggatgc ggtggatctg gagggggtac cggctcagga ggttctggat
      301 ctggtccagg ttcaggagga actggaggcg gtgccggcac atcttcataa attatattcc
      361 cggtctccct ctctctggca gcaagatcca gaagatattt actgtccttc tctgagagct
      421 ccagcacttt agaaatattc tctaaagtct catcattagg tgtaaactct cttttccagg
      481 ataaaagctt atatacataa gtttcattca aaccggtctt ttctgcaaac tctcttttgc
      541 tccagccctt ttccttaacc ttcttactta aaaaatcact caatatgctt atctgtttta
      601 tatcttccac gtcataatac ctctctcttt tattttttaa gaattattca atacatacaa
      661 aactttatcc tttaaagcct caaataacca attcaataca gataaacact gacaaaataa
      721 gcaaaaaaat ctggcacgga caataattca tctgcacaaa atctggcacg ataaaaagat
      781 tgtatgacaa gatcatgaca gatctggcac gagcattaat tttaaccctg ctgaatctgg
      841 cacgatagta aatttaataa cattaaacct ggcacgatgt taaattttgt cattttctgt
      901 ttttatacgt tgccagattc gcatgaagcc ctttagataa aagcaggaaa ctttattgat
      961 ctgtggaata tagcaaatag aacaggtgaa gatgttctga aacagagcaa ttcacctgtt
     1021 ttattatttc tctggaggaa tgaaattatg gaaggtaatg gaacattaac aatattagaa
     1081 gcctctaaag taacaggaaa aagcattacc actattcaca gatatgttaa gaaggggtta
     1141 ttacatgtca aaacatgtta tagcagtggc aaacaggttt taactgtaga aaaagaagaa
     1201 ttgaaaaggg tttttaatat acatgatata acatgctata acatgccaaa acatgatgtt
     1261 aaaacatgtt ataacaatga attacaggta gaaacaaata ttattactaa agaggcatta
     1321 aaagaaacat ttaaagaatt atttgaagag caaaaattgc aattatttaa acccatagaa
     1381 gatcaatcac tttacaggct tggaagaata gaacaggaga atttattctt aaaacagaga
     1441 ttagagactg ttctttgtga ttacaaagaa ttacaagaga aaataaaggc acttccagat
     1501 ccacaaatcg aaatagagaa agaaaactta ataacggtac ttaaagatca actaacggag
     1561 cttcacaagg agaaagaaac ttttaaatta caggcagaag aggaaaagga agaacttctt
     1621 agaacatatg aggccaaact ccaaaaagca gaatccaaac aaattgagat atcggaagcc
     1681 tggaaaaagg aacttgaaca aacaaaaagg ccctggtgga aattatggta aaagatattt
     1741 tagaaataaa tcaacataaa gaggattata aatactaaaa atagaatcaa tctgtgggtt
     1801 cttattatgg ggtaaggaat atagagtaaa aaagttggct aaaaaacaag aaatcgagtc
     1861 tggaacactc gatttcagag gtaaaccaac atctttaggc aaaaaatttt aattgctctc
     1921 acctccttac ttttaagatg taggaggtat tttataccat tactatatta attatataaa
     1981 ttataacaaa atcctgcaaa tatatagaga aaaaggagaa attcaatcat gaacctttta
     2041 gaaaatttat ataaatcgct ctcccatgag aaaatatggg gacccgatgg actcacccct
     2101 ttaaacaatt tcaaagccaa ttccggcggt tatactgcct gctgcccgaa cccggcacat
     2161 ccagaaaaaa agccatcttt cctcatgaaa gagggcatac ctgccggcaa atgccagtcc
     2221 tgcggatatc acctcacatg gtttgatgca gtagccatcc aatcagggca taaagactgc
     2281 agggtaaaag gcagagactt ctggcaggtt ttaaataatt tagcaaacct tgacggaggt
     2341 ttgcaattag agctgactgc agaagataaa gcaaaatatg aaactcagca tgaaatccag
     2401 gatctttata acaacctgtc aaaatatctt cacatacagc tcttagaaag cacatgggca
     2461 gaaagcacaa gaaaatatct tacacagagg gaaatccctg ttaattatct tccaagattt
     2521 cccatgcttg gcttttatcc ctcaatagaa accatagaac aataccttct ttcagaaggc
     2581 ttcagccttg aaataataga gaaaagcaat gtattacaaa aatgctttca aaacaactgc
     2641 cttatattct cctataaaga tgaattggga aacactttag gattcaaagg cagaaaacca
     2701 tctcttaaaa tcaaagatat caaataccag aaaggtttta agggagagat aaaagacaga
     2761 gctatcatgg gtttagagtg ctgcagcata gcagtaaaag acagcacaaa agccgtatgt
     2821 tttgaaggtg aattcgactg gctaactgct cagacagcca cactcaagca atatgacaga
     2881 acagcagaat ttatatgttt tggcgggtcc gatgtaaaac ctactaaact ggaaaccctt
     2941 aaaaaagcag gaataaaaat tctatattta agtttcgatc cagaccaggc aggcaagaaa
     3001 gccaccagag aagccataaa atattctgaa cagcttaatc tatgtccctt tgtaatcaat
     3061 cttcctgccg gattagatcc agacgaattc ataaaatccc agggattaga ggcatttaaa
     3121 aaccgtgcta aaaatgcttc tacagccgga gaatggctta aaaaggaatt tctccagcaa
     3181 tacccggact taaagaaaga gaacctggaa aaagcaaaag tagatctgct tgaagaggcc
     3241 cagaatcata aaggacttgt actggattct ttcttacaat ccacatctaa agaattaaac
     3301 atagataatg aacttatccg ccagataata gaaaagcaat accagaaatt acatagtatt
     3361 acagaagcaa aaccattaaa gaaatctctg ccggatatac cggctcagga agaattcctt
     3421 atccctgata actggatatt aaataaaaat ggacttgtca aactggtcca aacaaaagaa
     3481 ggtattaaag aaatagccat agctccggct cctttttata tatcttcaag atccgccaat
     3541 attgatacag ggcaggaaaa acttgaacta acctacctgc gtgatgatct gtggaaaacc
     3601 tcctatgagg aaagatccat tctcatggat cacagaaaaa tagttcagct ttcaagcatg
     3661 ggatttcctg tggattccct taattgcaaa gaaatggtta atttcataca caattttgaa
     3721 gccatcaatg acaaaatact tccaaaaaga tcaactatct ccggatttgg atggaaaaga
     3781 gaaaaaggag ttcctttctt tgtaatgcct ggaa
//
LOCUS       DAOQWT010000088         3812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_24731_length_3812_cov_3.190045, whole genome shotgun sequence.
ACCESSION   DAOQWT010000088 DAOQWT010000000
VERSION     DAOQWT010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24731_length_3812_cov_3.190045"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..539)
                     /locus_tag="PKJ95_02600"
     CDS             complement(9..539)
                     /locus_tag="PKJ95_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNR65166.1"
                     /translation="MNVLSLFDGISIARLALKNLNIDCTYFSSEIDKYCIQISKKHFP
                     EIIRLGDVRTVLGKDLPRIDLLIGGSPCQDLSIAKRGRKGLSGEKSGLFYEFLRILKE
                     VNPDYFLLENVSSPECIQISELLQIKPVQINSSLFTAQMRNRLYWTNIKFPYPKDKNI
                     NLSDVSMLLFSTPDNP"
     gene            complement(536..1354)
                     /locus_tag="PKJ95_02605"
     CDS             complement(536..1354)
                     /locus_tag="PKJ95_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65167.1"
                     /translation="MKHIIQFSGGLASAEVARIIIDMYGKGNVILMNHDVKIEHPDTK
                     KFKEDVCNYLGVEYVEVSDGRDLWDLIVDQKCLPSLWIPFCTRILKQKQGLKFCKSLK
                     DKFILYNGFGVHESRRTSGSIRECSKRGIIVKSPLQEYNIPDIEVMRMIRDEWKIQIP
                     EPYMNGFEHNNCLPCFKGGKAHFKRVWQFYPGSFEKAKWAEEKIGHTVFKLKRKTGRV
                     IIKNGEKCHEYITIRQPLSELEKRWAEESVEKKVMTFNHADLWQQMDMFEEVGV"
     gene            complement(1351..1557)
                     /locus_tag="PKJ95_02610"
     CDS             complement(1351..1557)
                     /locus_tag="PKJ95_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65168.1"
                     /translation="MTQLTLFPPPTYAELCREEAKVFFRCVVCFKKRKRGTGIKDKFC
                     SEKHERIYQKWEEELEEVKRRIEG"
     gene            complement(1554..2075)
                     /locus_tag="PKJ95_02615"
     CDS             complement(1554..2075)
                     /locus_tag="PKJ95_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNR65169.1"
                     /translation="MKLLSLDLSTTSTGYAVFRDNQLESSGTIALRIKDWKERIKEIY
                     QIVYHLILKEKHPYEVIEVVTEKPLSLQNGDTTIKLAKLHGLILSICFQEKIELYEID
                     NQTWKKHIAGAKASKEQTKKHLEIFYQYKTETFDEADAIGVGLGWMAEQRLKEKMEKY
                     APVKRMRKSRKAA"
     gene            complement(2164..>3812)
                     /locus_tag="PKJ95_02620"
     CDS             complement(2164..>3812)
                     /locus_tag="PKJ95_02620"
                     /inference="COORDINATES: protein motif:HMM:NF011539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HNR65170.1"
                     /translation="ETTGLDPHTCKIRLIQIGCEGQPVLLVDCFKVNPEPVKEILSDV
                     SIKKCLQNAKFDLKFLRTHLLPVRGKLFDTMIAAQLINSGVKEKCSLDALSKKYLGID
                     LSKENQKSNWSNDPLTQEQIEYAAMDAEILLPLRLKLCEKLYEMCLLKTAEIEFNCLY
                     PVAEMELSGIKLDIEKWNILKAEYQEKKESLSKEILSLIHGKTSEENLDMFGENAVNL
                     DSPAQLKEQLNKIGFPVSDTSFETLARLQNIPLIAKILDYRSVSKALSSFLVPLPEQI
                     HKKTKRIHSTYWQTGSDAGRFSCSDPNLQQIPRTKDFRSCFIPHGGNKFVIADYSQIE
                     LRVAAEISGDETMVEAYSGGADLHRLTAQMVSGREEVTKEERQLAKALNFGLIFGMGA
                     ARFVEYARNSYGVTMTLDQAEEFKAKFFNGYKGLKKWHNKTKREKPMETRTLSGRRRV
                     FSENEEYFQLTARLNTPVQGTAADICKMALGLLYEAIKPVGARIVGTVHDEILVEVKE
                     EKAEETKQILSDCMVRAGQEFLKSVPVLADANVCNNWSDK"
BASE COUNT         1020 a          831 c          610 g         1351 t
ORIGIN      
        1 ctactggatc atggattatc tggtgtgctg aacagcaaca tggacacatc agagagatta
       61 atatttttat ctttcgggta tggaaatttt atatttgtcc agtacaatct atttctcatt
      121 tgagctgtaa aaagagagga attaatctgc actggtttta tttgaagaag ttctgaaatc
      181 tgaatacatt caggacttga tacattttct aaaagaaaat aatctggatt tacttctttt
      241 aaaattctta gaaattcata aaataatccg cttttctctc ctgatagtcc tttgcgcccc
      301 ctttttgcga tagacaaatc ctggcacggg gaacctccaa ttaataaatc tattcgaggt
      361 aagtcttttc ctaaaacagt tctcacgtca cccaggcgaa ttatctctgg aaagtgtttt
      421 tttgaaatct gaatgcagta tttgtctatc tctgaagaaa aataagtaca gtctatattc
      481 agatttttca gagctaaccg ggctatagat ataccgtcaa atagacttag aacgttcaca
      541 ctcccacctc ctcgaacata tccatctgtt gccacaaatc tgcatgatta aaagtcatta
      601 cctttttctc taccgactct tctgcccatc tcttctctaa ctcagataat ggctgtctga
      661 ttgttatgta ttcatggcat ttttcaccat ttttaattat tactcttcca gtttttcttt
      721 tgagtttaaa tactgtatgt cctatctttt cttctgccca tttcgccttc tcgaagctcc
      781 ctggatagaa ctgccatact cttttaaagt gggccttacc gcccttaaaa caaggtaagc
      841 agttattatg ctcaaatcca ttcatatatg gctctggtat ttggattttc cattcatccc
      901 ggatcattct cattacctct atatccggta tgttgtattc ctgaagagga gatttgacta
      961 ttattcctct tttactgcat tcccttatgc tccctgaagt tcttctgctt tcgtgaactc
     1021 cgaacccatt ataaagaatg aatttgtctt tgagagattt gcaaaattta aggccctgtt
     1081 tttgtttcaa aattcgagtg cagaaaggta tccagagaga aggaaggcat ttctgatcga
     1141 ctattaaatc ccagaggtca cgtccgtcgc ttacctccac atattcaact cccaaataat
     1201 tacagacatc ttctttaaat tttttcgtat ctggatgttc tatttttaca tcatggttca
     1261 taagaattac attcccttta ccatacatat ctataattat tcttgctact tcagcactgg
     1321 caagaccgcc tgagaattga ataatatgtt tcatccctct atcctcctct ttacctcttc
     1381 cagctcctct tcccacttct gatatattct ctcgtgtttc tcagagcaga atttatcttt
     1441 tattcccgtg cctctcttcc gctttttgaa acaaacgaca cagcgaaaga aaacttttgc
     1501 ttcttctcta caaagttcgg cgtaggtggg tggggggaag agggttagtt gggtcatgcc
     1561 gctttcctgc tctttctcat tctctttacc ggtgcgtatt tttccatttt ctcttttaat
     1621 ctctgttctg ccatccagcc aagccctacg ccgatagcgt cagcttcatc aaaagtttca
     1681 gttttatact gataaaaaat ttctaaatgt tttttcgtct gctctttact tgcctttgct
     1741 ccagctatgt gctttttcca ggtttgatta tcaatttcat ataattctat tttttcttga
     1801 aaacagattg aaagaataag gccatgtaat ttagcaagtt ttattgtagt gtctccgttt
     1861 tgaagagaaa gaggtttttc tgttacaacc tcaataacct cataaggatg tttttctttt
     1921 aaaatcaagt ggtatactat ctgatatatt tcctttattc tctctttcca gtcttttatt
     1981 cttaatgcta ttgttccgct ggattctaat tggttatctc gaaaaacagc atatcccgtg
     2041 gatgtggttg aaagatctag agataataat ttcataactc ctcctgatac cttttgcaat
     2101 catcataata aggacattca aaaggacatt cttcttctaa aaaataaccg aaaaaccgac
     2161 acattattta tcactccagt tattacaaac atttgcatct gccaaaaccg gaacggactt
     2221 taaaaattcc tgtcctgctc tgaccataca atctgaaaga atttgctttg tttcttctgc
     2281 tttttcttct ttcacttcga ccaggatttc atcgtgaaca gttccgacta tacgagctcc
     2341 tactggcttt atagcttcgt acaaaagacc aagagccatt ttacatatat ctgctgcagt
     2401 gccctggaca ggagtattta accgggcagt taactggaaa tactcttcat tctcagagaa
     2461 aactcttctt cttcctgaaa gtgtcctggt ctccataggc ttttctcttt ttgtcttgtt
     2521 atgccatttt ttcaggccct tataaccatt aaagaatttc gctttaaatt cttccgcctg
     2581 gtcaagtgtc atcgttaccc cgtaactatt tctcgcatac tcaacgaacc gggcggcccc
     2641 cataccgaag ataagaccaa agtttaaagc ctttgcaagc tgcctttctt ccttcgtcac
     2701 ctcttctcta cctgagacca tctgagcagt caatctgtgc aaatctgctc caccggaata
     2761 ggcctctacc atggtctcat ctcctgatat ttctgctgcc acacgaagtt caatctggga
     2821 gtaatcagcg ataacaaatt tattaccgcc atgaggaata aaacaagatc taaaatcttt
     2881 tgtcctgggg atttgctgta aattcggatc actacatgag aaccttcctg catctgaacc
     2941 ggtctgccag taagtagaat gaattctttt agttttcttg tgtatctgtt ccgggagagg
     3001 aacgagaaaa gaactcagag ccttcgacac acttctgtaa tcgagtatct ttgctataag
     3061 cggaatattc tgcaatctgg caagagtttc aaaacttgta tcagaaacag gaaaacctat
     3121 tttatttaac tgttctttaa gctgcgccgg gctgtctaaa ttcacagcat tttctccgaa
     3181 catatcaagg ttttcttctg aagttttccc atgaataaga gataatattt cttttgataa
     3241 actctctttt ttctcctgat attctgcctt taagatgttc catttctcta tatcaagttt
     3301 tatccctgaa agttccattt ctgcgacagg gtacagacaa ttaaattcta tctctgcagt
     3361 ttttaacagg cacatttcgt aaagcttctc acagagtttt aatctgagag gaagtaatat
     3421 ttctgcatcc atcgccgcat attcaatctg ctcctgtgtc agagggtcat tagaccagtt
     3481 tgatttttga ttctctttcg aaaggtctat accgagatat ttttttgaaa gagcatcaag
     3541 agagcatttt tcttttactc ctgaatttat aagctgcgct gcaatcattg tatcaaaaag
     3601 tttccctctg actggaagta aatgagtcct taaaaatttc aaatcaaatt tagcgttttg
     3661 aagacatttt tttatagata catctgaaag tatttcttta accggctccg ggtttacttt
     3721 aaagcagtct actaataata ctggctgccc ttcacagcct atctgaataa gtcttatctt
     3781 acatgtatga gggtcaaggc ctgttgtttc tg
//
LOCUS       DAOQWT010000089         3810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_24754_length_3810_cov_3.390146, whole genome shotgun sequence.
ACCESSION   DAOQWT010000089 DAOQWT010000000
VERSION     DAOQWT010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24754_length_3810_cov_3.390146"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="PKJ95_02625"
     CDS             complement(<1..1194)
                     /locus_tag="PKJ95_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNR65171.1"
                     /translation="MKIKCEINNIPLEVEKGTTVLDAAKKIGIKIPTLCYFEGLQNPG
                     SCRLCVVEVEGAATLLPSCITEVSDGMKIKTNSKRVRGARKILLELLLSSHPWECNTC
                     DRNQDCELQTLADEYGLREVRFNTEKEKLPLDTSTPGIVRDPNKCILCRRCVTACQEI
                     QNVSTLGPRYRGIKTIIAPAEVDLLSDAVCVQCGQCAAVCPVGAITEHSDIDAVWEAL
                     EDPNKYVIVQTAPAIRAALGECFDLPPGTLVKGRMVTALRMLGFDQVFDTNFAADLTI
                     IEEGNELLTRLKKKLVDKDDSVKLPMFTSCSPGWINYMEHFYPELKDNVSTCKSPQQM
                     FGAVAKTYFADKIGRKKEDMVVVSIMPCTAKKFECNRDEMSDSGVQDVDYVLTTRELG
                     KMIKQA"
     regulatory      complement(1463..1597)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     regulatory      2523..2647
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            2897..>3810
                     /locus_tag="PKJ95_02630"
     CDS             2897..>3810
                     /locus_tag="PKJ95_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNR65172.1"
                     /translation="MLKNSVFKTFLTTIFTISLVGALMMGVVADEPLKGEVIVYHAGS
                     LTVPVDNIIKAFNKVYPNVEVKTEAGGSVALARQIAELGKKVDVMMSADYTVIDNLLI
                     PDHAAWNTVFAVNSLVIMYTDSSKYADEITTENWYEVLLRDGVQFGHSEPEQDPCGYR
                     SVLLFQLAEKHYQIKGLNQDIIAHIPQKNIRPKSVELIALLETGALDYAFEYESVALQ
                     HQAMDARFKFVKLPDAINLSSLAYADDYAKATIELSGAEPGTKIITKGEPIVFSLTMP
                     FSGVNRENTIAFLRFLLDPDLGLKILYEA"
BASE COUNT         1158 a          775 c          616 g         1261 t
ORIGIN      
        1 agcctgttta atcattttgc ccaattcccg ggtagtaagg acataatcga catcctgaac
       61 accgctgtca gacatttcat ctcggttaca ttcaaatttt ttagcagtgc agggcataat
      121 agacactacc accatatctt cctttttcct gcctatcttg tcggcaaaat aagtttttgc
      181 taccgctcca aacatctgct gtggagattt acaggtagag acattatctt tcaattcagg
      241 ataaaagtgt tccatatagt taatccagcc gggtgagcag gaagtgaaca taggcagttt
      301 tacactatca tctttgtcta ctaatttttt ctttaatcgg gttaataatt catttccttc
      361 ttctattatg gtgaggtcag ctgcaaaatt ggtatcaaaa acctggtcaa agcctaacat
      421 tctcaatgcg gtaaccatac gtcctttaac cagagtgcct ggtggtaaat cgaagcattc
      481 gcccaatgca gcacgaatag caggggcagt ctgtactatt acatatttgt tgggatcttc
      541 cagagcttcc caaactgcat cgatatcgct atgttctgtt attgctccta ccggacaaac
      601 tgctgcgcat tgtccacatt gtacgcaaac tgcatcagat agtaaatcaa cttcagcggg
      661 ggcaataata gtcttgatgc cgcgatagcg ggggcctaag gttgacacat tttgaatttc
      721 ctggcaggca gtaacacatc tacggcataa aatacattta ttggggtccc tgactatccc
      781 aggagtactg gtgtccaaag gtagtttttc tttctctgta ttgaatctga cttcacggag
      841 accatattcg tcagccaggg tctgcaattc acaatcctga tttctatcac aggtattgca
      901 ttcccaggga tgagagctta agaggagttc taataaaatt tttcttgcac ctctgactct
      961 tttgctgttt gttttaattt tcataccatc actcacttca gttatacagg aaggaagtaa
     1021 agtagccgct ccttctactt ctactacaca taaccggcag gaaccagggt tttgaagccc
     1081 ttcaaagtaa cagagggtgg gtatctttat acctattttt ttagcagcat ccaatacggt
     1141 tgttcctttt tctacttcta atggtatatt gtttatttcg cattttattt tcatatttaa
     1201 ctccgaatat tatttttatg gtatttcata aatttttaag tttacccata tagcaatttc
     1261 taattattgc tccttatata aattttaata atttttaata gttcaaattg tctaaaatat
     1321 ttataataat gctcaaatca tgatgtttct cctacctcat acaaattgat aaaatataaa
     1381 atcaatcatg aaaggccaga attttaagca ttttcaggga taatatagta gggttatata
     1441 aaaggttcaa taaatttgag gtataaaaca catactccct cctttccaaa catgggaggc
     1501 atggatattt ctaaccatac cctcgagatt ttaaaatcta ccggttgtct gccctggata
     1561 attctttagg caaaacaact cgaagagata atgattttta tatttttatt atcaatgcta
     1621 aattaacata aaattaaaaa caaatcaaga aaaatgttag ttgagacctg aactcatatt
     1681 attacaataa gtttcacata atatttgtat ttacattttt aacaagagac aaacactatt
     1741 ttacataatt ttattcgcta ttaccacttc ccctccgtca ttgctagtct accttttctc
     1801 attttgtcat tgcgaacgct gcgttagcag cgtgtggcaa tctcattcaa ttattcccac
     1861 actcttttct ttatattttc tttttattgc tataaattaa aaaccataaa caaataacta
     1921 taaacctctt tcaattattc ccaaaatgat ttatccaaaa tccaaaaact ataaactatc
     1981 aacctcccac acttactccc acatctccgt attggtattt gtatttgtca ttgcgaacgc
     2041 tgcgttagca gcgtgtgtca atctcatcca attattccca catctttgca tttacatttt
     2101 taccaaattt ctcctcgcac tctctgtctt tgcgaggagc atagtgacga agcaatctca
     2161 tactctacag tccgcactct tttcttttca attccatcca catttctccc accgtcttcg
     2221 tatttgcatt attctgtcaa ccaaaaccca tgaactattg accaccctct tttattgcta
     2281 catcttatag ctaaaaacta ttaaccatga accaaaaacc cccctgactt atccccacat
     2341 ctttttcacc aaataccaac tactaaatac caacgaccac ttacatttaa actcatactt
     2401 aatgtattta acaatatgtc ttgactttta atcacatatt ccttaaaata attacggttt
     2461 gttcctcatt tcacaaagat tgagaaagca ccttaaaaaa ttaatatatt aaaaacaatt
     2521 ttttgctcat ttaatttcga ctccatattt ctaaagttaa tatttcaaac tatggatatg
     2581 gagcgatagg gtataccggg aaaccgatat gcctcccatt atggaaagaa attaaagcta
     2641 taaatatatg ttcatgttta cttcaggact tccttaatgg gaagtccttt tttttgtttg
     2701 actaccgtac tatcaggaaa attttaaaga tagcttgaac ttaccaatca tcagaagaat
     2761 aaacatatcc tttctacggg tatgttaatt gatttcctgt taggttattt accagggagg
     2821 agtgatatat taaacaaaaa ttatttccaa aatactttac aagaattgat aataaaaaat
     2881 ggaggactta gaattaatgt taaaaaatag tgtgtttaaa acttttctta ccacaatctt
     2941 taccatatct ttggtgggtg cattgatgat gggtgtagta gctgatgaac ctttaaaggg
     3001 tgaagtgatt gtctaccatg ccggtagtct gactgtcccg gtagacaata ttataaaagc
     3061 ctttaataag gtatacccca atgtcgaagt caaaactgaa gctggaggaa gtgttgccct
     3121 ggccagacag atagccgaac tgggcaaaaa agtagatgtt atgatgtctg cagactatac
     3181 cgtaatagac aatcttctta ttcctgacca tgcagcgtgg aacaccgttt ttgcggtaaa
     3241 ttctctggtg ataatgtata ctgacagcag taaatatgct gatgagatta ctacagaaaa
     3301 ctggtatgaa gttctgctcc gtgatggagt acaatttgga catagtgaac ctgaacagga
     3361 cccctgtggc taccgttctg tattgttatt ccaattagcc gaaaaacatt atcagataaa
     3421 aggattgaat caggatatta tcgctcatat tcctcagaag aatattcgtc ctaaatctgt
     3481 ggagttgatt gccctcttgg agaccggtgc acttgactat gctttcgaat acgaatctgt
     3541 tgccctgcag catcaggcta tggatgctcg tttcaaattt gtcaaattac cggatgctat
     3601 caatctgagc tctctggctt acgctgatga ttatgccaag gcaactatcg aactttcagg
     3661 ggcagaacca ggtaccaaga ttattaccaa aggtgagccc attgtcttca gtttaaccat
     3721 gccctttagc ggggtaaata gagaaaatac tattgccttt ttaagattcc tattagatcc
     3781 cgacctggga ttaaaaattc tttatgaagc
//
LOCUS       DAOQWT010000090         3809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_24764_length_3809_cov_2.880927, whole genome shotgun sequence.
ACCESSION   DAOQWT010000090 DAOQWT010000000
VERSION     DAOQWT010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24764_length_3809_cov_2.880927"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PKJ95_02635"
     CDS             <1..644
                     /locus_tag="PKJ95_02635"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNR65173.1"
                     /translation="TGLAVLFIEVGQEACIINGNYVEAINKGVEAAYQEGYLRKSVVD
                     DPVFDRKNTKTNTPAFIYTDIIPGDRIKITVLPKGFGSENMSGLAMLTAAHGAKGIMD
                     FIVDTVRKAGPNPCPPTIVGVGIGGTADKAMVIAKKATARPIGEYNANPKYAQMEKET
                     LERINKLGIGPAGLGGNTTSLAVHIDYLPTHIAGMPVAVNICCHAARHASGEI"
     gene            678..1238
                     /locus_tag="PKJ95_02640"
     CDS             678..1238
                     /locus_tag="PKJ95_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HNR65174.1"
                     /translation="MSEEIKITTPLDESIVLKLKAGDSVKITGNIYTGRDAAHKRLYE
                     LIEEGKDLPFSLEGQIIYYVGPAPAKPGYACGPAGPTTSYRMDPYTTALLEKGLKGMI
                     GKGLRSKEVIESMKKNKAVYFAAVGGAAALIAKTIKKSEVIAYSDLGAEAIHRYYVED
                     FPAIVCIDAYGNNLYKSEPPKYRKEL"
     gene            1275..2450
                     /locus_tag="PKJ95_02645"
     CDS             1275..2450
                     /locus_tag="PKJ95_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNR65175.1"
                     /translation="MDINQEALKLHSENRGKIEVVSKTKIKDMHDLAVVYTPGVAEPC
                     RKIHQNVSDVYTYTVKGNMVAVISDGTAVLGLGDIGPEAAIPVMEGKAVLFKYFGGVD
                     AFPICLATKDTDEIVETVTRIAPVFGGINLEDISAPRCFEIEKRLKETLDIPVFHDDQ
                     HGTAVVVLSGVINALKLANKKMEEVKMVVNGAGAASIAVAKFLLSAGAQNIILCDSRG
                     IVYEGRTENMNSAKEEMAKLTNKDKVKGNLADALVGADLFLGLSGPNLVTSEMVKRMA
                     HDPIVIAMANPIPEIYPDEARAGGARIIATGRSDFPNQVNNCLGFPAIFKGALKVRAS
                     EINEEMKLAAAHAIASVIPDNELSETNFIPNVFHPEVMEKEAEAVAQAARDTGVARI"
     gene            2802..3245
                     /locus_tag="PKJ95_02650"
     CDS             2802..3245
                     /locus_tag="PKJ95_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNR65176.1"
                     /translation="MKNKFKDFELVEHTADIGLKIYGKDEKDLFINAARGMFFLITGS
                     RISLAPESIRKYYALESEAPGREDLLVTWLNDLLYIHFTDLVIFDDYIIDYISEEVIK
                     SKVRAVEIKDSPYQIVKEIKAVTYHNLQVCQNKNGIWEANIVFDI"
     gene            3331..>3809
                     /locus_tag="PKJ95_02655"
     CDS             3331..>3809
                     /locus_tag="PKJ95_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNR65177.1"
                     /translation="MTIKDNWKNILKQQDTYIWDIPKEFNPGMLVDGRVYASESLLKQ
                     IVTDKSLDQVVNVACLPGIVGYSLAMPDIHWGYGFPIGGVAATRLENGVISPGGVGFD
                     INCGVRLIRSNLKLKDVQERMSQLVEHLYNKIPSGVGSRGIIKLNLAEAKRVLAEGS"
BASE COUNT         1255 a          605 c          879 g         1070 t
ORIGIN      
        1 ataccggtct ggcagtatta ttcatagaag tgggtcagga agcatgtatt attaacggaa
       61 actatgtaga ggcaataaat aaaggagtag aggcagcata ccaagaagga tatctaagaa
      121 aatcagtagt tgatgacccc gtttttgatc ggaaaaatac taaaaccaat accccggctt
      181 ttatctatac tgatattatc cctggagata gaattaaaat aacagttcta cccaagggtt
      241 ttggaagcga gaatatgagt ggactggcta tgctgaccgc tgcgcatggt gctaagggta
      301 taatggattt tattgtggat actgtaagaa aagcagggcc aaacccctgt ccgccaacta
      361 ttgttggagt aggtatcggt ggaactgcgg acaaagcaat ggtaatagcc aaaaaagcta
      421 ctgcccgccc tattggagag tataatgcta atcctaaata tgctcagatg gaaaaggaga
      481 cccttgagag aataaataaa ttggggatag gaccagccgg cttggggggt aataccacat
      541 ccctggcagt acatattgat tatctaccaa cacatattgc aggtatgcca gtggcagtaa
      601 atatatgctg tcatgctgca cgtcatgctt caggagaaat ataatttatt gaatattact
      661 ggaaggagaa ttaattaatg agtgaagaaa ttaagattac tacccctctg gatgaatcta
      721 ttgtattaaa attaaaagca ggtgattcag ttaaaataac gggaaatatc tataccggta
      781 gggatgctgc ccacaagaga ttatatgagt tgattgagga agggaaagac ctgccttttt
      841 cactggaagg tcaaataatt tattatgtag gacctgcgcc cgccaaaccg ggatatgcct
      901 gtggtcctgc aggtcctact accagttacc gtatggaccc ttatactaca gctttattgg
      961 aaaaagggct taaagggatg attggtaaag gattgcgttc taaagaagta attgaaagta
     1021 tgaagaagaa taaggctgta tattttgcag ccgtaggcgg agctgcagct ttaattgcta
     1081 aaactattaa aaaatcagag gtgattgctt attctgacct gggagcagaa gccatacacc
     1141 gctattatgt ggaggatttc ccggctattg tttgtatcga tgcttatggg aacaatttat
     1201 ataaaagtga accgcctaaa tatagaaaag aattataaat aaaatatatt ataaaaatta
     1261 aaaaggagat taaaatggac ataaaccaag aagctttaaa actgcacagc gaaaatagag
     1321 gaaagattga ggtagttagc aagaccaaaa ttaaagatat gcatgatctt gctgtagtct
     1381 atacccccgg agtagctgaa ccttgccgca aaatccatca gaatgtttca gatgtctata
     1441 cctacactgt caagggtaat atggtagcag taatatcaga tggaacagca gtgctgggac
     1501 tgggagatat tggtcctgag gctgccattc cggttatgga aggaaaggca gtgctcttta
     1561 aatattttgg tggtgtcgat gccttcccaa tatgcctggc tactaaagat acggatgaga
     1621 tcgtagagac ggtgacccgg attgctccgg tatttggagg aattaacttg gaggatatta
     1681 gtgctccccg ttgttttgaa atagagaaga gattaaaaga aacgctcgac atcccggtct
     1741 tccatgatga tcagcatgga actgctgtag tagtcctctc cggagttatt aatgctttaa
     1801 aattggccaa caagaagatg gaagaggtta aaatggtggt taacggtgcc ggagctgctt
     1861 ccattgcagt agctaaattc ttactatctg ccggtgctca aaatatcatc ctctgtgatt
     1921 caaggggtat agtatatgag ggacgtactg aaaatatgaa ttcagccaaa gaagaaatgg
     1981 caaagctaac caataaagat aaggtaaagg gcaaccttgc cgatgccctg gtaggtgctg
     2041 atctattttt gggattgtcc ggtcccaatc tggttacctc agagatggtc aaaagaatgg
     2101 ctcatgatcc cattgtaatt gccatggcta atcccatacc tgagatttac cctgatgaag
     2161 ccagagccgg tggtgcccgt atcatcgcta ctggccgttc cgacttcccc aaccaggtca
     2221 acaattgttt gggattccct gctatcttca aaggtgcctt aaaagtgaga gcgagtgaaa
     2281 ttaacgaaga gatgaagctg gcagcagccc atgccattgc ttcggtaatt ccggataatg
     2341 aactcagtga gactaacttc attcccaatg ttttccatcc ggaggtaatg gagaaagaag
     2401 ctgaagcagt ggctcaagca gcaagggata ccggggtagc aaggatatag aattgcagtt
     2461 tgttgacaag ataataaata gaatttataa tatcaaaaag aaataaaata ttaaatagga
     2521 taaaaataaa taaaattttt gagaagaaga gtaaatcctt gtttgacata cagagagccg
     2581 ttaaaggtga aaagacggtt gcaaacctga ttgaagtccg gcttctcttg aaagacaatt
     2641 tgacctggat atttaaaata taattttatg aatattttta ttttgaatag aggatagaat
     2701 tttctattaa attagggtgg caccacgtga ataaactctc gtccctaagg cattgaacaa
     2761 ttgccagaag acgagagttt ttttatttca gggagcaata gatgaagaac aaatttaaag
     2821 attttgaatt agtggaacat acggcagata taggtttgaa aatatatggg aaggacgaaa
     2881 aagatttatt tatcaatgca gctcgaggaa tgtttttttt gattactggt tctcgtattt
     2941 ctctagctcc ggaaagtata cgaaaatact atgcattaga aagtgaagct cctggcagag
     3001 aagatttatt ggttacctgg ttaaatgatt tattatatat tcattttact gatttagtaa
     3061 tctttgatga ttacattatt gattatattt cagaagaagt gattaaatct aaagtcagag
     3121 ctgtagagat taaagattcc ccctatcaga tagttaaaga gataaaagca gttacttacc
     3181 ataatttgca agtttgccag aataaaaacg gaatatggga ggcaaacata gtatttgata
     3241 tttaatgtta aatcttttaa tattcttata gaggaacgat actgattgag aagattctca
     3301 tcatctttac tgtaaaggag ggatgatact gtgactatta aagataattg gaaaaatata
     3361 ttgaaacagc aagatactta tatttgggac ataccaaagg aatttaatcc tggaatgctg
     3421 gtggacgggc gtgtttacgc atctgaaagt cttttgaaac agatagttac tgataaatca
     3481 cttgatcaag tagtcaatgt ggcttgtttg ccaggtattg tgggctattc tttggcaatg
     3541 ccagatattc actggggata tggatttccc atagggggtg tggcagctac ccgtctggaa
     3601 aatggggtta tttcacctgg aggcgtaggc tttgatataa attgtggggt aagactgata
     3661 cgttctaatt taaaacttaa ggatgtacag gagagaatga gtcagctagt tgagcatctt
     3721 tacaataaaa ttccttccgg agttggttcc agaggaatta ttaagttaaa tctggctgag
     3781 gcaaaaagag ttttagcaga aggttcgca
//
LOCUS       DAOQWT010000091         3792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_24903_length_3792_cov_3.038801, whole genome shotgun sequence.
ACCESSION   DAOQWT010000091 DAOQWT010000000
VERSION     DAOQWT010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24903_length_3792_cov_3.038801"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2088
                     /locus_tag="PKJ95_02660"
     CDS             <1..2088
                     /locus_tag="PKJ95_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR65178.1"
                     /translation="VGDNLLIGAVLAMLVLYAFLRSIKSTLIIGLSIPISVISTFTLL
                     YFSKLTLNMMTLGGLSLGVGMLVDNSIVVLENIFLHRELGEEASLAAEMGANEVGEAI
                     TASTLTTIAVFLPIVYVSGIAGELFKTMGLTITFSLLMSLLVALTLIPMLSSKQRKSF
                     HLSGQNTNNVPAGAKKTKKKGFFAFIIQEYSYLIDWSLRHRGIVVIMAILILVVSFSL
                     LSLVGVEFFPAMDQGNFNINISLPVGTNFEVTKGITREIEQVIVHIPEIKDTFTTIGS
                     GGTMGMGSSANNATIMATLLEKDKRERKSSEIIAELRERIGEYPDTKIHFSEQNLSFT
                     SSSPLTINITGDSIEELDFIARNIISILSEVEGVYDLQSSVEEVRSELHIAIDREKVN
                     LYGLSAGQVASNVQDALLGKVAGYFQEGGDRYDITVKLEKSDYDELQELENMIISSSY
                     GLQIPLKEIAEVKVGIGPQGINRENQQRQVTVTGNISGRFLGSVIKDCKEKLDLLVLP
                     DGYLYAFAGEQQEMVKSFRELFYALILSLFLVYMIIAAQFESLLFPFAVILSVPFAFI
                     GVIIALMLTGTSLNVLSLIGLIMLGGIVVNNAIVLIDYINQLRKKGMERNDAVIQAGR
                     TRLRPILMTTLTTVLAMVPMALATGEGAEMRPSLAITIMGGLTSSTFLTLVFIPIFYS
                     YLDDWAKKLHIQF"
     gene            2358..>3792
                     /locus_tag="PKJ95_02665"
     CDS             2358..>3792
                     /locus_tag="PKJ95_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNR65179.1"
                     /translation="MNNKTKIKCFLFFSIFLMLLFLSLSPTVFAQNNGRITAIVVRGN
                     EQIDTKFLVSLISSNIGDLFSKDKVQADMKALFDLGYFQDVQVKLEPFRDGYRVVFQV
                     KENSLIKDIIVEGSTILKEEEIKKVMVLREGQVFSQKILKNDLDRISQLYKDKGLILA
                     QLEEFDFNQNTGALSIKLAEGVIEEIKISGNEKTVDRVIRRELDIEPGELFDFSRIRK
                     SLQDIYNLGFFEDVSMRLEPGSNENLIVVVIEVKEKTTGLLGGGGGYSTGEGLFAYAS
                     VKESNLFGSGQSVEAKLEVGARTTYSFSFYEPWLANTPTFFGLDVYDTYLEVNKKIDG
                     VDSKYEMERLGGKLTFGRNFKEYLKIGLELKTEEATYKLISGKLPSNIQEGVANSFRP
                     IVIYDTRDDRFNPKEGWYGTASIQNAGGILGGDYDFRKYDLDLRTYLSTNIFKEEPKT
                     EETTISGTINQGVLALRAVVGIGSTSLP"
BASE COUNT         1200 a          592 c          796 g         1204 t
ORIGIN      
        1 gtaggtgata atttgcttat tggcgctgta ctggctatgt tagtcctgta tgcctttttg
       61 agaagtatta aaagcacttt aattattgga ctctctatcc ccatttctgt tatctcgact
      121 tttaccctgc tttattttag caaactaact ctgaatatga tgacccttgg cggtctctcc
      181 ttaggagttg gaatgctggt ggacaactca atcgtagttt tggaaaatat attcctgcac
      241 cgggagttag gcgaagaggc gtcactggca gctgaaatgg gcgccaatga ggtaggagaa
      301 gcaattactg cctctacttt aactaccata gccgttttcc tgcccatagt atatgtatca
      361 ggaatagcgg gagaattgtt caagaccatg ggtcttacca tcactttttc tcttctaatg
      421 tcattgttgg ttgccttgac tctgatacct atgctttcct ctaaacagag aaaatctttt
      481 cacttatcag gtcaaaatac caacaatgta ccagcagggg ctaaaaaaac aaaaaagaag
      541 ggattttttg cctttattat acaggaatat agttatctca ttgattggtc tttaaggcat
      601 cggggtattg tggttattat ggctatttta attttagtgg tatccttttc actcctctct
      661 ctggtaggtg ttgaattctt tccggcaatg gatcagggca acttcaatat aaatatatcc
      721 ttacccgtag gaaccaattt tgaagttacc aaggggatta ccagagaaat tgaacaggtt
      781 attgtacata ttccggaaat caaggacacc tttaccacca ttggaagcgg aggaacaatg
      841 ggtatgggtt catcggcaaa taatgctacc ataatggcaa ctttgctgga aaaagacaaa
      901 agagaacgga aaagttcaga aattattgct gaattgcggg aaagaatagg agagtatccc
      961 gatacgaaaa tacattttag tgagcagaat ctctccttta cctcgagctc accactcacc
     1021 atcaatatta ccggagattc tatagaagag ttagatttta tagccagaaa cattatttct
     1081 atattatctg aagtagaagg ggtttatgat ttacaaagca gcgtcgaaga agtacgttcg
     1141 gaattacata ttgccataga ccgggagaag gtaaatcttt acggcttaag cgccggtcag
     1201 gttgcttcaa atgtacagga tgctcttttg ggcaaggtag ccggttactt tcaagagggt
     1261 ggagatcggt atgatataac tgtgaaatta gaaaaaagcg attatgatga acttcaggaa
     1321 ttagagaata tgattataag ttcaagctat ggtctacaaa ttccattaaa agaaattgcc
     1381 gaagtcaagg taggaatagg tcctcaagga attaaccgtg aaaatcaaca gagacaggta
     1441 actgttaccg gaaatataag tggcagattt ttaggtagtg ttattaagga ttgtaaggaa
     1501 aagttggatt tattagtttt acctgatggt tatctctatg cctttgccgg agaacaacag
     1561 gaaatggtaa aatcctttcg cgaacttttc tatgccttaa tattgtcatt atttttagtg
     1621 tatatgatta ttgctgcaca gtttgaatcg ctattattcc cctttgcagt tattctttct
     1681 gttccctttg cctttattgg agtgattata gctttaatgc taacagggac cagtcttaat
     1741 gtcctttctc tcatcggttt aataatgctg ggaggtatcg tggtaaataa tgctattgtc
     1801 ttaatagatt atattaatca attaaggaaa aaaggaatgg agcgtaatga cgcagtaatt
     1861 caggccggaa gaacccgatt aagaccgata ctgatgacta ctcttaccac tgtgctggca
     1921 atggttccta tggctcttgc taccggagaa ggggcagaaa tgagaccatc tctggcaatt
     1981 accattatgg gaggattaac ttcctctact tttctgaccc ttgtttttat tccaatattc
     2041 tattcttatc ttgatgattg ggcaaaaaaa ttacatattc aattttaaac taaataaaaa
     2101 atatttttat aaaattagtt tagatatttt aagatatgtt atataatata aaggataaaa
     2161 tgctattaga cagctaaaat ataaaacctg aagttacctt gacagagaga tgcctgtatc
     2221 ttttttaaga tgttgtacct ttattaattt gtacagattt cgaatattgt ttgtattgag
     2281 aatagatttt attaaattaa gatagaaata ttttgaaaaa taattgagaa gaataaatat
     2341 ccgggaggga aaacaaattg aataataaaa caaaaataaa atgtttttta tttttttcca
     2401 tattcttaat gctgcttttc ctgtcattaa gccctacagt ttttgcccag aataatggta
     2461 gaattaccgc tattgtagta cggggtaatg aacaaattga taccaagttt ttagtttctt
     2521 tgatttcttc caatattggt gacctctttt ctaaagataa ggttcaggct gatatgaagg
     2581 cactctttga tttaggctat tttcaggatg ttcaggttaa attagagccg ttcagagacg
     2641 gttatcgtgt tgtctttcag gttaaagaaa attccctgat aaaagatatt attgtagagg
     2701 gaagcactat attaaaagag gaagaaatta aaaaagttat ggtcttgcga gagggacagg
     2761 tcttttctca aaaaatatta aagaatgacc tggatagaat ttctcaatta tacaaagaca
     2821 aaggtttaat tctagctcaa ctggaggagt ttgattttaa ccagaatacc ggtgcacttt
     2881 caattaagct tgcagaaggg gttatcgaag agataaaaat tagtggtaat gagaagacag
     2941 tagatagagt aataaggcgt gaattggata ttgagcccgg agaattattt gatttcagca
     3001 gaataaggaa atctttacag gacatttata accttgggtt ttttgaagat gtctctatga
     3061 gattagaacc tggcagtaat gaaaatctga ttgtagtggt aattgaagtt aaagaaaaaa
     3121 ctaccggttt attaggaggg ggaggcggtt acagtaccgg cgagggtctc tttgcttatg
     3181 ccagtgtaaa agaatccaac ctttttggaa gtgggcagag tgtagaagca aagctggaag
     3241 tgggtgctag aactacatac agtttttctt tctatgagcc ctggctggca aatactccta
     3301 ctttttttgg tcttgatgtc tatgacacct acctggaagt taataaaaaa attgatggtg
     3361 ttgattcaaa atatgaaatg gaaagattgg gtggtaaact taccttcggg agaaatttta
     3421 aagaatatct aaaaattggc ttagagttaa aaactgagga agccacctat aagctaatat
     3481 ccggtaagct gccctctaat attcaagaag gggtagctaa cagtttcagg cctatagtga
     3541 tttacgatac ccgtgatgac cgctttaatc ctaaagaagg atggtatggc actgcctcta
     3601 ttcaaaatgc cggaggtata ttgggtggag attacgattt tcgaaaatat gatttagatt
     3661 tgcgtacata tctttctacc aatattttta aagaagaacc aaagacagaa gagactacca
     3721 tcagtggtac cataaatcag ggtgttctgg ctttaagagc agtggtagga atcggaagca
     3781 cctctttgcc tt
//
LOCUS       DAOQWT010000092         3786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_24966_length_3786_cov_2.923077, whole genome shotgun sequence.
ACCESSION   DAOQWT010000092 DAOQWT010000000
VERSION     DAOQWT010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24966_length_3786_cov_2.923077"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..1097)
                     /locus_tag="PKJ95_02670"
     CDS             complement(297..1097)
                     /locus_tag="PKJ95_02670"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HNR65180.1"
                     /translation="MNQKNTYQSLGPIETNIISRLTYEKRNIIRAKELDQLFNLSPED
                     RKQIVFRLKKKNILLTIKPGIYVFSPLEAGLQGTGVNEFLIPPIFFPKKNYYIGYSTM
                     YSYYGFTEQLFQTVYVLNTSKYMGKIICGLSYKFIKIPENCFYGLEKIKVKDEEVNIS
                     SKERTLIDLIYFNKPVGGRKRASEILKKFVNNDKCDIVKLVEYATRFPIIKTRKQIGL
                     ILDETKITKKALIPLVKSIEKTSIISFENTRRGTLNKKWRVIINAAQE"
     gene            complement(1345..3075)
                     /locus_tag="PKJ95_02675"
     CDS             complement(1345..3075)
                     /locus_tag="PKJ95_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65181.1"
                     /translation="MMDWMEPADRIIKFTHDILDFCASDPDLFLEMIQERNNFLTKNP
                     EKYNQTEDLREIADLKFEDYYIYFYTSKYYRKIPLKVFLLKMLSQYNPQEQKILKGFQ
                     NNILSSFYVKEAFPGVYFIVEDLISGNQYKIREDQSLFQMKKNDYFIGRILPYETGYA
                     LSCVYLYLQGIPYDAAKKFWGNLPIDFLNKINPLDIEKIFIKQGMESEYVEDNWNLTP
                     RDDFQGKSPEEMVQGSIGPLEKKLSGDFKKYILENMSISKFSDKDTLEKAIKEFQEKW
                     LNKSQEELKGKTHLQVIKEERERLDSPRKDFPILFNIKPLDLGSQNPFNLDDINEEDS
                     PVARDMEIFVSYIQGNRIKVTKKNCWIPFKHLKLIEKNFINPEKDGFLFLGKEEETGK
                     ETRKKYIHFIHLLSRAAKFIYHDKKGYIQVNHKHFKKFTQNNYGEKTNKLLIDWIEKV
                     NWVKLQPTEYTTFNAQDYQENIVNIWVYLYHLKTDKKKTLVPLLKEMYWELSMEQEKF
                     NNIVEDLGPTVQKIILRYLKWFGVIDTIEKLIFPDLGIRIIKRFWITPKGKKLIGRVL
                     MDFWEKGKIQ"
     gene            complement(3134..>3786)
                     /locus_tag="PKJ95_02680"
     CDS             complement(3134..>3786)
                     /locus_tag="PKJ95_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNR65182.1"
                     /translation="KNHLIQGYTINQKRLQESQTKIKELQKTSRMIERLLLQQNLEYT
                     EATGLLQVILDYQKALHLLDEYDHQELQIKDVTTQEKFKLTHEKARKELNKLKGQYTS
                     GLFGLEKDQSFASSIGAIYQSFDGKEVYPSIEEKAAHLLYFVVKNHSFVDGNKRIAAS
                     LFLWFLNENGILYKEDGSKRIADNALVALTLLIAESLSEEKDIMIKVIVNLINQEN"
BASE COUNT         1131 a          634 c          544 g         1477 t
ORIGIN      
        1 aaaagattga aaaggagtaa agacagtacc atttaaaata acaattcttt gttctttatt
       61 actcatcatt tctcattctt ataataaagt gtaaacacaa cttgaaaata gtacaactta
      121 agcgataata agaataataa atcttattaa ggcaagttcc tccgataaat atgagattat
      181 ctgaaaggtt attaatgctc gaaagaatta aagttaagta ataatctttt tctatcaatg
      241 gtaatagaaa tcctttgttt cttgaagctc tttctaatat ctttataaat tcttctttat
      301 tcttgtgcag cattaataat aactctccat tttttattaa gagttcccct acgtgtattt
      361 tcaaaagaaa ttatagatgt tttttctata cttttaacta acggaatcaa tgctttttta
      421 gtaattttag tttcatcgag tatcaatcca atttgttttc tcgttttaat gatgggaaat
      481 ctagtggcat attctactaa ttttacgatg tcacacttat cattgttgac aaattttttc
      541 aatatttctg atgctctctt cctacctcca acaggtttat taaaataaat aaggtctatt
      601 aatgttcttt cttttgagct tatatttact tcttcgtctt tcactttaat tttttctaga
      661 ccataaaaac aattttcagg tattttgata aatttataag ataagccaca aataatcttt
      721 cccatatact tactagtatt caaaacatag acagtttgaa aaagttgttc ggtaaaacca
      781 taataactat acatggtaga gtatccgata taataatttt ttttgggaaa gaaaattggt
      841 gggattagaa actcattaac tcctgtaccc tgcagtcctg cttcaagagg tgaaaacaca
      901 tatatcccag gttttattgt taatagtatg tttttcttct ttaacctgaa tacaatttgc
      961 tttctatctt caggagaaag attaaataat tgatccagct ctttcgctct aataatattt
     1021 ctcttttcat atgtcaagcg agaaataatg tttgtctcta ttggcccaag actctgatat
     1081 gtattttttt gattcatagg ttatttacca aataacttat catacaattt tatacattag
     1141 tataaatgta gtttaacaca tttgtcaaca gcttaaaagt attagctata aaggttggtt
     1201 gagttttatt tccagaggaa ttttctgaga tgtttgttga gctttatttc gattctataa
     1261 tgacttcggg attttttatt ttctatagga acggggattc ttctttcttc cttctagtca
     1321 tttaaaaaaa tcaaatctat ttattcattg gattttgcct ttttcccaga aatccatgag
     1381 cactctaccg attaattttt tacccttagg tgtaatccag aaccgtttga ttattcgaat
     1441 cccaaggtct ggaaagatta atttttctat tgtatcgatc accccgaacc attttaaata
     1501 cctcaatata attttctgta cggtaggacc taaatcttca acaatattat taaatttttc
     1561 ctgttccata gaaagttccc agtacatttc ttttaataaa ggaacaagtg tttttttctt
     1621 atctgttttt aaatgataga ggtataccca aatattaacg atattttcct gataatcctg
     1681 ggcattaaat gtggtatatt cagtaggttg cagtttaacc caattaactt tttctatcca
     1741 gtctatcaac agtttattcg ttttctcgcc ataattattt tgggtgaact ttttaaaatg
     1801 tttatgattg acctggatat accctttttt atcatgatag ataaattttg ctgcccggga
     1861 taacaggtga ataaagtgaa tgtatttctt tcttgtttct ttgcctgttt cttcttcctt
     1921 gcctaaaaat agaaaaccat ctttttccgg gttaataaaa ttcttttcaa ttaatttcag
     1981 gtgtttgaaa ggaatccagc aatttttttt agttacttta atgcggtttc cctgaatata
     2041 gctgacaaaa atctccatat ctctggctac cggagaatct tcctcattga tatcatcaag
     2101 attgaaaggg ttctgtgaac ccagatctag aggtttaatg ttaaataata tcgggaaatc
     2161 tttccgagga ctatccaatc tttctctctc ttcttttatg acctgcagat gagttttccc
     2221 ctttagctct tcctgggatt tattcaacca tttttcctgg aattccttaa tggctttttc
     2281 taaagtatct ttatcagaaa atttgctgat actcatgttt tctaatatat atttcttaaa
     2341 atcacctgat aatttttttt ccaagggacc tatactccct tggaccattt cttcaggaga
     2401 ctttccctgg aagtcatccc ggggagtaag attccaatta tcttctacat attcactttc
     2461 cataccttgt ttaataaaaa tcttttcaat atccaaggga ttaattttat ttaaaaaatc
     2521 aataggtaaa tttccccaga atttttttgc tgcatcatat ggtattccct gcaaatataa
     2581 atagacacag gataaggcat aacctgtttc atatggtaaa attctaccaa taaaataatc
     2641 attctttttc atttgaaaga gggactgatc ttctctaatc ttatattgat tacccgaaat
     2701 aaggtcttct acgatgaagt aaacgccagg aaacgcttct ttaacataaa aactgctaag
     2761 gatattattt tggaatccct taagaatttt ttgttcctgt gggttatatt gagaaagcat
     2821 tttcaataaa aatactttca gtggtatctt tcgataatat tttgaggtgt agaaataaat
     2881 ataataatct tcgaatttca ggtctgctat ttctcttaaa tcttcagtct gattatattt
     2941 ttcaggattt tttgtcagga aattgtttct ttcctgaatc atttccaaaa ataaatcagg
     3001 gtctgatgca caaaagtcaa gtatatcatg ggtaaattta ataattctgt cagctggctc
     3061 catccaatcc atcataatta atgtaccttt ccttttcgag agtgtcatcg taagaagtta
     3121 ttaactgtct tttctaattt tcctggttga tgagatttac gattacttta atcattatgt
     3181 ctttttcttc agataagctt tcagcgataa gtagtgttaa tgctaccagg gcattgtcag
     3241 caattctttt actgccatct tctttatata aaataccatt ttcatttaag aaccatagga
     3301 aaagcgaagc agcaatacgc ttatttccat caacaaagga atggtttttc actacaaagt
     3361 agagtaaatg tgctgctttt tcttcaatgc tgggatatac ttctttcccg tcaaaacttt
     3421 gatagatagc acctatagag cttgcgaaag attgatcttt ttccagacca aataagccgg
     3481 atgtgtattg gccttttaat ttatttaatt cttttcgggc tttctcatgg gtcagcttaa
     3541 acttctcctg agttgttaca tcttttatct gtagttcctg atggtcatat tcatcaagta
     3601 ggtgcagagc tttctgataa tcaagaatga cttgtaataa gccggttgcc tcagtatatt
     3661 ccaggttttg ctgtaaaagc aatctttcta tcattctgga agttttctgt aattccttaa
     3721 ttttggtctg actttcctgt aatctttttt gattgatggt gtatccctga atgagatgat
     3781 ttttca
//
LOCUS       DAOQWT010000093         3727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_25591_length_3727_cov_2.691721, whole genome shotgun sequence.
ACCESSION   DAOQWT010000093 DAOQWT010000000
VERSION     DAOQWT010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25591_length_3727_cov_2.691721"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKJ95_02685"
     CDS             <1..478
                     /locus_tag="PKJ95_02685"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNR65183.1"
                     /translation="DRRHSTGRGLCQRDRGAGCLELAEAVIEATEKDNNFHYLYPLEA
                     SIKEKIEIIATKVYGADGVTYLEQAEKNIKLFESVGFGNLPICMAKTHLSLSHDPALK
                     GRPRNYKIPIRDIRASVGAGFLYPLLGTMMTMPGLPSIPAATKVDIDKDGNTVGLF"
     gene            514..900
                     /gene="gcvH"
                     /locus_tag="PKJ95_02690"
     CDS             514..900
                     /gene="gcvH"
                     /locus_tag="PKJ95_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNR65184.1"
                     /translation="MSKEVKEGLLYSKNDEWLKVENDLGTVGVTDYAQDQLGDIVYVE
                     EIKKGAVLRKGDIITTVESVKAVAEVYAPISGEIVEVNQIIIDEPSKINKEPYGNGWF
                     AKLRISNPDEIKELMTAPDYSEYRKE"
     gene            923..2266
                     /gene="gcvPA"
                     /locus_tag="PKJ95_02695"
     CDS             923..2266
                     /gene="gcvPA"
                     /locus_tag="PKJ95_02695"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNR65185.1"
                     /translation="MRYIPSTEEQQREMLKEIGVSSFEELIASIPEKVRLKRKLNLPP
                     ALSEHELEEKIVHIAQKNADFYNIKPLIGAGAYRHFIPEAAKALMQREEFWTCYTPYQ
                     PELSQGTLQAIFEFQSHISRLTKMEVVIPSIFDGASATAEAALMSIRLTHKEKIIISS
                     LLHPHYRETVKTYLEPHNIKIVELPFQNCLTSVDMLKQALDNNTAAVIIQSPNFLGGI
                     EDIEEVTKITHQDGALLIQVIIEAMSLGLLKAPGNMNVDIVAGSTQSYGLDLHLGGPY
                     NAFLATKKSYIRQLPGRIVGETKDKNGKRAFVMTLRAREQDIRREKATSNICTNHNLN
                     VMAGNIYLSLLGTEGLYQYSLLNFRKAHYLEKELLKSGKFSNTFTYPYFNEFLISYQG
                     DIKELQEKLVDNNFIPPLSIVQFYSEEELKQCLLFAVTEIFSREELNYISNLLSR"
     gene            2278..>3727
                     /gene="gcvPB"
                     /locus_tag="PKJ95_02700"
     CDS             2278..>3727
                     /gene="gcvPB"
                     /locus_tag="PKJ95_02700"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNR65186.1"
                     /translation="MKLIFEKSFPGRIAFSLPSDSFDEIKLEDSLPAKYLEKDKKQLT
                     EVSEVDVIRHFTKLSKFNYGVDNGLYPLGSCTMKYNPRINEKMAGLPNFVYAHPFSFP
                     EMIQGSLEVMYKLNQLLCEITGMDQYTMAPASGAHGELTGVLVMRKYFLDHGEKRHKM
                     LIPDSAHGTNPASAAMAEFEVIEIKSNEKGTVDLGSLSQAMDSETVGLMLTNPNTVGF
                     YDEGIETVQNIIHDKGGLLYYDGANLNASLGISRPGDAGFDVVHLNLHKTFSTPHGGG
                     GPGSGVVGVKSKLIPYLPTPLVDLDQNTGKYFFKEVGKKSIGMVRSFWGNFSVLVKTL
                     VWILSLGSDGLYQCSRASVINANYILAKLREYYKPAYDRYCGHECTLTGREYKKYGIK
                     TLDIAKRLMDYGFHPPTIYFPHFEPYADETIMIEPPESEGKEVLDDFIKTMIVISKEA
                     IENPELLTSAPHRTPISRTDDAMAVRRPILTYD"
BASE COUNT         1222 a          653 c          764 g         1088 t
ORIGIN      
        1 agatcgccgc cacagcacgg gccgggggct ttgccagcgt gatcgtggcg ccggttgcct
       61 tgagctggct gaagctgtga tagaagcaac tgaaaaagat aataatttcc actaccttta
      121 tcccttggag gccagtatca aggagaagat tgaaattatt gctaccaaag tatatggagc
      181 agatggggta acctacctgg agcaggcaga aaagaatata aaactttttg aatcggtagg
      241 ttttggtaac ctgcccatct gtatggctaa aacacacctt tctctttccc atgaccctgc
      301 tctcaagggt agacctagaa attacaagat accaataaga gatattagag cctcagtggg
      361 ggcaggtttc ttatacccac tattgggaac aatgatgact atgcctggtt taccatcaat
      421 tcctgctgct accaaagttg atattgataa agatggtaat actgttggat tgttttaaat
      481 aaaaaaatat aaaaatctgg aggtaaaatt actatgtcta aagaagttaa ggaaggatta
      541 ttgtacagca aaaatgacga atggttaaaa gtagaaaatg atcttggaac agttggtgta
      601 actgattatg cccaagacca attgggtgac attgtctatg tagaagaaat taaaaaagga
      661 gcagtgctca gaaaagggga tataattacc accgtggaat cagtcaaagc cgtagctgag
      721 gtttatgcac caataagtgg cgaaattgtc gaagtaaatc aaattataat tgatgaacct
      781 tcaaagataa ataaagagcc ttatggaaat ggatggttcg ctaaacttag aataagtaac
      841 cctgacgaaa taaaagagct tatgaccgct cctgattata gtgaataccg aaaagaataa
      901 attatagaaa gaggtgatta aaatgagata tattccttca actgaagaac agcagaggga
      961 aatgctcaaa gagattggtg tatcttcttt tgaagaattg attgccagca ttcctgaaaa
     1021 agttagatta aagagaaaat tgaatctccc ccctgccctt tctgagcatg agttagaaga
     1081 gaaaattgta catattgctc aaaaaaatgc tgatttctat aatataaaac ctctgattgg
     1141 agcaggtgct taccgacatt ttattcctga agcggcgaaa gcccttatgc agagagaaga
     1201 attttggacc tgttataccc cctatcaacc cgagctcagc cagggaacct tgcaggccat
     1261 tttcgaattt cagagtcata tttctcgttt aaccaaaatg gaagtagtta ttccttctat
     1321 tttcgatgga gcttcagcta cagccgaagc tgcattgatg tccattagat taactcacaa
     1381 ggaaaaaatt ataatttctt ctcttcttca tcctcattat cgagaaacgg ttaagaccta
     1441 tttagagcct cataacataa aaatagtaga attacctttt caaaattgcc tgacatctgt
     1501 tgatatgctt aaacaggctt tagataataa tacggctgct gtaataatcc agagccccaa
     1561 tttcttggga ggaattgaag atattgaaga ggttacaaaa ataactcatc aagatggagc
     1621 cttgcttatt caggtaataa tagaagctat gtccttaggc cttttaaaag ctccggggaa
     1681 tatgaatgtt gatattgttg cgggtagtac ccaatcttat ggtttagatt tacatttagg
     1741 cggaccttac aatgcatttt tagccacaaa aaaaagttat atcagacaat taccgggaag
     1801 aattgtgggt gaaactaagg ataaaaatgg caaaagagct tttgttatga ccttgagagc
     1861 gcgcgaacag gatatcagga gagagaaagc tacttctaac atctgtacca accacaactt
     1921 gaacgtaatg gctggcaata tatacctttc ccttttaggt acagaaggct tataccaata
     1981 ttcacttctc aattttagaa aggcacacta cttagagaaa gaattattaa aatccgggaa
     2041 atttagcaat acttttacct atccttattt taatgaattc ttaatcagtt atcaggggga
     2101 tattaaagaa ttacaagaga aactggtaga taacaacttt ataccgccac tctcaatagt
     2161 gcagttttat tcagaagaag aattaaaaca atgtctgctc tttgctgtta ccgagatatt
     2221 ttccagggaa gaacttaact atatttctaa tcttttgtca cgataaggag gtgcaaaatg
     2281 aaattaattt ttgaaaagag ctttcccggt agaattgctt tttctctacc ttcagattct
     2341 tttgatgaaa taaaattaga agattctttg ccagcaaaat atcttgaaaa agataaaaag
     2401 caactgacag aggtttctga agtagatgta atcaggcatt ttactaaact ttctaaattt
     2461 aattatgggg ttgataatgg attatatccc ctgggttcct gtactatgaa atataaccca
     2521 agaattaatg aaaagatggc tggcttgcct aattttgtgt acgcacatcc tttctctttt
     2581 cccgaaatga ttcaaggtag tttggaagta atgtataaac tcaatcaatt gttatgtgaa
     2641 ataactggga tggaccaata tacaatggct cctgcttcag gtgcacatgg tgaattaaca
     2701 ggtgtcctgg ttatgcggaa atatttctta gaccatggtg aaaaaaggca taaaatgtta
     2761 attcctgact ctgcgcatgg gaccaacccg gcatcggctg caatggcaga attcgaagtt
     2821 attgaaatca agtccaatga aaagggaact gtggacctgg gttcactgtc tcaggctatg
     2881 gatagtgaaa ctgtgggttt aatgttgacc aatcccaata cagttggctt ttatgatgaa
     2941 ggaattgaga ctgtacagaa cattattcat gataagggtg gtttacttta ttatgatggg
     3001 gcaaatctca atgcttcttt aggtataagt cgtccgggag atgcaggctt tgatgtggta
     3061 catcttaatt tacataagac attttccaca ccccatggtg gtggtggtcc aggttcaggc
     3121 gtagtcggag taaaaagcaa actaatcccc tatctgccga ctcccctagt tgatttggat
     3181 caaaacaccg gtaagtactt ttttaaggaa gtaggtaaaa agagtattgg tatggttcgt
     3241 agcttttggg gcaacttctc tgttttagtt aaaaccttag tctggatact ctctcttggt
     3301 tcagatggcc tatatcaatg ttccagagca agtgtcatta atgccaacta tattttggcc
     3361 aagctcaggg aatattacaa acctgcctac gatagatatt gtggccatga gtgtactcta
     3421 accggacggg aatataaaaa atatggaatt aagacccttg atattgccaa aagattaatg
     3481 gattacggtt ttcaccctcc gaccatttat tttcctcatt ttgaaccata cgccgacgaa
     3541 accattatga ttgaacctcc tgagtccgaa ggaaaagagg ttttagatga tttcataaag
     3601 actatgattg tcatctctaa agaagcgata gaaaatccgg aacttttaac ttctgcacca
     3661 catcgaacgc ctataagcag aactgatgat gctatggcag tcaggagacc tattttaacc
     3721 tatgatg
//
LOCUS       DAOQWT010000094         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_25848_length_3701_cov_3.062534, whole genome shotgun sequence.
ACCESSION   DAOQWT010000094 DAOQWT010000000
VERSION     DAOQWT010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25848_length_3701_cov_3.062534"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /gene="glnA"
                     /locus_tag="PKJ95_02705"
     CDS             <1..1222
                     /gene="glnA"
                     /locus_tag="PKJ95_02705"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNR65187.1"
                     /translation="FVRLKFTDIMGIPKNVEVPIRDFKEALDGNIMFDGSTVQGFCRI
                     EESDMFLQPDLDTFMVNPWEEDQYVARTNLKRVIAEWNKTGYTANFGPEMEFYIFERD
                     EKNKPTTRTNDQASYFDLSPIDLGEELRRDMVITLEKLGFEVNTSHHEVGPGQHEIDF
                     KYSDGITTADRIMVLKLTSKTIAMWHNLHVTFMPKPVFGIPGSGMHTNISLFKDGENA
                     FYDPESKYCLSKEAIYFIGGIMKHAKGLAAITNPLVNSYKRLTSGYEAPVHIAWSHCN
                     RSPLIRIPTVRNGNAARIEYRCPDPSCNPYLSFAVILTAGMDGIQKKIMPPDPINENI
                     YDMDEIQLKKYGIESLPTNLKEALEEFKKDKVLHQGLTDHIIDNYLRAKYREWEIYNL
                     QVHNWELEQYLANY"
     gene            1536..3290
                     /locus_tag="PKJ95_02710"
     CDS             1536..3290
                     /locus_tag="PKJ95_02710"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNR65188.1"
                     /translation="MTSAKRIEQNKNHLNEINVFPVADGDTGTNLAVTMNNIVEEVQD
                     FRQNSFDLTMQRIAKSALKGARGNSGAILAQFFQGLAEATRGMSRLTTVAFSQAALKA
                     AEQANMAIAGPCEGTIITVMKDWANHINKLAHKTPDFVELFKNSLARAKKSLLETPEK
                     LAILKQAGVVDAGAEGFVNLLEGIVDFIEFGRLRVMKQESKGIQINSQRQSFFQSLEK
                     SKFRYCTECLLEGVDLDRNLIREKLTNMGDSQVVAGSEEQLRIHIHTDHPEAIFSELD
                     SFGTIVETKIDDMWEQTEKVKRKTILEKIALVADSTCDLPQELIEKYGIKIVPIAVQV
                     DEQSFLDRVNISTKEVVDFLENSAHRVSTSQPPYQYFEDIYQELGEDNEAIISIHLPG
                     KLSGTFQAACTASKQNKYHDKIQVIDAKTSTVGLGMIVLQAARLIENGITLNELLEKL
                     NYYIEHSKLFVSIPTLKYLIKSGRLDKIKGFLGSLMHLKPIISLNQEGEFEEAAKVVG
                     LRRLWDKTLELACQFASGKKNPHFAIAHIEDIDLAKWYEQGIKKHFPEAEIFISEGSP
                     ALSVHIGRGGAAIAVMGG"
     gene            3345..>3701
                     /locus_tag="PKJ95_02715"
     CDS             3345..>3701
                     /locus_tag="PKJ95_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65189.1"
                     /translation="MKIGCYHLISEMMEKRNIDPSLQPFLTSIEKHLGEDLSQISPAE
                     VKKGGILPVIFIKSGGVEEKFKRIYQKFSPPYLLLVSCLFNSLPAALEIKSYLQRRGE
                     KVEVLHGSEVRIAQRLK"
BASE COUNT         1232 a          584 c          803 g         1082 t
ORIGIN      
        1 atttgtacgc cttaagttta cagatataat gggtattcct aagaatgtag aggtgcccat
       61 aagagacttt aaagaggcct tagatggaaa tataatgttt gatggttcaa cggtacaggg
      121 tttctgtagg attgaggagt ctgacatgtt cctgcaacct gatttagata cttttatggt
      181 caacccctgg gaagaggatc aatatgtagc cagaactaat ctgaaaagag tgatagcgga
      241 atggaacaaa accggttata cagccaattt tggaccggaa atggaatttt atatttttga
      301 acgggatgaa aaaaataaac caactaccag aactaatgat caggctagtt attttgatct
      361 ctcacccata gatttagggg aagagctgcg cagggacatg gtcatcacct tagagaagct
      421 gggctttgaa gtaaatactt ctcatcatga ggtaggtccc ggtcagcatg aaatagattt
      481 taaatattct gatggtatta ctactgctga ccggataatg gtcctgaaat tgaccagtaa
      541 gactattgca atgtggcata atctgcacgt gacctttatg cctaaaccgg tatttggtat
      601 tcccggttct ggaatgcata ccaatatctc gctctttaaa gatggggaaa atgcttttta
      661 tgaccctgaa agtaaatatt gtttaagcaa agaggcaatt tattttattg gtgggataat
      721 gaaacatgcc aaaggattag ctgccattac caaccccctg gtgaattctt ataaaaggct
      781 aacctccggg tacgaagcac cggttcatat tgcctggtcg cattgtaacc gcagcccctt
      841 aataaggata ccaactgtga gaaacggaaa tgctgccaga atagaatatc gttgccctga
      901 cccgtcctgt aatccttatc tttcttttgc agtaatcctg actgcaggta tggatggaat
      961 tcaaaaaaag ataatgcctc ctgaccctat taatgagaat atatatgata tggatgaaat
     1021 acaattaaaa aaatatggta ttgaaagctt accaaccaat ctgaaagaag cattagaaga
     1081 atttaaaaaa gacaaggtat tgcatcaggg gctaactgac cacattattg ataattactt
     1141 aagagctaaa tatagggaat gggagatata caaccttcaa gtccataact gggagttaga
     1201 acaatacctt gctaattact aatttagtta gaatatgtgt aagaataggg tttttagtta
     1261 atagtttata gtttttggat tttagttaaa tgcaaaaaca aaaaagtttg tgaaaaagtg
     1321 taagagtaga gttcacagtt tttagtttta ggaaaagtaa aaaagagtgt gaggagaggt
     1381 gtgggaatag atagaaaaag agtgtgggta acagtgaatg agattgccag gaaatgtctt
     1441 caaaagactg gtataatatt taaaatatta ggataaattt ggttatgaaa atacaaataa
     1501 agtacataaa cggaattcgt tttaaaaggt ttataatgac ctctgctaag cggattgagc
     1561 agaacaaaaa ccatcttaac gagattaatg tttttccagt tgctgatggg gataccggta
     1621 ctaatctggc cgttactatg aataacattg tagaagaggt tcaggatttt cgtcagaatt
     1681 cctttgattt aaccatgcag aggatagcta aatcagctct caaaggtgcc aggggaaatt
     1741 caggggctat tttagctcag ttttttcaag ggttagctga ggccacccgg ggaatgagcc
     1801 ggctcactac agtggctttt tctcaggcag ctcttaaggc agcagagcag gccaatatgg
     1861 ctattgctgg accctgcgaa ggaaccatca ttactgtaat gaaagactgg gctaaccata
     1921 taaataagct ggcacataaa acacccgatt ttgtagaatt gttcaaaaac tccctggcaa
     1981 gagcaaaaaa atcattatta gagacccctg aaaaactggc tatcttaaag caagcaggtg
     2041 ttgttgatgc cggggcagaa gggtttgtta atttgctgga aggaattgtt gattttatag
     2101 aatttggcag attaagggta atgaaacagg agtccaaagg aatacagatt aattcacaaa
     2161 gacagtcttt ttttcagtcc ctggaaaaat ccaaattccg ttattgtact gaatgtttac
     2221 ttgagggagt agatttagat agaaatctta tcagagaaaa attaactaac atgggtgatt
     2281 ctcaggtagt agccggttca gaagaacagt taagaataca cattcacacc gatcaccctg
     2341 aagctatttt ttctgagctt gattcctttg gtactatcgt agaaaccaaa atagatgata
     2401 tgtgggagca aacagaaaag gttaagagaa aaactatatt agagaagatt gctctggtag
     2461 ctgattcaac ctgtgattta cctcaagaat taattgaaaa atatggtatt aaaattgttc
     2521 ctattgccgt tcaggttgat gaacaaagtt ttttagaccg ggtaaatatt tcaactaaag
     2581 aagtggtgga ttttttggaa aactctgcac accgggtaag cacctctcaa cctccctatc
     2641 aatattttga agatatatat caggaactgg gagaagataa tgaggccata atttctattc
     2701 atcttcccgg aaagttgagc ggtacctttc aagctgcctg cactgccagt aagcaaaata
     2761 aatatcatga taaaattcaa gttattgatg ctaagaccag tacggttggc ctgggaatga
     2821 ttgtattaca ggctgcccgt ctgattgaga atggtataac gttaaatgaa ttactggaaa
     2881 aattaaatta ttatatagag catagtaagc tatttgtaag tattccaacc ttgaaatatt
     2941 taattaaaag cggacgtctg gataaaataa aggggttttt aggctcattg atgcacttaa
     3001 aaccgataat ctctttaaat caggagggtg aatttgaaga agctgccaaa gtggttggtc
     3061 tacgccgttt atgggataag actttagaac tggcctgtca atttgcatca ggtaaaaaga
     3121 acccccattt tgctattgcc catatagagg atattgattt agcgaaatgg tatgagcagg
     3181 gaataaagaa gcattttcct gaggctgaaa tctttatttc agaaggttct cctgctctca
     3241 gtgtacatat agggagaggt ggcgccgcta ttgcggtgat gggtggttaa aaaatatctt
     3301 tttaacttcc aagaaatgta atattttatt agaggtgaat aacattgaag attggttgtt
     3361 atcatctaat atccgaaatg atggaaaaac gaaatattga cccatccttg cagccctttt
     3421 taacctcgat cgaaaaacac cttggagaag atttatcaca aattagccct gctgaggtaa
     3481 aaaaaggtgg cattttaccg gttatcttta ttaagtcagg cggtgttgaa gaaaagttta
     3541 aaaggatcta tcaaaaattt tctcctcctt atctgcttct agtcagttgt ttatttaatt
     3601 ctttacctgc agctctggaa attaaatcct atttacagag aaggggagaa aaggtggaag
     3661 ttttacatgg ttcggaagta aggattgctc aaaggctgaa a
//
LOCUS       DAOQWT010000095         3700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_25858_length_3700_cov_2.790123, whole genome shotgun sequence.
ACCESSION   DAOQWT010000095 DAOQWT010000000
VERSION     DAOQWT010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25858_length_3700_cov_2.790123"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..738)
                     /locus_tag="PKJ95_02720"
     CDS             complement(22..738)
                     /locus_tag="PKJ95_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65190.1"
                     /translation="MSETILEVDNINCAYDNVPVIFGVSFEVKEKELVAIVGANGAGK
                     STIMKTIAGLVHPKKGAIKYKGKDISHLPANMILRRGISYIPEGRRLFAKLTVVENLE
                     LGAYIENDRGEIEKRKKEILKLFPILEERRKQIAETLSGGEQQMLSIARGLMSKPELL
                     MLDELSLGLMPSLVESVMETIVRIKQQGTTILMVEQKVQEALEIANRGYVIQTGKIVQ
                     AGSSQELLNSDEVKKAYLGM"
     gene            complement(742..1497)
                     /locus_tag="PKJ95_02725"
     CDS             complement(742..1497)
                     /locus_tag="PKJ95_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65191.1"
                     /translation="MPLLEVCEISKKFGSLVANSKISFKVEEGNIVGLIGPNGAGKTT
                     LFNCITGIYKPSEGKVFFDGSDITNLPPYKVARLGAVRTFQIVHSLKEMSVFDNVLIG
                     AYLRERNTEKAREMANQCIDICYLTKIKNTRAGDLAIGHKKRLELARALATSPKLLML
                     DECMAGLTSTEVNESVSLIKSLKKKGITFLIVEHIMEAIMPIADKVVVLNGGLKIAED
                     HPEVVVHQESVIEAYLGQKYSKKFQLAAKGRNI"
     gene            complement(1512..2528)
                     /locus_tag="PKJ95_02730"
     CDS             complement(1512..2528)
                     /locus_tag="PKJ95_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR65192.1"
                     /translation="MIKENTKKQDLLWYVLIVVLLLVPFIPQVAANSFTQHVFILILL
                     YASMSQAWNIIGGYCGQVSFGHSVFFGIGAYGAGMAVVKFGITPWPGIIIGSLLAMVV
                     AVLISYPCFKLKGHYFAIATFAIVEIFNRLFMVWYWVNGALGLDYPMVKTGWTNFVWY
                     RTKIPYYYGALILFTTIFSIVRFIERNRFGYYLRAVREDQETAEATGINSTFIKLSAM
                     ALSAFLASFCGAFFVQYNFRVDPPMVMSLQVSMVFVLVTILGGAGTLFGPLLGALILI
                     PLQEYTRVLWGGLGRGTDLMLFGFLIVLIMVRQPKGIMGLINEYLNYRKTRRIISAQP
                     EINS"
     gene            complement(2532..3392)
                     /locus_tag="PKJ95_02735"
     CDS             complement(2532..3392)
                     /locus_tag="PKJ95_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR65193.1"
                     /translation="MTMFFQVAIDGLLTGMVYALVASGLSLIWGVMDVLNFAHGEFLM
                     LGMYVSYWLGFLINFDPLISWIASGVFCFLLGILTYKLIIHHCLLGPPLSALLATFGL
                     SMILKNFALNRFSPNYRLLTGTLLDGKRTLIGNIVLPMPQLITSIFALVVVFIIYYVI
                     KRTRLGWAIQATAMDKEAAELMGINTERIFLLVFGLGGLCVGVAGGLLPSYLAVHPEV
                     GGLFGLIAFVCVAMGGFGSIPGALLAALLIGLVEALGGFYIAPVFKYVVVFGLYLLVV
                     MLKPKGLFGW"
     gene            complement(3505..>3700)
                     /locus_tag="PKJ95_02740"
     CDS             complement(3505..>3700)
                     /locus_tag="PKJ95_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HNR65194.1"
                     /translation="DTLYANEWDSPLSMGGKVAFVPGGQNAKAMSMITQLQGGEYKRV
                     FPPELSTDEVIFPMVPWSKR"
BASE COUNT         1203 a          868 c          558 g         1071 t
ORIGIN      
        1 aacaaatgat ttattctatt tttacattcc cagatatgct tttttcactt catcagagtt
       61 gagcagttcc tgagagctac ctgcctgaac aattttcccg gtttgtatta cgtatccgcg
      121 gttagcaatc tccaaagctt cttgtacttt ctgttctacc atcaatatag ttgtcccctg
      181 ctgctttatt ctgacaatgg tctccataac actttctacc aaagatggca tcagtcctaa
      241 agaaagttca tccagcatta ataactcagg cttggacatc aaacctctgg caatggaaag
      301 catctgctgt tcgccaccac ttaaggtttc agcaatctgt ttccttctct cttccaatat
      361 gggaaagagt ttcaaaattt ccttttttct cttttcaatt tctccccggt cattttcaat
      421 atatgctccc aattcaagat tttctactac ggttaatttg gcaaataatc ttcgcccctc
      481 aggtatataa ctgatacctc tacgaagaat catattagcc ggtaaatgag atatatcttt
      541 acctttatat ttaatggcac cttttttggg atgtactaaa ccggcaatag tcttcataat
      601 ggtacttttt ccagcaccgt tagcccccac aatagcaacc agctcttttt ctttaacttc
      661 aaaagataca ccaaatataa ccggtacatt atcataggca caattaatat tatcaacttc
      721 taatatagtt tcagacatac tttatatatt cctccccttg gcagcaagct gaaatttttt
      781 actatatttt tgacccaaat aagcttcgat aacactctcc tgatgtacca ctacttctgg
      841 atgatcttct gctatcttta atcctccatt aagtaccacc accttgtcag caataggcat
      901 aatcgcctcc ataatatgtt caactatcag aaaagtaatc ccttttttct ttaaactctt
      961 tattaaactc acagattcat tcacttcggt agaagtaaga cctgccatac actcatctaa
     1021 cattaataat tttggactgg tagccagggc tctggccagc tccagtcttt ttttatgccc
     1081 tatagctaaa tcgcccgctc tggtattttt tattttagtc aaataacaaa tatctataca
     1141 ctgattggcc atttctcttg ctttttctgt atttctttcc ctcagatatg ccccgattaa
     1201 cacattatca aagacagaca tctctttaag ggaatggaca atctgaaatg tacgcaccgc
     1261 accaaggcga gctactttat aggggggtaa attagtaata tctgaaccgt caaaaaacac
     1321 tttcccctcg ctgggcttat atataccggt gatgcagtta aacagcgtgg tcttacctgc
     1381 tccattcgga ccaatcaaac ctacaatatt tccctcttct accttgaaag atatcttgct
     1441 atttgctacc agagagccaa attttttact tatttcacaa acttctaata agggcaatat
     1501 atattacctc cctaagaatt aatctctggt tgagcagaaa tgattcttct agtctttcta
     1561 taatttaaat attcgttaat aagtcccata atgcccttgg gttgcctcac cataatcaaa
     1621 acgattagaa aaccaaataa cataagatcg gttcctcttc ccagacctcc ccataatacc
     1681 cgcgtatatt cttgaagagg aatcaaaatc agggcaccca atagaggtcc aaacaaagta
     1741 ccagcaccgc ccaggatggt aaccaggaca aaaaccattg acacctgaag tgacatcacc
     1801 attggtgggt ctacccgaaa attatattgt acaaaaaagg ctccacaaaa ggatgctaaa
     1861 aaggctgaga gtgccatggc agataatttt ataaaggtac tgttgatgcc tgttgcctcg
     1921 gctgtctcct ggtcttctct caccgctctc aaataatatc caaagcgatt cctttcaata
     1981 aaacgtacaa tactaaaaat agttgtaaag agtattaagg ccccataata ataaggaatt
     2041 tttgttctat accatacaaa atttgtccat ccagtcttta ccataggata atccaaaccc
     2101 agagcaccgt taacccaata ccagaccata aaaagtcggt taaaaatctc tacaattgca
     2161 aaggtagcaa tagcaaaata atggcctttc aatttaaaac agggataact gataagaact
     2221 gctacaacca tcgctaataa cgaaccgatg ataattccag gccagggagt aattccaaat
     2281 ttaaccacgg ccattcccgc cccatatgcc cctatgccaa aaaagacgga atgcccgaag
     2341 gaaacctgtc cacaatagcc accaataata ttccaggcct gtgacatgct ggcatataat
     2401 agaatcagaa taaaaacatg ttgagtaaag ctattagcag caacctgcgg aataaagggt
     2461 actagaagta gtaccacaat caaaacatac cataacaaat cctgtttttt agtattctct
     2521 tttatcattt tctaccaccc aaataatcct tttggtttta acatcactac caggagatat
     2581 agtccaaaaa ctactacata tttaaatacc ggtgcaatat aaaatccccc taatgcttct
     2641 accaaaccta tcaaaagagc agccagtaaa gcacctggta tgcttccaaa tccccccatt
     2701 gctacgcaga caaaagcaat taatccaaac aaaccgccta cttcaggatg aacggccaga
     2761 taggatggca acaatccacc agcaacgcct acacaaagcc cacccaaacc aaagaccaac
     2821 aaaaagattc tttctgtatt tatacccatt aactcagcag cctctttgtc cattgcagta
     2881 gcctgaatag cccaacccag ccgggtacgc ttaattacat aatagataat aaagactacc
     2941 actaaagcaa aaatgctggt tatcaattga ggcattggta aaactatatt gcctatcagg
     3001 gttcttttac catcaagaag agtaccggtt aataaacgat aattgggaga aaatctgttt
     3061 aaagcaaaat tttttaatat cattgataac ccaaaggttg ccagtagtgc agataaggga
     3121 ggacctaaaa gacagtgatg aataattaat ttgtaggtta aaatgcccag aagaaaacag
     3181 aatacccctg aggctatcca ggagataagg ggatcaaaat ttattaaaaa tcccagccaa
     3241 tagctaacat acatccccag catcagaaac tctccatgag caaaattgag tacatccatt
     3301 accccccaga ttaaactcaa cccggaggca accagagcat aaaccattcc agtcaataat
     3361 ccatcaatag ctacttgaaa aaacatagtc ataaagtatt cccttcttaa ctaatcacat
     3421 catggtagta agtattttca tacttactac catgatagtt aggttatttc gtatgttttt
     3481 ttaattttcc tgtttattac tatgttaccg tttactccaa ggaaccatgg ggaatataac
     3541 ctcatcagta gataactcag gaggaaacac tcgtttgtat tctccgcctt gcagctgagt
     3601 tatcatactc atggctttag cattctgtcc accgggaaca aaagccactt tgcctcccat
     3661 agacagagga gaatcccatt cattggcata cagggtatca
//
LOCUS       DAOQWT010000096         3697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_25888_length_3697_cov_2.507414, whole genome shotgun sequence.
ACCESSION   DAOQWT010000096 DAOQWT010000000
VERSION     DAOQWT010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25888_length_3697_cov_2.507414"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKJ95_02745"
     CDS             complement(<1..506)
                     /locus_tag="PKJ95_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNR65195.1"
                     /translation="MKIRKTGIIVNEEKDKNLRVTKKIIGTLNEKNIEVYLPENIIFQ
                     INESKKIHLLSEPYQDLDMFFSLGGDGTMLKAARLASPYKIPVCGVNLGGLGFLTQIG
                     QEEIDIYLPYILNNNYQIEERMMLSGFIEREGQKKEKFYCLNDVVVAKKLFARLIDLD
                     MYINEEYA"
     gene            complement(522..1058)
                     /locus_tag="PKJ95_02750"
     CDS             complement(522..1058)
                     /locus_tag="PKJ95_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65196.1"
                     /translation="MIKNKELYLFIVMLLLGGILSLVLYLKIKNSLSSKKATRRAVIS
                     KRAEKKAEELLKKNGFQIIDRQISKPLIIKESAVLHRYLIRIDLLVKKNGRTYVVEVK
                     SGSKNKITKRETRRQLLEYFLAYQPYGIMLFDMDTKKFSEIRFQLPYFHFKLVENIIF
                     FLLGMLISLLGMYVFVLK"
     gene            complement(1113..1946)
                     /locus_tag="PKJ95_02755"
     CDS             complement(1113..1946)
                     /locus_tag="PKJ95_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNR65197.1"
                     /translation="MMIKKERLDKLLVDMGLTDSREKAKRYIMANLVKVNGIIINKAG
                     TRCNIESKINIEKDNYPFVSRGGFKLKKALTEFEVDVNGKIVIDVGASTGGFTDCLLK
                     YGAEEVYCVDVGYGQLDWALRQDDRVTNLEKTNIRYLQKNRFNFLFDLATVDVSFISL
                     EKVLPVLAELLKDNGEVIALVKPQFEAGRVLVKKGGIVDDPEVHKEVISKLLEKIKNQ
                     FHLLNMTFSPIKNSPGNIEYFLYLEKKIGKDNLSDANINLLKKIVDEAHHYFFAKETH
                     V"
     gene            complement(1972..2574)
                     /locus_tag="PKJ95_02760"
     CDS             complement(1972..2574)
                     /locus_tag="PKJ95_02760"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNR65198.1"
                     /translation="MARRRKKKNSFKSIIDFAVLVILLVIIIFLMQKFIIPLILEKRV
                     PKIEETKEEIELTPPSDVVGEEQIVLYFADGNAQYLVPEYRKIKIVEGIAKQAIIELI
                     KGPSSSELYPTIPSTTRVNGLYIGDGIAYIDLSSEVIKNHPGGSTGELLTVYSIVMTL
                     TSFPEINKVQILVDGNSGETLVGHVDTSIPLERDESWLKK"
     gene            complement(2588..>3697)
                     /locus_tag="PKJ95_02765"
     CDS             complement(2588..>3697)
                     /locus_tag="PKJ95_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNR65199.1"
                     /translation="ASKEIIITSHKPQVTSIRSFSHPDKTRIVIDISEKSSYHVLTLK
                     EPERLVVDIDATLKQLNKKEEELNVDDLLVKRVRSGQFTPEKVRIVADLKGKYEFEVF
                     NLSSPERIVVDIFAPLSQMDIVSQTEIQSETLTEGVQKNTDYVIVLDPGHGGNQPGAI
                     GPGGLKEKDIVLDIALRAKKLLQENGFKVYLTREKDVDVPLEKRPLIAMEKEATVFIS
                     IHTNSAIQKGSPTAKGIETYFLNSKYASDASAKDVADRENRASQFYHQETDLLNKILY
                     DLGESVNIRFSTEFAKIVQGNIVRNTGLTNRGVKQAPFIVLKGVNMAAVLIEVGFISN
                     PNEEKLLKTPEFREKVAQALTRSIKDYVKNIPDNI"
BASE COUNT         1130 a          733 c          492 g         1342 t
ORIGIN      
        1 attgcatact cttcatttat gtacatatct aaatcaatca atctggcaaa taattttttt
       61 gcaactacaa catcattgag acaatagaat ttttctttct tttgaccttc tctctcaata
      121 aatcccgata acatcattct ttcctcaatt tgataattat tgtttaaaat ataaggaaga
      181 tagatatcta tttcttcttg accaatctga gttaaaaaac ccagaccgcc taaatttact
      241 ccacagaccg gaattttgta cggtgaagcc aatcttgctg ccttcaacat tgttccatcc
      301 ccacccagag aaaagaacat gtccaaatct tgataaggct cgctaagtag atgtattttt
      361 tttgattcat ttatctgaaa gatgatattt tcaggtagat agacctctat atttttctca
      421 tttaaggtgc cgattatctt ttttgtaact ctcagatttt tatctttttc ttcgtttacg
      481 attattcctg tttttctaat cttcatctat ttccccttat attattttaa aacaaaaaca
      541 tacattccta acaaggaaat caacattcct aacaaaaaga aaataatatt ttcgactaat
      601 ttgaaatgaa agtagggaag ctgaaatctt atttcggaaa attttttggt atccatatcg
      661 aatagcataa tcccatacgg ctgataggct aagaaatatt ctaataattg tcttctagtc
      721 tctcttttag ttattttatt tttagaacca ctcttcacct caaccacata agtacggcca
      781 ttttttttta ccaataaatc gattcttatc agatagcgat gaagaactgc actctctttt
      841 atgatgagtg gtttacttat ttgcctgtca ataatctgaa aaccgttttt tttaagcaat
      901 tcttcggcct ttttttcggc acgtttactg attactgccc ttctggttgc ctttttgctg
      961 ctgagactat tttttatttt gagatataga acgagggaaa gaatacctcc taacaataac
     1021 ataactataa acagatacaa ttctttattt ttaatcataa aaaacaatga tactactata
     1081 cctgtaaagg agtaaatttc ataaaagaaa tgctaaacat gagtttcctt ggcaaaaaaa
     1141 tagtgatggg cttcatctac aatctttttg agcaaattaa tatttgcatc agataaatta
     1201 tctttcccta tttttttttc taaataaagg aaatattcta tgttgccagg agaatttttt
     1261 ataggagaaa aggtcatatt tagtaaatgg aattgatttt ttattttttc taaaagctta
     1321 ctgattacct ccttatgaac ctctgggtca tccacgattc cgcccttttt taccaatact
     1381 ctacccgctt caaattgtgg ttttactaaa gcaataacct ctccattgtc ttttaataat
     1441 tcagctaata caggaagaac cttttctaag gagataaagg agacatcgac agtggctaaa
     1501 tcaaacagaa aattaaatct attcttttgt agatatctaa tattggtctt ttccaaattt
     1561 gttaccctat catcctgacg taaagcccaa tcaagttgac catagcccac atccacacaa
     1621 tatacctctt cagcaccata tttaagcagg caatcggtaa acccaccggt ggaagctcct
     1681 acatctataa ctatctttcc attgacatcc acttcaaatt cagttaaggc ttttttaagc
     1741 ttaaatccac ctctgctaac aaaagggtaa ttatcttttt ctatatttat tttactttct
     1801 atattacatc tggtaccagc tttgtttatt attattccat tcacttttac cagattagcc
     1861 ataatatatc ttttagcttt ctctcgagaa tcagttaatc ccatatcaac taataattta
     1921 tccaaccgtt ctttttttat catcatttaa atatcataat aaaactaata attatttttt
     1981 aagccagctt tcatctcgct ccagaggaat gctggtatca acgtggccta ccaaagtctc
     2041 tccgctattc ccatcaacaa gtatttgtac tttgttaatt tcagggaaag aagttaaagt
     2101 cattactatg gaataaactg tcaataattc ccctgtactt ccacctggat ggttctttat
     2161 tacttctgag gaaagatcga tataggcaat accatcacca atatacaaac cattaactct
     2221 ggtagtagag ggtatagtgg ggtaaagctc actgcttgac ggacccttaa taagctctat
     2281 tattgcctgt ttggctattc cctcaactat ctttatcttt ctatattcgg gaaccaggta
     2341 ttgcgcattt ccatcggcaa aatataacac aatctgttct tccccaacta catcagatgg
     2401 gggtgtcaac tcaatttctt cctttgtttc ttctatttta ggcacccttt tttccaggat
     2461 taaagggata ataaattttt gcattagaaa gatgataata accaataaaa taaccaatac
     2521 tgcaaagtca attattgatt taaaagaatt ttttttcttt cttcttctcg ccatttcaag
     2581 cctctgttta aatattatca ggaatatttt ttacataatc ctttattgac cgggtaagag
     2641 cctgggctac tttttctctg aattcaggcg tttttaacag cttctcctca ttgggattgg
     2701 agatgaaacc aacctcaatc agcacagcag ccatattcac cccttttaaa acaataaaag
     2761 gagcttgttt aacgcctcta ttagtcaaac cagtatttcg tactatgttt ccctgaacta
     2821 ttttggcaaa ctccgtacta aatcttatat ttacactttc ccccaaatca taaaggattt
     2881 tatttaatag atccgtttct tgatgataga attgacttgc tctattttct cggtcagcaa
     2941 catctttagc tgatgcatca gaagcatatt tcgaatttaa aaaataagtc tcaattcctt
     3001 tagcagtagg ggaacctttt tgtatagctg aattggtatg aatactaata aacacagttg
     3061 cctctttctc catagcaatt aatggtctct tttccaatgg aacatcaaca tctttttccc
     3121 tagtaagata aactttgaaa ccattttctt gtaataattt cttcgccctt aaggcaatat
     3181 caagaactat atctttttcc ttcagtcctc ctggcccgat tgctccaggc tgattacctc
     3241 catgtcctgg atctaataca ataacataat cggtattctt ttgaacacct tctgttaacg
     3301 tttctgattg aatttcagtt tgagaaacaa tgtccatctg actcagggga gcaaatatat
     3361 ctactactat acgttctggt gaagataaat tgaatacttc aaattcatat ttccctttta
     3421 aatctgcaac aatacgtact ttttcgggag taaactgacc acttcttact cttttcacca
     3481 ataaatcatc aacatttaat tcttcttctt ttttattcaa ttgcttcaga gtggcatcaa
     3541 tatcaactac taatctttca ggttctttta gggttagaac atgataggaa ctcttttctg
     3601 atatatcaat aactatcctg gttttatccg gatgagaaaa actacgaata gaagttacct
     3661 gaggtttatg actggtaata attatttctt ttgaagc
//
LOCUS       DAOQWT010000097         3646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_26397_length_3646_cov_3.243386, whole genome shotgun sequence.
ACCESSION   DAOQWT010000097 DAOQWT010000000
VERSION     DAOQWT010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26397_length_3646_cov_3.243386"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..2480
                     /gene="pheT"
                     /locus_tag="PKJ95_02770"
     CDS             42..2480
                     /gene="pheT"
                     /locus_tag="PKJ95_02770"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNR65200.1"
                     /translation="MQLSYNLLKEYVDIALTPEELAERLSMNGIVAERIKPVFEEVKG
                     VVVGEITDIQAVGKSGNLSLCRVDIKSKKLEVICGAKNIRVGNFVPVVIEGGNLPGLG
                     KIEGKEIQGVYSQGMICSAWELGLEKSKSPGVLILENNFPLGENIKNIKDIADDVIFD
                     FEIFSNRPDLMSIIGIAREIAAFSGKPLHIPEQELIEIEEFITDYISVEVEDESLCPR
                     YAGRIIKDLQVEESPFWLRWKLYLLGVRPINNIVDVTNYVMMETGQPLHAFDLSLING
                     KKIIIRRANPGEKFRTLDGVERVLTVDNLVIADTKRAIALAGVMGGENSEIKANTKDV
                     FLESAYFDPINNRKTSSYLGLRTDASNRFEKGIDPYGQVFALNRSAYHIMRLTEGKVI
                     SGVIDQYSKELSKGKAIKLNLCKIDQVLGMKVGKTNKERKERVIDILSKLKFQINSLE
                     EHLLEVTPPSFRSDIKIDVDIIEEIARIFGYENIPSTLFKATLVQEGKSNKQKAVDKI
                     RNTLISCGMHQIISYSMINPKYFEWLKLPSSHHLCKAIELANPLIQEQTIMRTTLIPG
                     LLKAMQWNVNHSIEQIKLFEIGRTYFPRNGAQGGPLPVEKLMITGGIAKIGRGDIWQK
                     SESWDIFYLKGILESLFDSLGIQDVEYLPGDLPFFHPLHNGIITSKGKRIAVFGRVGD
                     DIISYLDIPGNILLFELDYEELYPFIKREIIFQPLPKYPHIQRDMAIIVAQEISFARI
                     KKEVLKISPGIKKRIELFDEFRGKQIKEGFKSIAFSIFFQSDERTLTDTEVDKIMENV
                     TISLKKLFQADLRQ"
     gene            2536..3057
                     /locus_tag="PKJ95_02775"
     CDS             2536..3057
                     /locus_tag="PKJ95_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNR65201.1"
                     /translation="MLIIILFNVFLGFKRGIIAELLTLIGLVTAIFVSIFWYHNLSVF
                     LIRQFKWNEALSNIISFIIIFLLVIFCFRILEDLLHRIVDLLFLSWINNLGGALFGFI
                     RGAIIVSLLLSLVNFIPLPLELRIQLSDSVLAKYLLDSLIIIYSSLREWLPEHFQFEI
                     EFLRENLYKNIGV"
     gene            3108..>3646
                     /locus_tag="PKJ95_02780"
     CDS             3108..>3646
                     /locus_tag="PKJ95_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65202.1"
                     /translation="MMDKHLLEILDYFKIKKLLERELFTFSGKELLQELRPSTDISIV
                     EQWQKKTTEMKNVLERTGRLPLTPLEKDISSDIKRAQIQDSVLNAKDLAYIAIVLEYS
                     HHIKEFLEKLPETEFPLLKENSSAIKYFRVVERTIKDCINEEAFIVDNASPVLKKIRQ
                     KIGSIEKKIRDRLEGIIKDP"
BASE COUNT         1267 a          526 c          753 g         1100 t
ORIGIN      
        1 aattcaaata gaatggtcat tgtgtaggag gaattaattt tatgcagctt tcatacaatt
       61 tattaaaaga atatgtagat attgctttga cacctgagga attagccgaa cggctttcta
      121 tgaatggcat tgtagccgag cgtataaaac cagtatttga agaagtgaaa ggggttgtag
      181 taggagagat aaccgacatt caagctgttg gtaaaagtgg caatctgtcc ctttgcaggg
      241 tagatataaa gagtaaaaaa ttagaagtaa tttgtggtgc caaaaatata agggtaggta
      301 attttgttcc ggtagtaatc gaaggtggca atttacccgg cctgggaaaa attgaaggga
      361 aagaaattca gggagtttat tctcagggta tgatctgctc ggcctgggaa ttgggattgg
      421 agaagagtaa atccccgggt gttttaattt tagaaaacaa tttcccttta ggagaaaata
      481 ttaaaaatat aaaagatatc gctgacgatg taatctttga ttttgagata ttctccaatc
      541 gccctgatct aatgagtata attgggatag ccagggaaat tgcagccttc tctggcaaac
      601 ctctccatat tcctgagcaa gaactaatag agatagaaga gtttataaca gattatatat
      661 cagtggaagt agaggatgaa tccctttgtc cccgctatgc gggaagaatt attaaagatt
      721 tgcaggttga ggaatcgcca ttctggctaa gatggaagct ctatttactg ggtgtcagac
      781 caattaacaa tatagtagat gttactaatt atgtgatgat ggaaaccggt caaccccttc
      841 atgcctttga tttgagtctt attaatggta aaaaaataat aattcgtaga gcaaatcccg
      901 gagaaaaatt caggacattg gatggagtgg aaagggtact aactgttgat aatcttgtta
      961 ttgcagatac caaacgggct atagccttag ccggggtgat gggtggagaa aattctgaaa
     1021 ttaaagccaa tactaaagat gtttttcttg agtcagcata ttttgatcct attaacaaca
     1081 gaaaaaccag cagctattta ggcttgagaa ccgatgcctc taatcgtttt gaaaaaggaa
     1141 tagaccccta tggtcaggtt ttcgctttaa ataggtctgc ctaccatatt atgagactta
     1201 ctgaaggtaa agttatctcc ggagttattg accaatacag taaagaattg agcaagggga
     1261 aggcaataaa attaaattta tgcaaaattg atcaggtatt aggaatgaaa gtaggtaaaa
     1321 ccaataaaga gaggaaagaa agagtaatag atattttgtc taaattgaaa ttccagatta
     1381 attctttaga agaacatctc ttagaggtta ctcctcccag ttttaggagt gatataaaaa
     1441 tagatgtaga tattatcgag gagattgcca gaatatttgg ctatgaaaat attccttcta
     1501 cactatttaa agcaacttta gttcaggaag gtaaaagcaa taaacaaaaa gcagtagata
     1561 aaatcagaaa cactctgatt agttgtggaa tgcatcagat aattagctac agtatgatta
     1621 atccgaaata ttttgaatgg ttgaaattgc catcttccca ccatctctgt aaggcaatag
     1681 agttggccaa ccctttgatt caggaacaga ccattatgag gactacttta atacccggtt
     1741 tacttaaggc aatgcagtgg aatgtcaatc atagtattga acaaataaaa ctatttgaaa
     1801 taggaaggac ttatttccct cggaacggag ctcagggggg acctcttcct gttgaaaaat
     1861 tgatgataac cggcggaatt gctaaaattg gcaggggaga tatctggcaa aaatcggaaa
     1921 gctgggatat tttttattta aaaggaattc tggaatcact ctttgattcc ctgggaattc
     1981 aggatgtaga atacttacct ggagatttgc ccttttttca tcctctacat aatggtatta
     2041 ttacaagtaa agggaaaaga attgcagttt ttggtagagt aggggatgat attataagtt
     2101 atttggatat accggggaat atattactgt ttgaattgga ttatgaagaa ctatatccat
     2161 ttataaaaag agagattatc ttccagccat tacctaaata cccacatatt caaagggata
     2221 tggctataat agttgcccag gaaatcagtt tcgcccggat taaaaaagaa gttctaaaga
     2281 ttagtcccgg tattaagaag agaatagaac tattcgatga attcaggggg aagcagatta
     2341 aagaaggttt taaaagcata gctttctcca tatttttcca atcagatgag agaaccttga
     2401 ccgatactga agtcgacaaa ataatggaaa atgttacaat aagtttgaag aaactttttc
     2461 aggcagattt gcggcaataa ttttcttaaa aagagggggc ctcaggcaat cgttataaac
     2521 tggattgact tagtaattct tataattatc ttatttaatg tgtttctggg ctttaaaaga
     2581 ggtattattg ctgaattatt aacactgata ggtttagtaa ctgctatttt cgtctctata
     2641 ttctggtatc ataacttgag tgtttttctt atcaggcaat tcaaatggaa tgaagcttta
     2701 tcaaacataa tcagctttat tattattttt cttctggtta tattctgttt tagaatactg
     2761 gaggatttat tacatcgaat tgttgatcta ctatttttaa gctggattaa taacttagga
     2821 ggtgccttat tcggatttat tagaggtgct attattgtaa gcctgctctt atccctggtc
     2881 aattttatac ccctaccttt ggaattgaga attcagttat cagattcagt tctggcaaaa
     2941 tatctattag atagtctgat aatcatttat agttcattac gagaatggct gcccgaacat
     3001 tttcaatttg agatagaatt tttaagagaa aatttatata agaatatagg tgtttaaaaa
     3061 attacaagaa ggtcttgaag gaatttactt cctgatggta tatgacaatg atggataaac
     3121 atcttttgga aatattggat tattttaaga taaaaaaatt attggaaagg gaacttttta
     3181 ccttttccgg gaaagaactg ttacaggaac tccgaccatc cacagatatt agtattgtgg
     3241 aacaatggca aaaaaagacc acagagatga aaaatgtact ggaaagaaca ggacgtttac
     3301 cccttacacc actggaaaaa gatattagca gtgatataaa acgagctcag atacaggata
     3361 gcgtactgaa tgccaaagac ttagcatata tagccatagt cctggaatac tcccatcaca
     3421 taaaagaatt tttggaaaaa ctaccagaga cagaattccc tttacttaaa gagaatagct
     3481 ctgccataaa atattttaga gtagtggaga gaacaatcaa agattgcata aacgaagagg
     3541 cctttattgt ggataacgcc agtcctgtgc tgaaaaagat tagacaaaaa ataggaagta
     3601 ttgaaaaaaa aattagagat aggctggaag gcataataaa ggaccc
//
LOCUS       DAOQWT010000098         3573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_27166_length_3573_cov_2.572200, whole genome shotgun sequence.
ACCESSION   DAOQWT010000098 DAOQWT010000000
VERSION     DAOQWT010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27166_length_3573_cov_2.572200"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /gene="selD"
                     /locus_tag="PKJ95_02785"
     CDS             <1..549
                     /gene="selD"
                     /locus_tag="PKJ95_02785"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNR65203.1"
                     /translation="QEGDSLILTKPLGTGVISTALKAAEITISEAQEAIRWMIKLNNI
                     SDHILNLPINSMTDITGFGFLGHLNEMLKDSELGADIEISEVPLLEKALYLSEKNIVP
                     GGSLLNQKFFSCLVEKKINLDFAKEILFYDAQTSGGLLISIRERDCEYLLELLKREGF
                     SESRVVGNIRKNGANDKKIRLV"
     gene            632..1696
                     /locus_tag="PKJ95_02790"
     CDS             632..1696
                     /locus_tag="PKJ95_02790"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNR65204.1"
                     /translation="MPKKRFIIIVFTILSIFLVVGFLVNKMSNRYSGPPILAVSEEIG
                     DLGNINPDKQQSHIFTLKNEGGELLIIERIQAPCSCTATVLEDEKLLPGKTTQLEVTF
                     NPRGYEGEITQSVYIYSNDPENSRKRIAIKANIEHSPSPKIVLSANRWNLGLLSNGDN
                     SAFQVTLSNNGDLVLEIENIVLPNEVHYSQETPEFPIQLAPDEARELSFIYDSSNQEI
                     GVVTEYIRLVTNDPNKKNITLRIEGYIKEKEETISILPLDKFILTGELGKQTYQAKLL
                     IKNNSDKKWKQISLMPSQDYITLNYEEINLSPREEKEIVVTIKQQDFADIDIKEEVQE
                     YIYIKIPIPVNIVIDNPEQF"
     gene            1735..2532
                     /locus_tag="PKJ95_02795"
     CDS             1735..2532
                     /locus_tag="PKJ95_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNR65205.1"
                     /translation="MLNPLLLWRKILMQWIFPPGIIIIAILLLYFLLILRKKKAATIF
                     LIFIVSLLFIFGSWFGEYLFLRPLEEKFILDSDLSGENISLKQPIIVVLSGDSSSGSL
                     LTDECVSEVGEITLARLVGAYLLYKKIECPILVSGGIIPGIGEVTPAASIMGEVLMEL
                     GVTAENILKESKSSTTLENAEFTLNILTRLPFQEVILVTSAVHMPRAMLAFQNDVVTI
                     LPAPVNFLYEDKSPGVLDIFPNRSSWEHNLRAIHEWIGLIYYKIIKR"
     gene            3029..3217
                     /locus_tag="PKJ95_02800"
     CDS             3029..3217
                     /locus_tag="PKJ95_02800"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HNR65206.1"
                     /translation="MPILQIEILKGRTVEQKREMARRVTEAVSETLNCPKEAVSIIIR
                     EMETENFAKAGKLRVDVQ"
     gene            3334..>3573
                     /locus_tag="PKJ95_02805"
     CDS             3334..>3573
                     /locus_tag="PKJ95_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNR65207.1"
                     /translation="MIIIAFFTWIANQSIKLILFYITEKKWDSSRFFGAGGMPSTHSA
                     LSMSVAVSIGYREGWDSALFALASVFTIVVMADAAG"
BASE COUNT         1166 a          547 c          725 g         1135 t
ORIGIN      
        1 caggaagggg atagtttaat cctgacaaaa cccctgggca ctggagtaat ttctactgcc
       61 ctgaaggctg ctgagattac catatcagaa gcacaggaag caatcagatg gatgattaag
      121 ctcaataata tttctgacca tattttaaat ttgcccatta atagtatgac tgatattacc
      181 ggattcggtt ttctggggca tctcaatgaa atgttaaagg atagtgagct tggtgctgat
      241 attgagataa gtgaagttcc gctcttagag aaggcgttat atctttctga gaaaaatatc
      301 gttcccggag gcagtctgtt aaatcaaaaa ttcttttctt gtttagtgga aaaaaagata
      361 aaccttgatt ttgcaaaaga aatattattc tatgatgctc agacttccgg tggacttctc
      421 atctcaatca gggagagaga ttgcgaatat ttgttagaat tattgaaaag agaaggattt
      481 agtgagagca gagtagtggg taatatcaga aagaatgggg caaatgataa aaaaataaga
      541 ctggtctaaa atttattagg cagtccccct aaaatatgat ataaaattgt tctttatatt
      601 aaatctgtta aagaaagagg ttgaagtaat aatgcctaag aagaggttta taataatagt
      661 attcaccatt ctatctatct ttttggtagt cggtttcctg gtcaacaaga tgagtaaccg
      721 atattctggt ccgccaatat tagccgtttc agaggagatt ggagatttag gaaatataaa
      781 tcctgacaaa caacagagcc atatatttac cttaaagaat gaggggggag aactccttat
      841 tattgagagg atacaggctc cctgtagctg tactgcaact gtgctggaag atgaaaagct
      901 tttacctgga aaaacaactc agttagaggt tacctttaac cccagggggt atgaaggcga
      961 aataacccag tcggtatata tttatagtaa tgacccggaa aattcccgca agagaattgc
     1021 tatcaaagcc aatattgagc attccccttc cccaaaaatt gtcctatctg ccaatagatg
     1081 gaatctgggg ctgctttcca acggcgataa ttctgcattt caggtaacct tatccaataa
     1141 tggcgattta gttttagaaa ttgaaaatat tgttcttccc aatgaagtac actatagcca
     1201 ggaaacgcct gaatttccaa ttcagttagc tcccgatgaa gcaagagaac ttagttttat
     1261 ttatgattcc agtaatcagg aaataggcgt ggtaacagaa tacattcgtt tggtaacaaa
     1321 tgaccctaat aagaaaaata ttactttacg tattgagggt tatataaaag aaaaagaaga
     1381 gaccatctct attttaccct tagacaaatt catcttaact ggagaattgg gaaaacagac
     1441 atatcaagcc aaattattga taaagaataa tagtgataag aaatggaagc aaatttctct
     1501 gatgccttcc caggattata ttaccctgaa ttatgaagaa atcaatctat ctccccggga
     1561 ggaaaaagag atagttgtca ctataaaaca gcaagatttt gctgatattg atattaagga
     1621 agaagttcag gaatatatat atattaagat accaataccg gtgaatattg ttattgacaa
     1681 ccctgaacag ttttaacaga gcaaagaatc aaaaaagagt tcaaagagag caagatgtta
     1741 aatccattac tcttatggcg caagattctt atgcaatgga tatttccacc tggaattata
     1801 attattgcca ttttgcttct ctactttctg ttaatccttc gaaagaagaa ggcagccacc
     1861 attttcttga tatttatagt ctccctttta tttatatttg gttcctggtt tggggaatac
     1921 ctctttttaa gaccattgga agagaaattt atccttgatt cagatttatc cggagaaaat
     1981 ataagcctta aacaaccgat aattgtagtt ttatctggag attcatcctc tggaagtctt
     2041 ttaactgatg aatgtgtatc tgaggtagga gagattaccc tggcacggtt ggtgggagca
     2101 taccttcttt ataagaaaat agaatgtcct attctggtta gtggagggat tattcctggt
     2161 attggtgagg taacaccggc agccagtatc atgggagagg tattaatgga attgggagta
     2221 accgctgaga atattctcaa ggaaagcaaa tccagcacta ccttggaaaa tgctgaattt
     2281 actttaaata tacttaccag gttacctttt caggaagtta ttctggtaac cagtgctgta
     2341 catatgccca gggcaatgct ggcttttcaa aatgatgttg tgacaatttt acctgctccg
     2401 gtaaattttt tgtatgaaga taaatcgcct ggtgttttag atatttttcc taaccggagt
     2461 tcctgggaac acaacttaag agcgatacat gaatggatag gattaattta ctataaaata
     2521 atcaagagat agcatatcag aatcagtcgt tggtatttag tatttagttt taaaatgtaa
     2581 agaaaaagag tgtgggcgat agagcatgtg attgccgcgt cgctttgctc ctcccaatga
     2641 cagagtggag gagctcctca tgatgacaaa tttgtcgtac tttttgtgtc gtgcatttat
     2701 atcagtgatt gtcgttggtt ttaataagtt tttggattat agtttttagt cagagaaaag
     2761 agagtttctt ttttatctat tttttgttaa tcttcactga ttactgatta ttaatcattc
     2821 attacaattt tatgtgggtt gcagatgatg agattttcac atcgcttcgc ttcttattgc
     2881 ccttgctaat ttctgaataa aatatatcac aggctgaatt tttttgacca aaactggtat
     2941 ttttgctata aaatagtaac ataatctgaa ttaatattat tatttaaggg cgacattttg
     3001 taaattataa aaaaggaggt tttttattat gcccatttta cagatagaga tattgaaagg
     3061 caggacagtg gaacaaaaaa gagagatggc aagacgagtt acagaagctg tatctgaaac
     3121 tcttaattgt ccgaaagagg cagtaagcat tattatcaga gagatggaaa cagaaaattt
     3181 tgccaaagct ggcaaattga gagttgatgt ccaataataa caatttttca actccacctg
     3241 ttcagggagg gtaatatatc tacttataat aattaaagga gatataaaaa tatcaggttt
     3301 attaatgagt taattgctat tttcaataat aaaataataa taattgcctt ttttacctgg
     3361 attgctaatc agtctatcaa attaatcttg ttttatatta cagaaaaaaa atgggattca
     3421 agcaggtttt ttggagcagg aggaatgccc agcactcatt ctgccctttc aatgagtgtg
     3481 gcggtttcta ttggttatag agagggctgg gattctgccc tgtttgcttt agcgagtgta
     3541 tttactattg tagtgatggc tgatgcggct ggg
//
LOCUS       DAOQWT010000099         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_27261_length_3565_cov_2.764103, whole genome shotgun sequence.
ACCESSION   DAOQWT010000099 DAOQWT010000000
VERSION     DAOQWT010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27261_length_3565_cov_2.764103"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKJ95_02810"
     CDS             <1..208
                     /locus_tag="PKJ95_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65208.1"
                     /translation="NVMGSMEEISKKILTEKSNRLEISFNDAQDTSQLIDSIQRLEGV
                     LKVEKRAEDISLERIYTKYFQEGS"
     gene            211..1152
                     /locus_tag="PKJ95_02815"
     CDS             211..1152
                     /locus_tag="PKJ95_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR65209.1"
                     /translation="MSGLSVIYWKELADYLGSRKFIILFLIVTLTAGTIIYVIHQSVS
                     SDVNQFTGEYLFLKLFTFSAGTLPSFIFFVSFFGPLIGIIFGFDAVNSERSGGTMSMV
                     LAQPIYRDALINGKFLAGITTIAIMLISIIVIISGLEMTVFGIIPTLQELFRIILFSL
                     LSIVYIGFWMGLGILFSILFRQTATSALVSIMVWIFFSFFILMVANVIADQMAPLGNN
                     ASLELIAKNEGIRNMILRVSPLILFQECTTAILNPSVRFFGVTTALKNLEVMPSPLSI
                     GQSVMVVWPQFIILIALILVCFAVSYVVFMRQEIRST"
     gene            complement(1264..1923)
                     /locus_tag="PKJ95_02820"
     CDS             complement(1264..1923)
                     /locus_tag="PKJ95_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008425487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNR65210.1"
                     /translation="MIVEMNKNTLPTASYQKWLDEKINEIKNKQHTPELLLHSCCAPC
                     SSYVIEYLSPFFSITVFFYNPNIHPEEEYKKRLTEQISFIGKLPIKNRVKFKEGPYEP
                     ELFFQKVKGLEQEKEGGRRCLKCFELRMEKTAQVASQLGFTYFTTTLTVSPHKESSSI
                     NQMGGTIAPAYGIEYLFSDFKKRAGFQRSIELSHQYQLYRQDYCGCIFSRQQEKIMDR
                     K"
     gene            complement(2074..2811)
                     /locus_tag="PKJ95_02825"
     CDS             complement(2074..2811)
                     /locus_tag="PKJ95_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65211.1"
                     /translation="MRKLIAFFLSISLFLLLIMGISLASDYLAQADAVYQKDNLESVI
                     ESLPLYRKAVEENPESYEANWKMARALREYADLSMQAEVSDWKDICKVYAKEGLKFAA
                     IAKELEPNKVEGHLYYGLSAGSYSDGVSILKAIKEGLKNKTEKAFEKAYEIDKMYDDA
                     APILALARMWHQLPWPYQKEKTSEKYFEEYYQYFPNNPQGLVYYAELLKDRGKKEQAI
                     VLLKQAAQSDHAYFSKRAKELLDKWSK"
     gene            3171..>3565
                     /locus_tag="PKJ95_02830"
     CDS             3171..>3565
                     /locus_tag="PKJ95_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNR65212.1"
                     /translation="MLKTIPVNTSNRTEMLDITAEVERVVQESNLKEGLCIIFVPHTT
                     AAVTINENADPDVLVDILQELEKVIPFNDDYRHLEGNSAAHIKSSLIGSSISVIIENG
                     HLKLGTWQGIIFCEFDGPRTRDVWIKIIS"
BASE COUNT         1059 a          634 c          663 g         1209 t
ORIGIN      
        1 gaacgttatg ggttcaatgg aggagataag caaaaagata ttaaccgaaa aaagtaaccg
       61 attagaaata agtttcaatg acgcccagga tacctcacaa ctcattgaca gtattcaaag
      121 actggagggt gtcctgaaag tagaaaaaag agctgaggat atctccctgg aaagaatata
      181 tactaaatat tttcaggagg gtagttaatt atgtctggct taagtgtaat ttactggaag
      241 gaattggcag attatttggg cagcaggaaa ttcataattc tctttttaat tgtgactcta
      301 actgccggta ctatcattta tgtgatacac caatcggtaa gcagtgatgt aaaccagttt
      361 accggagaat atctctttct gaaattattt actttttctg ctgggacact tccttctttt
      421 atcttttttg ttagtttttt tggtcctttg ataggaataa tctttgggtt tgatgcagta
      481 aacagtgagc gttccggtgg aactatgagt atggtgttag cacagcccat ctaccgggat
      541 gctctgatta acggtaagtt tttagcaggg ataactacta ttgccataat gctgatcagt
      601 attattgtga ttataagcgg tttggaaatg acggtatttg gaattattcc tactttacag
      661 gaactatttc gtataatact attctccctg ttaagcatcg tttacatcgg attctggatg
      721 ggtttaggaa ttcttttttc catattgttt agacaaacag ccacttctgc tttagtatca
      781 ataatggtgt ggatattttt ctctttcttt atacttatgg ttgccaatgt tattgccgat
      841 cagatggctc ccctgggaaa caatgcctct ttagagctca ttgccaaaaa cgagggtatt
      901 agaaatatga tcttaagggt atcgcccctt atacttttcc aggaatgtac cacagccatt
      961 ttaaatccct ctgtcaggtt ttttggtgta accactgcct taaagaatct ggaggtgatg
     1021 cccagtcctc tcagtatcgg gcagagtgtt atggtggttt ggccacaatt tattattctg
     1081 attgccttaa tcctggtatg ttttgcagtc tcttatgtag tatttatgcg tcaggagatt
     1141 cgctccacct agttatcatg atataagata gtaatgatat atatagtttg aaacggttgt
     1201 tttaagctct tgaccttaaa caaaataaga aaatgaggag gtgcagtttt gaaatgtacc
     1261 tcctcatttt ctatccataa tcttttcctg ctgcctggaa aagatacatc cgcaatagtc
     1321 ttgacgatag agctgatatt ggtgagaaag ctcgatagag cgttgaaaac cagccctctt
     1381 tttaaaatca ctgaataggt attcaatacc ataagcggga gcaatagtgc cgcccatctg
     1441 attaatcgaa ctgctctctt tatgcggact gacagttaga gtagttgtga aataggtgaa
     1501 acccaattga gaagctactt gtgcagtctt ttccatccgc agctcaaaac actttaaaca
     1561 acgtctccca ccttcttttt cctgttccag tcctttcact ttctggaaaa aaagttccgg
     1621 ctcatagggg ccttctttaa attttaccct attttttatg gggagtttcc caataaacga
     1681 tatttgctct gttaatctct ttttatattc ttcttcaggg tgaatattgg gattatagaa
     1741 aaatacggtt attgaaaaaa aaggagaaag atattctatt acataactgc tgcagggggc
     1801 gcagcagctg tgcagtaaaa gctcaggcgt atgctgttta ttctttatct cattgatttt
     1861 ctcatcaagc catttttgat atgatgctgt tggtaaagta tttttattca tctctactat
     1921 cataacatag gaaaagcatt aattgtattg tatagagttt gttttagaat ttaaaaattt
     1981 ttgataatat agcaatagct tcgtaaatag agttgatacc atgcataata atatccctta
     2041 cgaagctatc ccatactatt ttttaaaaag cgattacttg gaccatttat ctaacaattc
     2101 ttttgccctc ttgctgaaat aagcatgatc gctctgggca gcctgtttta agagaacaat
     2161 tgcctgttct tttttaccac ggtcttttaa tagctcggca taatagacca gaccctgagg
     2221 attatttgga aaatattggt aatattcttc gaaatacttt tcagaggttt tttctttctg
     2281 gtaaggccag ggcagttgat gccacattct tgccagagcc agtatgggag ccgcatcatc
     2341 atacatctta tcaatctcat aggctttttc aaaagctttt tcagtcttat tttttaatcc
     2401 ctccttgatt gcttttaaga tgctgacacc atcagaataa gaaccggcac tcaatccata
     2461 atagagatgt ccttccactt tattaggctc taattccttg gcaatagcag caaacttcag
     2521 gccctctttg gcatatacct tgcagatatc tttccagtct gatacctcag cttgcattga
     2581 cagatcggca tattctctta aagcacgtgc catcttccaa ttagcttcat agctctccgg
     2641 gttttcttct acagcttttc gataaagagg aagagattct attacacttt ccaggttgtc
     2701 tttctggtaa actgcatcag cctgagcaag atagtctgat gccaggctga tacccattat
     2761 taatagtaaa aataatgaga tgctcagaaa aaaagctatt aattttctca taaaacctcc
     2821 ttgataagga aatattttaa tattagtagt agctcttaat acatagtatt ataacaaaaa
     2881 tattaggaca gtcaactcta tttttaaacc aaaaaactgt caatttttcc tgattttgct
     2941 caagttaagt ggtataattt ttaaatacga caatatcatt gcttcaaatc attaaatagg
     3001 gtaagggtat ttataaaata tcttaatcta tgattagggt aaaaaatgtg atagactgga
     3061 atatatttat ttcatattaa taaagtaatt ctttaatttt tcattggtca aattttaagg
     3121 atttaggaac agtttactaa ttatgctaaa aaagagtgaa ggagttagct atgctaaaaa
     3181 caatcccggt taataccagt aatcgtacag aaatgcttga tatcactgct gaagtagagc
     3241 gggtagttca agaaagcaat ctaaaagaag gactctgtat tatttttgtc ccacatacca
     3301 ctgccgctgt cactattaat gaaaatgccg accctgatgt gttggtagat atattacagg
     3361 aattagaaaa agtgattccc tttaatgatg attatcgtca tctggaaggt aattctgcag
     3421 cccatatcaa atcgagtctg ataggctcct caatctcagt tatcatagaa aacggccatt
     3481 tgaaattggg gacctggcag ggaataattt tttgtgaatt tgacggacct cgtactcgtg
     3541 atgtctggat aaaaatcatt tcctg
//
LOCUS       DAOQWT010000100         3559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_27343_length_3559_cov_3.307363, whole genome shotgun sequence.
ACCESSION   DAOQWT010000100 DAOQWT010000000
VERSION     DAOQWT010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27343_length_3559_cov_3.307363"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKJ95_02835"
     CDS             <1..468
                     /locus_tag="PKJ95_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNR65213.1"
                     /translation="EKFLEKMRSADLIVTTFFHFKELQDFLQDENKRIIAIALDPQME
                     TMVQIAHSTSPDMNIGLVCLTNNFAERVIKSINNVGIKYKKIIFTTTRNSIELKKIIN
                     DSDILIVSPGRKKEIQGLISKNVPLIEFIYVPDKGSIETLRNNIFEIKKKEVT"
     gene            475..747
                     /locus_tag="PKJ95_02840"
     CDS             475..747
                     /locus_tag="PKJ95_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNR65214.1"
                     /translation="MDKKNNLKVYQNEKTVITVNEDWCKSCGICIHFCPKKVLVSNEK
                     GFPEAKNIDDCIHCMLCELRCPDFAINVEKKEKTVEHIVNQKGLED"
     gene            753..1898
                     /locus_tag="PKJ95_02845"
     CDS             753..1898
                     /locus_tag="PKJ95_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNR65215.1"
                     /translation="MDKNRSVRILQGNEACVEGALLAGVRFFAGYPITPASEIAEGMA
                     VRLPELGGVFMQMEDEIASMAAVIGASLAGSKSMTASSGPGICLKQENIGFAAITEVP
                     CVVVNAQRGGPSTGLPTKPSQGDVMQARWGTHGDHPMIALAPSDVNEVLHMTIKAINF
                     SEKYRTPVMLLLDEVISHMREKVNIPTREEIELIDRKKPAVEKDNFLPYKADEDLVPP
                     MANFGSGYRYHVTGLIHNEKGYPTSNNKEIDQLIRRLTNKVELNKKDIIIEEEFLLDD
                     AKVAIIAYGSVARAAQRTIKLAREKGIKTGLLKLITLWPFGDETINRLAGQVDLIIVP
                     EMNLGQMSREVERAVGGKCRVVSYSRVDGELINPLEILTRIMEESKL"
     gene            1895..2722
                     /locus_tag="PKJ95_02850"
     CDS             1895..2722
                     /locus_tag="PKJ95_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNR65216.1"
                     /translation="MNNSITQYFRKEMFPHIWCPGCGIGIILGSMLRAIQSTGWEKDE
                     VVMVSGIGCSSRITGYVDFNTMNTTHGRALPFATGIKMANPALKVITVMGDGDCAAIG
                     GNHFIHSARRNIDITAIIINNMIYGMTGGQYSPLTPTGKLGSTAPYGNIDQSFDIAAL
                     AASAGASYVARGNVFDVNQITHFIKKALLKKGFSVVEIIADCPISFGRINQLRTPVQM
                     LEWIKSITVAKRVWEGLPEEEKAGKIPTGEFLDIDIPEYTEKYQETCNRIQQGRREK"
     gene            2719..3273
                     /locus_tag="PKJ95_02855"
     CDS             2719..3273
                     /locus_tag="PKJ95_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNR65217.1"
                     /translation="MRKEICLSGSGGQGLILAGIILAEAAGIYDGFEIAQTQSYGPEA
                     RGGASRSEVIISNEKIDYPKIGKSDILLALTQKACDKYVNSLKEEGVLLVDSTNVFNI
                     PPFPGKIYQYPITKDAIDVLGNQLVTNIISLGIIVQLTDIVSKDAIKKALQNRIPERI
                     RDINKKALEHGFQIGKNLMNTSRV"
     gene            3300..>3559
                     /locus_tag="PKJ95_02860"
     CDS             3300..>3559
                     /locus_tag="PKJ95_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNR65218.1"
                     /translation="MKILVVNSGSSSIKYRLYDMAEESLLAKGLVERIGIEGSLINHY
                     PSGKDFLKKEIDIPDHRKGIELVIEALLDSRYGVIKSISEISA"
BASE COUNT         1219 a          507 c          786 g         1047 t
ORIGIN      
        1 gaaaaatttt tggaaaaaat gaggtctgct gatcttatag taacaacctt tttccatttt
       61 aaggaactac aagattttct acaggatgaa aataaaagaa ttattgctat agcattagat
      121 cctcaaatgg aaaccatggt tcaaattgcc cacagtacat ctccggatat gaatatcggc
      181 ttagtatgtt taactaataa ttttgctgag agagttataa aatctattaa taatgtcggt
      241 ataaaatata aaaaaataat atttactaca actcgcaata gcattgagct aaaaaaaatt
      301 attaatgata gtgatatatt aatagtctct cccggaagaa agaaagaaat acagggatta
      361 atttctaaaa atgttcctct cattgaattt atttatgttc ctgataaggg ttccattgaa
      421 actttgagga acaatatttt tgaaataaaa aagaaagagg tgacctaata acgaatggat
      481 aaaaaaaata atttgaaggt ttatcaaaac gaaaagaccg taataacagt aaatgaagat
      541 tggtgtaaat catgtgggat ttgtatccat ttttgcccta aaaaagtgct ggtaagtaat
      601 gaaaaaggtt tccctgaggc aaagaatatt gatgattgta tacattgcat gttgtgtgaa
      661 cttagatgcc cggactttgc tatcaatgtt gaaaagaaag agaaaacagt tgagcatatt
      721 gtcaatcaaa aaggtctgga ggattaatca gtatggataa aaacagatcg gtgaggattt
      781 tacagggcaa tgaagcatgt gtagaaggtg cccttttagc cggtgtacgg ttttttgcag
      841 gctatcccat cactcctgct tctgaaatag ctgaaggtat ggctgttcgt cttcctgaac
      901 tggggggagt ttttatgcag atggaggacg agattgccag tatggctgct gtcattggtg
      961 cctctttagc gggctctaag tcgatgaccg ccagttccgg tccggggata tgtctgaaac
     1021 aggaaaacat tggatttgct gcaattactg aggttccttg tgttgttgtc aatgctcagc
     1081 ggggtggtcc gagtaccggg ttacctacta aaccttctca aggggatgta atgcaggctc
     1141 gctggggaac acatggagat catcctatga tagccctggc tccttcagat gtaaatgaag
     1201 ttttacacat gactataaag gctatcaatt ttagtgagaa ataccgtacc ccagtgatgt
     1261 tactgttaga tgaagtaatt tcacatatgc gtgagaaagt taatatacct accagagaag
     1321 aaatagagct cattgacagg aaaaaaccag cagtagaaaa agataatttt ttaccttata
     1381 aagcagatga agatcttgtt ccaccaatgg caaattttgg cagtggatat aggtaccatg
     1441 taacaggttt aattcataat gaaaaaggtt atccaacttc taataataaa gaaatagatc
     1501 agttgatcag aagattaact aacaaagtgg aattgaataa aaaggatatt attatagagg
     1561 aagagttttt attagatgat gctaaagtgg caattattgc ttatggttct gtagcaagag
     1621 ctgcacaaag aaccattaag ctagccagag aaaaaggaat aaagacaggt ttattaaaac
     1681 tgattactct ctggcctttt ggcgatgaaa ctattaatcg attggcaggg caggttgatt
     1741 taattatagt accagagatg aatttgggtc agatgtcgcg tgaagtagaa agggcagttg
     1801 gaggaaaatg tcgtgttgtc tcatatagca gagttgatgg tgagctaatc aatcctttag
     1861 aaattcttac caggattatg gaggaaagca aattatgaat aattctatta ctcagtattt
     1921 tagaaaagaa atgttccctc atatctggtg tccgggttgt ggaatcggta ttattttggg
     1981 ctctatgtta agggcaatcc agagtactgg atgggagaag gatgaagttg tcatggtttc
     2041 aggaataggt tgttccagcc gtattactgg ttatgtagat tttaatacta tgaatactac
     2101 tcatggcagg gctttacctt tcgcaacagg aataaaaatg gccaatcctg ctttaaaagt
     2161 aattactgtg atgggtgatg gagattgtgc tgctatagga gggaaccatt ttatccattc
     2221 tgctcgtcgt aatatagata taactgccat catcattaat aatatgatat atggaatgac
     2281 cggagggcag tactctcctt taaccccaac cggcaagctt ggttcaactg ctccctatgg
     2341 taatattgac cagagttttg atatagctgc attagcggct agtgccggag cttcttatgt
     2401 ggcacgtgga aatgtttttg atgttaatca aattactcat tttattaaaa aggcattact
     2461 taaaaaaggt ttttccgtag tagaaattat tgccgattgt cctatttctt ttggcagaat
     2521 taatcaattg aggactcctg ttcaaatgct tgaatggata aaaagtataa cagtagcaaa
     2581 gagagtttgg gagggattac cagaagaaga aaaagcaggg aaaataccta ccggagagtt
     2641 tttagatatt gatataccag aatataccga aaaataccag gaaacctgta acagaattca
     2701 acagggaagg agagaaaaat gagaaaagag atttgtttga gtggttcagg aggacagggg
     2761 ttgatcctgg ctggaattat tttagctgag gctgctggca tctatgatgg ttttgaaatt
     2821 gctcaaaccc aatcatatgg accggaggct agaggagggg ctagcagatc agaggttatt
     2881 atcagcaatg aaaagataga ttaccctaaa attggaaaat cagatatatt attagcctta
     2941 acacaaaaag cctgtgataa atatgttaat agtttgaagg aagaaggagt tttactggta
     3001 gattcaacta atgtttttaa tattccgcca ttcccgggga aaatttacca atatcccatt
     3061 accaaagatg ctatagacgt tttagggaat cagttggtaa ccaatattat ttctttaggt
     3121 atcattgttc aattaacaga tattgtttct aaagatgcta tcaaaaaagc actacagaac
     3181 agaataccgg aaagaataag agatataaat aaaaaagcat tggagcatgg ttttcaaata
     3241 ggaaaaaatt taatgaatac gagtagggta taatttgtat ttagaaagga aagaatgaaa
     3301 tgaagatttt agtggtgaac tccggtagtt cttcaataaa atataggctt tatgatatgg
     3361 cagaagaatc tctgttagcc aaaggtctgg tggaaagaat tggaatcgag ggttctctga
     3421 taaatcacta tccttcaggg aaggattttt taaaaaaaga gatagacata cctgatcacc
     3481 gtaaaggaat agaactggta atagaggctc tgctggatag tagatatggt gttataaaaa
     3541 gcatttcaga aatatctgc
//
LOCUS       DAOQWT010000101         3547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_27451_length_3547_cov_3.066438, whole genome shotgun sequence.
ACCESSION   DAOQWT010000101 DAOQWT010000000
VERSION     DAOQWT010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27451_length_3547_cov_3.066438"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /gene="deoC"
                     /locus_tag="PKJ95_02865"
     CDS             complement(<1..651)
                     /gene="deoC"
                     /locus_tag="PKJ95_02865"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNR65219.1"
                     /translation="MPEIISLETAKILAHNDIARLIDHTALRPDVTGIEIEALCKEAL
                     QYHFYSVCINPGFIPFARTLLSSSDVKICTVVGFPLGATLSRVKAYEAEESAREGARE
                     IDMVLNISALKDRNYQKVEQEIKMVVKAVSGLICKVILENCLLTDEEKEIGCKISVQA
                     GAHFVKTSTGFEKGGATIDDIKLMRKVVGKHIGVKAAGGIRDYDTALQMVEAGATRI"
     gene            complement(704..1360)
                     /locus_tag="PKJ95_02870"
     CDS             complement(704..1360)
                     /locus_tag="PKJ95_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR65220.1"
                     /translation="MENILETIIDGEKNERQQFSFSTYEAYKKIKKNDPLKMILESID
                     WSFINPLVKDRYYNNKILIYSPVSLFKAQLLIYLGEIDSNRQLAEVLRYNTKYCVLCG
                     FHHFLKTPAHSTFSAFRKRIGSDLFHKIMHRLVAQSVPMISKKLSYISPQTLHLTIYS
                     REGEIISCNCKGKCKMEKIITKNNKEINKKNFVIDEYRVKLYIDRKSYKPFAAELRPK
                     "
     gene            complement(1521..2888)
                     /gene="ftsZ"
                     /locus_tag="PKJ95_02875"
     CDS             complement(1521..2888)
                     /gene="ftsZ"
                     /locus_tag="PKJ95_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNR65221.1"
                     /translation="MKKKKLARGFTKILDYKQELIRDTSQGLEQKPFISDSTDGDGEL
                     VYHEMEKKLPVEESKVESFVNIKVIGVGGSGNNAIEDMANHRINGLALIAINTDLQAL
                     SLSQAGQKVQIGKLTTKGLGAGGDPEVGKKAAEEDRNAIAKVLEDADIVFITAGMGGG
                     TGSGAAPVIAEIAKREGALTIGIVTRPFAFEGKKRMIQAERGIEELQKQVDALITISN
                     DRLLTLIGKDTSVKDAFEITDRVLRQSVQAIADLITTPGLINLDLADVKAVIKDAGFA
                     LVGIGIGRGNNKASTAAEAAISSPLLEVSIKGSNSIIVNVTGGLDMSLYEVNEIVDLV
                     SKAAGRDANIVFGAIISENMQDEVRVTVIATKCEKAMPLPVNQEEVETITIREKVEQE
                     ERKRERISPFFSESLVENPILKDKKFEPDIGKSSAEETVGPAKKYQEKEDLDIPTFLR
                     KKYRR"
     gene            complement(2854..>3547)
                     /locus_tag="PKJ95_02880"
     CDS             complement(2854..>3547)
                     /locus_tag="PKJ95_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNR65222.1"
                     /translation="DPQAHTTIGLGVEPNELEKTMLNVLEPSRSPNKLNLEDVVIQMK
                     KFSNGNLYLAPSNIDFASAEYKLIDQIGREDFLNIGIQNCKIKFDYIIIDCPPSLGIL
                     TINALKASSEVIIPIQPHYFALRGIEEFMETIKIVHQNLHHFLEIHVLITIADTRSNI
                     AKEVVEETRDFFKERVFKTIIRKNISLVESSSQGIPIIYYNPRSHGAEDYSELAQEVI
                     SFEKEKISARLY"
BASE COUNT         1008 a          731 c          609 g         1199 t
ORIGIN      
        1 aatgcgggtg gcacctgcct ctaccatctg caaagcagta tcataatctc tgattccccc
       61 tgctgctttg acacctatgt gctttcccac aacttttctc atcagtttta tatcatcaat
      121 agttgcgcct cctttttcaa aaccggtaga agtcttcaca aaatgggcac cggcttgaac
      181 ggaaatcttg caaccaattt ctttttcctc atcagtcaat aaacagtttt ctaaaataac
      241 tttacatatc aatccggata cagcctttac caccatttta atctcttgtt cgactttttg
      301 gtaatttcta tcctttaagg cagagatatt gagaaccata tcgatttccc tggcaccttc
      361 tctggcagat tcctctgcct cataagcctt aacccgggac agggttgccc ctaaggggaa
      421 tcctaccacg gtacatattt taacatctga ggaacttaac aaagtcctgg caaaaggtat
      481 aaaaccggga ttaatacaga ccgaataaaa atggtactgc agtgcttctt tacagagagc
      541 ctctatctct ataccggtga catccggacg cagagctgtg tggtcaataa gtctggcaat
      601 atcattgtgt gctaatattt ttgcagtctc aagagaaatt atttctggca taatatcttc
      661 tctcctttca ggattataag gggaaaaagt ttttaaaagg taatcatttt ggtctgagct
      721 cagctgcaaa cggtttatat gattttcggt caatgtatag ctttaccctg tactcatcaa
      781 tcacaaaatt ttttttgttt atttctttgt tatttttagt aataatcttt tccattttac
      841 acttgccttt gcaattacaa ctgattattt ctccttccct ggaatatatg gttagatgca
      901 gagtttgggg agaaatataa ctaagttttt tggaaatcat aggaacactc tgggccacca
      961 gacgatgcat tattttatgg aatagatcag aaccaattct ttttctaaaa gcagaaaagg
     1021 tggaatgtgc tggtgttttt aagaagtggt gaaagccgca aagaacacaa tattttgtat
     1081 tataacgcag gacttcggcc aattgccgat tcgaatctat ctctcccaaa taaataagca
     1141 attgggcttt aaaaagagaa actggggaat agataaggat tttgttgtta tagtatcggt
     1201 ctttgactaa tggattgatg aaagaccagt caatcgattc taaaatcatc ttcaaaggat
     1261 catttttctt tatcttttta taagcttcat aggtagaaaa tgaaaattgt tgtctttcgt
     1321 ttttttcccc atcaattatt gtttccaaaa tattttccat ttattaaaaa tattgtaatt
     1381 gtttggtagt aatataagtt tttggtttat ggtttttagt taataacttg tttattatga
     1441 atacgttctt ctaagaagat gagtaatgcc tcaagaaagg aaagaaaata aatataaata
     1501 aaagaaaatt ataaaaaata ctatcttctg tatttttttc ttaaaaaggt aggtatatcc
     1561 aggtcttctt tttcttgata ttttttggca ggacctactg tttcttccgc cgaagattta
     1621 ccaatatcag gttcaaattt tttatctttt aaaatggggt tttcaactaa actttcgctg
     1681 aaaaaaggag aaatcctctc cctctttctt tcttcctgtt ctaccttttc ccggatagta
     1741 atagtttcta cctcttcttg atttacaggt agtggcatag ctttttcaca tttggtagca
     1801 attacggtaa ccctgacttc atcctgcata ttttcagaaa ttattgcccc gaaaacaata
     1861 ttggcatctc tgcctgctgc tttggagacc aggtccacaa tctcgttaac ctcataaaga
     1921 ctcatatcca atccaccggt tacattgact atgatggaat tggaaccttt gatggatact
     1981 tccagtaaag gactggaaat agctgcctca gcagcagtgg aagccttatt attcccgcgt
     2041 cctataccga tacccactaa tgcaaagcct gcatccttga ttactgcctt tacatctgct
     2101 aaatcaagat ttattagccc aggggtggta atcaggtcgg caattgcctg gactgactgc
     2161 ctcaaaaccc gatcagttat ttcaaaggca tctttaactg aagtatcttt gccaatcagg
     2221 gtaagcaaac gatcattgga aatggtgata agggcatcga cctgtttttg aagttcttct
     2281 atcccccgct ctgcctgtat catcctcttt ttcccctcaa aagcaaaggg ccttgtaaca
     2341 attcctatgg taagggctcc ttctcttttg gctatttcgg caataacagg cgccgcgcca
     2401 cttcccgttc ctccacccat gccggcagta ataaagacga tgtcggcatc ttctagtacc
     2461 ttggcaatgg cattcctatc ttcctcagcc gcttttttgc caacttcggg atcaccgcca
     2521 gcgcccaaac ctttagtggt cagtttaccg atttgcactt tttgccctgc ctgggataat
     2581 gagagtgctt gcaaatcagt attaattgca attagcgcca atccattgat gcgatggttt
     2641 gccatatctt caatagcatt gtttccacta cccccaacgc caattacttt gatattaaca
     2701 aaactttcta cttttgattc ttctacaggt agtttctttt ccatttcatg ataaaccaat
     2761 tctccatctc catcagtgga atctgatata aatggtttct gctccaaacc ctgagaagta
     2821 tccctgatta attcctgctt ataatctaaa attttagtaa agccgcgcgc taattttttc
     2881 tttttcaaag gaaatcacct cttgagcaag ttctgaataa tcttctgccc catgagatct
     2941 cggattatag tatataatgg gaattccctg actggaagat tccaccaggg atatattctt
     3001 tctaataata gtcttaaata ctcgttcttt gaaaaaatct cttgtttcct caacaacttc
     3061 tttggcaata ttgcttctgg tatctgcaat agtgatgagg acatgtattt ccaaaaaatg
     3121 atgcagattt tgatgaacaa tttttatagt ttccataaat tcttcaatac cacgcagagc
     3181 aaagtaatgg ggttgaatag gaataattac ctcactggaa gctttcaggg cattgattgt
     3241 taaaattccc agtgaagggg gacaatctat gataatgtaa tcaaatttta ttttacaatt
     3301 ttgtatacct atatttaaaa aatcttcccg gcctatctgg tcaatcagtt tatactccgc
     3361 actagcaaaa tcaatattac tgggagccag atacagattt ccgttagaaa attttttcat
     3421 ttgaatcacg acatcttcca gattgagttt attgggagaa cgacttggtt ctaaaacatt
     3481 gagcattgtt ttttccagtt cattaggctc aactcccaaa ccaatggtgg tatgtgcctg
     3541 gggatcc
//
LOCUS       DAOQWT010000102         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_27738_length_3520_cov_2.797980, whole genome shotgun sequence.
ACCESSION   DAOQWT010000102 DAOQWT010000000
VERSION     DAOQWT010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27738_length_3520_cov_2.797980"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PKJ95_02885"
     CDS             <1..234
                     /locus_tag="PKJ95_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65223.1"
                     /translation="GAYLTTVGEVAGERHSGTATGLALLFVRGAMIVSPPIFGLIADI
                     NGSYRYSWLIYSFVIIGMSFLFLPLKKQPVVLT"
     gene            complement(238..984)
                     /locus_tag="PKJ95_02890"
     CDS             complement(238..984)
                     /locus_tag="PKJ95_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR65224.1"
                     /translation="MKKIKQPFFIVTALLFAFFLISILRFDIIENVLVLLVKPKSFFL
                     ARVSLVEIAKQHIFIVIISSSLSIIIALTIAILTRFPFMSDFEEFLLGLATFGQTIPT
                     VAILALLVPFLGYGFKPALFALFVYGFLPVLRNTIEGFNTIPEEIRESARAMGMNRFQ
                     IITRVELPLALPAILAGIRISLILNISVATIGATVGLSGFGTLIVNGIRGDDTIMILK
                     GAIPVSLLALAVESLFIILEKRFRLSAISE"
     gene            complement(981..1937)
                     /locus_tag="PKJ95_02895"
     CDS             complement(981..1937)
                     /locus_tag="PKJ95_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65225.1"
                     /translation="MIELKNVTKKYNDITALKNFSLQIPEGILQVLLGPSGCGKSTVI
                     KLINHLIEPTSGEIYINNKNINEFNLINLRRGIGYVIQSIGLFPHYNVYENIVIVPRL
                     LKWTKNDIDRRVDYLLDLVGLNRNYKKKYPFELSGGEGQRVGVARALAADPPILLMDE
                     PFGSVDPLNRERLQKEFYRIQRELKKTVLFVTHDVEEAIRLADNIAVMKPGQLASLGS
                     PWDFVTDDKEDYVKEFLGSEYTLKLLTRFMVQDILVQEKIKSKGYIHGFQVKKENTLS
                     SALSLMISKGIDSLEVIDEQEKIVGSITFEDILKNVFRDTRK"
     gene            complement(1934..3100)
                     /locus_tag="PKJ95_02900"
     CDS             complement(1934..3100)
                     /locus_tag="PKJ95_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR65226.1"
                     /translation="MPDSGKKISILGAFILILSFLLLSFAEYRPNRVLSGTNIGAREL
                     FGSGCFLLIFLSILIIFLIITNHKGHKLIITVTSLLIMGLVLYLFGIAIEKETFNIIP
                     NGRISLGIGFWAIFLGMEMVMSGCFKGDKKCILYRRVFYTILFLMVFVFLLSGHLNNL
                     SIMKEFFNRSNTFFRQIRRHFSLSLFSVIAGLIIGIPIGVLIYKERKMKSTILFLINL
                     GQTIPTLSLLGIIMVPLSLLSQQNKFLKDMGISGVGFTPAFIVLVIYALFPVVHNTIA
                     GLKMIDYELLETAAGLGMKNNTIFWKIQLPLSLPIILGGIRIAITQSIGNTILAGLIG
                     GGGLGSIIFLGLAQAAPDLILLGVIPLIAMAFLMDSVISFLIFYYKKRYLGLAE"
     gene            complement(3118..>3520)
                     /locus_tag="PKJ95_02905"
     CDS             complement(3118..>3520)
                     /locus_tag="PKJ95_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNR65227.1"
                     /translation="GLRGFEEAYGFHLSADQIIALASGNTAEMLKALVEMTNGVNVSL
                     VYGTDGALDKMDLVVLDDPKSIPPVYLPAPVLQGALYEKYPEIEKPLNRLFQSLNENN
                     LRQLNSKVAYDGMAAEAVAREYLEENELLIK"
BASE COUNT         1106 a          717 c          624 g         1073 t
ORIGIN      
        1 ggggcgtatc tgaccactgt gggagaagtt gccggagaga ggcattctgg tactgctact
       61 gggttagctc tattgtttgt caggggtgcc atgattgtat ctccgccaat ttttgggctg
      121 attgccgata taaatggttc ttatagatat agctggttaa tatatagttt tgttattatc
      181 ggtatgtctt ttcttttttt accactgaaa aaacagccag ttgttcttac ctaaaggtta
      241 ttctgatatt gctgataatc gaaatctctt ttctaaaata ataaaaagac tttctactgc
      301 aagagccagc agagaaaccg ggattgctcc tttcagaatc atgatggtat catcacctct
      361 aattccattt actatcagtg tgccaaatcc gctcagtccc acagtcgcac ctatggttgc
      421 cacactgata ttgagtatca aggatattct aattcctgct aaaattgccg gaagggcaag
      481 tggtaactca actctggtga taatctgaaa tctattcatt cccattgccc gagcagattc
      541 tcttatctcc tcaggtatag tattaaatcc ctcaatggta tttctcaata ccggtaagaa
      601 cccatataca aatagagcaa agagggccgg tttgaagcca tatcctaaga aaggaaccaa
      661 aagggcaagt atggcaactg tgggaatggt ctgtccgaag gtggccagcc ctaataagaa
      721 ttcttcaaaa tcactcatga aggggaaccg ggttaagata gcgatggtaa gtgcaataat
      781 gattgataga gaactggaaa tgattacaat aaagatatgt tgtttagcaa tttcgactaa
      841 acttactctg gctagaaaaa aactttttgg ttttacaaga aggacaagaa cattctcaat
      901 gatatcaaat cttaaaatac tgataagaaa gaaggcaaaa agtagggcag taacaataaa
      961 aaatggttgt tttatttttt tcattttctt gtatctctaa aaacattctt tagaatatct
     1021 tcaaaggtta tagagccgac aatcttctct tgctcatcta tgacttctaa agaatcaatt
     1081 cccttagaaa tcattaaaga gagcgcactg gaaagggtat tttccttttt aacctgaaaa
     1141 ccatgtatat agcctttact cttaatcttt tcttgcacca gtatatcctg taccataaac
     1201 ctggttaaaa gcttcagggt atattcactt cccaaaaatt cttttacata atcttcttta
     1261 tcgtctgtaa caaaatccca tggacttccc agagaggcca attgacctgg tttcatcact
     1321 gctatattat cagccagtct gatagcctct tccacatcat gtgtcacaaa tagaacagtt
     1381 tttttgagtt ctctttgaat tctgtaaaat tctttttgta atctctctct attcagagga
     1441 tctactgaac caaagggctc atccatgagc aatataggag ggtctgctgc cagtgccctt
     1501 gccacaccaa ccctctgtcc ctctcctcca gatagttcga aagggtattt cttcttataa
     1561 tttctattta atcctaccag gtccagaaga taatcaaccc gccggtcgat atcatttttt
     1621 gtccatttga gcagccgtgg aacgataaca atattttcat agacattata atgggggaaa
     1681 agcccaatac tttgaataac ataacctata cctctcctga gattgataag attgaactca
     1741 tttatattct tattatttat atatatctcc cctgaggtag gttcaatgag gtgattgata
     1801 agttttatta cggttgattt tccgcagcca gatggaccca gcagtacctg tagaattcct
     1861 tcaggaattt gtaaactaaa attcttcaaa gcagtgatat cattatactt tttggttaca
     1921 ttcttaagct ctatcattct gctaacccca aatacctctt cttataataa aagataagaa
     1981 atgaaattac tgaatccata agaaaagcca tggcaatgag agggataaca cccaacaata
     2041 tcaagtccgg agcagcctgg gctaaaccca ggaagataat actgcccaac cctccacccc
     2101 cgataagccc ggctaaaata gtattgccaa tagactgagt tatcgctatt ctgatacccc
     2161 ccaggataat tggcagggat agggggagct gtatcttcca gaaaattgta ttattcttca
     2221 tgcctaaacc ggctgctgtt tccagcagtt catagtcaat catttttaat ccggcaatgg
     2281 tgttatggac tacaggaaaa agagcatata tgaccagaac aataaaagcg ggagtaaatc
     2341 ccaccccgct tatgcccata tcttttaaaa atttgttctg ctgactgagt agtgaaagag
     2401 gtaccatgat tatgcccaac aatgataggg tagggatggt ctgccccaga ttaattagaa
     2461 aaagaattgt actcttcatt tttctctctt tataaataag tacacctatg ggaataccta
     2521 taattaaacc tgctataaca gaaaatagtg atagagaaaa atgtcgtcta atctgcctga
     2581 aaaaagtgtt acttctattg aaaaattctt tcatgataga aagattattt agatgaccgc
     2641 tgagcagaaa aacaaaaacc atcaaaaaaa gtatggtata aaaaaccctt ctgtataata
     2701 tacacttttt gtcacccttg aagcatccac tcattaccat ctccataccc aaaaaaatgg
     2761 cccagaaccc tattcctaaa gatattctac catttggaat tatattaaag gtttcttttt
     2821 ctatggctat tccaaataga tataagacta atcccattat aagaagtgag gttacagtaa
     2881 tgattagttt atgcccttta tggttggtaa tgattaggaa gataattagt atagagagaa
     2941 atatcagtaa gaaacaacca ctgccaaaaa gttccctggc accaatattg gttccactga
     3001 gtactctgtt gggtctatat tctgcaaaac tcagtaaaag aaaagaaaga attagaataa
     3061 atgctcccag aatagagatt ttctttccgg aatcaggcat atgaaaccct ttacccttta
     3121 cttgattaac aattcattct cctcaagata ttcccgggca accgcctctg ctgccatacc
     3181 atcataggct acctttgaat ttagctgtct caggttattt tcatttaggc tctggaataa
     3241 tctatttaac ggtttttcaa tttccggata tttttcatat aaggctccct gtagaacagg
     3301 ggcaggcagg tagaccgggg gaatgctttt agggtcatcc aataccacaa ggtccatctt
     3361 gtctaaggca ccatctgtgc catataccag ggatacatta acaccattgg tcatttccac
     3421 cagagctttg agcatttctg ccgtatttcc agaggcgagt gcaataatct gatctgcact
     3481 gagatgaaac ccgtatgcct cttcaaaccc tctcaaacct
//
LOCUS       DAOQWT010000103         3513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_27821_length_3513_cov_4.243493, whole genome shotgun sequence.
ACCESSION   DAOQWT010000103 DAOQWT010000000
VERSION     DAOQWT010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27821_length_3513_cov_4.243493"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKJ95_02910"
     CDS             complement(<1..361)
                     /locus_tag="PKJ95_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNR65228.1"
                     /translation="MRVWVKIMQISKTVLIMAVVLLLTSCSGIIPETDLNIQDSCLDN
                     GIITENEYTARGSSVCLDVPYQKYAGVNWCLPASAAMTLDFLGNPISQQELAKKIIKP
                     DGLGDVYKMVRFAKELGY"
     regulatory      complement(418..504)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      complement(558..644)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(817..1959)
                     /locus_tag="PKJ95_02915"
     CDS             complement(817..1959)
                     /locus_tag="PKJ95_02915"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNR65229.1"
                     /translation="MTEQKRYRVGILFGGKSGEHEVSFCSASSVIKAINPGKYEAVPI
                     AITKQGYWLSPQNSHHALLTGKVEGEEIMGWKSKTDEHQFFIINKKNNEINYSFLEKL
                     DVIFPVLHGPFGEDGTIQGLLELINIPYVGSGVAASSLSMDKELMKKVFQQAGLPLTK
                     WLMFKRKRWVTEPKEIIREVEKKLCYPLFVKPTNLGSSVGVSKVKKSNELSAAIDMAA
                     LYDRKILIEEGVEDNIEVECSVLGNDEPLVSVVGEVKPAGPYYDYNSKYLDDNTQLII
                     PARIPDRVAKEIQEISRLAFLAIDAAGLARVDFLVQQKGNSYHIFLNEINTIPGFTEV
                     SMYPKLWEKSGVGFSELIDKLILLALERYQDKLSNKIDYPSKLLEK"
     gene            complement(1961..3310)
                     /locus_tag="PKJ95_02920"
     CDS             complement(1961..3310)
                     /locus_tag="PKJ95_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNR65230.1"
                     /translation="MDLFQQQVNKIAGLAPLADRMRPKSLEEFSGQEKIIGKGKPLRI
                     AIENDQLHSIIFWGPPGSGKTTLAMIIAHLTSSFFVQFSAVTSGIPDLKEMIKEIKQR
                     WTFNQQKTILFVDEIHRFNKTQQNAFLPYVEDGTIILIGATTENPSFEVISPLLSRSN
                     VYVLDSLSGQDLSKIIERALSDLDNGYGKLPINISKEVINLIIQLSYGDARVALNILE
                     FAVNTGIASIKNSNKISIDIDKELILEIVQKNILRYDKDGEEHYNLISALHKSMRDSD
                     PDASLYWVTRMIEAGEDSLYIARRLIRFASEDVGNADPRALSVAVSAMQAVHFLGMPE
                     GDLALVQAALYLANAPKSNSLYKGRKLAGQDIKRYGALPVPLVIRNAPTRMMKDMGYG
                     AGYQYAHDFQNALVEQQHLPEQLKDRKYYFPSERGLEKKIKEKMEQREKMKKSLRKE"
BASE COUNT         1051 a          726 c          548 g         1188 t
ORIGIN      
        1 tgtagcctaa ttctttagcg aatctaacca ttttataaac atcacccaat ccgtctggtt
       61 tgatgatttt tttggctaat tcttgctggc tgatgggatt ccccagaaaa tccaatgtca
      121 tagcggctga agcaggcaga caccaattaa cccccgcata tttttgatat ggtacatcca
      181 gacaaactga actacctctg gctgtatatt cgttttctgt tatgatacca ttgtcaagac
      241 aactatcctg aatgttcaaa tctgtttccg gaattattcc actgcaggaa gtcaataata
      301 atacgactgc catgataagc acagttttac tgatttgcat tattttcacc caaactctca
      361 taccccactc ctttataatc agttgaaagg cagactgagt ttgggctaaa gaaaaaaggt
      421 ggcagtctgg cagccatagt cttttttgac tttaggccca aaactttgcg tcctattctt
      481 tcgaatagtt tgcctttttc aacctctatt tattaatctt attttgttca aaacaattat
      541 gataaaaaga aaaagaagtc ggcagtctgg caatcttagc ggtttaccgc cttagaccca
      601 gtgctttgcg tcctattctt tcgaatagtt tgcctttttc aacctctatt tacagtttat
      661 cgattgtcaa atagcaacaa tgggatttac ttcccgtaaa aatctcttaa attttcaacg
      721 ataagaaaga actttaacaa atattattct tttctactat aattattccc taaaaaattt
      781 tttttagaca ataattttaa aaaaaataaa aaatatttat ttttctagta attttgaagg
      841 ataatctatt ttatttgata atttatcctg ataacgttcc agagcaagca gaatcaattt
      901 atctatcaat tcactgaaac caacaccact cttttcccat aatttggggt acatactgac
      961 ttcagtaaaa cctggaatag tattaatctc atttaaaaag atgtgataag aattcccttt
     1021 ttgttgcacc aaaaaatcaa ctctggctaa accagcagca tctatggcta aaaaagccag
     1081 ccgggagatt tcctgaattt cttttgccac tctatctggt attctagctg gaataattaa
     1141 ttgggtatta tcatcaagat atttagaatt ataatcataa taaggaccgg ctggtttaac
     1201 ttctcctact acagatacta ggggctcatc attacccaaa acactgcatt ccacctcaat
     1261 attgtcttca actccttctt ctattagaat ttttcggtca tagagcgcag ccatatctat
     1321 ggcagcagat aattcattgc ttttttttac tttactgacc cctacactgg accctaaatt
     1381 tgttggtttg acaaaaagag ggtaacacaa ttttttttct acctccctta ttatttcttt
     1441 cggttcagtg acccatcttt ttcttttgaa catcaaccat ttggttagag gaagaccggc
     1501 ttgctgaaaa acttttttca tcagctcttt atccattgat agggaagagg cagctacccc
     1561 tgaacccaca taagggatat ttattaattc caataatcct tgaatagtgc catcctctcc
     1621 aaatggtcca tgaagtactg ggaagataac atctaatttt tctaaaaaag aatagttaat
     1681 ttcattgttc tttttgttaa taatgaaaaa ttgatgttca tctgttttac ttttccagcc
     1741 cataatctct tccccttcca ctttcccggt aagcaaagca tgatgactat tctggggtga
     1801 taaccaatag ccctgtttag tgatagcaat tggtaccgct tcgtattttc ccggattaat
     1861 ggctttaata actgaagatg ctgaacagaa agaaacttca tgctctccgg atttaccacc
     1921 aaacaatatt cctactctat atcttttttg ctctgtcatt ttattccttc cttaaggatt
     1981 tcttcatttt ttctctctgt tccatttttt cttttatctt tttctccaat cccctctcag
     2041 aaggaaaata atattttcta tctttcagtt gttctggaag gtgctgttgc tccaccaaag
     2101 cattttgaaa atcatgggca tattgatatc cggcaccata acccatatct ttcatcatcc
     2161 tggttggggc attacgaata accaaaggta ccggcagagc cccgtatctt tttatatcct
     2221 gtccagcaag ttttcgacct ttgtaaagag aattactttt aggagcattg gctaaataaa
     2281 gagcagcctg caccagtgct aaatctcctt ctggcatacc cagaaaatga actgcctgca
     2341 ttgctgaaac tgcgacagaa agggcccggg gatctgcatt tccaacatct tctgaggcaa
     2401 atctgattaa tctccgggca atataaaggg agtcctcacc agcttcaatc attcgggtaa
     2461 cccaatacag actggcatcc ggatcagaat cgcgcatact cttatgtaag gctgagatga
     2521 gattataatg ttcttcccca tctttatcat aacgtaaaat attcttttgt acaatttcca
     2581 aaattagctc cttatctata tctatgctta tcttattgct gtttttaata ctggcaatcc
     2641 cggtattaac agcaaattcc aggatgttca aagccacccg ggcatctcca taggaaagct
     2701 gaataatcag attaatcact tctttactaa tattaatggg tagtttccca taaccattat
     2761 ccaggtcact taaagcgcgt tcaatgattt tggataaatc ctgcccagag agggaatcga
     2821 gcacatagac attagaacgt gacaatagag gagaaattac ttcaaaagaa ggattttcag
     2881 tagtggctcc aatgagtata atagtgccat cctcaacata gggaaggaat gcgttttgct
     2941 gcgttttgtt gaaacgatgg atctcatcaa caaagagaat ggttttctgt tgattaaaag
     3001 tccaacgctg tttgatctcc ttaatcatct ctttcaaatc aggaatccca gaggtaacag
     3061 cactgaactg aacaaaaaaa ctgctggtca aatgggcaat aatcatagcc agggtagtct
     3121 taccagatcc aggaggaccc caaaaaataa tggaatgcag ctgatcattt tctatagcaa
     3181 ttcgtagagg ttttccctta cctataatct tctcctgtcc agaaaattct tccagacttt
     3241 ttggcctcat tcgatcagcc aaaggagcta atccggctat tttgtttacc tgctgttgaa
     3301 ataggtccat ttttctaact ttaatcctat ttaaagtctt ttttattatc ttatcatttt
     3361 tttactactt ttaaaacaag ataataagca caaaaagttt cattttacag tctacacaat
     3421 tatttctaaa atattcgaaa aaatataaag atagtctctt tttttgtgat attatgaagt
     3481 atattattac cttctgttat ttatttagcc att
//
LOCUS       DAOQWT010000104         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_27846_length_3510_cov_4.686831, whole genome shotgun sequence.
ACCESSION   DAOQWT010000104 DAOQWT010000000
VERSION     DAOQWT010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27846_length_3510_cov_4.686831"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PKJ95_02925"
     CDS             complement(<1..618)
                     /locus_tag="PKJ95_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65231.1"
                     /translation="MLQLFNHVDLTEASKRKPQDIFPGDNTTQAFLFYNLNPDILARV
                     FLNDFFQMMLTDFYTYWVYPIGTSCLFFYSPPLIGTGFLGTGLIGTNNITCQSNECLF
                     FPGGTSDYGYLNACEGDSIDLPYYLSNDDYLAFYSDVTISSMCFLDSEDRLEVKLALT
                     QAGLDSAVAGEPLLIGDIIDSDLPHEFWIRLTVPRDYIGPNMSLFN"
     gene            complement(618..1226)
                     /locus_tag="PKJ95_02930"
     CDS             complement(618..1226)
                     /locus_tag="PKJ95_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65232.1"
                     /translation="MINLKVLDTIPEEITEVDEGSTFYPYSKETKLTLKNEGTYKARN
                     VTVSISKGVTVQNYSSPAMIAEAKIEAHGYQFAVEYFDLITPFNNTIADLANFPLLQK
                     EVVIDQDGYCRNIINGMAISFNHLLQYHNSGIYISEAYYMCGVALDINGVAGDYTNMV
                     KFPEIEVNEEVSFWYRFFLRRDMQAQRNPRVFNLVIKGEEDR"
     gene            complement(1223..1648)
                     /locus_tag="PKJ95_02935"
     CDS             complement(1223..1648)
                     /locus_tag="PKJ95_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65233.1"
                     /translation="MEWTQISCYSDSFSFTDQGSTYKFKCDKSGTDVPYNPPDIIDQA
                     PPYRGSSKPTIGGNRVEQYFGSYVCDGEIGFSLYLSKEDVDDFKAAFALGATWTLRDY
                     TGGGYIVSFEFNDGFKVGQKLQANDKYWCTFKFRKHATA"
     gene            complement(1650..>3510)
                     /locus_tag="PKJ95_02940"
     CDS             complement(1650..>3510)
                     /locus_tag="PKJ95_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65234.1"
                     /translation="PGLSKEAKEQWKLKEDQEARLNMVNATLREKAQNKQNIRSKLAA
                     NTPGKGEVDTPEMMLNRAYYQKQLNAALDDYEKHLIMRQNLTSGFGKEDMDLQIKQKK
                     EFDTLFMNEDTKKREAIKENYRQLMQQYEGDAQKQAQIQQQLNKELGELAVEQADKAI
                     KEKQRVLDEIKKLKEEELKHAQTKAEQLLEIDKQKNLAMVKQAGLAEIEKLKASGKAT
                     PEAVKKIQDRVEAATKAMTGKYDVKSEELKQTQAQQDINLKQDEFKDLQKRYAEEKKR
                     YEQADKALKGLKTPPGETRKRSEWEIETPEYKRREEAKSNANDAVRQMQKLSEEIGQL
                     NINIAESDTKIKDIKYQKDIDYLTETDLEKKKIMKNAEATYGIGSSSRSTPSSVYSGN
                     LMTRYKEWQEWGNKGSFADYLKTLSEPAPAIQPSVKPGTEGGKGASSSVFSMGSGGGS
                     SSVSLVNSVTKFNDKENPYMKEEISLERLKGLRQSRYDQLVKEFNLISKIADRQKFGA
                     DKELGSFAGVGKVPGAIGQSFARILANQVPIPNIGGDIFSKDKSLGQINAAIKAKEQG
                     GVNPSTTEAIGTTINVNIGDASSQAKLPPHIEDNLAKTGKEVIQYMENLGVLK"
BASE COUNT          909 a          774 c          578 g         1249 t
ORIGIN      
        1 attaaacaga gacatatttg ggcctatata atccctggga acagtaagcc ttatccagaa
       61 ctcatggggt aagtcgctgt ctataatatc acctataaga agaggctcgc ctgctacggc
      121 tgaatctaat cctgcctgag taagtgcaag cttaacttcc aatctgtcct ctgagtccag
      181 aaaacacata gaagatattg taacgtcgct ataaaaagca aggtaatcat catttgaaag
      241 ataataaggt aagtctatac tgtcaccttc acaggcgttt aaatacccat agtctgaggt
      301 tcctcccgga aaaaacagac attcattact ctgacaggtt atgttatttg ttccgataag
      361 tcctgtgcct aaaaaccccg ttccaattaa aggaggggaa taaaagaaaa ggcaggaagt
      421 tccaatagga tatacccaat aagtataaaa atcagttaac atcatctgaa aaaaatcatt
      481 taaaaacact cttgccagta tatccgggtt aagattatag aataaaaaag cctgtgttgt
      541 attatcgcca gggaatatat cctgcggttt tcttttactt gcctctgtta aatctacatg
      601 gttaaacaac tgtaacacta tctgtcttct tctcctttaa ttaccaggtt aaaaactctg
      661 gggtttctct gtgcctgcat atctcttcta aggaaaaatc tgtaccagaa agaaacttct
      721 tcattaactt caatctccgg gaatttcacc atattcgtgt aatctcctgc tacgccgttt
      781 atatccagtg ccacaccaca catataataa gcttctgata tatagatgcc ggagttatga
      841 tattgaagca aatgattgaa agaaattgcc ataccgttaa ttatattcct gcagtatccg
      901 tcttgatcta ttacaacctc tttctgaagt aatgggaaat tggcaaggtc cgctatggta
      961 ttattgaaag gtgtaattaa atcaaaatat tcaactgcga attgataacc atgagcttct
     1021 atcttcgcct ctgctatcat ggcaggagaa gaataattct gcaccgttac gcctttagag
     1081 atactaactg ttacattccg ggctttatat gttccctcgt ttttcaatgt aagtttagtc
     1141 tcttttgaat aagggtaaaa ggtgctgcct tcgtcaacct ctgttatttc ttctggtatt
     1201 gtatctaata ctttaagatt aatcatgccg tcgcgtgctt tctaaattta aaagtacacc
     1261 agtatttatc atttgcctgg agtttctgcc ctactttaaa cccatcgttg aactcaaaag
     1321 atacaatata tcctcctccg gtataatctc ttaatgtcca tgtcgcaccg agagcaaagg
     1381 ctgctttgaa atcgtcaaca tcttcttttg aaagataaag tgaaaatcca atttcaccgt
     1441 cacagacata agatccaaaa tactgttcaa ccctgtttcc tcctatagtc ggcttacttg
     1501 aacccctgta aggaggagcc tggtcgatta tatcaggagg gttataagga acatctgtcc
     1561 cagatttatc gcatttgaat ttataagttg aaccctggtc agtaaaagaa aaactatctg
     1621 agtaacagga aatctgtgtc cattccatat tattttaaca ctcctaaatt ttccatatat
     1681 tgtattactt ctttcccggt ctttgccaga ttatcctcta tgtgaggagg taattttgcc
     1741 tgacttgaag catcgccgat attaacatta attgtcgtgc ctatggcttc ggtagtcgaa
     1801 ggatttactc ctccctgttc ctttgccttt atagcagcgt ttatctgtcc gagtgactta
     1861 tcttttgaga atatatctcc tcctatattt ggaataggaa cttgattggc taaaatcctc
     1921 gcaaaagact gaccgattgc gccgggaacc ttacccacgc ctgcaaaact accaagttct
     1981 ttatctgccc cgaacttctg ccggtctgct attttagata taagattaaa ttcttttaca
     2041 agctgatcat atctactttg ccttaaccct tttaatctct ccagggagat ctcttctttc
     2101 atatacgggt tctctttatc gttaaatttt gttactgaat tgacaagaga tacagaagaa
     2161 gatcctcctc ctgaacccat actaaataca gaagaacttg cacctttacc gccttctgtt
     2221 ccgggcttca cggatggctg aattgccggt gcaggttcag acagagtttt aagataatct
     2281 gcaaaacttc ctttattacc ccactcctgc cactctttat atctggtcat aagattaccg
     2341 ctatatactg aagatggggt ggatcgagaa gaactgccta ttccataagt tgcttctgca
     2401 tttttcatta ttttcttttt ttcaaggtct gtctctgtta aataatctat atctttctgg
     2461 tattttatat ctttgatttt tgtgtcagat tccgctatat taatatttaa ctgacctatc
     2521 tcttctgata gtttctgcat ctggcgtact gcatcatttg cattactctt tgcttcttct
     2581 cttctcttat actcaggggt ttcaatctcc cactctgacc tttttctcgt ttctcccgga
     2641 ggagtcttta atcctttcaa tgccttatct gcctgctcat atctcttctt ttcttcggca
     2701 tatctctttt gtaaatcttt aaattcatcc tgctttaaat taatatcctg ctgtgcctga
     2761 gtttgtttta attcctcact ctttacatca tacttaccgg tcatggcttt tgttgctgcc
     2821 tcaaccctgt cctgtatctt ttttactgct tcaggagtag ctttgccgga ggctttcagt
     2881 ttttctatct ctgcaagccc tgcctgcttc accattgcaa ggtttttctg tttatctatt
     2941 tcaagtaact gctctgcctt tgtttgagca tgttttaatt cttcttcttt aagcttttta
     3001 atttcatcta aaaccctctg cttttcttta atagccttgt ctgcctgctc aactgccagt
     3061 tctccgagtt ctttatttaa ctgctgttga atttgtgcct gcttctgagc gtcaccttca
     3121 tactgctgca taagctgccg atagttttct ttaatagcct cacgtttttt ggtatcttcg
     3181 ttcataaaca gggtgtcgaa ttcttttttc tgttttattt gaagatccat atcttccttg
     3241 ccaaatcctg aagtaagatt ttgcctcata attaaatgtt tttcataatc atcaagggct
     3301 gcattaagct gtttctggta atatgccctg tttaacatca tctcaggtgt atcaacttcg
     3361 cctttacccg gagtatttgc agctaattta cttcttatat tctgtttatt ctgtgccttc
     3421 tcccggagag tggcgttaac catgtttaat cgggcttctt gatcttcttt taacttccat
     3481 tgttcctttg cttctttact cagtccaggc
//
LOCUS       DAOQWT010000105         3493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_28056_length_3493_cov_2.642234, whole genome shotgun sequence.
ACCESSION   DAOQWT010000105 DAOQWT010000000
VERSION     DAOQWT010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28056_length_3493_cov_2.642234"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            471..989
                     /locus_tag="PKJ95_02945"
     CDS             471..989
                     /locus_tag="PKJ95_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNR65235.1"
                     /translation="MNNAKKVALLAENLYEDIELWYPYYRLKEAGYEVQIVAPEKKTY
                     YSKHQYPVQADLSIEGAKTDDFLGVVIPGGYAPDYLRRSSRILNFVRKIFEKNGLVAT
                     ICHAVWVPISAGILNEKDATCVFAIKDDLINAGANYLDQEVVVDHNLVSSRTPKDLPA
                     FLPAILKVLDEL"
     gene            1045..1719
                     /locus_tag="PKJ95_02950"
     CDS             1045..1719
                     /locus_tag="PKJ95_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65236.1"
                     /translation="MRKILFTILPYGVLCLLTIIIIILFWPMEQGGLVELTNSWIGLF
                     FVYAFCAIFLFQDDIYHFLEQNIKSNFLLSPTQLQKDQVIPEIFIDDEQFQEIISGTL
                     MGWMDKLNIEKEEKRLKVEDIAQTVERYKKEKKENLKWLFLFTDYFLVPHSKDVLYEI
                     YERHYINENVLKEISIDLSLDDIETEAILEALTFLKFIRKQEEEFIITETGSAYCSYL
                     ERINQK"
     gene            1996..2946
                     /locus_tag="PKJ95_02955"
     CDS             1996..2946
                     /locus_tag="PKJ95_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNR65237.1"
                     /translation="MKYNKLGNSGIKVSELCFGVLPMGPLQANISVKDGAKIIRKGLN
                     SGINFLDTAQAYKTYPYIKEALTGYKEDVVIASKSYASSYDDMKEAVLEACREMGRDY
                     IDIFHLHAAREDGSVFQKRAAALSALVDLKKEGLIRAIGISTHAIEVVEESAEISDID
                     IVFPIINQIGLGIIGGTTEEMIRAIKKVSVRGKGIYAMKALAGGYLITQLLESLNFVR
                     NIKEIQSTAIGMVHPRELEINLKIFENKKIEQEELVKEGFIRKRLFISFFCEGCGTCV
                     EACPNQALSLKEGKAVVDYELCLTCGYCVPHCPVFALRIV"
     gene            complement(2978..>3493)
                     /locus_tag="PKJ95_02960"
     CDS             complement(2978..>3493)
                     /locus_tag="PKJ95_02960"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65238.1"
                     /translation="TYFANVCGIGFDADIAALANQMKTRYPHWKTLSAYVYVFATIQK
                     LLSSLPSFQAKITIDGHVLEKELLLVAIANGRIEGGKFNITPKAEIDDGLLDICIIEK
                     MNRFRCLYLLPKAIKGTHHEVSEVSFFKGKDILIELNKSVTAQVAGEILPPEKKYHIR
                     VLPEKLKLIIP"
BASE COUNT         1187 a          513 c          681 g         1112 t
ORIGIN      
        1 tattaggtgg agaaaatact tatgaagact tattgaatat ttttgaacaa gcgggattag
       61 aattatataa tgagattgat aaaaaaatta gaacactaaa gtaaatagct gaacacacca
      121 tgctataccc aacagaggtt tattttctag taattgaaaa ttagtaagat aaattaggtt
      181 ctagcatcaa aaaagtggat cctattatat ctaaaagcta caaaaaatat gctacgataa
      241 taaaaatcac tcaaagatgg agatataatt tattatattt ccaaaattcc cttaaaatga
      301 caatataaac tgatatatga gttaaggctt accgagaaat tgagagaaat atgatggcta
      361 attgcttctt attagtttta atttatggta ttattatttt gaatataata tgatgaaata
      421 tttgaaacaa taggtgcttt agaatataag aatgttggag gggaataaaa atgaataatg
      481 ctaagaaagt tgctctgctg gcagagaatt tatatgaaga tattgaatta tggtatcctt
      541 attacagact taaagaggca ggatatgagg ttcaaattgt ggcacccgag aagaagactt
      601 attacagtaa acaccagtat ccggtccagg cagacttatc cattgaagga gcaaagacag
      661 atgatttcct gggagttgtt atcccgggtg gctatgctcc tgattacctg cgaagatctt
      721 ccaggatttt gaattttgtc cgtaaaatat ttgaaaaaaa cggtctggtt gccacaattt
      781 gtcatgcagt ctgggttcct atttcagcag gaatattaaa tgaaaaagac gccacttgcg
      841 tttttgccat aaaggatgat ctgataaatg caggagctaa ttatttagat caggaagtag
      901 ttgttgatca taacctggta agttcccgta cacctaaaga tttaccggct tttcttcccg
      961 ctatactaaa agtattagat gaattataat taatatggta aaaaataaaa gaataattaa
     1021 tcatttttaa aagtgggaaa ccagatgcgt aaaattttgt ttactatttt accttatggt
     1081 gttttatgcc tgcttaccat aataataatt atactctttt ggcctatgga acagggaggg
     1141 ttagtagaat taaccaattc ctggattggc ttattttttg tttacgcatt ttgcgcgatt
     1201 tttttattcc aggacgatat ttaccatttt ttagagcaga acatcaaaag taatttttta
     1261 ctttctccga cacaattgca aaaagaccag gtaattccag aaatatttat agatgatgag
     1321 caatttcagg agataatttc aggtaccttg atgggctgga tggataaact gaatattgaa
     1381 aaagaagaaa aaagattaaa ggtggaagat attgctcaaa ctgtggaaag atacaaaaaa
     1441 gaaaagaaag aaaatctaaa atggttattt ctttttaccg attatttttt ggttccacat
     1501 agcaaagatg tcttatatga aatatatgaa agacactata taaatgaaaa tgtgttgaag
     1561 gagatatcca tagatttatc ccttgatgat atagaaactg aggcaatttt agaagcttta
     1621 acatttttaa aatttatcag gaaacaggag gaagaattta ttattactga aactggctcc
     1681 gcctattgtt cctacctgga gaggattaat caaaaataag agagatagtg gatagtgctg
     1741 tctcaatttc aagtagtata cattccttca atgattggat agtaataatt ttccagtacc
     1801 actatttatc caaccaaccg ctctggaaac ttcaattatc taattttaat cgcttcattt
     1861 ttatttcttt tttcattatc atgatagcct tataagttta aatagattta aatactttat
     1921 tgaaaattga atagatggta tgctattatg ctgattcaat ggagagaata aagcaggaga
     1981 ggacaggaga aaaagatgaa atataataaa cttggcaatt caggaataaa agtaagtgaa
     2041 ctgtgttttg gtgttttacc tatggggcca ttacaagcca atatttccgt aaaagatggg
     2101 gctaaaatta tacgaaaagg attaaattcc ggcattaatt ttctggatac tgcacaggcc
     2161 tataagacct atccttatat taaagaagcc ctgaccggat ataaagaaga tgtagttatt
     2221 gccagcaaat catatgcttc tagctacgat gatatgaaag aggctgtttt ggaagcctgt
     2281 cgggagatgg gacgtgatta tattgatatt tttcacctcc atgcagctcg agaagacggg
     2341 agtgtttttc agaaaagagc agctgctcta tctgctctgg tagatttaaa aaaggaagga
     2401 ctgattagag caattgggat ttctacccat gccattgaag tagtagaaga atctgccgaa
     2461 ataagtgata ttgatattgt ttttcccata attaaccaga ttgggctggg aattatcggg
     2521 ggaacaacag aggaaatgat tcgagcaata aaaaaagttt ctgttcgtgg taaaggtatc
     2581 tatgccatga aagctttggc tggtggctat cttattacac aattattgga atcccttaat
     2641 tttgtcagga atattaaaga gatacaatca actgccattg gtatggttca tcctcgagaa
     2701 ttagagataa atcttaaaat atttgaaaat aaaaaaatcg aacaagaaga attagtcaaa
     2761 gagggattta tcagaaagag actattcatt tctttctttt gcgaggggtg tggcacctgt
     2821 gttgaagcct gccccaatca agcactgtcc ctgaaagagg gtaaagctgt ggtagactac
     2881 gagttatgcc tcacctgtgg ttactgtgta ccacattgtc cagtgttcgc actgagaatt
     2941 gtgtagcaga atgttgctta atgaattttg ttaagaatta gggtattatc aatttaagct
     3001 tttcaggtaa gaccctgatg tgatatttct tttcaggtgg taatatttca ccggcaacct
     3061 gtgccgttac agatttattt agctctatca gtatatcttt tcctttgaaa aaacttacct
     3121 ctgatacctc atggtgtgtt ccctttatag cttttggcaa taggtaaagg caacggaatc
     3181 tgttcatctt ttcaataatg cagatatcca ataatccatc atcaatttcg gctttggggg
     3241 taatattgaa tttaccgcct tcaattctgc cattggcaat tgctaccaat agcagctctt
     3301 tttctaatac atgtccatca atagttattt tggcctgaaa agagggcagg gaggagagca
     3361 gtttttggat ggtggcaaag acataaacat aagcactcaa tgttttccag tggggatacc
     3421 tagttttcat ctgattggct aaagcagcaa tatcagcatc gaaacctatt ccacagacat
     3481 tagcaaaata tgt
//
LOCUS       DAOQWT010000106         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_28151_length_3485_cov_3.772012, whole genome shotgun sequence.
ACCESSION   DAOQWT010000106 DAOQWT010000000
VERSION     DAOQWT010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28151_length_3485_cov_3.772012"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="PKJ95_02965"
     CDS             complement(<1..1237)
                     /locus_tag="PKJ95_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNR65239.1"
                     /translation="MNSDNEITLLDREARKQIMEDFEHNFLVEASAGSGKTSCLVKRM
                     AALIKSGRYTVEHIAAITFTRKAAIELKERFQQEIERELQQTDNPEEKNFLFQAITNI
                     EQCYIGTIHSFCARILRERSIEAGLDPAFKELDEVDNILLMEQAWEHYLSNLKIEDAA
                     RFKNLATLGIQIQDLKESYKKLCQYPEVEPIYKEVVSPQLDSALSKLYSFCDEAKQFI
                     PEEEPEGGYDTIQQAILQVHKLKSYPPYLEKDFYKVILLELFNRDSVKLGEIVLKRWL
                     SKEKAKEYRDRLLPELKTECIVPAMERWWEYCHSHTINLIKPAVDYFEQLKGKNSLLN
                     FQDLLLKAATLLRDNPEIRRYFQQKYQTILVDEFQDTDPIQAEIIFYLTGEQLEEDDW
                     RKLVPRTGSLFIVGDPQQSI"
     gene            complement(1230..>3485)
                     /locus_tag="PKJ95_02970"
     CDS             complement(1230..>3485)
                     /locus_tag="PKJ95_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNR65240.1"
                     /translation="YPFDKVQVFYTTQEPYSQYFYQLSLLYQIPMTFHSGISIKNSCP
                     AQFLFSLFDWIIDNYSIAKLITLLKYSSLDRNIRSTETEQKFTSLLRQSPIGWGRERY
                     LPGFDLAIEEREKQINNTSKEKAKELSDDIQHLFALKEWIEELFKEIPDQKFEHLISL
                     SRLAGGLLRLVKKYAQKEKGNIDEEAELIIEHKLDALQIDISTQITINEGLLLIKNLI
                     EQERINCSTPLPGHLHIASYKKGIWIDRLYTFLVGMDYQKFPGSSNGETLLLETEMHP
                     FQHLLNNNQKNKIEQLRLLQLILSRREKICLSFSGYHTTVQREQSPANLFFQLFRFQK
                     KNINLDYSIFYQDLSPARSFIPENSIEILDEGEFFLYFAKQEKMDLQSLYAQKYITFR
                     EGIRADRIRTEEGFNAYNGNIQVNANITDPRKNRGIVLSASKLERIAYCPYLYFLLDI
                     LKIKPPKEMAYDPFTWLDPLERGLLLHKIFERFYRILLSNSNGRKVIPSYSQHWNLLK
                     ETAEESLAERRRHLAPPGELVYEAEKKEILESCQIFLKEEEEIYKRYSNRPLYFELAF
                     GTRDNEHEILGKIKAIELKIPDGNRISIQGKIDRVDVLPDGTFLVIDYKTGSSRDYNK
                     KNPFRHGQQIQHALYAMAVEDILAKKEVGYELKVSESGYYFPTTSGLGNIVLYQQHNR
                     EQVLEIINILLDLVTKVNFAMIQKAEELMCKDYKEIMEQNQVIPVKGTKGEIYDSEPA
                     LEEIRRLQQFE"
BASE COUNT          995 a          699 c          598 g         1193 t
ORIGIN      
        1 agattgattg ttgggggtca ccaacaataa acaggctgcc ggtacgggga actaacttcc
       61 tccagtcatc ctcttctaat tgttcaccgg ttaggtaaaa gataatttca gcctgaatag
      121 ggtctgtatc ctgaaattca tcaacaagta tggtctggta tttctgctga aaatagcgac
      181 gaatttcggg gttatcacgt aaaagagtag cagccttcag taataaatcc tgaaagttaa
      241 gcagagagtt ttttccttta agctgttcaa agtaatctac cgcaggttta attaaattga
      301 tggtatggct atgacaatat tcccaccatc tttccatagc cggaacaata cattctgtct
      361 ttaactccgg caacaatcta tcccggtatt cctttgcttt ttcttttgat agccacctct
      421 tcaatactat ttctcccaat ttaacagaat ctctgttaaa taattccaat aaaataactt
      481 tataaaaatc cttctccaga tatggtgggt agctttttaa tttatgcact tgaagtattg
      541 cctgctggat agtatcatat cccccttctg gctcctcctc tggaataaat tgttttgcct
      601 catcacaaaa tgaatagagc ttgcttaatg cagaatccag ttgagggcta actacttctt
      661 tataaattgg ttctacttcg ggatactgac aaagtttttt ataactctct ttaaggtctt
      721 gtatctgaat tcctaatgtt gccaaattct taaaccgggc agcatcctca atcttcaggt
      781 tagacagata gtgctcccag gcctgttcca ttaaaagaat attatcaact tcatccagct
      841 ctttaaaggc ggggtctaac ccggcttcta tggaacgttc tctaagtatt ctggcacaaa
      901 aagagtgtat ggttcctata tagcattgtt ctatgttggt tatggcttga aagagaaaat
      961 tcttttcttc aggattatcg gtttgttgaa gctctctttc tatctcttgt tgaaatcgtt
     1021 ctttaagctc aatagctgct ttacgggtaa aagtaattgc ggcaatatgt tccacggtgt
     1081 atctaccgga ttttatcagg gcagccattc ttttcactaa acaggatgtt ttgccagagc
     1141 cggcactggc ttcaaccaaa aaattgtgct caaaatcttc cattatttgt tttctggcct
     1201 cccggtcaag gagggttatc tcattatcac tattcaaatt gctgtaacct ccttatctct
     1261 tccagagctg gttcactgtc atatatctct ccttttgtcc ctttcacggg aattacctga
     1321 ttctgctcca ttatctcttt ataatcttta cacatcagct cctctgcttt ttgaatcata
     1381 gcaaaattta ctttggtcac taaatccagc aaaatattta ttatctccaa aacctgctct
     1441 ctgttatgtt gttggtacag gacaatattg cccagaccag aggtagtagg aaaataatat
     1501 cccgattcac tcactttcag ctcataccca acttccttct ttgccagtat atcttctaca
     1561 gccatagcat aaagggcatg ctgtatttgt tgaccatggc ggaaaggatt ttttttatta
     1621 taatctctgg agcttccagt cttataatcg attactagaa aagttccgtc aggtaaaaca
     1681 tctacccggt caattttccc ttgaatactg attctgtttc catcaggaat ttttaattca
     1741 atggctttaa tcttccctaa aatttcatgc tcattatccc gggtaccaaa ggccagctca
     1801 aagtacagtg gccgattaga gtacctttta taaatttctt cttcctcttt taaaaagatc
     1861 tggcaggact ccaatatctc ctttttttca gcctcgtaaa ccaattcccc cggtggtgcc
     1921 agatgccttc ttctttctgc taaactctct tcagccgttt ccttcaataa attccagtgt
     1981 tgtgaataag aaggtattac ttttctgcca ttagaattac tgagcaatat tcgataaaat
     2041 ctttcaaata tcttatgaag gagcagtcct ctctctaagg ggtcaagcca ggtgaagggg
     2101 tcataagcca tttctttggg aggttttatc ttaagaatat ctaataggaa ataaagatag
     2161 gggcaataag caattctttc cagtttagaa gctgataata ctatacctct gtttttgcgg
     2221 ggatcggtga tgttggcatt aacctgaata tttccattgt aggcattgaa tccctcttct
     2281 gttcttatcc tgtcagctct aatcccttcc cggaaggtaa tgtatttctg tgcatataat
     2341 gattgtaaat ccattttctc ttgtttggca aaatagagaa aaaactcccc ctcatctaaa
     2401 atttctatac tattttcagg aataaaactt ctggcagggc tcaaatcctg gtaaaaaata
     2461 ctatagtcca gattaatatt ttttttctga aaccgaaaaa gctgaaagaa cagattggca
     2521 ggtgattgtt cacgctgaac tgtagtatgg taaccggaga aagaaagaca tatcttttct
     2581 ctacgagata aaataagttg caatagacgt aactgttcta tcttattttt ttgattattg
     2641 tttaacaaat gctgaaaggg gtgcatttct gtttccaaca acaaagtttc tccattagaa
     2701 ctgccgggaa atttttgata atccattcct actaaaaagg tataaaggcg gtctatccag
     2761 attcctttct tatagctggc aatatgcaga tgaccgggta agggagtaga acagtttatg
     2821 cgttcctgtt caattaaatt tttaatcaga agtaggccct cattgatagt aatttgtgta
     2881 gaaatatcta tctgtaaagc gtctaattta tgctcaataa tcaactctgc ttcttcatct
     2941 atattaccct tttctttttg agcatatttt ttcaccaacc gtaatagtcc acctgctaat
     3001 ctggaaaggg aaatgaggtg ttcaaatttt tggtccggaa tttccttaaa taattcttct
     3061 atccattctt taagggcaaa taggtgttga atatcgtcag atagctcctt tgctttttcc
     3121 ttggaggtat tatttatttg tttttctctc tcttcaatag ctaagtcaaa gccagggaga
     3181 taacgctctc ttccccagcc aataggagac tgcctcaata atgaagtaaa tttttgctct
     3241 gtctcggtac tccttatatt tctatcaaga gaggaatact ttaatagtgt aattaacttc
     3301 gcaatactat aattatcaat aatccaatcg aataaagaga ataaaaattg agcgggacaa
     3361 ctatttttta tactgatacc actgtgaaaa gtcatcggta tctgataaag aagcgataat
     3421 tgataaaaat actgagagta tggttcctgt gtagtgtaaa aaacctgtac cttatcaaaa
     3481 ggata
//
LOCUS       DAOQWT010000107         3482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_28198_length_3482_cov_2.517946, whole genome shotgun sequence.
ACCESSION   DAOQWT010000107 DAOQWT010000000
VERSION     DAOQWT010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28198_length_3482_cov_2.517946"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..466
                     /locus_tag="PKJ95_02975"
     CDS             164..466
                     /locus_tag="PKJ95_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNR65241.1"
                     /translation="MDVNKIKPLGDRILVKPLQEEERTKGGIVLPDTVSKEKPQVAEV
                     LAVGPGRTNDDGKTFPMPFKKGNKVVYAKYSGTDIKGEDDEDYLLLSEKDVLALLE"
     gene            569..2203
                     /gene="groL"
                     /locus_tag="PKJ95_02980"
     CDS             569..2203
                     /gene="groL"
                     /locus_tag="PKJ95_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777516.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNR65242.1"
                     /translation="MAKQFLFEENARSALKKGADKLADSVKITLGPKGRNVVLEKKYG
                     SPTITNDGVTIAREIEVEDPFENMGAQFVKEVASKTNDIAGDGTTTATVLAQKILHEG
                     MRNVAAGANPMMLRRGIQKAVDKAVEEIKKLSIEIKDKESIANVASNSAAEREIGNII
                     ADAMEKVGKDGVITVEESKTMGTTLEVVEGMQFDKGYISPYMVTDAERMEAVLEEPLI
                     LITDAKISNMKGLLPVLEKVAQMGKPLFIIAEDVEGEALATLVVNKIRGTLNCVAVKA
                     PGFGDRRKEMLTDIATVTGGQVISEELGLKLENTDISMLGTAHQVKADKEETIIVGGK
                     GDVKEIKKREAQIRAQIEETTSDYDKEKLQERLGKLVGGVAIINVGAATETELKEKKH
                     RVEDALSATKAAVEEGIVAGGGAVLLNIIPALEKLKLEGDEQIGVNIILKALEEPTRQ
                     IVNNAGYEGSIIVEQLKHKEKGVGFDAEKGEYVDMVKRGIIDPTKVTRSALQNAASIA
                     GMVLTTECLIADKPEDKKEPVMPGGGGMPGGGMGMY"
     gene            complement(2337..3449)
                     /gene="ald"
                     /locus_tag="PKJ95_02985"
     CDS             complement(2337..3449)
                     /gene="ald"
                     /locus_tag="PKJ95_02985"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNR65243.1"
                     /translation="MIIGVPKEIKDKENRVAITPAGVTAFTKNGHQVFVQKAAGIGSG
                     IADQEYSDVGAIVLEEPAEIYEEADMIIKVKEPLPSEYSFIKEGQIVFTYFHFASSHE
                     LTQAMLDRKCISIAYETVETDDGDLPLLAPMSEVAGKTAGNLVAHYLSLPRGGRGVLL
                     GGVPGVKPAKVVVVGGGTVGTNAAKVASGMGASVVIFDINLKRLKYLDDIMPKNVVTL
                     ASNPFSIEEELKNADALIGAVLIPGAPAPKVVTEEMVKKMPKRSVILDVAIDQGGCIE
                     TSRVTSLSEPTYVLHDVLHYCVPNIPGAFPRTSTFALTNATMSYALEIANKGYKKAII
                     SNPAIAKGLNTIDGRVTYKPVADAFGYPYSSIEELN"
BASE COUNT         1218 a          560 c          740 g          964 t
ORIGIN      
        1 gagaataata caagaataag gctaatttga attaaaaatg gcttaaaaca acatttgcaa
       61 aatcaatatt cattttataa gatagtaatg gattttagca ctctctttat gagagtgcta
      121 aataatttta aaattaaaat atttagggag gaataaataa aatatggatg taaacaagat
      181 caaacccctg ggagaccgta ttttagtaaa accattgcag gaagaagaaa gaactaaagg
      241 aggaatcgtc ttaccagata cagtatctaa agaaaagccg caagtggcag aagtcttagc
      301 agttggtcct ggtagaacca atgatgatgg taaaaccttt cctatgccct ttaaaaaagg
      361 taataaagtt gtctatgcca aatattctgg aacagatatt aaaggggagg atgacgaaga
      421 ttaccttctt ttaagtgaaa aagatgtact ggctttactt gagtagtata gatatttaaa
      481 taaaaataat gataagaaca tattcatgat attattgatt tgcaaattta acgttataac
      541 attctattaa agaggagtta aaaaaaatat ggcaaaacag tttttatttg aggaaaacgc
      601 tagaagtgct cttaaaaaag gagctgataa gttagctgat agtgttaaaa taactttagg
      661 accaaaaggt aggaacgtag tattagaaaa aaaatacggt tcacctacta tcaccaatga
      721 tggagtgact attgccagag agattgaagt agaagaccct tttgaaaata tgggagcaca
      781 gtttgtaaaa gaagttgctt ctaaaacaaa tgatattgct ggagatggta ccactactgc
      841 aacagtttta gctcaaaaaa tacttcatga aggtatgaga aatgtcgctg ctggcgccaa
      901 tcctatgatg ctgagaagag gaatacagaa agccgtagat aaggctgtgg aagaaattaa
      961 aaaattgagt atagagatta aagataaaga atcaattgcc aatgtcgctt ctaattctgc
     1021 tgcagaacgt gaaattggta acattattgc agatgctatg gaaaaagttg ggaaagatgg
     1081 cgttatcacg gttgaagaat ctaaaacaat gggaactact ttagaagtag ttgaaggaat
     1141 gcagtttgat aaaggttata tttcgccata tatggttacc gatgccgaga ggatggaagc
     1201 ggtgttggaa gaacccttaa ttcttatcac tgatgctaaa ataagtaaca tgaaaggttt
     1261 gttgccggtt ttagaaaaag tggcacagat gggtaaacct ttatttataa ttgcagaaga
     1321 tgtcgaaggg gaagccctgg ctacattagt agtgaacaaa attagaggta ctctgaattg
     1381 tgtagcagta aaagctcctg gctttggaga tagaaggaaa gaaatgctaa ctgatattgc
     1441 tacggtaacc gggggacagg ttatttcaga agaactcggt ttgaaactgg agaatactga
     1501 tatttctatg ttaggtactg ctcatcaggt taaagctgac aaagaagaga ctataattgt
     1561 tggtggcaaa ggtgatgtta aggaaataaa gaagagagaa gcccaaatta gagctcagat
     1621 agaggaaaca acatcagatt atgacaaaga aaaattacag gaaagattag gtaagctggt
     1681 aggtggtgtg gccataatta atgttggcgc tgcaactgaa actgagctaa aggagaagaa
     1741 acatagagtt gaggatgccc tttcagcaac taaggcagcc gtagaagaag ggattgtagc
     1801 tggcggagga gctgtacttc ttaatattat tcctgcttta gaaaagctca aactggaagg
     1861 tgacgaacag attggtgtca atatcattct aaaagcatta gaagaaccta ccagacaaat
     1921 tgttaacaat gccggctatg aaggatcaat tatcgttgaa cagttgaaac acaaggagaa
     1981 aggtgttgga tttgatgccg aaaaaggaga atatgtagat atggtgaaaa gaggaatcat
     2041 tgatccaacc aaagttaccc gttctgcatt acagaatgct gccagtatag ccggaatggt
     2101 gctaactacc gagtgcttaa ttgcagataa gcctgaagat aagaaagaac cggttatgcc
     2161 tggtggaggc ggaatgcccg gtggaggaat gggaatgtat taactaagga aaaacataat
     2221 ttagttaatc aatttaatca atattcactg taataaaact aaagccccct ctttacaata
     2281 aagagggggc tttagtttta ttactacgaa aaagtcatta aagaaatctg aaaaaattaa
     2341 ttcaattctt caatagagga atatgggtat ccaaaagcat ctgccaccgg tttatatgtt
     2401 acccgtccat ctatagtatt taatcctttt gctatagctg gattacttat aattgctttt
     2461 ttatatcctt tattggcaat ttccaaggca taggacatgg tagcattagt taaagcaaaa
     2521 gtagaagtac ggggaaaggc gccaggaata tttggtacac aatagtgtaa gacatcatgt
     2581 aatacataag ttggttcact taaagaggta actctgctgg tttcaataca ccctccctga
     2641 tctatggcta catcgagtat gacggaacgt ttgggcatct tttttaccat ctcttctgta
     2701 acaactttgg gtgccggtgc cccaggaatt agaacagcgc ctattaaggc atcagcattt
     2761 tttagttctt cttcgatact gaatggattt gaagcaagag taactacatt tttaggcata
     2821 atgtcatcga gatatttaag gcgtttcaag ttaatatcaa aaataactac gctagctccc
     2881 ataccagaag ctactttagc cgcattggta cctacagttc caccacctac aaccaccacc
     2941 ttagccggtt ttacgccagg cacaccaccc aataagacac ctctgccacc tcggggaagt
     3001 gacagataat gagcaacaag attaccggct gttttaccgg ctacttcact cattggtgcc
     3061 aaaagtggaa ggtctccatc atcagtttca acagtctcat aggctataga gatacatttt
     3121 ctgtctaaca tggcttgagt aagctcgtga gaggaagcaa aatgaaaata agtaaatact
     3181 atctgacctt ctttgataaa agaatattct gaagggagag gctctttaac ctttataatc
     3241 atatcagctt cttcataaat ttcagcaggt tcttccaaaa ctatggcacc aacatctgaa
     3301 tattcctggt cagcaattcc acttcctata ccagctgctt tctgaacgaa aacctgatgg
     3361 ccgttctttg taaaagcggt aactcctgca ggagtgatag ctacacggtt ttctttatct
     3421 ttaatttctt taggaacacc aataatcatg gttggcaatt tcctccttct aactaaatta
     3481 at
//
LOCUS       DAOQWT010000108         3477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_28249_length_3477_cov_3.269433, whole genome shotgun sequence.
ACCESSION   DAOQWT010000108 DAOQWT010000000
VERSION     DAOQWT010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28249_length_3477_cov_3.269433"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PKJ95_02990"
     CDS             <1..310
                     /locus_tag="PKJ95_02990"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65244.1"
                     /translation="PDLKNIIITPICPHTLSARSLVVHHKDIIKLIIRSKGQEVMLTV
                     DGQEGFNLKENDVILVQESKYKTQLVTFPGKSFFSVLRKKLKWSGRVLPNIPEDQERP
                     "
     gene            314..2044
                     /gene="recN"
                     /locus_tag="PKJ95_02995"
     CDS             314..2044
                     /gene="recN"
                     /locus_tag="PKJ95_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNR65245.1"
                     /translation="MLNYLHIKNFALIDELSVEFEKGLNVITGETGAGKSMIIEAINV
                     ILGSPISQNWSKNDKECLEIEALFYLDAISRKQLQELQDINPTTFLDNQLIIRREIHQ
                     SRRSKCFINNQLVTLSVLQEIGNYLIDLHGQHSHQSLLNPLFHIDFVDSFENGLFSEK
                     KEKFYNHYKQWQTKKKILEEIKEKHSETLSKKDYLLYQLKEIEQAKLKNGEDQELEEI
                     VNIIRHKAKIKEVTEKAYDVLFDGNSIEEIPILDLLARLISSFDNISNLDHKINNIKE
                     QLSEVQLKIEDISSQILEYKEKIDLDVYQLQEIEERLNLINHLKHKYGSKIEDILKYN
                     DNLQKELSSIEDDEIKMEKLKKEIEVEEKILIELSLELSSQRKSIARKLEQDIILELA
                     ELNMKNCNFVIKIEQQEDKNGLPIADKMFKVTQDGIDRVEFFISTNVGEKIKPLTDIV
                     SGGEVSRIMLGLKSILSKADQIPIMIFDEIDSGVGARLGAIIARKLAKIAQNHQVIAV
                     THLPQIACQANQHLYISKYVSLNKTILSIKKLAGDEQLYEIARMLDGDKYGSISIEHA
                     EKMLHQKGDE"
     gene            2049..>3477
                     /locus_tag="PKJ95_03000"
     CDS             2049..>3477
                     /locus_tag="PKJ95_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNR65246.1"
                     /translation="MDKKDNIINKFLLREVHKQKEILSTLKAIINSTGDAISVVNEKG
                     IHTLVNDAYTRLIGLTEKDVLGQSSNIDIAEGESMHMKVLKTKEPVYGVKMKVGPNKK
                     EVIVNVAPVIVDGVLKGSVAIIHDLSEIRSLTEELNQVKKRVRHLEAKYTFDDIVGKS
                     RAMIIAKEQAIKAANTPATVLLHGESGTGKELFAHAIHNHSSRKNRQFIRVNCSALTD
                     TLLESELFGYESGAFTGASKKGRKGLFEEADRGTIFLDEIGMMSLNLQAKILRVLQEK
                     EIVRIGSNEPIIVDVRIISATNINLDEAVQKGLFREDLYYRLYVVPILIPPLRERKED
                     IPLLVQNIVRKCNQEFGRNIKDVSQEVIDLLSSYSWPGNIRELENVIERAVINMRVQD
                     NVLKQIHLPEYISLNRKKVEEQEGFNAEQKVETDGEIKTGGLKNMKGEIEKNAIIEAL
                     RAFKGDYQKVANFLGISIRTLYYKMS"
BASE COUNT         1323 a          472 c          698 g          984 t
ORIGIN      
        1 ccctgattta aaaaatataa ttattactcc catttgcccc catactcttt cggcaagatc
       61 tctggttgtt caccataagg atataattaa gttaatcatt cgttcgaaag gtcaggaagt
      121 aatgttaaca gttgatgggc aggagggttt taacttaaaa gaaaatgacg ttatcctggt
      181 tcaggaatct aagtataaga ctcaattggt aactttccct ggaaaaagtt tttttagtgt
      241 tttaaggaaa aaattaaaat ggagtggcag agttttacct aatataccag aagatcaaga
      301 aaggccttaa tgtatgctta actatctcca tataaaaaat tttgccttaa tcgatgaatt
      361 aagtgttgaa tttgaaaaag ggctgaatgt tattaccggg gaaaccggag ccgggaaatc
      421 aatgattata gaagctataa atgtaatttt aggttctccg atcagtcaaa attggtctaa
      481 aaatgataaa gaatgtttgg aaattgaagc cttattttat cttgatgcaa tatcccgaaa
      541 acaattacag gaattacagg atataaaccc aacaactttt ctggataatc aattaattat
      601 aagaagagaa atacaccagt ccagaaggag caagtgtttc ataaacaatc aactggttac
      661 cctttctgtt cttcaggaaa ttggcaatta tctaatagac ctacatggtc aacatagcca
      721 tcaatcatta ttgaaccctc tatttcatat tgattttgtt gatagctttg aaaacgggct
      781 tttctcggaa aaaaaagaga aattttataa tcattacaaa cagtggcaaa caaagaaaaa
      841 aatattggaa gaaataaaag aaaaacattc agaaacctta tcaaaaaagg attatctgct
      901 ctaccagctt aaagaaatag agcaggcgaa attaaaaaat ggagaagacc aggaattaga
      961 agaaatagta aatataattc gtcataaagc taaaattaaa gaagttacag aaaaggcata
     1021 tgatgtttta tttgatggta atagtataga agaaattccc attcttgatc ttttagccag
     1081 attgattagc agttttgaca atatttccaa tttggaccat aagataaata atatcaaaga
     1141 acaactatca gaagtacaat taaaaataga agatatctct tctcagatac tggagtataa
     1201 agaaaaaata gatctggatg tctatcaatt acaggaaatt gaagaacgcc tgaatttgat
     1261 taatcatctg aagcacaaat atgggtcaaa gatcgaggat atacttaagt acaatgataa
     1321 tttgcaaaaa gaattaagtt caattgaaga tgatgagata aagatggaaa agttaaaaaa
     1381 agagattgag gtagaggaaa agatattgat tgagttatct ttagaattaa gcagccagcg
     1441 aaaatcaatt gccagaaaat tagaacagga tattatactg gaattagcag agctaaatat
     1501 gaaaaactgt aattttgtaa tcaaaattga acaacaggaa gataagaatg gtttgcccat
     1561 tgcagataaa atgtttaagg ttactcaaga tggaattgac cgggttgaat tttttatttc
     1621 tactaatgtt ggagaaaaga taaagccttt aacagatatt gtatcgggag gagaggtttc
     1681 cagaattatg ttagggctaa aatctatcct aagcaaagca gaccagatac ctataatgat
     1741 ctttgatgaa attgacagtg gagtaggtgc ccgtctgggt gccattatag ctcggaaatt
     1801 agcaaagatt gctcaaaacc atcaggttat cgcggttact catctacctc agattgcctg
     1861 tcaggcaaac caacatctct atataagcaa gtatgtatct ctaaataaaa ctatcctgtc
     1921 tataaagaaa ttagcaggag atgagcagct ctatgaaatt gcccggatgc ttgatggaga
     1981 taagtatggc tctataagca ttgaacatgc tgaaaaaatg ttacaccaga agggtgatga
     2041 atagaagaat ggataaaaaa gataatatta ttaacaagtt tctgctcagg gaagtgcata
     2101 aacaaaaaga gattttgagt accctgaagg ctattatcaa ttctaccggt gatgctattt
     2161 cggtggtgaa tgaaaaaggt atacacactt tggtaaatga cgcctatacc agattgatag
     2221 gattaaccga aaaggatgta ttagggcaat cttccaatat tgatatagct gagggagaaa
     2281 gtatgcatat gaaagtttta aaaacaaaag agcctgttta tggtgtaaaa atgaaggtag
     2341 gccccaacaa gaaagaagtt atagtaaatg tggcaccggt aatcgttgat ggagttctaa
     2401 aaggaagcgt ggcaataatt catgaccttt ccgaaataag aagtttgaca gaagaattaa
     2461 atcaggttaa aaaaagagtt cgtcatttgg aggctaaata tacttttgat gatattgtag
     2521 gtaaaagcag agctatgatt attgccaaag agcaagctat taaagcagct aataccccgg
     2581 ctacagtatt attacatgga gaaagtggaa ctggtaaaga actttttgcc catgccattc
     2641 ataatcatag ttccagaaaa aatcgtcaat ttattagagt taattgttct gcattaacag
     2701 atacattact ggagagcgag ctttttggtt atgaaagtgg agcgtttact ggtgccagca
     2761 aaaaagggcg taaaggtctt tttgaagaag cagatcgtgg taccatattt ttggatgaaa
     2821 ttggtatgat gagtttgaat ttgcaggcta aaattttaag ggtgttacaa gaaaaggaaa
     2881 ttgttagaat cggcagtaat gaacccatca ttgtagatgt aagaattatc tcagcaacca
     2941 atattaatct ggacgaagca gtacagaaag gcctttttag agaagatcta tattaccggt
     3001 tatatgttgt tcccatactc atccctcctt taagggagag aaaagaagat attcccttac
     3061 tggtacaaaa tattgtcagg aaatgtaatc aggaattcgg cagaaacata aaagatgttt
     3121 ctcaggaagt aattgatttg ttgtctagct attcctggcc tggtaatatc agagaactgg
     3181 aaaatgtaat tgaaagagca gtaataaata tgagagtaca ggacaatgtt ttaaaacaga
     3241 ttcatcttcc tgaatatata agtttaaata gaaaaaaagt agaagaacag gaagggttta
     3301 atgctgagca gaaagttgag actgatggtg aaatcaaaac tggtggacta aaaaatatga
     3361 agggggaaat tgaaaagaat gctattatcg aagcactcag agcttttaaa ggtgattatc
     3421 aaaaagttgc aaatttttta ggaatcagta taagaacact atactataag atgagca
//
LOCUS       DAOQWT010000109         3473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_28284_length_3473_cov_22.572557, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000109 DAOQWT010000000
VERSION     DAOQWT010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28284_length_3473_cov_22.572557"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3473
                     /locus_tag="PKJ95_03005"
     CDS             <1..>3473
                     /locus_tag="PKJ95_03005"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR65247.1"
                     /translation="SFTTGEEFYQPIISVTPTSIYDELTTEETSQTEITIANNGTAPL
                     IWNSNIVLTRAEQKTGTNAYLTKENNTERELNAKIENIDANSIRDKDMNREGTSPMPE
                     INLSPSSGTIEPNSSVICILNFIPGDSLGTYNYELQLSSNDPVSPLITVTLEYVVTSP
                     IAYIPDNNFRRAINAALGQPSDYQPTIADLNALTGTLSAAVRNITSIEGAQYLINLQD
                     LDLYYNQISGLSPLAGLTNLQLLYLNNNQISDLSPLAGLSNLQKLYLGVNQISDLSPL
                     AGLTNLQGLNLTSNQISDLSPLAGLTNLSYLLLENNQISDLSPLAGLTNLQELWLSNN
                     QISNLSPLEELTNLQDLALVENQISDLSPLTGLSNLQYLTLYNNQISDLSPLAGLSNL
                     RTLHLGDNQISDLSPLTELTNLQQLGLQINPISYESMLLSQNWYLPWSTDEYNSLSPC
                     YPVPNRDAINVSIGNDLFWQGNYGNSQDVFYEVWLGISPDSLFSQGLETQMVNATEHL
                     YSFTPMLEPLTQYYWRIRAIAPTDTIWSGMWSFTTGEEFYQPIISVTPTNIYAELTTA
                     ETSQTEITIVNYGTATLIWNSNIVLTRAEQKTGTNAYLTKENNTERELNAKIENIDAN
                     SIRDKDMNREGTSPMPEINLSPSSGTIEPNSSVTCILNFIPGDSLGSYNYELQLSSND
                     PSSPLITVTLEYVVTTIAYIPDNNFRMAINEALGQPSEYQPTIADLNGLTGALSANER
                     NIISIVGAQYLTHLQYLYLNQNQISDLSPLAGLTNLQYLYLGDNQISDLSPLAGLINL
                     RELNLYNNQISDLSPLVGLTNLRRLGLYDNQISDLSPLACLTNLLVLWLKNNQISDLS
                     PLAGLTNLQYLYLWSNQINDLSPLEGLTNLQFIHLSDNQISDLSPLAGLTNLHELELG
                     SNQISDLSPLAVLTNLNFLDLSGNQIIDLSPLAGLTNLQELWLSYNQISDLSPLEELT
                     NLQELYLHYNQISDLSHLAGLTNLQELWLSYNQISDLSPLEGLTNLQYLYLQSNQISD
                     LSPLEGLTNLQYLLLWSNQISDLSPLAELSNLQKLWLNSNQISDLSPLAGLTNLNYLN
                     LNYNQISDLSPLDRLTNLQQLYLCGNKISDLNPLLGMTHLVYLYLDYNPISYESMLLS
                     QSWSLPWSTNE"
BASE COUNT         1032 a          708 c          638 g         1095 t
ORIGIN      
        1 agtttcacta ccggtgagga attctatcag ccaattattt ctgttactcc taccagtatt
       61 tatgatgaat taactactga ggaaaccagt caaacagaaa taactattgc caataatggc
      121 actgctcccc ttatctggaa tagcaatatt gttttaaccc gggcagaaca gaaaacagga
      181 actaatgctt atcttactaa ggaaaataac acagaaagag aattgaacgc taaaattgaa
      241 aacatagatg ccaatagcat aagagataaa gatatgaata gagagggaac atccccaatg
      301 ccggaaatca acctttctcc ttcctccgga actatagaac ctaattcttc ggtaatctgt
      361 atcctgaatt ttattcctgg agattccctt ggcacctata attatgaact tcagctctca
      421 tccaatgatc ctgtttcacc tctgatcacg gttactttag aatatgtagt taccagtcct
      481 attgcctata ttcctgataa caattttaga agggcaataa atgcggcatt aggtcaacct
      541 tctgattatc aaccaactat tgctgatttg aatgcattaa ctggaacttt atctgctgcg
      601 gttcgcaata taacaagtat agaaggtgct caatatctga ttaatctgca agatctggat
      661 ctttattata accagataag tggcctcagt cctttagctg gtctgactaa tctgcaattg
      721 ctgtatctta ataataacca gataagtgac ctcagtcctt tagcgggtct gtctaatctg
      781 caaaagcttt atcttggtgt taaccagata agtgacctca gtcctttagc gggtctgact
      841 aatctgcaag ggttgaatct tacttctaac cagataagtg acctcagtcc tttagcggga
      901 ctgactaatt tgtcttatct acttcttgaa aataaccaga taagtgacct cagtccttta
      961 gcgggtctga ctaatctgca agagctgtgg ctttctaata accagataag taacctaagt
     1021 cctttagagg agctgactaa tctgcaagat ctggcgcttg ttgaaaacca gataagtgac
     1081 ctcagtcctt taacgggtct gtctaatctg caatatctta cgctttataa taaccaaata
     1141 agtgacctca gtccattagc ggggctgagt aatcttcgaa cgttgcatct tggtgataac
     1201 cagataagtg acctcagtcc tttaacggag cttactaatc tgcaacagct gggtcttcag
     1261 attaatccta tttcttatga gtcaatgctg ctgtctcaaa attggtattt accttggtca
     1321 accgatgaat ataattccct ttcgccttgt tatcctgttc caaatagaga tgcaataaat
     1381 gtatctatag gcaatgattt attttggcag ggtaattatg gtaatagcca agatgttttc
     1441 tatgaggtct ggttgggcat tagtccagat tcccttttct ctcagggctt ggaaactcaa
     1501 atggtaaatg ctacggaaca tctttattcc ttcactccaa tgctggaacc cttaacacaa
     1561 tactattgga gaataagagc tattgcccca actgatacaa tctggagtgg aatgtggagt
     1621 ttcactaccg gtgaggaatt ctatcagcca attatttctg ttactcctac caatatttat
     1681 gctgaattaa ctactgcaga aaccagtcaa acagaaataa ctattgtcaa ttatggcact
     1741 gctaccctta tctggaatag caatattgtt ttaacccggg cagaacagaa aacaggaact
     1801 aatgcttatc ttactaagga aaataacaca gaaagagaat tgaacgctaa aattgaaaac
     1861 atagatgcca atagcataag agataaagat atgaatagag agggaacatc cccaatgccg
     1921 gaaatcaacc tttctccttc ctcaggaact atagaaccta attcttcggt aacctgtatc
     1981 ctgaatttta ttcctgggga ttcccttggc agctataact atgaacttca gctctcatcc
     2041 aatgatcctt cttcacctct tatcacagtt actttagaat atgtagttac tactattgcc
     2101 tatattcctg ataacaattt tagaatggca ataaatgagg cattaggtca accctctgaa
     2161 tatcaaccta ctattgctga tctgaatgga ttaactggag ctttatctgc taatgaaagg
     2221 aatataatta gtatagtagg tgctcaatat ctaactcatc tgcaatatct atatcttaat
     2281 cagaatcaga taagtgatct cagtccttta gcggggctaa ctaatttaca atatctgtat
     2341 cttggtgata accagataag tgacctcagt cctttagcgg gtctgattaa tttgcgagag
     2401 cttaatcttt ataataacca gataagtgac cttagccctt tagtggggct gactaatctg
     2461 agaaggctgg gtctttatga taaccagata agtgacctca gtcctttagc gtgtcttact
     2521 aatctgttag tactttggct taagaataac cagataagtg accttagtcc tttagcgggg
     2581 ctgactaatc tgcaatattt gtatctttgg tctaaccaga taaatgacct cagtccttta
     2641 gagggtttga ctaatctaca gtttattcat ctttctgata accagataag tgacctcagt
     2701 cctttagcgg ggctgactaa tctgcacgag ctggagcttg gttctaacca gataagtgac
     2761 ctcagtcctt tagcggttct gactaatctt aattttctgg atcttagtgg taaccagata
     2821 attgacctca gtcctttagc tgggctgact aatctgcaag agctgtggct ttcttataac
     2881 cagataagtg accttagtcc tttagaggag ctgactaatc tgcaagagct gtatcttcat
     2941 tataaccaga taagtgacct cagtcattta gctgggctga ctaatcttca agagctgtgg
     3001 ctttcttata accagataag tgacctcagt cctttagagg gactgactaa tctgcaatat
     3061 ctgtatcttc aatctaacca gataagtgac ctcagtcctt tagaggggct tactaatctg
     3121 caatatttgt tgctttggtc taaccagata agtgacctca gtcctttagc ggagctatct
     3181 aatctgcaaa agctgtggct taattctaac cagataagtg acctcagtcc tttagctggt
     3241 ctgactaatc tgaattatct taatcttaat tataaccaga taagtgacct cagtcctttg
     3301 gataggctaa ctaatcttca acagttgtat ctttgtggta acaagataag tgacctcaat
     3361 cctctattag gtatgactca tctggtgtat ctttatcttg attataatcc tatttcttat
     3421 gagtcaatgc tgctgtctca gagttggtct ttaccttggt caaccaatga ata
//
LOCUS       DAOQWT010000110         3468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_28350_length_3468_cov_3.269851, whole genome shotgun sequence.
ACCESSION   DAOQWT010000110 DAOQWT010000000
VERSION     DAOQWT010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28350_length_3468_cov_3.269851"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PKJ95_03010"
     CDS             <1..999
                     /locus_tag="PKJ95_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HNR65248.1"
                     /translation="NEWREKYAEELPCIMVQIESNVVIPVESASNKEEFALASFRKKM
                     QRLLADYLQPIDMSEPVQSSLNLNIASFPIDDLRESVNQLSIDNSVSPSPFFRGGEEE
                     AHVLLDRFIAEKIYRFGTLRNNPALDFLSHMSPYLHFGQISPLYITLKLKAKAPSEAY
                     RLYIEELIVKRELAINHVFYNDHYDQFKGLPRWARSTLEKHQNDTRKVIYSIAELEHA
                     LTGDPYWNAAQQEMMISGKMHGYMRIYWAKKILEWSPTPQEAFQRCLYLNNKYALDGR
                     DPNSYSGIAQCFGLHDRAWKERPIFGKVRYMDENHLTRKFDMEKYVEKVKELKKTN"
     gene            1091..1780
                     /locus_tag="PKJ95_03015"
     CDS             1091..1780
                     /locus_tag="PKJ95_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HNR65249.1"
                     /translation="MNIKSTIKEVMQAIIPLALVIILFQFTAIRLPIKDFLYFLLGVL
                     LAFVGFVLFLIGARVSVLPMGETFGASLAQDEKLWLLIGLGISLGFAVTISEPGLKVL
                     ANQVSDVSNGEIPKNLLMITVSLGLGIFLALSLLRVIFKIPLQIMILGSYITAFLLAM
                     FSSPEFFAVSFDAGGVATGPMTVPFIIALGVGLSSVRGTRSASADSFGFVGLAYAGPI
                     LAVLLLGMIYR"
     gene            1777..2487
                     /locus_tag="PKJ95_03020"
                     /pseudo
     CDS             1777..2487
                     /locus_tag="PKJ95_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505378.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
     gene            2743..3072
                     /locus_tag="PKJ95_03025"
     CDS             2743..3072
                     /locus_tag="PKJ95_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNR65250.1"
                     /translation="MAEDRIKFDLIITVVNRGYATDVIQISRKAGARGGTIICGRGAG
                     IHEDKKIFGIPIEPEKEIILTIIEHDKTDVVLEAIKKELKLDEPGKGIAFVLDVEKVV
                     GITNQTC"
     gene            3163..>3468
                     /locus_tag="PKJ95_03030"
     CDS             3163..>3468
                     /locus_tag="PKJ95_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR65251.1"
                     /translation="MPRQRRAYSSTQIYHIMVRGNAGRDIFLDDDDKKQFLRILSRKK
                     RQTEFKLYAFCLMKNHFHLSLKEGNENISNIMKRINTTYAVYFNKKYNDIKNGTKQIR
                     "
BASE COUNT         1103 a          527 c          767 g         1071 t
ORIGIN      
        1 aatgaatggc gggaaaaata tgcagaggag ttgccctgta ttatggttca gatagagagc
       61 aatgtggtaa ttccggtaga gagtgcctcc aacaaagagg agtttgccct ggcttccttc
      121 cgaaagaaga tgcaacgctt gttagcagat tatcttcagc cgatagatat gagtgaacca
      181 gtacaatctt ctctaaatct aaatatagcc tcatttccca tagatgattt aagggaaagt
      241 gtgaatcaac tatccataga taattcagtg tctccatccc cattttttcg gggaggggaa
      301 gaagaagccc atgtcctgtt ggatagattc attgcagaaa agatataccg ttttggcact
      361 ttacggaaca atccagcgct ggatttttta tcacatatga gtccctatct ccattttggt
      421 cagatatcac ccctttatat tacccttaag ttgaaagcaa aggcgccatc tgaagcctat
      481 aggttatata tagaagaatt aatagtaaaa agagaactgg ctatcaatca tgtcttttat
      541 aatgaccatt atgaccagtt taaaggttta cccagatggg ccaggagtac tctggaaaaa
      601 catcaaaatg ataccagaaa agtaatttat tccatagccg aattggaaca tgccctgacc
      661 ggtgaccctt actggaatgc tgcccagcag gagatgatga taagtggaaa gatgcatgga
      721 tatatgagga tttactgggc taagaagata ttggaatgga gccccacacc tcaggaagct
      781 tttcagcgct gcctctatct aaataataaa tatgctttag acggaagaga tcccaattct
      841 tattccggaa ttgcgcagtg ttttggattg catgaccgtg cctggaaaga gaggcctatc
      901 tttggcaaag ttcgctatat ggatgagaat cacctgacca gaaaatttga tatggaaaaa
      961 tatgtagaaa aggttaaaga attaaaaaag acgaactgag aagaatcttt tttaaattcc
     1021 ttatattgga agattgagtc atatctaaat atagatttaa aagtggctga agaggacaga
     1081 agtagaagct atgaacatca aaagcaccat aaaagaagta atgcaagcca tcatacctct
     1141 tgctttagtg ataatacttt ttcaatttac cgctatcaga cttcccataa aagattttct
     1201 ctattttttg ttgggggttt tacttgcttt tgtaggattt gttctatttt tgattggagc
     1261 aagagtcagt gttttaccaa tgggtgaaac atttggagca tcgttggctc aggatgaaaa
     1321 gttatggttg ttaattggac tgggtatttc tttagggttt gccgttacca tatctgaacc
     1381 aggattaaag gtgctggcca accaggtgtc agatgtttcc aatggtgaaa taccgaaaaa
     1441 tttattaatg ataacagttt ctctgggatt aggaatattc ctggctcttt cgttactcag
     1501 agttattttt aaaattccat tacaaatcat gatattaggg agttatatta cggcattttt
     1561 gctggccatg ttttcgtcac cagaattttt tgctgtttct tttgatgctg gtggagtggc
     1621 aacaggtcct atgaccgtgc cctttatcat tgcgctggga gtaggattgt cctcagttcg
     1681 gggtaccaga agtgcttcgg cggatagttt tggttttgta gggttagcct atgcgggacc
     1741 tattttagcg gtattacttt tagggatgat atatagatga attcaatatt cagtcaactt
     1801 atgggggtag caaaagaggt agtccaatcc tcactacccc tcctgataat atttttaata
     1861 tttcagtcgg taagactaaa attgcccaaa tcacaattca taatcattat gaggggtttc
     1921 gtcttaacct ttgtgggttt gggcctcttt ttactgggaa taaatattgg atttgtaaaa
     1981 gcgggggaat tgattggaaa agggttgggg ggattggagc ataactggat tttgataccg
     2041 gtaggcttct tactaggact gttagtaacc ctggctgaac ccgcggtacg tattttaaat
     2101 attgaagtag aaaaagtgtc agccgggtat atcaatagaa caattttact ctattttcta
     2161 tcttttggtg tggcaattgc gatagcctta tctatgataa ggatgttaaa aggaatatct
     2221 ttgtggtatt ttttattgcc gggttacata gcgatcttaa ttttaatgtg gtttgtaaag
     2281 cccatatttg tagcggtggc ttttgatgcc ggaggcgcgg ttaccagtcc catggttgtt
     2341 accttcctga tggctctaac agtgggaagc tccaaggcaa ttgttcatag caatccgctc
     2401 ttagatgctt ttgggatgat agcaatggtg gctgtggttc cggtcttttc catacttttt
     2461 ctgggtgttt tatatcagag gaaggaagtt gctaataata atggagttca taatagcagt
     2521 agtagtaata aaaatagtaa taatggcaat gataaaaaag ataatagtgg taatggtata
     2581 gttgaggaga aaaagaaggt tgataggaag ggcagttgcc ttactgagta agaaaaggtt
     2641 cggtctgtaa aaatatttat atgattactc tattacacaa tacgaaacga ctatggaaat
     2701 taaggggaat aaagtaattt taaaacaggg ggaattgaaa taatggctga agatagaatt
     2761 aaattcgatt tgattattac agttgtaaat aggggctatg ccacagatgt tattcaaatc
     2821 tctagaaaag caggagcaag aggtggtacc attatctgtg ggcgtggtgc agggatacat
     2881 gaagataaaa aaatatttgg catacctatt gaaccggaaa aggagattat ccttactatt
     2941 attgaacatg acaagacaga tgtagtattg gaagcaataa agaaagaatt aaaactggat
     3001 gaaccgggta aaggaatagc ctttgttttg gatgtagaaa aggtggttgg cataactaac
     3061 caaacatgtt aggggacggt cctttgacat ctttcttttt agatgttaaa attattccat
     3121 tcgcaaattc ataagtagac tcataaaagg gagtaattat ttatgcccag acaaagaaga
     3181 gcatacagct caacacaaat ttaccatatt atggtacggg ggaatgccgg tagagatatt
     3241 tttttagacg atgatgataa aaagcagttt ctgagaatac tttctcgtaa aaaaagacaa
     3301 actgaattca aattatatgc tttttgtctg atgaaaaatc attttcatct atctttaaaa
     3361 gaaggaaatg agaatatctc taatattatg aaaagaataa acactactta tgccgtatat
     3421 tttaataaaa aatataatga cattaaaaat ggaacaaaac aaattaga
//
LOCUS       DAOQWT010000111         3466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_28383_length_3466_cov_2.433011, whole genome shotgun sequence.
ACCESSION   DAOQWT010000111 DAOQWT010000000
VERSION     DAOQWT010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28383_length_3466_cov_2.433011"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1670
                     /gene="selA"
                     /locus_tag="PKJ95_03035"
     CDS             246..1670
                     /gene="selA"
                     /locus_tag="PKJ95_03035"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNR65252.1"
                     /translation="MIENNNRNNRENLHSLLSRIPSVEEVLQLEELKLPASQYSRKMI
                     TPLVRLVLEEERERIKSGSVPSPLRDITDRCASLIAKEFQSFIRPVINGTGVILHTNL
                     GRAVLGQTIIDDSLPSLAGYCNLEYDLFQGVRGKRGDFVEKMLALFSQTESSLILNNN
                     AAAVFLILKVLAKGKEVIISRGELVQIGGGFRIPEILKESGAILREVGTTNQTNLDDY
                     EKAINENTALLLKVHQSNFSLEGFSESVEVGELKALGEKYNLPVVVDLGSGTFLPTEQ
                     FDLLHEPMVQEMIRCGADLVCFSADKLLGGPQGGIVCGRKEYISQIRKDPLYRTFRVG
                     KITLSLLQSTLLFYLQGRTLQELPVWRMISLPAQEIKKRSHSLARTLRKRDIPTQVSQ
                     GISLVGGGSLPGKTLSTYLVSIETDGNVDQLDRRLRMSNPAVLGRVKDNRLFFDLRTI
                     EPKFDKKLIELISAAFTKNMNNKG"
     gene            1687..>3466
                     /gene="selB"
                     /locus_tag="PKJ95_03040"
     CDS             1687..>3466
                     /gene="selB"
                     /locus_tag="PKJ95_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HNR65253.1"
                     /translation="MVIFGTAGHIDHGKTSLIYALTSINADRLPEEKLRGMTIDLGFA
                     WMETPLGEKIGIIDVPGHEHFIRNMITGITSVDAFIMVVDAKEGWKEQTEEHFQIIKL
                     LEINYGLIVITKTDLVLPERLKEVKEEIEERVNSKDRPNIPILYFSYSNRDSVSQLRA
                     EVEKLSRTIPPKRDIDKPRLFIDRVFEIKGSGTVITGTLLNGNLYQNQSIFHFPSLKK
                     VRLRQIESYNTSLEKAIVGNRVALNLIGLKKEDIARGDLIYANQKMISGNTYDVSAQL
                     IPQKTLFRLKNGNEVEIISHTKILRGMIIFEKKEVNPGEKFYAQIRFKEPLSLMLGDY
                     FIIRLPGINETIGGGRILAEQASRHSFRNSRWGKWLAKRDRLDIDRLIVSELERYQKI
                     KKEELLIDSPYAKEEINQHLEQLYQKALLFLKGDWVVDLKFWNMMCSQIVTFLEQKHS
                     DEPLKDGFPTIILKNKFPEIPEDLLFVLLDYLSEKGKVKLKKGIISSASHSINLSAQQ
                     NKVIEEILHYIERESSNLPTEKELKDVFPRNVALIEYLIEQGEIIILEGQIIITPVIY
                     KDMKEKIVNFLSKNHSITIGQVRDLLH"
BASE COUNT         1134 a          550 c          765 g         1017 t
ORIGIN      
        1 gggcggtggt tttaaaatta ccgcggtggg ttcgattccc acccctctcg ccactttatt
       61 gtcaatgccc attgatggtg tcaaaatgcc agcagaatgg gcattttgtg ttatatttac
      121 tttgtgatag aatatattat cgtttttaac cctttattat aatttaaaat gtattgctaa
      181 gaattaggta aatattatcc tggctttata gcttaatatt ctaaaatgag gagaaataat
      241 ttagtttgat cgaaaataat aatagaaata acagagaaaa tcttcattca ctattatccc
      301 gaattcccag tgtagaggaa gttttacagc tggaagagtt aaaattacct gccagccagt
      361 attcacgaaa aatgattacg cccctggtga ggctggttct ggaagaggaa agggaaagga
      421 taaaaagtgg ttctgttcct tctcctttaa gggatattac tgacagatgc gcctccttga
      481 tcgctaaaga attccaatct tttatacgac ccgtgataaa cggaacaggg gtgattttgc
      541 atactaatct ggggagagca gttctgggac agaccattat tgatgattct ttacctagct
      601 tggcaggtta ttgtaatctg gaatatgatc tttttcaggg agtgaggggt aaaagagggg
      661 attttgtaga aaagatgttg gctttattca gtcaaacaga aagttccctg attctcaata
      721 acaatgccgc ggcagttttt ctgattttaa aagtgctggc aaaaggaaaa gaagtaatta
      781 tctcccgggg agaattagta cagattggcg gtggttttcg tattcctgaa atattaaagg
      841 aaagtggtgc aatcctgcgc gaagtgggaa ctaccaatca gaccaatctc gatgattatg
      901 aaaaagcaat aaatgaaaat actgcattgc tgctgaaggt tcaccagagt aatttttctt
      961 tagaaggttt ttctgaatca gtggaagttg gggagcttaa agctttagga gaaaaataca
     1021 atctgccggt ggtagtggac ctgggtagtg gtacgttcct gcctaccgag cagtttgacc
     1081 tgctccacga accaatggta caggaaatga taagatgtgg tgctgacctg gtctgcttta
     1141 gtgctgataa gttgttaggg gggccccagg ggggaattgt ttgtggcagg aaggaatata
     1201 taagtcaaat tagaaaagac cccttatatc gtacttttcg ggttgggaaa attacgctct
     1261 ctcttcttca gtctactctg ttattctatt tacagggtag gactttgcag gaactgccag
     1321 tatggagaat gatttctttg ccggctcagg aaattaaaaa aagaagccat tctctcgccc
     1381 gcactttaag gaagagagac attcccactc aggtgagcca gggaatatct ctggtgggtg
     1441 gcggatcgct gccagggaaa acattgtcta cctacctggt cagtattgaa accgatggga
     1501 atgttgacca attggatagg agactgagaa tgtctaatcc agcagttttg ggaagagtta
     1561 aggataatcg tttgtttttt gatctcagaa caattgagcc taaattcgat aagaaattaa
     1621 ttgaactaat ctccgctgct tttactaaaa atatgaacaa taaaggatga ggctaaataa
     1681 ttttctatgg ttatttttgg aactgccggt catatcgatc acggtaaaac cagtttaata
     1741 tacgccttaa ccagtattaa cgcagaccga ttacctgaag aaaaattaag agggatgact
     1801 attgacctgg gctttgcctg gatggagaca cctcttgggg aaaaaattgg tattattgat
     1861 gttcccggtc atgagcattt tataagaaat atgattaccg gtataacctc tgttgacgcc
     1921 tttattatgg ttgttgatgc caaggaaggc tggaaagaac agacagagga gcattttcaa
     1981 ataattaaat tattagaaat taactatggg ttgatagtga ttactaaaac tgacctggtt
     2041 ttacctgaac ggctaaagga agtaaaagaa gagattgagg aaagagttaa cagtaaagac
     2101 cgaccaaaca ttccaattct ctattttagt tacagcaata gggatagtgt ttctcaatta
     2161 agggctgagg tggaaaaact gagcagaact attcccccga agagagacat tgataaacca
     2221 cgactgttta ttgaccgtgt ttttgaaata aaaggcagtg gaactgtgat taccgggacc
     2281 ctgctcaatg gtaatttata tcagaatcaa tcaatatttc attttccttc actgaagaaa
     2341 gttcgtttaa gacagataga aagttataat acttctcttg aaaaggctat tgtgggcaat
     2401 agagtggctc ttaaccttat cggattgaaa aaagaagata tagcaagagg agatttaatc
     2461 tatgccaatc aaaaaatgat atcaggaaat acctatgatg tttccgcaca acttattcct
     2521 caaaaaaccc tttttcggtt gaaaaatggt aatgaggtgg aaatcatttc tcatactaaa
     2581 attttgaggg gtatgatcat atttgagaag aaggaagtga atccgggaga aaaattttat
     2641 gcccagattc gcttcaagga gccacttagc ttgatgctcg gcgattattt tattattcgt
     2701 ttacctggta taaatgaaac cattggtggt ggaagaattc ttgctgaaca agccagcaga
     2761 catagtttcc gaaactctcg ctggggaaaa tggttagcaa agagggacag attggatata
     2821 gaccggttga ttgtaagtga attggagcga taccagaaga ttaaaaagga agaattgcta
     2881 attgatagcc cttatgctaa ggaggaaatc aatcagcatt tagagcaatt gtaccaaaaa
     2941 gctctcctct ttttaaaagg ggactgggta gttgacttaa agttctggaa tatgatgtgc
     3001 agtcaaatag tcacattcct ggaacaaaaa catagcgatg aaccattgaa agatgggttt
     3061 cccactataa ttctgaagaa caaatttccg gaaattccgg aagaccttct ctttgtttta
     3121 ctcgattatc tatctgaaaa aggtaaggta aaattaaaaa aaggaattat ttcttctgcc
     3181 agccattcta taaatctatc agctcagcaa aacaaagtga ttgaggaaat tttacactat
     3241 atcgaaagag agagcagcaa tttacctacc gaaaaagaat taaaagatgt ttttccaaga
     3301 aatgtagcac ttattgaata tttaattgaa cagggagaaa ttattatact ggaagggcaa
     3361 ataataataa ctccggttat ttataaagat atgaaagaaa aaatagtgaa ttttttaagt
     3421 aagaatcatt caattaccat tggacaggtt cgtgatttat tacata
//
LOCUS       DAOQWT010000112         3437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_28722_length_3437_cov_2.994678, whole genome shotgun sequence.
ACCESSION   DAOQWT010000112 DAOQWT010000000
VERSION     DAOQWT010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28722_length_3437_cov_2.994678"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PKJ95_03045"
     CDS             complement(<1..980)
                     /locus_tag="PKJ95_03045"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNR65254.1"
                     /translation="MRKKNIFIWFLMIVVIGSLIYFWREHLPIPFLNSNPGNSDNAYM
                     ENIDPRAVVEVKRGSIKKTVSASGYIKPVNEMYLSFASTGTTGGTVEKILVKRGEIVV
                     KGQELVKLEDKQEHLNYLKALNKYELAKITGSPSQIEEKKLTMEVALDRYESKTLPAP
                     FSGKIVDIFVEEGDFIEGTRDVIYIADDSSYEVTSSIGEIDILQVAVGQKVEIELDIL
                     KGQKFPGTVAEVAEYARIEGGVVTVPLTLRIDQVSPYFKPGFSATAEIIVDMIEDVLI
                     VPVTALSTSSRGSMVLKVDGDKAIPTPVNTGITDGFYQEIKNGLQEGDKI"
     gene            complement(991..2445)
                     /locus_tag="PKJ95_03050"
     CDS             complement(991..2445)
                     /locus_tag="PKJ95_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR65255.1"
                     /translation="MKSLNLREKMYKIIFILLTGLFLILNFQILKAKEISVEEALQWG
                     VLHNLDLQSIRYSIEDIKRNLEIVEAGKSFQIDLSITPIWRFGEKDNPYLVEIGENRV
                     APDTKLNLSAKKMLTPNLSMSGEVSWQSENSTEHFIEAIAEEVKANIKLEQKLYPRSW
                     TEQERQTYSLENNLQMKLEELKWKEIEKQIEFIQKYLNIIRLQEQLEIASERLRLAEE
                     ALERVRAQIKLGEGGFQQEAEAIISLGETQNKHWSTEQDLNMAKRQWYLLLNLPEEAT
                     VDFATEALFIDSLFQQMENLPIFSQSRDIFINQALQENYQFKNSVIEKDELLKELQWT
                     KDAGKPVVNLSGGHTYPDSDWFVMVDFRINLADGGIQELKVKQKEENVKRKEVNLVYL
                     MEMLRLEAEQLLDKDEYNQLILQTLSKSLEKEQEKEKIIEQQYHQGAISLAQRDNNLI
                     LLKEKELGLKQASDQWLVDRLKLAHLIGLLPKGV"
     gene            complement(2442..>3437)
                     /locus_tag="PKJ95_03055"
     CDS             complement(2442..>3437)
                     /locus_tag="PKJ95_03055"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR65256.1"
                     /translation="GQEKVVLDISRMISLTISNNLNFKKAIYQLNNAELDIRQLEAEN
                     LLTQSGILNKQKEINILQQQNTFQNQKSQLIIQAADNYFRLVLAELDIRRKEKNIDLE
                     KAVLAEVEAQVAAGYRIELDLLQQGNAYYDALFSYEKARLDYQQLLIEIKNALGLSGE
                     AEISVEAVSLPHFPDIDCNVSCEKARENSFTLKSKGIEVELALFKRENAKIEQASQLS
                     IAKLINNLEIVKLEQIILEQDLDYQILTQWQNHQQSKNSVLLAEQSLTQMKENESIIK
                     RQVQAGLRREDELLSASIGVLDAEYRFISSVRQCYQSYLELQKIMGILDEGAMVK"
BASE COUNT          938 a          780 c          516 g         1203 t
ORIGIN      
        1 attatcttat ccccttcctg taaaccattt tttatctcct gataaaatcc atcagtaata
       61 cccgtattca caggagtagg aatcgcctta tctccatcaa ctttcaatac catactgccc
      121 cggctacttg tagatagggc agtaaccgga acgattagga catcttcaat catatcgaca
      181 atgatttctg cagttgccga aaaacctggc ttaaaatatg gagatacctg gtctatccga
      241 agagttaacg gtacggtaac aacaccccct tctatacggg catattctgc aacttcggct
      301 actgtaccgg gaaatttttg acctttcaga atatcaagct caatttctac cttttgtccc
      361 actgcaactt gtaaaatatc tatttcacca atggaactgg tcacttcata tgatgaatca
      421 tctgcaatat aaatcacatc acgcgttcct tcaataaagt ctccttcttc tacaaaaata
      481 tctactattt ttccagaaaa aggtgctggc aatgttttcg attcataacg atccagggct
      541 acttccatag ttaacttttt ttcttctatc tgactgggtg aaccggtaat ttttgccagt
      601 tcatacttat tcaaggcctt taagtaattg aggtgttcct gtttatcttc taatttcact
      661 aattcctgtc cctttacgac tatttcccct ctttttacca gtattttttc cactgtacca
      721 ccggtagtac ctgtactggc gaaactgaga tacatttcat taaccggttt gatatacccg
      781 ctggctgata ccgtcttttt tatgctgccc ctttttacct caactaccgc tctgggatca
      841 atattttcca tataagcatt atcactattt cctggattgg agttaagaaa agggatgggc
      901 aaatgttctc tccagaaata tattaaacta cctattacca caatcattaa aaaccagata
      961 aaaatatttt tctttctcat tctaaatttc ttaaacccct ttcggtaaaa gaccaataag
     1021 atgagctagt ttaagacgat caactaacca ctggtcagaa gcctgcttta aacctaattc
     1081 tttctctttt aataatatca ggttattatc ccgttgagcc agactaatag ccccttgatg
     1141 gtattgctgt tcaattatct tctctttttc ctgttctttt tctaaggact ttgacagagt
     1201 ttgtaatata agctggttat attcatcttt atctaatagt tgttcggcct ctaatctcaa
     1261 catttccatt aaataaacca gatttacttc tttgcgtttt acattttcct ctttctgttt
     1321 tactttaagt tcttgaatac caccatcagc caggtttatt ctgaaatcaa ccataacaaa
     1381 ccagtcacta tccggataag tatgtccacc tgaaagattc actacaggtt taccggcatc
     1441 ttttgtccat tgcagctcct ttaacaattc atctttttct attacactat ttttgaactg
     1501 gtaattctcc tgaagagctt gatttataaa tatatcacga gattggctga agataggtaa
     1561 attctccatt tgctgaaaaa ggctatctat aaaaagagct tctgttgcaa agtctacggt
     1621 tgcctcttca ggcaagttaa gcaggaggta ccactgcctc ttagccatat tcaggtcctg
     1681 ttctgtactc caatgtttat tctgtgtctc accaagagaa atgatggctt ccgcttcctg
     1741 ctgaaaaccc ccttcaccca gttttatctg agctcttacc ctctccagtg cttcttctgc
     1801 cagtctcaac cgttcagagg ctatttcaag ttgctcctgt aaacggatta tatttaagta
     1861 tttctggata aattcgatct gtttttctat ttctttccac tttaattctt ctaacttcat
     1921 ctgaagatta ttttccagag aataagtctg tctttcctgt tcagtccagc tacgtggata
     1981 gagtttctgc tctaacttga tatttgcttt tacttcttct gctattgctt ctataaaatg
     2041 ttctgtcgaa ttctctgact gccaggaaac ttcgccagac atacttagat taggtgttaa
     2101 catttttttt gccgatagat ttagcttggt atcaggggca acccgatttt ctccaatttc
     2161 taccagatag ggattatcct tttctccaaa acgccagata ggtgtaatac tcaaatcaat
     2221 ctgaaatgac tttcccgcct ccactatttc caggttgcgt ttgatatctt ctatactata
     2281 gcgaatactt tgtaaatcaa ggttatggag aacgccccat tgcagggcct cttccacgct
     2341 aatctctttg gctttgagta tttgaaaatt tagtatcaaa aataagcctg ttaaaagaat
     2401 gaagattatt ttatacattt tttctctcaa atttaaagat ttcattttac catcgctcct
     2461 tcatccaaaa tacccattat tttctgcaac tccagataag attgataaca ctgccgcact
     2521 gaggaaataa aacggtattc agcatccaga actccaatgg aagcagaaag caattcatct
     2581 tctctacgta aacccgcctg cacctgtctt tttattatcg attcattttc tttcatctgc
     2641 gttaaactct gttcagctaa taatacacta tttttacttt gctgatgatt ctgccattgg
     2701 gtcagtatct gataatctaa atcctgctct aatattatct gctccaattt aactatttcc
     2761 aggttattaa tcaattttgc tatactcaat tgggaagcct gctcaatttt agcattctcc
     2821 cgcttaaaaa gggctaactc aacctcaatc cctttacttt ttagagtgaa actattctcc
     2881 cgggctttct cacaggaaac attgcaatct atatcgggga aatggggcaa actaacggct
     2941 tcaaccgata tttctgcctc accagataac cccagagcat tttttatctc aattaacagc
     3001 tgctgataat ccagtctggc cttctcatag gagaataagg catcataata ggcattccct
     3061 tgctgcaata aatctaattc aattctgtaa ccagcagcta cctgggcttc tacctctgcc
     3121 agaaccgctt tctccaaatc aatattcttt tctttacgtc taatgtctaa ctctgccagc
     3181 actaagcgaa aataattatc cgccgcctgg ataatcagct ggctcttctg gttctggaaa
     3241 gtgttttgct gctgtaaaat attgatttcc ttctgtttgt taagaatccc tgattgagtt
     3301 aaaagatttt ctgcttccag ttgacggata tccaattctg cattattcaa ctggtaaata
     3361 gcttttttaa aattcagatt gttggaaatg gtcagtgaaa tcatcctgga tatatctaat
     3421 acaactttct cctgacc
//
LOCUS       DAOQWT010000113         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_28850_length_3428_cov_2.281352, whole genome shotgun sequence.
ACCESSION   DAOQWT010000113 DAOQWT010000000
VERSION     DAOQWT010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28850_length_3428_cov_2.281352"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PKJ95_03060"
     CDS             complement(<1..201)
                     /locus_tag="PKJ95_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR65257.1"
                     /translation="MNKSRKKNRLLLINPWIYDFTAYDFWSKPLGILYVAAILRKKGF
                     DIDFIDCMDRLDTSLFKKTGNIP"
     gene            476..1810
                     /gene="trkA"
                     /locus_tag="PKJ95_03065"
     CDS             476..1810
                     /gene="trkA"
                     /locus_tag="PKJ95_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNR65258.1"
                     /translation="MKIIIIGAGKVGLQIAQTLSRENHDITIIDKREDIREDIDSNLD
                     IMTIVGNGASVRILEQAGIKEADMLIAVTRNDEVNMIACMTAKQFNVAKTIARIRNSE
                     YMYANSISKEKLGIDYVINPEKATAKEIVRLLKTPSNICEVYDFAEGKISLFGLKIVE
                     DSNFANKKLKEISFGQHALMVAIFRENNLIIPTGNDKIMVGDKIYFLIKKESYSDLEL
                     LCDKKNGPLQNVLIFGGSNIGIQVASLLNKIGIKTKLIEINKQKSETISELLPQTLVI
                     NGDGTNTELLKEEGIEKTDGFVAVTGYDEDNLLVSLLAKHFGTKKVIAKISRINYIPI
                     LEKIGIDAIVNPRLTTASAILRFLKSGDLIALTLLKEGELEVAELIAKKDSKIINKPL
                     KLTNLPNNSIIGAIIRDDEVIIPGGDDIIIEGDKVILFTKTTEIKKIEHLFM"
     gene            1827..3272
                     /locus_tag="PKJ95_03070"
     CDS             1827..3272
                     /locus_tag="PKJ95_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNR65259.1"
                     /translation="MNYNVVLHTLGILLSFLGLSMIFPLSYAFYYQEERAIYALLSSI
                     IITILSGLFLRFTFKSEGGIGRKEGFAIVTLGWIIAAAFGTFPFLFSGALNNFIDAYF
                     ESMSGFTTTGATVLQSIEDNPLSILFWRNQIQWMGGMGIIMLVVAILPTLGVGGMQLF
                     KSEVPGPEPDKLKPRIAETAKILYLVYITISALQIACLYFTGMSLYDAIIHMFGTMAT
                     GGFSSKNLSVGHYNNLTFEVIITFFMLIAGTNFTLLYKVLHGDIKSLFKDREFIFYIG
                     LVFISILIIATQLHIYIYKSILTALRYASFQVVSIVTTTGFVTIDFDQWPALSKSILI
                     ILMFIGGCAGSTGGGIKSIRILILIKKVGREFQKILHPHAITPIYIGEKRISDEAVST
                     VTSFFLLYIMIFILGSLVMAALGLDMISAMTSVATTLGNVGPGLGLVGPTQNFAFLPS
                     AGKLFLSFCMLLGRLEIDTVLILLIPEFWKK"
BASE COUNT         1140 a          539 c          585 g         1164 t
ORIGIN      
        1 aggaatatta cctgtttttt taaataagct tgtatctaaa cgatccatac agtctataaa
       61 atctatatca aaacctttct ttcttaagat agctgcaaca tatagtatgc ccaacggttt
      121 ggaccaaaaa tcatatgctg tgaagtcgta tatccagggg ttaattaaaa gtaatctatt
      181 tttttttcta ctcttattca ctccaaaacc taaaaaatcc tttctattaa ctgtttatat
      241 aatagcattt ttcctgtaac aaacaatatc ccttcctctt tagcaacgcg taagactagt
      301 tggccaaaaa ccatacagat actcttacca attataatta tttacactca atgcaacgtt
      361 tgacaaaagc tataaaaggg tatacaattc ccaaggcaaa tgagtgcatt ataaacaatt
      421 tgttttttat taaaactata gttttaaaaa gctatggctt ttttaggagg aacttttgaa
      481 aattattatc attggagcag gaaaagtagg tcttcaaatt gcccagacgc tctcaaggga
      541 aaatcatgat ataacaatca tagacaagcg agaggatatt agggaagata tagatagcaa
      601 tctggatatt atgaccattg tgggaaatgg cgccagtgtc aggattctgg aacaggctgg
      661 tataaaagaa gcagacatgc taattgcagt aaccagaaat gatgaggtca atatgattgc
      721 ctgtatgact gcgaaacagt tcaatgttgc taaaactatc gcccgtatca gaaactctga
      781 atatatgtat gcaaattcaa tttccaagga aaaattagga attgattatg ttattaatcc
      841 cgaaaaagca actgccaaag aaatagtaag actattgaaa accccttcca acatctgtga
      901 agtttatgat tttgctgagg gaaaaatttc tttgtttggt ttaaaaattg tagaagattc
      961 taattttgcc aataaaaaat taaaggaaat aagttttggg caacacgccc taatggttgc
     1021 catatttaga gaaaataatc tgataattcc taccggtaat gacaaaatta tggtaggaga
     1081 taaaatatat tttctaatca agaaagaaag ctattcagat ttagagctat tatgtgataa
     1141 aaaaaatggt cctttacaaa atgttctcat ttttggtgga agcaacattg gtattcaggt
     1201 tgcctctctt ttgaataaaa taggtataaa aacaaaactg attgaaataa ataagcaaaa
     1261 aagcgaaacg atatcagaat tattacctca aactctagtt ataaatggag atggtaccaa
     1321 cacagaactt ctcaaagaag aaggtattga aaagactgac ggttttgtag ctgttactgg
     1381 ttatgatgaa gataatttac tggtttcact gcttgctaag cattttggga caaaaaaagt
     1441 catagccaaa atcagcagga ttaattatat ccctatttta gagaaaattg gtatagatgc
     1501 gattgttaac cctcgtttaa ctaccgcttc tgccatactt cgtttcctga aaagtggcga
     1561 tcttattgct ctaactcttc taaaagaagg agaattggaa gtagcagagc tgattgccaa
     1621 aaaagatagt aaaattatca ataaaccttt aaaattaacc aatttaccca ataattctat
     1681 tattggtgcc ataattagag acgatgaagt cattattccc ggtggtgatg atattatcat
     1741 tgagggtgat aaagttatcc tgttcaccaa aacaacagaa ataaaaaaga tagaacatct
     1801 ttttatgtaa agtggagttt aattcattga attataacgt tgttttacat acacttggta
     1861 tactattatc ctttctaggc ttaagcatga tatttcctct ttcttatgct ttttattacc
     1921 aggaagaaag ggcaatttat gctcttttat cttcaataat aattaccatt ctttctggat
     1981 tgttcttacg tttcaccttt aaaagtgaag gtgggatagg cagaaaagaa ggttttgcca
     2041 ttgttacact gggatggatt atagcagctg catttggtac ttttcccttc ctttttagtg
     2101 gtgcattaaa caattttata gatgcttatt ttgaatcaat gtcaggtttt accactactg
     2161 gagcgacagt tcttcaatct attgaagata atcccctttc catacttttt tggcgaaatc
     2221 aaatacaatg gatgggagga atgggaatta tcatgctggt agtagccatt ttacccacat
     2281 tgggagtagg gggtatgcaa ttattcaaat cagaagtccc cggcccagaa ccagacaaat
     2341 taaaaccccg tattgcagaa acagcaaaga tattatacct ggtttatatt accatttctg
     2401 ctcttcagat tgcctgtctt tattttactg gaatgtcttt atatgatgcg attatacata
     2461 tgtttggcac tatggctacc gggggttttt cttcaaaaaa tctaagcgtt ggtcattata
     2521 ataatctcac ttttgaggta ataattactt tttttatgtt aatagctggt acaaatttta
     2581 ctctgctcta taaggtactt cacggtgata taaaaagcct gtttaaagat agagaattta
     2641 ttttttatat tggtttagtt ttcatttcca ttctaattat tgctactcaa ttacacatct
     2701 atatatataa atcaatatta actgcactcc gttatgcttc ctttcaagtg gtttctatcg
     2761 tgaccacaac tggttttgtc accattgatt ttgaccaatg gcctgcatta tccaaaagta
     2821 ttctgataat tttaatgttt ataggaggtt gcgctggctc tactggtggc ggtataaaaa
     2881 gtattcgcat tcttatttta ataaaaaaag taggtcggga gtttcaaaaa atccttcatc
     2941 cacatgccat cactccaatc tatatcggtg agaaaagaat ctctgatgag gcagtgagca
     3001 ctgttacctc ttttttctta ttgtatataa tgatatttat cttaggttct cttgttatgg
     3061 ctgctttggg attagatatg ataagtgcta tgacttcggt agcaacgact ttaggaaatg
     3121 ttgggccggg attaggactg gttggaccca ctcaaaactt tgcttttctt ccttcagcag
     3181 gaaaactatt tttaagtttt tgtatgcttt taggacgatt ggaaattgat actgtactaa
     3241 tccttcttat tcctgaattc tggaaaaaat aaatttctat tctatctaac taaatgctta
     3301 aatattgtat ttgttgagag cttcataaaa tttatggtat aatatttggt acaacaataa
     3361 ggttaattaa aaaaatattg aattttcata tataaaagat aattaacaaa gttaatcact
     3421 ttagattt
//
LOCUS       DAOQWT010000114         3417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_28982_length_3417_cov_3.357228, whole genome shotgun sequence.
ACCESSION   DAOQWT010000114 DAOQWT010000000
VERSION     DAOQWT010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28982_length_3417_cov_3.357228"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="PKJ95_03075"
     CDS             complement(<1..1311)
                     /locus_tag="PKJ95_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNR65260.1"
                     /translation="MLTRLEIMDTTLRDGEQMKNVSYSPDEKLTLSKILLEEIRVDRI
                     EVTSAKVSKGENDAVRKIVEWANRNRYGEKIEVLGFVDIHESADWISKLEGKVINILS
                     KGSLKHLTTQLKKTKEQHLKEIKETIRYANKKGLICNIWFEDWSNGMIDSPDYVYFLL
                     DHLREENIKRFMLPDTLGILYPSQVYNFIKEIILKYPGLHFDFHGHNDYGLATANTIA
                     AAEAGVKGVHCTANGMGERAGNAALDEVVVGIRDFLKIETTVDEKKLFNLSKTVEAFS
                     TYRIAANKPISGENVFTQTAGIHADGDRKGNLYTSKLLPERFNRKRQYSLGKLSGKSN
                     LDFNLEEIGLQISKKQKMDVLNKIVELSDKKKTITTADLPYIVSDVLNTPEENIFKLK
                     AYNILTSHSTRPVATIKMLYKTNGNPDFIELEESAQGNGGYDAFM"
     gene            complement(1338..2864)
                     /locus_tag="PKJ95_03080"
     CDS             complement(1338..2864)
                     /locus_tag="PKJ95_03080"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNR65261.1"
                     /translation="MTDLIRIFDTTLRDGEQCPGASLNTQEKLEIARHLEKMRVDVIE
                     AGFPISSPGDFEGVETIAKNIKGPIIAALCRAVEQDIKCAWDAIKLAQKPRIHTFIAT
                     SPIHMKLKLEKTPQEVLEQAVSAVRLAKSYCQDVEFSAEDASRSNPEFLYQIFTKTIE
                     AGATVINIPDTVGYSQPEEFASLIKGIKANVPNINKVTMSVHCHNDLGLAVANSLESL
                     KYGVRQIECTINGIGERAGNASLEEIVMALYTRKDFYQKYTNIDTGYIYPTSKLVSRL
                     TGFVVQPNKAIVGKNAFAHEAGIHQAGVLKDASTYEIMRPQTIGLESNQLVLGKHSGR
                     NAFKSRLAQLGYDLDKEKLDKSFEQFKVLADKKKEIYSEDLLALMSEVLVEEIADIYE
                     LSYFHISTGNNILPTATVKLIREGNVFEDAACGDGPVDAVFNAIDRITGVKVKLSDYN
                     IRALTRGKDAQGEAVVEITDNGCSFIGKSASTDTLEASTKAYLKAINKMLLRNSYKDK
                     "
     gene            complement(2945..>3417)
                     /gene="ilvN"
                     /locus_tag="PKJ95_03085"
     CDS             complement(2945..>3417)
                     /gene="ilvN"
                     /locus_tag="PKJ95_03085"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNR65262.1"
                     /translation="EDLPGVMARVASLFTRRGFNIDSIAVGHSETPGVSRMTIIVNGD
                     EQVLEQIMKQLNKLIDVIRVRDIPSDSIIERELVLVRVHVESLTVRSEIIQLVEIFKA
                     KVVYVERNALTIEMSGDEEKINGLLNLLKPFGIREIVRTGMIAINKNSKNGLKP"
BASE COUNT          980 a          741 c          458 g         1238 t
ORIGIN      
        1 cataaaagca tcatatccgc catttccttg tgcactttct tccagttcaa taaaatctgg
       61 gttcccattc gttttataaa gcatttttat agtagcaaca ggtctggtag aatgactggt
      121 caaaatattg tatgctttaa gcttaaatat attttcttct ggagtattta atacatctga
      181 aacaatatag ggcaaatcag cagtggtgat agtctttttt ttgtcactca actcaactat
      241 tttgttcaaa acatccattt tttgtttttt agatatttgt aaaccaatct cttctagatt
      301 aaaatccaaa tttgatttac cactcaactt ccctaaagaa tattgccttt ttctattaaa
      361 cctctctggc aataatttac ttgtataaag attacccttc ctatctccat ctgcatggat
      421 accagctgtc tgggtaaata cattttctcc actaattggc ttatttgctg caatccgata
      481 agtagaaaat gcttccactg ttttactgag attaaacaac tttttctcat caacagtggt
      541 ttcaatcttt aaaaaatctc taataccaac tactacttca tccagggcag cattcccagc
      601 cctttcaccc attccatttg ctgtgcagtg aactcccttt actcccgctt cagctgcagc
      661 gattgtattt gctgtagcca gcccataatc attgtgacca tggaaatcga aatgtaaacc
      721 aggatatttt aatattatct cttttataaa attataaacc tgggaaggat atagaattcc
      781 cagcgtatct ggtagcataa acctctttat attttcttca cgaagatggt ccagtaaaaa
      841 atatacataa tcaggtgaat caatcatacc gttagaccaa tcttcaaacc aaatattaca
      901 aataagacct tttttatttg cataccttat tgtttcttta atttctttaa gatgttgttc
      961 ttttgttttt ttcaattgag ttgttaaatg cttcaatgac cccttactga gaatgtttat
     1021 tactttccct tctagtttag aaatccagtc tgcagattca tggatatcca caaatccaag
     1081 cacttcaatt ttttctccat atctatttct gttcgcccac tcaacaattt tcctgacagc
     1141 atcgttttcc cctttgctaa cttttgcact tgttacctca attctatcta ctcttatttc
     1201 ctccagtaat atttttgaca atgtaagttt ttcatcaggg gaataactta cattcttcat
     1261 ttgttctcca tctcttaatg ttgtatccat tatttctaat ctggttaaca atttactacc
     1321 ctcttcttat attcatttta tttatcttta taactgtttc gtaacaacat tttatttatt
     1381 gcctttaagt aagcctttgt gctggcttcc aaagtatcgg tactggcact tttccctata
     1441 aatgaacaac cattatctgt tatctctacc actgcttcac cttgagcatc tttccctctg
     1501 gttaaagccc tgatattgta atcagaaagt tttactttta ctccggtaat tcggtcaata
     1561 gcattaaaaa cagcatctac cggaccatct ccacaagccg catcttcaaa aacatttcct
     1621 tccctgatca gtttaacagt agcagttggc aggatattat ttccagtact gatgtggaaa
     1681 taacttaatt catagatatc agctatttcc tccactaaaa cttcagacat aagggctagc
     1741 aaatcttcag aatatatctc tttcttctta tcagcaagaa ctttaaactg ttcaaatgat
     1801 ttatctaact tttctttatc cagatcgtaa cccagctgag ctaatctgct tttaaaagca
     1861 tttctccccg aatgttttcc caaaaccagt tgattacttt ctaaaccaat agtttgtggt
     1921 ctcataatct cataagttga agcatctttt aaaactccag cctggtgtat accagcctca
     1981 tgggcaaaag cattcttacc cacaattgct ttattgggct gcactacaaa acctgttaag
     2041 cgactgacca atttactggt aggatagata taaccagtat ctatattggt atatttttgg
     2101 taaaaatcct ttctggtata aagggccata acaatctctt ccagtgaggc attacctgct
     2161 ctctctccta ttccatttat agtacattca atctgtctca caccatattt taaggattcc
     2221 agagaatttg ccacagctaa gcccagatca ttgtgacagt ggacactcat tgtcacctta
     2281 ttaatatttg gcacatttgc tttaattccc ttgattaaac tggcaaactc ttctggctga
     2341 gaataaccta cagtatctgg tatatttatt actgtagccc ctgcttcaat tgttttggta
     2401 aatatctgat ataaaaactc cgggttgcta cggctagcat cttcagcaga aaattctaca
     2461 tcctggcaat aactctttgc caaacgtacg gcagatactg cttgctctaa tacttcttga
     2521 ggtgtcttct ccagtttcag cttcatatga attggagagg tggcaataaa agtgtgaatt
     2581 ctcggttttt gagccaattt aatagcatcc caggcacatt taatatcctg ttccactgcc
     2641 cgacacaatg cagcaataat aggtcctttg atatttttgg caatagtttc aacaccttca
     2701 aaatctcctg gtgaggatat cgggaaacca gcctcgataa catcaactct cattttttct
     2761 aaatgtctgg caatctctaa tttttcctga gtatttaaag acgctcccgg acattgctct
     2821 ccgtcacgca aagttgtgtc aaatattctg attaaatctg tcataaataa cctcctttaa
     2881 tcaatttata atggaactta ttaatttatt ttaattattt tttctgtatt attcgaaata
     2941 ttatttacgg ttttagacca ttcttactat ttttatttat ggcaatcatt cccgtccgaa
     3001 cgatttctct aattccaaaa ggttttaaca aattaagtaa accgtttatc ttttcttcat
     3061 cacctgacat ttcaatagtt aaggcattcc tttccacata aactactttt gccttgaata
     3121 tttccactaa ttgaataatt tctgaacgaa ctgttaatga ttccacatgc actctgacta
     3181 aaaccagttc tctctcaata atgctgtctg aaggaatatc tcttactctt atcacatcaa
     3241 ttagtttatt taattgtttc ataatctgtt ctaaaacttg ttcatcacca ttgacgataa
     3301 ttgtcattct ggaaactcca ggtgtttccg aatggccaac ggcaatacta tcaatattaa
     3361 aacctctcct ggtaaataag ctagctactc tagccattac tccgggcaaa tcttcta
//
LOCUS       DAOQWT010000115         3406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_29109_length_3406_cov_3.279320, whole genome shotgun sequence.
ACCESSION   DAOQWT010000115 DAOQWT010000000
VERSION     DAOQWT010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29109_length_3406_cov_3.279320"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..880)
                     /locus_tag="PKJ95_03090"
     CDS             complement(257..880)
                     /locus_tag="PKJ95_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65263.1"
                     /translation="MVKDEKQIKEMVRIRSFFYLLFLILYLIPIAIVLIDGFDNLLQF
                     IRRIVGMAGLSSLFITILLSLLIKESKKIFGINYVKIHHFFSMASVILISIHPVIAAI
                     SFGTITIFSPKLDSWNVFLTNAGCPALYLIYFAVITALFRKSILKYWKYFHYLLYPAF
                     LLSAIHGILKGSDSDNRMMFFLVLVMTSVVTIIFFYKRFQNIRKGPG"
     gene            complement(1019..2287)
                     /locus_tag="PKJ95_03095"
     CDS             complement(1019..2287)
                     /locus_tag="PKJ95_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNR65264.1"
                     /translation="MMRKEEKINYDVAVVGGGPAGMISAGRAAELGAKVVLLEKNDSL
                     GKKLLITGKGRCNFTHYEFDRDKLAEKFGKEGRFLYSALDHFGVREVLNFFQFRGLTW
                     QVERGNRVFPKEGNAQDVLRILTGYLREGGVNFFCNSRVSDLEIIQNGSEKFRIIYSG
                     RELFSKRVILSTGGKSYPQTGSNGDGYHWAEKFGHTIINPEPSLNPVETAEKWVKEVQ
                     GLTLKNVSLQLYLEGRKRDERFGEMLFTHFGISGPIVMDMSKSIAQHLKKGEVKLVLD
                     LKPSLDFQKLEERIQRDFQEFKGRMFKNSLQKILPSSLIPVIIRLSAIEEEKKVDYIT
                     KEERKRLVHLLKELELTPTELLGFPWAMVTSGGVSLREVNPDTMASKKIKNLFFAGEI
                     LNLDGPSGGYNLQECWSTGYLAGESAAGKN"
     gene            complement(2342..>3406)
                     /locus_tag="PKJ95_03100"
     CDS             complement(2342..>3406)
                     /locus_tag="PKJ95_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR65265.1"
                     /translation="QYDKLVFAIGSLPIIPDIPGINKKNVFPVRKEFDYLRDVLNKVR
                     EAKNVVIIGGGFIGVEFADECRKNPGVKVTVVEMQEYCLRIAMDDEFCNDAEKKLGEA
                     GVKIYKNEQVIAIHGNDYVEEVELSSGEKIKADVVILGIGTVPNSKLAEESGIELGYR
                     GGIRVDRFMRTFTDRDIFACGDCCTKESFFDGKPSLVMLASVATAEARIAGANLFSPV
                     HHNSGVISVFATIINDRAFGVAGLTERAAKANGMNVVIGEASAPDTHPGGMPNSTMLK
                     VKLMFGKNNGIILGGAASGGKSTGEMLNVISACISHRMTANDIVLFQMGTHPALTSSP
                     IAYPIVNAACKAVLPLSGCV"
BASE COUNT         1049 a          760 c          529 g         1068 t
ORIGIN      
        1 gttttattgg gaaccttaag ataataatta tagatttttc tcagtctcca ttgaacaagc
       61 catcctaaaa ataccggaac aactagcaac aagtagaccg gctgaaaggt aagcattaga
      121 ctacctacac ctctctttat aagtttaatg aaaaaattct gatattagta ctggcttttt
      181 cttaatcatt tttggataaa aacatagcac ctgccacttt agaaaccatt tattaataac
      241 tcaaaattgg taaatactat cccggaccct tcctgatatt ttgaaacctt ttatagaaaa
      301 agatgatggt aacaacagaa gtcattacaa ggacaagaaa aaacatcatt ctgttatcag
      361 aatcgctgcc ctttaaaata ccatgaattg ccgacagaag aaaagccgga taaagcaggt
      421 aatgaaaata tttccagtat tttaatatac tcttcctaaa aagagctgtt attactgcaa
      481 aataaattag atacaaagca gggcagccgg cattagtgag gaaaacattc caggagtcta
      541 attttggaga aaatattgtg atagttccaa agcttattgc ggcaatcacg ggatgtatac
      601 taattagtat tacactggcc attgagaaaa aatgatgtat cttcacataa tttattccaa
      661 aaattttttt actctctttt atcaataaac ttaggaggat tgtaataaaa agagaggaga
      721 gaccggccat accaacaata cggcggataa attgtaacag gttatcaaat ccgtctatca
      781 gaacaatggc aataggaatt aaatagagaa taagaaataa cagatagaaa aaagaacgaa
      841 ttctaaccat ttctttaatc tgcttttcat cttttaccat actctactcc ttttactttt
      901 aaaatcactg aaacataata gttagtgcct aacaattgca gggatttaaa atctattagc
      961 agaaaaagta tgaacagata gttagattaa cagaacaatt agggtgctaa atatatattt
     1021 aattttttcc agcagcactc tctccggcta aataaccggt actccaacat tcttgtagat
     1081 tatatccccc tgaaggacca tctaaattca ggatttctcc ggcaaaaaaa agatttttta
     1141 tctttttaga agccatagta tcaggattaa cctctcttaa agatactcct cctgaagtta
     1201 ccattgccca ggggaatccc agtaattcgg ttggtgtcaa ctccagttct tttaagagat
     1261 gcactaatct ctttctttcc tctttggtaa tatagtccac cttcttctcc tcttctatag
     1321 cagacaatct tataattacc gggataaggg aggaaggtaa tattttttgt aaagaatttt
     1381 taaacatacg tcctttaaat tcttgaaaat ctctttgaat tctctcttct aacttttgaa
     1441 aatccaaaga gggttttaaa tccagcacta atttgacttc cccctttttt agatgttgcg
     1501 ctatactttt gctcatatcc atcacaattg gtccgctaat accaaaatgg gtaaataaca
     1561 tctcgccaaa tcgttcatca cgctttctcc cttccaggta caattgcagt gaaacattct
     1621 ttaaagtcaa gccctgtacc tcctttaccc atttttcagc tgtttccacc gggtttaagg
     1681 atggctctgg attaataatg gtatgcccaa acttttctgc ccaatggtaa ccatcaccgt
     1741 ttgaaccggt ctggggataa gatttaccgc cggtagagag aataactctt tttgaaaata
     1801 attcccttcc ggaataaatt attctaaatt tttctgaacc attttggatt atctctaaat
     1861 cgctgaccct ggaattacaa aaaaaattta ctcccccttc tcttaaataa ccggttaata
     1921 tcctaagcac atcctgagca ttcccttctt ttggaaaaac ccgattaccg cgctctacct
     1981 gccaggtaag tccgcgaaat tgaaaaaagt tcaatacttc tctcactcca aaatggtcca
     2041 gtgcactata cagaaaacgc ccctctttgc caaacttttc agctaattta tctctatcaa
     2101 attcataatg ggtaaaatta catcgtcctt ttccagtaat cagcaatttt ttccccagag
     2161 aatcgttttt ttccaacaag actaccttag ctcccagctc agctgcccga ccggctgaaa
     2221 tcataccggc agggccgcct cctaccactg ccacatcata attaatcttc tcttctttcc
     2281 tcatcattgt ttattgttgg cttatattta gcgcttaatc cttttcttta taaaattctc
     2341 tctaaacaca gccactgagg ggtagaacag ctttgcaggc tgcattaacg ataggatagg
     2401 caataggtga tgaagtcaat gcgggatggg tgcccatctg aaataagaca atatcattgg
     2461 ctgtcattct gtgagagata caggcactga tgacattgag catttcaccc gttgattttc
     2521 cgccggaggc cgctccgccc agaatgattc cattattttt accaaacatc agttttactt
     2581 tcagcatagt ggaattgggc attcctccgg gatgagtatc aggagcagag gcttccccta
     2641 tcacaacatt cattccattt gccttggctg ctctttccgt taatccagcc acaccaaagg
     2701 ccctgtcatt gataattgtg gcaaatacac ttattacacc gctgttatgg tgtacaggag
     2761 aaaacaggtt ggcaccggca atgcgtgctt cagccgttgc cactgatgcc agcattacta
     2821 atgatggttt cccgtcaaaa aaagattctt tagtacaaca gtcaccgcag gcaaatatat
     2881 ctctgtctgt aaatgttctc ataaaacgat caaccctgat tccaccccta tatcccaact
     2941 ctatacctga ttcttccgct aatttgctat tgggaacagt gccaattccc agtataacta
     3001 catctgcttt tatcttttct ccactggata attctacctc ttccacataa tcatttccgt
     3061 ggattgcaat tacctgctca tttttatata ttttaactcc tgcctcgccc aattttttct
     3121 ctgcatcgtt acaaaattca tcatccattg ctattctcag gcagtattcc tgcatctcaa
     3181 ccacggtcac tttgaccccg ggatttttcc ggcattcatc agcaaattct accccaataa
     3241 atcctccccc tataattacc acatttttcg cttcacgaac cttgttaaga acatctctga
     3301 gataatcaaa ttcttttctg acaggaaaga cattcttctt attaattcca ggtatatcag
     3361 gtataatagg caaagaaccg atagcaaaga ctaatttatc gtattg
//
LOCUS       DAOQWT010000116         3403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_29145_length_3403_cov_3.985066, whole genome shotgun sequence.
ACCESSION   DAOQWT010000116 DAOQWT010000000
VERSION     DAOQWT010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29145_length_3403_cov_3.985066"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1647)
                     /locus_tag="PKJ95_03105"
     CDS             complement(<1..1647)
                     /locus_tag="PKJ95_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65266.1"
                     /translation="MQKYKYALDLEENIYSLNSDFDTYKLSSDEIKKIRSEPISDKDS
                     RYIDRKIVYDHTYIYLTPGEFIEPYKLRNNQKIELYDDIGKIKIKDITPENIKDISFI
                     LHHILMIRAQEDDEVHGYEMSISSSWQYIPFNQRMNFSIENIFNKQDKAINTQKELNN
                     RISSSNEDKLLLSEEATNILKYQENKVLNGFYSGLPNFTHNERKNNEVKIAQNELFNN
                     FTGGKKDSSEAKNNTEKYPMPPDPIRDLEDYVKIPEELRKNFPYNFSPMSSKKIPTEA
                     LNDINVNIDYIKQSANMTNIDPAFLTGILFSERLRIDYIQDYIGDHFSGETKGFSQIS
                     IASALEGQRHHCEKHNIPFSEAEEKKDIEKNIFSDKNWSDAKNIEYAAKYIDFLRDQF
                     PLSHNLTQEDQNRLVAAAYKMGPETIKTARMQEQLNDLANTNLKTDGYYGNNTNKIIY
                     DILTSNKELLNKNDRFAKDIERLTPPDTGDTNYKDLYHQSDYEVIRTMVNTLWARKHP
                     GEYNPPGAVPNNQDKLPEERVDYHLQDVFEGMKLYNNNLFN"
     gene            1717..1869
                     /locus_tag="PKJ95_03110"
     CDS             1717..1869
                     /locus_tag="PKJ95_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65267.1"
                     /translation="MVKYILSVQYLLSMPGFAFSTLRISAACYLTVYFPGFPFVEARH
                     DRGLKG"
     gene            2311..2580
                     /locus_tag="PKJ95_03115"
     CDS             2311..2580
                     /locus_tag="PKJ95_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNR65268.1"
                     /translation="MLKTISAMKARQNLGQIMNEVSIRSDSYIIERNGKPLVAIVPVS
                     FLEEQELKKKGFLNLLDKAQSNSNVTEEDIQNDIREAIKEVRKNK"
     gene            2580..3008
                     /locus_tag="PKJ95_03120"
     CDS             2580..3008
                     /locus_tag="PKJ95_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNR65269.1"
                     /translation="MIRAVLDTNVLVSGLLSSGGICNQIIRKWITGEFILCICTEALE
                     ELEKVVRYPNIAKRHKLSEDEIKQYIEYAEEFAFNVNVTEIEDVVKTDPSDNIFPACA
                     LAGGADYIVSGNYHLLDLKSYQSIKIITPRDFYDILITRR"
     gene            3099..>3403
                     /locus_tag="PKJ95_03125"
     CDS             3099..>3403
                     /locus_tag="PKJ95_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65270.1"
                     /translation="MESEKIEIKKWQGFQDYYRLSTSPKTLKEYSAIIKLIEEGKLEI
                     KSKSRYLQALAAIKKAGRAGIDIDYMPEWHKREKRRVKTVQDKLITREQLKQILQSVP
                     "
BASE COUNT         1082 a          524 c          591 g         1206 t
ORIGIN      
        1 attaaacaag ttattattat agagtttcat tccttcaaaa acatcctgga gatgataatc
       61 cactctttct tcaggtaatt tatcttgatt attcggaaca gcaccaggag gattgtattc
      121 acctggatgt tttcgagccc ataaagtatt taccattgtt cttattacct cataatcact
      181 ttggtgatac aaatctttat aattcgtatc tcctgtgtcc ggaggagtta atctttctat
      241 atccttagca aatctatcat tcttgtttaa taactctttg ttggatgtta atatatcgta
      301 gataatttta ttagtattgt taccataata accgtctgtt tttaaattgg tatttgcaag
      361 gtcatttagt tgttcttgca ttcttgcagt ttttatagtt tctggtccca ttttataagc
      421 tgctgctact aatctatttt gatcttcttg tgttaaattg tgactcaaag gaaattgatc
      481 tcttaaaaaa tctatatatt tagcggcata ctcaatattt ttagcatcac tccagttttt
      541 atctgaaaaa atgtttttct ctatgtcttt tttttcctca gcttcactaa aaggaatatt
      601 atgtttttca caatgatgtc tctgaccttc taaagctgaa gcaatactta tctgagaaaa
      661 cccttttgtt tctccactaa aatgatcacc tatatagtct tgaatatagt caattcttaa
      721 tcgttcagaa aacaatatac ctgttaaaaa ggcagggtct atatttgtca tatttgctga
      781 ttgtttaata taatctatat ttacatttat atcgtttaaa gcttctgtag gtattttttt
      841 tgaactcata ggtgaaaaat tataaggaaa attttttctt aattcctctg gaattttaac
      901 gtaatcttca agatctctta taggatctgg aggcatagga tatttttctg tattattttt
      961 tgcttctgaa ctatcttttt ttcctccagt aaagttatta aatagctcat tttgtgctat
     1021 ctttacttcg ttgttttttc tttcattatg tgtaaaatta ggtaatcctg agtaaaaacc
     1081 atttaaaacc ttattttctt gatattttaa aatatttgta gcttcctcag ataaaagcaa
     1141 tttgtcttca ttacttgaag atatgcggtt atttaattct ttttgtgtat ttatagcttt
     1201 atcctgtttg ttaaaaatat tttcaatact aaaattcatt ctctgattga aagggatgta
     1261 ttgccaggag ctactaatcg acatttcata accatgaact tcatcatctt cttgagctct
     1321 tatcatcaaa atatggtgaa gtataaaaga aatatcttta atattttctg gagtaatatc
     1381 tttaatttta atctttccta tatcatcata aagttctatc ttttgattat tacgcaattt
     1441 atacggttca ataaactctc ctggtgttag gtaaatataa gtatgatcat agacaatttt
     1501 tctatcgata tacctactat ccttatctga tataggctca gaacgtattt tttttatttc
     1561 atcactacta agtttgtaag tatcaaaatc cgaatttaag gagtaaatat tctcttctaa
     1621 atcaagagca tatttatatt tttgcatgaa tagaatcctt tctctgttta ttaaattata
     1681 aactctttat ttacgagtat ataaaactat tacagtatgg tcaagtatat cttatctgtg
     1741 caatatcttt tatctatgcc tggatttgct ttttcaactc tgaggataag tgcagcttgc
     1801 tacctcactg tttatttccc tggcttcccc ttcgtagagg cccgccatga cagagggttg
     1861 aaagggtaga ctgcaaattt aattagtcgg gctaacatgg ggcaggtgaa aaatcaaatc
     1921 aggggcgggc tgcacgcact gacaattaat tgcaaagctc agtaaaaatc tggcagaacg
     1981 gttttaaagg gcggggaggg gggcggctac aacggcctgc tgcactgcac tgcttatgct
     2041 cttttgcatg cgtgctatgt gcaagctctg catactgcag ctcctatttt tgcaaagttc
     2101 ggatctgttt tctccggatt ttatcggccc ggtgcaatca agctatacct gcttgatttg
     2161 ccgggtctat ctgcaaattg ccggttaaat cggaaaataa aattacaccg gctgcggcaa
     2221 tcaaaaatgt accaacattt atcttttaaa atatgatata attatattga ttgatataat
     2281 taatatgatt atattgggag gataattaag atgcttaaaa caataagtgc tatgaaagca
     2341 aggcaaaacc tgggacagat tatgaatgaa gtaagtataa gaagcgacag ttatattatt
     2401 gaacggaacg gtaaaccact ggttgccatt gttcctgtat catttctgga agaacaggaa
     2461 ttaaagaaaa aaggctttct taatttactg gataaagctc agagtaattc taatgtaaca
     2521 gaagaagata ttcagaatga tataagggaa gcaattaaag aagtgaggaa gaataaataa
     2581 tgatcagagc cgttctggat accaatgttt tagtgagtgg tcttctgtct tcgggcggaa
     2641 tctgtaatca gattataaga aaatggatta ccggagaatt tattctttgc atctgtacag
     2701 aagctttaga agaactggag aaagtggtca gatatccgaa tatagcaaaa agacataagt
     2761 tatctgaaga tgaaataaaa cagtatattg aatatgcaga agaatttgct ttcaacgtaa
     2821 atgtaaccga aatagaggat gtggtaaaaa cagatccatc ggataatatt tttcctgcct
     2881 gtgcacttgc aggaggtgca gattatatcg tgagcggtaa ttatcatctc cttgacctga
     2941 aatcatatca aagtattaaa attataactc ccagagattt ttatgacatc ctgattacaa
     3001 ggaggtaggt tttttttaga aaaattttat ctttttaatt ttttctgcta taatatgact
     3061 aactaatata tgattagttc ttcataggag atgatattat ggaaagtgaa aaaatcgaga
     3121 tcaagaaatg gcaagggttt caggattatt acagattaag tacgagtcca aagacattaa
     3181 aagaatattc agctattatt aaattaatcg aagaggggaa actggagata aaatctaaga
     3241 gcagatatct tcaggcactg gctgctataa aaaaggccgg gagggccggt atagacatag
     3301 attacatgcc ggaatggcat aaaagggaga aaagaagagt gaaaactgtt caggataaac
     3361 ttattaccag agaacagtta aagcagattc ttcagagtgt tcc
//
LOCUS       DAOQWT010000117         3403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_29152_length_3403_cov_2.947431, whole genome shotgun sequence.
ACCESSION   DAOQWT010000117 DAOQWT010000000
VERSION     DAOQWT010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29152_length_3403_cov_2.947431"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKJ95_03130"
     CDS             complement(<1..249)
                     /locus_tag="PKJ95_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65271.1"
                     /translation="MNRLKIKKMLVIFLSSMILISLVNVSLATNEKYLVKSELTGWEE
                     VDTYHEIIAYFEILAEDFDQVKMIEMGPTDSGYPLHLVM"
     gene            complement(1103..1759)
                     /locus_tag="PKJ95_03135"
     CDS             complement(1103..1759)
                     /locus_tag="PKJ95_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNR65272.1"
                     /translation="MIVSTLYVGGCNFRCPFCYNADLVINPEKFPVIPEDYLFNYFWE
                     RKNFLDGVCLSGGEPTIYDDLPEFLSRIKNCNLKIKLDTNGSNPDKLSRIIKGGFVDY
                     IAMDIKSCLETEVYQRAIGVQSTQMVTNIRESINMISNSGIDYEFRTTIVPIFHNEIT
                     IEKIAREIKGARKYVLQNFVSTEELLDNKLADVKPYSTQKMEELRRIATFYVENCIVR
                     "
     gene            complement(1797..2072)
                     /locus_tag="PKJ95_03140"
     CDS             complement(1797..2072)
                     /locus_tag="PKJ95_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR65273.1"
                     /translation="MLKLKVFWQKDCPNCPKGKEIAKELEEELEVQYCDVETVDGLSE
                     ACMYQVMSTPSLIIVDEKGEEVVSWKGILPEIDYIRERIFNREKNQN"
     gene            complement(2106..>3403)
                     /gene="nrdD"
                     /locus_tag="PKJ95_03145"
     CDS             complement(2106..>3403)
                     /gene="nrdD"
                     /locus_tag="PKJ95_03145"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNR65274.1"
                     /translation="PEHLKEVPAIGPGGKYTGKTYQEYKSESQQFLKALMEVWEAGDS
                     NGKVFAFPKMDLHIDGNSFQDPEEIELLKYACQIASENGTPYFIFDRDGVSLAACCRL
                     KNEITDMDMIKYPEKLRFSGIQNVTINLPQCAYRTLKSIERGKSYSEGEKFDRFFEEI
                     DSALKLAVKAHKEKKVFLQKLMSSPEGPLWQIGKISPDGKPYVDLEKGTYLIGLIGLN
                     EAIHYLTGFQLHESNDAYIRGLKTVSFLHLKCKDYSKKTEILLSLEESPAESAAGRLA
                     KIDLREFPESEKVIKGSRADDDCYYTNSIHFAASADIDLISRIEKQSKFHPLIKSGAI
                     IHAFVGEKKPPADSIYNLICKVWENTAAAQITISPEFSVCNRCQKVSRGLLDFCSHCH
                     SNDVYGITRIVGYYSKIQNWNKNKIGELKDRKSGNYQIT"
BASE COUNT         1055 a          673 c          462 g         1213 t
ORIGIN      
        1 catcactaaa tgcaacggat aaccactatc agttggtccc atttcaatca tctttacctg
       61 gtcaaagtct tcagcaagga tttcaaaata tgcaattatt tcatgatagg tatccacttc
      121 ttcccatcca gttagctctg acttaacaag gtatttttca ttggtagcca aagaaacatt
      181 aacaagacta attaaaatca tacttgaaag aaaaataacc aacattttct ttatttttaa
      241 tctgttcata tttacctccc tttattttaa ttctctaatt tgtaatttga agtttattta
      301 ttgagtaaaa tgttgcctgt cattaacaat ttctaccttt gatatatctc tataaacacc
      361 accccttttc attattttag tatgttaatt ctttgttaga actacacaga atatgattaa
      421 atatctatat gtacttgata gccatcctat ttaattaaat attcaaaaag aacaaatttt
      481 gaataatgtc tgaaagatgc tcgttagaaa tagtaatttt tttgttttca aatacaaata
      541 tttttaatta caattttaca ttattctaat ttggtaatac tgttaaaaac tatttttttc
      601 tatgatccat acactgggtt aagattaaat aaatattgtt aaatatagat tttaatatct
      661 tctctcttca aaaataagag aaaatttttt atcggtataa agtttaaaat gttatgtccc
      721 tgaattctta tggaatatcc ttttttagaa aacattaagg aagtagtatt aatcaagact
      781 gtatcttatt aaaagaaggc aaagaatgga aaatccatta ctctaccttc ttattaataa
      841 tagtattcct gataaaggga gaatctatac ctgaatatgt tattgtgtta caaattttat
      901 attttaacac aacaaaaaac tttccttcca gattttctct ctatttccgg aaaggcagaa
      961 aatacagtgg ctatatttat ttcaaattaa catatttcaa aacctattaa ttataacatt
     1021 acttttctaa aaagccaaat tattctgttt ttattgttat tatttttgat aagtattact
     1081 ggaatggcag tgattaaata acttatctca ctatacaatt ctctacataa aatgttgcaa
     1141 ttctcctcaa ttcttccatt ttttgtgtag aatagggctt aacatcagcc agcttattat
     1201 ctaaaagctc ttcagttgaa acaaaatttt gtaatacata tttccttgct cccttaattt
     1261 cccgggcaat tttttcaatg gtaatttcgt tgtgaaaaat tgggactatc gttgttctaa
     1321 attcataatc aattccggaa ttactaatca tattaatgct ctccctaatg ttggtgacca
     1381 tctgtgtaga ttgaactcca atggctcttt gatatacttc tgtttctaaa caacttttta
     1441 tatccatagc tatataatca acaaaacccc cttttattat ccgggataat ttatctggat
     1501 tactgccatt ggtatcgagt ttaatcttta aattacagtt ttttatcctg gacaagaatt
     1561 cgggtaagtc atcatatata gtgggttctc caccgctgag acatactcca tcaagaaaat
     1621 tctttctttc ccaaaagtag ttaaaaagat agtcttcagg gataactggg aatttttcag
     1681 gattaataac caaatcagca ttgtagcaga aagggcaacg gaaattacac cctcccacat
     1741 agagggttga tacaatcatt ccttcccaat caattaaaga atttgggatt agattcctaa
     1801 ttttgattct tttctctatt gaaaatcctt tctctgatat agtcaatttc aggcaatatc
     1861 cctttccagg atactacttc ttctcccttc tcatcgacaa ttataaggga aggggtgctc
     1921 ataacctgat acatacatgc ctcgctcaaa ccatctaccg tctcaacatc acagtattgc
     1981 acttctaatt cttcctccaa ttcttttgca atttcctttc ccttaggaca atttggacaa
     2041 tccttttgcc agaaaacttt caacttcaac atacactaat actactcctt ttaaactaag
     2101 actctttaag taatctgata attgcctgac tttctatctt ttaattctcc aatcttattc
     2161 ttattccagt tctgtatttt gctataataa ccgacaattc tggtaatacc gtagacatcg
     2221 ttactatggc agtggctgca aaaatccagt aaacccctgc tcactttctg acagcggtta
     2281 cagaccgaaa actccggtga gatggtaatc tgcgcagcag ctgtattttc ccatacttta
     2341 catatcagat tgtaaatact atcagcaggt ggtttttttt cacccacaaa ggcatgaata
     2401 atagcaccgc tcttaatcaa agggtggaac ttactctgtt tttcaatcct gctaataagg
     2461 tcaatatcgg cagaggcagc aaaatggata ctgttagtat aataacagtc atcatctgcc
     2521 ctacttccct ttataacctt ttcgctttct ggaaactccc ttaaatcgat tttagccaat
     2581 cttcccgccg cactttcagc cggtgattct tctaaagata ataatatttc ggttttcttg
     2641 ctataatcct tacatttaag atgtaggaag gaaacggtct tcaatcccct tatataggca
     2701 tcattgcttt catgaagttg aaaaccggtt aaataatgta ttgcctcatt taatccgatt
     2761 aacccaatta gataggttcc tttttccaaa tcaacatatg gcttaccatc gggacttatt
     2821 ttgcctatct gccagagagg cccttctggt gaactcatca atttctgcaa gaatactttt
     2881 ttctccttat gtgccttaac tgccaattta agagctgaat caatctcttc aaaaaatctg
     2941 tcgaacttct ccccttcact gtaagacttt cctctctcta tacttttgag agtacggtag
     3001 gcacattgtg gaagattaat agtaacattt tgaataccgg aaaatcttaa tttttcggga
     3061 tatttaatca tatccatatc ggttatctca ttctttaacc ggcagcaagc tgcgagagat
     3121 acaccatccc gatcaaaaat aaaatatggt gtaccatttt ccgaagcaat ttgacaggca
     3181 tatttaagta attcaatctc ttcagggtcc tgaaaagagt taccatcaat atgtaagtcc
     3241 attttgggga aagcaaacac ctttccatta gaatcgccag cttcccatac ctccatcagt
     3301 gctttcaaaa actgctgcga ttcgctctta tattcttgat aggtctttcc ggtatatttt
     3361 ccacccggtc caatggccgg gacctctttt agatgttccg gaa
//
LOCUS       DAOQWT010000118         3387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_29377_length_3387_cov_3.128451, whole genome shotgun sequence.
ACCESSION   DAOQWT010000118 DAOQWT010000000
VERSION     DAOQWT010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29377_length_3387_cov_3.128451"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /gene="meaB"
                     /locus_tag="PKJ95_03150"
     CDS             complement(<1..871)
                     /gene="meaB"
                     /locus_tag="PKJ95_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNR65275.1"
                     /translation="MDLGTRVRQGDTRAVAKLITLVENDFQHAQKALKDLHQYCGKAI
                     VIGITGPPGSGKSTITDRLTQHIRKIGKKVGIIAIDPSSPFTGGAILGDRIRMQDLAT
                     DQEVFIRSIGTRGHLGGLSRSTQAIVKIMDAMGKDIIFIETVGVGQSEIEVIKVSDII
                     LLVTIPGMGDDIQALKAGIMEIGDIFVVNKADRDGADKLITEIEMMIHLNGEGKKNPP
                     IIKTVGIENIGIAQLWKEIERLIVELEENGQIYQRRRNRVRKEIFDLFQENWKDMFFQ
                     LIGEANLEREVEKV"
     gene            complement(888..1295)
                     /locus_tag="PKJ95_03155"
     CDS             complement(888..1295)
                     /locus_tag="PKJ95_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005386008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNR65276.1"
                     /translation="MKNARIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQT
                     PEQIVETAIQEDVNVIGLSILSGAHMYLFPKIMELLAEKEATDILVIAGGIIPNEDIA
                     ELKKVGIKEIFTPGTDTNKIVKFIKENIEITKT"
     gene            complement(1314..2990)
                     /locus_tag="PKJ95_03160"
     CDS             complement(1314..2990)
                     /locus_tag="PKJ95_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNR65277.1"
                     /translation="MNYQGKKRIEEVKKQWESNLLNKVLKKFSERKNTFVTGSNKEIN
                     RLYDPLDLESFDYVDDLGYPGEYPYTRGVQPTTYRGKLWTMRQYAGFGDAEESNKRYQ
                     YLLQNGQTGLSIAFDLPTQIGYDSTHPMAHGEVGKVGVAIDTLEDMEILFSGIPLDQV
                     STSMTINAPAAVLLAMYIAIAEKQGISKTELNGTIQNDILKEYVARGTYIFPPLPSMR
                     LITDIFDYCSKELPKWNTISISGYHIREAGSTAIQEIAFTLANGIAYVSAATKKGLDI
                     DIFAPRLSFFFNAHNDLFEEVAKFRAARRLWAKIMKERFRAKDPKSMMLRFHTQTAGC
                     TLTAQQPDNNVVRVAIQALAAVLGGTQSLHTNSRDEALSLPSQKSVRIALRTQQIIAY
                     ESGVAETVDPMAGSYYLEKLTTEIEQGAMDYINQIDKLGGAPKAIEKGFIQREIQNSA
                     YKYQKDIENKERIIVGVNQFQSEEEPMKDLLKVNPEIEKRQAEKLNSIKKRRNEAEVQ
                     ENLSLLKKIASSEQNVMPAILSCVRSYATLGEVCDQLREVFGEYKDSIKI"
BASE COUNT          943 a          737 c          473 g         1234 t
ORIGIN      
        1 atactttttc tacttccctt tccaggtttg cttcccctat taattgaaaa aacatatctt
       61 tccaattttc ctggaagaga tcgaaaattt cttttctaac ccgatttctt cttctctggt
      121 atatctgccc attctcctct aactcaacga tcaacctttc aatctctttc cataactgtg
      181 ctatacctat attctcaatg ccaactgttt taattattgg aggatttttt ttgccctcac
      241 catttaaatg tatcatcatc tctatttccg taatcagttt atcagcaccg tccctatccg
      301 ctttattgac tacaaagata tctcctattt ccataatgcc agcttttagt gcctgtatat
      361 catcccccat acctggaatg gtcaccagga gaataatatc cgacacttta attacttcta
      421 tttccgattg tcctacacca actgtttcta taaaaataat atctttcccc atagcgtcca
      481 ttatcttaac aatagcttga gtagaacgcg acaaacctcc cagatgacct ctggttccta
      541 tacttcggat aaatacttcc tggtcagttg ccagatcctg cattcttatg cgatcaccga
      601 gaatagcacc acctgtaaaa ggactgctgg gatctatggc aatgatacca actttttttc
      661 caatttttct gatatgctgg gtcaggcgat cagtaatggt gcttttacct gaaccgggag
      721 gcccggtaat tccaataaca atcgctttgc cacaatattg atgcaagtct tttaaggcct
      781 tttgagcatg ttgaaaatca ttctctacca aagtaataag ctttgctaca gccctggtat
      841 caccttgccg aactctggta cccaaatcca tttatttata cccctattta agttttggta
      901 atctcaatat tctcttttat aaattttact atcttattag tgtctgtccc tggagtaaat
      961 atctctttaa tacccacttt ttttaactcg gcaatatctt cattaggaat aattcctccc
     1021 gctattacca gaatatcggt agcctctttt tctgccaata actccattat ttttggaaat
     1081 agatacatat gcgcacctga gagaatactt aatccaataa cattaacatc ttcctgaatt
     1141 gctgtttcta caatctgttc aggtgtttgt cttaatcctg tatagataac ctccattccg
     1201 gcatctcgta atgcccgagc aataaccttt gctccacggt catgtccatc taaacctggt
     1261 ttggcaacta aaactcggat tctggcattt ttcatagtac accttccttg tctctatatc
     1321 tttatagaat ctttatattc accaaatact tctctgagtt gatcacaaac ctcgcccagg
     1381 gtagcataac ttctgacaca actaagaata gcaggcataa cattctgttc agaactggca
     1441 atcttcttta acaggctgag gttttcctga acttctgctt catttcttct ctttttaata
     1501 ctatttagtt tctctgcctg tctcttctct atttccgggt ttaccttcag caaatctttc
     1561 attggttctt cttctgattg aaattgattg acaccaacta ttattctttc tttattttca
     1621 atatctttct ggtatttata agcactgttc tgtatttccc tctgaataaa tcctttttca
     1681 atagctttag gtgctccccc caatttatca atctgattta tatagtccat tgctccttgt
     1741 tcaatttcag tggttaattt ttccaggtag taagaacccg ccatagggtc tacagtttca
     1801 gcaactccac tttcataggc aataatctgt tgtgttctta gggctattct tacggacttc
     1861 tgtgatggta aagaaagggc ttcatctctg gagtttgtgt gtaaagattg cgttcctcct
     1921 aaaactgcag ctaatgcttg aatcgccact ctaaccacat tattgtcagg ttgctgtgca
     1981 gtaagagtgc atccagcagt ttgagtatga aaacggagca tcattgactt tggatctttt
     2041 gccctaaagc gttctttcat aatttttgcc catagtctcc gtgccgctct aaatttagca
     2101 acttcttcaa atagatcatt atgtgcattg aagaaaaaag ataatctggg ggcaaaaata
     2161 tcaatatcaa gacctttttt agttgccgcg gaaacatacg caatcccatt agctaaagta
     2221 aaagctatct cttgaatagc agttgatcct gcttctctga tatggtaacc actaatactt
     2281 attgtattcc attttggcaa ttcctttgaa cagtaatcga aaatatcggt tattagtcgc
     2341 atggagggta agggtgggaa tatataggta cctcttgcca catattcttt taaaatatca
     2401 ttttgaatgg ttccatttag ttccgttttt gaaattccct gtttttcagc aatggcaata
     2461 tacattgcca acaaaacagc agccggagca ttgatagtca tagaagtgct tacctgatcc
     2521 agagggatac cactaaacaa tatctccata tcttctaaag tatcaattgc tactccaact
     2581 ttacctactt caccatgtgc cataggatga gtagagtcat aacctatttg agttggcaag
     2641 tcaaaagcga tacttaagcc agtctgacca ttttgcaaca gatactggta tcttttgttt
     2701 gattcctcag catctccaaa accggcatac tgtctcatag tccacaattt tccccgatac
     2761 gtagtcggct gtactcctct ggtatatggg tactccccag gatagcccag atcgtctaca
     2821 taatcaaaac tttctaaatc taatgggtca tacaatctat ttatttcctt atttgaacca
     2881 gtaacaaatg tattttttct ttcagaaaat ttttttaaaa ctttatttaa taaattactt
     2941 tcccactgtt ttttaacttc ttcaattcgc tttttccctt ggtaattcaa ctgctacacc
     3001 tccttttttt gttttgttaa aacttaaata aatttctggc tattttttca taaattattt
     3061 ttaaatattt tcttattatt cattttgatt tcagacaaaa accataaact caataatttc
     3121 atacttctaa ctaacatata tattattttc ataccacaaa caaagaattt atttataaca
     3181 aaatgaattt ttataaaata ctaaaaagca tttataatta ttaactaaaa tgaaaagaat
     3241 ttattctttt ccattattag actatctatc agcacatcaa tgtttttaat atttattccg
     3301 gtaatgtagt ccaattcttt tttcaattgt tcttgtattg ccctgaccat ttctggtata
     3361 tttacaccat attttaaagt gagattg
//
LOCUS       DAOQWT010000119         3366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_29632_length_3366_cov_3.033525, whole genome shotgun sequence.
ACCESSION   DAOQWT010000119 DAOQWT010000000
VERSION     DAOQWT010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29632_length_3366_cov_3.033525"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1417)
                     /locus_tag="PKJ95_03165"
     CDS             complement(101..1417)
                     /locus_tag="PKJ95_03165"
                     /inference="COORDINATES: protein motif:HMM:NF017347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HNR65278.1"
                     /translation="MDEKNKNLLPWGVILVISAAIFSGHFGVGDLIFPPILGRESGIC
                     WLIAALGYGVINSLGVFIAYLAVSIQDKSLFGLASSVLGKNFGAVFTTLSMLIIGPVF
                     ILPRVSSATHEMAVAQFFPNIPIWVTLLIFFALNYYFAYNRSKVIDNLGKFLAPILIV
                     FMVILVIKGIASPLASPVSPGSPTAFSEGILNGYNTMNALGASLFGLWLINEFNLRGV
                     KTKESRKYNLIIIGSITAIMLFLTSTVLTYLGASSGAKFPDADVGILTVEFARGLLGY
                     FGIIVFAIILTFANITTSIGLTSTAGDTFQQMTNGKLKYTTTVALSSVIGFLIGLIGL
                     SKVVGYTVPWLMLIYPALVVILVMNIFSNFERVKLATQIGTIVAILFSIGDFLPGIGF
                     ANNFFTRMNSSLPLGNQGLAWLVPTLIAVVAGLIVANYSKPKAEGV"
     gene            complement(1765..2541)
                     /locus_tag="PKJ95_03170"
     CDS             complement(1765..2541)
                     /locus_tag="PKJ95_03170"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNR65279.1"
                     /translation="MIENRMKRKIEKGIPVVGTFFGLNSTLAVECLGLAGLDFLVIDC
                     EHGPADVESTINFVLAAEFRGITPLVRVKNQNRDSILKMLDIGAKGLVIPCLETIDEV
                     RNIVKYGKYYPIGQRGVAMSRSGGFGFEDFSSGGLEKYFEICNKETLLIPQCETIGFL
                     DNIEVICAIDGIDGIFIGPFDLSVALKKPGKFDDKEFLSALRRVKRVAQDAGKFVFIH
                     TSTTQEAREHLKNGFDGVTLNQDVVMLTRAYQDEVNEIRS"
     gene            3029..>3366
                     /locus_tag="PKJ95_03175"
     CDS             3029..>3366
                     /locus_tag="PKJ95_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNR65280.1"
                     /translation="MKILLCTDGSEPSQKALEKIVMMAEKCKIEEIAIIHVYDKSQYT
                     SLPFFPDGGISITEEEIESLEKIKEKERIESKRLLAKASQIFKGKNIKVRTIFREGHP
                     SHVIVEVANK"
BASE COUNT         1165 a          654 c          499 g         1048 t
ORIGIN      
        1 ggactttcat ataatacaga atcctcagct aataccgtca aactcatcct gtctaaatat
       61 tcaaattcca tctgattatt actcctttct taaatcttgt ttacactcct tctgcttttg
      121 gtttagagta attagctaca ataagtcccg caaccaccgc tatcaaagtg ggtactaacc
      181 aggccaaccc ttgattcccc aggggcaggc ttgaattcat tctggtaaag aaattattag
      241 caaagcctat accgggcagg aaatctccta tactaaacag aatagcaaca atggttccaa
      301 tctgtgtggc taattttacc cgttcaaaat tagaaaatat gttcataacc aatattacta
      361 ccagagccgg atatatgagc attaaccagg gcacggtata acctaccact ttcgataagc
      421 ctattaaacc aattaaaaaa ccgattacac tgctcaaagc aactgtagtg gtatatttta
      481 gtttcccgtt ggtcatctgc tggaaagtat ctcctgcggt tgaagtaaga ccaatagagg
      541 tggttatatt ggcaaaagta agaataatag caaagacaat aataccaaaa tatcccaaca
      601 atcctctggc aaattccaca gttagaatac caacatctgc gtcagggaat tttgctcccg
      661 aagaggcacc taaataagta agaacagtag aggttaaaaa cagcattatt gcggtaatag
      721 aaccaattat aattaaatta tatttgcgtg attccttagt tttaactcct ctcaaattaa
      781 attcattgat aagccagaga ccaaataagg aagctcctaa agcattcatg gtattatatc
      841 catttaagat accttctgag aatgctgtgg gagaaccggg agaaacagga ctagccagcg
      901 gggaagctat tccttttatt actaatatga ccataaatac tatcaggatg ggggcaagaa
      961 atttccccag attatcaatg accttagagc gattataggc aaaataataa tttagggcaa
     1021 aaaagatgag taaggttacc catataggta tattggggaa aaattgggca acggccatct
     1081 catgagtagc agagctgact ctgggtaaaa taaagacagg acctattatt aacatagata
     1141 gggtagtgaa aactgcgcca aaattctttc ccaatacact ggaggccaga ccgaagagag
     1201 atttatcctg tatagagact gctaaatagg caatgaaaac tcctaaacta ttaataacac
     1261 catatcctaa tgcggcaata agccagcaaa ttccagattc tcttcccaat atgggaggga
     1321 aaatcaggtc acctacacca aaatgacctg agaaaatagc tgctgatatt accagtatta
     1381 ccccccaggg gagtaaattt ttgttttttt catccatatt attcaactcc ttctttatcc
     1441 ttcctcaatt ttaaaatagt gaagtagaaa aagtacaata actcgtgcag ttaagtgatt
     1501 ttgtctgttt tttaccatca atcacctcct caaaaaactt cctttgagta taaacttaag
     1561 aaaaatagtt acaaaagtaa aagatatagg atttttcatt aaactgacta tcataattca
     1621 aatttttaat attctcattc agtcaaacaa aataacaatg ataatcattt tctataaaag
     1681 atatataaaa aaatatatct tgtcaaatta tttctattgc tattctcagc ttatcacatc
     1741 acggtaatct caaaagatat tactttaaga ccttatttcg ttaacttcat cttgataagc
     1801 tctggttaac attaccacat cctgatttaa ggtaacacca tcaaaaccat ttttaagatg
     1861 ttctcttgcc tcttgagtag tgctagtatg gataaagaca aactttccag catcctgagc
     1921 aaccctttta acccttctta gtgctgataa aaactccttg tcatcaaatt ttcctggctt
     1981 ttttagggct actgataaat caaagggacc aatgaagatt ccatctattc catcaatagc
     2041 acatatcact tcaatattat ccaaaaaacc aatagtttca cactgaggaa ttaacaaagt
     2101 ttctttatta caaatttcaa aatatttttc taagccacca ctggaaaaat cttcaaaacc
     2161 aaatcctccg cttcgagaca ttgctacgcc ccgctgccct ataggatagt acttgccata
     2221 tttcacaata tttcttacct catctatcgt ttctaaacag ggaataacca gtcctttggc
     2281 accaatatca agcatcttca aaatagaatc acgattctgg tttttaaccc tcaccagtgg
     2341 cgtgatacca cgaaactccg ccgccagtac aaaatttata gtgctttcca catcggcagg
     2401 cccatgttcg caatctatta ccagaaaatc caagcctgcc agtcctaaac attccaccgc
     2461 caaggtactg ttcaatccaa aaaatgtccc tactaccggt atcccctttt ctatttttct
     2521 tttcatcctg ttttctatca tatggaaata tctctcctat tgttaatggt taaatactga
     2581 aaaatgattt cccacctatt atcattttat ataatattat atagcttttc tttacaattt
     2641 tcccctgtct aaatatcgtt ccatttaata ttttttacat taattttcac tttctctttt
     2701 gtaatattaa tttttttatc tacggatata taattattcc aaaagctatt aaacttgtca
     2761 ctataatttt atatttttct gcccaatacc aattattata atcgtgtctt ttaattcggt
     2821 ttatttgcac taatctaatt tttgagtccg aattttcttt atgtagagaa aataaaataa
     2881 taaaaaacaa gagaaaagat gcttaatttt tgtcaataaa taatcttacc atttatcata
     2941 tctattacaa aagttgtatc aggcaatgtt cagggtttaa cattttatga tatactttat
     3001 attaggttat aaaatatagg aggtttgtat gaaaattcta ttatgtactg atggttcaga
     3061 acccagtcaa aaagcattag aaaaaattgt catgatggca gaaaaatgta aaatagaaga
     3121 gatagctatc attcacgttt atgataagag ccaatacact tcccttcctt tttttccgga
     3181 tggtggtatc agcatcacag aagaagagat agaaagcctg gaaaagataa aagaaaaaga
     3241 aagaatagaa agcaaaaggt tattagcaaa agcttcccag atttttaagg gtaaaaatat
     3301 aaaagtcaga actatattca gggaaggcca cccctctcat gttattgttg aggtagcaaa
     3361 taaaga
//
LOCUS       DAOQWT010000120         3357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_29749_length_3357_cov_3.470927, whole genome shotgun sequence.
ACCESSION   DAOQWT010000120 DAOQWT010000000
VERSION     DAOQWT010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29749_length_3357_cov_3.470927"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PKJ95_03180"
     CDS             <1..292
                     /locus_tag="PKJ95_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNR65281.1"
                     /translation="TFNIIKGAMERLDRPDIALLHFQQLYPISEESIVFLDRAEQVIS
                     IENNYSGQFSRLLEAETGISVGTKILKYNGLPFYADELAREIDDILKEVAQK"
     gene            289..1152
                     /locus_tag="PKJ95_03185"
     CDS             289..1152
                     /locus_tag="PKJ95_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNR65282.1"
                     /translation="MNKTISFEQQIVKPAWCPGCGNFLILKALQQVFTELSLLPQEVV
                     IVSGIGQAAKTPQYLNVNMFNGLHGRALPPATAIKACNSKLTVIAEGGDGDMYGEGGN
                     HFIHTIRRNPDVTHLVHNNMVYGLTKGQASPTSDIGYKTPVQVDGVISDPINPIAMAI
                     SLGASFVARAYCGDIEHTIEIIKKAIQHRGYALVDIFQPCVTFNKVNTFQWFKDNTYI
                     LQDHDVHDINKAFIRATERDRLPVGIFYQNEQKETFEQRLKVLEEGKGDFLFRAMNKK
                     GLEELLKVREY"
     gene            1163..2707
                     /locus_tag="PKJ95_03190"
     CDS             1163..2707
                     /locus_tag="PKJ95_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNR65283.1"
                     /translation="MIIKDIERKFILIEIAFSKYGKIRLFSILIIFLIIFFNTTSFVL
                     AEALREAAFLQDYVVDKFDSAIFQLYLKSMEELDNQEIEFIDLLSEQSKDRQMLYGKL
                     VYQEGFSSNIFFQLKKEVEDRDRQLFPEEEDFIPQMLPFPTQPTMKWWERLYAHVVSM
                     RKVLTITEYNRFAMCGVDPARSERVVKRIKNYYDWNEKWFKEGQDVEYLAEKAAIEEN
                     IFLARRLFHEAAGCYHVGNFINFYDIKLKLESQEAARRCYVKAIALYEEKDRPIRIEI
                     PFREAEIPGYLMLNNKPNQPLIIFVNVLNNIKEVENHYFALDFLEAGFNVFSFDGPGQ
                     GEMHQKMRLIPDYEQAIITIIDWFENNNTFNIDMERIGVVGLSFGGLSSVIAAAQDPR
                     IDCVISNGGYAYIPPLSFIKKLHISTRLAVSYMSGINSMKVFYQKFGHLDIKNFPPLE
                     RPMLIIQGGKDDVVPAEHAFYFMDWAVGKDKELLYIEDSNHCCQDRFDIVMPYTIDWF
                     KKYLYE"
     gene            2700..>3357
                     /locus_tag="PKJ95_03195"
     CDS             2700..>3357
                     /locus_tag="PKJ95_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNR65284.1"
                     /translation="MSKGNKRLPNKIISRIVLGILRWYLSTIFFLKLERNDTLKMTPP
                     YLLIGNHANFWDGFLVNLFIKEPICFLVSDEYFRTPLFKMLLQIEGSIPKKKFMTDLA
                     AVKEALRAKEAGRIIGVFPEGMRNWDGSVGELIFATAKFIKMLNIPVVRVLSKGSYLS
                     FPRWAHYKRKGKIIFDYELIMMAEQIKEMSIDEIFRKINDSLSYNEYDYQRKTMNTYQ
                     G"
BASE COUNT         1085 a          519 c          724 g         1029 t
ORIGIN      
        1 aacattcaat attattaaag gagcaatgga gaggctggat aggccggata ttgctttact
       61 ccattttcaa cagctttatc ctatttctga ggaaagtatt gtttttttgg acagggcaga
      121 gcaggttatt tctattgaaa ataactatag tggacaattc agccgtctgt tagaggcaga
      181 aacaggaatc agtgtaggaa caaagatact caaatacaat ggtctgccct tttatgctga
      241 tgagcttgcc cgggaaatag atgatattct aaaggaggta gctcaaaaat gaataagact
      301 ataagtttcg aacaacagat tgttaagccg gcctggtgtc ctggctgtgg caattttctg
      361 atattgaaag cattgcagca ggtttttaca gaactatctt tgttacctca ggaagtggtc
      421 atcgtctccg gtatcggtca ggcagccaaa actccacaat atctaaatgt aaatatgttc
      481 aatggattac acggcagagc tttgccacct gctaccgcaa ttaaagcatg taattcaaaa
      541 ttgaccgtta ttgcggaagg tggagatggt gatatgtacg gagagggtgg aaaccacttt
      601 atacatacca tcaggcgaaa tccagacgta acccatttag tccataataa tatggtctac
      661 gggcttacca aaggacaggc atcacccacc agcgatattg gatataaaac accggtgcag
      721 gtggatgggg tcatatctga ccctattaat ccaatagcta tggctatctc tctgggagcc
      781 tcttttgtag caagggctta ttgcggtgac atagagcaca ctattgaaat tattaaaaag
      841 gcaatacagc atcgtgggta tgctttggta gatattttcc agccctgtgt taccttcaat
      901 aaagtaaata cctttcaatg gtttaaggac aatacttata ttctccagga tcacgatgtt
      961 cacgatataa ataaggcttt tattcgggca actgaaagag acaggctacc ggtagggatt
     1021 ttttatcaga atgaacagaa agaaaccttt gaacagaggt taaaggtact agaagagggc
     1081 aaaggagact ttttattcag agctatgaat aaaaagggtt tagaggagct gttaaaagtt
     1141 agagaatatt gaaataaaat aaatgattat aaaagatata gaaaggaagt ttatattgat
     1201 tgagattgcc tttagtaaat atggaaaaat aagactattc tcaatactga ttatcttttt
     1261 gatcattttt ttcaacacta caagttttgt tcttgctgaa gccctccgag aagctgcttt
     1321 tttgcaggat tatgtggtag acaagtttga ttcagcaata tttcagctat atttaaaatc
     1381 gatggaggaa ttggataatc aggaaataga atttattgat ttactctcag aacaatcaaa
     1441 agataggcag atgttatatg ggaaactggt ttatcaggag ggtttcagtt caaatatatt
     1501 ttttcagcta aaaaaagaag tagaggatag ggataggcag ctatttcctg aagaagaaga
     1561 ttttatccca cagatgttac catttccaac ccagccaact atgaaatggt gggaaaggct
     1621 ttatgctcat gtggtttcaa tgagaaaagt ccttactatc actgagtata atcgttttgc
     1681 catgtgtgga gttgaccccg cacgtagtga aagagtggta aaaagaataa agaattatta
     1741 tgattggaac gagaaatggt ttaaagaagg acaggatgtt gagtatttag cagaaaaagc
     1801 tgccatagag gagaatattt ttttggctcg cagactattt catgaggctg ctggttgtta
     1861 tcatgtaggg aattttatta atttttatga tataaaatta aaattagaat cgcaagaggc
     1921 agcccgaagg tgttatgtga aggcaattgc attatacgaa gagaaggaca gacctattag
     1981 aattgaaata ccttttcgag aagcagaaat ccctggttat cttatgttaa ataataaacc
     2041 aaatcaaccc ttaattatct ttgttaacgt gttgaataat atcaaagagg tagaaaatca
     2101 ctactttgca ctggattttt tagaagcagg atttaatgta ttcagttttg atgggccagg
     2161 acagggagaa atgcatcaga agatgcgttt aattcctgat tatgagcagg ctattattac
     2221 cattatcgac tggtttgaaa ataataatac ctttaatatt gatatggaaa ggattggagt
     2281 agtgggattg agttttggtg gattatcttc agtgattgcc gcagcccagg atcccaggat
     2341 tgattgtgtt atcagtaacg gcgggtatgc ctatatccct ccactttcct ttattaaaaa
     2401 attacacatt agcacacggc tagctgttag ctacatgagt ggtattaata gtatgaaagt
     2461 gttttaccaa aaatttggcc atcttgatat taagaatttt ccacccttag agcgtcctat
     2521 gttaattatc cagggtggga aggatgatgt ggtacctgca gagcatgcct tttactttat
     2581 ggattgggca gttggcaaag ataaagaatt gctctatatt gaagattcta accattgctg
     2641 tcaggaccgt tttgatattg ttatgcccta tactatcgac tggttcaaga aatacttata
     2701 tgagtaaggg aaacaaaaga ttacctaata aaataatttc cagaatagtt ctcggtattc
     2761 ttagatggta cttgagtact atattttttt taaagctgga aagaaacgat acactaaaga
     2821 tgaccccacc ctatctgttg ataggcaatc atgccaattt ttgggatggt tttttagtta
     2881 atctttttat caaagaacca atctgctttc tggtttcaga tgaatatttc cggacccccc
     2941 tttttaaaat gttattgcaa attgagggct ctattcctaa aaagaagttt atgacagatc
     3001 tggcagctgt gaaagaggct ttgagagcca aagaggctgg gcgcataatc ggtgtcttcc
     3061 cggaaggtat gcgtaattgg gatggttctg taggagagct aatatttgct actgctaaat
     3121 ttattaaaat gctcaatata ccggtagtaa gagtgttatc taagggctca tatctctcct
     3181 ttccccgttg ggcacattat aagaggaaag gaaaaatcat ctttgattat gaattgataa
     3241 tgatggctga acagattaaa gaaatgtcga ttgatgaaat atttcgtaaa attaatgata
     3301 gcctttctta caacgaatat gattatcaga gaaagactat gaatacttac cagggta
//
LOCUS       DAOQWT010000121         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_29777_length_3355_cov_3.551818, whole genome shotgun sequence.
ACCESSION   DAOQWT010000121 DAOQWT010000000
VERSION     DAOQWT010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29777_length_3355_cov_3.551818"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..396
                     /locus_tag="PKJ95_03200"
     CDS             16..396
                     /locus_tag="PKJ95_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNR65285.1"
                     /translation="MAQQYEIYKCEICRNIVEVLHGGKGELVCCGEPMILMEEQTAEQ
                     VYEKHVPVVEKTENGIKVKVGSVPHPMEEKHYIEWIQVITDKGACRKFLHPGDAPEAE
                     FVDIEGLQRVREYCNIHHLWKYEE"
     gene            404..1300
                     /locus_tag="PKJ95_03205"
     CDS             404..1300
                     /locus_tag="PKJ95_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR65286.1"
                     /translation="MKKAELWFCLFLIFIFFNAAISFFNQICLAQSKWNQVEVYSDIN
                     YYPENISEETSKNAHLLDIYKPEYCQSCPVIIYIHGGYWVMGDKGGLSYKVKAFTSQN
                     YIYISINYSLSPDYQFPAHAYDVARAFSWVKNNIKKYGGNPEQIYLLGHSAGGQLAAL
                     MALDEHYLAEFGLAPSDIAGIIGLDSAGYHLPSLFVAEPENQYLISWAFGDEPEDLET
                     ASPINYIKKGLNIPPFLLLVAGGRNVSVEVNNSFYQKLIDNGYQADILQFSEKDHVSI
                     DYELGKDKDPVFPAVLNWLKEH"
     gene            complement(1430..1861)
                     /gene="pgsC"
                     /locus_tag="PKJ95_03210"
     CDS             complement(1430..1861)
                     /gene="pgsC"
                     /locus_tag="PKJ95_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HNR65287.1"
                     /translation="MIFQAIGIGIAIGFLFYELVGLSPGGIVTPGYIALFINQPWRII
                     ITIAIAILTYYIVIFLSNYLILYGKRRFLSMILISFLVKWLVESFLFQLPVATIEIQS
                     IGYIIPGLLANEMQRQGILPTLLATIIVAILVRLSLYLFLF"
     gene            complement(1858..3039)
                     /gene="pgsB"
                     /locus_tag="PKJ95_03215"
     CDS             complement(1858..3039)
                     /gene="pgsB"
                     /locus_tag="PKJ95_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HNR65288.1"
                     /translation="MFLLFFLGIVILIMGILENEFHRKRVNTIPIRIHVNGTRGKSTT
                     VRLIAAILREAGYRTLAKTTGTSPKIILENGDEELIRRKGPSNIIEQKYFIKKAFFKR
                     CNAIVVECMAVHPETQWVSEHRLIYSTIGVITNVREDHQDVYGPDLKDAANSLMATIP
                     KNGVFITADKKFFPLFQSLAEKLNSKSILVDPKEISFPEKEKSENILFKENVAIALQI
                     GQLLNIEENICWKGILKARPDPGALTIYKLKVEGKLLWFVNAFAANDRESILLIWDKI
                     NQLLPEKVVNSPKIAILNNRNDRITRIVQFASILSKEITVDHIFLAGQNTTLSYNQLR
                     KNGYPSQQITAVNEKVDIDHTMEQISHFIEEEGILYGLGNTRGFGLRLMDYLQEKGEK
                     L"
BASE COUNT         1093 a          563 c          602 g         1097 t
ORIGIN      
        1 aaggagaaat agaatatggc tcaacaatat gaaatataca aatgtgaaat ctgtagaaac
       61 attgtagagg tcttacatgg cggaaaaggt gaactggtct gttgtggaga accaatgata
      121 ttaatggaag agcagactgc cgaacaggtt tatgagaaac atgtcccggt agttgaaaag
      181 acagaaaatg gtattaaagt taaagttggt tcagtaccac atcccatgga agaaaagcat
      241 tatattgaat ggatacaggt aattacagat aaaggagcct gcaggaaatt tttacatccc
      301 ggagatgcac ctgaggctga atttgtagac atagagggct tgcagagggt cagagaatat
      361 tgtaatatcc atcatctatg gaaatatgaa gagtaaaaaa tacttgaaaa aggcagagct
      421 ctggttctgc ctttttttaa tatttatatt tttcaacgct gcaatttctt tttttaacca
      481 aatttgtctg gctcaatcaa aatggaatca ggtagaagta tattctgata ttaattatta
      541 tccggaaaat atttcagaag agacttccaa aaatgcacac ctattggata tttataagcc
      601 cgaatactgt caatcctgcc cggtcatcat ctatattcat ggtggttact gggttatggg
      661 tgataaggga gggttgagct ataaggtaaa agcctttact tctcaaaact atatctacat
      721 tagcataaac tatagcctat cgccggatta tcaatttcca gctcatgctt atgatgttgc
      781 ccgtgccttt tcctgggtaa aaaataatat taaaaagtat ggcggtaatc cagaacaaat
      841 atatctttta ggccattctg ctggtggaca actggctgct ctgatggctt tagatgaaca
      901 ttatttagct gaatttggat tagctccatc agatattgcc ggaattatag gattggatag
      961 tgcgggatat catttgcctt cattatttgt tgcggaacca gaaaatcaat atctgatttc
     1021 ctgggctttt ggggatgaac ctgaggatct tgaaacagcc tcacctatta attacatcaa
     1081 gaagggacta aatatacctc catttttgct attggtagca ggtggacgta atgtctctgt
     1141 ggaggtaaat aattcttttt atcaaaaact gattgacaat ggttatcagg cagatatatt
     1201 acaattctca gaaaaagacc atgtttccat cgattatgag cttggaaaag ataaagaccc
     1261 ggttttccct gctgttttaa attggctaaa ggaacattag gaaaatcctc tgtatcttgt
     1321 attatgaata taacccataa tggactatct tattatatag agataattgc gaattaaaaa
     1381 atttcaatac gattcgatgt ggtttaaagt atttttgtcg gcttaataat tagaataaaa
     1441 aaagataaag ggaaagacga accagaatgg ctacaatgat ggtcgccagt aaggttggta
     1501 aaatcccctg cctttgcatc tcatttgcca acagtccggg tataatataa ccgatactct
     1561 gtatttcaat agtggcaaca ggtaattgaa ataagaaact ttctaccagc cattttacca
     1621 aaaagcttat caaaatcata cttaaaaatc gtctttttcc atatagtatc agataattag
     1681 ataagaagat tactatataa taagtcagta ttgcaatagc gatggttatg ataattctcc
     1741 agggctgatt gataaaaaga gcaatatagc caggggttac tattcctccc ggagataatc
     1801 ctaccaattc atagaataaa aaaccgatgg cgataccaat tccgatggct tgaaatatca
     1861 taatttttct cctttttctt gcagataatc cattaatctt aagccaaacc cacgggtatt
     1921 ccccaaacca tacaatattc cctcttcttc tatgaaatga gaaatctgtt ccatagtatg
     1981 gtctatatca actttttcat ttactgcagt aatctgctga gaggggtaac cgttttttct
     2041 gagttgatta taactaagcg tggtattttg ccccgccaga aagatatggt ctactgtaat
     2101 ttctttactt aaaatgctgg caaattgaac aattctggta atcctatcat tgcgattatt
     2161 aaggatggcg attttgggag agtttactac cttttcaggc agcaattgat ttattttatc
     2221 ccaaatcaac aaaattgatt cacggtcatt ggcggcaaag gcattaacaa accataacag
     2281 tttaccctct acctttaatt tataaatagt aagggctcct ggatccggtc tggcttttaa
     2341 tatccctttc cagcaaatat tttcctcaat attcaggagc tgtcctatct gtaaggcaat
     2401 agccacattt tctttaaaca aaatattttc tgatttttct ttttctggaa aactaatctc
     2461 tttgggatcg accagaatag atttagaatt taatttttct gccagacttt ggaacaaggg
     2521 gaagaatttt ttatcagcag ttataaaaac cccattttta ggtatagtgg ccattagtga
     2581 atttgctgca tcctttaaat caggcccata gacatcctga tggtcttctc tgacattggt
     2641 aatcacacca atagtggaat aaatcaatct atgttcacta acccactggg tttcagggtg
     2701 tacagccata cattcaacca cgatagcatt acatctttta aagaatgctt ttttaataaa
     2761 atatttttgc tcaatgatgt ttgaaggtcc ttttcttctt attagttctt catcaccatt
     2821 ttccaggata attttgggac tggttccggt tgtttttgcc agggtacggt aaccagcttc
     2881 tcttaaaata gctgcaatca atctcaccgt agtagatttt ccacgggtac cattgacatg
     2941 gattctaatg ggaatggtat taactctttt cctatggaat tcgttttcta aaattcccat
     3001 tattagaatt acaatgccaa gaaagaataa tagaaacata aataaatctt tcttttttac
     3061 ggaatatttt gtcctaatgt tataggaaag ttgtttgaat gtaaaaatag aacaattaca
     3121 gtcaactata ttctactatt ttcagtaaaa ataaacaaaa aactcgcttt gcttggaagc
     3181 aaagcgagtt tgaaaggagg tataaaaatg aaaagaatat ttaatggttt tatttcttta
     3241 ttccaaaacc agaaatatta tagcactgtt tttattcttg tcaagggcaa attaaaaaat
     3301 tttgtgatta atttgtgata atgtcacctt attaattatc ttgataaaat gtcac
//
LOCUS       DAOQWT010000122         3332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_30088_length_3332_cov_3.469332, whole genome shotgun sequence.
ACCESSION   DAOQWT010000122 DAOQWT010000000
VERSION     DAOQWT010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30088_length_3332_cov_3.469332"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKJ95_03220"
     CDS             complement(<1..249)
                     /locus_tag="PKJ95_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65289.1"
                     /translation="MKGKNVLTITIFLLLIILSNSLLLAETGIKEASTYKSNGDLING
                     WNWLRDSALNDYAEWTFHNITPGTTDLVLDITALATDKV"
     gene            complement(404..1582)
                     /locus_tag="PKJ95_03225"
     CDS             complement(404..1582)
                     /locus_tag="PKJ95_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65290.1"
                     /translation="MLTNGLVKKHQKIVLLLVFIYLILFILFLFTLEIAAFPNISPET
                     LGSLPVNPILLDDDSLNELIYISSDFSILVFRRATFQLLNLNMGDALFLDYEITKDDY
                     FLGQIINIIRDIRDNSYQNGMIIHLVPWRENHPPVISGLIAQPSILEAGQQSRVTCHA
                     ADEDGDTLSYYWYSNKGLIRGIGASVIWTAPYQPGDCFISCEVSDNRGGKDNRAVRIQ
                     VVKSSPLLTYQERELIYQFGWSSNHTIRWPDGPVAVYDGTNFHRMQEVLDEWNKVVGD
                     NVLFYLSNDPQSPVKISYNYELSRKNLCYHVDTHWRNYQLYAAEIQVNPDSWQCGFPG
                     NLYAGYLHSFSGVAGFNVWQGKTIERRDWQNFNQISEIMRNMIKGLYKVPAGYDLNND
                     "
     gene            complement(1989..2963)
                     /locus_tag="PKJ95_03230"
     CDS             complement(1989..2963)
                     /locus_tag="PKJ95_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNR65291.1"
                     /translation="MRTFLGIDVGAVSTDLVLLDENNDVLNSVYLRTQGQPIKMLKEG
                     LSIIRKENQGISLEVVGVGTTGSGRVLAGVIAGADIVKNEITAHSVAALYLNPEVRTI
                     FEIGGQDSKIIILQNGVVVDFAMNTICAAGTGSFLDHQAYRLDIPIEEFGEYALRSEN
                     PVRIAGRCTVFAESDMVHKQQMGYKEEDIIAGLCEAMVRNYLNNVGKGKKILPTIFFQ
                     GGVAANSGIKKSFEKEIGYQVVVPEYYQVMGAIGAALLAREEIELNPRPSAFYGFELV
                     ERNYQTSGFECDGCPNHCEVVEIMVDGETIARWGDRCGKWSLGYQAIR"
     gene            complement(2960..>3332)
                     /locus_tag="PKJ95_03235"
     CDS             complement(2960..>3332)
                     /locus_tag="PKJ95_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HNR65292.1"
                     /translation="IEAWIGPIMRLKIFKDSFKEEQEIEEAAYPYLAHSVGGEGQTTV
                     GNVVLAHKEGLDGAIQVIPFTCMPEIVAETILKKVSEDLDFPTLTLIYDEQTGEEGLN
                     TRVEAFVDLLKKRRKGVRMSV"
BASE COUNT         1015 a          750 c          550 g         1017 t
ORIGIN      
        1 tactttatca gtagccagag cagtgatatc caaaaccaga tctgttgtgc caggagttat
       61 gttatgaaaa gtccactcgg cataatcatt caatgccgaa tccctaagcc agttccagcc
      121 attaattaaa tctccatttg atttgtaggt acttgcctct tttataccgg tttcagctaa
      181 taagagtgag tttgataaaa ttataagcag taaaaaaata gttatagtta aaacattttt
      241 tcctttcata attattacct cctaccattt tattttttta taatcaaatt taaacaacct
      301 accattatta ttattccctt ttttcataaa tatagacaac caggtaatat ttttcgctta
      361 atttctcagg caacaataat ttaaaaaaat ttattgacac ttattaatca ttgtttaaat
      421 cataacctgc gggaactttg taaagacctt ttatcatatt gcgcattatc tctgaaatct
      481 gattgaaatt ttgccagtct cgccgttcaa tggttttccc ctgccagaca ttaaagccgg
      541 ccactccgga aaaggaatgg agatagccgg catacaggtt tcctggaaaa ccgcattgcc
      601 aggaatctgg attaacctga atctcggcag catacaattg gtaatttctc caatgggtgt
      661 caacatgata acagaggttt tttcggctca actcatagtt gtaacttatc tttaccggac
      721 tttgagggtc attacttaaa taaaaaagaa cattatcacc taccacctta ttccactcat
      781 ccaacacttc ctgcatgcga tggaaatttg tgccatcata gactgctaca ggaccatcag
      841 gccatcgaat ggtgtgattg ctgctccagc caaattggta tatcaattct ctttcctgat
      901 aagttaataa aggagaactt ttcacaacct gaatccgaac tgccctatta tctttgcctc
      961 ccctgttgtc actaacttca caactgataa aacaatcacc gggttgatag ggagctgtcc
     1021 agattacact ggcaccgatt cctcttatca agcccttatt gctataccaa tagtaggaaa
     1081 gggtatctcc atcctcatca gcagcatgac aggtgaccct gctctgctgt cctgcttcta
     1141 agatagaggg ttgagcaatc aaaccggaaa tgaccggagg atggttttcc cgccagggaa
     1201 caaggtgaat aatcattcca ttttgataag aattatctct aatatcccta attatattga
     1261 taatttgacc taaaaaataa tcgtctttag ttatctcata atctaagaat aaagcatccc
     1321 ccatatttaa atttagcagt tggaaagttg ctcttctgaa gaccagaata ctgaaatcag
     1381 acgagatgta gataagttca ttaagtgaat catcgtctaa caatattgga tttaccggca
     1441 gagagcccag agtctctgga gagatatttg ggaaggctgc tatttctaag gtaaataaga
     1501 aaaggataaa aaggataaga taaatgaata ctaataataa gacaatcttt tggtgctttt
     1561 taaccaaacc attggtaagc acagccagac ctcctatttt tttaaaaatc ttaactatgc
     1621 catataataa ccacacaata cactagaaaa taatattttt ttactattat ttattctatt
     1681 tctttaaaag aatggtactt agactctagc ttcctaactc tttaaagtca gaaaggcaat
     1741 gaatgatcaa ggaatcatca gtcaaagtcc aattatgata tagttcattt tttattttta
     1801 ggagaaatcc cagctgaata tttcatctgg cagattaatt ttcaaaaaga aatgtaatta
     1861 ttttatatta tactattttt tgaaaaaatt agtaataaca tttatagcag aagggataaa
     1921 tatagttatc agtcactggt agttgatatt tattagttag attagatttt aagtttatag
     1981 ctgacttcct atcgaattgc ttgatagcct aaagaccatt tgccacagcg gtcaccccag
     2041 cgggcaatcg tttcaccatc aaccatgatt tccaccactt cacaatggtt gggacaacca
     2101 tcacattcaa atccactggt ctggtaattc cgctccacca attcaaaacc atagaaggcc
     2161 gagggacgag gatttagctc tatctcttcc ctggccagaa gggctgcccc tatggcaccc
     2221 atcacctggt aatactcagg aactacaacc tgataaccaa tctctttttc aaaactcttt
     2281 ttaattcctg aattggcagc cacacctccc tgaaagaaaa tggtaggtaa aatcttcttg
     2341 ccctttccca cattgttcag gtaattgcgg accattgcct cacataaacc ggcaataata
     2401 tcttcttctt tgtagcccat ctgctgtttg tgaaccatat ctgattcggc aaatactgta
     2461 catctgcctg caatccttac cggattttcg gaacgcagag catactctcc aaactcttca
     2521 atgggaatgt ccagacgata tgcctgatga tccaggaaag aaccggtacc ggcagcacag
     2581 atagtattca tggcaaaatc gaccaccaca ccattctgaa gaataatgat tttggaatcc
     2641 tgcccgccta tctcaaagat ggtacgcact tccggattaa gatataaggc tgctaccgaa
     2701 tgagcggtaa tctcattctt cacgatatcc gcacctgcta ttaccccggc taatactctt
     2761 ccactaccgg tagtaccaac gccgaccacc tccagagata ttccctgatt ttcttttctg
     2821 ataatggaga gtccttcttt gagcatttta atgggctgcc cctgtgtcct cagataaaca
     2881 gagtttaaca catcgttatt ctcatccaat aagaccagat cggtactgac cgcccctaca
     2941 tcgattccta aaaatgttct catacagaca tccttacccc ctttctcctc tttttaagca
     3001 agtcaacaaa agcctctacc cgggtattaa gcccttcttc accggtttgc tcatcataaa
     3061 tcagggttaa agtaggaaaa tcaaggtctt cactaacctt cttcaggatt gtctcggcaa
     3121 caatctcggg catacaggtg aagggtataa cctgaatggc accatccagc ccttctttat
     3181 gggctaacac cacattgccc actgtagtct gtccttcacc gcctaccgaa tgtgccagat
     3241 aagggtaggc tgcctcctct atctcttgtt cttccttaaa actatcttta aaaattttta
     3301 atctcattat ggggccaatc caggcttcaa tg
//
LOCUS       DAOQWT010000123         3331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_30107_length_3331_cov_2.888584, whole genome shotgun sequence.
ACCESSION   DAOQWT010000123 DAOQWT010000000
VERSION     DAOQWT010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30107_length_3331_cov_2.888584"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..634)
                     /gene="greA"
                     /locus_tag="PKJ95_03240"
     CDS             complement(161..634)
                     /gene="greA"
                     /locus_tag="PKJ95_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012772085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNR65293.1"
                     /translation="MNERKFMLTEEGLEKLKKELYELKTVKRKEIAEKIGSAVAFGEI
                     AENAEYRAAKNEQAFIEGRILTLEKIIENCEIIDRSKNDGIYVSPGSEIVVEDLKKKE
                     KINFTLVDYIESDPGSGKISMTSPIGKAVLGKKAGDKVKIRVPAGNLHYKILEIK"
     gene            complement(892..2082)
                     /locus_tag="PKJ95_03245"
     CDS             complement(892..2082)
                     /locus_tag="PKJ95_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR65294.1"
                     /translation="MAISLEQRIEETNERAKRMKENDSFFYLKSIEGSSGAHTRIEGR
                     EMLMFASYNYLGLISHPKVKEAAKKAIDEYGTGAAGVRFLAGTTRAHEELEKKIAQFK
                     KTDDAVTYSSGYVTNLAALTTLCQRGDLLIMDKLVHASIIDGCILSGANHRSFLHNDM
                     DSLERILAQSDGYQNKLIVIDAVYSMDGDIANLPEISRLAKKYKAKLMVDEAHSIGVI
                     GKTGHGIEEYFGLENSVDIYMGTLSKTIPAIGGYLAGDKDLISFLKLSSRPFIFSASL
                     PPVAVLTAKACFEVIENEPELVVKLQKNMKQFREGLQEMGYNTGNSETAIVPIMVGDE
                     EKTLQLCKKINEEGVFICPILFPAVPRGTNRLRAHVLASHTAEDIEKALDIFKQAGKS
                     LGII"
     gene            complement(2172..3080)
                     /locus_tag="PKJ95_03250"
     CDS             complement(2172..3080)
                     /locus_tag="PKJ95_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HNR65295.1"
                     /translation="MKKVLGVSLGSSTRDSKVKIEILGEEVEIERRGTDGDVSKMMQI
                     YREYDGKVDVFALGGTDLYLYSGPSGKRYTIKESARIVSVIKKTPIVDGTGIKYLLEK
                     DAIRYAKENTDIDFQGKKALILITVDRFGLAEGLIEAGCKMIFGDIIFCLNLPIPLYS
                     LEVLNIVARIAIPILANLPIKYLYPTGKKQEGRNPKYSRFFEGVDIVAGDYLAISRYM
                     PPRMEGKIVITNTVTMQNVQELKDSGVKYLITTTPQFEGRSFGTNIYQATLIAASGKK
                     PEELTGQDYLDLAKKSGFKPRIEQLN"
     gene            complement(3077..>3331)
                     /locus_tag="PKJ95_03255"
     CDS             complement(3077..>3331)
                     /locus_tag="PKJ95_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65296.1"
                     /translation="KVRDKGKLFNDTLDIITYLLDFDVIIFLGSDKRVDFAEKLFHIQ
                     VIGIEIGKSPITEDKYVDPKIIENVLKEVKIISLNEEMDK"
BASE COUNT          972 a          712 c          485 g         1162 t
ORIGIN      
        1 ttgaactact ggcaagtgat atcttagaaa acccctacta aaattttaca ctatcaagag
       61 tgcttattat aatttatttt ttggaataaa aaaagacaga gcttttaaat tatagaaatg
      121 ctctgccatt tataaattca ataagtattt atttaattat ttatttgatt tctaatattt
      181 tataatgcaa gttcccggca ggaactcgaa tctttacctt atcccccgct tttttcccca
      241 atactgcttt accaatggga gaagtcatag atatcttgcc ggaaccgggg tcagattcta
      301 tataatcaac taaggtgaaa ttaatctttt cttttttctt taaatcttct accactatct
      361 ccgagccagg agaaacatat ataccatcat ttttgcttct atcaattatt tcacaatttt
      421 cgataatttt ttccagagtt agaattcttc cttcaataaa agcctgttca tttttagccg
      481 cccggtattc agcattttcg gcaatttcac caaaggcgac agcactgccg attttttcag
      541 caatttcttt tcttttaacg gtctttaatt catataattc cttttttaat ttttccaggc
      601 cttcctcagt cagcataaac ttccgctcat tcatttttat gcaccctatc ctttaatata
      661 aaatataact aagattatgc aaagtattta atattaaatc caccttaatt gtttttatat
      721 tatatctatt tatgcctttc ttctgatgtg attatttaga caaattatag attacttctt
      781 tttccttgtc aaataaagtt acataacaag gctatgggca aaagaaaaat tccttttcta
      841 tttaactcaa tctgaccagg aacaaatcca taaaacctat atatttagga tttagataat
      901 tcccaaagat ttacctgcct gcttaaagat atcaagagct ttttctatat cttccgcagt
      961 atgactggca agtacatggg ctctcagtct attggtgcct ctgggaacgg ctggaaagag
     1021 aatgggacag ataaaaacac cttcctcatt gatcttttta cataattgta atgtcttttc
     1081 ttcatctccc accataattg gcactattgc cgtttcacta ttgccggtat tgtaccccat
     1141 ttcctgtaat ccttctctaa actgtttcat atttttttgt aatttcacta ctaactccgg
     1201 ttcattttct attacctcaa aacaggcttt ggcagtaaga acggctacag ggggtagact
     1261 ggctgaaaat atgaagggac gagatgatag tttaagaaaa ctgattaaat ctttatcccc
     1321 tgctaagtag ccacctatag caggaatagt cttgcttaaa gtccccatgt agatatccac
     1381 tgagttctcc aaaccaaagt attcctcaat tccatgtccg gttttgccaa taacgccaat
     1441 agaatgtgcc tcatctacca taagcttagc cttatatttc tttgccaaac gagaaatttc
     1501 cggtaaatta gctatatcac catccatgct atagacagca tctatcacaa tgagtttatt
     1561 ctggtaaccg tcagattgtg ctaatattct ctccaggctg tccatatcgt tatgtaaaaa
     1621 actacgatga ttggctcctg ataagataca accatcgatg atactggcat ggaccagctt
     1681 atccataatt aacagatcac cacgctggca aagagtggtc agggcagcta aattagtaac
     1741 atagccacta ctgtaggtta cagcatcatc agtcttttta aactgggcaa tctttttttc
     1801 cagctcttca tgtgctctgg tagttcctgc caaaaaccgt acacctgctg ctccagtccc
     1861 atattcatct atggcttttt ttgctgcctc ttttacttta ggatgtgata ttaaccctaa
     1921 ataattgtat gaggcaaaca taagcatttc acgtccttct attcgcgtat gtgctcccga
     1981 tgatccctct atactcttta aataaaagaa ggaatcattc tccttcattc tttttgctcg
     2041 ttcattggtt tcttcaattc tctgttctaa agatatagcc attatttttc cctctcaatt
     2101 taattaagat atattaaaat agtttactaa aaactatagc aattatattg aacaacctct
     2161 ttctatggcc attagtttaa ctgctcaatc ctgggcttaa accctgattt tttggccaaa
     2221 tcgagatagt cctgtccggt aagctcctca ggttttttac ctgatgcggc aattaaggta
     2281 gcttgataga tattggtacc gaaagacctc ccttcaaact ggggagtggt ggtaataaga
     2341 tattttaccc cgctatcttt taattcctgc acattctgca tagttaccgt attggtaata
     2401 acgattttcc cttccattct gggtggcata taacggctta tagcaagata atcaccggca
     2461 acaatatcaa ctccttcaaa gaaacggctg tatttcgggt tacgcccctc ctgtttcttt
     2521 ccagtggggt ataaatattt tatagggaga tttgccagga tcgggatggc gattcgggct
     2581 actatgttta atacctccag gctgtaaagc ggaatgggta aattgagaca gaagataata
     2641 tccccgaaga tcatcttgca gccagcctca atcaatcctt cagccagacc gaatctatca
     2701 acagtaatta atatcaaagc tttcttcccc tgaaagtcta tatcagtgtt ctctttagca
     2761 tatctgattg catctttttc cagtaaatat ttgattccag ttccatcaac aataggagtc
     2821 tttttgatta cacttacaat tctggctgat tctttgatag tatacctttt tccggaagga
     2881 cctgaataaa gatagaggtc agttcctccc agggcaaata catctacttt gccatcatat
     2941 tcccggtata tttgcatcat tttagaaaca tcaccatcag tacctcttct ttcaatctca
     3001 acctcttctc ccaaaatttc aatttttact ttgctatccc tggttgaaga acctaaacta
     3061 acccccagta cctttttcat ttatccatct cctcattcaa tgaaattatt tttacttctt
     3121 ttaagacatt ttcaataatc tttgggtcta catatttatc ctcagtgatt ggtgatttac
     3181 caatttcaat accaattacc tgaatatgaa ataacttttc tgcaaaatca actcttttat
     3241 cagagcccaa aaaaataatc acatcgaaat caagcaaata agtgattata tcaagcgtat
     3301 cattaaaaag tttaccttta tctctaactt t
//
LOCUS       DAOQWT010000124         3330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_30129_length_3330_cov_2.589924, whole genome shotgun sequence.
ACCESSION   DAOQWT010000124 DAOQWT010000000
VERSION     DAOQWT010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30129_length_3330_cov_2.589924"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PKJ95_03260"
     CDS             <1..392
                     /locus_tag="PKJ95_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR65297.1"
                     /translation="YAAQIAAIDNFIENELPQKYDTVIGERGIRLSGGERQRIGLARA
                     LYRNPEVLVLDEATSSLDGTTEEYVLKAIHSAAQSRTVIMIAHRLNTLKDCDQIYILE
                     KGRIIGQGKYEELISKNKKFREMAKMS"
     gene            608..892
                     /locus_tag="PKJ95_03265"
     CDS             608..892
                     /locus_tag="PKJ95_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR65298.1"
                     /translation="MKRIISKHYPYMKENFKIKEIGIFGSFVKNKADIDDISDIDILI
                     SFKKGHKDFFNYMRLKYYLEVILGRKVDLVIKDAIKPRLREKILQEVKYV"
     gene            1002..1241
                     /locus_tag="PKJ95_03270"
     CDS             1002..1241
                     /locus_tag="PKJ95_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008276776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNR65299.1"
                     /translation="MAVDAVIRNFEVIGEATKNIPREIKEKYPKVEWKEAAGFRDVLI
                     HDYFGIDLEALWDTIKKNLLPFKDNITTVLKNEEN"
     gene            1365..2423
                     /locus_tag="PKJ95_03275"
     CDS             1365..2423
                     /locus_tag="PKJ95_03275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR65300.1"
                     /translation="MRILFIAPMFHTNQMPWVQTLIENNHTVEYWSLCNKMESNVESL
                     KSINIKKIEQPYFNKIGAYYEQLKNFDPDVVVGRNPHLNLFILLFLLISKLQRRKTLI
                     YTQTDLYQVMPFSRKIGVTILLNTIGNYWITPLTGCRVNYPKFHCRAFYIPFIAPVST
                     QPQRNCNNINNNKINIISVGRINQKRKNVLLLLKALKLIEKNISFHLNLIGHMNKRGQ
                     LYEEITEFIKSNDLEDKVTIKENIPYHEMSLEYQKNNLFILPSSSEPAAYSLLEAMAA
                     GLPVICSDTNGTKCYIEEGENGYIFKSNNLEDLTEKIKIITSDKNEMKNMGERSAQIV
                     KEKYNTDSFYKAFEKLIQ"
     gene            2443..>3330
                     /locus_tag="PKJ95_03280"
     CDS             2443..>3330
                     /locus_tag="PKJ95_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR65301.1"
                     /translation="MALYILKRILSIMFSVIIVTIIIFSLMHSIPGGPFDEDKMPLSK
                     AAKEKIMKMYGLDQPLYIQYLKYIWNALHFKFGRSYQSPGEEMIDLINRTFRVSAFLG
                     GLGLFWAIPIGILLGIISAVKANSFIDYLFTLFSSYAISVPIYVTAIFMIFTFSLGLG
                     WFPTGGFEGLKTWVMPIIAYGLYPSGIIIRYTRNSMLEVMNEPYILTAKSKGLPLKSI
                     IVNHVFKNAMIPIITIILPIFARIITGSIFVETIFRIPGLGKYFVSSITQRDYPLMMT
                     LMLLVTFLLGFTYLSIDILY"
BASE COUNT         1248 a          445 c          563 g         1074 t
ORIGIN      
        1 aatatgcagc tcaaattgct gccatagata attttattga aaatgagttg cctcaaaaat
       61 atgacaccgt tataggagaa cgagggattc gccttagcgg tggggaaagg caaaggattg
      121 gattagctcg cgcactttat cgaaatcccg aagtattagt cttagatgaa gctaccagtt
      181 ccttggatgg cacaactgaa gagtatgtct taaaagcaat tcacagcgcc gcccaatcga
      241 gaacagtaat catgattgcc caccggctaa acacacttaa agattgcgac cagatttata
      301 tcttagaaaa aggcagaatc ataggacagg gtaaatatga agaactaatt agtaaaaata
      361 aaaaatttag agaaatggcg aaaatgtctt aatgcaatac ttaatgaaat aatacagaaa
      421 aataaataga acctactgac gacataagtc aggaaatcta ttttcttctt gattattctt
      481 ggttgataat cgttaataag gtaaaatata tataattata gaatatattt ctgtaccatt
      541 ttatagatct atttaaatag acctaaatcc aaatagattg gtggtggcaa ggttggatgt
      601 cagatatata aaaaggataa ttagtaagca ttacccttac atgaaagaaa actttaaaat
      661 taaagaaatt ggaatctttg gctctttcgt aaaaaataag gcagatatag atgatatcag
      721 cgatatagat atattgataa gttttaaaaa agggcataaa gattttttta attatatgag
      781 attaaaatat tatcttgaag tcattctggg gcgaaaagtg gatctagtca ttaaagatgc
      841 aataaaacca agattaagag aaaaaatact acaagaggta aaatatgtct aaaaaagaaa
      901 gagactacat tcttttttta gaagatattt tagaagccct tataaaaata gaaggttata
      961 tatgccggtg atatggattt taaaagtttt ttaaaaatga tatggctgta gatgcagtta
     1021 taagaaattt tgaggttatt ggagaagcaa ccaagaatat tccccgagaa ataaaagaaa
     1081 aatatcccaa agttgaatgg aaagaagcag ctggctttag agatgtttta atccatgatt
     1141 atttcggtat tgacctagag gctctatggg ataccataaa aaagaaccta ttacctttta
     1201 aggacaatat tacaacagta ttaaaaaatg aagagaatta ataataaaaa taatattcat
     1261 tcaagacata tatatacaac tacttataat atatttgtta ttttttgaat aaattgtatg
     1321 atattatatt aaaaaattaa taatttgaga gcataaaaag aattgtgaga atattattta
     1381 ttgcccctat gtttcatact aatcaaatgc catgggtaca aaccttaatt gaaaacaatc
     1441 atacggttga gtattggtca ctatgtaata aaatggagag taatgtagaa agtctaaaat
     1501 caataaatat taaaaaaatt gaacaacctt atttcaataa aataggtgca tattatgaac
     1561 aattgaaaaa ttttgatccg gatgtagtgg ttggtagaaa tcctcattta aatttgttta
     1621 tattactttt tttattaatt tccaaactgc agcgcagaaa aaccttgata tatacccaga
     1681 ctgatttgta tcaggtaatg cctttttcga ggaaaattgg tgtaaccata ctattaaaca
     1741 ctattggaaa ttattggatt acacctctta ctgggtgcag ggtaaattat ccaaaatttc
     1801 attgtagggc attttatatc ccctttatag cacctgtttc aacccaaccc caacgtaatt
     1861 gtaataatat taataataat aaaataaata ttatctctgt aggaaggata aatcaaaaaa
     1921 gaaaaaatgt tctattactg ttaaaagcgc tcaaattaat agagaaaaat attagttttc
     1981 atttgaacct gattggacat atgaataaaa gaggacaact ttatgaagaa attactgaat
     2041 ttattaaaag taatgatcta gaggacaaag taacaatcaa agaaaatatc ccctaccatg
     2101 aaatgtcctt agagtatcaa aaaaataatt tgtttatact tccaagctca tctgaacctg
     2161 ccgcttattc tcttttagaa gctatggctg ctggcttacc ggtaatttgc agtgatacga
     2221 atggtactaa atgctatatt gaagagggcg aaaacgggta catctttaag agtaacaacc
     2281 tggaagattt gactgaaaaa attaaaatta tcacctccga taaaaatgaa atgaaaaata
     2341 tgggagaaag aagcgcccaa attgtcaaag aaaagtacaa cacagattca ttctataaag
     2401 catttgaaaa attaattcaa taataaggtt aaaataaata agatggcact ttatatttta
     2461 aaaagaatct tatcaataat gttttcagta attatagtaa caataattat tttttctttg
     2521 atgcacagca ttccaggagg accgtttgat gaagacaaaa tgcctctatc taaagcagca
     2581 aaagaaaaaa taatgaaaat gtatggatta gaccagcctt tgtatattca atatcttaaa
     2641 tatatatgga atgccctcca tttcaaattt ggcaggtctt atcaaagtcc aggcgaggaa
     2701 atgattgatt taattaaccg aacttttagg gtcagtgcat tcttgggagg gttaggatta
     2761 ttttgggcaa taccgatagg tattttgtta ggaataattt cagctgttaa agcaaatagt
     2821 tttattgatt atctattcac tttatttagc agctatgcca tttctgtacc aatatatgta
     2881 actgctatat ttatgatatt tactttttct cttggacttg gttggtttcc gacaggagga
     2941 tttgagggac taaaaacatg ggtaatgcct ataattgctt atggtcttta cccttccgga
     3001 ataattattc gttatacccg taatagcatg ttagaggtta tgaatgagcc ttatatatta
     3061 actgccaagt ctaaagggtt gcctctgaaa tctattattg ttaaccatgt ttttaaaaat
     3121 gctatgattc caattattac gattatccta ccgatatttg ctagaattat tactggcagt
     3181 atttttgtag aaacaatatt cagaattcca gggttaggaa agtattttgt tagcagtatt
     3241 actcagcgtg attacccttt gatgatgact cttatgcttc ttgttacttt tttattaggc
     3301 tttacttatt tgagtataga tattttgtat
//
LOCUS       DAOQWT010000125         3321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_30226_length_3321_cov_3.156461, whole genome shotgun sequence.
ACCESSION   DAOQWT010000125 DAOQWT010000000
VERSION     DAOQWT010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30226_length_3321_cov_3.156461"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..506
                     /locus_tag="PKJ95_03285"
     CDS             15..506
                     /locus_tag="PKJ95_03285"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HNR65302.1"
                     /translation="MVLSGCPVKRGRFVGKEPARYRCSAEALLRRVVQGKEIYQINNV
                     VDIINLISLLFHFSIGCYDFNRLREPIEFDIGEEKESYHAIGRGIMNIAHLPVFRDKI
                     SAFGSPTSDSERTMITTKTEKVLIAIIDFKSSDILPDAMEKTIDCLRQYADAKNPQSL
                     IIN"
     gene            complement(496..1401)
                     /gene="dapA"
                     /locus_tag="PKJ95_03290"
     CDS             complement(496..1401)
                     /gene="dapA"
                     /locus_tag="PKJ95_03290"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNR65303.1"
                     /translation="MAGLDFNGIFPPIITPFQKEEIAYNYLTENMEKLNKSGIKGVVV
                     LGSNGENVFLSEEEKISVVSTVIKAVSKDMVIIVGSGCESARETIYLTNKLAEIGAQA
                     ALIVTPFYYGSKMTDEALIKYYSTVADNTDIPVLLYNVPKFTGVNLGIEALAVLAKHP
                     NIIGIKDSSGNVNQLGQYLNKVDNSFSILVGTAGALLGALVLGCKGGILALANLVPEK
                     CVELYQLVQEGKLKEARELQLRMIPVNHATTVSYGISGLKYAMDLLGYKGGETRLPLL
                     PLKKEEKEKIRNILLEAELKINTNL"
     gene            1660..>2094
                     /locus_tag="PKJ95_03295"
     CDS             1660..>2094
                     /locus_tag="PKJ95_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HNR65304.1"
                     /translation="MYYSEIAGVGSYVPEKMVSNVDLEAFIDTTDQWITTRTGIAQRR
                     ISTGEKASQMAVKAAVKAIKHAAIPIEDIDLVILATITPDFFTPSTASLVQAELKLKN
                     ITSFDISAGCTGFIYGIQIADQFIKSGKSNCALVIGAEILSKV"
     assembly_gap    2095..2104
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2105..2650
                     /locus_tag="PKJ95_03300"
     CDS             <2105..2650
                     /locus_tag="PKJ95_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNR65305.1"
                     /translation="NWQDRNTCVLFGDGAGAVILKRSNREGILYTYTGSSGDLEGLLT
                     LPAIPLKNPFYIDNPISDQPSYIYMNGKEIFKFATNIMLQSIERVLKETKLTIADIDF
                     IIPHQANFRIIDYVAKKIKAKTGQFYKNLDRFGNTSAASIPLALDEMVAKGMLQKGNR
                     VIMVGFGGGLTWGSILFNWVR"
     gene            2663..>3321
                     /locus_tag="PKJ95_03305"
     CDS             2663..>3321
                     /locus_tag="PKJ95_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNR65306.1"
                     /translation="MIYTVINELLNIDYPIIQGGMAGIADATLAAAVSNAGGLGVIGA
                     GYASEEWIRGEIRKTRLLTKKPFALNIMLLNPEAEMVGKVAMEEGIEVIITGAGSPGK
                     YISSWKSKGIKVIPVIPSVSIAQRMEKMGADAVIAEGGEAGGHIGEMNTMALIPQVVD
                     SVTVPVIAAGGIGDGRGVAAALMLGATGVQLGTRFLVALECNISRAYKEKILQAKDTS
                     TT"
BASE COUNT         1045 a          575 c          685 g         1006 t
ORIGIN      
        1 atatagtata aattatagtc ttatctggtt gtccagtaaa gcgggggagg ttcgtaggga
       61 aagagccggc acggtatcgt tgttctgcag aagcattgtt acggagggtt gtacagggaa
      121 aggaaatata ccagattaat aatgttgttg atattattaa tcttatctcc ctcttatttc
      181 atttttccat agggtgctat gattttaata gattaagaga accgattgaa tttgatattg
      241 gagaagaaaa ggaaagttat catgccattg gcagagggat tatgaatatt gctcatttac
      301 cggtttttag agataagata agtgcctttg gcagtcctac cagtgattcg gaaaggacga
      361 tgattactac caaaaccgaa aaagttttaa tcgccattat cgattttaaa agtagtgata
      421 ttcttccaga tgcaatggaa aagactatag actgtttaag acaatacgct gatgcaaaaa
      481 atcctcaaag tttaattata aattagtatt aattttcaac tcagcttcta ataagatgtt
      541 tctaatcttc tctttttcct ctttcttaag aggtaaaagc ggcagcctgg tttcccctcc
      601 cttataaccc aataaatcca tggcatattt tagaccgctt ataccataac tgacagtggt
      661 ggcatggtta acaggtatca ttcttaactg aagttccctt gcctccttta gtttaccttc
      721 ttgcactaac tgatagagtt ctacacattt ttccggcaca agattagcca gagccagaat
      781 tccacccttg catcccagaa caagtgctcc caacaatgct cctgctgttc ccaccaggat
      841 actgaaacta ttatcaactt tgtttagata ttgacccagc tgattgacat ttccactgct
      901 gtctttaata ccgattatat tggggtgttt ggccaataca gccaatgctt caatgcccag
      961 gttaacgccg gtaaattttg gtacattgta taacaaaact ggtatatcag tattatcggc
     1021 cacggtggag taatatttta tcaatgcctc atcagtcatc ttgcttccat aataaaaagg
     1081 agtaacaata agggcggcct gtgcacctat ctctgctaat ttattggtaa gatagatggt
     1141 ttctcgggca gattcacaac ctgaaccaac tataataacc atatctttag agacagcttt
     1201 aattacagtt gaaactacac tgattttttc ctcttcggaa agaaatacat tttccccatt
     1261 ggaacccagg actacaactc ctttgatacc actcttattt aatttctcca tattttcggt
     1321 tagataatta taagctatct cttctttttg gaaaggagta ataataggcg ggaaaattcc
     1381 attaaaatca agaccagcca taatcaaacc tcctgattaa tcataagata taagataaaa
     1441 aatttttaaa acatggctaa ttaaagtata agagaaaata tttttttagt aaaattattt
     1501 tttatatttt ttatttaata tcattgacag aatgttaaag gaaatatata ctaactatta
     1561 ttataagtta ctaatagaca ctactaatag tagttactaa taataacgtt tctaaagaat
     1621 cgcaagataa aaagaaaagg tatacacaga ggtgtggcaa tgtattacag tgaaattgca
     1681 ggagtgggca gttatgtacc ggagaaaatg gtcagtaatg tagatttaga ggccttcatt
     1741 gataccactg accagtggat tactaccagg accggaattg cccagcgcag aatctctacc
     1801 ggtgagaagg cttcccaaat ggcggtaaaa gcagctgtca aagccattaa acatgccgct
     1861 atacccattg aagatatcga tctggtaata ctggctacta ttacccctga tttttttact
     1921 ccttctacag caagcctggt tcaggcagag ttgaagctga aaaatattac cagttttgat
     1981 atttctgccg gttgcaccgg ctttatttat ggaatccaga ttgcagatca atttataaaa
     2041 tctggaaaaa gtaattgtgc tctggtgatt ggcgcagaaa tattatccaa ggtgnnnnnn
     2101 nnnnaactgg caggatagaa atacctgcgt cttatttgga gatggggctg gggctgttat
     2161 tttgaaaagg agtaacagag agggaatttt gtatacctat accggttcca gtggagacct
     2221 ggaaggtcta ttaacgttac cggccatacc cctaaagaat cctttttata tagataatcc
     2281 tatatcagac caaccttctt atatttatat gaatggaaaa gagattttta aatttgctac
     2341 caatattatg ctacagagca ttgaaagggt tttaaaagaa actaaactaa ccattgctga
     2401 tattgatttt ataattcctc atcaagctaa ttttcgcatt attgactatg ttgccaaaaa
     2461 aattaaggca aaaacaggtc aattttataa aaacttagac cgctttggaa atacctctgc
     2521 tgccagcatt cccttagcct tagatgagat ggtagctaaa ggaatgttgc aaaaaggcaa
     2581 tcgcgtaatt atggtcggtt ttggcggtgg gttgacctgg ggttcaattc tctttaactg
     2641 ggttagataa ggagtattta gaatgatata tacggttatt aatgagttac ttaatattga
     2701 ttatcccata atacagggcg gtatggcagg aatagccgat gcaacacttg ctgcagcggt
     2761 ttccaatgcc ggaggattgg gtgtaatagg tgcgggatat gcctcagaag agtggataag
     2821 aggagaaatc aggaaaaccc gattattaac taaaaaaccc tttgccttaa atattatgct
     2881 cttaaatcct gaggcagaaa tggttgggaa ggtggctatg gaggaaggga tagaagtcat
     2941 tattactgga gcaggaagtc ctggtaaata tatttcatcc tggaagagta agggaataaa
     3001 ggtcatccct gttattcctt cagtatcgat cgcccaaaga atggaaaaaa tgggagcaga
     3061 tgctgttatt gctgaaggag gagaggcagg ggggcatata ggggagatga atactatggc
     3121 tttgattccc caggtagtcg attcagttac tgttccggta attgctgctg gtggaatagg
     3181 tgatggccgg ggagtggcag cagctttaat gctgggtgcc acgggcgtac aattgggaac
     3241 ccggttttta gtagctctgg aatgtaatat ttctagagca tataaagaaa agattttaca
     3301 ggccaaagat accagtacca c
//
LOCUS       DAOQWT010000126         3278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_30826_length_3278_cov_3.277381, whole genome shotgun sequence.
ACCESSION   DAOQWT010000126 DAOQWT010000000
VERSION     DAOQWT010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30826_length_3278_cov_3.277381"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="PKJ95_03310"
     CDS             <1..1495
                     /locus_tag="PKJ95_03310"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNR65307.1"
                     /translation="DQIIIRKGEIVTDEHIERLEALGLQNPIIRFKEIFGLFLINTLF
                     ISLLSLYIFNYHQEIYNDFNKILLLGVIYLSTIFIAKISSQISGYIIPVAFASMLIAI
                     TLDSRLALEVTFLICINIGFIFLGEIDYMVVALAGGLISIFSIRKATQRSSLTRAGLL
                     IALINVVSIIALGLIHRNPADYILQNSMWGLLNGFASAILTIGILPFLETFFDISSSF
                     RLMELTNPNQPLLKQLLVDAPGTYHHSVVVGNLAEAATEEIGGNALLARVGAYYHDIG
                     KLKRPYFFAENQEAYKNIHDDLEPSLSALVIASHVKEGVDMARKFKLPRSIIDIINQH
                     HGTGIISYFYHRALQTNTDKSSEVDEESYRYSGPKPQSKEAGVILLADMLEAEARTLN
                     NPTASRIKNLTQNVINRNLVNGQLDDCNLTLRELNKIKDVFSRVLTGMFHNRVEYPDE
                     ELINKLKRDRAQSESVNKKQTETSTRSVRNEKNFADSDEIYQTKKNK"
     gene            1440..1877
                     /gene="ybeY"
                     /locus_tag="PKJ95_03315"
     CDS             1440..1877
                     /gene="ybeY"
                     /locus_tag="PKJ95_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNR65308.1"
                     /translation="MKKTLLTAMKFTKQKKTSEVSLLLTDDKGIQRLNKKYRNIDSPT
                     DVLAFSQIENGNSNRVSLLHKKEEYLLGDIVISVETAQRQSLNLGHSLQYELILLLIH
                     GFLHLLGFEHDSESKYEQMKALEKEVFNVILKDTKKHNKYPNQ"
     gene            2137..3093
                     /locus_tag="PKJ95_03320"
     CDS             2137..3093
                     /locus_tag="PKJ95_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNR65309.1"
                     /translation="MKKFITFLVISVLILTFSISGIAQQKFLAIATGGTGGTYYPLGG
                     ALAQLLSNKVEGLIVTAQTSGASIANCNLIARHQIETAFSQANTTYWSYTATGLQAGQ
                     QPITNLRGIASLYPETIHIVATKASGVKTIADLKGKRVGVGAPNSGTEADARIILNAH
                     GLTYDDMSVDYIDFNEVADRLIDGQIDAGFVTAGYPTSSIINIATVRDLVIVPVDREV
                     SEKLIKEIPYYGLTEIPAGMYKGVEEPVLSLATPALWICDSQLDPTLVYKMTKALWEN
                     VNILAIVHAQGKNITLETALSGMAIPLHPGAELYYKEIGLIK"
BASE COUNT         1088 a          520 c          590 g         1080 t
ORIGIN      
        1 ggaccagata attataagaa agggagaaat tgttaccgat gaacatattg aaaggttaga
       61 agctttagga ttacaaaatc ctattattcg tttcaaggaa atttttggtt tatttctaat
      121 caatacattg ttcatctcct tattaagctt atatattttc aactaccatc aagaaatcta
      181 taatgatttc aataaaatat tattattagg ggtaatttat ttatctacaa tttttattgc
      241 taaaatcagc agccagatat ccggatatat tatacctgtc gcctttgcct ccatgcttat
      301 tgctatcact cttgattcca gattggcttt agaagtcaca ttcctaatct gcattaatat
      361 cggatttata tttttaggtg aaattgatta tatggtagta gccttggcag gggggttaat
      421 ttctattttt agtattcgaa aagctactca gcgctccagc ttaacccgtg ctggcctttt
      481 aattgccctg attaatgttg tatctattat agctttgggc ttaatacaca ggaatcctgc
      541 tgattacata ttgcaaaaca gtatgtgggg tttattgaat ggctttgcat cagctatttt
      601 aaccatagga attcttcctt ttttagaaac tttttttgac atatcttctt catttagact
      661 tatggaattg actaatccca accagccact attaaaacaa ttactggttg atgctccagg
      721 aacttatcat catagcgtgg ttgttggcaa tctagctgaa gctgccactg aggagattgg
      781 gggtaatgcc ctcttagcac gtgtaggcgc ttactatcat gatataggaa aattaaagag
      841 accctacttt tttgctgaaa accaggaggc ttataaaaat attcatgatg atttagagcc
      901 aagcttaagc gctttggtca tagcttctca tgtcaaagag ggagtggata tggctagaaa
      961 atttaaattg cccagatcaa ttattgatat aataaatcaa caccatggaa caggaattat
     1021 cagttatttt tatcaccgtg ccttgcaaac aaataccgat aaatctagtg aggtggacga
     1081 agaaagctat cgctattcgg gtccaaagcc tcaaagcaaa gaggcagggg ttattttact
     1141 tgccgatatg cttgaagctg aagcgagaac tttaaataat cccacagcca gccgcatcaa
     1201 gaacttaaca cagaatgtta ttaacagaaa cctagttaat ggtcaacttg atgattgcaa
     1261 tctcacttta agagaattaa ataagattaa agatgttttc tccagagtat tgaccggaat
     1321 gtttcataat agagttgaat atcccgatga agaattaata aataaattaa agagggaccg
     1381 cgctcaaagt gaaagtgtta ataaaaaaca gacagaaact agcactagat ctgtcagaaa
     1441 tgaaaaaaac tttgctgaca gcgatgaaat ttaccaaaca aaaaaaaaca agtgaagtta
     1501 gtctattact aaccgatgat aaaggaatac aaaggttaaa taaaaaatat cggaatatag
     1561 attcccccac cgatgtactt gctttcagtc aaattgaaaa tggaaatagt aatagagttt
     1621 ccttacttca taagaaagag gaatatttat taggggatat tgtgatttct gttgaaactg
     1681 cacaaagaca gtccctcaac ctcggccatt ccttacaata tgagttaata ttgctcttga
     1741 ttcatggttt cctgcatctt ttgggatttg aacatgatag tgaaagcaaa tatgagcaaa
     1801 tgaaagcttt agaaaaagaa gttttcaatg ttattttgaa agatacaaag aaacataata
     1861 aatatcccaa tcaataatat atttatttaa tttgttattt attaataaaa tgtactatca
     1921 agttttcttc tattgaaatg ccattaattt tactgtatcg tttaaaattt agttatgtaa
     1981 aatttatgaa aagttgaact ttactttaaa ataaggtaaa ctatcagtat atataaaggt
     2041 aaacctaaaa ataataatag tagaggaggg ataaaaaaag gttaatcata tatctatttt
     2101 ttaaattaat aaattttatg gaggttttct aagtttatga aaaaattcat tacgttttta
     2161 gttatcagtg ttttgatttt aacatttagc ataagtggca tagctcaaca aaaatttctg
     2221 gccattgcta ctggcggtac gggaggaact tattatcctt tgggcggtgc tttggctcaa
     2281 ttgctttcca ataaagttga aggtttaatt gtaactgcac agacttcagg tgcctcaatc
     2341 gctaattgta atttgattgc tcgacatcag atcgaaaccg ctttttcaca ggccaatacc
     2401 acctattggt cctatactgc taccggactt caagctggtc agcaaccgat tacaaattta
     2461 agaggaatag cctctctata ccctgaaaca attcatattg ttgctaccaa agcttctgga
     2521 gtaaagacaa ttgcggattt aaaagggaaa agagttggag ttggtgcacc caatagtgga
     2581 actgaagcag atgccagaat tattttaaat gctcatggtc ttacctatga tgatatgagt
     2641 gttgattata ttgatttcaa tgaagtggca gaccgtttga tcgatgggca gatagatgcc
     2701 ggttttgtta ctgctggtta tcctacttcc agtattatta atattgctac agtacgtgac
     2761 cttgttatag ttccagtaga cagagaggtg tctgaaaagt taatcaaaga aataccttat
     2821 tatggtctta ctgaaatccc tgccggtatg tataaaggtg tagaagaacc cgttttatct
     2881 ttagctactc cggctctttg gatatgcgat tcacagttag acccaacatt agtctataag
     2941 atgaccaaag ccctctggga aaatgttaac attcttgcta ttgtacatgc ccagggtaaa
     3001 aatatcactt tagaaacagc gctttctggt atggctattc cgcttcatcc tggtgctgaa
     3061 ctttattata aagaaatagg gctaataaaa taataatcta ctattagttg ttattaataa
     3121 agtccggata ctattagagc aggatgatgg tatccggact tctttgtttg atattatttt
     3181 ttaattaaga agtttaatgc aaatttaaaa aattagattc tatgaagaat aaaccacatt
     3241 tttatttgcc ccgttttgtg ttattattat tgttgata
//
LOCUS       DAOQWT010000127         3276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_30847_length_3276_cov_3.263272, whole genome shotgun sequence.
ACCESSION   DAOQWT010000127 DAOQWT010000000
VERSION     DAOQWT010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30847_length_3276_cov_3.263272"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            423..560
                     /locus_tag="PKJ95_03325"
     CDS             423..560
                     /locus_tag="PKJ95_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65310.1"
                     /translation="MWEIEYEIATSAIGRLAMTNTKKQRKNTKDVGINGRSHWPLVDI
                     I"
     gene            complement(671..2134)
                     /locus_tag="PKJ95_03330"
     CDS             complement(671..2134)
                     /locus_tag="PKJ95_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016764099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNR65311.1"
                     /translation="MVGIDKDFLAHPVLYFFAEISKIPRCSGNEKAISDYLVSFARER
                     NLEVEQDKSLNVIIKKPAFPGYENAPTVIIQGHMDMVCDKNKSKEHNFETDPIELRVV
                     DDMLYATDTTLGADNGIAVAYAMALLDSDDIPHPPLKVLLTTEEETGLKGAAKLDPAY
                     LEGDMLINIDSEEEGVLFSSCAGGIRVSHFIEAVWENPAPDNISYLVEIKGLKGGHSG
                     AEIDKSRGNANKLMGRFLSALSDEIEFQIGKINGGSKMNVIPREAEAVLFLHPNQGKI
                     LFAKIEEWNTIFRKEFRSKDPDIVLNASKTTEKDTKVLSKEITEKVINLLVIMPDGAQ
                     TMSGDIEGLVESSTNLGVVSTSDSGISFESATRSNIGSQKHNILNRLKTIAYIFNIDF
                     KTDADYPEWEYQADSKLREIFKKVYKDMYQKELKITAIHAGLECGFFKKKNKNLDIVA
                     LGPDQYDVHTPDEHVSIPSLKRTYEYLLNVLKHIKPS"
     gene            complement(2526..>3276)
                     /locus_tag="PKJ95_03335"
     CDS             complement(2526..>3276)
                     /locus_tag="PKJ95_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65312.1"
                     /translation="EKDSPVVRDIETFVSYFLENRIKVTRVNRWIPFKHLKIITKNFI
                     SPDRDEFIDIGYEECWGQEQEKIYIHFIHLLAQSAQLIYTDKQGYVQVNQDHFKNFTE
                     NNYGENVFKLLIDWIEKVNWEDLYPAAQVKPYAQEYQGKIVGLWPSIHELKPYERIDT
                     ISFTKYLYYDDRKGEEELEEIARELSMIVDSVLLRYLNWFGVIDAQKVKYHPRLLGWT
                     IKEFWITNNGRKLADRVVLDFWEKGKIKMKK"
BASE COUNT          965 a          565 c          586 g         1160 t
ORIGIN      
        1 tttgcttatt tgattcaaat tgagccagac agtttcttct tttaagttta cttcgaatgt
       61 ttcttttcca tcttctgtgg tataaatgat aatttcattt tctctgggca tttttaatta
      121 tatccctttc agattaataa tatttttatt ctaccagaaa tttgtttata tttcttgctt
      181 tgtctgtcta attgcatgtt attgttattg attgttttgt cgtgaaggat tttgcaggga
      241 tgtaaagatt aaatatgtgg ggataaatga agatggtcgt tggtcgtttg tcgttggtat
      301 ttagtggaaa tacgaagaga aagactgggg gataaatgag agaggttttt ggtttatggt
      361 ttttagtaaa aatacaaata taaaagaaga gtgtgggtat acatggtggt ttaaagataa
      421 agatgtggga gatagagtat gagattgcca cgtctgccat cggcagactt gcaatgacaa
      481 atacaaagaa acaaagaaaa aatactaaag atgttgggat aaatggaaga agtcattggc
      541 cgttagtcga cattatttag tgaaattata aagaaaaaat aaatgcaaag agaagaatgt
      601 gggaatagag ggtgggattg ctaaacctgc tcttaacaga agttaagagc aggttcggaa
      661 tgacaaaaaa ttaggatggt ttgatgtgtt ttaggacgtt gagaaggtat tcataggttc
      721 tttttaaaga tggtatgctt acatgctcat caggagtatg tacatcatac tggtctggtc
      781 ctaaagcgac tatatccagg tttttattct tttttttaaa gaaaccacat tccaatccgg
      841 catggatggc tgtaattttc agctccttct ggtacatgtc cttataaacc ttcttgaata
      901 tttctctcag ttttgaatca gcctggtatt cccattctgg ataatcggca tcagttttaa
      961 aatcaatatt aaagatataa gcaatagttt ttaatcgatt gagaatatta tgcttctggc
     1021 taccgatatt actcctggta gcactttcaa aggatatgcc tgaatcagaa gtagagacta
     1081 cgcccagatt agtagagctt tctaccagac cctcgatatc tccgctcatt gtttgtgctc
     1141 catctggcat aattacaagt agattaatta ctttttctgt tatctcttta gataatactt
     1201 ttgtatcttt ttctgtggtt ttgctggcat taaggactat atccgggtct ttgctgcgaa
     1261 attctttcct aaaaattgta ttccattctt ctattttggc aaaaagtatc tttccctgat
     1321 taggatgaag gaataataca gcttctgctt ctcgaggaat gacattcatt ttggaaccac
     1381 cgttgatttt accgatttga aattctatct catctgaaag tgcacttaaa aacctaccca
     1441 taagtttatt ggcatttcct ctgctcttgt ctatttctgc tccagaatgg ccacctttta
     1501 atccttttat ttcgaccaga taggaaatat tatctggtgc cggattctcc catactgcct
     1561 cgataaaatg agatactctt atcccccctg cacaactaga aaatagaaca ccctcttctt
     1621 cactatcgat attgatgagc atatcacctt ccaggtatgc agggtctaat ttggctgccc
     1681 cttttaaacc ggtctcttct tctgtggtta gaagtacctt gagcggagga tgtggtatat
     1741 cgtcagaatc taaaagggcc atggcatagg caactgctat accattgtct gcccctaagg
     1801 tggtatctgt ggcatagagc atatcatcta ccacacggag ttcaatgggg tctgtctcaa
     1861 agttatgctc ttttgattta ttcttatcac ataccatatc catatgtccc tgtatgatta
     1921 ccgttggtgc attttcatat ccaggaaagg caggtttttt gattatgaca tttaatgatt
     1981 tatcctgttc tacttcaaga tttctctctc tggcaaaaga taccagataa tcacttatcg
     2041 ccttttcatt tcctgaacat ctgggaatct tgctaatttc tgcaaagaag taaagaacag
     2101 ggtgtgctaa aaaatcctta tctattccaa ccatataata taatcccttt cttttaatat
     2161 atttttatta taaagttcgg ggctaatatt ttcaattttt tcaatcatat acatttaata
     2221 taaattagtt tgttttaaac attgactctt tttattgaat gtgttgaaaa gttaatggtt
     2281 tttggttttt ggagtaaatg taaaacaaag agtgcgggct acagagtatg agactgccat
     2341 tcactgctaa cacagcaatg ctgaatatta aaataatgta ggggtaattc tagagatatc
     2401 aaagacatac tttcagcaaa tcttgcaata acgggggaat taattaattg caatgacgaa
     2461 aaagaataaa tacttggtat gatagaaaag aatgctttaa taatagtaga aaagaaaagg
     2521 gaaggtcatt ttttcatttt aattttgcct ttttcccaaa aatcaagcac tactctgtct
     2581 gctaattttc taccattatt ggtaatccag aattctttga tggtccaccc tagcaacctg
     2641 ggatgatact tcaccttctg tgcatctatt actccaaacc aatttaaata cctcaacaag
     2701 actgaatcta caatcatact taattctctt gctatttcct caagctcttc ctctcccttt
     2761 ctgtcatcat agtagaggta tttagtgaaa gatattgtat cgatcctttc atagggcttt
     2821 aattcatgga tggagggcca taatcctaca atttttccct ggtattcctg ggcatatggt
     2881 ttaacctgtg ctgctggata taaatcttcc cagttaactt tttcaatcca gtctatcagc
     2941 agtttaaaaa cattttcacc atagttgttt tcagtaaaat ttttaaaatg atcttggttc
     3001 acctgtacat atccttgttt atctgtataa attaattgag cagattgtgc taaaaggtga
     3061 ataaagtgaa tatagatttt ttcttgttcc tgaccccagc attcttcata tccaatatct
     3121 ataaactcat ctctatctgg actaataaaa tttttggtaa taattttcag gtgtttaaag
     3181 ggaatccagc gattaacccg ggtaacttta atacggtttt ccagaaaata gctgacaaag
     3241 gtctctatgt ctcgcactac tggagaatct ttctcg
//
LOCUS       DAOQWT010000128         3275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_30865_length_3275_cov_2.510248, whole genome shotgun sequence.
ACCESSION   DAOQWT010000128 DAOQWT010000000
VERSION     DAOQWT010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30865_length_3275_cov_2.510248"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PKJ95_03340"
     CDS             <1..726
                     /locus_tag="PKJ95_03340"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNR65313.1"
                     /translation="GLFAVLLANCFKYNHLDTGSIFSCGFFSNTVSLGGMICFFYLGE
                     EGYALVPIYTFLMRLFYFGAGYPIARMYGSCNSPEENITKKLLAILKDPFFYIGVGAV
                     LLGLLLNISSLPRPKIYGYVNEMLIPISTFILLFSVGLNLKLSRVSRYIKESLYMSII
                     KFIAVPLITLAIVLLLNYQSIGNGLPLKVSLIMSSMPVAFNSVIAANIYNLNVDLVNA
                     CWIFTTIGIIFFLPLLFFFINLF"
     gene            860..1117
                     /locus_tag="PKJ95_03345"
     CDS             860..1117
                     /locus_tag="PKJ95_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNR65314.1"
                     /translation="MKVKLIVYSNLKNYIDGYQDSEGLLKEISKQMTIKDFLQENIKH
                     KRAMDGISMVIVNEKVIPFHEIERNLQDGDVIKIYPPMGGG"
     gene            1212..1751
                     /gene="msrA"
                     /locus_tag="PKJ95_03350"
     CDS             1212..1751
                     /gene="msrA"
                     /locus_tag="PKJ95_03350"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNR65315.1"
                     /translation="MEEKKEVATLAGGCFWCLEAIYKRVKGVLEVVSGYSGGNIKNPT
                     YEQVCSDRTGHAEAIQIIFNPEIISYDEILKIFWKIHNPTTHNRQGNDVGSQYRSMIF
                     YHNEKQKEIAESSKKDLQDAHIWHDSIVTEIVPFIEFYPAEEYHQDYYENNPHNSYCQ
                     FVVGPKIKKFVKECKEYLK"
     gene            1775..2509
                     /locus_tag="PKJ95_03355"
     CDS             1775..2509
                     /locus_tag="PKJ95_03355"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNR65316.1"
                     /translation="MVKEKNFQSNNYTAAFLIMGISIVLAALVFGFFFYQTRSSRDYV
                     QVLGAATQGFESDIVKWNLTLEESTDLHNVIDGYRNIQLKRKKLIDILVNKGISNDDI
                     NINPISSYNRWGREGEMTGYTLNQSLFIISTEIEAIETLALNPDELLDQEIFFQTSSL
                     EYFYSKIDDMKKDLLELATRNARERADMILKESNHKAGKMIWSQAGVFQIIEPYSTEV
                     ESYGMYNTASRKKEIKVTVHANFLIQ"
     gene            2699..>3275
                     /locus_tag="PKJ95_03360"
     CDS             2699..>3275
                     /locus_tag="PKJ95_03360"
                     /inference="COORDINATES: protein motif:HMM:NF025757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HNR65317.1"
                     /translation="MRIILNLPHRKQKSTCYINGLYDILLWKNVEYDYFLLPVIGGMA
                     GFAYIKFRGANPPHIVFWGNRPKNLFNELEPLLKYKHKIVEGKSWKTTFRKIKESLNH
                     NIPIVAGALDMYFLPYYKNLYKQAHIPIHYVLIIGYDQEKEEFYIHDCSYNEVKTIPY
                     SNLKCSMDIKVPGMSRKNTIRMFKFAEELPDE"
BASE COUNT         1159 a          453 c          589 g         1074 t
ORIGIN      
        1 ggattatttg ctgttctatt ggcaaattgt tttaaatata accatttaga taccggttca
       61 attttcagct gcggtttttt ttctaatact gtttcccttg gtggtatgat ctgttttttc
      121 tatttaggcg aggaaggata tgccttagta cctatttata ctttcttaat gcgtttgttt
      181 tactttggtg caggatatcc tattgcccga atgtatggca gttgcaatag tcctgaggag
      241 aacattacta aaaaattgct agcaatactt aaagaccctt ttttctatat aggcgtaggt
      301 gctgtactcc tgggattgtt gcttaatata agttctttgc cccgccccaa aatatatggt
      361 tatgttaacg agatgcttat tcctatttca acctttatat tattattttc agtgggcctt
      421 aatttgaaat taagtcgtgt tagccgttac atcaaagaat ctttatatat gtcaataatt
      481 aaatttatag ctgtaccatt gattacactg gcaatagttc ttttgcttaa ctatcagtct
      541 ataggtaatg gacttccctt gaaagtttct ctgatcatgt cttcaatgcc ggtagccttc
      601 aattcagtta tagctgcaaa tatttataat ttaaatgttg atctggtcaa tgcctgctgg
      661 atttttacta ccataggtat catatttttt ttacctctct tgttcttttt tataaatttg
      721 ttttagaaca atatatctgt atgaaatgct aaaatatgtt ggtttggaaa taattcttta
      781 aaactatttt tgtgagcatc acattataaa aatcacatta taaaaattaa agaattaata
      841 ctattaaatg ggagaaggaa tgaaggtaaa gttgattgta tattctaatt tgaaaaacta
      901 tatagatggt tatcaagata gtgaaggttt gttaaaagaa atttcaaaac aaatgaccat
      961 taaggatttc ttacaggaaa atatcaagca taaaagagct atggatggta tcagtatggt
     1021 aatcgtaaat gaaaaagtta ttcctttcca tgagatagaa cgcaacctgc aagatgggga
     1081 tgtaattaaa atttatcctc ctatgggtgg tggttaaaga taataaatgt tagataaata
     1141 tatgattcta tttaatcctt tttattggta taattatgat atctaaataa taagacggga
     1201 ataagtgtaa aatggaggag aaaaaagaag tagctacttt agctggtggt tgtttttggt
     1261 gtctggaggc aatttataaa agagtaaagg gtgttctgga agttgtttcc ggttatagtg
     1321 gtggtaatat aaaaaatcca acctatgaac aggtctgttc tgatagaacc ggccatgctg
     1381 aggcaattca aataattttt aatcctgaaa ttatttccta tgatgagata ttaaaaatat
     1441 tctggaaaat tcacaatccc accacccata atcgacaggg caatgatgta gggtctcaat
     1501 atcgttcaat gattttttat cataatgaaa aacagaaaga aattgctgaa agttcaaaaa
     1561 aagatttaca ggatgcccat atatggcacg attctattgt aacagaaata gttcctttta
     1621 tagaattcta tccggcagaa gaatatcacc aggattatta tgagaataat ccccataata
     1681 gctattgtca gtttgtggta ggacccaaga taaaaaaatt tgtcaaagaa tgtaaagaat
     1741 accttaaata gtaacaggaa aaaggaggag taaagtggta aaggagaaaa attttcaatc
     1801 taataattat acagctgcat tcctgataat gggaatatct attgttttag ccgcactagt
     1861 atttggattc ttcttttatc aaaccagaag cagccgtgat tatgttcagg tattgggtgc
     1921 agcaactcag ggatttgaat cagatattgt gaaatggaat ttaactctgg aggaaagcac
     1981 tgatttgcat aatgttatag atggttaccg aaatattcag ctaaagagaa aaaaattaat
     2041 cgatatattg gtaaataaag gaatatcaaa tgatgatata aacattaacc ccatttctag
     2101 ttataaccga tggggccggg agggggagat gacgggttac accttaaatc agtcactttt
     2161 tataatctct acagaaattg aggctattga gactttagcc ctaaatccgg atgaactttt
     2221 agatcaggaa atctttttcc agacttcatc tttagaatat ttttactcca aaattgatga
     2281 tatgaaaaaa gatttattgg aattagccac cagaaatgca cgggaaaggg cagatatgat
     2341 tttaaaggag agcaatcata aagccgggaa aatgatctgg tcccaggcag gtgttttcca
     2401 gattattgaa ccatattcta ctgaggtaga aagttatgga atgtacaaca ctgcatcccg
     2461 gaaaaaggag attaaagtga ctgttcacgc caacttttta attcaatagg aattgagtaa
     2521 aaattataag ataaatttaa ataacgctag ataaattatt ttataaaagc tcaccagtcg
     2581 caacttcaaa tgaatctctt caagttatat ttttaaaaat tatctttaaa taaagattag
     2641 tcaaaggtaa ttagataaaa gaattacata ttttttaaaa ttgtattaaa tgtaaaaaat
     2701 gagaataata ttaaatctac cacataggaa acagaaatca acctgctata ttaatggatt
     2761 atatgatatt ctgttatgga aaaatgttga atatgattat tttcttttac ctgtaatagg
     2821 gggtatggca ggatttgctt atataaaatt tagaggggca aatcccccac atatagtttt
     2881 ttggggtaac agaccaaaaa atttgttcaa tgaattagaa ccacttttaa aatataagca
     2941 taaaatcgtt gaaggaaaga gctggaaaac aacttttaga aaaattaaag aatctctaaa
     3001 tcacaatata ccaattgtag cgggagctct agacatgtac ttcctgcctt attataaaaa
     3061 tctatacaag caagcacaca ttcccataca ctatgtcctt attataggct atgaccagga
     3121 aaaggaagaa ttctatattc atgattgcag ctacaatgaa gtaaaaacaa taccgtatag
     3181 caatttgaag tgttccatgg atataaaagt accagggatg agcaggaaga atactataag
     3241 gatgtttaaa ttcgcagaag aattaccaga tgaac
//
LOCUS       DAOQWT010000129         3269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_30935_length_3269_cov_3.109521, whole genome shotgun sequence.
ACCESSION   DAOQWT010000129 DAOQWT010000000
VERSION     DAOQWT010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30935_length_3269_cov_3.109521"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKJ95_03365"
     CDS             <1..195
                     /locus_tag="PKJ95_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HNR65318.1"
                     /translation="AGAKGLMVGDAQVSTKHANFILNRGHATASDILRLIEKIEKMVE
                     KTFGIKLEREIDFIGLPGYR"
     gene            164..973
                     /locus_tag="PKJ95_03370"
     CDS             164..973
                     /locus_tag="PKJ95_03370"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNR65319.1"
                     /translation="MILSGYRVTDNYENEEEILDEDGEEDSREYNLFRVLLFYIIVGF
                     LSWNIFYFVFTSSWFDIKRVTIHGNNYLDSDTILTQGELDKPVNIFHFNTEIACQDVS
                     RNPWVKEVTMKKIYPNQVDISIVENKPGALLYSNDLYYLITAEGMILSAFSQFHNDFN
                     QYIITGLDIGLKKPGETVGDIAYQEVQRIIYVLNNIFPEQFYKIGIISKEEFLLFHKN
                     NNIKVRIESGEQLIREWYLLESALQKVTAEKMPSEEINMKYKERLLIILPD"
     gene            1014..2246
                     /gene="ftsA"
                     /locus_tag="PKJ95_03375"
     CDS             1014..2246
                     /gene="ftsA"
                     /locus_tag="PKJ95_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNR65320.1"
                     /translation="MKGEILLAGLDIGTNKTNTLIAELREDNTIDIVGVGSVPSQGVK
                     RGVIIDLDQAVESVRESVGNAERMAGVQIDSAVVAVNGSHISSFNSQGVIAISKEHPE
                     ITEEDIEKVIEAAKAGVIAPERDLIHVLPREFILDGQKGILDPLGMTGSRLEGKVHIV
                     TGSVTAIQNLLKCMEIAGIEVDELIFGNLACGNAILNRTEKELGVLLIDMGAGSSDIS
                     IFINDNIAYSSILPLGGIQVTNDLAIGLKISIEEAERLKIKYGNVMENYVSPDDVIEI
                     MRRNESTKESISKKYLIDIIEPRVHEIFSLIREEVERSNYLSTVTQGVVLTGGCALLP
                     GITDLASKVFERNVHIGRPNYQGELNDLVNEPRFSTVMGLLDYSMEQITATPSRRAKR
                     KKSGSTLQKAINWLRDFF"
     gene            2324..>3269
                     /gene="ftsZ"
                     /locus_tag="PKJ95_03380"
     CDS             2324..>3269
                     /gene="ftsZ"
                     /locus_tag="PKJ95_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNR65321.1"
                     /translation="MNIVPEKTGSFVDIKIIGVGGGGGNAVNRMIEEGINGVKHISTN
                     TDAQVLALSNAEQKIQIGARITMGMGSGSNPEIGKRAAEEDRDKISKALEGSDMVFIT
                     AGMGGGTGTGSSPVIAQISKELGALTVAVVTMPFSFEGIKRRKQAEEGIKLLKEIVDT
                     LIVIPNDRLLQIIAKETPILEAFKVADEILLHGIRGISEIVIEPGLINVDFADVKMIM
                     ENAGTAIMGIGRSTGENRAAEAAYNAVNSSLLGMKITDARGILFNISGGKNMTLYEVN
                     QAAEIIQEAANPDANIIFGAVINESLGEDIRITVIATGF"
BASE COUNT         1081 a          476 c          778 g          934 t
ORIGIN      
        1 gcgggagcga aaggattgat ggtgggggat gctcaggttt ccaccaaaca tgctaatttt
       61 atcttaaatc gaggacatgc tactgcctct gatatattgc gattgattga aaaaattgaa
      121 aaaatggtag aaaaaacctt tggtataaaa ctggagagag agattgattt tatcgggcta
      181 ccgggttaca gataattatg aaaacgaaga agagatttta gatgaggatg gggaagagga
      241 tagtagagaa tataatttat ttagagtatt gttattttac atcattgtag gatttttaag
      301 ctggaatatt ttctattttg tttttacttc ttcctggttt gacattaaaa gagtaaccat
      361 acatggcaat aattatttag atagtgatac tattttaact cagggtgaat tggacaaacc
      421 tgttaatatt tttcatttta acacagaaat agcctgccag gatgtttcca gaaatccctg
      481 ggtaaaagag gtcactatga aaaagatata tcccaatcag gtagatatca gcattgtaga
      541 gaataaaccg ggagctcttc tttacagcaa cgacctttat tatctgatta cagcagaagg
      601 gatgattctc tcagccttta gccagtttca taatgatttc aatcaataca ttataacagg
      661 cctggatatt gggttaaaaa aaccgggtga gacggttgga gatattgcct accaggaagt
      721 gcagagaata atctatgttc tgaataatat atttccggaa caattttata aaattgggat
      781 tatttccaaa gaagagtttc ttctatttca taaaaataat aatataaagg tcagaataga
      841 aagtggtgaa caattaatca gggaatggta tctgctggaa tcagctttac agaaggttac
      901 tgctgaaaag atgccatcag aggagattaa tatgaaatat aaggaaaggt tattgattat
      961 attaccggat tagaaggata ttttcttagg gcaatgcaaa taggaggatg tttatgaaag
     1021 gtgaaatttt gttagcaggt ttagatatcg gtacaaataa gaccaataca cttatagctg
     1081 aattaagaga agataatacc attgacattg ttggtgtagg ctctgtcccc tctcaagggg
     1141 ttaaaagagg tgtcattatt gatttagacc aggctgtaga gtcagttaga gaatctgtgg
     1201 gaaatgccga acgaatggca ggagtacaaa tagattctgc agtggtagct gtaaacggca
     1261 gtcacatttc ctcttttaac agtcaagggg taatagctat ttccaaagaa caccctgaga
     1321 taacagagga ggatattgaa aaagtaattg aggctgctaa ggcaggtgtt attgctccgg
     1381 agagggacct tatccatgtt ttaccacggg aatttatatt ggatgggcag aaaggtattt
     1441 tagaccctct gggcatgacc ggttctcgat tagaggggaa ggttcatata gttaccggct
     1501 cggtaaccgc tattcaaaat ttattaaaat gtatggagat tgccggtatt gaagtggatg
     1561 aattaatctt tggcaattta gcctgcggaa atgccatctt gaacaggacg gaaaaagaat
     1621 taggagtact cctcatcgat atgggagcgg gttcctcaga tatttctatc tttatcaacg
     1681 ataatattgc ctattcatct atacttcctc tgggcggaat tcaagttacc aatgacttgg
     1741 ctattggtct taaaatttcc attgaagagg cagaaaggct gaaaattaaa tatggcaatg
     1801 tgatggaaaa ctatgtttct cctgatgatg taattgaaat aatgagaagg aatgaatcga
     1861 caaaagaaag catttccaag aaatatctca tagatatcat tgaaccgaga gttcatgaaa
     1921 tattcagttt aatccgggaa gaggtagaaa gatcaaatta tcttagcact gttacacaag
     1981 gagtggtttt aacaggaggt tgtgcccttt tacctggaat tactgattta gcaagcaagg
     2041 tatttgaaag aaatgtacat ataggtagac cgaattatca gggtgaattg aatgatttgg
     2101 tcaatgagcc aagattttcg acggtaatgg gattgctcga ttattctatg gaacagataa
     2161 cggcaactcc ttccagaaga gcaaagagaa agaaaagcgg ttcaacgttg caaaaggcga
     2221 ttaattggtt acgggatttc ttttagatct gaaaaaaatt gatgtaaatt aaggaaaaat
     2281 atagataaag attagaccag gaattaggaa aggaggatta atcttgaata ttgtacctga
     2341 aaaaacagga tcgttcgtag atattaagat aattggtgta ggtggcgggg gtggaaatgc
     2401 tgtcaaccga atgattgagg aaggaataaa tggggtaaag catatttcta ccaataccga
     2461 cgcccaggta ttagctcttt ccaatgctga acagaagata caaataggtg ctcgtataac
     2521 tatgggtatg ggcagcggtt ccaaccctga aattggtaaa agggcggcag aagaagaccg
     2581 ggataaaatt agcaaggcct tagaaggttc agatatggtt tttattactg ccgggatggg
     2641 tggcggtaca ggcaccggga gtagtccggt tattgctcag atttccaaag agctgggagc
     2701 tttaacagtg gcggtagtta ctatgccttt ctcttttgaa ggtatcaaaa ggaggaaaca
     2761 ggcagaggaa ggaattaaat tgttaaaaga aatagtggat accctgatag tgatacctaa
     2821 cgaccgatta ttacagatta ttgccaagga aactccgatt ctggaggctt ttaaggttgc
     2881 cgatgaaata ttattgcacg gcatcagagg aatatcagag atagttattg aacccggatt
     2941 gatcaatgtt gacttcgccg atgtaaaaat gattatggaa aatgccggca ccgctattat
     3001 gggaattggt cggtccacag gtgaaaatcg tgctgctgag gcggcttaca atgcagttaa
     3061 tagttccctt ttaggaatga aaattactga tgcgcggggc atattattta atatttccgg
     3121 tggtaaaaat atgactctat acgaggtaaa tcaggctgcg gagataattc aggaagctgc
     3181 taatcccgat gccaatatca tcttcggggc agtgataaat gaatcactgg gagaggatat
     3241 caggataacc gttattgcta ccggttttg
//
LOCUS       DAOQWT010000130         3253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_31171_length_3253_cov_2.890244, whole genome shotgun sequence.
ACCESSION   DAOQWT010000130 DAOQWT010000000
VERSION     DAOQWT010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31171_length_3253_cov_2.890244"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..146
                     /locus_tag="PKJ95_03385"
     tRNA            71..146
                     /locus_tag="PKJ95_03385"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:104..106,aa:Gly,seq:ccc)
     gene            complement(180..398)
                     /locus_tag="PKJ95_03390"
     CDS             complement(180..398)
                     /locus_tag="PKJ95_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65322.1"
                     /translation="MAEQIVTLLILLFVIIVLSRFIARMMQSGNEKYRIPILYGRVLL
                     GFLLSMLIYLVFLMFKGENIITKLFGVS"
     gene            complement(403..1791)
                     /locus_tag="PKJ95_03395"
     CDS             complement(403..1791)
                     /locus_tag="PKJ95_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNR65323.1"
                     /translation="MSKKYDVIIVGAGPAGIFAALELLKNYNKDINILLLEKGKAIIN
                     RKCPMREQKTVCQQCNPCSLLSGWGGAGAFSDGKLNLTSDFGGNLTDYIAEEELNNLI
                     HYVDRLYCQFGAEDNLYGSNPKEIERVKREAAKANLKFIPARIRHLGTEQCFTVLKNI
                     YDFLKDKIEIRTDCQVDKIVKEGYEVCGVITSGGEYIKGENIILSPGRVGAEWLKNQA
                     IELELPVFRNPVDVGVRVEVPAVIFEHLTDTLYESKLIYYSSFFDDKVRTFCMCPHGE
                     VVMEYNNGITTVNGHSYRNKKTDNSNFAILVSTTFTKPFQEPIAYGKYIANLANILSG
                     GVIIQRLGDLILGRRSTSDRIAHSIVRPTLNSATPGDLSFVLPYRILTDILEMLEALD
                     KIAPGVYNEQTLLYGAEVKFYSARLDLSQQLETKIKNLFAIGDGAGITRGLIQASVSG
                     VIVAREIRDNRL"
     gene            complement(1951..3171)
                     /locus_tag="PKJ95_03400"
     CDS             complement(1951..3171)
                     /locus_tag="PKJ95_03400"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNR65324.1"
                     /translation="MLNKECLICTGCQKEYPLSRVFPRCDDCFEPLEVKICPSKIILK
                     KSISSPENIIVHYHSFYSFSETNQELSLQEGFTPLTKANHLSPLLDLDYLYIKNETVN
                     PTWSFKDRGTYTSLQHALSLGYKSIGTLSSGNMAVSVAAYGARAGIKTLILVSAELPP
                     EKLNPILIYNPILIRVLGDYGQIYYESLKIGQELGIYFLNSDIPFRVEGSKSIAFELC
                     QQLNYKVPDFVIIPTSSGGNARGIMKGFLEFYEQKLISKLPRIVAVQLEGCAPIYQAW
                     IGKQEKISAVKNPQAIDQAIVNPFPPSGNELLRKLKKYNGLVTAVSDYEALQGQRMLA
                     KEGIFAQPAAAVSIAAIRKLRKEGIINQKSRCVALVTGAGLKYSGVLEKQKFSFSTGK
                     LKDLKGIISTKFQD"
BASE COUNT         1002 a          696 c          548 g         1007 t
ORIGIN      
        1 gattgaaaat aaactgattt tcttatataa tattaactgc attgttacaa gcactgattt
       61 aaatatttga gcgggaatag ctcagtaggc agagcgttgg cttcccaagc cgaaggccgc
      121 gggttcaaac cccgtttccc gctccatttt tattataaat gatttcctaa gctataaaat
      181 tatgagactc cgaacagttt tgtaataata ttttctcctt taaacatcag gaaaactaaa
      241 tatatgagca ttgacaataa aaatcccagc aataccctgc cataaagaat gggtattcta
      301 tatttttcat tgcctgactg catcattctg gcaataaaac gggaaaggac aataattaca
      361 aacaatagaa ttaataatgt aacaatctgt tcagccatct gattataacc tgttatctct
      421 aatctctctg gcgacaataa caccactgac cgaagcctgt atcaaacctc tggttattcc
      481 tgctccatca ccgatagcaa agagattttt tatttttgtt tccagctgtt gggaaagatc
      541 caatcttgcg gaataaaatt taacctccgc accatagaga agagtctgct cgttataaac
      601 ccctggagca atcttatcca aagcttccaa catctctaaa atatcagtaa ggattcgata
      661 aggcagaaca aagcttagat ctcccggtgt agcgctgttt aaggttggtc tgacaattga
      721 atgggcgatg cgatcggagg tagacctcct tcctaaaatt aaatcaccca gacgttgaat
      781 aattacccct ccactaagaa tattagccaa attggcaata tattttccat aggcaatggg
      841 ttcctgaaaa ggtttggtga aggtagtact gaccagaatg gcaaaattgg aattgtcagt
      901 ctttttattc ctataactgt gaccattaac tgtggtaatg ccattgttat attccataac
      961 cacttcacca tgcgggcaca tacaaaaagt acgtacttta tcatcaaaga aagatgagta
     1021 gtaaattaat tttgattcat aaagggtatc agtaagatgt tcaaaaataa ccgcgggaac
     1081 ttctactctt actcccacat caacagggtt gcggaaaact ggtaattcta attcaatagc
     1141 ttgatttttc agccactctg cccccactct accgggagag agaatgatat tttcaccctt
     1201 tatatattct ccgcctgagg ttattacccc gcaaacctca tagccctctt ttactatctt
     1261 atccacctgg caatccgttc tgatctctat tttgtctttg agaaaatcat agatattttt
     1321 caaaacggtg aaacattgct cggttcctaa atgccttatc cgggcaggaa taaattttaa
     1381 atttgctttt gctgcctctc ttttaaccct ttcaatctct ttggggttac tgccatacag
     1441 gttgtcttct gctccaaact gacaatagag cctatcaaca tagtgaatca gattgtttaa
     1501 ttcttcttca gcaatataat cagtcagatt tccaccaaaa tcactggtga gatttaattt
     1561 tccatcacta aaagctccgg ctccacccca accggataac agagagcagg gattacattg
     1621 ttgacatact gttttttgct ctctcatagg acattttcta tttattatag ccttcccttt
     1681 ttctaaaaga agaatattga tatctttatt ataatttttc aataattcca gagcagcaaa
     1741 tatccctgcc ggtcctgcac cgacaataat aacatcatat tttttactca tttcattcac
     1801 cttattcccc aaacttcagc taacaataga taaccttcct taacaatctc ttaacgaaca
     1861 tatggttgaa aagcaaatat tatgccagat atcgctttat aacagcacta ttgtttcctg
     1921 atatggactt tccctaagct taagttaaaa ctaatcctgg aatttagtac taattatccc
     1981 ttttaaatct tttaatttac cggtagaaaa actgaatttc tgtttttcca aaactccact
     2041 atattttaat ccagctccgg ttaccagggc aacacaacgg gatttctgat ttattattcc
     2101 ctcttttctc aattttcgaa tagcggcaat agatacagct gctgcaggct gggcaaatat
     2161 cccctcttta gccagcattc gctgtccctg caatgcttcg tagtcagaaa cagcagttac
     2221 cagaccattg tactttttta attttcttaa aagttcattg ccactgggcg gaaaggggtt
     2281 gacaatagcc tggtcaatgg cctgaggatt ttttaccgca gagatctttt cctgtttacc
     2341 aatccaggcc tgatagatag gtgcacatcc ttctaattgg acagcaacaa ttcttggaag
     2401 tttagaaatt aatttctgtt cataaaactc taaaaaccct ttcataatac cacgggcatt
     2461 gccccctgaa ctggttggaa ttatcacaaa atccggtacc ttataattta actgctggca
     2521 taattcaaag gcaatactct ttgacccttc cacccgaaaa ggaatatctg aatttaagaa
     2581 atagattccc aattcctgac cgatttttaa actttcataa tagatttgac cataatcacc
     2641 cagtactcta attagaatag gattataaat caaaataggg tttaattttt ctggcggtag
     2701 ttcagcacta actaaaataa gagtctttat tccagcgcga gcgccatagg ctgctacaga
     2761 aactgccata ttgccactgg aaagggtacc gatagatttg taacccagac tgagtgcatg
     2821 ctgcaacgag gtataggttc ccctatcttt aaaggaccag gtagggttga cagtttcatt
     2881 cttaatatac aaataatcca gatccagtaa tggagagaga tggtttgctt tagtcagcgg
     2941 ggtaaaacct tcttgcaaag aaagctcctg gtttgtttct gaaaatgaat aaaaggaatg
     3001 gtaatgaaca ataatatttt cgggagagga aatcgatttt ttaaggataa tttttgatgg
     3061 acaaatctta acttccaatg gttcaaagca gtcatcacag cgagggaata ctctactgag
     3121 aggatattcc ttttggcaac cggtacaaat caagcactcc ttattcaaca tactcaccca
     3181 aattaatctg aaactctggt cggggcgaga ggatttgaac ctccgacccc ctgaacccca
     3241 ttcaggtgcg cta
//
LOCUS       DAOQWT010000131         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_31207_length_3250_cov_3.613146, whole genome shotgun sequence.
ACCESSION   DAOQWT010000131 DAOQWT010000000
VERSION     DAOQWT010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31207_length_3250_cov_3.613146"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="PKJ95_03405"
     CDS             complement(<1..1528)
                     /locus_tag="PKJ95_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65325.1"
                     /translation="MDKDMKILLSLEAKGRDKVFKDIDNTQKNIDNLRKKAIELTQAL
                     KEKTVAKHDTSEIEKNLKEVNKQLGEEKKLMGLLREEANKLIETEKTLQEEGKKASNA
                     LDSFAHIQNLQTIAASFSVISDSLSGVGNSLIDAAAKSETLKTAMYTAFQGAKDQADA
                     MMSWAKNFANVTPFETNEVVEATIKLKAYGVSASEIPAKLQQIGDMASGMGKGLNQAV
                     EAYADAQTGELERLKEFGITKAMIDKQMGGTLLNAQGQVKDLSKLMEGLSQIMEQRFA
                     GGMQKQSETFSGAVSNMRGEIQTLKETFGNLLLPTVKDVTIELTKFIKYVQDMPPELQ
                     KTVIAFGGMGTGLALIATGISGAISVLAPLGVVVNTLGTSIAGASALTLQSIPSIVGM
                     RTALLSYITTARTAIVTNATLGAGWVAALPIIAVGAMVIALGGLVYATQEYEKSVIQL
                     QKTDMETSLKKQANALNALKDVYPEATTSAIGFQKVIEEGIENILKQADGLFKYFLFP
                     L"
     gene            complement(1551..2081)
                     /locus_tag="PKJ95_03410"
     CDS             complement(1551..2081)
                     /locus_tag="PKJ95_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65326.1"
                     /translation="MDSEQQPLKIPYTSSLQKKKFNYDGLEVELKELPVLPFLAVIEA
                     FSNNDVEIFFNKLGFSEEERTLFYTQSMFEKLFKDFIDLHNENGEGNRKTKTLTLPEL
                     ISTIRRIVKIFAENFYSHPEEVGNKYSLRQMVLWLTSDKKKTGNDGFYVPPNAKNVKY
                     FKDEGGNPTVRYRIET"
     gene            complement(2056..2904)
                     /locus_tag="PKJ95_03415"
     CDS             complement(2056..2904)
                     /locus_tag="PKJ95_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65327.1"
                     /translation="MVQDVEKVNIGAGLVTYAPYNADYEDSDYPARQTIGMMKNVKLT
                     LNRTIQKIKDDVGGTMAIFKEIATEDGGTIEFVLGEDIPEERLMQLGVGTIEEVAAST
                     DAEVTRIVKLHKTGWQSLDIDYKTNPTIEFKIYTNVATPALIETVTITGGVTPVTGTN
                     VVIGQQEGTYLVRRIGTSVTIESDDTIQVVAEDVNVPGCRRLFMGGVNTVSTYRVWHR
                     KRNDDDTVNITTCKIGSAGKATIDNNPSESPGEDTFTADILVDTTKEAGKRLIQKDYG
                     LGTTAA"
     gene            complement(2907..3092)
                     /locus_tag="PKJ95_03420"
     CDS             complement(2907..3092)
                     /locus_tag="PKJ95_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65328.1"
                     /translation="MEVDVTGAIILISRKVIDNKKIRYSPGKTGEDEGFCLSAKAQGF
                     KSYILNDLQNHIMRRSE"
BASE COUNT          906 a          683 c          496 g         1165 t
ORIGIN      
        1 gaagagggaa tagaaaatat ttgaataacc catctgcctg ttttaaaata ttttctattc
       61 cctcttctat tactttctga aatccaatag cagacgtggt tgcttctggg taaacatcct
      121 tcaaggcatt taacgcatta gcttgtttct ttaaagaggt ttccatatct gttttttgaa
      181 gttgaataac agatttttca tattcctgag tagcataaac taatccaccg agagcgataa
      241 ccatagcacc cacagcaata ataggtaatg ccgcaaccca tcctgcccct aaagttgcat
      301 tagtcacaat agcagtccgg gctgtagtaa tataggaaag taatgctgtt ctcattccca
      361 caatagaagg aattgattga agggttaatg ccgatgcacc tgcaatagaa gttccaagtg
      421 tatttactac cactcccaaa ggggcaagaa ctgaaattgc acctgaaata ccagtggcta
      481 ttaatgcaag tcccgtaccc atcccaccaa aagcaataac ggttttttgt aattcagggg
      541 gcatatcttg aacatattta ataaattttg ttaattctat agtaacatct tttacagttg
      601 gcagaagaag atttccgaaa gtttccttta atgtttgaat ttcccctctc atattactta
      661 ctgcacctga aaaagtttca ctttgtttct gcataccacc ggcaaatctt tgttccataa
      721 tctgagataa accctccata agtttactca gatctttcac ctggccctgt gcatttaaaa
      781 gagttccacc catctgttta tcaatcatag ccttagtgat cccaaactcc ttcaagcgtt
      841 cgagttcccc ggtttgggca tctgcatatg cttccactgc ttgatttaat ccttttccca
      901 taccagaggc catatcacct atttgttgca attttgccgg tatctcagag gcagaaacac
      961 cataagcttt taattttatc gtggcctcta caacctcatt ggtttcaaaa ggtgtcacat
     1021 ttgcaaagtt tttcgcccat gacatcatag cgtcagcctg atcttttgca ccttgaaagg
     1081 ctgtgtacat agcagtttta agggtttcag actttgcggc ggcatctata agactattcc
     1141 ccacacctga taaactatct gaaataactg agaatgaggc ggctattgtt tgaaggtttt
     1201 gtatatgagc aaaagaatct aaggcattag aagccttttt cccttcttcc tgaagggtct
     1261 tttctgtttc tatgagttta ttagcctctt cccggagtaa tcccatgagt ttcttttctt
     1321 ctccgagttg tttatttact tcttttaaat tcttttctat ttcgctggta tcgtgctttg
     1381 caactgtttt ttcttttaat gcctgagtta attcaatagc tttttttctg agattatcta
     1441 tgtttttttg agtattatct atatctttaa aaactttatc ccgacccttt gcttcaagag
     1501 aaagtaatat cttcatatct ttatccattt atttttcacc gcctttctat ttaagtttca
     1561 atcctatatc ttacagtggg atttccaccc tcatctttaa aatatttaac atttttcgcg
     1621 ttaggtggaa catagaaccc atcattacca gttttctttt tatcactcgt aagccataaa
     1681 accatttgcc ttaaactata tttattaccc acttcttcag ggtgtgagta aaaattttcg
     1741 gcaaatattt taactattct tcttatggta gaaattaact cagggagagt aagggtttta
     1801 gtttttctgt taccctctcc gttttcatta tgtagatcaa taaagtcttt aaataatttc
     1861 tcaaacatac tttgagtata aaatagagtt ctctcctctt ctgaaaaacc gagcttatta
     1921 aaaaaaatct ctacatcatt attagaaaaa gcctctatga cggccaggaa aggaagaacc
     1981 ggaagttctt tcaactcaac ttccaggcca tcatagttaa actttttctt ctgtaaagaa
     2041 gaagtatatg gaattttaag cggctgttgt tccgagtcca taatccttct gtattaacct
     2101 ctttcctgct tcttttgtag tatcaaccag tatatctgcg gtaaatgtat cttctccagg
     2161 agattcagat ggattattat ctatagttgc tttaccggca gatcctattt tacaggttgt
     2221 gatatttaca gtatcatcat cattcctctt tctatgccat actctatatg ttgataccgt
     2281 attgactcct cccataaaga gccttctgca acctggaacg tttacatctt ctgctacaac
     2341 ctgaatagta tcatcacttt ctattgtaac tgaagttccg attcttctta caagataagt
     2401 tccttcctgt tgtccaataa caacattagt tcctgtaacg ggagtaactc caccggttat
     2461 agttactgtt tcaataagag caggagtagc cacattagta taaattttaa actctatagt
     2521 aggattggtt ttataatcaa tatctaatga ctgccatcca gttttatgta actttactat
     2581 acgggttact tcggcatcag tggaagcggc aacctcttca atagtaccaa ctccaagttg
     2641 cattaatctt tcctctggaa tatcttcacc cagaacaaat tcgatagttc ctccatcttc
     2701 tgtggctatt tctttaaata tagccattgt tccacctaca tcatctttta ttttctgaat
     2761 tgttcgattc aatgtcagtt tgacattttt catcattcct atggtttgcc tggcaggata
     2821 atccgagtcc tcataatctg cgttatatgg tgcatatgtt actaaacctg caccgatatt
     2881 tactttctcc acatcttgaa ccatacttat tcactccttc tcataatatg attttgtaaa
     2941 tcattaagta tataagattt aaagccttgt gcctttgcac ttaagcaaaa accttcatcc
     3001 tcacctgttt ttccaggaga ataacgaatt tttttattat caattacctt ccttgatata
     3061 aggattattg cccccgtaac atcaacttcc attatccctt taggtatttt cccttttggt
     3121 gtaaaattat aatttgaaac atcattacta actaacccgg ctattatatg ttttttctgc
     3181 tgaagtagat ttgaaagaat atcaggcttt ataataaatc agtggttcgt aattacatcc
     3241 tgatggtcga
//
LOCUS       DAOQWT010000132         3249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_31224_length_3249_cov_3.064809, whole genome shotgun sequence.
ACCESSION   DAOQWT010000132 DAOQWT010000000
VERSION     DAOQWT010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31224_length_3249_cov_3.064809"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="PKJ95_03425"
     CDS             <1..1036
                     /locus_tag="PKJ95_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR65329.1"
                     /translation="AIVYGLLQKLGGKVYVKSEPGKGSTFTLVLPRRIYDYHTSLPFE
                     MEDKEWEEIPEREAKESGLNQILLIEDNEQCIAQIKYLLEEEGFQVEAVRGGEQALSS
                     VKEKIPNGIILDLMMPGVDGFEVLRKLREDERTRAVPVLILTAKDLTEEEIAQLRYNK
                     IQQLVFKGAVNKKELVEKIKKMLKNGLGKSKSKEKSLKNIGNERNESTDERGVAAGNI
                     KKFNEKALILIAEDNPDNLLTMKAILKDHYKIITAVNGEEVLQKVYAEEPALILLDIG
                     LPKIDGFQILEKLKQDPKTKLIPIIAVTARGMSEEIEKILGQGCDDYILKPINVSNLT
                     EKIRYWLAKR"
     gene            1108..2163
                     /locus_tag="PKJ95_03430"
     CDS             1108..2163
                     /locus_tag="PKJ95_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR65330.1"
                     /translation="MSKILAIDDKKDNLIVLEAYLKQYLPSQQLIIAQSSKEGIEKAV
                     KERPDVILLDVSMPGMDGYQVCRELKKDEQTANIPIIMLTAIYTDTESMVKGLETGAD
                     AFIYKPFQPSELMSQIKVMLRIKKSDDLLRREKEVLEEMVAERTSALKDAQRAAVETI
                     ARILSIKDPQSSGNQYQIAKLSEAIGEKLRLSKIRLEGLWHASLINDIGKISIPADIL
                     NKHGKLTEIEYNFIKQHSQSAYEMLKDLPYPWPIAQIILQHHENIDGSGYPEGLKGEE
                     ILLEARILRVSDTVVAMLSHRPYRSRFNLEEVIEELKEKSNIYYDAGVTEVCIELLGD
                     DDFCKSNFLTTEDTPNP"
     gene            complement(2431..2700)
                     /locus_tag="PKJ95_03435"
     CDS             complement(2431..2700)
                     /locus_tag="PKJ95_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65331.1"
                     /translation="MDWKNISEIITNQGFPIALSIFLIFRIDRFMQELVSTQKEGYQQ
                     IFKQVSMVQKTLSKLNQQNRDYYTLFCTDIQKELASIKARFREAL"
     gene            complement(2723..3085)
                     /locus_tag="PKJ95_03440"
     CDS             complement(2723..3085)
                     /locus_tag="PKJ95_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HNR65332.1"
                     /translation="MFHKPSDIDYFERPSRRLTWSIVSLENKWAGADPSLRNKIGSPI
                     VITSGYRCITYNEEVRGVKGSYHLFGMAADIYAPQISLKELLQSAEEIGFSGIGYYPE
                     NNFLHLDIRPAGPARWQG"
BASE COUNT         1110 a          573 c          635 g          931 t
ORIGIN      
        1 agctattgtt tatggactgc tgcaaaaatt aggagggaaa gtttatgtaa aaagtgagcc
       61 gggcaagggc agtactttta cattagtact accgcgaagg atttatgatt atcacacctc
      121 tctgcctttt gaaatggaag ataaggaatg ggaggagata ccagagaggg aagcaaagga
      181 gtcaggttta aaccagattt tgttgataga ggataatgag caatgcattg cccagataaa
      241 atatttatta gaagaggaag gttttcaggt tgaagctgtc agaggagggg agcaggctct
      301 ctcgtctgtt aaagagaaga ttcccaatgg tattattctt gatctgatga tgcctggagt
      361 agatggtttt gaagtattga gaaaactcag agaagatgaa cgaacacgag ctgtaccggt
      421 attgattctt actgccaagg atttaactga agaagagatt gcacaactga ggtataacaa
      481 aatccaacaa ttggttttca aaggtgctgt taataaaaaa gaacttgtgg aaaagatcaa
      541 aaagatgtta aaaaatggtt taggaaaaag taaaagtaaa gaaaaatctt tgaaaaatat
      601 aggaaatgag cgaaatgaat ctacagatga aaggggtgtt gcagcaggca atattaaaaa
      661 atttaatgaa aaagccctca ttttaattgc cgaagataac cctgataatt tattaactat
      721 gaaggctatt ttgaaagacc attacaaaat tataacagct gtgaatggtg aagaagttct
      781 gcaaaaagtt tatgctgagg aacctgctct tattctttta gatattggat tacccaaaat
      841 agacggtttc caaatcctgg agaaactaaa gcaagatcct aaaacaaaat taattcccat
      901 catagcggtg acagcgaggg gaatgagcga agaaatagaa aaaatattag ggcagggatg
      961 tgatgattat attttaaaac cgatcaatgt ttccaattta actgaaaaaa tcagatattg
     1021 gctggctaaa agatagcact cttttctttg taatttgtat aaattataaa aatagttaca
     1081 aaaactctgg taaaggcagg gattcaaatg agtaaaattt tggcaattga tgataaaaaa
     1141 gacaatctta tagttttaga agcatattta aaacaatatt taccttcaca acagcttatt
     1201 atagcgcaat ccagcaaaga aggaatagag aaagcagtta aagagcgccc tgatgtcatt
     1261 ctattggatg taagtatgcc cggaatggat ggttatcagg tatgccggga attaaaaaag
     1321 gatgaacaga cggcgaacat tccaattatt atgttaaccg cgatttatac tgatacagag
     1381 agtatggtaa aggggctgga aacaggggca gatgcattta tctataaacc atttcaacca
     1441 tcagaattaa tgtcacagat taaggtaatg ttacgtatta aaaagtcaga tgatttgctg
     1501 agaagagaaa aagaagtttt agaggagatg gttgcagaac ggactagcgc tttaaaagat
     1561 gctcagcgtg ctgcagtaga aacaattgcc agaattttgt caatcaaaga ccctcaaagc
     1621 agtggaaatc agtatcagat tgctaagtta agcgaagcaa taggtgagaa actgagatta
     1681 tcaaagatac ggttggaggg tttatggcac gcttctttaa ttaatgatat tgggaaaata
     1741 agtattcctg cagacattct aaacaagcac ggtaagttga cagaaataga atacaatttt
     1801 atcaaacaac atagccaatc agcttacgag atgttaaaag acttacctta tccctggcca
     1861 attgcccaaa ttatcttaca gcatcacgaa aatatagatg gttcaggtta tcctgagggg
     1921 ttaaaagggg aagaaattct attagaagca cgtattttaa gagtttccga tactgtagtg
     1981 gcaatgctct cacataggcc ttatcgctcc cgtttcaatt tagaggaggt gatagaagag
     2041 ttaaaagaaa agagtaatat atactatgat gccggagtta ctgaggtctg cattgagctt
     2101 ttaggagacg atgatttttg taaatctaac ttcttaacta ctgaagacac acctaacccc
     2161 taatatttct tgccccccct gtttgacgca ttagtccctt acatttcttc ccacatcttt
     2221 attcactata taccaacgac catcgaccac ttccacttat ccccccatct ttttctttat
     2281 attttattaa aaactattaa ccatgaacta aaaaccgcct actcttatac tcacaatagt
     2341 ttacccataa acctctctca attattccca catctttatt cactatatac catctactaa
     2401 cgactactta catttacgct cacatcttat ctataaagct tctctaaaac gggctttaat
     2461 actggccagc tctttctgta tgtctgtaca aaacaaggta tagtaatcac gattttgctg
     2521 atttaatttg ctcaaggttt tttgtaccat agaaacctgt ttaaaaatct gctgataacc
     2581 ttctttttga gtagatacca gttcctgcat aaagcggtca atacggaaaa ttaaaaatat
     2641 ggacagggct atggggaaac cctgattagt aataatttct gaaatattct tccagtccat
     2701 ttttgcctcc ttttcctaaa aattagccct gccagcgagc tggcccggca gggcgtatat
     2761 ctaaatgtaa aaaattattt tcgggataat aaccaattcc cgaaaaacca atctcctccg
     2821 ctgattgtag gagttctttc aaagatatct gaggtgcata aatatcagca gccataccaa
     2881 acagatgata actgccttta actcctctca cctcctcatt gtaagtgata cagcggtatc
     2941 cagaggtaat aacaattggg cttccaattt tatttctcag acttgggtct gcccctgccc
     3001 atttattttc aagagatact attgaccatg ttaatcgcct tgatggtctt tcaaaataat
     3061 caatatcact cggtttgtga aacaaacatg cgccccaggc gaaatttgaa catattccat
     3121 ctgaatcccc taagcgatta atatgttata tattcccgta tgtatctccc ggaagccaga
     3181 gagggtataa cccatctccg tgccgtcgcg gatgccgtca ttatccgaat cgggatcaag
     3241 gcagttggg
//
LOCUS       DAOQWT010000133         3249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_31229_length_3249_cov_2.483406, whole genome shotgun sequence.
ACCESSION   DAOQWT010000133 DAOQWT010000000
VERSION     DAOQWT010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31229_length_3249_cov_2.483406"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKJ95_03445"
     CDS             complement(<1..436)
                     /locus_tag="PKJ95_03445"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNR65333.1"
                     /translation="MWENKLINEDGLTFDDVLLVPQNSKLIPKEVDLRTRLSRNISLN
                     IPLVSAAMDRVTESRLAIAMAREGGIGIIHKNMTIQEQVREIDRVKRSESGIIVEPIV
                     LTEDKKIKDALELMRHYHISGIPIVNYDKRLVGILTNRDIRFL"
     gene            complement(612..1376)
                     /locus_tag="PKJ95_03450"
     CDS             complement(612..1376)
                     /locus_tag="PKJ95_03450"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNR65334.1"
                     /translation="MLLVLDAGNTHTVIGVFDGDKLKNHWRIATDLRKTEDEFAMLIK
                     NLLADEALSFADIKAVIISSVVPPLIWILKKMFREYCQAEPLLVNSKIKLNIKIKTDY
                     PEEVGADRIVNAAAVHTLYGSPAIIIDFGTATTFCALDREGNYIGGAIAPGLELSSNA
                     LFEKTSKLPKVELKKCEYAICRNTVQAVQSGVYFGHLGLTRELIERFKQELKGHPKVI
                     ATGGYAELMSKDASLFDRVDLLLTLKGLKIIHDLNT"
     gene            complement(1409..2749)
                     /locus_tag="PKJ95_03455"
     CDS             complement(1409..2749)
                     /locus_tag="PKJ95_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNR65335.1"
                     /translation="MNKIMVVDDEQSIRIMLRRVLSDWQYEVNEAKNGTEALNKMNKE
                     KYTAILLDLRMPEMNGLQVIEKMVEMDINTPVIMMSAYGTVPEAVEAIKLGAMDYLVK
                     PFDLDELKMTLERIIRQDEIKNENQYFREEEERRFNFKEIVGQSSAMKRVLEMVKKVA
                     PLPTTVLITGESGTGKELIARALHQNSPRREKPFVVANCVAFSPSILESELFGHEKGA
                     FTGADSRRIGRFEIAHGGTLFLDEIGEINIATQAKLLRVVQEKDFERVGSSTSIKVEV
                     RLIAATNKDLEKEVKEGRFREDLFYRLNVFHIDIPPLRERKEDIPRLVQHFITKYNQV
                     LNKRITAISSEGVSLLMDYSFPGNVRELENIIERATIMCNSNTIEKELLFFLEQKNAS
                     PKAGTLKEMEREMIKKTLLQNNGNRTRTAQHLGMSRRNLILKLKEYQIVIKPER"
     gene            complement(2742..>3249)
                     /locus_tag="PKJ95_03460"
     CDS             complement(2742..>3249)
                     /locus_tag="PKJ95_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR65336.1"
                     /translation="QVLDFSRPKKLNITCFALGDMISELVNLLKLERREKEIQFELSL
                     ECSRQNIFADKDQIRQALMNIILNSIQAVPPGGVIDISLQLTTYQNEEALLISIKDNG
                     AGISQEELSHIFDPFFTTRSQGTGLGLSIAHNIIEMHQGTIVVESEKGKGTEVKIFIP
                     AGGSREIE"
BASE COUNT          894 a          747 c          518 g         1090 t
ORIGIN      
        1 ctaaaaagcg aatatccctg ttggtcagta taccaactaa tcttttatca taatttacta
       61 tgggaattcc ggagatatga taatgtctca tcaactccag ggcatcttta atttttttgt
      121 cttcagttaa aactatgggc tccacaatta ttccactctc agagcgcttt accctatcaa
      181 tctcccgtac ctgttcctga atggtcatat ttttgtgaat tatccctatt cctccttccc
      241 gggccatcgc tattgccagt cttgattcgg ttaccctatc catagcagcg ctcaccagag
      301 gaatgttaag acttatattt ctggataggc gggtacgtaa atcaacttct tttggaatta
      361 atttagaatt ttgaggaacc agtaagacat catcaaaggt cagtccgtct tcatttatta
      421 acttattttc ccacataaaa accacctctt tcccttcccc cttattaaac tcttaaatat
      481 aaatattaaa tataattctt ttctaaaagg gcgataaaac tttaaacaat cttatctgtt
      541 cttaaattag atgtcaaact aaaaatattc ggatttttta atactttcag acaattatcc
      601 ggctatactc attaagtgtt taaatcgtgt attattttca accctttcag ggtaagcaaa
      661 agatctaccc tatcaaataa tgaggcatct ttgctcatca gttcagcata ccctccggtg
      721 gcaataactt tgggatgccc ctttaattcc tgtttaaagc gttcaatcaa ttctctggtc
      781 aatcccagat gaccaaaata gacaccggat tgaacagctt gtactgtatt gcggcagata
      841 gcatattcgc atttttttaa ttccacttta ggtagtttgg aagttttctc aaataaggca
      901 ttgctggaca attccagacc aggagctatt gccccgccaa tatagttacc ctctctatct
      961 aaggcacaga aagtagtagc agtcccaaaa tcaataataa tagccgggct tccataaagg
     1021 gtatggactg ctgcagcatt tactatccta tcggcaccaa cctcttccgg ataatcggtc
     1081 tttattttga tattcagttt aattttggaa ttaaccagca atggttctgc ctgacagtat
     1141 tcacggaaca tcttctttaa gatccatatt aagggaggaa caacgcttga tattatcact
     1201 gccttaatat cagcaaagga gagcgcctca tcagccaata aatttttaat aagcatagca
     1261 aattcatctt cagttttcct cagatcagtg gctatgcgcc agtgattttt taatttatcc
     1321 ccatcgaata ccccaattac ggtatgagta tttcccgcat ctaataccag taacattgta
     1381 tctttacctc ccatatcata gtattccttt atctctccgg tttaataact atctgatatt
     1441 ccttcaattt taaaattaga tttcttctgc tcatccctaa gtgttgcgct gttctggttc
     1501 tgttaccatt attttgaaga agagtcttct taatcatctc tctttccatc tctttcaggg
     1561 tgccggcttt agggctggca tttttttgct ctaaaaagaa gagtaattct ttttctatgg
     1621 tattactatt acacattatt gttgctcttt caataatgtt ttccagttct ctcacatttc
     1681 cggggaaaga atagtccatt aataaggata ccccttcaga ggaaatcgcg gtaatccgtt
     1741 tatttaaaac ttgattatat tttgttataa aatgctgtac cagccgagga atatcctctt
     1801 ttctctctct taaaggagga atgtcaatat gaaagacatt aaggcgataa aagagatctt
     1861 ccctgaacct accctcttta acctcttttt ccaaatcctt attggtggca gctatcagcc
     1921 ggacttcaac ctttatactg gtagaactac ccactctctc gaaatccttt tcctgtacca
     1981 ctctcaacaa ttttgcctgg gtagcaatat ttatttctcc tatttcatca agaaagagag
     2041 tcccgccatg ggcaatctca aaacgaccta ttcttctgga atctgctccg gtaaaagcac
     2101 ccttctcatg gccaaatagc tcgctctcca ggatactggg agaaaatgcc acacagttag
     2161 ccaccacaaa aggtttttcc cggcggggac tgttctgatg aagggcacgg gcgataagct
     2221 ctttcccggt gccgctctcc ccggtaatca atactgtggt gggaagagga gcaacctttt
     2281 ttaccatttc cagtaccctc ttcatagctg aactctgtcc gacaatctct ttaaaattaa
     2341 atcttctctc ttcttcttcc ctgaaatatt gattttcatt ctttatctca tcctgacgga
     2401 taatacgttc taaggtcatc tttaattcat ccagatcaaa gggtttgacc agataatcca
     2461 tcgctcccaa ttttattgcc tctactgcct cgggaactgt tccataggca gacatcataa
     2521 ttacaggtgt gtttatatcc atctctacca ttttttcaat aacttgaagc ccgttcattt
     2581 ctggcattct caaatccaga agtatggcag tatatttttc tttattcatc ttgttcagcg
     2641 cctcagtacc atttttcgct tcattaacct catactgcca gtcagaaagt actcttctga
     2701 gcattattct gatactctgt tcatcgtcaa caaccataat tttattcaat ctctctactc
     2761 cctcctgcgg ggataaatat ttttacttca gttcccttcc ctttctctga ttctactaca
     2821 atggtaccct gatgcatctc gataatatta tgagcaatgg ataaacccaa tccggttccc
     2881 tggcttcggg tggtaaaaaa gggatcaaat atatgtgaaa gctcctcctg cgatatccca
     2941 gcaccattat ctttaatgga aattaagaga gcttcctcat tctggtaagt ggttaattgt
     3001 aaagaaatgt caattactcc tcccgggggg accgcttgaa tggaattcaa gataatattc
     3061 attaaggcct gtcttatctg gtctttatcg gcaaagatat tctgccggga acactccagg
     3121 gaaagctcaa actggatttc tttttctctt ctctctaatt tcaagagatt taccaattct
     3181 gaaatcatat cacctaatgc aaagcaggtg atatttaact ttttcggtct ggaaaaatcc
     3241 agcacctgg
//
LOCUS       DAOQWT010000134         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_31327_length_3241_cov_3.628060, whole genome shotgun sequence.
ACCESSION   DAOQWT010000134 DAOQWT010000000
VERSION     DAOQWT010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31327_length_3241_cov_3.628060"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKJ95_03465"
     CDS             <1..294
                     /locus_tag="PKJ95_03465"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNR65337.1"
                     /translation="YGCSTRPALAAMKKARKKGIKAGVLQLQTLWPFPEAIVKSAVSQ
                     AKLILVPELSLGQLKSEITKYCPKKTVIGINKLGGTTIQPDDILAFLSKEIEL"
     gene            291..1103
                     /locus_tag="PKJ95_03470"
     CDS             291..1103
                     /locus_tag="PKJ95_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNR65338.1"
                     /translation="MKYSGKKYVKEELLPFFWCSGCGNGIIFAALIRAMEENNLEKKD
                     IVAVTGIGCWGKADDYLKTHAFHGTHGRAIAVATGIKLANPLLHIFALVGDGDGATIG
                     GNHLIHAARRNIDIITIMSNNLNYGMTGGQYSGTTPFDSITSTSRRGHAEDAFDISNL
                     VKSAGAGYVARCTIDNPRMIQRFISKAIKKERGFRFIEVINICPTYFGRYNKFNNAVE
                     MIKDIKERTVNIKDIEREGQEDLDGKIVVGEYIDKKVEGFLERYWGKIRNNA"
     gene            1157..1702
                     /locus_tag="PKJ95_03475"
     CDS             1157..1702
                     /locus_tag="PKJ95_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNR65339.1"
                     /translation="MIYQEKDRFEIIIGGVGGQGNVSCGILIGEAASCYEDKFVTMTY
                     SYGTEARGTFARTDVLVGNQFIDFYECQRPDVILILHDKAYPKIKGKIFKDTIVVINQ
                     NEVKDVDMDLGKHFSLPLSDIAMKIGSLQVTNTIALAFIVNVTKFIDKTSLVKAVQSE
                     YANGKIARLNEKALEYGFNLH"
     gene            2059..>3241
                     /locus_tag="PKJ95_03480"
     CDS             2059..>3241
                     /locus_tag="PKJ95_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR65340.1"
                     /translation="MNFIKKISIIILTTLLVFTLVNGAAFAQEKIIKIGTLFPLTGSC
                     ALAGSRCKAAVETAVEVINNSHPECDVPLAAQEGLLGGYKIVLVHADHQGKPDIAKAE
                     AERLFDQEGVYTIVGCYNSACTNPASMVAERQEKIFMCGCSSSAALTERGLQYFFRMA
                     PTDKTESIEFCDVIEWMNGTKGANLKTIGIIYENTEFGKHAADEGKLAAAEAGFEVVV
                     DVPFSPGATNMNSEVQTLKAKNPDVVFGACLGGDYTLWVRTMKQMDWLPKMCLNYCTG
                     YQDPVITEQLGDDANYFMGGTGYAPEFAELMPAVAAVEKIYQEKIGVPFDSDSIQEAV
                     ATFILAQAIEKAGTLNYDTVRDTLYANEWNSPLSMGGKVAFVSGGQNAKAMSMITQLQ
                     GG"
BASE COUNT         1076 a          505 c          699 g          961 t
ORIGIN      
        1 tatggctgca gtacccgtcc tgctcttgct gccatgaaaa aagccagaaa aaaaggtata
       61 aaagcaggag ttttacaatt acagaccttg tggccctttc cggaggcaat agtcaaatca
      121 gctgtttctc aggcaaaact tattctggta ccagaattat ctctgggtca attaaaatca
      181 gaaataacca aatactgtcc aaaaaaaaca gtcattggta ttaataagct gggaggaaca
      241 actatccaac cagatgatat actggctttt ctttccaagg agatagagtt atgaaataca
      301 gtggtaaaaa atatgtcaaa gaagagttgt tacctttttt ttggtgctca ggctgtggga
      361 acggtattat ctttgctgct ctgattagag ccatggaaga gaataattta gaaaaaaaag
      421 atatagtagc cgttaccgga attggctgtt gggggaaagc tgacgattat ttaaagacac
      481 atgcctttca cggaacacat ggaagagcaa ttgcggtagc aacaggaatc aaattggcca
      541 atcccctatt acatatattt gcgttggtgg gagatggtga tggtgctacc ataggaggca
      601 accatttaat acatgcagcc cgtcgaaata ttgatattat aacaattatg tctaataatc
      661 tgaattatgg catgaccgga ggacagtatt ctggaaccac accatttgat tcaatcacct
      721 ccacctccag aaggggacat gcagaagatg cttttgatat atccaactta gttaaaagtg
      781 caggtgctgg gtatgttgcc agatgtacta tagataaccc gcgaatgatt cagcgcttta
      841 tcagtaaagc tatcaaaaaa gagagagggt ttcgttttat tgaagtaata aatatctgcc
      901 caacctattt cggaagatat aacaagttta ataatgctgt tgaaatgatt aaagacataa
      961 aagaacgaac agttaatata aaagatattg agagagaagg ccaggaagat ttggatggaa
     1021 aaatagtggt tggtgaatat attgataaaa aagttgaagg gtttttagaa cgttattggg
     1081 gaaaaataag aaataatgca taagtttatt tttaacccga atagtgaaga acctgttaag
     1141 aataaggagc actattatga tataccaaga aaaagataga tttgaaataa ttattggcgg
     1201 agttggtgga cagggaaatg tctcctgtgg tattcttatt ggagaagcag ccagttgtta
     1261 tgaagataaa tttgttacca tgacctattc ttatggtact gaggctagag gtacctttgc
     1321 caggactgat gtgttggttg gaaaccaatt tattgatttt tatgaatgtc aaagacccga
     1381 tgtaatatta atattacatg ataaggcata tccaaaaata aaaggaaaaa tatttaaaga
     1441 taccatagta gttattaatc aaaatgaagt aaaagatgta gatatggatc tgggaaaaca
     1501 tttttcttta cccctttctg atatagctat gaaaataggc tctttacaag ttactaacac
     1561 cattgctttg gcttttattg tgaacgtcac aaaattcata gacaaaacat ctctggtaaa
     1621 ggctgtacaa agtgagtatg ccaacggtaa aattgccagg ttaaacgaaa aagccttgga
     1681 atatggtttt aatctgcatt aaatatatta gaaaatgaaa ttttattttt ctgttgagat
     1741 aaaaatgtga acttttttaa aacctgctct gcgtaactag tttctcttta ttaaggcaat
     1801 tggtaaataa agaatatatt tctaatatgt tcaatttttt agaaaaaatg tttatagatt
     1861 tgcctgaagg gaaaaattat gccagatatc tctcacacat atatcttact tttttcatca
     1921 taatacccaa taattattac tcatatcact tgatcttgta aataagatta taaggaggtg
     1981 ttaagaaaag aagaagaaaa agattaactc ttatttattt taaaaatata ttttaaacag
     2041 ttaaggagga gaatataagt gaattttata aaaaaaatct caattatcat tttaacaact
     2101 cttttagtat ttactttggt aaacggtgcc gcctttgccc aggaaaagat aattaaaatt
     2161 gggacactct ttccattgac cggttcctgt gctttagcgg gaagccggtg taaagctgct
     2221 gtagaaactg cagtagaggt gattaataat tcacatccgg aatgtgatgt tcctctggca
     2281 gcccaggaag ggcttcttgg tggatacaaa attgttttag tacatgctga tcatcaggga
     2341 aagcctgata ttgcaaaagc tgaggctgaa aggttgtttg accaggaggg tgtatatact
     2401 attgttggtt gttacaatag tgcctgtacc aatcctgcca gcatggtagc agagagacag
     2461 gagaaaatat ttatgtgcgg ttgctccagt tcagctgctt taacagaacg tggtttacaa
     2521 tatttcttta gaatggcacc aactgacaag actgaatcca tcgaattctg tgatgttatt
     2581 gaatggatga atggaaccaa aggagctaat ttaaagacca ttggtatcat ctatgagaat
     2641 actgaatttg gcaaacatgc cgctgatgaa ggtaaattag ctgcagccga agctggattt
     2701 gaagtagtgg tagatgtacc cttcagcccc ggtgctacca atatgaatag tgaagttcaa
     2761 actctgaaag cgaagaatcc ggatgtagta ttcggtgcct gcctgggtgg agactacact
     2821 ctctgggtaa gaacgatgaa acaaatggac tggctcccca aaatgtgctt gaactattgt
     2881 accggatatc aggacccggt tattactgaa caacttggtg atgatgccaa ttactttatg
     2941 ggcggaacag gttatgcccc ggaatttgcc gagctgatgc ctgcagttgc tgcagtagaa
     3001 aagatttatc aggagaagat tggtgttccc tttgatagtg attcaattca agaggcagta
     3061 gctaccttta ttttggctca ggcaattgag aaggctggta cccttaatta tgatactgtt
     3121 cgtgataccc tgtatgccaa tgaatggaat tctcctctgt ctatgggtgg caaagtggct
     3181 tttgtttccg gtggacagaa tgctaaagcc atgagtatga taactcagct gcaaggcgga
     3241 g
//
LOCUS       DAOQWT010000135         3234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_31418_length_3234_cov_3.341931, whole genome shotgun sequence.
ACCESSION   DAOQWT010000135 DAOQWT010000000
VERSION     DAOQWT010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31418_length_3234_cov_3.341931"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="PKJ95_03485"
     CDS             <1..1877
                     /locus_tag="PKJ95_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR65341.1"
                     /translation="LDISALVAGAKYRGEFEDRLKAVLKEVENAAGSIILFIDEMHVL
                     VGAGAAEGAIDASNMLKPALARGELHAIGATTLKEYRKYIEKDAALERRFQMVLVKEP
                     TVEDTIAILRGLKERYEVHHGVKIMDSALIAAATLSDRYITDRFLPDKAIDLIDEAAS
                     GLRMEIDSMPIEIDEIQRKIMQLQIEKQALKKENNAISREKIKNIDNNLGNLEEEKNN
                     LMLRWNNEKEIIQKINQIKSEIEKVKTEAEQAERNGDLNRLAEIKYGKMPELEKQLEE
                     NNEKLSSLQKSSRMLKEEITEEDIAQIVAKWTNIPVGRLMEGETAKLINIEQELKKRV
                     VGQDEAVNLVANAIRRSRVGISDPNRPVGSFIFMGPTGVGKTELARSLAAFLFNDENA
                     LVRIDMSEYMEKHSVARLIGAPPGYVGYEEGGQLTEIVRRRPYSVILFDEIEKAHHDV
                     FNILLQIMDDGRLTDGQGRVVNFKNTILIMTSNIASQYIYQLNSSNKEEMSRQIEQVL
                     REHFKPEFLNRVDEIITFNRLGKEQIIEIVDIQLNILRKRLQGMEIDLIVSNRVKEIL
                     AERGFDPQFGARPLKRTIQRQLQDPLALQILEGKIKEKDKVQVEFEEKKKEIIFKIKS
                     "
     gene            complement(2048..3106)
                     /locus_tag="PKJ95_03490"
     CDS             complement(2048..3106)
                     /locus_tag="PKJ95_03490"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNR65342.1"
                     /translation="MSLTRFLKGFLIILIGVILLLNNLNILEWTVWPNILKLWPLLLI
                     SLGISLIFRKRLSWLAPLVILLGIIYGISASYTDLNLQLEGKIPIEVETIQREVEMVP
                     AAIPKIEEEVISETIPEEEVPEKTIEAEDIEDEKLLVSDTLGDKETTKEFEMVPLVQK
                     ANLHLNYTVGSFVLRFPTPLVYQCQVSYRYPGFKPLEDFSVQDHEAKILITHQSVTDQ
                     MVRNSQNHIDLQLNTGIVYDIFLETGATSIDYDLSKFKIENFSIKSGASKIDIIAPQY
                     NGKININSGVSKIDIAIPDNVGAKVILDTGISKKDFDEHFSKQDGNVYFSENYNNAEY
                     RVDIKIDSGISKIHIYYL"
BASE COUNT         1096 a          481 c          654 g         1003 t
ORIGIN      
        1 ctctggatat aagtgccctg gtagccgggg caaaatatcg tggagagttt gaagatcgtc
       61 ttaaagcggt attgaaagag gtagaaaatg ccgctggttc tataatttta tttattgatg
      121 agatgcatgt tctggttggt gccggggcag cagagggggc tattgatgct tctaatatgt
      181 taaaacctgc tttagccaga ggagaattgc acgccatagg cgctaccact ttaaaagaat
      241 atcgtaaata tattgagaag gatgcagcct tagagagaag atttcagatg gtcctggtta
      301 aggaacctac tgtagaggat actattgcta ttctacgagg tttaaaagaa cgttatgaag
      361 tgcatcatgg ggtaaaaatt atggattccg ccctaattgc tgctgcaaca ttatctgatc
      421 gttatattac cgatcggttt ttaccggaca aagctattga tttgattgat gaggctgcct
      481 caggactgag aatggaaatt gatagtatgc ctatcgagat agatgaaata cagagaaaga
      541 taatgcagct acagatagaa aaacaggctt taaaaaagga aaataatgcc atttccagag
      601 aaaaaataaa aaatattgat aataatctgg gtaatctgga agaagaaaaa aataatttga
      661 tgttaagatg gaacaatgaa aaagagatta ttcagaagat caatcagatt aaaagtgaaa
      721 ttgaaaaagt taaaactgag gcagaacaag cagaaaggaa tggtgacctt aaccgattag
      781 ctgaaataaa atatggtaaa atgccggaac tggagaaaca gctggaagaa aataatgaaa
      841 aattatcttc cttgcagaag agttcccgta tgctaaaaga agagattacc gaagaagata
      901 ttgctcagat tgtggctaaa tggaccaaca tccctgtagg gcgtttaatg gaaggggaaa
      961 cagcaaagct gataaatatt gaacaggaat taaagaaaag agtagtgggg caggatgagg
     1021 cagtgaatct ggtagctaat gctataagac ggtccagggt tggtatctca gatcccaata
     1081 gaccggtagg ttcatttatt tttatgggtc ctaccggagt tggaaagact gaattagccc
     1141 gaagcttagc agctttttta ttcaatgatg agaatgcact ggtcagaata gatatgtcgg
     1201 aatatatgga gaaacacagt gttgccagat taattggtgc tcctcccgga tatgttggtt
     1261 atgaggaagg aggtcaatta accgaaattg tcaggcgcag accctattca gtaattcttt
     1321 ttgatgaaat tgaaaaagct caccatgatg tttttaatat tttgttacaa ataatggatg
     1381 atggaaggtt gacagatgga cagggcaggg tggttaattt taagaatact attcttatta
     1441 tgacctccaa tattgctagt cagtatatct accaattaaa ttctagcaat aaagaggaga
     1501 tgtccagaca gattgaacag gtattgagag aacattttaa acctgagttt ttaaacaggg
     1561 ttgatgagat tataaccttt aatcgtctgg gaaaagaaca gattattgag attgtagata
     1621 tccagctcaa tattttgaga aagcggttgc agggtatgga aattgactta atagtgagta
     1681 acagagttaa agaaattctt gctgaacgcg ggtttgaccc tcagtttggt gccagacctc
     1741 taaaaagaac tatccagaga cagttgcaag atcctttggc tttacaaatc ctggaaggga
     1801 agataaaaga aaaggataag gtccaggtag aatttgagga aaagaaaaaa gaaataattt
     1861 ttaaaataaa aagctaagtt tttaatatta tttaaatgcc cctcttaaat tggggcattt
     1921 ttttattttt tttggcaaat taatttgtat ttgtaacact attgatgaaa ttaaatagac
     1981 ctaagcattg taaaataata cataattatt atttttattt tgtttgtcta atgtaataaa
     2041 tcttaaatta taaataataa atatggattt tggaaatgcc agaatctatt tttatatcaa
     2101 cacgatattc agcattatta taattttcag aaaaataaac attgccatcc tgttttgaga
     2161 aatgctcatc gaaatctttt ttactgattc ctgtgtctaa tattaccttt gctcctacat
     2221 tatcaggaat tgcaatatca atcttagata ctccggagtt tatgttgatt ttcccattgt
     2281 attgtggtgc tataatgtca atttttgagg cgccgctttt tatggaaaaa ttttctattt
     2341 tgaattttga taaatcataa tcaatagaag tagcaccggt ttcgagaaaa atatcgtata
     2401 ctataccggt atttagttgt aaatcgatat ggttttggga attccttacc atttgatcgg
     2461 tgactgactg atgggtaatt aaaatctttg cttcatggtc ctgaacagaa aaatcttcaa
     2521 ggggcttaaa ccctggatag cgataactca cttggcattg atagaccagt ggggtaggga
     2581 atcttaggac aaaagaaccg acagtataat ttaaatggag attagccttt tgaaccaaag
     2641 gaaccatctc aaattcttta gttgtttctt tgtctcctag agtgtcagat actaacaatt
     2701 tctcatcttc tatgtcttcg gcttcaatag tcttctctgg cacctcttct tcagggattg
     2761 tctctgaaat aacctcttct tcaattttgg gaattgctgc tggaaccatc tccacttctc
     2821 tttgtatagt ttctacttca atcggtattt taccttctaa ttgcaggttt aaatcggtat
     2881 aactggcgct aataccataa ataattccca ataagatgac cagaggagct aaccaggaga
     2941 gcctcttcct gaaaattaaa ctaatgccta gacttatcaa caacaatggc caaagcttta
     3001 aaatattggg ccagacagtc cattccagaa tattgagatt gtttaataat aagataacac
     3061 caataaggat aatgagaaat ccttttaaaa accgtgttaa actcattttt agaactcctc
     3121 tctaaaattt ggagatattg aaaattctat catatatttt tacccacatc gtacttgtaa
     3181 ggtaattata ttactttccc atatattagc tgaattaatt atttttgagt gata
//
LOCUS       DAOQWT010000136         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_31641_length_3218_cov_2.828644, whole genome shotgun sequence.
ACCESSION   DAOQWT010000136 DAOQWT010000000
VERSION     DAOQWT010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31641_length_3218_cov_2.828644"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..957
                     /locus_tag="PKJ95_03495"
     CDS             166..957
                     /locus_tag="PKJ95_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNR65343.1"
                     /translation="MERKVDKEYKALLLAGGKSKHALKKMTGQEYKALMTIDTLDSGP
                     VISHIIEELKKSRYISQIYVSGPNEVQRKITSGELMVLPAGNTLMETLQKNISLFQNE
                     PYILIISCDIPLVRSKHLENFLEDCQANPGFDIYYAIINKEDYLKQFPQNELRRIYAN
                     LLEGSYTGGNVFFINPKVIVDCADMIEQFILFRKHPLKMANILGRRVVVKYLKKYLSI
                     RDLERMVPEYLKGYTGKAIPAAPEIALDIDKPVQLEVLKELTRKQ"
     gene            complement(961..1515)
                     /locus_tag="PKJ95_03500"
     CDS             complement(961..1515)
                     /locus_tag="PKJ95_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HNR65344.1"
                     /translation="MNDLLNNMHNLNPYIGALLIFFFRIIDVSLGTYRIQMIVSRKKL
                     LAGIIGFVEVFIFILIVSKVIHDVGNWMNILGYCGGFATGTIVGIMITEKTSHEIISV
                     EVISRKHWPEIEKRLREEGFGVTRNLGYGLQGELQVLRIITQRSLFPKARDIALEYDP
                     KAFITSNLIVGRSGGYIYGVKGKL"
     gene            1991..>3218
                     /locus_tag="PKJ95_03505"
     CDS             1991..>3218
                     /locus_tag="PKJ95_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNR65345.1"
                     /translation="MNAEIISIGNEILGGNIVDTNPNYIVKKLKEINLNVQHISAVGD
                     EEKEIITQLKEAYQRSQIIITTGGLGPTEDDITFQSVARTFQLNLVKYPEAERHMVER
                     ISKIGVFVSPSNLKQSFLPEGSTCIINPYGTAPAMILEKEDRVIISLPGVPLEMKNLL
                     SEQIIPFLQKKFPNLEHLQSRIIRTSGLGESNLNDMIKDFILSNQRLNVGIYASPEDI
                     QIQLNALSKTPEETKNILDKATEQLKVLLGDYIFGYNDESLEGVIGNLLKRKNLTLAV
                     AESCTGGMLGGAITAVPGSSDYFKGGIISYDGEIKENLLGVPRSILINFGQVSEPTAR
                     AMAEGVRKMCNSDLSLSITGIAGPAGGSPEKPVGLVYIALADNEQVLVQKHQFHNDRQ
                     AIRLRSVRRALNMLRLY"
BASE COUNT         1127 a          572 c          566 g          953 t
ORIGIN      
        1 tcctatctct ataggaaaga acggcaatgt gtaaacacat cttaagagta atacaaataa
       61 tataataata attaaaacct actgattaaa accataaacc ttctaccagt aatagacctt
      121 tttctactaa ctattatata atagaataag aaaaacggaa aatatgtgga aagaaaagtg
      181 gataaagaat ataaggctct tttactggct ggaggaaaaa gcaagcatgc cctaaaaaaa
      241 atgaccggac aggaatacaa agcactaatg accattgata cccttgattc aggccctgta
      301 atatcccata tcattgagga attaaaaaaa tccagatata tttcacaaat atatgtttcc
      361 ggtccaaatg aagttcaaag aaagattaca tccggggagc ttatggtact tcctgccggt
      421 aatactttaa tggagacatt gcaaaaaaat atttctctct tccaaaatga accatatatt
      481 ttgataatct cctgtgatat ccccctagtt agaagcaaac atctggaaaa ttttttagaa
      541 gactgccagg ctaatccggg ctttgatatc tattatgcca tcatcaacaa agaagattat
      601 ttaaaacaat tcccccagaa tgaattaaga agaatttatg caaatctctt ggaaggatcc
      661 tacacaggag gtaatgtatt tttcataaat cccaaagtaa tagtggattg tgccgatatg
      721 atagaacaat tcattctttt ccgaaaacat ccccttaaga tggctaatat tttagggaga
      781 agagtagtag taaaatatct gaaaaagtac ctctccatca gagatttgga aaggatggtg
      841 cctgaatatc tgaaaggata taccgggaag gcaataccgg ctgcccctga aattgccctg
      901 gatattgata agcctgtaca actggaagtt ttaaaagaat tgacgagaaa gcaataatgc
      961 ttagagtttg ccctttacac catagatata cccgccagat cttcccacta taagattaga
     1021 ggtaatgaat gccttgggat catattccag tgctatatca cgagctttag gaaataaact
     1081 tcgttgtgtt ataattcgta aaacctgtaa ctctccttgt aatccataac caagatttct
     1141 ggtaacccca aatccttcct ctcgaagacg cttttctatc tctggccagt gtttcctgga
     1201 aatgacttcc actgatataa tttcatggga ggtcttctct gtaatcataa ttccaacaat
     1261 agtacccgta gcaaagcctc cacagtaacc aagaatattc atccagttgc ccacatcgtg
     1321 tataacttta gatacaatga ggatgaaaat aaatacttct acaaagccaa taatgccagc
     1381 caaaagcttt tttctactaa caatcatctg aatccgatat gtcccaaggg atacatcaat
     1441 gatcctaaaa aagaatataa gtaaagcgcc tatatatggg tttagattat gcatattatt
     1501 taacaaatca ttcattttgt tactcctttt atcaagtatc acgtatctgt tcccagtttt
     1561 aattcagaga ttgtctttat aggtcaagta gaagtttatt actttctatt gtataaaaca
     1621 atagaaaata ttcattttaa tttgagcaat taaaaaacta taatactgca aagcatatta
     1681 atataataaa tacagatagg taacctgaac ctgtatggga acaatgagag gttgattaaa
     1741 tcctgattag ccctttaatt ccattttata tattcaaaat aatacttata aaaattctta
     1801 tctttaactg tatttaacta cgttagccat atcttgaaga taattaaaaa ttttcgatac
     1861 ttttaatatt tacttaaagt agcaaacggg atacactact cctcttggtt gtattataat
     1921 atatgctaca atgtattaaa agacaatatt gttaataatt ttatttttca ggggaaaagg
     1981 agcgcaagca atgaatgcag agattatcag tattggcaat gagattttag gcggtaatat
     2041 agttgatacc aatcctaatt atatcgttaa aaaattaaag gagataaatc ttaatgtaca
     2101 acatatctcc gctgttggtg atgaagaaaa agaaattatc actcaattga aagaagctta
     2161 tcagcgttct cagattatta tcactaccgg cggacttggc cctaccgagg atgatataac
     2221 ctttcaatca gtagccagaa catttcaatt aaatctggtc aaatatccag aagcagaaag
     2281 acatatggtt gaaagaatca gcaaaattgg tgtttttgtt tcaccgagca atttaaagca
     2341 gagctttttg ccagaaggct ctacctgtat cattaacccc tatggaactg cgccggctat
     2401 gatacttgaa aaagaagaca gagtgataat ctcacttccc ggtgttcccc tggaaatgaa
     2461 aaatttatta tctgaacaaa taatcccttt cttacaaaaa aaatttccca atttagaaca
     2521 tctgcaatcc cgaattatca ggaccagcgg gctgggagaa tcaaatctca acgatatgat
     2581 taaagatttc atcttaagta atcagagatt gaatgttggt atttatgcca gcccagaaga
     2641 cattcaaatt caattaaatg ccctctctaa aactccggaa gagacaaaaa atattttaga
     2701 taaggctaca gagcaactaa aagttctgct gggggattac atttttggct ataatgatga
     2761 atctctagaa ggtgttattg ggaacctgtt gaaaagaaag aatttgactt tagctgttgc
     2821 cgaatcatgc accggaggaa tgctgggagg tgctatcacc gctgttcctg gcagttcaga
     2881 ctattttaaa ggtggcatta taagttatga tggagaaatt aaagaaaatt tattaggcgt
     2941 tccccgcagt attttaatca attttggaca ggtcagcgaa ccgactgcgc gggctatggc
     3001 agaaggtgtc cgaaaaatgt gtaacagtga tttaagttta agtattaccg gtattgccgg
     3061 tccggcagga ggaagtccag agaagccagt aggattagtc tatattgctc ttgctgacaa
     3121 cgaacaagtc ctggtgcaga aacatcaatt ccataatgac agacaggcta tccgtttacg
     3181 ttctgtgcga cgggcactta atatgctcag attatatc
//
LOCUS       DAOQWT010000137         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_31681_length_3215_cov_3.316139, whole genome shotgun sequence.
ACCESSION   DAOQWT010000137 DAOQWT010000000
VERSION     DAOQWT010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31681_length_3215_cov_3.316139"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1264
                     /locus_tag="PKJ95_03510"
     CDS             146..1264
                     /locus_tag="PKJ95_03510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNR65346.1"
                     /translation="MKINNKKTVKFKKEYSHNPLLILLIMLSVFFISFLSELSINASQ
                     LVYDELSIPILTYHKFCLGESTDEYTININHFKQQMHYLKKNDYRVISVSQLLECIEN
                     NVYPEKPVVLTIDDGYKSVYTLAFPVLKEYGFPVTLFLYTDFINNGPNQLSWSEIKEM
                     AAMGMEIGSHTLSHANLLNQKSNESYLDYINRIKKEIVLSKTILERNTGGTIISFAYP
                     YGVYSEEIRMLAKQAGYKALLNVNNMNNSIPIDPYSLNRQIIPNNGSLKQFGAIVQEK
                     TLQVNAIFPPDGTITDNQGITIGAILDNRNIEPTTLYLRLSGSGLLNHTYCPEQQKIS
                     FTPLAPKLLQKRTWIAQITARDTETGEQRKVSWLFTVR"
     gene            complement(1437..1655)
                     /locus_tag="PKJ95_03515"
     CDS             complement(1437..1655)
                     /locus_tag="PKJ95_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HNR65347.1"
                     /translation="MKQIGELEITDTSKLVLSFGEFRGAQRVDLRTYVKLKDKDEYIH
                     TKKGINFNAEWIDDFVKMIEKLKDVKLD"
     gene            complement(1709..2023)
                     /locus_tag="PKJ95_03520"
     CDS             complement(1709..2023)
                     /locus_tag="PKJ95_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65348.1"
                     /translation="MIDNHNQMPAKKILISNLNRIGIVHLHGIEKYLDRHDYQKCFLI
                     TPIAPHENVYKYAEKIPSLEIIISSDYAGEKKRIEEEHPEAKMFVIDAYHLAGRDGML
                     DA"
     gene            complement(2201..2845)
                     /locus_tag="PKJ95_03525"
     CDS             complement(2201..2845)
                     /locus_tag="PKJ95_03525"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HNR65349.1"
                     /translation="MIFLLFLYLLMPIFFFFIIALIFLPYGFTFFPLIMLISVPKQIW
                     KIAKSKNIRRNHAIEHATINILEQSSGYQLNISGLSQEDGFYITGIENPDIVERAAIR
                     GLALLKEGHCQLAIHRRCGTDIAIANFVTALIFLLLLFSTRAVSILNIVIALLLSNFI
                     SPYLGEYVQKYFTTSCEVANTEIAGLRYEIGRNERSMMLRYFPRGYFIKTETYS"
     gene            complement(3009..>3215)
                     /locus_tag="PKJ95_03530"
     CDS             complement(3009..>3215)
                     /locus_tag="PKJ95_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNR65350.1"
                     /translation="KTTGEILRALRSSKKAPGAERIYTAGEKEYLSTLEREKTGIPIN
                     KNLQKQLLTIQKELSLIQYQFTFS"
BASE COUNT         1086 a          577 c          475 g         1077 t
ORIGIN      
        1 aaaaaaattt ttaccaatgc gattttaaaa gaaatgaagt aaaagaaaat ataccaatat
       61 taaaaaatgt tctttaaatt gatgattatt gctaaatatt tttcagaaaa ataacaaata
      121 ataacttaat aatatataac tacaaatgaa aataaataat aagaaaacag taaaattcaa
      181 aaaagaatat agtcataacc cattgttaat attattaata atgctttcag tgttttttat
      241 ttccttttta agtgaactat caattaatgc ctctcaatta gtttatgatg agctttccat
      301 tcctatcctt acctaccata aattttgtct gggagaaagc acagatgagt acaccatcaa
      361 tattaaccac tttaaacagc aaatgcatta tcttaaaaaa aatgattacc gtgttatatc
      421 tgtctctcaa cttttagaat gcattgaaaa taacgtttac cctgaaaaac cagttgtgct
      481 caccattgat gatgggtata aatctgttta taccctcgct ttccccgttt taaaagaata
      541 tggttttcca gtcacccttt ttttgtatac tgacttcatt aataatggtc ccaatcagct
      601 ctcctggtca gagattaagg agatggctgc catgggaatg gaaattggtt cccatactct
      661 cagccatgcc aatctgctca atcaaaaatc aaatgaatca tatctcgatt atataaacag
      721 aataaaaaag gaaatcgtgc tctctaaaac cattctggaa agaaatactg gtggtactat
      781 tatctctttt gcatacccct atggggtata tagcgaggag attagaatgt tggctaaaca
      841 ggctggttat aaagccttgc tcaatgtaaa caatatgaat aactctatac ctatcgaccc
      901 ctattcactc aatcgtcaga ttatacctaa taatggctca ttaaaacaat tcggggcaat
      961 tgtgcaggaa aaaactttac aggtcaatgc catctttcca cctgatggta ccattactga
     1021 taatcaagga attaccatcg gggcaatatt agacaatcgt aatattgagc cgacaaccct
     1081 ctatttaaga ttgagcggct caggacttct aaaccatacc tattgccctg aacaacaaaa
     1141 aatttctttt acccctttag ccccaaaact tctgcaaaaa cgtacctgga ttgcccagat
     1201 aactgccagg gataccgaaa cgggagaaca gcggaaagtt tcctggcttt ttacggtgag
     1261 ataaagaggg caggaataga gcagggtatt ttatattaga ttaaagtaaa tgacatttcc
     1321 cttattctct taatagaata ataaaatatt aagatatttt atgccaatta caattattta
     1381 tttaccctta aaaataacac tgatattaat atcaatatat aaattataaa tactggttag
     1441 tccagtttga catcttttag tttttctatc atcttcacaa aatcatctat ccactctgca
     1501 ttaaagttta ttcctttttt agtatgaatg tactcatctt tatcttttaa ttttacatat
     1561 gttcttaaat caacccgttg cgcgcctctg aactctccaa aagacagaac taatttagaa
     1621 gtgtctgtaa tttctaattc acctatctgt ttcatatact actattattt cctttcttgt
     1681 ttatatgtta ttatattata tttatttttt aagcatccag cataccatct ctaccggcaa
     1741 gatggtaggc atcgataaca aacatctttg cttcaggatg ttcttcttca atcctttttt
     1801 tctctcccgc ataatcagat gaaatgatga tttctaaaga aggaatcttt tctgcatact
     1861 tatagacatt ttcatgggga gcgatggggg taataagaaa gcatttttga taatcatgtc
     1921 tgtctaaata tttttctatc ccgtgtaaat gaacaatccc aattcgattt agattggaaa
     1981 taagaatctt ttttgctggc atttgattat gattatcaat caccagtaat cctcctccct
     2041 tattttgagt tttctttatt ttttaaagga tatcttccct cgatcaaaaa attcttaacg
     2101 cccttatctc cctttgtttt tttatcatct gaatagtatc acaagaggca gataaattta
     2161 atattaatat ttttaaaatt aacttttctt cttacattaa ctatgaatag gtctctgttt
     2221 taataaaata acctcgcggg aaatatctta acatcatact cctctcattt cttcctattt
     2281 cataccgcag cccggcaatt tctgtatttg cgacttcaca ggaggtagtg aaatattttt
     2341 gtacatattc tcccaggtaa ggacttataa aattggagag gagtagggct ataacaatat
     2401 tcaaaatact gacagccctg gtggagaaaa gcaataaaag aaatattaga gcagttacaa
     2461 aattagcaat agcaatatct gttccacaac ggcgatgaat agccagctgg caatgacctt
     2521 ctttcaataa agcaagacca cgaatagcgg ccctttccac aatgtctgga ttttcaatac
     2581 cggtaatata aaaaccatct tcctgagaca ggccagaaat atttaactga taacctgatg
     2641 attgttccaa tatattgata gtagcatgtt caatagcatg atttcttctg atatttttac
     2701 ttttagcaat tttccagatc tgtttgggaa cagagattag cattatcagg ggaaaaaaag
     2761 taaacccata tggtagaaat atcaacgcta tgataaaaaa gaaaaatatg ggcattaata
     2821 aataaagaaa taacagaaaa atcaatttta cagaccttct ctcttcttaa tttatatttc
     2881 gattttaata ctgaattgac ttttttgtca aaaacataac cataataata attaattaga
     2941 aaactgtcaa ctcaagaagc attcaacaga gggaaaagat gaaaggaaga agggctcgct
     3001 tttgcctttc aggaaaaggt aaattgatac tgaatgaggc ttagctcttt ttggatagtt
     3061 aatagctgtt tctgcaaatt tttattgatt ggaatccctg ttttttccct ctccagagta
     3121 gatagatatt ctttttcacc ggcagtatat attctttccg cgcctggtgc ctttttagaa
     3181 gagcgcaaag ctctcagaat ctcaccggta gtttt
//
LOCUS       DAOQWT010000138         3206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_31822_length_3206_cov_2.832117, whole genome shotgun sequence.
ACCESSION   DAOQWT010000138 DAOQWT010000000
VERSION     DAOQWT010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31822_length_3206_cov_2.832117"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..705
                     /gene="efp"
                     /locus_tag="PKJ95_03535"
     CDS             148..705
                     /gene="efp"
                     /locus_tag="PKJ95_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNR65351.1"
                     /translation="MISTSDFKNGLSIELDGDLYTIIEFQHVKPGKGGAFVRTKLRNV
                     QSGLVIDRTFRAGEKFEQAIIERKDMQYIYNDGHNYYFMDQDSFEQIPISTEMIGASA
                     DLLKEGNSVEVSFCKEQVIGVQLPATVALQVVNTVPGAKGNTVSGAMKPATVETGAEI
                     QVPLFIKEGDIIRITTSDKKYQERV"
     gene            715..1143
                     /locus_tag="PKJ95_03540"
     CDS             715..1143
                     /locus_tag="PKJ95_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNR65352.1"
                     /translation="MDLATVITEKGKINFTRITIETLIRIVLQEIKGIIQPKKISFPK
                     IIQSFANENLETKKNITPEVRVEIKPDSIQVNLFLTIHYGLRIPDLTWEVQAKIKEKI
                     KEVTSLDIEQINVHIQSIRFSKKYHYKRKLVAPGSFLKIF"
     gene            1189..1614
                     /gene="nusB"
                     /locus_tag="PKJ95_03545"
     CDS             1189..1614
                     /gene="nusB"
                     /locus_tag="PKJ95_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNR65353.1"
                     /translation="MGSRRIAREMALKILFQVDLVHCNIDEASSYAIQIEDLSQYNNS
                     DDIISFCFELVKGVLPNLTEIDALIKKHANNWSLERMANIDRNILRIAIYEIVFVSNI
                     PKSVSINEAVELAKKYSTENSFGFVNGVLGQIEKKNEII"
     gene            1643..2881
                     /gene="xseA"
                     /locus_tag="PKJ95_03550"
     CDS             1643..2881
                     /gene="xseA"
                     /locus_tag="PKJ95_03550"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNR65354.1"
                     /translation="MLLNTSNERVFTVSSITRHIQTVFKEDPLLSDITIIGEISNYKP
                     HSSGHMYFILKDEQSQIKCVMFRGNNYGLDFKPMDGLSVRAKGEIRVYEKRGEYQLYV
                     SQMSEAGRGALFAAFEALKEKLKAEGLFSPETKKPLPRIPHKIAVITSPTGAAIRDII
                     SITLRRFPNMHIVVMPAQVQGDVAAEQIAENITFLNQVMPELDFIIIGRGGGSIEELW
                     AFNEEKLARAIYASEIPIISAVGHETDFTISDFVADLRAPTPSGAAEMAIPEKESLMK
                     HLSLIKNKVNRIFVHILELKNQRCLNLIENIKYQSPKNMILQHLQTVDDLYNRLKLNM
                     KYILDMNENNLIKYREKIISLSPKSILKRGYSICLKIPERVVVKNVKQVERDHKIEII
                     VQDGNISANVSGKEEIKNDR"
     gene            2871..3140
                     /gene="xseB"
                     /locus_tag="PKJ95_03555"
     CDS             2871..3140
                     /gene="xseB"
                     /locus_tag="PKJ95_03555"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNR65355.1"
                     /translation="MTDKKKKDEDISFEESLKKLEEIAEELEKGELNLDEALKLYEEG
                     MHFSDKCLEKLNETKKKVEKLTREGDGKYRTEPFSITEDEEASNE"
BASE COUNT         1175 a          468 c          680 g          883 t
ORIGIN      
        1 aaatagtagc agcatgactc ttgctttcag gctcatattt ggattagaaa agagttggac
       61 tgataattta acagaataat atggtatact ctctcatata atgagaatac aaaatttcac
      121 aatgaacaga ggtaaggagg ggttaacttg atatctacca gtgatttcaa aaatggttta
      181 agcatagaac tggatggcga tttgtacact attattgaat ttcagcatgt taaacctggc
      241 aaagggggag cttttgtcag aacaaaattg agaaatgtgc aaagcggatt agtcatagac
      301 cggacttttc gtgccggtga aaagtttgaa caggcaatca tagaacgcaa agatatgcag
      361 tatatttata atgatggcca caactattat tttatggacc aggatagttt tgaacagatt
      421 cctatcagta ctgagatgat aggagcctcg gctgatttat tgaaagaggg aaatagcgtt
      481 gaagtttcct tctgtaagga acaggtaatc ggagtacaac taccagcaac agttgcacta
      541 caggtggtaa ataccgtacc gggggcaaaa gggaatactg tatcaggcgc tatgaaaccg
      601 gcaactgtag aaaccggtgc agagatacag gtgccccttt ttattaaaga aggggatatc
      661 atcagaataa ccaccagtga taaaaaatat caggaaagag tataaaaaag aaatatggac
      721 ttagcaacag ttattactga aaaaggaaaa attaatttta cccgaattac cattgagact
      781 ttaatccgta ttgttttaca ggaaataaaa gggattattc aaccaaaaaa aattagtttt
      841 ccaaaaatta tccagtcatt tgccaatgaa aatcttgaga caaagaaaaa tattactcca
      901 gaagttagag tggagataaa acctgattcc attcaggtca atcttttttt aacaatacac
      961 tatggattga gaattccgga tttaacctgg gaagtccagg caaagataaa agaaaaaatt
     1021 aaagaagtaa ccagcctgga tatagagcaa atcaatgttc atatccagag tattcgtttt
     1081 tctaagaaat accactataa gagaaaattg gtcgcgccag gcagtttctt gaaaatattc
     1141 taaaagataa agtatagttt gataatagaa taagggtttg aggaaattat ggggagcagg
     1201 agaatagccc gggaaatggc actgaaaatt ctatttcagg tagaccttgt tcattgcaat
     1261 atcgatgaag caagttcata tgccattcag attgaagatt tgtcgcaata taataattca
     1321 gatgatatca tctctttttg ctttgaatta gtaaaaggtg tactgcccaa cctgactgag
     1381 atagatgcat taattaaaaa acatgccaat aactggtcat tagagagaat ggctaacatt
     1441 gaccgtaata tattacgtat agccatctat gagatagttt ttgtgagcaa tatccccaag
     1501 agcgtatcta ttaatgaggc agtagagttg gctaaaaaat atagtactga aaattccttc
     1561 ggctttgtca atggcgtcct ggggcagatt gaaaaaaaga atgaaataat ttgaattttt
     1621 taagataaaa acagggattg agatgctttt aaacacttcc aatgaaagag tatttacggt
     1681 tagcagtatt acccgccata ttcaaaccgt atttaaagaa gacccccttt taagtgatat
     1741 tactattatc ggtgagataa gtaattataa accacactct tccggacata tgtattttat
     1801 tttaaaagac gaacaatcac agataaaatg cgtaatgttt cgaggcaata attatgggct
     1861 agattttaaa cctatggacg gattaagtgt aagggcaaag ggagagataa gggtttatga
     1921 gaaaagagga gaatatcaat tatatgtttc acagatgagt gaagcgggca gaggcgcctt
     1981 atttgctgct tttgaggctt taaaggagaa attaaaagca gaaggattgt tcagtcccga
     2041 aaccaagaag cccttaccca gaattccaca taaaattgct gttataacct cacctaccgg
     2101 agctgccatc agagatatta tttccattac cttacgccgc tttcccaata tgcacattgt
     2161 tgtaatgccg gcacaggtgc agggagatgt ggcagcagag cagattgctg aaaatattac
     2221 ttttcttaat caggtaatgc cagagctgga ttttatcatt atcgggaggg gcggaggttc
     2281 tatagaagaa ttatgggctt ttaatgagga gaaattagcg agagcaatat atgcttctga
     2341 aattcccata atatcggcag ttggccatga gactgatttt actatatctg attttgtggc
     2401 agatcttcga gctcctacac cttccggggc ggctgaaatg gccatccctg aaaaagaaag
     2461 tttgatgaaa catctctcct tgataaaaaa taaggtaaat cgtatttttg tacatatttt
     2521 agagttaaaa aaccagagat gtcttaatct aatagagaat ataaaatatc aaagccctaa
     2581 aaatatgatt cttcaacatc tgcagactgt tgatgattta tacaatcggt taaagcttaa
     2641 tatgaagtat attttagata tgaatgaaaa taatctgata aaatacagag aaaagattat
     2701 ttctttgagc cccaaatcaa tattgaagag gggctatagc atctgtttga aaattccgga
     2761 aagggtagtg gtgaaaaatg taaaacaggt ggaaagagac cataaaattg aaatcattgt
     2821 tcaagatggt aatatatcgg ctaatgtttc tggtaaggag gaaataaaga atgaccgata
     2881 agaaaaaaaa ggatgaagac atatcttttg aggaatcact gaagaaactg gaagagatcg
     2941 cagaagaatt ggaaaaaggt gagcttaatt tagatgaggc attgaagtta tacgaagagg
     3001 gaatgcattt ctctgacaaa tgtttagaaa aattgaatga aacgaaaaaa aaggtggaga
     3061 agctgaccag ggagggtgat ggaaaatatc gtaccgaacc attttctatt accgaggacg
     3121 aagaggcaag caacgagtaa atggaattaa gtgactactt aaaagagaaa aagaaaatta
     3181 ttgataaata tctggaagag aaactt
//
LOCUS       DAOQWT010000139         3193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_32006_length_3193_cov_3.290631, whole genome shotgun sequence.
ACCESSION   DAOQWT010000139 DAOQWT010000000
VERSION     DAOQWT010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32006_length_3193_cov_3.290631"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /gene="rpsE"
                     /locus_tag="PKJ95_03560"
     CDS             <1..139
                     /gene="rpsE"
                     /locus_tag="PKJ95_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568726.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNR65356.1"
                     /translation="DILTKSLGSNNHLNVVKATTDALLSLRSAREIAKLRGIPVEKIF
                     N"
     gene            167..607
                     /gene="rplO"
                     /locus_tag="PKJ95_03565"
     CDS             167..607
                     /gene="rplO"
                     /locus_tag="PKJ95_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNR65357.1"
                     /translation="MKMNSLKPNPGSTKSRKRVGRGNSSGHGSTSGRGTKGQKSRSGS
                     KISSYFEGGQMPLSRRVPKRGFRNIFRNKYAIINIAQLNKFDDGEVITPERLIQDGMV
                     KKLLSGIKILSEGEITKKITIKAHKFSKEAINKIEAAGGKVEVI"
     gene            608..1873
                     /gene="secY"
                     /locus_tag="PKJ95_03570"
     CDS             608..1873
                     /gene="secY"
                     /locus_tag="PKJ95_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005377189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNR65358.1"
                     /translation="MLEQLRNIFKIPDLKNRLLFTLAMLIVVRLGSHIPLPGIDKQAL
                     ANLFAQGGILGFFDLFAGGALSRFSLFALGIMPYINASIIMSLLQSIVPLFEQWSKEG
                     EEGRNKINSITRYGTLVLGLIQAVGISAWLESLQVLIISGIMFRILLTVSLTAGTCFL
                     MWIGEQISDFGIGNGISIIIFTGIVARIPTEIIQTWSLVRVGEVGIISIIALLAVIVL
                     VIAGVVAIQQGQRRIPVQYAKRVVGRRLYGGQGTHIPLRVNQAGVIPIIFASAILIFP
                     GTLAQFFQGSTFMQKIAQALSPGTPLYLTLYAILIIVFTFFYTAITFNPQNLSDNIKK
                     YGGFIPGRRPGKATAMYIDSIMTRITLSGAIFLAIIAILPNILIEVTGITTIYFGGTS
                     VIIMVGVALETVEQIESHMLMRHYEGFIK"
     gene            1914..2603
                     /locus_tag="PKJ95_03575"
     CDS             1914..2603
                     /locus_tag="PKJ95_03575"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNR65359.1"
                     /translation="MIIILLGPPGAGKGTIANEIQRRIVIPVIATGDILRNAIDKNTE
                     IGMKAKNFVRSGKLVPDEYVIKIVENRIKEFDCEKGFILDGFPRNINQAKALDKVFER
                     LDKKIDQVFYFEASYQNIINRLSARRVCTKCGTIYNLKYDPPDKAELCHKCNGKLIQR
                     DDDREDTIKKRLDVYNDETLPLSNYYRKKRILTKINANQDVIAILKDIWNRLAELGIG
                     ENRNDYGNYKN"
     gene            2584..>3193
                     /gene="map"
                     /locus_tag="PKJ95_03580"
     CDS             2584..>3193
                     /gene="map"
                     /locus_tag="PKJ95_03580"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNR65360.1"
                     /translation="MVIIKTNKEIKNIKKSCQLAANTLKYLAERIKPGLKTIQLDQAA
                     ELFIKKHSGVPSFKGYGPKGYEFPASICVSINDEIVHGIPGERVLKEGDIISLDIGTN
                     LNGYFGDMAETFGVGEIDCKAKKLINTTKECLFAGIKQAVKGNYIGDISFAIQSIAEK
                     NGFSVVREYVGHGVGLMVHEDPQIPNYGRRRTGLKLEEGMVIA"
BASE COUNT         1108 a          451 c          653 g          981 t
ORIGIN      
        1 ggatattctt accaaatctt taggaagtaa taatcattta aacgtggtta aggccactac
       61 agatgcttta cttagcttaa gaagtgccag agaaattgct aaattaagag gaattccagt
      121 agaaaaaatc tttaattaaa atagttgttg ccaaaggaga tttgctatga aaatgaatag
      181 cttaaaaccc aaccctggtt ctacaaaatc aagaaaaaga gtgggtaggg gaaattcttc
      241 aggccatggt tccacttctg gaagaggaac aaaaggtcag aaaagtcgtt ctggttcaaa
      301 aataagttca tattttgaag gtgggcaaat gcctttaagt agaagggtgc ccaaacgtgg
      361 tttccgtaac atttttcgga ataaatatgc cattataaat attgcgcaat taaataaatt
      421 tgatgacgga gaagtaatta ctcctgaaag attaatacag gatggtatgg taaaaaaatt
      481 attgagtggt ataaaaattc tgagcgaagg tgaaatcact aaaaaaatta ctatcaaagc
      541 ccacaaattc agtaaagaag ctataaataa gattgaagca gctggtggaa aggtcgaggt
      601 gatataagtg ctggaacagc ttcgtaatat atttaagatt cctgatctga aaaatcgttt
      661 attatttact ttggcaatgc taatagtagt aagactaggg agtcatatac cattaccagg
      721 tattgataag caagcattgg caaatttgtt tgctcaaggt ggtatacttg gtttttttga
      781 tctttttgcc ggtggtgcgt tgagtagatt ttcactgttt gctcttggca ttatgcccta
      841 tattaatgcc tcaattataa tgtctttatt acaatcaata gtacctttat ttgaacaatg
      901 gtctaaagaa ggcgaagaag gtagaaataa aataaatagt attaccagat acggaactct
      961 tgtacttgga cttatacagg ctgtaggcat tagcgcctgg ttagaaagtt tacaggtttt
     1021 aattataagc ggtattatgt ttagaatatt attgactgtt agtctaactg caggtacctg
     1081 ttttttaatg tggataggtg agcaaattag tgattttggt atcggcaatg gtatttcaat
     1141 tattatattt accggtattg tagccagaat ccctactgaa attattcaaa cctggagttt
     1201 agtcagagta ggggaagttg gtattatatc cataattgct ttgctagcgg ttattgtgtt
     1261 agttatagcc ggagtagttg ctatacagca aggccagaga cggataccgg tgcaatatgc
     1321 caaacgtgtt gtgggcagaa gattatatgg agggcaaggc actcatatac cattacgtgt
     1381 aaaccaagct ggtgttatcc ccattatttt tgcttctgca attttaattt tccctggtac
     1441 tctggcacaa ttttttcaag ggtcgacctt tatgcaaaaa atagcccaag ccctttcacc
     1501 gggaacacca ttgtatctga ctttgtatgc tattttaata attgtcttta ccttttttta
     1561 cactgccatt acttttaatc cgcagaactt atccgataat attaaaaaat atggtggctt
     1621 cattcctgga cgaagacctg gtaaggcaac tgctatgtac attgacagca taatgaccag
     1681 gataacttta agtggcgcaa tattcttagc tataatagcc attttaccca atatattaat
     1741 agaagttacc gggattacta ctatttattt tggcggcacc tcagttatta ttatggtggg
     1801 cgttgctctt gaaacagttg agcagattga atcgcatatg ttaatgaggc attatgaagg
     1861 atttatcaaa taacaaaata caagtttcct ctgacttttt tgagggatga aaaatgataa
     1921 ttatactatt gggtccaccg ggtgctggca aaggtaccat tgccaatgaa atacagaggc
     1981 gaattgtaat tccagtaatt gctactggtg acattttgag gaatgctatt gataagaata
     2041 ccgaaatagg aatgaaagca aaaaattttg taagatcagg taaattggtc cccgacgaat
     2101 atgttattaa aatagtagag aatagaatta aagaatttga ttgcgaaaaa gggtttattt
     2161 tggatggttt tcccagaaat attaatcaag caaaagcgtt ggacaaagta tttgaaagat
     2221 tagataaaaa aattgaccag gttttttatt ttgaagcttc ttatcagaat attattaatc
     2281 gtttatcagc gagaagagtt tgcaccaaat gtggaactat ttataatctt aaatatgatc
     2341 cacctgataa ggctgaattg tgccacaagt gcaatggaaa attaatccaa agagatgatg
     2401 accgagaaga tactattaaa aagagattag atgtttataa tgatgagaca ctacctttat
     2461 caaattatta tcgaaaaaag agaattttaa caaaaatcaa tgctaatcaa gatgttattg
     2521 ctatacttaa agacatatgg aatagattag ctgaacttgg tattggcgaa aacaggaatg
     2581 attatggtaa ttataaaaac taataaagag attaaaaata ttaagaaaag ttgccaactt
     2641 gcagcaaata ccttaaaata tttagctgaa agaataaaac ctggcttaaa aacaatacag
     2701 ttagaccagg ctgcagagtt attcataaag aaacacagcg gtgtaccatc ttttaaagga
     2761 tatggtccca aaggttatga gtttcccgca tcaatatgtg tttctataaa tgatgaaatc
     2821 gttcatggaa taccaggaga acgggtatta aaggaaggtg atatcatcag tctcgatatt
     2881 ggcactaatc ttaatggata ttttggtgat atggcagaaa cgtttggagt tggcgagata
     2941 gattgtaaag caaaaaagtt aataaataca acaaaagaat gtttatttgc tggtataaaa
     3001 caggcagtaa aaggaaacta tatcggagat atatcatttg ctattcaatc aattgctgaa
     3061 aaaaatggtt tttctgtggt gcgagaatat gttggacatg gtgtaggatt gatggtacac
     3121 gaagaccccc aaatccccaa ttatgggaga cgaagaactg gattaaaatt agaggaagga
     3181 atggtaatag cca
//
LOCUS       DAOQWT010000140         3190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_32050_length_3190_cov_2.844019, whole genome shotgun sequence.
ACCESSION   DAOQWT010000140 DAOQWT010000000
VERSION     DAOQWT010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32050_length_3190_cov_2.844019"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1871)
                     /locus_tag="PKJ95_03585"
     CDS             complement(150..1871)
                     /locus_tag="PKJ95_03585"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HNR65361.1"
                     /translation="MKGIGKTVFSGTRIEEFGVEVIDIVHGSGIANPFILVRLSGDRI
                     EKNGGISAGMSGSPVYFDGKLAGAISHAWEMSEHNLCLITPIDLMVNLFNYAGKENER
                     ANFSSSFNDDIDIVSLFIDESLHRKLSNLEYLSDKADYCFSNNEKVIFNFQLLQSPLL
                     ISTSSRRAGHYVEEILSKHGITLVENIFEYENIKPELEINTDAKNILPGSAIGVQLST
                     GDISIIGIGTATYCQDKYVLALGHPFMHRGDVSYLFTSVYIYHSFPSIVMPFKIGSPY
                     LLLGEVIQDRNAGILARLNKFPSIVSCKIRVNDLDRNIDIGSGVKIVSEKDIVQSVVP
                     ALLIQSVDNAINRIGQGTGAIRYSFRNAGTGEIINYNNIFFSENDVALEITKDLNTLF
                     DLLYYNFYEKIDLNEIMIDVAIKKQNNRAYIKEVKLDNEDYSPGDEIEAQIIILPFRQ
                     AAEQKEISIKLPDDINPGNAVLLVKGGSSKEEINEQSIPRNDQYYLLNGWSEIEKYLI
                     YQEKNNQIIAEIILLNDNEEFDDSVKSESDKNKNNLKTIIDTDFVMDGYQEVFFNIKK
                     NPIDELE"
     gene            complement(2062..3081)
                     /locus_tag="PKJ95_03590"
     CDS             complement(2062..3081)
                     /locus_tag="PKJ95_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNR65362.1"
                     /translation="MLGMEKSLGIDLGTVSILIYQKGKGIVLQEPSVVSIYKDTGKVF
                     AVGKEAKEMLGKTPGNIIALEPLKAGVIADYDVTEKMLTYFIKKVSSNSRLFRPQVVI
                     CVPAGGTEVEKRAALEAAIQAGAKKAFLVEESIAAAIGAGLDITEPYGNMVVDVGGGT
                     TDIAVISLGGIVESESLRIASNNFDEEIIKYIKNKYNLMIGEKSAENLKIEIGTAIEP
                     ENEMFAEIRGRDLISGLPKTIEVGSSEVLEAITKSLQEILDAIRMVLERTPPELAADI
                     ADKGMVLTGGGSLLRNFDVLLTKVNKIPAYVAENPISCVALGAGKVLDQIHLLKQGLI
                     TSSHR"
BASE COUNT          987 a          638 c          416 g         1149 t
ORIGIN      
        1 tcaatttttt ctgattcttc aaatttatct aaagtataag tgagaagcgg tctccccaaa
       61 agtggtacta acatcttatt attagaattg caaaatcttg ccccatttcc agctgctgca
      121 atgatagcaa aatatttttt caaatttctt tactccaact catctatagg atttttttta
      181 atattaaaga atacctcctg atatccatcc atcacaaaat ccgtatctat aatagttttt
      241 aagttatttt tgtttttatc cgattcgctt ttaactgaat catcaaattc ttcattatca
      301 ttaagcaaaa taatttctgc aattatttga ttattttttt cctgataaat taggtatttt
      361 tcaatttcag accaaccatt tagtaagtag tactgatcgt ttcggggaat agattgttca
      421 ttaatttctt cttttgatga acctcctttg accaataaca ctgcgttacc cggattaata
      481 tcatcaggta gtttaattga aatctctttc tgttctgcag cctgtctgaa aggcaaaatt
      541 atgatttgcg cttcaatctc atctcctgga gaataatctt cattatccaa cttaacttct
      601 ttaatgtaag ctctattatt ttgcttcttt atagcaacat ctatcattat ttcatttaaa
      661 tctatctttt cgtaaaaatt gtaatacaat aaatcgaata aagtatttaa atctttagtg
      721 atttctaatg caacatcatt ttcactaaaa aatatattat tataatttat aatttctccg
      781 gtacctgcat ttctaaaact gtatctaatt gcccctgttc cttgccctat tctatttatt
      841 gcattatcaa cagattgtat caaaagtgca ggtacaacgc tttgcacaat atctttttcc
      901 gacactattt ttactccaga acctatatca atatttcggt ctaaatcatt aactcttatt
      961 ttgcaggaaa ctatactggg aaatttgttt aatctggcta atattcctgc atttctatct
     1021 tgaatgacct ctcccaataa aagataaggt gaccctattt taaaaggcat aacgatactg
     1081 ggaaagctgt gatatatata tactgaagtg aaaaggtatg aaacgtcacc tcgatgcata
     1141 aaaggatgcc ccaatgctaa tacatactta tcttgacaat aagtggcagt tcctatccct
     1201 ataatgctaa tatctccggt agatagctgt actccaatag ctgaaccagg tagaatattt
     1261 ttagcatctg tattaatttc taattcgggt ttaatgtttt catattcaaa aatattttca
     1321 actaaggtaa tgccatgctt gcttagaatc tcttcaacat aatggccagc tcgtctgcta
     1381 gatgtactaa tcaatagggg agattgtagg agttgaaaat taaaaattac cttttcatta
     1441 tttgaaaagc aatagtctgc tttgtctgat aaatattcta aatttgataa ttttctatga
     1501 agactttcat ctatgaatag ggacacaata tcaatatcat cattaaatga gctactgaaa
     1561 tttgctctct cattttcttt cccggcataa ttaaaaagat taaccattaa atcaatgggt
     1621 gttataaggc ataaattatg ttcactcatt tcccaggcat gcgatattgc tccagctaat
     1681 tttccatcaa agtataccgg acttccactc attccagcgg aaatcccacc atttttctca
     1741 attctgtctc cgcttaatct tactaatata aaaggattgg caattcctga accatgaacg
     1801 atatctataa cctcaacccc gaattcttca attcttgtgc cggaaaaaac tgtttttcca
     1861 atccccttca ttcctggatg tagatcatta attagaatga actctccctg gtttccagca
     1921 attgcgtttc caggattata aaaaaaataa aataataata ttattaaaat ataaaaaatt
     1981 agaataaaga actttttata taattggctc atcttaaaca ataacataat aatattcttt
     2041 ttaattttta aagtaagctg tctatcggtg gctactggtg atcaacccct gttttagtaa
     2101 atgaatttga tccaatacct taccagcacc taaagcaaca caggaaattg gattttcagc
     2161 aacataagca gggattttat ttactttagt taataaaaca tcaaaatttc tcaacaaaga
     2221 acctccaccg gtcagaacca ttcctttatc agctatatct gcagctaatt cgggtggcgt
     2281 tctttctaaa accatcctga tggcatctaa aatttcctgt agactctttg tgatcgcctc
     2341 cagcacctca gaagagccca cttctatggt tttcggtaat cccgaaataa gatccctccc
     2401 tcttatttca gcaaacattt cattttccgg ctctatcgcg gtaccgattt ctatttttaa
     2461 attttcagcg gatttttctc caatcattaa attatattta ttcttaatgt atttgattat
     2521 ttcttcatca aaattgttac tggcaattct taatgattcg ctttccacaa ttcctcctaa
     2581 agagataact gcaatatctg tcgtgcctcc gcctacatca actaccatat ttccataggg
     2641 ttcggttatg tctaaaccag caccgatagc cgctgctata gactcttcta ccagaaatgc
     2701 ttttttagct ccagcctgta tggctgcttc gagggctgct cttttttcta cttccgtacc
     2761 ccctgccggg acacatatga ccacttgtgg acgaaataaa cgtgaattag aagatacctt
     2821 ttttataaaa taggttaaca ttttttcagt aacatcataa tcggctataa ccccagcttt
     2881 taaaggttct aaggcaataa tattaccggg tgtttttcca agcatctctt ttgcttcttt
     2941 gccaacagca aatacttttc cagtatcttt atatatagat acgacagaag gttcttgcaa
     3001 cactatccct ttaccttttt gatagattaa tatactgact gtacctaaat caattcctaa
     3061 acttttttcc attcccagca tacgtgatac tacctcccca taatctcaat tgctactcag
     3121 ttgttactat agaagaaata tgaatcaaac tatttattat tttaccataa ataaaatatt
     3181 ttttgttcta
//
LOCUS       DAOQWT010000141         3180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_32190_length_3180_cov_3.435520, whole genome shotgun sequence.
ACCESSION   DAOQWT010000141 DAOQWT010000000
VERSION     DAOQWT010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32190_length_3180_cov_3.435520"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKJ95_03595"
     CDS             complement(<1..463)
                     /locus_tag="PKJ95_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HNR65363.1"
                     /translation="MIKNRCTMETTLLFSEVGREHIPVIRFPGLSKYEEILHFITTRH
                     GGYSEKPFHSFNLASHVGDREENVLKNRECLARSLGIRAEKFFFTRQEHGNRIFLINE
                     ENSFYSNNRLTDTIPVSDAMITDCRDICLLIFVADCVPVLLFDCRKKIIGLV"
     gene            complement(487..1200)
                     /locus_tag="PKJ95_03600"
     CDS             complement(487..1200)
                     /locus_tag="PKJ95_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNR65364.1"
                     /translation="MVAQKNNNFEGFSAETVFFLKNLKENNNREWFLARKKDYSKKVL
                     QPAVIFVIEMGDMLQKISPGVQADPRINRSISRIYRDTRYSNVQEPYRAYLGLWFWQG
                     ARKTKDSSGYYFELRPEQLHLSAGIATFSRKALHLFRDAVIDTESGAELKKIINKIKE
                     NQNYLINKASYKNIPEGYQISDEQLKELIRFNGLAVHTEMPLPPELYNRNLLKLVFNI
                     YKDWSPLYNWLTQYVYNEK"
     gene            complement(1237..1815)
                     /locus_tag="PKJ95_03605"
     CDS             complement(1237..1815)
                     /locus_tag="PKJ95_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNR65365.1"
                     /translation="MDKYIPTRDEALQLLKEYNPDESLIKHALAVEGVMRYMARKNGE
                     DEEKWGIIGLVHDLDYYKYPEQHCQKSKEILEERGWPEEYIRAVISHGWGICSDVKPE
                     TNLEKTLYAIDELTGLVVTTALVRPSKSVMDMEVKSVQKKWKDKSFAAGVNRDIILKG
                     AELLGVGLSQLIDDTIKGMREVAGEIGLKGAL"
     gene            complement(2163..2783)
                     /gene="pyrE"
                     /locus_tag="PKJ95_03610"
     CDS             complement(2163..2783)
                     /gene="pyrE"
                     /locus_tag="PKJ95_03610"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNR65366.1"
                     /translation="MEDLLLKEKGHNMLNFEEKEVLEILKKTGAILNGHFLLTSGTHS
                     DGYIQCARILQYPSYARRVLELIAEKVKDLDMEIVVGPALGGIIVAYELGRQLNKRAM
                     FAERKDGIMQFRRGFVIKPGEKVLITEDVVTAGTSTMEVKKLVEERGADVIGVACIVD
                     RRSPEFNLLDLPLYSALKMNINTYAPDKCPLCKMGKPLENPGSRFI"
     gene            complement(2803..>3180)
                     /locus_tag="PKJ95_03615"
     CDS             complement(2803..>3180)
                     /locus_tag="PKJ95_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNR65367.1"
                     /translation="AQCCEEEGADGLSLVNTFKAMVIDIDRRKPVFDNIYAGLSGPAI
                     KPIALRMVHEVCQKVSIPVMGMGGICSGRDALEFIMAGASCVQVGTANFIHPHSCLKI
                     IAEIDDFLEAAGIQSLEEIRGII"
BASE COUNT          944 a          692 c          490 g         1054 t
ORIGIN      
        1 gaaccagccc aataatcttt tttctgcaat caaagagcaa taccggtaca caatcggcaa
       61 caaaaattaa aagacagata tctctacaat cagtaatcat ggcatcactg accggtattg
      121 tatcagttaa acgattgttg ctataaaatg aattctcttc attaatgaga aatatcctgt
      181 tgccatgttc ctgcctggta aagaaaaatt tttctgctct tatccctaaa gagcgggcta
      241 aacattcacg gtttttaaga acattctcct ccctgtctcc cacatgtgaa gccagattaa
      301 aggaatggaa gggtttttca ctataaccgc catgtcgggt agtgataaaa tgtagaatct
      361 cttcatattt agataaaccg gggaagcgaa ttacgggaat atgctctcta ccaacttcag
      421 aaaataacag ggttgtttcc atagtgcatc tgtttttaat cataattaat acgattgttt
      481 tggtcgttat ttttcgttat agacgtattg agtcagccag ttatagagag gtgaccagtc
      541 tttataaata ttaaaaacca gttttagcag atttctgttg tatagttccg gagggagggg
      601 catctcagta tgtacggcaa gcccgttaaa acgaattaat tctttcagct gttcatcact
      661 aatctggtac ccttctggaa tatttttata agaggcttta tttattagat aattctggtt
      721 ttcttttatc ttattaatta tcttttttaa ttctgcccca ctttcggtat caataactgc
      781 atcgcggaat aaatggagtg ctttgcgtga aaaagttgct atacctgcag atagatgtaa
      841 ttgttccggt ctcaattcaa aataatagcc agaggaatcc tttgtttttc tagcaccttg
      901 ccagaaccag agacctaaat aagcccgata aggttcctgg acattggaat acctggtatc
      961 ccgataaata cgtgatatgg agcgatttat acgtggatca gcctggactc cgggtgatat
     1021 tttttgcagc atatcaccca tctctatcac aaaaataacg gcaggttgta ataccttttt
     1081 actgtaatct ttttttcgtg ctaagaacca ctcacggtta ttattctctt ttaaattttt
     1141 tagaaaaaag acagtttcag cagaaaaacc ctcaaaatta ttattttttt gtgccaccat
     1201 aacaacattc ctccactgat aattcaggca ggtatgttaa agagcccctt ttaaaccaat
     1261 ttctcctgct acctctctca taccttttat agtgtcatca atcaattggg aaagacctac
     1321 acccagtagt tcagcacctt ttaagataat atcacggttt accccggctg caaaactctt
     1381 atccttccat ttcttttgaa ctgatttgac ttccatatcc atcacacttt tggatggcct
     1441 taccagagct gtagttacca ccagacctgt taactcatct atggcataaa gtgtcttttc
     1501 caggtttgtt tcaggtttaa catctgaaca tataccccac ccatggctga ttacagcacg
     1561 gatatattct tccggccagc ctctttcttc cagtatttct ttgctcttct gacaatgctg
     1621 ttccggatat ttatagtaat cgaggtcgtg gactaatcca ataattcccc acttctcttc
     1681 atcctcacca tttttcctgg ccatatacct cattacacct tctaccgcca gggcatgttt
     1741 aataagactt tcatcagggt tgtactcttt tagcaattgc aaggcttcat ctctggtagg
     1801 tatatactta tccatatttt aaccacctca tatttattac aattcaatta tggtactgct
     1861 acttattgct atacattata gcattatatg gttagaatta aaagtaaact actcgacagt
     1921 actttttaag atatttagga cacttttcat ttaggaattt tagatagtat ttttggagga
     1981 tttttgacta ttccggcagg aatattatca agaatatgaa aatgccttta aagatggggt
     2041 taatagaaaa ttgtttttta atttcctatt aatcccccaa cttaaaagta aaaaacgaaa
     2101 aatatataaa agcacatatt tattataaaa aattaaaaaa attgttttcc tgaattgatt
     2161 ttttagataa agcgactccc cggattttcc agaggttttc ccatcttaca aaggggacac
     2221 ttatcaggag cataggtatt tatattcatt tttaaagcac tatagagagg taaatctaga
     2281 agattaaatt ccggacttct tctgtcaaca atacaggcta ctcctatcac atctgctcct
     2341 ctttcttcca ccaatttctt cacttccatg gtagaggtgc cagcggtaac aacatcctct
     2401 gtaatcagta ccttctcacc cggctttatt acaaaacccc ttcggaattg cataattcca
     2461 tctttacgtt ctgcaaacat tgccctctta tttaattgtc tccccagttc atatgctaca
     2521 attatacctc ctaaagccgg tcctaccaca atttccatat cgaggtcttt aactttttca
     2581 gcaatcagtt ccagcaccct cctggcataa gaagggtatt gtaaaattct agcgcattga
     2641 atgtaaccat cgctatgagt accagaggta agcaaaaaat gtccatttaa tatcgcccct
     2701 gtctttttaa gtatttctaa aacctctttt tcttcaaaat tcaacatatt atgacccttt
     2761 tcttttaata ataaatcttc catattgcct atcctcccat atttatataa ttcctcgaat
     2821 ctcttctaat gattgaatcc ccgctgcttc cagaaaatca tcaatctccg caataatctt
     2881 cagacaggaa tgaggatgta taaaattagc agtacctacc tgtacacagc tcgctccagc
     2941 cattataaat tcaagggcat ctctcccact gcaaatgccg cccattccca taaccggtat
     3001 acttaccttc tggcaaactt catgaaccat acgaagggca atcggtttaa tagcagggcc
     3061 tgaaagaccg gcatagatgt tgtcaaagac cggttttctt ctatcaatat caataaccat
     3121 agccttaaag gtattaacta aggataatcc atcagcacct tcttcctcac aacattgagc
//
LOCUS       DAOQWT010000142         3165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_32434_length_3165_cov_2.963987, whole genome shotgun sequence.
ACCESSION   DAOQWT010000142 DAOQWT010000000
VERSION     DAOQWT010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32434_length_3165_cov_2.963987"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..422)
                     /locus_tag="PKJ95_03620"
     CDS             complement(192..422)
                     /locus_tag="PKJ95_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65368.1"
                     /translation="MITVKCIKCKSNIFKYQKIGKGKLLHCWKGRIKRDYSIHNGKEI
                     RCICGNVVGIEEEKWIKMKQHTFEISGTITKK"
     gene            complement(455..1378)
                     /gene="yddG"
                     /locus_tag="PKJ95_03625"
     CDS             complement(455..1378)
                     /gene="yddG"
                     /locus_tag="PKJ95_03625"
                     /inference="COORDINATES: protein motif:HMM:NF008676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid DMT transporter YddG"
                     /protein_id="HNR65369.1"
                     /translation="MVAKELNKKDNSTLLGILAILFWGSTVAFSRSLTEQLGVVTTSG
                     LVFISGGLLSWFYLIIRSPHTIKKMIHLPYNYLWGCGFLFVLYMISLYLAIGLSFNNQ
                     QVIEVSIINYLWPGLTLLFSVPILKKKAYFLIVPGTILSFAGVYIGMNPYHSLSIYSF
                     FQNIKNNSLPYILAFIAALSWALYSNLARKWAARDKEGAVPIFLLITGLLLLILRIFY
                     QEESRWTIRAGIELLYMAVFPTFLAYSFWDRAMRRGNILLVASLSYFTPLLSVFISSL
                     YLHITIEVRLWIASLLVVAGAFLSRYSISDA"
     gene            complement(1437..1964)
                     /locus_tag="PKJ95_03630"
     CDS             complement(1437..1964)
                     /locus_tag="PKJ95_03630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HNR65370.1"
                     /translation="MSKTNSLKRNEASNLKEELFCSECQKRINPEQFFYVDEKPVCYR
                     CIFGDVKPVSIYPIGFVKSNEPWGNAVIALYPQQKRFMYKLEEEKEICVVFYFHEIDA
                     IKTVFNRGNKSDGKRVGVFASRTPRRTSRIGITYVELLQVSGLELKVKGLDAFQGSPV
                     LDIKAIGKIKNYNLN"
     gene            complement(1957..2442)
                     /locus_tag="PKJ95_03635"
     CDS             complement(1957..2442)
                     /locus_tag="PKJ95_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNR65371.1"
                     /translation="MRRKDREISSREEIKDIIKKALFCNVAMCKDNAPYLVPMNFGFD
                     GQYFYLHSAPEGLKIDILKENPRVCIEITQDLKINQSEDVCKTSMRYSSVIIFGKVEF
                     LFDRRERINALNCIVEQLYGERATTKEEKPVFNEDNLNKLTILRVRPEHITGKRSVSS
                     E"
     gene            complement(2479..>3165)
                     /locus_tag="PKJ95_03640"
     CDS             complement(2479..>3165)
                     /locus_tag="PKJ95_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNR65372.1"
                     /translation="VADQMKGAPSPGLQLPYPETGEIISLPPWDKIQVSEDNIISIIN
                     VRQSIRNYKRENINLLELSYLLWCTQGVKEIISDVATLRTVPSAGGRHALETILLVNN
                     VEGLTLGLYRYLSLEHQLGTIKKDSSIVNDIVKASFSQDFIKQSAVTFIWIAIIYRMK
                     WRYGERAYRYIFLDAGHVAQNLYLSAEAINCGVCVIGAFSDDELNSILDLDGQEHFVI
                     YMATVGKKIR"
BASE COUNT          992 a          647 c          481 g         1045 t
ORIGIN      
        1 aggcacaggt tcctaagaat ctattgtata ccagtaaaaa aatggactct ccctttcagc
       61 tcttttaatc ttttgagcct ggttgtattt tctgaattat tacttgaaca tctaataaat
      121 taaatgagat tttctgataa atatttcaaa cgcttactct ttaacttatt attctccaaa
      181 catgaatcta tctacttttt tgttatagta ccagaaattt caaaggtatg ttgtttcatt
      241 ttgatccact tttcttcttc tatcccaaca acatttccgc aaatacatct aatttctttt
      301 ccattatgaa tactataatc ccgtttaatc ctacccttcc agcaatgtaa cagtttgccc
      361 ttgccaattt tttgatattt aaatatatta cttttacatt tgatacattt aactgtaatc
      421 atagttacct gtaaataatt gaagatagct ataactaagc gtctgagata gaatatctac
      481 tgagaaaggc acctgccacc actaaaaggg aggctatcca taggcggact tcaatagtga
      541 tgtgcaaata caatgagctg ataaaaactg ataaaagggg ggtaaagtat gaaagtgaag
      601 caaccaataa tatattaccg cgacgcatag ctctatccca aaaagaatag gctaaaaatg
      661 ttgggaaaac agccatatag agtagctcaa tacccgccct gatagtccat cgcgattctt
      721 cttgatagaa tatcctcagt atcaataaga gcagaccggt aatcaataaa aatataggca
      781 ctgcgccttc tttatctctg gcagcccact ttctggccag attggaatag agagcccagg
      841 acagcgcagc aataaaagca agtatatatg gtagactgtt gtttttaata ttttgaaaaa
      901 aactatagat tgaaagactg tgataaggat tcataccaat atagacacct gcaaaactga
      961 gtatggtgcc tggaacaatc aggaaatatg ctttcttttt taaaataggc actgaaaaaa
     1021 gcagagttag tcccggccag agataattaa taatactgac ctcaataacc tgttggttgt
     1081 taaatgatag tcctatagcc aaatataagg aaatcatata taggacaaat aaaaatccgc
     1141 atccccataa ataattgtaa ggcaagtgaa tcattttttt aatggtatgt ggtgaacgaa
     1201 taattaaata gaaccaggac aacaatccgc ctgagataaa aaccaatccg ctggtagtca
     1261 ctacacctaa ttgttctgtt aagctcctgg aaaaggcaac ggtactcccc caaaataaaa
     1321 ttgctaaaat tcctaacaaa gtactattgt ctttcttgtt tagctctttg gccaccacaa
     1381 tcaatctccg ttaccgctca tttatcttat tcctgcttta caggagataa taatttttaa
     1441 tttaaattat aatttttaat cttaccaatt gccttgatat ctaaaacggg gctaccctga
     1501 aaagcatcca atccctttac tttcagttcc aatccggata cctgcagcag ctctacatat
     1561 gttatcccga ttcgggaagt tcttctcggt gttcgagaag caaaaacacc tactctttta
     1621 ccatcactct tgttaccacg gttgaagacc gttttgatag catcaatttc atgaaaataa
     1681 aagaccacac atatctcttt ttcttcttca agcttataca taaacctctt ttgttgaggg
     1741 tataatgcta tcactgcatt cccccagggt tcgtttgatt taacaaaacc tatcggataa
     1801 attgacactg gctttacatc accaaaaata cagcggtagc agactggttt ttcatcaaca
     1861 taaaaaaatt gttcaggatt tattctcttc tgacattctg aacaaaacag ttcttctttc
     1921 aaattactgg cctcgtttct ttttaaagaa ttagttttac tcactggaaa ctgatctttt
     1981 tccagttata tgttcaggtc gtactcttag aatggttaat ttattaagat tatcttcatt
     2041 aaataccggc ttttcttctt tagttgtcgc tctttctcca tagagctgct ctacaataca
     2101 gtttagcgca tttattcttt cccttctatc aaacaggaac tctacttttc caaaaataat
     2161 tacactgctg tatctcatac tggtcttaca gacatcttct gattgattaa tttttagatc
     2221 ctgggttatt tcaatacata cccgggggtt ttcttttaat atatctattt tcaaaccttc
     2281 cggagcagag tgcaggtaaa aatattgccc atcaaatcca aaattcatgg gtaccagata
     2341 gggagcattg tccttgcaca ttgccacatt acaaaaaaga gctttcttta taatatcttt
     2401 tatttcttcc ctactgctta tttctctatc ttttcttctc atcttttaaa aatcctctct
     2461 aattatttca tactattctt atcttatctt tttacctaca gttgccatat agataacaaa
     2521 atgctcttgg ccatcaaggt ccaaaattga attcaattca tcatctgaga aagcacctat
     2581 tacacaaaca ccacaattta tggcttcggc agaaagataa aggttctgag caacatgacc
     2641 ggcatctaaa aatatataac gataagcgcg ttctccatat ctccatttca ttctgtatat
     2701 gattgcaatc cagataaaag tgactgccga ttgctttata aaatcctggc taaagcttgc
     2761 ttttacaata tcattgacta tagaactatc tttttttatc gttcccagct gatgttccag
     2821 acttaagtaa cgataaagtc caagagttaa tccttccaca ttatttacca gaagaatggt
     2881 ctccagagca tgacggcctc cagcagatgg cacggttcta agggttgcta catcagaaat
     2941 aatttctttt accccctgag tgcaccagag taaataggac agctctaaca ggttaatatt
     3001 ttctcgtttg taatttctga tgctctgtct cacattgata atagaaatta tattgtcttc
     3061 agatacttgt attttatccc agggcggtaa tgaaataatt tcccctgttt cgggataagg
     3121 cagttgcaga cctggcgagg gtgctccttt catctgatca gctac
//
LOCUS       DAOQWT010000143         3162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_32483_length_3162_cov_2.887029, whole genome shotgun sequence.
ACCESSION   DAOQWT010000143 DAOQWT010000000
VERSION     DAOQWT010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32483_length_3162_cov_2.887029"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKJ95_03645"
     CDS             complement(<1..234)
                     /locus_tag="PKJ95_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65373.1"
                     /translation="MLFIFDMGGVVANHTNVIPSIAEHLEINEKKFFKLAGQNWINLL
                     CGKISSQQFWKQFSEEIKQNIKTDLFKKYFYPTI"
     gene            complement(255..1130)
                     /locus_tag="PKJ95_03650"
     CDS             complement(255..1130)
                     /locus_tag="PKJ95_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNR65374.1"
                     /translation="MQKKWQKKTKNSNISSIIINSFIWLFLIGTAMFVLAPLVFMFTA
                     SVMPASDVMKMPFPWIPKGIYWQNYWQGIRGNDGSFIYIRNILNSFIVASSVTVTTVI
                     LSAITGFGLAKYRFWGRNAVFMLIMATFMIPFEAIMIPLYLVVTKLGLQNSYQGLIIP
                     FLVNAFGIFLMRQYFITFPDEILDAARIDGASEMMIFRTIIVPNSIPAMATLAVLTFK
                     SQWDNLLWPLLIVQSEEMKTIPQYIVRFISEKHTDEGALMAVASIASIPILILFLRLS
                     KYFIGGAALFSSRKG"
     gene            complement(1141..2052)
                     /locus_tag="PKJ95_03655"
     CDS             complement(1141..2052)
                     /locus_tag="PKJ95_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNR65375.1"
                     /translation="MKYPLFKSGIEQKKSRWGLFFVIPSILFFSLFSFYPIFTALYTS
                     FFRKKLLSLQPPDFIALQNYAYLLKSPDFWNSIRATAVFTIGTFIPIVILSLILANFI
                     VSRKRFQQFFQMAYYSPAVLSSVVVAVIWLLIFDPRGLGNQVLNFLFNSPGVDHKFLA
                     NSIMVQISTMVVYSWKYLGYFTIIFIVGLTTIPQSTHEAALIDGASRWQDFYYITLPL
                     LKPTTVLVSVMSMLQCLKTFSTQYLFTQGGAPMAPINVITLNIYHTAIRDHRIGRASA
                     MSVILFFIMLIFTWLQFRVSRSEEVSY"
     gene            complement(2119..>3162)
                     /locus_tag="PKJ95_03660"
     CDS             complement(2119..>3162)
                     /locus_tag="PKJ95_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNR65376.1"
                     /translation="QGPDMFNLQIEDEYAYIVNNRLAPIDYKAAGYDSLEAVYQDYMP
                     GTLDSVTFENNLYGLPMELTNWCIYLNKRVFWDAGLDPDKDYPKTWEEMVEVSEKIVI
                     RDGEIITRRGFDFRYPYYLVAMVPMAEQLGGKLISDDGKKAIINDEAWLKFLTYMQQW
                     GPNGKNLGSPTYTPARNLFNKDNNDIAMCQSGLYQQGRIRADNPEFYDSGDWAVIPYP
                     LFEDAVRDVASAYYGHYFVVNQQKPKENQEITWKFISYMLNYPEEYLVKVGLIQPKVR
                     LMESDEFKNMPYSDVFRQDMERGHIVYYGENSAIIQQHIREAVESVMLAGRTPEAALQ
                     VLKLKVQEVLEEE"
BASE COUNT         1062 a          635 c          540 g          925 t
ORIGIN      
        1 gatggtagga taaaaatatt ttttaaataa atctgtttta atattctgtt ttatttcctc
       61 agaaaattgt ttccagaatt gttgactact tattttacca caaagtaaat ttatccaatt
      121 ctgcccggct aatttaaaaa atttcttctc gtttatttct aaatgctcag caatagatgg
      181 aattacattg gtgtggttgg ccacaacacc acccatatca aaaataaata acatgttcaa
      241 gaaacctctc tttcctaacc tttgcgagag gaaaatagag cagcgcctcc gataaaatat
      301 ttcgatagtc ttaaaaacaa aatcaaaata ggaatactag caatggaagc aactgccatt
      361 aaggcacctt catcagtatg tttttcagaa atgaatctga caatatattg aggaattgtc
      421 ttcatctctt cactttgaac aatcagtaaa ggccacaata gattatccca ttgtgactta
      481 aatgtcagca ctgccagcgt tgccattgct ggtatactat ttggaacaat gatagttcta
      541 aaaatcatca tctcgctggc accatcaatc cgtgccgcat caagtatttc atcagggaag
      601 gtaataaaat attgtctcat tagaaatata ccaaaggcat tcactaaaaa aggaataatc
      661 aatccctgat aggaattctg taaacctaat ttggttacta ccagataaag gggaatcata
      721 attgcttcaa agggaatcat aaaagtggcc ataatcagca taaataccgc attccttccc
      781 cagaaacgat atttagctaa tccaaaaccg gttattgctg aaagtatgac ggtggtgacc
      841 gttacagagc tggcaacaat aaatgagttt aagatgtttc taatataaat aaaactacca
      901 tcattacctc ttataccctg ccagtaattt tgccagtaaa tacctttagg gatccaggga
      961 aaaggcattt tcatcacatc actggctggc attacagatg ctgtaaacat gaagactaaa
     1021 ggagctaata caaacatagc cgtcccgatt aaaaataacc agataaagct attaatgatg
     1081 atagatgaaa tattactgtt tttagttttt ttttgccatt ttttctgcat aatgtaatcc
     1141 ttagtaacta acttcttcag aacgagaaac tctaaactgc aaccaggtaa agataagcat
     1201 tatgaagaat aatatgacac tcatggcact cgctctgccg attcggtgat cccgaattgc
     1261 agtatgatag atatttaggg taattacatt aataggagcc atgggggctc cgccctgagt
     1321 aaataaatac tgggtactga aagtctttaa acattgcagc atagacatca ccgacaccag
     1381 aacagtagtt ggttttaaga gagggagagt aatatagtaa aaatcctgcc agcgactggc
     1441 accgtcaatg agagccgctt catgggttga ctgggggata gtggtcaaac caacaataaa
     1501 aataatggta aaatatccca gatatttcca gctataaaca accatggtgg atatctgtac
     1561 cattatggaa ttggccaaaa atttatgatc aacacccggt gaattaaata agaaattaag
     1621 aacttggtta cccagtcccc tgggatcgaa tatcaagagc catattacag ctaccacaac
     1681 cgaggatagc acagcaggag aataataagc catttgaaaa aattgttgaa atctctttct
     1741 gctgacaata aaattagcca ggattaaact aagtattaca atagggataa aagttccaat
     1801 agtgaaaaca gccgttgctc taatggagtt ccagaaatca ggcgacttta atagataggc
     1861 atagttttgt aaagcaataa aatcaggtgg ttgtaaagaa agtaactttt tccggaagaa
     1921 gctggtataa agtgcggtaa aaataggata aaaactaaac aaagagaaaa aaagaataga
     1981 aggaataaca aaaaacaatc cccaccttga ttttttttgt tctattccgg atttaaataa
     2041 agggtatttc atatattttg accttcctta ataaaacaaa aagggctagg cagaaaccta
     2101 gcccctcagt cattactatt attcttcttc caaaacttcc tgaactttca atttcaatac
     2161 ctgtagagcg gcttcaggtg tcctgccggc aagcatcact gattctactg cttcacggat
     2221 atgttgctga atgatggcgc tgttttcacc atagtaaaca atatgacctc tctccatatc
     2281 ctgtctgaat acatcagaat aaggcatatt cttaaattca tctgactcca ttagccttac
     2341 tttgggttga atcaagccaa ctttgaccag gtattcttca ggataattta acatatagct
     2401 gataaatttc caggttattt cctgattctc ttttggcttt tgctgattga ctacaaaata
     2461 atgtccatag taggcgcttg ccacatcccg aacagcatct tcaaatagcg gataaggaat
     2521 tactgcccaa tcaccactat cataaaattc aggattatcg gcacgtatcc ttccttgctg
     2581 gtaaagtccg gattggcaca tggctatatc attattatct ttattgaaca aatttctggc
     2641 aggggtataa gtaggggagc ctaagttttt tccattggga ccccattgct gcatataagt
     2701 caaaaatttt aaccatgcct catcattaat aatggccttt ttcccgtcat cgctaattaa
     2761 ttttccacct aactgttctg ccatgggcac cattgctacc agataatagg gatatctgaa
     2821 atcaaatccc ctccgggtaa taatttctcc atcccttatg acaatctttt cagatacttc
     2881 taccatctct tcccaggttt taggataatc tttatcggga tccaggccag catcccaaaa
     2941 aaccctctta ttcaaataaa tacaccagtt agtcaattcc ataggcaatc catacaaatt
     3001 gttctcgaag gtaaccgaat ctagtgttcc cggcatataa tcttggtaaa ccgcctccag
     3061 actatcatag ccagcagctt tataatcaat gggagccagc cggttattca caatataggc
     3121 atattcatct tcaatctgca aattaaacat atccggcccc tg
//
LOCUS       DAOQWT010000144         3155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_32587_length_3155_cov_6.015484, whole genome shotgun sequence.
ACCESSION   DAOQWT010000144 DAOQWT010000000
VERSION     DAOQWT010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32587_length_3155_cov_6.015484"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(426..1655)
                     /locus_tag="PKJ95_03665"
     CDS             complement(426..1655)
                     /locus_tag="PKJ95_03665"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HNR65377.1"
                     /translation="MEKTLFVGCDVSSTDFVVCFMNHQGDELGYKTYSNNLPGAREFE
                     ALLLKMMGQYSFISLKIATEATSFYDLHLVEFLSSSVSLEKFQTQIFQLNPKIVSNFK
                     RAYIDKDKTDINDSFVIADRLRFGRISEPYENNKPYLPLRRLTRYRFHLVETIAREKT
                     YFFTHLYLKYSNFSLENPFTSVFGTTSLSIITEFSPEELAQMTVEELMDFIVKKGRNH
                     FSEPQKIVKAIKSMVSNSYRLKPGLSSSVDIILTTCLHTIKTLEHSLKDIDKAIEREF
                     KAFPNTLQSIKGIGPVYSAALFSEIGDVNKYDNQAALAKQAGLTWRKFQSGNFNAQET
                     TMTKTGNKYLRYYLVQAANMLRVHNKEYKDFYSKKFKEVNKHQHKRAIVLTARKFVRL
                     LYALLKKGQLYLHNYQQ"
     gene            2698..2970
                     /locus_tag="PKJ95_03670"
     CDS             2698..2970
                     /locus_tag="PKJ95_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR65378.1"
                     /translation="MRTWKEVKKDLLKDPEFAREVENLRPEYELISQIIQLRIEQKIT
                     QEELAKRTGIKQSNISRLESGSYNPSLQFLKKIAKGLGKDLSISFK"
BASE COUNT         1091 a          498 c          580 g          986 t
ORIGIN      
        1 ataattcttt tcatattctt ttttcccttt attcctttta taaagtgatt gtttccgaag
       61 aaaattaact gaaaatataa tcaatgatac aggagaaaaa aaatatatat agaattatct
      121 ttgtaaatag tgtctgttat aagacaggca atattatccc ggagaaaaag gaataatatc
      181 tataagtggt gcggtaatat gtcaaggggt aaaaagaaaa aaataccccc ttatgaaaaa
      241 taattattga agtgagcata tattttacat aaatccttta aaaatcggat accatcagga
      301 agtaaaagta atgttaaaaa aaagggcata tgtataagga agtaatgttt ttgctctcat
      361 caataagttc aagataaaag ttgtccatta tgtaaaaatg aaccttgaca attgaataaa
      421 tagtgctact gctggtaatt gtgtaaatag agctgccctt ttttaagaag ggcatagaga
      481 agtctaacga attttcgagc agttagaaca atggctcttt tatgctgatg tttattaacc
      541 tccttgaatt tcttgctgta aaaatcttta tattctttgt tgtgcactct caacatatta
      601 gcagcctgaa caagataata tctcaggtat ttattacctg ttttagtcat agtagtttcc
      661 tgagcattga aattacctga ttgaaatttc ctccaggtaa gaccggcctg tttagcaaga
      721 gcagcttgat tgtcatattt atttacatca ccgatttcag aaaagagagc agcgctgtaa
      781 accgggccaa tccctttaat agactgtaga gtgtttggaa atgctttaaa ctctctttca
      841 atagctttat caatgtcttt aagagagtgt tccagagttt taatagtatg aagacaggta
      901 gtaagaatga tgtcgacaga agaagaaagt cctggtttaa gcctgtaaga attactaacc
      961 atagatttga tagcctttac aattttttgt ggctcactaa aatggtttct acccttttta
     1021 acgatgaaat ccataagctc ttcaacagtc atctgtgcta gttcttcagg agagaattca
     1081 gtgattatag aaagtgaagt agtgccaaaa acagaagtga aaggattttc cagggagaaa
     1141 ttggagtatt tgaggtaaag gtgggtaaag aaataggttt tttctctggc aatagtttct
     1201 accagatgaa atctgtatct ggtgagtctt ctaagtggaa ggtatggttt attgttttca
     1261 taaggttctg aaattcttcc gaacctgagt ctgtcagcaa taacaaaaga gtcatttata
     1321 tctgttttat ctttgtcaat ataggctctt ttgaagttag aaacaatctt ggggttaagt
     1381 tgaaatatct gtgtctgaaa tttctcaaga gatacacttg atgaaagaaa ttctaccaga
     1441 tgaaggtcat aaaaagaagt agcttctgta gctattttta aactgataaa gctgtattgc
     1501 cccatcattt taagaagtaa ggcttcaaat tctctggctc cgggaagatt attagaatat
     1561 gttttatagc ctaattcatc tccctgatgg ttcataaagc aaacaacaaa atctgtggaa
     1621 ctgacatcac agccgacaaa gagggttttt tccataatat cccaaccttt cttttagaga
     1681 gaattaataa aaactaccag ggaacatccg tttttcttcg gagattttcc ccatagtgtt
     1741 acttcaggta taagcctgaa tttgactgga aaataaattg aacctgggtt aagggggctt
     1801 cctcaggttt tcggatcaga cagcctcgtt tattagaact aatcaaagtg taatagtgca
     1861 gaccatataa ttacttacag ctgtaattaa aaatggtttt cacaatgatt gaacagggtg
     1921 cggttagaat tcccatccgt tcccaaggta aacacactta agaaagaagt cactcttagt
     1981 aagagtgcaa ctgaggtaaa agaatttacc tcttataccc gaaaagtata atacataaag
     2041 ttacttaacc tgtaaagatg tctactggat agaatttata tacccaaaac ctgtagtata
     2101 tctatacaaa taaaaatgac tctttatgaa ctttatttta aacaatcgga tattaacagg
     2161 cagccaacta ataaaagact gctttaataa aaattgtaaa actctatact tttgcacttt
     2221 tttaataaag ccttatttta ccctgcagag cacccgcgag ggtgctccta cgggataacg
     2281 attttttaaa attttgcaaa gtatagtaaa actataaaga aaaaataagg tgattgacat
     2341 aggtctatca gggccggata cgacaacctc acttattaga actaaccatc aatagagata
     2401 cattactgat ggtaatcagg atatattctc taaaatatgg ttgaacaggg tgcgagttag
     2461 aatttatatc caggcccgag ggaaacaccc tttttaaaga agacaccctt aattctataa
     2521 ggggctacag gagggaatag agttttcctc tcatacaact gtatgtattt agacctataa
     2581 aatttcatca attttaattt aggtattatt aattaagtat gaatttacgg ttttggattt
     2641 tataaaatcc ttaaattccg ttttcatata ttactattat acgaggaggg ttaaagaatg
     2701 agaacatgga aagaagttaa gaaggatctc ctgaaagatc ccgagtttgc aagagaggta
     2761 gagaatttaa gacctgaata cgaattaata agtcagatta tacagctccg tatagagcag
     2821 aaaatcacac aggaagaact ggcaaagaga acaggtataa aacagagtaa tataagtcgt
     2881 ctggaaagtg ggagctataa tccttcactt cagtttttga aaaaaatagc gaaaggcctc
     2941 gggaaggatc tgtctatatc ttttaaataa gtgtataaat cgaagccgaa gatggatgag
     3001 ccggatattt tcttctgctc tgtcctggaa atccggctca tcgctgcaaa caattcaaat
     3061 tctaattaga aaatttcatt actgaggcct taatcacgga cggcccatct ttccaatcat
     3121 cataacctac ataaggtatt ccattggata cagac
//
LOCUS       DAOQWT010000145         3135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_32897_length_3135_cov_2.771429, whole genome shotgun sequence.
ACCESSION   DAOQWT010000145 DAOQWT010000000
VERSION     DAOQWT010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32897_length_3135_cov_2.771429"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            505..1869
                     /gene="dnaA"
                     /locus_tag="PKJ95_03675"
     CDS             505..1869
                     /gene="dnaA"
                     /locus_tag="PKJ95_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNR65379.1"
                     /translation="MDYNQLNKKWEDALQIIKERISVQVYNTWFSEVKYIDFKNNQLL
                     LTVPNSFCKNWLEKNYYQLIKDIITEQFDFKEEIIIKFTISSTPDQGMGKNKKEKGKE
                     DVLQKRYEETGLNIKYTFDNFVVGDSNRFANAACMAVAQSPAKSYNPLFVYGGVGLGK
                     THLVNGIGNYIINRNSFQSVNIAYVSSEKFTNELINAIKDDTNEVFREKYRNFDILLI
                     DDIQFLAGKERTQEEFFHTFNTLYNASKQIVITSDRAPKEIINLENRLISRFEWGLIV
                     DIQPPDFETRVAILQKKAEDLQLKLSDEIINYMANKIPSNIRQLEGALNKLNAYITLT
                     TQKEVDFTLAQQILKDFIAIEEKDISIQTIQKQTAEFFALKPGMLLSPKRTKNIVTAR
                     HIAMYLSRELTDYSLPMIGDSFGGKDHTTVIHSYRKIKEQLKTDKKLKLSIDRLINNI
                     KKSL"
     gene            2251..>3135
                     /gene="dnaN"
                     /locus_tag="PKJ95_03680"
     CDS             2251..>3135
                     /gene="dnaN"
                     /locus_tag="PKJ95_03680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNR65380.1"
                     /translation="MKVICSQKSLVNGIQIVQKAISTKLGLPIYSGILFEVTSDNKIH
                     LFSTDLEIGIDCYLPAQIIEQGTVVIPSKIFGELIRKFPEGNIEIEVDKNNIIYIKEE
                     DLSNYKILGFSADDFSPFPEIQSNVKIKLTHKILKDTIQEVIFASSRDENRSFLNGIL
                     LKRVEKGLEVAATDSHRLALKKIKLNEKNKIETNNQFEVIIPQRALSELFKLLNFEDD
                     SIVEIDIGEKQVIFVLNSEGEKNKIRLYSRIIDGQYPDYHQIIPHQFRTSVKIDTEEF
                     RQRMERIILFVKEDLNTIR"
BASE COUNT         1189 a          392 c          455 g         1099 t
ORIGIN      
        1 tagcccctta tttttgttat tatattaaca gaaaaattat gattaaaaat taaagattgt
       61 cttttattca aaactgaatt aatattgcag agcaatttcc cttattcaag aaactttctt
      121 ctggaaatat gataactgtt aaatattatg tagtttttag ataacaccag cgatataatt
      181 atttttttat tattgtgata atatgtcctg ttttttactc atataaattt ttaatttatt
      241 tttccacagt tgttaataag ttgtgaatta aatcaaaacc tttagtatta cctgattttt
      301 tcatagctct tttttaaaaa aggctgaagg gtattttcct taaagctata ttacctttag
      361 ttaataaata aagtgattgt tttccacaat ttattgttaa taatgttaat aacttttttg
      421 tttttatgat acttccgtca actttttaat aaattagatt ataattgatt aaatatttac
      481 cgattaacga ttagaaagaa tgccatggac tataatcaat tgaacaaaaa atgggaagat
      541 gccttacaga taattaaaga aaggataagt gtccaggttt acaacacctg gttttctgaa
      601 gttaaatata ttgactttaa aaataatcaa ttattgttaa ccgtacctaa tagtttttgt
      661 aaaaactggc tggaaaaaaa ttattatcag cttattaaag atattattac cgagcagttt
      721 gattttaaag aagagattat aataaagttt actatttctt caactccgga tcaaggtatg
      781 ggtaaaaaca aaaaagagaa ggggaaagaa gatgttcttc agaaaagata tgaagaaacg
      841 ggtttaaaca taaaatatac ttttgacaat tttgtggtag gagatagtaa tcgcttcgct
      901 aatgcagcct gtatggccgt tgcccaatct cccgcaaaat catataaccc cctatttgta
      961 tatggaggag tcggtttagg caaaacacac ctggttaatg gtattggaaa ctatatcatt
     1021 aacaggaatt cctttcaatc tgtcaatatt gcctatgtct cttctgaaaa attcaccaat
     1081 gaattaatta atgctattaa agacgatact aatgaggtat tcagagaaaa atatcgtaat
     1141 tttgatatct tgcttattga tgatatccaa tttttagctg gtaaagaaag gactcaggaa
     1201 gaatttttcc atactttcaa tacactctac aatgccagta aacagattgt cattacctcc
     1261 gaccgtgcac caaaggagat tattaattta gaaaatcgtc ttatttcaag gtttgaatgg
     1321 ggattaattg ttgatattca acctcctgat tttgagacga gagttgctat attacaaaaa
     1381 aaagcagaag atttacagtt aaaattatca gatgaaatca ttaattacat ggctaataaa
     1441 attccttcca atattcggca gttagaaggg gccttaaata aattaaatgc ttatataact
     1501 ctaactactc aaaaagaagt ggattttacc ctggctcaac agatattaaa agattttatc
     1561 gctattgaag aaaaagatat atctattcag accattcaaa aacagacagc agaatttttt
     1621 gctcttaaac caggaatgtt gctatctcca aaaagaacta aaaatattgt aacagctcgt
     1681 catattgcta tgtatctatc cagagaatta accgattatt ctctccctat gattggagac
     1741 tcctttggag gaaaggatca taccactgta atacattcct atagaaaaat aaaagaacaa
     1801 ttaaagacag ataaaaaact taaattaagt atagataggc taattaataa tattaaaaaa
     1861 agtctataat ttataaatta tttttcaact ttaaaagtat attgtttatt acattacttt
     1921 atagttagtc attttcttat attttaattt ttcttgttgt tttctagaat acaaatgtta
     1981 atactattga aaaaataggg tagttattaa gaataatatc cacctgtttt tcgattattt
     2041 cataaaggat tattaatatt attaacataa ttaacactat taatactatt aatactatat
     2101 tttctattta attataatta tctttaaata gcaaatattt aaaatattat tactttttta
     2161 aattttgtga attttaatta tataaaaact actgttatat ttaaaatttg atatgcttaa
     2221 aatatttaaa attaataata ggagaagata atgaaagtta tatgttctca aaaaagttta
     2281 gtaaatggga ttcaaatagt acaaaaagct atatccacca aattaggttt accaatatat
     2341 agtggaattc tttttgaagt taccagtgat aataaaatac atttgttttc aactgattta
     2401 gagataggga ttgactgtta tctcccggct cagattattg aacagggtac tgttgttatt
     2461 cccagtaaaa tttttggtga attaatcaga aaatttccag aaggaaacat tgaaatagaa
     2521 gtcgataaaa ataatataat ctacattaag gaagaggatt tatcaaacta taagattctt
     2581 ggtttttctg ctgatgattt ttctcccttt ccggaaattc agagcaatgt aaaaataaaa
     2641 ttaactcata aaattttaaa agataccatt caagaggtta tttttgcttc ttcaagagat
     2701 gagaatagat cctttttgaa tggaatatta ttaaaaagag tagaaaaagg gttagaagtt
     2761 gctgcaactg atagtcatag attagcttta aaaaagatta aattaaatga gaagaataaa
     2821 attgaaacta ataaccaatt tgaagttatt attcctcaaa gagctttatc tgaattattt
     2881 aaactactca actttgaaga tgatagcata gtagagatag atataggaga aaagcaggtt
     2941 atatttgttt taaactcaga aggagagaag aataaaataa gactatactc gcggataatt
     3001 gatgggcaat acccggatta tcatcagatt ataccgcatc aatttagaac ttctgtgaag
     3061 attgacactg aggaattcag acaaagaatg gaaagaatta ttttatttgt caaagaagat
     3121 ttaaatacca ttaga
//
LOCUS       DAOQWT010000146         3113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_33244_length_3113_cov_3.926095, whole genome shotgun sequence.
ACCESSION   DAOQWT010000146 DAOQWT010000000
VERSION     DAOQWT010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33244_length_3113_cov_3.926095"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1862
                     /locus_tag="PKJ95_03685"
     CDS             210..1862
                     /locus_tag="PKJ95_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNR65381.1"
                     /translation="MLRKNNFSQYFIALLTVLIVLTFLSTIGWANEENGTGSYGILSL
                     IPPILAIILCIVLKEALISLIIAVFVGTTILNSGKFWIGIEETANILVAQVADSWNAS
                     IILFFFVVGGLMGMIFFSGGGQAFLESISKKANNSKMAQIFAWLGGIVIFIDDYANSA
                     FIGNLFRPLSDKYYVSREKLAYIVDSTAAPVSSIFLISTWIGYQVGLIGESLPEGINV
                     SPYYLWLNSIPYSFYSILAILMVGIIALTGRDFGPMLKAEYRARTTHQLWAEGARPLA
                     GTSELKIAKNASTKPINLWLPIILLVVLSFYFMWTSGGGSSAESFSQAISDADSMFSI
                     FLATLIAFFVAIIMYLVQRIATVAEIMDSFLNGARMMVYATLILISAWAIKAVCDELG
                     TAPYIVNALEGVLSPMILPILTFLVSCFIAICTGTSWGTMGIVVPIVIPLGISVGAPL
                     PIVISSVLTGAVMGDHCSPISDTTVMSSTFAGSDHIDHVRTQFPYSLTATFVTVICYL
                     LAGMGIPVIVVLLAGVLLLYLLIFILSSLSAKQLGISFPLEK"
     gene            2116..>3113
                     /locus_tag="PKJ95_03690"
     CDS             2116..>3113
                     /locus_tag="PKJ95_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HNR65382.1"
                     /translation="MIRQNILIMGAAGRDFHNFNVFFRDKEAYNVVAFTATQIPDIAG
                     RKYPAQLSGLLYPDGIPIFPEEDLPQLIEKYQVHQVVLAYSDLPHQYVMNKASLVLAK
                     GADFRLMGPNNTMLQAKVPLISVCAVRTGSGKSQTTRKVTRILKEMGKKVVVIRHPMP
                     YGDLSKQIWQRFEKYEDLDRYQCTIEEREEYEPHIDLGNIVYAGVDYGEILKRAEQEA
                     DVIVWDGGNNDIPFYKTDLHIVVADPHRAGNEMSYYPGQVNLRMADVVVINKIDTAEP
                     DNIILLRNNIFNLAPQAILVEAASPIMVEHAEMIRGKRVMVVEDGPTLTHGEMQFGA"
BASE COUNT          934 a          530 c          637 g         1012 t
ORIGIN      
        1 aaagaatgct taatatttat ttagggcaat cggtgagcct ggcgcttata gattgtcctt
       61 ttttaataag attattaagc aaagtgagaa gatgaaaaga acatatattt aaatattcag
      121 gaaaggaggg gaatattatc acaatagaaa agtattaaaa aagttttaag gtaataattg
      181 aaaatatggt aaaggagtgt tttaaaaaaa tgttaaggaa aaacaatttt agtcaatatt
      241 ttattgccct cttaactgta cttatcgttt tgacattttt atcaacaata ggatgggcta
      301 atgaagagaa tggtacaggt agttatggaa ttttgtcctt aatacctccc atcttagcca
      361 tcatactttg tattgtttta aaagaagcct taatttccct gattattgcc gtttttgtcg
      421 gcactacaat tctaaatagt ggtaagtttt ggattggtat agaagagaca gccaatatat
      481 tagttgctca ggtagctgat tcctggaatg ccagtattat cctgttcttc tttgtagtgg
      541 gcggtttgat ggggatgata ttcttttccg gtggtgggca ggctttttta gaatcgatca
      601 gtaaaaaggc taacaattcg aagatggcgc agatctttgc ctggctgggt ggtatcgtaa
      661 tatttataga tgattatgcc aattcagcct ttattgggaa cctatttcgt cccctatctg
      721 ataagtatta tgtttccaga gaaaagttag cctatatcgt agattcaact gctgctccgg
      781 tttcttccat atttctgatt tcaacctgga taggttatca ggtaggctta ataggagaat
      841 ctcttcctga aggaattaat gtctcaccct attatctatg gttaaacagt attccctata
      901 gcttttattc tattttagcc attcttatgg taggaattat tgcccttacc ggaagagact
      961 tcgggccgat gctgaaagca gaatacagag ccagaactac acatcaatta tgggctgaag
     1021 gagccagacc tctggctggt acctcagaat taaaaatagc caaaaatgct tccacaaaac
     1081 caataaatct atggctacct ataatattgc tagtagtact gtccttctat tttatgtgga
     1141 ccagtggtgg tggtagttct gctgaatcat tttctcaggc aatctccgat gctgattcca
     1201 tgttctccat attcttagcc acccttattg ccttttttgt agctataatt atgtatttag
     1261 tgcaaagaat tgccactgtg gctgaaataa tggattcctt tctcaatggt gccaggatga
     1321 tggtctatgc cacattaatt ttaatctcag catgggccat taaagcggta tgtgatgaat
     1381 taggcactgc accatatatt gtaaatgcac tggaaggtgt tttatcgcca atgattttac
     1441 ctattttaac tttcctggtc tcatgtttta ttgctatatg taccggtacc tcctggggta
     1501 cgatgggcat tgttgttccc attgtaattc ctcttggaat tagtgtgggt gcacctcttc
     1561 ccattgtcat ttccagtgtt ttaaccggtg cagtaatggg tgaccattgc agtcccattt
     1621 cggataccac tgttatgtct tcaacttttg ccggttcaga ccacatagac cacgttagaa
     1681 cccaattccc ttactccctt actgctacct ttgttacagt aatttgctat cttctggcag
     1741 gaatgggaat accggttatt gtagttttat tggcaggagt tttactactg tacctgttaa
     1801 tatttatttt gtcttctcta tctgcaaaac aattgggtat ctccttccct ctggaaaaat
     1861 agaaggcaag taataagtta aaaaaataac tattataact gtaactaata ctgggcaatc
     1921 tgtcctgtca ttaatgcctt aaataagaaa taggacagga tggattgcct tttattaatt
     1981 tttaataaaa tttctttttt ctaagaattg ttatgcctct acactaaatc agatcaaaac
     2041 tatttaaaga aaattgattt attgagccaa acataataaa attaaaatat aattatagac
     2101 aaaggagtga tttttttgat aagacaaaat atacttatta tgggagcagc tggcagagat
     2161 tttcataatt tcaatgtttt ttttagggat aaagaagctt ataatgtggt tgcctttact
     2221 gctactcaga ttcctgatat tgccggtcga aaatatccag ctcaattgag cggtctgcta
     2281 taccctgatg gtataccaat tttcccagag gaagacctgc cgcaattaat tgaaaagtat
     2341 caggttcacc aggtcgtatt agcctacagc gacttgccgc atcaatatgt aatgaataaa
     2401 gcatctctgg ttttagcaaa aggtgctgat ttcagattga tgggaccgaa taacactatg
     2461 ttgcaggcta aggtgccact catatctgtt tgcgcagttc gtaccggaag cggtaaaagt
     2521 cagactaccc gtaaagtaac cagaatatta aaagaaatgg gtaagaaagt ggtggtaatc
     2581 agacatccta tgccttatgg ggatttaagc aaacagatct ggcaacgttt tgaaaaatat
     2641 gaggaccttg acagatatca atgtactata gaagagaggg aagaatacga gccccatatt
     2701 gatttgggta acattgtcta tgccggagtc gactatggtg aaattttaaa aagagcggaa
     2761 caggaagcag acgtcattgt atgggatggt ggaaacaatg atattccttt ttacaaaaca
     2821 gatttacata ttgttgttgc cgaccctcac cgggctggaa acgaaatgag ctattatcca
     2881 ggccaggtta atttaagaat ggcagatgtg gtagtgataa ataagattga tactgcagaa
     2941 ccggataata taattctact gaggaataat atttttaatt tggcacctca agcaatactg
     3001 gtagaagctg cctctcctat tatggttgag catgcagaaa tgattagagg caagagggta
     3061 atggtagtgg aagatggtcc taccttaact catggagaga tgcagtttgg cgc
//
LOCUS       DAOQWT010000147         3108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_33336_length_3108_cov_3.202096, whole genome shotgun sequence.
ACCESSION   DAOQWT010000147 DAOQWT010000000
VERSION     DAOQWT010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33336_length_3108_cov_3.202096"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="PKJ95_03695"
     CDS             complement(<1..1719)
                     /locus_tag="PKJ95_03695"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNR65383.1"
                     /translation="MKSKMRKLIRVILGLTMMLSIAIGVVNSVIADEISDITTPFELT
                     KNTARTEIEAAIAAGKAAGATVAIMDGREFVYAEGFGMADREKNIPVEHNTVFNIGSV
                     SKVFAATSIMMLVDDGKVSLDEPIITYLPEFRMADERYRDITVRMLLTHSSGLPGTTA
                     WNNFGSEYNQNIYSELLAVLSRSTLKHKPGELAAYCNDGFTLAEMIVAEVAGMGYGDF
                     LIERIFKPLAMDHTCPGFNRLPEGLTPAKYYRADGKGEPLEIGSLLASGGLSSTAEDL
                     CRFATTFSGSKVILSSQSLGVMLKRQPNEFQGKLRGDGFPFGLGWDFAELTPYPEKDI
                     VLFGKTGGTGHYNAMLYTVPSQQISVAVIGSGPQCDSPGIAFKVISAYLKEKGLITEE
                     ETEDKEPFEAESFPTNLLAYEGYYDSVGITMRIALDDEKGILTCYLVDGNDESALYSA
                     IYNDGFFYDDAGNAYYFITVDETNYFVAYSPLFDFHNIKAEKIEAIAHPLELSVPIDG
                     KIWLRRNAKAAEAAMLTEGHIISSMLISDLPGYIDFDGLKAVKSPDYAGMPVKSMRDM
                     SELRLYK"
     regulatory      complement(2131..2221)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          920 a          709 c          503 g          976 t
ORIGIN      
        1 tttgtagagc ctcagttcgc tcatatctct catagatttt accggcatgc cggcataatc
       61 aggtgatttc acagctttta atccatcaaa atctatgtat ccaggtaaat ccgagattag
      121 catagaagaa ataatgtgac cctctgtaag cattgccgct tcagctgctt ttgcgtttcg
      181 cctgagccat attttaccat ctataggcac tgataattcc agtggatgtg caatggcttc
      241 aatcttctca gccttaatgt tatggaaatc aaataatggt gagtatgcaa caaagtaatt
      301 tgtctcatcc acggtaataa agtaataagc gtttccagca tcatcataga aaaacccatc
      361 attatatatt gcagaataca gggcagactc atcattacca tctaccagat agcaggttaa
      421 tatacctttt tcatcatcga gggcaatccg catggtgata ccaacgctat cataataacc
      481 ttcatatgct aacagattgg ttggaaatga ttctgcttca aacggttcct tatcttccgt
      541 ctcctcttca gtaatgagac ccttctcttt aagataagca gatattactt taaaggcaat
      601 acccggggaa tcacattgcg gaccactgcc gattacagct actgagattt gttgagaggg
      661 taccgtgtag agcatagcgt tatagtgacc tgtgccaccg gttttaccaa aaagaacgat
      721 atccttttca ggatatgggg taagttcggc aaaatcccaa ccaagaccaa aggggaaacc
      781 atctcctcgc aatttacctt gaaactcatt tggctgcctt ttcaacatca cccccaatga
      841 ctgagaagac aggataactt tgctgccgga aaaggtagta gcaaaacgac ataagtcctc
      901 cgcagtggat gataatccac cactggccag tagtgagcct atctccaagg gctcaccttt
      961 accatcagca cgatagtatt tagccggggt aagcccttcg ggaagccggt tgaaacccgg
     1021 acaggtgtgg tccatagcca atggtttaaa gattcgctct atcagaaaat ccccatatcc
     1081 catacctgcg acttcagcca caatcatctc tgccagtgta aaaccatcat tacagtaagc
     1141 cgctaattct cccggtttgt gcttcaatgt ggaacgggat aacaccgcca gaagctcact
     1201 ataaatattc tggttatatt ctgagccaaa attgttccaa gcagttgtcc caggcaaacc
     1261 agatgaatgg gttagtaaca ttcttactgt gatatcccga taccgttcat ctgccattct
     1321 aaattccgga aggtaggtta taatgggttc atctaaagag actttaccat catctaccag
     1381 catcataatg gaggttgctg caaacacttt gcttaccgaa ccgatattaa aaaccgtatt
     1441 atgctcaacc ggaatattct tttcccggtc tgccatgcca aacccttccg catagacaaa
     1501 ttctctacca tccattatcg ccactgtcgc gccagccgcc ttcccggcgg caatagctgc
     1561 ctcaatctca gtgcgagctg tatttttggt cagctcaaag ggggttgtta tgtctgatat
     1621 ttcatcagca ataacggaat tgaccactcc aatagctatg gacagcatca ttgtaagccc
     1681 aagaatcact cgtattaact ttctcatctt acttttcata aaataataaa cttcccttct
     1741 attcagtttt ttatagggga cggttctctg tttgagccct tttctctgtt tttattgcaa
     1801 gcatgaagca gaaaccgtct cttgtttgta aaaaataatg ccagaggatt gtctcctggt
     1861 attaatggca atcataaagt gcaagtcata gtaaattatt aaagaacaat tatgttaaag
     1921 ttccctgctc tgtaataaga aaaaagttgt gaaacagata attgtccact ttttaaacat
     1981 actaatgtag ttttttataa tatataaata agaccatcac ctttcttatt aaaatcctta
     2041 caatatagtt atcacaaaga aattatactt ttattttcta tggtaaattt ctagttttgt
     2101 acctttgcct ttataagaat aaaaaaataa atcggcagtc tgccagccat tgtatatctc
     2161 tacatacttt aggcacataa ctttgcgccc taccctttcg gatagtttgc ctttatcaac
     2221 ttattgcaag tttctattta ttattttaat gttctatcta cattattccc tatatttttt
     2281 attttagaca atatttttca attttttaca gggtttttac aagggacggt tctctgtttg
     2341 atgagtttga tgtgtatcat gtgtatcatt ctttgacaat ctgatataca gtcaatccgg
     2401 tcaatctgaa agctaccttg gggaacaatc ctttaatttt ttaaataaga taattttata
     2461 aatatattct actctcctgt catttcgaag gagtgcaacg actgcggcaa tctcatctgc
     2521 tttccatcat cttccgtcat tgcaagacat gttgaaaaca tgtcgtgcca atctcatcca
     2581 tttctgccca catcttcctc tgttaatccc tcactttcgc ccgcatttat ttaattttta
     2641 tttgaatttt accaaatact atatacaaat gactaacgac cacttatatt tattcctgca
     2701 atgatttaac taaaaactat gaactataga cccctttcaa ttattcccac atctttattc
     2761 actaaaaacc ataaaccatt aacccctttc acttatcccc cacatctttt taaccaaata
     2821 ccatcgacca acgactatcc tattcccaca tcttttttgc aaaaaatcac cccctgtgaa
     2881 aaggtacaaa gatgaattca aacacgggtg attcttcaaa cgtggggacg gttcactgct
     2941 tgactcctgt ggatattgtc cagcagaaaa ccgtccccaa gttttcattt tacagagaac
     3001 cgtccccatg tttgaagtct accagcaatc ttttcctttt acctagaact aaaaatcatg
     3061 aaccgttaac tgaaaatgat ttagcaaaga accgtcccct gcaaaatt
//
LOCUS       DAOQWT010000148         3093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_33592_length_3093_cov_3.148453, whole genome shotgun sequence.
ACCESSION   DAOQWT010000148 DAOQWT010000000
VERSION     DAOQWT010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33592_length_3093_cov_3.148453"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..985
                     /gene="deoD"
                     /locus_tag="PKJ95_03700"
     CDS             287..985
                     /gene="deoD"
                     /locus_tag="PKJ95_03700"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNR65384.1"
                     /translation="MSVHISAEVGDIAPTVLLPGDPMRAKYITDNYLEESYCYNKVRG
                     MYGFTGRYQGKLISVQGTGMGVPSISIYLHELITQYQVRNLIRIGSCGAIQPDIKIRD
                     IILAMSASTDSSLNKIRFKGNDYSPTACYELLKKAELIAQEKFLPVKVGTILTTDMFY
                     HDDPDYWRLWASYGVLALEMETAALYTIAAKYKVKALSILTVSDSLVTQEETSSAERE
                     KTFTDMVEIALNLI"
     gene            1146..1544
                     /gene="speD"
                     /locus_tag="PKJ95_03705"
     CDS             1146..1544
                     /gene="speD"
                     /locus_tag="PKJ95_03705"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNR65385.1"
                     /translation="MDYLGNHLLVELYECDSLKLNDQNLLEKILREAVRISGATALKA
                     SFHQFAPQGVSGVIVIAESHFTIHTWPEYGYAALDIFTCGYSINNQKALDYIEKELDV
                     KTLSVTEIKRGNIHFPLKIAKKISSEVGVL"
     gene            1585..1938
                     /locus_tag="PKJ95_03710"
                     /pseudo
     CDS             1585..1938
                     /locus_tag="PKJ95_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733373.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
     gene            1953..2789
                     /gene="speE"
                     /locus_tag="PKJ95_03715"
     CDS             1953..2789
                     /gene="speE"
                     /locus_tag="PKJ95_03715"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNR65386.1"
                     /translation="MELWFGEQYGRENGRYLQIQVDNVLEKVKSPFQEIAVVNSPAFG
                     KILVNDSIVMLTEADEAGYHEMIAHVPLCVHPKVAEVLVIGGGDGGTVREILKHSDVK
                     RVDVCEIDEQVVEVCRRHFPDMANSFDNDKVHLYYQDGAQFLTVRRNQYDVVIVDSTD
                     PVGPGITLFTEQFYQSIFHSLKEDGIAVSQMESIFWHGKFIQNTFLFLNRIFPIARYY
                     FTMVPTYPSGMIGFSLCSKKYHPVEDFKPEKTEKLTNLKYYHAELHKAAFILPAFAQK
                     LI"
BASE COUNT         1000 a          454 c          636 g         1003 t
ORIGIN      
        1 atttttatat gaggcatcga tactatttca tatatatttt tgatgaggca attcgtattc
       61 ttcccatata atagagttta ctttataata aaaatgattt tgaacaattt tatattaggt
      121 attgagattt tttatgtcgg ctctgatgag taaattggga atattaccag gtatttaaaa
      181 ttaagaaaat gataatagat agtttttaag tctttctatt ataaaaatta tcaaaaatat
      241 ctggtttttt cttaaataat attgtaaaat attggaggta ttaataatga gtgttcatat
      301 tagtgcggaa gtgggtgata ttgctcctac tgtattatta ccaggtgatc ccatgcgagc
      361 taaatacatc acagataatt acctggaaga atcctattgt tacaacaagg taagaggaat
      421 gtatggtttt actggtcgat atcaggggaa attaatttca gtacagggaa ccggaatggg
      481 tgtaccctct atttctattt atctccatga attgattact caatatcagg ttcgtaattt
      541 aataaggata ggtagttgtg gtgctataca gcctgatata aagattcgag atataattct
      601 ggctatgagt gcctccactg attccagttt gaacaaaatc cgcttcaagg ggaatgatta
      661 ttctcccact gcttgttatg agctgctcaa gaaagctgag ctaattgccc aggaaaaatt
      721 tttaccggtg aaggttggta ctattctcac tacagatatg ttttaccatg atgatcctga
      781 ctactggcgt ttatgggcgt cttatggtgt tttagcacta gaaatggaaa ctgctgcttt
      841 atacactata gctgcaaaat ataaagtaaa ggccctttct attttaacag tcagtgacag
      901 cctggttacc caagaagaga ccagttctgc agaaagagag aagaccttta ctgatatggt
      961 ggaaatagct ttaaacttaa tataataaga aaaactagat actttcttga gttagaaaca
     1021 gaaaactaac catagaagat tggggaaaga ataaataatt ttaaaaaaga ttgatttttt
     1081 taaaattttc tgttatttta tgtaagtttc ttaaataatt ttaatggaaa aagagggtaa
     1141 aagaaatgga ttatttggga aatcatcttt tagtagaatt gtatgaatgt gattcactaa
     1201 aactaaacga tcagaatttg ctggaaaaaa tattaagaga ggcggtaaga atctctgggg
     1261 ctactgctct aaaggcatct tttcatcaat ttgcacccca gggcgtcagt ggtgttattg
     1321 ttattgctga atctcatttt acgattcata cctggcctga atatggttat gctgcactgg
     1381 atatcttcac gtgtggatat agcattaata atcaaaaagc tcttgattac attgagaaag
     1441 agctggatgt aaaaactcta tcagttactg aaataaaaag aggcaacatc cattttccat
     1501 taaaaatagc taaaaaaata tcttctgagg taggtgtgct ttaaaatgga aaaaataaaa
     1561 gatttgaaag aatattataa taaagagaag gcatggggtt tgctgaccag cattgattta
     1621 tattactgta atcctgaatt gattcgggac gcagaggcaa taaaacgcta tgtagaggag
     1681 ttatgccagt tgattgagat gaaaaaattt ggggatacac aggtggttaa ttttggagag
     1741 gatgagaggg tatcaggtta ttcaatgacc caattaattg agacatcttt gatttccggc
     1801 cattttgcca atctgaccaa tactgcttat atagatattt ttagttgtaa atactatgat
     1861 cctcaaacgg cagctgattt tacacagaaa ttttttcagg gtaaagagat gaagattcac
     1921 tatgttttga ggagatagtt gttttgaata agatagagct atggtttgga gagcagtatg
     1981 ggcgagaaaa cgggcgctat ttacagatac aggtagataa tgttctggaa aaagtaaaat
     2041 caccctttca ggaaatagct gtggtaaact cgcctgcttt cggtaaaatt cttgttaatg
     2101 atagtattgt tatgcttact gaggcagatg aagctgggta tcatgaaatg attgcgcatg
     2161 tgccattatg tgtccatcct aaggttgcag aagtgctggt gataggagga ggggatggag
     2221 gaactgttcg ggaaatactg aaacattctg atgttaaaag agtagatgtc tgcgaaattg
     2281 atgaacaggt tgttgaggtc tgccgcaggc attttccgga tatggcaaac agttttgata
     2341 atgataaagt acacctctat tatcaggatg gagctcaatt tcttaccgtt cggagaaatc
     2401 agtatgatgt tgtaattgtt gactctacag atcctgttgg gccgggaatt acattattta
     2461 ctgaacaatt ttatcaatcc atattccatt ccttaaaaga ggacgggatt gcggtaagtc
     2521 agatggaatc aatcttctgg catgggaagt ttatccagaa cacctttctc tttcttaaca
     2581 gaatatttcc tattgcccga tattacttta ctatggtacc cacttatccc agcgggatga
     2641 tcgggtttag cttgtgttct aaaaagtatc atcctgttga ggactttaag ccggaaaaga
     2701 cggaaaaatt gacaaattta aagtattacc atgcggagct tcataaagca gctttcattc
     2761 tacctgcttt tgctcaaaaa ctgatttaac gaagaggagg ctctcgtctt ttagatgtag
     2821 agcctccatt ttgtttgcaa taaataatgg aatatttcca tcacactttt ttgtttttgt
     2881 caaattacca taatataatt cctgttttgg tttatttatt atgacagata ttagtatttc
     2941 ctctggaata aaacagtcct gtccccatgt ccggctgggg gtcatctgct cagatataaa
     3001 atatgaaaag aaaaacagcg aactctggca tgtgattaaa ggagcagctg agcaaattga
     3061 aaattctctc aatcaggatc agataactaa cct
//
LOCUS       DAOQWT010000149         3087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_33678_length_3087_cov_2.889842, whole genome shotgun sequence.
ACCESSION   DAOQWT010000149 DAOQWT010000000
VERSION     DAOQWT010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33678_length_3087_cov_2.889842"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /gene="dnaE"
                     /locus_tag="PKJ95_03720"
     CDS             <1..1271
                     /gene="dnaE"
                     /locus_tag="PKJ95_03720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNR65387.1"
                     /translation="PYQKEPLRSLFSYAQFMEGKPRHLAVHASAMIIADQPLANLVPL
                     TLSPEGEIISQYEKESVKELGLLKIDVLGSRSLTVIQKTLQSINKTNNPINLREIPWN
                     DSLTFYHLKKGKTLGVFQIESSGMSSLLKQLSPSTIEDLIAALSLYRPGPLDSGMSEQ
                     YLKRKAGKAPIEYLHERLQPILKDTYGVILYQEQVMQVVSAIAGLSPGEADLFRRAIS
                     SRSPAVMEEQRQNFLTKSSRQGINTQEAQNIFNLIAKFAYYGFNKAHSTSYALLSYLS
                     CYLKVHYPAHYLAALLTYGMGYYDPDRYIQEARRFGILILLPDINKSQAGFSVEGKAI
                     RIGLIRIKSMGMKQLNSILKIREKDGPFLSLYDFCARTVSLRITGSVVENLIKVGAFD
                     FTGYPRSVLLNLLPLIIKETKKEVKQCQPL"
     gene            1256..1759
                     /locus_tag="PKJ95_03725"
     CDS             1256..1759
                     /locus_tag="PKJ95_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65388.1"
                     /translation="MSTLIEIKTKDNIINLLPEKAIPAHNFSPFELLKMEKEILGIYA
                     QKHPLTLYREKLTSRRKRGQILIQSHHIGQLRSGQPILIAGLLIQVRRQFTVNRQVMA
                     FLLLEDESGLFEAIVFPEAFQHYFSLLVKEELLIIKGKISDKNSEEKIIIQETKNLYS
                     YLEENRL"
     gene            1791..3017
                     /gene="dinB"
                     /locus_tag="PKJ95_03730"
     CDS             1791..3017
                     /gene="dinB"
                     /locus_tag="PKJ95_03730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNR65389.1"
                     /translation="MDMVRKNSIIHLDLDAFFASVEQRDNPRYAGKPLIVGGIFKKNG
                     ELSRRGVVCSASYQARKYGIHAGMPIWEAQQKCPGAIFTPSRIRYYQASSRHFFRICS
                     TYTPFVEPVSIDEAFLDVSSCEALFGTAEDIALKIKKRVKKELRLPVSVGIASNKFLA
                     KIATNLGKPDGFFILPEEKALDILSELPISKLWGIGQKSAQKLNRAGIFKIKQLMMMP
                     DVILQSILGENGPKLKQLAQGIDHSPVICSEEVKSIGRETTFPENITDRERLLKILLA
                     LSQNVGYTARKKGYSGKTITLKIRFAPFTTIQKSATLPRATNLDNTIFEKAKELLNSV
                     NIRQPGIRLLGVKLSSLQQGRQPRQLDLLNNQDWEEKWVQLISSVDKIREKYGSYLIQ
                     RAALLKEKKSNTNKKL"
BASE COUNT          967 a          647 c          631 g          842 t
ORIGIN      
        1 ttccctacca aaaagaacca ttgcgttctc tattttctta tgcccagttt atggaaggta
       61 aaccgcgcca tcttgcggtt catgcctctg ctatgattat agcagaccaa cctctggcca
      121 atctggtccc cttaacactc agtccggaag gagaaattat cagccagtat gaaaaagaga
      181 gtgtaaagga actcgggtta cttaagattg atgtactggg ctccagaagt ttaacagtaa
      241 ttcaaaaaac tttacaaagt ataaacaaaa ctaataaccc catcaattta agagagattc
      301 cctggaatga ttcgctgacc ttttaccatc ttaaaaaagg aaaaactctg ggcgttttcc
      361 agatagaaag ttccggtatg tcttccctgt taaaacagtt atcaccctct actatagaag
      421 atttgattgc agcactttca ctttaccgtc ctggtccact ggacagcgga atgagcgaac
      481 aatatctgaa aagaaaagcc ggtaaagcac cgattgaata cctccatgaa cgattacaac
      541 ccatattaaa agacacttat ggagttatcc tctatcagga acaggttatg caggttgtct
      601 cggctattgc cggtttaagt cccggtgagg ctgatctctt ccgcagggca atcagttcac
      661 gctcccctgc tgtaatggag gaacagcgcc agaattttct aactaaatct tcccggcagg
      721 gcatcaatac acaggaagcc caaaatattt ttaatctgat tgccaaattt gcctattacg
      781 gctttaacaa agcccatagt acttcttatg ctctactctc ttatctcagc tgttatctga
      841 aagtacacta tccggcacat tacttagcgg cattactcac ctacgggatg ggctattatg
      901 accctgaccg ctatattcag gaagcacgcc gctttggaat tttaattctg ctgcctgata
      961 tcaacaaaag tcaggctggt ttttctgtgg aagggaaagc gatccgaatt ggactgataa
     1021 ggataaagag tatgggaatg aagcagttaa atagcatttt aaagataaga gaaaaagatg
     1081 gtccttttct ttctctctac gatttttgtg ccagaactgt ttctttaagg ataacgggaa
     1141 gtgtggttga gaatttgatt aaagttggtg cctttgactt taccgggtat cccaggtctg
     1201 tattgttaaa tctgctgccg cttatcataa aggagactaa aaaggaggta aaacaatgtc
     1261 aacccttata gagataaaaa caaaagacaa catcatcaat ttgctaccgg aaaaggctat
     1321 tccagctcat aatttttccc cttttgaact gctcaagatg gaaaaagaga tactgggcat
     1381 ctacgcacaa aaacaccctt tgactcttta ccgggaaaaa ctaaccagcc gcaggaagag
     1441 aggacagatt ctcatccaga gccaccatat cgggcagtta aggtcgggac aacccattct
     1501 tattgccgga ttattaatcc aggtcagaag gcaatttacc gtaaaccgcc aggtaatggc
     1561 tttcttatta ttggaagatg agtcaggact ctttgaagct atcgtttttc ctgaggcatt
     1621 tcaacattat ttctccttac tggttaaaga ggagttactg attattaaag gcaagataag
     1681 tgacaaaaat agtgaagaaa aaataataat acaggaaaca aagaatctct attcctacct
     1741 ggaagaaaac aggctataat caggatatcc aaagaaggga tgtcagcggc atggatatgg
     1801 tcaggaaaaa ctctatcatt catctggatt tagatgcttt cttcgcttcg gtagaacaac
     1861 gggataatcc ccggtatgcc ggtaaaccac ttattgtagg tggtatattt aagaaaaacg
     1921 gggaattgtc ccgacgggga gtggtctgct ctgcttctta ccaggccaga aaatacggta
     1981 tccacgccgg tatgcccatc tgggaagctc agcaaaagtg tcccggggca atttttaccc
     2041 cttcccgcat cagatattat caagcctctt cacgacattt tttccgtata tgcagcacct
     2101 ataccccctt tgttgaaccg gtaagtattg atgaggcttt tttggatgtc agcagctgtg
     2161 aggcattgtt tggcacagca gaagatattg cccttaagat taaaaaaaga gtaaaaaagg
     2221 agctacggct tcccgtttca gtgggtattg cctccaacaa atttctggct aagattgcta
     2281 ccaacctggg aaaaccggat ggttttttta ttctgccaga ggaaaaagca ttggatattc
     2341 tatccgagct tccaatttct aaattatggg gtattggaca gaaatcagcc caaaaactca
     2401 atcgtgccgg tattttcaaa ataaaacagt taatgatgat gccagatgtt attctgcaga
     2461 gcattttagg agaaaatgga cccaagttaa aacaactggc acagggtatc gaccatagcc
     2521 cggtgatttg ttcagaagag gtaaaatcaa tcggtagaga aacaactttt cctgagaata
     2581 ttacagaccg ggagagatta ttgaaaatac ttctggcttt gagtcaaaac gtcggttaca
     2641 ctgcgcgtaa aaaaggttat tctggcaaga ccattacttt aaagatacgc tttgcccctt
     2701 ttaccaccat ccaaaaatct gctacccttc cacgagccac caatctggac aatactatct
     2761 ttgaaaaagc taaagagttg ctcaattctg ttaatatcag gcagccggga atacgtcttc
     2821 tgggtgtcaa actctcttca cttcagcagg gaagacaacc gaggcaactc gatcttctta
     2881 ataatcagga ttgggaagaa aaatgggttc aactgatttc ttcagtggat aaaatacggg
     2941 aaaaatatgg ttcttatctc atccagcgtg ctgcactttt aaaagaaaag aagagcaata
     3001 ctaataaaaa attatagatg aatcatctca aattgcgacc ctaaaacggg gtccttccag
     3061 acttcaattc ttaccggaaa agcatcc
//
LOCUS       DAOQWT010000150         3072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_33910_length_3072_cov_3.089161, whole genome shotgun sequence.
ACCESSION   DAOQWT010000150 DAOQWT010000000
VERSION     DAOQWT010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33910_length_3072_cov_3.089161"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..986
                     /locus_tag="PKJ95_03735"
     CDS             15..986
                     /locus_tag="PKJ95_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNR65390.1"
                     /translation="MVSPLFLGAGIAFILNIIVSRYERIFFPNSQNPFIIKNRRAVCI
                     ILSLLTVLLVLFLFLYILIPQISQFLHLLSIQLPVIYEKSVKWIMSHSEKYPFFEQKI
                     KELDINVETALKKCLEILNNWAFGSVSFIGNVFGKIIEIILAIVFSIYILLFKSTLKN
                     NYQKLSNIYLSSKRKNQLDKIMGIANDTFSNFFLGQFKEAVILGTLCTLGMAILRLPY
                     PTTIGTVVGLSSIIPMVGSYIGATFGVLLIIVVDWVSAVLFLVFIIILQQIESSVIYP
                     KIVGDSIGLPGIWVFAAIIIGSGLMGILGILLGVPIVATIYKLLNQP"
     gene            complement(1086..1271)
                     /locus_tag="PKJ95_03740"
     CDS             complement(1086..1271)
                     /locus_tag="PKJ95_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65391.1"
                     /translation="MRFYLFSKIRFEITEPITLIECYCYQSDFYSKYDVIENSTIGDV
                     NKIGVRLEYLISSGGHI"
     gene            complement(1379..1720)
                     /locus_tag="PKJ95_03745"
     CDS             complement(1379..1720)
                     /locus_tag="PKJ95_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNR65392.1"
                     /translation="MKNIMEYKGYYAKIEYSSEDNTFFGSIIEIDDSITFEGNSVTEL
                     KNAFQEAVDDYLAMCQRIGKEPQKFYKGSFNVRIDPEIHRQAILLAKSKKMSLNKFVE
                     QAISDRIKTSK"
     gene            complement(1722..1976)
                     /locus_tag="PKJ95_03750"
     CDS             complement(1722..1976)
                     /locus_tag="PKJ95_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006508767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNR65393.1"
                     /translation="MTQKEKLIKRLSSIPRDFNINELNKLMQSLGYKEYKKGKSAGSR
                     IAFYHPETKLIVNLHRPHPGNELKVYQIKEIIKFLKKQVI"
     gene            2468..2683
                     /locus_tag="PKJ95_03755"
     CDS             2468..2683
                     /locus_tag="PKJ95_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNR65394.1"
                     /translation="MQQLKIIVEKHPEGYVAYPLGLKGVVIGEGSTYEEALSDVKSAI
                     RFHIDTFGKDIFDNDEILETFIAEVIM"
     gene            2683..2919
                     /locus_tag="PKJ95_03760"
     CDS             2683..2919
                     /locus_tag="PKJ95_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNR65395.1"
                     /translation="MKFPKDAPKQKVIKTLESLGFTIVRIGTHISMIRENPEGTKIPL
                     TMPNHDTIKGSTLRVICRQSGIKRNDFLEKYQKV"
BASE COUNT          983 a          491 c          484 g         1114 t
ORIGIN      
        1 aatacctgtg gggaatagtt tctcctttat ttctcggtgc aggaatagct tttattttaa
       61 atataattgt atctcgttat gagagaattt ttttcccaaa cagccagaat ccgtttatta
      121 ttaagaatcg cagagctgtt tgtattattt tatctctatt aacagtttta ttagtattat
      181 ttttgttcct atacatcctt attccacaaa ttagtcagtt tctccactta ctatcaatac
      241 aattaccggt aatatatgaa aagtcagtaa aatggattat gagtcattct gaaaaatatc
      301 cgttcttcga acagaaaata aaagaattgg acataaacgt tgaaactgct ttaaaaaagt
      361 gtttagaaat tttgaacaat tgggcgtttg gctcagtctc ttttatagga aatgtttttg
      421 gtaaaataat tgaaataata ttggccatag tattcagtat ctatatttta ctttttaaga
      481 gcacactgaa gaacaattat caaaaattaa gcaatattta tttaagcagc aagaggaaaa
      541 atcaattaga taaaattatg ggaatagcta atgatacatt ttctaatttt ttcctgggac
      601 aatttaaaga agctgttata ctaggcaccc tatgcacttt aggtatggca attttgagac
      661 tcccctaccc gactactatt ggcactgttg ttggattatc ctctattatc ccaatggtgg
      721 gatcttatat cggagcgact tttggggtac tcctaattat agttgttgac tgggtaagcg
      781 cggtactttt cttagtattt attattatct tacagcaaat tgaaagcagt gttatttatc
      841 ctaaaatagt tggtgattct atcggcctgc ctggaatatg ggtatttgct gccattatta
      901 taggttcagg attaatgggg attttaggaa tcctgctggg ggttcccatt gtggcaacta
      961 tttataaatt gcttaaccaa ccttaaaatt aactttgagg atgttcaagg attttcttga
     1021 agaggtttat ttcaagaaaa tagaggccca gtttattctt ctattcgctt tcaatctttc
     1081 tgcctttaaa tatgaccacc agatgatatc aaatactcta atcttacacc aatcttattc
     1141 acatctccaa tggtgctatt ctctattaca tcatatttag aataaaaatc actctgatag
     1201 caatagcatt caatcagagt gattggttcg gtaatttcaa atctaatttt agaaaacaga
     1261 taaaatctca taataaggca ctctcttctt ctcgttaaca ataatcgaga attatgtggc
     1321 catggaaagt aaaatgtgtg attacacaaa actgattgct atagacataa acctggtttt
     1381 atttgctggt tttaatacgg tcactaattg cttgctctac aaatttattc aaagacatct
     1441 tcttggattt tgctaataga atagcctgtc tatggatttc agggtctatt ctaacattga
     1501 agctcccttt atagaacttt tgtggttctt tgcctattct ttgacacatt gccagataat
     1561 catctacagc ttcttgaaag gcatttttga gctctgttac actgttgcct tcaaaggtaa
     1621 tactatcatc aatctctata atggaaccaa agaaggtatt gtcttcactg ctatattcga
     1681 ttttggcata ataaccttta tattccatta tgtttttcaa tctatatcac ctgttttttt
     1741 aagaatttaa ttatctcttt tatttgatat acctttaatt cattaccagg atgtggtcta
     1801 tgtaaattaa ctatcagttt cgtctctgga tggtaaaagg caattctgga acctgctgat
     1861 ttaccttttt tgtattcttt ataacctaat gattgcatta gtttatttaa ctcattgatg
     1921 ttaaaatctc ttggtattga acttagccgt ttgatcaact tctctttttg agtcatctaa
     1981 tctctttcat ttaaaattgc aactataatt tagttacatt atattattaa tttggctact
     2041 ttgtcaatcg aaattgtatt taaatggtgt taaaggtccg tccctcaaca ctatttatcc
     2101 accttttttg tcattgcgaa cactgcgaaa gcagtgtgtg gcaatctcat tcatttatac
     2161 ccactgaaaa attatattct tctttgtctt agtgggtcta tattcttatt atctatcatt
     2221 aatcaccccc tgtgataatc gataatttca acatcaaata tatcctatcc attctattgg
     2281 caatacattt tataatgtaa aaacctttgg tcaaatgatg ttagaagctt gaatcctaat
     2341 agattgatat ttttgtataa aacagtctta ggtgttaaaa tatagatact aaattatttt
     2401 attcctactt aaaataaatg aatctgtatt atgttacatt aatcaattga aaaaggagct
     2461 aatttaaatg caacaattaa aaataattgt tgaaaaacac cctgaagggt atgttgccta
     2521 tcctctagga ttaaaaggtg ttgtcattgg tgaaggcagt acttacgaag aggcactttc
     2581 agatgtaaaa tctgctatta gatttcatat agatactttc ggcaaagata tattcgataa
     2641 tgacgagata ttagaaactt ttattgcaga agtaataatg tgatgaagtt ccccaaagat
     2701 gccccaaagc aaaaggtcat aaaaacattg gaaagcctgg gttttaccat tgtcagaatt
     2761 ggtacacata tttctatgat aagagaaaat ccggaaggaa ctaaaattcc attgactatg
     2821 ccaaaccatg atactataaa aggctcaaca ttgagggtta tttgcagaca atctggaata
     2881 aaaagaaatg attttttgga aaaatatcaa aaagtgtagt ttcttattct tggaaactaa
     2941 ggtaatactt caatatttgc ctgtcctatg gaataaactc ttgttcaaat gatgtcaaag
     3001 gtccgtcccc taacatctaa aaggctcaac attgagggtt atttgcagac aatctggaat
     3061 aaaaagaaat ga
//
LOCUS       DAOQWT010000151         3064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_34032_length_3064_cov_3.279495, whole genome shotgun sequence.
ACCESSION   DAOQWT010000151 DAOQWT010000000
VERSION     DAOQWT010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34032_length_3064_cov_3.279495"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            619..2112
                     /gene="lysS"
                     /locus_tag="PKJ95_03765"
     CDS             619..2112
                     /gene="lysS"
                     /locus_tag="PKJ95_03765"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNR65396.1"
                     /translation="MFEQNKEAKSILSIREERIKEIEELRREGIDPFGQSFPGKEAII
                     KVIDKFNQIATGEPSQENVIIAGRIMALRKHGKAAFADLEDQTGKIQVYIKSNFIGTD
                     SFEIFKDISVGDIIGITGTVFKTKTGELTVLANKFTLLTKTLRTLPEKWHGLTDTETR
                     YRKRYLDLIANQEVKELFIERSKIVKYIRNFLDNKGFLEVETPVLQPIPGGATATPFV
                     THHLSLHQDLYLKIAPELYLKRLLVGGLEKVYELNRNFRNEGISTRHNPEFTMLELYE
                     AYADYHTMMEIAEEMVCDILKKLRGTLVIQYQGEMLDFTPPWKRISMYEAIKSVTGIN
                     PDEVKNEEVGDLIVQFQLELPKNAAKGEVINELFEKKVEHTLIQPTFIIDYPVEVSPL
                     SKQKPGYPHLVERFELFINSMEIANAFTELNDPLEQKVRFLQQAESKSEGEINQNFVD
                     YDYIEALEYGMPPAGGMGVGIDRLIMLLTNKDSIREVILFPQLKKKD"
     gene            2116..2382
                     /locus_tag="PKJ95_03770"
     CDS             2116..2382
                     /locus_tag="PKJ95_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNR65397.1"
                     /translation="MRIDKFLKISRVIKRRTEAKYACENNCVRINGRIAKAGDLVKIS
                     DKIEIQFRYKILAIEISRIPDGNIPASSASQLYQVIKEEKIDFD"
     gene            2448..>3064
                     /locus_tag="PKJ95_03775"
     CDS             2448..>3064
                     /locus_tag="PKJ95_03775"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNR65398.1"
                     /translation="MEDKMIIEITEKVKQLLGAHNEEEIKNLVGDIHPNDFSEIAEGL
                     SNEQIIEIFKSIQDQEKVAEYISELNTELQSDLLNAMDKKEASEVLKGMDTDDVVDLL
                     GEIAPEESRELLDLLPKEEAEEIEVLMKYEENTAGSIMNNEFVSLSEYLTVEEAIQHI
                     REMSPEAEMIYYVYVLDKRDKLVGVLSLRDWIVADPHKKISEIMV"
BASE COUNT         1101 a          397 c          677 g          889 t
ORIGIN      
        1 tttagttttt agttaaaatc attgcgggaa taagtgaggg aggttcatag tttatagtta
       61 atagtttttg gtttttggaa taatacaaat ataaataatg tgggtgtaga tggtcgttag
      121 tcgatagtag atggtatttg gtaaaaatgc acaggtaaag actggggtat aaatgaaagt
      181 ggtttatagt taaaatcatt gtgggaataa gtgagggagg ttcatagttt atagttcatg
      241 gtttttggtt tttagtcaaa acatgtggga ataaatgaaa gaataggttt cacagttttt
      301 ggttaatagt ttttagttaa aacacaaaga caaagatgcg ggtaatagtg tatatgattg
      361 ccacgacatg ttttcaacat gtatcgcaat gaccagggcg tttgtgtggg ctatagagta
      421 tgagattgaa taatagtcgt tggtcgttgg tattttgtat ttaggaaaat gtaaagagaa
      481 agaattggtt ataaataaaa ttggtttatg gttaaatgca aagataaaaa tacataatat
      541 ataaaataaa acgtatttaa atacatttat tcaaagagac ctttgaataa atgttagaaa
      601 tagaaggagt gaaattcatt gtttgaacag aacaaagaag ctaaaagcat tttatcaatt
      661 agagaagaaa gaattaaaga gattgaagaa ctaagaagag agggaattga tccctttggt
      721 caaagttttc ctggaaaaga agcaattata aaagttattg ataaatttaa tcaaattgcc
      781 accggtgaac cttcccagga aaatgtaata attgccggaa gaattatggc tctcagaaaa
      841 catggcaaag cagcctttgc agatctggaa gatcagaccg ggaagataca ggtgtatatc
      901 aaatcaaatt ttattggaac tgattccttt gaaatattta aggatatttc ggtaggagat
      961 ataattggaa ttaccgggac agtatttaaa accaaaactg gtgaattaac tgtattggct
     1021 aataaattta ccttactaac caagaccttg cgtactttac cagagaagtg gcatgggtta
     1081 actgacactg aaaccagata tcggaaacgt taccttgatt tgatagcaaa tcaagaggta
     1141 aaggaattgt tcattgaaag aagtaaaatt gttaaatata taagaaattt tctggataat
     1201 aaaggtttcc tggaggtgga gacacctgta ttgcagccta ttcccggagg agccactgca
     1261 accccatttg ttactcatca tttaagttta catcaagacc tttatttaaa aatcgccccg
     1321 gaactctatt taaagagatt gttagtcggg ggtttagaga aagtttatga attgaatcgt
     1381 aattttcgta atgaagggat atcaaccagg cataatcctg aatttaccat gttagaacta
     1441 tatgaagcct atgccgatta tcatactatg atggaaatag ccgaagagat ggtctgtgat
     1501 attttaaaaa agctcagagg aacactggta atccagtacc agggtgaaat gctcgatttt
     1561 actcctccct ggaaaagaat atcaatgtat gaagcaatta agtcagttac cggtatcaac
     1621 ccggatgagg taaaaaatga agaggtagga gatttaattg ttcaattcca gctggagttg
     1681 cctaaaaatg cagcaaaggg tgaagtaatt aatgaactgt ttgaaaaaaa ggttgaacat
     1741 actctgattc agcctacatt tattatagat taccctgtag aagtatctcc tctttctaaa
     1801 cagaaaccag ggtatccgca tctggtggaa cgttttgaac tatttataaa ttctatggaa
     1861 atagccaatg cttttactga attaaacgat cctctggaac agaaagtgcg cttcctgcag
     1921 caggcagaaa gcaagagtga gggagagata aatcaaaatt ttgtggatta tgactatatt
     1981 gaagcattgg aatatggaat gccgcctgcc ggggggatgg gagttggtat cgacaggctg
     2041 ataatgcttc tgaccaataa agattccatc agagaagtaa tactcttccc tcaattaaaa
     2101 aagaaagatt agatattgag aatagataaa tttttaaaga taagccgcgt tataaaaaga
     2161 agaactgaag caaaatatgc ctgtgagaac aactgtgtaa gaataaatgg cagaattgcc
     2221 aaggctgggg atttggtcaa aatttctgat aaaattgaaa ttcaattcag atataaaata
     2281 ttagcaatag agatttcaag aattcctgat ggcaacatcc cggcttcatc tgcttctcaa
     2341 ctgtaccagg taataaaaga agaaaagatt gattttgatt agacggctat aatcttgaat
     2401 aatttaattt gagagtgagt tctgaactta cctcaggggg taaagacatg gaagacaaga
     2461 tgataataga aataacagaa aaagtaaagc aactactggg tgcgcataat gaagaagaaa
     2521 taaaaaactt ggttggtgat atacatccca atgattttag tgagatagca gaaggtcttt
     2581 caaatgaaca gattatagaa atatttaaat ctatccagga tcaggaaaag gtagctgaat
     2641 acatttctga attgaatact gaattgcaat ctgatctgct caatgccatg gataaaaagg
     2701 aagcatcaga agttttaaag ggaatggata ccgatgatgt tgttgacctg ttaggtgaaa
     2761 ttgctcctga ggaatcacgt gaattattag acctgctgcc caaagaagag gcggaagaaa
     2821 ttgaagtatt gatgaagtat gaggagaata ccgcgggcag tattatgaat aatgagtttg
     2881 tttcactttc tgagtatttg actgtagagg aggctataca gcatatccgg gaaatgagcc
     2941 cggaggctga aatgatttat tatgtatatg tgctggacaa aagagataaa ttagtggggg
     3001 ttttatcact cagggattgg attgtggcgg atcctcataa gaaaatttct gaaatcatgg
     3061 taca
//
LOCUS       DAOQWT010000152         3053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_34229_length_3053_cov_3.106404, whole genome shotgun sequence.
ACCESSION   DAOQWT010000152 DAOQWT010000000
VERSION     DAOQWT010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34229_length_3053_cov_3.106404"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1440
                     /locus_tag="PKJ95_03780"
     CDS             211..1440
                     /locus_tag="PKJ95_03780"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR65399.1"
                     /translation="MYYPRHIEPVVQRIAKRKPVIVLTGARQVGKSTMLKKIYNNINY
                     ISLNSPLVRQSAEENPSLFFEVNKPPIIVDEIQKGAKLFDYIKDIIDENGLKGLFYLT
                     GSQSLKLMKNVSESLAGRAGIIRLLGISRRELALLKYREPFQPTVEHMEKLESEQLEL
                     DYEKIIEQIHKGFFPELYEYESNLKDWNDYYSSYFQTYIEKDIRDVLNIRDESAFIKF
                     VKATAALTGNMLNHTTIAEICGKDVKTVKSWLSALESSGLVYLLEPYHNNFNKRLIKS
                     PKLYFLDTGLACWLLGWNTPTQLISGAMWGNIFESFVFSEILKSYYNDGIVKPPLYYY
                     RDKDKNEIDLVIEDGGTLYPVEIKTTSDPKKSMISSFKYLENIPGKDLGTGAIVCLAK
                     QRLPLSDKVWILPVNLI"
     gene            complement(1504..2265)
                     /locus_tag="PKJ95_03785"
     CDS             complement(1504..2265)
                     /locus_tag="PKJ95_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR65400.1"
                     /translation="MPRAARIKSSTGIYHIITRGINQQNIFSSDDDYERFLNTLKRYS
                     TKGGCEIYAYCLMSNHIHLLLKEGVEPLATTMKKIGSSYVYYYNWQYSRKGHLYQDRY
                     KSEPVENDIYFLTVLRYIHLNPIKAGITDSLDSYPYSSYLEYIDKTNIINPIFALNMF
                     HQDKDQAIERFITFHNQPNEDRCLDITEKRETLSDKIIRELVFKRYRIELGALSNTKP
                     ENQKEVLKYLKGLKGCSLRQLSRLTGLTVYQIVKE"
     gene            complement(2378..2650)
                     /locus_tag="PKJ95_03790"
     CDS             complement(2378..2650)
                     /locus_tag="PKJ95_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017896029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65401.1"
                     /translation="MKIAITNRFKRSFQKLTEVEKNTFQKQIELLLRKPHPPFYPSLR
                     IKKIKGTKDLYECSINRTIRMTWQYYQSEYILLRNIGEHDRTLGNP"
     gene            complement(2647..2916)
                     /locus_tag="PKJ95_03795"
     CDS             complement(2647..2916)
                     /locus_tag="PKJ95_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNR65402.1"
                     /translation="MTKIRDNSQITIPKTIMEKLDLKKGDIISFEIRDSEIILKPVAI
                     IDKSQAWFWSKEWQEEEREAEADIKKGRIKSFDNPNELMKELKDK"
BASE COUNT         1002 a          532 c          507 g         1012 t
ORIGIN      
        1 catcgaccaa cgactatcct attcccacat cttttttgca aagaaccgtc ccctgtaaaa
       61 aacatgcttt taaaaaatac agcagaaatt ctaatactcc tttaaaaaga ttgctcttct
      121 tttttagtat atttgacaat tataaattat tcgactataa tggaattaat ttctaatata
      181 gtcgaaaaaa ggaagaggct aatatacatt atgtattatc cccgacatat cgagcctgtt
      241 gtgcaacgta ttgcaaaaag aaagccggta atagtattga ctggtgctcg ccaggtaggc
      301 aaatcaacaa tgctcaaaaa aatatataac aacataaatt atatctcatt gaacagtcct
      361 ctggttcgcc agagtgctga agaaaatccc tcactctttt ttgaggttaa caagccacct
      421 ataatagtgg atgaaattca aaaaggtgcc aaacttttcg attatataaa agatattatt
      481 gacgaaaatg gattaaaagg cctattctac ctgaccggtt ctcaaagttt aaaattaatg
      541 aaaaatgtta gtgagagtct ggccggaaga gcaggaatta taagattgct tggcatctcc
      601 agaagggagc tggctcttct taaatatcgt gagcccttcc agccaacagt agagcacatg
      661 gaaaagctag aatcagaaca actggaactc gattatgaaa aaataattga acagattcac
      721 aaaggctttt tcccggaact ttatgaatat gaaagtaacc taaaagactg gaatgattat
      781 tatagctctt attttcagac atatattgaa aaagacatta gagatgtact caatataagg
      841 gatgaatctg cttttattaa atttgtaaaa gctacggctg cccttactgg aaacatgtta
      901 aaccatacca ccatagcaga aatttgtggt aaagatgtta aaacagtaaa atcatggctt
      961 tctgcattgg aatctagtgg acttgtctat ctgttggagc cttatcataa taattttaat
     1021 aaaagattaa taaaatctcc caaattatat ttccttgata ccgggcttgc ctgctggcta
     1081 cttggttgga acacacctac acagcttata agtggtgcta tgtggggcaa tatatttgag
     1141 agcttcgtct tttctgaaat attaaaaagt tattataatg atggtattgt aaaaccacct
     1201 ttatactatt atcgcgataa agataagaat gaaattgacc tagttattga agatggtggc
     1261 acactctatc ctgttgagat aaagacaacg agtgacccaa aaaaatcaat gataagttct
     1321 tttaaatacc tggaaaatat tcccggtaaa gatctaggaa ctggtgctat agtctgcctg
     1381 gcaaagcaac ggttacctct atccgataag gtatggattt tgcccgtgaa tttaatataa
     1441 aacagtaaaa cataaaacat gggacagttc tctgtttgat gagtttgatg tgtatcatgt
     1501 gtatcattct ttgacaatct gatatacagt caacccggtc aatctggaaa gctgtcttag
     1561 agaacagccc ttcaatcctt ttagatattt caatacctct ttttgatttt ccggtttggt
     1621 attagataaa gctcccaatt caatacgata tcttttaaat accaattctc ttataatttt
     1681 atcagaaaga gtttctcttt tttcagtgat atcaagacat ctgtcttcat tgggctggtt
     1741 atgaaaggtg ataaatcttt caatcgcctg gtccttatcc tggtgaaaca tatttaaggc
     1801 aaatattgga ttgataatat tcgttttatc tatatattct aagtaactgc tataaggata
     1861 agaatcaaga ctatcagtta tacccgcctt tatggggttt aaatgtatat aacgtaaaac
     1921 tgtaagaaaa tagatatcat tctcaaccgg ttcactctta taacggtcct ggtaaagatg
     1981 tccttttctg ctgtattgcc agttatagta atagacataa ctggagccaa tcttcttcat
     2041 ggtagtggca agaggttcca ccccctcttt taacagtaga tgtatgtgat tgctcatcaa
     2101 acaataggca tagatttcac aaccaccctt tgtgctatat cttttaaggg tatttaagaa
     2161 tctttcataa tcatcatcag atgagaaaat attttgctga ttgatacctc tggttataat
     2221 atgataaatt ccagtactgc ttttaattcg agcagctctt ggcataaaga acccctattg
     2281 gaataaaata atcactgaaa actatgaaaa tattctataa taacagagaa aaagtgtcaa
     2341 acaaagaacc gtcccctgta aaacaccctg taaaacatta cgggttgccc aatgttctgt
     2401 catgctcgcc aatattgcgc aatagtatat attctgactg ataatactgc caggtcatac
     2461 gaatggttct attaatactg cactcatata aatctttggt tcccttaatt ttcttgatac
     2521 gtaaagaagg ataaaaaggc ggatggggct ttcttagtag cagttcaatc tgtttttgaa
     2581 aagtattttt ttcaacttcg gttaattttt gaaaacttct tttaaagcga ttagtaatgg
     2641 caattttcat ttatctttaa gttctttcat aagttcattg ggattatcaa aactctttat
     2701 tctgcccttt ttaatgtctg cctctgcctc tctctcctct tcctgccact ctttactcca
     2761 gaaccaggcc tggctcttat cgataatggc aaccggcttt aaaattattt cactgtctct
     2821 gatttcaaaa gaaatgatat ctcctttttt caaatcaagt ttttccataa tggttttagg
     2881 tatggtaatc tgggaattgt ctctgatttt agtcattttg ttcatatatc tcaccttaat
     2941 agcttatgat aaacaatatt atcatatatt ataatattaa tttattaatt tgtaaaatta
     3001 ttaaattagt atatgcgagg aggataacga tggagcaagc tcatttaaat acg
//
LOCUS       DAOQWT010000153         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_34432_length_3041_cov_3.555928, whole genome shotgun sequence.
ACCESSION   DAOQWT010000153 DAOQWT010000000
VERSION     DAOQWT010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34432_length_3041_cov_3.555928"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /gene="pheS"
                     /locus_tag="PKJ95_03800"
     CDS             complement(<1..957)
                     /gene="pheS"
                     /locus_tag="PKJ95_03800"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNR65403.1"
                     /translation="MDKEMENLREKFFKKMASIKNREELQRIRNEYLGRKGIVAQMLK
                     KITSLSPEEKPRIGELLNKIKRDFQKTIAEKDNDFLAENSGKEEQVDISIPGRNFMLG
                     TTHPIELILKYTEEIFMGMGFCIVDGPEIELDYYNFEALNIPKDHPVRDDQDSFYITP
                     DILLRTQTSPMQVRTMESQPPPVRIISAGKCYRRDSIDNTHTPMFHQIEGLAVDKDIS
                     LADLKGVITEFCKQIFGKDRITRFRPGYFPFVEPGVEVDVSCQLCQGLGCQSCGYSGW
                     LEIMGAGMVHPNVLEMAGYNVEKYTGFAFGMGAERIAMLKYGI"
     gene            complement(1319..1675)
                     /gene="rplT"
                     /locus_tag="PKJ95_03805"
     CDS             complement(1319..1675)
                     /gene="rplT"
                     /locus_tag="PKJ95_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNR65404.1"
                     /translation="MPRVTSSVASRNRRKKILKAAKGYRGGKSKLLRTAKDAVRKSLS
                     YAYRDRRAKKRDFRSLWITRIGIAAKIEGFSYSQLMDGLKKANILINRKMLAEMALNH
                     NEGFSELVKIALVESK"
     gene            complement(1738..1935)
                     /gene="rpmI"
                     /locus_tag="PKJ95_03810"
     CDS             complement(1738..1935)
                     /gene="rpmI"
                     /locus_tag="PKJ95_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005211352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNR65405.1"
                     /translation="MPKMKTHRGAAKRFKITESGKIKRAKAYKSHILTKKSAKRKREL
                     AQLTLVDKSDSKRIRRLLPYL"
     gene            complement(1994..2491)
                     /gene="infC"
                     /locus_tag="PKJ95_03815"
     CDS             complement(1994..2491)
                     /gene="infC"
                     /locus_tag="PKJ95_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004696340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNR65406.1"
                     /translation="MNHEIKVPKVRLIGHDGKQLGLVDRDAALEEAQKNNLDLVEVSP
                     DANPPVCRIMDYGKYRYMIEKRVKSGKKKQKATTLKEVKIRPNIGEHDYEFKVRQIHK
                     FLQEGNRVKVTVMFRGREMKFVESGKEILDRVMAETNEFSKVERKPKLEGRNMTLILI
                     SNKSS"
     gene            complement(2641..>3041)
                     /locus_tag="PKJ95_03820"
     CDS             complement(2641..>3041)
                     /locus_tag="PKJ95_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNR65407.1"
                     /translation="PVMLHRTIMGSLERFIGILVEQFAGALPTWLAPVQVKLLPIADR
                     HIEYGQKLEKELFEHNIRVELDENNEKIGAKIRKAEVQKIPYMLVFGDQEEANNSVKI
                     RKRGKGDLGEASLEQFISQIKKEIDEKSFQ"
BASE COUNT          879 a          647 c          404 g         1111 t
ORIGIN      
        1 aatgccatac tttaacatag caatcctttc agcacccata ccaaaggcaa aaccggtgta
       61 tttttcgaca ttataaccag ccatttctaa aacattggga tgaaccatac cagcacccat
      121 aatctccaac cagccactat agccacagga ttgacatccc aggccctgac acagttgaca
      181 ggatacatca acctcaactc ctggctctac aaaggggaaa taccccggtc taaatcttgt
      241 aattctatct ttcccaaaaa tttgtttaca aaattctgtt atgacacctt ttaaatcagc
      301 cagtgaaata tctttatcca cagccagacc ttctatttga tgaaacatgg gggtatgggt
      361 attatctatg gaatcccgcc tataacattt tccagcggat attatccgca cagggggagg
      421 ctgactctcc attgtcctga cctgcattgg agatgtctga gttcttaaaa gaatatccgg
      481 ggtaatatag aaagaatcct gatcatctct taccgggtgg tctttgggaa tattaagtgc
      541 ttcaaaatta taataatcaa gttcaatttc cggcccatcg acaatacaaa accccattcc
      601 cataaatatt tcttcagtat atttcaagat taattcaata ggatgagtgg tccccagcat
      661 aaagtttcta cctggaatag agatgtctac ctgctcttct tttccagaat tttctgctaa
      721 aaagtcatta tccttttcgg ctattgtttt ttgaaaatct ctttttattt tattaagtaa
      781 ttcaccaatt ctaggcttct cttccggcga caacgaagtt atcttcttca acatttgggc
      841 tacaattcct ttacgtccca aatattcatt tcttattctt tgtaattctt ccctgttttt
      901 gatagaagcc atctttttaa agaatttctc tcttaaattt tccatctctt tatccaaaag
      961 aatccctccc tatctcatca tattataaaa aaagaagtct ataattttct ccttaaataa
     1021 agggacgaaa attataaact tctccgcgtt accacccttt ttgacttatt attaaagtcc
     1081 aaacttattt tatctctaac gggataatcc gtcctggcct atttaataat tttcagccgg
     1141 cttattaaaa gtgaaattca ataaaacctg ctattctttt cttgaatata atgcgagttt
     1201 tatctactat cttttagtat ttctcagttt ttttatactt tttatttata tattttttat
     1261 aaaaaactaa aaatttcttc aaaaaatttg gggttataag tatcgcaaat aaatatattt
     1321 atttgctctc taccaaagca attttaacca actcggaaaa accctcatta tggtttaacg
     1381 ccatttcggc taacattttt ctatttatta aaatattagc ttttttgagt ccatccatta
     1441 attggctata ggaaaaccct tcaattttgg cagcaatacc aatacgggtt atccaaagac
     1501 tacgaaaatc tcttttttta gctctcctat cacgataggc gtaagacaaa gattttctca
     1561 ctgcatcttt tgcagtacgt aatagcttac tcttgccacc acgataccct ttggcagctt
     1621 ttagtatctt ttttcttcta tttctcgaag caacgctact ggtaactctt ggcatgacta
     1681 actctcctca ttcagctcat cattcatttt aaaaatactt tttaataaag acagattcta
     1741 taaatagggt aataaacgtc ttattctttt actatcactt ttatcaacta aagtcaactg
     1801 ggctaattct ctttttcttt ttgcagattt tttagttaat atatgacttt tataagcttt
     1861 agcacgttta attttcccgg attcagttat tttaaaacgt tttgcagcac cacgatgtgt
     1921 tttcatctta ggcataatta atattcctcc ataaataaag tcaaaggcat tataaccctg
     1981 attaacattc tgttcaacta cttttattag aaataagaat tagggtcata tttctccctt
     2041 ctaatttcgg ttttctctct accttagaga attcatttgt ttcagccata acacggtcta
     2101 aaatctcttt acccgattca acaaatttca tttctctccc cctgaacatt acggtaactt
     2161 taacccgatt accttcctgt aaaaatttat gaatttgtct taccttaaat tcataatcat
     2221 gttctccaat gtttgggcga atcttgacct ctttgagagt agtagctttt tgtttctttt
     2281 tacccgattt tacccttttt tcaatcatgt agcgatattt accataatcc ataatacgac
     2341 aaaccggggg gttagcatca ggggaaacct ccactaaatc aagattgttt ttttgtgctt
     2401 cttctaaggc agcatcccta tcaaccagtc ccagttgttt tccatcatga ccaattaagc
     2461 ggactttggg aaccttaatt tcatgattca cccgaaaagt agattttgac tttatctcac
     2521 attcctcctt ttgcactcat tactgttaat tttatattct taattattag gaatcttgtt
     2581 aaatgaatat agcatatagt ttttttcaag tcaatatagt gttgtaagac gatttaatct
     2641 ttattgaaaa gatttttcat ctatttcttt cttaatttga ctgataaact gttccagtga
     2701 ggcttctcct aaatcacctt tacccctttt tcttatcttt accgaattat tagcttcttc
     2761 ctggtctcca aaaactaaca tataggggat tttctgaact tctgctttcc tgatttttgc
     2821 cccaattttt tcattatttt catcaagctc tacccggata ttgtgttcaa atagctcttt
     2881 ctccaatttc tggccatatt caatatgtct atcggcaatg ggaagcaatt ttacctgaac
     2941 tggagcaagc caggtgggca aagctccggc aaactgttct accaatatac ctataaagcg
     3001 ttccaggctt cccataatag ttctatgcaa cataaccggt c
//
LOCUS       DAOQWT010000154         3033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_34554_length_3033_cov_2.903627, whole genome shotgun sequence.
ACCESSION   DAOQWT010000154 DAOQWT010000000
VERSION     DAOQWT010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34554_length_3033_cov_2.903627"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PKJ95_03825"
     CDS             complement(<1..600)
                     /locus_tag="PKJ95_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR65408.1"
                     /translation="MLKLINIHKCFAKGTSHETNALRGIKININEGDFLTVIGSNGAG
                     KSTLLNIIAGTILPDKGRVRINGQDVTNKEEHFRARFIGRVFQDPLMGTAPSMTIEEN
                     LALSIKRFGRGLNLGLNKLNREYFRNKLAQLELGLEDMITKKVSLLSGGQRQALTVLM
                     ATMFDPKILLLDEHTASLDPEISKKIQSITQDIVQEKRLT"
     gene            complement(619..1548)
                     /locus_tag="PKJ95_03830"
     CDS             complement(619..1548)
                     /locus_tag="PKJ95_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR65409.1"
                     /translation="MTWSLLVHALEQGLVFSIMALGVFITFQILNFPDLTVDGSFPLG
                     AAISAKIIFAGGDPFIAVIWAMFGGVLAGGLTAFFHTRLKFTNLLSGILTMTLLYSVN
                     LRIMGKSNIPLLGKVTILEVIQSWFPQLSSDQLTPVLFLFVVFSVKFLLDYFLGTEIG
                     MAIRATGDNEQMIRSLGVDTSGTKIIGLCLSNGLVALSGSLFAQYSGFADVGMGIGTI
                     VSGLASVIIGRSLIRNRGSINWMTVSVIIGSIIYRTTVMVALRYGYRLGFQPGDLKLI
                     SVLLVVISLTFPSFRGKIKTIILNNGSNGVENE"
     gene            complement(1585..2610)
                     /locus_tag="PKJ95_03835"
     CDS             complement(1585..2610)
                     /locus_tag="PKJ95_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR65410.1"
                     /translation="MKKNKKFRSIRIFLCITLIFVLLLSLSNSISAEQLKIGISQIVR
                     HPALDACREGFISGMEKAGYAEGTDVVYDFQDAQGEFSNAIAIMQKFKDDKVDMIVAI
                     STPIVQAAAQVTSEIPVIIGAITDPVAANVVKSWESSGNNLTGMSDAAPNRTQLELIP
                     RFFPKAKRVGTIYNAGEANSVVQVELSQEICQELGLELVEVTASNSAEVLMAVQSLVG
                     RVDLFYIVTDNVAVSAFSSIVKVSLEEKIPIIVADPTMVLEGALASFGIDYFSLGEKS
                     AEKAVQVLQGKLPTEVPIGKLENPEDLTFIVNIDNARQLNLDIPKELIDEADSIVFSG
                     FVWDRSE"
     gene            2688..2816
                     /locus_tag="PKJ95_03840"
     CDS             2688..2816
                     /locus_tag="PKJ95_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65411.1"
                     /translation="MTNTSPVPDLLIKKPRMFLHPWLKEKPPALPEVNAGGSIGFA"
BASE COUNT          949 a          659 c          495 g          930 t
ORIGIN      
        1 agtcaatctc ttttcctgca caatatcctg ggtaattgac tgtatttttt tgctgatctc
       61 cgggtccagt gaggcggtat gctcatctaa caataatatc ttggggtcaa acatggtcgc
      121 cattaaaaca gttaaagcct gcctctgacc tccggataac aaagatactt ttttggttat
      181 catatcctcg agacccaatt ccagctgggc taatttattt cgaaaatatt ctctgttcag
      241 tttatttaat cctaaattaa gccccctgcc aaaacgtttg atggaaaggg ctaaattttc
      301 ttctatagtc atagagggtg cagtccccat tagagggtct tgaaataccc ggcctataaa
      361 tcttgcccga aaatgctctt ctttattggt tacatcctgg ccattaatcc ttactctacc
      421 cttatcagga agaatagttc cggcaataat attcaagagt gtggatttac cagcaccgtt
      481 actgcctata acagttaaaa aatctccttc attgatattt attttgatac ctcttaaggc
      541 attggtttca tgagaggtac ctttggcaaa acatttatga atatttatca gttttaacat
      601 aacaggacaa cctccttttt actcattctc tactccatta gaaccattat ttagaataat
      661 agttttaatt ttccctctaa aagagggaaa ggttagtgat ataactacta acagtacaga
      721 gattaatttt aaatctcctg gttgaaaacc aaggcgatat ccatagcgta aggctaccat
      781 aacagtagtc cggtatataa tagaaccgat aatgacactg acagtcatcc aattgatgct
      841 gcctctattt ctgattaaac tccgtccaat gataactgag gctaatccgg aaacaatggt
      901 tccgattccc attcccacat cagcaaaacc ggaatactgt gcaaaaagcg aaccggaaag
      961 agccactaat ccgttagaga gacaaagacc tataatcttg gtaccactgg tatcaacacc
     1021 caggctgcga atcatctgct cattatcacc ggttgctctt atagccatgc caatttcagt
     1081 acctaaaaaa taatcaagta aaaatttaac tgaaaatacc acaaaaagaa agaggacagg
     1141 agtcagctga tctgaagaaa gctgtggaaa ccagctttgg ataacttcaa gaattgtaac
     1201 ttttcctaaa aggggaatat tggattttcc cataattctc aagttgaccg agtagagtaa
     1261 ggtcatagtc aatatcccgg aaagtaaatt agtaaacttt aatctggtat ggaaaaaagc
     1321 agttaatcca cctgccagta cacctccgaa catagcccaa atcaccgcta taaaggggtc
     1381 ccctccagca aagataattt tagcgctgat agctgcccct aaggggaaag aaccatcaac
     1441 cgttaaatca ggaaaattta atatctgaaa ggtgataaaa actcctaatg ccataatact
     1501 gaaaacaagg ccttgttcta aggcatgtac taacaagctc caggtcatat tatataaact
     1561 ccttcctctt tctaattctt tgtattattc actgcggtcc cagacgaaac cggaaaagac
     1621 aattgaatct gcctcatcta ttaattcttt gggaatgtca aggtttaatt gcctggcatt
     1681 atcaatattt acaataaaag tcaaatcttc aggattttct aacttaccaa tgggcacttc
     1741 agtgggtaat ttaccctgta agacttgaac tgccttctca gcactttttt cacctaaact
     1801 gaaatagtct attccaaaag aagctaaagc cccttcgaga accatagtcg ggtcagctac
     1861 gataatagga attttttctt ccagtgaaac ttttacgata gaactgaagg cagaaactgc
     1921 cacattatca gttacaatat agaaaagatc tacccgtcct accagagatt gcactgccat
     1981 caggacttcg gcagaattac tggcagttac ctctaccaat tccagaccca gttcctgaca
     2041 tatttcctga gatagctcta cctgtaccac tgaatttgct tcaccagcat tataaatagt
     2101 tcccacccgt ttagcttttg ggaaaaacct gggaattagc tccagttgtg tcctgttggg
     2161 agcagcatca gacattccgg taagattgtt tcctgaactt tcccagcttt taacaacatt
     2221 agcagctaca gggtccgtaa ttgctccaat aattacaggt atttcgctgg taacctgtgc
     2281 agcagcctga acaatggggg tggaaattgc cacaatcata tcaaccttat catccttaaa
     2341 tttttgcatg attgctatgg cattgctaaa ttccccctgg gcatcctgaa aatcatagac
     2401 aacatcagta ccttcagcat agccggcttt ctccatcccg gagataaaac cttcccggca
     2461 ggcgtccaga gccgggtgtc taacaatctg actgatacct atctttaact gttcagcgga
     2521 aatagaattg ctcagtgaca acaaaagaac aaaaatcagg gttatacaca gaaatattct
     2581 aattgaccta aacttcttat ttttcttcat ttttcttctc cttctttaca aaattattct
     2641 aatctcatat tactttatac tcttatcttg ttcttgccct tctctttatg actaacacct
     2701 ccccggtccc tgatttatta ataaaaaaac cacgaatgtt tttacacccg tggttaaaag
     2761 aaaaaccacc ggctttacca gaggtaaatg ccggtggctc tattggcttt gcctgagtta
     2821 attaaaatta actactacca aacccgatag gcatctacct ccggattata atagtagtaa
     2881 taataagcat tattcaaatt ggaattatgg ttaatattca ttttattttt tgcaatccca
     2941 aattttttaa tcattttact tcttattttt taaaaagtca aattatttat aatatttttg
     3001 agtattttgt aacttttata ctaaattttc att
//
LOCUS       DAOQWT010000155         3030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_34587_length_3030_cov_3.462185, whole genome shotgun sequence.
ACCESSION   DAOQWT010000155 DAOQWT010000000
VERSION     DAOQWT010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34587_length_3030_cov_3.462185"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKJ95_03845"
     CDS             <1..370
                     /locus_tag="PKJ95_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65412.1"
                     /translation="PDIPDTLGLVTEKNIKIPKKDIFNNIVPNNIEINAIMMALGTSF
                     YFQGFKDDIKDTLTVYGSFIQKQRGPVGTFSSNKRTGYNKDYHFDTRMNVNNPKFGEV
                     LPPYFPTTGKYVKLWWKEVD"
     gene            476..742
                     /locus_tag="PKJ95_03850"
     CDS             476..742
                     /locus_tag="PKJ95_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65413.1"
                     /translation="MSSLIRIRNIIYIILTLIIIIAISYAMAYIVRQNKEYARLNQES
                     LVREKVEEIVKQESNSEKNLLEIKEDRVERGDTDFRVGNDVIIF"
     gene            1085..2299
                     /gene="pilM"
                     /locus_tag="PKJ95_03855"
     CDS             1085..2299
                     /gene="pilM"
                     /locus_tag="PKJ95_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNR65414.1"
                     /translation="MLAIDFGARSIKAVKLNRTGSGYYLDNFGIVLTPPGTILHGEIK
                     DVEAVSQALNKMLHKKRLYDKGNIIAITGQKVIVREIITPLMAEKELSEGIRWEAPKY
                     VPYDLKDSLLDVKKIEELEEKEGTRMMKVLLVATPRSVVDQQIAVLKNVRIQPRVVDI
                     VANAQIRAFESYLTPTSVDEDGTMIDIILSMGASTTEITLMEGDQLKFTRTILKGGGD
                     VTELIVKKLGIPFQEAERLKRHIGLSLPEEKTVPDSDLKDTKDLKEEQDEKDEKEDQE
                     LVEIPSQQAGEEQVSPQIKDEDTSTPTEEEIAGLVKTGVNEIFNEIRKSLNYFKTQYQ
                     KVNYQRIILCGGMAQLPQLGQMLKKQFGITVVIANPLKNITINEHNIDIESLDKYKSV
                     LATAIGLAKRER"
     gene            2310..2903
                     /locus_tag="PKJ95_03860"
     CDS             2310..2903
                     /locus_tag="PKJ95_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65415.1"
                     /translation="MAKTKIVSRDINLLPLEYREKEKFNILRILITTFIIVLIAASGY
                     LYFFLESEILTRENEVKNLQTQLAIVEKGIEEVKNLEKDREELARRVNVIENLIKNQS
                     SLTHVLGDFSTTALPEVWLNDLSVSANQTFNFTANTFNNYLIARYMNTLKDFERFDAI
                     ELQYIHKESTEIPEQNKKIDTVKFQLSGIFIPFSKNF"
BASE COUNT         1147 a          447 c          545 g          891 t
ORIGIN      
        1 tcctgatatt cccgatacat taggactggt tactgaaaaa aacatcaaaa ttccaaaaaa
       61 ggatattttc aataacatag ttccaaacaa tattgagata aatgccatta tgatggcatt
      121 gggtacatct ttttattttc agggatttaa agatgatatc aaggatactc tgacggtcta
      181 tggctctttt attcagaaac aacgtggccc agtaggtacc tttagcagca ataaaaggac
      241 gggttataat aaagattatc attttgacac caggatgaat gtaaataacc ccaagtttgg
      301 agaagtgctt cctccctatt tcccgactac cggtaaatat gtcaaactat ggtggaaaga
      361 agtagattaa aaatatttta gaatattttt attttgtact attatttgag taattaatag
      421 taaaaaaaga ttagtatcct tgtattcata ctcaggaaac aggaaaagat attagatgag
      481 ttctttaata agaatcagaa atataattta tattatacta acattgataa ttataatagc
      541 aataagttat gcaatggcat atattgtcag gcagaataaa gaatatgcca gattaaatca
      601 ggaatcatta gttagagaaa aagttgagga gattgttaaa caagaatcta attctgaaaa
      661 aaatttactt gaaataaagg aagatagagt agaaagaggg gataccgatt tcagagtagg
      721 taacgatgta ataatatttt agatgaaaat gaaatagttt aaccagcatc tcttaatact
      781 actaataatt aataatatca actatagctt tttaaaaaat ttttttgaaa attcatacaa
      841 attagtgaat caataaatat tttatatatt tatatataac aatttaaaga aatattttaa
      901 aaatagcttg tttaattata ttgctatata ttataataaa aacaataagc gtttttgatt
      961 gtttaatagc ttaaaataaa aatagttgtt ctaaattatt atttcctgct ataatatgaa
     1021 aagattaaag acttgccccc taagcagaac ccagaggcag gtttataatg gcaaaggagc
     1081 atagatgctg gcaatcgatt ttggcgctcg ttctattaaa gcagtaaaac tgaacagaac
     1141 tggaagcggg tattatttag ataactttgg aattgtatta acgccgcctg gtactatttt
     1201 acatggtgag ataaaggatg ttgaggcagt aagccaagct ttaaataaaa tgcttcataa
     1261 aaagagactt tatgataaag gtaatattat tgctattacc ggtcaaaaag ttattgtccg
     1321 cgaaatcatt acacccctga tggctgaaaa agaactttca gaaggaatta gatgggaagc
     1381 tcccaaatat gtcccctacg atttaaagga ttccttactg gatgtcaaga aaattgagga
     1441 gctggaggaa aaagaaggga ccaggatgat gaaagtgctg ctggtagcca ctccgagaag
     1501 tgttgttgac cagcagattg cagtgctaaa aaatgtcagg attcaaccca gggtcgttga
     1561 tattgtggca aatgcccaga tcagggcttt tgaaagctac ctaaccccca cctctgttga
     1621 tgaagatggc actatgattg atatcattct ttctatggga gcaagtacca ctgagataac
     1681 gttaatggaa ggggaccagt taaaatttac ccgaactatc cttaaaggcg gaggagatgt
     1741 taccgagcta attgtgaaga agcttggtat accatttcaa gaagctgaac ggctcaaaag
     1801 gcatatcgga ctatccttac cggaggaaaa aaccgtcccg gatagcgatt taaaagatac
     1861 aaaagattta aaagaagaac aagatgaaaa agatgaaaaa gaagaccagg aattagtaga
     1921 aataccctcg caacaggcag gagaagaaca ggttagtcca caaataaaag atgaagatac
     1981 ctctacccca accgaagaag agatagccgg tttagttaaa actggtgtca atgaaatttt
     2041 taacgagatt cgtaaatcat taaattattt taaaacacag tatcaaaagg taaattatca
     2101 gagaattatt ttatgtggtg gaatggcaca attgcctcaa ctcggacaaa tgttgaagaa
     2161 acaattcggc atcactgtag taattgctaa tcccttaaag aacattacta taaatgagca
     2221 taatattgat attgaaagct tggacaagta taagtctgtc cttgctactg ctattggatt
     2281 agccaaaaga gaaaggtaaa tgatatatta tggctaaaac taaaattgtc tccagagata
     2341 tcaacctctt gccgctggaa taccgggaaa aagaaaaatt taatattctg agaatattaa
     2401 taacaacctt tataattgtt cttattgctg cttctgggta tttgtatttc tttctagaaa
     2461 gtgaaatact taccagagag aatgaagtaa aaaacctgca aacccaacta gcaattgttg
     2521 aaaaaggcat agaagaagtt aaaaatttag aaaaagaccg agaagaatta gccagacggg
     2581 taaatgttat tgaaaatcta attaaaaatc aatcatcact aacccacgtt ttaggtgatt
     2641 tcagcacaac tgctttaccc gaagtatggc ttaatgacct cagtgtcagt gccaaccaga
     2701 cctttaattt taccgccaat acctttaata attatcttat tgcccgctat atgaacactc
     2761 taaaagattt tgaacgcttt gatgctattg aattgcagta cattcacaaa gagagtactg
     2821 aaattcccga acaaaataaa aaaatagata cagtaaaatt tcagttatcc ggtatcttta
     2881 tacctttttc aaaaaatttc taaccataag aacgaaggca aataaataga ggtgaataat
     2941 tttgaagttt tttaacctga gcaaaagagc aaaaatagtg atatttgtaa ttttcatttt
     3001 agtttttgct taccttaatt attcctatat
//
LOCUS       DAOQWT010000156         3028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_34628_length_3028_cov_3.167844, whole genome shotgun sequence.
ACCESSION   DAOQWT010000156 DAOQWT010000000
VERSION     DAOQWT010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34628_length_3028_cov_3.167844"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PKJ95_03865"
     CDS             complement(<1..402)
                     /locus_tag="PKJ95_03865"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNR65416.1"
                     /translation="MLTNKLAPSILNADFSCLREVLFLLEKCKVDLIHLDIMDGNYVP
                     NITFGPKIVSTIRENTNLPLSVHLMIEKPERYINEFAKILHVQDSIIVHVETCSHLDK
                     TLQMIIDTGLKSGVALNPSTSLEMVRYVLDKL"
     gene            complement(437..1972)
                     /locus_tag="PKJ95_03870"
     CDS             complement(437..1972)
                     /locus_tag="PKJ95_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNR65417.1"
                     /translation="MISKLCSAALLGVEAFLVEVEVDVANGLPSFNIVGLPDTAVQES
                     RERVRAAVKNSEYQFPMERITVNLAPADIKKEGPALDLPIAVGILMANKHLHRLSSIN
                     YIIIGELSLNGSIRPVNGVLSIALMAKKNGISSIIVPKENAEEAGIIDGINIYPVSNL
                     RQVVRFLNKEINIEPFYVDKEKLMSNINIYSIDFADVKGQQLAKRALEVAAAGGHNVI
                     MIGPPGSGKTMLAKRLPTILPSITLQESIEITRLYSISNFTNQNFPIMTSRPFRAPHH
                     TISDIGLIGGGTFPKPGEISLAHNGVLFLDELPEFSRSALESLRQPIENGKVTISRAL
                     RSVTYLSRFMLVAAANPCPCGYFGDSVKQCSCSSYQIQKYRARISGPLLDRIDIHIEV
                     PRLDYKELVKRNNDNESSSEIRKRIEKSRQIQLNRFKGMNIYNNSQMSRKDIEKYCSL
                     SKEAWNLLNQAMEKLKLSARAYDKILKLSRTIADLDLREEVQTAHIAEAIQYRSLDRE
                     YLI"
     gene            complement(2014..2367)
                     /locus_tag="PKJ95_03875"
     CDS             complement(2014..2367)
                     /locus_tag="PKJ95_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNR65418.1"
                     /translation="MNNTGSIGEKLAKYYLSQKGYHILEQNYRAIFGEIDIIAEKNNC
                     ICFVEVKCRTSDKYGFPEESITAHKKNKIIKVAQLYIKQNRIEDKLFRFDIIALNFNN
                     FSLTRIKHITNAFYQ"
     gene            complement(2392..>3028)
                     /locus_tag="PKJ95_03880"
     CDS             complement(2392..>3028)
                     /locus_tag="PKJ95_03880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNR65419.1"
                     /translation="QEKIRIEKLKSVEQGLSLSGFHYIAGIDEVGRGALAGPVVAAAV
                     VIKNINSFFITGLKDSKKINKTKREYLSKLIIENSCDIGVGLVNPATIDRINILKATL
                     LAMKRAIDSLNQRPDYLLIDALRIPYIGIEQNNMINGEDNSISIAAASVVAKVFRDNL
                     MEKFHNKYPLYLFNKNMGYGTKKHLAAIKAYGICPIHRKTFKGVLIDKIDL"
BASE COUNT          910 a          562 c          476 g         1080 t
ORIGIN      
        1 taatttatct aagacatatc ttaccatttc cagcgatgta gaaggattaa gagccactcc
       61 tgattttagt ccagtatcaa ttatcatctg taatgttttg tctaaatgag agcatgtttc
      121 aacatgaaca ataatactgt cttgaacatg caatattttg gcaaattcgt taatatatct
      181 ttctggtttt tctatcatta gatgtacact taagggaaga ttagtatttt ctctaatggt
      241 gctcactatt ttagggccaa aagttatatt gggaacatag tttccatcca taatatccag
      301 atgtattaaa tctactttac atttctctag aagaaatagc acttctctca ggcaggaaaa
      361 atcggcattt aatatcgatg gagccagttt atttgtaagc attttatatt tacctcttta
      421 cctttaaacg aatattttat atcagatatt ctctatctaa actcctgtat tgtattgcct
      481 cggcaatgtg tgcagtctga acttcttccc ttaaatcaag atcggcaatg gtcctggata
      541 gttttaaaat tttatcatat gctcgtgcac ttaattttaa tttttccatt gcctgattaa
      601 gcaaattcca ggcttcttta gataatgagc agtatttttc tatatctttc cggctcatct
      661 gtgaattatt ataaatattc atgcctttaa aacgattcaa ctgtatctgc ctcgattttt
      721 cgattctttt tctaatttca ctggaacttt cattatcatt atttcttttt actaattctt
      781 tataatctag ccttggaact tcaatatgaa tatctattct atccaacaga ggacctgaaa
      841 tccttgccct atatttttga atttgatagg aactacagga acactgtttt acggaatcac
      901 caaaataacc acaaggacag ggatttgcag ctgccacgag cataaagcgc gataaataag
      961 taactgaacg taatgctctg gaaatggtaa ctttcccatt ttctattggt tgacgtaacg
     1021 attctaatgc tgaacggcta aattcaggaa gttcatccag gaacaagacc ccattgtgag
     1081 ccaggctgat ttctccgggc tttggaaagg ttcctcctcc aattagccct atatcactaa
     1141 tagtatgatg tggtgctcga aagggcctgc tggtcataat aggaaaattt tgattggtaa
     1201 aattagaaat actatagaga cgggttattt caatagattc ctgtaaggtg atactgggta
     1261 gaattgtggg aagacgtttt gccagcatag tcttaccaga acctggtggg ccaatcatta
     1321 ttacattatg cccacctgct gcggcaactt ccagggctct tttagctaac tgttgccctt
     1381 taacatcggc aaagtcaatg gaataaatgt taatattaga cattaatttt tctttatcca
     1441 cataaaaagg ttcaatattt atttctttat ttagaaatct aactacttgt cgtaaattag
     1501 aaacaggata gatgttaatt ccatctatta ttccagcttc ttcagcattt tccttaggaa
     1561 caattatgct actaataccg ttttttttgg ccattaaggc gattgataac actccattta
     1621 ccggtcttat acttccgtta agagacagtt cccctataat aatataattt attgaggaaa
     1681 gcctgtgtaa atgcttgttg gccattaata tacccacggc tattggcaaa tctaaagcag
     1741 gtccctcttt tttgatatca gcaggtgcca gatttacagt aatcctttcc ataggaaatt
     1801 ggtattcgga attctttaca gcagccctaa ctctttctcg tgattcctgt actgcagtat
     1861 caggtaaacc gacaatatta aaagacggca acccattggc aacatccact tctacttcaa
     1921 caagaaatgc ttctacacct aaaagggcag cactgcaaag ttttgagatc aagggaacac
     1981 ctatactgtt aattatattt attactacgt tgtctattga taaaaggcat tagtaatatg
     2041 ttttatccgg gttaatgaaa aattattaaa atttaaggca ataatgtcaa aacgaaatag
     2101 tttatcttca atcctatttt gtttaatgta caattgagca acctttataa tcttattttt
     2161 cttatgtgca gtaattgatt cttcggggaa tccatattta tctgatgttc tacattttac
     2221 ttcgacaaag caaatgcaat tatttttttc tgcaatgata tcaatttctc caaagattgc
     2281 tcgatagttt tgttctaaaa tatgataccc tttctgactg aggtagtatt ttgctaattt
     2341 ttcaccaatg gaacctgtat tattcatatg gtattatttt tttctatagt cctacaaatc
     2401 tattttatct attaatacac ctttaaatgt tttgcgatgt attggacaaa ttccatatgc
     2461 cttaattgca gctaaatgct tttttgttcc ataccccatg tttttattga acaaatagag
     2521 cggatactta ttatgaaatt tctccataag gttatcgcga aatactttgg caaccaccga
     2581 tgcagctgct atagaaatac tgttatcttc accattaatc atattattct gttctattcc
     2641 aatatagggg atcctaaggg catcaattaa cagataatca gggcgctgat taagagaatc
     2701 aatggccctt ttcatggcca gcaatgttgc ttttaatatg tttattcggt cgatagtagc
     2761 aggattaact aaaccaaccc ctatatcgca agagttttct ataattaatt tagataagta
     2821 ttcccgcttg gttttattaa tttttttaga atcctttaaa ccggttataa agaaagaatt
     2881 aatattttta atgactacag ctgctgcaac aactggccca gccaaagccc ctcttcctac
     2941 ttcatcaatt cctgcgatat agtgaaaacc ggaaagggaa agtccttgtt caacactttt
     3001 taatttttca attcttatct tttcctga
//
LOCUS       DAOQWT010000157         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_34697_length_3024_cov_2.751431, whole genome shotgun sequence.
ACCESSION   DAOQWT010000157 DAOQWT010000000
VERSION     DAOQWT010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34697_length_3024_cov_2.751431"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PKJ95_03885"
     CDS             complement(<1..1123)
                     /locus_tag="PKJ95_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65420.1"
                     /translation="MEDTLLKEVHFPLEVVILLIAGLALLITGILLFPVSMGILPYYE
                     NGLYGLFLVLFALQTIGLGKTPFGDAPRSNVVVAFGIFIAGIGVITCFIPGILGPIPA
                     ILLFICFSLGGLSLLLQLFLDKEKYKNWIKYGGIFNHLIAGCGSVYILSILIGLLVIK
                     PSLITTKMVAVLVFIYGIAVIYLASILKSIYHKYPEAETSSKGSAGLSTDKVLILLLS
                     VLMLILGLLLIPVCLGRIPFAPNAQLGLLMTIFAIQMMALGNTPVGPFPRNWLMFLLG
                     LLFASLGIVSIIIPGILVGRLTILVGLLNISGGIVSLWKTLTPHFQKREKTQEPSHPI
                     LTKLFITQLTLGLLSVLFGTSMLVSNLLPGIIVGIILTAN"
     gene            complement(1156..1962)
                     /locus_tag="PKJ95_03890"
     CDS             complement(1156..1962)
                     /locus_tag="PKJ95_03890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR65421.1"
                     /translation="MTDYFKDKVAVVTGAASGIGLGLSEHLLKRGAKAVFMGDFNKEN
                     LDKEASRLNAEFPGKVHPLLTDVTKLEQVEELIKQAKMLGGHLDFVFNNAGMGMTLPT
                     EQITFDIWKMLIDLNLWGVIYGTYSAIPIMREQGFGHIINTASIAGCVPIPYQAVYAT
                     TKSAVISMTESLQYELEVEGLQFSVFCPGNVITSIFGSMTPPPDAITVDEAVEYIFQE
                     LEKKSMIIIFPQNMREAESLYRENRAEFDKMIRGLAAERRENYRTKGTYY"
     gene            complement(2013..2810)
                     /locus_tag="PKJ95_03895"
     CDS             complement(2013..2810)
                     /locus_tag="PKJ95_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HNR65422.1"
                     /translation="MKGKFKIREDFVYKMPVHFGGSEFYPVRTVYGDNTGIVIQYETA
                     QEALLQYIPEVFELIEPVITLQYNNCRDVDWMAGGEYRLIQLTAPVKYTGNSEGLTGD
                     YALVVWENLTCPIVGGREEDGVPKIFADISSERHYGDYWFTSASYESNTFLKLGFTRK
                     EELAQSAIDRKNEDPKINLFGWRYLPNLGKGGTSLSHATLYPQEVVVKKMWAGEGTLE
                     WNILKPEEHPLQAHIIRALAELPIVKYTGGNMIKATARLNVGDSRAL"
BASE COUNT          913 a          681 c          551 g          879 t
ORIGIN      
        1 cattggcagt caagataatt ccaactatta ttccgggaag gaggttagag accagcatag
       61 atgttccaaa aaggactgac agtaaaccca gagtcaactg ggttataaac agttttgtta
      121 aaatgggatg ggatggctcc tgggttttct cccttttttg aaagtgagga gtaagtgttt
      181 tccacaggga aacgattcca ccactaatat ttaataaccc caccagtata gtcaatcggc
      241 caactaaaat cccgggaata atgatagata cgattcctaa agaggcaaat aaaagcccta
      301 gtaagaacat cagccagtta cgggggaatg ggccgactgg tgtattgccc agtgccatca
      361 tctgtatggc aaaaatcgtc ataagcagac ccagctgggc attaggcgca aagggaatac
      421 gccctaaaca tactgggatg agcaataacc ccagaatgag cattaaaaca ctgagcagta
      481 aaatcaacac tttatcagta gaaagtcccg cactcccctt ggaactggtc tcagcttccg
      541 gatacttatg ataaatactt tttagaatag aggcaaggta tataacggct attccgtata
      601 taaaaaccaa taccgccacc atttttgtgg taatcagcga gggctttatt accagaagac
      661 caatcagtat tgataatata tatacagacc cgcatcccgc gattaagtga ttaaaaatcc
      721 ctccatattt aatccagttc ttatattttt ctttatcaag gaacaattgc agaagcagtg
      781 aaaggcctcc caagctaaaa cagataaata ataagatggc aggaatcggc cctaagatac
      841 ccggaataaa gcaggtaata acaccgatac cggcaatgaa tatcccgaat gctactacca
      901 cattggaacg gggagcatca ccaaagggtg ttttccccaa tccgatggtc tgtaatgcaa
      961 ataagaccag aaataaccca tagaggccat tttcatagta gggaagtatt cccatagata
     1021 ccggaaaaag caagatgccg gtgataagca gggctaatcc ggcaatcagc aaaatcacca
     1081 cttccagcgg aaaatgaact tctttcaata aagtatcttc catcttttta caccttcaat
     1141 gaacctttcc tggaattaat aataagttcc tttggtacgg tagttttcac gtcgttccgc
     1201 agcaagccca cggatcatct tatcaaactc tgccctattt tctctgtaaa gactttcagc
     1261 ttcacgcata ttttgcggaa agataataat catagatttc ttctctaatt cctgaaagat
     1321 atactctacg gcttcatcta ctgtgatagc atcaggcggt ggagtcatac taccgaatat
     1381 tgaagtgatg acattaccgg gacagaatac actgaattgt aaaccttcta cctctaattc
     1441 atattgcagg ctttcggtca tagatataac tgcactcttt gtggtagcat atactgcctg
     1501 gtagggaatg gggacacatc ctgctattga ggcagtgtta ataatatgtc caaaaccctg
     1561 ttctctcata attggaatag cgctataggt accatagata actccccata aattgaggtc
     1621 aatgagcatt ttccatatat caaaggttat ctgctcggta ggcaaggtca tacccatacc
     1681 ggcattgttg aagacaaaat caagatgtcc tcccaacatt ttggcctgtt taatgagttc
     1741 ttccacctgt tcaagcttgg taacatcagt caaaagcgga tgcaccttac cgggaaattc
     1801 agcatttaaa cgactggctt ctttatccag attctcctta ttaaaatcac ccataaatac
     1861 tgcttttgct cctcgcttaa gtaaatgctc gcttaatccc agacctattc ctgaggcagc
     1921 tcctgttacc actgccactt tatctttaaa atagtctgtc atattattct ccttttttaa
     1981 aataaagact taataaaata attaacgttt atttataatg ctcttgaatc tccaacattg
     2041 agccgggcag tagcttttat catgtttcca ccggtatact ttactatggg caactctgcc
     2101 agtgctctaa tgatatgtgc ctgcagggga tgttcctctg gtttaagtat gttccactca
     2161 agagttcctt ctcctgccca catcttctta acaactacct cctgggggta gagtgtcgca
     2221 tggctcagtg atgtaccacc tttgcccaga ttaggaagat atcgccagcc aaacagattg
     2281 attttggggt cttcattttt ccggtcaata gcactctgtg caagctcttc cttcctggta
     2341 aaaccaagtt ttaagaaggt attactttca tagcttgctg aggtaaacca gtaatcgccg
     2401 taatgacgtt cagaggatat atcggcaaat attttaggta caccatcctc ttcccgtcct
     2461 cccacaatag gacaggtcaa attttcccag accaccagcg cataatcacc ggtcagtcct
     2521 tcagaattac cggtatattt caccggggcg gttaactgaa tcagccgata ctctccacct
     2581 gccatccaat ccacatcccg acagttgtta tattgcaaag taataacagg ttcaatgagc
     2641 tcaaatacct cagggatata ctgtagcaac gcttcctgcg cagtctcata ttgtatgact
     2701 attcccgtgt tatccccgta tactgttctt accgggtaaa attctgaacc gccaaaatgg
     2761 accggcatct tgtagacaaa atcctctcta attttaaatt ttcctttcat aaaattctcc
     2821 tcttttaaaa atataataaa tcacataaac tgtgctggtc atttttagca ttttatttgg
     2881 taccattggt gccaaatgtg tttacaaaaa aaagagactc agctttcagc tattagtttt
     2941 cttcatcttc taatttttta ttgagtattt tgcttatcgt gttaaagtat ttacggacat
     3001 catctcacta agccatattt aaaa
//
LOCUS       DAOQWT010000158         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_35075_length_3001_cov_3.210455, whole genome shotgun sequence.
ACCESSION   DAOQWT010000158 DAOQWT010000000
VERSION     DAOQWT010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35075_length_3001_cov_3.210455"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PKJ95_03900"
     CDS             complement(<1..474)
                     /locus_tag="PKJ95_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR65423.1"
                     /translation="MSEVTYYFLGFAGIYVLMGWALYLPYRVGQLNFLIVACMAITAY
                     FGGFAAREWGWPFIVILIIGILFGATIAFIFSLAIGDAPCFTVVIVGLTSVFIVKTVI
                     ENWRTLGGTIGFFHIPGPNNMLAIIYSLLFLVGLLVFRIDHSRWGRAASVVFIDRD"
     gene            complement(512..1387)
                     /locus_tag="PKJ95_03905"
     CDS             complement(512..1387)
                     /locus_tag="PKJ95_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR65424.1"
                     /translation="MEIFIAQLISGLALGSIYVLLVTGFNLMLLVAKIIQFAYPQIVV
                     VSMYTCWWVLKLTGNKLIIILAGVSVSIILSLLSEPIFRKISSIEGVDINASFIASMG
                     IAMIMMDIMSHSINYGFPVAFSPDWVGGNIFGYGLITVSKGQLFALLGGIIAVLIFFF
                     LLYHTNQGRFFRAIAENRFAACLVGIPIIKTSIFSYLIVGILGGFSAILFSMLIGSAS
                     PWLGEFVALKMLAVSIVAGLGNLQGGLVCGLLLGIIEAMAIGYFSGSWSNVIAFAVML
                     IVVLMKPGGLFGIKI"
     gene            complement(1436..2155)
                     /locus_tag="PKJ95_03910"
     CDS             complement(1436..2155)
                     /locus_tag="PKJ95_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65425.1"
                     /translation="MEKINNILEICNLSIAYGPIKAVKEVNLEINHGEFVALIGANGA
                     GKSSLLKSILGINKAEKGSIFFLGQDITRKPTDKIVSSGLSLIPEGHVIFSSMSVLEN
                     LQLGAYQNLADFEENLKRIFDYFPVLKDRSSQIAGTLSGGEQQMLSMGRALMSKPKLI
                     LVDEPSLGLAPKIVTDIFNILTELNKEGYAILLAEQNAKKALEGSHRCYVMKTGEIAM
                     SGFSTDLIDDPKIRQAYLGGN"
     gene            complement(2139..2912)
                     /locus_tag="PKJ95_03915"
     CDS             complement(2139..2912)
                     /locus_tag="PKJ95_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65426.1"
                     /translation="MEEEMAILEVKNLCKSFGGLQALKNIDIEIQKNKIHGLIGPNGA
                     GKSTFFNVVSGLYPPTDGNIYFKSKEITNLPAEEITRMGISRTFQAGKLVPNLTVLDN
                     VMSGKYHISPNPVKSFFFQPSQFKNQEKDIKTSALEMLKFIGISDFAERWTSELVWVE
                     RQLVQIARALISSPDLLMLDEPTSGMGIDESKAVMDIIRRIRNMGITVIVVSHDMNLI
                     PEISDRITVLDFGNKISEGSPEQIQKDPRVLEAYLGENK"
BASE COUNT         1039 a          646 c          426 g          890 t
ORIGIN      
        1 atccctgtct ataaaaacaa ctgatgcagc ccttccccac ctggaatggt caatcctgaa
       61 taccagtaat ccaactaaaa acaataaact ataaataatg gcaagcatat tattggggcc
      121 agggatatgg aaaaagccaa tggtacctcc cagagttctc caattttcaa taactgtttt
      181 aacaataaaa actgatgtta ggccaacaat aacaactgtg aaacagggag catccccgat
      241 agccaaagaa aatataaagg caattgttgc accaaataat atcccaataa ttaaaatcac
      301 gataaatggc caaccccact ctcgcgcagc aaaaccacca aaatatgctg taattgccat
      361 acaagccact attagaaaat taagctgtcc tactctatat ggtaaataaa gtgcccatcc
      421 cattagaaca tagatgccgg caaaccctaa aaagtaatag gtaacctcag acaatattag
      481 cctccttatc aattgtttat cttaatatct cttatatttt gataccaaat aatcccccgg
      541 gcttcatcaa gactacaatt agcattaccg caaaagcaat aacattagac caggaaccgg
      601 aaaaatagcc tatagccata gcttcaatta ttcctaatag caggccacat accaatccac
      661 cctgtaaatt acctaatccc gctacaattg atacagctag catctttaaa gcaacaaatt
      721 cacctaacca gggagatgct gacccaatga gcattgaaaa tagtattgct gaaaatcccc
      781 ctaaaattcc aacaattaga taactaaaaa tactggtctt aataatggga atacctacca
      841 ggcaagcagc aaaacggttt tcagctatag ccctaaaaaa tctaccctga ttagtatgat
      901 acaggaggaa gaaaaatatc agcactgcaa taattccacc caataaggca aataactgcc
      961 cttttgatac agtaatcaat ccataaccaa aaatgtttcc acccacccag tcgggagaaa
     1021 aagcaacagg aaaaccatag ttaatagaat gagacataat gtccatcata atcatagcta
     1081 ttcccataga agcgataaac gaagcattaa tatctacacc ctcaatgctg ctaatcttcc
     1141 taaaaatagg ctcacttaat aaacttagaa ttattgaaac agatacaccc gccagaataa
     1201 taattaattt atttccggtt aattttaaaa cccaccaaca ggtgtacatt gataccacca
     1261 ctatctgggg atatgcaaat tgtataattt ttgctaccaa aagcattaaa ttgaatccgg
     1321 taaccagtaa tacataaata ctgcctaatg ccaatccact aattagctgg gctataaata
     1381 tttccatata ttcctctctt agaaattata caaaacaatt ttatcagttt attgattaat
     1441 ttccaccaag ataagcttgt ctaattttag gatcatctat taaatcggta gaaaatccag
     1501 acatagcaat ctcaccagtt ttcatcacat agcagcgatg gctcccctct aaagcttttt
     1561 tagcattctg ttctgctaaa agaatagcat acccttcctt gtttaactct gtaagaatat
     1621 taaatatgtc agtgactatt ttaggagcta aacccaggga gggctcatct accaatatta
     1681 gttttggttt ggacattaaa gctctaccca tagataacat ttgttgttcg ccaccactta
     1741 aggtccccgc tatttggcta cttcgatcct ttaaaacagg aaaataatca aatatccttt
     1801 ttaaattttc ttcaaaatct gcaaggttct gataagctcc cagttgaaga ttttctaaaa
     1861 cggacattga tgaaaaaata acatgccctt ctgggataag acttaagcca gagctaacta
     1921 tcttatccgt tggttttctg gtaatatcct gtcctaaaaa aaagatacta cctttctctg
     1981 ctttatttat acccaatatt gatttcaata aagaagattt cccggcacca tttgctccaa
     2041 tcagggctac aaattctcca tgatttattt ccaggttaac ctctttcact gcttttatag
     2101 gaccatatgc gattgaaagg ttacaaattt ctaaaatatt atttattttc tccaagatat
     2161 gcctccaaca ccctgggatc cttttgaatt tgttctggac taccttcgct aattttgttt
     2221 ccaaaatcta atactgtaat cctatctgaa atttcaggaa tcaggttcat atcatgacta
     2281 actacaatca cagtgattcc catgtttctt attcttctga taatatccat aaccgcttta
     2341 gactcatcta tacccatacc agaggtaggc tcatcaagca ttaacaaatc tggactggat
     2401 attaaagcac gggcaatctg taccaattgc cgttcgaccc agactagttc actggtccat
     2461 ctttcagcaa aatctgatat tccaataaat tttaacattt ccaaagcact ggttttaata
     2521 tctttctctt gatttttaaa ttgtgatggt tgaaaaaaga aggattttac aggattggga
     2581 ctgatatgat actttccaga cattacatta tccagaactg ttagattggg taccagtttc
     2641 cctgcctgaa aagttctgct tatacccatc ctagtgattt cttcagcagg aagattggtt
     2701 atttccttcg atttaaaata tatatttcca tcagtaggtg gatataatcc tgaaacaaca
     2761 ttaaaaaaag tagactttcc tgcaccattc gggccaatta aaccatgaat tttattcttc
     2821 tgaatttcaa tatcaatatt cttaagtgcc tgtaatccgc caaaagattt gcataaattt
     2881 tttacttcta atattgccat ttcctcttcc atcagggaaa ctctcctcta aactaaactg
     2941 tttcatatga tagtaaaaat caaattaggg gaagtggcaa acaaggaaga ggaacacatg
     3001 a
//
LOCUS       DAOQWT010000159         2999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_35104_length_2999_cov_4.382812, whole genome shotgun sequence.
ACCESSION   DAOQWT010000159 DAOQWT010000000
VERSION     DAOQWT010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35104_length_2999_cov_4.382812"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PKJ95_03920"
     CDS             complement(<1..379)
                     /locus_tag="PKJ95_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65427.1"
                     /translation="MGRTSKFTPEFKKKALILFRKGYSDREAFTELGISKNTFYRYLN
                     ENSDFSDQIQQVREKNVPHVENCMLKKCEGYFIEEEEILGRYEETKEPILDKDGKPTG
                     EFKIIKGMKPYTVKKTKKYIPPSD"
     gene            complement(385..1314)
                     /locus_tag="PKJ95_03925"
     CDS             complement(385..1314)
                     /locus_tag="PKJ95_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNR65428.1"
                     /translation="MNKTLYIPINSCYNENVTSGLNNRAFSKGFVLSPVRDRKGSKAE
                     SFRECLSYLRWIMGKKKNDNCGVYGIFNVHSEGKNKVYIGKSEDIERRWKDHKRLLNA
                     NKHGLNKSGQTDHLQRAWNNYGSESFEFHILKLCKSEELNHWEKILTWDHQSFNPFCG
                     YNRTPGGETNTMTTEAKEKLRQRMKGNKNMLGKTHTAEVRKILSEVNKGNKNMLGKHH
                     TKESNEKNRKWHLGRKCPESGIKISIANKGKKKPIRTEEHRKKLGQANIKYTHLEKEW
                     KELKKQGLFYRKISKIYNIPHQTIINYLKKSEV"
     gene            complement(1307..1429)
                     /locus_tag="PKJ95_03930"
     CDS             complement(1307..1429)
                     /locus_tag="PKJ95_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65429.1"
                     /translation="MKLSDLIFDNHNANLGTEKGTKLLESSLSKYGAGRSKINE"
     gene            complement(1434..1961)
                     /locus_tag="PKJ95_03935"
     CDS             complement(1434..1961)
                     /locus_tag="PKJ95_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNR65430.1"
                     /translation="MSYTVISTFAGCGGSSLGYKWAGFKELLAIEWEDNAVETFKLNF
                     PEVPIWQKDINNVTGKEILEFCNIKYGELDIFDFPVSNQKDVGNHKCPKPLKLFVDLI
                     ENYSEDNNTILDLFLGSGTTLIACEQTGRICYGIELEPLYIDVILRRYHKLYPDKEIQ
                     CLNREFPFDKLFKEG"
     gene            complement(1972..2511)
                     /locus_tag="PKJ95_03940"
     CDS             complement(1972..2511)
                     /locus_tag="PKJ95_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNR65431.1"
                     /translation="MQKIEDRVKQGKVKWKELIPIQNSNLKGFYEKEKEALLNSIKKY
                     GFIQSLNCWKDKGKIYTLDGVHRVDILKEIEKEFNIPEELPCNYIECKNRKEAAELVL
                     VYSSSYARVEHDGLIEFCEFEGLDFNDLKMDICLPEFSWDDFSMEGQGEKSDEDIKEK
                     ELDENIETDHECPSCGYKW"
     gene            complement(2499..2699)
                     /locus_tag="PKJ95_03945"
     CDS             complement(2499..2699)
                     /locus_tag="PKJ95_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65432.1"
                     /translation="MKFTEELKERSLKLLKAGYNNTEVCRDIDISLELFYTERKRDIQ
                     FKEKIEKAKMEFKERSDKECRR"
     gene            complement(2798..>2999)
                     /locus_tag="PKJ95_03950"
     CDS             complement(2798..>2999)
                     /locus_tag="PKJ95_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65433.1"
                     /translation="IRKYHVTTGESTFSHLILYLIKENNEMRSKISGIEFKVNQLISL
                     KEKGEVKEKEKKVKKPIVKNKP"
BASE COUNT          810 a          607 c          350 g         1232 t
ORIGIN      
        1 ggtctgaagg tggaatgtat ttctttgttt ttttaactgt ataaggtttc attcctttaa
       61 ttatcttgaa ttcaccagtg ggtttaccgt ctttatctaa tatgggttct tttgtttcct
      121 cgtatctacc caatatttcc tcttcttcaa tgaaataacc ttcacacttt ttaagcatac
      181 aattttctac gtgagggacg tttttttctc taacctgttg aatttggtca ctaaagtcac
      241 tattttcgtt taaataacga taaaatgtat ttttggaaat acctaattca gtaaaagcct
      301 ctctatcaga ataacccttt ctgaacaaga ttaaagcctt ctttttaaac tccggtgtaa
      361 attttgatgt cctacccatt tcgtctatac ctcacttttt tttaaatagt taataatagt
      421 ttgatgtggt atattatata tttttgatat tttacgataa aacaacccct gtttttttaa
      481 ttctttccac tctttttcta gatgtgtata ttttatattg gcctgtccta atttctttct
      541 atgttcttct gttcttatag gtttcttttt tcctttattt gctatactta tttttattcc
      601 tgattctggg cattttcttc ctaaatgcca ttttctattt ttctcattag attcttttgt
      661 atgatgcttc cctaacatat ttttatttcc tttatttact tcacttaata tttttcttac
      721 ttctgcggta tgagttttac ctaacatatt tttattaccc ttcattcttt gtcgaagttt
      781 ttcttttgcc tctgttgtca tagtattagt ttctcctcca ggagttctat tgtaaccaca
      841 aaaaggatta aaactttgat gatcccatgt taaaatcttt tcccagtgat ttaattcttc
      901 tgatttacat aattttaaaa tgtgaaactc aaaactttca gaaccgtaat tattccaggc
      961 tctctgaaga tgatctgttt gtcctgattt atttaaaccg tgtttatttg catttaaaag
     1021 tcttttatga tctttccatc ttctttctat atcttcactt ttccctatat atactttatt
     1081 ctttccttca gaatgaacat tgaatatccc atacacacca cagttatcat tcttcttttt
     1141 tcccataatc cacctcaaat aactaaggca ctctctaaag gactctgctt tgcttccttt
     1201 tctgtcacga acaggtgaaa gcacaaatcc tttagaaaat gccctattat ttaacccgct
     1261 cgtgacattt tcattataac atgaattaat gggaatataa agagttttat tcatttattt
     1321 tacttcttcc tgccccgtat ttactcagtg aactttcaag aagtttagtc cctttttcag
     1381 ttccaagatt ggcattatga ttgtcaaaga ttaaatctga aagtttcatt tatttacccc
     1441 tctttaaaaa gtttatcgaa aggaaattct ctatttaagc actgtatttc tttatcggga
     1501 tagagtttat ggtatcttct taagattacg tcaatataca ggggttcgag ttcgattccg
     1561 taacatatcc tccctgtctg ctcacaggcg attagggtag tgcctgagcc gaggaagagg
     1621 tcgaggattg tgttattatc ttctgaatag ttttcaatta aatcaacgaa taattttaaa
     1681 ggctttgggc atttatggtt acctacatct ttttgattac ttacggggaa atcgaatata
     1741 tctaattccc catactttat attacaaaat tccaagatct cttttcctgt gacattatta
     1801 atatctttct gccatatagg aacttcaggg aaatttaatt taaaagtttc aactgcatta
     1861 tcttcccatt caatagcaag taattcttta aatcctgccc acttataacc taaagaactt
     1921 ccaccacaac cggcaaaagt tgaaataact gtataactca taaattactc cttaccattt
     1981 atatccacag gaaggacatt catgatctgt ttctatattt tcatccaatt ctttttcttt
     2041 aatatcttca tctgatttct ctccctgtcc ttccatgctg aaatcatccc aactaaactc
     2101 aggtaagcaa atatccattt tcagatcatt aaaatccaaa ccctcaaatt cacagaattc
     2161 aatcagtccg tcatgttcca cacgggcata agaactgctg taaaccaaga ctaactcggc
     2221 ggcttctttt ctgtttttac actctatata attacagggt aattcttccg ggatattaaa
     2281 ttccttctct atttctttga gtatatccac ccggtgcact ccgtctaaag tataaatttt
     2341 ccctttatcc ttccaacagt ttaagctctg aataaatcca tacttcttta tactgtttaa
     2401 cagggcttcc ttttcttttt cataaaatcc tttcagattt gaattctgta tggggattaa
     2461 ctctttccat tttaccttac cctgttttac cctatcttct atcttctgca ctctttatca
     2521 cttctttctt taaattccat ttttgccttt tctatcttct ctttgaattg aatatctctt
     2581 ttcctctcag tatagaataa ttctaatgaa atatcaatat cccggcaaac ttctgtatta
     2641 ttatatcctg cctttaaaag ttttaaagat ctctctttta attcttcagt aaatttcata
     2701 acatacttcc tatgcttcct atatcgctca tgaatgaagc aaattgattt tttatactct
     2761 tttactttca aaacctatcc tactttaaaa cgcaaaatta gggcttattt ttaactatag
     2821 gttttttaac cttcttctcc ttttctttta cctccccttt ttcttttaaa gagattaact
     2881 gattaacctt aaattctatt cctgaaatct tcgacctcat ttcattattt tcttttatca
     2941 gataaaggat taaatggctg aaagtagact ctcccgtagt cacatggtat tttctaata
//
LOCUS       DAOQWT010000160         2978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_35432_length_2978_cov_3.437906, whole genome shotgun sequence.
ACCESSION   DAOQWT010000160 DAOQWT010000000
VERSION     DAOQWT010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35432_length_2978_cov_3.437906"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PKJ95_03955"
     CDS             <1..1047
                     /locus_tag="PKJ95_03955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR65434.1"
                     /translation="YVVIESTYGGRVNRPYRQEVKKFFSIIDKTLKRGGDVIIPAFAL
                     QRTQDIIYELSQYYNLQLKQLVHRDKKLKKLRFYLDSPLAIAVTKIYRNNPQDFAQKD
                     LRIMENRNQLLDFQSLRMTSTANASKQINRSRRSKIIISASGMCDGGRIQYHLKEHLW
                     KKESSVIFVGYQAEGTLGRKIVSGEKCLDILDEMVDVKAEIYHLDGFSSHADRDELIW
                     WLKGLKNKPKKVFIVHGEKEESESLAMNLDEQLDFDAYIPVMGETFLIKDKQITSQGK
                     IKDSSKELQLDEILTDLNKIKHKFHLLLTEMELFGLENEQESLWYTIKGKVEELQKDI
                     RRVKYLIKENELEV"
     gene            1128..1913
                     /locus_tag="PKJ95_03960"
     CDS             1128..1913
                     /locus_tag="PKJ95_03960"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNR65435.1"
                     /translation="MNYDVLESIISVALNAGNYIRHYYEGRGFKVSEKKDSHDLVTDI
                     DRESQRIITTELSRRFPDISIIGEEDQDVVKNENAFFVDPLDGTLNFVKQIPFFTVSI
                     GYWKDNKPVCAVVFDPIRKDLFYARKGSGSYRNGRKLEITDSNNGKYQLLASDWGHEP
                     YYYQKNILTIQQLLKENLYLFRFMGCASLAICYVAAGILDGYWHYKLSPWDMAAGVLI
                     AREAGASVTSVKGKSFDLWQKDILVAEPNIKNKIIPVLSQIEI"
     gene            2014..>2978
                     /locus_tag="PKJ95_03965"
     CDS             2014..>2978
                     /locus_tag="PKJ95_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNR65436.1"
                     /translation="MGQDSNRKSKKISKSRIPRKFRWDIVSLYKSGKEWQKDCSKLEE
                     LLNRLSELKYRFTEESGLFLEVLQLKDEILQLAQRIYTYAHMCKDEDNSNNLYQSFFY
                     QASSLSVKAEKSLSFIEPAILSLDYNQLKKWLQDKKELQVYERCLEKIYRKKEHTLTA
                     EEEKIIAQTGDMAYMFEYSFELLSYADLSFPKIKDEGGRIVPITQSNFSTLLRDKNRE
                     VRKRAFKSYYKVYQKHRNIYAALLAGNIKKDQFYSSTRKYNNSLEAALFSEKIPREVY
                     TGLLDFVHKKINLLHKYTQLKRDYLGLDQLHMYDMYAPLAEGTSK"
BASE COUNT         1044 a          464 c          610 g          860 t
ORIGIN      
        1 tatgttgtaa ttgaatctac ttatggtggc agagtcaacc gtccttatcg ccaggaagta
       61 aaaaagtttt tttccatcat tgataaaacc ttaaaaaggg gaggagatgt cattattcct
      121 gcctttgctc tacagagaac gcaggatatt atttatgagt taagtcagta ttacaattta
      181 cagctgaaac aattggtaca tagggataaa aaactaaaaa agctgagatt ctatctggac
      241 agccccctgg ctattgctgt aaccaagatt tatcgtaata atccccagga tttcgcccag
      301 aaagatttaa gaataatgga gaataggaat caattgcttg attttcaatc tttgagaatg
      361 accagtactg caaatgcatc caaacagata aatcgttcca gaagaagtaa aataataatc
      421 tctgccagtg gtatgtgtga cggaggtaga attcaatatc acttgaagga acacctctgg
      481 aaaaaagaat cttctgttat attcgtggga tatcaggcag agggaacact gggtagaaag
      541 atagtcagtg gggaaaaatg tttagatata ttggatgaga tggttgatgt gaaggcagaa
      601 atttatcatc ttgatggttt ttcttctcat gcagataggg atgaactaat atggtggctg
      661 aaagggttga aaaataaacc aaaaaaagtt tttatagtac atggagaaaa agaagaatca
      721 gaatcccttg ccatgaacct ggatgaacag ctggattttg atgcctacat acctgttatg
      781 ggcgaaacct tcctcattaa agataaacaa attacttcac aggggaagat aaaagatagc
      841 agtaaggagt tacagctgga tgaaattctc accgatttga ataaaattaa acataaattt
      901 catctcttat tgactgaaat ggaactattt ggtttagaaa atgaacagga gagcctgtgg
      961 tatacaataa agggaaaggt agaggagtta cagaaagata ttcgcagagt gaagtactta
     1021 ataaaagaga atgaactgga agtttgatac ggagagtata aataacagtt aaaagtctta
     1081 taccactctg tttttaaaaa agatatgaaa tttgtgcagg gaaagatatg aattatgatg
     1141 tactggaaag cataattagt gttgccctga atgccggtaa ttatattaga cattattatg
     1201 aaggcagggg ttttaaagta tcagaaaaaa aggatagcca tgatctggta acagacattg
     1261 accgggaatc acaaagaata attactacag aactttcccg tagattccct gatatttcca
     1321 taataggaga agaagatcag gatgtcgtaa aaaacgagaa tgcttttttt gttgaccctc
     1381 tagacggcac tttaaatttt gtaaaacaaa ttcctttttt tacggtatcc attggttact
     1441 ggaaagataa taagccggtt tgtgctgtgg tctttgaccc aatcaggaaa gatctttttt
     1501 atgcccgtaa aggaagcggt tcttatcgta atggaaggaa attagaaatt accgattcta
     1561 ataatgggaa gtatcagtta ttagcctcgg actggggtca cgaaccatac tactaccaaa
     1621 aaaacattct tactattcag caattactaa aagaaaatct ttatttattc aggtttatgg
     1681 gttgtgccag tctggcgata tgttatgtag ccgcagggat attggatggt tactggcatt
     1741 ataaactttc tccatgggat atggctgcgg gtgtgctaat agcccgggaa gcaggggcca
     1801 gtgttacctc tgttaaggga aaatcgttcg acctctggca gaaagatata ctggtagcag
     1861 agcccaatat aaagaacaaa attattccag tattaagcca gatagagatt taaaagtatt
     1921 cactgctatt aaatattttt atctaatctg acagggtatc gaaatatcaa ttaccaatca
     1981 tcttatcatt tttaagattc attaaaggat tttatggggc aggattccaa caggaaaagt
     2041 aaaaaaatat ctaaaagccg aatccccagg aagtttcgct gggatatcgt gtccctttat
     2101 aaaagtggga aagaatggca aaaggattgc tctaagttag aagaactgct caacaggctg
     2161 tcagaactca aatatcgttt taccgaagaa tctggactct ttctggaagt tctgcaattg
     2221 aaggacgaaa tcttacagtt ggctcaaaga atttatacct atgcccatat gtgtaaagat
     2281 gaagataata gtaataatct ctaccaatca ttcttctacc aggcttcttc cttatcagtt
     2341 aaagcagaga aatccctttc ttttattgaa ccggcaatcc tatctttaga ttataatcaa
     2401 ctaaaaaaat ggttgcagga caaaaaggaa ttgcaggttt atgaacgatg cctggaaaaa
     2461 atctatagaa agaaagagca tactcttact gctgaggaag agaagattat tgcccaaaca
     2521 ggcgatatgg cctatatgtt cgagtactca tttgaattat tgagttatgc tgatcttagt
     2581 tttcccaaga tcaaggatga ggggggaaga attgttccca taacccagag taatttttct
     2641 accttgctca gagataaaaa tagagaagtg cgcaaaagag catttaagag ttattacaaa
     2701 gtttatcaaa aacatcgcaa tatttatgct gccttgctgg ctggaaacat aaaaaaagat
     2761 caattttata gctctaccag aaaatacaat aatagcctgg aagctgctct ttttagcgaa
     2821 aaaattccca gagaggttta taccggttta ttggattttg tccataaaaa aattaatctt
     2881 ttgcacaaat atacacagct aaaacgtgat tatttaggct tggaccagct acatatgtat
     2941 gatatgtatg cacctttagc tgaaggaacc tcaaaaat
//
LOCUS       DAOQWT010000161         2969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_35594_length_2969_cov_4.216884, whole genome shotgun sequence.
ACCESSION   DAOQWT010000161 DAOQWT010000000
VERSION     DAOQWT010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35594_length_2969_cov_4.216884"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKJ95_03970"
     CDS             <1..252
                     /locus_tag="PKJ95_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004860245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNR65437.1"
                     /translation="ELAEKVIKYYSFINDVILDPFAGLGTVAEAAYKLKRRFVVLENN
                     PEYVKLIKDNLEKCLKNDISYVNWLGCESVIQKEPYFDI"
     gene            267..1361
                     /locus_tag="PKJ95_03975"
     CDS             267..1361
                     /locus_tag="PKJ95_03975"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfrBI family restriction endonuclease"
                     /protein_id="HNR65438.1"
                     /translation="MSQKITSFIPPMGVSLAKYTGKQVVERIGEVIIKNAVTSVLCGE
                     NVRSYTEEFTRRRLTLCNASLFMTFLKSVSNIDNFLENMPSIIKEELLIKKIPEGKRV
                     FLEWFIGLTGKSIQNVLRDKESGTEEMDEYLKTYQKTINDAMENCKIDFGNIEGHLNW
                     KDINCNVNWKFFLYLFSAIGAQTLAIRGSEKSLYGKLFEKLVLASFLTILGFKMIDKQ
                     DISKSEKVFWLSSKDNDKRESDATLLYKPGVGVRFDIGFIGRGNPEISLDKVSRFERE
                     MEHGRQKYYMSTIIIVDKIGEGSRIVNLAKNIKGNIVQMSMAYWVKEICKILEEKINF
                     KHKLLTLSNEQTLDYIKEEIQNINLNNFVK"
     gene            complement(1556..2491)
                     /locus_tag="PKJ95_03980"
     CDS             complement(1556..2491)
                     /locus_tag="PKJ95_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR65439.1"
                     /translation="MNILVTGSTGFIGKRLVELLLLQNYNVRVLLRETSNIDKIDKRV
                     KIFQGNLFDDSFLKSSMKDIDIVFHLAALRGETGLTWKAYEKSNILLTEKLLEASRNV
                     KRFIFCSTVGVMGFGTGLSEKSPLNPEGYYHISKSEAEKLCLNYENCIIVRPSIVYGP
                     GDNGFLYKLINLIKKKRFIIVGNGKNKIHMVHIDNLCKGFINTALNGNLREIYIIADS
                     KALTVSEMSQVISKKLGVNIPSVTVPGIIALPLAFLFQKCFSIVFPGKEPFISVPKVN
                     ILSLNQDFDTSKVLSIGYNPDIEPEKGIGGMTDLC"
     gene            complement(2560..2703)
                     /locus_tag="PKJ95_03985"
     CDS             complement(2560..2703)
                     /locus_tag="PKJ95_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65440.1"
                     /translation="MTVEIYRKIVKGKELKNFIELPDDYLDKEMEVIIKPVVKKYLIL
                     YQL"
BASE COUNT         1084 a          386 c          489 g         1010 t
ORIGIN      
        1 gaattggcag aaaaagttat aaaatattat tcttttataa atgatgttat tttagacccc
       61 tttgcagggt taggaactgt cgcagaagca gcatataaat taaaacgacg ttttgttgta
      121 ttagaaaaca acccagaata tgttaaatta ataaaagata atttagagaa atgtttaaag
      181 aatgatattt catatgtaaa ttggttaggg tgcgaatcgg taatacaaaa agaaccatat
      241 tttgatatat aagaaaagag gtatatatga gtcaaaaaat aacatccttt attcctccta
      301 tgggagtctc gttagcaaaa tacactggaa aacaggtagt cgagcgtata ggtgaagtaa
      361 taataaaaaa tgcagttact tctgttttat gtggtgaaaa tgtcaggtcg tatacggagg
      421 aatttacaag aagaagattg actttatgca atgcatcatt attcatgact ttcttaaaat
      481 ctgttagtaa tattgataat tttttagaga atatgccgtc aataatcaag gaagaactat
      541 taataaaaaa aatacctgaa gggaagagag tttttttaga atggtttatc ggtttaactg
      601 gtaaaagtat acaaaatgtt ttaagagata aagaatctgg aacagaggaa atggatgaat
      661 atctaaagac ttatcaaaaa actataaatg atgctatgga aaattgtaag atagatttcg
      721 gaaatattga aggtcattta aattggaaag atattaattg taatgttaat tggaaatttt
      781 ttttatattt attttctgct attggtgctc aaaccttagc aataagaggt tctgaaaaat
      841 ctctctatgg aaagttgttt gaaaaattag tattagcatc tttcctgaca attttgggtt
      901 ttaaaatgat agataaacaa gatatatcaa aatcagaaaa agttttttgg ctttcatcta
      961 aagataatga taaaagagaa agtgatgcta cgttacttta taaaccaggt gtgggggttc
     1021 gttttgatat aggatttata ggtagaggta accctgaaat atctttagat aaagtgtcta
     1081 gatttgaaag agaaatggaa catggcagac aaaaatatta tatgtcaacc atcattattg
     1141 tagataaaat aggagaagga agtagaatag ttaatttagc taaaaatata aaaggaaata
     1201 ttgtacaaat gagtatggca tattgggtga aagaaatttg taagatatta gaggagaaaa
     1261 ttaattttaa acataaatta ctaactttat caaatgagca aacattagat tacataaaag
     1321 aagaaattca aaatattaat ttaaataatt ttgttaaata aaatacttat tattatttaa
     1381 tttctgggtg atctggggga aacaggtgga atgctaagcg gggatatagg gtatttttaa
     1441 acaaacaggg taaatatacc atgagaacat cgtgaaaact taatatttac aacagtttta
     1501 tttaatttta cgtaaataaa aggatttata cgaaaagata tagaaattac tctatttagc
     1561 aaagatcagt catcccccct atgccctttt ccggctcaat atccggatta tatccaatag
     1621 ataaaacttt tgatgtatca aaatcctgat tgagacttaa tatattcact tttgggacag
     1681 aaataaaagg ttcttttccg ggaaatacta tggaaaaaca tttctgaaaa aggaaggcca
     1741 gaggtaaagc tattattccc ggaacagtaa cagatggaat atttactcca agctttttcg
     1801 atataacctg actcatctct gaaacagtga gggctttact gtctgcaatt atatatattt
     1861 ctcttaaatt accattgaga gctgtattaa taaaaccttt acagagatta tctatgtgaa
     1921 ccatatgaat tttattttta ccatttccaa cgataatgaa tcttttcttt tttatcagat
     1981 tgataagttt atagagaaaa ccgttatccc ccggtccgta gactatggaa ggtcttacta
     2041 tgatgcagtt ttcataatta agacagagtt tttctgcttc acttttagaa atatgataat
     2101 agccttcagg atttaaagga gatttttcgg aaagtcctgt tccgaagccc ataactccta
     2161 ctgtactgca aaaaataaat cttttaacat tacgtgaagc ttccaggagt ttttctgtca
     2221 ggagaatatt ggatttttca tatgctttcc aggtaagacc tgtttctccc cttaaagcgg
     2281 caagatgaaa tactatatct atatctttca tggaggattt gagaaaagaa tcatcaaata
     2341 aattgccctg aaatattttt acccttttat ctattttatc tatatttgag gtctccctga
     2401 gaagaactct tacattgtaa ttctgaagga gaagtaattc tacaagtctt ttgcctataa
     2461 agcctgtgga accggtgaca agtatattca tgtatctata aagtttctat ctttcattgg
     2521 cttcatctct gttaaacctg aattcctctg tattaatttt tataattgat agagaattaa
     2581 atattttttt actacaggtt taattattac ttccatttct ttatctaaat aatcatcagg
     2641 aagttctatg aaatttttta attccttacc ttttacaatt tttctataaa tttcaactgt
     2701 cataagaatt tccctttcta tttaaaagtt tcactaaatc tattttacca tagaagggta
     2761 tttttaaaca aacagtataa atataccatg ggaaaatcgt aaaaacttag tatttagccc
     2821 agtaagagca aacgtgtcac caggggcaaa gtataagaaa ttttaaaaaa aagattattt
     2881 gctcctgaat tttacaggta attttccttc aattaaggcc ctggctattt tcctatattc
     2941 caactttgtt taactgaaaa gaaataacc
//
LOCUS       DAOQWT010000162         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_35709_length_2961_cov_3.305919, whole genome shotgun sequence.
ACCESSION   DAOQWT010000162 DAOQWT010000000
VERSION     DAOQWT010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35709_length_2961_cov_3.305919"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKJ95_03990"
     CDS             complement(<1..214)
                     /locus_tag="PKJ95_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNR65441.1"
                     /translation="MNKVESIRMTNGLRLITCPMPHMQSVSIVIGVKTGSIYERKEEQ
                     GISHFIEHMLFKGTEKRKNTLEISQAI"
     gene            complement(236..1066)
                     /gene="mreC"
                     /locus_tag="PKJ95_03995"
     CDS             complement(236..1066)
                     /gene="mreC"
                     /locus_tag="PKJ95_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNR65442.1"
                     /translation="MKKLSKERKAFAVFLFLSLLSALLITVDYHGKGFLGLLKDTGFL
                     ILKPVNKIVQNTQRHFVKYYQMFADVESIIEKSGKLEQENQELLQENAILKEKLTAYE
                     RIAKMVQFKEYYNYEMIPAQVIGREPDNWFHSIIVDMGSKDGIEIDMGVATHKGLVGK
                     VIQVERNTSEVLLLIDQGCSVGAMVQRPREVGVIKGATESVYCYLDYVAHDADVQLND
                     VVVTSGMGSTIPKGINIGRVVVIKKEKHDLFQRILIKPDADFNKLEEVFIVKSPDYEK
                     "
     gene            complement(1174..2226)
                     /locus_tag="PKJ95_04000"
     CDS             complement(1174..2226)
                     /locus_tag="PKJ95_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNR65443.1"
                     /translation="MFNLSFSLSLSRNIGIDLGTSTTLIYVKGKGIILEEPSIVAYQK
                     NDNRVLKVGKEAKFMIGRTPKGVYTIRPLQYGVIANFEVTAEMLRYFIKQIFSRYQFI
                     KPYITICVPSGVSEVEKRAVSEVAYECGSRRVFLIEEPIAAAIGAGLPVFEPMGNLIV
                     DIGGGTSEVAVISLGGIVVSEITKVAGDYLNHQIIKYVKENRGLFIGDLTAEELKISL
                     SKRNDISEEDYKEDSFEARGRDRLTGLPIKIELTKNELKEALYDSVVEIASTVKLVLE
                     KTPPELISDIINKGLIMTGGGSLLSDLDKFIQEQIKIPVFVSKEPQYCVAKGTGEALE
                     NFDSHSRVLIKTSRSR"
     assembly_gap    2308..2317
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2448..>2961)
                     /gene="amrA"
                     /locus_tag="PKJ95_04005"
     CDS             complement(2448..>2961)
                     /gene="amrA"
                     /locus_tag="PKJ95_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNR65444.1"
                     /translation="NESPPVVLAKKSIIYYLQNRKKLPLPADLPKELIKEQAGVFVSL
                     KKEGRLRGCIGTFLPTKSNTALEIIENAVSAAIRDPRFCPVTLDEVESLSISVDILTA
                     PEKVKDISQLDPRKYGVIVSSGYKKGLLLPDLEGVDSAEQQIDIARQKAGIYTDEDFF
                     IERFEVKRYY"
BASE COUNT          874 a          636 c          447 g          994 t
ORIGIN      
        1 caatagcctg ggatatttct aaagtattct tcctcttttc agtacctttg aagagcatat
       61 gttcaatgaa atgagaaata ccctgctctt cttttctttc atagatagaa ccggttttta
      121 ctcctatcac tatggagaca gattgcatat gtggcatagg gcaagtaata agtcgcagac
      181 cattagtcat tcttatcgat tcaactttat tcataaatca gaatttactc ctcattcatt
      241 tctcatagtc aggggatttg acgataaaga cctcttctaa tttgttaaag tcagcatccg
      301 gtttgattaa aattctttga aaaaggtcat gtttctcctt tttaataacc acaacccgcc
      361 caatattaat tccttttgga atagtactac ccatacctga ggtaaccaca acatcattga
      421 gttggacatc agcatcgtgt gcaacataat caagataaca atagacactt tcggtagctc
      481 cctttattac ccccacttct ctcggtctct gaaccatagc acctactgaa cagccctgat
      541 cgattaacag aaggacctct gaggtatttc tttccacctg aataactttt cccaccaggc
      601 ccttgtgggt agccactccc atatcaatct caataccatc tttactcccc atatctacaa
      661 ttatgctgtg aaaccaattg tctggttccc tgccaattac ctgagccggt atcatctcat
      721 aattataata ttctttaaac tgcaccattt ttgctatccg ttcataggca gtcagctttt
      781 ctttaagaat agcgttctct tgtaaaagct cttggttttc ttgctctaat ttcccgcttt
      841 tttcaataat gctttccaca tcagcaaaca tctgatagta ttttacaaaa tggcgctggg
      901 tattctgaac aatcttattt actggtttta gtattaaaaa gcctgtgtct ttcaataatc
      961 ctaaaaaccc cttaccatgg taatcaacag taataagcaa agctgataat aaggaaagga
     1021 ataaaaacac agcaaaggct tttctctctt tagaaagctt tttcaataaa aacactcccc
     1081 actcctattt taaactctgt aaaaatacaa tgaacagtac atcgtacaga agtatctttg
     1141 caaattaaaa acaccttatt tattattttc ccatcacctg ctcctggaag tctttatgag
     1201 aaccctacta tgactatcaa agttttccag tgcttctcct gttcctttgg ctacacagta
     1261 ttgcggttct ttggacacaa aaacagggat ttttatttgc tcctgaataa atttatctaa
     1321 atctgacaaa agagaccctc ctccggtcat aattaaccct ttattgataa tatcagaaat
     1381 taattcagga ggggtcttct ctaaaaccag cttcactgtg ctggctattt ccacaactga
     1441 atcatagaga gcttctttta attcattttt ggttagttct attttaatag gcagtccagt
     1501 gagtcggtcc ctgccccttg cttcaaaaga atcttcttta taatcctctt cacttatatc
     1561 gtttcgttta gaaagagata tctttaactc ttccgcagtt agatctccaa tgaaaagacc
     1621 acggttttcc ttgacatatt tgattatctg atggttcaaa taatcaccgg caaccttagt
     1681 tatttcactt accacgattc ctcccaaaga gataaccgcc acttctgaag tacctccgcc
     1741 aatatctaca atcagattgc ccatgggctc aaaaacaggc aatccggcac caatagccgc
     1801 cgcaatgggt tcttcaatca aaaatactct tctcgaacca cattcataag caacttcaga
     1861 aactgctctc ttttcaactt cagaaacacc cgagggaaca caaatagtga tgtagggttt
     1921 gataaactga taacgggaaa atatttgttt tataaaatag cggagcattt cagcagtcac
     1981 ttcaaaattg gcaatcacac catactgcaa tggacgaatg gtgtaaacac cttttggtgt
     2041 tctgccaatc ataaatttgg cttctttccc cacttttaaa actcgattgt catttttttg
     2101 atatgccact atggaaggtt cttctaaaat aattcccttt cctttaacat atatcagagt
     2161 ggtactggtg ccaaggtcta taccaatgtt gcgggaaagt gatagagaaa atgaaaggtt
     2221 aaacatttat tgagcccctt ttggttaata tttttaaatt ataagaattt tttagtactt
     2281 ccgttttaat aaagaatata aaattatnnn nnnnnnnttt tggttaatat tttttgaatt
     2341 ataaggattt tttaatattt ccgtatttaa agaatataga atatatgata tcatttttgt
     2401 gaaattgtaa aaatattaaa ttttttacat taagtaattc tctgtattca gtaatatctt
     2461 tttacttcaa atctttcaat gaaaaaatct tcgtctgtat atatccctgc tttttgcctg
     2521 gcgatatcta tctgctgttc tgcactatct actccttcca ggtcaggcaa caaaagacct
     2581 ttcttataac ctgaactgac tatcaccccg tacttcctgg ggtcgagctg gctgatatct
     2641 ttcacttttt ccggagcagt aagaatatca actgatatgg acaggctttc cacttcatcc
     2701 agagtaaccg gacaaaacct ggggtcacgt atagctgcac ttacagcatt ttctataatc
     2761 tccaaagcag tattactctt tgtgggaagg aaggtaccaa tgcagcccct cagtcttcct
     2821 tcttttttta aagaaacgaa tacccctgcc tgttccttga tcaattcttt gggcagatca
     2881 gcaggaagag gaagtttttt tctgttttgc aaataataga taatgctttt tttagccaat
     2941 actaccgggg gactctcatt t
//
LOCUS       DAOQWT010000163         2941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_36074_length_2941_cov_2.198891, whole genome shotgun sequence.
ACCESSION   DAOQWT010000163 DAOQWT010000000
VERSION     DAOQWT010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36074_length_2941_cov_2.198891"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKJ95_04010"
     CDS             <1..308
                     /locus_tag="PKJ95_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65445.1"
                     /translation="QNALKDTTKMIEEKNGNYIFLKRMTDKLKRFNYLLSMKLFGRVI
                     TDVKEMESERKREAEIFKKALIEELGKTEPKMREKIVRRIHRITVKALERPGEEVNG"
     gene            301..951
                     /locus_tag="PKJ95_04015"
     CDS             301..951
                     /locus_tag="PKJ95_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65446.1"
                     /translation="MDNGKTEKKKSKQKKKNQDTVNIKKMPELGKTEKKLKFVELRAK
                     GLSYRQIEQDLHIGRSACGEWNEELKEEIVKRKAEYLEELYHNYYMLREARIEKLGEI
                     FKKLDAELANRDITDLTTKELLDYYLKYLSELKEEYIDLKNTKSRLKSDSQDILREFS
                     DLLNRVRAGEVTKDQAVKENYVLANLLKAYEILIVEKKIDSIKSIIGGRPDEKVKF"
     gene            932..1660
                     /locus_tag="PKJ95_04020"
     CDS             932..1660
                     /locus_tag="PKJ95_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65447.1"
                     /translation="MRKLNFNNIVKDKLTGHELGKLLIKDCITGIISAYNDKDIMAGT
                     KPEEHILDTFEINNLIDGLTGTYNIQIYKDYREIWKFLSDISVMQMIYAYVIEIAFWK
                     LTSAISHHDTCPLEDDEIDGMIKAINETNENIKKFFLLEAIKELIARYTQVYEILQTM
                     TAFPEDLFLYANMLIDKYNVLYKQADILPIVKMCPITEEKIQEVLGKINEVSTLYNMK
                     KLEDLLLSENKETCIVGGKEDEEY"
     gene            1647..2357
                     /locus_tag="PKJ95_04025"
     CDS             1647..2357
                     /locus_tag="PKJ95_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65448.1"
                     /translation="MKNIDLMSMTKGKLSGEEMGKLIMKHYFTDLLEGEKNNPIPFPE
                     LKRIVNTLQGSRNIEIYKQYEKLLQFLVQESIRMDDYRLKMELMLWKVSFLLTYSLTS
                     KSKPFEPKEIEGLTIMIYSINDMIKQFFFLQVIMELIIKITKIEEIYCFFSTFPVRLI
                     KATNKLIEITEESLPLIEYNVMTPETFKKALAGVGDISDLQDEEKMRGLLGIPESLNI
                     PVLSEGRTAISYEELAKL"
     gene            2669..>2941
                     /locus_tag="PKJ95_04030"
     CDS             2669..>2941
                     /locus_tag="PKJ95_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65449.1"
                     /translation="MVEKEITIPKEVYEQLPWALELCLTDPLIEKDKESLNGFYGMYQ
                     KIEYYHGDKYKHLLDKINSLKYGKEAIEEATEEQLRSTFEVYISRSK"
BASE COUNT         1169 a          394 c          594 g          784 t
ORIGIN      
        1 tgcaaaatgc cttgaaagac actacaaaaa tgatagaaga aaagaatgga aactatatat
       61 tccttaagag gatgacagat aaattaaaac gtttcaatta ccttctgtct atgaagcttt
      121 tcggtagagt aattacagac gttaaggaga tggaatcaga acgcaaaagg gaagcagaaa
      181 tctttaaaaa agctcttatc gaagaacttg gaaaaacgga gccaaaaatg agagaaaaaa
      241 tagtaagaag aatacacaga attacagtga aagcacttga aaggccgggt gaagaagtaa
      301 atggataatg gcaagacgga gaaaaagaag agtaagcaga agaaaaagaa tcaggacaca
      361 gtaaatataa agaagatgcc tgaactggga aagactgaga agaagttaaa atttgtagaa
      421 ctcagagcca aggggctgtc ataccggcag attgaacagg atttacatat aggaagatct
      481 gcctgtggtg agtggaacga agaattaaaa gaagagatag taaagcgtaa agcggaatat
      541 ctggaagaac tttaccataa ttactatatg ttacgtgagg cccggattga aaaacttgga
      601 gaaattttca agaaactgga tgcagaactt gccaatagag atataacaga ccttaccaca
      661 aaagaacttc ttgactatta tctaaagtat ttatccgagt tgaaggaaga gtatatagac
      721 ctgaaaaaca ccaaaagtag gctaaaatcg gacagtcagg acattttgag agaattttct
      781 gatttattga accgagtaag ggccggcgaa gtaacaaaag accaggcagt aaaagaaaac
      841 tatgttcttg caaatctttt gaaagcttat gaaatcttaa tcgtagaaaa gaaaattgac
      901 tcaataaaat caataatagg agggcgacca gatgagaaag ttaaatttta acaatattgt
      961 aaaggataaa ttaacggggc acgaacttgg aaaactacta ataaaggatt gtattactgg
     1021 gatcatatca gcatataatg acaaggatat tatggcaggt acaaaaccag aggagcatat
     1081 attagataca tttgaaatta ataatcttat agatgggctt acaggcactt acaatataca
     1141 aatttacaag gattatagag aaatatggaa gtttttatcc gacatttcag taatgcagat
     1201 gatttatgct tatgttatag agattgcctt ttggaaactg acttcagcaa tcagccatca
     1261 tgatacatgt cctttagaag atgatgaaat agacggtatg ataaaagcta ttaatgaaac
     1321 aaatgaaaac ataaagaagt ttttcttgtt agaagcaata aaggaactaa ttgcaagata
     1381 tactcaggta tatgaaattt tacagacaat gacggctttt cctgaagatc tttttctata
     1441 tgccaatatg ttaattgata aatataatgt tctttataag caggcagata ttctgccaat
     1501 agtaaaaatg tgtcctatca cagaagaaaa aattcaggaa gttttaggga aaataaatga
     1561 agtatctacc ctatataaca tgaaaaaact ggaagattta ttattatcag agaacaaaga
     1621 aacctgtatt gtaggaggaa aagaagatga agaatattga tttaatgagt atgacaaaag
     1681 ggaaactttc cggtgaagag atggggaagc ttataatgaa acactatttc actgatttac
     1741 tggaaggtga aaagaataat cctatcccgt ttcctgagtt aaaaagaata gtaaatactc
     1801 ttcaggggag ccgaaatatt gagatatata aacagtatga aaagttgtta cagtttttag
     1861 tccaggaaag tatccgtatg gatgactata gattaaaaat ggagcttatg ttatggaaag
     1921 tctctttttt attgacctat agccttacaa gtaaatccaa accttttgag cctaaagaaa
     1981 tagaaggttt aaccataatg atatatagca taaatgatat gataaaacag tttttctttc
     2041 ttcaagttat catggaactg ataataaaga taacaaagat agaagaaatt tactgctttt
     2101 ttagcacctt tccggtgaga ttaataaaag ccacaaataa actgattgaa attacagaag
     2161 aaagccttcc tttgatagag tataatgtga tgactcccga aacttttaaa aaagcactgg
     2221 caggagtagg agacatttca gacctgcagg atgaagagaa aatgaggggg cttttaggaa
     2281 taccggaaag tttaaatata ccagttttgt cagagggccg gactgctata agctatgaag
     2341 aactggcaaa attataagta ataaaaagtt ctcaggttgt atttccagat ctggagatgg
     2401 gcctgagaac tttttattta ctggctttcc atgccagtaa gattaattcc ctttcctatg
     2461 gagaaattat tttcagagat tgcttatagt gactgacttc cacagagggg agaactccaa
     2521 aaaacctcaa aatttttcta ttttccgaat tttcggagac cataaaaaaa ggaaagccga
     2581 taaagcataa agaccgacaa aacccctggc atgtgcaaaa ttacctgact ggacaggtga
     2641 ggtataattg agatgggggg aatgttttat ggttgaaaaa gaaataacaa tccctaaaga
     2701 agtatatgaa caattacctt gggcactcga attatgttta acagatcctt taattgaaaa
     2761 agataaagag tctcttaatg gtttttacgg gatgtatcaa aaaatagaat attatcacgg
     2821 agataaatat aaacatcttt tagataaaat aaactctctt aaatatggca aggaagctat
     2881 tgaagaagcc acggaagaac aattaagaag cacatttgag gtttatataa gtagaagtaa
     2941 a
//
LOCUS       DAOQWT010000164         2939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_36102_length_2939_cov_4.524619, whole genome shotgun sequence.
ACCESSION   DAOQWT010000164 DAOQWT010000000
VERSION     DAOQWT010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36102_length_2939_cov_4.524619"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKJ95_04035"
     CDS             complement(<1..325)
                     /locus_tag="PKJ95_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65450.1"
                     /translation="MIKEKVPDCRKSRTTLTQINYNTKYNPDEDIEEKPEEAPEDMSV
                     RDFEPCRKTPVETCPDCSGQLEETTICGLDPQDTIDIFVCSRCSWKERRFIDDMTANE
                     VLDLLE"
     gene            complement(442..530)
                     /locus_tag="PKJ95_04040"
     tRNA            complement(442..530)
                     /locus_tag="PKJ95_04040"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(620..1339)
                     /locus_tag="PKJ95_04045"
     CDS             complement(620..1339)
                     /locus_tag="PKJ95_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65451.1"
                     /translation="MKLNDFYADLKYSENKTEDQLINEFYYKVFPNLKEIKTITDITL
                     QKKGIDKILTLKNGKEIAIDEKRRRVDYGDILLEEWSNFEKKKKGWTGDPEKVTDYIV
                     YIIQSKIYIFPYGLLQLAWRKNYYQWREEYGIKEAQNPGYVTTNVPVPTDILFEAIKK
                     EMLQDINEPATEEPVLKEDPSITLLIKLSSKLETMSEKIDYLFNKDKKQSKRKNRADN
                     TINMFPEEEKIINLEDRKKSA"
     gene            complement(1326..2552)
                     /locus_tag="PKJ95_04050"
     CDS             complement(1326..2552)
                     /locus_tag="PKJ95_04050"
                     /inference="COORDINATES: protein motif:HMM:NF016921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HNR65452.1"
                     /translation="MNTLHIDPEFKSLIPPLSTEEKELLEKSILSEGCRDPLVVWQNG
                     SLTLIDGHNRYEICQRNGIKYDTKQLKFSNKEEVKDWIITNQLGRRNLTDSLREYFRG
                     KLYESRKKQEKGGDRKGQEYKNQCSQNVHIDPTPKRTIEAIAKEQKVSPKTVQRSYQY
                     SQAIDTIASVMGEEVKQKILNEEIKVTKEEVKTLANKKPEEQKKIVEKFQTGEVKTVN
                     QAILAVNRDKPREIKPLPIELYDVIYSDPPWRYEHVKNSNDSIEAHYPTMDLEEIKAM
                     KVPAAENTVLLLWVTSPKLEEGLQVLNAWGFNYRSSMIWDKSGIESGKNWIGMGYWFR
                     GQHELLLVGVKGKFSPPKEEFRFPSIYREFKTEHSKKPEFIYEMIEKMFPGHKYLELF
                     ARNTRENWRSWGNEVK"
     gene            complement(2549..2761)
                     /locus_tag="PKJ95_04055"
     CDS             complement(2549..2761)
                     /locus_tag="PKJ95_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR65453.1"
                     /translation="MSKGYRVKEERELQKITQSELSIRTGLTQKFISELENDKYSSIS
                     DQTMEILAQALNKTVPELFYNEEKVA"
BASE COUNT          741 a          594 c          446 g         1158 t
ORIGIN      
        1 tctctaataa atcaagaact tcatttgcag tcatgtcgtc gataaatctt ctttctttcc
       61 aactgcacct ggaacaaaca aaaatgtcga ttgtatcttg cggatcgagt ccgcaaatgg
      121 ttgtttcttc taattgtcca gagcagtcag gacaggtctc aactggagtt tttctgcaag
      181 gttcgaaatc tctcacggac atatcttcag gagcttcttc aggtttttct tcaatgtctt
      241 catctgggtt gtatttcgtg ttataattta tttgtgttaa tgtagttctg gacttacgac
      301 agtccgggac tttttctttt atcatcgacc ttacctcctt taaaaattat ctgcccggtt
      361 tcccggtcag gttcgccggg cagaacttga atctttacgc ttacactgtg ttccggttac
      421 tttttagaga accggaaaga ctggtcggaa gagaaggaat tgaaccttct tctatggttt
      481 tacagttttt attgtcgact cccaccgcat taccgttatg ctatcctccg aaaaattcac
      541 tccctctgga gtgacttaat taaatgaaaa aatttaaccg caattcctcc cgcaaccgtc
      601 ccggcgtccc aggaggcttc tatgctgatt tcttcctatc ttcaagattt ataatctttt
      661 cttcttctgg aaacatattt atagtattgt ctgctctatt ttttcttttt gactgttttt
      721 tgtccttatt gaagagatag tctattttct ctgacattgt ttctaattta gaagagagtt
      781 ttatgagtaa agttatcgaa ggatcttctt ttaatacagg ttcttccgtg gctggctcat
      841 taatatcctg caacatttct ttttttatag cctcgaataa tatatccgtc ggtaccggca
      901 cgtttgttgt aacgtatcca ggattttgag cttccttaat tccatattct tcacgccatt
      961 gataataatt ctttctccat gccagttgta acaagccgta aggaaagata tatatttttg
     1021 attggataat ataaacgata taatcggtta ctttttcagg atctcccgtc cagccctttt
     1081 tctttttctc aaagtttgac cattcttcaa gaagaatatc tccataatct acccttcttc
     1141 ttttttcgtc tatagcaatc tctttaccat tctttaaagt taaaatttta tctatacctt
     1201 ttttctgaag agttatatct gttatagttt ttatttcctt aaggtttgga aatactttat
     1261 aatagaactc attaattaat tgatcttctg tcttattttc agaatatttt aaatcggcat
     1321 aaaaatcatt taacttcatt accccagctc ctccagttct ctcttgtatt tctggcaaat
     1381 aattcaaggt atttatgacc gggaaacatt ttttcaatca tttcatagat aaactcaggc
     1441 tttttgctat gttctgtttt gaattctcta taaatagaag gaaatctaaa ttcttccttt
     1501 ggtggagaaa actttccctt tactcctacg agcaataatt catgttgccc cctgaaccag
     1561 taacccatgc ctatccagtt cttaccgctc tctattccgc ttttatccca gatcatggag
     1621 cttctataat taaaacccca tgcatttaac acctgcaacc cttcttcgag tttaggggaa
     1681 gttacccaga gtaataaaac tgtattttca gcagcaggaa ctttcatggc tttaatttct
     1741 tctaaatcca tagtaggata atgggcttct atggagtcat ttgagttttt tacatgttcg
     1801 tatctccacg gtggatcgct ataaatcacg tcgtaaagtt caataggtaa aggtttaatt
     1861 tctcttggtt tatctctgtt tactgcgaga atagcctgat ttacagtctt tacttccccc
     1921 gtctggaact tttctactat ctttttttgt tcttcgggtt tcttatttgc aagagtcttt
     1981 acttcttctt ttgttacttt tatttcctcg ttgagaattt tctgttttac ttcttccccc
     2041 attacagagg caatagtatc aatagcctgt gaatattgat aagatctttg aactgtttta
     2101 gggcttactt tttgttcttt tgctatagct tctattgttc tttttggagt aggatcaatg
     2161 tggacatttt gactacattg atttttatat tcctgccctt tcctatctcc gcccttttcc
     2221 tgcttcttcc tactctcata cagcttaccc ctgaaatatt ccctgagaga gtcagttaaa
     2281 tttcttcttc caagctgatt tgttattatc cagtccttca cttcttcttt attgctgaat
     2341 tttaactgtt tcgtatcgta tttaatcccg ttcctctggc atatttcata tctattatgc
     2401 ccgtcaataa gagtaaggct tccattctgc catacaacca gagggtcacg gcaaccttca
     2461 gaaagaatac ttttttcgag aagttctttc tcttctgtag agagaggagg gattaagctt
     2521 ttaaattctg gatctatatg aagggtattc atgcaacttt ctcctcattg tagaataact
     2581 ctggaaccgt tttatttaat gcctgtgcta atatttccat tgtttgatcg gatatagaag
     2641 aatatttgtc gttttctaat tcagagataa atttttgagt taatccagtt ctgatagaaa
     2701 gttctgactg tgttatcttc tgcagttccc tttcttcctt aaccctatat cctttgctca
     2761 ttttcttact ccttatcgta aacgtaatat ttattaccta tatacattat aattacttac
     2821 acgtaataag tcaatattaa aaacgtaaat tgtttaaaat tattccttat acgtaattgt
     2881 aatgttattt acactaatgt aatcatgtaa tcacttttga tgttatgtaa tcactttat
//
LOCUS       DAOQWT010000165         2937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_36147_length_2937_cov_3.185288, whole genome shotgun sequence.
ACCESSION   DAOQWT010000165 DAOQWT010000000
VERSION     DAOQWT010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36147_length_2937_cov_3.185288"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKJ95_04060"
     CDS             complement(<1..249)
                     /locus_tag="PKJ95_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNR65454.1"
                     /translation="MDMKFLMKQAQEMQKKMEVIKEGLAHKEIKVTSGGGMIELIING
                     QQEIKEIHIEPDIIDPEDKEMLEDLVLAAVNEGIRQSKE"
     gene            complement(268..1983)
                     /gene="dnaX"
                     /locus_tag="PKJ95_04065"
     CDS             complement(268..1983)
                     /gene="dnaX"
                     /locus_tag="PKJ95_04065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNR65455.1"
                     /translation="MTQYQSIYRKWRPQSFEDIVGQNHITQTLKNAIKMNRIAHAYLF
                     SGPRGVGKTTTARIFAKALNCEKGPTETPCGQCSRCIRISQGQSLDVLEIDGASNRGI
                     EEIRELRSKIGFAPAEGKYKIYIIDEVHMLTDPAFNALLKTLEEPPSQVLFIFATTAP
                     YKVPNTILSRCQSFNFRRISIEEMVEKLKKITDEEKINIDSSSLRLIAESATGSMRDA
                     ESILDQVIAYSENKVTAQEVRDILGLLPHEIFYQFFDNIINHNTETALKLINKLVKEG
                     VELNKFVQDLIIYAHNASLLKVLGKDNPYSSLYFEADELDSVRGLIEKTEARTLLDII
                     EELKTVEEKMRFNQYPWVLLELTVVKLTHNEKKTIPQEALLESKSTISKKDIFTKDEA
                     IKKVEKRPTLLEKKDQDEGIPEEKKAVQRDIGRGFEFEKLWPKVLSRIKKEKISLYAF
                     LKAGNCVRIEDDQLIINFKTEYLFHKESLEKKENRKKIEEILKEETNCDLILKCILEE
                     SIAGESEVSDITENENITEEESTVGKLAQKTKKSNDGNKKEELTLEDILNEARDLFGG
                     EISEE"
     gene            complement(2031..2294)
                     /gene="ffs"
                     /locus_tag="PKJ95_04070"
     ncRNA           complement(2031..2294)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKJ95_04070"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT          794 a          632 c          440 g         1071 t
ORIGIN      
        1 ttcttttgat tgcctgattc cctcattcac tgcagccaat accaggtctt ctaacatttc
       61 tttatcttca gggtcaataa tgtctggttc aatatgaatc tctttaattt cctgctgccc
      121 gtttattatc aactctatca taccacctcc ggaagtaacc tttatttctt tgtgggcaag
      181 cccttctttg ataacttcca tctttttctg catctcctga gcctgtttca tcaaaaattt
      241 catatccata ttaaataatt tctcctctta ttcttcactg atttctccgc caaataggtc
      301 acgagcttca ttcaatatat cctccagagt taattcctct tttttattac catcgttact
      361 tttcttcgtt ttttgtgcca gtttaccgac agtgctctcc tcttcagtta tattttcatt
      421 ttcagtaata tcggagactt cactttctcc agcaatgctc tcctcaagaa tacacttcaa
      481 tattaggtca caatttgttt cttcttttaa aatttcctct atcttttttc tattttcttt
      541 cttttccaaa ctctctttat ggaaaaggta ttctgttttg aaattgataa ttaattggtc
      601 atcttcaatg cgtacacaat ttcccgcttt taaaaaggcg tagagagaaa ttttttcttt
      661 ttttattctg gatagtacct ttggccataa cttttcaaac tcaaagcctc tgccaatatc
      721 cctttgtact gccttttttt cctcaggaat accttcatcc tgatccttct tctctaacag
      781 ggttggtctc ttttctacct ttttaatagc ttcatcttta gtaaaaatat cttttttact
      841 gatagtactt ttactttcca gaagggcttc ctgtggaata gtcttctttt cattatgagt
      901 cagtttgact accgttaatt ccaataagac ccaggggtac tgattaaatc tcattttctc
      961 ttcaacagtt tttaactctt caataatatc aagcaaggtc cttgcctctg tcttctcaat
     1021 cagcccccgg acactgtcca gttcatcagc ctcaaaataa agagaggaat aaggattatc
     1081 tttacccaaa accttaagga gagaggcatt gtgagcatag ataatcaaat cctgaacaaa
     1141 tttattcaac tccactccct ctttgaccag cttatttatc aatttaagcg ctgtttcagt
     1201 attatggtta ataatattat caaaaaactg gtaaaatatt tcatggggaa ggagtcccag
     1261 aatatccctg acttcctggg ctgttacctt attttcacta taggctataa cctgatctaa
     1321 aatactttct gcatctctca tagaaccggt agcgctctct gctattaacc gtagagagga
     1381 cgagtcaatg tttatttttt cttcatcagt aatttttttt aatttttcca ccatttcttc
     1441 aatggaaatt ctacgaaaat taaaagattg gcagcgtgag agaatagtat tgggaacctt
     1501 atatggtgca gtagtggcaa aaataaataa aacctgactg ggtggttctt ctaaagtctt
     1561 gagtaaagca ttaaaggcgg gatcggtcag catatgtact tcgtcgatga tatatatttt
     1621 atacttgcct tccgccggtg caaatccaat tttacttctt aactctctga tttcttcaat
     1681 tccccggttt gaagccccat ctatttctaa cacatctaaa gactgcccct ggctgatacg
     1741 aatacatcgg gagcactgcc cacagggtgt ttccgtgggt cccttttcac agttaagcgc
     1801 cttggcaaaa attcgggcag tagttgtttt tcccactcca cggggaccgg agaaaagata
     1861 ggcatgggca atgcgattca tcttgatagc attttttagg gtttgtgtga tgtgattctg
     1921 gcctacaata tcttcaaaag actgaggtct ccattttcta tagatagatt gatattgagt
     1981 catatatggt ttccctcaca atagtgatat actccttatt aaaaagacga ccgtgcccta
     2041 ccctcgaacc accttcccaa gcgataatga aacagttaac tccaatcagg cttccctgcg
     2101 gcacccaaag agatttactt accgctgctt ccttccagac ctgacggggt ttgtaattct
     2161 ttgctgcaca ggacccaatc ttcaacatca cttattccac ccggacctta aaaagataag
     2221 ccctcaaata ggttttcgat cctgctctag cggattgcag gttcagggca ccgctaactc
     2281 cccacctagc acggtcatca cttaaaagca tatcataaag gggaatcctt cccctttaga
     2341 acccctgaaa gactttcttc cacttaagga tgtaatttgt atatattttg ttgtatttag
     2401 actgaagttc ataatctcag gtgatatatg tcttcagcct ataaagggga atccttcccc
     2461 tttaaaaccc ctgaaataca gttcttaact aataatgcag ttgatcatta ttttttattt
     2521 taattatcct gaggttcata accgcaggtt ataactatcc tcaggatcat aaaggggaat
     2581 ccttcccctt gttattctaa aatacaatac taagttaata atgcaaatag tttattatat
     2641 tttgttttta cttatcctga actgaaaagt tataatttta ggtaagcttc ttacctcaga
     2701 ataacagttt ttgatcagtt tagtgataaa ttatttataa tataatggat tctctaatct
     2761 ctaataattt aattttataa ttattagttt ttattctctg taaatattat ctgggttgat
     2821 ttttagaaaa tgtattggaa atattattat tacttttcat aattgctatt ccttttcaat
     2881 cgctttcagc actttgacgt ataaattctt cacaccgctg tttttctctt tcttgat
//
LOCUS       DAOQWT010000166         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_36615_length_2913_cov_3.172498, whole genome shotgun sequence.
ACCESSION   DAOQWT010000166 DAOQWT010000000
VERSION     DAOQWT010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36615_length_2913_cov_3.172498"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKJ95_04075"
     CDS             complement(<1..500)
                     /locus_tag="PKJ95_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNR65456.1"
                     /translation="MQDNNDLTIPKIEQISNSVQLLYQKAKNGEIEVSKIFLLKILED
                     YLTFLLTTHSESINLEIVANFLMSVSELLLWKTNLLLPFSQTQIVEEIESVADNFKEN
                     YWKEYKKYQSLVPILLKKEYKQREIYLTCLSSIDDFEEIPQNYDFSELILALESVLAK
                     DNSANV"
     gene            complement(490..1479)
                     /gene="trpS"
                     /locus_tag="PKJ95_04080"
     CDS             complement(490..1479)
                     /gene="trpS"
                     /locus_tag="PKJ95_04080"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNR65457.1"
                     /translation="MKGVIFSGMRPTGKLHLGNYFGALTNWIKLQDEYQCYYGVVDWH
                     ALTTGYDSTNMMKENTIEMVIDWLSAGIDPNKSTLLIQSYIPELAELHLLLSMITPLS
                     WLERNPVVKEQVRDLDLKENMGYGLLGYPVLMAADILIYKTNTVPVGEDQAPHIELAR
                     EIGRRFNHLYRDVFIIPEIKLSPTPKILGTDGRKMSKSLNNYISLSDQPEVIKNKVRS
                     MITDPEKIKLSDPGHPEICTVFDYHRMFNIIELADIENRCRNAQLGCVACKERLSDIL
                     INFFQEFRERRKCYEKNKYQVWDILERGSQKAREVAKITLHEVKKAMKMDYAG"
     gene            complement(1527..2162)
                     /locus_tag="PKJ95_04085"
     CDS             complement(1527..2162)
                     /locus_tag="PKJ95_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNR65458.1"
                     /translation="MSDQIFEIIWTIPVILIAITTHEYAHGLVANYYGDPTAKLAGRL
                     TLNPIAHLDPAGIIMFLIFRIGWAKPVPVNSQNLKNPKMDMIKVSFAGPFSNIILAFL
                     FSLLLKVNYFIFQKVIFNSYFWFRVAQGWFTLLRSGIFINIALAIFNLIPIPPLDGHH
                     IVSGILPEHLARQYEKINQTYGMLIILFLFISGIVRKIILPIVDYLYKIFI"
     gene            complement(2167..>2913)
                     /locus_tag="PKJ95_04090"
     CDS             complement(2167..>2913)
                     /locus_tag="PKJ95_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65459.1"
                     /translation="EKRYNFTIENSTIKLLKKAVKEGLVSRIRKKRLSEEFLIILQEK
                     DPVKVLKRMDELEILAGIIPNLSLPSGLIERFVEIEDFTKYWHQKFFHEGFNKSILYL
                     FYLLQFSQLEIKILDKKIELSKKIATTLVEIRDCKEETINFLEQEFVAPSNIYYYLHN
                     KSNELLFVILLEKYKDSLIRERIKKYLTIYKKTTTFLSGKDLTDLGMKPGPIYAKILK
                     LVFYLQMNGILNSREEEIAFLTRLLAKGII"
BASE COUNT          920 a          494 c          422 g         1077 t
ORIGIN      
        1 attacattgg cgctattatc ctttgcgaga acagattcca aagctaagat tagttcagaa
       61 aaatcatagt tttggggaat ctcttcaaag tcatctattg agctgaggca ggttaaatat
      121 atttctcttt gcttatattc ttttttaagg agtataggta ccagtgattg gtatttcttg
      181 tattctttcc aataattttc tttaaaattg tcagcaacgc tttctatttc ttcaacaatt
      241 tgggtttggg aaaatggtaa taatagattt gttttccata atagtaattc tgatacactc
      301 attaaaaaat ttgccactat ttctaaattt atagattctg aatgagttgt aagaagaaat
      361 gttaaataat cttccaggat ttttaataaa aatattttgc tgacttctat ttccccattc
      421 ttcgcctttt gatacaagag ctgaacagag tttgatatct gctctatttt aggtattgtt
      481 aaatcattgt tatcctgcat aatccatttt catagccttt ttaacttcat gtaaagttat
      541 ctttgctact tcccttgcct tctgactacc tctctccaat atatcccata cctggtattt
      601 atttttttca taacactttc ttctttctct aaattcttga aaaaaattta tcaatatatc
      661 agataaacgc tccttgcagg caacacagcc taattgggca ttccgacatc tattttcaat
      721 gtctgccagc tcaataatgt taaacattct gtgataatca aatacagtgc aaatttcggg
      781 atgcccaggg tcagataatt ttattttctc gggatcagtt atcatagacc taaccttatt
      841 tttaataact tctggttgat cagataaact aatataatta tttaagcttt tactcatttt
      901 tcgaccatct gtacctaaaa ttttaggagt aggagatagt tttatttcgg gtataataaa
      961 tacatctcgg tataaatggt taaatcttct accaatttcc cgggctaatt ctatatgggg
     1021 ggcctgatct tcaccaacag ggactgtatt cgttttataa atcagtatat ctgccgccat
     1081 cagaacggga tagccaagca atccatatcc catattttct tttaaatcca ggtctctcac
     1141 ctgctctttc actactggat ttctttccag ccaggaaagg ggtgtaatca ttgataataa
     1201 taaatgtagc tcagctagtt caggaatgta agattgtatc aataaagtac ttttattcgg
     1261 atcaattcca gcacttaacc aatcaattac catttctatt gtattttctt tcatcatatt
     1321 tgtactatca tacccggtag tgagtgcatg ccaatctaca acaccataat agcactggta
     1381 ttcatcttgg agttttatcc agttggtcaa agcaccgaaa taatttccta aatgcaattt
     1441 gccagtcggc ctcataccgc tgaaaataac acctttcatg ttagttgccc cctccttgag
     1501 gtttattaaa aaatagtatc attattttaa ataaatattt tataaagata atccacaatg
     1561 ggtaaaatga tttttcttac gataccggaa atgaataaga aaagtattat aagcattcca
     1621 taagtttgat taattttttc atattgcctg gctaaatgtt ctggtaagat acctgaaaca
     1681 atgtggtgtc catccaatgg agggattggt attaaattaa aaatcgccag agcaatattg
     1741 ataaaaattc cagaacgcag taaagtaaac catccttgtg ctactctaaa ccaaaaataa
     1801 gaattaaaaa tcactttttg aaaaatgaaa taatttactt ttaataacag agagaataaa
     1861 aatgcaagta taatattaga aaaaggtcca gcaaaggata ctttaatcat gtccatcttg
     1921 ggatttttta aattttggct gttaacagga acaggttttg cccaaccaat tcgaaatatt
     1981 aagaacatga taatccctgc tgggtcaaga tgtgcaattg gatttaaggt taatcggcca
     2041 gctagttttg ctgttggatc tccgtaataa ttagcaacta acccatgagc atattcatgt
     2101 gttgttatgg caattaatat tacgggtatg gtccatataa tttcaaaaat ttgatctgac
     2161 ataaaattaa ataattcctt ttgctaataa tcttgttaag aatgctattt cttcttcccg
     2221 gctattcaat ataccattca tttgtaaata gaatactaat tttaatattt tagcataaat
     2281 agggccaggc ttcattccta aatcggtcaa atctttccca gaaaggaaag tagttgtctt
     2341 tttatatatt gtcaggtatt ttttaattct ttctctgatt agactgtctt tgtatttttc
     2401 taataatatt acaaataaga gttcgtttga tttattatgc aggtagtaat atatattact
     2461 aggggctaca aactcttgtt ctaaaaaatt tatggtctct tctttacaat ctcttatttc
     2521 tactaaagta gttgcaattt ttttactcag ttcaattttt ttatctaaaa tcttaatttc
     2581 tagttgagaa aattgtaaca aataaaaaag atagaggata cttttgttaa aaccctcatg
     2641 gaaaaatttt tgatgccaat atttagtgaa atcctcaatt tcaacaaacc tttcaattaa
     2701 acctgatggg agggaaagat tcggtattat tcctgcaaga atttccaatt catccattct
     2761 ttttagaact tttaccggat ctttttcctg tagaataatc aagaattcct cactgagtct
     2821 tttttttctt attcgagaaa ctaacccttc tttgaccgct ttctttaata atttaatagt
     2881 tgagttttct atggtaaaat tatatctctt ttc
//
LOCUS       DAOQWT010000167         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_36776_length_2904_cov_3.095121, whole genome shotgun sequence.
ACCESSION   DAOQWT010000167 DAOQWT010000000
VERSION     DAOQWT010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36776_length_2904_cov_3.095121"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PKJ95_04095"
     CDS             <1..449
                     /locus_tag="PKJ95_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNR65460.1"
                     /translation="KSFIEEHFPNLVAGKDFDFFPFPPIKKEFGNPALGAADMLSMFN
                     DTPEARAFMKYIASAPAQEIWVGELGKLAPNQKVNPSVYPDDITRKAAQFLGEASVFR
                     FDGSDLMPAAVGSGSFWTGIMDYVSGESLQNVLLQIETSALDAYRQ"
     gene            633..1502
                     /locus_tag="PKJ95_04100"
     CDS             633..1502
                     /locus_tag="PKJ95_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNR65461.1"
                     /translation="MRTKWFTPWFYVIPALATLVFFLIYPSINTLIISFFGPQSKEFV
                     GFSNYIYCFTNETMLSAFRNNLLWVAIFVPFTVFLGLILAVMLDRVHYESLIKSIIFM
                     PMAISFVGAGVIWKFVYSYRPIGSGQIGILNAFLSYIGVQPIPWLIERPWLNNICLII
                     VGIWIWTGFCLVVLSASYKGIPRELLEAARVDGANEYRIFWKIILPLMKPTVAVIATT
                     MLINVLKVFDIVYVMTNGGFSTEVIANRMYKEMFLFRNYGRASAIAILLLLFIIPMIV
                     INVQRFKEQEATR"
     gene            1499..2359
                     /locus_tag="PKJ95_04105"
     CDS             1499..2359
                     /locus_tag="PKJ95_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNR65462.1"
                     /translation="MKPKFSQFLLNTPMHLFIILIAIIWVFPTVGLLITSFRPSSAVA
                     SSGWWTVLMHPFNFSQYTLANYYEVITKIGLGRTFLNTLIITLPACILPIIIASFAAY
                     AFAWMEFWGRRFFFILFVGLLVVPLQMTLIPILRIFNKLGLSGSFFGIWLAHAGYGLP
                     LVIYLMYNYISGLPSEIFESSTIDGATPFQIFKSIVIPLSVPAIASIAIFQFIWVWND
                     LLVALVYLGGTPNVAPLTVRISALVGSYGQNWELLTAAAFVSMVLPLIIFLSLQRYFV
                     RGILAGSVKG"
     gene            2536..>2904
                     /locus_tag="PKJ95_04110"
     CDS             2536..>2904
                     /locus_tag="PKJ95_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNR65463.1"
                     /translation="MSNLENNLKIIDKLAIGVDLGGSKIGIALVKKNGEIEKYVKIPT
                     NAEQGKELIINRIKQGIYQVIEEKDLQINNILGIGIGAPGPVDYKKGIIHCAPNLPEW
                     TDVPLSNIIKDEFKIPVIVEN"
BASE COUNT          846 a          495 c          565 g          998 t
ORIGIN      
        1 ttaagagctt tatagaagaa cattttccaa atttagtagc aggtaaagac tttgatttct
       61 tccccttccc gcccattaaa aaagaatttg gcaatcccgc cttgggtgca gcagatatgc
      121 tttctatgtt taacgacaca ccagaagcca gagcttttat gaaatatata gcatccgccc
      181 ctgcacagga aatctgggtt ggtgaattgg gaaaactggc acctaaccag aaagtgaacc
      241 ccagtgtcta tcctgatgat atcactcgga aagctgccca gtttttggga gaagcatctg
      301 tatttcgttt tgatggttct gacctgatgc ccgccgcagt aggttctggt tcattctgga
      361 ccggtataat ggattatgtc agtggagaat ctttacaaaa tgtattatta cagattgaaa
      421 caagtgcatt agatgcctat agacaatagt tgtaagataa aacttttacc aataaaatga
      481 aatgaatagt aatttaatag ttagctggtt tgttttatat aaaccagcta actattaatt
      541 tgtttcctct taaaagccac tttcttataa agtggaatga ttaaacagga gaaagtgcac
      601 ttaaaaaagt ttagataagg agatggacta tcatgcgaac gaaatggttt accccctggt
      661 tttatgtcat tcctgcttta gcaactttgg tctttttttt aatctatcct tcaattaata
      721 cacttattat ttcttttttt ggtccacaat caaaagagtt tgttggtttt tctaattaca
      781 tttattgttt taccaatgaa acgatgctat cagcattccg aaataatcta ctatgggtag
      841 ctatctttgt cccttttaca gtttttttag gattgatact ggcggtgatg ctggatagag
      901 tccattacga atcattgata aaatctataa tatttatgcc tatggctatc tcatttgtgg
      961 gggcaggggt tatctggaaa tttgtatatt cctacagacc gataggaagc ggacaaattg
     1021 gtattctcaa tgctttttta tcttatatag gagttcagcc cataccctgg ttaattgagc
     1081 gaccctggtt gaataatatc tgtttaataa tagttgggat ttggatctgg accggttttt
     1141 gtttggtggt tctttctgcc agttacaaag gaattcccag agaattattg gaagcagcca
     1201 gagtagacgg tgccaatgag tacaggattt tctggaagat tattctgcct ttaatgaaac
     1261 caactgtggc agttatagct accaccatgc tcataaatgt tttgaaggta tttgatattg
     1321 tctatgttat gaccaatggt ggattttcca ctgaggtcat tgcaaacaga atgtataaag
     1381 agatgtttct attccgaaac tatggaagag ctagtgcaat agcaatcctg ttactcctct
     1441 ttataattcc tatgattgtt attaatgtgc agcgtttcaa ggaacaggag gctacacgat
     1501 gaaaccaaaa ttcagccaat tccttttaaa tacacctatg catttattta ttattctgat
     1561 agcaattatc tgggttttcc ccactgttgg gctgttgatt acctcctttc gtccctcctc
     1621 agcagtagct tcttcaggat ggtggacagt cttaatgcat ccatttaatt tttcacaata
     1681 tacccttgcc aattattatg aggttattac caaaattggg ctgggtagaa ctttcctgaa
     1741 tacccttatc ataactctgc ctgcctgtat tttacccata atcattgcct cttttgcagc
     1801 atatgctttt gcctggatgg aattctgggg aagacgattt ttctttatac tttttgtagg
     1861 actactggtg gtacctttgc agatgaccct gattcccatt ttaaggatat ttaacaaact
     1921 ggggctttcc ggttcatttt ttggaatctg gcttgctcat gcaggatatg ggcttccctt
     1981 agttatttat ctgatgtaca actatatatc cggcttaccc agtgaaatat ttgaatcttc
     2041 tacaattgat ggcgctaccc ctttccagat atttaaaagc attgtcatcc ctttatcagt
     2101 gccggcgata gcctctatcg ctatctttca gttcatctgg gtatggaatg acctgttagt
     2161 tgcactggtt tatttaggag gaactcccaa tgttgctccg ttaacagtca gaatcagtgc
     2221 tctggtaggt tcctatggac aaaattggga gttgttaacc gctgctgctt ttgtctctat
     2281 ggtattacca ctaataatat ttttaagttt acaacgttat tttgtgagag ggattttggc
     2341 tggttctgta aaaggataat aaaaaggttt atagtttatg gtttttagtt tttagtataa
     2401 aatataagaa atattattat ttttttcttg cgaatatata tctaagtacc attttctcat
     2461 tacccatcac tcatcacaca caacggattt atggtatact ggtttactta actaacgaag
     2521 gagaattaag gggaagtgag taatttagaa aacaatttaa aaataattga taaattagct
     2581 attggtgtag atctgggtgg ttccaagatt ggtattgctt tagtgaaaaa aaatggtgaa
     2641 attgaaaaat atgtaaaaat acccactaat gcggaacagg gtaaggaatt aatcattaac
     2701 agaataaaac agggtattta tcaggtaata gaagaaaagg atttacaaat taataacatt
     2761 ttagggattg gtataggggc tcccggccct gtagattata agaaaggtat cattcattgt
     2821 gctccgaatt taccggaatg gacagatgtg cccctgtcaa atattatcaa agatgaattt
     2881 aaaataccag taattgtgga aaat
//
LOCUS       DAOQWT010000168         2863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_37586_length_2863_cov_3.598291, whole genome shotgun sequence.
ACCESSION   DAOQWT010000168 DAOQWT010000000
VERSION     DAOQWT010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37586_length_2863_cov_3.598291"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..158)
                     /locus_tag="PKJ95_04115"
     tRNA            complement(84..158)
                     /locus_tag="PKJ95_04115"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Glu,seq:ctc)
     gene            complement(174..247)
                     /locus_tag="PKJ95_04120"
     tRNA            complement(174..247)
                     /locus_tag="PKJ95_04120"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(213..215),aa:Gln,seq:ctg)
     gene            complement(407..1924)
                     /gene="gltX"
                     /locus_tag="PKJ95_04125"
     CDS             complement(407..1924)
                     /gene="gltX"
                     /locus_tag="PKJ95_04125"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNR65464.1"
                     /translation="MGIENKNIRVRFAPSPTGYLHIGGARTALFNWLYARHYGGTFIL
                     RIEDTDRARSTPEAVNAILDGMKWLGLDWDEGPEKDGEYGPYFQTERLDLYKKFIDRL
                     LESGEAYYCFCSAEELAERRKKKLAEGRPSIYDRKCLNLSDNEKKQLIKEGKKPAIRL
                     KMPDRQIVVDDLIKGIMEFDSKLLSDFVIIKSDGIPTYNFAAVVDDILMKVSLVMRGD
                     DHISNTPKQIVIYQALGIPLPEFAHIPMIMGPDNTRLSKRHGATSVIEYQKMGFLPEA
                     VVNYIAHLGWSSGTNQEIFTIKELVESFALEKISHHSAIFNMEKLNWFNNEHLKRLSD
                     EKYVEMLIPFLIEARYIELPLSEKKEKWLRKVVSLMKSRARNFKQFLEYGDYFFTDDF
                     IIEEEAAKILEQTGVKDILEQLINKLKEIDKWDEENIECKVREMAHQLNLKGKQIIHP
                     ARVSLSGKTVGPSLFALMEVLGKERNIKRLEKIISKLSKNSEYTFPKPDNGKRVN"
     gene            complement(2000..>2863)
                     /locus_tag="PKJ95_04130"
     CDS             complement(2000..>2863)
                     /locus_tag="PKJ95_04130"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HNR65465.1"
                     /translation="LNLNSNEYNIGHKSVVDNKYKPKETLEQEQVQSVSEKRVLFLPE
                     YFEQKGKETPLLDRFLTDRKRSEIKAKVAIIIDDIGYHKDIAEQIMNLNFPVAISVLP
                     FLPHTHIVAEKAKEKGFTLLLHLPMEPHNSNTDPGKGAIFTTMNEQEIRAKILADLEE
                     IPYSDGVNNHMGSKATEDNYLMRIVLNELKERNLFFIDSMTSPNSVGYKLSKEMGIKT
                     AQRTVFLDNEQDIDYIRNQVTILKDSALRYGNAIAIGHPYCNTIEVLRELKSILTPAG
                     VVIVPLDELLE"
BASE COUNT          824 a          592 c          393 g         1054 t
ORIGIN      
        1 tcttatgtta aaaaaagcag gaatgacctg ctcttttatt ttttacttta atttttataa
       61 ctcagccaaa tctatttttt aattggtggc gcaaggggga attgaacccc cgtttgctga
      121 ttgagagcca gctgaattaa ccactattcg attgcgccat cttatatttt atatggctga
      181 gggaggtggg gtcgaaccac cgctcccaga tccagagtct ggtgtcctac cactagacga
      241 tccctcaaaa tttttatagc tacaacaata atattttaca acaaattttt tataggtcaa
      301 gccatttcaa taaaggacga aaatttcaac ttattgttac cggtaattat tgctacattt
      361 tatactttca agaggttttt gtttaaactc ttttacaatt attcttttaa tttactcttt
      421 taccattatc aggcttaggg aaagtatatt cagaattttt acttaattta ctaattattt
      481 tttctaatct tttgatattt ctctctttac ccagtacttc catcagggca aataagcttg
      541 gaccaacggt cttaccagag agcgagacac gagcaggatg aataatttgt tttcctttta
      601 aatttaactg atgtgccatt tctctcactt tacattcaat attctcctca tcccatttat
      661 ctatctcttt aagtttattt atcaactgct ctaatatgtc ttttacccct gtttgttcta
      721 atattttcgc cgcctcttcc tctatgataa aatcatcagt gaaaaaataa tccccatatt
      781 ccaaaaattg tttaaaattt cttgctctac ttttcataag tgaaacaact tttctaagcc
      841 atttctcttt tttttcactt aaaggcagtt caatatacct cgcttctatg agaaaaggta
      901 ttaacatttc tacatatttt tcatcagata atctttttaa atgttcatta ttaaaccagt
      961 ttaatttttc catgttgaaa attgcggaat gatgggatat tttttctaaa gcgaaacttt
     1021 ccactaattc ttttatagta aaaatttcct gattagttcc tgaagaccag cctaaatgtg
     1081 caatatagtt aacaactgcc tctggcaaaa aacccatttt ctgatattct ataacagaag
     1141 ttgcaccatg tcttttgctc aacctggtat tgtctggacc cataatcata ggaatatgag
     1201 caaattcggg aagagggatt cccaaagcct gatagataac aatctgcttt ggtgtattag
     1261 aaatatggtc atccccgcgc ataactaatg acaccttcat taaaatatca tcaaccactg
     1321 ctgcaaaatt ataggttggt attccgtcag attttattat tacaaaatca ctgagcaatt
     1381 tactatcaaa ttccattatt cctttaatta aatcatctac tacaatttgc ctatcaggca
     1441 tttttagtct gattgccggt tttttaccct cttttattaa ttgtttcttt tcattatcac
     1501 ttaaattcaa acatttgcga tcgtaaatgg atggtcttcc ttctgccaac ttcttcttac
     1561 gtctctctgc aagttcctcc gcactgcaaa aacaataata agcctctcca ctttctaaca
     1621 atctatctat aaatttttta tataaatcca gcctctctgt ttgaaaatac ggcccatatt
     1681 ctccgtcctt ttctggccct tcatcccaat caaggccaag ccatttcatt ccatctaaaa
     1741 tagcattaac ggcttcagga gtagaccttg ccctatcagt atcttctatt cttaaaataa
     1801 atgttccacc ataatgcctg gcgtagagcc aattaaatag agctgttctg gcacctccaa
     1861 tgtggaggta tcctgtcgga cttggagcaa atcttactct aatatttttg ttctcaatcc
     1921 ccatatttcc cctccggctg tatttttctc tttttatata tctatatatc ttactgattt
     1981 aacattataa tattttactt tattccaata actcatctaa aggaacaatt acaacaccag
     2041 ctggtgttaa aattgatttt aactccctga gcacctcaat ggtattgcaa taaggatgtc
     2101 caattgctat ggcgttacca tatcttagag ctgaatcttt caaaatggta acctgatttc
     2161 ggatatagtc aatatcctgc tcattgtcta aaaagacagt gcgctgagcc gtcttaattc
     2221 ccatttcttt acttaatttg taacctactg aattaggact ggtcatacta tctataaaga
     2281 ataaatttct ctcttttaat tcattcagga caattctcat taaataattg tcttccgtgg
     2341 cttttgaacc catatggttg tttacaccat cactatatgg gatttcctcc agatctgcta
     2401 aaatctttgc cctaatttcc tgttcattca ttgtagtgaa gattgcacct ttaccagggt
     2461 cagtatttga attatgcggt tccataggca aatgtaacaa gagagtaaat cctttttctt
     2521 tagccttttc tgctactatg tgggtatgtg gtaaaaaggg tagcacagat atagcaaccg
     2581 ggaaatttaa attcataatc tgctccgcaa tatctttatg atatccaata tcatcaataa
     2641 taattgccac tttagctttt atctcgctcc tctttctatc tgttaaaaat ctatcaagca
     2701 agggtgtttc tttgcctttc tgctcaaaat attcaggtaa aaaaagtacc cgcttttcac
     2761 ttacagactg cacctgttct tgctcaagag tctccttggg tttgtattta ttgtcaacaa
     2821 ccgatttatg cccaatatta tattcattgc tgtttaaatt caa
//
LOCUS       DAOQWT010000169         2850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_37860_length_2850_cov_2.605725, whole genome shotgun sequence.
ACCESSION   DAOQWT010000169 DAOQWT010000000
VERSION     DAOQWT010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37860_length_2850_cov_2.605725"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1220
                     /locus_tag="PKJ95_04135"
     CDS             174..1220
                     /locus_tag="PKJ95_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNR65466.1"
                     /translation="MNITELLHLTSDMEASDLHLTVGVPPTLRIHGLLTKLDYPELNE
                     SDVYEMLYSILSEEHRKKFETLKEIDFSLEIVNGARFRVNVFMHRRGIAGAFRLIPEQ
                     LKTLEELGLPQNLSEFTKKPKGLVLMTGPTGSGKSTTISTLIDIINEKQRLHIITIED
                     PIEFIHHHKNCIIDQREVGLHTNSFSYALRSALREDPDVIMVGEMRDLETISMAVTAA
                     ETGHLVFSTLHTNSAAETVERIIDVFPAHQQRQIRIQLAESLQGVVTQTLLPNVDQTK
                     RLPAVEIMIATPAIKNIIREERIHMIPAIIQASQQYGMQTMDQSIRQLFLSHQIDRET
                     ALSKANDPKFVVSY"
     gene            2244..>2850
                     /locus_tag="PKJ95_04140"
     CDS             2244..>2850
                     /locus_tag="PKJ95_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNR65467.1"
                     /translation="MLAKVPKGTNDILPEQIDCWYYLENKTREVLQNYGYREIRTPAF
                     EHTELFVRGIGETTDIVQKEMFTFQDRKGRSLTLRPEGTAPVVRAYLEHNLGRENPLS
                     KLFYIGSMFRCEKPQAGRYRQFNQFGAEAIGSPLPVIDAEIIAAALSIYKQLGLTDLK
                     LMLNSVGCRKCRPQYLKALKDYFGEKREMLCVDCRQRYEQNP"
BASE COUNT          977 a          478 c          585 g          810 t
ORIGIN      
        1 aaaaagacag ctgcttatgg tcattttggc agggaattgg aagaatttac ctgggaaaaa
       61 acagataagg ctgacctgtt aaagaatgaa atataatgga gaactaaata taaaatattg
      121 gtatccttct tgtgtcccat ttcttattca aaaataaact atgaggtatg ataatgaata
      181 taacagaatt actgcatctt accagtgata tggaggcgtc tgacttacac ctgaccgttg
      241 gtgtcccgcc taccttgcga atccatggtt tacttaccaa attggattat cctgaattaa
      301 atgagagtga tgtttacgag atgctctata gcatattgag tgaagagcac cgtaaaaaat
      361 ttgaaacact aaaagaaatt gacttttctt tagagattgt caatggagct cgtttcaggg
      421 tgaatgtttt tatgcacagg cgtggtatag ccggtgcctt tcggttaatt cccgaacaac
      481 ttaaaacatt ggaagaatta ggactacctc agaatttatc cgaattcacc aaaaaaccaa
      541 aaggactggt tttaatgacc ggacctaccg gaagcgggaa atcaacgact atctctaccc
      601 ttattgatat tattaatgaa aaacaaaggc ttcatattat aaccattgag gaccccattg
      661 aattcattca tcaccacaaa aactgtatta ttgatcagcg tgaagttggg cttcatacta
      721 attccttttc ctatgcctta agaagcgcct taagagaaga ccccgatgtt ataatggtgg
      781 gggaaatgag agatttagaa actatatcta tggctgttac tgcagccgaa acaggtcatc
      841 tggtattttc taccttgcat accaatagtg ctgccgaaac agtggaaaga atcattgatg
      901 ttttcccggc acatcaacag aggcaaatcc ggatacagct ggctgagtca ctgcagggag
      961 ttgtaaccca gactttacta cccaatgtag accagacaaa acgattgcct gctgtggaga
     1021 taatgatagc aacgccggca attaaaaaca ttatcaggga agaacggatt catatgattc
     1081 cggctataat acaggcatca cagcaatatg ggatgcagac aatggatcaa tccatacgtc
     1141 aattgtttct atcacatcaa atagacaggg aaacagctct atccaaagcc aatgacccaa
     1201 aatttgtagt atcttattaa aagatgtctc ttaaaataaa tgtggttgtt taacaaaaaa
     1261 tcaaaaaaaa gtatatgtca aaaaatgttt taatctaaaa tgaactacac aatatttttg
     1321 actttaaagt cactattgag taaaatattt ccagaatttg tactttttga aaattaatat
     1381 taataaatat tggaaataga tggaaagggg agagtagatt gcttcttttc gaatcgaaag
     1441 acatccccgg gcatcatttt taaatgatag cttacaacat ctgtctattc gttacagtgc
     1501 gttaaaagaa tgagcggaat ggcttttcta accattcaaa aagggtggca ccgcgaaaat
     1561 aaattttcgt ccctgatagt attatttgta atttataata ttatcagggt ttttctttaa
     1621 ttattattgt gggtataatt gagagttata aagtttttag tttatagtta atagttttta
     1681 gtaaaagaca aagaaaatgc aaagatatga taaagggtgt gggtataagt gtatgcaaaa
     1741 atgtcgttgg ccgttggtgt ttagtcgttg gttaaaatgc aaagaaaaag atgcggggaa
     1801 aagtgaggga gtaaaaagat aaagaggact ttcctctcct gtcattgcga ttgagagcga
     1861 acgtagagag cgaagcaatc tcatccactc ctgcccgcaa tgaatgtagt aatgagtgat
     1921 agataaaaaa taataagcaa aatactaata gtctgcaacc agaaagaaaa attattatgt
     1981 ggaaataatt ggatgtgatt gccacgacat actttcagta tgtctcgcaa tgacaaaaaa
     2041 tagggatcat tgcgaagcgg tgcaaccact gctgaattct cgtactctga tgtcaaatat
     2101 tttttacatt tatattggtc tttatatcta actaaatact aaataccaac gaccatcaac
     2161 tatcgactga cgactatcga ccatcgacca aaatgtcatt acaaatgacg cattatgaag
     2221 ttatgaagat aaaggagaga attgtgttag caaaagtacc caagggaacc aatgatatac
     2281 tgcctgagca gatagattgc tggtattatt tggaaaataa gaccagagaa gtattacaga
     2341 attatggcta tcgggagata aggactcctg cctttgagca tactgaatta tttgtaagag
     2401 ggattgggga gacaaccgat attgtacaga aagagatgtt tacctttcaa gaccgaaaag
     2461 gcagaagcct taccctccga cctgagggaa ctgcgcccgt ggtaagagcg tatttggagc
     2521 acaatcttgg cagagaaaac cccctcagca aactgttcta tatcgggagt atgtttcgtt
     2581 gtgaaaagcc gcaggcaggc agataccgtc aatttaatca atttggtgca gaggctattg
     2641 gctctccttt acccgtaatt gatgcggaga taatagctgc tgccctctct atttataaac
     2701 aattaggatt gactgattta aaactgatgc tcaatagtgt aggatgtcga aaatgcaggc
     2761 ctcaatattt aaaagcatta aaagattatt tcggggaaaa gagggaaatg ttatgtgttg
     2821 attgccggca gcgttatgaa caaaatcctc
//
LOCUS       DAOQWT010000170         2846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_37932_length_2846_cov_2.843067, whole genome shotgun sequence.
ACCESSION   DAOQWT010000170 DAOQWT010000000
VERSION     DAOQWT010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37932_length_2846_cov_2.843067"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PKJ95_04145"
     CDS             <1..1191
                     /locus_tag="PKJ95_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNR65468.1"
                     /translation="SALEAISQEAPIIQLVNMIIVDAIKERASDIHLEPNQRGLLVRF
                     RIDGILHDVRMLPQRVRPAVVSRVKILSNMDIAERRLPQDGRFQLRFGAREVDLRVSS
                     IPTVYDEKIVMRILDKSQGVIALESIGFTEAQVQDFRKMIHYPHGIILITGPTGSGKS
                     TTLYATLNEVDSAKKNVITVEDPVEYKLDRINQINVRPKINLTFANALRSILRQDPDI
                     IMIGEMRDSDTAHIAVQSALTGHLVFSTLHTNDAVSSLTRMTDMGVEPFLISSSVIGV
                     MAQRLVRRICPKCIEEYIPEEGIFDDLKLNVVLPDKDKIRLFRGKGCDYCKNTGYYGR
                     IAIFEMIEINDEIRSMILKKISSNQIKEVAIRYGMKTLLDSGIEKALEGITTIDEVFR
                     VAVS"
     gene            1236..>2846
                     /gene="alaS"
                     /locus_tag="PKJ95_04150"
     CDS             1236..>2846
                     /gene="alaS"
                     /locus_tag="PKJ95_04150"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNR65469.1"
                     /translation="MNSNQIRDTFLQFFQSKGHKIMPSASLIPEDPTILFTIAGMVPF
                     KPIFLGQKKSVLKRATSSQKCLRTNDIENVGKTARHHTFFEMLGNFSFGDYFKKEAIM
                     WSWEFFTEVIGFPKEKLYVSVYKDDQEAYDLWHNMVGLPEGRIVRLGDEDNFWKVGPE
                     GPCGPCSEIYIDLGPERASVTGGGVGVDERYLELWNLVFMQYNRNQDGSLEPLPSQNI
                     DTGLGLERITSALQNVPSDFETDLFYPIIQYTARKAGIAYGENHKNDIALKVISDHIR
                     ALVFMITDGILPSNEGRGYVLRMLLRRAYRFGKLINFDKPFLYDIAPQVVQNMWQAYP
                     EIKEKEKIVYEIILTEEKRFQETLNLGLSILEEYIQKLERSNQKILSGKDAFTLYDTY
                     GFPLEITRDILSESGLLVDEQGYQKYMLEQKERSRSHQLWGADKVNHQFLEKQVKLYK
                     TIWADNRDTRFCGYAQISTNTRIIALLKGSEPVEQLSSAEEGIIILEETPFYAEKGGQ
                     KGDTGTILGADNSSLAQVFRDDHLRGMWP"
BASE COUNT          881 a          518 c          607 g          840 t
ORIGIN      
        1 tctgccctgg aagccataag ccaggaagcc cccattattc agctggtgaa tatgattatt
       61 gtagatgcaa ttaaagagag ggccagcgat atacatttag agcccaatca gagaggtctc
      121 ttagttcgtt ttagaattga cggtattctt cacgatgtac gcatgctccc gcagagggta
      181 agaccggcag tagtatccag ggtaaaaatt ctttccaata tggatattgc ggaaagaaga
      241 ttgcctcagg atggtaggtt tcaactgcgc ttcggagccc gtgaagtgga cttaagagta
      301 tcctctattc ccactgttta tgacgaaaaa atagtaatgc gtattctgga taagagtcag
      361 ggagtaatag ccctggagag tatcggattt accgaagcac aggtgcagga tttccgtaaa
      421 atgattcatt atcctcatgg cattattctc attaccggac ctaccggcag cggaaagagt
      481 accactcttt atgctacctt aaatgaagta gattctgcta aaaaaaatgt tattactgtg
      541 gaagaccctg ttgagtataa actggataga attaaccaga taaatgtcag gcctaaaata
      601 aacctgacct ttgccaatgc ccttcgttcc attttacgcc aggacccgga cattattatg
      661 attggtgaaa tgagagattc tgatacagcc catatagctg ttcagtctgc cctaaccgga
      721 catctggtct tcagcaccct ccataccaat gatgctgtca gctctttgac ccgtatgacc
      781 gatatgggag tggagccatt tcttatttca tcctcagtca ttggggttat ggctcaaaga
      841 ttggttcgca ggatctgtcc caaatgtata gaggaatata tccctgagga aggcatattt
      901 gatgacctga aactgaatgt tgtcttacct gacaaagaca agataagatt gttcaggggt
      961 aaaggctgtg attactgcaa aaataccggc tattatggaa ggatagcaat ctttgagatg
     1021 atagaaataa atgatgaaat acgttctatg attttgaaaa aaatctcaag taaccagata
     1081 aaagaagttg ccatccgcta tggaatgaaa acccttcttg atagtggaat cgaaaaggct
     1141 ttggaaggta ttaccactat agatgaagtt ttcagggttg cagtatcgta actcccatta
     1201 actattaaaa cattatggaa tttcccatga aaaagatgaa cagtaatcaa attagagata
     1261 cttttttaca atttttccag tccaagggac ataagattat gcccagtgct tctcttattc
     1321 ctgaagaccc tactatttta tttaccattg ccgggatggt tccttttaaa cccatctttt
     1381 tagggcagaa aaaatctgtc ctgaaaagag ctacttcctc tcagaaatgc ctgaggacca
     1441 atgatattga aaatgttggt aaaactgccc gtcatcatac tttttttgaa atgctgggca
     1501 atttctcttt tggtgattac tttaaaaaag aagcaattat gtggagctgg gaatttttta
     1561 cagaagtgat tggtttccct aaagaaaaat tatatgtgtc cgtttataaa gatgatcagg
     1621 aagcatatga cctctggcat aatatggttg gattgccgga aggtcgtatt gtcagactgg
     1681 gggatgagga taatttttgg aaagtcggtc ccgaagggcc ttgtggtccc tgttcggaaa
     1741 tatatattga tctgggtccc gaaagagctt ctgtcacagg tggcggagtc ggtgttgatg
     1801 aacgttattt ggaattgtgg aacctggttt ttatgcaata caaccgtaat caggatggct
     1861 ccctggaacc tttgccctct caaaacatcg ataccggctt aggtttagaa agaattacct
     1921 cagctctgca aaatgttccc tccgattttg aaacagattt attctatcct attattcaat
     1981 atactgcccg aaaagcaggg attgcctatg gtgaaaatca taaaaatgac attgctctaa
     2041 aggttatttc agaccatatt cgagcactgg tttttatgat aacagatggt atacttcctt
     2101 ccaatgaagg aagaggatat gtgttgagaa tgctgctgag aagggcttat cgttttggta
     2161 aattaatcaa ttttgataaa ccttttcttt acgatattgc tccacaggta gtacaaaata
     2221 tgtggcaggc ttatcctgaa ataaaagaaa aagaaaaaat agtctatgaa attatcttaa
     2281 ctgaggaaaa gcgttttcag gaaaccctca atcttggcct ttctatttta gaagaatata
     2341 ttcaaaaatt agaaagaagt aaccaaaaaa ttttaagcgg taaagatgcc tttaccctat
     2401 acgataccta tggcttccct ttagaaatta ccagagatat tctttctgaa tcaggtttat
     2461 tggtagatga gcagggttat cagaaatata tgttagaaca gaaagagcgt tcccgttcac
     2521 atcaattatg gggagcggat aaagtcaacc atcagttttt agagaaacag gtaaaacttt
     2581 ataaaaccat ttgggctgat aatagagata ccagattctg tggctatgca cagatttcta
     2641 ccaataccag aattatagct cttcttaaag gctctgaacc agttgaacag ctttcttctg
     2701 ctgaagaagg aattataata ttagaagaaa cacctttcta tgcggaaaag ggtggtcaaa
     2761 aaggtgatac cggcactatt cttggtgctg ataattcatc actggcacag gttttccgtg
     2821 acgatcacct gcggggcatg tggcct
//
LOCUS       DAOQWT010000171         2842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_37995_length_2842_cov_3.613563, whole genome shotgun sequence.
ACCESSION   DAOQWT010000171 DAOQWT010000000
VERSION     DAOQWT010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37995_length_2842_cov_3.613563"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="PKJ95_04155"
     CDS             complement(<1..173)
                     /locus_tag="PKJ95_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNR65470.1"
                     /translation="MAKKITANIKLQIPAGAANPAPPVGPALGQHGLNIMEFCKAFNE
                     RTKQDIGTIIPVI"
     gene            complement(293..850)
                     /gene="nusG"
                     /locus_tag="PKJ95_04160"
     CDS             complement(293..850)
                     /gene="nusG"
                     /locus_tag="PKJ95_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNR65471.1"
                     /translation="MKGWYKIIRSIEDKKWYVVHTYSGYENKVKANLEQRVKSMGMED
                     QIFSVLIPTERILETKSGKKRYVQKKVFPGYVVVLMKLNNESWYAVRNTPGVTRFVGA
                     GGKPIALEDHEINNILKQMGKGEKKPKIDLDIGTTVNIITGPFTGYTGKVNDIDTQRG
                     KLKVFLTIFGRETSVELEFADVEKY"
     gene            complement(885..1085)
                     /gene="secE"
                     /locus_tag="PKJ95_04165"
     CDS             complement(885..1085)
                     /gene="secE"
                     /locus_tag="PKJ95_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNR65472.1"
                     /translation="MKISQFFQKIVNFIKEARTELKKVTWPNRKQLIGSTIVVMVTVV
                     LVAIYLGLIDLFFSHVVALILQ"
     gene            complement(1128..1203)
                     /locus_tag="PKJ95_04170"
     tRNA            complement(1128..1203)
                     /locus_tag="PKJ95_04170"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1168..1170),aa:Trp,seq:cca)
     gene            complement(1225..1374)
                     /gene="rpmG"
                     /locus_tag="PKJ95_04175"
     CDS             complement(1225..1374)
                     /gene="rpmG"
                     /locus_tag="PKJ95_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNR65473.1"
                     /translation="MRDRIVLACSECNNRNYNNYKNKKNTTAKISLKKYCKFCKKHTL
                     HKEIK"
     gene            complement(1617..2819)
                     /gene="tuf"
                     /locus_tag="PKJ95_04180"
     CDS             complement(1617..2819)
                     /gene="tuf"
                     /locus_tag="PKJ95_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNR65474.1"
                     /translation="MAKEKFVRSKPHVNIGTIGHVDHGKTTLTSAITKYLSGKKLAKA
                     TAFEEIDRAPEERDRGITISVFHAEYETENRHYAHIDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDPELIDLVEMEVRELLNE
                     YEFPGDDIPVIRGSALKAMEADPGNPDDPAFKPIQELLDAVDSYIPTPMRDVDKPFLL
                     SVEDVFSITGRGTVATGRVERGIVKVGDQVEIVGLSDKPKKTVATGVEMFRKLLDQAE
                     AGDNVGILLRGVDKKEVRRGQVIAAPGSITPHTKFKAEVVILTKEEGGRHTSIFSGYR
                     PQFYFRTTDVTGTVYLPEGVEMAMPGDNLTITGELITPIAMEKGVRFAIREGGRTVAA
                     GVVTEIAV"
BASE COUNT          798 a          615 c          470 g          959 t
ORIGIN      
        1 atgataacag ggattatagt accaatgtct tgtttggtcc gttcattgaa agctttacaa
       61 aattccataa tattaagccc gtgctgtcct agggccgggc ctactggcgg tgctggatta
      121 gcagccccgg cagggatttg tagcttaata tttgccgtta ttttttttgc cattttcgtt
      181 tatacccttt cttgcttaat atttgtaatc catttttaaa tccaaataaa tctttataat
      241 aaaattaata acacgttttc cctgatagtt aataaacttg tcacttattt atctaatact
      301 tttctacatc agcaaattcc aactctactg atgtttctcg gccaaaaata gtcaaaaaaa
      361 cttttaattt tcctctttgg gtgtcaatgt cattcacttt tccggtgtag ccagtgaagg
      421 gtccagtaat aatattaacg gtggtgccaa tgtcaagatc aatttttggt tttttctcac
      481 ctttacccat ctgttttaat atattattta tttcgtgatc ttccagggca ataggctttc
      541 ctccggcacc aacaaatctg gtaaccccag gtgtatttct aaccgcatac caggattcat
      601 tatttaactt cattaatact accacataac cgggaaaaac ttttttttgc acatatcttt
      661 ttttcccgga tttcgtttct aaaattcttt ccgttggtat taaaacgcta aatatctggt
      721 cttccatgcc catagattta acacgttgct caagatttgc tttaacttta ttctcatatc
      781 cagaataggt atgaaccaca taccatttct tgtcttcaat agatcttatt atcttgtacc
      841 accctttcaa tagggtttta agagtaacca aacttcaatt tgtattattg taaaataagg
      901 gcaaccacat gtgaaaagaa aaggtcaatt aatcctaaat agatggcaac taatacaact
      961 gtgaccatta ccacaatggt actgccaatt aattgttttc tattaggcca ggtgactttt
     1021 tttagttccg ttctggcttc ttttataaag ttaactattt tttgaaaaaa ttgactaatt
     1081 ttcattttcc accttattaa agctatttgt atttttaagg aattatctgg caggcccggc
     1141 aggatttgaa cctgcaaccc ccggatttgg agtccggtgc tctcccgtta gagctacgga
     1201 cctgcatatc gaactcaaaa tacactattt tatctcttta tgtaatgtat gctttttaca
     1261 aaacttgcaa tatttcttta aagagatttt ggcagttgta ttcttcttat tcttataatt
     1321 attgtaattt cgattattgc actctgaaca ggctagtaca atcctatctc tcatccttta
     1381 atcctcgcta tcagagaaaa tttacgaaac ttaatctaac acattttatc atacctgtca
     1441 agttatcatg ttttattatt ttgtaatcgt aaaaattgaa aataattaaa ttccacataa
     1501 ctgtaaattt ataaaactta acaagaatga caataattaa gactgggatt ttatcccagt
     1561 ctatttattt attttttaaa gaaatacacg tattttttaa tagtattctg attatattat
     1621 accgcgatct cagtaaccac ccctgccgca acagtccttc caccctctcg aatagcaaag
     1681 cgaacaccct tttccatagc aatgggagta atcaattcac cggttatggt tagattatca
     1741 ccgggcattg ccatctctac tccttctgga agatatacgg tacctgttac gtcggtagtt
     1801 ctgaaataga actggggacg atagccgctg aagatagagg tatgtcttcc tccttcttct
     1861 ttggtcagga tgacaacttc tgctttaaat ttggtatgag gagtaatact acccggggca
     1921 gctatgacct gaccacgcct tacctctttt ttatctacgc cacgaaggag tataccaaca
     1981 ttatcaccag cttcagcctg atccagcagt ttgcggaaca tctccacacc agttgctact
     2041 gtctttttcg gtttatcact caatcccaca atctctacct ggtcaccgac tttgactatc
     2101 ccgcgctcca ctcgaccggt agctactgtt cctctgccgg tgatactgaa gacatcctct
     2161 acagagagga ggaagggttt atctacatca cgcatcggtg taggaatata gctatcaaca
     2221 gcatctaata gttcttgaat aggtttgaaa gctggatcat caggattacc cgggtcagct
     2281 tccatagcct taagggcaga acctcttatg accggtatat catcaccggg aaactcatat
     2341 tcattgagta attcccgcac ttccatttct accaggtcaa ttaactcagg atcatctacc
     2401 atgtcaactt tgtttaaaaa gactacgata tagggcactc ctacctgacg agataagaga
     2461 atgtgttccc gggtctgagg cataggacca tcagcagccg agactaccag aattgcacca
     2521 tccatctgag ctgcaccggt aatcatattt ttgacatagt cagcatgacc gggacaatca
     2581 atatgggcat aatgtctatt ttccgtctca tattccgcat ggaatactga gatagtaatt
     2641 cccctgtctc tctcctctgg agcacggtcg atttcctcaa aggcggtagc tttagcaagt
     2701 ttcttaccgg ataaatattt ggtaattgct gaggtaagag ttgttttacc atgatcaaca
     2761 tgaccaatag tcccaatatt aacatgcggc ttggagcgga caaacttttc tttagccatt
     2821 ttgttttttc ctccttaaaa gg
//
LOCUS       DAOQWT010000172         2838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_38083_length_2838_cov_3.490478, whole genome shotgun sequence.
ACCESSION   DAOQWT010000172 DAOQWT010000000
VERSION     DAOQWT010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38083_length_2838_cov_3.490478"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(339..1220)
                     /locus_tag="PKJ95_04185"
     CDS             complement(339..1220)
                     /locus_tag="PKJ95_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNR65475.1"
                     /translation="MEDVGSKTTFEKEMDDIKKEKQKANQEMAEKLEKNMADIEHKIL
                     VLSNKGGVGKSSVAVNLSCALSEKSYKVGLLDADLHGPSVAKMLGFEGEKLTGSNGLI
                     NPIKVRPNLEAISMASLIGSPDTPLVWRGPLKGMAIKQFLAEVNWGELDFLIIDSPPG
                     TGDEPLSICQLIPELDGAVIVTTPQEVALTDSRKCITFLKDLNIPILGIIENMSGFRC
                     PHCGKKIDIFKSGGGKKAADEFQVPFLGSIPIDIEITNSGDQGTPFMCNNKQSESGQA
                     FEKIVQLILDEILKSEK"
     gene            complement(1710..2093)
                     /locus_tag="PKJ95_04190"
     CDS             complement(1710..2093)
                     /locus_tag="PKJ95_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNR65476.1"
                     /translation="MPGGDRTGPLGFGPMSGRGVGFCAGYLTPGYTNPWGGRFGAGFG
                     RGWGRGFGRGRGFRRAAFLGYPSYPAYNVAPPAYPYYYSSYPYQGAMDSKQEMDLLQA
                     EAEDIKAELEAVNTRIAELKKEKKE"
     gene            complement(2359..>2838)
                     /locus_tag="PKJ95_04195"
     CDS             complement(2359..>2838)
                     /locus_tag="PKJ95_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNR65477.1"
                     /translation="KYSGMTAAKVPEHVENYRWLFEGVTGKKISDQDMISQSEAVHNF
                     QRLFNLKMGFGTRKFDAIPYRAMGPVTEQEYRSREDLYDKQLQEEVGYEVEGKDILEK
                     IKTLREYREKQYQLLCDAVYERKGWDKNGVPTLDTIRRLQIDFPEVIDLWQNYNNNK"
BASE COUNT          845 a          583 c          393 g         1017 t
ORIGIN      
        1 taactcctat attttaatta taacgtatac cttaaaaaaa cgatctttac ttttgttatc
       61 tgacagccat aggaacggtt taccagtttg ttgtattaac cagacagaaa aactgtcctc
      121 agggttggtg ctgcagtaca gtcgttgtta ttaaacaagt tttagtttat attttttagt
      181 gaaataattt tggtttaagg ttgctgattt ttagcattag taagaactta tgtattaaaa
      241 atatttctta taattaggtt gctctgaatt tattcttcac aatcgactaa aaactatcaa
      301 ctatgaacct tatccttcac tcctacactt attactattt acttttcact ttttaaaatt
      361 tcatccagaa tgagctgtac tattttctca aaagcttgtc cactttcact ctgtttattg
      421 ttacacataa atggtgtgcc ctgatccccg gaatttgtta tttctatatc aattggtatg
      481 ctgcccagaa atggcacttg aaattcatct gcagcttttt tacccccacc actcttaaat
      541 atatcgatct ttttaccgca atgagggcat cgaaaaccac tcatattctc aattattcct
      601 aatataggga tattgaggtc ctttaaaaag gtgatacatt ttcgagaatc tgttaaggct
      661 acctcctgcg gtgtagttac aattactgca ccatccaatt ctggtattag ctgacatatc
      721 gataatggtt catcgccagt acctggaggg gaatcaatta ttaaaaaatc aagttccccc
      781 cagtttacct ctgccaaaaa ttgtttaatt gccatccctt ttaaaggtcc cctccatacc
      841 agaggggtat ctggtgaacc aattaaagaa gccattgaga tagcttccag attcggtcta
      901 accttgattg gattaattag cccattacta ccggtcagtt tttccccttc aaaacctaac
      961 atcttggcta cagaagggcc gtgaagatct gcatctaata gtccaacctt ataacttttt
     1021 tcagaaagag cgcaggataa attcaccgca accgaacttt tgccaactcc ccctttatta
     1081 gacaaaacca atattttatg ttcaatatca gccatattct tttccaattt ttcagccatt
     1141 tcctgattgg ctttctgttt ttcctttttt atatcatcca tttctttctc aaatgttgtt
     1201 ttactaccca catcctccat ataatatttc cccttgcttg ttttattaat ttttaatttg
     1261 tactcttgtt tattgcttta taatagaaat aagattttta tctctagttt atcctttacc
     1321 ttaaacctgc ctggagatat gttaacaata attctattga acaactcttt agagaacagt
     1381 tttactcgaa ccatttaaga agcattcctc tgtatagatg atcgatagag atggtatggt
     1441 ttccattgcg ccattaccat ccttattttt tgaagcaggt aattgttatt ataccacagt
     1501 ttgagggtaa attacagaaa taagcttctc ctcccactcc taacgttgag aaattgaact
     1561 atacagctta aatacttact accaggcttt taatgttatc cttatcttcc tcaatgatat
     1621 atttacttgt tataataccc aataaaaaaa taaaatcatc aatagatatc ataatctgct
     1681 gatgatttta tttataatta tagttctact tactcttttt tctctttttt taattcagca
     1741 attcttgtat taactgcttc taattcagct ttgatatcct ctgcctctgc ttgtagcaaa
     1801 tccatctcct gcttagaatc cattgctccc tgataaggat aagaagaata ataatatgga
     1861 taagcaggtg gagccacatt ataagctgga taggatggat atcccaaaaa cgcagctctt
     1921 cggaaacctc ttccacggcc aaatccacgt ccccatcctc tgccaaaccc agctccaaaa
     1981 cgaccacccc atggattggt ataaccgggt gtgaggtaac cggcacaaaa tcctacccct
     2041 ctgcctgaca ttggaccaaa acctaaagga ccagttctat caccacctgg cataatattc
     2101 acctccttcg accaattata attattattg aaaatgattg tcattatcat attacaatta
     2161 atatcgactt ttgtcaagag taataaagga gtaaatgaga aaattggtga taaaataatt
     2221 tttttgaaaa attagttatt tggggagcag attacaatag aagtaaaaaa taacaaaggc
     2281 aaaaatttct gcctttgtta ttatattaaa taaactcaaa tatgtaatgt tatttattta
     2341 tctattttat tattagcatt atttattgtt attataattc tgccataggt caatcacttc
     2401 aggaaaatca atttgcaacc ttctgatagt atctaaggta ggtacgccat ttttatccca
     2461 gccttttctt tcatatactg catcacataa taattgatat tgtttttccc ggtattctct
     2521 taatgttttt attttttcaa gaatatcctt tccttccact tcatatccta cctcttcctg
     2581 taattgctta tcatataaat cttcgcggga tcgatattcc tgttctgtta ccgggcccat
     2641 tgcccgataa gggatagcat caaattttct ggtaccaaaa cccatcttta aattaaatag
     2701 tctctgaaaa ttatgtactg cttcagattg acttatcata tcctggtcag aaatcttttt
     2761 tccggttacc ccttcaaata accaacgata gttttctaca tgctccggaa ctttagcagc
     2821 tgtcataccg gaatactt
//
LOCUS       DAOQWT010000173         2837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_38097_length_2837_cov_4.028756, whole genome shotgun sequence.
ACCESSION   DAOQWT010000173 DAOQWT010000000
VERSION     DAOQWT010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38097_length_2837_cov_4.028756"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /gene="iorA"
                     /locus_tag="PKJ95_04200"
     CDS             complement(<1..1623)
                     /gene="iorA"
                     /locus_tag="PKJ95_04200"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNR65478.1"
                     /translation="MKVQDIYEKKQGEKLFLLGNEAAVRGALEAGISFASTYPGTPSS
                     EIGNVLARIAHQVGVYFEFSVNEKVALEVSAAAAAAGLRSFVFMKHVGLNVAADAFMS
                     TAYTGVRGGMIILSADDPSMHSSQNEQDNRIMAQLAGVPVLEPSNPQEMKDFMIYGIN
                     ISEQFKIPVMIRTTTRVSHMRGVVELGEIKPGEKVGFFKKEEPGFTIAPAFARKMRQE
                     LIKKLNQLEVIGNTSQLNKVVRKNNKNKVGIITSGVSYNYVLDTVNKYSLPVNILKLG
                     FCYPFPRELMKNFIANLDKIIVIEEVEPFLEKEVLTIIGQEGWQKKVYGKLDHTLPHI
                     YEYNPDIIEQGLTQVLKVEVAEKNPGIDFSGLTPPMRPPVLCPGCPHRSTIYAIKKAV
                     QGLKIKKENIIYSNDIGCYTLAMQPPYYMADYGICMGSSIGIASGMSKATRQKIVALI
                     GDSTFFHAGIPPLINAVHQKANIMVVILDNEVTAMTGGQPNPGILTDRGGEKSTVVSI
                     EELSRAAGAGYVKTIDPGDLDNSVETFKEGLQY"
     gene            complement(2086..>2837)
                     /locus_tag="PKJ95_04205"
     CDS             complement(2086..>2837)
                     /locus_tag="PKJ95_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNR65479.1"
                     /translation="RVAILTKMSSSDYDAIQKLNASPKNIFLINSKKTTSIRNVYLDE
                     SRENRISTALSVADPFLIEEIPHDLKARVTHFASLIYGEYQPDLLEFFAGKGKIAVDV
                     QGFLRNVGEKGNLIYKSWRDKEKYLPYIDFLKTDAAEAEFLTGHKDTREAAKALHKLG
                     AREIVITHHSEVIAYDGFSFHHYPLKPKNLSGRTGRGDTCFSAYLSERLNKDIEYSLL
                     FAAALTSIKLAKPEPFQGTRGEVENFIKENY"
BASE COUNT          845 a          572 c          469 g          951 t
ORIGIN      
        1 atattgtaat ccctctttaa aggtctccac cgagttatct aaatctccag gatctattgt
       61 cttcacataa cctgctccag ctgccctgct taactcttca atagatacca ctgtgctctt
      121 ctctccaccc ctgtctgtta aaatacctgg atttggctga cctccggtca ttgccgtaac
      181 ttcattatcc agtattacta ccataatatt ggctttctga tggactgcat taattaaagg
      241 tgggataccg gcatgaaaga aggttgagtc tccaatcaga gcaacaatct tttgtctggt
      301 agctttactc ataccgctgg caattccaat actggagccc atacaaatac cataatctgc
      361 catataatag ggaggctgca tagccagagt ataacaacct atatcgttag agtagattat
      421 attttctttt tttattttta aaccctgaac agccttttta atagcataaa ttgtggagcg
      481 atgaggacat cccggacaga gcaccggtgg tctcataggt ggtgttagac ctgaaaaatc
      541 gattcctgga ttcttctctg ctacctctac ctttaatact tgagtaaggc cctgctcaat
      601 tatatcaggg ttatattcat atatatgggg caaagtgtga tccaatttgc catagacctt
      661 tttttgccaa ccttcctgac caataatggt aaggacttct ttttccagaa atggttctac
      721 ctcctcaatc acaataattt tatcaagatt agcaataaaa ttcttcatta attccctggg
      781 aaagggatag caaaaaccca atttaagaat gtttaccggt aaagaatatt tattgactgt
      841 atccagaaca tagttataag aaacaccaga ggtaattatt cctactttat ttttattgtt
      901 cttccttact actttgttca gttgtgaggt atttcctatt acttccagct ggttcagttt
      961 ttttatcaac tcctgcctca ttttacgggc aaaagctggt gcgatagtaa accctggctc
     1021 ttccttttta aagaatccca ctttctctcc gggtttaatt tctcccagtt caacaactcc
     1081 tctcatatgg gaaacgcgtg tagtagtgcg aatcatcacc ggtatcttaa actgttctga
     1141 gatattaata ccatatatca taaaatcttt catttcttga ggattagaag gctctaaaac
     1201 cgggaccccg gccagttgtg ccataatccg gttatcctgt tcattttgag aagaatgcat
     1261 tgaggggtca tctgcagaaa gaattatcat tcctccccgg acaccggtat aagcagtact
     1321 cataaaggca tctgcagcaa catttaatcc aacatgcttc ataaaaacaa aagagcgcag
     1381 tccggctgct gctgcagcag cagatacttc cagggctacc ttttcattta ctgagaattc
     1441 aaaatatact cccacttgat gggcaattct ggctaataca ttacctatct cggaagaagg
     1501 agtacccgga taggtagaag caaaagatat tccagcctct agcgctcctc tgactgctgc
     1561 ctcattcccc aataaaaata gtttttctcc ctgttttttt tcataaatat cctgaacttt
     1621 caaattctca cccctattct tatcttatat ttaatttttt tcatatcatc attttccatt
     1681 atgttaatat atattgaaac catatgattt ccaatattta aggtaacttg aatttatttg
     1741 catttttttc aggaggttta accatactct tagaaaaatt gttttcctta ttattttaaa
     1801 aagaaagtac gccatttttt caatggttaa cgaatcaaaa ataaaaaagg aattttttga
     1861 attgtaaatt gaatattgga agttattgga ttgctccata attgtattat acagattttt
     1921 aatttttaga tggcaaacaa gtttattata acatagatag gattttttgt cctgcagata
     1981 atcagaaaaa gctgattgaa ccgaataata ttcaatgagg ggaagccatt taaaattata
     2041 attcgatcgg aaaaaagagg taatatttct tgataaacaa ggccatcaat agttttcctt
     2101 aataaaattc tcaacttcac ccctggttcc ttgaaaaggt tccggtttag caagtttaat
     2161 agatgttagg gctgctgcaa ataagagaga gtactcaata tctttgttta atctttcaga
     2221 aaggtaagcc gaaaaacagg tatctcctct acctgttctt ccagataaat tttttggttt
     2281 tagaggataa tggtgaaagg aaaatccatc ataagctata acttcactat ggtgtgtaat
     2341 gacaatctcc ctggcaccca atttatgaag cgcttttgcc gcttccctgg tatctttatg
     2401 accggttaaa aactcggcct ctgctgcatc agtctttaga aagtcaatat atggaaggta
     2461 tttttcttta tctctccagg atttgtagat taggtttcct ttttccccca catttcttaa
     2521 aaacccctgc acatcaactg ctattttccc ctttccagca aagaattcaa gtaaatcagg
     2581 ctggtattct ccataaataa ggctggcaaa atgggtaacc cttgctttta aatcatgggg
     2641 aatttcctca attaaaaaag ggtcggcaac agaaagagcc gtacttatcc tattctcacg
     2701 actttcatcg agatagacat ttctgataga agtggtcttt ttagaattta tgagaaatat
     2761 attttttggg gaagcattca atttttgaat agcatcataa tcagaactac tcatttttgt
     2821 taatattgca accctgt
//
LOCUS       DAOQWT010000174         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_38171_length_2834_cov_2.749550, whole genome shotgun sequence.
ACCESSION   DAOQWT010000174 DAOQWT010000000
VERSION     DAOQWT010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38171_length_2834_cov_2.749550"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      325..492
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            569..1498
                     /locus_tag="PKJ95_04210"
     CDS             569..1498
                     /locus_tag="PKJ95_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNR65480.1"
                     /translation="MNKRIFISFTILSIFFITVLSVCGKSSDGIYPITLVDDLGSEVI
                     IEKKPGKIVSTAPSNTEIIFALGLEDKLIGRSDFCNYPPDAEKIESIGVMTPLNLEKI
                     ISLEPDLILAYGGFGTKDIPKIRELGFKVLVIEAESITEMLHSIEFIATACGIPEKGR
                     ELTTSLEERINTVISLHSDIPDIEKPKVFTGSGFETIWSPGSGTIFDELVTLAGGKNI
                     TENQQGWVPISSELVIETQPDIIIIPTGSMNPEEVTKMKNDIIKRPGWSGLPAVKNNH
                     IFVVNEDLLYREGPRLVEGLELLYEIFHKIRKE"
     gene            1467..2525
                     /locus_tag="PKJ95_04215"
     CDS             1467..2525
                     /locus_tag="PKJ95_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNR65481.1"
                     /translation="MKFSTKSEKSNLYGYSNWRYKAILIVVVGFLAILVIGFIGLNFG
                     TIAIQPKKILSILWHHKTDDSLGLIIWELRIPRLIMAMLTGMTLASVGGGFQGILRNP
                     LADPYILGVSAGAALGACSALAVQYTAGLYLVPFLPVFALMGAFVAISLVYLLSRKKS
                     HLPMSDLLLAGVAVNFFFSAIITLLLAISRRETRSMLFWLMGDLSTSSWNNIYWISLP
                     VFLGIILLVSSSLELNAIALGEEEALHLGIQTEKLRLKIFFIGSTMIAIVVSFTGLIG
                     FVGLVIPHIARLLVGPDHRIMLPVSALSGSIFLIICDTIARTIIAPTEVPIGVITALI
                     GSPIFIHLLKRRNENNGY"
     gene            2506..>2834
                     /locus_tag="PKJ95_04220"
     CDS             2506..>2834
                     /locus_tag="PKJ95_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65482.1"
                     /translation="MKIMVTDFKPIVSLEDVTFSYNHHPAIQEMNLSIYPGQFIGIIG
                     PNGSGKTTMLKLISGIITPQRGNIKIFGKDVRKIPRKKMAKIISMVPQESYITYNYKV
                     KEVVFMG"
BASE COUNT          882 a          511 c          539 g          902 t
ORIGIN      
        1 aatactagat accaacgact atcgactaaa atgtcattac gaaggaattt tcttttttgt
       61 cattgcgtgc tggttttagc cagcgtggca atctcattca cctatacccg catctttatc
      121 tttgaatttt cactaaacac caacggctat cgactaacga ccaaatacca acgactattt
      181 aaaattgaat ttttacgtta tatgaattat aataacatag taattagtat ttatataatt
      241 taatagctca aaaccgtttt gttggctatc taatatctaa tattgaaaaa tatataatga
      301 agtagggtga ttaatactct attgccttac actgttctaa caggttaagg gaagtgtggt
      361 gaaaatccac cgcaagcccg ttactgtaaa aggtaccgaa agctgcatta tgccactgga
      421 ttaactcccc gggaaggcgc agcaaataga tagccttaag ccaggagacc tgcctgtaag
      481 aagcttatta cactctttcg tggtcaaagg gtgtcttctt tatttttaga aataaaacta
      541 ttttaaaaac agcactgaaa ggaaaaatat gaataaaaga atatttatta gttttacaat
      601 attatcaatt tttttcatta ctgtcttatc tgtatgtggc aaatcaagtg atggtattta
      661 ccctatcact cttgttgatg accttggttc ggaggtcatt atagaaaaaa aaccgggaaa
      721 gattgtttct accgctccca gtaataccga aattatattt gcccttggtt tagaggataa
      781 attaattggc agaagtgatt tttgcaatta tcctcctgat gcagaaaaaa ttgaatccat
      841 tggagtaatg acacccttaa atttagaaaa aataatatca ctggaaccgg atctaattct
      901 tgcttatgga gggtttggga ccaaagatat tcctaaaata agagagttgg gttttaaagt
      961 tttagtaata gaggctgaat caataacaga aatgcttcac agcatagaat ttattgccac
     1021 tgcctgtggt ataccggaaa aaggaagaga actgactacc agcttagaag aacgaataaa
     1081 tactgtcata tccttacatt ctgacattcc tgatatagaa aaaccaaaag tatttaccgg
     1141 ttctggtttt gaaacgatat ggagtcccgg cagtggaacc atcttcgatg aacttgttac
     1201 cttggcaggt ggcaagaaca ttaccgaaaa tcaacaaggt tgggtcccta tcagttctga
     1261 actggttatc gaaacccagc ctgatatcat aatcattcct accggcagta tgaatccaga
     1321 agaggttact aaaatgaaaa atgatattat taaacgacct ggctggtctg gacttccggc
     1381 tgtaaaaaat aatcacatct tcgttgtaaa tgaagaccta ttatacagag aaggaccccg
     1441 tctggtagaa ggattggaac tattatatga aattttccac aaaatcagaa aagagtaatc
     1501 tatacggtta ttctaattgg agatataaag ctatcttaat tgtagtggtt ggctttttag
     1561 caatattggt aataggtttc atcggactaa attttggcac catagctatt cagccaaaaa
     1621 agattttatc tattctctgg catcataaaa cagatgatag cctgggatta attatctggg
     1681 agttaagaat cccaaggtta attatggcca tgctcaccgg aatgaccctg gccagtgttg
     1741 gaggaggatt tcagggaata ctaagaaatc cattagctga cccctacata ctgggagttt
     1801 cagctggggc tgccctgggt gcctgttctg ccctggccgt gcaatatact gcgggacttt
     1861 accttgtacc ttttttaccg gtgtttgctt tgatgggtgc ctttgttgct atctctttag
     1921 tatacctgct atcacgaaag aaatctcact tacccatgtc agatttattg ttagctggtg
     1981 tagcggtcaa ctttttcttt tcagcaataa tcacattgct tttagctata tcaagacggg
     2041 agacacgttc aatgttattc tggctgatgg gtgacctctc tacttccagc tggaacaata
     2101 tttattggat ttctttaccg gtatttctgg gtatcatcct tttagtatct tcttctttag
     2161 aattgaatgc tatagcactg ggagaagagg aagctctgca cctgggaatt cagactgaaa
     2221 agctaagatt aaaaatattt tttattggtt ctactatgat tgccattgta gtatccttta
     2281 ccggtttgat tggttttgtg ggattagtaa ttccccatat tgctcgccta ttggtaggtc
     2341 ctgaccatcg cattatgcta ccggtatcag cactgagtgg ttctatcttc ttaattatat
     2401 gtgacaccat agcacgtact attatcgctc caaccgaagt acccattgga gtaatcactg
     2461 cccttattgg ttcaccaata tttatccatc tgcttaaaag gaggaatgaa aataatggtt
     2521 actgatttta aacctattgt ttctttagaa gatgttacct tttcctataa tcatcatcct
     2581 gcaattcaag agatgaattt atctatatat cccggacaat ttattggaat tatcggacct
     2641 aatggttctg gtaagacaac aatgttaaaa ttgatatcgg gtataattac ccctcaaaga
     2701 ggcaatatca aaatattcgg gaaggatgtc aggaaaatac ccagaaagaa aatggctaaa
     2761 ataatctcta tggtaccaca ggaatcttat attacctaca attacaaggt taaagaagtt
     2821 gtctttatgg gaag
//
LOCUS       DAOQWT010000175         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_38282_length_2829_cov_2.992430, whole genome shotgun sequence.
ACCESSION   DAOQWT010000175 DAOQWT010000000
VERSION     DAOQWT010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38282_length_2829_cov_2.992430"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKJ95_04225"
     CDS             <1..201
                     /locus_tag="PKJ95_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65483.1"
                     /translation="SLISDISMVLSNHKAGIKYIKGVANKNNVAIINLTIAVNTLDHL
                     KEIMARLRSLKAVKEVIRMEGD"
     gene            214..660
                     /gene="dtd"
                     /locus_tag="PKJ95_04230"
     CDS             214..660
                     /gene="dtd"
                     /locus_tag="PKJ95_04230"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNR65484.1"
                     /translation="MKAVIQRVSRASVRMEGREIARIGEGILVLLGVKQEDTKREAEL
                     LANKTANLRIFNDEQEKMNLSLLDIKGEALVVSQFTLYGNCKKGRRPSFIDAALPEKA
                     DLLYRNYVQYLKDYHITVQTGQFQAMMQVELVNEGPVTIILDSAEL"
     gene            687..1334
                     /locus_tag="PKJ95_04235"
     CDS             687..1334
                     /locus_tag="PKJ95_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR65485.1"
                     /translation="MNKDNKNIYLKKIIMGPLATNCYLLSCKETKEAVVIDPAEESDV
                     LLQLIDGNGLLLKYIINTHGHGDHIGGNKILKEKYSAKLLIHKLDEKMLIEPKKNFSF
                     YTGIPLKSPLPDEYLQDGMVLEVGTLRLFIIHTPGHSPGGVSIKVDDMIFTGDTLFRE
                     SIGRTDFPGGSMPDLMNSIKEKLFIFDGSCEVLPGHGPATTLIHELQNNPWLKLK"
     gene            1373..2629
                     /locus_tag="PKJ95_04240"
     CDS             1373..2629
                     /locus_tag="PKJ95_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNR65486.1"
                     /translation="MSYDIKDISLAESGLRRIKWAQSQMPVLKNIKKRFEAEIPLKNI
                     KIAACLHVTTETANLMIALKAGGANPVLCASNPLSTQDDVAASLVQHYEIPVFAIRGI
                     DQKGYYNHISRTLDCEPNITMDDGADLVTMVHNRRKELIDNIIGGTEETTTGVIRLRS
                     MEKEGVLQYPIIAVNDALTKHLFDNRYGTGQSTIDGLFRATNILLAGKIMVICGYGWC
                     GRGIANRARGMGARVIITEIDPLKALEAIMDGFQVNSMKEAAEIGDIFITSTGNKNVI
                     SKHDFLKMKNGAILANAGHFNVEIDIPALQELAVKTEVMRDGIVSYILKDEKQLFLLF
                     EGRLLNLAAAEGHPASVMDMSFANQSLSIEYLNKEKGSLSKMVYSVPQDIDKEVAFLK
                     LKSMGIEIDVLTDQQKKYLNSWQEGT"
BASE COUNT          987 a          391 c          599 g          852 t
ORIGIN      
        1 agtttaatct ctgatatatc aatggttctt tctaatcata aagcaggaat taaatatatt
       61 aagggtgttg ccaataaaaa caatgtggct ataattaatt taactattgc ggtaaacacg
      121 ctggaccatt taaaggagat tatggctcgt ctgaggagtc ttaaagctgt gaaagaagtt
      181 ataagaatgg aaggagattg aaaggagtag attatgaagg cagtaatcca gagggtaagc
      241 agggcttcag taaggatgga aggaagagag attgcccgaa ttggtgaggg aatactggtt
      301 ttattgggag taaaacaaga ggacactaaa agagaagccg aattactggc caataaaaca
      361 gcaaacttac gtatctttaa tgacgaacag gagaagatga acttatcttt gttagatata
      421 aagggtgaag ctttagtagt atcacaattt accctttatg ggaattgcaa gaaaggcaga
      481 agacccagtt tcatagatgc tgctttacca gaaaaagctg atttacttta tcggaactat
      541 gtgcaatact taaaagatta ccatattact gttcaaacgg gccaatttca agctatgatg
      601 caggttgaat tagtcaatga aggaccggtt actattattc tggatagtgc tgagctgtaa
      661 agtattaaat aatttaggga tagattatga acaaagataa taaaaatatt tatttaaaga
      721 aaatcattat gggaccactg gctactaatt gttatcttct tagttgtaaa gaaactaaag
      781 aggcagttgt tattgatcct gcagaagaaa gtgacgttct tttacagtta atagatggaa
      841 atggactcct tcttaaatat attattaata cacatggcca tggcgaccat attgggggta
      901 acaaaatact taaagaaaaa tattcagcaa aactattaat acataaatta gatgaaaaaa
      961 tgctgataga acctaaaaag aatttttcat tttatacggg aatacctctt aaatcccctc
     1021 tgcctgatga atatctgcaa gatggaatgg ttttagaagt gggaaccctg cgacttttta
     1081 ttatacatac acccggacat tcacccggag gtgtctctat caaagtggat gatatgattt
     1141 ttacagggga tactttattt agagagagca taggccgtac agactttccg ggaggctcta
     1201 tgcctgattt aatgaactct atcaaagaaa agttatttat ttttgatggt tcctgtgaag
     1261 ttttgcccgg ccatggacct gctacaactt tgatacatga attacagaat aatccatggt
     1321 taaaattgaa atagtgtaag ttcaatatat tttaaaggtg gaggaaatta tgctgtccta
     1381 tgatattaag gatattagtt tagccgaatc cggtttgaga cgaataaaat gggcccaaag
     1441 ccaaatgcca gtactgaaga atataaaaaa acgttttgag gctgaaatac cattaaagaa
     1501 tataaaaatt gctgcctgtc ttcacgtaac tactgagact gcaaatctga tgattgcttt
     1561 aaaggcaggt ggggctaatc ctgttttatg tgcttctaat ccgttaagta cacaggatga
     1621 tgtggctgca tctttagtgc agcattatga aatacccgtt ttcgctataa gaggaattga
     1681 tcaaaaagga tattataacc atatcagcag aaccctggat tgtgaaccaa atatcactat
     1741 ggatgatgga gctgatctgg ttaccatggt acataataga agaaaagagt tgattgataa
     1801 tattattggt ggtacggaag aaactaccac cggtgttatt cgtttaagaa gtatggaaaa
     1861 ggaaggcgtt ttacaatatc ccattattgc tgtaaatgat gccctaacca aacacctttt
     1921 tgataacaga tatggaacag gtcagagtac tatcgatggt ttatttaggg cgactaatat
     1981 cttgttagca ggtaaaatta tggtaatatg tggttacggt tggtgtggta gaggaatagc
     2041 caatagagcc agaggaatgg gagccagagt gattattact gaaattgatc cgttgaaagc
     2101 tcttgaggcg attatggacg gattccaagt aaatagtatg aaagaagctg ccgaaattgg
     2161 tgacatattt ataacttcaa ctgggaataa aaacgttatt agtaagcacg attttttaaa
     2221 aatgaaaaat ggggccattc tggctaatgc aggccatttt aatgtagaaa ttgatatacc
     2281 tgcattacaa gaattagcgg taaaaacaga ggttatgcgg gacggtatag tttcatatat
     2341 tttaaaggat gaaaagcaat tatttttgtt atttgaaggc agactgttaa atcttgcagc
     2401 agctgaaggt cacccagcca gtgttatgga tatgagtttt gccaatcaat cattgtcaat
     2461 tgagtattta aataaggaga aaggctcctt atctaaaatg gtctattctg ttcctcaaga
     2521 tattgataaa gaggttgctt ttttgaagtt aaaaagtatg gggattgaaa ttgatgtact
     2581 tactgaccag caaaaaaagt atttaaatag ctggcaggaa ggtacttaga atatttaatt
     2641 ccttatataa gaatacagaa aaaaattaat ctataaaaaa aatgactgat aataattttt
     2701 gggaatacct gaaagaaggt tttcttagcc ttatttatcc acttaattgt gaaaattgta
     2761 gccggccaat tagagagtca aaaggttata gcatttgtga tgattgtctt cagcaaatca
     2821 aatttattt
//
LOCUS       DAOQWT010000176         2820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_38480_length_2820_cov_2.444846, whole genome shotgun sequence.
ACCESSION   DAOQWT010000176 DAOQWT010000000
VERSION     DAOQWT010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38480_length_2820_cov_2.444846"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="PKJ95_04245"
     CDS             complement(<1..1385)
                     /locus_tag="PKJ95_04245"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNR65487.1"
                     /translation="MIEITINPGAKIINVPSGTLLLDVLKQAKISIVTTCGGQGSCGK
                     CRVKVSSTAIKGSLLNLTEKKQLSEEEIKEGFRLACQCHLYHNSTITIPSSVLMRNNN
                     SKIEEKSISHLSHYEFKPVIPIKKYFLRLNPPTLKNQSSDWTRIKDELNRVAKIKKER
                     WFIAPDILGNVSQLLRDNNYGMTIVLYKNEVISVEPGNTSKEMYGVAFDIGTTTIAGY
                     LIDFSTGKQIVAEAGSNPQYIYGDDVISRVGFAGKNATNRNIIQQELISFLNQIITSL
                     SRKTGINLSRIYFAVLVGNTAMHHFLWSLPTENLAFSPYIPVLTEGFFKEARDLPDLC
                     LIPHAKVYSTPNVSAYIGGDIIADLIDINIWGKNNNILMVDLGTNGEIVLKGDDKIWA
                     CSAAAGPAFEGARISSGMRAIRGAIDRIIITEHKIRYQVIEQTRAAGLCGSGIIDLIA
                     SLLKLQLIKSDG"
     gene            complement(1449..2369)
                     /locus_tag="PKJ95_04250"
     CDS             complement(1449..2369)
                     /locus_tag="PKJ95_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNR65488.1"
                     /translation="MEKENKKGMGNDFLALLSQKTLVIDGAMGTMLQQYGLKAGEAPE
                     MWNITQPDIVKSIHTSYLEAGADIILTNTFGANGIKLQKLGFRERLLELNEQAVKLAR
                     KAADEYREVHQQPIFIAGSIGPSGEILGPCGLVKDEEAYAGYEEQVKVLASSGIDFIL
                     LETFYDLNEIGIALKAVKEICDLPVFASMTFDDSLKTIYGISPEKAVKVLFQEGADGV
                     GANCGSGPETLYKVIAQMRVLTDAPLLVKPNAGIPYIDNNRIIYPATPQEMAEYTEKF
                     IQLRVNIIGGCCGTTPEHIRAIAGKVKKSV"
     gene            complement(2490..>2820)
                     /locus_tag="PKJ95_04255"
     CDS             complement(2490..>2820)
                     /locus_tag="PKJ95_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNR65489.1"
                     /translation="GLKEKHRIVCGTNTIEIHYQIHLNNGDYRFFDHVYASHLLGFAK
                     PEPQFYQYIVDKERIGLDRIIFIDDSLENVIAARKFGIKSIHFVDTYSLKNTLTNYSH
                     VNGHKII"
BASE COUNT          828 a          586 c          442 g          964 t
ORIGIN      
        1 ccatcggact tgattaattg taatttcagt aagctggcaa tcaggtcaat tattcctgaa
       61 ccacataatc ccgcagctcg agtctgttca ataacctgat accttatttt gtgttcagta
      121 attattatcc tatctattgc ccccctgata gctctcatgc ctgaactgat tcttgctcct
      181 tcaaaggcag gtccagcagc cgctgaacag gcccaaatct tgtcatcacc ctttaagact
      241 atctcaccgt tagttcctaa atctaccatc agtatgttat tgttttttcc ccatatattt
      301 atatctatca ggtcggcaat aatatcacca ccaatatagg ctgatacatt cggagtacta
      361 tatacctttg catgaggaat aaggcataaa tcaggcaaat ctcgggcttc tttaaaaaac
      421 ccctcagtta aaacagggat atagggagaa aatgccagat tttccgtagg taaagaccat
      481 aaaaaatggt gcatagctgt attgcccacc aatactgcaa aataaatccg agaaaggtta
      541 atccctgtct ttcgcgacag ggatgtaatt atctgattta agaaagatat taattcctgc
      601 tgaattatat ttctattggt agcatttttt ccggcaaaac caactcgaga aattacatca
      661 tcaccataaa tatactgagg gttgctacct gcttcagcta caatctgttt accggtacta
      721 aaatctatta gatatccggc aatcgtggtg gtccctatat caaaagccac cccatacatc
      781 tctttgctgg tattccctgg ttcaactgaa ataacttcat tcttataaag aacaatggtc
      841 ataccataat tattatccct caataattga gatacattgc ctaaaatatc tggtgctata
      901 aaccatcttt cttttttaat ttttgctact ctgttcagtt catcctttat tctggtccaa
      961 tcagaagact ggttttttaa agtaggagga ttgagtctaa gaaagtattt tttaataggg
     1021 ataactggtt taaattcata atgggataaa tgagaaattg atttctcttc gattttagaa
     1081 ttgttgttcc tcatcaatac agatgatgga atggtaatgg tactattatg atacagatga
     1141 cattgacagg ccaggcgaaa cccttctttt atctcttctt ctgaaagttg tttcttttct
     1201 gttagattga gcagactgcc tttaatagct gtagaagata ctttaactct gcatttacca
     1261 cagcttccct ggccaccaca ggtcgtaaca atactaatct tggcctgttt aagcacatcc
     1321 agtaatagtg ttcctgaagg aacattgatt atctttgctc cgggattaat tgtaatttca
     1381 atcatctttt tacattattc attggtatga ttatttcgga agagatgttg ttattatact
     1441 ttactgcttt atactgattt ctttaccttc ccagcgatgg ctctgatatg ttccggggtt
     1501 gttccacaac atcctccaat aatatttact ctcagttgaa taaatttttc tgtatattct
     1561 gccatctctt gtggtgtcgc gggatatata atccgattat tatctatata agggatccca
     1621 gcattgggtt tgaccaatag cggagcatca gttaataccc tcatctgtgc aattacctta
     1681 taaagagtct caggtccact accacaattg gcacccacac catctgcacc ttcttgaaat
     1741 aatactttga ctgccttttc tggacttatt ccatatatcg tttttaaact atcatcaaag
     1801 gtcatagagg caaaaaccgg taaatcacat atttctttaa cagccttcaa ggcaatacct
     1861 atctcgttaa ggtcgtagaa ggtctccaat agaataaaat caattccaga ggaggctaat
     1921 actttaacct gctcttcata gcctgcgtaa gcttcttcat cttttaccaa tccacatggt
     1981 cctaaaattt caccactggg acctatggaa cctgctataa aaatgggttg ctgatgaact
     2041 tctctatatt catcagcagc ttttctggct aatttcacag cctgctcatt taattctagt
     2101 agcctttccc tgaaaccaag tttctgtaat tttatgccat ttgccccaaa agtattggta
     2161 agaataatat ctgcaccagc ttccagatag gaagtatgta tactttttac tatatcaggt
     2221 tgagtaatat tccacatctc aggagcttct cccgctttta aaccgtattg ttgcagcata
     2281 gtgcccatag ccccatcgat gactaaagtt ttttgactta acagagctaa aaaatcattc
     2341 cccattccct ttttattttc tttctccatc tggaatacct cccttactta ttttactttt
     2401 taattgatat tagcaatccc tttcaaattt atcttgatta tcttttttac cttaagagat
     2461 aatatcatat cagaaaatta acttcattgc tatattattt tatgaccatt aacatgggaa
     2521 tagttggtta atgtattctt cagggaataa gtgtcaacaa aatgaatgga tttaatccca
     2581 aattttctgg cagcaatcac attttctaaa gaatcgtcaa taaaaataat cctatctaag
     2641 ccgattctct ccttatccac aatatactga taaaattgtg gttcaggttt agcgaacccc
     2701 aatagatgag aggcgtaaac atggtcaaaa aagcggtaat ctccattgtt aagatgaatc
     2761 tgataatgaa tttctattgt atttgttccg cagacaatgc gatgtttctc ttttaatcct
//
LOCUS       DAOQWT010000177         2771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_39537_length_2771_cov_2.370029, whole genome shotgun sequence.
ACCESSION   DAOQWT010000177 DAOQWT010000000
VERSION     DAOQWT010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39537_length_2771_cov_2.370029"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..723
                     /gene="purN"
                     /locus_tag="PKJ95_04260"
     CDS             94..723
                     /gene="purN"
                     /locus_tag="PKJ95_04260"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNR65490.1"
                     /translation="MEKKIMDMINIAVLVSGRGTNLQAIINVINRGGIDGKIRIVLSD
                     NPEAYALKRAQRNGIKTKVVHYKEYRSKDEYEQEIMKYIDNYRIDLIVLAGYMRILSP
                     NFIKKYPYRIINIHPALLPSFPGLNAQRQAIEYGVKISGCTVHFVDEGVDSGPIILQK
                     AVEVKDSDDEKSLSRRILKYEHRLLPRAIQLFAQNKIQIINKTRVHIKK"
     gene            790..1755
                     /locus_tag="PKJ95_04265"
     CDS             790..1755
                     /locus_tag="PKJ95_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNR65491.1"
                     /translation="MKIVILDGYTLNPGDLNWQGFKKFGDLTIYDRTSLTDEDEIISR
                     ISDAEIVFTNKTPIKANTINSCPNIKFIGVLATGYDVVDIDVARGKGIPVTNIPTYGT
                     SSVGQFAIALLLEICHHIGHHNQAVHDGRWESSPDWCFWDYPLIELDGKSMGIIGFGR
                     IGQNTGTIARALGMKVLANDAIQNDIGREIATYVELDELFHKSDIIALHCPLLPSTRG
                     IINKENISKMKDGVIIINNSRGPLIVEQDLSDALNIGKVYAAGIDVVSSEPIKGDNPL
                     LKAKNCIITPHISWAPKESRKRLLDIAVDNLAHFVKGSPINVVNK"
     gene            1810..>2771
                     /locus_tag="PKJ95_04270"
     CDS             1810..>2771
                     /locus_tag="PKJ95_04270"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNR65492.1"
                     /translation="MKIVIAPDSFKGSLSAAEVSEAIAYGVRGVYPYAIVEKVPMADG
                     GEGTVECLVNATQGKIYEREVLGPLAEPIVATFGILGNQHTAVIEMAAASGLPLVPLE
                     KKNPLITTTYGTGQLIESALDYRCQEIIIGIGGSATNDGGAGMVQALGVHLLDENEKE
                     IGFGGTQLSHLNRIDISEIDKRIRDVSFLVASDVQNPLCGPTGASNVYGPQKGATKEM
                     VKILDDALSHFADIIKRDLGKDIKDIPGAGAAGGLGAGLMAFLDAELKPGIDIVIETV
                     KLEQKLKDADLVITGEGEINASTIYGKTPIGVARVAKKYNIPVVS"
BASE COUNT          941 a          449 c          604 g          777 t
ORIGIN      
        1 aaatgaattg accacccaga aactgaattg ctaaccgaaa tttgtatgaa tatagataca
       61 atttataatt taaataaata tttatagaat taaatggaaa agaagataat ggatatgatt
      121 aatattgcag ttttagtatc cggtagagga accaatttac aggcgattat taatgttata
      181 aatagaggtg gtattgatgg aaagataaga atagtgctca gtgataatcc tgaggcttat
      241 gctttaaagc gggcgcaaag aaatggcatt aaaaccaaag ttgttcatta taaagagtat
      301 cgcagcaaag atgaatatga acaggaaatt atgaaatata ttgataatta caggatagat
      361 cttattgttc tggctggata tatgcgtata ttaagtccta attttataaa aaaatacccc
      421 tatcgtatta taaatattca tcctgctctg ttaccctctt tccctggtct taatgcccag
      481 aggcaagcca tagagtatgg ggttaaaata agtggatgta cggtacactt tgtagatgaa
      541 ggagtagata gcggaccaat aattttacaa aaagcggtag aagtaaaaga ttctgatgat
      601 gaaaaatccc tttctagaag aatactaaaa tacgagcaca ggcttctgcc cagagccatt
      661 cagttatttg ctcaaaataa aattcagata attaataaga ccagagtgca tattaaaaaa
      721 taattattaa ggcatcgtct tgaaaagttt ctatagctta gctagtaaat gctaaaaaag
      781 gagaaaaaaa tgaaaatagt aatattagat ggatatactt taaacccagg tgatctaaat
      841 tggcagggat ttaaaaaatt cggagacctt accatatacg atcgtacctc attaacagat
      901 gaggatgaga ttatctcccg cattagtgat gctgagatag ttttcaccaa taagaccccc
      961 ataaaagcaa atactataaa ctcctgccct aatataaaat tcataggagt acttgctacc
     1021 ggatatgatg ttgttgatat agatgtagcc agaggaaagg gtatccctgt caccaatatc
     1081 cccacctatg gcacctcatc tgtaggacaa tttgccatcg ccctgctttt agagatatgt
     1141 caccacatag ggcatcacaa tcaggctgtt catgatggac gatgggaatc aagtcccgac
     1201 tggtgtttct gggactatcc tttaattgag ctggatggaa agagtatggg tattattggt
     1261 tttggaagaa tcggacagaa taccggtact attgccagag ccttagggat gaaagtcctg
     1321 gccaatgatg ctattcagaa tgatatcgga agagagatag ctacctatgt ggaactggac
     1381 gaattattcc ataaatctga tatcatagcc ttacattgtc ccctgcttcc ttcaactcga
     1441 ggtatcataa acaaagagaa catctctaaa atgaaagatg gtgtgataat catcaataat
     1501 agccgtggcc ctcttatagt ggaacaggac ctgtcagatg ccttaaatat tggcaaagtt
     1561 tatgctgctg ggatagatgt ggtaagcagt gaaccaatta aaggagacaa tccgctactg
     1621 aaagcaaaaa actgtattat caccccacat atctcctggg cccctaaaga aagtcgaaag
     1681 aggctgctcg atatagcagt agataattta gcccattttg taaaaggctc acctattaat
     1741 gtagtaaata agtaataaaa ctattataat ttaattgact ttatataata taaaaaaaga
     1801 gtttgaaata tgaaaatagt tattgcccct gattctttta aaggtagtct gagtgcagca
     1861 gaggtttctg aagcaatagc gtatggggta agaggtgttt atccctatgc catagtagag
     1921 aaggtaccta tggctgatgg gggagaaggt actgtggagt gtttagtaaa tgccactcag
     1981 ggtaaaatat atgagcgtga agtattagga cctcttgccg agccgatagt ggcaacgttt
     2041 ggcattttgg gtaatcaaca tactgcagtt atagaaatgg ctgctgcctc aggacttccc
     2101 ctggttcctt tggagaaaaa aaatccttta attactacaa cttatggtac cggtcagtta
     2161 attgagtctg cacttgatta cagatgtcaa gagataatta ttggtattgg aggaagtgca
     2221 actaatgatg gaggggcagg gatggtacag gctttaggag tacatttact cgatgaaaac
     2281 gagaaggaaa taggatttgg ggggacacaa ctatcccatt taaatcgaat agatatttct
     2341 gaaattgata aacggattag ggatgtatcc tttctggttg cctcagatgt acagaaccct
     2401 ttatgtggac ctaccggcgc ttcaaacgta tatggtcccc aaaaaggtgc taccaaagag
     2461 atggttaaga tactcgatga tgctctgtcg cattttgccg atattataaa acgtgacctg
     2521 ggcaaagata ttaaagatat tccgggagct ggtgctgcag gtgggttggg agccgggttg
     2581 atggcttttt tagatgctga gctcaagcct ggtatagata tcgtgataga aacagttaaa
     2641 ctagagcaaa aattaaaaga tgccgatctg gttatcaccg gcgaaggaga aatcaatgcc
     2701 tctacgatat atggtaaaac acctattgga gtagccagag tagcaaaaaa atataatata
     2761 ccggtagtgt c
//
LOCUS       DAOQWT010000178         2769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_39570_length_2769_cov_2.959101, whole genome shotgun sequence.
ACCESSION   DAOQWT010000178 DAOQWT010000000
VERSION     DAOQWT010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39570_length_2769_cov_2.959101"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..765)
                     /locus_tag="PKJ95_04275"
     CDS             complement(223..765)
                     /locus_tag="PKJ95_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HNR65493.1"
                     /translation="MNEELGFVNPEQELKRAAELMALSARTAPKSAGKDFVVIKIISG
                     EEVVKLGEAMIRYGMEKNKRNFDRDGENVKNSPVLLLIGLKDAQTMGLDCGACGFDLC
                     SECVSHEGPEFDGPQCALRILDMGIAIGSAVKMASVLGIDNRIMYRIGVAAKKYGFIE
                     ASFVMGIPFSVTGKSIYFDR"
     gene            complement(859..1866)
                     /locus_tag="PKJ95_04280"
     CDS             complement(859..1866)
                     /locus_tag="PKJ95_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR65494.1"
                     /translation="MNASICKIRQIRVGKLMDIRVEIDNIIRKIKQHSSEKSVIVAFS
                     GGLDSSVVLFLTSEALGRERIKAVNVDFGLFTYQKARLNVASICEEMQIELDTISGEV
                     YQREMMKGGPDCNLCTRKIKLGLIRACAGNKLILTGSNQSDSWGKYGTDFSSGFFAPL
                     FSYSKEEILQMAGFLNLKIERIGENIYREGCKLKHLLKPLANTSYHGRAVSLANEMLL
                     ELIKELNLHTEIANVKIIGPLNENIALINISPLPAEVRYLEIITDNIASIGEIDSCVL
                     VSHPLQLTIKANKGQFNNLRSRYWIEQGRLQPEFTAPLEFKWLLSTNHSLKTFQIVAY
                     N"
     gene            complement(2126..2686)
                     /locus_tag="PKJ95_04285"
     CDS             complement(2126..2686)
                     /locus_tag="PKJ95_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HNR65495.1"
                     /translation="MILEVLAKRKRMGSFLFLIVIALCFVVLNSVNYADETLEKEEKV
                     LSWLPYEQALAKSKIENIPTLLYFYSDNCGWCRKFEEETLTNREVRELLNKGYALAKI
                     NGNSNQTVMVEGKKFTESRISAEVYQVNAFPTIWFLNYDNERIANLPGFVPSDMFLPI
                     LIYIRDDFYKKYTFQEFLELEKNKQK"
BASE COUNT          846 a          557 c          406 g          960 t
ORIGIN      
        1 taaagacata aagacaggta aaacatttac aagaaggaag cattataatc ttttttgtaa
       61 cctttatatt tatcaatttt tcttttctat ataatctata taatctggat tattacaagt
      121 aatcccggac tgtgcatttt ttgcacagtt tttaatatta tgtaagaaat attttcacac
      181 ctgttttttt taagaaaata tttaactatt tgtttctgct ttttatcggt cgaaataaat
      241 acttttaccg gttaccgaaa aaggaatccc catcacaaaa cttgcttcaa taaaaccata
      301 ctttttggct gctactccaa tacggtacat tattctgtta tcaataccta aaactgaagc
      361 catctttact gctgagccga tggctatccc catatccaga atacgcaagg cacactgagg
      421 gccatcgaac tcaggacctt catggctcac acattcagaa cataagtcaa atccacaggc
      481 accgcaatca aggcccatag tttgagcatc ttttaaacca atcagtaaca gtacagggga
      541 atttttcaca ttttcaccat cacggtcaaa attccgttta ttcttttcca taccgtaccg
      601 tatcattgcc tcgcccagtt tgactacctc ttctccgcta attattttaa ttactacaaa
      661 atctttgcct gccgatttcg gcgctgttct ggcagaaagt gccatcaatt ctgctgccct
      721 ttttaattcc tgttcgggat ttacaaagcc taattcttca ttcataagat actccttaat
      781 tattttattt aaatcctgcg gctaattaat tcagaaaata ctatttcatt ttaacgtcaa
      841 ttcttatcta ttttttcttt aattataagc aacaatctgg aaggttttca agctgtgatt
      901 ggtacttaat agccatttaa attcgagagg ggcagtaaat tccggctgta atctaccctg
      961 ctcaatccaa tagcgtgaac gtaaattatt gaattgacct ttatttgctt ttatggttag
     1021 ctgtaaaggg tgagaaacta agacacagct gtcaatttct ccgatactgg caatattatc
     1081 agtaataatt tctaaatacc gaacttcagc cgggagaggg gagatattga taagggcaat
     1141 attttcattt aaaggaccga taatcttgac attggctatt tcggtatgaa gattcaattc
     1201 ctttatcagc tctaataaca tctcattggc taaactgact gcccttccgt gataggaagt
     1261 attagccagg ggtttgagca ggtgctttaa tttgcaacct tcccggtaaa tattttctcc
     1321 aattcgttca atctttaagt ttaaaaatcc tgccatctgt aaaatttctt ccttagagta
     1381 agaaaacagg ggggcaaaaa atcctgaaga aaaatcggta ccatattttc cccagctatc
     1441 actctgattg gagccggtaa gaatgagctt atttccggca caagctctta ttaaacctaa
     1501 ttttattttt ctggtgcaca aattacagtc cgggccgccc ttcatcattt ctctttgata
     1561 aacctctcct gaaattgtat ccaattctat ctgcatctcc tcacatatcg aagcaacgtt
     1621 cagccgagct ttctggtagg tgaataaacc aaaatcaaca tttactgcct ttattctttc
     1681 tctgcccaga gcctcgctgg ttaagaacaa tactactgaa ctatccaaac ctcccgaaaa
     1741 agcaacaata acactttttt cagaactatg ttgtttaatt tttctgataa tattatctat
     1801 ttcaactctt atatccatca atttaccaac cctaatctgt cttattttac aaatggatgc
     1861 gttcacttac caggagtggc tttaagaata aatatggaat gatacttctt aatattgttt
     1921 gtaaggtggc tttttatgat tagagaaata attgtttttc aggtcaggtg attagttttt
     1981 tactacagtt aattgatatg cattattttt acaggaggat tgtcttacga atttaaaacg
     2041 gttattgctc tattttataa atctacatac tatctcttaa cacatttaaa aataaatctg
     2101 acatctggta ttcagccaat accagctatt tctgtttgtt cttctctaat tccaaaaact
     2161 cctgaaaagt atatttttta tagaaatcat ctctgatata aataaggatc ggtaaaaaca
     2221 tatcagatgg cacaaatcct ggtaaattcg caatcctttc attgtcatag ttcaggaacc
     2281 agatggtggg aaaagcgttg acctgataca cttcagctga aatacgactt tctgtgaatt
     2341 ttttcccctc aaccattaca gtctgattag aatttccgtt aattttagcc agagcatacc
     2401 ccttatttaa taattccctg acttctctgt tggtaagagt ctcttcctca aattttctgc
     2461 accaaccgca attatcagaa tagaaataga gcaaagtggg gatattctca attttggatt
     2521 tagccaatgc ctgttcatag ggcagccagg acagaacttt ttcttctttc tctaaggtct
     2581 catcggcata attaacagaa tttaaaacaa caaaacaaag agctattaca attaaaaaaa
     2641 gaaatgagcc cattctcttt cttttagcta atacttctaa aatcattatt tagcccacct
     2701 taattataat gtttttgaat tctttttatt atactatcta ttaacctgct aagattatca
     2761 tctttttct
//
LOCUS       DAOQWT010000179         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_39615_length_2767_cov_2.813791, whole genome shotgun sequence.
ACCESSION   DAOQWT010000179 DAOQWT010000000
VERSION     DAOQWT010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39615_length_2767_cov_2.813791"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PKJ95_04290"
     CDS             <1..582
                     /locus_tag="PKJ95_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNR65496.1"
                     /translation="IAKEMGFEVKHQPTAWDGIITALLAKKIDMVYSGMSITPERLEK
                     VNFSIPYWEIDQALCVRKDSDLNVIAALCGKYVTGSQRGCTAAEWIEANLVNTGIIPK
                     ENFKLYETFSLAVQDLVNGRLDSAMMDDVMVEDAIRSGQPVKIVGIIKTGEAYGVAIR
                     KEDKELEKMLNEGIKKLLKADIWEELKIKYLTD"
     gene            714..1391
                     /locus_tag="PKJ95_04295"
     CDS             714..1391
                     /locus_tag="PKJ95_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNR65497.1"
                     /translation="MVGKNILLIYQSIPFLLRGLVVTIGLVGGALGLGFILGIPMAIG
                     QVYGNKYISGLISIYVWFFRGLPVLVLLFLFYFGLFILFNLNLSEFTSAVLVLGLISA
                     AYQSQIFRGSIKSVGEGQMLAAISLGIKKYDAILSIILPQALRISIPGWSNEYAILLK
                     DSAIAYAIGVMEVLTRAQFVATRTYKPLPIFFISAIIFIILTYGGVKLLALLEKKVRI
                     PGYGEER"
     gene            1388..2170
                     /locus_tag="PKJ95_04300"
     CDS             1388..2170
                     /locus_tag="PKJ95_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNR65498.1"
                     /translation="MNLEKADHDNGVVLRVEDIHKTYGKNEILKGVSFHIKKGETIVI
                     IGASGSGKSTLLRCINQLTIPEKGRIWLEGEEVTHHMKEISHFRQRIGMVFQDFNLFD
                     HLCAVRNVEIGLIKVKKMDKKQAREIAMEELRRVGLERQANQYPAQLSGGQKQRVSIA
                     RALAMNPTVMLFDEPTSALDPELIGEVLEVMRDLAMGGMTMVVVTHEMGFARSVANEI
                     IFIEGGKILDQGTPHKLFTNPEHERTRQFICKIDELYGKTEK"
     gene            2174..>2767
                     /locus_tag="PKJ95_04305"
     CDS             2174..>2767
                     /locus_tag="PKJ95_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNR65499.1"
                     /translation="MNTMHFITDILFPPLLEGLGVTLRLIVYSVPFGFIAGILIAVGR
                     VYGNKLISTFCFLYTLFFRGAPLLVQLFILYFALPSIGLYLSSFTAAVIGFILCSAAY
                     HSEYIRGAIQSIKSGQMMAAEALGMSKIQAVSSIILPQALRRAIPGCSNEIIYLVKYS
                     SLAYMVACVELTGAGKVIASRYFEYTLVFSIVGIIYLI"
BASE COUNT          839 a          464 c          602 g          862 t
ORIGIN      
        1 attgccaaag aaatgggatt tgaagtaaag catcagccta ctgcatggga tggtattatt
       61 acagccctac ttgccaaaaa aattgatatg gtctattccg ggatgtccat tacccctgag
      121 agattagaaa aagtcaattt ttctattcct tactgggaaa tagaccaggc cctatgtgtt
      181 agaaaagatt cagatctaaa cgttatcgct gccttatgtg gtaaatatgt aacaggctca
      241 cagcgaggat gtacagctgc agagtggata gaagcgaatc tggtaaatac cggtataatt
      301 cccaaagaga attttaaatt atatgaaaca ttttctctgg cggtacagga tctagttaac
      361 ggaagacttg attcagcaat gatggatgat gttatggtag aagatgccat tagaagtggt
      421 caacctgtaa agattgttgg tataatcaaa actggcgaag cttatggtgt tgcaatcaga
      481 aaagaagata aagaattgga aaaaatgctg aatgagggaa taaagaaatt gttaaaagca
      541 gacatatggg aagaattaaa gattaaatac cttactgatt aataactgaa aaggagtcag
      601 ggaattaacc gttaccatca attccttgac tcctgaaatt tttgatctgt ggcttcattt
      661 tgtgcgtatc tttataatta tattaaccaa atgaggataa aaggaagcat ctaatggttg
      721 gtaaaaatat cttgttaatt tatcagtcta ttccttttct tttacgagga ttagtggtaa
      781 ctattggttt ggttggcggc gcattgggat taggtttcat tttgggtata cctatggcta
      841 tagggcaggt atatgggaat aaatatatat ccggtttgat ttcaatctat gtctggtttt
      901 tcagaggatt accggtttta gtccttcttt ttctttttta ttttggattg tttattctct
      961 ttaatcttaa tttatccgaa tttacttcag cagtgctggt attaggattg ataagtgctg
     1021 cctatcagtc tcagatcttt agaggcagta ttaaatcagt tggtgaagga cagatgctgg
     1081 cagcaatatc tttaggtatc aaaaaatatg atgcaatatt aagtatcata ttgccacagg
     1141 ctttacgcat ttccataccc ggttggtcta atgaatatgc cattttactt aaagattctg
     1201 caatagccta tgctataggg gtgatggaag tgttaaccag ggcccaattt gttgccacac
     1261 gaacatataa accattaccg atttttttca taagtgctat catcttcatc attttaacct
     1321 atggtggagt aaaattactc gctcttttag agaaaaaagt taggattcct ggctatggag
     1381 aggagagatg aatttggaaa aagcagacca tgataatgga gttgttctac gtgtagagga
     1441 tatacataaa acttatggga agaatgagat cttaaaagga gtatctttcc atatcaaaaa
     1501 aggtgaaaca atagtcatca tcggtgcctc aggcagcgga aagagtaccc tcctccgctg
     1561 tatcaatcag ctgaccattc ccgagaaggg aagaatctgg ttagaaggtg aagaggtcac
     1621 ccatcatatg aaggagataa gccatttccg ccagaggata ggaatggtct ttcaagattt
     1681 taacctcttt gaccacctct gtgcggtaag aaatgttgag ataggcctca ttaaagttaa
     1741 aaaaatggac aagaagcagg ccagagaaat tgccatggaa gagttaagac gtgtgggatt
     1801 ggaaaggcag gccaatcaat acccggcaca actatcaggt ggacagaagc agagagtctc
     1861 catcgccaga gccctggcca tgaatcccac cgttatgcta tttgatgagc caacatcagc
     1921 tcttgaccct gaacttattg gagaagtatt agaggtaatg agggatttag ccatgggagg
     1981 aatgaccatg gtggtggtaa cccatgagat gggttttgcc cgttctgttg ccaatgaaat
     2041 aatattcatt gaaggcggca agatacttga tcagggtacc ccccataaac tttttactaa
     2101 cccggaacat gaacggacca ggcaatttat ttgtaagatt gatgagcttt acggaaagac
     2161 ggaaaaataa aaaatgaata ccatgcactt tattacagat atactttttc ctcctttgtt
     2221 agaaggatta ggggttactc ttagattgat tgtttattcc gtaccatttg gttttatcgc
     2281 cggtattcta atagcagtgg gaagggttta tggaaacaag ttaatttcca ctttttgttt
     2341 tttatatact ttatttttca gaggagcacc tctattggtt caacttttta ttctttattt
     2401 tgcacttccc tctatcggat tatacctctc ttccttcaca gcggctgtta taggctttat
     2461 actttgtagt gccgcttacc attcagaata tatcagggga gctatccaat ctataaagag
     2521 cggtcagatg atggctgctg aagctctggg tatgtcaaaa atacaggcag tttcatcaat
     2581 tattcttccc caggctttaa gaagggcaat acccggttgt tccaatgaga taatttatct
     2641 ggtcaaatat tcttcgctgg cctatatggt tgcctgcgta gagttaaccg gtgccgggaa
     2701 agtcattgct tcccgttatt ttgaatatac cttagtattt tcaatcgtgg ggataatata
     2761 tttaata
//
LOCUS       DAOQWT010000180         2750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_39996_length_2750_cov_3.625232, whole genome shotgun sequence.
ACCESSION   DAOQWT010000180 DAOQWT010000000
VERSION     DAOQWT010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39996_length_2750_cov_3.625232"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PKJ95_04310"
     CDS             <1..244
                     /locus_tag="PKJ95_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNR65500.1"
                     /translation="ALIAIFVPTSFMDSTVIASALSRVKQEISIPIFANFMAGRLVKE
                     AIEVLESNGIPNYNTGERCAKVIQKIYQYTLRLKNK"
     gene            334..1014
                     /locus_tag="PKJ95_04315"
     CDS             334..1014
                     /locus_tag="PKJ95_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNR65501.1"
                     /translation="MHRLIENAKLEQRSLLEPEAKELLQEYGIYVPAFQLIKDEEEIK
                     KLTESIGYPLVMKIVSPDIIHKSEAGGVKIGIKDEPSARNAYRDILSNTKKYDSKARI
                     YGIITYKMCPISTELIIGMMMDPHFGPVVMFGLGGIFVEVLKDVSFRILPITDRDAME
                     MISEIKGYPILQGVRGENPRDVAAIREVLIKLSALVMENPEIQEIDLNPILVYEKGIQ
                     IVDARMIL"
     gene            1030..>2750
                     /locus_tag="PKJ95_04320"
     CDS             1030..>2750
                     /locus_tag="PKJ95_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HNR65502.1"
                     /translation="MLEQKEHKFDILGRDYDIKNIGKVTGEEKYSCDINIPGQLHAVI
                     LRSPYAHAEIKEVDYTEAEKMGAICIGPQDVPDVIYNERIVSIPAKTYRDRTVFPKDK
                     VRHVGEAIAACAAETEGKAFEALKKIKVIWGKKWEPLVTLDDAMSPDAPAIYDYVYLG
                     EERVKISNNLACDRNIDVGDIEEGFKEADIIADETFDVQRVYHMQLETKSALCQPEAD
                     GGITLWTTTQGIHNVRILLGQIFSIPLSRINVKRITLGGSFGSSIQMNSVTPICVALA
                     LKARKPVKLVTTREEDMYDHSSYPLKTRLKVGVKKDGTLTAAHCQVWLEIGGHNIQAY
                     PYLGCVAGWFASLYRWKNLRYEGKAYYSNKGPACARRGYGSPQVNFPVENMIDILAEK
                     LGMDPIEFRRKNYVGKGEEFWGQGPTVRSLIRSCGVEEMLDRGAELIEWDKKSELKNK
                     NGKIKRGIGVARGFHTSGTGGPKAGEVIDYSGATVKINEDGSVDVITALMDHGGGTWE
                     AGAKVAAEVLKVPFEKVSIDNGVDTRTTVFDVNTHATRGVYCGCGAIKYVAEKVKEML
                     LNYAATL"
BASE COUNT          911 a          444 c          655 g          740 t
ORIGIN      
        1 tgcccttatt gctatctttg ttccaacttc ttttatggat tcaaccgtaa ttgcttctgc
       61 tttaagcaga gtgaaacagg aaataagtat acctatattt gctaatttta tggctggaag
      121 gttggtgaaa gaagcaattg aagtattgga aagtaacggt atccccaact ataataccgg
      181 agaaagatgt gccaaagtga ttcagaaaat ataccagtac accctgagat taaagaataa
      241 atgaaaagaa aaagattaga gtaaacaaga atagataaaa cagaactaat atcaataata
      301 atagtcgatt attgatagat acaaggagca aaaatgcaca ggttaataga aaatgctaaa
      361 ttagagcaaa ggtctttact ggaacctgaa gccaaagaat tattacaaga atatggtata
      421 tatgttcctg cctttcaact gattaaagat gaagaagaga taaaaaagtt gactgagtca
      481 attggctatc cacttgttat gaaaattgtc tcacccgata ttattcataa atcagaggca
      541 ggaggagtga aaattggaat aaaagatgag ccctctgcca gaaatgccta tcgggacatt
      601 ctatccaata ccaaaaaata tgatagcaag gcacgcatat atggtataat tacatataag
      661 atgtgtccca tatccacaga actaattatt gggatgatga tggatcctca ctttggtccg
      721 gtagttatgt ttgggcttgg tggaatcttt gtagaggtat taaaagatgt ctccttccgt
      781 attcttccca taacagacag agatgccatg gagatgataa gtgagatcaa gggatatcct
      841 attttacagg gggtgagagg ggaaaatccc agggatgtag ctgctattag ggaagtttta
      901 atcaagctat ctgcactggt catggaaaac cccgagatac aggaaattga tttgaacccc
      961 atcttagtat atgaaaaggg catacagatt gtggatgccc gaatgatatt ataaacggag
     1021 gtgttattat tgttagaaca gaaagaacat aaattcgata tactgggcag agattatgat
     1081 attaaaaata ttggtaaggt aacaggtgag gaaaaatatt cctgtgatat caatattccc
     1141 gggcagcttc atgctgtcat cctgcgtagt ccctatgccc atgctgaaat taaagaagtg
     1201 gattatacag aggcagaaaa aatgggagct atttgtattg gtccacagga tgtacctgat
     1261 gttatatata acgaacgaat agttagcatt cctgccaaaa catatagaga tagaacagtt
     1321 tttcccaaag acaaggtacg tcatgtcggt gaagcgattg ccgcttgtgc tgcagaaaca
     1381 gagggaaaag cctttgaagc acttaaaaaa attaaagtaa tatggggtaa gaagtgggag
     1441 ccactggtaa cgcttgatga tgccatgtct cctgatgccc ctgctatcta tgattatgtt
     1501 tatttagggg aagagagagt taagataagc aacaatcttg cctgtgaccg taatattgat
     1561 gtaggtgata ttgaagaagg ttttaaagag gctgatatca ttgcagatga aacctttgat
     1621 gtgcaaaggg tttatcatat gcaattagag acaaaatcag cactatgtca gccggaagct
     1681 gatgggggca ttaccctttg gacaaccact caggggattc ataatgtccg tattcttttg
     1741 ggacagatct tctctatccc tttaagccgt attaatgtga aacgaattac cttaggtgga
     1801 tcatttggtt ccagtattca aatgaattcg gttactccca tctgtgttgc cctggcttta
     1861 aaagcaagaa aaccggtcaa attggttact acacgggaag aggatatgta tgaccattcc
     1921 agttatcctt tgaaaacacg tttaaaagtt ggtgtaaaaa aagatggtac cctaactgct
     1981 gcgcattgtc aggtctggct ggaaattgga ggacacaata ttcaggctta tccctacctg
     2041 ggttgcgtag ctggctggtt tgcctctctc tacagatgga agaatcttag atatgaagga
     2101 aaggcttact atagcaacaa aggtcctgcc tgtgccagaa gaggctatgg cagtcctcag
     2161 gttaattttc cggtagagaa tatgatagat atactggcag agaagttagg tatggacccc
     2221 atagaattcc gtcgaaagaa ttatgtaggc aaaggggagg aattctgggg gcaaggaccc
     2281 acagtgaggt ctctcattag aagctgtgga gtcgaagaga tgcttgacag gggagcggaa
     2341 ctgattgaat gggataaaaa atctgagtta aaaaataaaa atggtaagat taaaagggga
     2401 atcggagtag cgagaggatt ccatacctca gggaccggtg gtcccaaagc gggtgaggta
     2461 atagactatt ctggagcaac tgtaaagatc aatgaagatg gttcagtcga tgtcattacc
     2521 gcccttatgg accatggggg aggtacctgg gaggcaggag ctaaggtagc agcagaagtt
     2581 ctaaaggtac cctttgagaa agttagcatt gacaatggtg tggatacccg aaccacagtc
     2641 tttgatgtga atacacatgc aactagaggt gtttattgtg gatgtggagc tattaaatat
     2701 gttgcagaaa aagttaaaga aatgttgcta aattacgcag ctaccctctt
//
LOCUS       DAOQWT010000181         2732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_40391_length_2732_cov_6.166604, whole genome shotgun sequence.
ACCESSION   DAOQWT010000181 DAOQWT010000000
VERSION     DAOQWT010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40391_length_2732_cov_6.166604"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..472
                     /locus_tag="PKJ95_04325"
     CDS             29..472
                     /locus_tag="PKJ95_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65503.1"
                     /translation="MEPVKLISDEQRHAMKLNKGELLTLMEEYKVLEMKEQAIIERKK
                     WIKSKLCGLVKNQGEKKTDKQTSYKSEITRLEALVTTKRNIKFTDKNGLMNYCRQAGF
                     RDLIVTEEDIDKKRFTAKWDNGALPQDISMFIEISYDDSLQVKEW"
     gene            477..764
                     /locus_tag="PKJ95_04330"
     CDS             477..764
                     /locus_tag="PKJ95_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65504.1"
                     /translation="MYKELLQAYTDEYDRKKKEGEEIEELKRKALEAGVEINNHIFTD
                     NLLWNVRHSTIKHMIEDEIEFRNKLREKALKEKEAEQCKQAIKNPEEATEL"
     gene            761..949
                     /locus_tag="PKJ95_04335"
     CDS             761..949
                     /locus_tag="PKJ95_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65505.1"
                     /translation="MRNTEKEIKKACKLAVEEIIFDLSDRRGLRQEWEQIDDDIVEEI
                     KEKWQEIIFNQFKEGLSK"
     gene            946..1431
                     /locus_tag="PKJ95_04340"
     CDS             946..1431
                     /locus_tag="PKJ95_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65506.1"
                     /translation="MILAFDPGDHTGYCVMDDHRIIETGEIRYPQKKSIKHLEELIYM
                     NNIGTIYEKYRPIAVIIEENFMGRFIAVVKALTKKLTLIRIAIKLRYEDALIITPTPS
                     EWKALLDIPQKTKFDQRPEKEKVLTRIKQLIPGYSIDNEHITDAVAMCLAYEGRVRVK
                     L"
     gene            1428..1628
                     /locus_tag="PKJ95_04345"
     CDS             1428..1628
                     /locus_tag="PKJ95_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65507.1"
                     /translation="MKPLEPCPICGQPTHIIKEGNYEWETCNRQCIKKIHGFKLAVTL
                     FDLIKQWNIRRYRISCTVSKGN"
     gene            1677..1868
                     /locus_tag="PKJ95_04350"
     CDS             1677..1868
                     /locus_tag="PKJ95_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65508.1"
                     /translation="MEYFEQLARNIERLDPDEIRQIINLCKFHLASRGIDPERRLKLE
                     KAVLPEPKPLAEVLQFRGG"
     gene            1879..2283
                     /locus_tag="PKJ95_04355"
     CDS             1879..2283
                     /locus_tag="PKJ95_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65509.1"
                     /translation="MKERIKEYMGINQPSPTPKTYTFQFKGGGKARFGKPYVAKLICN
                     HYLKERFKREYLPIPIQKITFGETKEIISYQAEGKFTICDKEIVVIYYGAWEYKDRKR
                     EYKTCSNGELIPVNKDKLYSYFKGDLQWKDLF"
     gene            complement(2311..2448)
                     /locus_tag="PKJ95_04360"
     CDS             complement(2311..2448)
                     /locus_tag="PKJ95_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65510.1"
                     /translation="MPKFSKFSKMERGRTAFAGLPPPVLMELRFDVSENPLKFVYYYT
                     I"
BASE COUNT         1044 a          430 c          590 g          668 t
ORIGIN      
        1 atcaagaagg caacgaggag gtaatattat ggaaccggtt aaattaatat cagatgaaca
       61 gaggcatgcc atgaaactga ataaaggcga actccttaca ctcatggaag aatataaagt
      121 tctggagatg aaagaacagg ctattataga aagaaagaaa tggattaaat cgaagctctg
      181 tggattagtg aaaaatcagg gagagaaaaa aactgacaaa caaaccagtt ataaaagtga
      241 aatcaccaga cttgaagccc tggttactac taagaggaat attaaattca cagataaaaa
      301 cggactgatg aattactgta gacaggccgg gttcagggat ttaattgtaa cagaggaaga
      361 tatagataaa aagagattta ctgcaaaatg ggataacggg gcattaccgc aggacatatc
      421 tatgtttatt gaaataagct atgatgatag tttacaagtg aaggagtggt aaggagatgt
      481 ataaagaatt actacaggca tatactgacg agtacgaccg gaagaaaaag gaaggagaag
      541 agatagaaga attaaagcgg aaagcacttg aagcaggtgt agaaataaac aatcatatat
      601 ttacagataa cctgctatgg aatgtgagac actctacaat aaaacatatg atcgaagatg
      661 aaattgagtt tagaaataaa ttaagggaaa aagcactgaa agaaaaagaa gcagagcagt
      721 gtaagcaagc gataaaaaac ccggaggagg ctacggagtt atgagaaata cagaaaaaga
      781 aattaaaaaa gcatgtaagt tagcagtaga agaaataata tttgatttat ctgacagaag
      841 agggttaagg caagagtggg aacagataga cgatgatata gtagaagaga taaaagagaa
      901 atggcaagaa ataatattta atcagtttaa ggaaggatta agcaaatgat tttagccttt
      961 gacccaggag atcacaccgg atactgtgtt atggatgacc acaggattat agaaacaggg
     1021 gaaatccggt atcctcagaa gaaatctata aaacacttgg aagaacttat ttatatgaat
     1081 aatatcggaa ccatctatga gaagtatcgg ccaatagcag ttatcataga agaaaacttc
     1141 atgggcagat ttatagcagt ggtgaaagct ctgacaaaga agcttacttt aatcagaatt
     1201 gctattaagc taagatatga agatgcactt attattacac ctaccccttc agaatggaaa
     1261 gccctgttag atatacccca gaaaaccaag tttgaccaga ggccggagaa agagaaggta
     1321 ttgacccgga taaaacagtt aataccagga tatagcatag acaatgaaca catcaccgac
     1381 gctgtagcta tgtgccttgc atatgaaggg agagtgaggg ttaagctatg aaaccacttg
     1441 aaccctgtcc catatgcgga cagccgacac acatcataaa agaagggaac tatgaatggg
     1501 aaacctgtaa caggcagtgt ataaagaaga tacacggatt taaacttgct gttaccttat
     1561 tcgacctgat aaaacaatgg aatatcagaa gataccgaat ttcctgcacg gttagtaaag
     1621 gaaattgaac ggttagggaa aatctctacg attagatcat aaaaaggagt gaagatatgg
     1681 agtattttga acaattagca agaaatattg aacggttaga tcctgatgaa atcaggcaga
     1741 taataaacct ctgtaaattc cacctggcaa gcagaggcat agacccggag agaaggctta
     1801 aattagaaaa ggcggtgctg ccggagccta aacctttggc agaagtcctg cagttcaggg
     1861 ggggatgaag acgaattctt gaaagaaaga ataaaagaat atatgggaat taatcagcca
     1921 tcaccaacgc caaagaccta taccttccaa tttaaaggcg gtgggaaagc ccgatttgga
     1981 aagccatatg tggcaaagtt aatatgtaat cattatttaa aagaaagatt taaaagagaa
     2041 tacttaccta taccaataca aaaaattaca tttggagaga ctaaagaaat aatatcatac
     2101 caagcagaag gaaaatttac catatgtgat aaagaaatag tggtaattta ttatggcgca
     2161 tgggagtata aagatagaaa aagagaatat aaaacctgct ctaatggaga attaatcccg
     2221 gtaaataaag acaaattata ctcatatttc aaaggagatc ttcaatggaa agatttattt
     2281 taatagggat tatgttgatt atggactctg ttatatggta taataatata cgaatttcag
     2341 aggattttca gatacatcaa atctcaactc catgagtaca gggggaggca gcccggcaaa
     2401 ggcagtcctc cctctctcca ttttggaaaa cttggaaaac ttcggcaaaa aatctctccc
     2461 ctgacggtac taggcttact ttttctctct ttttttcttt tatacacccc tcccccacag
     2521 gcccccggct gggcgttttg ggcgttttgg gcgttccctg gctgacccca ttccgtcgcg
     2581 acagacgttt tttattaatt ttcttaaatt tacaggagtt ttctaaagtg aaccttttga
     2641 actgtcaata gttttaaacc ccatatgtta cagggacttt acccacctga catataacat
     2701 tatgtaacaa ccttgaaact attgagtttt tc
//
LOCUS       DAOQWT010000182         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_40532_length_2726_cov_6.556720, whole genome shotgun sequence.
ACCESSION   DAOQWT010000182 DAOQWT010000000
VERSION     DAOQWT010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40532_length_2726_cov_6.556720"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PKJ95_04365"
     CDS             <1..527
                     /locus_tag="PKJ95_04365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNR65511.1"
                     /translation="TGSWIIWCAEQQHGHIRDWAVDRGFYPKQMLCWVKANPLPNFRL
                     CGYRQATEIMIWIAKRPIGRKNSNFIFSHQNEMTNVFYAPSVSGKEKTPHRNQKPLSI
                     CQKIIRTHCKPGGIVLDAFSGSGSIALAAYSTGRNFIAIEKKERWNKVAEKRIREYQV
                     VKEEKTDKFEQLKF"
     gene            542..892
                     /locus_tag="PKJ95_04370"
     CDS             542..892
                     /locus_tag="PKJ95_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65512.1"
                     /translation="MGNLKAIVNSITKRKEELQRLLCQLIHDITPEVDVETMDIPLSK
                     EEIHLQTIIGADRVPVISLTIYIPREGVIESLDTKELRVDMLINIEKTCPEVYRETLE
                     KALDLNRREKSKWN"
     gene            complement(1113..1403)
                     /locus_tag="PKJ95_04375"
     CDS             complement(1113..1403)
                     /locus_tag="PKJ95_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65513.1"
                     /translation="MKISFENFFSSMEQVKTVVKFIFKWYPMVKQILQVIEGNVQSEL
                     LEKIIYWMNEAEKLGVSGPEKKDFVIKKAEIPEDKTGVVDTLVDITNLFYKK"
     gene            complement(1408..1992)
                     /locus_tag="PKJ95_04380"
     CDS             complement(1408..1992)
                     /locus_tag="PKJ95_04380"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HNR65514.1"
                     /translation="MAIVITQKESPLFFQDTINGKIVYSGTYSGMVSKLEELAKIYDA
                     PVGKKVKEVLEICEHERLEGGSDFKETEGPNSGPQVRKYLAYVGVTVPAPWCAALVCW
                     VMGQAKINFIKTADTWAMETYAKNKNILTDTPSPGAIFLQINPETGRPVHTGFVKEVD
                     PTDKRYLLTIEGNAGDSIKHCNDKKVTACKYIRW"
     gene            complement(2147..2365)
                     /locus_tag="PKJ95_04385"
     CDS             complement(2147..2365)
                     /locus_tag="PKJ95_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65515.1"
                     /translation="MWSWGIKKSKDFQPEEIMKKHLSEENLKKLQDMDELYIGVKNSI
                     ENSINLSTSFARDYDPEESPRIDSWDRA"
     gene            complement(2366..>2726)
                     /locus_tag="PKJ95_04390"
     CDS             complement(2366..>2726)
                     /locus_tag="PKJ95_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65516.1"
                     /translation="KIYKKGQNMLHSIVAQVQPDISTEAKFLQYAVFLLITAVGILFY
                     LCRDSWLSRINEAVTYAKQVIDIQQKNYELLVGLKSSIDASNKATENLITVLSNTQNA
                     DLKKQIKDLEDQKKGVS"
BASE COUNT          845 a          525 c          462 g          894 t
ORIGIN      
        1 ctactggatc atggattatc tggtgtgctg aacagcaaca tggacacatc agagattggg
       61 ctgtcgatag aggcttttac ccaaaacaaa tgttatgctg ggtaaaagct aatccccttc
      121 caaattttcg cctctgtgga tacagacaag ctacagaaat aatgatatgg atagcaaaaa
      181 gacctatagg gagaaaaaat tctaatttta ttttctccca tcaaaacgaa atgaccaatg
      241 tattttatgc cccttctgta tccgggaaag agaaaacacc gcatagaaat caaaagcccc
      301 tgtcaatttg ccagaagata ataaggactc attgtaaacc cggtggtata gtccttgatg
      361 cctttagtgg atcgggaagt atagcattag ccgcttattc tactggtagg aatttcatag
      421 caatagagaa gaaagaaagg tggaataaag tagcagagaa gaggataaga gaataccagg
      481 tcgtaaagga agaaaagacg gataagtttg aacaattaaa attttaagag gaggtatcat
      541 catgggtaac ttaaaagcta tagtaaacag tattactaaa agaaaagagg agttacagag
      601 gttactctgc cagttaatcc acgatataac gccggaggtt gatgtagaga ctatggatat
      661 acctctgtcg aaagaagaga tacatctaca gacaattata ggtgctgata gagtgcctgt
      721 aatatccctg actatttaca tacccagaga aggagtaatt gagtctttgg atacaaaaga
      781 attaagggtt gatatgctta ttaacataga aaaaacttgc cctgaagtat acagggaaac
      841 attggaaaaa gctttagact tgaaccgaag agaaaagagc aaatggaatt aaaagtaaaa
      901 ctcccctgat ggcctatcgg gggagtatgc aagccttttg taccacaatg aatctctact
      961 cttgtgttct taaaaatcaa gataattttt cctctgacta cgaaatgtcg ttttcagagc
     1021 aaggaggagg ttcaccacct ttcgtttata aaataatatg tgaggaaatc catcctcaca
     1081 tatatatttc tatcctgatg taattattcc tgttattttt tataaaaaag atttgtgatg
     1141 tcaaccagag tatctacaac accagtttta tcttcaggta tctctgcttt ttttatcacg
     1201 aaatctttct tttctgggcc ggatacaccg agtttctctg cttcattcat ccagtaaata
     1261 attttttcca gtaattcaga ttgcacatta ccctcaataa cctgaaggat ttgtttaacc
     1321 ataggatacc acttgaatat gaattttact acagttttaa cctgctccat ggaactgaag
     1381 aaattttcaa atgatatttt cataaaatca ccaccttata tatttacagg ctgtaacttt
     1441 tttatcatta cagtgtttaa tagaatctcc agcattacct tcaatagtaa gaaggtatct
     1501 tttgtcggta ggatctactt ccttaacgaa acctgtatgg acaggtcttc cagtttcagg
     1561 gttgatttgt aaaaaaatag ctccaggtga aggagtatca gttaatatat ttttattttt
     1621 tgcataagtt tccattgccc atgtatcagc agttttaatg aagtttattt ttgcctgtcc
     1681 catgacccaa catacaaggg ctgcacacca gggagcaggt acagttacgc ctacatatgc
     1741 cagatatttc ctgacctgcg gcccggagtt tggcccttca gtttccttaa agtctgaacc
     1801 cccttcaagt cgttcatgct cacagatttc caggacttct tttactttct tccctactgg
     1861 agcatcataa atttttgcca attcttctaa ttttgaaacc attcctgaat aagttcctga
     1921 ataaactatc tttccattaa tagtatcttg gaagaataaa ggactttctt tttgagttat
     1981 tacgattgcc atgtttatat ctccttatgc ctatcacaat gtttctcgct acattcttta
     2041 cataactctc cagaaacaaa actatttaca ggacacttat cacgattgcc acactcctca
     2101 atagcttcgt tcacttctcc aaatatgtca agaacaatta cccgccctac gccctatccc
     2161 acgaatcaat tcgtgggctt tcctcaggat cgtaatctct tgcgaaactt gttgataagt
     2221 ttatactatt ttcaatacta ttttttaccc ctatgtatag ttcatccata tcctgtaatt
     2281 tctttaaatt ttcttccgat aaatgctttt tcattatttc ttctggttga aaatccttac
     2341 tcttctttat tccccatgac cacatctaac tcaccccctt tttttgatct tcaaggtctt
     2401 tgatctgttt ttttaagtct gcattttgag tattacttaa aactgtgata agattttcag
     2461 tggctttatt tgaagcgtct attgaggact tcaaccctac gagtaattca taattcttct
     2521 gctgaatatc gataacctgt tttgcataag ttactgcctc gttaatcctt gaaagccatg
     2581 aatctctgca taaataaaat aaaattccta ctgcggttat taaaaggaaa actgcatatt
     2641 gaaggaattt agcttctgtt gaaatgtctg gttgaacctg tgcgactatt gaatgtaaca
     2701 tattttgtcc tttcttataa atttta
//
LOCUS       DAOQWT010000183         2718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_40735_length_2718_cov_3.082989, whole genome shotgun sequence.
ACCESSION   DAOQWT010000183 DAOQWT010000000
VERSION     DAOQWT010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40735_length_2718_cov_3.082989"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1273
                     /gene="ggt"
                     /locus_tag="PKJ95_04395"
     CDS             <1..>1273
                     /gene="ggt"
                     /locus_tag="PKJ95_04395"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNR65517.1"
                     /translation="TGEIIILDYRERAPAASTKDMFASEQAKEEKWTQLSGLASGVPG
                     TLLGLKTALDEYGTMTLKEVMAPAIEYMENGFEITDTFRDMIKDNYDKLVDWNDPFAI
                     AYLKDGVPLETGDILIQKDLAKTYREILDKGIEHFYGGELGQKIVDAVQAAGGILTLQ
                     DLKDYKVFRHKPVSGSYRGYDIYSMPPPSSGGTHLIQILNIMENFDIAEMNYLGPTHI
                     SVLSEAMKMAFADRAKFMADPAFAKDIPLEGLTSKEYAKSLAELIDVSNPKLEIPAGE
                     PGLFEHQSTSHLSVIDAEGNAVALSQTINYFFGSGLIVPGVGIIMNNEMDDFSTSPTS
                     PNAPEPGKTPLSSMSPTIMEKDGEVFMVLGSPGATRIFTAMAQIISNVIDFNMGMDEA
                     IEAPRIHAYSSAGKAMPIFVEDRIPLLTIEA"
     assembly_gap    1274..1283
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1529..>2718
                     /locus_tag="PKJ95_04400"
     CDS             1529..>2718
                     /locus_tag="PKJ95_04400"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNR65518.1"
                     /translation="MLSIELLYFVVMVGVFAVSCFRFKLPVGVAMALSAISGCLVAGF
                     GIPIRHLVEGGLNYLNTILVISTAMIFMKVVQYNGMMDTVTRWLIEKYYSKPALLIIG
                     LSFIVVFPGMITGSSTAAVLTGGVLVSPVLIYIGFPKEITAAVVAMAAVFGEAAPPVN
                     VNALIIGGGIDLPYIGFTLPLLLLTIPLIIFTSLFLGKKYLKVFNYEKIKDDLPTSYL
                     SDFGIWLFLPIIILFILMIRETFLPKLFPTLGMPIYFLIAALVGLFSGKKFNFLKIAQ
                     EAVHDALPVLGILVGVGMFIQVMTLTGVRGYIVINCLSLPAYLLYLGIAVSIPAFGAV
                     SAYGSASVLGVPFALALLESNPVITVAALSQLSALGDLVPPTALAGIFAAQVVGEKNY
                     FKVL"
BASE COUNT          755 a          494 c          608 g          851 t
ORIGIN      
        1 ggaccggaga gattataatc ttagactacc gggaacgtgc accggcggct tcaactaagg
       61 atatgtttgc ctctgaacag gccaaagaag agaaatggac tcaattgagt ggattggcaa
      121 gcggtgttcc cggaaccttg cttggactta aaactgctct cgacgaatat ggcactatga
      181 ccttaaaaga ggttatggct cctgctatag agtatatgga aaatgggttt gaaataacag
      241 atacttttag agatatgatt aaggataatt atgataaatt agttgactgg aatgatcctt
      301 ttgctattgc ctatcttaag gatggagttc ctctggaaac aggagatata ttaatacaaa
      361 aagatctggc aaagacttat cgggagatac ttgataaagg aattgagcat ttttatggtg
      421 gtgaattggg tcagaaaata gttgatgctg ttcaagctgc aggtggcata ctcactcttc
      481 aagatttaaa agattataaa gtattcagac ataaaccagt tagtggcagc tatcggggat
      541 atgatatcta ttccatgcct ccccccagct ctggtggcac tcatcttatt caaatcctga
      601 atataatgga aaactttgat atcgcggaaa tgaattatct gggaccgact catatctcag
      661 ttttgtcaga agctatgaaa atggcttttg ccgatagagc aaaatttatg gctgacccgg
      721 catttgctaa agatattcct cttgaaggac tcacttcaaa agaatacgca aaatctctgg
      781 ctgaactgat tgatgtatct aacccaaaat tagaaattcc tgccggtgaa ccaggcctat
      841 ttgaacatca aagtaccagt catctttcgg tcattgatgc cgaaggtaat gcagtggctt
      901 tatcccagac tatcaattat ttcttcggtt caggactgat tgttcccggg gtggggatta
      961 ttatgaataa tgaaatggat gatttttcta ccagtccaac ttctcccaat gctcctgaac
     1021 caggtaaaac acctctctcc agtatgtcgc ccacaataat ggaaaaagat ggcgaggtat
     1081 ttatggttct aggctctccg ggtgcaacca gaatcttcac agctatggca cagataatct
     1141 ctaatgttat tgattttaat atgggaatgg atgaagctat tgaagcgccc cgtattcatg
     1201 cctattcttc ggcaggaaaa gcgatgccta tttttgtaga agaccgcata cctctattaa
     1261 ctattgaagc attnnnnnnn nnngcattaa gattactggg taatcaagta gatgtgaagg
     1321 gaacccatga tctttatttt ggtggagctc agggcatttt attgaaaaat ggtgtactct
     1381 ttggtggtgg tgattcgcgt cgtgatggaa tagctatcgg ctactaaaaa agctgctaag
     1441 ggacggtcct ttgacatctt ttagacaaga taaaaagatg tcaaaggacc gtccctgaac
     1501 agatttgaga gaagaaagga ggggtatatt gcttagtatt gaattgcttt attttgtggt
     1561 aatggtaggt gtgtttgcag taagctgttt caggtttaaa ctgccggtag gggtagccat
     1621 ggcgctatct gctatttccg gttgcctggt agcaggattt ggtatcccca tcagacatct
     1681 ggtggaagga gggctcaatt atcttaacac aattctggta atttcaactg caatgatttt
     1741 tatgaaggtt gtacagtaca atggcatgat ggatactgtc accagatggt taattgaaaa
     1801 atattattcc aaaccggctt tattaattat tggcttatcc tttattgtgg tatttcccgg
     1861 aatgattacc ggctcttcta cagcagctgt tttaactggt ggtgttctgg tttcaccggt
     1921 attgatatat attggttttc caaaagaaat aaccgccgca gtagtggcaa tggctgcggt
     1981 ttttggagag gcagctccac cggtcaatgt taatgctttg ataataggag gaggaattga
     2041 cctaccctat atcgggttta ccctgcccct gttattactt accattccct tgattatatt
     2101 caccagcctt tttttaggga aaaaatatct taaagtattt aattatgaga agataaaaga
     2161 tgatcttccc acttcttatc tttcagattt tgggatatgg ttattcttac ccattataat
     2221 cctttttatc cttatgataa gggagacctt cttgcccaaa ctgttcccca ccctgggaat
     2281 gcccatatac tttttgattg cagcactggt gggtctattt tctggcaaaa aatttaactt
     2341 tttaaaaata gcacaggaag cagttcatga cgccttaccg gttttgggta ttttagttgg
     2401 agtagggatg tttatacagg ttatgaccct gaccggagta aggggttata ttgtcattaa
     2461 ttgtttaagc ctgcccgcct atttacttta tctcgggata gctgttagta taccagcttt
     2521 tggagcagta tctgcttatg gttcagcatc ggtcttaggt gtgccatttg ccctggcttt
     2581 actggagagc aatccggtaa ttacagtggc agctttatct caattatccg ctttaggtga
     2641 cctggtgccg cccactgctt tagctggaat atttgcagca caggtagtag gagagaagaa
     2701 ttactttaaa gtactaaa
//
LOCUS       DAOQWT010000184         2706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_41030_length_2706_cov_3.738966, whole genome shotgun sequence.
ACCESSION   DAOQWT010000184 DAOQWT010000000
VERSION     DAOQWT010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41030_length_2706_cov_3.738966"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PKJ95_04405"
     CDS             <1..818
                     /locus_tag="PKJ95_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR65519.1"
                     /translation="ELNHNCIINALRLARPADKLIYRHLNMQELEEQLKKSIDRAKRI
                     LIITDGIFSMRGDYAPLDEISKISQKYEQHFPEGIVLVVDDSHGVGCFGETGRGTEEY
                     TQIDGVDILIGTLGKAFGVNGGYATSSQTVITYLRENAPMYIYSNPITPAEAREAYRA
                     LEILESEEGKGILKHLKEMTKRFEKGLKELGYEIIESDHPIVPLMVRDTRKTKELVDY
                     LIENGVLATGLNFPVVPRGDEEIRFQVNASHTPYDIDTVLEVLKKWKGINQIP"
     gene            1156..>2706
                     /locus_tag="PKJ95_04410"
     CDS             1156..>2706
                     /locus_tag="PKJ95_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNR65520.1"
                     /translation="MSILSFLHFLSFLIYFTMFLFLLWKGIESLSSRVLTAFIGCFAF
                     WSLTSIFFFQPNTTKTTALLIDNIASISWISCHMFYLWFAIIFTEKEKVLKNNIIYFV
                     FFALPLLFIYKNWTGFLINDYIRQPWGWEKIWSDSLWTYSFFLYYPSCSLIALYLIYD
                     FSKKKKMTLKGKQSRIIFYTSLISLTITSFIDILLPLYNVDKFPPVANVAYLILIGGI
                     IYAIVKYRFIPVTPHMAAEQIISTMADSLVLLDKNCNIASVNQAFINLTGYENKELLG
                     KTMELFIEKTILEKMLTDIVSQKKSKKEIEMKLQTKSSSQISALCSCSPVIDDSGELA
                     GIVCIFTEITELEKAKKILLESQEESISLFQNSPLAGLYQDENGIILDINKKFTELFG
                     YSREELKGKNINEGFIFPEERIVLESDSLTQKSLANGHIQYETIRKKKDGTIVPVVIT
                     VSSIIVKDKRKAIVAFYQDISKEKEFLDKLKQSESKYRDLFENIPAAYYQTDKDGNIV
                     MMNPAGIVL"
BASE COUNT          937 a          414 c          504 g          851 t
ORIGIN      
        1 atgaactgaa tcataattgt attatcaatg cccttcgttt agctcgtccc gcagataagc
       61 tcatctatag acatctgaat atgcaggaat tagaagaaca gctaaaaaag agcatcgaca
      121 gggctaagcg tatcctgatc attacagatg gaattttcag tatgagggga gattatgccc
      181 cgcttgatga gatttcaaaa atttcccaaa agtatgaaca acactttccg gagggaattg
      241 tattagtagt agatgattct catggtgtag gttgttttgg ggaaacaggt cgtggaacag
      301 aagaatacac tcaaatcgat ggtgtggata ttctcatagg tactttagga aaagcctttg
      361 gagtaaatgg aggctatgcc acatccagtc agaccgtaat cacctatttg cgagaaaatg
      421 caccaatgta tatctattcc aatcccatta ctcctgctga ggcacgggag gcatatcggg
      481 cactggaaat actggaaagt gaagagggta aaggaattct caaacatcta aaagaaatga
      541 ccaaacgatt tgaaaagggg ctgaaagagc tggggtatga gattattgaa agtgaccacc
      601 ccattgttcc tcttatggtc agagatacca gaaaaaccaa agaattagta gactatctga
      661 ttgaaaatgg tgtgcttgcc actggactta atttcccagt tgttccccgg ggagatgaag
      721 agattcgttt tcaggttaat gccagccata ccccttatga tattgatacc gttctagaag
      781 tgttaaaaaa gtggaagggg attaatcaga ttccttaaat ataaatgaag gcagggatta
      841 tttgcttttt tatattcttt atactaaact gtaaaaggta aactaatgtt cttttaaaat
      901 atctaatttt atttttatat tctataaata taaaggatta aaaagaatat ttgctgttga
      961 aatttaatca ttaaggatat ttaggcataa accagaagaa ctcgtaaaaa gggctattta
     1021 ttactattat agttaaaata ctataagaaa acacaatata accataacaa ttaaaagaaa
     1081 tattaaaaaa taatagcaaa aaagaagaaa ataatagtaa tagtgtttat agaatcatta
     1141 tttaagtgat atgatatgag catattatct tttcttcatt ttttgtcttt tttaatttat
     1201 tttactatgt ttctttttct tctctggaag ggtatagaat ctttatcaag cagggttctt
     1261 actgccttta ttggctgttt tgccttttgg agtcttacct caattttttt ctttcagcca
     1321 aacactacaa aaaccaccgc tctattaata gataacatcg catccattag ctggattagt
     1381 tgccatatgt tttatttatg gtttgccata atcttcactg aaaaggaaaa agtattaaag
     1441 aataatatta tttattttgt attctttgct ctgcccttac tctttattta caagaattgg
     1501 actggttttt taatcaacga ttatataaga cagccctggg ggtgggagaa aatctggtca
     1561 gactctctat ggacatattc ttttttctta tactatccct cttgttctct catagcatta
     1621 tacctaattt acgattttag taaaaagaag aagatgacct taaaaggaaa acaatcccgg
     1681 attatttttt acacctcttt aatatctttg actattacca gttttataga tattttatta
     1741 cccctctaca atgttgataa atttccacct gttgcaaatg ttgcttattt aatactaata
     1801 ggtggaataa tttatgctat tgtaaaatat agatttatac ctgtaacgcc acatatggct
     1861 gccgaacaga taatttctac tatggctgat tctttagttc ttttggataa aaattgcaat
     1921 attgccagtg tgaaccaggc tttcatcaat ttaacaggtt atgagaataa agaactgtta
     1981 ggaaaaacta tggagttatt cattgaaaaa actattctgg aaaaaatgct gaccgatatc
     2041 gtttcccaaa aaaagtccaa gaaagaaatt gagatgaaat tacagactaa aagcagtagc
     2101 cagatctcag ccctttgctc ctgctcacct gtaattgatg atagtggaga attagcggga
     2161 atagtctgca tatttactga gattaccgaa ttagaaaagg caaagaaaat attactggaa
     2221 agtcaggagg agtcaatcag tttatttcaa aatagccctt tggcaggctt atatcaagat
     2281 gaaaacggca ttatcctgga tattaataag aagtttactg aactattcgg atacagcaga
     2341 gaggaattga aagggaaaaa tatcaatgaa gggtttatct tcccggaaga gagaatagtt
     2401 ctggagagtg atagtttaac tcagaagtct ttagccaatg gacatatcca atatgaaaca
     2461 atacgaaaga agaaagacgg aacaatagtg cctgttgtta taacagtatc atccatcata
     2521 gtaaaagata aaagaaaagc tattgttgct ttttatcagg atataagcaa ggaaaaggaa
     2581 tttttggata aattgaaaca gagtgagagt aaatatcgag acctctttga gaatataccg
     2641 gcagcatact atcagaccga caaagatggt aatattgtaa tgatgaatcc agcggggatt
     2701 gtgcta
//
LOCUS       DAOQWT010000185         2703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_41121_length_2703_cov_3.216767, whole genome shotgun sequence.
ACCESSION   DAOQWT010000185 DAOQWT010000000
VERSION     DAOQWT010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41121_length_2703_cov_3.216767"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PKJ95_04415"
     CDS             complement(<1..752)
                     /locus_tag="PKJ95_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65521.1"
                     /translation="MVADKKKILQVKNLKTYFFTEDGVAKAVDGVDFEIYQGETLGLV
                     GESGCGKSVTAFSILRLLDYPGKTVEGQIIFKGADLLKKSDNEMRKIRGKEIAMIFQE
                     PMTSLNPVFTIGYQISEALKIHLKMSNREAKMKSIELLEKVGIPIPKQRFDEYPHQLS
                     GGMRQRAMIAMALSCNPILLIADEPTTSLDVTIQAQILDLMKNLLKQFNSSLIMITHD
                     LGVIAEIADRIAVMYAGKIVEYAETRSIFFSPL"
     gene            complement(752..1687)
                     /locus_tag="PKJ95_04420"
     CDS             complement(752..1687)
                     /locus_tag="PKJ95_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR65522.1"
                     /translation="MYRIIKKLLKNSASLVGLLLLLVFILIAVFAPFIAPPLFPDPYQ
                     IPRMSWDIEPRPPGSPITDDMRFVYESLGKPVIREQAIFGTAQGGYDIFYGMIWGTRT
                     AFRVGIVTVAFSVLIGIIGGSIAAYFGGWIDELLMRLTDIFLSVPFLVAAMVLTTILG
                     RGLDKVMIALIAFGWMGYARVIRGSILCTKEEAYVIAAKALGVKDFRIIAFHLLPNTI
                     FPVLVQASMNIGSMVVTAAALSFLGVGAPLGYADWGQMVSFARNWIIGAPGKAGEYWY
                     AVFFPGIFIVLFVLSWNLVGDTLRDILDPRLRGEL"
     gene            complement(1695..2702)
                     /locus_tag="PKJ95_04425"
     CDS             complement(1695..2702)
                     /locus_tag="PKJ95_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR65523.1"
                     /translation="MLPLILLGVTFVIFSLFSLLSPMEKLATYINNPTVLKNPQAAQK
                     LIQKYHLDDPFPAGYFYWLKSLLKLDFGWSPTANQPVWEAIKQRFPATVELVFYSIIP
                     VILVGIWLGVISSVHHNDILDQTIRVTAIIGWSLPTFIFGLLVLFIFYGILGWLPPGR
                     LSTWAQYIVYTGDSFKHYTHFITFDALLNGRLDIFWDAVKHLIGPIITLSYISWAYLL
                     RITRSSMLDTLRKDYIRTARAKGVEENKVIYQHARKNALIPVVTVAGMMIVEMLGGVV
                     ITETVFTYPGLGFWAASSAQQLDYGSVIGFALLFAFMMVIGNLIVDISYALIDPRVRL
                     E"
BASE COUNT          884 a          613 c          476 g          730 t
ORIGIN      
        1 agaggtgaaa aaaatatgga acgggtttca gcatattcca caattttccc ggcatacatc
       61 accgcaatac ggtcagcaat ctccgcaatt acacctaaat catgtgttat cattatcaat
      121 gagctgttaa actgttttaa aagatttttc atcaaatcta atatctgagc ttgtatagtg
      181 acatctaaag atgtggttgg ttcatcggct atcagcaata tgggattaca ggataaagcc
      241 atagctatca tcgccctctg tctcattcct ccgcttaatt gatgtggata ttcatcaaat
      301 ctctgtttgg gaataggtat tcctaccttt tctaaaagct caatagactt catttttgct
      361 tctctgttgc tcattttaag atgaattttt aatgcttcag aaatctgata acctatagta
      421 aaaaccggat ttaaagaggt cattggctcc tgaaatatca tagctatctc ttttcctctt
      481 atttttcgca tctcattatc acttttctta agcaaatcag cccctttgaa gataatttgt
      541 ccctctaccg ttttcccggg ataatccagt agtcttaaaa tagaaaaagc agtgacactt
      601 ttaccacaac ccgattcgcc aaccaatccc agagtttctc cctgatatat ttcaaaatca
      661 acaccatcca ctgcttttgc cactccatct tccgtaaaaa aataggtttt caaattcttt
      721 acctgtaata ttttcttctt atcagcaact atcacaattc acctctcaga cgcgggtcaa
      781 gtatatctct cagggtatct cctactaagt tccaggaaag aacgaataga acaataaaga
      841 ttcctggaaa aaatactgca taccagtatt ctccagcctt accgggcgca cctatgatcc
      901 agttacgggc aaaactgacc atctgacccc aatctgcata tcccaaaggt gccccaactc
      961 ctaaaaaact taaagctgct gcagtaacta ccatggaacc gatattcata ctggcctgaa
     1021 ccaatactgg gaaaatagta ttgggcagca aatgaaaggc tatgatacga aaatctttca
     1081 ctcccagagc ttttgcagct ataacataag cttcttcttt ggtacataaa atactacccc
     1141 ttattactct ggcatatccc atccagccaa aagcaataag cgctatcatt actttatcaa
     1201 gacctctccc caaaatggta gtgagcacca tagccgcaac taaaaaggga acggagagaa
     1261 aaatatcagt taaacgcatc aacagctcat ctatccagcc cccaaaataa gctgctatcg
     1321 aaccaccgat aattccaatc agcacagaaa aagctactgt aacaattcca acccggaaag
     1381 cagttctggt tccccatatc attccataaa atatgtcgta accaccctgg gcggtgccaa
     1441 aaattgcctg ctctctgatc accggcttgc ccagactttc ataaacaaac ctcatatcat
     1501 cggtaattgg agaacccggc ggccgaggct caatatccca ggacattcgg ggaatttgat
     1561 aaggatccgg aaacagtggt ggggcaatga aaggggcaaa aacagcgata agaataaata
     1621 ctaagagaag taataatcct accagagatg ctgaattttt taacaatttt ttaattattc
     1681 tatacatctg cctgctactc caatctcaca cgaggatcaa ttaaggcata ggaaatatct
     1741 acaatgagat taccaattac catcataaag gcaaaaagaa gggcaaagcc aataactgac
     1801 ccataatcta actgctgggc agaggaggca gcccaaaacc ccaatccggg ataggtaaat
     1861 actgtttcgg taataactac tcctcccagc atttcaacaa tcatcattcc agctactgtt
     1921 accaccggta taagggcatt ttttcgagca tgctgataga taaccttatt ttcttcaacc
     1981 ccttttgccc gggcagtacg gatataatct tttcttaaag tatcgagcat actggaacgg
     2041 gtaattctca ataggtaagc ccaggaaata taagaaaggg taatgatagg tccgattaaa
     2101 tgtttgactg catcccagaa aatatccagg cgcccgttca gaagagcatc aaaagtaatg
     2161 aaatgtgtgt agtgtttaaa actatcccct gtataaacaa tgtattgagc ccaggtactt
     2221 aaccttcccg gaggaagcca gcccaatatg ccataaaaga taaatagcac cagcaatcca
     2281 aagataaaag ttggcagtga ccagcctatt atagccgtaa ctctgatagt ctgatccagt
     2341 atatcattat ggtgaactga agagataaca cccaaccaga ttcccaccag aatcacagga
     2401 ataatggaat agaagaccaa ctctactgtt gccggaaagc gttgcttgat tgcttcccaa
     2461 actggctggt tggcagtagg agaccatccg aagtctaatt tcagcaatga ttttaaccaa
     2521 tagaaataac cggctggaaa ggggtcatca agatgatatt tctgtatcag tttttgagca
     2581 gcctgggggt tttttaagac agtggggtta ttaatataag ttgccagttt ttccatagga
     2641 cttaacaggg aaaaaaggct aaatatgacg aaagttaccc ctaataagat taaaggtaaa
     2701 atc
//
LOCUS       DAOQWT010000186         2703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_41128_length_2703_cov_2.788142, whole genome shotgun sequence.
ACCESSION   DAOQWT010000186 DAOQWT010000000
VERSION     DAOQWT010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41128_length_2703_cov_2.788142"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKJ95_04430"
     CDS             complement(<1..334)
                     /locus_tag="PKJ95_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNR65524.1"
                     /translation="MKKRDLLNELKRRIVFFEYKPRQMLNIKDLSKEFGVSAIPIREA
                     LIRLEEEKLINIIPNQGAYVSDLSFQELKDVFEVRLFFLEIAGKLAIQRAQEDEVSEM
                     KNLVEQIKK"
     gene            complement(517..1329)
                     /locus_tag="PKJ95_04435"
     CDS             complement(517..1329)
                     /locus_tag="PKJ95_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNR65525.1"
                     /translation="MIEKLSLLSPIMQALLAGLFTWGLTALGAGAVFLTREVNRKLLD
                     TMLGFAAGVMIAASYWSLLEPAIAMGEEGRFPGWVPAAIGFLLGGIFLRLIDAVLPHL
                     HIGFSMKEAEGISTTWKRTTLLILAVTIHNIPEGLAVGVAFGAVAAGLPSATISGAIA
                     LTIGIGIQNFPEGMAISMPLRGEGMSARKSFWYGQLSGIVEPIFSIVGAWTVLIARSI
                     LPYALAFAAGAMIFVVVEELIPESQQKGHSDLATMGVMVGFTIMMILDVSLG"
     gene            complement(1417..2316)
                     /locus_tag="PKJ95_04440"
     CDS             complement(1417..2316)
                     /locus_tag="PKJ95_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNR65526.1"
                     /translation="MNKLGNRFKAEMTLLGVVIIWGYTFPVIKNVLNAIPPFTFLTYR
                     FFLAFLSIFLIFRHKLKKFNLQTIYKGFLLGLFLFIGYFGQTVGTQFTTATKTAFITG
                     ISVVLVPVFSFFWIREKLRFNSLVGVLLATLGLFLMNANGELFLINKGDALVFLCAVG
                     FALYIVAVHVYTKEYDYVQLVFIQLVTVFLLFFLMSILLEREALHFSYHLSVFWAIVI
                     TGVFATAFALYLQNRFQQYSSPTKIAIIFSTEPVFGALFSHLILGETTGVHGVIGGIA
                     IFIGMLIAQLEKEEKGEAADLHI"
     gene            complement(2413..>2703)
                     /locus_tag="PKJ95_04445"
     CDS             complement(2413..>2703)
                     /locus_tag="PKJ95_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR65527.1"
                     /translation="YRQIGDFKVDKNGVAISGLLVRHLVLPHNLAGTDQILPWIAQNI
                     SNKTYISLMAQYFPTFRAADYPQLSRPITRKEYQEAKSVLKQCGFENGWLQQ"
BASE COUNT          890 a          574 c          458 g          781 t
ORIGIN      
        1 ccttctttat ctgctctacc aggtttttca tctcgctgac ctcatcctcc tgcgccctct
       61 ggatagcaag tttacccgct atttccagaa aaaataatct tacttcaaag acatccttta
      121 attcttgaaa actcaaatcg ctgacataag cgccctgatt gggaataata ttgattaatt
      181 tttcttcttc cagccgtatc aacgcctccc tgataggaat ggcactcact ccgaactcct
      241 tggaaaggtc ttttatatta agcatctggc gaggcttata ctcaaaaaaa actattcttc
      301 tttttaattc attcaggagg tctctttttt tcatatttga cgatatcttc catatattaa
      361 gaatatattg tttggatatt tatattatat attttaaaaa acaaaaagac aacaccaacc
      421 acaaaaatta ctaatatact gattgacagg tttttaaaaa tagttaccaa aggcagatat
      481 cttttgaagc agtatcttat cttatcttat ctatatctat cctaaagaca catctaaaat
      541 catcataatg gtaaacccta ccattacgcc catagttgcc agatcagaat gacccttctg
      601 ctgtgattcg ggtatcaatt cttccaccac tacaaatatc attgcaccag ctgcaaaagc
      661 aagggcatag ggaagaatgg aacgggcaat taagactgtc caggctccta caattgaaaa
      721 aataggctct acaataccgg acagctggcc ataccagaaa ctctttctag cagacatacc
      781 ctcccctctt agaggcatgg atatggccat tccctccgga aagttctgta ttccgatacc
      841 aatggtcagg gcaatagccc cgctgatagt agctgatgga agaccagcag ctactgctcc
      901 aaaagcaaca cctaccgcta atccttccgg tatattgtgt atggtgactg caaggataag
      961 aagtgtagtc ctcttccagg tagtggatat tccttccgct tctttcatag aaaaaccaat
     1021 atgcaaatgg ggtaatacgg catcaatcaa ccgtagaaaa atcccaccga gcaggaaacc
     1081 gatggcagcc ggtacccagc ccggaaagcg cccttcttca cccatagcaa ttgccggttc
     1141 taataatgac cagtaacttg cagctatcat cacaccggca gcaaaaccca gcatagtatc
     1201 gagaagtttt ctgtttactt ctcgagttaa aaagactgca ccagcaccga gtgctgtcaa
     1261 gccccaggta aatagcccgg caagtagtgc ctgcataatc ggacttaaaa gggataattt
     1321 ctcaatcatc ttgattttcc ttttttacct tgaataataa ttatggcaga gtaatattac
     1381 ttcaacttaa aataattata caattttttg ctctctttaa atatgaagat cggctgcctc
     1441 acccttttct tctttttcta attgtgcaat tagcattcca ataaatatgg ctataccacc
     1501 aattacccca tggacacctg tggtttctcc taaaatcaag tgagaaaaga gagctccaaa
     1561 gaccggttca gtggaaaata taatggcaat tttagtcgga ctggagtatt gttgaaaacg
     1621 attttgtaaa tataaggcaa aagctgtagc aaatacacct gttatcacaa ttgcccaaaa
     1681 cactgacaaa tggtaagaaa aatgcagggc ttctctttcc aataaaatag acataagaaa
     1741 aaacagaaga aaaacagtta ccagttgtat aaaaaccagc tgtacataat catactcctt
     1801 agtgtaaaca tgtacagcaa ctatgtagag ggcaaacccc accgcacaaa gaaaaacaag
     1861 tgcatcccct ttgttaatta gaaagagttc tccattagca ttcatcaaaa atagaccaag
     1921 cgtggcaaga agcactccaa ccaatgaatt gaatcggagt ttttctctaa tccaaaaaaa
     1981 agaaaaaaca gggaccaata ccactgaaat tccggtaata aaagcagttt tggtagcagt
     2041 ggtaaattgc gttcctaccg tttgcccgaa ataaccaata aaaaggaata gacccagtag
     2101 aaatccttta taaatagttt gtagattaaa tttttttaat ttatgccgaa aaatcaaaaa
     2161 gatagacaaa aacgccaaaa aaaagcgata agttaaaaag gtgaaaggag gaattgcgtt
     2221 cagtacgttt ttaataaccg ggaaggtata gccccagata attaccacac ccaatagtgt
     2281 catttctgct ttgaatctgt ttcctaattt gttcatatag ctttatacta ttgctttcct
     2341 taattccttt tattgataat atcaaatcta aattttaata atttttctat actttattgc
     2401 aattattata tctcattgtt gtaaccagcc attttcaaat ccgcattgct tcaggacgga
     2461 ttttgcctcc tgatattctt ttctggtgat aggtcgggaa agctgaggat aatcagctgc
     2521 ccgaaaagta gggaaatatt gagccatcaa gctgatatag gttttgttag atatgttttg
     2581 agctatccag ggtaatattt ggtcggtacc ggcaaggtta tggggtaata ccagatggcg
     2641 taccaacaaa cctgagatag ccacaccatt tttatccact ttgaaatcac ctatctgacg
     2701 ata
//
LOCUS       DAOQWT010000187         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_41176_length_2701_cov_3.371126, whole genome shotgun sequence.
ACCESSION   DAOQWT010000187 DAOQWT010000000
VERSION     DAOQWT010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41176_length_2701_cov_3.371126"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..269
                     /locus_tag="PKJ95_04450"
     CDS             78..269
                     /locus_tag="PKJ95_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65528.1"
                     /translation="MEPFFNSRLQMDFGKIQFNYGGQVNFNNWKEWQENRKEILLRIN
                     DRLSLNWQIPNRFGFTISW"
     gene            899..1990
                     /gene="argC"
                     /locus_tag="PKJ95_04455"
     CDS             899..1990
                     /gene="argC"
                     /locus_tag="PKJ95_04455"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNR65529.1"
                     /translation="MEKIKVSIIGATGYSGKELIRILLKHPRIKLMHLVSASYVGQNI
                     CEVYPEFTNQLDKKLIELDIEKIVRDSDVVFTALPHNISLDIVPELLINKNQMKVIDL
                     SADFRLKDSDNYTKWYKKEHSEKSRVFLKEAVYGLPELYKEDIKKATLVANPGCYPTS
                     AILGMAPLLARRLVEADNIIIDAKSGTSGAGRKLSLGLHFSECNESFKAYKTTMHNHI
                     PEIEQELSLLYNPDSGKDEGEKNKYGKKNKEIIVSFTPHLLPLSRGILSTCYLNLIED
                     CNEQDIVTLYQNFYQDAPFVRIFEPPNLPEIRFAQLTNYCDIGFTIDKRVGKIKVISV
                     IDNLTKGASGQAVQNMNIMFNLPETMGLL"
     gene            2009..>2701
                     /locus_tag="PKJ95_04460"
     CDS             2009..>2701
                     /locus_tag="PKJ95_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNR65530.1"
                     /translation="MKTDCEENNFKIIKEQGITYPKGIKAAGINCGIRQTRKDLALIY
                     SSEIASSAAVYTQNKFKAAPLWVTEENLSRSGGKLQAILVNSGVANACTGEQGLQDAR
                     ETVKYVSDSLQIKEEYVAVTSTGKIGEFLPMDKMRDGVKEAVSKLSLKGGTDAAEAIM
                     TTDTRKKEIAISFTLNEKEIRIGGIAKGSGMIHPNMATMLAFVTTDIAIESSLLQEAL
                     TEVISKTFNMVTV"
BASE COUNT          956 a          428 c          569 g          748 t
ORIGIN      
        1 aaaaatatcc ctgcaaacag ctgcttgcaa ggatttatag aacttgacct tattggctta
       61 ctgcaaaact agggagtttg gaaccctttt ttaattcccg cctgcagatg gattttggaa
      121 agatacaatt taattatggt ggacaggtca attttaataa ctggaaagaa tggcaggaaa
      181 accgaaaaga aatcctcttg cggattaatg acagactttc tttaaactgg cagattccca
      241 atcgtttcgg cttcactatt tcctggtaaa caaaatctct ctaatagatt tataaataaa
      301 cggaacaacc cccccctgaa tccttcagca ggtattaaga taagctataa attattttaa
      361 aaaatatttg acaaaacaaa aacattactt tattattatg cacaaaataa taaagattac
      421 caggtagaag taagtctgac cagatactaa ttaacagaga gagataagtt aggtgaaaat
      481 atctcagtgc aggttgactg aaaagaaagc ctaattatta aaataaaata aggatgaagg
      541 ttggttacct ttaaaaaccg agaatgagtg ggataagtta tttatccaat tccaggtggc
      601 accgcgagat tagtctcgtc ctttaaaatt attttaaaaa aagatagttg gggacgggat
      661 tttttgtttc ttacttgatt aaagacaaaa tacacctgaa ttctactgaa tgatttatag
      721 atgttggtgc tgatgaataa cttattcaag gtgggtggca ccgcgagaga cttatataaa
      781 attgtatctc gtcccttaag gtaatataaa acatactatt attatatata cgggacgaga
      841 ttttttattc ttaaatacgc ctaaaaatat atactgtata taggaaagga gtttaaaaat
      901 ggaaaaaata aaagttagta tcatcggggc aaccggttat tcggggaaag agttaatcag
      961 gatattgtta aaacatcccc ggataaagtt gatgcatctg gtctcagcca gttatgtggg
     1021 ccagaatatt tgtgaagttt atcctgaatt taccaatcag ctggacaaaa agcttataga
     1081 acttgatata gaaaagatag tacgggactc tgatgtggta tttaccgcct taccgcataa
     1141 tatttctctt gatattgtgc cagagctact gattaataaa aaccagatga aagttattga
     1201 cctcagtgct gatttccgac ttaaggattc cgacaactat actaaatggt ataaaaaaga
     1261 gcatagtgaa aagagcagag tatttttaaa agaggcagta tatggattgc cggaattata
     1321 taaagaggat atcaaaaagg caacactggt agccaatccg gggtgttatc ccaccagtgc
     1381 cattttgggg atggctcctt tgttagcgag acgtctggta gaagctgata atataataat
     1441 tgatgccaaa tcgggtacca gtggagcagg gagaaagcta tcactgggtt tacattttag
     1501 tgagtgtaac gaaagcttta aagcctataa gacaactatg cataaccata tccctgaaat
     1561 cgagcaggaa ttatctctat tgtacaatcc cgattccggt aaagatgaag gcgaaaaaaa
     1621 taaatatggt aagaaaaaca aagaaattat agtatctttt accccacatc ttcttcctct
     1681 cagtagggga atcctgtcta cctgctatct caacttgatt gaagattgta atgagcagga
     1741 tattgtaact ctgtatcaga atttttatca ggatgctcct tttgtcagaa tatttgagcc
     1801 acccaatcta ccggagatac gttttgccca attaaccaac tattgtgata taggatttac
     1861 catagataag agggtgggaa aaatcaaggt aatttcagtt attgataatc taactaaagg
     1921 agcatcaggt caggcggtac agaatatgaa tattatgttc aatcttccag aaacaatggg
     1981 attgttataa aggagaaagg agtaaaaaat gaaaacagat tgtgaagaaa ataattttaa
     2041 gataataaaa gaacagggca taacctaccc caaagggatt aaagcagccg gtataaactg
     2101 tggcataagg caaactagaa aagatttggc tttaatctat tcaagtgaga tagccagttc
     2161 cgcagcagtt tatactcaaa ataagtttaa agcggcacct ctctgggtca ccgaggagaa
     2221 tctctcccgc tctggtggca aattacaggc tatactggta aatagcgggg ttgccaatgc
     2281 ctgtactgga gagcagggat tacaagatgc ccgggaaacg gtcaaatatg tttcagacag
     2341 tttacagatt aaagaagaat atgtggcagt aacttccact gggaagattg gagagtttct
     2401 gccaatggac aagatgagag atggggtcaa agaggcggtg agtaaattaa gtttaaaggg
     2461 tggaactgat gcagcagagg caataatgac cacagatacc aggaaaaaag aaatagccat
     2521 aagcttcaca ttgaatgaga aggaaattag aattggtgga attgctaaag gttcggggat
     2581 gatacatcct aatatggcca ctatgttagc ctttgtgaca accgatatag caatagaaag
     2641 cagtcttttg caggaagctc tgacagaagt aatcagtaaa acctttaata tggtgactgt
     2701 t
//
LOCUS       DAOQWT010000188         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_41218_length_2700_cov_2.463138, whole genome shotgun sequence.
ACCESSION   DAOQWT010000188 DAOQWT010000000
VERSION     DAOQWT010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41218_length_2700_cov_2.463138"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKJ95_04465"
     CDS             complement(<1..271)
                     /locus_tag="PKJ95_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNR65531.1"
                     /translation="MRTILNNGIIITPIRIIKNGGIIIEKNSIVNLFDQSNDIKFGKS
                     DQIIDVKGHYISPGFIDIHTHGGFGYDYLDGSVESIIKASEGHMRY"
     gene            complement(366..1604)
                     /gene="nagA"
                     /locus_tag="PKJ95_04470"
     CDS             complement(366..1604)
                     /gene="nagA"
                     /locus_tag="PKJ95_04470"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNR65532.1"
                     /translation="MVKRIAFINGTLITPFREISKGFIIIKDNLIEKIGQMGQLEGTT
                     FPENTHIIDVNGLYVSPGFIDSHLHGAFGGSAMSATEKDLQIMAQGLVQCGTTSFLPS
                     TLSAPWDKVTGAVDCISGVMKRDLQGARILGIHLEGPYINLEQKGAQNPEYIYSPAPE
                     QYLPLLNNYPEIIRVTSAPEIPGGMELAQELRRRKIVAAIGHSSATYQQVLQAIENGY
                     SHVTHMFSSMSSIKRINGYRISGVIESTLLLDELTTELIADGHHLPPSLMKLALHCKG
                     MDKVCLVTDSMAAAGLGPGKYELGGLEVIVESDIPQEFEILTQENNYVAKLIDHSSFA
                     SSVSTMDKLIKNMIRHCELSIREAVQLVTYNAAKIQHLEDEIGILAENKRADIAVFDN
                     NLDIKITVVNGSILYMDFIY"
     gene            complement(1640..2377)
                     /locus_tag="PKJ95_04475"
     CDS             complement(1640..2377)
                     /locus_tag="PKJ95_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNR65533.1"
                     /translation="MKINISYPNIIISQTAAVKAAEMLNEAISKKGTATFVAATGASQ
                     FEFLDHLTKITNINWSKTTMFHLDEYIGIPEDHPASFRKYLKERLINKVHPSKVYLIS
                     GDAQDPKKECERLNGIISQHEIDVAFVGIGENGHLAFNDPPANFEVEKPYIIVELDAA
                     CRRQQLGEGWFKTFEEVPQRAISMSIKQIMKSKSIICTVPDERKAQAVKDCFSGDEIS
                     PDYPASILKKHDNCYVFLDDQSSKYLQ"
     gene            complement(2441..>2700)
                     /locus_tag="PKJ95_04480"
     CDS             complement(2441..>2700)
                     /locus_tag="PKJ95_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR65534.1"
                     /translation="KLKDRTSFAGSIATANKLLQTMMNYTGISLQDSIKMFTLTPARI
                     MKINQYKGLLAPGKDADIVVFDGDLNIKLVMVKGNIRFYSN"
BASE COUNT          844 a          527 c          393 g          936 t
ORIGIN      
        1 catatctcat atgtccttca gaagccttga taatagattc tactgaaccg tcaagataat
       61 catatccaaa accaccatgt gtatgaatat caataaatcc aggacttata taatggcctt
      121 ttacatcaat aatttgatcg gacttgccaa attttatatc attactttga tcaaatagat
      181 taacaatgct gtttttttca ataataatac caccattctt tattattcta attggagtta
      241 taataatccc attatttaat atcgttctca taagcaaatc ttttctacct taattattga
      301 agttagacac gtacttgtaa ttaactataa attacgaaat attaattctc tttaccgtat
      361 gtatcttaat aaataaagtc catatagagt atgctaccat ttacaacagt aattttaata
      421 tccaaattat tatcaaaaac agcaatatcc gctcttttgt tttctgccag aatacctatc
      481 tcatcttcca agtgctgtat cttggctgca ttgtaggtta ctaattgaac tgcttctcta
      541 atacttaact cacaatgtct tatcatattc tttattaatt tgtccattgt agatacacta
      601 gaggcaaaag aggaatgatc aatcaattta gcaacataat tattttcttg tgtaaggatt
      661 tcaaactctt ggggaatatc tgactctact attacttcca gtccccctaa ctcatactta
      721 cctggaccca acccggcagc ggccattgaa tcggtcacca gacaaacttt gtccattccc
      781 ttacaatgta atgctaactt cattaaactg ggtggaagat gatgaccatc agctattaat
      841 tctgtagtaa gttcatcaag tagtaaggtt gattctatta caccagaaat gcgataaccg
      901 ttaattcttt ttatactaga catactagaa aacatatggg tgacatgaga atagccattc
      961 tcaatcgcct gtaatacctg ttgataagta gcactagaat gacctatagc tgccacaatc
     1021 tttctccttc tcaattcctg tgccagctcc ataccgccgg gtatttccgg tgcagaagtt
     1081 actctgataa tttcaggata attatttaat aaaggtaggt attgttcagg agcaggagaa
     1141 taaatatact ctggattttg tgctcccttc tgttctaaat taatataggg gccttctaag
     1201 tgtattccta atatcctggc accttgcaaa tctcttttca ttaccccgga aatacaatct
     1261 actgcccctg taactttatc ccaaggagca gataatgtag aaggtagaaa agaagtagta
     1321 ccacattgca ctaaaccctg agccattatc tgcaagtctt tttctgttgc agacattgct
     1381 gaaccaccaa aagccccatg caaatgcgaa tcgataaaac cgggactaac atataatcca
     1441 ttaacgtcaa ttatatgggt gttttcagga aatgttgttc cttccaactg ccccatttga
     1501 ccaattttct caatcaaatt atctttaatt ataataaatc ccttagatat ttcacggaac
     1561 ggggtaatca gagtgccatt aatgaaagca attcgtttta ccaatttatt ttctcctatc
     1621 caactacaac aaattctaat tattgtaaat atttagaaga ctgatcatct aaaaatacat
     1681 aacaattatc atgtttcttc aaaatagatg cgggataatc tggtgaaatt tcatcacctg
     1741 aaaaacaatc ttttactgct tgtgctttac gttcatcggg aactgtacaa ataatagatt
     1801 tggatttcat aatctgcttt attgacatgg aaatggctct ttggggaact tcttcaaagg
     1861 ttttaaacca accttcccca agctgctgcc tcctgcaagc agcatctaat tccacaataa
     1921 tataaggttt ttctacctca aagttagctg gtgggtcatt gaaggcaagg tgcccgttct
     1981 caccaatacc cacaaaagcc acatctatct catgctgact gataattcca tttaatcgct
     2041 cacattcttt ttttggatcc tgggcatcac ctgatattaa atagacttta ctagggtgca
     2101 ctttgtttat taatctttcc ttgagatatt tcctgaaact ggcaggatgg tcttccggaa
     2161 taccgatata ttcatctaaa tgaaacatgg tggttttaga ccaattaata ttggttattt
     2221 ttgttagatg atctaaaaac tcaaattgtg atgcacccgt agcagcaaca aaagttgctg
     2281 tgcctttttt actgatagcc tcatttaaca tttccgctgc ctttacggca gctgtttgac
     2341 ttataattat attgggataa gaaatgttta ttttgatttt ttactgtttt cattacaatt
     2401 tttattttta tggaaaagtt aacattgttt atgatatgtt ttagttagag taaaatctaa
     2461 tatttccctt gaccattact aacttaatat ttaaatcacc atcaaaaaca acaatatctg
     2521 catccttacc aggagctagt aatcctttat attgatttat tttcattatt ctagcaggag
     2581 ttaatgtaaa cattttaata gaatcttgta aagaaatccc tgtatagttc atcattgttt
     2641 gtaataactt attcgcagta gcaatactac cagcgaatga tgtcctatcc ttaagttttg
//
LOCUS       DAOQWT010000189         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_41299_length_2696_cov_3.289284, whole genome shotgun sequence.
ACCESSION   DAOQWT010000189 DAOQWT010000000
VERSION     DAOQWT010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41299_length_2696_cov_3.289284"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /locus_tag="PKJ95_04485"
     CDS             <1..115
                     /locus_tag="PKJ95_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNR65535.1"
                     /translation="SFNKKSYHPAELMNPEKCTGCAFCAMTCPDVAIEVYK"
     gene            163..1218
                     /locus_tag="PKJ95_04490"
     CDS             163..1218
                     /locus_tag="PKJ95_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNR65536.1"
                     /translation="MEERLLMKGNEAIGEAAIHAGCRYYFGYPITPQSELIAYMAKRL
                     LKIKGATFIQAESEIAAINMVYGASAAGGRVMTSSSSPGISLKQEGISYLACAELPCV
                     IVNIMRGGPGLGSIQPAQGDYFQATKGGGHGDYHLIVFAPSSVQELVDLTIRAFSLAD
                     KYRNPAMILGDGLLGQMMEPVQFKEFDEKNIPSKEDWALGSSHGRKPRRIVPFDLDPN
                     ELEKIVNKLGQKYQDMQKEVEYEVVNIDNPDIVIVAYGSIARIVKTTIQKAKQEGINI
                     GLVRPITLYPFPYAAIEEVANKVDKFLVTEMSLGQMVEDVKLAVKGKSQVSFYGRTGG
                     VVISPIEILQEVKKLLD"
     gene            1288..2034
                     /locus_tag="PKJ95_04495"
     CDS             1288..2034
                     /locus_tag="PKJ95_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNR65537.1"
                     /translation="MKQVFTKPKSLTDVPFTYCPGCHHGIIHRLVAEAMDDLNIAEDT
                     IGIAPVGCAVFAYLFFECDMIVAAHGRAPAIATGIKRVKPDNIVFTYQGDGDLASIGT
                     AEIVHAANRGEKITVIFVNNAIYGMTGGQMAPTTLEGQITQTSPYGRDSKTMGMPIRI
                     CELLSTLNSPAYIARVALTKPAYIVKAKKAIKKAFQYQLDGKGFALVEVLSTCPTSWG
                     LSPKEANEWLNEKMIPYYPLGEFKNVEEVS"
     gene            2035..2574
                     /locus_tag="PKJ95_04500"
     CDS             2035..2574
                     /locus_tag="PKJ95_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNR65538.1"
                     /translation="MLDEIIIAGFGGQGILLMGRLIAHAGMLEGKHVAWMPSYGPEMR
                     GGTANCTVIISSEEVASPVVPNPLSLIAMNLPSLDKFEPLVEKGGTIIVNQSLISRNI
                     YRDDVEVVKVPANDIANELGNLRVANMVALGSYIAKSKSVKMQTVFEALEQTLGKGSE
                     KIIELNKEALKRGEKIIIG"
BASE COUNT          889 a          428 c          592 g          787 t
ORIGIN      
        1 atcatttaac aaaaaaagtt atcatcctgc tgagttgatg aacccggaaa aatgcaccgg
       61 atgtgctttt tgtgctatga cctgtccaga tgttgctata gaagtgtaca aataagaaga
      121 gcaaaaactt ctatctaaat ttttaaggag gattgattat taatggaaga aagattatta
      181 atgaaaggga atgaggcaat tggagaggct gctatccatg caggatgccg ttattatttt
      241 gggtacccca taacaccgca gagcgaattg attgcttaca tggctaaacg cttgttaaaa
      301 ataaaaggcg ctacttttat tcaagccgaa agcgagattg ctgcgattaa catggtttat
      361 ggggcttcgg cagctggtgg cagagtaatg acttcttcat ccagtccggg aattagttta
      421 aaacaggaag ggatttctta tcttgcctgt gctgaattac catgtgtaat agtcaatata
      481 atgagaggag ggcctggttt agggtctatt caaccagccc agggagatta ttttcaggct
      541 actaaagggg gtggacatgg tgattaccat ctcattgttt ttgctccatc ttcagtgcag
      601 gagctggttg acctaactat tagggctttt agtctggcag ataaatatcg taatccagct
      661 atgattttag gagatggatt attgggtcag atgatggaac cagttcaatt taaagaattt
      721 gatgaaaaga atatcccttc aaaagaagat tgggctctag gttccagtca tggaagaaaa
      781 ccaagaagaa tagtgccctt tgacctggac cccaatgagt tagagaaaat agtaaataaa
      841 ctcggccaga aatatcagga catgcaaaaa gaagtagaat atgaagtggt aaatattgat
      901 aacccggaca tagtcatagt tgcgtacggt agtatagcta gaattgtaaa gactactatt
      961 caaaaagcga aacaagaagg aataaatatt ggattagtta gacccattac tctttatcct
     1021 tttccgtatg ctgcaattga agaagttgcc aataaagtag acaagttttt ggtaactgag
     1081 atgagtctgg gacagatggt tgaagatgtt aaattggcag ttaaaggcaa gtcacaagtt
     1141 agtttctatg gcagaaccgg cggggtagtt atttctccaa tagaaattct gcaagaagta
     1201 aaaaaattat tagattagtt agtatagaat aacatcagat aattataaaa acattgttta
     1261 tgaaaaaata tgggggtaaa aaaattgatg aaacaagtat ttaccaaacc taaatcttta
     1321 acagatgtac cttttactta ttgccccggt tgccaccatg gtattattca ccgattagtt
     1381 gctgaggcaa tggatgattt aaatatagct gaagatacta ttggtattgc tccggtaggt
     1441 tgtgctgtat ttgcttacct attttttgaa tgtgatatga tagttgcggc tcatggaagg
     1501 gcaccagcaa tagctaccgg tataaagaga gtaaaaccag ataacatagt cttcacttac
     1561 caaggagatg gcgaccttgc ctctatagga actgccgaaa tagttcatgc tgctaataga
     1621 ggtgaaaaaa taacagtcat tttcgttaat aatgccattt atggtatgac tggcggccag
     1681 atggctccaa caacactgga aggccagata acccaaactt caccttatgg acgggattca
     1741 aaaactatgg gaatgccaat ccggatttgt gagttacttt ctaccttaaa tagccctgcc
     1801 tatattgcca gggttgccct gacaaaacca gcttatattg taaaagccaa gaaggctatt
     1861 aaaaaagctt ttcaatatca gcttgatggt aaaggatttg ctctggtaga agttctttct
     1921 acctgtccta ccagctgggg attaagcccc aaagaagcca atgaatggct aaatgaaaag
     1981 atgataccat actacccctt aggtgaattt aaaaatgtgg aggaggtatc ttaaatgtta
     2041 gatgaaataa ttattgccgg atttggtggt cagggaattc ttttaatggg aagattgatt
     2101 gcccatgctg gcatgttaga gggaaaacat gtagcatgga tgccttccta tggtcctgaa
     2161 atgcgaggag gaacggcaaa ttgtactgtg attatttctt cagaagaagt tgcctctccg
     2221 gtagtcccta atcccctttc tttaattgct atgaatttac catctttaga taaatttgaa
     2281 cctttagtag aaaaaggtgg tacgattata gttaatcaat cactcatttc tcgtaatatt
     2341 taccgagatg atgtagaggt ggtgaaagtt ccagctaacg acattgccaa cgaactgggt
     2401 aatttaaggg ttgccaatat ggtagcattg ggtagctata ttgccaaaag taaaagtgtg
     2461 aagatgcaaa cagtctttga agctttagaa cagacacttg gtaaaggttc agaaaaaatc
     2521 attgaactaa acaaagaagc acttaaaaga ggagaaaaaa ttattatcgg gtaagattaa
     2581 atatatttta aaaatttaac tgtcttttac cttaaacttc ctatttatgt gtatgtgctg
     2641 acttattaaa aagtcagcac ataaatttct tgggaggaag tagacggact gtatat
//
LOCUS       DAOQWT010000190         2691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_41426_length_2691_cov_2.371396, whole genome shotgun sequence.
ACCESSION   DAOQWT010000190 DAOQWT010000000
VERSION     DAOQWT010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41426_length_2691_cov_2.371396"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKJ95_04505"
     CDS             complement(<1..562)
                     /locus_tag="PKJ95_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNR65539.1"
                     /translation="MNISEKLRQRAKKEIKKIVLPEGEEPRMIKAAEKIYEESFAKLV
                     FLGNKKKIRDIANESNINFPESIEIIDPESSEKLHTYAEIYYDLRKNKGMTIEEANRL
                     LRDPLYFASLMVYQDDADGLVAGSINATGDVFRPALQTIKTSSNVNIVSSSFIMVVPG
                     CKYGENGIILFADCALFPDPNPEELAD"
     gene            673..1968
                     /locus_tag="PKJ95_04510"
     CDS             673..1968
                     /locus_tag="PKJ95_04510"
                     /inference="COORDINATES: protein motif:HMM:NF010191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNR65540.1"
                     /translation="MLILGIIAEYNPFHNGHYYLLQKSKKISGADFTLAVMGGNFLQR
                     GEPAFWNKWVRTKMALSAGIDLVIELPFVFASQDAHGFAQAGIRILNSLGIVDYITFG
                     CENDNLEIFFKLAELIRKDPPFFKKIIKEELKKGSSFPKIRERAILSFYQRYYQDSSE
                     VSINEIISILSNPNNILALEYMISLQSLKSTIEVVPIKRIGSGFSQEEYEGSYSSATA
                     IRKLVSQYYYLQDRYFLKELKAALPLSTFRIILEQLKEEINPIMFSNFEHTILNKIRS
                     ISISDLERINGVQEGLENKIKKAAISTGNIEELIVAIKSKRYTRTRVQRMIIHSLFNL
                     TRAEVQKFNKVGPLYCRVLGMSENGKIILQKAKSNSKLPIVLKLKNFYNHNKRSGNGL
                     VLKMLNYDILATDLYVLAYKKRELRIGGQDYTNHLYIKL"
     gene            complement(1984..2466)
                     /gene="coaD"
                     /locus_tag="PKJ95_04515"
     CDS             complement(1984..2466)
                     /gene="coaD"
                     /locus_tag="PKJ95_04515"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNR65541.1"
                     /translation="MIKAIYPGSFDPITNGHLDIMYRASKIFDKIIVVIAENSKKKSL
                     FSTEEKMDMILNVIDKFNNIEVQSFRGLLIECVRSNEANIILRGMRAISDFEYESQFA
                     LINKKMAPEIETIFMVTSTRFSYLNSSIVKEIASLNGCVNELVPPYVVKKLKNKYGFN
                     "
BASE COUNT          936 a          432 c          400 g          923 t
ORIGIN      
        1 tatcagctaa ttcctcgggg ttagggtcag ggaataaagc gcagtctgca aataatatta
       61 ttccattttc tccatattta cagccaggta ccaccataat aaaagagctg gacacaatgt
      121 taacattact cgaagtctta atggtttgca aggcaggtct aaaaacgtca ccagtagcat
      181 taattgaacc agctaccaaa ccatcggcat catcctgata aaccattaat gaagcaaaat
      241 atagaggatc tctcaacagt ctgttagctt cttcaatagt cattccctta ttctttctta
      301 aatcataata aatttctgca taagtgtgta gtttttcaga tgattcgggg tcaataatct
      361 caatgctttc tggaaaatta atgttggact cattggctat atctcttatc ttttttttat
      421 tacccaaaaa tactaacttt gcaaaacttt cttcataaat tttttcagca gccttgatca
      481 ttcttggctc ttccccttcc ggtaaaacaa tctttttaat ctcctttttt gccctctgcc
      541 ttagtttttc agaaatattc ataatgatac attgcctcct ttaaaatttt ttattataaa
      601 tataaaaaga atacaaaata tcataaatac cgtataatta taacatataa caactaatat
      661 atcgaggaag atatgctaat tttgggcatt attgctgaat ataatccttt ccataatggc
      721 cattactatc ttttacaaaa atctaaaaaa atttcaggag ctgattttac tttggcagtt
      781 atgggcggta attttctaca gcggggggaa ccagcttttt ggaataaatg ggttcgcacc
      841 aaaatggctt tatctgccgg cattgatctg gttatcgaac ttccttttgt ctttgccagc
      901 caggatgcac atggttttgc gcaggcagga attagaattt taaactcctt aggaattgtc
      961 gactatataa cttttggttg tgagaacgat aatctggaaa tattctttaa gctagcagaa
     1021 ttgattagaa aggatcctcc cttttttaaa aaaatcataa aagaagaatt aaaaaaagga
     1081 agcagttttc ccaaaatacg ggaaagagca attctgtctt tttatcaaag atattaccaa
     1141 gattcatcag aggtatcaat aaatgagatt attagtattt taagtaatcc aaataatatt
     1201 ctagctttag agtatatgat atctctgcaa tcattaaaaa gtactataga agtagtacct
     1261 atcaaaagaa ttggctcggg tttttctcaa gaagaatatg agggcagtta ttccagtgcc
     1321 actgctataa gaaaacttgt cagccaatat tattatttac aagacagata ttttttaaag
     1381 gaattaaaag cagccttgcc tctttcgacc tttcggatta ttttggagca attaaaagaa
     1441 gaaattaatc ctattatgtt ttcaaatttt gaacatacta tacttaataa aatcagaagt
     1501 atctctattt ctgatttaga gagaattaat ggtgttcaag aagggttaga aaataaaatt
     1561 aaaaaagctg ctatttcaac agggaacatt gaagaattaa ttgtagccat aaaatctaaa
     1621 cgttataccc gtacaagggt acagagaatg attattcata gtctatttaa tttaaccaga
     1681 gcagaggtgc agaaatttaa taaagttggg cccctttatt gtagggttct gggaatgagt
     1741 gaaaatggta aaataattct acaaaaagct aaatcaaatt ccaaattacc tattgtttta
     1801 aaactaaaaa atttttataa ccataacaaa aggtctggca atggtcttgt tttaaaaatg
     1861 ttaaactatg acatattagc aactgatctt tatgttctcg cttataaaaa aagagagttg
     1921 cggattggtg gtcaggatta taccaatcat ttatatataa aactataaga aaacattttg
     1981 aagctaatta aatccatatt tattttttaa cttttttact acataaggag gaactaattc
     2041 gtttacacaa ccgtttaaag aagctatctc tttcactata cttgagttca aataagaaaa
     2101 tctagtactg gttaccataa aaattgtttc tatctcagga gccatttttt tattaattag
     2161 cgcaaattga gattcatatt caaaatccga aattgctctc atacccctaa gaataatatt
     2221 ggcctcatta gatctaacac attctattaa taatcctctg aatgattgaa cctcaatatt
     2281 attaaattta tcaataacat tgaggatcat atccattttc tcttctgtgg aaaagagtga
     2341 tttttttttg gaattttcgg caattaccac aataatttta tcaaatattt tagaggctct
     2401 gtacattatg tcaagatggc cattggtaat agggtcgaaa cttcctggat aaatagcttt
     2461 tatcatataa atctcctttt agatgtagtt atcgttacca aaaatggtga tttttgaact
     2521 tccatatttt ttctctttac aataaaaaat atttgaaacc ttctgttaaa agccggtcaa
     2581 ataatggaaa gttaatttat ctcttattaa aaaacttcaa aacgaacaaa cccaatggaa
     2641 aaatctaaaa actcattttt tggatgctta atacagatct ccttttttac a
//
LOCUS       DAOQWT010000191         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_41443_length_2690_cov_2.551803, whole genome shotgun sequence.
ACCESSION   DAOQWT010000191 DAOQWT010000000
VERSION     DAOQWT010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41443_length_2690_cov_2.551803"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PKJ95_04520"
     CDS             complement(<1..495)
                     /locus_tag="PKJ95_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65542.1"
                     /translation="MVLIWLVALFSFLITFIAFPVVIPRLKKAGITGKNMNSEIQEEI
                     PEMGGLVIVAGFCGGIILIIFLKIFFGFLPDVYLVTLLATLSTVLIVALIGIFDDLIS
                     MRQIIKAFMPLLAALPLVAIEIGDRILKIPFIGFINFGIWYPLVLVPLGVTGATNAFN
                     MLAGF"
     gene            complement(541..1932)
                     /locus_tag="PKJ95_04525"
     CDS             complement(541..1932)
                     /locus_tag="PKJ95_04525"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012194310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNR65543.1"
                     /translation="MKVIILAGGAGSRLWPLSRERFPKQFIKLQGREFSLFQETFNRS
                     VRLASLNDIYVITNKEYKFLVMGEIEDLGYKFPEKNILVEPEAKNTLPAIFAGVNEVI
                     KKGKDSLSESIVVFPSDHLIEKSIEFTEIIKSSEYLTGDSLVTFGIKPANPNTGYGYI
                     SPGEVKGNGYSVKEFKEKPDYQTAIEYIKDGYFWNSGIFLFNSEIFLQEAVRYTPEVL
                     NAFEGSKNLEEAFGKIEGKPSIDYGIMEKSKRVVVVPVDIGWNDLGSFDTFYEVFKCD
                     GKNNIVGKEHLLVNCSNNLVYSEEGKLVVTIGIDDVIIVDNKDALLVCKKEQSQKVKE
                     VVDELKRKKDLRVEYHLWDYRPWGSYQILEEEKNAFKVKRLKINPGKKISYQIHQHRS
                     EHWIVVRGKAKVTLDGEHKMVNKGESIDIKAGQKHRLENVGESVLEIIEVQMGEYLEE
                     DDIVRFDDEYGRK"
     gene            complement(2020..>2690)
                     /locus_tag="PKJ95_04530"
     CDS             complement(2020..>2690)
                     /locus_tag="PKJ95_04530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR65544.1"
                     /translation="RYYTVRSGMELSNFFQDNEPGIEAIRKEFSIGPDDFVIGNISRL
                     ELLKGHSLLLRAFKILREQEKDKSRRIILMIIGDGEEKDNIYQDIKKLGLEKSVILKG
                     YRNDIPNILATIDLLVHTSFREGLPRVLVQAAATGIPMVAFNVDGIPEIIRDGYNGFL
                     VEPKNINQLVEKITIYLRKPELLKMHGDNSKKVVKNRWTIEGMVKGTDKIYSKLIEEK
                     ISSI"
BASE COUNT          841 a          624 c          311 g          914 t
ORIGIN      
        1 aaaccctgcc agcatattaa aggcattggt agcaccggta actcccagag gtaccaagac
       61 cagaggatac cagattccaa aatttataaa accaataaaa ggaattttta aaattctatc
      121 accaatctcg atcgctacta agggtaaagc tgcaagcaaa ggcataaaag ccttgataat
      181 ttgtctcatg gagataaggt catcaaatat tccaattaat gccactatta acacggtaga
      241 aagagtagcc agcagggtaa caagataaac atcaggtaaa aaaccaaaga atattttaag
      301 aaaaataatt aagataatcc ctccgcaaaa accggctact attaccaatc cacccatttc
      361 cggtatctct tcctgtattt cactattcat attcttacca gtaattcctg ctttttttaa
      421 gcgtgggata actactggaa aagctataaa agtaatcaaa aaagaaaata aagccaccaa
      481 ccatattaaa accattttat ttacctacat gcatatcttc atataaaaca cattctcatt
      541 tcattttctg ccatactcat catcaaatct cactatatca tcctcctcca aatactcacc
      601 catctgcacc tcaattatct ccagaaccga ttctcccaca ttttctaacc tatgtttttg
      661 cccggcttta atatcaatac tttcaccttt atttaccatc ttatgttcac catccaaagt
      721 cactttagct ttccccctta ccacaatcca atgttcactt ctatgctgat gtatctgata
      781 gcttatcttt tttccgggat ttatctttaa ccttttaact ttaaaggcat tcttttcctc
      841 ttcaagtatc tgatagcttc cccaggggcg gtaatcccat aaatggtatt caactctcag
      901 gtcttttttc cttttcagct catctaccac ttcttttacc ttttgagact gctctttctt
      961 gcaaaccagc aatgcatcct tattatcaac aataattaca tcatcaatac ctatggtaac
     1021 taccaatttc ccctcttcag aatagactaa attattacta cagtttacta acagatgctc
     1081 tttaccaaca atgttatttt ttccatcaca tttaaatact tcataaaaag tgtcaaaact
     1141 tcctaaatca ttccaaccaa tatcaacagg caccaccaca actcttttgc tcttttccat
     1201 tattccgtaa tctatggaag gcttcccttc tatcttacca aaagcttctt ccaggttttt
     1261 acttccctca aaagcattta aaacctctgg agtatacctt actgcctctt gcaaaaatat
     1321 ttcactatta aacaaaaaga tcccactgtt ccaaaaatat ccatctttta tatactctat
     1381 cgcagtctga taatcaggtt tttccttaaa ttcttttacc gaataaccat taccctttac
     1441 ttcacctggt gagatgtatc cataaccggt attaggatta gccggtttaa taccaaaagt
     1501 gaccagcgaa tctccggtta aatattcaga agatttaatt atttctgtaa attctatact
     1561 cttttcaatc aaatgatcag aagggaacac cacaatagac tcagaaaggc tatcttttcc
     1621 cttttttatg acctcgttaa ctccagcaaa aattgccggt aaagtatttt tagcttccgg
     1681 ttcaaccaat atattctttt caggaaattt atatcctaaa tcttcaatct ctcccattac
     1741 taaaaatttg tattctttat tggtaatcac ataaatatca tttaaactag ctaaccggac
     1801 actgcggtta aaagtctcct gaaacaaaga aaattctcta ccctgtaatt taataaattg
     1861 tttggggaaa cgttcacgac ttagaggcca tagtctggaa ccagcgccac ctgccagtat
     1921 tatcaccttc atctgctcac cttctttcca tttattctga aaataatgta aagatgtaaa
     1981 aacgattggg tcaatatccc aattccaatt gtacattaac taaatagagc taattttttc
     2041 ctcaatcagt ttagaatata ttttgtctgt tccttttacc atcccttcaa tggtccatct
     2101 attttttact acttttttac tattatcgcc atgcatttta agcaattctg gtttcctcaa
     2161 ataaattgta attttttcta ccaactgatt tatatttttg ggttcaacta aaaacccatt
     2221 atagccatcc cgtattattt ctggaatacc gtctacatta aaagcgacca tgggtatccc
     2281 tgttgcagct gcctgtacca atacccgggg aagaccttct ctgaaggaag tgtgtactaa
     2341 aagatctata gtagcaagaa tatttggaat atcattacga tatcctttta aaataacact
     2401 tttttctaac cccaatttct tgatatcctg gtaaatatta tctttttctt caccatctcc
     2461 aataatcatc aaaatgattc ttctgctttt gtctttttcc tgttccctca atattttaaa
     2521 agctctcagt agcaggctat gcccttttaa taactcgagt cgacttatat tccctattac
     2581 aaaatcatct ggaccaatag agaattcttt tcttattgct tcaataccgg gttcattatc
     2641 ctgaaaaaaa ttacttagct ccattccact cctaaccgta taatacctct
//
LOCUS       DAOQWT010000192         2677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_41698_length_2677_cov_8.763539, whole genome shotgun sequence.
ACCESSION   DAOQWT010000192 DAOQWT010000000
VERSION     DAOQWT010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41698_length_2677_cov_8.763539"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PKJ95_04535"
     CDS             complement(<1..969)
                     /locus_tag="PKJ95_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNR65545.1"
                     /translation="MSRNTTNDKCYRIANVEQAKKVSLNYLKNIELDKVLAFGLPEID
                     DRYHYWRVPLLDGNNKIGEIVIDAYTSFVLGGKTTKKDVLEARLLGRKTKTKSKNNNK
                     NDIPKISTLRNTVGQGDSEELLAEIPAESVDLIFTSPPYFNARPEYSDYINYEDYLLK
                     MRKIIYQSHRVLNEGRFFVINIAPVLLRRSSRNESSKRLAVPFDFHRIFIEEGYDFID
                     DILWVKPEGAGWATGRGRRFAADRNPLQYKAVPVTEYILVYRKRTDKLIDWHIRKHPN
                     QEIVKESKIENGYEVTNLWKIHPAHNKQHPAVFPLELAEKVIKYYSF"
     gene            complement(1204..1332)
                     /locus_tag="PKJ95_04540"
     CDS             complement(1204..1332)
                     /locus_tag="PKJ95_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65546.1"
                     /translation="MINNQNDIINQAKTLTTEKRKVILKSLGISGETGQILRKDLI"
     gene            complement(1472..2332)
                     /locus_tag="PKJ95_04545"
     CDS             complement(1472..2332)
                     /locus_tag="PKJ95_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65547.1"
                     /translation="MAELTTWSVKMTDTLKEKISGSLQESGLTGKEFMEVLLNTYEQV
                     KKDRPEITPDLDELETLTKRICAIYVNVGERITTLLNDKDNSYKSLVTEKTDLARTLQ
                     KRITELENALKEKITIAKELAAEKDKIQEEKINLELRQQKEIQQLAEINQSNKSLIEE
                     YRQKNDTLTGLLAEYETYKNQIQELKKEVNLEKIKRAGAEDKFKKAEDTLNKNKEKLF
                     QTEERYKVQIESIIEQQSFEKEKALLEQEKSLRNELQAEREEYNNRIKALLDEIEARN
                     KALNKKSEVN"
BASE COUNT          754 a          476 c          360 g         1087 t
ORIGIN      
        1 aaaagaataa tattttataa ctttttctgc caattctaaa ggaaatactg cagggtgttg
       61 tttattatgt gctggatgaa ttttccataa attcgtcact tcatatccat tttcaatttt
      121 agattctttt acaatttctt ggttaggatg ttttctaata tgccaatcaa tcaatttatc
      181 agttcttttt ctatatacta atatatattc tgtaactggt actgctttat attgtaaagg
      241 gtttctatct gctgcgaagc gtctccctct accagttgcc caccctgctc cttcaggttt
      301 cacccatagt atatcatcaa taaaatcata gccttcctca ataaatattc tatgaaaatc
      361 aaatgggact gctaaacgtt ttgaagattc attacggcta gaacgtctta ataaaactgg
      421 tgctatattt attacgaaga aacgtccttc atttaaaaca cgatgggact gataaattat
      481 ttttcgcatt tttaataaat aatcttcata atttatataa tcagaatatt caggacgtgc
      541 attaaagtaa gggggtgagg taaatataag atcaacggat tccgctggta tttctgctaa
      601 aagttcttca ctatcacctt ggccgacagt atttctcaaa gtagatattt taggaatatc
      661 atttttatta ttattttttg atttagtttt tgtttttcta cctaataacc tagcttctaa
      721 aacgtctttt ttggttgttt ttcctcctaa aacaaaacta gtgtatgcat caataacgat
      781 ttctcctatt ttattatttc catctagcaa gggtactctc caataatgat atctatcgtc
      841 tatttccggt aagccaaaag ctaaaacctt atctaattca atatttttta aataatttaa
      901 agatactttt tttgcctgtt ctacattagc aattcggtag catttatcat tagtagtatt
      961 tctactcaat tctacaccta ctctgtttaa aatttatgat tacaaataaa ctttttgaca
     1021 ttattctaat ttatccttta ttgggtgatc cgggggaaac aggtggaatg ttacgcaggg
     1081 atatactcag aacccttatg gaacaataag tttttcattt ttttatttcc caaaaattta
     1141 gtcattttag accctaaaaa acggggaata aaaaagtaat agaaagtgtt atctggccta
     1201 gttttatatt aaatctttac gtaatatttg ccctgtttcc cccgaaatac ccaacgattt
     1261 taaaataact tttctttttt ctgttgttag tgtttttgct tgattaatta tatcattttg
     1321 attatttatc ataaactcat aactcctcat tatatatcag aatgttgtat gaaagataac
     1381 attctatttt taaatataaa atttcttgta aatgaaatat aaaataaagt ataattaaac
     1441 aagttatata cttgatcata ttaatttttt cttaatttac ttcgcttttt ttatttaaag
     1501 ccttgttcct tgcttcaatc tcatcaagta aagcctttat cctgttatta tattcttccc
     1561 tctctgcctg cagttcattc cttaaagatt tctcctgttc caggagggcc ttttctttct
     1621 caaaggactg ctgctctatt atgctctcta tctgcacctt atatctctcc tctgtctgga
     1681 ataatttctc tttgttttta tttagtgtat cttctgcctt cttgaactta tcttctgctc
     1741 ctgctctttt aatcttttcc aggtttactt ccttctttag ctcctggatc tgatttttat
     1801 atgtctcata ctctgccagg agtcccgtta aagtatcatt tttctgcctg tattcctcaa
     1861 tcaaagattt attactctgg tttatttctg caagctgctg gatctctttc tgctgccgga
     1921 gctccagatt aatcttctct tcctggatct tatctttttc tgctgcaagc tctttagcaa
     1981 tagttatctt ttctttaaga gcattttcaa gctctgttat tctcttctgt aatgtgcggg
     2041 ctaaatcagt tttttctgtg acaagagatt tataggaatt gtctttatca ttcagcaggg
     2101 ttgttattct ctctccaaca tttacgtata tagcacagat tctttttgtc agggtttcca
     2161 gttcatccag atccggcgtt atctccggcc tgtctttttt cacctgctca taggtattta
     2221 acaggacttc catgaattct ttgcctgtaa gcccgctttc ctgcaggctg cctgatattt
     2281 tctctttcaa agtatctgtc attttaactg accaggttgt tagctctgcc atattgataa
     2341 cctccttatg acttatgaat agtttactat tttattaagt aaaccttagt atttatagta
     2401 ttctatagtt tactgtaaaa tccttctttg tttttaaata attaattttt tattttatta
     2461 taaatgtcat acataaggtt tattttattc gtttttccgt tctacttgcc cgaaatttca
     2521 ccagagataa gaacaattaa ggaacgccaa aaaagtgaaa aatgtttaat tatataggaa
     2581 ttttatttgc aaaaaatgtt atgacgaaat tcaagtaaac cattgcaatt actagattat
     2641 tttgatataa ttataaatat aaaggaggtg taaaatt
//
LOCUS       DAOQWT010000193         2676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_41725_length_2676_cov_4.016024, whole genome shotgun sequence.
ACCESSION   DAOQWT010000193 DAOQWT010000000
VERSION     DAOQWT010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41725_length_2676_cov_4.016024"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..374
                     /gene="rpmE"
                     /locus_tag="PKJ95_04550"
     CDS             129..374
                     /gene="rpmE"
                     /locus_tag="PKJ95_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNR65548.1"
                     /translation="MKKGIHPKYGKAKVVCACGNTFETQSTKELIRVEVCSACHPFFT
                     GEQKIFDSAGRVEKFHRRYKMDQEQEENKQSQSEKED"
     gene            478..1071
                     /locus_tag="PKJ95_04555"
     CDS             478..1071
                     /locus_tag="PKJ95_04555"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNR65549.1"
                     /translation="MQETCSGGSIEVICGSMFSGKSEELIRRVRRVQIARKKVQIFKP
                     LIDMRYQPKYVYSHDGSKIEAVCIEKSAEILSEVKSDTDVVAIDEAQFFDRQIVEICQ
                     FLADQGKRVIVAGLDQDFRGEPFGSMPELLSLAEYVDKLHAICMVCGKPATRTQRLIH
                     GKPADYNDPIILIGARERYEARCRLHHEVTNKPSKTG"
     gene            1095..1778
                     /gene="thyX"
                     /locus_tag="PKJ95_04560"
     CDS             1095..1778
                     /gene="thyX"
                     /locus_tag="PKJ95_04560"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNR65550.1"
                     /translation="MKVQLLNYTRNPEETVAQAARLCYSNKNINELKEATEGEDCHRI
                     IKKIMDLGHYSVLEHASFTFGIEGISRVTSHQLVRHRIASFSQQSQRYVKVGGKQEYI
                     IPESVQKNKKELFLRFKELSQACFSFYQEMLREGILAEDARYILPQAITTSIVFTANS
                     RELIHLFRLRCCNRAQWEIRELAISMLKLVKEVAPGIFKNAGPPCLTGSCPEREMTCG
                     KPWKKEKDY"
     gene            1805..>2676
                     /gene="prfA"
                     /locus_tag="PKJ95_04565"
     CDS             1805..>2676
                     /gene="prfA"
                     /locus_tag="PKJ95_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNR65551.1"
                     /translation="MFEILEDIEKEYQTLNDELSQPKVIANHELFQKLAKRHSEISRM
                     AQLYLEYKKIEQEIAENEQLKKNEKDPELNEMIIEELELLHEKRDKLKNSLQELLNPE
                     DPRNKKNIIVEIRAGAGGEEAALFVSDLFRMYSRFAERNHWKVEVMDSNPTGIGGFKE
                     IIFGISGKNVFGSLKQESGVHRVQRIPVTEAGGRIHTSTVTVAILPEAEEIEMEINPN
                     DLKIDRYRSTGPGGQSVNTTDSAVRITHLPTGMVVTCQDEKSQHKNKAKALKILRARL
                     LDRLHHEQEEKIGQ"
BASE COUNT          935 a          443 c          597 g          701 t
ORIGIN      
        1 tacaatacct ccaaaaaagg ttaaaatgat attgatactt taaatcaaaa actataaata
       61 taagtttcag gaagaaatac ttccatgcga tttgagcata ttttattaag aatgaaggtg
      121 agatgttaat gaaaaaggga attcatccaa aatatggaaa agctaaagta gtctgtgcct
      181 gtggaaatac ttttgaaact caatctacaa aggagttgat aagggtagag gtctgttctg
      241 cctgccatcc tttttttacc ggagaacaga aaatatttga ttctgccggt agagtagaga
      301 agtttcacag aagatataaa atggatcagg aacaagaaga aaacaagcaa agccagagtg
      361 aaaaagaaga ctaaatatta ttttcacttt attaaagaat aaagaataaa aaacagggca
      421 aaggctctgt tttttattat aatctatttt gaaaaattaa ttggggagga aagcaggatg
      481 caagaaacct gttccggggg cagcatagag gtgatttgcg ggagtatgtt cagcggaaaa
      541 agtgaagaat tgatcaggag agttcgccgt gtacagattg cccgaaaaaa agtacagata
      601 ttcaaacccc tcatagatat gcgttatcag ccaaaatatg tctattccca cgatggcagt
      661 aagattgaag ctgtctgtat tgaaaagtca gcagaaatac tgagtgaagt caagtcagat
      721 accgatgtgg tagcaattga tgaggcgcaa ttttttgacc ggcaaattgt agagatatgc
      781 cagtttctgg cagaccaggg caagcgggta attgtagccg ggcttgacca ggattttcgg
      841 ggtgaacctt ttggttctat gcctgaactg ctatctctgg ctgaatatgt ggataaactt
      901 catgctatct gtatggtatg cgggaaaccg gctaccagaa cccagagatt gattcatggc
      961 aaacctgcag actacaatga ccccatcatt ttaatcgggg ccagggaaag atatgaggcg
     1021 agatgtagat tgcaccatga agtcaccaat aaaccgtcaa agacgggtta aataaatatg
     1081 ggaatttaat taatatgaag gttcaattat taaactatac cagaaacccg gaagagactg
     1141 ttgctcaggc agcccggcta tgttattcca ataaaaatat caacgagtta aaagaagcaa
     1201 cagagggaga agattgccat agaattataa agaaaattat ggatttggga cattattctg
     1261 ttctggaaca tgcctccttt acctttggaa ttgagggtat atcgagggtt acttcacatc
     1321 agctggtcag acatcgcata gcctcctttt ctcagcagag ccaaagatat gtgaaggttg
     1381 gaggaaagca ggaatatatt attcctgaaa gcgttcaaaa gaacaaaaaa gagttatttt
     1441 tgagattcaa agagctctcc caggcctgtt tttcttttta tcaggagatg ttaagagaag
     1501 gaatccttgc cgaagacgca cggtatattt tgcctcaggc tattaccacc agtattgtct
     1561 ttaccgcgaa ttcccgtgaa ttgattcatc tcttcagact gagatgctgt aacagggcac
     1621 aatgggaaat aagggagctg gcaatttcaa tgttgaaact ggtaaaagag gttgctcccg
     1681 gtatttttaa gaatgccgga cccccttgcc tgactggctc ctgcccggag agggaaatga
     1741 cctgtggtaa accctggaaa aaagaaaagg attattaaga ggatattaaa aggcagtgat
     1801 taatatgttc gagatattag aggatattga aaaagaatac cagactttaa atgatgagtt
     1861 gtctcagcct aaagttattg ctaaccatga actatttcaa aaattagcca aaaggcatag
     1921 tgaaatttcc agaatggctc aactttacct ggaatataaa aaaattgaac aggaaatcgc
     1981 tgaaaatgaa caacttaaaa aaaatgaaaa agaccctgaa ttaaatgaga tgataattga
     2041 agaattagaa ttactccatg aaaaaagaga taagttaaaa aattctttac aggaattgtt
     2101 aaatcctgaa gaccccagaa ataagaaaaa tattattgta gaaattagag caggtgccgg
     2161 tggagaagag gcagcacttt ttgtcagtga tctgtttaga atgtattccc gttttgctga
     2221 aagaaatcat tggaaagtag aagtaatgga ttccaacccc actggtatcg gcggctttaa
     2281 ggaaattatc tttggtattt ccggtaaaaa tgtctttggt tctttaaaac aggaaagtgg
     2341 agtacataga gtgcagagaa ttccggtaac tgaagctggg ggtagaatcc atacctctac
     2401 cgttaccgtt gctatcttgc cggaagctga agagattgag atggaaatta accccaatga
     2461 tttaaaaatt gaccggtatc gttcaaccgg ccccggtggc cagagtgtta atactaccga
     2521 ttctgcggtt agaattactc accttcctac cggaatggta gttacctgcc aggatgaaaa
     2581 atctcaacac aaaaataaag ccaaagcttt gaaaatactc agagcacggc tcttagatcg
     2641 actgcatcat gaacaggaag aaaagatagg acaaca
//
LOCUS       DAOQWT010000194         2663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_42040_length_2663_cov_2.582822, whole genome shotgun sequence.
ACCESSION   DAOQWT010000194 DAOQWT010000000
VERSION     DAOQWT010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42040_length_2663_cov_2.582822"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PKJ95_04570"
     CDS             complement(<1..473)
                     /locus_tag="PKJ95_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR65552.1"
                     /translation="MKKQKEKICTIIALIILFILVYFAQNRLDAYKIRILNLGAIYVT
                     LGVSLNLIYGFTGQFSLGHAGFMAIGAYVTALLVLPPYYKEVSFILEPLIWPFTVIHA
                     PFIIALFLSGLFAALVGFLIGLPVLRLKGDYLGIATLGFAEIIRVIANNLPHITNG"
     gene            complement(478..1371)
                     /locus_tag="PKJ95_04575"
     CDS             complement(478..1371)
                     /locus_tag="PKJ95_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR65553.1"
                     /translation="MTINLLLQNIANGLSLGSLYALIAIGYTMVYGILRLINFAHGEI
                     FMLAPYFLYFGILIFHLPWWASTIVAIGLTAFTGMLSERIAYKPLRDAPRISALISAI
                     GVSFFLQNLAVVVFSGIPKSVHRPIFFTKIYQFGEIRIQSTLFASIGVSIIFLSLLLY
                     IIYQTKTGLAMRAISRDIETSRLMGVGVNRAISITFAIGSALAAVGGILWSLRFPQIF
                     PTMGMIPGLKAFIAAVVGGIGSIPGAMLGGLILGMSEILFVTFLPALSGYRDAFVFFI
                     LIIILLVKPDGILGIKVQEKV"
     gene            complement(1489..2610)
                     /locus_tag="PKJ95_04580"
     CDS             complement(1489..2610)
                     /locus_tag="PKJ95_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR65554.1"
                     /translation="MKKNLIILFIAGVILLTGSLALAQDVVKFGVAEPMTGAMAVGGE
                     LCWRGYELALEQTPTVLGKKIELILVDNKSDKVESANAVSRLIERDGVVAVFVSYGSG
                     MAIPGGEVANKAGIPLISGTATNPLVTQGKEYVFRACFIDPFQGQVMAQYAYETLGFR
                     KSALLVDIAQDYSVGLANFYKRTFEQLGGKIVSDTKYNTGDQDFSAQLSQIIASGAEA
                     LFFPGYFGDVALIAIQGRELGFTGQYLGGDTLHNPVLIELAKDAAEGLICSTFFAEDQ
                     PATPESEKFAKLFREKYNLAPDAVSILCYDSYNLLVDAIERANSFDPVAIKNALATTK
                     DFQGAGGSITIDENGDAIKPAVLVKIVNGDYKYEATVYP"
BASE COUNT          898 a          560 c          455 g          750 t
ORIGIN      
        1 ccattagtaa tatggggtaa attgttggcg atgactctga ttatctcagc aaatcccaga
       61 gtggcaattc ccaaataatc acctttcaat ctcaataccg gtaagccaat caaaaaaccg
      121 accagggcag caaataatcc actcaggaat aaggcaatta taaatggagc atgaatcaca
      181 gtaaaaggcc aaatcagagg ttccagtata aaactcactt ccttgtagta gggaggaaga
      241 actaaaagag ctgttacata ggctccaata gccataaaac cggcatgacc gagagaaaat
      301 tgtcccgtaa atccatatat caaatttaaa cttactccca gagtgacgta tatagcacca
      361 agattaagaa ttcttatctt gtaggcatct aatctgttct gggcaaaata aaccaatata
      421 aaaagtatta ttaaagcaat tatggtacat attttttcct tctgtttttt cataatctta
      481 caccttctcc tgtaccttta tgcccaaaat gccatcaggt tttactaata atattattat
      541 tagaataaaa aatacaaaag catctctgta acctgacagt gctggtaaaa atgttacaaa
      601 taagatttca gacataccca gaatcaaacc tcccagcata gcacccggaa tacttccgat
      661 tcctccaact acagcggcaa taaaagcttt taagccaggt atcattccca tagtaggaaa
      721 tatctgcggg aacctgagac tccacaaaat gcctcctact gctgccaggg cggaaccgat
      781 agcaaaagtt atcgaaatag ccctgttcac tccgactccc atcaatctgg aggtctcaat
      841 atctctggat atggctctca ttgctaaacc agttttggtt tgataaataa tataaagtag
      901 aagagataag aatataatag atactcctat ggaagcaaag agagtagatt gaatacgaat
      961 ctcaccaaat tgatatattt tggtaaaaaa tatagggcga tgaacagatt taggaatacc
     1021 ggaaaaaact acaacagcca gattctgtaa aaagaaagac acaccgattg ctgaaatcaa
     1081 agctgaaatt ctgggtgcat ctcttaaagg tttataagca atcctttcag ataacatacc
     1141 agtaaatgca gttaagccaa tggccacaat tgttgaagcc caccagggca gatgaaaaat
     1201 taatattccg aaatataaaa aataaggagc aagcataaat atttctccat gggcaaagtt
     1261 tattaatctt aaaataccat agaccatagt atatcctatg gcaattaagg catataagct
     1321 tcccagagat aggccattag cgatattctg caaaagcaga tttattgtca tggatttctc
     1381 cttgtcatta ttggatattg actataaaca aattacacta ataggcaaaa gagataggac
     1441 ataccctatc tcttttgctt ttattataag ttaatcagct gcagaaaatt aaggatagac
     1501 agtggcttcg tatttgtagt ctccattcac aatttttacc agtactgcgg gtttgatagc
     1561 atcgccattt tcatcaatgg tgatggagcc accagcaccc tggaagtctt ttgtagtagc
     1621 taaggcattt ttaattgcta ccggatcaaa tgagtttgct ctttctatag catcaacaag
     1681 cagattatag gaatcatagc aaagaatgga aactgcatca ggcgctaaat tatatttttc
     1741 tctaaagagt ttagcaaact tttcagattc aggtgtggca ggctgatctt cagcaaagaa
     1801 ggtgctgcaa atcaaaccct cagcagcatc tttagccagt tctattaaaa ccggattatg
     1861 cagagtatca ccgcctaaat attgaccggt aaaccctaat tctctacctt gaatggcaat
     1921 cagggcaaca tctccaaaat aaccagggaa gaagagggct tctgctccag aggcaataat
     1981 ctggcttaat tgtgcactaa aatcctggtc accggtatta tatttggtat cactgacaat
     2041 ttttccaccc aattgttcaa aggttctttt gtagaaatta gctaaaccaa ccgaatagtc
     2101 ttgagcaata tcaactaaaa gagcagattt tctaaatcct aaggtttcat aagcatattg
     2161 agccataacc tgtccctgga aaggatcaat aaagcaagca cggaacacat attccttacc
     2221 ctgggtaacc agtggattgg ttgccgtacc agaaattaaa ggaattcctg ctttattagc
     2281 tacttctcct cctggaatag ccataccact gccgtaacta acaaaaacgg ctaccactcc
     2341 atctctttca ataagtctgg aaaccgcatt agcagactca actttatcgc tcttattatc
     2401 caccaggatt aattcaattt ttttgcctaa tactgtggga gtttgctcca gggctagctc
     2461 ataaccacgc cagcaaagct ctcctccaac tgccatggca ccggtcattg gttctgcaac
     2521 accaaattta acaacatcct gtgctaaagc caggctgcct gtcagaagaa tcactcccgc
     2581 tataaaaaga ataattaaat tttttttcat ttcttctctt cctttctttt atattatcta
     2641 ttggactact cattcagttt atg
//
LOCUS       DAOQWT010000195         2661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_42084_length_2661_cov_3.273599, whole genome shotgun sequence.
ACCESSION   DAOQWT010000195 DAOQWT010000000
VERSION     DAOQWT010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42084_length_2661_cov_3.273599"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PKJ95_04585"
     CDS             <1..230
                     /locus_tag="PKJ95_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal L9 C-terminal domain-containing
                     protein"
                     /protein_id="HNR65555.1"
                     /translation="FGSITNKDIAQYLAENYQIEIDRKKIEFNETMKKIGDYPVTIRL
                     HKEVATRLNVIIAPEEETKRDDKVSGEEIKG"
     gene            294..1631
                     /gene="dnaB"
                     /locus_tag="PKJ95_04590"
     CDS             294..1631
                     /gene="dnaB"
                     /locus_tag="PKJ95_04590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNR65556.1"
                     /translation="MELTRSAPQNISAEQATLGSILLEKDSILNCVEILHWEDFYKTA
                     HQLIYQCALDLFEKNQAVDLITLTEELKKRKQLDDVGGASYLTTLMDSVPTAANVIYY
                     ARIVEEKAILRKLINHATQIVTMGYEEQEEARVLLDKAENLIFDVSQQKISTFFSPIK
                     DLLTESFEKIEDLYHSGNFITGVPTGFAKLDELTTGFQPSELIIVAGRPSMGKTAFCM
                     NIAQFAAMQHKIPVAIFSLEMSKSQIVQRMLCSEARIDTHALRRGTMPEEDWPKLSMA
                     AGRLSAAPIFIDDSAGISPLEIKAKARRLKAKYDLGLILIDYLQLIQTGLRVENRQQE
                     ISQISRSLKGLARELNIPVVAVSQLSRAVEQRSNQRPRLSDLRESGALEQDADVVVFI
                     YREEYYKPKSSKKGIAEVTIGKQRNGPTGTLELAFLKEYTRFENLARILEEDE"
     gene            1743..1818
                     /locus_tag="PKJ95_04595"
     tRNA            1743..1818
                     /locus_tag="PKJ95_04595"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1776..1778,aa:Lys,seq:ctt)
     gene            1825..1900
                     /locus_tag="PKJ95_04600"
     tRNA            1825..1900
                     /locus_tag="PKJ95_04600"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1859..1861,aa:Glu,seq:ttc)
     gene            2083..2158
                     /locus_tag="PKJ95_04605"
     tRNA            2083..2158
                     /locus_tag="PKJ95_04605"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2116..2118,aa:Val,seq:cac)
BASE COUNT          910 a          454 c          525 g          772 t
ORIGIN      
        1 tattcgggtc aataaccaat aaggatattg ctcaatattt agcagaaaat taccagatag
       61 agattgatcg caagaagatt gagtttaatg aaacaatgaa aaaaattgga gattacccgg
      121 tgactattcg tctgcacaaa gaagttgcca cccgtttaaa tgtcataata gctcctgagg
      181 aagaaacaaa gagggatgac aaggtctcgg gtgaagagat aaaaggttaa gtaaaataga
      241 ataattagag caagaactat aaaggtcttt taaccagaaa aggagaaaag gagatggaat
      301 taaccagaag tgcgcctcaa aatattagtg cagaacaggc tactttgggt tctattcttc
      361 tggagaaaga ttctatatta aattgtgtag aaatattaca ctgggaagat ttttataaaa
      421 ctgctcacca acttatttat caatgtgctt tggacttatt tgaaaaaaat caggcagtag
      481 atctgattac gctaactgaa gaacttaaaa aaagaaagca attagatgat gtaggggggg
      541 cttcctatct gacaacttta atggacagtg ttcccacagc tgccaatgta atttattatg
      601 ccagaatagt tgaagaaaag gcaattctga gaaaattgat taatcatgct acacaaattg
      661 tgactatggg ttatgaagaa caggaagagg cacgagtgct gcttgataag gcggaaaacc
      721 tgatttttga tgtttctcaa cagaagattt ccactttctt tagtcctatt aaagatttgc
      781 taacagaaag ctttgaaaag attgaagatt tataccatag tggaaatttt attaccgggg
      841 tgcctactgg ttttgcaaaa ttggatgagt tgactaccgg tttccagcca tctgaattaa
      901 tcattgttgc cggaagacca tctatgggga aaaccgcttt ttgtatgaat attgctcaat
      961 ttgcggcaat gcagcataaa ataccagtag ctatcttcag tttagagatg tcaaaatctc
     1021 agattgttca gagaatgctc tgttctgaag ctcgtattga tacccatgct ttacgccggg
     1081 gaacaatgcc ggaagaagat tggccaaagc tgtctatggc tgccggaaga ttatctgctg
     1141 cccccatctt tattgatgat tctgccggta tatccccttt ggaaatcaag gcgaaggcta
     1201 gaagattaaa agctaaatac gatttagggc tcatattgat tgattatctc cagctaatac
     1261 aaactggact aagggtagaa aatcgtcagc aggagatatc ccaaatatcc cgttccttaa
     1321 aagggcttgc ccgggaattg aatattcccg tagtagcagt gtcacagtta tccagagcag
     1381 tggaacagcg ctccaatcaa cgtccccgtc tttcagattt aagagagagt ggagctttgg
     1441 agcaggatgc tgatgtagta gtttttattt accgggaaga atactataaa ccaaaaagca
     1501 gtaaaaaagg aattgccgag gtaaccattg gcaaacaaag aaatggacct accggtactt
     1561 tagaattggc ctttttaaaa gagtataccc gctttgagaa tttagccaga attctggaag
     1621 aagatgagta aaatggataa aggctatttt agtaattttt tttctgaatt tatcgttgac
     1681 actttttata aaatattgta acattgaaaa gcaatcaaaa aaggcatttg agtcatattt
     1741 gcgggccgtt agctcagtag gtagagcatc tgactcttaa tcagagggtc gcaggttcga
     1801 ggcctgcacg gcctaccaat cattgcctcc atcgtctagt ggtccaggac accgcccttt
     1861 cacggcggcg gcaggggttc aaatcccctt ggaggcacca tttattttag aataattgta
     1921 ggcaataagg tttttagttc attgttttaa gtttttaatg aagaataaag aggatatcct
     1981 ttaatgctat gaatgtttga ccaaaaacta agaactataa accaaaaact ttaataccaa
     2041 atactacgac aaacgactaa atactaataa ccattttatt atgggcggat agctcagtcg
     2101 gcagagcacc tgcttcacat gtagggggtc acaggttcaa gtcctgttcc gcctaccatt
     2161 ttttataaaa aatattaaaa ttcaatataa taaaacatct ttcctcaata cgcttccttc
     2221 cctattttcc acaatggata gctctatcct cataatatct tactgatata ttaccgcaat
     2281 tgcgtttaac tcaaaaattg gttaaaaaat atttaagcca gattcttgtt ttttaacgta
     2341 ttgaaaataa taaggtaacg caaactaatg attaatagaa attatctata gataatgtgt
     2401 caaaagtaac tcacaaactc tcgtccgggg cacaatctgg aaaattaacc ataattaaaa
     2461 aaattgactt aatagtcgta gtatgttatt atcataaagt gattattcgt aataatgaaa
     2521 ccatgttcca caatatggaa ttaaggaatt aatcatttta tgaactcagt agaaaaaacc
     2581 attcaaatcc tcaattgcct tagcgataca gacggtaaat taggagttac tgagataagt
     2641 tcaaaattgt ccattcctaa a
//
LOCUS       DAOQWT010000196         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_42136_length_2659_cov_2.903226, whole genome shotgun sequence.
ACCESSION   DAOQWT010000196 DAOQWT010000000
VERSION     DAOQWT010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42136_length_2659_cov_2.903226"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKJ95_04610"
     CDS             <1..182
                     /locus_tag="PKJ95_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65557.1"
                     /translation="LSWDYLSSYLKENNVGTDGVIDVYADKEVDFDYVARIIVVGSEL
                     GIEKINFLLQREYSD"
     gene            complement(276..1301)
                     /gene="amrS"
                     /locus_tag="PKJ95_04615"
     CDS             complement(276..1301)
                     /gene="amrS"
                     /locus_tag="PKJ95_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNR65558.1"
                     /translation="MVQEAMFYKRLENNKVQCLLCSHYCVISPDKRGICRVRENQEGV
                     LNTLNYRKLIAANIDPIEKKPLFHFYPGSQSYSIAAMGCNFRCLHCQNWSISQLSSDK
                     KIGGEFISPERIVQHAQESGCLSISYTYTEPTIYYETALEISQLARQVGLKNIFVTNG
                     YISLDALNHIAPYLDAANIDLKAMSDTFYRKVCGARLKPVLDRIKQYYELRIWIEITT
                     LIIPGYNDGDDELGEIAQFIVDIDKGIPWHISAFYPTYKLNDTPPTPVSTLQRAYKIG
                     KDKGLHYVYEGNVGEGENTLCPSCGKTIISRKYFSTNNKIKNSKCPFCGEKIGGIGMS
                     GENNTNQ"
     gene            complement(1329..2063)
                     /locus_tag="PKJ95_04620"
     CDS             complement(1329..2063)
                     /locus_tag="PKJ95_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNR65559.1"
                     /translation="MKIGIISDIHSNREALEAVLNKLTNVEQFYCLGDIIGYGADPIY
                     CLEKMKEIDCLCIKGNHEGAITGELELYYFNDYAKQSLEWTMKQLREQDFHYIHSLPK
                     KVDITNDILGVHGSPRQPLWEYILDEQTAEGIFNKFDFKVYFIGHSHVPGIFTFHRNN
                     RIIKYFEATAGADLVLDEKHSYIVNCGSVGQPRDGNPQASYVLFDSEKGVINIKRVNY
                     AIERAQRKIIKAHLPHFLAQRLAIGI"
     gene            complement(2200..>2659)
                     /locus_tag="PKJ95_04625"
     CDS             complement(2200..>2659)
                     /locus_tag="PKJ95_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HNR65560.1"
                     /translation="GLMAGCEGDVPSLISMILLHNLTNEPIFLANPVCIDEEKNEITF
                     AHCTIPLNMCESYKLESHFESGMGVAVRGKVEKGPVTIFKLDGNAEKYYLAEGEIVDN
                     LNSPDMCRTQLKISLKIPVHDFLEKSIANHQLICKGWHGPLLEQFFSYLT"
BASE COUNT          816 a          496 c          453 g          894 t
ORIGIN      
        1 atctatcctg ggattatctg tcatcttatt taaaagaaaa taatgtgggt acagacggag
       61 ttatagatgt gtatgctgat aaagaagtag attttgatta tgtagcaaga ataatagttg
      121 ttggaagtga gttgggaatc gaaaagataa attttctttt acaacgagag tattcggatt
      181 aattttttct taaaagatgt gcggaggaga caataatcta atataatttt gtttgttcct
      241 tagatgccat attaattgag ctatgttaca catatctatt gattagtatt attttcaccg
      301 gacattccaa taccacctat tttttctccg cagaaagggc atttgctatt tttaatctta
      361 ttattagtag aaaaatattt cctgctgata attgttttgc cgcaagaggg acaaagtgta
      421 ttttcaccct ctcctacgtt tccttcatat acataatgca gccctttatc tttaccgatt
      481 ttatatgctc tttgcagagt agatactggt gtaggaggag tatcattcaa tttataggtt
      541 ggataaaaag cagaaatgtg ccagggaatt cctttatcaa tatcaacaat aaattgggca
      601 atttctccca gctcatcatc tccgtcattg tatcccggga taatcagagt agttatctca
      661 atccagattc tcagttcata gtattgctta atcctatcta agaccggttt taatctggca
      721 ccacaaacct tccggtaaaa tgtatcgctc attgctttta ggtcaatatt ggctgcatca
      781 agatatggag caatatgatt taaagcatcc agactgatat aaccattggt aacaaaaata
      841 tttttcagtc ccacctgacg ggcaagctga gatatttcaa gggctgtttc gtagtagatg
      901 gttggttctg tatatgtata ggagatagaa aggcatccac tctcctgagc atgctgtact
      961 attctttcag gagagataaa ttcacctcct atcttcttat cactactcag ctgagagata
     1021 gaccagttct gacaatgcaa gcaacgaaag ttacatccca tagcagcaat ggaataagat
     1081 tgagagccag gataaaaatg aaacaagggt ttcttttcaa tagggtctat gtttgcagca
     1141 attaattttc tatagtttag agtatttaaa acaccttcct ggttttctct aactcgacag
     1201 atacctctct tgtctggcga aattacacaa tagtgggaac agagtaaaca ttgcacctta
     1261 ttattctcca gcctcttata aaacattgct tcctgtacca ttaatatcac ctgctattct
     1321 aaatattact aaattcctat tgccaagcgc tgggctaaaa agtgaggaag atgggcttta
     1381 ataatttttc tctgagccct ttctattgca taatttaccc gtttaatatt tataacccct
     1441 ttttcagaat caaacaagac atagctggcc tgaggattac catcacgggg ctggccgaca
     1501 ctaccacaat taacaatata actatgcttt tcatctaaga ccaggtctgc tcctgctgtt
     1561 gcttcaaaat actttattat cctgttatta cggtgaaagg taaaaattcc ggggacatgg
     1621 ctatgtccga taaaatatac tttaaaatca aatttgttaa aaattccctc tgcagtctgt
     1681 tcatccagaa tgtattccca aagaggctgg cggggactgc catgtacacc cagtatatca
     1741 ttggtaatat caactttttt tggtaaactg tggatgtagt gaaagtcctg ttctctaagt
     1801 tgcttcattg tccattcgag agattgtttg gcataatcat taaaatagta tagctctaat
     1861 tctccggtta tggcaccttc atggtttcct tttatacaca agcaatctat ttccttcatc
     1921 ttttcaagac aataaatagg gtcagctcca tagccaataa tatctcccag acaataaaat
     1981 tgctcaacat ttgtcaactt atttaataca gcctctaacg cttctctatt gctatggata
     2041 tctgagataa ttcctatttt caaaactgaa tacccctaaa taaatagtga taagcaatta
     2101 ataattagtt gtttgtgaaa tcccaggttg ttggttataa agtttatagt ttttgatatc
     2161 tttcattttt actatagatt aaaaactatg aacgatatat tacgttaagt aagaaaagaa
     2221 ttgttccagc agaggaccat gccagccctt acagataagc tgatgattgg caatcgattt
     2281 ttctaaaaaa tcgtggacag gtatttttaa agaaatttta agttgtgttc ggcacatatc
     2341 cggggaattt aaattatcaa ctatttctcc ttcagccaga taatattttt ccgcatttcc
     2401 atccagttta aatatagtta ccggaccttt ttctaccttg cctctaacgg caactcccat
     2461 gcctgattca aaatggcttt ccagcttata gctttcacac atattaagtg gaatagtgca
     2521 atgggcaaag gttatttcat tcttttcttc atcaatacat actgggttag ctaagaaaat
     2581 tggttcattg gtaagattat gtagaagtat catagaaatt aaggatggta catcaccttc
     2641 acacccagcc atcaaacct
//
LOCUS       DAOQWT010000197         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_42503_length_2644_cov_2.993820, whole genome shotgun sequence.
ACCESSION   DAOQWT010000197 DAOQWT010000000
VERSION     DAOQWT010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42503_length_2644_cov_2.993820"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..865
                     /locus_tag="PKJ95_04630"
     CDS             158..865
                     /locus_tag="PKJ95_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNR65561.1"
                     /translation="MNSIADRIRTVLREKADKKTLASSQRFFKEPIKCYGVNIQHCTR
                     IARQFWPEVKTLKKDEIYKLCEQLFSSDYSEEAHIVSSWLPEMIELTDENDWYIFQRW
                     IEKYINNWAKCDSFCNHTMGDYLMKFPEFVEELKDWASSENRWVKRAAAVSLIIPARK
                     GYFLNDIFEISDRLMSDKDDMVRKGYGWLLKSASEAYQKEVYEYVLKNREKMPRTALR
                     YAIEKMPEDMRKEAMKR"
     gene            982..2451
                     /locus_tag="PKJ95_04635"
     CDS             982..2451
                     /locus_tag="PKJ95_04635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNR65562.1"
                     /translation="MSDLVKKLKAPKKSKDKKYLEGRNLGEIFLDLIKILPGMVMETD
                     LKGNIRFISKKGIEMLGYTAEELEEKTIWQLASLPGRPNSKSEVQKFLFKNDSPPQEY
                     NLIKKDRSLLPVECYATAIKNEKSKTIGFQSILLDISSRKEYEEKIKYLSFHDKLTGL
                     YNRAYFEEEIQKMDNIRNLPLSIIIGDVNNLKTINDTFGHQHGDQLLIRIAEVLKSCF
                     RRSDVISRWGGDEFSIILPYTTKEKGIEIINRVKQECQRRSTLTLPLSISFGIATKEI
                     DSENINAIVSEAESRMYRYKIFDRQASDSVLIVSMKKALQQKKYDTEEYRQNYIDCAT
                     KFGDTLQLEKTELNKLILLSAICDIGKIAISEEIILKKGWLSGEEWEEIKRHPEIGFR
                     IAKTSPELSNIAEAILYHHEFWDGQGYPQGIRGEDIPLLSRIIHIVDAYQAMTNERPY
                     RKAMDKKEAVEELRRRRGSQFDPHLTQKFIKMITSSEVA"
BASE COUNT          959 a          408 c          549 g          728 t
ORIGIN      
        1 aataaaatgc aaagatgtga gtagaaggga taggaaagag tgtggactac agtgggtgag
       61 attgcttcgt ctgtcagagg cagactcgca aagacggaag ggaggaatac tcacaattat
      121 gacaagagta agatttgata taggggagaa tgaataagtg aacagtatag ctgatagaat
      181 cagaacagta ttaagagaaa aagctgataa aaagacctta gcaagctcac agcgtttttt
      241 caaagaaccg attaaatgtt atggggttaa catacaacat tgtaccagaa ttgccagaca
      301 attctggccc gaggtaaaga cactaaaaaa ggacgaaata tataagttgt gtgaacagct
      361 tttttcttca gattatagtg aggaagcaca tatagtcagt tcctggcttc cagaaatgat
      421 tgaactaact gatgagaatg actggtacat tttccagaga tggattgaga aatatatcaa
      481 taattgggcc aaatgtgatt ccttctgtaa ccataccatg ggtgattatt tgatgaagtt
      541 tccagaattt gttgaagaat taaaggattg ggcttcctcc gaaaaccgct gggtaaaaag
      601 agctgcagca gtatctctga ttattccggc cagaaaaggt tattttctta acgatatttt
      661 tgaaattagc gacagactaa tgtctgataa ggatgatatg gtccgaaagg ggtatgggtg
      721 gctacttaaa tcggccagtg aggcatatca aaaagaggtt tatgagtatg ttctgaagaa
      781 tagagaaaag atgccgcgta ctgcattaag atatgctatt gaaaagatgc ctgaggatat
      841 gagaaaagag gcaatgaaaa gataagaagc attactaaat atattatgtt ataatagttt
      901 tagaaactcg catttaaact tcaagaaaaa gacctgcaat tggctttaat attattttat
      961 taaataaatc agggtaaatt tatgagtgat ttagtaaaaa agctaaaagc tcccaaaaaa
     1021 tctaaagata aaaaatacct tgagggaagg aatttagggg agatatttct tgatttaatc
     1081 aaaatattac cgggtatggt aatggaaaca gatcttaagg gtaatattag atttattagt
     1141 aaaaagggca tcgaaatgct cggttatacg gcggaagaat tagaagagaa gaccatctgg
     1201 caattagcct ccctccccgg gcgcccaaat agtaaaagtg aagtacaaaa atttctattc
     1261 aaaaatgata gcccacccca ggaatataat ttaatcaaaa aagaccgttc cttgctgcca
     1321 gtagaatgct atgctactgc tataaaaaat gaaaaaagta aaaccatcgg ctttcaaagc
     1381 atccttttag acatttcttc acgtaaagaa tatgaagaaa aaatcaaata tctgagtttt
     1441 catgataagc tgaccggtct atataatcgg gcttattttg aagaagagat tcaaaaaatg
     1501 gataatatca ggaatttacc cttgagcata atcattggtg atgtcaacaa cctgaaaacg
     1561 attaatgata cttttggtca tcaacatggt gaccagcttt taatccgaat tgctgaagtt
     1621 ctaaaatcgt gtttcagaag gagtgatgtg atcagtcgct ggggtggtga tgaattcagt
     1681 attattcttc cctataccac caaagagaag ggaattgaaa tcattaatcg ggtaaaacaa
     1741 gaatgccaga gaagaagcac tcttactctt cctcttagca tctctttcgg aatagcaacc
     1801 aaagaaatcg attctgagaa tatcaatgct atagtaagcg aagcagagag caggatgtat
     1861 cgctataaga tatttgacag acaggcttca gatagtgttc ttatagtatc aatgaaaaaa
     1921 gccctacaac aaaaaaaata tgatacagag gaatatcggc agaattatat tgattgtgcc
     1981 actaaatttg gcgatacttt gcaattggaa aagacagaat tgaataaact aatactccta
     2041 tctgctatct gtgatattgg aaaaatagcc ataagtgaag agataattct aaaaaaagga
     2101 tggctgagcg gagaggaatg ggaagaaata aaaagacacc ccgagattgg tttcagaatc
     2161 gccaaaactt ctccggaact gagcaatatt gccgaagcta ttttgtatca tcatgaattc
     2221 tgggatggtc agggttatcc acagggaata aggggagagg atataccact gctctccaga
     2281 attattcaca tagttgatgc ttatcaagcc atgaccaacg agcgtcctta tcgtaaagca
     2341 atggacaaaa aggaggctgt tgaagaattg agaaggaggc ggggaagcca gtttgacccc
     2401 catttaacac agaagtttat taaaatgatt acctcatcag aagtggcata aactataaaa
     2461 ttagtataga ttatattttt tcttttcttt tctcatccca ttgttttcct atcctgtatc
     2521 gtaaaaactc ctttcttgat ttgtgataac agataaataa caatctttgt aatatttgca
     2581 agtattattc gaacctcccc cgctttactg gacaaccaga taagactata atttatacta
     2641 tatg
//
LOCUS       DAOQWT010000198         2642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_42547_length_2642_cov_3.008118, whole genome shotgun sequence.
ACCESSION   DAOQWT010000198 DAOQWT010000000
VERSION     DAOQWT010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42547_length_2642_cov_3.008118"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PKJ95_04640"
     CDS             <1..538
                     /locus_tag="PKJ95_04640"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNR65563.1"
                     /translation="SAYPDSKRGLTYFGKQVVKEMNQLGMIIDVSHISPSGFWSVLKN
                     SSQPIMASHSNCYQICPHRRNLKNEQIKAIAEKGGLICVTYVSDFLIRKKRKATMDDI
                     LTHIDYLVNLVGVDFVGLGSDFDGCRILPEGLEGADKVFTIGEKLTEKGYSREDVNKI
                     MGKNFLRFFQQVTNRNEK"
     gene            668..1198
                     /locus_tag="PKJ95_04645"
     CDS             668..1198
                     /locus_tag="PKJ95_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HNR65564.1"
                     /translation="MNISTRNIAQVSLLIAMSIVLSRMASIRIAVGGVEGIRIGFGKL
                     PIILGSFILGPLYGGLVGAISDFLGYIIQPIGPYLPHFTIISALCGILPVVLFRIMRG
                     NEDHIISIIIPVGITVALTELFLISYSLHIIFAIPWQILLVPRLISVPITIVIYSYLI
                     YVFVRRKVVQIAQHQG"
     gene            1388..1804
                     /locus_tag="PKJ95_04650"
     CDS             1388..1804
                     /locus_tag="PKJ95_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNR65565.1"
                     /translation="MVEFNLEKGLKGVAQVLVGPDNMAERFYHPMPPSFATPMLVSLM
                     DNAAIDAVQKYLPPGYITVATSIIIKHMAPTPEGMTVTAQAELVEVFKNRLTFMVSAY
                     DEVEKVGEGQVQRYIIDLENFSEKVLEKKKKEENPL"
     gene            complement(1980..2288)
                     /locus_tag="PKJ95_04655"
     CDS             complement(1980..2288)
                     /locus_tag="PKJ95_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNR65566.1"
                     /translation="MPTYEYQCKKCNCTFEKFQPITAPPLKYCPKCNGEVYRLISKNG
                     NFILKGSGYYSTDNRKSNLPKERLSSSSDKVSKKDSDNSGCNLKANEGKAKKESASVK
                     "
BASE COUNT          837 a          457 c          495 g          853 t
ORIGIN      
        1 aagtgcttac ccggattcca aaagaggtct cacctacttt ggtaaacaag tagtaaaaga
       61 gatgaatcaa ctggggatga taatagatgt ttctcacatt tctccatcgg gattttggag
      121 tgtattaaaa aactcctctc agcccattat ggcttcccat tccaattgct atcaaatctg
      181 tccacatcga cgtaacctga aaaatgaaca gataaaggca atagctgaaa agggaggcct
      241 tatctgtgtg acctatgttt ccgattttct aatcagaaaa aaaagaaagg ccaccatgga
      301 tgatattctc acccatatcg attatctggt aaacctggtg ggagtcgatt tcgtgggact
      361 gggttctgat tttgacggat gtcggatttt accggaaggt ctggaaggag cggacaaagt
      421 ctttactatt ggagaaaaat taactgaaaa aggctactcc cgggaagatg ttaacaaaat
      481 tatggggaaa aattttctgc gtttcttcca acaggtaacc aacaggaatg agaaataaga
      541 taatggtgat aaacgagggt aatcaaaagc ccgaaacatc acacaaaaag atttctataa
      601 tactgtaaaa tagaaatatt tcactatgtt aaagctgttt atgaaatatg aaaaggatga
      661 aaacattatg aatatctcaa cacgaaatat tgcccaggtc agtttattga ttgctatgag
      721 tatagtactc tcccgtatgg caagtatcag aattgctgtg ggtggagtag agggaatacg
      781 gattggtttt ggaaaattac ccattattct gggaagcttt atacttggtc cgctctacgg
      841 cggcctggtt ggtgctattt cagattttct gggctatatt atccagccca ttgggcccta
      901 tcttccacat ttcaccatta tttccgctct atgtggtatt ctaccagttg ttctgttccg
      961 tattatgaga ggcaatgaag accatatcat aagtattatt atcccggtgg gaataactgt
     1021 tgcccttacc gagttatttt taatatctta cagtcttcat attatctttg ctattccctg
     1081 gcaaatcctc ttagttccca gattgataag tgtacccatt actatagtca tttattctta
     1141 tcttatttat gtatttgtcc ggcggaaagt ggtacagatt gcccaacatc aaggataaca
     1201 tcccggctga tttgcttatt tatcgctttg tcaatagggt aaaagtttaa gaataattat
     1261 tcccacttta attttgttta atcgaaagta aaatctttga tataataaat atagaatgaa
     1321 tgtataaatc aggaggcttt ccgaaaagtt acacctttat attttataaa atagtaagga
     1381 ggtcaaaatg gtagaattta atttagaaaa aggtctgaaa ggtgttgctc aggtgttggt
     1441 tggcccggat aatatggcag aaaggtttta tcaccctatg cccccctcat ttgctacacc
     1501 tatgctcgtt tctctgatgg acaatgccgc aattgatgca gtgcaaaaat atttaccccc
     1561 ggggtatatc actgtggcaa cttcaataat tatcaagcat atggctccta cacctgaggg
     1621 aatgacggtt actgctcagg cagagctggt agaggttttt aaaaatcgtc ttacctttat
     1681 ggtatctgct tatgatgagg tagaaaaggt aggagagggt caggtacaac gctacataat
     1741 tgatttagaa aacttttcag agaaagtatt agaaaaaaag aaaaaagaag aaaatcctct
     1801 ttagtgcatt tgtaaaaagc aataccagaa tagattttcc atcttgaata tgttctttgt
     1861 taaaaatagg gagagatatt catataaaaa aaccacaccg ggtaaaggtg tggtttttat
     1921 gtatttaatc atcctttaat tttattaaag aatggagccg gaatacaggc tcaagttttt
     1981 tatttcacac tggcagattc ttttttagct ttcccctcat tagctttcag attgcaaccg
     2041 gaattatccg aatctttctt acttacctta tcactactac tgctcaatct ctctttaggc
     2101 aggttactct ttctattatc tgtgctataa tagccgcttc cttttaaaat gaaattacca
     2161 tttttactga ttaaccgata tacttcacca ttacatttag ggcaatactt taagggtggt
     2221 gcagtaatag gttgaaactt ttcaaaagta caattacatt tcttacattg atattcatag
     2281 gtcggcatat ttcttttcag ctccttcctt atcattccaa tgaaaaataa tattgcattt
     2341 taacatattt gtcaagatat tttcaacaag ttactggcat aaaacctgaa tatccattga
     2401 attgactaaa tagaataaat tatctataat aaaagaacaa gaactggttg gtataaaaaa
     2461 atataaaaat ataaggaggt cttttcaatg gaaaagaaaa aaatggggtt ggcggcaagg
     2521 gttcttatag cattagctat tggtattata gttggtataa tagttcattc tcttcccccc
     2581 ggcgcactgc gaacactatg accttgtcaa gtacttttta cgtacttttt acatacatag
     2641 ga
//
LOCUS       DAOQWT010000199         2641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_42573_length_2641_cov_2.531323, whole genome shotgun sequence.
ACCESSION   DAOQWT010000199 DAOQWT010000000
VERSION     DAOQWT010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42573_length_2641_cov_2.531323"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKJ95_04660"
     CDS             <1..246
                     /locus_tag="PKJ95_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNR65567.1"
                     /translation="EIEERIDNQLTMLLADTYTIPVNLAGLPALSINCGFTSKERLPV
                     GLQIIGGPFQEEKMLRVAYNLEQELKEINTVEPNLLF"
     gene            328..1779
                     /gene="gatB"
                     /locus_tag="PKJ95_04665"
     CDS             328..1779
                     /gene="gatB"
                     /locus_tag="PKJ95_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNR65568.1"
                     /translation="MESNTVIGLEIHIQLLTDSKIFCNCSTDYIDREPNSHTCPVCLG
                     LPGALPVLNKKVVELALKTALALNCHINRTNIFHRKHYFYPDLPKGYQISQHDQPLAV
                     NGFLEIEMEEPLENKKIRINRIHMEEDAGKLIHLEKDGAAVCSLVDYNRSGIPLLEIV
                     TEPDIQSPQEAIVFLQKLRNIVQYLGVCDGNLESGSMRCDANISIREENSGRLGTKTE
                     IKNMNSFKAIKEALDYEVMRQRKLIEQGDKVSQETRRWDEESGETVFMRSKERAQDYR
                     YFPEPDLLPLTIEQSWIDGIKKNIPELPAERKERFMVDYHLSGYDANRLVEVKPLGDY
                     FEKAAKSYNNYKKLANWILGELLHYLGEENIGMEKSPVSPSALVGLLKLIDQDVISVK
                     IAKNIFEQMFKTGQSAAVLVQNSGFTQISNEKEIDGIIRIVLRDNPETVQDFASGKEK
                     AIHFLVGQVMRHTKGRAQPDMVLNLIKERITEI"
     gene            1864..2025
                     /locus_tag="PKJ95_04670"
     CDS             1864..2025
                     /locus_tag="PKJ95_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNR65569.1"
                     /translation="MERYICTVCGYIYDPEKGDPDSGIEPGTSFDDLPDDWVCPLCGA
                     GKDEFEKEE"
     gene            2028..>2641
                     /locus_tag="PKJ95_04675"
     CDS             2028..>2641
                     /locus_tag="PKJ95_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNR65570.1"
                     /translation="MAVKEISKNVYSIGIIDWNRRLFDELIPLPDGTSYNSYLLKNQK
                     EALLIDTADPSKMEEFLQNIEESEVEKISYIIAQHAEQDHSGSIGKVLDRFPGCKVIA
                     NRKCLDLLKDELHLNEEDFIEIKDNEIFHFADFEIQFIFAPWVHWPETFISYLPKEKI
                     LFTCDLFGSHLASSELWSQDNIKNYLAAKRYYAEIMMPFRNNIQ"
BASE COUNT          888 a          404 c          578 g          771 t
ORIGIN      
        1 gaaatagagg aaagaataga taaccagttg accatgcttt tagcagatac ttacaccata
       61 ccggttaatc tggctggttt acctgcttta tcaattaatt gtggctttac ctcaaaggaa
      121 aggttaccag ttggacttca gattatagga ggccctttcc aggaagaaaa gatgttgagg
      181 gttgcttata atttagagca agaattaaaa gaaatcaaca cagtcgaacc aaatttatta
      241 ttttaacaaa ttctgaaact ataaaaacaa taaatagttt tttataaccc cagtatattt
      301 cagtaaagga gaaaatagga gttgtcaatg gaaagtaata cggtaattgg attagagata
      361 catattcagc ttttaactga tagcaaaata ttctgtaatt gtagtacaga ttatattgac
      421 cgggaaccta attcccatac ctgtccggta tgtcttggtt taccgggggc ccttccggta
      481 ctcaacaaaa aagtagtgga attagcttta aaaactgccc ttgctcttaa ttgtcatatc
      541 aacagaacta atattttcca tcggaaacac tatttctatc ctgatttgcc aaaaggatat
      601 caaatatccc agcatgacca gcctttagca gtaaatggtt ttttggaaat agagatggaa
      661 gaaccattag aaaataaaaa gataagaatt aatagaatac atatggaaga ggatgccggc
      721 aagctgattc atctggagaa ggatggtgca gcagtctgtt cgctggttga ttataaccgt
      781 tccggtattc ctctcttgga gatagtaaca gaacctgata ttcagtctcc ccaggaagca
      841 atagtttttc tacagaaatt gagaaatata gtacaatatc tgggtgtttg tgatgggaat
      901 ttagagagcg gttctatgcg ttgtgatgcc aatatttcta tcagggaaga aaatagtggc
      961 aggctgggaa caaaaactga gataaaaaat atgaattcat ttaaagctat aaaagaagct
     1021 cttgattatg aagtaatgcg acaaagaaaa ttgattgaac agggagacaa ggtatcacag
     1081 gagaccagac gctgggatga ggaatctggt gagactgtct ttatgagaag taaagaaagg
     1141 gctcaggatt acaggtactt tcctgaacca gacttgttac ctttgactat tgaacagagt
     1201 tggattgatg gtattaagaa aaatattcct gaattaccgg cagagcgaaa ggaaaggttt
     1261 atggtagatt accacctttc tggctacgat gccaatcgcc tggtagaagt aaaaccatta
     1321 ggtgattatt ttgaaaaagc cgctaaaagc tataataatt ataagaaatt agctaactgg
     1381 attctgggag aattattgca ttatcttggt gaggaaaata taggaatgga gaaatctcca
     1441 gtatctccct ccgcattagt cggattgtta aaattaattg accaggatgt tatcagtgta
     1501 aagattgcca aaaatatctt tgagcagatg tttaagacag gtcaaagtgc cgccgtttta
     1561 gttcaaaata gcggctttac tcaaattagc aatgaaaagg agattgatgg tataattaga
     1621 atagttctca gggataatcc tgagacagtg caggattttg cttctgggaa agagaaggca
     1681 atccattttt tagtgggaca ggttatgagg catacaaagg gaagggcaca gccggatatg
     1741 gtcttgaatc tgatcaaaga aagaattacc gaaatctgaa tttagcagaa tagctgaggt
     1801 ggtaaacttc ttctatagaa agacagaaaa ttaaatcaat aaacgaggag aggaggtgtg
     1861 tctatggaac gatacatctg tacagtttgt ggctatattt atgacccgga aaaaggggac
     1921 cctgactctg gcattgagcc gggaacatct tttgacgatt tacccgatga ttgggtttgt
     1981 cctttatgtg gtgctggtaa agatgaattt gaaaaggagg aataattttg gctgtaaaag
     2041 aaatttctaa aaatgtttat tctattggga ttattgattg gaatagaagg ctttttgatg
     2101 aactcattcc cttgcccgat ggaaccagtt ataattccta tctactaaag aatcagaaag
     2161 aagcattatt gatagatact gctgacccct ctaaaatgga ggaatttctt cagaatattg
     2221 aagaatcaga ggtagagaag ataagctata ttattgccca gcatgccgaa caggaccatt
     2281 ctggctctat agggaaggta ttggacaggt ttccgggttg taaggttatt gccaatcgaa
     2341 aatgtttgga cttgttaaaa gatgaattgc atttaaatga agaagatttt attgaaatta
     2401 aagataatga aatatttcat tttgccgatt ttgaaattca gtttatcttt gcgccatggg
     2461 tgcattggcc cgagaccttt atatcctatc ttccaaaaga aaaaatccta tttacctgcg
     2521 acctgtttgg ttcacacctt gctagcagtg aattgtggtc ccaggacaat attaaaaatt
     2581 acctggctgc taagcgttat tacgctgaga ttatgatgcc ctttcggaac aatattcaga
     2641 a
//
LOCUS       DAOQWT010000200         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_42710_length_2635_cov_2.767829, whole genome shotgun sequence.
ACCESSION   DAOQWT010000200 DAOQWT010000000
VERSION     DAOQWT010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42710_length_2635_cov_2.767829"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..1554
                     /gene="secD"
                     /locus_tag="PKJ95_04680"
     CDS             364..1554
                     /gene="secD"
                     /locus_tag="PKJ95_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNR65571.1"
                     /translation="MKQLKIFRLALVIIVIVIALYYVFPLDKNINLGLDLQGGSHIVL
                     ECIDSPNAPVDSDAVNRVIEIISNRINPEGVKEPVIQRQGDRRILVQLPGMDDPQEAE
                     SLIGKTALLEFKDQNGETLLTGAYLKNAKTSFDRFGRPNVILEFDEEGAKLFEQATTK
                     NVGKILAITLDGQEISSPVVQEPIPSGEASIVGQFTVEEAQRLALLLRSGALPVEVKI
                     LENRSIGPTLGRDSINRSLKAGLVGLALILIFMIAIYKWFGLVADLALLVCMLLIMGG
                     LAILNATLTLPGIAGIILTIGMAVDANILIFERIKEELKMDKTFRASIEAGFSKAFVA
                     IFDANVTTLIAAVALLYFGSGPIRGFAVTLSIGIVVSMFTALVVTKLILELLGQRLTK
                     RALV"
     gene            1572..2540
                     /gene="secF"
                     /locus_tag="PKJ95_04685"
     CDS             1572..2540
                     /gene="secF"
                     /locus_tag="PKJ95_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNR65572.1"
                     /translation="MMNILSNTDIKFVSKRKLFYIITGIAILIGIFSLLFQGFNFSID
                     FAGGTLIQLAFKEGNVSVAEIRDVLREINLAGSTIQSLSNNEFIIRTAQVELQERQNI
                     LKTIEDRLGSFEILRVETVGPVIGQSLKQLASLAVIFAFIGMIVYITIRFQFKYAIAS
                     IIGLAVAVLIVLGILSILQKEISIPIIAAILTIVGYSINNTIVIFDRLRENLKTKLRL
                     PLEEVIDLSINQSLSRTINTSLTTLLPVLALYFFGGTLSDFAFVLLVGITVGTYSSIF
                     IAGPILMEFNKKRRISDKKIPTSTQKVVEPKPKVSPSKVSPRLLKK"
BASE COUNT          859 a          377 c          540 g          859 t
ORIGIN      
        1 aaagcgaaag ggaaaactta agggcaggtt tttaaccttc ctttcttctt attctggtta
       61 tttattaaga atgataaagc taaatagatt gacagtagaa tttatttaat aatataatca
      121 tatggttttt aatttatctg ctttacttct tttgttttaa aatttaaatt cctggtaaca
      181 acatctataa aaaatgttta acaataaaaa atgtaaaaca aataaattat aaaaaacgat
      241 gattaagata ttattcgtca agaacagagt aaaggaaaga tcgaaatttg agaacgatac
      301 ttaaatttgg aaatcaataa ttggaggggg tagtaaccct cttatattca gaggagtaat
      361 aatatgaagc agttaaaaat atttagatta gcccttgtta ttatagttat tgtaatagct
      421 ctttattatg tctttccctt ggataagaat ataaatctgg gtctggactt gcaaggtggt
      481 tcacatattg ttttagaatg tattgattca cctaatgcac cggtggatag tgatgctgtg
      541 aaccgggtga ttgaaatcat atctaataga atcaaccccg aaggagtgaa agaaccggtt
      601 atacaacgcc agggagatag aagaatattg gtgcagttac cggggatgga cgatcctcag
      661 gaggcggaaa gtctcattgg aaagaccgct ttgttggaat ttaaagacca gaatggcgaa
      721 acgttattga ccggggctta tttaaaaaat gctaaaactt cttttgatcg ttttggcagg
      781 cctaacgtta ttctggaatt tgatgaggag ggagcaaaac tatttgaaca ggcaacgact
      841 aagaatgtag gaaaaattct ggcaattact ttagatggtc aggaaatatc ctcaccggtg
      901 gtacaggagc caattcctag cggagaagcc tctattgtgg ggcagtttac tgtagaggaa
      961 gcacaaagat tagccctgct gttaagaagc ggtgcccttc cggtagaggt taaaatatta
     1021 gaaaatcgtt ctatcggacc cactctgggc agagattcca ttaatcgcag cttaaaagca
     1081 ggtctggttg gattagcttt gatactcatt tttatgatag ctatttataa atggtttggg
     1141 ctggttgcag atttggcatt attagtatgc atgcttttaa taatgggagg tcttgctata
     1201 ttaaatgcaa cactaacttt acccggaata gcgggtatta tacttaccat aggaatggct
     1261 gttgatgcca atatcttaat ctttgaaaga attaaggaag aattgaaaat ggataaaacc
     1321 tttagagcat ccattgaagc tggattttcc aaagcatttg tagcaatttt tgatgccaat
     1381 gttaccactt taattgcagc tgttgccctg ctttatttcg gtagcggtcc tatcagggga
     1441 tttgctgtga ctttaagtat tggtattgtg gttagcatgt ttaccgctct ggtggttact
     1501 aaattgatat tagaattatt aggacagcgc ttgaccaaga gggcgttggt ttaacatttt
     1561 attggaggat tatgatgaac attcttagta atacagatat taaatttgtt tcaaaaagaa
     1621 aattgtttta tataattact ggtatagcaa tcctgatagg catattctct ctattgtttc
     1681 aaggatttaa ttttagtatt gattttgctg gaggcacatt aatccaatta gcgtttaagg
     1741 aagggaatgt atctgttgcc gaaattagag atgtgttaag agagataaat ttagcaggca
     1801 gtactattca atcattatcc aataatgagt ttataataag aactgcccag gttgagcttc
     1861 aggaaagaca gaacatatta aaaacaattg aagataggtt aggttctttt gagatattga
     1921 gagtagaaac agttggaccg gtaattggtc aatctttaaa acagctggcc agtcttgctg
     1981 ttatttttgc ttttattggt atgatagttt atattaccat aagatttcag tttaagtatg
     2041 ccattgcttc tattatagga ttggcagttg ctgttttgat tgtcttagga atcctatcta
     2101 tattgcaaaa ggaaataagt attcccatta tagccgctat cctgactatt gttggttatt
     2161 ccataaacaa tactattgta atatttgaca ggctgagaga aaatttaaaa acaaagttgc
     2221 gtttgcctct ggaagaagtc attgatttaa gtataaatca atcattaagc agaactatca
     2281 atacctcact aactacttta ttaccggttc tggctctcta tttttttggt ggcacactat
     2341 ccgattttgc ctttgtactg ttagtaggca tcactgtagg aacttattct tcaatcttta
     2401 tcgcaggtcc tatacttatg gagtttaata aaaaaaggag aatatcagat aagaagattc
     2461 ctaccagtac tcaaaaagtt gtagagccaa agcctaaggt tagcccaagc aaggtcagcc
     2521 caaggttgtt aaaaaagtga agagtaaaaa gaagaaataa atttaatata ataaatcata
     2581 ttaactgacc agctctgtta tcaggaggat aaaaccgaat ggatttaagg aatgt
//
LOCUS       DAOQWT010000201         2630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_42823_length_2630_cov_3.053592, whole genome shotgun sequence.
ACCESSION   DAOQWT010000201 DAOQWT010000000
VERSION     DAOQWT010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42823_length_2630_cov_3.053592"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKJ95_04690"
     CDS             <1..291
                     /locus_tag="PKJ95_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNR65573.1"
                     /translation="YSGEKGDIHVTVFENNYIVSETIYEAAMTYLIRQNGKLKIYNEV
                     HTLGLYPYNTWQNIFKENNLKISQVDMNHLYDQYLLEDGQYKLKIFSGILKK"
     gene            360..1331
                     /locus_tag="PKJ95_04695"
     CDS             360..1331
                     /locus_tag="PKJ95_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifH"
                     /protein_id="HNR65574.1"
                     /translation="MINWKSYLKANIISWLLEEDNPSVRYFTLIDLLEKPKYNLELIQ
                     TRKDILQRGIVPVIFSRQQEEGYWGEEEKYYTNKYRGTVWQLLILAESGIEKEDDARV
                     EKACKFILEHAQDKKRYGFSVYHSAKAGGGRSNSVIPCLTGNMVFSLIRLGFYADERV
                     QKAIQWIITYQRFDDGNYRLPDDWPYDKSDLGCWGKHSCHMGVVKTLKALAEIPINLK
                     NEEINRTIEIAVDYLLKHHIYKKSHDLKKIAKPGWLRLGFPLMYQTDILEILEILTKL
                     GYRDNRMQDAIDILISKQDNQGKWIMENTFNDRFLVNIEKKESLVNG"
     gene            1427..1855
                     /locus_tag="PKJ95_04700"
     CDS             1427..1855
                     /locus_tag="PKJ95_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65575.1"
                     /translation="MLTEEQHRKIIKWASICSKHVLSLVKGNIDQRLTLALFVAKEWE
                     EGKIATGEARKASVNAHTIARESSDPVARAVARSVGHAVATAHMADHSIGAALYARRA
                     IKFTGKSEDTERQWQIEQIPREIKSIIITMMEEKEKHFKT"
     gene            2268..>2630
                     /locus_tag="PKJ95_04705"
     CDS             2268..>2630
                     /locus_tag="PKJ95_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65576.1"
                     /translation="MKRMFVLSVLLIIFFVSGCANNIPLLTLSNVEWYTTTETIGKLT
                     FGYVHLSLSGATDGERVTVMTYGDGLISEEELILTDDKKFNQDIIIKFTHSADSEPRV
                     YSTVVTAYNGTIQKKIDLV"
BASE COUNT          996 a          346 c          506 g          782 t
ORIGIN      
        1 tattctggtg aaaagggcga tattcatgtt acggtatttg aaaataatta tattgtgtca
       61 gaaacaatct atgaggcagc aatgacttac ctgattcgtc agaatggtaa actgaaaatt
      121 tacaacgagg tccataccct tggactgtat ccttataaca cctggcagaa tatttttaaa
      181 gaaaataact tgaagataag ccaggtagat atgaatcatc tttatgacca atatctgtta
      241 gaagatggtc agtataaatt gaaaatattt tccggcattc tgaagaagta aattagagtc
      301 aataaaacac tcatcaagaa ataatattga aaatgttcta atttattgaa ggattaatta
      361 tgattaactg gaaatcttat ttaaaggcaa atataatttc ctggctttta gaagaagata
      421 atccatcagt acggtatttt actttgatag atttattgga aaagcctaaa tataatcttg
      481 agctgattca aacaagaaaa gacatactac aaagaggtat agttcctgtt attttttcta
      541 gacaacaaga agaaggatat tggggagaag aagaaaaata ctataccaat aaatacaggg
      601 gaacagtttg gcaattattg attctggcag agtcaggaat agaaaaagaa gatgatgcta
      661 gggtggaaaa agcctgcaag tttatcctgg aacatgctca ggataaaaag cgttatggat
      721 tttcagtcta tcatagtgct aaagccggag gaggcagatc caatagtgtg ataccatgcc
      781 ttaccggtaa tatggttttt agccttatta ggttaggttt ttatgctgat gaaagagttc
      841 aaaaagcaat tcagtggata ataacctatc aacgctttga tgatggaaat taccggttac
      901 cggatgattg gccttatgat aagtcagatc tgggctgctg gggaaaacat tcttgtcata
      961 tgggagtcgt gaagacattg aaagctttgg cagaaattcc tattaattta aagaatgagg
     1021 aaataaatag aacaattgaa atagcagtag attatttact taaacaccat atttacaaga
     1081 aaagccacga tttgaagaaa atagcaaaac cagggtggct tcgcctggga tttcccctaa
     1141 tgtatcagac tgatattctt gagattttag aaatattaac taaattaggt tatcgagata
     1201 acagaatgca ggatgccata gatatcctga tttccaaaca ggataaccag ggaaagtgga
     1261 taatggaaaa tacctttaac gaccgctttc tggtaaatat tgaaaaaaaa gaaagcctag
     1321 taaatggttg accttaaagg ctttgacagt gttgaaaaga tattttatgt gagtattaag
     1381 gaattatatg cgggataata aatttattac agttgatcga ggagggatgc ttactgaaga
     1441 acagcatcga aaaataatta aatgggctag catatgttcg aaacatgtat tatcacttgt
     1501 taaaggaaat attgatcaga gactgacatt ggctttattt gttgccaaag aatgggaaga
     1561 aggtaaaata gcaacaggtg aggctagaaa agcttccgta aatgcgcata ccatagctcg
     1621 ggaatcttct gatccggttg caagagcagt ggcccgttct gtcggacatg cagtggcaac
     1681 tgcacatatg gctgaccatt caataggggc agcattatat gctagaagag ctataaaatt
     1741 cacaggcaaa tcagaagata ctgagcgcca atggcaaatt gaacaaatac caagggaaat
     1801 aaaaagtatt attattacaa tgatggaaga aaaagagaag cattttaaaa catgaataat
     1861 cttcagaaat taaattgaaa tgttaagaaa atatatcata gatgtcgata atattctatc
     1921 attagattta taaaaaaata ctcatcacag attgtattta ttgattctgt attacaacct
     1981 tccaaacaaa tatcaaatat aactctatta tttagctcaa aagatgaaga aaaaaataat
     2041 gcaacggttt tgaaaaaata tttagaaaca ttagagtatg ataattaacc agagattagt
     2101 gatttaaaga tattaattaa attggaacaa gatactcttt aagtaattac tcgattatat
     2161 tcaataaata agagtacatt aatgttaaaa attgtcatct aaagaaaata tgtatatata
     2221 ttcaagaaaa taaaaataat tttagttaaa acaaaggatt caaaaatatg aagagaatgt
     2281 ttgtgttatc agtattatta attatttttt ttgtttcagg gtgtgctaat aacataccat
     2341 tactaacact ttctaatgtc gaatggtaca ctactactga aactattggc aaattgacct
     2401 ttgggtatgt tcaccttagc ttgagtggag ctacagatgg agaaagagtt actgtaatga
     2461 cctatggaga tggattaata tcagaggaag agttaatatt aactgatgat aaaaaattta
     2521 atcaggatat tattattaag tttactcact cagctgatag tgaacccagg gtttatagca
     2581 ctgtagtcac tgcttataat ggtactattc agaaaaagat tgaccttgta
//
LOCUS       DAOQWT010000202         2630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_42829_length_2630_cov_2.800777, whole genome shotgun sequence.
ACCESSION   DAOQWT010000202 DAOQWT010000000
VERSION     DAOQWT010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42829_length_2630_cov_2.800777"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PKJ95_04710"
     CDS             complement(<1..1233)
                     /locus_tag="PKJ95_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNR65577.1"
                     /translation="MEKNETKPKDYHKDIQIGGMTCINCANRVERGLKKIEGVKFASV
                     NLATETAFIVSEREISAQEIKEAVQWAGYSVLEGDRGDLSQVRYKESKRRLIMAWIVT
                     APLSLLMILHMLGYHISGFVLIEIISVAFLAFYCGMATIKSAWIAFRHFHSNMDTLIT
                     IGTVTSWLTAVLNYSGLPIISFGTIGGMIITIHLTGRFIESNLRDKAAKEIKALIALQ
                     AKEATVILENEEIKVPITMLKKDQLIKVKPGERVPVDGIVIDGASAVDESFISGEPIP
                     RKKEKGDEVVGGSINLSGLLIVKAVKIGEESFLSQMITLIKEAQGAKVPIQALADNIT
                     NIFVPVIITLATLSALFWFFNLNNYQPFLLKAKEIFPWILITENKYSFAVFVFISTVV
                     IACPCALGLATPMALVVGS"
     gene            complement(1361..2239)
                     /locus_tag="PKJ95_04715"
     CDS             complement(1361..2239)
                     /locus_tag="PKJ95_04715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HNR65578.1"
                     /translation="MLESIRRKIESAEDLYSVVKTMKALSAVNIHVYERAVQAINKYF
                     QSIENGLQIVLKKNPEMLITNNKISEGVTGIIAFGAQKGMAGNFNLPVVNKLKGWVEK
                     KRADLKFSPVVCSIGDRIREQLKDAGFSPESSFDFPAFRYHIDSTIQDLLLVIESWRF
                     QKNVNTIHLFYNKPIRGVSFQPITLQLFPLDISWLRSVAKKSWPSRSIPLYTLTTGEL
                     FNHLIRQYFFIILFRAFIESMASENASRLMTMQKAEQNIEERLENLNAQFNHQRQKAI
                     TDELLDIIAGFEALTK"
     gene            complement(2248..>2630)
                     /locus_tag="PKJ95_04720"
     CDS             complement(2248..>2630)
                     /locus_tag="PKJ95_04720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012527.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNR65579.1"
                     /translation="VGDLRLSYSQFQELEIFSRFGTQLDENTEKVLIRGRRIREILKQ
                     NQYHPYSVLEQIIYLLAISHGLLDGIEESAVSEFKRLIREKKEDRLVGIEERINNNEL
                     LSPQDEDILIDYVKEIIQSMVQGQ"
BASE COUNT          777 a          566 c          454 g          833 t
ORIGIN      
        1 gcttcccact accagagcca ttggagtagc taaacctaag gcacaaggac aggcgattac
       61 cacagtggaa ataaagacaa agaccgcaaa agagtatttg ttttcagtta ttaaaatcca
      121 gggaaatatt tcttttgctt ttaataagaa tggttgatag ttgttcagat tgaaaaacca
      181 gaacaaggca cttaaggtag caagggtgat aataacagga acaaagatat tagtgatatt
      241 atctgccaga gcctgaatgg gcaccttggc gccctgggct tcttttatta aagttatcat
      301 ctgggataag aaggattctt cccctatctt aactgcctta acaatcaaga gcccggaaag
      361 attaatagat cctccgacta cctcatcacc tttctccttt ttcctgggga tcggctctcc
      421 ggagataaaa gattcatcta ccgctgaggc accatctatg acgattccgt ctacaggaac
      481 ccgttcgccg ggcttaacct tgattagctg atcctttttc agcatagtta taggcacttt
      541 aatttcttca ttttctaaaa tgacagtagc ttctttggcc tgtaaagcga tcagggcttt
      601 tatttctttg gccgctttat cccttaaatt tgattctata aaacgaccgg ttagatgtat
      661 agtgattatc attccaccaa tagttccgaa cgatataatc ggcaatcctg aatagttcaa
      721 aactgcggtc agccaggagg ttactgttcc aatagtaata agagtatcca tgttggaatg
      781 gaaatgccgg aaggcaatcc aggcactctt tatggtagcc ataccgcaat agaaggcaag
      841 aaaggcaaca gagataattt caatcagtac aaagccggag atatgataac ccagcatgtg
      901 caaaatcatc aatagagaga ggggagctgt tactatccag gccataatca atcttctttt
      961 tgattcttta taccgaacct ggctcagatc tcctctgtct ccttccagta cactataacc
     1021 agcccattgt accgcttctt ttatttcctg ggctgaaatt tcccgttcac tgactataaa
     1081 ggcggtttcg gttgccaaat tgacagaggc aaattttacc ccctctattt ttttgagacc
     1141 tctttccacc ctgttggcac aattgatgca ggtcatccca ccaatctgaa tatctttatg
     1201 gtaatctttg ggtttggttt cattcttttc catttaatat aacctatata ctgttatctt
     1261 tgaaagtgca ggttttcttc ttcaatttat atactatacc acattttaaa aacataattt
     1321 ctatagtttt aatctattta tagaataaaa tgaaaatgat tcatttggta agtgcctcaa
     1381 atccggcaat aatatccagt aattcatcgg tgatggcttt ctggcgctga tgattgaatt
     1441 gggcatttaa attttccaga cgctcttcaa tattttgctc tgctttttgc atagtcataa
     1501 ggcggctggc attctcgcta gccattgatt caataaatgc ccggaatagt ataataaaaa
     1561 aatattgtct gataagatgg ttaaacagtt ctcctgtggt cagagtatag aggggaatgg
     1621 aacgtgaagg ccaacttttt tttgccacac tcctcagcca acttatatcc agaggaaaga
     1681 gctgcagagt tattggctgg aaagaaaccc ctcttatggg cttattgtag aaaagatgta
     1741 tggtattaac atttttctgg aaacgccagc tctcaatcac caacaaaagg tcctgaatag
     1801 tactatcaat atgataacga aaagccggga aatcaaaact actttccgga gaaaagccgg
     1861 catccttcaa ttgttctctt attctatccc cgattgaaca gactaccgga gagaatttta
     1921 agtcagctct ttttttctca acccagccct tcaacttatt gacaaccggt aaattaaaat
     1981 tacctgccat acccttttgg gctccaaaag ctatgatacc ggtaactcct tcactgatct
     2041 tgttgtttgt aataagcatc tccggatttt tctttaagac aatctgaagg ccgttctcaa
     2101 tactttgaaa atatttatta atcgcctgta ctgccctctc atagacatgg atattcaccg
     2161 ctgaaagtgc cttcatagtc ttaacaactg agtaaaggtc ctcagcactt tcaatttttc
     2221 gtctaatact ctccagcatc tgcataatta ttgcccttga accatagact gaataatttc
     2281 tttaacataa tctatcagta tatcttcatc ctgtggggat aataattcat tattattaat
     2341 ccgttcctca atcccgacca accggtcttc cttcttttct cttatcaatc ttttaaactc
     2401 tgaaaccgca gattcctcta tcccatccag caaaccatga gaaatagcca gtaaatagat
     2461 aatctgttct aaaactgaat aggggtgata ttggttttgt tttaaaatct ctctgattct
     2521 tctccccctt atcaatacct tttcagtatt ttcatctaat tgagtgccaa agcgagaaaa
     2581 aatttctaat tcctgaaatt gagaataact cagccttaaa tcacctacta
//
LOCUS       DAOQWT010000203         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_42929_length_2626_cov_2.287048, whole genome shotgun sequence.
ACCESSION   DAOQWT010000203 DAOQWT010000000
VERSION     DAOQWT010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42929_length_2626_cov_2.287048"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PKJ95_04725"
     CDS             <1..795
                     /locus_tag="PKJ95_04725"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HNR65580.1"
                     /translation="KEIENSEQYRKKGELIMANLWNIKRGIDQVSLVDYSLPNHPRVE
                     IALNPDLSPLQNAHQLFQRYKKLNPGKERNREQLLENQKSLKQLKDMFSTLYESKNSL
                     SELSALYQELTVPNYIKKKKVGNGKHLKGRVPSIQKLISSDGWTILVGRNNRQNEYLL
                     RHLSNGNDFWLHNQTRPGAHVLIKNHQGLPVPPPDTLIFAARLTGYYSKVKNNETAQI
                     VYTFRKYVKKPKDSIAGKVIYSQEKNIAVVINYEEIKKEINKLIVV"
     gene            complement(792..1739)
                     /locus_tag="PKJ95_04730"
     CDS             complement(792..1739)
                     /locus_tag="PKJ95_04730"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNR65581.1"
                     /translation="MNSKNSQKFVHNIQFQEKERVRVDIFLAKENIAGLSRNRIKELI
                     NQGHITVNGREIKPSHILKSGDNISLAIPDNRELVLQPENIPLNIYYEDEHMMVIDKA
                     AGMIVHPTGKVHSGTLVNALLFHCQGQLSGISGINRPGIVHRLDKETSGLMMIAKTEL
                     AHRSLSIQIKERKVIKKYIALVHNLVKDEEGWIDAPIGRSPQHGNKMTVTNIGGREAK
                     TYFKVIKYFASHTLLILDLHTGRTHQVRVHLSYLGYPVVGDKVYGSFKRDDPSLIIDR
                     QALHSHFLEIVHPVKQQSLTFTSPLPDDFLEQLKMLATP"
     gene            complement(1799..2272)
                     /gene="lspA"
                     /locus_tag="PKJ95_04735"
     CDS             complement(1799..2272)
                     /gene="lspA"
                     /locus_tag="PKJ95_04735"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNR65582.1"
                     /translation="MNFIIGVLLVILTDQVSKVWVSSTMELGESIPIINNIFHITYIQ
                     NPFSSFGLLKFSNKTFIITGLVIILIGIILLLKIARNNKLVFFSFILLVGGSLGNLID
                     RFRIGSVIDFLDFRIWPIFNIADTSLNIGIFLLIIHFLFQQKDDNKKSNMLEVEE"
BASE COUNT          895 a          478 c          455 g          798 t
ORIGIN      
        1 aaagagatag aaaatagtga acagtatcgt aaaaaaggtg agttgattat ggctaatttg
       61 tggaatataa aaagaggcat tgaccaggtc agcttagtgg attactctct tcccaaccat
      121 cccagagtag agattgcctt aaaccctgat ttatcaccac tgcaaaatgc ccatcaatta
      181 tttcaaagat ataaaaaatt aaatccaggt aaagaaagaa acagggagca gttattggaa
      241 aatcagaaat ctttaaagca attaaaggat atgttttcaa cattatatga aagtaaaaat
      301 tctctatctg aactgtctgc tctttatcag gaattaaccg ttcccaatta tattaaaaag
      361 aaaaaagtag gcaatggaaa acatttaaaa gggagagtgc cctctattca aaaacttatc
      421 tccagtgatg gatggacaat tctggtagga agaaataacc gacaaaatga atacctttta
      481 aggcatcttt ccaatggtaa tgatttctgg ttacataatc aaaccagacc tggggctcat
      541 gttttgataa aaaatcatca aggattaccg gttccccctc ccgatactct catctttgcc
      601 gcccgattaa ccggttatta tagtaaggtt aaaaacaatg aaacagcgca aatagtttat
      661 acttttagaa aatatgtaaa gaagcctaaa gactctatag ctggcaaggt aatttattct
      721 caggaaaaaa atatagctgt agttataaat tatgaagaaa taaagaaaga aataaataaa
      781 ttgatagtgg tttaaggggt agcaagcatc tttagctgtt ccagaaaatc atctggcaaa
      841 ggggaagtaa aggttaatga ttgttgcttt accggatgca cgatttctaa aaaatgtgaa
      901 tgcaaagcct gtctatctat tatcagggaa gggtcgtctc tcttaaatga accataaacc
      961 ttatcgccta ctacaggata tcctaaatag gatagatgga cccttacctg atgtgtacgc
     1021 cccgtatgaa gatctaatat taaaagagtg tgtgatgcaa aatatttgat caccttaaaa
     1081 taagtctttg cttcccgccc gccaatatta gtaaccgtca ttttattgcc atgttgaggt
     1141 gatcgaccaa tgggggcatc aatccaccct tcctcatctt tgaccagatt atgcaccaat
     1201 gcaatatatt tttttattac ttttctctct ttaatctgta tgctcaaaga tctgtgggca
     1261 agttctgttt tggcaatcat cattaaacct gaggtttctt tgtccagacg gtgcacaata
     1321 ccaggacggt ttatcccact gatgccagaa agctgcccct gacaatggaa aagcagggca
     1381 ttgaccagag taccactgtg tacctttccg gtgggatgga caatcatacc ggcagctttg
     1441 tcaatgacca tcatatgctc gtcctcataa taaatattaa gaggaatgtt ttctggctgt
     1501 aaaactaatt cacggttatc aggtatagct aaagaaatat tatcacctga ttttaaaata
     1561 tgactgggct ttatttccct gccgtttaca gtgatatgcc cctgatttat caattctttt
     1621 atcctgtttc ttgataaacc agctatgttc tccttagcca ggaaaatatc aactctaacc
     1681 ctttcttttt cctgaaattg gatattatga acaaattttt gggaattttt gctgttcatg
     1741 atactatcac caaaatgggg atatgcctaa tttgatagtt ttcctggtaa aagattcttc
     1801 actcttccac ttccagcata ttgcttttct tgttgtcatc cttttgctgg aacaaaaaat
     1861 gaataattag taaaaatatc cctatattaa gagatgtatc ggctatatta aatataggcc
     1921 atatcctgaa atctaaaaaa tcaattacac ttcctattct aaatcgatct attagattac
     1981 ccagagagcc tccaacgagt aagatgaagg agaaaaatac cagtttatta tttctggcaa
     2041 tctttaataa aagaataatt ccaattagaa taattaccaa tccggtaata atgaaagtct
     2101 tattgctgaa ctttaacaag ccaaaagaag aaaaaggatt ctgaatataa gtgatatgaa
     2161 aaatattatt aataatggga atagattccc ccaattccat agtactggaa acccagactt
     2221 tgctgacctg gtcggtgaga attaccagca ataccccgat aataaaattc atttaatctc
     2281 cttgtttttt gttaaaaata tagtggttta tggcaagtta taagttaatg agtaaaaaca
     2341 gttgagagac ttcttctccc tcattttctt accataaaag aaaaaatcca atcatctcct
     2401 gtcattgcac gcaggttgat tatctttttt ctgtccttgt gagcctactc atggcaggca
     2461 cggtaatctc ataatgtgca gcccgtatca aaatgtgatt gaaaatgggt ggcagttcac
     2521 caaaaagtga aaaatataag ctataatgga aagaataaag atgctaaagg accgtccctt
     2581 aacagctttc agtttccaga actgaaacac acctggcaca aatatt
//
LOCUS       DAOQWT010000204         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_43427_length_2606_cov_20.247746, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000204 DAOQWT010000000
VERSION     DAOQWT010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43427_length_2606_cov_20.247746"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2606
                     /locus_tag="PKJ95_04740"
     CDS             <1..>2606
                     /locus_tag="PKJ95_04740"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR65583.1"
                     /translation="SPLITVPLEYVVTSPIAYIPDDNFRMAINEALGQPSEYQPTIAD
                     LNGLTGTLFASDRNIISIEGAQYLINLQQLRLYDNQISGLSALAELTNLQVLYLATNQ
                     ISDLSPLTGLFNLQNLWLDNNQISDLSPLDVLTNLQHLSLVNNQISNISPLADLTNLR
                     VLHLGHNQISDLSPLAGLTNLQNLDLGDNQISDLSHLAGLTHLYYLDLWYNQISDLSP
                     LVDITNLQILHISYNQISDLSPLADLTNLRVLHLSYNQISDLSPLSGLTNLQELNLSY
                     NPIAYESMLLSHSWTLPWSTNEYNPLSPCYPDPSRNATNVLVNYPLSWQGNYDHSQDI
                     FYEVWLGISPDSLFSQGLETQMVNVTEHLYSFTPMLEPLTQYYWRIRAIAPTDTIWSG
                     MWSFTTGEEFYQPIISVTPTIIYDQLTTAETSQTEITIANYGTATLIWNSNIVLTRAE
                     QKTGTNAYLTKENNTERELNAKIENIDANSIRDKDMNREGTSPMPEINLSPSSGTIEP
                     DSSVICILNFIPGDSLGTYNYELQLSSNDPVSPLITVPLEYVVTSPIAYIPDVNFRMA
                     INEALGQPSEYQPTIADLNGLTGALYAWSWNITSIEGAQYLTNLQELWLYDNQISDLS
                     PLTGLSNLNYLYLNNNQISNISPLTALTNLQDLYLRYNQISDLSPLESLINLRELDLY
                     YNQISDISPLAGLTHLNNLNLYYNQISDLSPLAGLTNLHNLYLKNNQISDLSPLAELT
                     NLYHLELAENQISDISPLAGLTNLNYLFLDYNQISDLSPLAGMSNLQYLWLNYNQISD
                     LSPLAGLTHMNNLDLYYNQISDLSPLAGFTNLNYLNLDGNPISYESMLLSQSWSLPWS
                     TDEYNPLSPCYP"
BASE COUNT          776 a          531 c          456 g          843 t
ORIGIN      
        1 tttcacctct tatcacggtt cctttagaat atgtagttac cagtcctatt gcctatattc
       61 ctgatgacaa ttttagaatg gcaataaatg aggcattagg ccaaccctct gaatatcaac
      121 ctactattgc tgatctaaat ggattaaccg gaactttatt tgcttctgat cggaatataa
      181 ttagtataga aggtgctcaa tatctgatta atctgcaaca gttgcggctt tatgataacc
      241 agataagtgg cctcagtgct ttagcagagt tgacgaatct acaagttcta tatcttgcta
      301 ctaaccagat aagtgatctc agtcctttaa ctggtttgtt taatctgcaa aatctgtggc
      361 ttgataataa ccagataagt gacctcagtc ctttagatgt gctaactaat cttcaacatt
      421 tgagtcttgt taataaccag ataagtaaca tcagtccttt agcggatctg actaacctgc
      481 gagtgctaca tcttggtcat aaccagataa gtgatctcag tcctttagcg ggtctgacta
      541 atctccaaaa tctggatctt ggtgacaacc agataagtga cctcagtcat ttagcgggtc
      601 tgactcatct gtattatctg gatctttggt ataaccagat aagtgacctc agtcctttag
      661 tagatattac taatctgcaa atactgcata ttagttataa ccagataagt gacctcagtc
      721 ctttagcaga tctgactaac ctgcgagtgc tacatcttag ttataaccag ataagtgatc
      781 tcagtccttt atcggggcta actaatctac aagaacttaa tcttagctat aatcctattg
      841 cttacgaatc aatgctgctg tctcatagct ggactttacc ttggtcaacc aatgaatata
      901 atcccctttc gccttgttat cctgatccca gtagaaatgc taccaatgtt ttggtgaatt
      961 atcctttatc ttggcaggga aattatgatc atagccagga tattttctat gaggtctggt
     1021 tgggcattag tcctgattcc cttttctctc agggcttgga aactcaaatg gtaaatgtta
     1081 cagaacatct ttattccttc actccaatgc tggaaccctt aactcaatac tattggagaa
     1141 taagagctat tgccccaact gacacaatct ggagtggaat gtggagtttc actactggtg
     1201 aggaattcta tcagccaatt atttctgtta ctcctaccat tatttatgat caattaacta
     1261 ctgcagaaac cagtcaaaca gaaataacta ttgctaatta tggcactgct acccttatct
     1321 ggaatagcaa tattgtttta acccgggcag aacagaaaac aggaactaat gcttatctta
     1381 ctaaggaaaa taacacagaa agagaattga acgctaaaat tgaaaacata gatgccaata
     1441 gcataagaga taaagatatg aatagagagg gaacatcccc aatgccggaa atcaaccttt
     1501 ctccttcctc aggaactata gaacctgatt cttcagtaat ctgtatcctg aattttattc
     1561 ctggagattc ccttggcacc tataactatg aacttcagct ctcatccaat gatcctgttt
     1621 cacctctgat cacggttcct ttagaatatg tagttaccag tcctattgcc tatattcctg
     1681 atgtcaattt tagaatggca ataaatgagg cattaggtca accctctgaa tatcaaccta
     1741 ctattgctga tttgaatgga ttaactggag ctttatatgc ttggtcttgg aatataacaa
     1801 gtatagaagg tgctcaatat ctgactaatc tgcaagagct gtggctttat gataaccaga
     1861 taagtgacct cagtccttta acaggtctgt ctaatctgaa ttatctatat cttaataata
     1921 accagataag taacatcagt cctttaacgg ctcttactaa tctgcaagat ttgtatcttc
     1981 ggtataacca gataagtgac cttagtcctt tagagagtct gataaatctg cgagagctgg
     2041 atctttatta taaccagata agtgacataa gtcctttagc agggctgact catctgaata
     2101 atctgaatct ttattataac cagataagtg acctcagtcc tttagcgggg ctgactaatc
     2161 tgcataatct atatcttaaa aataaccaga ttagtgacct cagtccttta gcggagctga
     2221 ctaatctgta tcatctggaa cttgctgaaa accagataag tgacatcagt cctttagctg
     2281 gtctgactaa tctgaattat ctatttcttg attataacca gataagtgac ctcagtcctt
     2341 tagcggggat gtctaatctt caatatctgt ggcttaatta taaccagata agtgacctca
     2401 gtcctttagc agggctgact catatgaata atctggatct ttattataac cagataagtg
     2461 acctcagtcc tttagcgggt tttactaatc tgaattatct taatcttgat ggtaatccta
     2521 tttcttatga gtcaatgctg ctatctcaga gttggtcttt accttggtca accgatgaat
     2581 ataatcccct ttcgccttgt tatcct
//
LOCUS       DAOQWT010000205         2561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_44658_length_2561_cov_3.407422, whole genome shotgun sequence.
ACCESSION   DAOQWT010000205 DAOQWT010000000
VERSION     DAOQWT010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44658_length_2561_cov_3.407422"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PKJ95_04745"
     CDS             complement(<1..665)
                     /locus_tag="PKJ95_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HNR65584.1"
                     /translation="MEAEEKKNTYLSNLRLGEILIKQGVIKPEQLKKALDAQKTDSKK
                     KIGDILISQGIISQKQLNQALQHVFETDYIELGDVVLDPEIISLIPKKIAVRYKLIPL
                     NKENNTLTLAMANPLDVNAIDYIKEYTKLDVIPKFANENEILNALSSSYEAGYQFDDL
                     LDRVDVATVSDMSDELNLSALEAISQEAPIIQLVNMVIVDAIKERASDIHLEPNQRGL
                     LVR"
     gene            complement(695..>2561)
                     /locus_tag="PKJ95_04750"
     CDS             complement(695..>2561)
                     /locus_tag="PKJ95_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNR65585.1"
                     /translation="VVKVDEEGYLVDIKYKMEGFLPASEDSLLREGQDKVNHTLELDD
                     EIDVYVEQVDEKNGLIHLSRERARYLILWDQLINAYKSKKPLQGEVVEKNASGLIVDL
                     GVKAFVPLSQVEQSFITEADLEKYVGKKLPFYILKMDKIKNKVILSHRLALEKEREFL
                     RQKTLSSLKVGQIVDGKVVNITDFGAFVDIGGIEGLLHISEITWKEIKNPLKLIQKGD
                     ELKVKVLNFDKDEKKISLSIKQINPNPWDVVEEKYRVGDEVEGKVINIKDFGVFIELE
                     EGLNGLLHISDMSWAYIRTPQDVLTENDVIKVRVLDIDKENKKISLGLKQLMEDPWQN
                     IEKKYAIGSTVEGKITQVTSYGANVELEPGVSGIIHLSEIDWKYIEKPSSVLKKYAVL
                     PLQVLKIDSKDRLVFLSRKHTLPNPWQNIEGKYKVNDKVNGIVKRLKNFGAFVSLDEE
                     VEGFLPFSEITWEDIKHPNQVLKKDDQLELKIIEINPEKYQITLSRKRFLPNPWTVIK
                     KKYEVGTIVEGKVVNITDFGAFVNLEENIDGLVPLSEISNHKIEQVDSVLALGDTVQT
                     IVTKIDDRRKKISLSIRKAEKEVQKRLVKDYNKKEGSERILLKDVFGDILNYKEK"
BASE COUNT          690 a          552 c          393 g          926 t
ORIGIN      
        1 aaacgaacta aaagacctct ctgattgggt tctaagtgta tatcgctggc tctctcttta
       61 atggcatcca cgatgaccat attcaccagc tgaataatgg gggcttcctg gcttatggct
      121 tccagggcag aaagattcag ctcgtcactc atatcgctga ctgtagccac atctacccgg
      181 tccagtaaat catcaaactg gtatccggct tcataagaac tgctaagagc attgagtatt
      241 tcattttcat tggcaaattt aggtatgaca tccaatttgg tgtattcttt gatatagtct
      301 attgcgttaa catctagagg attagccata gccagagtca gggtattgtt ctccttattg
      361 agaggtatca gcttataacg tacggcaatt ttcttgggaa taagagaaat tatttccggg
      421 tcaaggacaa catctcccaa ttctatatag tcagtctcaa aaacatgctg caatgcctgg
      481 tttaactgtt tttgagaaat tattccctga ctgattagaa tatctcctat tttctttttg
      541 ctatcagtct tctgggcatc caaagccttt tttaactgct ctggctttat cactccctgc
      601 tttatgagaa tttctcccaa tcttaaatta gaaaggtagg tatttttctt ttcttctgct
      661 tccaacctac tgctccttaa taatattttt cactttactt ttctttataa ttcagaatat
      721 ctccaaatac atctttaaga aggattcttt cgcttccctc tttcttatta taatctttta
      781 ccagcctttt ttgaacctct ttctctgcct ttctaatact gagactaatt ttttttcttc
      841 tatcatcaat tttagttact atagtctgaa cagtgtctcc cagtgccaga acactatcta
      901 cctgttcaat cttatggttg gatatttcag aaagaggtac cagaccatct atattttctt
      961 ccagatttac aaaagcacca aaatcagtaa tatttactac tttcccttcc acaatagtgc
     1021 ccacttcata ttttttctta attacagtcc agggattggg aaggaaacgt ttacggctaa
     1081 gggttatctg atacttttcc gggtttatct ctattatttt taattccagc tggtcgtctt
     1141 ttttgagcac ctgattgggg tgtttgatgt cttcccaggt aatttcagaa aagggcagaa
     1201 agccctctac ttcttcatca agtgaaacaa aggcaccaaa attcttcaat cttttaacaa
     1261 taccatttac tttatcatta accttatatt ttccctcaat gttttgccag ggattgggca
     1321 gggtgtgttt tctgcttaag aaaactaaac ggtctttgct atctatctta agtacctgta
     1381 agggtaatac agcatatttt tttaagacgg aagatggttt ttcaatgtat ttccaatcaa
     1441 tttcagaaag atggataatt cctgatactc ccggctctaa ttccacattg gcaccatatg
     1501 aggtgacctg ggtaattttt ccttctacag tacttcctat tgcatatttt ttttcaatgt
     1561 tttgccaggg gtcttccata agctgcttta agccaagaga aatttttttg ttttctttat
     1621 caatatccaa aactctcacc ttaatcacat cgttttccgt caatacatcc tgtggggtcc
     1681 tgatgtaagc ccaggacata tctgagatgt gaagcaaacc attcaaacct tcttctaatt
     1741 caataaatac cccaaaatcc tttatattga tgacctttcc ctcaacttca tcgccaactc
     1801 tatatttttc ttccaccaca tcccaggggt tgggatttat ctgttttatg ctaagagata
     1861 tttttttctc atctttatca aaattaagaa cttttacttt taactcatca cccttttgga
     1921 taagcttgag aggatttttg atttccttcc aggtaatttc agaaatatga agaagtccct
     1981 ctatgccgcc tatatcgaca aatgctccaa aatcggtaat atttaccact ttcccatcaa
     2041 caatctgtcc cacttttaag gaggagaggg ttttctgtct taaaaactct ctttcttttt
     2101 ccaaagcaag gcgatgggaa agaattacct tattttttat tttatccatt tttagaatat
     2161 agaaaggcaa ttttttgcct acatattttt ctaaatccgc ctcggtaata aaactctgtt
     2221 ctacctgtga tagggggacg aatgccttga cacctaaatc gactatgagc ccactggcat
     2281 ttttctccac aacctcaccc tgtaatggtt ttttggattt ataggcatta atgagctggt
     2341 cccatagtat taaatagcga gctctttctc tggaaagatg aataagtcca tttttttcat
     2401 caacctgttc cacataaaca tctatttcat catccaattc cagagtatgg tttactttat
     2461 cctgtccttc tctcagtaat gagtcttcag aagcaggaag aaatccttcc atcttgtact
     2521 ttatatccac aagatatccc tcttcatcaa ctttgactac t
//
LOCUS       DAOQWT010000206         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_44835_length_2554_cov_2.881553, whole genome shotgun sequence.
ACCESSION   DAOQWT010000206 DAOQWT010000000
VERSION     DAOQWT010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44835_length_2554_cov_2.881553"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..108)
                     /locus_tag="PKJ95_04755"
                     /pseudo
     CDS             complement(1..108)
                     /locus_tag="PKJ95_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003220322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            815..1690
                     /locus_tag="PKJ95_04760"
     CDS             815..1690
                     /locus_tag="PKJ95_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNR65586.1"
                     /translation="MSNHMPKTREDLKKELVRLKKDNLKKSDGIPIISISQVKKTAQT
                     FNLSPKEVEIAALEERIIPTRYRRNFNTVSFAEQITFLKSKVTVAGCGGLGGNIIELL
                     ARLGIGSIIAIDGDVFQESNLNRQALCNEDSFGTGKAVTAISRIKHINSSIDAHSYSQ
                     FFDIHNLATFIGDTDVVIDALDNIPSRFLLEKVCKELKIPLIHGAINGLQGQASTIFP
                     EDKGLVAIYGPEDKYHKQFTDNQVSVLSVTPAMIASIQVAEVLKVLLKRGKPLQNKLL
                     LINLEENDFNIVDIL"
     gene            2236..>2554
                     /locus_tag="PKJ95_04765"
     CDS             2236..>2554
                     /locus_tag="PKJ95_04765"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR65587.1"
                     /translation="MSFKVERKKIIQLLKDYFSRNSSLYHIEIAFLYGSWSKGFPHKE
                     SDIDLALLFSSPSQNEDILFDSINNISYDLSLKIGKEVNIIDIKEDFPYPMLYYNAII
                     YGIP"
BASE COUNT          897 a          398 c          414 g          845 t
ORIGIN      
        1 atttaaggca aaagcagtag caatagcagc atctatggca ttaccacctt gttttaatat
       61 ccttacacca gcttccgctg ctagaggatg agcagcagct accatgccat gggcggcaat
      121 agcatcctgc ggggtatgct tgtctaaagc aagtgaagtt aatggaatca ttgaaagaat
      181 taacaaaaga gtgcagcaaa atgtaataat atttttaaaa ctttttttca ttttttctcc
      241 ttccttttat atctaagaat ttattaatgt attactattt tttcacctcc agtctctatt
      301 tatttatcaa tactttactt tgttcattat tcgggcatta caaataaaag caaatttttt
      361 tacttaacta tttagagtaa cttaattggt attgagcatt gaatcgtaca aaagtgaaat
      421 tgttgttggc tatcaaaata aatcgggaat ttaactcctc tttattttta gtagtgcagt
      481 tgtttttata ataccttccc tatgtggtac agagaataga aaatattggg tactgaaaat
      541 acttctatat tatatatttt aattaaatac tatcatattg atgcttctta agcaatgttt
      601 ttaaattatt ttgatgacta ataagaattg aatctaaaac aggctatggc agagatagga
      661 gcattgactt gattgttata ataataaata gatattaagg aagatgagaa aaagctaatc
      721 taccattaaa cttaagatgt aaaaaaaatg attttgttac cggttaataa ttctggttat
      781 gtaaataata tttcatataa taggctagta gctaatgagc aaccacatgc ccaaaacaag
      841 agaagatttg aaaaaggaat tggtaagact aaaaaaagat aatctgaaaa agtcagatgg
      901 tattcctata atatccattt cccaggtcaa aaaaacagcc caaactttta acttatctcc
      961 caaagaggta gagatagcgg ctttggaaga aagaataatc cccactcgat ataggcggaa
     1021 ttttaatacc gttagctttg cagaacagat tacttttctc aaatcaaaag taactgttgc
     1081 cggttgtggc ggattgggag gaaacattat agaattgtta gccagattgg gaataggaag
     1141 tatcatagca attgatggag atgtatttca agaaagtaat ctaaatagac aagcattatg
     1201 caatgaggat agtttcggaa caggaaaagc agtaacagcc atatcaagga taaagcacat
     1261 aaattccagc atagatgccc attcctattc ccaatttttt gatatccaca atcttgctac
     1321 tttcattggg gatactgatg tggtaatcga tgcccttgat aatattccct ctcgctttct
     1381 tttagaaaaa gtctgtaaag aattaaaaat cccccttatt catggcgcca tcaatggatt
     1441 acaggggcaa gcatctacca tcttccctga agataaggga ttggttgcaa tttatggtcc
     1501 ggaagataaa taccataagc agtttacaga taatcaggtt agtgtacttt cagtcactcc
     1561 ggcaatgata gcgtcaatac aggttgcaga ggtcttgaaa gttttactga agagagggaa
     1621 gcctttacaa aacaaattgc tcctaatcaa tttggaggaa aatgatttca atatagttga
     1681 cattctgtaa atcctttttc taagacacct tccatggata gtcacgataa tttaaataat
     1741 taattatatc aattatcaga ttttacacta tataacatct gtggttgata aatattatag
     1801 atataaatga atgaaattgc agaatagtcg ttggtcgttg gtatttagca tttagttaaa
     1861 atacaatgca aagaggtctg ataaggtcgt agattcgcaa tgacgaagaa ggttgtcatt
     1921 acgagtgacc gtagggagcg aagcaatctc atccactacc acccacatct tttcttctta
     1981 ttctttcatt tctgccaaca ctctttttat ttgtattttc tttgttttta ccaaaatcca
     2041 aaaactatta actaaaaact tccctcattt attcccgtat ttaacagaga accgtcccct
     2101 gtaaagacaa ttcaattcaa ctatagtata ttttatgtta taatttgtga aataagacca
     2161 attaacatac cttaaaaaat ccttagaaga gaataaaaaa aatgggtaat gaaataataa
     2221 agcagattca tagatatgtc ttttaaagta gaaagaaaaa agataattca attattaaaa
     2281 gattactttt caaggaattc atctttatac catatcgaga tagcttttct ttatggctcc
     2341 tggtcaaagg gatttccaca taaagaatct gatattgatt tagcccttct tttttcctca
     2401 ccttctcaaa atgaagatat cctatttgat tctattaata atattagtta tgatttaagt
     2461 ttaaaaattg gtaaagaagt aaatattatt gatattaaag aagattttcc ttatcctatg
     2521 ctttattaca atgctataat ctatggtata ccca
//
LOCUS       DAOQWT010000207         2553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_44854_length_2553_cov_3.815452, whole genome shotgun sequence.
ACCESSION   DAOQWT010000207 DAOQWT010000000
VERSION     DAOQWT010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44854_length_2553_cov_3.815452"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKJ95_04770"
     CDS             complement(<1..229)
                     /locus_tag="PKJ95_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65588.1"
                     /translation="MSDDFVLVASDGVTELNYIDSGTTPPGEDFLLKVGYKNTTADKR
                     ISIENVWYPDMVGHAFNNTGGKFPLLFASIIK"
     gene            complement(244..666)
                     /locus_tag="PKJ95_04775"
     CDS             complement(244..666)
                     /locus_tag="PKJ95_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65589.1"
                     /translation="MSSLSYLAYTDDTGKIFFPTEPIIPPFPEIASHTKIYPGTIPAN
                     LDGTLGTPGVTVTTAPYTTVGGKFNISGDHIDYVTMLAIFNLFLEGKNLFLYHATTNY
                     PRYFIGVLEPPKFFPKQGAQRGEEESFGFSMDFIISSE"
     gene            complement(666..>2553)
                     /locus_tag="PKJ95_04780"
     CDS             complement(666..>2553)
                     /locus_tag="PKJ95_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65590.1"
                     /translation="EQKQGIYDAQKAHEGEKKAIEEVNKEQFKNKGEFTEKFNALKQT
                     AEAEKKTATERIKNLKEFQSQWQLSPEQNEKITIEINKQQDKVDKYLNQQNKKRVQEN
                     KKDLQEQSNDWNLYYKSLTIALRDGEISHSQYQAKIKQYQTEHNITRETNEKLWLSME
                     NAYSQESLKIKQTEIKAKKKEESEYKKELREYAKEELAREKEEAAQKVELRKTILAEQ
                     EALEKASMSGKSPAEQYELEIEFMNKRISLQEALVASEAKQGELYKSRLDSGQDLNST
                     ELKTLEIYIKDKAALQQMNNEYSGLVDKAAQAQRQELENYKTYEQLLVQTGKITQAEA
                     DKAIGFFEKRVSDGWKNYITPIIKQATEQGYNSLNEMQKKALGSAKEFIQTNITATDN
                     LTTGMDNVTNSTNRAADAAAKIGSNLKSSADQADSLAGKMQTIGNIIAAKGGGTPSGL
                     GEFMTPEEAFANLPGSGKSPTGTSDRVTDRDKAIAESFNQAAANANTMDNPYLVTEAT
                     PYSGLGTIQPSSNEGYDNPTNDAGARKFGGNLIKNIAMPIITKSLKDMISNTLSGMVS
                     SVDNRTNQTSYNNSQSSNSQLTQITNYYNNTLNNQQLNQSSPIARKTNDLADMLNVRF
                     GRQM"
BASE COUNT          649 a          500 c          416 g          988 t
ORIGIN      
        1 gtttaattat ggaagcaaaa agaagaggga atttaccccc tgtattattg aaagcatgac
       61 cgaccatatc aggataccat acattttcaa tagaaattct tttgtctgct gttgtgtttt
      121 tatatccaac ttttaataaa aaatcctctc ccggtggagt agtacctgag tcaatataat
      181 ttaactctgt taccccgtca cttgctacaa gaacaaaatc gtcactcata aaatcaatct
      241 cctttattct gaagaaataa taaaatccat actaaaaccg aaactttcct cttctcctct
      301 ttgtgccccc tgtttaggaa agaatttggg aggttctaaa acacctataa aatatcgtgg
      361 ataattggtt gtagcgtgat agagaaataa attctttcct tcaaggaaca gattgaaaat
      421 agctaacatc gttacataat caatatgatc tccacttata ttgaattttc cccctactgt
      481 agtataaggg gctgttgtta cggtaactcc cggcgttcca agagtcccgt caagattagc
      541 cggtattgtt ccggggtaaa tctttgtatg tgaagctatt tcagggaatg gaggaattat
      601 cggttcagta ggaaagaata ttttccccgt atcatctgta taagctaaat atgaaagact
      661 actcattaca tctgcctccc aaaccttaca ttaagcatat cagctaaatc attagtcttc
      721 cttgcaatag gggaagattg atttaattgc tgattattta aagtattatt atagtaattt
      781 gttatttgcg ttaactgaga attggaactc tgagaattat tatatgaagt ttgatttgtt
      841 cgattatcca cagaagaaac cataccggaa agggtattag aaatcatatc ttttaatgat
      901 tttgtaataa taggcatggc aatgtttttt ataagattac cgccaaattt ccttgcacct
      961 gcgtcattag tgggattatc atatccctca ttggaagaag gttgtatagt tccaagtcca
     1021 gagtaaggag ttgcctctgt aaccaagtaa ggattatcca tagtattagc atttgccgcc
     1081 gcctgattaa aactctctgc tattgcttta tctctatctg taacacgatc actcgttccc
     1141 gtaggactct tgccagagcc aggaagattt gcaaatgctt cttcaggagt cataaattct
     1201 cctaaaccag agggagttcc accaccttta gcggcgatta tatttcctat ggtctgcatt
     1261 ttaccggcaa ggctatctgc ctgatcagca gaagatttta aattgcttcc tattttcgcc
     1321 gccgcatctg ccgctctgtt cgtactattg gttacattat ccattccggt tgtcagatta
     1381 tcagttgccg taatatttgt ttgtataaat tcctttgcac ttccaagagc tttcttttgc
     1441 atttcattaa gggaattata cccctgctct gtagcctgtt ttattatagg ggttatatag
     1501 tttttccatc catcacttac tcttttttca aaaaatccta tagctttatc tgcttctgcc
     1561 tgagtaattt ttccggtttg aaccaataat tgttcatagg ttttatagtt ctccaactct
     1621 tgcctttgtg cctgtgccgc tttatctaca agaccagaat attcattatt catctgttga
     1681 agtgcggctt tatcttttat gtaaatttcc agagttttta attcagtaga atttaagtcc
     1741 tgtcctgagt ctaatctgga tttatagagt tctccctgct ttgcctcgga ggcaacgaga
     1801 gcttcttgta gggatattct tttattcata aactcaattt ctagttcata ttgctcagca
     1861 ggacttttcc cactcataga cgctttttct aatgcttctt gctctgcaag aatggttttt
     1921 cttaattcaa ccttttgagc ggcttcttct ttttcccttg caagttcttc tttagcgtat
     1981 tctctgagtt ctttcttgta ttcagattct tcctttttct ttgcttttat ttcagtctgt
     2041 tttattttca acgattcttg gctataagca ttttccatag aaagccagag tttttcattt
     2101 gtttcccttg taatattatg ctcagtttga tattgtttaa tttttgcctg atattgacta
     2161 tgtgatattt ctccatctct aagtgcaata gtcaggcttt tataataaag attccaatca
     2221 tttgactgtt cttgaagatc cttcttattt tcctgaaccc tttttttatt ttgctgatta
     2281 aggtatttat ccactttatc ctgttgttta tttatttcta tggttatttt ttcattttgt
     2341 tcaggagata attgccattg agattgaaac tcttttaggt tctttatcct ttctgtggcg
     2401 gttttcttct ctgcttctgc cgtctgtttt aaagcattaa atttttcagt aaattctcct
     2461 ttatttttaa actgttcttt atttacctct tctattgctt tcttttcccc ttcgtgggct
     2521 ttttgagcat catatattcc ctgcttctgc tct
//
LOCUS       DAOQWT010000208         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_45552_length_2530_cov_2.644444, whole genome shotgun sequence.
ACCESSION   DAOQWT010000208 DAOQWT010000000
VERSION     DAOQWT010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45552_length_2530_cov_2.644444"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1084
                     /gene="garR"
                     /locus_tag="PKJ95_04785"
     CDS             194..1084
                     /gene="garR"
                     /locus_tag="PKJ95_04785"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HNR65591.1"
                     /translation="MPKPKIGVFGIGIMGFPMAKNLIKAGYPLIAYSLTEQSLSKILN
                     EGAERGESCTHVAKNCDIIITMLPNSPDVEQVILGEQGILKGACKGQILIDMSSIAPL
                     VSQSLARELEKKGIDMLDAPVSGGQEKAQSGTLAIMVGGEEEIFNYCKPVLEVMGKPI
                     LVGDIGAGGTAKLVNQAIVAVNIAIVAEALLLGKKAGVDPERIVSAIKEGLAGSQCLT
                     DKAPRMLSGNYDPGFRIHLHVKDLTNVLQTSQELHNAMPLTAQVMEMMQALMADGHTE
                     LDHAGLALFYEKLNSISLKK"
     gene            1114..1800
                     /locus_tag="PKJ95_04790"
     CDS             1114..1800
                     /locus_tag="PKJ95_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR65592.1"
                     /translation="MKLLIEERRKEIDNIFSYIFQPEEPVTWQAGQYALYRIPHDNPD
                     NRGEVRIFTVSSPPFKEKLMLTTHYLFEESSSFKKALFAKKVGDEVELVKIDGKFTIN
                     NDAQKLVFIAGGIGITPFHSILLELEKEKVGKDIILIYSNKDEEHIVFKDTLDNLARG
                     YNRLNIKYIFSPQRADENLIKETVPVLKERVFYISGPMRLVKAVEETLYKLEVDKENV
                     KKDYFPGLNE"
     gene            1882..>2530
                     /locus_tag="PKJ95_04795"
     CDS             1882..>2530
                     /locus_tag="PKJ95_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNR65593.1"
                     /translation="MVANFSREEREGIVVIGGANIDIKGKSKNVIQWDTSNPGTIRVS
                     HGGVGRNIAHYLGLLNIPVKYLSAVGDDSEGREILEKLRNARVQAEEVVLSKKEPTGK
                     YVAILDEKGDLQVGVSDTEIMKRVTPRYLIAKAGVIKKNRFMIIDTNLTPQAIYHIAD
                     LCYREKIPLIADPVSAVKSRKLLGILSRISYLTPDISELSALSGVIIKNAADRQKA"
BASE COUNT          838 a          415 c          551 g          726 t
ORIGIN      
        1 tgtataatac tataaaatag aatagaaaat aagaaatata ccttacttta atattctatc
       61 atgttataga aaaaaaatct tttctattta tttgcatttg cccttaggta aaatgatttt
      121 tcttgaaaaa tgattattaa ataaataatt cattatcata ggcttttaac ttttttaaga
      181 aaggagtact gatatgccta agccaaaaat cggtgttttt ggaattggaa ttatgggttt
      241 tccaatggct aaaaatttga ttaaggcagg atatcctctc attgcttact ctcttactga
      301 acaatcttta tcaaaaatac tgaatgaggg cgcagaaagg ggtgaatcct gcacacatgt
      361 ggcaaaaaac tgcgatatta tcatcactat gttacccaat tctccagatg tggaacaggt
      421 tattctgggt gaacagggta tcttaaaagg tgcctgtaaa gggcaaatcc ttatcgatat
      481 gagctccatt gctcccctgg tttctcaatc actggcaaga gaactggaga aaaaagggat
      541 agatatgctg gatgccccgg taagtggagg gcaggagaaa gcccaatcgg gaaccttagc
      601 cataatggta ggcggggaag aagagatttt caattactgt aaacctgtcc tggaggtaat
      661 gggtaaacca atactggtag gggatattgg agcaggtggt actgccaaat tagtaaatca
      721 ggccattgtg gcggtaaata ttgctattgt tgccgaagcg ctgcttttag gtaaaaaagc
      781 cggtgttgac ccggaaagaa ttgtttctgc cataaaagaa ggattagcgg gtagtcagtg
      841 tcttaccgat aaagcgcccc gtatgctcag cggtaattat gaccctggtt ttcggattca
      901 tttacatgtt aaagacctta ccaatgtctt acagaccagt caggaactgc acaatgccat
      961 gcctttaaca gcacaggtaa tggagatgat gcaggcctta atggcagatg gtcatacaga
     1021 gcttgaccat gccggactgg cactctttta tgagaagctc aatagtattt ctttgaaaaa
     1081 ataattaaaa tataagtaac caaggagtaa aaaatgaagc ttttaataga agaaagaaga
     1141 aaagaaattg ataatatctt cagttacatt tttcaacccg aggaacctgt aacctggcag
     1201 gcaggacagt atgcccttta caggattccc catgataatc ctgataatag aggagaggtg
     1261 cgaattttta cggtttcatc accaccattt aaagaaaagc ttatgctcac aacccattac
     1321 ttatttgaag agagtagttc ttttaaaaag gcactctttg ccaaaaaagt aggggatgaa
     1381 gtggagcttg ttaaaataga cgggaaattt accataaata atgatgcaca aaaattagtc
     1441 tttattgccg gtgggattgg aataacccct tttcattcca ttctcctgga attagaaaaa
     1501 gagaaggttg gcaaagatat aatacttatt tacagcaata aagatgaaga acatattgtt
     1561 tttaaagata ccctggacaa tttagccaga gggtataaca ggttaaatat taaatatatc
     1621 ttttcacccc agcgggctga tgaaaattta atcaaagaaa cagttcccgt tctcaaagag
     1681 cgtgtatttt atatttctgg tcctatgaga ttggttaaag cggtagagga gaccctctat
     1741 aagcttgagg tagataagga aaacgtaaaa aaggattatt ttcctggctt gaatgaatag
     1801 cagatatacc tcttttaaag cattatcaga tatctgttat agcgatactt tattattata
     1861 agagagtgaa ggaaacccgt tatggttgcc aatttttcca gagaagaaag agaaggtatt
     1921 gtggttattg gtggtgccaa tatcgatatc aaagggaaat caaagaatgt tattcaatgg
     1981 gatacttcca atccgggtac tatacgggtt agccatggag gtgtgggcag aaatattgcc
     2041 cattatttag gactattaaa cataccggta aaatatttga gtgcggttgg cgatgactct
     2101 gaggggagag aaatactgga aaagctgaga aatgcccgtg ttcaggccga ggaagtagtc
     2161 ctgtcgaaga aagaacctac tggaaaatat gttgccattt tagatgaaaa aggcgactta
     2221 caggttgggg taagtgacac cgaaattatg aaaagagtta cccctcgtta tctaattgcc
     2281 aaagccgggg ttataaagaa gaatcgtttt atgattatcg ataccaacct taccccccag
     2341 gctatttatc atattgctga tttgtgttac cgggaaaaaa ttcccttgat tgccgatccg
     2401 gtatctgcgg taaaaagcag aaaacttttg ggtattttat ccaggattag ttatttaact
     2461 ccagatatca gtgagctgag tgcactttcc ggagtcatta ttaaaaatgc tgctgatcga
     2521 cagaaagcca
//
LOCUS       DAOQWT010000209         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_45698_length_2524_cov_2.553665, whole genome shotgun sequence.
ACCESSION   DAOQWT010000209 DAOQWT010000000
VERSION     DAOQWT010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45698_length_2524_cov_2.553665"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PKJ95_04800"
     CDS             <1..1044
                     /locus_tag="PKJ95_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65594.1"
                     /translation="GNQYKIMDTIADEVFLFEDTRLTLTKELSTRYIASKTGICQPNV
                     ARVINRLEKKNFIRSIKSHVKGEGSLITIILDSTSISNKGTLDIKNISNNKKSDSTLL
                     SNQENLSRKKDLKKDDSNTSLNIEQTIKEKPQKPSLDSLPDMKNFLTSSKESNSNNPA
                     INLPCPPAPTSNVGFSSLGSILPDVTKIQSPAPSDKAIIEGKAILQEKGFTAPQIQAI
                     TQRITDSIRKHTPKKPDNYFLTAIKNEKIKPPVTGSSAPAFRQIPPAPGAMVQVQKNS
                     FTETVNRWEEEKKKDSPEDILKKLMDLRPDDLYRVILDVDNSPAGKFVSLPKIKASLY
                     IAEFKKRFPEVTV"
     gene            complement(1321..2148)
                     /locus_tag="PKJ95_04805"
     CDS             complement(1321..2148)
                     /locus_tag="PKJ95_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR65595.1"
                     /translation="MEEKQELFTIQDIIRLTRISQNTIYRYIRQGKIKICKMIKSGKQ
                     VITIEKQELERFLGYSIKGDTTSGKQDTTNNSIDTTSGKQDTINGSIYDKILTKSDIK
                     ELFQELIQEQNTIIMKPIEEQALYRLGKIEQENNFLKAKVETLLQELEQYKSLPGPAD
                     MEKWKKEKEDLLSEGEIHRQEQEEKHRKELEAVKGKYEDYLQKEKEDLLSRAETIQKE
                     QDSRYKVELEKQKKDIEEAHRQELEKIKKQAEEEKKQIAETWRKELEEARRPWWKFW"
     gene            complement(2232..2423)
                     /locus_tag="PKJ95_04810"
     CDS             complement(2232..2423)
                     /locus_tag="PKJ95_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65596.1"
                     /translation="MKDIDRVMVLSLSVGPLIRTGGPSPTRETQTGSHSFLSDNSQGD
                     GCHITFPGEQAGLSFPFVE"
BASE COUNT          745 a          534 c          430 g          815 t
ORIGIN      
        1 ggtaatcagt ataaaattat ggatactatt gcagatgaag tttttttatt tgaggataca
       61 aggttgactc tcacaaaaga actttctacc cggtatatag ccagtaagac tggtatatgt
      121 caacctaatg ttgcaagggt cataaataga ctggaaaaaa agaattttat tcggtcaata
      181 aaatctcatg taaagggtga aggtagttta attaccataa tattagatag tacttctata
      241 tctaataaag gcacattaga tattaaaaat atatctaata ataaaaaatc agatagtact
      301 ttgctatcaa atcaagaaaa tctatcaaga aaaaaagatc tcaaaaaaga cgactccaat
      361 acttctttaa atatagaaca gacaataaaa gaaaaaccac agaaaccttc tcttgatagt
      421 ttacctgata tgaaaaattt tcttaccagc tctaaagaaa gtaattcaaa caaccctgct
      481 attaatttac cttgccctcc tgccccgaca agtaatgttg gtttctcttc cctgggaagt
      541 attctgccgg atgtaacaaa gatacagtct ccagctcctt ccgataaggc cataatagaa
      601 ggcaaggcca ttcttcagga aaaaggtttc actgctccac agatacaggc aattactcag
      661 agaattactg acagtattag aaaacatacc ccgaaaaaac cggataacta tttcctgact
      721 gctataaaga atgaaaaaat aaagcctcct gttactggtt catctgctcc agctttccgg
      781 cagattcccc cggctccggg tgccatggta caggtgcaaa agaattcctt cactgagaca
      841 gtcaatagat gggaagagga aaagaagaag gatagccctg aagatatact taaaaaactt
      901 atggatttaa gacctgatga tctatataga gtaattttag atgtagataa ctcacctgcc
      961 ggtaaatttg tatcactgcc aaaaattaag gcatctctct atatagcaga atttaaaaag
     1021 agattccctg aagtgactgt ataaaattat caaaaataac cctttatgaa aatggctcta
     1081 aaatggatat tttgcccatc ccctcgaaaa ggccggtaaa gggttcatgt atatttcagg
     1141 ggaaacaggg taaatgtttc tttgaaaatt tcttcagtga gtgtttttgc ccattcccgc
     1201 aaaaagtaca atagaatgtt ttcggtctaa acagggggaa tgttacgcag gaatttaagc
     1261 cctgttaaaa atagccttct gattgcatgg taacgatcta aattaaattt aggtcgttac
     1321 ttaccagaac ttccaccagg gccggcgtgc ctcctccagt tctttcctcc aggtttctgc
     1381 aatctgtttc ttttcctctt ctgcctgttt ctttatcttc tccagctcct gccggtgggc
     1441 ctcctctata tccttcttct gtttctctaa ctctacctta taccggctgt cctgctcctt
     1501 ttgaatggtc tcagccctgg acagaagatc ttctttttct ttttgcaggt aatcttcata
     1561 ttttcccttt actgcttcca gttccttcct gtgtttttct tcctgctcct gccggtgaat
     1621 ctctccctca gataacagat cttctttttc cttcttccac ttctccatat ctgccgggcc
     1681 tggtaatgat ttatactgtt ccagctcctg aagtaaggtt tcaacttttg cctttaagaa
     1741 attattctcc tgttctatct tccccaatcg ataaagggcc tgttcttcta tgggcttcat
     1801 tataatggta ttctgttcct gtatcagctc ttgaaaaagt tctttaatgt ctgattttgt
     1861 aaggatttta tcatagatac taccattgat agtatcctgt ttaccactgg tagtatctat
     1921 actattattg gtagtatctt gtttaccact ggtagtatct ccctttatag aataacctaa
     1981 aaaccgctct aattcttgct tttctatggt aataacctgt ttaccacttt ttatcatttt
     2041 acagatctta attttaccct gccggatata acggtaaatg gtattctgtg aaatcctggt
     2101 taacctgata atgtcctgta tagtaaataa ctcttgtttc tcttccatgg tacagcctcc
     2161 acaccggaaa atataaaaca ggtgaattac tccgtcatta taacaagagt ctcttcacct
     2221 gttccataga tctattccac aaaaggaaag gaaagtcctg cctgttcccc tgggaaggta
     2281 atatggcatc cgtcgccctg gctattgtca gataaaaaag aatggcttcc tgtctgtgtt
     2341 tctcttgtgg gtgacggtcc acctgtccgg attaacggac ctactgacag tgatagaacc
     2401 attactctgt ctatgtcttt caaaatgacc agctcttacc ctgggaaggt atccctgaaa
     2461 aactgccatt taacgggctt ttaccgcttt cataagtatt gataagtatt attcctatgg
     2521 cctg
//
LOCUS       DAOQWT010000210         2522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_45750_length_2522_cov_3.235914, whole genome shotgun sequence.
ACCESSION   DAOQWT010000210 DAOQWT010000000
VERSION     DAOQWT010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45750_length_2522_cov_3.235914"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PKJ95_04815"
     CDS             <1..884
                     /locus_tag="PKJ95_04815"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNR65597.1"
                     /translation="FNYGRLKGTGYLSILPVDQGIEHSAGASFAPNPIYFDPENIVKL
                     ALEGGCNAIASTLGVLGIISRKYADRIPFIVKLNHNELLSYPNHYDQRMFATVEQAYD
                     MGALGVGATIYFGSEESRRQIEEVSLAFSLAHQMGMFTVLWCYLRNEAFKKEGIDYHT
                     SADLTGQANHLGATIEADLIKQKQPTNNGGYLALKFGRTSPLVYKKLTSEHPIDLTRY
                     QVVNCYMGRIGLINSGGPSGDNDLQQAVKTAVINKRAGGMGIISGRKTFQKPMKEGIE
                     IFHAIQDVYLCQEIEVA"
     gene            complement(887..>2522)
                     /locus_tag="PKJ95_04820"
     CDS             complement(887..>2522)
                     /locus_tag="PKJ95_04820"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HNR65598.1"
                     /translation="LEGVMGSDLIGFHTYDYVRNFLDSILRLRGYENRLGIVNYQNRI
                     VKVDSFPMGIDFERFYNSSEQSKTKQEIKKINKKVKNYKVIISVDRLDYTKGILERLK
                     AYSTFLEDNPEYWEKVIMILVAVPSRTAVTQYQRLKEQVDEMIGYINGQFGKIGWSPI
                     WYLYRSLPFHELTALYYISDIALVTPLKDGMNLIAKEYIAAKKDALGVLILSEMAGVS
                     NEMGEAIIVNPNNRTQISWALKYALNMEEQEQQKKVLTLQRRLKNYDVKKWADDFKEN
                     LIKVKSEQQNLAAKYLSPKNRLRIIKDYQQSSRRLFLIDYDGTLVPYHQKFKQSIPSQ
                     EVLDTIQLLSQDYKNKIVIVSGREKYIIEEWFKNVPVDLIAEHGAWLKKDSSNWRTIE
                     PLKKKWKEEIKPILELYSNRTPGSFVQEKEYSLVWHYVNTEATFGRRRALELMDSLTY
                     LTSNLNLEINHGEKAIEIKAAGINIGITSYQWISEREWDFIMAIGDDWSDEQIFSHLP
                     DWAYSIKVGSGISQSKYNIHSYIEVRTLLKELAKKS"
BASE COUNT          753 a          461 c          450 g          858 t
ORIGIN      
        1 tattcaatta tggtcgacta aaaggaactg gatatctttc aattttaccg gtagatcagg
       61 gtatagagca ctctgccggt gcttcttttg ctcccaatcc tatctatttt gatccggaaa
      121 atatagtaaa gttagcctta gagggtggtt gtaatgcgat tgcctcaacc ttaggtgttt
      181 taggtattat ttccagaaaa tatgctgata ggataccttt tattgtcaaa ttaaatcata
      241 atgaattact ttcttatcca aaccactatg accagagaat gtttgccact gtagagcagg
      301 cttatgatat gggagccctg ggagtgggcg ccactattta ttttggttct gaggagtcgc
      361 gacggcagat agaggaagta tctttagcct tcagtttagc ccatcagatg gggatgttta
      421 ctgtattgtg gtgctacctc aggaatgaag cctttaaaaa agaaggaatt gattatcata
      481 cttctgcgga tttaacaggt caggccaacc atttaggtgc tactatcgaa gcggatctga
      541 ttaaacagaa acaaccgacc aataatggtg gttatctggc gttaaaattt gggcgaacca
      601 gtcctttagt ctataaaaaa ttaacttctg aacatcccat tgatcttact cgctatcagg
      661 tggttaactg ctatatgggg agaatcggtt taattaattc aggcggacct tctggtgata
      721 atgatttaca gcaggcagta aaaacagctg ttattaataa aagagcaggt ggtatgggta
      781 ttatttcggg aagaaaaaca tttcaaaaac cgatgaaaga aggaatcgag atattccacg
      841 ccattcagga tgtatattta tgtcaggaga tagaagttgc ctgaaatcag gacttttttg
      901 ccaattcttt taaaagagta cgaacttcaa tgtaagagtg aatattgtat tttgattgag
      961 aaataccgga acctactttg atagaataag cccaatccgg taaatgagaa aatatttgtt
     1021 catcagacca atcatcaccg attgccataa tgaagtccca ttctctttct gatatccact
     1081 gataagaagt aatgcctatg ttaattcctg ctgcctttat ttcgatagct ttttccccat
     1141 ggtttatttc taaattgaga ttagaggtca gataggtaag tgaatccatc agttccaaag
     1201 ctcgcctgcg tccaaaagtt gcttctgtat tgacataatg ccagaccaat gaatattctt
     1261 tttcttgaac aaaagaacct ggcgttcgat tgctgtataa ttctaagata ggtttaatct
     1321 cttctttcca ttttttcttt aaaggttcta tcgttctcca attactggaa tcttttttta
     1381 accaggcgcc atgttcggca attaaatcca ccggaacatt tttaaaccat tcttctatta
     1441 tatatttttc tcttccgctt acaatgacta tcttattttt gtaatcctgg ctcagcagtt
     1501 ggatagtatc aagtacttcc tgggaaggaa tggattgttt aaatttttga tgatagggaa
     1561 caagtgtacc atcataatct attaagaata aacgtcggga actttgctga taatccttaa
     1621 ttattcttaa tctattttta ggagataaat atttagcagc aagattttgt tgctcacttt
     1681 taaccttaat caaattctct ttaaaatcat ctgcccattt ttttacatca taatttttta
     1741 aacgtctttg taaggtcaat acttttttct gttgctcttg ttcctccata tttaaggcat
     1801 attttaaagc ccaggatatc tgagttctat tattgggatt aacgatgatt gcttcaccca
     1861 tttcattgct aactccagcc atttcgctta agataagtac tcccagagca tcttttttgg
     1921 cagcgatata ttcttttgca attagattca tgccatcttt aagtggagtt actaaagcga
     1981 tatcacttat atagtaaagg gctgttagtt catggaaagg caaagaacgg tataaatacc
     2041 agataggtga ccagcctatt tttccaaact gtccgttaat ataacctatc atctcatcaa
     2101 cctgttcttt taaccgttga tattgggtaa cagccgttcg ggaaggaacc gcaactaaaa
     2161 tcattatcac tttttcccaa tactctggat tatcttctag gaaagtagaa tacgccttca
     2221 atctttctaa aatacctttg gtataatcca aacggtcaac agaaataatg accttataat
     2281 tttttacttt cttgtttatc ttcttaattt cttgttttgt cttagattgc tcggaagagt
     2341 tatagaatcg ctcaaaatca attcccatag ggaaagaatc tacttttact attcggtttt
     2401 gataattgac aatacccaat ctgttttcat atccacgtaa acggagaatc gaatcaagaa
     2461 agtttcgaac atagtcatag gtatgaaaac cgattagatc agaccccatc actccttcta
     2521 at
//
LOCUS       DAOQWT010000211         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_45790_length_2521_cov_2.201541, whole genome shotgun sequence.
ACCESSION   DAOQWT010000211 DAOQWT010000000
VERSION     DAOQWT010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45790_length_2521_cov_2.201541"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..574
                     /locus_tag="PKJ95_04825"
     CDS             215..574
                     /locus_tag="PKJ95_04825"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNR65599.1"
                     /translation="MGKSNNGKRRFFLLIIVIILFFLFNKNIFAEILNRNRPIAVMIG
                     NSPKERKVQRGIGEADIIYEIEVEYPFTRLMALYFEDNNITIGPVRSSRYYFSRISIE
                     WSSFFVHCGGQIRKNSG"
     gene            543..758
                     /locus_tag="PKJ95_04830"
     CDS             543..758
                     /locus_tag="PKJ95_04830"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HNR65600.1"
                     /translation="MGDKFGRIQVDLIGEGKGNFFHSGNYQPIKWIKKNKDDQTVFYD
                     SNCLPITLEKGLTWIHILSLDSGVWFK"
     gene            780..1178
                     /locus_tag="PKJ95_04835"
     CDS             780..1178
                     /locus_tag="PKJ95_04835"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNR65601.1"
                     /translation="MKDIFQKLLDEAKEARLKAYAPYSKFTVGSAVLTEDGTIFTGCN
                     IENVSLGLSICAERVAIFKAVADGYKNIKAIAIIGDTEEPCSPCGACRQVMVEFSPNM
                     DVIMSNLQNKIKIIKAIELLPDAFTGFNIN"
     gene            1309..2070
                     /gene="recO"
                     /locus_tag="PKJ95_04840"
     CDS             1309..2070
                     /gene="recO"
                     /locus_tag="PKJ95_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNR65602.1"
                     /translation="MNRLFRAEGIVLKSIKLNEADKIVTIFSDDHGKIKAIAKGVRKT
                     KSQFGSSMENLTINRLLLYRGKNMNTISQSEIVNSFFSQSKDLFRYGLAINCAEIVDK
                     LTVEEDPNKKLYNLFKNLLLLLEDEQNPVLLTESFKWKLFTLLGYKPELERCIHCHSI
                     FNKEGHYIFDITKGGISCFSCQKNAGYYQIKMSNYLLKLLQRIQDADLKKIHNKKIDE
                     PILNELGKIADIYLKYHFEIENQSKHFLNRLKLFK"
     gene            2240..>2521
                     /locus_tag="PKJ95_04845"
     CDS             2240..>2521
                     /locus_tag="PKJ95_04845"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017358011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNR65603.1"
                     /translation="MNIQDIIFNLEKYWSKKGCLILTPYDTEVGAGTMNPSTFLRVLG
                     PEPWKTAYVEPSRRPADGRYGDNPNRVQQHYQFQVIIKPSPENIQEIYLQ"
BASE COUNT          886 a          340 c          458 g          837 t
ORIGIN      
        1 ggagcagcag ccagtggctt tttaatggtt aaaacagaac tctatgaaag ggttctgtta
       61 ttcattgtgg ccataatgct tatatcacca gacctattaa ccgatataat tggtattttt
      121 ggtttgtctc ttgctttatt tttacaatat aaaaaatata aaaaattata aataaaagaa
      181 attttaattt ttgttggaaa aaataagttt ttaaatggga aaatcaaata atggtaaaag
      241 aagatttttt cttctaatta tagtaattat tttatttttt ctttttaata aaaatatctt
      301 tgccgaaatt cttaatagaa atcggccaat tgcagtaatg attggcaatt ctcccaagga
      361 acggaaagtc caaagaggga taggagaagc tgatattatc tatgaaattg aagttgaata
      421 tccttttacc agactaatgg cgctttattt tgaagataac aatattacca taggtccggt
      481 aagaagcagt cgttactatt tttctagaat atcaattgaa tggtcatctt tttttgttca
      541 ctgtggggga caaattcgga agaattcagg ttgaccttat cggggaaggg aagggtaatt
      601 tttttcattc aggtaattat cagcccataa aatggattaa aaaaaataaa gatgaccaga
      661 ctgtttttta tgacagtaat tgtttgccaa taactcttga aaaaggattg acatggatac
      721 atatattgtc attagatagt ggagtctggt ttaaataaga tataaagaag gctgaataaa
      781 tgaaagatat ctttcaaaaa ttgttagatg aagctaaaga ggcaagatta aaagcctatg
      841 caccttattc aaaatttacg gtaggatctg cggtattgac ggaagatggc actatattta
      901 ccggttgtaa tattgaaaat gtttcccttg gtttgtctat ttgtgctgaa cgggtagcca
      961 tctttaaagc cgtcgctgat ggatataaaa atattaaggc aattgccatt atcggggata
     1021 ccgaagaacc ttgttcaccc tgtggtgcat gccggcaggt aatggtggaa ttttctccaa
     1081 atatggatgt gataatgagc aaccttcaga acaaaattaa aataataaag gcaatagaat
     1141 tgcttcccga cgcctttaca ggttttaaca ttaattaact caaaaattta tagttacaat
     1201 agtttcacac tctctttcac ctaaaatcag ttaatttgta aaattttata ttcttatggt
     1261 gttttttgac tgattttttt ctctttaaat ataaacagga gttttttaat gaatcgctta
     1321 tttcgagcag agggaattgt tttgaaaagt ataaaactga atgaagcaga taagattgtc
     1381 actattttta gtgatgatca tggtaagatt aaagctatag ccaaaggagt gagaaagaca
     1441 aaaagccaat ttggtagtag catggaaaat cttaccataa acaggctgtt actctataga
     1501 gggaaaaata tgaatactat aagtcaatca gagattgtga attctttttt ttcacagagt
     1561 aaagatcttt ttcgatatgg attggctatt aattgtgcag aaattgttga taaattgaca
     1621 gttgaagaag accccaataa aaaattatat aatttattca aaaatctttt attactgctc
     1681 gaggatgaac aaaatccagt tcttttaact gaatcattta aatggaaact ctttaccctt
     1741 ttaggctata agcccgaatt agaaagatgt atacattgtc atagtatatt taataaagag
     1801 gggcattata tttttgatat aaccaaaggt ggaatcagct gttttagttg ccagaaaaat
     1861 gcgggttatt atcaaataaa aatgagcaac tatcttctta aactgttaca aaggatacag
     1921 gatgccgacc tgaaaaagat acacaataaa aaaattgatg aacctattct aaatgaactg
     1981 gggaaaattg ctgatattta tctaaaatac cattttgaaa tagaaaatca gagtaaacac
     2041 tttttaaata gactaaaatt gtttaagtag atagattatt tgcggcaata tcgctttaga
     2101 tatttttgac atcataaaat gattttgtta tatttattac atcataatct ttatccctct
     2161 ctttttttga gatttatcat aggtttatgg agagggtttt atttataata aggtaaagat
     2221 ggtttaggca tatacaaaaa tgaatattca ggatattata tttaatttag aaaaatattg
     2281 gagtaaaaaa ggctgtctaa tacttacccc ttatgatact gaggtgggcg cggggacaat
     2341 gaacccatct acatttttga gggtactggg cccagaaccc tggaaaacgg cctatgttga
     2401 gccgtctaga agacctgctg atggacgata tggcgacaat cccaacagag tacagcaaca
     2461 ctatcaattt caggttatta ttaagccttc ccctgagaat atccaggaaa tttatttaca
     2521 g
//
LOCUS       DAOQWT010000212         2514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_45972_length_2514_cov_3.479463, whole genome shotgun sequence.
ACCESSION   DAOQWT010000212 DAOQWT010000000
VERSION     DAOQWT010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45972_length_2514_cov_3.479463"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PKJ95_04850"
     CDS             <1..704
                     /locus_tag="PKJ95_04850"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNR65604.1"
                     /translation="SYHTEEAANKLLPIIGENTSLLTLQNGLGNTEILSKIYGKEKVI
                     AGTTSFGATILGPGKVLHAGTGPTAIGELDGKLTTRIKDIAEVLNQSGIKTEINQNVL
                     GLIWSKLVINVGINALGVLMRVKNGEIVKGEFSPKIQRALVEEAVQVAKKKGIKLTHE
                     DMLNEVISICKKTENNINSMLQDVIRKRKTEIDFINGAIVKEGEMVNIPTPFNQMITE
                     LIRAIEEKYEKQINI"
     gene            719..1582
                     /gene="panB"
                     /locus_tag="PKJ95_04855"
     CDS             719..1582
                     /gene="panB"
                     /locus_tag="PKJ95_04855"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNR65605.1"
                     /translation="MEQIKKKTMIPDFIFMKLEGKKFTMVTAYDFALATQVNKTDIEM
                     ILVGDSAAMVMLGLQSTVPINLDTMIILAKAVVRGAPNTFIVGDMPFLSYEVSKEKAI
                     ENAGRFMKEAEVDCIKLEGGKRMADTVAAIVRAGIPVMGHIGLTPQSSSQLGGFRVQG
                     KNLIAAEKLLEDALTLEEAGAFSIVLEGIPNPVAKVITERLKIPTIGIGAGPFCDAQV
                     LVIHDLLGLFERFVPKFVKKYAQIGQEIVKALSEFDREVKTGLFPDEEHSYRLKEEVE
                     KEILNYLKKIK"
     gene            1642..2178
                     /locus_tag="PKJ95_04860"
     CDS             1642..2178
                     /locus_tag="PKJ95_04860"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNR65606.1"
                     /translation="MEREKIPEKTIKSEQLYQGKIVKLRKDTIILPDGRKAIREIVEH
                     PGAVVILAEREEHKIVLVRQFRKAVEEFLLELPAGTIELSEKIIDCARRELEEETGYI
                     AKNWQEIYHFYSAPGFCNEKLTLYYASNLIKTRSNTDQDEFIEIVELNREEVQLLIKN
                     RNIIDAKSLVGILWWLHK"
     gene            2175..>2514
                     /locus_tag="PKJ95_04865"
     CDS             2175..>2514
                     /locus_tag="PKJ95_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00375 family protein"
                     /protein_id="HNR65607.1"
                     /translation="MIEKNLNKYFVDLHVHIGRNERALPVKVTASRNLTFANIARECQ
                     VKKGIDIVGIVDCASPRVIEDIEKMLNLGEMEELLDGGLRYHDVVTIILGAEIECKEK
                     KGGLSHQIAYF"
BASE COUNT          922 a          343 c          544 g          705 t
ORIGIN      
        1 aatcttatca tactgaagaa gctgccaata agcttttacc gataataggt gagaatacct
       61 ccctgctgac tcttcaaaat ggtttgggca acactgagat attaagtaaa atatatggaa
      121 aagaaaaagt catagccgga acaacttcgt ttggagctac tatactagga ccaggcaaag
      181 tgttacatgc tggtactggc ccaactgcca ttggtgaatt ggatggtaaa ttaactactc
      241 gtatcaaaga tattgcagaa gttctcaatc aatctgggat caaaactgaa attaatcaga
      301 atgtattggg gttaatctgg agtaaactcg tgatcaatgt tggaattaat gctttagggg
      361 tattgatgag agttaaaaat ggcgaaattg ttaaaggaga attttctcca aaaatacaaa
      421 gagctctggt tgaagaggca gtacaggtag ctaagaaaaa aggaattaag ttaactcatg
      481 aagatatgct caatgaagtt atctctatct gtaaaaagac agagaataat ataaattcca
      541 tgctacagga tgttataaga aaacgaaaaa ctgagataga ctttataaat ggagctattg
      601 ttaaagaagg agaaatggtt aatataccca ctccctttaa tcagatgata actgaattga
      661 taagggcaat agaagaaaaa tatgagaaac aaataaatat ttagaaggag aaaacaagat
      721 ggaacaaatt aaaaagaaaa caatgattcc agatttcatt ttcatgaaac tggaaggaaa
      781 aaaatttacc atggtcactg catatgattt tgctttagct actcaggtaa acaagactga
      841 tattgaaatg atactggtag gagattcagc agcaatggtt atgttaggac tgcagagtac
      901 tgttccaata aatcttgata ctatgataat tttagccaaa gctgtggtga ggggagcacc
      961 taatacattt attgtaggag atatgccttt cctttcttac gaagtgagca aagaaaaagc
     1021 aatagaaaat gccgggagat ttatgaaaga agccgaagta gattgcatca aattagaagg
     1081 aggaaaaaga atggcagata cagttgcagc catagtcagg gctggtatac cagtaatggg
     1141 tcacatcggt ctaactcctc agagtagctc tcaattgggt ggttttagag ttcaaggcaa
     1201 gaatcttata gctgctgaga aattacttga agacgcctta accctagaag aagccggtgc
     1261 tttcagtatt gtactggagg gtattcccaa tccagttgct aaagtcatta ctgaaagact
     1321 taaaatcccc actataggaa taggcgctgg acctttttgt gatgctcagg tgttagtaat
     1381 acatgatttg ttgggtttgt ttgaaagatt tgttcccaaa tttgttaaaa aatatgcaca
     1441 aattggccag gaaattgtta aggcactttc agaatttgat agagaggtaa aaaccggcct
     1501 tttcccggat gaagagcata gttatcgtct aaaagaagag gtggaaaaag aaatcctgaa
     1561 ctaccttaaa aaaataaaat aattttcaag aagtctgatt aaaggcttag attattatga
     1621 ataataaagg tgaagtttag tatggaaaga gaaaaaatac cggaaaaaac tatcaaatct
     1681 gaacaacttt atcagggaaa aatagttaaa ctacgaaagg ataccataat tctccctgat
     1741 gggagaaaag ctatccggga aattgtagaa catcctggtg cagtagttat tttggctgaa
     1801 agggaagaac ataaaattgt attggtaaga caatttcgta aagctgttga agagttctta
     1861 ttggaactac cagccgggac cattgagctc tctgaaaaaa taatagattg tgcccgaaga
     1921 gaattggaag aagaaacagg atatattgca aaaaactggc aagagattta tcatttttac
     1981 tctgcacccg gtttttgtaa tgaaaaatta accctttatt atgccagtaa tttaattaaa
     2041 actcgaagca atactgatca ggatgaattt atagaaattg tagaactaaa tcgagaagaa
     2101 gtccaattat taataaaaaa tagaaatatt atagatgcta aatcattagt ggggatcctg
     2161 tggtggctcc ataaatgata gaaaagaatt taaataaata ttttgtagat ttacatgtac
     2221 atattgggag aaatgaaagg gctttaccgg ttaaggtgac tgcgtccaga aatttaacct
     2281 ttgccaatat tgcccgggaa tgtcaggtta aaaaaggtat tgatattgtt ggaattgtgg
     2341 attgtgcttc ccctcgagtt attgaagata ttgaaaaaat gctcaatttg ggagagatgg
     2401 aagaattatt ggatggaggt ttgcgttatc atgatgtggt tactattata ttgggtgcag
     2461 agatagaatg taaagagaaa aagggaggtt tgtctcacca gattgcctat tttc
//
LOCUS       DAOQWT010000213         2514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_45975_length_2514_cov_2.851972, whole genome shotgun sequence.
ACCESSION   DAOQWT010000213 DAOQWT010000000
VERSION     DAOQWT010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45975_length_2514_cov_2.851972"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /gene="ispD"
                     /locus_tag="PKJ95_04870"
     CDS             <1..604
                     /gene="ispD"
                     /locus_tag="PKJ95_04870"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNR65608.1"
                     /translation="LLTYTLDKFEESEKIDEIILVVRPQDHKKIEQEMINKYYYSKVK
                     FIIGGGSTRQESVYNGLMAIKEDEGIVLIHDGARPLLKEEMIVELIGMIGNFVGVIPA
                     VPIVETVKKVILSNMMVERTVDRNKFWTVQTPQAFQLKQIRDCYKKAMKDNILFTDDS
                     SLFEYYGGKIAIVRGSEENIKITTKVDLLLAEALIKKCHF"
     gene            611..1498
                     /gene="ispE"
                     /locus_tag="PKJ95_04875"
     CDS             611..1498
                     /gene="ispE"
                     /locus_tag="PKJ95_04875"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNR65609.1"
                     /translation="MSKNAIKLKSYAKINLYLNIGKKFTDGYHSIESIMQTINLFDEI
                     YLEETDNFGIHITSNNLEIPVDKNSIIHRAAKILMENIDKGINIFIDKKIPIASGLGG
                     GSSNIATILIGISKLFGLKLNKHQLINIGSHFGMDIPFFMVRGTVFARGRGEIVLPLK
                     PISPSVPMILINPGIKVSTKWAYHLFDKLVKNNIKEDIDINYFLEKNMAIKLTELSQI
                     IYNSFDSVLSEHFSIIEQIKNKLKKLGSIAVTVTGSGSTVYGIFNREEKINQAYNKIK
                     DEYHFVYKTRTIQAKNIFL"
     gene            1564..2037
                     /gene="ispF"
                     /locus_tag="PKJ95_04880"
     CDS             1564..2037
                     /gene="ispF"
                     /locus_tag="PKJ95_04880"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNR65610.1"
                     /translation="MRIGFGYDIHPLKKGRPLFLGGVKVNSDRGLEGHSDADVVIHSI
                     MDALLGASGQGDIGVHFPNDEISWKGISSMVLLNRTCILLEKKKYKIKNLDITIVVEK
                     PKLLTYYPDMIMNIASALKISEDCINIKATTNEGIGFIGKGEGIASFCVTLLEES"
     gene            2076..2450
                     /locus_tag="PKJ95_04885"
     CDS             2076..2450
                     /locus_tag="PKJ95_04885"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNR65611.1"
                     /translation="MSNDEYKNSENDTNKEQDNNLKFYPNMLPFATLIIWFIGILNGK
                     AWEYLGLMMNPETKEVKQDLKKAKISIDTIEFLLNQIKDELEKEDKRQITDMLANLQM
                     NYVEKYEESKKNAQSDKMNETK"
BASE COUNT          941 a          297 c          462 g          814 t
ORIGIN      
        1 gcttctcact tatactttag ataaatttga agaatcagaa aaaattgatg agattattct
       61 ggtagtcagg cctcaggatc ataaaaaaat agaacaggaa atgattaata aatattatta
      121 tagcaaagta aagttcatta ttgggggagg ttctaccaga caggaatctg tctataatgg
      181 gttaatggct attaaggaag atgagggcat agttttaatt catgatggtg ccaggccttt
      241 attaaaggaa gagatgattg ttgagctcat aggtatgatt ggtaattttg ttggagtaat
      301 tccggctgtt cccatagttg agactgttaa gaaggtaatt ctatcaaata tgatggtaga
      361 aagaacagtt gaccgaaaca agttttggac tgttcagact ccccaggctt ttcaattaaa
      421 gcaaattaga gattgttaca aaaaggcgat gaaagataat attcttttta ctgatgattc
      481 atcacttttt gaatattatg gtggtaaaat tgccattgtt cgtggttctg aagagaatat
      541 taagattact acaaaggttg acctgctatt agcagaagct ttaataaaaa aatgtcattt
      601 ttaattttaa atgtcaaaaa atgctataaa attaaaaagt tatgccaaaa taaatttata
      661 tttaaatatt ggaaagaagt ttactgatgg ttaccatagt attgaaagta ttatgcaaac
      721 tataaatcta tttgatgaaa tctaccttga agagacagat aactttggga ttcatattac
      781 cagcaataac ctggaaatac cagttgataa aaatagtatt attcatagag cagctaaaat
      841 attaatggaa aatattgata aaggtattaa tattttcatc gataaaaaga tacctattgc
      901 ttctggtttg ggtggaggtt catcaaatat tgccaccatt ctgataggca ttagtaaact
      961 ttttggttta aagttgaaca aacatcaact tatcaatatt ggtagtcatt ttggtatgga
     1021 tattcctttt tttatggtta gaggtactgt ctttgctagg ggaagaggtg aaatagtttt
     1081 acctctcaag cctatttctc cttcagttcc tatgatactt attaatccag gaattaaagt
     1141 ttctaccaaa tgggcctatc atctttttga taaattggtt aaaaataata ttaaagagga
     1201 tattgatatt aactattttt tagagaagaa tatggcaatt aaactaactg aattatcgca
     1261 aattatctac aatagttttg attctgtcct ctctgaacat ttttcaatta ttgaacagat
     1321 taaaaataaa ttaaaaaaac ttggctctat tgctgtaacg gttaccggta gtggttcaac
     1381 cgtatatggt attttcaata gagaggagaa gattaatcaa gcttataata agattaaaga
     1441 cgaatatcat tttgtttata aaactcgaac aatacaggcg aaaaatattt ttttataaga
     1501 atgcttgtct gtcttgtctg caatgattta ttatcttttc aatagtatag aaaagagaag
     1561 attatgagaa taggatttgg ttacgatata caccctttaa aaaaaggaag acctttattc
     1621 ctgggaggag taaaagtgaa ttctgacaga ggattagagg gacattccga tgctgatgta
     1681 gttattcatt ccattatgga tgctctatta ggagcttccg gacaaggaga cataggagta
     1741 cattttccaa atgatgaaat cagttggaaa ggaatatcaa gtatggtttt actaaatcgt
     1801 acctgtatct tgttagagaa aaaaaagtat aaaataaaaa acctggatat cacaattgtt
     1861 gtggaaaaac caaaattgtt aacttattat cctgatatga ttatgaatat cgccagtgct
     1921 ttaaaaataa gtgaagattg cattaatata aaggctacta ccaatgaagg tatcggcttt
     1981 atcggaaagg gagaaggtat tgcttcattt tgtgtcactc tattagaaga gagttgaaat
     2041 tgttcaataa attgcaagaa taaggagtat taaagatgag taatgatgaa tataaaaatt
     2101 ctgagaatga tactaataaa gaacaggata ataacctaaa gttctatccc aatatgctcc
     2161 catttgccac tttaattatc tggtttattg gaattttgaa tggtaaagcc tgggaatatc
     2221 tgggtttaat gatgaatcct gaaacaaaag aagtaaagca agatttgaaa aaagctaaaa
     2281 tttcaattga cactatagag tttctgttaa atcagataaa agatgagctc gaaaaagaag
     2341 acaaaaggca aatcacagat atgttagcaa atttacaaat gaattatgtt gaaaaatatg
     2401 aagagagtaa aaaaaatgcc caatctgata aaatgaatga aacaaaatag gtttaaaaat
     2461 agttttatta agaataaatt ctttgggaga aaaagcaatg attggaaata atat
//
LOCUS       DAOQWT010000214         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_46032_length_2512_cov_2.657306, whole genome shotgun sequence.
ACCESSION   DAOQWT010000214 DAOQWT010000000
VERSION     DAOQWT010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46032_length_2512_cov_2.657306"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PKJ95_04890"
     CDS             complement(<1..437)
                     /locus_tag="PKJ95_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR65612.1"
                     /translation="MKVHEDWRFKLNFSICHKIAENINKVIVGKSKAIELMLATLLAE
                     GHLLLEDVPGVGKTLLARALAKSIGGTFHRIQFTPDLLPSDITGFNIYNQKEGRFVFQ
                     RGPVMSNIVLADEINRTIPRTQSSLLESMQERQVTVDGTTMDLP"
     gene            complement(587..1912)
                     /locus_tag="PKJ95_04895"
     CDS             complement(587..1912)
                     /locus_tag="PKJ95_04895"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNR65613.1"
                     /translation="MNDISSTNREGFLVEKSQRVLLDFLKEGIVPATGCTEPIAVAYA
                     VARARKESQGKELERIEVQVDSNLYKNGLMVTIPGTSEKGLLPAAAFGFLAGKTEKGF
                     RVIEGLTEKDIRDAQLLLAQNKIQVTLNQEYIGLHIEVILFTDLEKIRVITKDRHLNI
                     ISVEKIPKDEHFKPFIIKDTKKKDSDKKIQEYSLEDFLNFSNDISLKELSFLEEGITM
                     NMTIAREGLTIEHGVSNSLTELIKDGLISDNMVTRAQVLCAAASEARMAGSRFPVMSV
                     AGSGNHGITVFLTNYAVAEKESLPRESLLRSLALTSLITFFIKSYTGTLSAMCGCGVA
                     AGIGASAGVVYLLGGNKEQIMGALFNMVGSISGVICDGAKEGCAYKLALASGWAVQSA
                     LLSWKGAIIHNTDGIAAPGFKQLFQNLGYLCDPGMIATDKAILDIMLKK"
     gene            complement(1905..2291)
                     /locus_tag="PKJ95_04900"
     CDS             complement(1905..2291)
                     /locus_tag="PKJ95_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNR65614.1"
                     /translation="MEKEVLINPEAPKAIGPYSPALKIGNLIFASGQTPIDPSTGNLI
                     VGDIEIQTKTCLDNLKAVLKPYGIGFDNVVKVTIFLRDMNHFSQVNEIYSQYFTDKYP
                     SRSCIEVSRLPKDAQIEIEAIAYCNE"
BASE COUNT          750 a          532 c          444 g          786 t
ORIGIN      
        1 ggaaggtcca tagtggtgcc gtcaacggta acctgtctct cttgcatact ctccagcaga
       61 ctggattgtg ttctgggaat ggtcctgtta atctcatcag ccagaacaat attggacata
      121 accgggcccc tttgaaaaac aaacctgcct tctttctggt tatagatatt aaatccagtt
      181 atatctgagg gaagcaaatc gggagtaaat tgaatgcggt ggaaagtgcc accgatgctc
      241 tttgccagag ctctggctaa aagtgtctta ccaacccccg ggacatcttc taatagcagg
      301 tgcccttctg ccagcagggt ggccagcatt aattcaattg ccttactctt acccacaata
      361 actttattaa tattttcggc aatcttatga cagatggaaa aattcagctt aaatctccaa
      421 tcctcatgaa ctttcattca taaaaaagaa taaaaaatac tttagtaaat ttctcactaa
      481 gtttagatta tatcaattga gtgatgctaa aacaagctaa tattatatag aaggccctcc
      541 ttgtaggttt aaatgtaacc agaatgcaac aaaaattatt caaaaattat tttttcaaca
      601 ttatatccaa aatcgcttta tctgtagcaa tcattcccgg atcacacagg taacctaagt
      661 tttgaaacaa ttgtttaaac cccggtgcag caattccgtc agtattatga atgattgccc
      721 ctttccagga taataaggca gattgcactg cccagccgga ggcgagtgct aatttatagg
      781 cacatccttc ttttgcgcca tcacagataa caccggagat tgagccaacc atattaaaga
      841 gggcacccat tatctgttct ttatttccac ctaaaaggta gaccactccc gcacttgctc
      901 caataccggc tgccactcca caaccacaca tagccgacaa tgtgccggta taggatttga
      961 tgaaaaaggt tatcaaactg gtcagggcga gggatctcaa tagactctct ctggggaggc
     1021 tttctttttc tgcaactgca taattggtca gaaaaacggt gatgccatga ttaccactcc
     1081 ctgcaacact cattaccgga aatctgctac ctgccatcct ggcttcagag gcagcggcac
     1141 agagaacctg agcccgggta accatattgt cggaaatcaa tccatcttta atcaattcag
     1201 tcaaagaatt ggaaacacca tgttcgatag ttaaaccttc gcgtgcaatg gtcatattca
     1261 tagtgattcc ttcttcaaga aaggacagtt ctttcaagga aatatcatta gaaaaattta
     1321 aaaagtcttc taagctatat tcttgaattt tcttatcact atcctttttt ttggtgtctt
     1381 ttataataaa gggtttaaaa tgttcatctt tgggaatttt ttctacactt atgatattga
     1441 gatggcggtc ttttgtgata actctgatct tttccaaatc agtaaagagg attacttcaa
     1501 tatgtagacc aatatattcc tggttgagag taacttgaat cttgttttga gccagtaata
     1561 attgggcgtc tctaatatcc ttttctgtta atccctctat aacccggaac cccttttcag
     1621 tcttaccggc aagaaagcca aaagcagcgg caggaagcaa gcctttttcg gaggttccag
     1681 gaatggtgac cataagacca ttcttgtata aattagaatc tacctgtacc tctattcttt
     1741 caagctcttt gccttgagat tcttttctgg ctcttgctac tgcataggca acagcaatag
     1801 gctcggtaca gccagtagcc gggacgattc cttctttgag gaaatcaagc aatactcttt
     1861 gacttttttc cactaaaaat ccctctctat ttgttgaact tatatcattc attacaataa
     1921 gcgattgctt ctatttcaat ctgggcatct ttagggagcc tggatacctc gatacagctt
     1981 ctgctgggat atttatccgt aaaatattga ctgtaaattt cattaacctg tgagaaatgg
     2041 ttcatatccc ttaaaaatat ggttacttta acaacattat cgaaaccgat gccataaggt
     2101 tttaagaccg cttttaaatt atccaggcat gtttttgttt gtatctcaat atctccaaca
     2161 atgaggtttc ccgtagaggg gtcaataggc gtttgccctg aggcaaagat cagattccca
     2221 atcttcaagg caggagaata gggcccgatt gctttggggg cttccgggtt tattaagact
     2281 tctttttcca ttttaactat aacactcctt tattttcaat agttctttat cgtattctaa
     2341 caaattagta ataggttatc aaaaaaaatg gttgtcagca gtcatttaaa agataataaa
     2401 ataaggatca atagtttgtc tatagcaaaa aacataaagt tacaataatt tctctacaaa
     2461 cttatagagt caatcccatt ctttctgtaa aatcatattg ccaccaattc ct
//
LOCUS       DAOQWT010000215         2506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_46192_length_2506_cov_3.649939, whole genome shotgun sequence.
ACCESSION   DAOQWT010000215 DAOQWT010000000
VERSION     DAOQWT010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46192_length_2506_cov_3.649939"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PKJ95_04905"
     CDS             <1..675
                     /locus_tag="PKJ95_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNR65615.1"
                     /translation="YCIQETKSHLSQIPGELINKPGYYTYFTQAEKRGYSGVALWTKK
                     KPINISDKLGIPRFDSEGRYIRADFPEFILFNIYFPNGKASLERLQYKMDFYETFLKK
                     VVDLLNEGHKIIICGDVNTAHKEIDLARPKENSNISGFLPIEREWIDRLLQTGFLDTF
                     RHFNTEPGQYSWWDYKSRARERNVGWRIDYFLISENLKDNLKSAFIQTEVYGSDHCPV
                     GIDIDL"
     gene            688..1938
                     /locus_tag="PKJ95_04910"
     CDS             688..1938
                     /locus_tag="PKJ95_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HNR65616.1"
                     /translation="MIKFIHFADIHLGVENYGRIDAHTGLHSRLNDFIRTLTTAIDIA
                     LEEKIDFALFCGDAYKNNNPNPTHQREFARQIFRLSEAGIPTVLINGNHDNPLTFGRA
                     SSLDIFHTLRIANIYVVTEPVLLRLPTAKGNIQVIGIPWPTRNQYLEKEEYRESDLHS
                     INKKIQKRLMKRITELISQLDMQLPRFLAGHLTVAEAIFSGSESYASLGNDPSLPLQF
                     LRETSLDYIALGHIHRYQNLNDSNTPPIVYCGSMDRINFGEEQDEKGFCIASIDEQGK
                     VNYEFIPLPVRNMLTIEIKIEDCEDPTDEFVSLINEYNLKEAIVRINYEASEEQNRRI
                     DFKKVESALKEAFLVTGINRNIQKKSVLRRSTLSEEMDLFSALKEYIQLQNWQKWEKD
                     LNSFTKILEQESLQMDSQPGKDEK"
     gene            1935..>2506
                     /locus_tag="PKJ95_04915"
     CDS             1935..>2506
                     /locus_tag="PKJ95_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR65617.1"
                     /translation="MIPVTLHLHNFLSYGRDVPPLDFTQFNIASLSGGNGQGKSALLD
                     ALTWALWGEGRKAQQEKKADRGLLKIGENEMWVDLVIELEGERYRVIRKFSLVKKRSY
                     SELELMVFDSKKDDFISLSAPSIKQTQQRINSILRMDYNTFINSAFILQGRVDEFTRK
                     SALERKQILSEVLGLSHYERLSVLAHRKAK"
BASE COUNT          816 a          454 c          517 g          719 t
ORIGIN      
        1 tactgtattc aggaaacaaa gtcacattta tcacagatac cgggagagct tattaataaa
       61 ccaggttatt acacttattt tactcaggca gaaaaaaggg gctatagtgg agtcgccctc
      121 tggactaaga aaaaacccat aaatatctct gataaattag ggatacccag atttgatagt
      181 gaaggacgtt acatcagggc agatttcccg gaattcatat tatttaatat ctattttccc
      241 aatggcaaag catccctgga aagattacaa tacaaaatgg atttttatga aactttttta
      301 aagaaagttg ttgatttgct caatgagggg cacaaaatta ttatttgtgg tgatgtcaat
      361 actgcccata aggagattga tttagccagg cctaaggaaa attctaacat atcaggtttt
      421 cttcctatag aaagagaatg gattgaccgc ctccttcaga ccggattctt ggatactttt
      481 cgccatttta acacagaacc gggtcaatac agctggtggg attacaagtc ccgggctcgt
      541 gaacgcaatg tagggtggag aattgattat tttttaatca gtgagaattt aaaggataat
      601 ctaaaatctg cctttatcca gacagaggtt tacggttctg accactgtcc cgttggcatt
      661 gatatagatt tatagtagga acaaaggatg attaaattta tccattttgc cgatattcat
      721 cttggagttg agaattacgg gcgtatcgat gctcataccg ggttacactc ccgcttaaat
      781 gattttatca ggacactcac tactgctatt gatatagcct tggaagagaa gattgacttt
      841 gctctctttt gcggagatgc ctataaaaat aataatccta acccaaccca tcaacgagag
      901 tttgccagac agatttttcg gttaagtgag gctggaatac caactgtttt aattaacggc
      961 aatcatgata atcccttaac ttttggcaga gccagttccc ttgatatttt ccataccttg
     1021 cgtattgcca atatttatgt ggttactgag ccggttctcc tacgcttacc cactgcaaaa
     1081 ggtaatattc aggtaatagg aataccctgg ccaacccgaa atcaatacct tgagaaagag
     1141 gaataccggg aaagtgattt acattcaatc aataaaaaga tacagaaacg tctcatgaaa
     1201 agaataactg aactgattag tcagcttgat atgcagctcc ccaggttctt agcaggccac
     1261 ctgacagtag ccgaagccat cttttctggt tcagaaagct atgcttccct tggcaatgac
     1321 ccctctttac cactacagtt tcttagagag acctccttag attatattgc cctgggccac
     1381 attcatcgct accagaattt aaatgattct aataccccgc ctattgttta ctgtggtagc
     1441 atggaccgca ttaatttcgg agaagagcag gatgaaaagg gtttctgtat agccagtatt
     1501 gatgaacagg gaaaggtgaa ttatgagttt attcccctgc cggtacgcaa tatgctgact
     1561 attgaaataa aaattgagga ttgtgaagat ccaactgatg agtttgtatc cctgattaac
     1621 gaatataacc ttaaagaggc aatagtgcgt ataaactatg aagcaagtga agaacaaaac
     1681 agaagaattg actttaaaaa agttgaaagt gccttaaaag aagcttttct ggttacaggt
     1741 attaacagaa atattcaaaa aaaatcagtc ttgagaagga gtacactttc tgaagagatg
     1801 gatttattct ctgccttaaa agaatatatc caattacaaa actggcaaaa atgggaaaaa
     1861 gatttaaatt cttttactaa aatattagaa caggaatccc ttcaaatgga ctcgcagccg
     1921 ggaaaggatg aaaaatgatt ccggttaccc tgcacttaca taattttctg agttacggca
     1981 gagatgtgcc tccactcgat tttactcaat ttaatatagc cagcctatcc ggtggaaatg
     2041 gccaggggaa atcagcctta ctggatgccc tgacctgggc cttatggggt gaggggagaa
     2101 aagcccagca agagaaaaaa gcggatagag gattgttgaa aataggcgaa aacgaaatgt
     2161 gggttgatct ggttatcgaa ctggaagggg aaaggtatag agttatcaga aaattttctc
     2221 tggttaaaaa gcgcagttat agtgaattag aattgatggt ctttgattct aaaaaagatg
     2281 attttatttc tttatcagcc ccatccatca aacaaactca acagagaata aattcaatat
     2341 tgagaatgga ttataatact tttattaatt ctgcttttat cttacagggc agggtagatg
     2401 aatttacccg aaagagtgca ctggagcgta aacagatcct ttctgaggta ttgggacttt
     2461 ctcattatga acggctttca gtgctggctc accgaaaagc aaaaag
//
LOCUS       DAOQWT010000216         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_46216_length_2505_cov_5.715918, whole genome shotgun sequence.
ACCESSION   DAOQWT010000216 DAOQWT010000000
VERSION     DAOQWT010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46216_length_2505_cov_5.715918"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..559
                     /locus_tag="PKJ95_04920"
     CDS             224..559
                     /locus_tag="PKJ95_04920"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HNR65618.1"
                     /translation="MTHIERRYETYKQDQHNLTVYRVAGGYRVNNFRTNKDYYISKPD
                     GVVTCTCRDYKNRRKPCKHIIAVKEAHEEITKQTEKMTELNSFADSLMKKAQEIKRKQ
                     DIQKVIARW"
     gene            617..1459
                     /locus_tag="PKJ95_04925"
     CDS             617..1459
                     /locus_tag="PKJ95_04925"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HNR65619.1"
                     /translation="MSKQITEIITEKIINALEHGEVPWKKTWINAPKNGISKKEYRGI
                     NRLLLSMTDFSSPYFLTFKQASALGGYVKKGSHGIPVVYWHLCKVKKNEEEETTEISE
                     NTIPFMRYYIVFNLEQVEGIDRGKFTEHKRDFKPLETCEQIVKGYKASPIILHQGFQP
                     CYIPSTDTVEIPICENFTSEENYYSTLFHELTHSTGHKKRLNRKEVTFTQRFGSCNYA
                     EEELVAEIGASFLCSYAGIENKTIDNSTAYIQNWLKVLNDDRKMVINAASRAEKSMKH
                     IIGN"
     gene            1507..2130
                     /locus_tag="PKJ95_04930"
     CDS             1507..2130
                     /locus_tag="PKJ95_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65620.1"
                     /translation="MCNNNGKFNPQNHLMKVKGKDYLEVKYRVHWFRQEKPDWDIQTR
                     IIQVDIEKGLAIVQADIFDEEGRHKSSGIQMEEKKTFPDYLAKAETASIGRALACLGY
                     GTLQCFELEEGIEKGRIVDAPVSLNGDKGNGNTVNQTEKEQGQSPQENNGNNGKVKAT
                     MMQIRYLKDLCKNLDIKPAVDLAGLSSEKASRYISDLEKMRKLAVKV"
     gene            2193..2321
                     /locus_tag="PKJ95_04935"
     CDS             2193..2321
                     /locus_tag="PKJ95_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65621.1"
                     /translation="MKFSALGSGAAHPAARQIRTSAPEHLPRLKPPLTSLFFCCFV"
BASE COUNT          908 a          442 c          578 g          577 t
ORIGIN      
        1 agaaaggaag tgatctaaat gtggcttgga attgaatatt acataccgga taatgacggt
       61 gaagacgaag aagaaaagcc ggttgaaaca aaggaaatca attttgaaga cattccattt
      121 taggttaaaa gttgaagggg agcagtgcag ctaacactgt tctcccctgg tcaaaactaa
      181 tttaaaacca atcaaataat aacatagaaa ggggaaataa acaatgacac acatagaaag
      241 aagatatgaa acctataaac aggatcagca taacctgaca gtgtacaggg tagccggcgg
      301 ttacagggta aataacttca gaaccaataa ggattattac ataagtaaac ctgacggagt
      361 tgttacctgc acctgtagag actataagaa cagacgtaaa ccctgcaagc atataattgc
      421 agtaaaagaa gcacatgaag agattacaaa acagacagag aaaatgacag agttaaacag
      481 ctttgcagat agccttatga aaaaggcaca ggagataaag agaaaacagg acattcaaaa
      541 ggtaatagcc agatggtaaa ggcaatgggg agaggtaaag cctctccctg aatttttcag
      601 agaaagggga ttaaccatga gtaaacagat aacggaaatc ataacggaaa aaataataaa
      661 tgcccttgaa catggcgagg taccatggaa aaaaacctgg ataaatgctc cgaaaaacgg
      721 catttcaaag aaagaataca gaggaattaa cagactgctg ctttcaatga ctgattttag
      781 ttcgccttat tttctgacct ttaaacaggc cagtgctctg ggaggatatg ttaaaaaagg
      841 ctctcatgga attccggttg tttactggca tttatgcaaa gtaaagaaaa atgaggaaga
      901 agaaacgact gagatatcag aaaataccat accttttatg agatactaca tagtttttaa
      961 ccttgaacag gttgaaggaa tagacagggg caaatttact gaacataaga gagattttaa
     1021 acctctggaa acctgtgaac agatagtaaa aggatataaa gccagtccga ttatactgca
     1081 tcagggattt caaccatgtt acattccttc gactgatacg gtggaaattc cgatatgtga
     1141 aaattttact tcagaagaaa attactattc tactttattt catgaattaa cccatagcac
     1201 aggacataag aaaaggctga acaggaaaga ggttactttt actcagagat tcggaagttg
     1261 caattatgca gaagaagagc tggttgcaga aataggagct tcgtttctgt gcagctatgc
     1321 aggcatagaa aacaaaacta ttgataattc gacagcttat atacaaaact ggcttaaggt
     1381 tttaaatgac gatagaaaga tggtaataaa cgccgcaagc agagcagaga aatctatgaa
     1441 gcatattatt ggaaattaat aataacggcg ggggctccgg cccctgctga taaggaggaa
     1501 tataaaatgt gtaataataa tgggaaattt aatccgcaaa atcaccttat gaaggtaaag
     1561 gggaaggatt atcttgaagt aaaataccgg gttcactggt tcagacagga aaaacctgat
     1621 tgggatatac agacaagaat tatacaggta gatattgaaa aaggtctggc catagtacag
     1681 gctgatatat ttgatgaaga agggaggcat aaaagctcag gaatccagat ggaggaaaag
     1741 aaaacttttc cagattacct tgcaaaggcg gagacggcga gcataggcag agcactggcc
     1801 tgtttaggtt atggaacttt acaatgtttt gaacttgaag agggaattga aaaagggcgg
     1861 atcgttgacg ctcctgtaag tttgaacggt gataaaggca atggcaatac tgtaaaccag
     1921 acggaaaaag aacagggaca gagcccgcag gagaataacg gcaataatgg aaaagttaaa
     1981 gcaactatga tgcagataag atatctgaaa gacctgtgta aaaacctgga tataaaaccg
     2041 gctgttgacc ttgcagggct ttcaagtgaa aaagcaagtc gttatatttc agacctggag
     2101 aaaatgagaa aattagcagt taaagtgtaa gtttaaagcg gagccggtgg agtttgccgg
     2161 ctccgggtag tttttccgcc tggagctggt gaatgaaatt ttccgccctg ggctccggtg
     2221 cagcccatcc agcagcccgg cagatccgaa cctccgcccc tgagcacttg ccccgattaa
     2281 aaccaccctt aaccagcctc tttttctgct gcttcgttta gtcgggcagt gatgataaaa
     2341 acagatgtca atttttccgg catttttccc ggacatccgc cgggccccgc cggtgcagaa
     2401 atagaacagg atgaagacag agccgggaga cctccggcac atccagaccc ccgccctgag
     2461 caattacaaa aaattaaacc actcttaacc agcctctttt ttttg
//
LOCUS       DAOQWT010000217         2504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_46258_length_2504_cov_3.146999, whole genome shotgun sequence.
ACCESSION   DAOQWT010000217 DAOQWT010000000
VERSION     DAOQWT010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46258_length_2504_cov_3.146999"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1301)
                     /locus_tag="PKJ95_04940"
     CDS             complement(66..1301)
                     /locus_tag="PKJ95_04940"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HNR65622.1"
                     /translation="MIESIKLISVIMLIIIMLRLRWDLGLTMFLSSILLNFLFHISFK
                     GLLYNIYFTVTDPTTLQLIGIVVLVYILSGILKRTKSMEGIVYSLQQIVSDYRIILFL
                     IASFLGLIPMVSGAMFSAPLLKEIGEENQMSPEEIMLSNYWFRHVWELFLPLIPGVVL
                     YISIIGVSVRDIVIAQFPLTIFALSIGFFWMYLTLRKINKIKITHANLNQNLFTFLKH
                     GWSILLVLLLVISIKMNLLGSLIITIILLLIIKRVPFTTIKEIIIHDISIKILFMIIG
                     IMLFKQVLNNTNSLEPISHFLSEKGVNIWIILFFIPFILGLLTGITAGFVGISFPIIL
                     PLMLNNGSLNLSMAMFAYLAGFAGMMVSPMHLCLALTVEYLKVDITKFYKKLSLNLLA
                     LIIMSFAYIIFFNITISLS"
     gene            complement(1477..1977)
                     /locus_tag="PKJ95_04945"
     CDS             complement(1477..1977)
                     /locus_tag="PKJ95_04945"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNR65623.1"
                     /translation="MAITADEKNENKSEVEKKDLELGKISVSKDVVAIIVAMEAIKVK
                     GVVGLTAGRKGMSAPILEKNNLTKGIEVSMNQNQKEVALTVSLVADYTVGIYQTALET
                     QKNVKKAVETMAGLKVTKVDINVLDVKFKEDLEKEKKKEEITKEPPVVAKEQPEAPKS
                     VEEIKK"
     gene            complement(2016..>2504)
                     /gene="amaP"
                     /locus_tag="PKJ95_04950"
     CDS             complement(2016..>2504)
                     /gene="amaP"
                     /locus_tag="PKJ95_04950"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HNR65624.1"
                     /translation="FSLKCFLISFGIIKIEYLLKVFNEWLYLIYSNSIYQAFFGLIGL
                     FIFLIALYLIWLNQKIGQLLPHVKVITDSGEIRISTYSLEQIVLNILNKVEGVRKIKP
                     KIEIQKSEDIKTILELIIDKNCNIPHTASLIQQKLKEELPKISGVEAKEVKINVDKIE
                     YD"
BASE COUNT          857 a          449 c          337 g          861 t
ORIGIN      
        1 aatatgttaa tatttaagaa cagatttcag gctcatcttg cattggagaa gactacaact
       61 attcttcaag ataggcttat ggtaatatta aaaaatatga tataggcaaa gctcataatt
      121 attaaagcaa gcaaatttaa ggacaacttt ttatagaatt tagtaatgtc aaccttcaga
      181 tattctacag tcaaagccag acagagatgc atgggagaaa ccatcatacc tgcaaatcct
      241 gccaaatagg caaacattgc catggataaa tttaaagaac cattattgag catcaaaggt
      301 agtataatag gaaaacttat tccaacaaaa ccggcagtta ttcctgttag taatcccaat
      361 ataaaaggaa taaaaaacag aataatccat atatttaccc ctttttcaga taggaaatgc
      421 gaaattggtt ccagagagtt tgtgttattt agtacctgtt tgaataacat aattcctata
      481 atcataaata atatttttat ggaaatgtca tgaatgataa tttcttttat agttgtgaaa
      541 ggtactcttt ttataatcaa gagtaaaata atagtaataa ttaatgagcc aagcagattc
      601 attttaatgg atattaccaa taataaaacc aacaatatgg accatccatg ctttaaaaag
      661 gtaaaaagat tttgattcaa attagcgtgg gttattttga ttttatttat ttttctcaga
      721 gttagataca tccaaaagaa tccaatagaa agagcaaaga tggttaaggg aaattgcgca
      781 atgacaatat cccgaacact aaccccaata attgaaatat aaagtactac tccagggatc
      841 aatggtaaaa ataattccca tacatgacga aaccagtaat ttgagagcat aatctcctca
      901 ggactcatct ggttctcttc tccaatttct ttcagcaaag gtgctgaaaa catggcaccg
      961 gataccatcg gtattaaacc taaaaaagag gcaatcagaa aaagaatgat acgataatcg
     1021 ctcactattt gttgcaaaga gtatacaata ccttccatac tttttgttct tttcaaaatt
     1081 ccactcagta tgtaaaccag aactacaatc ccgattagct gtaatgtggt tggatcagta
     1141 acagtaaaat aaatgttgta caataggcct ttaaaactaa tatgaaataa aaaattaagt
     1201 aaaattgagg atagaaacat agtcaatccc agatcccacc tcaaccttag cattataatg
     1261 attagcataa taactgaaat taatttaata ctttcgatca aaaaattcta cctccattta
     1321 taagctcagt tgtaaaataa aaatatcctt ttttctcatc cttatctctg tcaacaccgt
     1381 gcaaatcaat tattttctcc cttgattaaa aatattccct taaaatcata ataattctaa
     1441 gggaatatct taaaatttat ggttttcgtt aaagtattac tttttaattt cttctacact
     1501 tttgggagct tcaggttgct cttttgccac tacgggaggc tcttttgtaa tttcctcctt
     1561 tttcttctcc ttttctaaat cttctttgaa tttaacatcc agcacattta tatccacctt
     1621 agttactttt aaaccagcca tagtttcaac agccttttta acattctttt gggtttccag
     1681 cgcagtctga taaatgccaa cggtgtaatc tgccaccagc gagacggtta aggcaacttc
     1741 tttctgattc tgattcattg atacctcaat tcccttggtt aaattatttt tttccagaat
     1801 cggtgcagac atacccttcc tgcctgctgt taaaccgact acacccttta cttttattgc
     1861 ttccattgcc acaataatag caacaacatc tttggaaaca gatattttcc ctaattctaa
     1921 atctttcttt tctacttctg atttattttc attcttctca tctgctgtaa tagccataag
     1981 tttaaaacct ccttaatttt tagatttctt taactttaat catattcaat cttatctaca
     2041 ttaattttta cctcttttgc ctcaaccccg ctgattttgg gcagctcttc tttcaatttt
     2101 tgttgaataa ggctcgcagt atgaggtata ttgcagtttt tatcaataat caattctaaa
     2161 atggtcttta tatcttcgct cttttgaatt tctattttcg gctttatttt tcttactcct
     2221 tccaccttgt tgagtatatt taaaactatc tgttccaggg aatatgttga tattcttatt
     2281 tctccggaat ctgtaatgac tttaacgtgt ggtaacagtt ggccaatttt ttgatttaac
     2341 catatcagat aaagagcaat aagaaatata aataaaccta ttaaaccaaa aaaagcctga
     2401 taaatggaat tggagtagat taaatataac cattcgttga acaccttaag taaatattct
     2461 atttttatga taccaaaaga aatcaaaaaa cattttaacg aaaa
//
LOCUS       DAOQWT010000218         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_46450_length_2497_cov_2.984848, whole genome shotgun sequence.
ACCESSION   DAOQWT010000218 DAOQWT010000000
VERSION     DAOQWT010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46450_length_2497_cov_2.984848"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PKJ95_04955"
     CDS             <1..901
                     /locus_tag="PKJ95_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNR65625.1"
                     /translation="IRIGDTVLVQKAGEIIPEIVQVIKEKRSGKEKIFQMPDRCPICG
                     SQVVKINEEIVSRCNNLSCPAQVKEKIKHFVSRSAMDMEGLGPALINQLVDKGIIKDF
                     ADLYYLKKEDLINLERMAEKSSHNIIQAIENSKNRPLSSLIYALGIRHIGIYASQLLA
                     EKIDSLFDLEKFSLEDLVGIEGIGPIMAESILLFFKQKENIDIIKKLQQAEVNFLAQK
                     KAVEEGSLKGLQFVLTGTLEHFTREEARDLIEKLGGRVSSSVSKKTDFLLLGRDPGSK
                     YQKAKDLNIKIIEEDDFKKMLKS"
     gene            923..1225
                     /gene="gatC"
                     /locus_tag="PKJ95_04960"
     CDS             923..1225
                     /gene="gatC"
                     /locus_tag="PKJ95_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNR65626.1"
                     /translation="MVIDEKMATVKEIGEVARSAKLELSEEQLKLLSEQLNNTFSFFA
                     KTDKWDAEKIQFDYHKNLTSLEHYHEDIVKPSLPQDTVLGMTRCKQNGYFQVPGIL"
     gene            1280..>2497
                     /gene="gatA"
                     /locus_tag="PKJ95_04965"
     CDS             1280..>2497
                     /gene="gatA"
                     /locus_tag="PKJ95_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNR65627.1"
                     /translation="MQLNELKAVDYLNMVKSREISVEEVVRDIFQQIDRIEHTLKAYL
                     FINLEEALSEAKKMDRRIKDNDIPPLAGLPVAIKDNICIKGMETTCASRILKGFIPPY
                     DATVTSRIKDAGAIIIGKTNLDEFSMGSTTKNSAFQVTLNPWDINRVPGGSSGGSAVT
                     VASHETLIALGTDTGGSVRVPASFCGIVGIKPTYGRISRYGVISSSSSLDQVGILSKG
                     VKDTAMLMNIISGYDPLDSTSSHISVPDFLLSCTGEGIKDLKFAVPKEFFKQELNIEV
                     KERVADSLNKILKLGGEIEEISLPNLEYALSAYSIIAMAEASSNLAKFDGIQYGLRME
                     GSGDLEDMYLKTRSEGFGSEVKRRLILGTYFLSSGHYEDYYLKAKKIRNLIRKDFENI
                     FQKYDLIIGPTTPI"
BASE COUNT          854 a          388 c          532 g          723 t
ORIGIN      
        1 tataagaatc ggtgataccg tactggtcca gaaagccggt gaaataattc ctgagattgt
       61 tcaggtgatt aaagaaaaaa gaagtggaaa agaaaagatt tttcagatgc ctgaccgctg
      121 tcctatttgc ggttcacagg tcgtaaaaat aaatgaagaa attgtttctc gctgtaataa
      181 cctttcctgt cccgcccagg tcaaggagaa gattaaacat tttgtctctc gcagtgcaat
      241 ggatatggaa ggattgggac cagctcttat caatcaactg gtagataagg gaatcattaa
      301 agattttgct gatttatact acctaaaaaa agaagacctt ataaatctgg aaaggatggc
      361 tgagaaatca tcccataata ttattcaggc tattgaaaat agtaagaata ggccgctctc
      421 cagtttaatt tatgcccttg gtattcggca tataggtatt tatgcctctc aattattggc
      481 agaaaagatc gacagtcttt ttgatttgga gaaatttagt ttagaagatt tggttggtat
      541 tgaaggcatt ggtcctatta tggcagagag tattctccta ttctttaagc aaaaggagaa
      601 tatagatatt attaaaaaat tacaacaggc agaggttaat tttttagccc aaaaaaaggc
      661 agtcgaggaa ggcagtttga aaggactgca gtttgtttta acgggaactt tagaacactt
      721 taccagagaa gaagccagag acttgattga aaaattagga ggccgggtat ccagcagcgt
      781 ttccaaaaaa acggatttcc tcttattggg gcgggaccct ggctcaaaat atcaaaaagc
      841 aaaagattta aacattaaga taattgaaga agacgatttt aaaaaaatgt tgaaaagcta
      901 atgtagaaag gaccaattgg taatggtaat agacgagaaa atggcaactg taaaagaaat
      961 tggagaagta gccaggagtg ccaaactgga attgtctgag gagcagttaa aattgctttc
     1021 tgaacaacta aataatacct tctctttttt tgctaaaaca gataaatggg atgctgaaaa
     1081 gatacaattc gattatcata aaaacttaac ctctttggag cactatcatg aagatattgt
     1141 taaaccttct ttacctcagg atacagttct gggaatgacc agatgtaaac agaatggtta
     1201 ttttcaagtt cccggtattt tgtaatgagg gagataaaaa agattattaa atttttgatg
     1261 gaaggcggag gatataattt tgcaattaaa tgaactaaaa gcagttgatt acctgaatat
     1321 ggtaaaaagt agagagatta gtgtagaaga agtggtcaga gacatttttc agcaaatcga
     1381 tagaattgaa cataccctga aagcctattt atttattaat ctagaagagg ctttgtcaga
     1441 agcaaagaag atggaccgga gaataaaaga taatgatata ccacctttag ccggcttgcc
     1501 tgtagctata aaagataata tttgcatcaa aggaatggaa accacctgtg cctccagaat
     1561 attgaaagga tttatacctc cctatgatgc aacagttacc agcaggataa aagatgcagg
     1621 agctattatc ataggtaaaa ccaatctcga tgaattctct atgggctcca ccactaaaaa
     1681 ttctgccttt caggttaccc ttaacccctg ggacataaac agggttcctg gtggatccag
     1741 tggtggttcg gcggtgacag ttgcttccca tgagacatta attgccctgg gcactgatac
     1801 cggtggctca gttagagtac cggcctcttt ttgtggtatt gtgggcatta aacccactta
     1861 tgggaggata tcccgctatg gagttatttc aagttcttct tctctggacc aggtaggcat
     1921 cttaagtaaa ggggttaaag atacagctat gttgatgaat attatttctg gttatgaccc
     1981 tcttgattca acctcatccc atatttctgt tcctgatttc ctgttatcct gtacaggaga
     2041 aggtatcaaa gatttaaaat ttgctgtacc aaaagaattt tttaagcagg aattaaacat
     2101 cgaggtaaaa gaaagagtag cagactctct taataaaatc ttaaaactgg gaggagaaat
     2161 agaagagatt tccttgccca atttggaata tgccttatca gcatactcca taatagctat
     2221 ggcagaggcg agttctaatt tagctaagtt tgatggaata caatatggtt taaggatgga
     2281 gggcagtggt gatttagaag atatgtatct aaaaacaaga agtgaaggtt ttggttctga
     2341 ggtgaagaga aggttaatcc tcggcactta ttttttaagt tcaggacact acgaagacta
     2401 ttatttaaaa gcaaagaaaa ttagaaatct catcaggaaa gattttgaaa atatttttca
     2461 aaaatatgat ttaataattg ggcctactac cccaatt
//
LOCUS       DAOQWT010000219         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_46663_length_2490_cov_2.340452, whole genome shotgun sequence.
ACCESSION   DAOQWT010000219 DAOQWT010000000
VERSION     DAOQWT010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46663_length_2490_cov_2.340452"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PKJ95_04970"
     CDS             <1..194
                     /locus_tag="PKJ95_04970"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNR65628.1"
                     /translation="MDFLGKSLKSQMRTANKLQVPYVLILGSEELKENKVTIKDMVNG
                     VQEEISLNDILAYLKKAYF"
     gene            263..1558
                     /gene="asnS"
                     /locus_tag="PKJ95_04975"
     CDS             263..1558
                     /gene="asnS"
                     /locus_tag="PKJ95_04975"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNR65629.1"
                     /translation="MKEITISEVGNYDTELVTIKGWLYNQRSSGKVKFMIIRDGTGFL
                     QGILVKSEIEEKYFNLLDGLNHESSIIVGGIIKKEQRAPSGYEMLVKTIEVVQNAEPD
                     YPISLKKHGVAFLMNYRHLWIRTERQNAILRIRTEVVKTIHQFLQKKGFFLIESPILT
                     PAACEGTTTLFETDYFGDKAYLTQSGQLYNEAAAMAFGKVYCFGPTFRAEKSKTRRHL
                     MEFWMAEPEAAYFDFQDNNHLQEEMIVEIFSHVLHHCSLELKILERDISALEKIKSPF
                     PYLSYDDAVKLIQKEGSATQWGDDLTGEDETIISKYYDKPVFVHHFPTKCKAFYMKPD
                     PVNPETVLGADLIAPEGFGEIIGGGERIDDYQLIKERIREHKLPEKDYQWYIDLRKFG
                     SVPHSGFGLGVERTVAWICNLSHIRETIPFPRMLNKIYP"
     gene            1676..1918
                     /locus_tag="PKJ95_04980"
     CDS             1676..1918
                     /locus_tag="PKJ95_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65630.1"
                     /translation="MVFSKRQKKCKYKDVGISEGDFPYPLQELFLPNNEKVFLLSLSG
                     AFLLCHCEGTPPSVIESERRERSNLILSNPNQKSSK"
     gene            1984..>2490
                     /locus_tag="PKJ95_04985"
     CDS             1984..>2490
                     /locus_tag="PKJ95_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNR65631.1"
                     /translation="MILPEQSKIDPKAWKRTHSCGELNKNNIGQTVTLMGWVNTYRDH
                     GGLIFVDLRDREGKTQVVFDPEISEEAHLMAHKIRNEFVLAVKGEVISRGEGAFNPNL
                     KTGEIEVRVNQMKILNQSEPPPFNIDDHGEIGDNVRLKYRYLDLRRKEMKKNIILRHK
                     VCLLIRNFL"
BASE COUNT          862 a          386 c          531 g          711 t
ORIGIN      
        1 aaatggattt tctgggaaaa agtttgaaat ctcagatgag aacggctaac aaattacagg
       61 tgccatacgt attaatctta ggttccgaag aactaaagga aaacaaagta accataaaag
      121 atatggtaaa tggtgtgcag gaagaaatat ctttaaatga tattttagcc tacctgaaaa
      181 aggcatattt ttaaagaaac ttaaaataat gaatcattat tttattaaat tgtctgttta
      241 taaataagga ggaagaatcc ggatgaaaga gattactata tcagaggttg ggaattatga
      301 tacagaattg gtaaccatca agggatggct ttataaccag cgctccagtg gcaaggtaaa
      361 atttatgatt atcagagatg gtactggttt cctgcaggga atactggtta aaagcgaaat
      421 tgaagagaaa tattttaatc tacttgatgg attaaaccat gaatcttcca ttatagtggg
      481 ggggattata aaaaaagaac aaagagcgcc aagcggatat gaaatgcttg ttaaaaccat
      541 tgaagtagtt caaaatgccg agcctgatta ccccatctct ttaaaaaagc acggggtggc
      601 ttttctaatg aattaccggc atctctggat aagaacagaa agacagaatg caattttaag
      661 aattagaacg gaagttgtga aaaccatcca tcagttttta cagaaaaaag gttttttcct
      721 tattgaatca cccatactga cacctgctgc ctgtgaaggt accaccaccc tctttgaaac
      781 agactatttt ggcgataaag catatttaac ccagagtgga caactttata atgaggcggc
      841 tgctatggct tttggcaaag tctattgttt tggtcccact tttagagctg aaaagtctaa
      901 aacgagaagg catctgatgg agttctggat ggctgaaccg gaggcggcct attttgattt
      961 tcaggacaat aatcatttac aggaagaaat gattgtggag atattttccc atgtattgca
     1021 ccattgctca ctggagttaa aaatactgga aagggatatc tctgctttag aaaagataaa
     1081 gtctcctttc ccctatttat cttatgatga tgctgttaaa ttaattcaaa aagaggggag
     1141 cgcaactcag tggggagacg acctgaccgg tgaggatgag actattattt ctaaatatta
     1201 tgataaaccg gtctttgttc accattttcc tactaaatgc aaggcttttt atatgaaacc
     1261 agacccggtg aatccagaaa cagttttggg ggctgacttg attgccccag aaggatttgg
     1321 cgagataatc ggagggggag agcgtattga cgattatcag cttattaaag agaggataag
     1381 ggaacataaa ttacccgaga aagattacca atggtatatt gatttgagaa aatttggttc
     1441 tgtaccacat tctggttttg ggctgggagt ggaaagaacc gttgcctgga tatgtaatct
     1501 ttcccatatc agagaaacaa taccttttcc cagaatgtta aataaaatct atccttaaat
     1561 tcattaagtt taataaaaca gtcgtttgtt ggtcgttggt atttagtatt tagttaaaat
     1621 gcaaatgtca agcagcgggg ataagcgaaa gaatagagtt tatagttaat agtttttggt
     1681 ttttagtaaa agacaaaaaa aatgcaaata caaagatgtg gggataagtg agggagattt
     1741 cccctatcca ttgcaagagc tttttctccc taacaatgag aaggtgtttc tcttgtcatt
     1801 gtcaggagcc tttttacttt gtcattgtga aggcacccct ccttcagtca ttgagagcga
     1861 acgtagagag cgaagcaatc tcatactctc caacccgaat cagaagagta gtaaatgaga
     1921 gaacgaaaaa tggctttatt cattttatat taaaaattat atttataagg agtgaaccaa
     1981 aaaatgattt tacctgaaca aagcaaaatt gacccaaaag cctggaaaag gactcatagc
     2041 tgtggtgaat tgaacaaaaa taatattggt caaacagtaa cactgatggg ctgggtaaat
     2101 acttatcggg atcatggtgg tctcatcttt gttgatttac gggatagaga aggaaaaacg
     2161 caggtagtat ttgacccgga aatttctgaa gaggcacacc tgatggcaca caaaatacga
     2221 aacgaatttg ttttagccgt caaaggagaa gtaatatctc ggggagaagg tgcttttaat
     2281 cccaatttaa aaactggtga aattgaagta agggtgaatc agatgaaaat tctaaaccaa
     2341 tctgaaccac ccccctttaa tatagatgac catggtgaaa ttggagataa tgtacgcttg
     2401 aaatatcgct acctggactt aagaagaaaa gaaatgaaaa aaaatattat tttacgtcat
     2461 aaagtttgcc tattaatcag aaacttcttg
//
LOCUS       DAOQWT010000220         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_46678_length_2489_cov_2.826212, whole genome shotgun sequence.
ACCESSION   DAOQWT010000220 DAOQWT010000000
VERSION     DAOQWT010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46678_length_2489_cov_2.826212"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PKJ95_04990"
     CDS             <1..606
                     /locus_tag="PKJ95_04990"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNR65632.1"
                     /translation="GTLVDSDYNDLIWHKAIPEMVAEKNGLNFEEAAAKVKKEYDRVG
                     EDDYRWYDIKYWISFFQLAIGYQELLQKYEQNIIIFSDVVPTLEQLCRKYQIISITSM
                     PWEFMNIKLKRIKKYFQENFSTLSEYKELKNKDAFFQISRKLRVNPEQILHIGDHEKQ
                     DFFAAREAGWKALLIDRFYTGLQEKYPDSVINTLTELPDRL"
     gene            649..1491
                     /gene="mutM"
                     /locus_tag="PKJ95_04995"
     CDS             649..1491
                     /gene="mutM"
                     /locus_tag="PKJ95_04995"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HNR65633.1"
                     /translation="MPELPEVETIKRDLEKEILYKRITDIRVCLPRIIKLPGLAQFST
                     GLKGKFIKGVKRRGKYILCILNNDGYLIFHLGMSGVLFYQRKNENISTRIKEKHNHLL
                     FFFEDGSKMIYNDVRQFGKIWLIHANEKLPEIESLGWEPLEDSFTFQEFFRSVQNKKG
                     NIKSLLMNQKNIAGIGNIYASEILFLAGIHPLRKADSLTENELRKLFLSIRSTLNKAV
                     LARGMTMEDESYFDLLGKPGNYREEVLIYGKKKGNCPTCGHPLSVIRIENRSTYLCPN
                     CQKN"
     gene            1554..>2489
                     /gene="rnz"
                     /locus_tag="PKJ95_05000"
     CDS             1554..>2489
                     /gene="rnz"
                     /locus_tag="PKJ95_05000"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNR65634.1"
                     /translation="MDKIVFLGTSAGMPTQERNVPALAMIFKSGSEFWLFDCGEGTQR
                     QILQANLKLSRLSNIFVSHLHGDHTFGLPGLLATRGLLGVKKPLNIFGPVGLDVFLKN
                     NLDYSYTHIPYPYHFYPIEKTKFLTPHLLWEQGNIAIYCGVLNHNIDSFGFAIIEKNI
                     KKNILREKLLDLAIPPGPVYKKFKESEMINLEDGRTLASADFVKETVTTKKVCYCGDT
                     AFSQNAIVLSEGADILIHEATFSVQSKEKAIRSFHSTVEDALRVARLARVKKLVLVHI
                     SPRYNDVSGNFDNYAQFSGIVSPGDPEIILAEDFME"
BASE COUNT          853 a          405 c          511 g          720 t
ORIGIN      
        1 ggaacactgg tagattctga ctataatgat ttaatctggc ataaagctat accagagatg
       61 gtagctgaaa aaaatggctt aaattttgaa gaagcggctg caaaagtaaa aaaagagtat
      121 gacagggtcg gagaagatga ttatcgatgg tatgatataa aatattggat tagttttttc
      181 cagcttgcta ttggttatca ggaattatta caaaaatacg aacagaatat tattatattt
      241 tcagatgttg ttccaaccct ggagcagtta tgcaggaaat accagataat aagtatcacc
      301 agcatgccct gggaatttat gaacataaag ttgaagagaa ttaaaaaata ctttcaggaa
      361 aacttttcta ccctatcaga atataaagaa ctcaagaata aagatgcctt tttccaaata
      421 agccgtaaat taagagtaaa ccccgaacaa atacttcata ttggtgacca tgaaaagcaa
      481 gacttctttg ctgcccgcga ggcaggatgg aaagcactgt taattgacag attttacaca
      541 ggacttcaag aaaaatatcc cgattcagtt attaacactc ttaccgaact tcccgataga
      601 ttatagtagt aataccaata ccctttttat gagattatga ggtaaattat gccggaatta
      661 ccagaagttg agaccataaa aagagattta gaaaaagaga tattatataa aagaatcacc
      721 gatatccggg tctgccttcc ccgaatcata aaacttcccg gtttagctca attcagcaca
      781 ggtttgaagg gaaaatttat taaaggggta aaaagaaggg gtaagtatat tctctgtatt
      841 cttaataacg atggttacct tatctttcat ctgggaatga gtggagtgct cttctatcag
      901 agaaagaatg aaaatatttc cacaagaatc aaagaaaaac acaaccacct tcttttcttt
      961 tttgaagatg gcagtaagat gatatacaat gatgtaaggc agtttggtaa gatctggcta
     1021 atccatgcta atgagaaact acccgaaatt gaatcattgg gatgggagcc cctggaagat
     1081 agctttactt ttcaggagtt ttttagatca gttcagaata aaaaagggaa tataaagtca
     1141 ttattgatga accaaaaaaa tatcgctgga attgggaata tctacgccag tgaaatacta
     1201 tttttggccg gtattcaccc tctgcgcaag gcagattcgt taactgaaaa tgagttaaga
     1261 aaattattcc tttccattag aagtaccctg aacaaagcag tgctagcccg aggaatgacc
     1321 atggaggatg aatcttactt tgatttactg gggaagccgg gcaattatag agaagaagtt
     1381 ttgatttatg gtaaaaagaa aggtaattgt ccaacctgtg gccatccttt gtcggtaata
     1441 aggatcgaaa acagaagtac ttatctgtgc ccgaactgtc aaaaaaacta aaccggtaaa
     1501 tgagagaggg aagagtaaga gaatgaatag tattagtata agaaaaatat agtatggata
     1561 agattgtttt tttaggaaca agcgctggta tgcccaccca agaaagaaat gtacccgctt
     1621 tagcgatgat ttttaaatca ggcagtgaat tctggttatt tgattgcggg gaaggaacac
     1681 agcggcagat tcttcaagct aacctcaaac tctccagatt gagtaacatc tttgtcagcc
     1741 atcttcatgg cgatcatacc tttggtcttc caggattgct ggctaccaga ggtttattgg
     1801 gtgtcaaaaa acctctcaat atcttcggac cggttggttt agatgttttt ttaaagaaca
     1861 atctggatta ttcttatacc catataccat atccctacca cttttatccc attgagaaga
     1921 caaaattcct tactccccat ctgctttggg aacaggggaa tattgctatc tattgtgggg
     1981 tacttaatca taatattgat agttttggat ttgccattat cgaaaagaat attaaaaaaa
     2041 atattttgag ggaaaaattg ttagatctgg caattccgcc aggaccggtt tataagaaat
     2101 tcaaagagag tgaaatgata aaccttgaag atggaaggac tcttgcctct gctgattttg
     2161 taaaagaaac agttacaact aaaaaggtat gttactgtgg agatactgcc ttttcacaaa
     2221 atgcaattgt tctttcagag ggtgcagata tacttatcca tgaagccact ttttcggtcc
     2281 aaagtaagga aaaagctatt aggagttttc attccacagt tgaagatgcc cttagggtag
     2341 ccagattggc aagggtgaaa aaattggtcc tggtacatat ttctccccgc tataatgatg
     2401 tatcaggaaa tttcgataat tatgctcaat ttagtgggat agtcagtccg ggtgatccgg
     2461 aaattatact ggcagaagat tttatggag
//
LOCUS       DAOQWT010000221         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_46710_length_2488_cov_3.110152, whole genome shotgun sequence.
ACCESSION   DAOQWT010000221 DAOQWT010000000
VERSION     DAOQWT010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46710_length_2488_cov_3.110152"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKJ95_05005"
     CDS             <1..328
                     /locus_tag="PKJ95_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65635.1"
                     /translation="AQIREMMLTFQKETLELRQQIQTKQLELRELRLSSDPDLNQVKA
                     KFEEISKLQLEIRMKAFERQQKVKEILTPEQLENCRQGLQMQRFGSEMGRFNYNPGRG
                     CRGNCR"
     gene            complement(448..927)
                     /locus_tag="PKJ95_05010"
     CDS             complement(448..927)
                     /locus_tag="PKJ95_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65636.1"
                     /translation="MINKHSLEVGLSFGITSGIITTLGLMVGLHSGTHSKVTVIGGIL
                     TIAIADSFSDALGIHISEESEGIHSDKEIWQSTISTFLAKFIFTSLFIIPILIFELST
                     AIIISVVSGLFLLSILSFILSKKQGTKGWGVVLEHLVVAIIVIFLAHYAGVWIASIF"
     gene            complement(994..1314)
                     /locus_tag="PKJ95_05015"
     CDS             complement(994..1314)
                     /locus_tag="PKJ95_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNR65637.1"
                     /translation="MRLLSQYFPEFANILDEIDNIYREKRLIDEKTYQFICLALSIKN
                     RSAPCVKKHFKGALEAGATLKEISYIMALTFRESAGSDDCWVHDVLKDYKEIIKNNIK
                     CCDD"
     gene            complement(1370..1603)
                     /locus_tag="PKJ95_05020"
     CDS             complement(1370..1603)
                     /locus_tag="PKJ95_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR65638.1"
                     /translation="MIIEILGTGCPKCKKLFENVQQAVKELGIEAEIIKTEKLDKITE
                     YGVMVMPALVIDNEIFSPGKVLSAEDIKKIIKS"
     gene            complement(1636..>2488)
                     /locus_tag="PKJ95_05025"
     CDS             complement(1636..>2488)
                     /locus_tag="PKJ95_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNR65639.1"
                     /translation="KGAGLGPAITFLYSGPAINVLAIILTARILGWQLGFARAIGAVI
                     FSVVIGLSMHFIFLSEGRDKKVNGESKFGVGKSEPDNRTGVQDLLYFISMIGFIIFAN
                     WAKPQTEDENLWALIFQYKWYFAFAFTLLFLYTLFSWFKKQEIISWLEYSWDFTQQII
                     PLLFLGIFFAGLLMGRPGEEGLIPSRFIASLVGGNSFLANFFASFLGAFMYFATLTEI
                     PIIQGLLGSGMGQGPALALLLAGPALSLPSMLVIRSVIGTKKTIVYVALVIIMATISG
                     MFYGNII"
BASE COUNT          863 a          494 c          376 g          755 t
ORIGIN      
        1 ggctcaaatt agagaaatga tgcttacctt ccagaaagag actttagaac tcaggcagca
       61 gattcaaacc aagcaattgg aattaagaga gttaaggtta tcgtcagacc cagatttaaa
      121 ccaggtgaaa gcaaaattcg aggagatttc taaactacaa ttagaaatca gaatgaaagc
      181 atttgaaagg caacagaagg taaaagaaat tcttaccccc gaacagctgg aaaattgcag
      241 acagggtctt cagatgcaga gatttggctc agaaatgggc agatttaatt ataacccggg
      301 cagaggatgc aggggaaact gcaggtaagc agacttgtta aaaagcccgt aatcaaaaaa
      361 gagagaagat tctacccttc tctctttttt gattaagata agaaaaaagt gccaatactt
      421 cgataaaaaa cgaacagcta aaaattatta aaaaattgag gctatccaaa cacctgcata
      481 atgagctaaa aatattacta ttatggcaac aacaagatgt tccaatacta ccccccaccc
      541 ttttgtaccc tgcttttttg ataatataaa actgagtatt gataacaaaa acagccctga
      601 tactacacta ataattattg cagtggaaag ttcaaaaatt agaattggta ttataaatag
      661 agaggtaaaa ataaacttag ctaaaaaagt agatatcgtt gattgccata tttctttgtc
      721 actatgtatc ccctctgatt cttcagatat atggattccc agagcatctg aaaaagaatc
      781 tgctatagct atggtcaata tgccgccaat aacggtaact ttggaatgtg tccccgaatg
      841 taatccaacc atcagaccaa gtgtagttat aatacctgaa gtaatcccaa aagataaacc
      901 gacttctaag gaatgtttat ttatcatatt aacctttcat atattttcat atataataaa
      961 attttattat ataacaatga tagattattt tatctagtca tcgcaacatt ttatattatt
     1021 tttaattatt tctttatagt cctttaatac gtcatgaacc caacaatcat cacttccagc
     1081 actctctctg aaagttaaag ccatgatata actaatttct tttaaagttg ctccagcctc
     1141 taatgctcct ttaaagtgct ttttaacaca gggtgcagaa cgatttttaa tagacagggc
     1201 taaacaaata aactgatagg ttttttcatc aatcaatctt ttctctcgat atatattatc
     1261 aatttcatct aatatattgg caaattctgg aaaatactga gataatagcc tcatcataaa
     1321 cctccattgt attaattaat tacaatttat ataatatttt ttcacaattt tatgatttta
     1381 ttattttttt aatgtcctct gctgacaaaa ctttgcctgg gctaaatatt tcattatcaa
     1441 tgactaaagc cggcatcacc attacaccat attctgttat cttatcaagt ttttctgttt
     1501 taatgatttc tgcttctatt ccgagctctt taactgcctg ttgtacattc tcaaataatt
     1561 ttttgcattt gggacagccc gtccctaata tttcaattat cataatttat ctccttttgt
     1621 tttgattcta taaactcaaa ttatatttcc atagaacatt ccacttatag tagccatgat
     1681 aatgaccagt gctacataca ctatagtctt tttagtccct attacactgc ggattaccag
     1741 catactgggc aaacttaaag caggcccagc taagagcaat gctaatgccg gcccctgtcc
     1801 catgcctgaa ccgaggaggc cttgaatgat cggtatttca gtgagtgtgg caaaatacat
     1861 aaaagcaccc aaaaaagagg caaagaaatt tgctaaaaac gaatttcccc ctaccaggga
     1921 tgcaataaaa cgagatggga ttaacccttc ctctcctggt ctacccatca gaagtccggc
     1981 aaaaaagata cctaaaaaaa gtaaaggtat tatttgctga gtaaagtccc atgaatattc
     2041 caaccaggaa ataatctcct gttttttaaa ccaactaaac agggtataga gaaacaacaa
     2101 agtaaaggca aatgcaaaat accatttata ttgaaatatt aatgcccata gattctcatc
     2161 ttctgtctga ggtttagccc agttggcaaa gattataaaa ccaatcatag aaataaaata
     2221 aagcaaatcc tgtaccccgg ttctattatc tggctcactt ttgccaaccc caaattttga
     2281 ttctccattt acctttttat ctcttccctc agataaaaag ataaaatgca tacttaagcc
     2341 tatgactaca ctgaatatca ctgcacctat tgctctggcg aaacccaatt gccagcccag
     2401 tatccgggca gtcaaaatta tagcaagcac atttatggcc ggacctgaat aaagaaaagt
     2461 tatcgccggt cccagccctg cccctttc
//
LOCUS       DAOQWT010000222         2471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_47234_length_2471_cov_2.977649, whole genome shotgun sequence.
ACCESSION   DAOQWT010000222 DAOQWT010000000
VERSION     DAOQWT010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47234_length_2471_cov_2.977649"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKJ95_05030"
     CDS             <1..289
                     /locus_tag="PKJ95_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HNR65640.1"
                     /translation="AREGGYDYKVFKIPMSWVARAIETGETAGIMKAIVNSKNNQILG
                     AAILGLEGGEVMAILQVAMLAGLPYTVLRDAIFSHPSISEALNILFEESTD"
     gene            329..580
                     /locus_tag="PKJ95_05035"
     CDS             329..580
                     /locus_tag="PKJ95_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65641.1"
                     /translation="MLSLRKKTIFKEEVSDMEKTGYAILIIAAIAWIIAWMVGMFQNT
                     WLGIIGLATMVGLGLLFIKALSDRVKSSKNDHYSRDVEK"
     gene            597..917
                     /locus_tag="PKJ95_05040"
     CDS             597..917
                     /locus_tag="PKJ95_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HNR65642.1"
                     /translation="MLLTTQNQFSEYEIIQTLGLVKGNTVRARHLGKDMMATFRNMVG
                     GEIEEYTKLLAESRDQATDRMIMNAKMLGADGIVGIRFTTSSIMQAASEILVYGTAVK
                     FKKK"
     gene            1274..>2471
                     /locus_tag="PKJ95_05045"
     CDS             1274..>2471
                     /locus_tag="PKJ95_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65643.1"
                     /translation="MLKLLKNLKPYWLSIIGVILFVFLQAISELFLPTLMSNVVDIGV
                     VNGGINYILKIGGMMLFIAFIAMAASITGSYLSSRLSIGFARDLRTMVFTKVESFSLE
                     DFNEKGTDSLIVRTTNDITQIQQLTVTLFRMFLRAPMLFIGGIIMAFSKNAQLAFLLM
                     SILPILSIIIYFVAKKSMSLFQAMQIKVDKLNSVLREYLDGIRVIRAFNREDYEKERF
                     DKSNFDLTGTARKVNTLMALLMPVMILVLNLTIVAIFWLGSIRIGSDIMQVGDLMAFV
                     QYASQIMFSLIMFSMMFVMFPRASVSAMRINEVLNVEPKINDPVIPLIPEQTLLEDGS
                     EKISRNIALQETRAEKNGKGHLKFDKVSFCYQNAKEYAVCDISFQARPGEITAIIGST
                     GSGKSTL"
BASE COUNT          785 a          380 c          517 g          789 t
ORIGIN      
        1 ggcaagagag ggcggatatg attataaagt gttcaaaata cctatgagct gggtggcccg
       61 tgctattgaa acaggagaaa cagcgggtat aatgaaggca attgtcaatt ccaaaaacaa
      121 tcaaattctt ggtgcagcga ttttaggtct ggaaggtggt gaggtaatgg ctattttaca
      181 ggttgctatg cttgccggat taccatatac cgtactccgt gatgctatat tttcacatcc
      241 cagtatttct gaggcattaa atattctatt tgaagaatcg acagattaaa gatggggata
      301 ggaagggtgg taaaactcat tttattgggt gttatcttta agaaagaaga caatatttaa
      361 agaagaggtt agcgatatgg aaaagaccgg ttatgctatt ttaattattg ccgctatagc
      421 ctggataata gcctggatgg tgggaatgtt tcagaatacc tggctgggga ttatcggatt
      481 ggcaacaatg gtaggcctgg gattactttt tattaaagca ttgagtgatc gggtgaaaag
      541 ttccaaaaat gatcattatt ccagagatgt tgagaaataa ggggataaaa atatagatgt
      601 tattaactac tcagaatcaa ttttccgaat atgaaataat tcagaccctt ggactggtta
      661 aaggaaatac agttcgcgca cgccatcttg gcaaagatat gatggctacc tttcgcaata
      721 tggtaggagg tgaaattgaa gaatatacca aattgcttgc cgaatctcgg gaccaggcaa
      781 cagaccgtat gattatgaat gctaaaatgc tgggagcaga tggtatagta ggtattcgat
      841 tcactacttc ctctattatg caggcagcat cggagatttt ggtttatgga actgctgtaa
      901 aatttaagaa aaaataagct gccacaaccg ctatctgtaa cgacattagc tgaagaagaa
      961 cgactggatt agttataatt tgctcaatat ttttgtacat ctgtcttaat attgtcaaaa
     1021 atctctccaa aagactgacc atgagcagag aataaacaga tgaaagtatt gaccggttat
     1081 ttttaagatc catcaaaacc ttccaatcta aaggttttat aggtatttaa catatctttt
     1141 tgctcctttt taatttaaaa aaatttaaat attatagaaa aaaactgatt tatatgagag
     1201 aataatgaat gtatttttta tacaattatt attagtcctc taatctaatt tttttcttca
     1261 ggagaaattt gcattgctca aattattaaa gaacctgaaa ccttactggt tatcgattat
     1321 tggagttatt ctttttgttt ttttacaggc aatttctgag ttatttctgc ccacccttat
     1381 gtccaatgta gtggatatag gagtggttaa tggaggtata aattatattt tgaaaattgg
     1441 tggtatgatg ctcttcattg cctttatagc aatggcagcc tctatcaccg gaagctatct
     1501 ctcttcacgg ttgtctattg gttttgccag ggatctcagg actatggttt ttacaaaggt
     1561 agagagtttt tctttagaag attttaatga aaaggggacc gattctttaa tagtcagaac
     1621 caccaatgat ataacccaga ttcaacaatt aactgttact ctattcagaa tgtttctcag
     1681 agcccccatg ctttttatag gcggcatcat catggctttc tcgaaaaatg cccaactggc
     1741 ttttctttta atgagtatat tgcccatcct atctatcata atctattttg tggcaaagaa
     1801 gagtatgtct ctatttcaag caatgcaaat caaggtagat aagcttaact cggtattgcg
     1861 ggaatattta gatggtatca gggtaataag ggcttttaac agggaggatt atgaaaaaga
     1921 acgctttgat aaaagcaatt tcgatttaac agggactgcc agaaaagtta atacacttat
     1981 ggctttgttg atgcctgtga tgatactggt tctcaatctg acaattgtag caatcttctg
     2041 gttaggaagt ataagaatcg gaagtgatat tatgcaggtg ggagacctga tggcttttgt
     2101 gcaatatgcc tcccagataa tgttttcttt aattatgttt tctatgatgt ttgtaatgtt
     2161 ccccagggct tcagtttccg ccatgagaat taatgaagtt ttaaatgttg aaccaaaaat
     2221 taatgacccg gtaataccgt taataccgga acaaactcta ttagaagatg gaagtgaaaa
     2281 aattagtaga aatattgcct tacaggaaac cagagcagaa aaaaatggga aagggcatct
     2341 gaaatttgat aaggtatctt tctgttatca gaatgctaaa gaatatgccg tttgcgatat
     2401 ttcttttcaa gccagaccgg gagaaatcac tgctattata ggaagtaccg gctctggtaa
     2461 gtcgactctg a
//
LOCUS       DAOQWT010000223         2465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_47422_length_2465_cov_2.681743, whole genome shotgun sequence.
ACCESSION   DAOQWT010000223 DAOQWT010000000
VERSION     DAOQWT010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47422_length_2465_cov_2.681743"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKJ95_05050"
     CDS             complement(<1..453)
                     /locus_tag="PKJ95_05050"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65644.1"
                     /translation="MKEPKFLIVSNSLRLVIGVLLSALSGVAFMLAFPPYEIWFLVFI
                     GWIPLQIAQFRIIPKRVSSIASATALFVWLQGYIGPIFASFKTFMIWLPLVVGLLSLL
                     MDAKLRKFHHNTRYRWYILSGVASWAGTEMIRLFLPIAGTWAFIAYPLY"
     gene            complement(679..773)
                     /locus_tag="PKJ95_05055"
     tRNA            complement(679..773)
                     /locus_tag="PKJ95_05055"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(735..737),aa:Ser,seq:cga)
     gene            complement(824..1279)
                     /gene="tadA"
                     /locus_tag="PKJ95_05060"
     CDS             complement(824..1279)
                     /gene="tadA"
                     /locus_tag="PKJ95_05060"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNR65645.1"
                     /translation="MTENINQKMMQIALSLAEQAFFEEEVPVGALIVSGEEVISQAYN
                     QKEILQDPTAHAEILALREACARTGSWHLDDATIYVTLEPCPMCAYALIQARVKRLVF
                     GAYDPKAGAAGSVINIFKKKLFNHEVEVIGGVLEEECGELLKKFFQNRR"
     gene            complement(1307..1380)
                     /locus_tag="PKJ95_05065"
     tRNA            complement(1307..1380)
                     /locus_tag="PKJ95_05065"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1344..1346),aa:Arg,seq:acg)
     gene            1623..2396
                     /locus_tag="PKJ95_05070"
     CDS             1623..2396
                     /locus_tag="PKJ95_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNR65646.1"
                     /translation="MKDTKQIFQKDYEIHSYEVLKDQRLNPVRLVDYLNDIAGQHSES
                     LGYSMKDLFEKDCSWIVLSWNLCISRLPYLRSKIHIETWVSQVKRSFAYREFLLKDDN
                     EIFCKASSLWLFYHITKHRPVKIPLELVNLWILNREISCPESIIESDLFERKDFIYEE
                     FDYTVEKQDIDILNHVHNSRYIGWIINSKPDHLKKDYSLKHLQISYHHEIKYPAQVIV
                     QQKIFPRLNRRQYFIKDEICDREKEIIFSEVVTQWDLSE"
BASE COUNT          792 a          475 c          452 g          746 t
ORIGIN      
        1 atacagcgga tacgcaataa aagcccatgt tccagcgatt ggaaggaata aacggatcat
       61 ctcagtccct gcccagctgg ccaccccgga gaggatgtac caacgatacc tggtattatg
      121 atgaaatttc cgtaacttag catccatcag gagcgataat aaacccacta ccaggggtaa
      181 ccaaatcata aatgttttaa acgaggcaaa tatgggtcca atatatccct gtaaccagac
      241 aaatagagca gtcgctgagg cgatggagga gactcgtttt ggaatgatcc tgaattgagc
      301 tatctggagc gggatccacc caataaatac caggaaccaa atttcataag gcggaaatgc
      361 caacatgaat gcaacaccac tgagagcact taataatacc cctatgacta atcgtagtga
      421 gttggatact atgagaaact ttggttcttt cacattaccc ccccctaaaa tcttaattat
      481 tgggatggag aaattatact aacccttcaa tcatgcttat attatagtct ctataagaat
      541 atgggtaaat aacttttatt atcccatttt aaactcttgg ttgaaatcac cacccggatg
      601 agcagctaaa aactttatac ttataattag aaaagttaat agtacagtta tatcaataaa
      661 caacggggtt ttaatatctg gcggagaggg cgggattcga acccgcgaga cagttattag
      721 ccgcctactc gatttcgagt cgagcgcctt caaccggact cggccacctc tcctgatgat
      781 atctttctgt atctatattt gcatttgcta cgcttttagt ggattatctt ctgttttgaa
      841 agaatttctt caataattct ccacattctt cttctaatac gccaccgatt acttcaactt
      901 cgtgattgaa tagcttcttt ttaaagatgt tgataacact accggcagca cctgccttgg
      961 ggtcataggc accgaaaaca agtctcttta ctcgcgcctg aatgagggca taggcgcaca
     1021 tggggcatgg ctctaaagtg acatagatag tggcatcatc caaatgccag ctacctgtcc
     1081 gggcacatgc ttcacgaagt gccaggattt cggcatgggc ggtggggtct tggagtattt
     1141 ctttttgatt ataagcctga ctgattacct cttctcctga aacaatgaga gctccgacag
     1201 gcacttcttc ttcaaaaaag gcctgctctg ctaaagaaag ggcaatttgc atcatcttct
     1261 gatttatatt ttctgtcatt tttctaatta tttaattaaa aaaaggcgcg cccggaggga
     1321 ttcgaacccc cagtctactg atccgtagtc agttgctcta tccaattaag ccacgggcgc
     1381 ttcacacctg aaggaataaa aaatacgtag catttaagct atgatagatt ttattataat
     1441 ttaagaactc cgtcaaacga aatcagtcaa ttattaaatt ttcttaaatt ctttaagcta
     1501 tgttaaaaat aattatggta atatacgtaa agtacagaaa aataattatg ggtatgttat
     1561 acttttcagt aattgcagcc tatatttaca atgttagtta aaaatacaaa aggttctcat
     1621 ttatgaaaga tacgaaacag atttttcaaa aagattatga gatacattcc tatgaggtgt
     1681 tgaaagatca gaggcttaat ccggtcaggc tagtcgatta tctgaatgat attgccggac
     1741 aacattctga atcattgggc tattccatga aagacctttt tgaaaaagat tgctcctgga
     1801 tagtcctctc ctggaatctc tgtataagta gacttcctta tttaagaagt aaaatccata
     1861 tagaaacctg ggtatctcaa gtaaagcgca gttttgccta ccgggaattc ttacttaaag
     1921 atgataatga gatattctgt aaggcatctt ctctctggct cttttaccat atcaccaaac
     1981 ataggccggt taaaattcct ttagaattag taaatctttg gattctcaat agagagattt
     2041 catgccctga gtctattatt gaatcagact tattcgaaag gaaagacttt atatatgaag
     2101 aatttgatta tactgttgag aagcaagata tagacatatt aaaccatgtg cataattcca
     2161 gatatatagg ctggattata aatagcaaac cggaccattt aaaaaaagat tattctttga
     2221 aacatttaca gatttcctat catcatgaaa ttaaatatcc ggcccaagtt attgtacaac
     2281 agaagatatt tccccggctt aatagaagac aatactttat caaggatgag atatgtgaca
     2341 gggaaaaaga aataattttt tcagaagtcg taactcaatg ggacctttct gaatgattcc
     2401 ctgttcttct attaataaat ataaaactgc tcttttgtct aagagcagtt aatattatta
     2461 tatta
//
LOCUS       DAOQWT010000224         2451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_47840_length_2451_cov_2.508347, whole genome shotgun sequence.
ACCESSION   DAOQWT010000224 DAOQWT010000000
VERSION     DAOQWT010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47840_length_2451_cov_2.508347"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..675
                     /locus_tag="PKJ95_05075"
     CDS             394..675
                     /locus_tag="PKJ95_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNR65647.1"
                     /translation="MPIYDFRCSSCGHKFEYFTFKLNGQQVNCECCGSVANRLISPVG
                     IIFKGSGFYVTDSRKSSTLTSTDSSDAKTDTGAKTDTGSKIDSKVEKAA"
     gene            777..2330
                     /locus_tag="PKJ95_05080"
     CDS             777..2330
                     /locus_tag="PKJ95_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HNR65648.1"
                     /translation="MKERKKVVFKEYDKYQLMLPSDLEDLIPEHHIVKVLNEIIERMD
                     IEFLKEKYPGGGRSSYHPEMMLKVLLYGYTQQIYSSRKLAKALRENVPFMWLSRNNRP
                     DFRTLNRFRSSIMGLEELEEIFTELLDLLQEEGYIKLENYFVDGTKIEGNANKYTFVW
                     KKSTKKYKAMLREKVKALLIKIEEENVKENDEYGDKDLEELGGEEGISSEKIKSKVEE
                     IEKRLKDKRDDKKLKKALKVLKDDYLPRMEKYENQEKLCGDRNSYSKTDPDATFMRMK
                     EDHMKNGQLKAGYNVQIGTENQFIVNYSIHQRAGDTSCFISHCEELKGKVGRLPENII
                     ADAGYGSEENYDYLEKNNIGTYVKYNNFHLEQKKKFKEDRFRVENLYYDSNLDEYICP
                     GLRRLTFIKECKKKTENGYNTTRRIYECKSCAGCSLKACCTKAAGNRKIQISFKLNEY
                     KAKARSNLLSEIGVKLRGKRAIEPETVFGRLKWNWSFKRFLLRGLEKVKIEWGLLSIA
                     HNIVKLATI"
BASE COUNT          980 a          304 c          478 g          689 t
ORIGIN      
        1 ggactatttt gtttttgata tccataaaat atctcctctg tttaaatttg taatttcaca
       61 gtttatagat gataccttaa tgacagataa ctgtttctga ctcagagtat aatagtttca
      121 taacaatcca taattaagaa tgagtgaaaa gtgagtagtg agtagggaat agtgagtaaa
      181 gtcctatctt tttcactttt cactataatt aaattttagt aagttatgaa atactctata
      241 gtagttactg gcatgtatga aattacatgt aattagaatt ataactcaat ttttgtaata
      301 aggtcaaatc caaaaaatac ttgacagaat gtcggtgaag ttttataatt actcgatgaa
      361 aatcattaaa attatctgaa agggggattt tctatgccaa tctatgattt cagatgttct
      421 tcctgtggac ataaatttga atattttact tttaaattaa acgggcagca agtgaactgc
      481 gaatgttgtg gctcagtagc aaatagatta atatcaccag taggtataat ttttaaagga
      541 tctggctttt atgtgacaga ttctcgaaaa tcctctaccc ttaccagtac agacagctcg
      601 gatgcaaaga ctgatacagg tgcaaaaact gacacaggca gtaaaataga cagtaaagta
      661 gaaaaagcag cttaaataaa aatatctgaa aaaaatataa ggggctgtcc caaaagtcaa
      721 aaaactttag ggcagcctct ttacattttt gatataaatg tactacaata gagcttatga
      781 aagagcgtaa aaaagtagta tttaaagaat atgataaata tcaattaatg ttgccaagtg
      841 atcttgaaga tttaataccc gagcatcata tagtaaaagt attaaatgaa ataatagaaa
      901 ggatggatat agaatttcta aaagaaaaat atcccggagg agggcgaagt agttatcatc
      961 ctgaaatgat gctcaaggta ttgttatatg gatatactca gcagatatat tcatcaagga
     1021 aattagcgaa agcgttacga gaaaatgtcc cctttatgtg gttaagccga aacaatcgtc
     1081 cagacttcag gacattaaat agatttcgtt caagcataat gggtttggaa gaattagaag
     1141 aaatttttac agaattatta gatttactcc aggaagaagg ttatataaaa ttagagaatt
     1201 attttgtaga tggaacaaag atagaaggaa atgcaaacaa atacacattc gtatggaaaa
     1261 aatctacgaa gaaatataaa gccatgctgc gtgagaaagt gaaagctctc ctgataaaga
     1321 tagaagaaga aaatgtaaaa gagaatgacg aatatggaga taaagatctt gaagaacttg
     1381 gaggagaaga aggtataagc tctgaaaaaa taaaatcaaa agtggaagaa atagaaaaga
     1441 gattaaaaga taaaagagat gataaaaaat taaagaaggc gttaaaagtt ctaaaagatg
     1501 actatcttcc ccgtatggag aaatacgaaa atcaggagaa attatgtggt gaccgtaaca
     1561 gttactcaaa aacagatccc gatgcgacat ttatgagaat gaaggaagac cacatgaaaa
     1621 atgggcaatt aaaggctgga tataatgttc aaataggaac ggaaaatcaa tttatagtaa
     1681 actacagtat ccatcaacga gcaggagata catcttgttt tatatctcat tgtgaggaat
     1741 taaaagggaa agtaggaagg ttaccagaaa atataatagc cgatgcaggt tatggcagtg
     1801 aggaaaacta tgactatctg gaaaagaata atataggaac ttatgttaaa tataataatt
     1861 ttcatctgga acaaaaaaag aaatttaaag aagatagatt tagagttgaa aatctgtatt
     1921 acgacagtaa ccttgacgag tatatctgcc ctggtttgag aagattaaca tttataaaag
     1981 aatgcaagaa aaagactgaa aatggttata atacaacacg acgtatatat gaatgtaaaa
     2041 gctgtgcagg ctgctcttta aaggcttgtt gcacaaaggc agcaggaaac aggaaaattc
     2101 agataagttt taaactaaat gaatataagg caaaagcaag gagtaacctc ttatctgaaa
     2161 taggtgttaa actcagggga aaaagagcta tagagccaga gactgttttc ggaagactga
     2221 aatggaactg gtcttttaaa agattccttt taagaggtct tgagaaggta aaaattgagt
     2281 ggggtttatt gagtattgca cataatattg taaaattagc taccatatag aggggatata
     2341 gaccaataag gagaaatatt attacattct tgacttaatt ttataccttt ttactgataa
     2401 atgtaaaaag ggctaatccc aaaatacact tttgggacag ccccttatat t
//
LOCUS       DAOQWT010000225         2444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_48069_length_2444_cov_2.963583, whole genome shotgun sequence.
ACCESSION   DAOQWT010000225 DAOQWT010000000
VERSION     DAOQWT010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48069_length_2444_cov_2.963583"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PKJ95_05085"
     CDS             <1..783
                     /locus_tag="PKJ95_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR65649.1"
                     /translation="SFLIEAYRNNIKKNVLVDVGQNHEALLHNMQLLGINPVIIDSIV
                     ITHCHYDHTLGIAAMLKAIGKQDLPVIAHPGLFRLNFIDKPYLRHVGVMQADSRERIE
                     ANGGTLYLTDHPLQIMPGLITTGEVKRQTDFEEVGIALKTIVEGEIVEDDMKDDISLI
                     AIFKGREIAIVTGCSHAGIVNIVKQSIEMTGLRKIKAIIGGLHLVEATMDRIKKTAAA
                     LSEFDIDLVSAGHCTGFNAQAELHNIFKDKFLPLQTGMKFEF"
     gene            1044..1526
                     /locus_tag="PKJ95_05090"
     CDS             1044..1526
                     /locus_tag="PKJ95_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNR65650.1"
                     /translation="MPRGWYGGGPGGWWQRQFRGGGYRITMPRQIILQVLDESKRHLS
                     AEDIYLKVYQIYPTIGLTTVYRTLELLVRMGLVFKFDFGDGRARYELTRGDGNLSHHH
                     HLICTNCGRIIDYDDFIEEELELIKKTRQELEKKYGFEIRNHTLQFYGLCKKCKAIKE
                     "
     gene            1743..2117
                     /locus_tag="PKJ95_05095"
     CDS             1743..2117
                     /locus_tag="PKJ95_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNR65651.1"
                     /translation="MKIVITSTGADLKSQVDPRFGRCAYFILIDTDKDEFEAIENSGA
                     QGMGGVGVQSAQIMINKGVKTVLTGNCGPNAFQTLQADGIEIISGISGTVEEVLEKFK
                     AGGFQSVSGPNVSPHSGIKKKE"
     gene            2172..>2444
                     /locus_tag="PKJ95_05100"
     CDS             2172..>2444
                     /locus_tag="PKJ95_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNR65652.1"
                     /translation="MMKIAISTDNGFVSEHFGRCPAFTIAEIEGGKVLKIEEINNPGH
                     QPGFLPAFLAERGVKYIICGGMGNRAQSLFAEIGITVANARVKSSGS"
BASE COUNT          868 a          330 c          529 g          717 t
ORIGIN      
        1 tcttttttaa ttgaagctta ccggaataat atcaaaaaaa atgtattggt ggatgttgga
       61 cagaatcatg aagcgctgtt acataatatg caattactcg gtattaatcc tgtaatcatt
      121 gattcaattg taataactca ttgccattat gaccatactc tgggaattgc tgctatgtta
      181 aaggctatag ggaaacagga tttacctgta attgctcatc ccggtctttt caggttgaat
      241 tttatagata agccttattt gaggcatgtt ggtgtaatgc aggcagattc ccgggaaagg
      301 atagaagcta atggcgggac tctctactta acagaccatc ctcttcagat aatgcccgga
      361 cttatcacta caggagaagt aaaaaggcag acggattttg aggaggtagg cattgctttg
      421 aagacaatcg ttgaaggtga gattgtggaa gatgatatga aagatgatat atctttgatt
      481 gccatcttta aaggaagaga gatagctata gtaaccggat gcagtcatgc cggtattgta
      541 aatattgtaa aacaatccat agaaatgacc ggtctgagaa aaattaaagc tattattggt
      601 ggactccatc tggtggaggc aacaatggat agaattaaaa aaactgctgc agctttatca
      661 gaatttgata ttgatttagt ttcagcagga cactgcaccg gatttaatgc acaagctgaa
      721 ctccataata tttttaaaga taaattttta ccattacaaa caggtatgaa atttgaattt
      781 taaaataaaa agggtcagac tctaaaatta tagtatatct gtcctcacta aattttaggg
      841 cctgatccta tttaatttta cttcttttaa atggtcaatg ttttcctatt ttaaacaagt
      901 gaaaaaataa tatgatgagg aaaataattt ataaaatcaa gaaataaaat agtaattatt
      961 tctcttacat ttgacaaggg aagctgtttt gtgtattata ttattgaaaa tgattatcaa
     1021 taaggaatga ggagccaaag aatatgccca gagggtggta tggaggaggt cctggaggtt
     1081 ggtggcagag acaattcagg ggaggcggtt atagaatcac aatgcctcga cagattattt
     1141 tacaggtttt agatgaatca aagaggcatt tgagtgctga ggatatctat ttaaaagtat
     1201 atcaaattta tcctaccata ggtttgacca ctgtctatcg aacattggaa ttgttggtca
     1261 ggatgggatt agtttttaag tttgattttg gtgacggcag agcgagatat gagctaacta
     1321 gaggagatgg gaatttgtca caccaccatc atctgatatg taccaattgt gggagaataa
     1381 tagattacga tgattttata gaggaagaat tggagttaat aaagaaaacc agacaggaat
     1441 tagaaaaaaa atatggtttt gagatcagga atcatacact ccaattttat ggattgtgca
     1501 aaaaatgtaa ggcaataaag gagtagtata tattttttat acaatttatt gaaaatgatt
     1561 atcattacat tgaaaatata aaaagaagaa ataatgagag acaatagctg gtaaaccgtt
     1621 aggaaaatct aactaggagc tggtaatttt aaaggattaa gtaacagcta tagcggaata
     1681 aacgaaaaac aataaaaata atgctgagaa aaaaaataat aaaaattaag gaaggaaaat
     1741 atatgaaaat tgtaattacc tcaactggcg cagatttaaa atctcaagta gatcccagat
     1801 tcggaagatg tgcttacttc attttaatag ataccgataa agatgaattc gaggcaatag
     1861 agaatagtgg agcccagggc atgggaggag taggagtaca atcagcccag ataatgatta
     1921 ataagggggt aaagacagtt ctaactggaa attgtggccc caatgccttt cagactcttc
     1981 aggctgacgg aatagaaatt atttccggta tttccggtac agtagaagaa gttttagaaa
     2041 aatttaaagc aggcggtttc cagtcagttt ctggtcccaa tgtttcgccc cattcaggta
     2101 taaaaaagaa agaataaaat atataataga agtaataatt aggttttaag ataaaattta
     2161 gagggaaata tatgatgaag attgctattt ctacagataa tggttttgta tcggaacatt
     2221 ttggaagatg tccagccttt accatcgcgg agattgaggg tggtaaggtt ttaaagatag
     2281 aagaaataaa taaccccgga catcagccgg gatttctacc cgcctttctg gctgaaagag
     2341 gtgtcaaata tattatttgt ggcggtatgg ggaacagggc gcagtcacta tttgctgaaa
     2401 taggaattac cgttgcaaac gcacgtgtca aatcttcggg ttca
//
LOCUS       DAOQWT010000226         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_48103_length_2443_cov_3.365159, whole genome shotgun sequence.
ACCESSION   DAOQWT010000226 DAOQWT010000000
VERSION     DAOQWT010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48103_length_2443_cov_3.365159"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PKJ95_05105"
     CDS             <1..1137
                     /locus_tag="PKJ95_05105"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HNR65653.1"
                     /translation="DYSRNQRHISFAIAYCIWKHWIITADLDFMCNYGAELFLSIAKF
                     AASLSYFDLEDKKYHTRGLMGPDEFHERLPGITEAGFKDNAYSNILIVWILQRALEII
                     SSILPIKQKNVLLKKLEIDYGELKIWAEITRKMNILMDEEGIIAQFDGYFSLPELNWK
                     AYKKKYGNIQRMDRILKAEGKSTNDYKVAKQADTLMLPYLFSLTELENIFQLLGYQFN
                     GDILRKNYDYYVQRTSHGSTLSKVVHCYLSDILGQSGQSWKWYCEVLNSDINDIQGGT
                     TPEGIHTGVMGGSINIALKRYAGVNIINGIINIDPDIPKKWKYIKFKIKYKEIWYSLK
                     IERHKIIATLSTTKLIDEKYQEKILIRQKEYLLDMNRTYTFYLK"
     gene            1150..1527
                     /locus_tag="PKJ95_05110"
     CDS             1150..1527
                     /locus_tag="PKJ95_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR65654.1"
                     /translation="MAKKHILIVDGDVMKAERLKRNLETEDYSVDVVHYGHEALMVLG
                     RKWVDLVISSITLQGAMNGIQLLQEIKKNKELNETPIIIMSSKINLEKSIYDIGATLF
                     VEKPYDVVEIIQKIKDIFPENDK"
     gene            1580..2053
                     /locus_tag="PKJ95_05115"
     CDS             1580..2053
                     /locus_tag="PKJ95_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNR65655.1"
                     /translation="MAENNSLSLTKLMERLKQIKAKDIMTEDVITVRKDDTLTDVARL
                     MITRRISGFPVIENDKIIGIITANDLFLVMDMIKAGEVLKDNGTDYSQPRVSFAMSTE
                     IITVNPEGTLDDIISLMKYRNIHTLPVVSQNKLVGVIGRRDVFRSFYGAIRDLIS"
     gene            complement(2048..>2443)
                     /locus_tag="PKJ95_05120"
     CDS             complement(2048..>2443)
                     /locus_tag="PKJ95_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HNR65656.1"
                     /translation="NLIMAIKFAKYYELTEKDIVFTVFTDSMELYGSRLKELEKQFGL
                     YNDTDAAIAHHRNLMALTTDYMQELTYNDRKRIHNLKYFTWIEQQGKNLDELNAQWYD
                     YENYWGTIRNQVDKIDELIVQFNKKTGLL"
BASE COUNT          884 a          377 c          452 g          730 t
ORIGIN      
        1 gattacagcc gtaatcagag acacatatct tttgccatag catattgtat atggaaacac
       61 tggataataa cagctgacct ggactttatg tgcaactacg gagcagagct ctttctctcc
      121 atagcaaagt ttgcagccag tttatcatat tttgacctag aagataaaaa ataccatacc
      181 agaggtttaa tgggacctga cgagtttcat gaaagacttc ctggaataac tgaagcagga
      241 tttaaagata atgcttacag taacatcctg attgtctgga tcttgcagcg agcacttgaa
      301 ataatatcat caattttgcc aataaaacaa aaaaacgtgt tattaaaaaa attagaaata
      361 gattatggtg aattaaaaat ctgggcagag attaccagaa agatgaacat actgatggat
      421 gaagaaggta ttatcgccca gtttgatggt tatttttctc tgccagaatt aaactggaaa
      481 gcttataaaa agaaatatgg caatattcaa agaatggacc gtatcttaaa agcagaaggc
      541 aaatcgacta atgattacaa agtagcaaaa caggctgata cccttatgct gccctactta
      601 ttttctttaa cagaattaga aaatatattt caactattgg gatatcagtt taacggagat
      661 attttaagaa aaaattatga ttactacgta cagagaacct ctcacggttc tacactcagt
      721 aaagtggttc attgctattt atctgatatt ttgggacaat ccggacagtc ctggaaatgg
      781 tactgtgaag ttcttaattc tgatatcaat gatattcaag gcggcactac cccggaaggg
      841 atacataccg gggtaatggg tggttctata aacattgctc tgaaaagata tgccggtgta
      901 aatattatta atggtataat aaatattgac cctgatatcc ctaaaaaatg gaaatatata
      961 aaatttaaga taaagtacaa ggaaatatgg tattcactga aaatagaaag acataaaatt
     1021 attgctaccc tatccactac taaattaata gatgaaaaat atcaggaaaa aattcttatt
     1081 cgtcaaaaag aatatctgtt ggatatgaat agaacatata ccttttatct taaatagagg
     1141 gaggggtaaa tggcaaaaaa gcatattctt attgtcgacg gggatgtaat gaaggcagaa
     1201 agactgaaaa gaaatctgga gacagaggat tacagtgttg atgtagtaca ctatggtcat
     1261 gaggcattaa tggttttagg aaggaaatgg gttgatttag tcatctcttc cattacactg
     1321 caaggagcaa tgaacggtat tcaattgtta caggagatta aaaagaataa ggagcttaat
     1381 gagactccga ttattattat gagcagtaaa attaatctgg aaaaaagtat ctatgatata
     1441 ggagcaactc tttttgtgga aaaaccatat gatgttgtag agataatcca aaaaattaaa
     1501 gatatttttc ccgaaaatga caaataaatg atgttattca gataaatctt ttaaaataat
     1561 actttctggg gtttaaaaaa tggctgaaaa taattctctt agtctgacta aattaatgga
     1621 acggctgaaa cagataaaag caaaagatat tatgactgaa gatgttatta ccgtgagaaa
     1681 agatgacact ctcaccgatg tagccagatt gatgattacc agaaggatta gcggattccc
     1741 ggttatagaa aatgataaaa taatcggaat aatcactgcc aacgatctat tcctggtaat
     1801 ggatatgatc aaggctggtg aagtattaaa agataatggc actgattatt ctcaaccaag
     1861 agtaagcttc gctatgtcta cagaaattat tactgtgaat cctgaaggaa ccctcgatga
     1921 tatcatctcc ttgatgaaat acagaaacat tcacacccta ccggtcgtaa gccagaataa
     1981 actagtggga gtcattggcc gcagagatgt attcagaagt ttttatggtg caatcagaga
     2041 tttaatatca taataggcct gtttttttat tgaattgaac aataagctca tcaatcttat
     2101 caacctgatt acgaatagtg ccccaataat tctcataatc ataccattgg gcattcaatt
     2161 catccaggtt ttttccctgt tgttctatcc aggtaaagta ttttaaatta tgaatcctct
     2221 ttctatcatt ataggttaat tcctgcatat aatcagtcgt taaagccatt agattacggt
     2281 gatgagcaat ggcggcatct gtatcattat aaaggccgaa ttgtttttct aattccttca
     2341 accttgaacc gtaaagctcc atagaatcag taaatacagt aaaaacgata tccttttctg
     2401 ttagttcata atatttggca aatttaattg ccataatcag gtt
//
LOCUS       DAOQWT010000227         2431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_48464_length_2431_cov_3.817340, whole genome shotgun sequence.
ACCESSION   DAOQWT010000227 DAOQWT010000000
VERSION     DAOQWT010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48464_length_2431_cov_3.817340"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PKJ95_05125"
     CDS             complement(<1..359)
                     /locus_tag="PKJ95_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNR65657.1"
                     /translation="MRYISTRGNIKPVSAIEAIKLGMVPQGGLFVPERIPSISQKQLY
                     SWINLSYDRLAQHIFHFFLAQDFSEQEIAQNVQRVYHKVHFESDEITPLIQLDNRLFI
                     LELFHGPTGAFKDLALQV"
     gene            666..1616
                     /locus_tag="PKJ95_05130"
     CDS             666..1616
                     /locus_tag="PKJ95_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNR65658.1"
                     /translation="MKKALINYFFREQGKKALALANEFSDVEFEVITSKKELMNRLPE
                     AEIMLGTLFNEEMLRIAPRLKWVQSFMAGVDNYPLKEMEKRGIILTSGRGIHSINIPE
                     YVICALIMLARNFPVIAKNQNSKKWVVVEQDEIYDSTLGILGLGAIGSELAKKAHSMG
                     MKIIALKKTFEQIDYVEELYTPDKMDEIFKKSDYIVNLLPYIPETDKIINKKYFELMK
                     PTASFISVGRGKTVNEEDLIEALRTKKIKGLVSDVFYEEPLSEGSPLWEMENVFITPH
                     VAGQSVKYIEKALGIIRHNLGVYLSGEGEYLNRIDYKKGY"
     gene            1734..>2431
                     /locus_tag="PKJ95_05135"
     CDS             1734..>2431
                     /locus_tag="PKJ95_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNR65659.1"
                     /translation="MNVRELSEKYQNEIVALRREFHQNPELSWQEYNTAARIEKELNK
                     IGINDTKRIAGTGVVSSIKGKKGNKVVALRADIDALLVQEENETFYRSKNDGIMHACG
                     HDAHTAMLLGAAKVLWELREQFEGTVKLIFQPAEEVIEGAKKMLEEGVLEGVDSILAI
                     HVWSELLSGKVSLEEGPRFAAGDRFKIAIKGKGSHGAMPHMGIDAIVAASAVVMNLQT
                     IVSREIDPQEPAVI"
BASE COUNT          822 a          340 c          549 g          720 t
ORIGIN      
        1 acctgtaatg ccaggtcttt aaaggcgccg gtaggtccat ggaaaagttc caggataaag
       61 agtcggttgt caagctgaat caggggggtt atttcatctg attcaaaatg aactttgtgg
      121 tagactcgtt gaacattttg ggctatctcc tgttcagaga agtcctgtgc taagaaaaaa
      181 tgaaagatat gctgagccag ccggtcataa gatagattaa tccagctgta aagctgcttt
      241 tgtgaaattg atggaattct ttccggcaca aacaatccac cctgtggcac cattcccaat
      301 ttaattgctt caatggcaga aactggttta atatttcccc tggtacttat atatctcatt
      361 attctcccca tgggcttcct ggttcttatc tgtatttaaa aatattgaat agcaaaagaa
      421 aaaattttca atcaattcaa aatcaggttt caattatgcc tctcattata gtaaaaagat
      481 tcaaactgaa caagaaaaaa attgtactta acattttaaa tattataaag aattgttcta
      541 agggtatctt tctatagtga gcagtgtcct tcggtgagag gctcttgcaa atttgctttt
      601 attgacttac tatttattaa tggagttact ttgaaaaggt aagaaatctt tatgaggtga
      661 ttgtagtgaa aaaagcatta atcaattatt ttttcagaga gcaggggaag aaagcacttg
      721 ccctggctaa tgaattttcg gatgttgaat tcgaagttat taccagcaaa aaagaattga
      781 tgaatagatt gcccgaggca gaaataatgc tgggaacact ttttaatgag gagatgctaa
      841 gaattgctcc caggttgaaa tgggttcaat cttttatggc cggagttgat aactatccct
      901 taaaagaaat ggagaagagg ggcattatac tgaccagtgg aaggggaatt catagtatta
      961 atattcctga gtatgtgatt tgtgctttaa ttatgcttgc ccgaaatttt ccggttatag
     1021 ccaaaaacca gaacagtaag aaatgggttg tagttgaaca ggatgaaatc tatgactcta
     1081 ccctgggaat actgggattg ggcgctattg gttcagaact ggctaaaaaa gcacactcaa
     1141 tgggtatgaa gatcattgct ttgaaaaaaa cttttgaaca aatagattat gttgaagaat
     1201 tatatactcc tgataagatg gacgaaatct ttaaaaagag tgattatatt gttaatctgc
     1261 tgccctatat tcctgaaaca gataagatta ttaataagaa atattttgaa cttatgaaac
     1321 cgacagcttc ttttattagt gtggggcgtg gtaaaaccgt taatgaagag gatttgattg
     1381 aagcgctgag aacaaaaaag ataaaaggtt tggttagtga tgtattttat gaagaaccat
     1441 tgtctgaggg aagcccctta tgggagatgg aaaatgtttt tattactcct catgtggcag
     1501 gacagagcgt taagtatata gaaaaagcac tgggaattat cagacacaat ctcggagttt
     1561 atttgagcgg ggaaggggaa tacttaaata gaatagatta taagaagggt tattgaatac
     1621 agaattgttt gcactgatga ttatttaaaa atacgatatt acagtaatag tttgtataaa
     1681 aactgctatt accatagagg attgatatgg aaaaagtgta aggagtgaat aagatgaatg
     1741 ttagggaatt atcagaaaaa tatcagaatg aaattgttgc cttgaggaga gaatttcatc
     1801 aaaatccaga acttagctgg caagaatata atacagctgc aaggatagag aaagaattga
     1861 ataaaatagg gattaatgat actaaaagaa ttgctggcac gggggtagtc agctcaataa
     1921 aagggaaaaa aggtaataaa gtagttgcct tgagagcgga tattgatgca ttgcttgttc
     1981 aggaagaaaa tgaaacattt tatcggtcta aaaatgatgg aataatgcat gcctgtggac
     2041 atgatgccca tactgctatg cttttgggag ctgcaaaagt tttatgggaa cttagagaac
     2101 aatttgaagg cacagtaaag ttaatctttc aaccagcaga agaggttatt gagggagcta
     2161 aaaagatgtt agaagaagga gtattagaag gtgttgattc aattttggcc attcatgtct
     2221 ggtccgaact tctttctggt aaggtttctt tagaagaggg ccctcgtttt gcagctggtg
     2281 atcgattcaa gattgctatt aaagggaaag gaagccatgg ggcaatgcca catatgggta
     2341 tagatgctat tgtggctgct tcagccgtag tcatgaatct acagaccatt gttagccgag
     2401 agattgaccc tcaggaacca gcagtaataa g
//
LOCUS       DAOQWT010000228         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_48501_length_2430_cov_3.526316, whole genome shotgun sequence.
ACCESSION   DAOQWT010000228 DAOQWT010000000
VERSION     DAOQWT010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48501_length_2430_cov_3.526316"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..468
                     /locus_tag="PKJ95_05140"
     CDS             25..468
                     /locus_tag="PKJ95_05140"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNR65660.1"
                     /translation="MKGFAHNIKEDIEKKLNLPIFTVIKNWLDRYFVGEKPAIFELPL
                     APRGSEFQQNVWNILIEIPYGEVTTYGKIAQKIATLQGKNRMSAQAVGGAVGHNPISI
                     IITCHRVVGSDGSLTGYAGGIDTKLKLLEHEGVDVSNLSTPKRTP"
     gene            complement(536..1270)
                     /locus_tag="PKJ95_05145"
     CDS             complement(536..1270)
                     /locus_tag="PKJ95_05145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012985936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNR65661.1"
                     /translation="MANSISVAGIDIEVKKKNIKNMHLSVLPPDGRVRISAPLRTKDE
                     TIKLFAITKIGWIKKQITKFENQPRQTEWEYVSGESHYLWGRRYRMEINYYNSGRNKV
                     EIKGNRLILTVREASSKEQRERVMNKWYRKLLKEKTQELAGQWEKIIGVEAQSVQVRN
                     MLTRWGSCNVRKGSILVNLQLAKKPVECLEYVVVHELVHLLEKSHNSVFVGYMDEYLP
                     DWRIRKNILNDFIRDRYVKEYATETE"
     gene            complement(1335..1529)
                     /locus_tag="PKJ95_05150"
                     /pseudo
     CDS             complement(1335..1529)
                     /locus_tag="PKJ95_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001653610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(1552..2394)
                     /locus_tag="PKJ95_05155"
     CDS             complement(1552..2394)
                     /locus_tag="PKJ95_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNR65662.1"
                     /translation="MIGAIIGDIVGSRFEFNNYRSKNFELFTDECMVTDDTIMTIAVA
                     KAIMETEKLIDASSGRDRLENNYYSLLDKMAIKYMQEIGRRYPHCGYGGMFFRWVFSN
                     HPQPYNSFGNGAAMRISPVGFYAKSEEEVFRLSEAITGVTHNHVEGLKGAEATAIAVY
                     LASSGKSKDDIRNRIISNYYPLDFTIDGIRDTYQFNETCQDTVPQAIEAFLESDSFED
                     AIRTAISVGGDSDTLAAITGGIAEAYYGVPKDIKEKALSYLDDYLLSIYNDWLTFLKG
                     RTIF"
BASE COUNT          718 a          542 c          390 g          780 t
ORIGIN      
        1 gttaagagat tttcagccca atacataaaa ggctttgccc acaatataaa agaagatatt
       61 gaaaaaaaat taaaccttcc catttttaca gttatcaaaa attggctgga ccgatatttt
      121 gttggagaaa agccggctat ttttgaatta ccgctagcac ccagaggtag cgaatttcaa
      181 cagaatgttt ggaatatact tattgaaata ccctatggag aggttaccac ttacggaaag
      241 attgcccaaa aaattgctac attacagggc aagaacagga tgtctgccca ggcggtcgga
      301 ggggcagtag gtcataatcc catatcaatt attattacct gtcatagggt tgtgggttca
      361 gatggcagcc taaccggtta tgccggaggt atcgatacca aactcaaatt actagaacac
      421 gaaggcgtag acgtttcaaa cctatccaca ccaaaaagaa caccctagaa accatagata
      481 tcgattatag aaaattggaa aagtaaatgt attgtattgc ttttttataa ttatcttatt
      541 ctgtttctgt cgcatattct ttcacatatc tatctctgat aaaatcattt aaaatatttt
      601 ttctaattct ccaatcaggg agatattcat ccatataacc aacaaatact gaattatggc
      661 ttttttccag taagtgaacc agctcatgta ctaccacata ttccaggcat tctactggct
      721 tcttagccaa ttgtaggttg accaagatac tcccctttct tacattgcag gacccccacc
      781 tggtcagcat atttcttacc tgcacagatt gagcttctac cccaataatc ttttcccatt
      841 gacctgccaa ctcctgagtc ttctctttaa gcagctttct ataccatttg ttcataaccc
      901 tctctctctg ctctttgctg cttgcctccc ggacagtcag aatcaatcta tttcccttaa
      961 tttctacctt attccttcca gagttataat agtttatttc catcctgtac cgtcttcccc
     1021 aaaggtaatg actttcaccg ctaacatact cccactcagt ttgcctgggc tggttttcaa
     1081 atttggttat ctgtttttta atccagccaa ttttggtaat ggcaaatagt tttatggttt
     1141 catcttttgt cctcagtggt gccgaaattc tcaccctgcc atccggcggt aaaactgata
     1201 gatgcatatt ctttatattc ttcttcttta cctctatgtc aatccccgct actgaaatac
     1261 tattagccat aatactcttc ctgttctctc actatattaa atatcttatc aactgtatca
     1321 taataaataa cattaatcat gtcattgaag ctgccgcctt cgggaatctc tatctcagcg
     1381 tagcggtcac cctgaaagcg gtctgtgaca tattttacaa aaaggagtgt taaaacataa
     1441 tctttgtata gggaagcatc cattcttccc cttaactcat tacagctatt ccacaatgaa
     1501 ctgtatattt cgctcttttt taccggcatt tatattaccc cttaaaaaaa gttaaaaaat
     1561 tgttctacct tttaaaaaag tcagccaatc attatagatg gagaggagat aatcatctaa
     1621 ataggagagg gctttttctt tgatgtcctt aggaactccg tagtaagctt ctgcgattcc
     1681 accggtaata gcagccaggg tatcactgtc gccaccaacc gaaatggcag ttctgatagc
     1741 atcttcaaaa gagtcagatt ccaaaaatgc ctcaattgcc tgtggtacag tatcctgaca
     1801 ggtttcattg aattgatagg tatccctgat gccatcgatg gtgaaatcga gggggtaata
     1861 attactgata atcctatttc taatatcatc ttttgatttg ccagaactgg ccaaatagac
     1921 agctattgcg gtagcctctg cacccttcaa gccttctaca tgattgtgag taactccggt
     1981 tatggcctca gaaagcctga aaacttcttc ttcacttttg gcataaaaac caaccggact
     2041 tattctcata gcggcaccat ttccaaagct gttgtatggt tgaggatggt tactgaatac
     2101 ccatctaaag aacattccac cataaccaca atggggatat ctacgtccaa tttcctgcat
     2161 atatttgata gccatcttat cgagaagtga ataataatta ttctctaatc tatccctgcc
     2221 agatgaggca tcgattaatt tttctgtttc cataattgcc ttggcaacag caatggtcat
     2281 aatggtgtca tctgttacca tacattcatc ggtaaagagc tcaaaatttt tacttctata
     2341 attgttgaat tcaaatctgg aaccaacaat atctccaata atcgccccaa tcattagaaa
     2401 ccctccttta aatagtatta tctaaaataa
//
LOCUS       DAOQWT010000229         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_48631_length_2426_cov_3.048925, whole genome shotgun sequence.
ACCESSION   DAOQWT010000229 DAOQWT010000000
VERSION     DAOQWT010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48631_length_2426_cov_3.048925"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKJ95_05160"
     CDS             complement(<1..186)
                     /locus_tag="PKJ95_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65663.1"
                     /translation="MRNSNDRPDIRIRAHTKRLLDKVIEKTGKKQIALASEILEEGLK
                     KYLSEDEQVTSHKLYSDL"
     gene            complement(534..851)
                     /locus_tag="PKJ95_05165"
     CDS             complement(534..851)
                     /locus_tag="PKJ95_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65664.1"
                     /translation="MLKVECPECGRVNSAPIEGSFPGCDGIDDDLKTDFNCGCGNLFN
                     VTYILTEKRSYNRCEICREYEFNCDLIKAKEAGVYFCKDCIDKSYSADTTQGTTAGEL
                     SNV"
     gene            complement(901..1197)
                     /locus_tag="PKJ95_05170"
     CDS             complement(901..1197)
                     /locus_tag="PKJ95_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65665.1"
                     /translation="MEPGIYLKCEAGERYFNSVQDLRRAIGAENLSHPNLWSLNMWEL
                     ILFYTEVIMKERKAVITFVSRLKYIVNFYIDANEEKIHPMIAWRAERHGQLKLF"
     gene            complement(1272..1640)
                     /locus_tag="PKJ95_05175"
     CDS             complement(1272..1640)
                     /locus_tag="PKJ95_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65666.1"
                     /translation="MEFNDMELDDEFFDKLLIGIKIEKNQKEIEKNQKEIVEIRKVIE
                     REEKNIVDAEEELTKIWVRKKQIEVFSILHSHSVYTVVNLYSSHMFIGGTATPIIKLS
                     IVLYEILGKEIAENLKTLVE"
     gene            complement(1730..2266)
                     /locus_tag="PKJ95_05180"
     CDS             complement(1730..2266)
                     /locus_tag="PKJ95_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65667.1"
                     /translation="MTGCSYKTKDFYGFLLYIDEINKIKRSGLDKMTMKEQEEKLSEN
                     WKKFQEWQEEQENTKQPEQEQKNKAKCIFDTWPENLRIFFKDRGTQDFFSDFFIYKLD
                     DIKVENEKIPEPSEITFLCRNYAYSGFDAGLLIGYAFSRYLDKPLQKFRDEAERISKH
                     ELKSIKELTEQWNKDCRI"
BASE COUNT          653 a          547 c          298 g          928 t
ORIGIN      
        1 caggtcacta tacagtttat gagatgtaac ttgttcatct tctgacaaat atttttttaa
       61 tccttcttcc aggatttcac tggcaagagc aatttgcttt ttccctgttt tttctataac
      121 tttatcaagt aatctcttcg tgtgtgctct tattctaata tccggcctgt catttgaatt
      181 tctcatacta tatccctttt taattttcta tggtaaaatc cattcatact tataattttt
      241 cttattttct atagttgagc cggaattgac tgttccggct ccctctgttt atatccgcct
      301 atcttctaac aggtcttata ttctaacagc ttccaagata tccctgtcag atagatcttc
      361 ttttcatata agcctttttg aaattatact caatacacct tgtacttgct atggttaatg
      421 gtgttaatat gaaatatgta atataaaata gttccataaa ttccctcctt cctcttctgg
      481 atggtccgtg ccggaccttc tagagtctta aaaagccttg aaaataaagg attttacaca
      541 ttcgacagct cccctgctgt agtgccctgg gtagtgtcgg cagaatagct tttatcaatg
      601 cagtctttgc aaaaataaac gcctgcttcc ttcgccttaa tcaggtcaca attaaattca
      661 tactccctgc aaatctcaca tctattatag cttctctttt cggtcaggat ataagttaca
      721 ttaaagagat taccacagcc acaattaaaa tccgtcttta aatcatcgtc tataccatca
      781 cagccgggaa aagaaccttc aataggtgca gaattcaccc ttccacattc aggacattcc
      841 acttttaaca taggtcattc ctcctttttt catcccttcg ccctgggtag gagaaggaaa
      901 ttaaaataat tttaactgtc cgtgcctttc cgctctccag gcaatcatgg gatgtatttt
      961 ttcttcattg gcatctatat aaaaattcac tatatacttc aatctcgata caaaagttat
     1021 caccgctttc ctttccttca ttatcacttc tgtataaaaa aggataagct cccacatgtt
     1081 taaactccac aggtttggat gtgataaatt ttccgctcct attgcccttc ttaaatcctg
     1141 aacactatta aaatatcttt ctccagcttc acacttcaga tatattcccg gttccataat
     1201 ttcctcccct tcgccctggg cagtagaaga gagaaaagaa gatctccttc tctctcttcc
     1261 ggctgttttt tttattcaac aagagtcttt aaattttctg ctatttcttt tcccaggatt
     1321 tcatataata caatagacag ttttattatt ggcgttgctg tccctcctat aaacatatga
     1381 gaactatata aatttacaac agtatatact gaatgagaat gtaagatact aaaaacttct
     1441 atttgcttct ttcttaccca tattttagtt aattcttctt ctgcatcaac tatatttttt
     1501 tcctctcttt caataacttt acgtatttca actatttctt tctgattttt ctctatttct
     1561 ttctgatttt tctctatctt tataccaatt aataatttat caaaaaattc atcatctaat
     1621 tccatatcat tgaattccat aattaatctc tccttctatt gcaaattttc agggagcagg
     1681 gcagaaaaga agatctcctt ctctctcttc cggctcccct ggctatctat tatatacggc
     1741 aatctttatt ccattgctct gttaattctt taatactctt taattcatgt tttgatattc
     1801 tttcggcttc atctctaaat ttctgtaatg gtttatcaag gtaacgagaa aaagcataac
     1861 cgattaataa gcccgcgtca aaaccactat aagcataatt cctgcataaa aaagttattt
     1921 ctgatggttc aggtattttt tcattttcca cttttatatc atccagttta taaataaaaa
     1981 aatcactaaa aaaatcctgg gttcctctgt ctttaaaaaa tattctcaga ttttccggcc
     2041 atgtatcgaa gatacatttt gctttatttt tctgctcctg ctctggttgc tttgtattct
     2101 cctgctcctc ctgccactcc tgaaatttct tccaattctc tgataacttt tcttcctgct
     2161 ctttcattgt cattttatca agtccgctcc ttttaatttt attaatttca tctatgtata
     2221 ataagaatcc ataaaaatct tttgttttat agctacaacc tgtcatttat tataatctct
     2281 ccttctgata atcttgaaaa ataggggtta tttatcagaa cacatcaccc actttcccgg
     2341 cctccgtgcc agtcctgcag gtgtttttta cccccccccg aaaattacat cagccctttc
     2401 cgttatgtta agtctttttg cctcta
//
LOCUS       DAOQWT010000230         2424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_48704_length_2424_cov_2.295483, whole genome shotgun sequence.
ACCESSION   DAOQWT010000230 DAOQWT010000000
VERSION     DAOQWT010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48704_length_2424_cov_2.295483"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..530
                     /locus_tag="PKJ95_05185"
     CDS             255..530
                     /locus_tag="PKJ95_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNR65668.1"
                     /translation="MNKSELIDKVSYDRGVTKAHAKAAVECILENIAEALVRKESVRL
                     VGFGTFGVRERAARTARNPRTGKNIPLPKATVPFFKPGKELKERVNK"
     gene            complement(606..1130)
                     /locus_tag="PKJ95_05190"
     CDS             complement(606..1130)
                     /locus_tag="PKJ95_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNR65669.1"
                     /translation="MQENETEAKKLKKEVKKKDKDMSLVEKVVKEEELPLQVKENSGK
                     KEKILKNTLGEINISPEVLAAIVSRIVNGISGVAGLFAQSKSGIGTLLGVKEIEEGIK
                     VDLIEGKSLSTYISVIVDYGSVIIDLAKKIQSVVKSEVEEKTGLFVKSIDVNVMGIQM
                     PGKDKESKVKTGNN"
     gene            complement(1194..1457)
                     /locus_tag="PKJ95_05195"
     CDS             complement(1194..1457)
                     /locus_tag="PKJ95_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HNR65670.1"
                     /translation="MELLKVSSKSNPKAVAGALAGVIREKGKVELQAIGAGAVNQAIK
                     AIAIARGYTATSGVDLVCIPAFVDVEINGDERTAIKFFVTPIG"
     gene            complement(1720..>2424)
                     /locus_tag="PKJ95_05200"
     CDS             complement(1720..>2424)
                     /locus_tag="PKJ95_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR65671.1"
                     /translation="SRGCTFRCTYCASHILNPKLEFRNPEQVAKEIAYWKELKGIDKF
                     VFYDDALLVNSENHIIPFLNEIKKKNLNINFYTPNALHVRYITQPVAKLMLECGFEKI
                     WLGFETADSELQRKTGNKVNNASFTKAVKNLLKEGFPPEKIRAYLLIGLPDESLQSII
                     ESIKVVMDNGIKPYLAKYSPIPGTKMWKEIVREYKWKEPVDPLWHNDALMPYCSPYIN
                     VEQYQQIKMIIQNFKL"
BASE COUNT          768 a          439 c          317 g          900 t
ORIGIN      
        1 cctattgttt tatttactca ttaagttcaa attatacact aaaattttag aacaatttga
       61 agattgaaca aattattaac ccatttataa aaaaattggt ttttgtgatt aaaatacaaa
      121 aataattgac aaaagccttt atttttaata ttcttaggat aatatgattc atatggtaaa
      181 ctatttaaaa tgtacctgac actgaattag aaagaatttt tatttatcgt atagtagaaa
      241 ggaggtgtaa tattttgaat aaaagtgaat taattgataa agtatcatat gacagaggag
      301 taaccaaagc tcatgccaaa gcagctgtgg aatgcatact tgaaaacatt gctgaggcat
      361 tagtcagaaa agagtctgta cgtttagttg gttttggaac ttttggtgtt agagaaagag
      421 cagccagaac tgcacgcaat ccaagaacag gtaaaaatat tcctttaccg aaagcaactg
      481 ttccattttt taaacctggg aaagaattaa aagaaagagt taataaataa agcaataata
      541 ttttacttca aataaaacag gtctgtcttt ttaataattt acagacctgt tttttatgta
      601 tatttttaat tattacctgt tttgacttta ctttccttat ctttaccggg catctgaatt
      661 cccataacat ttacatcaat ggattttaca aaaagtcctg ttttttcctc tacttcgctt
      721 tttaccacag actgaatttt tttggctaaa tctattatta ctgagccata gtcaacaatc
      781 acggaaatat aggttgatag tgatttaccc tctatcaaat caaccttgat tccttcttct
      841 atctctttta caccaagtag agttccaatt ccactcttag attgggcaaa taaaccggct
      901 acaccggaaa taccgttcac aattctacta acaatagcag ctaatacttc aggagaaatg
      961 ttaatctctc ctaaggtatt tttaagaatt ttttcttttt ttcctgaatt ctcttttacc
     1021 tgtagaggca gttcctcttc ttttacaact ttttcaacca atgacatatc tttatctttt
     1081 ttcttgactt cttttttcag cttttttgct tctgtttcat tttcttgcat agtaattact
     1141 ccttaaataa tgatgtaaaa tgactcccca tattcagtgt taaaaaaatc attttatcca
     1201 atgggagtaa cgaaaaattt aatcgcggtt ctctcatcgc cgttaatttc aacatctacg
     1261 aaagcaggaa tgcaaactaa atcgacacca cttgttgctg tatatcctct cgctattgca
     1321 atagccttaa ttgcctgatt cacagcccct gcacctattg cttgtaactc aacctttcct
     1381 ttttctctaa tgactccagc caatgcacca gcaactgctt ttggatttga ttttgaagaa
     1441 acttttaaca gttccatctt aagcctccta tacctatatc tatatatacc aacatctatt
     1501 cttccatcat tgtaaatatg gttgctaatt cgccttttat tacttttatt ttgtatatat
     1561 acttcttggt gcaaaaaatc ttgacctctc acacacctcc ttttaattac tattgaagta
     1621 caaagccttg tcttccaata aaaatttctt cttcttttat tattataaca aatttaattg
     1681 ataaatgaca attagattac tctttgagtt ttttgttaat cataatttaa aattctgtat
     1741 tatcatcttt atctgttgat attgctctac atttatatat ggtgaacaat aaggcatcaa
     1801 agcatcatta tgccacaagg gatcaacggg ttctttccat ttatattctc tcacaatttc
     1861 tttccacatt tttgttcctg gaataggtga atattttgcc agatatggtt taataccatt
     1921 atccattaca actttaatgg actcaattat actttgcaaa ctctcatctg gtaggccaat
     1981 taaaagataa gcccgaatct tttccggggg aaacccttcc tttaataaat tcttaactgc
     2041 ctttgtgaaa gaagcgttat ttactttatt tcctgttttc ctctgcaatt ctgaatcagc
     2101 cgtttcaaat cctaaccata tcttttcgaa cccgcattca agcattaatt tagccactgg
     2161 ttgagtaata tatctgacat gtaaagcatt aggtgtgtag aaattaatat ttaaattctt
     2221 ttttttaatt tcatttaaga aaggaatgat atgattttct gaattaacca ataaggcatc
     2281 atcataaaaa acaaatttat caattccttt taattctttc cagtaagcta tctctttggc
     2341 aacttgttct ggattacgaa attctaactt aggattaagg atgtgagaag cacaataagt
     2401 acatctaaaa gtgcaacctc ttga
//
LOCUS       DAOQWT010000231         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_48784_length_2421_cov_3.441251, whole genome shotgun sequence.
ACCESSION   DAOQWT010000231 DAOQWT010000000
VERSION     DAOQWT010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48784_length_2421_cov_3.441251"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /gene="nagA"
                     /locus_tag="PKJ95_05205"
     CDS             <1..853
                     /gene="nagA"
                     /locus_tag="PKJ95_05205"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNR65672.1"
                     /translation="IKSLQSIREARLKGTGTAEILGVHLEGPYINPEKKRAQNKKDII
                     QLSKKEFLAFNQASGNLIRLVTLAPEMPGALDFIPWLCEQGIITSAGHTNATYEEMQD
                     ALQSGLNHVTHLFNAMRGLHHREPGIVGAALSDSRITAEVIADGIHIHPVILKIIHQM
                     KGSNKIVLITDAMRASGLPEGTYDLGGQEVIVAKEQARLQDGTLAGSVLTMNKAVQNI
                     VAKAGIPLGEAVQMASYNPAKCLGINNKKGSLETGKDADIVMLNKNFEVDLTMVSGKV
                     IFRREEL"
     gene            850..1575
                     /gene="nagB"
                     /locus_tag="PKJ95_05210"
     CDS             850..1575
                     /gene="nagB"
                     /locus_tag="PKJ95_05210"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNR65673.1"
                     /translation="MNVVIVKNYQELSVRAAQLVADQITRKKNIVLGLATGQTPVGMY
                     QGLIKKYKKGEIDFSQVVTFNLDEYYGLSPEHPQSYHYFMWDTFFQHININKEKIYLL
                     NGITDNIDEECQRFEDLIKKRGGIDLQILGIGDNGHLGFNEPAIGLKSKTHLVNLSKA
                     TIHANVKYFNDIKEVPRQALTMGIGTIMKAEKIILLASGRKKSPVIVKTIKGPVSTEV
                     PATVLQLHNEVTIILDQDVASKL"
     gene            1941..>2421
                     /gene="rhuM"
                     /locus_tag="PKJ95_05215"
     CDS             1941..>2421
                     /gene="rhuM"
                     /locus_tag="PKJ95_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HNR65674.1"
                     /translation="MSNENEIIIYTTQDGKETFEVNLKEETVWLSQRQMASLFEKDSD
                     TIGLHIKNIFSTNELDEKSTTEESSVVQLEGKREVRRKIKFYNLDVIISVGYRVNSKR
                     GTQFRIWATNILKNHLIQGYTINQNRLQESQAKIKELQKTSRMIPIARHNYMRLLRNF
                     "
BASE COUNT          853 a          465 c          477 g          626 t
ORIGIN      
        1 cataaaaagc ctccagagca tcagggaagc cagattaaag ggtaccggaa cagcagagat
       61 tttaggagta cacctggaag gaccctatat caacccggag aaaaaaagag cccaaaacaa
      121 aaaagatatc atccagctct ccaaaaagga atttctggcc ttcaatcaag cctccggcaa
      181 tttaattcgc ctggtgaccc tggcaccgga aatgcccgga gcactggatt tcattccctg
      241 gctgtgtgaa caaggtatca ttacctcagc agggcatact aatgctacct atgaagagat
      301 gcaggatgcc ctacaatccg gtctaaacca tgttacccat ctgtttaatg ccatgagagg
      361 attacaccat cgagagccgg gaatagtggg tgccgcttta tctgattcca gaataacagc
      421 agaggtcatt gctgatggaa tacatatcca tccggtcata ttgaaaataa tacaccagat
      481 gaaggggagt aataaaatag ttctaattac tgatgctatg agggcaagcg gcttgccgga
      541 aggcacctat gatctgggag gacaggaagt gatagtggct aaagagcagg ccaggctcca
      601 ggatggtact ttagccggta gcgttctaac tatgaataag gctgtacaga atattgtagc
      661 taaagcaggc attccattag gggaggccgt tcaaatggcc agttataatc cagcaaagtg
      721 tctgggtata aataataaaa aaggaagtct ggaaacaggc aaagatgcag atatagtcat
      781 gctaaataaa aactttgaag tagacttaac tatggtttca ggaaaagtta tcttcaggag
      841 agaagagcta tgaatgtcgt cattgtaaaa aactaccagg aattaagcgt tcgggcagct
      901 cagctggttg ccgatcaaat caccaggaag aaaaatatag tcctgggttt ggctaccggg
      961 cagacaccgg tgggcatgta tcagggattg attaaaaaat ataaaaaagg agaaatagac
     1021 ttttcccagg tggtaacctt taacctggat gaatactatg gcttatcccc agagcacccg
     1081 caaagttacc actattttat gtgggatacc ttctttcagc atataaacat caacaaagaa
     1141 aagatatatt tattaaacgg aataacagat aatatcgatg aggaatgcca gagatttgaa
     1201 gatctcatta aaaaaagagg tggcatagac ttacagatat tgggcattgg tgacaatggg
     1261 catcttggtt ttaatgagcc ggccatcggt ctaaaaagca agactcacct ggtaaattta
     1321 tcaaaagcta ctatccacgc caatgtcaaa tattttaacg atatcaagga agtaccccgg
     1381 caagctctca ccatgggtat cggaacaatc atgaaggctg aaaaaattat tctcttagct
     1441 agtggccgaa agaaatctcc agtaatagtc aagactatca agggaccagt cagcactgag
     1501 gtaccagcaa ctgttcttca gcttcataat gaggtaacta ttattctgga tcaagatgtt
     1561 gccagcaaac tttaatctaa cctgctaagg ataaaacaac ctcttgctct tctcctaaga
     1621 ctataaatct aaatacaaaa atgcccgagc ttgtgattct gatttaataa ttgatttttt
     1681 tcctaaaaca aaataatttt tctaaaaata ttttattgca ctttatctac aaagcaatct
     1741 catccaatta tacccaaaaa aatatttatt tgctttttgc ttttcactca tcactgatca
     1801 ccaattgctg tatttaactt gaaccttgta ccttgaatct tgcaactttc ttttaaccgt
     1861 caaataaacc aggaaatcta aataaacttc tggtaaaata agtatgattg ctaaattatt
     1921 gtaaaaggat aaaaagcaca atgtctaacg aaaatgaaat tattatctat accacacaag
     1981 atgggaaaga aacctttgaa gtcaatttaa aggaagaaac tgtctggctt tcacaaaggc
     2041 agatggccag tctgtttgaa aaagacagtg acaccattgg actgcatatt aaaaatatct
     2101 tttctacaaa tgagctggat gaaaaatcaa ctaccgagga atcctcggta gttcaattgg
     2161 aaggtaaaag ggaagtaaga agaaaaatta agttttataa ccttgatgta attatttctg
     2221 ttggctaccg tgttaactcc aaacgaggaa cccaatttcg tatctgggca accaatatcc
     2281 tgaaaaatca tctcattcag ggatacacca tcaatcaaaa tagattacag gaaagccagg
     2341 ccaaaattaa agaattacaa aaaacctcca gaatgattcc aatcgcccga cataattata
     2401 tgagactact tcgtaacttc a
//
LOCUS       DAOQWT010000232         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_48806_length_2420_cov_3.624947, whole genome shotgun sequence.
ACCESSION   DAOQWT010000232 DAOQWT010000000
VERSION     DAOQWT010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48806_length_2420_cov_3.624947"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1884)
                     /locus_tag="PKJ95_05220"
     CDS             complement(163..1884)
                     /locus_tag="PKJ95_05220"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNR65675.1"
                     /translation="MKKRPDSFSWKNSSTWKFFLNTLISFLVIVIFVTVAYYYFLNEN
                     EKTNLNNNISHIIEDHTPFLVSSLWITDYNAIHQDLGVIARIKYIDRVEVEDEDGTIF
                     YTGEEAKPFYKIISHDLIYNYRDEQKKIGVLKIFTNERNIRAEVIKNTAFILLIEIVL
                     SLILAAIIAAIFYSFIGRHLLQFAQFLEDDDPQKESCSFNLERASSRNDELQILVDHF
                     NKLREQLGCYITEMRHFSYVDKLTDLYNHRFLMEELKRLDTERNLPLSVIVLDLDHLK
                     LTNDTFGHAAGNKLLQRAAAVLKGEFRADEIIARVGGDEFVVLLPKIDKKQAGIMVKR
                     VMSAFEREEVKHIPLSASFGWDTKENKEQEIASTLKKAEDHMYSQKLINRNKKRKSSI
                     ESILQALFKKEPREEIHSERVSKISENIALAMGMNPSEIYELKMAGKYHDIGKIALSP
                     DILSKAGILTTSERLELKRHSELGYSILNTIEELSSIANIVLYHHERWDGKGYPGGLK
                     GEDIPIQSRIIAVANAYGEMIEGYRNLPGALSEEEAIKELEKGAGSQFDPAIVALFTN
                     KAVDLDV"
     gene            complement(1905..>2420)
                     /locus_tag="PKJ95_05225"
     CDS             complement(1905..>2420)
                     /locus_tag="PKJ95_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNR65676.1"
                     /translation="VEINRIEGMEALYPNLIRVPEPNMVMNFVAFAKQDFKIDGWESI
                     KDLYIGIVKGWKILEDNTADFPNVTIVPTETELFTMLEKGRIDIALYCSLDGYEQLIL
                     LGLKDIHHLEPPLASRNMYLYLNKKHQELVEPVAQALRDMKEDGTYQKIVQETTGHLK
                     LAPKKIGQEQE"
BASE COUNT          688 a          514 c          395 g          823 t
ORIGIN      
        1 agaattttta aatttttttt attatttccc atatgaaaat aaagaagaag aataaaaaaa
       61 caatattgga gtacttctct ttctataata ataaaatgta ttttatgaaa taatatcaac
      121 ttgtgccgcc aattcagttc caccagattg aacctaagtc tattacacat ctaaatcaac
      181 tgccttattg gtaaacagag ctactatagc cgggtcaaat tggcttcccg cccccttttc
      241 taactcttta attgcctctt cctcgctcaa agctccggga agattacgat acccctctat
      301 catctctcca taggcattag ccactgcaat aatcctggat tgtatgggaa tatcctctcc
      361 ctttaaaccg ccaggatacc ccttgccatc ccatctctcg tgatgataaa ggacaatatt
      421 tgctatcgaa ctgagctctt caatagtgtt cagaatagag tatcccaatt ctgaatgtct
      481 cttcaattct aatctttccg aggtggtaag tatgccagcc ttactaagaa tatcagggct
      541 gagggctatt ttcccaatat catggtattt gccagccatt ttcaactcat atatttctga
      601 gggattcatt cccatggcca gggcaatatt ttcactaatt ttacttaccc tttcagaatg
      661 gatttcctct cggggttctt ttttaaataa tgcctgcaat atgctttcaa tagagctttt
      721 tctcttttta tttcggttaa ttaatttttg actgtacata tggtcttcag ctttttttaa
      781 ggtgctggcg atttcctgtt ctttattctc tttggtatcc cagccgaaag aggcagataa
      841 aggtatatgt tttacttctt ctctttcaaa agctgacata actcttttta ccattatgcc
      901 cgcttgtttt ttgtcaattt tgggtaacaa cactacaaat tcatccccgc ctaccctggc
      961 tataatctca tcagcacgaa actcaccctt tagcacagcg gccgctctct gtaaaagttt
     1021 attacctgca gcatgaccga aggtgtcatt ggttagtttt aaatggtcta aatctaatac
     1081 tattacactt aaagggaggt ttctttcggt atccaaacgc ttcaattctt ccattaagaa
     1141 acgatggtta tagaggtctg tcaatttatc tacataacta aaatgtcgca tttcagtaat
     1201 gtagcatccg agctgttcgc gtaatttatt aaaatggtct accagtatct gcagctcatc
     1261 attacgggaa gaggcacgtt ccaggttaaa agaacagctc tccttttgcg ggtcgtcatc
     1321 ttcaagaaat tgtgcaaatt gtagtaaatg ccgaccaatg aaggaataaa agattgcggc
     1381 aataatggca gccaggatta aagacaatac aatttctata agaagtatga aggcagtatt
     1441 ctttattact tctgctctta tatttctctc attggtgaag attttcagaa caccaatttt
     1501 tttctgctca tccctataat tataaatcag gtcatgggag atgattttat agaatggctt
     1561 tgcctcttct ccggtataaa aaatagtacc atcttcatct tccacttcta ctctatctat
     1621 atattttatc ctggcaataa cccctaaatc ttggtgtatg gcattataat cggtaatcca
     1681 aagagaagag accagaaaag gagtatggtc ctctataata tgggaaatat tgttgtttaa
     1741 attggttttt tcattctcat ttagaaaata ataataggca acagttacaa aaataacaat
     1801 gaccaggaaa ctaatcagtg tattaagaaa aaatttccag gtactggagt ttttccagct
     1861 gaaactatca ggtcttttct tcatttaaaa aatacctctt gctcttactc ctgctcctgc
     1921 ccaatctttt ttggtgccag ctttagatgc ccggtggttt cctggactat tttttgatag
     1981 gtgccgtcct ctttcatatc ccgtaaggcc tgagcaaccg gttctaccag ttcctgatgt
     2041 ttcttgttca aatagaggta catattcctg ctggccaagg gtggttccag atgatggata
     2101 tcttttaaac cgaggagtat aagctgttca tagccatcca aactgcaata cagggcaata
     2161 tctatcctcc ctttctccag catggtaaac aattcggttt cagtgggcac tattgtcaca
     2221 ttaggaaaat ctgcagtatt atcttccaga attttccaac ctttaactat cccaatatat
     2281 aaatccttta tgctttccca accatctatc ttaaaatcct gtttggcaaa agctacaaag
     2341 ttcattacca tatttggttc cggtacacga atcaggttgg gataaagtgc ttccatacct
     2401 tctattctat ttatctccac
//
LOCUS       DAOQWT010000233         2419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_48850_length_2419_cov_3.094332, whole genome shotgun sequence.
ACCESSION   DAOQWT010000233 DAOQWT010000000
VERSION     DAOQWT010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48850_length_2419_cov_3.094332"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2419)
                     /locus_tag="PKJ95_05230"
     CDS             complement(<1..>2419)
                     /locus_tag="PKJ95_05230"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNR65677.1"
                     /translation="ITEALHLYQDLLSFTNLPDDKHIFIYPPHEVLPYEDLSPDSQIV
                     KQRIKVLSILAHLQCRQPNKCNSHLPLIIISTYRAILPKIMPLQQFHQQHLKLKLGGQ
                     LKIEYLLNYLLEQGYQYSNLIETEGQFSQRGGIVDLFPLTEEIPLRIEFDGDRIASLR
                     YFDPESQRSIQRVEGILLLPKRELSLKSEAKDGETNTFFDYLPEYCDIFISQFSDFEN
                     NVRDFERESENSFLNKKDTNKGILPPSYYYLNWNQVNKLIYQKQRLVKITSWLEQEYS
                     PSKEIKGMEYQPFSIESKPTENYFGNLELFFKNIKKSRREKQNILILTRNKGRALRMA
                     EIFEDRGITDYQMMALPEAEVYPGKICLSYGLVNYGFSIPSLNLTVVTEQEIFGKQRD
                     RGYRSKRFQGKPFYQLDELRTGDYVVHINHGIGQFAGIKSRNTEGIRKDYILIQYASD
                     DELYVPVEQLSLVHKYIGVGGSTPKINRLTDGSWKTTKKRVKESVQKTARELYELYRK
                     RKSVQGFPFSPDTVWQQELEMAFPFEETPDQEKAFQDVKKDMELAQPMERLICGDVGY
                     GKTEIAIRAAFKAVMDGKQVAVLAPTTILAQQHWENFSERMKAFPIRVEMLSRFKSKK
                     EQQEIITDLKKGNIDIIIGTHRLVQQDIFFKDLGLLIVDEEQRFGVNHKERIKKLREQ
                     VDSLTLTATPIPRTMYLSLTGIREMSIINTPPELRLPIITFFKPKTDEIIREAIKREL
                     SRGGQVYYVYNRVQDIDSVAEKLNLLIPEAKAVIAHGQMPEEQLENIMIDFLNKKYNV
                     LICTTIIE"
BASE COUNT          699 a          491 c          413 g          816 t
ORIGIN      
        1 ttcgataatg gtagtacaaa ttaaaacatt atactttttg ttcagaaaat caatcattat
       61 attttccagc tgctcctcag gcatttgccc atgcgcaata acagcttttg cttcaggaat
      121 taaaaggttt agcttttctg caacactatc aatatcctga accctattat aaacataata
      181 gacctgaccg cctctgctca actctcgttt aattgcctcc ctgataattt catctgtttt
      241 cggcttaaaa aaagtaatta tgggcaagcg tagttcgggc ggagtattta taatactcat
      301 ctctctgatg ccggttaatg ataaatacat tgttctggga attggagtgg cagtcagagt
      361 taaagagtca acctgttctc ttaatttttt aatccgttct ttatgattaa ccccaaaccg
      421 ctgttcctcg tcaacaatta ataagccaag atctttaaag aatatgtcct gctgaacaag
      481 ccgatgagtg ccaataataa tatcaatatt tccctttttt aaatcagtaa tgatttcctg
      541 ctgttccttt ttagatttaa atctggaaag catttcaact ctgatcggaa aggccttcat
      601 cctttcagaa aaattttccc agtgttgttg tgccagtata gtggtaggtg cgagcaccgc
      661 tacctgttta ccatccatta ccgctttaaa ggctgccctg atggcaatct cagttttacc
      721 ataacctaca tccccgcaga ttaaccgttc cattggctga gctaattcca tatccttttt
      781 cacatcctga aatgcttttt cttgatcagg tgtctcttca aaagggaaag ccatctccaa
      841 ttcttgctgc catactgtat ctggagaaaa agggaagccc tgaacactct tcctctttct
      901 atataattca tataattcac gggcagtctt ttgaacagat tcctttaccc tttttttagt
      961 tgtcttccaa ctaccgtcag tcagacgatt tattttggga gtactccctc ctacgccaat
     1021 atacttatgg accaatgaga gttgttctac aggcacatat agttcatcat ctgaagcata
     1081 ctgaattaaa atataatctt ttctgattcc ttcggtattg cgtgatttaa ttccagcaaa
     1141 ttgacctata ccatggttaa tatgaactac ataatcacct gttctcaact catccaattg
     1201 ataaaaaggc ttgccctgaa acctttttga gcggtaacct ctatctctct gtttaccaaa
     1261 gatttcctgt tctgtaacaa cagttaggtt taaggagggg atagaaaacc catagttgac
     1321 caatccatag ctcaggcata ttttaccggg atatacttca gcctccggca aagccatcat
     1381 ctggtaatcg gtaatgcccc tatcttcaaa tatctctgcc atccgtaaag ctcgtccttt
     1441 gttacgagtt aaaatcagaa tattctgctt ttctcgcctg ctttttttta tatttttaaa
     1501 gaagagttcc agattaccaa aatagttctc tgtaggttta ctttctatcg agaaaggctg
     1561 gtattccata cctttaattt cttttgaggg agaatattct tgctctagcc aggatgttat
     1621 ctttactaaa cgctgttttt gataaatcaa tttgtttacc tgattccagt tcagatagta
     1681 gtaagatggg ggcaatatgc ctttattggt atcttttttg ttaagaaaac tattctcact
     1741 ttccctttca aaatcccgaa cattattttc aaaatcagaa aattggctta taaaaatatc
     1801 acaatattcg ggtaaataat caaaaaaagt attggtttct ccatcctttg cctctgattt
     1861 cagactcagt tctctcttgg gaagtaaaag aattccctca actctttgaa tagatctttg
     1921 cgattcaggg tcaaaataac gcagagatgc aatccggtca ccatcaaact ctattcgtaa
     1981 tggaatttcc tcagtaagag gaaatagatc tacgattccg ccccgttgac tgaattgccc
     2041 ttccgtttca atcagatttg agtattgata gccttgttcg agaagataat ttaaaaggta
     2101 ttctattttt aattggcctc ccagctttaa tttaagatgc tgttgatgaa attgctggag
     2161 gggcataatt ttagggagaa ttgcccgata agtgctgata ataatcaaag gaagatgaga
     2221 gttacattta ttaggctggc gacattgaag atgtgccaat atagataata cttttattct
     2281 ctgcttcaca atttgggaat caggggataa gtcttcataa ggtaaaacct catgtggggg
     2341 atagataaag atatgcttat catccggtaa gttggtaaaa cttaacaaat cctggtatag
     2401 atgaagtgcc tctgtaata
//
LOCUS       DAOQWT010000234         2414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_49017_length_2414_cov_2.533277, whole genome shotgun sequence.
ACCESSION   DAOQWT010000234 DAOQWT010000000
VERSION     DAOQWT010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49017_length_2414_cov_2.533277"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..217
                     /locus_tag="PKJ95_05235"
                     /pseudo
     CDS             97..217
                     /locus_tag="PKJ95_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            260..511
                     /locus_tag="PKJ95_05240"
     CDS             260..511
                     /locus_tag="PKJ95_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65678.1"
                     /translation="MQAEMADGAISGNVDGAVIIAEKGGMQNPYGGTVVVVKSGWTAV
                     AGAVCITPCGTDDGTCYKFQGYDNSATPKLFGDELTAQR"
     gene            1033..>2414
                     /locus_tag="PKJ95_05245"
     CDS             1033..>2414
                     /locus_tag="PKJ95_05245"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNR65679.1"
                     /translation="MSSSLVALYTLLQYYGFDPYYGYLSQLTSTIGQKNWLSNYLALI
                     FPITFSFFLLETNRRYKIILFLLLTILYTALIIGQSRGIWISIITTLLFSLVFIYRYG
                     LWEVFRNNKKWLIALLTIFLVVTVIYSTENPLNKSSLTVIERAASTFDENDPSINTRL
                     LILETTMEMIKNKPILGLGIGAFSYHYLDYQAAYLVKHPEYIKFSGKAAEAHNEYLHL
                     AAEIGIAGLFSFLTIIYIFYYLIYQYLEKEKNKNYKIIIFGLLAGVTCFLIHCLFTFP
                     FHVPVLGTTFFILLGLTIAYINLTEKGKGNDSAKNIVLIHFKFNALYRTIAIVILIVL
                     AVTGLWKIALKPYLAELYYFKGARHFAVHNNEVALKYFKKAALYDSNNGRVMHALGSA
                     YYQLDFQEEAKQYLQKTLKIYKDRNTFRNLGLSYRQSGDFEQAKKYFQLAIYIDPKYY
                     HAYHDLASLFV"
BASE COUNT          776 a          386 c          406 g          846 t
ORIGIN      
        1 tagggttcgc gaactcctga tacttttggg ttcgcgatgt gttgatattc gtcaactaaa
       61 tactagtaaa aaagaaagga aattaaaaaa atgtttatgt ttttccagaa aaacaaaaaa
      121 ggtttcacac ttattgaatt gatggtcgta gtagccatca tcggtatcct ggcgttgtta
      181 ggattgagat tataccggtc aacaagaaaa agccaaacaa gcaatcgtaa aagccaatgt
      241 tggtactgca cagacattag tgcaagctga aatggctgat ggtgctatat cgggtaatgt
      301 cgatggtgct gtaattattg ctgaaaaagg tgggatgcaa aatccatacg gtggcaccgt
      361 agtggttgta aaaagtggat ggactgcagt ggctggagct gtatgtataa ccccctgtgg
      421 cacagacgat ggaacatgtt ataaattcca aggttatgat aacagtgcta ctccaaaact
      481 atttggagat gaattaaccg ctcaaagata ataacagctc ttaatcacag gctcccttaa
      541 attctccttg attttaaggg agctttttgt ttatatatca aaaaatatga tatttataat
      601 aaaaatatat aactataaaa ttaaggggaa aataatttgg acaatattac tgacaatcgg
      661 atcgcttatc acaaaatatt gttaataatt ctcactatat ttattttact catttcttta
      721 tttgtctaca aagaagtcta cgagcgtcga attaatcaaa cattcattct gatgtcattc
      781 cttattattt tcttttttct aatcttaatt gaggtgttat catcaaaaag aattatatgg
      841 agaaaaagta aactaaacat agcgctgtgt tttttcatta ttatattaac tttctccctg
      901 tttagcggta gtaacattaa agtaaatttg agtcattatc tttatttcct attctatata
      961 tttttattat gtatggtatc ttatttgatt caatctatga aagaattcaa tttcattatc
     1021 aggatatttc ttgtgagctc ttctcttgtc gctctttata ccttgctgca atattatggg
     1081 ttcgatcctt actatggcta tcttagtcaa ttgacctcta ccatcggaca aaaaaactgg
     1141 ctctctaatt atttagcact catatttcca attacttttt cttttttctt attggaaacc
     1201 aacaggagat ataaaattat actctttctt ttattaacaa ttctttatac cgccttaatc
     1261 ataggtcaga gcaggggtat ttggataagt attataacaa ctttattatt ttcattagtt
     1321 tttatttaca gatacgggct atgggaagtt ttccggaata ataaaaaatg gcttattgct
     1381 ttactaacaa tatttttagt tgttactgtt atatattcca ctgagaatcc tctcaataag
     1441 agtagcttaa ctgtgattga aagagccgcc tctacttttg acgagaatga cccttccatc
     1501 aatactcgcc ttttaatctt ggaaacgact atggagatga ttaaaaataa accaatttta
     1561 ggtttgggta ttggtgcctt ctcctatcat tatctggatt atcaggcagc atatctggtt
     1621 aagcacccag aatatataaa atttagcggt aaagcggcag aagcacataa tgaatattta
     1681 catttagcgg cagagatagg cattgccggt ttgttttctt ttttaaccat aatttatatt
     1741 ttctattacc tgatttatca atatctggag aaagaaaaaa ataaaaacta taaaattatc
     1801 atttttggcc tattagcggg tgttacctgc tttcttatcc attgtctttt taccttccca
     1861 ttccatgtac cggtacttgg cacaacattt ttcattttat tggggttaac aattgcctat
     1921 atcaatctga ctgagaaagg taaagggaat gattctgcca aaaatattgt tcttattcac
     1981 tttaaattta atgctttata tagaacaata gcaatagtta tattaattgt actggcagta
     2041 accggattat ggaaaattgc tctaaaaccg tatctggcag aactctatta ttttaaaggt
     2101 gccagacatt ttgctgtaca taataatgaa gtagccttaa aatattttaa gaaagcggca
     2161 ctatatgatt ctaataatgg cagagttatg catgccttag gttcggctta ttaccagttg
     2221 gattttcagg aagaagctaa gcaatactta caaaaaacat taaagatata taaggacagg
     2281 aatacttttc gcaatctggg actatcctat aggcaatcgg gagattttga acaagcgaaa
     2341 aagtattttc agctggcaat ttatatagac cctaaatatt atcatgctta ccatgacctt
     2401 gcttcattat ttgt
//
LOCUS       DAOQWT010000235         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_49211_length_2407_cov_2.997874, whole genome shotgun sequence.
ACCESSION   DAOQWT010000235 DAOQWT010000000
VERSION     DAOQWT010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49211_length_2407_cov_2.997874"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PKJ95_05250"
     CDS             <1..941
                     /locus_tag="PKJ95_05250"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR65680.1"
                     /translation="DSQFLANIGDELLASIKNNITEQLINSILLYQFAQKEKINIPAS
                     KVNNEIDQIKKSFNSEQDFNNALKRNNLTLNQLKDSLNRQFMIDQVIQNEYDTISIPD
                     EEIAQYYEEYKNYFFQPERRKISHILVEDKEEAELLLSQLKEGTIEFEETAKEKSICP
                     SAENGGDLGYITRGQMVPEFEEASFALAAGNLSDIVETEYGYHIIKCDDIQEEKQLSL
                     EEAEENIKNILTSQKQNERVEELIARLREEADITILYDFSSEVEKKEESVDKGNDTLS
                     ETEINAEENQLSETPQENISENPVITEGSGEEKLTE"
     gene            complement(1040..>2407)
                     /locus_tag="PKJ95_05255"
     CDS             complement(1040..>2407)
                     /locus_tag="PKJ95_05255"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNR65681.1"
                     /translation="SKKPRYNGRITGRIFLGFVALILFFLLIYSIDSLIIYRGKVYPN
                     VIVMNQEIGGLKKDKAYQTIQPIILENLREPILISYNGWRLNIVPEDNLGATVNLEKL
                     INDAYSIGRRGSLLSRIKERIYLIGNACRLTPSIEFKDNNFQSFYYDIQMEIEKPSQD
                     ASFSSDKIIPAQIGIKIDREQLLTALKRDIIDSARSDIPTVIDLPVHYQEPKISTREI
                     LSQIGVVEALSSYETSLLGKEKNTLFNIQKSAADINGIILKPGETLSFNRLIGPADKE
                     DGYKESTIIANGQFIKGYGGGVCQVSTTLYNAALLANLQIMERYNHSIYGKPTSYVPL
                     GQDAAVFYGYKDLKFKNSLDQQVVIFSEIKPDRLIVTIWGEKGLDKEIRIINQDLKTY
                     DYDVIEIKREGQDTTHLVGNVLQEGIPGYSVKTYRLIVDSSGEKMEFLSEDRYISVPG
                     KILID"
BASE COUNT          797 a          419 c          458 g          733 t
ORIGIN      
        1 atgacagtca atttctggct aatattggcg atgaactgtt agcctctatt aaaaacaaca
       61 ttactgaaca attaataaat tctatcctgt tgtatcaatt tgctcagaaa gagaagataa
      121 atattccggc aagcaaagta aataatgaga tagatcaaat taaaaaaagc tttaattccg
      181 aacaagattt taacaatgct ttgaaaagaa ataatcttac cctaaaccag ctgaaagaca
      241 gtttaaacag acaatttatg attgaccagg ttatacagaa tgagtatgat actatttcta
      301 ttcctgatga agagattgca caatactatg aagaatataa gaactatttc tttcagccgg
      361 aaaggagaaa gataagtcat attctggttg aggataagga agaagcagag cttttactga
      421 gccagctgaa agagggaaca attgaatttg aagaaacagc taaggaaaaa tctatttgcc
      481 cttccgctga aaatggtgga gatctggggt atattacccg ggggcagatg gtccctgaat
      541 ttgaggaggc ttcttttgcc ttagcagcag gaaatttaag tgatattgtg gagactgagt
      601 atggttatca tattattaaa tgtgatgata tacaggagga aaagcaacta agtttggaag
      661 aggctgaaga aaatataaaa aatattttaa ccagccagaa acagaatgag agagtagaag
      721 aactgatagc ccgattaagg gaagaggccg atattactat tctctatgac tttagctcag
      781 aggtagagaa aaaagaagag agtgtagata aaggcaatga tacactatcg gaaactgaga
      841 ttaatgcaga agaaaatcaa ttatctgaaa caccccaaga aaacatttct gaaaatccgg
      901 taataactga aggtagcgga gaagaaaaac tgactgaata agcagatatt tataaagcta
      961 agtttggaat aaaaacaatc ttccttcagg tcaatattgt tatccaatat ttcaatggtt
     1021 taatagccgg acagatcagt caatctatca atattttacc gggtacgcta atatagcgat
     1081 cttcagagag aaattccatt ttctctcctg aagaatctac tattaggcga taagtcttga
     1141 cactatagcc aggaatccct tcctgcagaa cattaccgac taaatgtgtt gtgtcctgcc
     1201 cttctctctt aatttcaatt acatcataat cgtaagtttt tagatcctgg ttaattattc
     1261 gtatttcctt atccagcccc ttttctcccc agatagttac tataaggcga tccggtttaa
     1321 tctcactgaa gataacgacc tgctggtcta aagaattttt aaattttaaa tctttatatc
     1381 cgtagaatac tgcggcatcc tgtcccagag ggacatagct ggttggctta ccatagatag
     1441 agtgattgta tctttccata atctgaaggt tcgccagcag tgctgcattg tacagggtag
     1501 tggatacctg acaaacacca ccaccgtatc ctttaataaa ctgtccgttg gcaataatag
     1561 tactctcttt atagccatct tctttatcgg caggacctat taaccgattg aaagaaagag
     1621 tctcccctgg ttttaagatg ataccattaa tatctgctgc tgatttttgt atattaaaaa
     1681 gagtattttt ttctttaccc aacagggaag tttcatagct gctcagggcc tcaacaactc
     1741 caatttgtga tagtatttct ctggtagaaa tctttggttc ctggtaatga acaggtaggt
     1801 caataacagt cgggatatcg gatcgggcag aatcaattat atccctttta agagctgtta
     1861 ataactgctc ccggtcgatt tttatgccaa tctgagcagg aattatttta tctgaggaga
     1921 aggaggcatc ctgagagggt ttttctatct ccatctgtat gtcataataa aacgattgaa
     1981 aattattgtc tttaaactct atggagggag tgagccgaca ggcatttcca atcaaatata
     2041 ttctttcttt tattctagat aagagcgaac ctcttctgcc aattgaataa gcatcattaa
     2101 taagtttttc cagattgaca gtagcaccaa gattatcttc gggtactata ttcaatctcc
     2161 atccattata acttatcagt attggctccc ggagattttc taatatgatt ggctggatgg
     2221 tctgataggc cttatccttc tttaatcctc caatttcttg attcataaca attacattgg
     2281 gatatacttt tccccggtat attattaatg aatcaatgga atagatcagt aaaaagaata
     2341 gaatgagagc aacgaaacct aaaaaaatcc tgccggtaat tctgccattg tatctgggtt
     2401 ttttgct
//
LOCUS       DAOQWT010000236         2405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_49271_length_2405_cov_3.504681, whole genome shotgun sequence.
ACCESSION   DAOQWT010000236 DAOQWT010000000
VERSION     DAOQWT010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49271_length_2405_cov_3.504681"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PKJ95_05260"
     CDS             complement(<1..478)
                     /locus_tag="PKJ95_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNR65682.1"
                     /translation="MEKQELKYWLAWNRVKEIGPIRFNKLYQHFSSLEEAWNSNIKKN
                     DMMNLLHIREEIFNRIEREKEQINPDEEWDLLQKRNIRVVTIKEKEYPALLKNIYNPP
                     PVIYYQGNFTGIMKEAKGIAIVGSRKATFYGRKVSREIAGEMAKSGYIVISGLARGI"
     gene            complement(513..1055)
                     /locus_tag="PKJ95_05265"
     CDS             complement(513..1055)
                     /locus_tag="PKJ95_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNR65683.1"
                     /translation="MSNNEDRLTSLLIRILLIIMFSLGAVLLGGYAAFLAYQTIFAVP
                     IVQVPSVIDMELESARQTLYKTGLKISTIDDGVFQKGERYIVIAQKPPAGTRLKKNRT
                     VEVEIRMTNMYRQIPDLFGKTVAEAEILLSEHGYQIGDIAYTLHQKLPEGRIIAQTPP
                     AGESQTSDSKVNILVSKGLY"
     gene            complement(1067..>2405)
                     /gene="rsmB"
                     /locus_tag="PKJ95_05270"
     CDS             complement(1067..>2405)
                     /gene="rsmB"
                     /locus_tag="PKJ95_05270"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNR65684.1"
                     /translation="EILERVEKQNSYLNILLPSFYARYKPNDPDKALMQEISYGVTRF
                     KKRLDWIIAQYLTSRIKKIPLTVQNILRMGAYQILYLEKIPAYAIVNESVELVKDSSY
                     STYSGMVNAILRNISRKSVLVSWPDIESEPVRYISVFYSYPEWLVERWMKRFGLEICL
                     AICQAGNEKPDFALRINSLRISQEQFQRKLSELKIPFQKSLYLPQCAITIKDHYNIVN
                     MPFFQDGLFSIQDESSMLASEFLMPGAGETVFDMCSGPGGKTTHMAQIMGNKGKIVSF
                     ENNRRRLEMVMAESRRLGIKIVSPVLADSRRLNNEYLEKADKILLDAPCSGSGVIRKK
                     PDLKWKKWNKEHLRKLNQIQKELLDTAARYLRWGGELLYATCSMEREENDGIILNFIK
                     EYSHFTIQDSSQFVEKNGIVKYKTEIEGAIQLIPGYSGANIDGFYMVKLKKGK"
BASE COUNT          688 a          529 c          376 g          812 t
ORIGIN      
        1 caatgcccct ggccagcccg cttataacaa tataaccaga tttagccatt tctcccgcta
       61 tttcccggga tacctttcgt ccgtaaaaag tagcctttct ggaacccact atagctatac
      121 cttttgcttc tttcataatt ccggtaaaat ttccctgata ataaataacg ggcggcggat
      181 tgtatatatt ttttaacagg gcaggatatt ctttctcttt gatagttacc actctgatgt
      241 ttctcttttg cagcaaatcc cattcctcat cgggattaat ctgttctttt tcccgctcaa
      301 ttctattaaa gatttcttct ctgatatgta gcaaattcat catatcattt tttttaatat
      361 tagaattcca ggcctcttcc agagaagaaa aatgctgata aagtttgtta aaacgtatgg
      421 gacctatctc tttaactcta ttccaggcta accaatattt tagttcctgt ttttccattt
      481 tttctaacct tacacttgaa ttattttatt tatcaataca atcccttgct taccaggatg
      541 ttgaccttgc tgtcactggt ctggctttct cctgccggtg gtgtttgagc aataatcctg
      601 ccttcgggta acttctggtg caaggtgtaa gcaatatctc ctatctgata accatgttcc
      661 gaaaggagta tctcagcttc agcaacagtc ttgccaaaca gatcaggaat ttgtctatac
      721 atgtttgtca ttctgatttc aacttctaca gtgcggttct tttttaatct ggtgccggca
      781 gggggtttct gggcaataac tatatatcgt tccccctttt gaaatacacc atcatcaata
      841 gttgaaattt ttaaacctgt tttatataag gtttgacgtg cagattctaa ttccatatca
      901 ataactgaag ggacttgaac aatcggaaca gcaaaaatag tctgataggc aagaaaagca
      961 gcatatcctc ccaaaagtac agctccaagg ctgaacatta taatcaacaa aatacggatt
     1021 aacaagctgg tcagcctgtc ttcattgtta ctcataatgt tttccttcat ttgcccttct
     1081 tcaactttac catataaaat ccatcaatat tggcaccaga atatcctggg attaattgaa
     1141 tggccccttc aatttctgtt ttatacttta ctataccatt tttttccaca aattgtgaac
     1201 tatcctggat ggtgaaatgg gaatattcct tgataaaatt taaaattata ccatcattct
     1261 cttctctctc catactacag gttgcataaa gcaattctcc gccccatctc aaataacgag
     1321 cagccgtatc gagcagctct ttctgtatct gatttaattt acgaagatgt tccttattcc
     1381 actttttcca tttaaggtct ggtttttttc tgattactcc gctaccggag catggagcat
     1441 caagtaatat tttatcagcc ttttccaaat attcattatt taatctacgg gagtccgcca
     1501 atactggtga aacaatttta ataccaagtc tcctggattc tgccataacc atttccagcc
     1561 ttctccggtt attttcgaaa gagacaattt tacctttatt tcccataatc tgagccatat
     1621 gggtagtttt ccctcccggc ccactgcaca tatcaaaaac tgtttcaccg gcaccaggca
     1681 tcaaaaactc tgaagccagc attgaacttt catcctgaat ggaaaataaa ccatcctgaa
     1741 agaagggcat attaacaata ttataatgat ctttaatggt aatcgcacac tgtggcagat
     1801 ataagctttt ctgaaaaggt atctttaatt cagataattt tctctggaat tgttcctgac
     1861 ttattctcag agaatttatc ctcagagcga agtcaggctt ttcattgcca gcctgacaga
     1921 tagccaaaca aatctccaga ccaaatctct tcatccatct ttctactaac cattctggat
     1981 aagaataaaa aacagaaata taccggaccg gttctgactc aatatcaggc cagctgacaa
     2041 ggacagattt tctggaaata ttccttaaga tggcatttac cataccggaa tacgttgaat
     2101 aagaactgtc tttaaccagc tctaccgatt cattcactat ggcataagcc ggtattttct
     2161 ccaaatacaa aatctgataa gctcccatcc tcagtatatt ttgaacagtc agggggattt
     2221 ttttaattct gctggtaaga tattgagcta taatccaatc caatctcttt ttaaaacggg
     2281 ttactccata acttatttcc tgcattaagg ctttgtcagg atcatttggt ttatacctgg
     2341 cataaaaaga ggggagtaga atgtttaaat aagaattctg tttttccacc cgttctaaga
     2401 tttct
//
LOCUS       DAOQWT010000237         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_49412_length_2401_cov_2.866581, whole genome shotgun sequence.
ACCESSION   DAOQWT010000237 DAOQWT010000000
VERSION     DAOQWT010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49412_length_2401_cov_2.866581"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..2032)
                     /locus_tag="PKJ95_05275"
     CDS             complement(23..2032)
                     /locus_tag="PKJ95_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HNR65685.1"
                     /translation="MIDLNKIDGKQRVVIENVQPEIDNGLFAIKRIEGESVKVSANIF
                     ADGHDRIQAYLLFRKKGECSWQKTSMEMIAKDIWQGEFTVTELGIYEYTIEALVDHLA
                     TWWEDIQKKATGGDNLIVDLKCGLSLLAKIINEVEETGKKASIQKLITLIQKGEKREQ
                     EEILRLLEKELDNEFFKKYHLPDHITRYDKTLRIAVDRKRAGFSAWYELFPRSCSAKP
                     GVHGTFQDCLSRIPEIAGMGFNVLYFPPIHPIGKTHRKGKNNAIIANADDPGSPWAIG
                     SEIGGHTVIHPQLGSLADFREVIKKAEEHGMEIALDIAFQCSPDHPYIKEHPEWFRWR
                     VDGTIQYAENPPKKYEDIVPFNFETEHWKELWEELKNVVLFWIKEGVKIFRVDNPHTK
                     PFLFWEWLISLIKEDYPEVIFLSEAFTRPKVMYRLAKLGFTQSYTYFTWRNTKQEYTD
                     YLNELAHTQLKEYFRPNFWPNTPDILSEYLQTGGAPAFIIRFLLAATLSSNYGIYGPP
                     YERFINTPLLGKEEYCDSEKYEIKHWLPDEVKESTRELFTTVNRIREENSEFHRTDNI
                     RFLAVENNQLLYFAKFNDEENDAILVVINLDPNYTQSGWIKVPLYELGISEEQTFLAH
                     DLLGGGQYIWQGEYNYVELNPHILPAHIIKIKRHLKQEDQFDYFS"
     gene            complement(2092..>2401)
                     /locus_tag="PKJ95_05280"
     CDS             complement(2092..>2401)
                     /locus_tag="PKJ95_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65686.1"
                     /translation="IIGEGGFEMVAVNKKIDVYQEAPTMEQYFYLHNGIPIKNLAELI
                     DQLVDMGQELFNHHVNPENNDFANWIRDVFGEKELARRLKMSRSAQGMLKSITRYLES
                     "
BASE COUNT          668 a          489 c          435 g          809 t
ORIGIN      
        1 gctgaacata ttattattcc tcctaactaa aatagtcaaa ttggtcttct tgtttgagat
       61 gtcttttaat ctttataata tgagctggta aaatatgtgg gtttaactct acataattgt
      121 attcaccctg ccagatgtat tgcccgccac ctaataaatc atgggctaaa aaggtttgct
      181 cttctgaaat acccagttca tagaggggaa ctttaatcca tcctgattgt gtataattcg
      241 ggtctaaatt gattactacc agaatggcat cattttcttc atcattaaat ttagcaaaat
      301 agagcaattg attgttttct accgctaaaa atcggatatt atcggttctg tgaaattcag
      361 aattttcctc tctgatacgg ttaacagtgg taaatagctc tctggtactc tcttttacct
      421 catcaggcag ccagtgtttt atttcatatt tttcagaatc acaatactcc tctttaccga
      481 gtaagggtgt atttataaat cgttcatatg gtggtccgta gataccataa ttagaagaga
      541 gggttgcagc cagcaaaaat cttattataa aagcgggagc accaccggtc tgtaaatatt
      601 cagaaagtat atcaggtgtg ttaggccaga aattgggacg gaaatattct ttcagctgag
      661 tatgggccag ttcatttaaa tagtctgtat attcctgttt ggtattacgc caggtaaaat
      721 aggtatagga ttgggtaaaa ccaagtttgg ctaatctgta cattactttt ggtctggtaa
      781 aggcttctga taaaaagatg acctcaggat aatcttcttt tatcagactt attagccatt
      841 cccagaatag aaatggtttg gtatggggat tgtctactct aaaaatttta acaccctctt
      901 taatccagaa aagaacaaca tttttcagct cttcccacag ttctttccag tgttcagtct
      961 caaaattaaa agggacgata tcttcatatt ttttaggtgg gttttcagca tattggatgg
     1021 tgccatcgac ccgccagcgg aaccattcgg gatgttcttt gatataagga tggtctggtg
     1081 agcattgaaa ggcaatatct aaagctatct ccattccatg ttcctctgct ttcttgataa
     1141 cctctcgaaa atcagccaga cttcccagct ggggatggat aacagtatgt ccccctatct
     1201 cactgccgat tgcccaggga ctgcccgggt catctgcatt ggctataata gcattattct
     1261 ttcccttgcg atgtgttttt ccaataggat ggataggtgg aaaatagaga acattaaatc
     1321 ccataccggc aatttcggga atccggctca gacaatcctg aaaagtgcca tgaacaccgg
     1381 gtttagcaga acaggagcgg ggaaagagtt cataccaggc gctgaaaccg gctctctttc
     1441 tgtctactgc tattctcagg gttttatcat aacgggtaat atggtcgggc aggtggtatt
     1501 ttttaaaaaa ttcattatcc agctcttttt ctaatagtct tagtatttct tcctgttccc
     1561 tcttttcacc cttttggata agtgtaatga gcttttgaat tgaagctttt ttgccagtct
     1621 cttcgacctc attgattatc ttagccaata gggagagacc acactttaaa tcgacgatca
     1681 gattgtcacc accggtggct tttttttgaa tatcttccca ccaggtagct aaatggtcta
     1741 ccagtgcctc tatagtatat tcataaatgc cgagctcggt tacggtaaat tcaccctgcc
     1801 aaatatcttt ggcaatcatc tccatagaag ttttttgcca ggaacattcc ccttttttcc
     1861 gaaaaagcag ataggcttga atacggtcat gaccatcggc aaatatattg gctgatacct
     1921 taacactttc accttctatt ctttttatgg caaataaacc attatcaatt tcaggttgaa
     1981 cattttcaat gacaacccgt tgttttccat caatcttatt taaatctata attttttaat
     2041 cctctctact atctcttcaa tttaatattc actaaccgaa aaacagaaaa attatgattc
     2101 taaatatctg gtgattgatt tcagcatacc ctgagcggag cgggacattt ttagtcttct
     2161 ggctaattct ttttctccga atacgtccct tatccagttg gcaaaatcat tgttctctgg
     2221 attgacatgg tgattaaata attcctggcc catatcaacc agctggtcaa ttaattctgc
     2281 caaatttttt attggtatgc cattgtgcag gtagaaatat tgctccatgg ttggcgcttc
     2341 ctgatagaca tcaatttttt tattaacagc aaccatttca aatcctcctt cccctataat
     2401 c
//
LOCUS       DAOQWT010000238         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_49743_length_2391_cov_2.091610, whole genome shotgun sequence.
ACCESSION   DAOQWT010000238 DAOQWT010000000
VERSION     DAOQWT010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49743_length_2391_cov_2.091610"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PKJ95_05285"
     CDS             complement(<1..619)
                     /locus_tag="PKJ95_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65687.1"
                     /translation="MSKGDIWWTNSIRKKMLIYLLIFFTSFISVFISLPLVIPLLEKA
                     GIYGKNQNKPENESIAEMGGLIMVLGFGLGLILVIALKTFFNLFPSVPLVTILTVLSV
                     ELFIIIIGTIDDLISMPQLVKAIIPALASLPLIALEAGHTTMIIPFVGKIDFGLFYTL
                     FLLPMGMTGAVNAVNMLAGFNGLEIGMGLVMFISLLILSIIKSKIT"
     gene            complement(621..767)
                     /locus_tag="PKJ95_05290"
     CDS             complement(621..767)
                     /locus_tag="PKJ95_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65688.1"
                     /translation="MIKCFFESKNQEDMIKKYIRGYKEIPEEVGDIAELEKEEYQVLE
                     KDFQ"
     gene            complement(954..2219)
                     /locus_tag="PKJ95_05295"
     CDS             complement(954..2219)
                     /locus_tag="PKJ95_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR65689.1"
                     /translation="MKNSILVIEQHFYPEVAATGQLLLDLCEDLVKAGYQVKVVTGNP
                     TEIYPKQMNVKRRENYKGIEIVRLRNTRLSKYNMAGRIINYLTFHLFILFNLIFSQRP
                     DLVIVLSTPPFIAIHGVILKVFKNCKVIYNIQDLFPELAVQLNKLKNETFISVLKSLS
                     RYIIGKMDRIVVVGEYMEKKIKKEIIKDCKIGQQISIIHNWADGKEIKVIDNNDKSNY
                     FKKEWGLENKFVVLYSGNIGYLHEFDTIIDAADSLQKKGHNDILFVFVGEGIKKEYIK
                     NEINKRELNNFLFFPYQPRENLTYSLGLADVSLVTLEKGFEGMVVPSKIYGILASGRP
                     VIGIVGEYSEITDIIRRAKCGKVVQIGDAQFLEDCIMEYYNDSKKSREDGLNGRKYFE
                     KHFDRAIATEKYISLIKRTVTRMVILIYF"
BASE COUNT          780 a          470 c          275 g          866 t
ORIGIN      
        1 ctgttatttt tgatttgata attgatagaa ttaaaaggct gataaacatg accaatccca
       61 ttccaatttc cagtccatta aaaccagcca gcatattgac tgcatttact gctcccgtca
      121 ttcccattgg taataagaat aatgtataaa acaatccaaa atcaatttta ccgacaaagg
      181 gtattatcat agttgtgtga cctgcctcta aagcaattag gggaagagat gccagtgccg
      241 gtatgattgc tttgacaagc tgaggcatag agatcaaatc atcaatagtg ccaataatta
      301 ttataaatag ttctactgat aaaacagtca aaatagtaac caggggtact gaaggaaata
      361 gattaaaaaa ggttttcaaa gcaattacca gaataaggcc cagaccaaac cccagtacca
      421 taatcaatcc acccatttcg gcaatacttt cattttcagg cttattctgg ttttttccat
      481 aaatacctgc tttttctaat aagggaatga ccaggggtag tgaaataaaa acactaataa
      541 aggaagtaaa aaaaattaat agataaatta gcattttttt tctgatggag ttagtccacc
      601 aaatatctcc ttttgacatc ttattgaaaa tccttttcca ggacttgata ttcttctttt
      661 tctaattcag ctatgtcgcc aacttcttca ggtatttctt tataacctct tatatatttt
      721 ttaatcatat cttcctggtt tttactttca aaaaaacatt taatcatacc ttctattgga
      781 gcgttcctat tattatttct tttccttttt cattttccaa ttttttaaaa ctattttcat
      841 caatagtgat agtgaaacga ataccagtaa ttcttccagc ccatttaaaa aataatctat
      901 aaatattaaa ataaaaagaa atattttata ttcaactgtt atgctattgc ttgttaaaag
      961 tatataagaa taaccatcct tgtgacagtt ctttttatta gacttatata tttctcagta
     1021 gcaattgccc tatcaaaatg cttctcgaaa tattttctac catttaatcc atcttccctg
     1081 ctctttttac tatcattata atattccata atgcaatctt ctaaaaattg tgcatctcca
     1141 atctgcacaa cctttccaca ctttgctctt cttataatgt cagtaatttc actatactct
     1201 cccacaattc cgattacagg tcttccgctg gctaaaatac cataaatttt acttggaact
     1261 accattcctt caaatccttt ttccaaggtt accagagaaa catctgctaa acccagacta
     1321 tatgttaagt tttcccgtgg ttgataagga aaaaataaaa aattatttaa ctctctttta
     1381 ttgatctcat tctttatata ttctttctta ataccctccc ccacaaaaac aaaaagaata
     1441 tcattatgcc cttttttttg taagctatct gctgcatcta ttatagtatc aaattcatga
     1501 agatagccaa tattacccga atataagaca acgaacttat tttccaaacc ccattctttt
     1561 ttaaaataat tacttttatc attattatca atgaccttaa tctcttttcc atctgcccaa
     1621 ttgtgtataa ttgaaatttg ttgccctatt ttacagtcct ttattatctc cttttttatc
     1681 ttcttttcca tatattcccc aaccactaca attctatcca tcttaccaat aatatatctg
     1741 gataaacttt ttaaaactga gataaaggtc tcatttttta atttgttcag ttgtacagct
     1801 aattcaggaa ataaatcctg tatattataa ataaccttac aattcttaaa tacttttaaa
     1861 atgactccat gaatagcgat aaaaggagga gtggataaaa ctattaccaa atcaggtctt
     1921 tgagagaaga ttaaattaaa taaaataaat aaatgaaaag ttaaataatt gatgattctg
     1981 ccagccatat tatatttact caaccgcgta tttctcagac gaacaatttc tattccttta
     2041 taattttccc tccttttaac attcatttgt tttggatata tttcggtagg atttccggta
     2101 acaaccttaa cctggtatcc tgctttcact aaatcctcac ataaatccag caacaattgg
     2161 ccggtagctg ccacttcagg ataaaaatgc tgttcaataa ccaaaatgct atttttcaat
     2221 gattataatt aatcctttcc aaggtctttc tgttttccct gtaccattct atggtcattt
     2281 ttaatccttt ttgaaaatcc atgtcagctc gaaaaccaaa ttccttctca gctttattta
     2341 tatccaatct tctccggggt tgtccatcgg gtttgctatt atcccaaata a
//
LOCUS       DAOQWT010000239         2386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_49885_length_2386_cov_3.155298, whole genome shotgun sequence.
ACCESSION   DAOQWT010000239 DAOQWT010000000
VERSION     DAOQWT010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49885_length_2386_cov_3.155298"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PKJ95_05300"
     CDS             <1..219
                     /locus_tag="PKJ95_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HNR65690.1"
                     /translation="PILILALPLFDTLFAIWRRCRSHKPIFQADQGHLHHLLLKRGLS
                     HRNVVLFLLSISAICNLIAIGIVWLCLK"
     gene            279..1250
                     /locus_tag="PKJ95_05305"
     CDS             279..1250
                     /locus_tag="PKJ95_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNR65691.1"
                     /translation="MKEKVLVTGGAGFIGSHIVDLLIDYGYELAIIDDLSSGKTENIN
                     NKARFYQVDINNSEVEEVFEREKPDYICHQAAQISVSYSVKEPKSDAQSNIMGLLNLL
                     ELSVKNGVRGFIFASSGGTVYGESKSFPITENVPLFPTSPYGISKMTSEYYLHYYCQN
                     HNLKYITLRYGNIYGPRQDPFGEAGVIAIFIRKMLKGDIPIINGDGEYVRDYVYVKDV
                     TQACLLGIKNMLKLTEIKKKRKKDTIFNAYNIGTGMGISVNQLYSYLQEIIGFKKEAI
                     YGPPRSGDLRKNVLDCHLAHQILGWEVRFTLEEGLKETVEWFKMRCC"
     gene            1321..>2386
                     /locus_tag="PKJ95_05310"
     CDS             1321..>2386
                     /locus_tag="PKJ95_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65692.1"
                     /translation="MAEWVNFLIRIAGILFAGVLMILLGKYNQKKKLTSWLKLFFQII
                     IALIIITAGVKIEFLRNYSDGYWYLNQLSIPITIIWLITITNSIGQTDELGDITPITV
                     LIAALTFFVVSIFQRQGLIIAEILSSFLAVFSIVIIFLKKKSVSKKNNTFSSYYMFFG
                     FMLAIIAIVGVLKSTAALTLLTPLLILGFPIVDTSYSFIASFLSDNYLGNVSESKLRQ
                     QLIEQGFSWKGANMIIIATCIYLSVIAAIVSVKENLFLFLTMIGMGYLAYYWLRHRVS
                     NGLSIINVDESGKKVEFFGVPIDRINCHQALERIEWFIRTKEAHFIITPDTLAILRAR
                     KDKQYLEIAKKTRDFMVKNMK"
BASE COUNT          790 a          335 c          435 g          826 t
ORIGIN      
        1 cctattctta ttttggctct acccctattt gatactcttt ttgctatatg gcgaagatgc
       61 agaagccata agccaatatt tcaggctgac cagggccatt tacaccattt attgttaaaa
      121 cgaggtcttt cccatcgtaa tgttgtttta tttttattat caattagtgc aatttgtaat
      181 ttaatcgcta taggtattgt ctggctttgc ttgaaataat ttttttaaaa agaatatact
      241 gatatcaata atttaataag aagtgagaga tgtatacaat gaaagagaag gtattagtta
      301 ccggaggtgc cgggtttata gggtcgcata ttgttgattt actaattgat tatggttatg
      361 aacttgcaat tatagatgat ttatcttctg ggaagacaga aaatattaac aacaaagcgc
      421 gtttttatca agtagacatt aataattctg aagtcgaaga agtatttgaa agagaaaaac
      481 ctgattatat ttgtcatcaa gcagctcaga taagcgtttc ttattcggtt aaagagccta
      541 aatcagatgc tcagagtaat attatgggat tattaaattt actggaacta tcagttaaaa
      601 atggggtaag aggttttatc tttgcctcaa gtggcggtac tgtttacggt gaaagcaaat
      661 catttcctat tacagaaaac gtcccacttt ttcccacttc tccttatgga atttccaaaa
      721 tgacatctga atattattta cattattatt gtcagaatca taatttaaaa tatattactt
      781 tacgctacgg caatatctat ggtccaagac aagacccatt cggtgaagca ggagttatcg
      841 ctatttttat aagaaaaatg ctaaaaggag atatacctat tattaatgga gatggagaat
      901 atgtcagaga ctacgtttat gtgaaagatg ttactcaggc ttgtttactc ggcattaaaa
      961 atatgttaaa attaactgag ataaaaaaga aaagaaaaaa agatacaatc tttaatgcct
     1021 ataatattgg aacaggtatg ggaatatcag taaatcaact gtactcttat ttacaagaaa
     1081 taattggatt taagaaagag gcaatttatg gacccccccg ttcgggagat ttaagaaaaa
     1141 acgtccttga ttgtcatctg gcacatcaaa tattgggatg ggaagttcgt tttactttag
     1201 aagaaggatt gaaggaaaca gtagaatggt ttaaaatgag gtgttgttaa tagtaaagaa
     1261 gtataactgt ggttttagaa ccagtttatt gaaatttttt aaaaatttag ggaggctttc
     1321 ttggctgaat gggtcaattt tctgattagg attgccggta ttctttttgc tggggtctta
     1381 atgattttat tagggaaata taaccagaaa aaaaagctta cttcctggtt aaaattattt
     1441 tttcaaatta ttattgcact tataattatt acagcaggag ttaaaataga gttcttaaga
     1501 aattattctg acggatactg gtatctcaac caactttcca tacccattac cattatctgg
     1561 ttaattacca tcactaattc gatagggcaa actgatgaat taggagatat tactcccatt
     1621 accgttttga tagctgcttt aacatttttt gttgtttcta tttttcaacg acagggtttg
     1681 ataattgcag aaatattgtc atcattttta gctgtttttt ctatagtaat cattttttta
     1741 aagaaaaaaa gtgtttcaaa aaagaataat accttttctt cttattatat gttttttgga
     1801 tttatgttag ccatcattgc tattgtagga gtattaaaaa gcactgctgc tctaactctc
     1861 ttaacaccac ttttaattct ggggttccct atagtggata cctcctattc ttttattgcc
     1921 agctttctaa gcgataatta tcttggaaat gttagtgaaa gtaaattaag acaacaactg
     1981 attgaacagg ggttttcctg gaaaggtgcc aatatgatta ttattgccac ctgtatttat
     2041 cttagtgtta ttgcggcaat tgtatctgta aaagagaacc tgtttttatt cttaactatg
     2101 attggtatgg gatatctggc ctattattgg ctaagacata gggtttcaaa tggactgagt
     2161 ataatcaatg tagatgagtc tggcaaaaaa gttgaatttt ttggtgtacc aattgatcgt
     2221 attaattgtc atcaagcact tgaacgtata gaatggttca tcaggacaaa agaggcacat
     2281 tttataataa ccccagatac tctggccata cttagagcca ggaaagataa gcaatatctg
     2341 gaaatagcca aaaaaacaag agatttcatg gtaaagaata tgaaat
//
LOCUS       DAOQWT010000240         2384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_49956_length_2384_cov_2.936883, whole genome shotgun sequence.
ACCESSION   DAOQWT010000240 DAOQWT010000000
VERSION     DAOQWT010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49956_length_2384_cov_2.936883"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="PKJ95_05315"
     CDS             <1..132
                     /locus_tag="PKJ95_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNR65693.1"
                     /translation="NGSGIAVGRTMVAILENYQEQDGSIVIPDALRSYMNGLHKIPS"
     gene            181..266
                     /locus_tag="PKJ95_05320"
     tRNA            181..266
                     /locus_tag="PKJ95_05320"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:213..215,aa:Ser,seq:tga)
     gene            321..1139
                     /gene="murI"
                     /locus_tag="PKJ95_05325"
     CDS             321..1139
                     /gene="murI"
                     /locus_tag="PKJ95_05325"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNR65694.1"
                     /translation="MREKEKAIGLLDSGLGGLSVFRELKKQLPLEQVIYFGDTAHAPY
                     GEKSDEEILSYVISIIDFLLEQEVKLIIMACNTATAVALDKIKKRYSIPIIGVIQPGA
                     QEAVNKTKNKRIGIIGTEVTIRNKSYEKIIKNIDPSITTYSNACSNQVIREMEEQSLS
                     NENKIKLLLKECIKPLIKSGIDTLIWGCSHYPFLRKYMEEEIGHNTVLVDPAQATVKE
                     ARQWLVQNDLNNDIAINKEDIFYISGNPDLFARIAQKLLGYSPGKFLKPFNYNY"
     gene            1205..1351
                     /locus_tag="PKJ95_05330"
     CDS             1205..1351
                     /locus_tag="PKJ95_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65695.1"
                     /translation="MDNNLLDEFNIDNNKQDRMIIFDGTDKPFLLFYLIIFILGIILT
                     LIAI"
     gene            1374..>2384
                     /locus_tag="PKJ95_05335"
     CDS             1374..>2384
                     /locus_tag="PKJ95_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65696.1"
                     /translation="MSIQFTILIIYFIFIIFLGVLAVRKVKASDDLLVAGRNLGLMYV
                     SVSIAAEYMGGLGTIGTAEVAFSQGMGVIWYHIASSFGLILFGLAFAHYYRKYNVITV
                     PEFLYYLYDIKTWKAASVLNVVGYWFFTIIQMTALGALVTSVTGLDLKLSVLICGIAM
                     TIYLLAAGMWSVAFTSVAFMLTIGTGIPIAFWWVMKKEIPLLAASAGLSGYSGLAAAL
                     NGMGLDATALFSPFSLGGMAVFGYFIGGILGIPAAQATINYSFGAKNWKIARLAPVLA
                     AILILPLSIWTGSMGLFARAAGLTNNPKLALGATLMSIHPIVGGIGIAGVFAALISTV
                     AGI"
BASE COUNT          704 a          394 c          514 g          772 t
ORIGIN      
        1 aacggttccg ggatcgcggt aggcaggact atggtagcta ttttagagaa ttatcaggaa
       61 caggatggtt ctatcgtaat tccggatgcc ctgcgttcct atatgaatgg attgcataaa
      121 attcccagtt gaggtagaat tttactgcac atatatttta atatttttta aactgtttgc
      181 ggagggatgt ccgaatggca aggggccggt cttgaaaacc ggtgtaggcg taagctgtct
      241 gggttcgaat cccagtccct ccgccatatt ctatttagcc acttttttat aaaacatttt
      301 catataagag ttgtttgaag atgagggaaa aagagaaggc aataggttta ttggactctg
      361 gtttgggtgg tttaagtgtt tttagggaac taaaaaaaca gcttcccctg gaacaggtta
      421 tctattttgg agacaccgcc catgctccat atggtgagaa gagcgatgaa gagatccttt
      481 cttatgtaat ctctattatt gactttttac ttgaacagga agtcaaacta atcataatgg
      541 cctgtaatac tgctactgca gtggcattag ataagatcaa aaagagatac agtattccca
      601 ttataggggt aatccaacca ggtgctcaag aggcggttaa taaaacaaaa aataagagaa
      661 ttggtattat tggtacagag gtaactattc gcaataagag ttatgaaaaa ataatcaaaa
      721 atatagaccc atcaataact acctacagca atgcctgcag taatcaggtt ataagagaga
      781 tggaagaaca gtctttaagc aatgagaata agattaaact cttgctgaaa gaatgtataa
      841 aaccattgat aaagagtggt attgatacac ttatctgggg gtgttcacac tatccttttt
      901 taagaaaata tatggaagag gagataggcc ataatacagt tctggtggac ccggcccaag
      961 ccacagtaaa agaagccagg caatggcttg ttcaaaatga tttaaataat gatattgcta
     1021 ttaacaagga agacatattt tatattagtg gcaatccgga tttatttgcc cgtattgccc
     1081 aaaaattgtt aggatattcc ccaggtaaat ttcttaaacc tttcaattac aattattgat
     1141 atttattttt ttaataactt atctgggaaa aattaaataa ttaaaaaata ttcagtgggt
     1201 aattatggat aataatctat tagacgaatt taatattgac aataataaac aggataggat
     1261 gattatattt gatggaacag acaaaccttt tctacttttt tatctgataa tctttatcct
     1321 gggtattatt cttaccctga tagctatcta aaaaagagga agggaaaaaa gccgtgtcca
     1381 ttcagtttac cattctgata atctatttta ttttcattat ttttttaggg gttttagcag
     1441 tacgtaaggt taaagcctct gacgatttac tggttgccgg gcgtaattta ggactgatgt
     1501 atgtgtcagt atccattgca gcagaatata tgggtggact gggaacgata ggcacagcag
     1561 aagttgcctt tagccaggga atgggtgtga tatggtatca catcgcctct tcttttggct
     1621 taatcttatt tgggttagct tttgcccatt attatcgcaa atacaatgtt attactgtac
     1681 ctgaatttct ttattatctt tatgacatta aaacatggaa agctgcttca gttttgaatg
     1741 tggtaggcta ctggtttttc accataattc agatgactgc actgggggca ttggtaacaa
     1801 gtgttaccgg tctggattta aagctgagtg tactgatatg tggtatagct atgactattt
     1861 accttttagc agcaggtatg tggtcagttg cctttaccag tgtagctttc atgcttacca
     1921 ttggaactgg gattccaatt gctttctggt gggtaatgaa aaaagaaatt cccctactcg
     1981 ctgcttcggc cggtctttca ggatattccg gtttggccgc tgcactcaac ggaatgggtc
     2041 ttgatgccac cgcacttttc agtccattta gcctgggggg aatggcagtt tttggatatt
     2101 ttattggtgg aatattagga ataccagctg cgcaggccac cattaactat tcttttggtg
     2161 ccaaaaactg gaagatagca cgtttagcac ccgttctggc agctatttta atcttacctt
     2221 taagtatttg gaccggttct atgggccttt ttgctcgtgc agcaggatta accaataatc
     2281 ctaaattagc tctaggggct accttaatga gtattcatcc tatcgttggg ggtattggta
     2341 ttgccggagt ttttgcagca ttgatttcta ctgttgctgg aata
//
LOCUS       DAOQWT010000241         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_50128_length_2378_cov_3.058975, whole genome shotgun sequence.
ACCESSION   DAOQWT010000241 DAOQWT010000000
VERSION     DAOQWT010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50128_length_2378_cov_3.058975"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKJ95_05340"
     CDS             complement(<1..386)
                     /locus_tag="PKJ95_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR65697.1"
                     /translation="MLPEPILKTLELTKSFGNLIAVNNIEIEINRGEIVGLIGPNGAG
                     KTTFFNLVTGLEAPDKGQVVFKGVNIVSMPPHKICKLGMSRTFQITRSFGEMSVEDAI
                     RVGAYNRLGENEVQKKVDEMVEFFEL"
     gene            complement(376..1380)
                     /locus_tag="PKJ95_05345"
     CDS             complement(376..1380)
                     /locus_tag="PKJ95_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR65698.1"
                     /translation="MNFFKEINLGKLLILILFIIALGIPYVLRANQYQVLLATTVLLY
                     GVMATSWNIVGGLAGQLDFAAFAYLGIGAYVSGTLFIRFNITPWFGMILGGLVASGFA
                     LLIGIPLFRFGIKEVWYALTTSALVEILKVAFNMWEKVGGPVERCLPYFSGSWQNMRF
                     VSYAPFYYIMLAMLVMALIVNQKITNSKLGYYLKAIGEDETATEVLGVDNRASKLKAL
                     FYYSFLIGMTGGIYSCMYGYIHPSFFNGPESTKVAILGIVGGRGITYGPLLAALLLVG
                     AQEFLRASLGGEFSGLYLLIYAVIMVIVVLFKPRGIATIFQDIYRKFVVREVKNKDVA
                     "
     gene            complement(1380..2240)
                     /locus_tag="PKJ95_05350"
     CDS             complement(1380..2240)
                     /locus_tag="PKJ95_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR65699.1"
                     /translation="METIIQSVFSGILMGCIYALIALGLSLIFGVMNVVNFAHGNFVM
                     LSMYFTFWAGSLYKIDAIFSPIITFPLLFLFGILVYHGLIKQTLQEHYTIQIAVTVGL
                     MTFLGAITQLVWQARPRALAYSIVQGTIRFGSYTIALSRLISAIISLIIIMIISYFLA
                     KTWPGMAIRATSDDSNAASLMGINFQRTYALAFALGSGLTAIAGGLLMTFQQVDPTMG
                     LRFGLLSFCILALAGLGSITGLVISGLIVGISESLAMTFWDPRARTLVIYLIFILVLW
                     FKPRGIFGRK"
BASE COUNT          813 a          518 c          395 g          652 t
ORIGIN      
        1 tttaattcaa aaaattcaac catctcatca actttttttt gtacttcatt ttcacccagt
       61 cgattgtaag cccctactct tatcgcatct tccacagaca tttcaccaaa agatctggta
      121 atttggaaag tccgggacat acccaatttg caaatcttat gaggaggcat agagactata
      181 ttgacacctt tgaatacaac ctgtcctttg tcaggggctt ccagtccggt tactaaattg
      241 aaaaaagtag ttttaccggc accgtttggc ccaataagcc ctacaatctc tcctctattt
      301 atttctattt cgatattatt tactgcaatt agattaccaa agctcttggt aagctctaag
      361 gtctttaaaa taggttcagg caacatcttt gttctttacc tccctcacta caaatttccg
      421 gtaaatatcc tgaaaaatag ttgcgattcc tcgaggttta aagagaacaa ctataaccat
      481 gataacggca taaatcaaaa gatataatcc cgaaaattcc ccgcccagac tggccctaag
      541 aaattcctgt gcacctacca ataatagggc agctaaaagg ggtccatagg taatacccct
      601 gcctcctaca attcccaata tagctacttt ggtactctcc ggtccgttaa aaaagctggg
      661 atgaatgtag ccatacatac aggaataaat accaccggtc atacctatta aaaaggaata
      721 atagaacaat gcctttaatt tgctggcccg attatcaact cctaaaactt ctgttgcagt
      781 ctcatcctcg cctatagctt tgagataata accaagtttt gagttggtaa ttttttggtt
      841 tacaatcaag gccatcacca gcattgccag cataatataa taaaaaggtg cataagaaac
      901 aaagcgcata ttctgccagg aaccagaaaa ataggggaga catctctcta ccgggccacc
      961 gaccttttcc cacatattaa aagccacttt aagtatttct accagtgcac tggtggtcaa
     1021 agcataccag acttctttta ttccaaagcg gaatagtggg attccgatta ataaggcaaa
     1081 tcctgaagct accaggcctc ctaaaatcat tccaaaccag ggagtaatat taaatcgaat
     1141 aaataaagta ccagaaacat aagctcctat gcccaaataa gcaaaagcgg caaagtcaag
     1201 ctgacctgct aatcctccta caatattcca ggaggtggcc ataactccat ataatagaac
     1261 tgttgtagcc agtaaaacct gatattgatt ggctctaagt acatagggta tacccaaagc
     1321 aatgataaat aaaatcaata tcaatagttt tcctaaattt atttctttga aaaaattcat
     1381 tatttcctcc cgaaaattcc tctaggctta aaccaaagaa ccagaataaa tatgagatat
     1441 attaccagtg ttctggctct tgggtcccaa aaagtcatag ccagagattc gcttattccc
     1501 acaatcaaac ccgaaattac caatccagta atagacccca atccagctaa ggccagaata
     1561 caaaaactga gtaatccgaa tcgtaaaccc atggttgggt ctacctgctg aaaggtcatt
     1621 aataaacccc cggctatagc ggttaagcct gaacccaggg caaaggctaa agcatatgtt
     1681 ctttgaaagt tgattcccat caaagatgct gcattggaat catcagaagt agcccttatt
     1741 gccatccctg gccaggtttt ggctaagaaa taggatataa tcattataat aatgaggctg
     1801 ataatagcag atatcaaccg ggaaagcgca atagtataag aaccaaatcg aatagtgcct
     1861 tgaacaattg aataggccag tgcgcgtggc cttgcctgcc agaccaattg agttatggcg
     1921 cctaaaaagg tcattaaacc cactgttacc gcaatttgta tagtataatg ctcctgaagt
     1981 gtctgtttta ttaatccatg atagaccagt attccaaata aaaataataa agggaaagtt
     2041 ataattggac tgaaaatggc atcaattttg tatagagaac cagcccaaaa ggtaaaatac
     2101 atagaaagca tcacaaaatt accatgggca aaatttacta cattcattac accaaagata
     2161 agtgacaatc ctaaagctat cagcgcataa atacagccca tcaaaattcc actaaaaaca
     2221 ctttgaataa ttgtttccat cttataaagg ctatcctttc ctgtcttcta tgattgcacc
     2281 cgattaatag aaactttgcc tggttaattg tggtattgaa attaaccagg caaagtcctt
     2341 aaagattatt taccggctat agaaaattag taggtagc
//
LOCUS       DAOQWT010000242         2373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_50292_length_2373_cov_3.093615, whole genome shotgun sequence.
ACCESSION   DAOQWT010000242 DAOQWT010000000
VERSION     DAOQWT010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50292_length_2373_cov_3.093615"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /gene="mnmG"
                     /locus_tag="PKJ95_05355"
     CDS             <1..1623
                     /gene="mnmG"
                     /locus_tag="PKJ95_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNR65700.1"
                     /translation="AMWALRAQVDKNRYVREMIKTIEKEPNLTLLQEMVCKLLIKEDT
                     IEGVETQGGMQIFAPAVILTAGTFLDGKIYIGKVCFSAGRAGELASVELANQLKELDF
                     KTTRFNTSTPLRINRRSIDFSELGEQKSADEPLAFSFDSEKKVYNEHSVYIARTNLKT
                     QQVIEKYLKQVSLLSGAVESTSARYCPTIEDKVVRFPHHNTHQLFLEPESVTGEEIYV
                     QGFFTSLPPIAQLEALQTVKGLENCEIVRYGYSIEYDIILPFQLKYSLETKKIKGLFL
                     AGQVNGTSGYEEAAEQGLIAGINAIQLLNKEEPFILDRSEAYIAVAIDDLVTKGVNEP
                     YRLRTGLAEYRLLLRQDNADLRLTPYGYKYSLISKERYEKFLAKKESIEREIKRLNDV
                     KVYPNEDTNKKLEQLNTPSIQKQITLADLITRPGLNYSNTAVLDEERPQLNSEVINQV
                     EIQVKYSGYIARQEEEVRRFKKNENYKIPVDIDYSQVYGISHEGRQRFSEIRPLSLGQ
                     ASRIPGITPSDITALMINLEKLRRTRKDNNHQ"
     gene            complement(1688..>2373)
                     /locus_tag="PKJ95_05360"
     CDS             complement(1688..>2373)
                     /locus_tag="PKJ95_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNR65701.1"
                     /translation="NFDFEKYALSEQTTEEQLLNLLHQLNEDRAIHGIIVQTPLPKPI
                     NEDKIREALSPAKDVDCFNPVNMGKLAMGNPDFLPCTPDAVFEILKREKIEVEGKHVV
                     IVGRSSVVGKPLALILLLKIPNANATVTICHSRTKDLASFTRKGDIVVAAAGSIRLIK
                     GEMIKEGTIVIDVGTNEEEGKLVGDVDFEEVSPLASKITPVPGGVGPITNMMLMKNTL
                     LAAERQLLR"
BASE COUNT          757 a          446 c          488 g          682 t
ORIGIN      
        1 gctatgtggg cattaagggc acaggtggat aagaacagat atgtgagaga gatgattaaa
       61 accatcgaaa aggaaccaaa tctgacactg cttcaggaaa tggtctgtaa gttattaatt
      121 aaagaggata ccattgaggg agttgaaaca cagggaggta tgcaaatttt tgcacctgct
      181 gttattttaa ctgctggtac ctttttagat ggtaagattt atataggaaa agtgtgtttt
      241 agtgccggtc gggcaggaga attagcctct gttgagctgg ctaatcagct caaagaatta
      301 gattttaaga caacgcggtt taatacttct actcctctac gcattaatag aagaagtatt
      361 gatttttctg aattgggtga acagaaaagc gctgatgagc cactcgcctt ctcttttgat
      421 tcggaaaaga aggtttataa tgaacatagt gtctatatcg ctagaactaa cctaaagacc
      481 caacaggtaa ttgaaaaata ccttaagcag gtatctctgc taagtggtgc tgttgaaagt
      541 acttctgcca gatattgccc taccattgag gataaagttg ttcgtttccc acaccataat
      601 acccaccaac tattcctgga accggaaagt gttaccggtg aagagattta tgtacaggga
      661 ttttttacca gccttcctcc tattgcccag ttggaggcat tacaaaccgt taaggggctg
      721 gaaaattgtg aaattgtccg atatggatat tcaatagaat atgatattat cctgcctttt
      781 cagctaaaat attccctgga gactaaaaag attaaaggtt tgtttttagc aggacaggtt
      841 aatggaacat ctggctatga ggaggctgct gaacagggtc tgattgcggg aattaatgcg
      901 attcagctgc taaataaaga agaacctttt attctggatc gctcagaagc ctatattgca
      961 gtagcaatag atgacctggt taccaaaggt gtaaatgaac catatcgcct caggactgga
     1021 ctggccgaat accgcctctt gctgcgacag gataatgctg atttaagatt aactccctat
     1081 ggctataagt atagtttgat ctcaaaggag agatatgaaa aatttttagc aaaaaaagaa
     1141 tctatagaac gggaaataaa aagattaaat gatgtgaagg tatatcccaa tgaggacacc
     1201 aataaaaaac tggaacagct taatactcca tcaattcaaa aacagattac tttagccgat
     1261 ttaatcaccc gccccggttt aaattacagt aatactgcgg ttttagatga ggagaggcca
     1321 caattaaact cagaagtaat caaccaggtg gaaattcaag taaaatactc cggttatatt
     1381 gccagacagg aagaggaagt gagaagattt aaaaagaatg agaattacaa aatccctgtt
     1441 gatattgatt actctcaagt ttacggaatc tctcatgaag gtagacaaag attttcggaa
     1501 ataaggccgt tatccctggg ccaggccagc cggataccag ggataacccc ctctgatatt
     1561 accgccttaa tgattaacct ggaaaaatta agacgaacga ggaaagataa taatcaccaa
     1621 tagatgtgat aattatatta tgaatgatta ttaaaaaagt atttaatgat ttggcttttt
     1681 tttgacacta cctgagcagt tgtctttctg ctgccagcag ggtgtttttc ataagcatca
     1741 tattggttat cggccccact ccaccaggaa cgggagtaat tttggaggca agtggagaaa
     1801 cttcttcaaa atcaacatct cccaccaatt tcccttcctc ttcattggta cccacatcta
     1861 tgactatggt gccctcttta atcatttccc ctttaattaa cctgatagaa cctgcagctg
     1921 ccactacaat atcacccttc cgtgtaaaag aagccagatc tttcgttctg gagtggcaaa
     1981 tagtaactgt ggcattggca ttaggtatct ttaaaagcag gataagagcc aggggtttgc
     2041 ccaccacact acttctacca acaataacta catgcttgcc ttcaacctca atcttctccc
     2101 tttttaagat ctcaaaaact gcatcaggtg tacagggtaa aaaatcggga ttccccatgg
     2161 ccagtttacc catattaacc ggattaaaac aatccacatc cttagcggga gataatgctt
     2221 cgcgtatttt atcctcattg ataggtttgg gaagaggagt ctgaacgatt ataccatgga
     2281 ttgctctgtc ttcatttagt tggtgtaata agttcaataa ttgttcttca gttgtttgtt
     2341 cagatagggc atatttttca aaatcaaagt tga
//
LOCUS       DAOQWT010000243         2373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_50309_length_2373_cov_2.128128, whole genome shotgun sequence.
ACCESSION   DAOQWT010000243 DAOQWT010000000
VERSION     DAOQWT010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50309_length_2373_cov_2.128128"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PKJ95_05365"
     CDS             complement(<1..835)
                     /locus_tag="PKJ95_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNR65702.1"
                     /translation="MRRKGFKINSIIIIAVCSILFFTLISKIIVNSQNESIFNDLEPF
                     LEVLSIIRSEFIDQDVNINKLVPGAIKGMLQELGDPYSRYMDPMSFQREQENLFMGHF
                     HGLGIIVTIVDEQLTVISPIEDTPAYQAGIKANDKILKIDGESTKGINLDEAVNILRG
                     EKGVPVTLTIKRENKEELFEITVIRDTIEVEAVKEKLLEENQVGYIRISTFNANTVPE
                     LKQVLNDFTKLSLEGIIVDLRNNPGGLLESSIEVASQFVKEGDIVKIKSRNQSTKVYH
                     SY"
     gene            complement(891..2144)
                     /locus_tag="PKJ95_05370"
     CDS             complement(891..2144)
                     /locus_tag="PKJ95_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNR65703.1"
                     /translation="MKILGKSYRKRYYKTFSLMLYILFLILIFVEVSITQNKEINIEQ
                     EQDNLNKIEQRKKDIEKEIERLKQEKVDYQKSLQKILELLASAEKELLSTKQSYETTL
                     KEIKKLEEEFEIEQNKLDLQLIILKNRLKKFYKYNNISYFAVLINSKDFSQFLNRYRF
                     LEQILENDADVIKQVNEQVELVKKQRDSLYNKREITKMLEQEIAREKENIEMSLEAKN
                     KYLVKIDEERKKQLVVLEELEKSSAQIKEIIEIAYQEREKAKKAEQKAEERTQTQAKP
                     GTETMKKEPTLKPKKGIFHIPVNGSIISNFGQQKQPDSNALVFNSGIDISAPLGDSVR
                     SASFGTVIYTGNVKGYGDIIILDHGGNVVTLYAHLSKILVKINDQVSKGQIIGQVGTS
                     GGVPSPRLHFEVRVEGKPVNPFEWI"
     gene            complement(2141..>2373)
                     /locus_tag="PKJ95_05375"
     CDS             complement(2141..>2373)
                     /locus_tag="PKJ95_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65704.1"
                     /translation="IEGFLKGFVSSLLAFFSLSKTYQYFIGQVKEIIPFMPIMAGNEF
                     IIRVGIIVLLLGTSIGIFGSMLSLRKISFEEL"
BASE COUNT          701 a          451 c          307 g          914 t
ORIGIN      
        1 cataggaatg gtatactttg gttgattgat ttctactttt tattttcact atatcccctt
       61 ctttaacaaa ttgactggct acttctattg aggattccaa aaggcccccc gggttatttc
      121 tgagatctac tatgataccc tctaatgata atttagtaaa atcatttaaa acctgcttca
      181 attctggtac ggtattggca ttaaaagtag aaattctaat ataaccaacc tggttttctt
      241 ccaacaactt ttctttgact gcttctactt caatagtatc cctgataaca gttatttcaa
      301 acaattcctc tttattttct cttttgatag tgagtgtaac cggtactcct ttttcaccgc
      361 gtaaaatatt aacggcctca tccagattaa ttcctttagt agattcacca tcaattttta
      421 atatcttgtc gtttgcttta attcctgcct gataagcagg ggtatcttct attggtgaga
      481 ttacggttag ttgttcatca actatggtaa ctataattcc taatccatga aaatgaccca
      541 taaataaatt ttcttgctct ctttggaagc tcattggatc catatatctg gaatatggat
      601 cacctagctc ctgcaacatt ccttttatag cacctggcac cagtttgtta atgtttacat
      661 cctggtcaat aaattcactt ctgataatac ttaaaacctc taaaaaaggt tccaggtcat
      721 taaagatact ctcattttga ctatttacaa ttatctttga gataagggta aaaaagagga
      781 tgctacaaac agcaataatt attattgaat ttattttgaa accttttctt ctcataccat
      841 ttaccatcca attctctaaa ttccatccat tatatcacaa taatccattt ttaaatccac
      901 tcaaaggggt tcactggttt tccttctact cttacttcaa agtgcaatct tggcgaaggt
      961 acacccccgc tggtaccaac ctgcccaata atctgtcctt ttgaaacctg atcgtttatt
     1021 tttactaaaa ttttggataa atgggcatat aaggttacca cattaccacc atgatccaga
     1081 attattatat cgccataacc tttaacattc ccagtataaa taacggtgcc gaagctggca
     1141 cttcggactg aatcccctaa aggagcactt atatcaattc cagaattaaa gactaatgca
     1201 tttgaatcag gttgtttttg ttgtccaaaa ttagaaatta tggaaccatt tactgggata
     1261 tgaaatattc cttttttagg ttttaaggtt ggttcttttt tcatagtttc agttcctggt
     1321 ttagcttgag tctgagtcct ttcttcagct ttttgttctg ccttttttgc tttttctctt
     1381 tcctgataag caatctcgat aatctctttt atctgtgctg aagacttttc taattcttcc
     1441 agaacaacaa gttgtttttt tctttcttca tcaattttta ccagatattt attctttgcc
     1501 tctaaggaca tttcaatatt ctctttttct cgtgctatct cctgttctaa catttttgta
     1561 atctctcttt tattgtacaa gctgtccctt tgttttttaa ccagttcaac ctgctcatta
     1621 acctgtttga taacatcagc atcattttct aatatctgtt ctaaaaaacg atatctattt
     1681 aagaattgtg agaaatcctt actattaatt aaaacagcaa aataagatat gttgttatac
     1741 ttataaaatt tttttaacct attttttaaa attattaact gcaaatcaag tttattctgc
     1801 tcaatctcaa attcttcttc cagttttttt atttctttca aggtagtttc ataagactgt
     1861 ttggtagata ataattcttt ctccgcagaa gcaagtagtt ccaggatttt ttgtaaagat
     1921 ttctgataat ctactttctc ctgttttaat cgctctatct ccttttctat atccttcttt
     1981 cgctgttcta ttttattcag gttatcctgt tcttgttcaa tattaatttc tttattttga
     2041 gtaatactaa cttcaacaaa aattaagata aggaagagaa tatatagcat aagggaaaat
     2101 gttttataat atctttttct gtaggatttg ccaagtattt tcataattcc tcaaaactta
     2161 tttttctaag ggagagcatg ctgccaaaaa taccgattga agtacctaat aaaagcacaa
     2221 ttataccaac tctaattata aattcattcc ctgccataat tggcataaag ggaattattt
     2281 ctttaacctg accaataaaa tattgataag ttttacttaa agaaaaaaaa gcaagtagag
     2341 aagagacaaa cccttttaga aatccttcaa taa
//
LOCUS       DAOQWT010000244         2372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_50329_length_2372_cov_3.023738, whole genome shotgun sequence.
ACCESSION   DAOQWT010000244 DAOQWT010000000
VERSION     DAOQWT010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50329_length_2372_cov_3.023738"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="PKJ95_05380"
     CDS             <1..1211
                     /locus_tag="PKJ95_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNR65705.1"
                     /translation="IALSEGQTTIWIDYNESGTTKSDSILVNVYKNPQGMGNLRSINV
                     LPKEMSLDIGESKTISSITAYYDNGTNRIISPYLCNFNIDNPIINISNSGEITGIAEG
                     TSLVTVSYSEEGITRTDTLTVEVGKVAVESPVYRSLSIGIGDYIIYGPEGDLLAPPYD
                     VNKITNMYRDYRFGFNNVPFFKIDTLVEHQATKNNILNKIRNTFFGSKENDISYFYFS
                     GHGALLNQTSYLCPVDFNGNVSTAISVNELESVLSTIPGKKVVLIDSCHSGGFIGKSG
                     SAIEFNMLESNNYLNQFNDNIISAFTDSIISKEPLTSSEYLVLTSSHWFQSSYELNPP
                     NSEPFGVFTQALYEGCSLTYNIAADFNHDDKISLHEAYQFISQWIVSLRINQNVQVSP
                     SNSSFTIFEY"
     gene            complement(1315..2253)
                     /locus_tag="PKJ95_05385"
     CDS             complement(1315..2253)
                     /locus_tag="PKJ95_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65706.1"
                     /translation="MFLKTKKNCSVFFAVIFFLSLILPQIALAQNSSEIAIIEDLSQI
                     EMLIYGEVKEGGLLIRVETLEKELVGRTLPGILKDRVKQLKDFIVTGTPEEPSLSFKI
                     NSAQRTLQDGVSDEPLLPKIEELELIIFGELSDDVLAMRAEKIFTVCFPEGRPSTQEV
                     MVPAGTMIPVVLLQKISSKKSHSGDIFEYKVAEDIAYAGSVIIPKDTIARGEVLKAKK
                     ASVLLKKGELQLDFKTIEAIDGTNIRLVIGEASEEENTRLGVAIGAGIAGLIILSNPI
                     GLAAGALIPGKNVTFKEGTKIYLEVARDTKIISLTR"
BASE COUNT          738 a          433 c          378 g          823 t
ORIGIN      
        1 ttattgccct ttctgaaggt cagactacaa tttggattga ttataatgaa tccggaacga
       61 ctaaaagcga tagtattctg gttaatgttt acaaaaatcc acagggaatg ggaaatctgc
      121 gcagtataaa tgttttacca aaagaaatgt ctcttgatat tggggaatct aagactatat
      181 cttctattac tgcctattat gataatggga caaaccggat aattagtccc tatctttgta
      241 attttaatat tgataatccc ataattaata tcagcaattc aggagaaatc accggcattg
      301 ctgaaggtac ttccttggtc acagtttctt attctgaaga aggaattaca agaactgaca
      361 ccttaaccgt tgaggttggc aaggtagctg ttgagtcccc tgtttaccga tctttatcaa
      421 taggaatagg tgattatata atttatggtc cggaaggaga tttgttagcc ccaccttacg
      481 atgttaataa aattactaat atgtataggg attaccgctt tggattcaat aatgttccat
      541 ttttcaaaat agatacttta gttgaacatc aggcaacaaa gaataatata ttaaataaga
      601 taagaaatac ttttttcggg agtaaggaga atgatatttc ctatttttat tttagtggac
      661 atggtgcttt gttaaatcaa acttcttacc tatgtccggt tgatttcaac ggaaatgtat
      721 ctaccgccat tagcgttaac gaattagaat cagttttaag caccattccg gggaaaaagg
      781 ttgttcttat agattcctgc cattcaggtg gttttatagg caaaagcggt tcagcaatag
      841 agttcaatat gctcgaaagt aataattatt taaatcaatt caatgataat atcatcagtg
      901 cttttactga tagtattatt tcaaaagaac ctctcacctc tagcgaatac ctggtattaa
      961 cttcaagtca ttggtttcaa tctagctatg aactcaatcc acccaatagt gaacccttcg
     1021 gtgttttcac ccaggccttg tatgagggat gttctttaac ttataatatt gcggctgact
     1081 ttaatcatga tgataaaatt tccctgcatg aagcctatca atttatcagt caatggattg
     1141 tttcattacg tattaatcag aatgttcagg tttccccttc caattcctcc tttaccatat
     1201 ttgaatattg atatatcaac tttaagaatt tcttgaagtt gtagttttat tatatttcac
     1261 atataattac attaagataa aatcgtctca ttctatttta aactgaacag ggatttatcg
     1321 tgttaaagag attatcttgg tatctctggc aacttctaaa tatatttttg ttccttcttt
     1381 gaatgttacg tttttaccgg gaattaaagc tccggctgct aaacctatgg gattactaag
     1441 gataatcaat ccagcaatac ctgctccaat ggcaacacca agtctcgtat tttcttcctc
     1501 tgatgcctca ccaataacca atctaatatt ggtaccatca atagcttcaa tagtcttaaa
     1561 atcgagctgt aattctcctt ttttcaataa tacagaggct ttttttgctt tcaacacctc
     1621 acctctggca atagtatctt tcgggataat aacagaaccg gcataggcaa tatcttcagc
     1681 aactttatat tcaaatatat caccgctatg actttttttg gaactaattt tttgtaacaa
     1741 aactactggt atcatggtcc cggcaggaac cattacctcc tgagtgctgg gccttccttc
     1801 agggaagcaa actgtaaata ttttttcagc cctcattgcc agaacatcat ctgaaagctc
     1861 tccaaatatt attaattcca attcctctat tttgggtaaa agcggctcat cgctaacccc
     1921 atcctgtaaa gttcgctggg ctgaatttat tttaaaggat aatgatggtt cttccggtgt
     1981 acctgtaaca ataaaatctt ttaactgctt taccctgtct ttcaaaatac ctggaagcgt
     2041 tctacctacc agttcttttt ccagtgtctc taccctgata agtaagccac cttctttaac
     2101 ttccccatag attagcattt caatctgact caaatcctca ataatagcta tttctgatga
     2161 gttttgagcc aaagcaattt gtggtaagat aagggataaa aaaaatataa cagcaaaaaa
     2221 aacagaacaa ttcttcttag tttttaaaaa catataattt cacctcaata attaaagttt
     2281 ttggtttatg gtttttggtt ttagaattaa attaagatgt actattttca agttgtgttt
     2341 acactttaga ttaaattagc ttaaaaaaat tg
//
LOCUS       DAOQWT010000245         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_50446_length_2369_cov_2.625324, whole genome shotgun sequence.
ACCESSION   DAOQWT010000245 DAOQWT010000000
VERSION     DAOQWT010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50446_length_2369_cov_2.625324"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1224)
                     /locus_tag="PKJ95_05390"
     CDS             complement(172..1224)
                     /locus_tag="PKJ95_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65707.1"
                     /translation="MDKYLLILWFILCFILTGCIKEEKYIATPTVQYMITPQIKDYIS
                     TGEPNIKKSPYAPFTLDPEGNFYIADDTIDSRMNIEKYNSQGNYITGWIPEIKKRKKN
                     CWFLERKHPISEQLTDILADKENIYIAYGAAYNINFKEGNFPSDCISHIEQFDLNGKF
                     IIAIKPAYDSFHTYFIRYITKDKNGNFYLASDIHVEKYNANWKLQSILNPFDPEKSKL
                     QPGVLIGDVAADSQGYIYILLTSPDYYENGYVAKFDSFGKLVNKWNSEDKDKLIFCGL
                     EIDADDNIYLLGIDRKDTYHIQKFYSNGIFITEWNTECTGELCFNPYNDMRIDCKGNI
                     YVASVKCTIKKFSPKQ"
     gene            complement(1229..>2369)
                     /locus_tag="PKJ95_05395"
     CDS             complement(1229..>2369)
                     /locus_tag="PKJ95_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65708.1"
                     /translation="STSDPIIIKNEDTGEPLPEKEEIKHLSCLTASSNNTTVKNSDVI
                     ILSPYYWQQPSTLQVERKIPQALKDNNYNVKEIRPVEGRDFVLNWDTGVEPWVDCVVK
                     SSWDNILTPEALETMGKYGIIFINAHGAPVNIDENSFDPYNVPPGTVFRMSCTPLFRN
                     TNPHDVTPIFRWVIEHIEKSYIPFDDKRSKGWWFFCVREIEEEVQIGKWKKGWILDLD
                     LTNYYLDSLNASSATNFDGSLVYWNSCYSWHMKNSFNTAKAFIGLDRPSIANWSYPFG
                     YYFFYFMMYGNKNCPKDQNGKILIPSAVLPEGTPSFDPNAPLHAEEALNALTNYYKVN
                     PDPTIYSEIEQLFCLACKVKIWQKEPNEHIYFPVPVTVTIEKKGD"
BASE COUNT          741 a          421 c          372 g          835 t
ORIGIN      
        1 gtatgcaatg tctcctgaac ctgtatctgt ataatccggt gcaggggtaa tcagagaaga
       61 cggctgagta gtttcacttc ctcctccaca gccggaaatt atggctatgg caagcagggt
      121 tattattaat agtgttaata aaagtttaac ttgcattacg agtccccttt cttattgttt
      181 tggtgaaaat ttcttgattg tacattttac agaagcaaca taaatatttc ctttacaatc
      241 tattctcata tcattatatg gattgaaaca tagttctcca gtgcattcag tattccattc
      301 ggttataaaa atcccattgg aataaaattt ctggatatga taggtatctt ttctatctat
      361 acccagtaaa tatatgttat cgtcagcatc tatttctaaa ccacaaaaaa tcaatttatc
      421 tttatcttca gagttccatt tatttactaa tttaccgaaa gagtcaaatt tagcaacata
      481 gccattttca taatagtcag gcgacgttaa taagatataa atatatccct gagaatctgc
      541 tgctacatca cctattaata caccaggttg taatttagac ttttcagggt caaatggatt
      601 taaaatcgat tgtaatttcc aattagcatt atatttttct acatggatat ctgaggctaa
      661 ataaaaatta ccatttttat cctttgtaat atatctgata aagtatgtgt gaaaagaatc
      721 gtatgccggc tttatggcaa ttataaattt cccattgaga tcaaattgct caatatggct
      781 gatacaatca cttggaaaat ttccttcttt aaaattgatg ttatatgctg caccgtaagc
      841 aatataaata ttttctttat cggcaagaat atcagtgagt tgttcgctta taggatgctt
      901 tctctctaaa aaccagcagt tcttttttct cttttttatt tcaggaatcc agccggttat
      961 atagttcccc tgtgaattat atttttctat attcatacga gaatctatcg tatcgtctgc
     1021 tatataaaaa ttcccttcag gatccagtgt aaatggagca tatggagact tcttaatatt
     1081 gggctcacct gtagagatat agtctttaat ttgaggagta atcatatatt gtactgtagg
     1141 agtagctata tatttctcct cttttataca acctgtaagg ataaaacaca atataaacca
     1201 taatattaat aaatatttat ccaccacgtt aatcaccttt tttctcaatc gttacagtta
     1261 caggaacagg aaaataaata tgttcattcg gctctttctg ccatatttta actttacagg
     1321 ctaaacaaaa tagttgctca atctctgaat atattgtcgg gtcaggatta actttgtagt
     1381 aatttgttaa tgcatttagg gcctcttcag catgcaatgg tgcatttggg tcaaaagatg
     1441 gtgttccttc aggtaaaact gcactaggta ttaatatctt tccgttttga tctttaggac
     1501 aatttttatt accatacatc ataaagtaga aaaaataata tccaaaaggg taactccaat
     1561 tagctataga aggtctatct aaacctataa atgctttggc agtattaaaa gaatttttca
     1621 tatgccagga ataacaacta ttccaataga caagtgaacc atcaaaattt gtcgcagaac
     1681 tggcgttaag ggaatctaaa tagtaattgg taaggtcaag gtctaaaatc catccttttt
     1741 tccacttccc aatctgaact tcttcttcta tttctctgac acaaaaaaac caccatcctt
     1801 tacttctttt atcgtcaaaa ggaatatacg atttctcaat atgctcaatt acccacctga
     1861 atattggtgt aacatcgtga ggattggtat tccgaaacaa aggagtgcag gacattctaa
     1921 aaactgttcc tggaggtaca ttatatggat cgaagctatt ttcatctata tttacagggg
     1981 ccccatgagc attaataaaa attattccat actttcccat agtttcaagt gcctcgggag
     2041 ttagaatatt atcccaggat gattttacaa cacagtcaac ccatggctcc acgccagtat
     2101 cccagttgag aacaaaatct ctaccttcta ctggtcttat ttcttttaca ttataattgt
     2161 tatcttttaa tgcttgtgga atttttcttt ctacttgcaa cgttgaaggt tgttgccaat
     2221 aataaggaga tagtattatc acatcagagt ttttaacagt ggtgttatta gaactggcag
     2281 taaggcaaga aagatgtttt atttcctctt tctcaggtaa tggctcgcct gtatcctcat
     2341 tctttattat tattggatct gaagtggaa
//
LOCUS       DAOQWT010000246         2351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_51049_length_2351_cov_3.143293, whole genome shotgun sequence.
ACCESSION   DAOQWT010000246 DAOQWT010000000
VERSION     DAOQWT010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51049_length_2351_cov_3.143293"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKJ95_05400"
     CDS             <1..424
                     /locus_tag="PKJ95_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNR65709.1"
                     /translation="HTVDRNSISDMVERGIKLHGHSGPYLNLGIKMGLLALNLLEVKG
                     YFDLSTEVEMEYRTPKSCLIDGLQISTGCTMGKGNIRVIDNPETIKVLFKSDKKTLQV
                     TLKPEINKLINFEKVSCEDLAEQILTMPDKQLFDYKFN"
     gene            709..1680
                     /locus_tag="PKJ95_05405"
     CDS             709..1680
                     /locus_tag="PKJ95_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNR65710.1"
                     /translation="MNLQTKMWILVIAMFAILYGVIAGIGSYLGAGSVTSYIILAIGV
                     TLFQYLIGPNMVTSMMKVRWVEVSEEPELHQMIEKMSREAGIPKPKIGISQMAVPNAF
                     AFGRSIQDSRVCITTGIQKLLNKDELKAVIGHELSHIKHRDMIVITVLSVIPLILYWL
                     GMRTMYGGASRNQEKKGSSALIGMLALILYFISNLLVLYGSRIREYYADQGSVSLGNP
                     PHQLATALYKLTYGSAKLKESQMGRQALSQVEGFRAFFLNDVARGYQEFSTLKELDQD
                     FSGTIDAQELFTLREKTIRISTSEKMMELFTTHPNMLKRIKALAKLS"
     gene            2043..>2351
                     /locus_tag="PKJ95_05410"
     CDS             2043..>2351
                     /locus_tag="PKJ95_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR65711.1"
                     /translation="MFVKNKGLNVNLTVKTLIYIFIGMMLLNVSAFSADLPGKIIVGC
                     LEPLTGAHAVFGIEGKVGMEFAVKHINEAGGIKSLGGIPLELVIEDVGEDAMSARLAT
                     E"
BASE COUNT          807 a          372 c          451 g          721 t
ORIGIN      
        1 acatactgtt gaccgcaata gtatcagtga tatggtagaa cgtggtatta aactacatgg
       61 tcattcggga ccttatctta atctgggaat taagatggga cttcttgcct tgaatttgct
      121 ggaagtaaag gggtattttg atttatcaac agaagtagaa atggaatatc gtaccccaaa
      181 gtcttgctta attgatggtt tacagatatc taccggctgt accatgggaa aaggtaatat
      241 aagggtaatt gataaccctg agaccatcaa agttcttttt aaatctgata agaaaactct
      301 tcaggtcacc ctaaaaccgg aaataaataa attaattaat tttgaaaaag taagctgtga
      361 ggatcttgca gaacaaatct tgactatgcc tgataaacag ctctttgatt ataaatttaa
      421 ttaaaaacaa aaaagactta cttttctggt ttataaaatg aataagataa aataagcctt
      481 ttgttttgtt atatattcta acacactata cacactgcac caaaatttct ggctgtactt
      541 cattgtgacc taaaaaagca ttctagaatt aaccttttct acctacgcct caattgatta
      601 ttattattat ttatgattat tacttgagga tatagttaga atacattata attataatat
      661 atctgcattt ctgatatatc aaatatgaaa attaaaagga gatttggcat gaatcttcaa
      721 acaaaaatgt ggattttagt aattgcgatg tttgccattc tttacggtgt tattgcaggt
      781 ataggtagct atctgggagc aggaagtgtt actagttata ttatattagc cataggtgta
      841 acactgttcc aatacttaat cggccctaac atggttactt ctatgatgaa agtaagatgg
      901 gtagaggtat cagaagaacc ggaacttcat cagatgatag aaaaaatgtc ccgggaagcc
      961 ggtataccta aacccaaaat aggtatttcg cagatggcgg tccccaatgc ctttgccttt
     1021 ggacgaagta ttcaagatag cagagtctgt attaccaccg ggattcaaaa actgttaaat
     1081 aaagatgagt taaaagcggt aataggacat gaattatccc acatcaaaca cagggatatg
     1141 attgttatca ccgtattgtc ggtaatacca ttaatccttt actggctggg aatgcgcact
     1201 atgtatggag gtgcctcaag gaatcaagaa aagaaaggct catctgcact tattggcatg
     1261 cttgccttaa ttttatattt tatctctaat cttttagttc tttacggttc ccgtatcaga
     1321 gaatattatg ccgaccaggg aagtgtcagc ttgggaaacc ctccacatca attagctacc
     1381 gctctttaca aattaactta tggttctgcc aagctcaaag agagccagat ggggcgacag
     1441 gcactcagtc aggttgaggg gttcagggca ttttttctca atgatgtggc cagaggttat
     1501 caggaatttt caaccttgaa ggaactggac caggatttca gcggtaccat cgatgctcag
     1561 gaactgttta ccttaagaga aaaaacaata agaatcagta catctgaaaa gatgatggaa
     1621 ttgttcacca cacaccccaa catgttaaag agaattaagg ctttagctaa attatcctga
     1681 aaaacatcca aaagatagta acgtagtgtc ctttttattt aaaataatta gattctaaaa
     1741 tttttaatag ttatgtggat aatttgttga tctattaata accagataaa agtcatagaa
     1801 aagtttagta caaatgaaga acaatagtaa taacaagaat aattccaata ttaactatga
     1861 caaacaatcc attacacctt cccttgtcta tatatccgaa aactaattac ggagaaattg
     1921 tattattgta ttgctagaac atagaagcag aaaaaagaat acttaaaaaa ggagggatga
     1981 aaaagatttt aacatctggc taatttgata cttataaatt ttaattaaaa aggagaagta
     2041 ttatgtttgt aaaaaataaa ggcctgaatg taaaccttac ggtaaagact ttaatctata
     2101 tttttatagg aatgatgtta ttaaatgtct ctgctttctc agctgatttg ccgggaaaaa
     2161 ttatcgttgg atgccttgaa ccccttaccg gtgcacatgc agtcttcgga attgaaggaa
     2221 aagttggtat ggaatttgca gtaaagcata tcaatgaggc tggtggtata aagtctttag
     2281 gtggtatccc attagaatta gtgattgaag atgtgggtga ggatgccatg agtgcacggc
     2341 tcgccacaga g
//
LOCUS       DAOQWT010000247         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_51085_length_2350_cov_2.718954, whole genome shotgun sequence.
ACCESSION   DAOQWT010000247 DAOQWT010000000
VERSION     DAOQWT010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51085_length_2350_cov_2.718954"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKJ95_05415"
     CDS             <1..846
                     /locus_tag="PKJ95_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR65712.1"
                     /translation="ACIMAAVPIAVKAGLFSMVSSTSYEYAVEYFPWTLSWYPITEEK
                     LPPITAAWAKLHPDMKTVVQFVENWAAWPPMAEAHAVGLESAGVKMLSQVDVPTDAIM
                     FEPLVLKALAQKPDGIMLTCNSEKAAKIIIELVKKGWTDKSKILVFSSADDTALYTTG
                     GDSLEGISIYNYIDLNLDDPRWQAFREAYKKDHNGIEPASLSTNYYDSVYMIKEAIEN
                     TGVTGDPAKLAEERIKIRDYCRNVKGFKGIQLTWDMTDGVPTGKGTFLFEIENGEKVF
                     KAMAK"
     gene            986..2212
                     /locus_tag="PKJ95_05420"
     CDS             986..2212
                     /locus_tag="PKJ95_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNR65713.1"
                     /translation="MSKYDNIVNNIDQKGVIQFTQDLVRIKSVFHTKKEGFNEEKVAH
                     FVANKLKSMGIEVFIDEVVPGRPNVIGIIKGKRDGRRLLFEAHTDVVTEGNPEEWKYP
                     PFEAIIHEGKIYGRGACDTKGNLAAMIYAVKAIIDAKAEFKGEIILCIPVDEEGMMLG
                     IKHFIKQGWADKVDAAIICEPEENNICIYQKGALRVKIVVKGKMAHGAMPEAGINPNW
                     GMAKILLELEKLQQKEIKRLGKHKFLGYPSFTPTILKAPVQGEAQINVIPQECMSTID
                     IRTVPGQNHEIIKKSIINIFKKLSKKDGNFQADMEVLEERPWTETSPEEPIVKAISEA
                     YRYISKKEPIYNGVPGATDGTFLNSWKGIPIVTIGAGNRLIPHQINEYVEIEELVETV
                     KIYILSAMLFLNEEVQ"
BASE COUNT          852 a          352 c          486 g          660 t
ORIGIN      
        1 gcttgtatta tggcagcagt acccattgca gtaaaggctg gattatttag tatggtttct
       61 tcaaccagct atgaatatgc agttgaatac ttcccctgga cactttcatg gtatccaata
      121 actgaagaaa agcttccacc catcactgct gcctgggcaa aattacatcc agatatgaaa
      181 acagtagtac agtttgttga aaactgggct gcctggccac ccatggcgga agcacatgcc
      241 gttggtctgg aatcagcagg agtgaaaatg ttatctcagg ttgatgttcc aactgatgcc
      301 ataatgtttg aacctcttgt attaaaggct ttagctcaga aacctgatgg cattatgtta
      361 acctgtaatt cagaaaaagc agcaaaaatt attattgaat tggttaaaaa gggttggacc
      421 gataaatcaa aaatattagt cttcagtagt gcagatgata ctgctttgta caccactggt
      481 ggtgatagtt tagaagggat ttcaatttat aactatattg acctgaactt agatgatcca
      541 cgctggcagg cattcagaga agcttacaaa aaagaccaca atgggataga accagcttct
      601 ctttccacca attattatga tagtgtttat atgattaaag aagcaattga aaacaccgga
      661 gtaacaggtg atcccgcaaa attggccgag gaaaggatta aaataagaga ctattgccga
      721 aatgtcaaag gcttcaaagg aattcaactt acctgggata tgactgatgg agtgcctacc
      781 ggtaaaggaa ctttcttgtt tgaaatagag aatggagaga aagtatttaa agcaatggca
      841 aaataggatt gattgctaaa tagtgaaaca aaaaaataaa atatcagtta tttaaccaat
      901 ccagcccctc ttcttaattt tatatttaaa ataagtaaga ggggctgaag aaaataaata
      961 aggtgtataa ataaaagaat tgtctatttc aaaatatgat aatattgtca acaatattga
     1021 ccaaaaggga gttattcaat ttactcagga tttggttaga attaaaagtg tatttcatac
     1081 taaaaaagaa ggattcaatg aagaaaaggt agcccatttt gttgctaaca aattaaaaag
     1141 tatgggtata gaggttttta ttgatgaagt agtgcctggt aggcctaatg taataggaat
     1201 tattaaggga aaacgagatg gaagaagatt gctttttgag gcgcataccg atgttgtaac
     1261 tgaaggtaac ccggaagaat ggaaataccc accatttgaa gctatcatcc atgaaggtaa
     1321 gatttatgga aggggagcat gtgataccaa aggtaattta gcagctatga tatacgcagt
     1381 taaagctatt atagatgcaa aagctgaatt taaaggtgaa atcatattat gtattcccgt
     1441 tgatgaagaa ggcatgatgt taggtataaa acattttatt aaacaggggt gggctgataa
     1501 agttgatgca gcaattattt gtgaacccga agaaaacaat atctgtattt atcagaaagg
     1561 tgcgctaaga gtaaagatag tggtaaaagg taaaatggct catggtgcta tgcctgaagc
     1621 tggaatcaat cccaactggg gtatggcaaa aatattgtta gaattagaaa agctacagca
     1681 gaaagagatt aaaagattag gcaaacacaa gtttttgggg taccctagtt ttaccccaac
     1741 aatactgaaa gcaccggttc aaggtgaggc acaaatcaat gttattccac aggaatgtat
     1801 gtcaaccata gatatcagga ctgttcctgg acaaaatcat gaaattataa aaaaatctat
     1861 tataaatatt tttaaaaaac tctctaaaaa agatggtaac ttccaggctg atatggaagt
     1921 tcttgaggaa cgaccctgga cagaaacttc gcccgaagag cctattgtca aagctatatc
     1981 tgaagcatat agatatataa gcaaaaagga acccatttat aatggagttc ctggagcaac
     2041 tgatggaact ttcttgaatt catggaaagg tattcccatt gtaaccatcg gtgctggtaa
     2101 tagactgata cctcatcaga tcaatgaata tgttgaaatt gaagaattag ttgaaacagt
     2161 aaaaatttac atcctatcag ctatgttatt tctcaatgaa gaagttcagt aacacgcttt
     2221 tttaattatt aaatatggta ataaaagagg tgaaatacca cgaaatcttc taatcattca
     2281 caaatattaa agaattctat tattgatatt tccgggtgaa agtgggtcac agtcaagatt
     2341 taaaaggcgg
//
LOCUS       DAOQWT010000248         2348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_51143_length_2348_cov_4.294810, whole genome shotgun sequence.
ACCESSION   DAOQWT010000248 DAOQWT010000000
VERSION     DAOQWT010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51143_length_2348_cov_4.294810"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(607..1350)
                     /locus_tag="PKJ95_05425"
     CDS             complement(607..1350)
                     /locus_tag="PKJ95_05425"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNR65714.1"
                     /translation="MDVVKEYYNKINGIMQTIINQERENIEKAALLVAQTVLNDKLFY
                     VFGTGAHSIMSAMEVFKRAGGLCNAQGIFPPGLTDFNGTPKTERVNNFASEIFAWYGV
                     EKNDLLLLCNVNGINPMTIDAALEAKKIGIKTIGVTSIEFAENAEHNIPNRHPSNKNL
                     HELVDLVIDAHVPVGDALLDIPGVPVKVGAGSTYPMILIVNSIIVRAISIVAEKGGEP
                     PVLLSGNIAQGEPYNEKYYKKYRMRIRHF"
     gene            complement(1534..>2348)
                     /locus_tag="PKJ95_05430"
     CDS             complement(1534..>2348)
                     /locus_tag="PKJ95_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HNR65715.1"
                     /translation="PDIELIIYNYIRDYPTHGPRRISNELKAQGITISETGIYNVLRR
                     GGLNHRLDRLFYAQEHSDNPVITEHYLREVEKRKEAHIEAHYPGYLLCQDTFYVGTIK
                     GLGRIYQQAGIDVYSNFSFAKVYFDKKAGSAIDFVKSKVLPVYGIFGIPLDRILTDNG
                     KEYTTHWKSRKHDYEIFLAKRGITHTRIKPRCPRSNGMVERFNRTLLEEFYQIAMLKR
                     VYTTIDELQHDLDKFIYYYNFKRTNQGYRLKGKIPYQKFLDGKRKFSLPEPK"
BASE COUNT          711 a          433 c          345 g          859 t
ORIGIN      
        1 atcatcaata attgattctt ctttctgttg gagtgaatac atgattttta gcaactgttt
       61 aattaccgct agaaacaaag gttatctcaa atattgcctc ttaattgttt ctagccacat
      121 ctccattccc cgcaagaatt gtttatatct ttatcattaa aatattttta tattactaat
      181 tcttatcaat aatattgatt acttcctttt tagaattttt ataatagatt ctaattattt
      241 ctattattat ttttttctta ttccataatg aaataaagga acctcccgcc aagtccttta
      301 taactgtaga aaatcaataa aaatagtgtg tatttcacta taccaaagct tatatctatt
      361 ctttaggtct atagcattta ttcttatacc catagaatag tttataaaaa agttatcctt
      421 gcttcaagta ttttccaaag tacattctaa tgaaaaatac catgtcttta attttttata
      481 aattaattaa caaaaatgac tttcacactc tcttttaacc tcgaaggctg taaacaataa
      541 ttttgttttg gttaatgatt ataaagagga ttatcgttaa agttttaaat tatttaaatg
      601 cttatcttaa aaatgccgta ttctcattct atatttttta taatatttct cattataggg
      661 ttcaccttgt gcaatatttc cagaaagtaa aacagggggc tctccacctt tttcagcaac
      721 tatactaatt gctctaacaa taatggaatt gactattaaa atcattgggt aagttgaacc
      781 agcgccaact ttaactggca ccccgggtat atcaagcaag gcatccccta caggtacatg
      841 ggcatcaata acaaggtcaa ccagctcatg taaattttta ttagatggat gtctatttgg
      901 gatattatgt tcggcattct cagcaaattc aatggatgtt actcctatag ttttaatccc
      961 tatttttttt gcttcaagtg ccgcatcaat tgtcattgga tttataccat taacattaca
     1021 aagtaacaat aaatcatttt tttcaacacc ataccaagca aatatttctg aggcaaaatt
     1081 gttaactctt tcagttttag gagtaccgtt aaaatctgtt aagccaggcg gaaatatgcc
     1141 ttgagcatta catagcccac ctgctctttt aaaaacttcc attgcagaca ttatagaatg
     1201 tgcacctgtt ccaaaaacat aaaaaagttt gtcgttcaaa accgtttgag ctaccagcaa
     1261 ggcagctttt tcaatatttt cacgttcttg attaataata gtttgcatta tcccattaat
     1321 tttattatag tactctttaa caacgtccac ttgtatatat tacctcctta atataatatt
     1381 atgtatttaa aactaaataa acaaactata caatttattt tgatagagtc tataagtaag
     1441 tgataaaata aattcctaat agttatttta aatgtattac tttcatgatg tatttacaca
     1501 ttgccattct ttcctataga aagatgatat gatttatttt ggttccggta aagagaattt
     1561 tcgtttccca tctaagaatt tctgataggg aatcttgcct ttcagccggt aaccctgatt
     1621 cgttctttta aaattgtaat agtagataaa cttatccaga tcatgttgca attcatctat
     1681 tgtagtgtat accctcttaa gcatggcaat ctggtagaat tcttctaaaa gtgttctgtt
     1741 aaatctttct accatgccgt tacttctcgg acatctgggc ttaattctgg tatgggtgat
     1801 accacgcttg gccaggaata tctcataatc atgcttcctg cttttccagt gtgtagtata
     1861 ctctttgcca ttgtctgtta ggatgcgatc cagaggaata ccaaagatac catatactgg
     1921 taataccttg gattttacaa aatcaatagc gctgcctgcc ttcttatcaa agtacacctt
     1981 ggcgaagctg aagttgctgt atacatcaat accagcctgc tgatagattc tacccagtcc
     2041 cttgatggtg cctacataga aggtatcctg gcataagaga tacccgggat agtgtgcttc
     2101 aatatgggcc tcttttctct tctctacctc tctgagatag tgttcggtga taaccggatt
     2161 atcagaatgt tcctgggcat agaagagtct atccagacgg tgattgagtc cgcctcttct
     2221 taagacatta tagatgccag tctcacttat tgtgatacct tgtgccttta actcattgga
     2281 tattcttctt ggcccatgtg ttggataatc tcgtatgtaa ttgtaaatga tcagctctat
     2341 atctggtc
//
LOCUS       DAOQWT010000249         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_51196_length_2347_cov_3.030977, whole genome shotgun sequence.
ACCESSION   DAOQWT010000249 DAOQWT010000000
VERSION     DAOQWT010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51196_length_2347_cov_3.030977"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PKJ95_05435"
     CDS             <1..676
                     /locus_tag="PKJ95_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNR65716.1"
                     /translation="DILRNELQTKWPEFRLASFHTGSMGGLLALKQGIAHLATSHLLD
                     VESGEYNFPYLERILAGHPLVVVNMAYRQQGFMVAKGNPKKILEIKDLARDDVRFVNR
                     QKGSGTRVLLDYLLKDADINSSAIQGYNQEEFTHLMVASAVANSRVDAGLGIFSAPSA
                     FGLDFIPLIKERYDLIIPERYFDSQRITKILEIMKTNYFKEQVANLGGYDLSECGKVL
                     LNGKTH"
     gene            660..1670
                     /gene="moaA"
                     /locus_tag="PKJ95_05440"
     CDS             660..1670
                     /gene="moaA"
                     /locus_tag="PKJ95_05440"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNR65717.1"
                     /translation="MEKLIDSYGRQIVYLRISLTDRCNFRCIYCSPSECKFRFIEHEQ
                     ILRFEEILEIANAAVEMGITKIRLTGGEPLVRKGVVGFIRQLRQIGGLEDISMTTNGF
                     FLSEMAQSLKSSGLDRVNISLDSLVREKFRAITGLDELPRVLEGIDAAFREGLTPVKI
                     NVVLLKGINDDEVESFIKLTIARPLNVRFIELMPTNQVLKEITADHFISLQKIQEKIQ
                     GKFPDISPASVEKGCGPASYYQLKGAKGIFGFITAVSQHFCARCNRVRLTAEGNLRPC
                     LYSFKELELKKELRKIPLNEHKLRNKLIKSFLKEAVKAKPLRHGLTEKNSAEFDMSKI
                     GG"
     gene            1686..2162
                     /gene="moaC"
                     /locus_tag="PKJ95_05445"
                     /pseudo
     CDS             1686..2162
                     /gene="moaC"
                     /locus_tag="PKJ95_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
BASE COUNT          767 a          357 c          560 g          663 t
ORIGIN      
        1 ggatatttta aggaatgaac tgcaaaccaa atggccggaa tttcgcctgg catcatttca
       61 tactggaagt atgggcggac ttctggcttt aaagcagggg attgctcatc tggcaacatc
      121 tcatctttta gatgtagaaa gtggagaata taatttcccc taccttgaac gtattctagc
      181 aggacatcct ttagtggtgg taaatatggc atacagacaa caggggttta tggtagcaaa
      241 aggaaatcct aagaaaattt tagaaataaa agatttagca agagatgatg tccgttttgt
      301 caatcggcaa aaaggctctg gaacaagggt tcttctagat tatttactta aagatgctga
      361 tattaattct tctgctattc aagggtataa ccaggaagaa tttactcatc tcatggtggc
      421 ttctgcggtt gccaacagta gagtcgatgc agggttggga atattttcag ctcccagtgc
      481 gtttggcctg gacttcatac ctcttattaa ggaacgttat gatttaatta taccggaaag
      541 atattttgat agtcagagaa taacaaaaat attagaaata atgaaaacaa attattttaa
      601 ggagcaggtt gccaaccttg gtggttatga cttgagtgag tgtggcaaag tattgttaaa
      661 tggaaaaact cattgattca tatggccgac aaatagtcta tcttagaatt tccctcactg
      721 accgctgtaa ctttcgctgc atctattgct caccctcaga gtgtaaattt cgtttcattg
      781 aacatgaaca aattctacgt tttgaagaga ttttggaaat agcaaatgca gcggtggaaa
      841 tgggtataac taaaatcagg ctgaccgggg gagagccttt ggtaagaaaa ggcgtggtag
      901 gttttataag acaactaaga cagataggtg ggctggaaga catttctatg actaccaatg
      961 gcttttttct ttcagaaatg gcacaatctt taaaaagttc cggtctggac agggttaata
     1021 tcagtcttga ctcactggtt agagaaaaat tcagggcgat tactggtttg gacgaattgc
     1081 caagagtact ggaaggaata gatgctgcct ttcgggaagg tttaacgccg gttaaaatta
     1141 atgtggtact gttaaaaggc attaatgatg atgaggtaga aagctttatt aaactcacta
     1201 ttgcccggcc attaaatgtt cgttttattg aattgatgcc cactaaccag gtattaaaag
     1261 aaattactgc agaccatttt atatcgttac aaaaaattca ggaaaaaatt cagggaaaat
     1321 tcccggatat aagcccagct tccgtagaaa aaggttgtgg ccctgcctct tattatcaat
     1381 tgaaaggtgc aaaaggaatt tttgggttta taactgcagt gagtcagcat ttttgtgcca
     1441 gatgtaatcg tgtcagattg accgcagagg gtaatttaag accctgcctc tatagtttta
     1501 aagagctgga gttaaaaaaa gagttgagaa agataccgct aaatgaacat aaactgagga
     1561 ataaattgat aaagagtttt ctgaaagagg cggtaaaggc aaaaccgtta cgtcacggac
     1621 tgacagaaaa aaatagtgca gaatttgata tgtctaagat aggaggttag aggaggtatc
     1681 aaaatatgga taatggagaa atattaagcc atttaaatga aaaaggaagg gctcgtatgg
     1741 ttaatgtggg agagaaacag gatacccaca ggattgctat tgctgaaggc gaagtaatta
     1801 tgaaaccgaa aactctgact ttaattaaag ataaaaagat ggctaaggga gatgtgctgg
     1861 gtattgccca gatagctgga attatggcgg ccaagcagac tagtcaggct attcctctct
     1921 gtcaccctct tattctgacc aatgtagata tggattttta ttttatggaa gaaaggaaca
     1981 gcatcttaat tcgttccatt gtcatgacta ccggtaaaac gggagtggag atggaagcat
     2041 tgcatgctgt gacaattgct gctcttacca tttatgatat atgtaaagcg atagaccggg
     2101 gtattgaaat aagtaatatt catctggtag aaaaagatgg aggacgtagc ggtcattatc
     2161 agggaaaatc atatcttaat ttagagcgtg ataaaggact agatagtcag agtaaatcag
     2221 ctgataggga aattaccggg aaaggagttg tgctggcgat ttgtgtgggt gaaagcagac
     2281 agagccccaa gaggcaggta acgagtgctt ctgtgattga agattttggt ttagaaggcg
     2341 atataca
//
LOCUS       DAOQWT010000250         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_51267_length_2345_cov_2.450218, whole genome shotgun sequence.
ACCESSION   DAOQWT010000250 DAOQWT010000000
VERSION     DAOQWT010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51267_length_2345_cov_2.450218"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1586
                     /gene="murC"
                     /locus_tag="PKJ95_05450"
     CDS             177..1586
                     /gene="murC"
                     /locus_tag="PKJ95_05450"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNR65718.1"
                     /translation="MNNSGKRIHFIGIGGAGMSGIASLMLELGYQISGSDLNSNISTE
                     RLIDKGALIYKGHDGRHVLGANLVVISSAINKENQEVIAARKNNIEIIPRAEMLSRLM
                     NQKYRIAVSGTHGKTTTTSMISLLLEGMNLDPTIAIGGEVNDIGGNAKLGRGDYVVVE
                     ADESDGSFLLLNPNIAIITNIENDHLDYFTNLEKTMESFQQFIEKIPETGSLLYGYDC
                     PNTRMVAERVAGKKNVISYGFHQNAKVRAANTCLSKKTSLSEIYFGQHKLGNLKLNIP
                     GYYNVSNALAAIATCLQLGLKFQDMAEILYHFNGVKRRQEIIFRLNDEFMIMDDYGHH
                     PSEIKATLQAIRLGWPECRIITIFQPHRYSRTKLLLDYFGTAFQDSHIVIVNEIYAAN
                     EKPIPNISAELIYQKIKKEQGENIYYLPGREDTVSFLRGIIAPGDIVITMGAGDVWKV
                     GTELAKKIKGFNKNRGIDC"
     gene            1586..>2345
                     /locus_tag="PKJ95_05455"
     CDS             1586..>2345
                     /locus_tag="PKJ95_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNR65719.1"
                     /translation="MYNKNQIWREIISNGLEKGISCNEPMKKYTSWKIGGNADFFCTP
                     SHQEQLAKLLLFALEHSLPIYIIGNGTNIWVSDEDIRGLVVRIAHTLDKVEYSDKMIK
                     TGAGILLPALAKKAADRGLSGLEFAAHIPGTLGGAILNNASFGKESISDIVSEITLLH
                     YRKGNIVTLKRGQFSFFYREIDLGYNEFVLLEASLLLSTMDKESISLKIKNFYEQRKT
                     TQPVNFLTAGCIFKNAQEKPAGYLIDKAGAKGLMV"
BASE COUNT          770 a          386 c          517 g          672 t
ORIGIN      
        1 tggccgaaaa tagtaaaaag ttgggcagac gggaagcagc tgaggatatt gtaaatacta
       61 tttatgatag tttatgagta taaacttctc cttgaaaaac tatttaaata acaaaaaaaa
      121 tagtgtaaac tattaggtaa agaatagttc tcctataaaa aactggaaag gttgttatga
      181 ataattcagg aaagcgtatt cattttatcg gaattggtgg ggcggggatg agcggaattg
      241 cttctctgat gctggagtta ggatatcaga taagtggttc tgatttaaac agtaatatat
      301 caactgagag gctgatagac aaaggcgctc tgatttataa aggccatgat ggcagacatg
      361 tgttgggggc caatctggta gtcatatcct cggcaatcaa taaagaaaac caggaagtta
      421 ttgctgcccg taaaaacaat attgaaatta tcccaagagc agaaatgctc tcccgtctta
      481 tgaatcaaaa atatcgtatt gctgtttccg gtacccatgg caagaccacc actacctcta
      541 tgatcagtct attactggag ggaatgaatt tagatcccac cattgctatc gggggtgaag
      601 tcaatgatat tgggggaaat gccaagctgg gcagaggaga ttatgttgtg gtagaagcag
      661 acgagagtga tggttctttt ttattactca atcccaatat tgccattata accaacattg
      721 aaaatgatca tttagattat tttaccaacc tggaaaagac tatggaaagt tttcaacagt
      781 tcatagaaaa gatacctgaa acagggtctc ttctttacgg atatgattgc cccaatacca
      841 gaatggtagc tgaaagagtg gcaggtaaaa aaaatgttat ttcttatggt tttcaccaga
      901 atgctaaagt gcgggcggca aatacctgcc tgtcaaaaaa aacttctctc tctgaaatct
      961 attttggaca acacaaattg ggtaatttga aactgaatat tcccggttat tataatgttt
     1021 ccaatgcctt agctgccatt gccacctgtc tgcaactggg tctgaaattt caggatatgg
     1081 cagagatatt gtatcatttt aacggtgtga aaagaaggca ggaaattatt tttcgactta
     1141 atgatgaatt tatgattatg gatgattatg gtcaccatcc ttctgaaata aaagctacct
     1201 tgcaggcaat aaggctgggg tggcctgagt gcaggattat taccatattt cagccacatc
     1261 gctacagcag aaccaaactg ctcttagatt attttggtac tgcctttcag gattctcata
     1321 ttgttatagt caatgaaatt tatgccgcca atgaaaaacc tatcccaaat atttctgccg
     1381 agctaattta tcaaaaaata aaaaaagagc agggagaaaa tatttattat cttcccggca
     1441 gagaagatac agtctctttc ttaagaggta ttatagcccc cggggatatc gttattacta
     1501 tgggagctgg agacgtatgg aaagtgggca cagaattagc aaaaaaaata aagggtttta
     1561 ataagaatag aggaatagac tgctgatgta taataaaaac cagatttgga gagagataat
     1621 aagcaatggc ctggaaaagg gaatcagctg taatgaacca atgaaaaagt atacttcatg
     1681 gaaaatcggt ggtaatgccg attttttttg tactccctcc catcaggaac agctggccaa
     1741 gttgttgctg tttgctctgg agcatagttt accgatatat ataataggca atgggaccaa
     1801 tatctgggtg tctgatgaag atatcagggg tctggtagtt agaattgccc atactctcga
     1861 caaagttgaa tattctgata aaatgataaa aacaggtgct ggcatcctct tgccagctct
     1921 ggcaaaaaag gcggcagata gaggactgag cgggcttgaa ttcgctgctc atattcctgg
     1981 tactttaggt ggcgccattt taaataatgc cagttttgga aaagaatcca tatctgatat
     2041 tgtcagtgaa attaccctgc tgcactatcg gaaaggaaac attgtgaccc ttaagagggg
     2101 acaattctcc tttttttata gagaaatcga tctgggctac aatgaatttg tgttattaga
     2161 ggccagtctg cttctttcta ctatggataa ggaaagtatt tctttaaaaa taaaaaattt
     2221 ttatgaacaa aggaagacaa cacagccggt caacttttta acagcgggct gtatttttaa
     2281 aaatgctcaa gagaaaccgg caggttatct tattgacaaa gcgggagcga aaggattgat
     2341 ggtgg
//
LOCUS       DAOQWT010000251         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_51695_length_2332_cov_2.689943, whole genome shotgun sequence.
ACCESSION   DAOQWT010000251 DAOQWT010000000
VERSION     DAOQWT010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51695_length_2332_cov_2.689943"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1260
                     /gene="gcvT"
                     /locus_tag="PKJ95_05460"
     CDS             136..1260
                     /gene="gcvT"
                     /locus_tag="PKJ95_05460"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNR65720.1"
                     /translation="MNDSNLLRTPLFEEHLQLKGRMIPFDGFDMPVQYSGIIDEHLAV
                     REKIGIFDISHMGQIEIKGIDALPFVDYLLTNQFSDMKDDSIKYSPMCYFHGGQVDDL
                     FVYKISNRFILLIVNASPNYSEKDEQWIREQAGNYDIEIHNKSNEYGAVAIQGPLAEK
                     LLNPFFKGDISLTDLKRFKFVYGKIFGKEVLISRTGYTGEDGFEIYTAKGDDIVDIWR
                     NSLQEGDKYGIKPCGLGARDTTRFEVCYWLYGNDIDETVNPLESGQGWTVKLNKKNFI
                     GKDALLKIQQDGIKRKLTGLYIAQGGIPRHGMEVLFDNKKIGYITSGNYCPTLKKVYA
                     MAYLDLPFNKKSNMVQVLLRNKKIEAEVVDMPFLAPFNKR"
     gene            1500..>2332
                     /locus_tag="PKJ95_05465"
     CDS             1500..>2332
                     /locus_tag="PKJ95_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HNR65721.1"
                     /translation="MTAIMELLDKINAFAWGPPMMVLLVGTGILLTIRVKGVQFTKMG
                     HAWKVTFQGCFKKDLEMRGEGEITPFQSLCSALSATVGNGNIAGVATAIAAGGPGAAV
                     WMWITALFGMATKLGEATLGVKYRIKDKDGVYVGGAMYYITNGLHQKWLGWIFALFGA
                     LAAFGIGCMVQTNSMALVGLSVFKIPMWITGAVLTILTWLVVIGGIKRIGEVTELLVP
                     FMAIFYVVGALIVIISKIDMLPWAIGEIFRSAFTGRAAFGGFAGATVAQAMRYGVARG
                     I"
BASE COUNT          728 a          341 c          519 g          744 t
ORIGIN      
        1 ataagcagaa ctgatgatgc tatggcagtc aggagaccta ttttaaccta tgatgatgaa
       61 ttaatactga aggatgagtt aaattatcaa gaaaaagata tcaattttta ataaaattta
      121 atctggaggc ttaagatgaa tgatagtaat ttattaagaa cccccctgtt tgaggaacat
      181 cttcaactca aaggaagaat gattcctttt gatggattcg atatgccagt tcaatatagt
      241 ggtattattg atgagcattt ggcagtcaga gaaaagatag gcatttttga tatatcccat
      301 atgggtcaga tagagattaa aggcatagat gccctgccct ttgtagacta tctattgact
      361 aaccaattct ctgatatgaa agatgattct atcaaatact cacctatgtg ttattttcat
      421 ggcggccagg ttgatgatct ttttgtatac aagattagta atcgctttat acttttaata
      481 gtcaacgcct ctcccaatta ttccgaaaaa gacgaacaat ggattagaga acaggctgga
      541 aattatgata ttgaaataca caacaagagt aatgaatacg gggcagtagc tattcagggt
      601 cctttagccg agaaactgct aaatcctttc ttcaaaggtg atatttcttt aaccgattta
      661 aagagattta aatttgttta tggtaaaata tttgggaaag aggttttaat atcacgcacc
      721 ggctacaccg gagaagatgg ttttgaaatc tacacggcta aaggtgatga tattgttgac
      781 atctggcgca actctctcca ggaaggcgat aaatatggta taaaaccttg tggtttagga
      841 gcaagggata caactagatt tgaagtttgt tattggctat atggcaatga tattgatgag
      901 actgtaaatc ctttggagag tggccagggt tggacggtta agctgaacaa aaaaaatttt
      961 atcgggaaag atgctttact gaaaattcaa caggatggaa tcaaacgaaa gttgaccggt
     1021 ctttatattg cacaaggcgg tataccacgc catggtatgg aggtactttt tgataataaa
     1081 aaaattggct atattacctc tggtaattat tgcccaacct taaagaaagt ttatgcaatg
     1141 gcttatctgg atctcccttt taataaaaaa agtaatatgg tacaagttct attaagaaat
     1201 aaaaaaatag aagctgaagt tgttgatatg cccttccttg ccccatttaa taagagatag
     1261 aatttttgat gaataaaaag taattaagga ggtgtttttt agaatagagt atattcaatt
     1321 ttggatatca ggaggtggaa aaataatata tataatagtt tagtttaaca gaaaaagaat
     1381 caattttctc tatttcttgg tctataatgg ttgtaaaatt aacttatttt ttaaatattt
     1441 gtaagggcaa ataaaaataa atttataatt ttatagaaga ttaaggaggt ttctttgaaa
     1501 tgaccgccat aatggaattg ctggataaaa ttaatgcctt tgcctggggt ccacctatga
     1561 tggttttatt ggttggtacg ggaatattgt taactatacg ggtaaaggga gtacaattta
     1621 ccaagatggg ccatgcctgg aaagtaactt ttcaggggtg ttttaaaaaa gatttggaga
     1681 tgcgtggaga aggagaaatt acccccttcc agtctctatg ctctgccctt tctgccactg
     1741 tagggaatgg taatattgct ggtgtagcta ctgctatagc tgccgggggt cccggggcag
     1801 ctgtatggat gtggattacc gctttatttg gaatggctac taagttaggt gaagccaccc
     1861 tgggagtaaa ataccgtatt aaagataaag acggtgttta tgtgggaggg gctatgtact
     1921 atattaccaa tggtttacat caaaaatggc tgggctggat attcgcttta tttggggcat
     1981 tggctgcttt cggaataggc tgcatggttc agactaactc tatggcattg gtaggtttaa
     2041 gtgtttttaa aataccgatg tggattaccg gagctgttct gaccatttta acctggctgg
     2101 tagtcatcgg tggaattaag cgaatcggtg aagttactga attactggtc ccctttatgg
     2161 ccatctttta tgtagttggt gctttaatag tcatcatttc aaaaatagat atgcttccct
     2221 gggcaattgg tgaaatattc cgttctgcct ttaccggaag agctgctttt ggtggctttg
     2281 ccggagcaac agttgctcaa gccatgcgtt atggagtagc aagaggtatt tt
//
LOCUS       DAOQWT010000252         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_52145_length_2319_cov_3.611749, whole genome shotgun sequence.
ACCESSION   DAOQWT010000252 DAOQWT010000000
VERSION     DAOQWT010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52145_length_2319_cov_3.611749"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PKJ95_05470"
     CDS             complement(<1..529)
                     /locus_tag="PKJ95_05470"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNR65722.1"
                     /translation="MTNSNLWVARYTGIVMAATLVCRILGLGREMVISNQFGAGFETD
                     AFFVAFMIPNLLRSIIGEGALNTTFVPIFSGCLAHQDKKRADIFASNVLNLLIIALAL
                     IVSLGIWGAPFLVRVLASGFQQSPEKYLLTVRLTRIMFPYIIFASLAALFMGILNSYQ
                     EFFIPALAPALLNVGI"
     gene            639..941
                     /gene="rpsT"
                     /locus_tag="PKJ95_05475"
     CDS             639..941
                     /gene="rpsT"
                     /locus_tag="PKJ95_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNR65723.1"
                     /translation="MPIIKSAIKRVKISEKQLKRNSAYKSKVKTFIKKFENSLNTENN
                     DVIEQHYRQCVSVLDKAAQKGILAKNTVSRKKSLLAKKYKLYALNNKNDATQSVEK"
     gene            complement(952..1968)
                     /gene="holA"
                     /locus_tag="PKJ95_05480"
     CDS             complement(952..1968)
                     /gene="holA"
                     /locus_tag="PKJ95_05480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNR65724.1"
                     /translation="MAEKQNILNFVALQREIENNPIKPIYLIYGEESYLHKITLDKFK
                     IRFAEDKKSVNYENFFGEDLDFNRLANSLHTLPLGVSSQCVIIRELDRIKAPSLQRLN
                     SIIDELSFSDDNLIVLLFSNSKQIPQNINTKKILKYGEIVSLSKPGVTQTRQWIEEKC
                     RKADKEIDEEAIYYLQRITENDFALICNELEKLFTFLGDSIPKINKEILIKNIYGLSE
                     GNIFDFVDAVGERKTGKALSLLKKIMESGEYHPLQILAMLNRQIKLILKIKIPAGNSK
                     QNKGGKNLPYFVVKRLMSQSQKYKMDEFERIFNYLLSAEINLKTSSLLPSIVLEQLVV
                     KITK"
     gene            complement(1979..>2319)
                     /locus_tag="PKJ95_05485"
     CDS             complement(1979..>2319)
                     /locus_tag="PKJ95_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65725.1"
                     /translation="VLKLLYGKTSFLFTADIEELAEINMLYWDNLLKSDVLKVAHHGS
                     ITSSTDRFLEKVQPEVAVISVGINNFNHPHPDVIGRLEVYCQRLFRTDFNGTVLISSN
                     GQKYYIRTLR"
BASE COUNT          787 a          438 c          340 g          754 t
ORIGIN      
        1 taattcctac gtttaacaga gccggtgcca gagccggtat aaaaaattcc tgataggaat
       61 ttaaaattcc cataaataag gcagcaaggg aggcaaatat tatatagggg aacataattc
      121 tggtcagccg gacagtcaac aaatattttt ccggagattg ttgaaaccca ctggctaaca
      181 ctcttaccag aaagggtgcc ccccaaatac cgagagatac tatgagcgcc agggctatta
      241 ttagaaggtt tagtacattg ctggcaaaaa tgtcagccct ctttttatcc tgatgagcta
      301 aacatcctga aaaaatagga acaaaggtgg tatttaaggc gccttcacct ataatacttc
      361 ttagcagatt tggaatcata aatgctacaa aaaaggcatc tgtttcaaaa ccagccccaa
      421 actggttaga aatgaccatc tcccttccta aacccagaat ccggcacact aaagttgcag
      481 ccatcactat accggtgtat ctggcaaccc acaaattgct gttagtcata ttcatctccc
      541 aaaatatatc gtaaaaacat ttcacttatt atttcttgat tttaatatga aaagatgtta
      601 aaatttacca gcaaaacttt cagaaaggaa aaactattat gccaataata aagtcggcaa
      661 ttaaaagagt aaaaatttca gaaaagcaac ttaaaagaaa ttctgcttat aaatcaaaag
      721 taaaaacatt cattaaaaaa tttgaaaatt ccttaaatac tgaaaataat gatgttattg
      781 aacaacatta tcgtcaatgt gtttccgttc tggacaaagc tgcccaaaaa ggcattctgg
      841 ctaaaaatac agtttccaga aaaaaatctt tactggcaaa aaaatacaaa ctttatgctt
      901 tgaacaacaa aaatgatgcc actcagagtg ttgaaaaata ataagattgc ctcacttagt
      961 tattttcact accaattgtt ccagtacaat actgggaagt aaagaactgg tcttcaggtt
     1021 aatttcagca cttaacaaat aattaaatat tctttcgaat tcatccattt tatacttttg
     1081 agattgactc ataagtcttt ttactacaaa ataggggaga tttttacctc ccttattttg
     1141 cttagaatta ccagcaggta tttttatctt taaaattaac tttatctgtc gattaagcat
     1201 agccaaaatt tgcaagggat ggtattcacc gctttccatt atttttttaa gcaaggatag
     1261 agcttttcca gtttttcgct ccccaactgc atctacaaaa tcaaaaatat tcccttctga
     1321 cagtccatat atatttttta taagaatttc tttatttatt ttgggaatag agtcgcccag
     1381 aaaagtaaac aatttttcta gttcattaca aatcagagca aaatcatttt ctgttattct
     1441 ctgtaagtaa tagattgctt cttcatctat ttctttatct gctttcctgc acttctcttc
     1501 aatccattgc ctggtctgag taacacctgg tttggataaa gaaactattt ctccatattt
     1561 cagtattttt ttagtattaa tattttgagg tatttgtttg ctattagaaa agaggagaac
     1621 aattaaattg tcatcactaa aagataattc atctattatt gaattcaatc tctgaaggga
     1681 aggagctttt attctgtcta attccctgat aataacacat tgactgctca cacccagagg
     1741 caaagtgtgc aacgaatttg ccaatcggtt aaaatccagg tcttcaccaa agaaattttc
     1801 atagttaacc gattttttat cctcagcaaa acgtatttta aatttatcaa gggtaatttt
     1861 gtgcagataa ctctcttctc cataaatcag gtagataggc ttgattggat tattctctat
     1921 ttctctctgt aatgcaacaa aattaagaat gttctgcttt tcagccatat ttctatcttc
     1981 accttaaagt tcttatataa tacttctgac cattgctact gattaaaaca gttccattaa
     2041 aatctgttct gaataacctc tggcagtaaa cttccaatct ccctataaca tccggatgag
     2101 ggtgattaaa attgtttata cccactgaaa ttacggcaac ttctggctga actttttcta
     2161 aaaaccggtc agtgctggat gttatgctac catgatgagc aaccttcaat acatcacttt
     2221 ttagaagatt atcccaataa agcatattta tttctgccaa ctcttcaata tcagcggtaa
     2281 ataagaaact ggtcttacca tagagtaatt ttaacacaa
//
LOCUS       DAOQWT010000253         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_52183_length_2318_cov_2.742377, whole genome shotgun sequence.
ACCESSION   DAOQWT010000253 DAOQWT010000000
VERSION     DAOQWT010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52183_length_2318_cov_2.742377"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="PKJ95_05490"
     CDS             <1..120
                     /locus_tag="PKJ95_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNR65726.1"
                     /translation="GFFCAIGNCSSCLMKVNGIPNVRTCITRLEEGMIIERQC"
     gene            132..1217
                     /locus_tag="PKJ95_05495"
     CDS             132..1217
                     /locus_tag="PKJ95_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR65727.1"
                     /translation="MKEYDIIVVGGGPAGLAAATTAAKCNANILLVDDGLQLGGQLIK
                     QTHKFFGSTEQFAGIRGIDIAHNFIDEISHLSNIEILTQSTATGFFEDGVLTILEGYN
                     GNRLSKVKPRRLIIATGASENMLSFINNDLPGVYGAGAVQTLMNVYGVIPGRAVLMVG
                     ADNIGLIVSYQLLQAGIEVKAIVEALPCIGGYWVHASKIRRLGIPIYTRCTLKEVKGK
                     EEVESVTICNIDDKFNFIPGSERELSVDTICLAVGLSPLAELLWQAGCEMAYIPELCG
                     YVAKRDENMKTSLDHIYIAGDVAGIEEASSAMVEGQIAGLNAAASLGLYHEKYRELNL
                     AFHRQLLQLRHNEVSESITRGIKKATL"
     gene            1238..1738
                     /locus_tag="PKJ95_05500"
     CDS             1238..1738
                     /locus_tag="PKJ95_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNR65728.1"
                     /translation="MSVALNGVPTKEELKRVTPPEEVLAKKPVVMVECFQNIPCDPCA
                     ANCPFGAILPFADINDLPLVDYEKCTGCGICIASCPGLAIFVLDMNYSAREALIKVPY
                     EMMPLPKKGQMVSGLDRQGCKVAEVMVQKVTRTKNKTSIISIVVPKELAQIIRNIRVE
                     EAVNGR"
     gene            1728..2000
                     /locus_tag="PKJ95_05505"
     CDS             1728..2000
                     /locus_tag="PKJ95_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNR65729.1"
                     /translation="MDDNTIICRCEDVTWGEIREQLDKGYTTMDEIKRITRAGMGPCQ
                     GKTCQRILLHAIARYLNKKVEEIPLSTFRPPTKPIPLGILAGGQDD"
     gene            1993..>2318
                     /locus_tag="PKJ95_05510"
     CDS             1993..>2318
                     /locus_tag="PKJ95_05510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR65730.1"
                     /translation="MIDRAYIVIIGGGIQGCSIGYNLAKKGQKNIVILEKETVASGAS
                     GRCGAGFRQQFGTEMNCILARESVKIFEQLSDELEYDIELNQRGYLILAYTEKEVEQF
                     QKNVQL"
BASE COUNT          703 a          412 c          585 g          618 t
ORIGIN      
        1 ggtttctttt gtgctattgg taattgctct tcctgcctga tgaaggttaa tggcataccc
       61 aatgtcagga cctgcattac cagactggaa gaaggaatga tcattgaaag acaatgttag
      121 aaagggcaaa aatgaaagag tatgatatta ttgtggtagg cggcggacct gcaggattgg
      181 ctgctgctac cactgccgct aaatgtaatg caaacatttt actggtagat gatggtttac
      241 aactgggggg acagctcatc aagcagaccc acaaattttt cggctctact gagcagtttg
      301 ccgggataag agggatagat attgcccata acttcataga tgaaatatcc cacttgtcca
      361 atatcgagat tttaactcaa tcaacggcta ccggattctt tgaagatggt gtccttacca
      421 tattagaagg ttataatggt aacaggttaa gcaaggtaaa acctcgacgt ctaattatag
      481 ctaccggtgc ctctgaaaat atgctatctt ttataaataa cgatctgccc ggcgtctatg
      541 gtgctggagc ggtacagacc ctgatgaatg tctatggagt cattccaggt agagcagtgc
      601 ttatggtggg cgccgataat attggtctta ttgtcagcta tcagctcttg caggcaggca
      661 ttgaggtaaa ggctatagta gaggctctgc cctgtattgg aggatactgg gttcatgcca
      721 gtaaaataag aagattggga attcccatct atacccgttg taccttaaaa gaggtaaagg
      781 gcaaagaaga agtggaaagt gttacaatat gtaatattga tgataaattt aattttattc
      841 cgggaagcga aagggagctc tcggtggaca ccatttgcct ggcagttggt ttatctcccc
      901 tggcagaact actctggcag gcgggatgtg agatggccta cattcccgaa ttatgcgggt
      961 atgtggctaa aagggatgaa aatatgaaaa catctttgga ccatatctat attgccggtg
     1021 atgtggctgg tattgaagag gccagcagcg ctatggtgga agggcagatt gccggtctaa
     1081 atgccgcggc aagcttgggt ctgtatcatg aaaaataccg ggagttaaat cttgcttttc
     1141 accggcagct cttacaattg cgtcataatg aggtgagtga gagcatcacc agagggataa
     1201 aaaaagcaac cctttaagga gaaaaaagga ggaaaatatg tctgtagctc ttaatggagt
     1261 gccaacaaaa gaagaattaa aaagagttac tccccctgag gaggttctgg caaagaaacc
     1321 tgtggtaatg gtagaatgtt tccaaaatat tccctgtgac ccctgtgctg ccaactgtcc
     1381 tttcggggcg attttaccct ttgctgacat caatgattta ccgctggtcg actatgaaaa
     1441 atgcactggt tgtggtatat gtattgcctc ctgtcccgga ctggccatct ttgttctgga
     1501 tatgaactat tctgcccggg aagctttaat taaggttcca tatgagatga tgccacttcc
     1561 aaaaaaaggg cagatggttt ccggattaga ccggcaaggt tgtaaagtag cagaggtgat
     1621 ggtacaaaaa gtcaccagga ctaaaaacaa aacctcgatt ataagtatcg ttgtaccaaa
     1681 agaattagct cagattatac gtaatatcag agtggaggag gcagtaaatg gacgataata
     1741 ctattatctg tcgctgtgag gatgttacct ggggggaaat aagagaacag ttggataagg
     1801 gttatacaac tatggatgag attaagcgca tcaccagggc aggaatgggt ccctgtcagg
     1861 gaaaaacctg tcagagaatt ctattacatg ctattgcccg ttacttaaat aaaaaggttg
     1921 aggaaatccc gctctccact tttcgacccc ctaccaagcc tattcctcta ggaatattgg
     1981 caggaggtca ggatgattga ccgggcttat atcgttatta taggtggtgg aattcaagga
     2041 tgcagtattg gttataatct ggcaaaaaaa ggacagaaaa atattgtaat tttagaaaaa
     2101 gaaacagttg ccagtggagc atcaggaaga tgtggagcag ggtttcggca gcagttcgga
     2161 actgagatga attgtatttt agccagggag agtgtaaaaa ttttcgaaca actaagcgat
     2221 gaactggaat atgatattga actgaatcag aggggctacc ttattctggc ttatactgaa
     2281 aaagaggttg aacaattcca aaaaaatgtc cagttgga
//
LOCUS       DAOQWT010000254         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_52638_length_2304_cov_3.315251, whole genome shotgun sequence.
ACCESSION   DAOQWT010000254 DAOQWT010000000
VERSION     DAOQWT010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52638_length_2304_cov_3.315251"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..258)
                     /locus_tag="PKJ95_05515"
     CDS             complement(109..258)
                     /locus_tag="PKJ95_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65731.1"
                     /translation="MTQLNSNSIKAILQEELEKDGDFLKEMVRSIMQEVMEECLCYSK
                     TAPLY"
     gene            571..1668
                     /gene="hisC"
                     /locus_tag="PKJ95_05520"
     CDS             571..1668
                     /gene="hisC"
                     /locus_tag="PKJ95_05520"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNR65732.1"
                     /translation="MIDAKNLTRRGILDLKPYIPGKPIEEVQRELGLTEVVKLASNET
                     SVGPSPLALEAIRKELGNSNLYPEGSSRLLREKLAEKLSIGEDFIIVGNGADNVIDLT
                     GMAFINDGDEIVTSEITFPAYETITRIMGGKYITCKLKDFTFDLEAIAEKVSSKTKMI
                     YLCNPNNPTGTIVKRDAVEKLMDKIPENVIVVFDEAYYDYVEDEEYPNSLSYVLEGRN
                     VIILRTFSKIAGIAGLRVGYGIARPELIGYLSRVISPFPTNRLAQVGALASLDDSEHY
                     EKVLKSNREGKQYLYRELNKLNIFYVPTETNFIFIDLKEDSQSFYEKCLQRGVIIRPG
                     KTWGYPNCIRLTIGTQYENEKFVHALKEIIT"
     gene            1684..>2304
                     /locus_tag="PKJ95_05525"
     CDS             1684..>2304
                     /locus_tag="PKJ95_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNR65733.1"
                     /translation="MIKLLTGNEAVALGAIEAGVDVVAGYPGTPSTEVMLNLMAKRKE
                     LGIHMEWSVNEKVAFEIAAGAAWVGKRALVTMKMSGLNVAADSISAIAYSGCTGGLVI
                     FVADDPGTSAGMVEQDSRYYAKSMIIPMLDLASQQECLDYMKIAFDISEAIKGPVMLR
                     STTDISHVASLVDINGQTGRENKKREAHFERNIPKYTKAASAWCMAQ"
BASE COUNT          753 a          369 c          479 g          703 t
ORIGIN      
        1 ttaatgttag agaaagtgtc ttaactttgt gtccggatta tcgggggaag accagttctc
       61 ttatgttcat acatagggta tgctacttta aagtggtgca cttttcaatt aatacagtgg
      121 tgcagtttta gagtaacata agcattcttc cataacctct tgcataatac ttcttaccat
      181 ctcctttagg aaatctccat ccttttctaa ttcctcttgc aaaatagcct ttattgaatt
      241 actatttagt tgggtcatct tgaatttatc ctccttaatg attttagttt cttttaatta
      301 ttatggaggt tcttgatggc ccatttcaag ttaatcttta attaactaat cttactttta
      361 cagaaaatat aacacacact cgttaactat tatataattt ttataaaatg ctttacataa
      421 ataaaaaatc gtgctaaaat aataatccga aagtttttaa tatttgccca aagttataag
      481 tcattagggg gagccgtaac tccccctttt ctgttttaat caggcaagtt caagttatag
      541 ataaaataaa taaaaaagag gagggaagaa atgattgatg caaagaatct taccagaagg
      601 ggtattctgg atttaaagcc ttacattcct ggtaaaccaa tagaagaggt gcaaagagaa
      661 ttaggattga cagaagttgt gaaattagct tccaatgaaa cttctgtagg cccgtctcct
      721 ttagccctgg aggcaatcag gaaggaattg ggtaatagca atctttatcc cgaaggctca
      781 agcagattgt taagagaaaa gttagctgaa aagttgagta tcggtgagga ttttataatc
      841 gttggtaatg gagcagacaa tgttatcgat cttaccggaa tggcttttat taatgacggt
      901 gatgaaattg ttacctcaga aattactttt cctgcttatg aaactattac cagaataatg
      961 ggtggaaagt atattacctg caaattaaaa gattttacct tcgacttaga agctatagct
     1021 gaaaaagttt ccagcaaaac caagatgatc tacctttgta atcctaataa tcctacaggt
     1081 acaattgtaa aacgagatgc agtagaaaag ttgatggata aaattcctga aaatgttatc
     1141 gtagtgttcg atgaagctta ttatgattat gtagaagatg aagaatatcc gaatagcctt
     1201 tcttatgtct tagaaggaag gaatgtgatc attttgcgta ctttttcgaa gatagcaggt
     1261 atagcaggat tacgggtagg gtatggtatt gcaaggcctg aattaatagg ttatttaagc
     1321 agggtaatca gtccctttcc tactaatcga ctggctcaag ttggcgccct tgcctctctc
     1381 gatgatagtg agcattatga aaaggtacta aaatcaaacc gagaaggaaa acagtatctt
     1441 tacagagagc tgaacaaact aaacatattc tatgtaccaa ctgagaccaa ttttattttt
     1501 attgacttga aagaagatag ccaatcattt tatgaaaagt gcttacaaag aggggttatt
     1561 attcgacctg gcaagacctg gggttatccc aattgtattc gtttaaccat tgggacccaa
     1621 tatgagaatg aaaaatttgt ccacgcctta aaagaaataa tcacataaaa gaggtgaaga
     1681 aatttgataa aattattgac cgggaatgaa gctgttgctc taggagcaat agaggcagga
     1741 gtagacgtag ttgcgggata tcctggtact ccttccacag aagtcatgtt aaacctgatg
     1801 gcaaaaagaa aagaattagg tattcatatg gaatggagcg taaatgaaaa agtagccttt
     1861 gaaatagctg ctggtgctgc ctgggtagga aaaagggcac tggtcacaat gaagatgtca
     1921 ggtttgaatg tggctgcaga ctctatctca gctattgcct atagtggttg tactggagga
     1981 ttagttatct ttgtagccga tgatccggga acaagtgccg gaatggtaga gcaggattcc
     2041 agatattatg ccaaatcaat gattattcca atgttagatc ttgcttccca acaggaatgc
     2101 ttagactata tgaagattgc atttgatatt tctgaagcca ttaaaggtcc tgttatgtta
     2161 agatctacaa ccgatatttc ccatgttgcc tctctggtgg atataaatgg acagacggga
     2221 agggaaaaca aaaagagaga ggcccatttt gagcggaata taccgaaata taccaaagct
     2281 gcctctgcct ggtgtatggc tcag
//
LOCUS       DAOQWT010000255         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_52756_length_2301_cov_3.329475, whole genome shotgun sequence.
ACCESSION   DAOQWT010000255 DAOQWT010000000
VERSION     DAOQWT010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52756_length_2301_cov_3.329475"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="PKJ95_05530"
     CDS             <1..1411
                     /locus_tag="PKJ95_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNR65734.1"
                     /translation="RLEEKKIIGNIYRGKIMNVLPGIEASFIDIGIDRNAFLHLNDVI
                     QSFGRKSDKDIDIHKLIKVGQEIDVQVVKEAIMPKGAKVTTNISLPGRYLVLMPNNNT
                     IAVSRRIEQEEERERLKKIVHKIKKNEYGFIIRTAATGKEEEDLLPDMDFLVRLWNKI
                     QKRGKRAKAPLLLHEDLSLTYRVIRDLFTEEIDEFLVNSKQEYEDIINYLDNLFLLEL
                     KPRVSYYEGDKPIFDAFNIEKEISKALEKKIWLKCGGYLVFDEVEALTVIDVNTGKYV
                     GGKKMRDTILKTNLQAAEEIARQLRLRYIGGIIIIDFIDMDNKEDQEKVINKLENELK
                     KDRIKSNIVQTTELGLVEMTRKRSKRDLDSLLRTSCPYCSGSGRIMSPETVSNQVLHK
                     LEDLGKNSKAEAILLGVNPDVEEALSGGKMLLIKQLERDYKKVVYIRPLPYLHIEKIK
                     VIAVGKKRDVKKILKVLQD"
     gene            1575..1886
                     /gene="rplU"
                     /locus_tag="PKJ95_05535"
     CDS             1575..1886
                     /gene="rplU"
                     /locus_tag="PKJ95_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNR65735.1"
                     /translation="MHAIITTGGKQYLIKEGDIIKVEKLKLDEGEKVKFEQVLMLEKD
                     GKYSFGQPLIENSFAEGKILRHGKAKKILVLKYKPKKRYEKKMGHRQLFTEIRIEKIN
                     I"
     gene            1942..2208
                     /gene="rpmA"
                     /locus_tag="PKJ95_05540"
     CDS             1942..2208
                     /gene="rpmA"
                     /locus_tag="PKJ95_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNR65736.1"
                     /translation="MAHKKGQGSAKNGRDSISKRLGVKIYGGQFVRSGNIIVRQRGTR
                     ILPGKNVGRGGDDTLFALIDGYVTFKTTGKKRKQVSIASSESEA"
BASE COUNT          902 a          272 c          479 g          648 t
ORIGIN      
        1 aagattggaa gagaaaaaaa ttattggcaa catttataga ggtaagatta tgaacgtttt
       61 gcctggtata gaggcttctt tcatcgatat aggaatagat agaaatgcat ttttacatct
      121 caatgatgtt attcaatctt ttggtcgaaa gagtgataaa gatattgata tacacaaatt
      181 aattaaagta gggcaggaaa tagatgttca agtggtaaaa gaagcgatta tgccgaaagg
      241 tgctaaagtt actactaata ttagccttcc aggacgttat cttgttctga tgcctaacaa
      301 taataccatc gcagtttcca gaagaattga acaagaagaa gaaagagaaa gattaaaaaa
      361 aattgttcac aaaataaaga aaaatgagta tggttttatt attagaactg ctgccacggg
      421 caaagaggaa gaagacttat tacctgatat ggattttctt gtccgtttgt ggaataaaat
      481 tcaaaaaaga ggaaaaagag ccaaagcacc cttattactg catgaggatt taagtctgac
      541 ttaccgggta attagagatt tatttaccga agagattgat gaatttctgg tgaattctaa
      601 acaagaatat gaagatatta taaattatct tgataaccta tttttattag aacttaagcc
      661 cagagtttct tactatgaag gagataaacc tatattcgat gcttttaata ttgaaaagga
      721 aataagtaaa gcattggaaa aaaagatttg gttaaaatgt ggaggttatc ttgtttttga
      781 tgaagtagaa gctttaacgg taattgatgt aaatactggg aaatatgttg gtggcaaaaa
      841 aatgcgtgat actattttaa aaaccaatct acaggcagct gaagaaatcg ccagacaatt
      901 gcgattaaga tatattggtg gaattattat aattgatttt atcgatatgg ataacaaaga
      961 agaccaggaa aaagtaataa ataaattaga aaacgaattg aaaaaagata gaataaaatc
     1021 aaacattgta caaactacag aattaggatt agttgagatg accaggaaac gctccaaacg
     1081 cgatttagat tcactattac gaacctcctg cccttattgc tcaggaagtg gcagaataat
     1141 gtcaccagaa acggttagca atcaagtatt gcacaaatta gaagatttgg gaaaaaattc
     1201 caaagcagag gctattctat taggggtaaa tcctgatgtt gaagaggcgc tatcgggtgg
     1261 aaagatgtta ttaataaaac aattagaacg tgattataaa aaagttgttt atattagacc
     1321 attaccatat cttcatattg aaaagattaa agtaattgct gtagggaaga aaagagatgt
     1381 aaaaaaaata ttaaaagtat tgcaagattg aagttgttat tcaaaatgtt caagttatca
     1441 ctatttgata agaaaataat ttgacaggat tatccatata tgataaattt tataggctat
     1501 atacgcactg ataaggttaa aaagctaagg ctaccgattc aggcgattta tttaagcagg
     1561 aggagttaac gaagatgcat gccataatta caacgggggg gaagcagtat ctaattaaag
     1621 aaggcgatat aataaaagtt gaaaaactga aattggatga aggggaaaag gtaaaatttg
     1681 aacaggtatt gatgttggaa aaagatggta aatatagttt tggtcagccc ttaattgaga
     1741 atagttttgc tgaaggtaaa attttaagac atggaaaagc aaaaaaaata ttagtattaa
     1801 aatataaacc aaaaaaaaga tatgaaaaaa agatgggaca ccgacaatta tttactgaaa
     1861 taagaataga gaaaataaat atttaatgga ttttattacg aattgaaggt taccaaaagt
     1921 agttaagtga ggtgagacta aatggctcat aaaaaaggac aaggctcggc aaaaaatggt
     1981 cgcgatagta tttctaaaag gcttggtgtt aaaatatatg gtggacagtt tgttcgtagt
     2041 ggaaatataa ttgttcgaca aagaggaacc agaatacttc ccggtaaaaa cgttggccgt
     2101 ggaggggatg ataccttatt tgctcttatt gatggatatg tcactttcaa aacaacgggg
     2161 aaaaaaagaa aacaggtgtc cattgcctct tctgagagcg aagcgtaaac tgacttttaa
     2221 acccctgagt caagaataaa ctcaggggtt tttttatgta tgacgggcat gccgtcaatc
     2281 tgtggtgaaa gtccacaagg g
//
LOCUS       DAOQWT010000256         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_52803_length_2300_cov_2.567038, whole genome shotgun sequence.
ACCESSION   DAOQWT010000256 DAOQWT010000000
VERSION     DAOQWT010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52803_length_2300_cov_2.567038"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /gene="rpsB"
                     /locus_tag="PKJ95_05545"
     CDS             <1..612
                     /gene="rpsB"
                     /locus_tag="PKJ95_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNR65737.1"
                     /translation="RNNIYIIDLQQTIKLIDDAYNFVRDLVSKNGTILFVGTKKQAQE
                     SIYSEAEKCGMPYINYRWLGGTLTNFNTINQRVKRLYQLEEMDKSSQFSLLPKKEIII
                     LKREQEKLERLLTGVKNMDKLPDALFIIDPKRERNAVTEAKKLSIPIVAVVDTNCNPE
                     EIDYIIPGNDDAIRAIKLLASVIADAVIEGKKILQEDNIKIAV"
     gene            655..1248
                     /gene="tsf"
                     /locus_tag="PKJ95_05550"
     CDS             655..1248
                     /gene="tsf"
                     /locus_tag="PKJ95_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNR65738.1"
                     /translation="MAINAEQVKKLREKTGAGMMDCKKALIHTEGDLEKASDYLRSKG
                     IQAASSKLGRKALNGIITSYIHLNDRVGVLLEINCETDFVAKTQEFKELAKNITLQIA
                     AANPRYVSRADVPQDVIEKEKEIYRNQLTDSKKPEKVIDNIVEGKLNKFFKEICLVEQ
                     DYIRNPEITVQQLILETISKLKENIQIKRFVRYQLGE"
     gene            1335..2060
                     /gene="pyrH"
                     /locus_tag="PKJ95_05555"
     CDS             1335..2060
                     /gene="pyrH"
                     /locus_tag="PKJ95_05555"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNR65739.1"
                     /translation="MKNKQSIYKRILLKLGGESFSGEKRFGIDIKRVNFISSELAEIK
                     QMGVQIAVVIGGGNIFRGKEASVKGINQVSADYMGMLGTVINALALQDSLEKLNIETR
                     VQTAIEMKAIAEPYIRRRAIRHLEKGRIVILAAGTGHPFFTTDTAAALRAIELEAEAM
                     LKATKVDGIYEKDPLLDSNALKYKKINFLEFINKNLKVMDATSISLCMENKVPVIVFN
                     LNLPGNIKKVVMGEPIGTIVKED"
     gene            2063..>2300
                     /locus_tag="PKJ95_05560"
     CDS             2063..>2300
                     /locus_tag="PKJ95_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNR65740.1"
                     /translation="MLTEIIKETEHKMKKAIDITQQELALIRTGRASTALVDKIKVPY
                     YGVLTSLKEIANITVPESDLIVIQPWDKNFINEVE"
BASE COUNT          887 a          309 c          442 g          662 t
ORIGIN      
        1 agaaataata tttacattat agatctacag cagactatca aacttattga tgatgcctat
       61 aattttgtac gcgacctggt cagtaaaaat gggaccatcc tgtttgtggg tacaaaaaaa
      121 caagcacaag agtctattta ttctgaagct gaaaaatgcg gcatgcccta tattaattac
      181 agatggcttg gtggtacctt aactaatttt aatactatta atcaaagggt gaaacgtctt
      241 tatcaattgg aagaaatgga taaaagcagt caattttctt tattaccgaa aaaagaaata
      301 atcattttaa aacgagagca agaaaagttg gaaagattgc tcaccggtgt caagaatatg
      361 gataaattgc cagatgcttt atttattatc gatcctaaaa gagaaagaaa cgcagtaact
      421 gaagcaaaaa aattatctat acctattgtg gcagttgtcg atacaaactg taatccagaa
      481 gagattgact acataattcc aggtaatgat gatgctataa gagcaattaa actattagcc
      541 tctgtaatcg cagatgctgt aatagagggt aaaaaaatac ttcaggaaga taatattaaa
      601 attgccgtat agtatagaat attcaagact aacgattaag gagagctggt aaaaatggct
      661 attaatgctg aacaggttaa aaaattaaga gaaaaaacag gtgccggtat gatggattgc
      721 aaaaaagctt tgatacatac cgagggagat ttagagaaag catctgatta tcttcgttca
      781 aagggaatac aggcggcaag ctcaaagttg gggcgtaagg cgctaaatgg tataattaca
      841 tcctatattc atttaaatga cagagtaggt gttcttctgg aaataaattg tgaaactgat
      901 tttgttgcta aaacacaaga atttaaagaa ctggcaaaaa atattactct tcagatagct
      961 gccgcaaatc ccagatatgt cagcagggca gatgtacctc aggatgttat tgaaaaagaa
     1021 aaagaaattt accgcaatca attaactgat agcaaaaaac ctgaaaaagt aattgataat
     1081 atagtagaag gaaaactaaa taaatttttt aaagaaatat gtttagttga acaagactat
     1141 attcgtaatc ccgaaataac tgttcaacaa ttgattttgg aaactatatc caaattaaaa
     1201 gaaaatattc agataaagag atttgttaga tatcagcttg gcgagtagaa gtagaatata
     1261 tctataataa aaaatatttt acttaaacta ttttatattt ttaatatata aaagaataaa
     1321 caaatttaga gaaaatgaaa aataagcagt ctatttataa aaggatattg ttgaaattag
     1381 gcggagagtc tttttcaggt gaaaaaagat ttggcattga tattaagcgg gttaatttta
     1441 tttcctccga attagccgaa ataaaacaga tgggggttca gattgctgtt gtcatagggg
     1501 gaggcaatat ttttagaggc aaagaagcca gcgttaaagg aattaaccag gtttctgccg
     1561 attatatggg gatgcttggt acggtaatta atgcccttgc cttacaagat tctttagaaa
     1621 aacttaatat tgaaacaaga gtacaaaccg ctattgaaat gaaagcaatt gccgaacctt
     1681 acattagaag gagggcaatc agacatttag aaaaaggaag aatcgttatc cttgcagcgg
     1741 ggactggtca tccctttttt actactgaca cagctgcagc tttaagggct attgaattgg
     1801 aagctgaagc tatgttaaag gctactaaag tggatggcat ctatgagaaa gacccgcttt
     1861 tagattctaa tgctttaaaa tataaaaaga taaatttcct tgaatttata aataaaaatt
     1921 taaaagtaat ggatgctaca tctatttcat tatgtatgga aaataaagtt cctgtgattg
     1981 tatttaatct aaatttacct ggaaacatta aaaaggtagt tatgggagaa ccaattggaa
     2041 ccattgttaa ggaggattga atatgctgac agaaataatt aaagaaactg aacacaaaat
     2101 gaaaaaagcc attgatataa cccagcagga attagcactt attagaacag ggagagcctc
     2161 aactgccctg gtggataaaa ttaaagttcc atattatggg gttttgacct ctttaaaaga
     2221 aattgctaat attaccgttc cggaatcaga tttaattgta attcaaccct gggataagaa
     2281 ttttattaac gaagtagaga
//
LOCUS       DAOQWT010000257         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_53071_length_2292_cov_3.212785, whole genome shotgun sequence.
ACCESSION   DAOQWT010000257 DAOQWT010000000
VERSION     DAOQWT010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53071_length_2292_cov_3.212785"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /gene="ilvB"
                     /locus_tag="PKJ95_05565"
     CDS             complement(<1..1671)
                     /gene="ilvB"
                     /locus_tag="PKJ95_05565"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNR65741.1"
                     /translation="MELTGTEIIVECLKLEKVRVIFGIPGAAIMPLYDTFGKYEELPF
                     KNVLSRHEQGAIHAADGYARATGEVGVCVVTSGPGATNLVTGLANAHMDSVPLVAITG
                     QVPTAMVGNDAFQEVDITGITLPITKHNYIARKVEDLPLIMKEAFYIARTGRPGPVLI
                     DLPKDVQLSKSNFNYPEKLNKRGYNPSYKGNLKQIKSAAIKIMNARKPIIIAGGGVIN
                     SKSSMELLKIVDKTNIPVVNTFMGLSSFPKNHPLFLGMLGMYGQYAANQSVSNADLII
                     ALGTRFDDRITGNLNQFCRLTEIIHIDIDPAEIGKNIFAKIPIVGDLKYILQQLNNYL
                     EEKKEDAWLTEINNIVEKDPSRKIENGSLLSSRFILESLNNFFAGEAIIVTDVGQHQM
                     WAAHYAQVNFPRHFISSGGLGAMGFGFPAAIGAKIGCPEKQVICIAGDGGFQMNIQEL
                     ATAIHNRLPIIVLLMNNGYLGMVRQLQELFCDKRYVSTLLAGNPDFVKVVESYGGIGY
                     RVDKREEFMPILQEAHCKNEFVLIDCHIPQEENIFPLVKPDASINQMIG"
BASE COUNT          685 a          479 c          357 g          771 t
ORIGIN      
        1 tcctatcatt tgattgattg atgcatcggg ttttaccagt ggaaagatat tttcttcctg
       61 aggtatgtga caatctatca acacgaattc atttttacaa tgcgcctctt gtaatatagg
      121 cataaactct tctcttttat caacacgata accaatacca ccataactct ctaccacttt
      181 tacaaaatca gggttaccgg ctagtaaggt agaaacatat cgtttatcac agaacaattc
      241 ctgtaattgt ctgaccattc ccaaatatcc attattcatt aatagtacaa taatcggtaa
      301 acggttatgg atggcagtgg ctaactcctg gatattcatt tgaaaccctc catcccccgc
      361 aatgcaaata acctgttttt cggggcaacc tattttagca cctatagcag caggaaaacc
      421 aaatcccatt gccccaagac cacctgaaga gataaaatgg cggggaaaat tgacctgagc
      481 ataatgagct gcccacatct gatgctgccc tacatcggtt acaataatgg cttcaccagc
      541 aaaaaaattg ttaagactct ccagaatgaa tcttgagctg agtaagcttc cattttctat
      601 tttcctcgaa gggtcctttt ctacaatatt attaatctca gttaaccagg catcctcttt
      661 tttctcttcg agataattgt tcaattgctg taagatatat ttcagatcac ccactattgg
      721 tatctttgca aaaatatttt tgcctatttc agccggatca atatcaatat ggattatttc
      781 agtaaggcga caaaattggt taagattccc tgttatgcga tcatcaaagc gggttcccag
      841 agcaataatc aggtcggcat tactaacaga ttgatttgca gcatactgac catacatacc
      901 taacattcct agaaagagag ggtgattctt cggaaaacta ctaagaccca taaaagtatt
      961 gaccaccgga atatttgtct tatctactat cttcaacaat tccatgcttg atttgctatt
     1021 tataacaccc cctccggcaa taattatagg tttacgggca ttcattattt taattgcggc
     1081 tgattttatc tgtttaagat tccctttata tgaggggtta taaccccttt tattcaattt
     1141 ttccggatag ttgaaattac ttttagataa ttgaacatct tttggtaaat caataagaac
     1201 gggtcccggc ctgccggttc tagcaatata aaatgcctct ttcatgatca agggtagatc
     1261 ttcaactttc ctggcaatat aattgtgttt agttatgggt agggttatgc cggtgatatc
     1321 aacctcctgg aatgcatcat tcccaaccat tgctgttgga acctgtccgg taattgctac
     1381 tagtggtact gaatccatat gggcattagc taggccagta accagatttg ttgcccccgg
     1441 gccggaagtt accacacaaa caccgacttc accagttgcc cgggcataac cgtcagcagc
     1501 atgaattgct ccttgctcat gtctgcttaa aacattttta aaaggaagtt cttcgtattt
     1561 gccaaaggta tcgtataaag gcattattgc tgcaccgggt ataccaaata tcactctgac
     1621 tttctccagt ttcagacatt caacaattat ttctgttcca gttagttcca ttttctctct
     1681 ccatttcttt cctcatatca ccaattattt aactatttaa ttgcttaagt tgctattatt
     1741 cttaataagt ataaaattat aaagacccct gcagaggttc cttgctttta actaaggaac
     1801 cttccgctgg ggtctttttt acccacggtg taatacgcat tttttagaat ttttaaaaac
     1861 gtatctatac cactcggtcc agacttttct taaaaaacgg aaccctaggt atactccctt
     1921 attgtaaata aaaaagtcat ccccaggact ttaatcctat agggacgact ttatcgtgtt
     1981 accaccctat tttagtagaa aactatttat tttctaccct caattaaata tgcaataaat
     2041 ataataaaaa tcattgataa taactacggt tgttattaac acattttcaa aaagaagtga
     2101 gtttcagcaa cttttactac cctatcacac cagtatcggg ttctctttag taaattattg
     2161 cttacttgct tcttaattaa gtaatttttg aaattctact aatataattt tgttttgtca
     2221 aataatattt aaaattaatg tgattaattt gtgataatgt caccttatta attatcttga
     2281 taaaatgtca ct
//
LOCUS       DAOQWT010000258         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_53076_length_2292_cov_2.976755, whole genome shotgun sequence.
ACCESSION   DAOQWT010000258 DAOQWT010000000
VERSION     DAOQWT010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53076_length_2292_cov_2.976755"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            580..792
                     /locus_tag="PKJ95_05570"
     CDS             580..792
                     /locus_tag="PKJ95_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65742.1"
                     /translation="MFVIIKVINNFCNVLSILEDIWLIPYNQSVCMKKLDGAYLYDLS
                     SGKARDEYIFIQFTHSCLLIPKVKKE"
     gene            complement(871..1494)
                     /locus_tag="PKJ95_05575"
     CDS             complement(871..1494)
                     /locus_tag="PKJ95_05575"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HNR65743.1"
                     /translation="MFINHFLNKKMKVVAYLCTILLFVILALLTLSNAVGADETVPLP
                     KGGLPIIITTAGQSPGAISISVICRRNKIDNDYLNLATVEDLKAKEYKTLIVTMGTSG
                     KGMGAAGIEINDELARIEKLIEEARKQNMLILGFHIEGEARRSGACEQSIDLVGPRVD
                     YLVVRSDGNLDGRFDEIAKKNNIPLRIFDESIELNEILKEIFLKDEG"
     gene            complement(1661..>2292)
                     /locus_tag="PKJ95_05580"
     CDS             complement(1661..>2292)
                     /locus_tag="PKJ95_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNR65744.1"
                     /translation="MASSVIIGVADMYETLCERFIEDSKNIIVADPLDPKFINETLVM
                     GPVISKKAKDFILHMIEEGIKEGATLALDGRGVVVPGCEKGYFIGPTVFTDVKPKMKI
                     HDVEIFGPVVCIMKADNLDEAIQIINDHKYGNGSSIYTQNGYYARKFKLEVEAGMIGI
                     NIGIPAPVAYLPFGGMKESQFADIKAQGKAIINFFTEDKIITERYFKEG"
BASE COUNT          702 a          441 c          302 g          847 t
ORIGIN      
        1 tcatgtttct gtcttggaac gattacaata ttcggattac atgcccattc ttgtgatccc
       61 atattcatta cagaaccgtc aacagaggca tcttttccga cactcatatt agtacagcta
      121 aaagtagtta agtttgcgaa gatcagtatc agtgccatta agctaactga tattaaaatt
      181 ctatacttag atttcatgaa attctcctat tttttgataa ttttctttta ttagcttact
      241 attttttact taagttgtag aactgattac cttttataat cacctcccat ccttttaatt
      301 gttagttacc ctggatcagt tagattttta tgaacggtac attgtaacta ctatatctag
      361 ttaatctatc accatgaatt attaatagta ctatcaaata aagatgagct aaattaaata
      421 ttatccaaat taaaagtaat ttcttctata atctaaagta taatctccct tccatgttag
      481 tttctatttt cacaaaaaga tgttttacaa tcttgattcc gagactgaat atatatctca
      541 caataggttt gatttgggaa actcttcttt ggtaaatctt tgttcgttat cattaaagta
      601 attaataatt tttgcaatgt gctctctatt ctagaagata tctggcttat cccttacaat
      661 caatctgtct gtatgaaaaa gttagatggt gcttatttat atgatctttc atccggaaaa
      721 gccagagatg agtatatttt tattcagttc actcattcct gtttattaat tcctaaagtt
      781 aaaaaagaat aattttttaa aatttcagct acttaaaata gtatcccatg ctacttatag
      841 tttggactgt tttaaaactt actctatttt ttaaccttca tcttttaaaa atatttcctt
      901 aagtatttca tttaactcta tactttcatc aaatattctt aaaggaatgt tgtttttctt
      961 cgctatttca tcaaatcttc catccaggtt gccatcactt ctaacgacta aatagtctac
     1021 tctcgggccg actaaatcaa tagactgttc gcaggcacca cttcttctcg cttcaccttc
     1081 tatatgaaat cccaaaatta gcatattctg ttttctggct tcttcgataa gcttttcaat
     1141 tcttgctaat tcatcattta tctctattcc agctgcaccc attcctttac cactggtgcc
     1201 cattgtaacg attaaagtct tatattcttt tgcttttaaa tcttctactg ttgccaagtt
     1261 tagatagtca ttgtctatct tgttgcgcct gcatattaca ctgattgaga ttgcgccagg
     1321 gctctgccca gcagtagtaa taattattgg caacccacct ttaggcagag gtacagtttc
     1381 atcagcccca acagcgttgc tcaaagtgag aagagccaaa attacaaata gcagaatggt
     1441 acataaataa gcaacaacct tcatcttttt gtttaaaaaa tggttaataa acattgttgt
     1501 actcctttta ttaattttat ttattcaaac atattgttaa agtattgtca taattttgat
     1561 aaagaaaatc aaaaatgatt ttttatcagc atttttcttt accctaacca atcagattac
     1621 attttccaaa aggtcatgtt ttgttttaca caaatatgtt ttatccttct ttaaaatacc
     1681 tttctgtgat gatcttatct tcagtaaaga aattaataat ggccttaccc tgtgctttga
     1741 tatcagcaaa ttgagattct ttcattccac caaaaggtaa ataagcaacg ggggcaggaa
     1801 ttccaatatt aattcctatc ataccggctt ccacttctaa tttaaatttt ctggcatagt
     1861 aaccattctg ggtatatata gaagagccat taccatattt atggtcattg ataatttgaa
     1921 tagcctcatc cagattatct gctttcataa tacaaaccac cgggccaaat atttctacat
     1981 catgaatctt cattttaggc tttacatcag taaatacggt gggaccaata aaatatcctt
     2041 tttcacaacc tggtacaaca acacctcttc cgtctaaggc aagggtagca ccctctttta
     2101 ttccctcttc aatcatatgc agaataaaat ccttagcttt tttagatatg actgggccca
     2161 ttaccagagt ttcatttatg aatttggggt caagggggtc tgcaacaata atatttttcg
     2221 aatcttcgat aaagcgctca cacagggttt catacatatc tgctacacca ataataactg
     2281 aagaagccat ac
//
LOCUS       DAOQWT010000259         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_53167_length_2289_cov_7.849597, whole genome shotgun sequence.
ACCESSION   DAOQWT010000259 DAOQWT010000000
VERSION     DAOQWT010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53167_length_2289_cov_7.849597"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1020)
                     /locus_tag="PKJ95_05585"
     CDS             complement(166..1020)
                     /locus_tag="PKJ95_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNR65745.1"
                     /translation="MSIDSSINLRNDVIFKAIFGYEKNEKLLISLLNAILGLTGNKKI
                     VSLTFLNAFNIKEYLKDKLTILDVKAVDGTGVRFDVEMQVAPDKSYLQRIMYYLDRLF
                     TEQLKEGVPYEKLCKAISISILDFIFFEEEGDLHNIYRFLNIKSKRELTDLKELQFIE
                     LPKFKKDKPKRDMSKFEKWLLALKFGEIYANDLENLPEELKEEEEIVMALHELVRASN
                     DDMIREILEMRSKARHDEASRLYQAEMKGKEDVAKNLLEMDMPIEKIAKATGISVEEI
                     EKLKQIRN"
     gene            complement(1102..1947)
                     /locus_tag="PKJ95_05590"
     CDS             complement(1102..1947)
                     /locus_tag="PKJ95_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65746.1"
                     /translation="MAELTTWSVKMTDTLKEKISGSLQESGLTGKEFMEVLLNTYEQV
                     KKDRPEITPDLDELETITKRICAIYVNVGERITTLLNEKDNSYKAIVEEKTDLARTLQ
                     KRITELENAIKERITVAKELTAERDKIQEEKINLELRQQKELQQLAEINQGNKTLIEE
                     YRQKNDTLTGLLAEYETYKSQIQELKKELDQERNKSKEAQGKTKDAEETLKRTKERLF
                     QIEERYKAQIENIIDQHSFEKEKALLEQEKSLRNELQAVRDEYNNRIKVLLDEIEGKH
                     KIVKK"
BASE COUNT          629 a          428 c          325 g          907 t
ORIGIN      
        1 cattagtggt atcgaatagg aaaggtaatg gtggaaatgg ctaaaataaa ggagattatt
       61 gataatgata tagatgataa attgaagaga tttgatgtaa taacgaacta ttctcttgta
      121 gataaaatta agtttggata tattagaata atcagggcat taacttcaat tacgtatttg
      181 ttttagtttt tctatttctt ctactgatat acctgtagct ttagctattt tttctatagg
      241 catatccatt tctaaaagat tttttgcaac gtcttctttg cctttcatct cagcctgata
      301 taatctggaa gcttcatcat gacgtgcttt tgatctcatc tcaagtatct cccttatcat
      361 gtcatcatta gaagctctta ctaactcgtg taaagccatg acgatctcct cctcttcttt
      421 gagctcttcc ggtaaatttt ccaaatcatt tgcataaatc tctccaaact ttaatgcaag
      481 aagccacttt tcaaacttcg acatatcacg tttaggctta tctttcttaa actttggaag
      541 ctctatgaat tgtaattctt tcaaatccgt gagttctctc tttgatttaa tatttaaaaa
      601 cctgtatata ttatgcaagt ctccttcttc ttcaaaaaaa ataaaatcta aaattgatat
      661 gctaatagcc ttacataatt tttcataagg aacgccttcc ttaagctgtt ctgtaaagag
      721 tctgtccaga taatacataa tacgctgtaa atatgattta tcaggtgcaa cttgcatttc
      781 gacatcaaat cttacacctg tgccatctac ggcttttaca tcaaggattg taagtttatc
      841 tttaagatat tcttttatgt taaaagcatt gagaaatgtt aaactaacaa tttttttatt
      901 tcctgttaaa cctaatatag catttaacaa ggatatgaga agcttttcat ttttctcgta
      961 accaaaaatt gccttaaata taacatcgtt tctaagattt attgagctat caatagacac
     1021 ttaacagcct cctttaattc tttctttatt ttaccatata atatattttt taggtatcat
     1081 aacttttctt tcgcagatga tttatttctt tactatctta tgctttcctt caatctcatc
     1141 aagcaaaacc tttatcctgt tgttatactc atctctcacc gcctggagtt cattccttaa
     1201 ggatttctcc tgctccagaa gggccttttc tttctcaaag gagtgctgat ctattatgtt
     1261 ctctatctgt gccttgtaac gctcctctat ctgaaataat ctctctttgg ttctcttcag
     1321 ggtttcttct gcgtctttgg tcttcccctg ggcttcttta cttttattcc tctcctggtc
     1381 cagttctttc tttagttcct ggatctggct tttgtatgtt tcatattctg caaggagtcc
     1441 tgttaaagta tcatttttct gcctgtattc ttctatcaag gttttattac cctggtttat
     1501 ttctgcaagc tgctgaagct ctttctgctg ccgcagctcc agattaatct tctcctcctg
     1561 gatcttatct ctttctgcag taagttcttt agcaacagtt attctctctt taatagcatt
     1621 ttcaagctct gttattctct tctgtaaggt gcgggctaaa tcggtttttt cttcaacaat
     1681 agctttataa gaattgtctt tctcatttaa cagggttgtt attctctctc caacatttac
     1741 gtatatggca cagattcttt tagtaatggt ttccagttca tccagatccg gcgttatctc
     1801 cggcctgtct tttttcacct gctcataggt atttaacagg acttccatga attctttgcc
     1861 tgtaagcccg ctttcctgca ggctgcctga tattttctct ttcaaagtat ctgtcatttt
     1921 aactgaccag gttgttagct ctgccatatt gataacctcc ttataaatag tttactattt
     1981 tattaagtaa accttagtat ttacaatatt ctatagttta ctttaaaatc cttctttgtt
     2041 ttaaaataat taatttttat aagtgtcata tataaggttt attttgcttg tttttcaggt
     2101 ttgcttgcct gaaatatcac cggagataag aacaattaag gatcgcccaa aaagtgaaaa
     2161 atgtttaatt atacaggaat tttatttgca aaaaatgtta taaaaaatca agtaaaccat
     2221 tgcaattatt aggctatttt gatataatta taagtataaa ggaggtgtaa aattgaatat
     2281 aagaataag
//
LOCUS       DAOQWT010000260         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_53944_length_2268_cov_3.072752, whole genome shotgun sequence.
ACCESSION   DAOQWT010000260 DAOQWT010000000
VERSION     DAOQWT010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53944_length_2268_cov_3.072752"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            475..1566
                     /locus_tag="PKJ95_05595"
     CDS             475..1566
                     /locus_tag="PKJ95_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNR65747.1"
                     /translation="MRKKLFIPGPIEVKADVLKEMSRPLIGHRSKEASDLQRGISSNL
                     QKLMYTNNEILLSTSSGSGLMEGAVRSCTNKRAAIFSCGAFGERWYDMALANQVRADL
                     FEVEYGNPIGGQMVKKALDCGKYDLVTITHNETSTGVMNPLPEIADVLRGYPEVIFCV
                     DAVSSLGGVKIAVDELGIDICITSSQKCIGLPPGLSLCSFSQKAVEAARGVKYRGVYF
                     DLLGLYEYKIKRDFQYPFTPSLSHMFALDSQLDSIIKEGLDNRFRRHKMMAERVQRWA
                     KDNFALFPQEQFASPTVTCIKNTRNLNIAGLNRELGEVGFVISNGYGKLENKTFRIAH
                     MGDMTLEEIEGLLSVIDNLLSSGGIQIYA"
     gene            1559..>2268
                     /locus_tag="PKJ95_05600"
     CDS             1559..>2268
                     /locus_tag="PKJ95_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR65748.1"
                     /translation="MLKILIKDEIDPLVVEELKKRKFLVQEGQNGKEVFFKQVQQNEI
                     LIVRSATRVTKELIDSALETGLLKVIIRAGVGVDNIDVSYARSRGIEVFNTPEASSES
                     VAELALAYIFALARKLVSASLTMRQGEWNKKKFLGMELAGKTLGIIGMGRIGQILAKK
                     ASALGMRIIYCDIRTLEQIDSDWRSLPFSEVLKQSDFISLHASSEGNNGYLIDEPQFR
                     MMKKNAFLINTARGRLVN"
BASE COUNT          741 a          326 c          517 g          684 t
ORIGIN      
        1 atcaagataa ttaataaggt gacattatca taaattaatc acaaaaaaat caaaatatat
       61 attgacagct ggactattaa gtgatagtgt aactattaat tttagtatca atcgtttaaa
      121 caaaataagt tctttaattt aaaataaaaa attttataaa taagatataa gatttattaa
      181 attcagaaaa gtaagtctag attctttttc agagagctga gcgttggtga aaaatcagca
      241 aagagtctgc tgaattccgc ctgaaatatt gtcagagaga ttgtcagttt gtttctgacc
      301 gtataaatcg ttagtaaaaa taagagctat tcaagttaga ttattttcca ttatttttta
      361 agatattatg ttgactagct gaattggggt ggcaccacgg catatttatt cgtccccggt
      421 agttgttcaa actaccgggg attttttata aatgaggaaa gtgtggtaga agttatgagg
      481 aaaaaattat ttatccctgg ccctattgag gtcaaagcag atgttttaaa agagatgtca
      541 agacctttga ttggtcatag aagcaaggaa gcatctgatt tgcaaagggg aataagtagc
      601 aatttacaaa aattaatgta taccaataat gaaattctgc tatctacttc ttccggtagc
      661 ggattaatgg aaggagcagt ccgttcctgt actaataaac gtgcagccat tttttcatgt
      721 ggagcatttg gcgagagatg gtatgatatg gctttagcca atcaggtaag ggcagatctc
      781 tttgaggtag aatatggtaa cccaataggt ggtcagatgg ttaaaaaagc tctggattgt
      841 ggaaagtatg atttagtcac tatcacacat aatgagacat ccactggggt aatgaacccc
      901 ttacctgaaa tagctgatgt tttaagagga tatcctgaag taatcttttg tgtagatgcg
      961 gtgagctctt taggtggcgt aaagattgca gttgacgagc tgggcataga tatatgtatt
     1021 acctccagtc agaaatgtat cggtttaccg ccgggtttat cattatgctc tttctcgcag
     1081 aaagcggtgg aggcagcaag aggtgttaag tataggggtg tatattttga tttgttaggg
     1141 ttatatgaat ataagataaa aagggatttt caatatcctt ttacaccttc tctctcacac
     1201 atgtttgctc tggatagcca gctggactcc ataattaaag aaggtctgga caatcgtttt
     1261 agaagacata agatgatggc agagagagtg caaagatggg ctaaagataa ctttgctcta
     1321 tttccccaag aacagtttgc ttccccaact gtaacctgta ttaagaatac tcgtaattta
     1381 aatatagccg gattaaacag agaacttggt gaggtcggtt ttgtaatatc caatggatat
     1441 ggcaaattgg aaaataagac ttttcgtatt gctcatatgg gcgatatgac tttagaagag
     1501 atagaggggt tattatcagt aattgataat ctgttaagct caggaggtat acaaatttat
     1561 gcttaagata ttaattaaag atgagataga tcctttggtg gtagaggaat tgaagaaaag
     1621 aaagttttta gttcaagaag gacaaaatgg gaaagaagtg ttctttaagc aagtacagca
     1681 gaacgagata ctcatagttc gctcagcaac aagagtcact aaggaattga tagattctgc
     1741 tttggaaacg ggtctactca aggtaatcat tagagcaggg gtgggagttg ataatattga
     1801 tgtaagttat gcccgaagca gaggcataga ggtcttcaat acacctgaag ccagtagcga
     1861 gtctgtggca gagctcgccc tggcatatat ctttgcatta gctcgcaagc tggtttcagc
     1921 aagcctaact atgcggcagg gagagtggaa taagaaaaaa tttttaggca tggaattagc
     1981 tgggaaaaca ctgggcatta taggaatggg acgtattggt caaatcctgg ctaaaaaagc
     2041 cagtgctctg ggtatgagaa ttatctattg tgatattagg acactggagc aaatagatag
     2101 tgactggcga tctcttcctt tctctgaagt tttaaaacag tcggatttta tttcgctaca
     2161 tgcctcctct gagggaaata acggttattt gatagatgag ccacaattta ggatgatgaa
     2221 aaaaaatgct ttcctgatta atactgccag gggtaggcta gttaatga
//
LOCUS       DAOQWT010000261         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_54485_length_2254_cov_3.494316, whole genome shotgun sequence.
ACCESSION   DAOQWT010000261 DAOQWT010000000
VERSION     DAOQWT010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54485_length_2254_cov_3.494316"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1182
                     /locus_tag="PKJ95_05605"
     CDS             238..1182
                     /locus_tag="PKJ95_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HNR65749.1"
                     /translation="MQTNLDCIPCFYKQVLRAAKLSGADLKTQREVLIQLSELIPELS
                     LDTSPAEMGRAIYAMISKVTGKKDPYKEIKEESNKIALRIYPEMKTKVMESENQLLTA
                     LQLSIIGNLIGFGLKDFQKISLKIDKIIDNNFYLQDSNFREYFNYQSFCQRLSEVQSV
                     FYLADSAGEVVFDLLFIEQLVTNYDKNVIYVVKGKPVLIDALVDDAIYCGINNFAKII
                     SSGADCPGVVLKYCSPEFIRLFNEAEMIISKGQGNYGALSEEKNPLFFLLIAKCPIIA
                     KHIGCNIGDFVLENNFKEHHWSMEDSESKKQDRKDESQ"
     gene            1169..1633
                     /locus_tag="PKJ95_05610"
     CDS             1169..1633
                     /locus_tag="PKJ95_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNR65750.1"
                     /translation="MKVNDKLTKLILQANGSTYLLLIYVCKFHHLLVGKLGRVKFDPG
                     YYIYLGSAKKSIQKRLLRHLKVQKNKFWHIDYLLSSLTSVKVVNIWVSKKSCECAISQ
                     EILKSGIAVIIQKGFGSSDCRCPAHLFKVNFSDLESLKQLITKKDFYPLSEL"
     gene            1813..>2254
                     /locus_tag="PKJ95_05615"
     CDS             1813..>2254
                     /locus_tag="PKJ95_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNR65751.1"
                     /translation="MAFAIYEEQFRYFLKRNNLKFTKERREVIAAIAIQRNHFNAEDI
                     HQQLKKQKSNVSLATVYRTIPLLIGSGLMTETTYGGERIIYEKIYNKPHHHHMVCINC
                     GKIIDFTCPDVEVVQNDICQKNYFLSTEYRLEIKGYCQTCRKKMK"
BASE COUNT          777 a          355 c          397 g          725 t
ORIGIN      
        1 ttcttactaa tgctaaaaat cagcaacctt aaaccaaaat tatttcacta aaaactttaa
       61 accgaaaact tgtttaatgg caacaactgc actacagtac aacccagagg atagtttttc
      121 tgtctggtaa atacaccaaa ccggtaaacc gtccccatgt ctgtcaaata acaagagtca
      181 aaattgtttt tttgcggtat acgttacact taaaatatag gagttaccaa taaaaaaatg
      241 cagaccaact tagattgtat cccctgtttt tataaacaag tcctgagagc agcaaaatta
      301 tctggtgctg atttgaaaac acagagagaa gttttaattc aattatctga attgataccg
      361 gaactatccc tggatacctc accggcggaa atgggaaggg ccatttatgc tatgatcagt
      421 aaagtaaccg gcaaaaaaga cccctacaaa gaaattaaag aagaaagcaa taagatagcc
      481 ttaagaatat atcctgagat gaagacgaaa gtgatggagt cggaaaacca gctcctgact
      541 gctttacaac tgtccattat tgggaatttg ataggttttg gtttaaaaga ttttcaaaag
      601 atttcactta aaattgataa aatcattgat aataattttt atttacagga cagtaatttt
      661 agggagtatt ttaattacca gtctttctgc cagagattgt cagaagtgca atctgtcttt
      721 tatcttgctg atagcgccgg agaggtagta tttgaccttt tattcattga acaacttgtt
      781 accaattacg ataagaatgt aatctatgtg gtaaaaggta agccagtcct tattgatgct
      841 ctggtagatg atgcaatata ttgtggaata aacaattttg ccaaaataat ctcttctggt
      901 gctgattgtc ccggtgttgt tttaaagtat tgttctcctg agtttatccg tctttttaat
      961 gaggctgaga tgattatcag taaaggtcag ggcaattatg gtgccttgtc agaggaaaag
     1021 aatcccctat tttttttatt aattgccaaa tgtccaatca ttgctaaaca tattggttgc
     1081 aatattggtg attttgtttt agaaaacaat ttcaaagaac accattggtc aatggaagac
     1141 agcgaaagta aaaagcaaga tagaaaagat gaaagtcaat gataaactaa ctaaattaat
     1201 tttgcaggca aatggctcta cctatcttct attaatttat gtgtgtaaat tccaccactt
     1261 gttggttggt aaattaggcc gggttaaatt tgacccgggt tactatatat atttaggctc
     1321 agcaaaaaaa tcaatccaga agcgtctctt aagacattta aaagtacaaa aaaataaatt
     1381 ctggcatatc gattatcttc ttagctctct tacctcagtt aaagtagtta atatttgggt
     1441 tagcaaaaaa tcttgcgaat gtgctattag tcaggaaata ttgaagagcg gaattgctgt
     1501 tataatacaa aaaggttttg gttcttctga ttgtcgctgc cctgcacatc tttttaaagt
     1561 aaatttttct gatttagaat cactgaagca attaataaca aaaaaggatt tttatccctt
     1621 atcagaattg taaaattaca gttaattatt tttctttgct tttcagcccc agcccttggt
     1681 catcatgact cttccaattt ttaggtaata cttgagtatt taatccataa ttattactat
     1741 ttctattgac agaaggatat tgtttgataa aatgtaattg agaatgatat tcaattacaa
     1801 ttggagtata tcttggcttt tgcaatatat gaagaacaat tcagatattt tttaaagagg
     1861 aataatttaa agtttactaa agagagaaga gaagttattg ctgcaattgc aatacagcga
     1921 aatcatttca atgccgagga tattcatcaa caacttaaaa agcaaaaatc caatgtttct
     1981 cttgctaccg tctacagaac tattcccctc ttaattggca gtggtttaat gacagagact
     2041 acctatggtg gagaaagaat aatctacgag aagatatata acaaaccgca tcatcatcat
     2101 atggtctgca ttaactgtgg gaagattatt gactttacct gtccggatgt agaggttgtg
     2161 cagaatgata tatgtcagaa gaattatttt ctctccacag aatatcgttt agaaataaaa
     2221 ggatattgtc agacttgccg aaaaaaaatg aaga
//
LOCUS       DAOQWT010000262         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_54633_length_2250_cov_3.273804, whole genome shotgun sequence.
ACCESSION   DAOQWT010000262 DAOQWT010000000
VERSION     DAOQWT010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54633_length_2250_cov_3.273804"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      458..628
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            737..1321
                     /locus_tag="PKJ95_05620"
     CDS             737..1321
                     /locus_tag="PKJ95_05620"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNR65752.1"
                     /translation="MTTRNIAYYSIFIALAVISGYLIHFPILPQAPFLLYDPGHVFLL
                     IAAFKFGPQAGTIMTLVYALVFALITGQGGPYGALMNFLSTSAFVIVSSWIYLKKHDR
                     NGAIIGLFSGTIAMTGIMIPANLVITPLYLAVNRDIVWKLILPAIIPFNLLKGLISSV
                     FALLVYKKISFLLNKTDYSFSVTHPQTPREQDRS"
     gene            1487..>2250
                     /locus_tag="PKJ95_05625"
     CDS             1487..>2250
                     /locus_tag="PKJ95_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HNR65753.1"
                     /translation="MINRLKGTGKKVLIIIPVLLAILLIAFIGRFYITLIWLKAVGYV
                     SVFWRILIAQFWTGLFFTALFFLLSYFNFNYARRYIPTVQLGSIEPDESRPEMQFYKS
                     LQGFQLSNKIVLGFSAAISLFMGISESVNWEKILIFLNQVSMGIDDPVFGKDIGFYLF
                     SLPFLEYFRNWLSFAIGLIMLIVVLVYFVKRTIRFDYHKIHIDPPVKFHISLLVGAYL
                     FLQAAYFWINSRKILYSTQGAVYGAGYTDIHVSLLA"
BASE COUNT          677 a          375 c          396 g          802 t
ORIGIN      
        1 tgcctctttc tttagaaatt cggatggatg gttattcaga tttctaccac tggttagatc
       61 aatgatggta acctgatgag ttgcttcagt caattttaat attgttccac ccatgcctga
      121 ttctacctgg tgtggatgaa ctccaaaagc gatgattaca gccattattt ataatttcct
      181 ttaattgata tgtgattaaa tatatatgta tttataacat aatattacaa ctgtttaagt
      241 atttgaagaa aaagaactta atgttggaaa gttcttcaac aaaggatgaa ctgctctata
      301 cattttattt ggtaatcata aatataccct tttcctttta atttgtaaat aaaagaagat
      361 taattaatct atttatccaa aacaattttt ttcctaaact tccttgactc tttgttgtat
      421 aattaagtag aaaaatagaa tatagactct taattaaatg cttcttcggg gcagggtgag
      481 gcaattattc aataattgcc aattcccgat cggtggtaat ttgtttttaa tcaaggaaag
      541 attagaaaca aagagcccac gcgtctattc tgactgatcc ggtgtaatcc cggagccgac
      601 agtaaagtct ggaagaaaga agaagattat tgaagacagc cccgaagaga ttcgtgggct
      661 ttttttattt taaataggat tatcaattct aaaatttaac atttattaat atcaattagt
      721 tgaaaggaaa acaaaaatga ccaccagaaa tattgcttac tattctatct ttattgctct
      781 ggctgtgatt tcaggctatt taattcattt tcccatttta ccccaggctc cctttctatt
      841 gtatgacccg ggacatgttt ttttactgat tgcagctttt aaatttggac cccaggcagg
      901 aacaattatg actctggtct atgcactggt atttgccttg attaccggac agggtggacc
      961 ctatggagcc ctgatgaact ttctttccac cagtgctttt gtaatagtct cttcctggat
     1021 ttatttaaaa aagcatgacc gaaacggtgc tattattggg ttgttctcag gcacaattgc
     1081 tatgactggt attatgattc cagcaaattt ggttattacc ccactatatt tagctgtaaa
     1141 tcgggatatc gtctggaaac ttattttgcc ggccataatc ccctttaatc ttttaaaagg
     1201 gctcatcagc agtgtgtttg cccttttagt ttataaaaaa atatcctttc tattgaacaa
     1261 gaccgattac agttttagtg tgacacatcc tcaaacaccc cgggagcaag acagaagctg
     1321 agcattataa ttatagggat agactccttt accatcccac tattttacta aaaaacataa
     1381 ttttaaatta actatttaag atgctataat tggaatgaga gaatgaattt taccgaacaa
     1441 caaaaaaatt gctggtaata ctttttaaga ggagatagaa aaaaatttga taaacagatt
     1501 gaaaggtacc ggaaagaagg tattgataat tataccggtt ttactagcca tcttgctgat
     1561 tgcttttatt ggcagatttt atataacact catctggtta aaagcagttg gctatgtttc
     1621 tgttttttgg aggatattaa ttgcccaatt ttggacggga ctttttttca ctgccttatt
     1681 ctttctgcta agttatttca attttaatta tgctcgtcgt tatatcccca ctgttcagtt
     1741 agggtcaatt gaacctgatg agagcagacc ggaaatgcaa ttctataaaa gcctgcaggg
     1801 ttttcagttg agtaataaaa ttgttttagg attttcagca gctatctctt tattcatggg
     1861 tatttctgaa tcagttaatt gggaaaagat attaatattt ctcaatcagg tgtctatggg
     1921 tatagatgac cctgtttttg gaaaagatat tggtttctac ctgttcagcc tacctttctt
     1981 agaatatttt agaaattggc tgagttttgc tattggcctt attatgttaa ttgttgtatt
     2041 ggtatatttt gtgaaaagaa ctattcgatt tgattatcat aaaatacaca tcgatcctcc
     2101 cgtaaaattc catatctcac tactggtggg agcctattta ttcttacagg cagcatattt
     2161 ctggattaac tcccgtaaaa tattatattc tacacaggga gcagtctatg gagctggtta
     2221 tactgacatt catgtaagtc tgctggcttt
//
LOCUS       DAOQWT010000263         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_54753_length_2247_cov_2.938412, whole genome shotgun sequence.
ACCESSION   DAOQWT010000263 DAOQWT010000000
VERSION     DAOQWT010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54753_length_2247_cov_2.938412"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PKJ95_05630"
     CDS             <1..800
                     /locus_tag="PKJ95_05630"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNR65754.1"
                     /translation="EELLKQEAGSKKPIYEFESDDGMKNCCWQIQEKEIIISLIKAFQ
                     DIPSLYIADGHHRTAAAVEVAQLLKAQQKKGEDKEKKVIEEYMYFPAVLIPDNQIHIS
                     AYHRVVRIPLDFNKDYFINQLAQIFKIEKISPNHPFLPVKKHEFGMNIFGQWYRLLVK
                     EGILHKEKRDITSRLDVSILQELVLYPILGIKYPQQNNIIEFIGGVNSLPKIEERIRQ
                     GAVIVFTLFPTLIEDVIEISEMEKIMPPKSTWFTPKLRSGIFVHYFG"
     gene            1038..2036
                     /gene="argF"
                     /locus_tag="PKJ95_05635"
     CDS             1038..2036
                     /gene="argF"
                     /locus_tag="PKJ95_05635"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNR65755.1"
                     /translation="MKVNLRNRHLLTLEDFDKEEIAFLIELAAELKKAKHNGKEVQKL
                     KNKNIALVFEKPSTRTRSAFEVAAHDQGANITYLEAISSHIGLKESMEDTARVLGRYY
                     DGIGFRGFKQESVEILAKYSGAVVWNGLTDTKHPTQAIADLMTIKEKIKKPLEKVIIT
                     YVGDGRANTSNSLLLISSIFGIKFRIVSPKLLYPNRELLDTAKKIAEKSGSSIELTDN
                     ILKGVRGSDVIYTDIWVSMGEEHLIGERILLLKEYQVDMDMIKATENPDVLFLHPLPS
                     FHDINTKIGMEVFHKFGLKSMEVTDEVFRSKHSVVFDEAENKLHTIKAIMIATLGN"
BASE COUNT          816 a          337 c          429 g          665 t
ORIGIN      
        1 tagaagaatt gttgaaacaa gaggctggta gcaaaaaacc aatatatgaa tttgaatctg
       61 atgatggaat gaaaaattgc tgctggcaga ttcaggagaa ggagattatt atttctctga
      121 taaaagcctt tcaggatatt cccagtcttt acattgctga tggacatcat aggactgctg
      181 cggcagtaga ggtagctcaa ttactgaaag ctcaacagaa aaaaggtgaa gataaagaaa
      241 aaaaggtaat cgaagaatac atgtattttc cagcagtgct aataccagat aatcaaattc
      301 atatttctgc ctaccatcga gtagtgagaa tacctcttga ttttaataaa gattatttta
      361 tcaaccagtt agcacagata tttaaaattg aaaagatttc ccccaatcac ccctttttac
      421 cagtcaaaaa acatgaattt ggtatgaata tattcgggca atggtatagg ttattagtaa
      481 aagagggaat tttacataaa gaaaaaaggg atattaccag tagactggat gtatctatct
      541 tacaggagct ggtattgtat cccatattgg gaattaaata tcctcaacaa aacaatataa
      601 ttgaatttat aggtggagtt aattccttgc caaaaatcga agaaagaatc aggcaaggag
      661 ccgtcattgt atttactctg ttccctacct taattgaaga tgttatagag atttctgaaa
      721 tggaaaaaat tatgccaccc aagtcgacct ggtttacacc aaaactgaga agtggtattt
      781 tcgtccacta ttttggctag aaatttagaa agacgttctc tgctgtgaga gaatttactt
      841 taccataact catactaagg gtatactatt atatagaagt acagaattga actgtgaaaa
      901 tttaaaagtt gttattcatt attattaaga catggggaag actaactttt atttatttcc
      961 tatacctgtt caatgcctat ttactgaaca ataagattaa aatctggttt tacagaagat
     1021 agaagaaagg gaaaaagatg aaagtcaatt taagaaatcg tcatttattg actcttgagg
     1081 attttgacaa agaagaaata gcttttttaa tagaactggc tgcagaactt aaaaaagcta
     1141 aacataatgg aaaagaggtg caaaaattaa agaataaaaa cattgcatta gtatttgaaa
     1201 aaccatcaac caggaccaga agtgcttttg aggtggctgc acatgatcag ggagccaata
     1261 ttacctatct ggaagcaatt agttctcaca taggcttgaa ggaatctatg gaggatactg
     1321 ccagggtttt aggccgttat tatgatggga taggatttcg gggatttaaa caagaatctg
     1381 tggagatatt ggctaaatat tccggagcag tggtctggaa tggcctaaca gacacaaaac
     1441 atcctacaca ggctatagct gacttaatga ccatcaaaga aaagataaaa aaacccctgg
     1501 aaaaggttat aatcacatat gttggggatg gtagggcaaa tacctctaac tccttacttt
     1561 taatttcttc aatttttggt attaaatttc gtattgtatc cccaaaactt ctctatccca
     1621 atagagaatt gctggatacc gcaaaaaaaa ttgcagaaaa atctggtagc agcattgagc
     1681 taactgataa tattttaaaa ggggtacgcg gtagtgatgt gatttatact gatatatggg
     1741 tgtcaatggg agaagaacac ttaatcggag aacggatatt attgctaaaa gaataccagg
     1801 tggatatgga tatgattaaa gctacagaaa atcccgatgt cttattttta catcctttac
     1861 cttcttttca tgatataaat accaaaatag gaatggaggt ttttcataag tttggattga
     1921 agtctatgga agtaactgat gaagtatttc gaagcaaaca ttctgtggtt tttgatgaag
     1981 cagaaaataa gcttcatacc ataaaggcga ttatgatagc taccttaggt aattgatact
     2041 atctttggta tcagtacaaa ccataagttt taattgatac cttataaata tagttcaatg
     2101 aaaaaggagt ttaatcaaaa tgaaaaagtt tatcttcatt aaaactgaaa tccctggacc
     2161 gaaatcaaaa gaaatcgctt taagtagaaa ccagtttgta gcaaaaccaa tgagcagttc
     2221 tcttaccccc agctttattg cccatgg
//
LOCUS       DAOQWT010000264         2243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_54908_length_2243_cov_3.302102, whole genome shotgun sequence.
ACCESSION   DAOQWT010000264 DAOQWT010000000
VERSION     DAOQWT010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54908_length_2243_cov_3.302102"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1736
                     /locus_tag="PKJ95_05640"
     CDS             <1..1736
                     /locus_tag="PKJ95_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR65756.1"
                     /translation="FDSPACVEVINEEQIFNTKAENLASILKNIAGVEITDYGTPGDI
                     KAISIRGSSPEQVLVMIDGQVANGVQTGKIDLGLIPADMIEKIEIYRGSASALYGANA
                     LGGVINIITKKGGKKEGTLSIHYGNYHTQKYNVSYQDRIDDYTYFLTGEYFKTEGSKE
                     NSQLEKLSFMSRVSKEIDSQTELDLIIRFNDHQRGIPGSLDFPSPNAWQNDRTFSMNI
                     NWQKTEEDRDYNIVAWYDFHKLSYDNPDEWGYDGTSIHKNNSSGISFDTTYYDFTFGN
                     KKESAYTHTLTWGGDISYNWTDSTDIGKHQNLQGAVFIQDAWQIKEKLSATAGLRYDY
                     NQLFAGQLNPRIGLNYRLQDELSLHMSVSRAYRAPNYNDLYWPEDGFVGGNVNLLPEI
                     AWAYEAGMRFINKKGDTQAELNLFRKNVKDLINWAADSDGIWRPSNIGTARVDGLEFI
                     TRKDFNNHLTANLNYTFLNAIDLESGTQLKPKHKYGIGLTYSDQFGKNNDDFVINLDG
                     YLVAGRPDNQEAYYLFDINAARDFSLGKDKNKKVNLSFSVKNIFNQKPELVSGYPIQG
                     RTYLLGISTRF"
     gene            1797..>2243
                     /locus_tag="PKJ95_05645"
     CDS             1797..>2243
                     /locus_tag="PKJ95_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65757.1"
                     /translation="MENRFFIFALIISLIFHYWAIMFFSDIATSWKYLENKNEIIVSA
                     SIDYFMPPSLGQPVEEEFDTPYLNKESALAETNRSEEIELPEEIFEKEELDKKEISVT
                     EQYLSETEEILIDEMPSHNDTINERDESGSNYVKDQANKTHDLILVK"
BASE COUNT          784 a          376 c          432 g          651 t
ORIGIN      
        1 tatttgattc acctgcctgt gtagaggtaa taaatgaaga gcaaattttt aataccaaag
       61 cagagaattt agcatctatt ttaaaaaata ttgccggagt agaaataaca gattatggca
      121 ctccaggtga tattaaagca ataagtatta gaggctcctc tcccgagcag gtcctggtaa
      181 tgattgacgg acaggtagcc aatggtgtac agaccggtaa aattgacctt ggtttaatac
      241 cagcagatat gatagaaaag attgaaatat atcgtggatc agcctctgct ctctatggag
      301 caaatgccct gggcggtgta attaatatta ttactaaaaa gggtggtaaa aaagaaggaa
      361 cattaagcat acattatggt aattaccata ctcagaaata caatgtttca taccaggaca
      421 gaattgatga ctatacctat tttttaacag gagagtattt taaaactgaa ggaagcaaag
      481 agaacagtca attagaaaaa ttatctttta tgagtagagt ttctaaagaa attgactctc
      541 aaactgagct ggatttaatt atccgtttca atgaccatca gagaggcata ccaggttcgc
      601 ttgatttccc ctctcctaat gcctggcaaa atgaccgtac ttttagtatg aatataaact
      661 ggcaaaaaac agaggaagac agagattaca atatagtggc ctggtatgat tttcataaac
      721 tctcttatga caacccggat gaatggggat atgatggtac ctctatacat aaaaataata
      781 gtagcggaat ctcttttgac accacttatt acgatttcac ttttggaaac aagaaagaat
      841 ctgcctatac tcatacctta acctggggtg gtgatataag ttataactgg acagacagta
      901 ccgatattgg taaacatcaa aatctgcagg gtgctgtctt tattcaggat gcctggcaaa
      961 taaaagaaaa actttctgcc actgctgggc ttcgttatga ttacaaccaa ttatttgcag
     1021 gacaattaaa ccccagaatc ggcttaaact acagactgca ggacgaactt agccttcata
     1081 tgtctgttag ccgtgcctat agggcaccta attataatga cctttactgg cctgaagatg
     1141 gctttgtagg aggaaatgtt aatttgctac cggaaatagc atgggcttat gaggcgggaa
     1201 tgcgctttat aaataaaaaa ggtgatactc aagctgagct gaatctattt agaaaaaatg
     1261 taaaagacct gatcaattgg gctgctgatt ctgatggaat atggcggcca tccaatattg
     1321 gaactgcaag agtagatggc ttagagttca tcacacgaaa agactttaat aatcacctga
     1381 ctgctaactt aaactatact tttttaaatg ctatagacct tgaatcaggc actcaattaa
     1441 aaccaaaaca caaatatgga attggtttaa cttattcaga ccaatttggg aaaaataatg
     1501 atgattttgt aattaacctg gatggctacc tggttgctgg cagacctgac aatcaagaag
     1561 cttattatct ctttgatata aatgcagcaa gagatttttc attaggaaag gataaaaata
     1621 aaaaggttaa tttaagtttt tctgtcaaaa acatattcaa tcagaaaccg gagctggtta
     1681 gcggctatcc gattcagggt agaacatatc tactgggtat cagtaccaga ttttaaataa
     1741 aaataagagt gggatggtct atcttttttg gactatccct ctccttcttt aagagaatgg
     1801 aaaatagatt ttttatcttt gctttaataa tttctttaat atttcattat tgggctatta
     1861 tgttcttttc tgatattgca actagctgga aatatctgga aaataaaaat gaaataattg
     1921 tctcagccag tatagattat tttatgccac cttctctcgg tcagcccgtc gaagaagagt
     1981 ttgacacacc atacttaaat aaagaatcag ctcttgctga aacaaaccga tcagaagaga
     2041 ttgagttacc agaggaaatt tttgaaaaag aagaacttga taagaaagag atttccgtta
     2101 ccgaacaata cttatcagaa acagaagaaa ttcttataga cgaaatgcct tcccataacg
     2161 atacaattaa tgagcgggat gaatctggaa gcaattacgt taaagaccaa gcaaacaaaa
     2221 cccatgacct gattttggtt aaa
//
LOCUS       DAOQWT010000265         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_54956_length_2242_cov_2.987654, whole genome shotgun sequence.
ACCESSION   DAOQWT010000265 DAOQWT010000000
VERSION     DAOQWT010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54956_length_2242_cov_2.987654"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /gene="eno"
                     /locus_tag="PKJ95_05650"
     CDS             <1..567
                     /gene="eno"
                     /locus_tag="PKJ95_05650"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889699.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNR65758.1"
                     /translation="YIALDAAASEFYKEGKYILSREGVERTAEEMIAYYEELVKKFPI
                     ISIEDGLAEDDWENWSKLTKSLGNKIQIVGDDLYVTNQTRLYKGIQLGASNSILIKLN
                     QIGTLTETLDTIEMAKRAGFSCIISHRSGETEDTIIADLAVATNAGQIKSGSLCRTDR
                     IAKYNQLLRLEEELGEQAVYRGKGVFKR"
     gene            567..854
                     /locus_tag="PKJ95_05655"
     CDS             567..854
                     /locus_tag="PKJ95_05655"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNR65759.1"
                     /translation="MSYARSLFESKITWAIILILIFYITFIFSEKYARILEMQSYLAE
                     LEYEIDDLKRDTELLSEKIDLLNTNQYIEKIAREELDLVKPNEIMFKSIKK"
     gene            1090..1175
                     /locus_tag="PKJ95_05660"
     tRNA            1090..1175
                     /locus_tag="PKJ95_05660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1124..1126,aa:Leu,seq:taa)
     gene            1292..1368
                     /locus_tag="PKJ95_05665"
     tRNA            1292..1368
                     /locus_tag="PKJ95_05665"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Met,seq:cat)
     gene            1476..1552
                     /locus_tag="PKJ95_05670"
     tRNA            1476..1552
                     /locus_tag="PKJ95_05670"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1510..1512,aa:Met,seq:cat)
     gene            1635..>2242
                     /gene="tilS"
                     /locus_tag="PKJ95_05675"
     CDS             1635..>2242
                     /gene="tilS"
                     /locus_tag="PKJ95_05675"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNR65760.1"
                     /translation="MDKLIRKVLKTIEKYSMIEKGDMIILAISGGPDSTCLLDILNQI
                     KEQFKISLMVAHVHHGIRKKEADVEARFVRLKSFHLGLPFQQKFLSVPRMAKEKGLSI
                     EQAGRRARYEFFLELADKYQAQKIALGHHADDQIETILMRLIRGSGLRGLRGIPPKRG
                     VFIRPLIECTREEIEAYCKRRKLTFCFDSTNKEPMYLRNKIR"
BASE COUNT          749 a          366 c          461 g          666 t
ORIGIN      
        1 tatattgctt tagatgcggc agcctctgaa ttttataaag agggaaaata tattttaagc
       61 agagaaggtg tggagaggac agcagaagaa atgattgctt attatgagga gctggtaaaa
      121 aaattcccca taatttccat agaggatggg ttagcagaag atgattggga gaattggagt
      181 aaattaacca agagtctggg aaataaaata cagatagtag gggatgacct ctatgtaacc
      241 aatcaaaccc gcctgtacaa gggaattcag ttaggagcat ctaattccat tcttattaag
      301 ctaaatcaaa ttggcaccct gactgaaaca ttggatacca ttgaaatggc aaaacgagct
      361 ggttttagct gtatcatctc acatcgctcc ggagaaactg aagatactat tattgcagat
      421 ttggcggtgg caaccaatgc cggtcagatt aaatcgggtt ctctttgccg gactgaccga
      481 attgcaaaat acaatcaatt gttaagatta gaagaagagt tgggagagca ggcagtatat
      541 agaggaaaag gagtttttaa aagataatgt cttatgccag atctcttttt gaatctaaaa
      601 taacctgggc aataatctta atattaatct tttatatcac ctttattttt tcagaaaaat
      661 atgcacgcat tctggaaatg caatcatatc tagcagaatt ggaatatgag attgatgatc
      721 ttaaaaggga tactgagctg ctttctgaaa agatagactt attgaatacc aatcaatata
      781 ttgagaaaat agcgcgggaa gaattagatc tggtgaaacc caatgaaatt atgtttaaat
      841 ccataaaaaa atagttgaat gggattatca caccttcctt cagcagaatc acaataataa
      901 aagcacttag acggtgtgca ttgaattcaa tgtcttggcc cagaagtgtt gccaatgatg
      961 ataaggtcct ttttactaat aagcgtaact agtagctaaa tggtatattc taaccggctg
     1021 atactctaca tattattttt agaactatat tgacgactat ggtcttttaa tctataataa
     1081 tctaagtttg ccggagtggc ggaataggta gacgcacagg acttaaaatc ctggaagctg
     1141 caaggcttgt gccggttcga ttccggcctc cggcacctgt tatgatgatt gctgaattac
     1201 aaaattaatt tagcagcaat tgactttaat atcctatagt gatagtatta ataggcaatt
     1261 taaaaaataa taaataagta cctataaaaa tagcggggtg gagcagctag gtagctcgtc
     1321 aggctcataa cctgaaggtc ggcggttcga atccgtcccc cgcaaccatt tttattcaat
     1381 taaacctctg ttgtttaaaa gctgttacca gaggtttaat taatattaag taagttattt
     1441 ttctttcatt ttttgtctgc ttaaattttt ttattggcgg tgtagctcag ctggctagag
     1501 catgcggttc atacccgcag tgtcctgagt tcgaatctca gcaccgccac caaactccag
     1561 gacctaataa aaaatttcaa atactttaac tattaccatt cccaaaaaaa ttttatagat
     1621 atagaatgtg ataattggat aaattaataa gaaaagtact taaaacaatt gaaaaatata
     1681 gcatgataga aaaaggagat atgattatcc ttgccatttc agggggcccc gattctacct
     1741 gcctgcttga tattttaaat cagattaaag aacagtttaa aatttcttta atggtagccc
     1801 atgtccacca tggtatcaga aaaaaagagg cagatgttga ggcgcgtttt gttcgtctga
     1861 aatctttcca tttaggtctt ccctttcagc agaaatttct atctgttccc cggatggcaa
     1921 aggagaaggg gctctcaatt gaacaggcgg gcaggagagc acgttatgaa ttttttctgg
     1981 aattagctga taaataccag gctcagaaaa ttgctttagg ccatcatgca gatgatcaga
     2041 tagagactat tttgatgagg cttattcggg gtagtggatt gagagggtta aggggcattc
     2101 cacctaaaag aggagttttt ataagaccgt tgattgaatg tacccgtgaa gaaatcgaag
     2161 cctattgcaa aagaagaaag ttaacttttt gttttgattc taccaacaaa gaaccaatgt
     2221 atttacgtaa taaaattaga ca
//
LOCUS       DAOQWT010000266         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_55428_length_2230_cov_2.657011, whole genome shotgun sequence.
ACCESSION   DAOQWT010000266 DAOQWT010000000
VERSION     DAOQWT010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55428_length_2230_cov_2.657011"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PKJ95_05680"
     CDS             <1..687
                     /locus_tag="PKJ95_05680"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNR65761.1"
                     /translation="EGKLIYTELMAVSGETVGQQISKAENKNTEVIRKFDNPYSSESG
                     IAILYGNLAPEGAVVKASAVSKRMFIFEGKARVFNSEEEAYQAIMDGKIQKGEVVVIR
                     YEGPKGGPGMREMLAPTSALCGMGLDNNVALITDGRFSGATRGAAIGHISPEAAQKGP
                     IGLIEEGDIIKIDIGKRELSVKLSEDELKQREANWSPPERDIPSTYYLKRYAYLVTSA
                     STGAVFRNID"
     gene            942..1478
                     /locus_tag="PKJ95_05685"
     CDS             942..1478
                     /locus_tag="PKJ95_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNR65762.1"
                     /translation="MIPLLKKRRSIRLFQDRKVEKEKIYQIMQAALLAPSSKDNNPWK
                     FIIVEDRETLIKLSEAKEHGSNLLVKAPLAIVVLADPSHSDVWIEDSSIATTLIILEA
                     QQLGLGSCWIQLRRRYHASREDSEIYLRNLLGIPKSLNILCMVAIGYPDETKPEKTIP
                     EQKFNDIFLNRYNQKYNF"
     gene            1521..>2230
                     /locus_tag="PKJ95_05690"
     CDS             1521..>2230
                     /locus_tag="PKJ95_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNR65763.1"
                     /translation="MKEEFGLVLEGGGAKGAYEIGACKALEEMGMNITAVTGTSVGAL
                     NGAMVAQHEIEKAYQLWYNIHPSQVMNIEAEVLDKLARLEIRPSDIRYYLKILGEIIT
                     EGGIDVSPLQKLIKKYVNEEKLRNSDIIFGMVTVSFTDRKALELFIEDIPDGQIADYL
                     FASANFPLFRSAKIGDKVYLDGGIYDNLPIRMLINRGYTSLIIVQVGGLGRKRKVNLN
                     HLNVINIETKEYLGGPLS"
BASE COUNT          778 a          355 c          489 g          608 t
ORIGIN      
        1 gagggcaaat tgatatacac agagttgatg gccgttagcg gcgagacggt aggtcaacaa
       61 atatctaaag cagaaaataa aaatacagag gtaattagaa aatttgataa cccttatagt
      121 tcggaaagtg gtattgctat tctgtatggt aacctggcac ctgaaggtgc ggtagtaaaa
      181 gcctctgctg tcagcaagag aatgtttatc tttgaaggca aagcccgggt atttaattca
      241 gaggaagaag cttatcaggc tataatggat ggtaaaattc aaaaaggaga ggtagttgtt
      301 atccgttatg agggacctaa agggggacct ggaatgagag aaatgctggc accaacctct
      361 gctctttgtg gaatgggatt ggacaataat gttgccctga ttacagatgg ccgtttttca
      421 ggtgcaacac gtggtgcggc aattggccat atttctccgg aagctgctca aaaagggcct
      481 attggtctga ttgaagaggg ggatatcatt aaaatcgata ttggcaagag agaattgtca
      541 gtgaagttaa gtgaagatga gctaaaacag cgtgaggcaa actggtcacc accagaaaga
      601 gacatcccct ctacatatta tctgaagaga tatgcctacc tggtaacatc tgccagcaca
      661 ggtgccgtct ttagaaatat agattaggca gccacaaatc atagtgaaaa gtgattagta
      721 ataagtgata agtaaacaat agtgagtaat gagtgaataa taaagataga aaaaaagatt
      781 tttgtatttg actaaaaact atcgactaac gaccacttac atttataccg gcaatgatta
      841 taactaacaa ccaacgactt atacaataca aatatctaat taactaacaa agaatatcta
      901 atgtttcgtg atataatata ataaagggag cttgattaaa aatgatacca ttgcttaaaa
      961 aaagaaggag tattcgttta tttcaggata gaaaagtgga gaaagaaaaa atatatcaga
     1021 ttatgcaggc tgctctttta gcaccatcat caaaggataa caatccctgg aaatttatta
     1081 ttgttgaaga tagggaaacc ctgataaaac tatctgaagc gaaagaacat ggttctaact
     1141 tactggtaaa agcgcccctg gccattgtgg tactggctga tcccagccac tcagatgtat
     1201 ggatagaaga ttcctccatt gctactactt taattatact ggaagcacag caattgggtc
     1261 tgggtagttg ttggatacag ctgagaagaa gataccacgc aagcagagaa gattcggaaa
     1321 tatatctcag gaacttgttg ggaataccaa aaagtttaaa catcttatgt atggttgcta
     1381 ttgggtatcc agatgaaaca aagcctgaaa aaacaattcc ggaacaaaaa tttaatgata
     1441 tttttctaaa ccgctataac caaaaataca atttttaaac ctatacaaat attgagcaat
     1501 aaaacaaaaa aggtgctcat atgaaagaag aattcggatt agtattagag ggtggaggcg
     1561 cgaaaggtgc ttatgaaatt ggcgcctgca aagctttaga agagatggga atgaacatta
     1621 ctgcagttac cggaacatct gtgggagctt taaatggtgc tatggtagcc cagcatgaaa
     1681 tagagaaggc ttatcaactc tggtacaata tccatccttc acaggttatg aatattgaag
     1741 cagaagtatt agataaatta gcccgtttag aaataagacc gagtgatatc cgttactatt
     1801 taaagatact gggggaaatt attactgaag gtggtattga tgtctcccct ttacagaaac
     1861 taatcaaaaa atatgtcaat gaggaaaaac tgcgcaactc agatattatc tttgggatgg
     1921 tcacggtatc ttttactgac cgcaaggcgc tggaattatt tatagaggat attcctgatg
     1981 gccagattgc tgattatctt tttgccagtg ccaatttccc tctttttcgt tcagctaaaa
     2041 ttggtgataa ggtttattta gatggtggaa tttatgataa tttacccatt cgtatgctaa
     2101 ttaatcgcgg ttatacctct ctcattatcg tacaggtcgg agggctgggg agaaagagaa
     2161 aagtaaattt aaaccatctc aacgtcatca atattgaaac aaaagaatat ctgggagggc
     2221 cattaagttt
//
LOCUS       DAOQWT010000267         2221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_55752_length_2221_cov_3.563250, whole genome shotgun sequence.
ACCESSION   DAOQWT010000267 DAOQWT010000000
VERSION     DAOQWT010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55752_length_2221_cov_3.563250"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PKJ95_05695"
     CDS             complement(<1..1052)
                     /locus_tag="PKJ95_05695"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNR65764.1"
                     /translation="MVGYRAKRRKQGSKSFLGRSFLISIILNILFILAFNNLISFDFL
                     NIPEIEEMIMVTLVELPSIQKPTTVKPEITREEPIAEVSVRPRVEPVLPEPAKTQEIE
                     TQPKIEKKEVQIESGPRVEVKMPEIDIPAKIEMAPVKEEVSIRSDMQVAARELSPSIS
                     SRREIEGEVLERGEFEIQAKLGLEGRERVESTYGTVVTPGELSNMPRAVEKESPFGKR
                     PLAIMIDNAKDSRPQSGLEKANVVYEVLAEGGITRFLGIFASQDADKVGPVRSARPYF
                     ITKTLEHNAIYVHAGESPDAAIFIKEERIDDINELVHFQPFWRTSDRKPPHNLYTSTE
                     RLREEAKRLGYIEMVN"
     gene            complement(1093..1506)
                     /locus_tag="PKJ95_05700"
     CDS             complement(1093..1506)
                     /locus_tag="PKJ95_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNR65765.1"
                     /translation="MKFYHPNSKTLKFDLTPLIDVVFQLLIFFMLTTTFINVESGVKV
                     DLPTGDFAAVSEKRNVVVTITENNVLYLNNRLVDPNYLIEKLREETKDSPNPLVVLEA
                     DQNVTHGKVVRVMDLIKKSGIERIAIATKPREEGF"
     gene            complement(1481..2137)
                     /locus_tag="PKJ95_05705"
     CDS             complement(1481..2137)
                     /locus_tag="PKJ95_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNR65766.1"
                     /translation="MFELFYKGGILMYPIFFCSLLAIAIFFERMFFLKSIKTSTRKFG
                     NRISDLIRKGNINFAITACRKNYSPISQIILAALLKYGSSREEIKEAIEDSANQEVAI
                     LEKNLPILATVGNIAPLLGLLGTVFGMIKGFQVISAIGVGNPEALAGAISEALLTTAF
                     GLSVAIPTIVAYNYLVNRVERQIKEMEATSVEILELLSVKRGINEEDIKIDEILSPQQ
                     "
BASE COUNT          656 a          471 c          318 g          776 t
ORIGIN      
        1 ttattaacca tttcaatata ccccagtctc ttcgcctctt ccctcagcct ctcagttgag
       61 gtataaaggt tatgcggagg ttttcgatcc gatgttctcc aaaaaggttg gaaatgaacc
      121 aattcattaa tatcatcaat tctttcttct ttaataaata ttgctgcatc aggactttct
      181 ccagcatgca catatatggc gttatgctct aatgttttag taatgaaata gggtctggca
      241 ctacgaacag gaccgacttt atcagcatcc tgtgaggcaa aaatacctaa aaaacgggtg
      301 atcccgcctt ctgcaagtac ttcatataca acatttgctt tttctaatcc actttgaggt
      361 cgtgaatctt tggcattatc tatcattata gccaggggcc tcttgccaaa aggagattct
      421 ttttctaccg ccctgggcat attacttaat tcaccaggag ttaccactgt cccataggta
      481 gattcaactc gttctcttcc ttccaggcct aactttgctt gaatttcaaa ttcgcctctt
      541 tctaatactt ccccttcaat ttctcttcta ctggaaatac ttgggcttaa ttctcgagcc
      601 gctacttgca tatcacttct aattgatact tcctctttaa ctggtgccat ttctattttg
      661 gcaggaatat caatttctgg catttttact tccaccctgg gaccactttc aatttgaact
      721 tccttctttt ctattttcgg ttgagtctca atttcctgag tttttgcagg ttcaggtaat
      781 acaggttcta ctctcggtct aacacttact tctgcaattg gttcctctct ggtaatctca
      841 ggtttaacag tagttggttt ttgaattgaa ggtaattcta ccagagtgac cattatcatc
      901 tcttcaattt ctggaatatt taaaaaatca aaagagatta agttattaaa agccagaatg
      961 aataagatat ttaatattat tgaaattaaa aatgaccgcc ctaaaaaact ttttgaacct
     1021 tgttttcttc tttttgctct gtatcctacc attattttcc ttactcactt caatttatta
     1081 atttatatct ctttagaacc cttcttctct cggtttagta gctatggcaa tcctttcaat
     1141 tccacttttt ttgatcagat ccataactct aactacttta ccatgagtaa cattttggtc
     1201 ggcttctaaa acaactaaag gatttggact atctttagtc tcttctctca acttttcaat
     1261 taaataattg gggtcaacca aacggttgtt aagatacaat acattattct ccgtaatagt
     1321 aaccactaca tttctttttt cgcttacagc agcaaaatct ccagtgggta agtcaacctt
     1381 gaccccactt tcaacattta taaaagtagt agtaagcata aagaaaatta gcaattgaaa
     1441 tactacgtct attaagggag ttaggtcaaa tttaagagtt ttactgttgg ggtgataaaa
     1501 tttcatctat ctttatatcc tcctcattaa ttcctctttt aacagacaat aattctaaaa
     1561 tctcaacact agttgcttcc atttctttta tctgtctttc caccctgttt acaagataat
     1621 tataagcaac tatagtagga atggccacgg aaagaccaaa agcggtagtc aatagagcct
     1681 ctgaaatcgc tccagccagg gcctctggat ttcccactcc aattgctgag attacctgaa
     1741 atccttttat cataccaaaa acggtaccca atagccctaa taagggagcg atattcccca
     1801 cagtagccag tattggtaaa tttttctcta aaatcgctac ttcttgatta gcagaatctt
     1861 ctattgcttc tttaatttct tcccgagaac tgccatactt taaaagtgcg gccaatatta
     1921 tttgtgatat gggagaataa ttcttacgac aggcagtaat agcaaaatta atatttcctt
     1981 ttctaatcaa atcactgata cggttgccaa attttcgagt agatgttttg atactcttaa
     2041 ggaaaaacat tctttcaaaa aaaatagcga tagcaagcaa ggaacagaaa aatatggggt
     2101 acattaaaat accgccttta taaaataatt caaacattaa tcagttgcct cctccaatgt
     2161 tataaaatat ctttatcttt atatcacatc attaattatc aatactcaat cacatcaata
     2221 c
//
LOCUS       DAOQWT010000268         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_55964_length_2216_cov_2.302175, whole genome shotgun sequence.
ACCESSION   DAOQWT010000268 DAOQWT010000000
VERSION     DAOQWT010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55964_length_2216_cov_2.302175"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKJ95_05710"
     CDS             <1..453
                     /locus_tag="PKJ95_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNR65767.1"
                     /translation="KDLAMMAVNYGNAYVARVALAADMNQAIRVFREAEAYNGPSLIV
                     AYSHCVAHGYDLRNGIKQQKAAVASGYWPLMHYNPGLRKEGQNPFVLDSKAPSIPLEE
                     YIYAENRYRMLTYSAPEIAKEYYKKAQQEVTNRWKIYEKWAKDDNSQK"
     gene            502..1497
                     /locus_tag="PKJ95_05715"
     CDS             502..1497
                     /locus_tag="PKJ95_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNR65768.1"
                     /translation="MDLSTEYLGIKLKNPLVASSSPLCEDIENIRLMEKSGISAVVLH
                     SLFEEQVILQEKELNYALTENTERYAESLSYFPEVDDYHFAPEEYIKYIDKVKKAVQI
                     PVIGSLNGVSNTGWIRYSKKIEEAGADGLELNIYYLPTRVDLTCQELEKMYLDLVQEV
                     RQTIKIPLAVKLGPYFNSFVHFCDKLVGAGVDGLILFNRFYQPDVDLLEMSVSPNLEL
                     SSSSELRLRLRWIGILYNKIKADLAVTGGIHTAKDILKSIMVGAKVTMMTSALLEQGI
                     PYVETLVKGTEEWLSRNNYQSIDELRGILTEGNVKETDAFERANYMKVLQSYHYE"
BASE COUNT          733 a          329 c          521 g          633 t
ORIGIN      
        1 aaagatctgg ctatgatggc agttaattat ggaaatgcct atgtggcaag agttgccctg
       61 gctgctgata tgaatcaggc tataagggta ttccgtgaag ctgaagccta taatggccca
      121 tctctcattg ttgcctattc acactgtgtg gcacacggct atgatctgag aaacggtatt
      181 aagcagcaga aagcggcagt agcctccgga tattggccct taatgcacta taacccgggg
      241 ttgagaaaag aagggcagaa tccttttgtg ctggatagca aagctcccag tatcccatta
      301 gaagaatata tctatgcaga aaacagatat agaatgctaa cttatagcgc acccgaaatt
      361 gccaaagagt actataaaaa agcccagcag gaagtaacga atcgctggaa gatatatgaa
      421 aaatgggcta aagatgataa ttcccaaaaa taaaataaat aaaatataga ttatagattc
      481 aaattggggg ataagaaaat gatagattta agtactgaat atttaggtat taaattaaag
      541 aatcccttgg ttgcctcttc ctctcctttg tgtgaggata ttgaaaacat cagacttatg
      601 gaaaaaagcg gaataagtgc tgtagtttta cactcactct ttgaggaaca ggttatcctg
      661 caggaaaaag aattgaacta tgcccttacc gaaaacacgg agagatatgc tgaatcattg
      721 agctattttc ctgaagttga tgattatcac tttgctcctg aagaatatat aaaatatatt
      781 gataaggtaa aaaaggctgt gcagataccg gttatcggta gtctaaacgg cgtatctaat
      841 accgggtgga ttcgttacag caaaaagatt gaagaagcag gagctgatgg gctggaactg
      901 aatatctact atttacccac cagggttgac cttacctgtc aggagttaga gaaaatgtat
      961 cttgacctgg tacaggaggt aagacagact ataaaaatac ctctggctgt aaaattaggc
     1021 ccttatttca attcctttgt gcatttttgt gataaattgg ttggtgcagg ggttgatgga
     1081 ttaatcttat ttaatcgctt ttatcagccg gatgttgatc tattggaaat gtcggttagc
     1141 cctaatctgg aattgagtag ctcatctgaa ttgcgattgc gtttgagatg gataggaatt
     1201 ctctataata agattaaggc agatctggca gtcactggag gaatacatac ggccaaagat
     1261 attctgaaaa gcataatggt aggagccaaa gtcaccatga tgacctcagc actgttggag
     1321 caaggaattc cttatgttga gacgctcgta aaaggcactg aggaatggtt aagtcgaaat
     1381 aattatcagt ctattgatga gttgaggggc atattaacag aaggtaatgt gaaagagact
     1441 gatgcctttg aaagggctaa ttatatgaag gtgttacagt cttaccatta tgaataaaaa
     1501 acagttaaac aggggacggt tcactgttta actcccaagg actttgtaaa acagaaaacc
     1561 gtccccattt ttgcctgttt agttattagt cgttggtcga tagtatatag tatttagtga
     1621 agtgggtata agtgaaagag gttttttaat aaagatgcaa agaaaacccc cttctgtcat
     1681 tgcgagcgac cggagggagc gaagcaatct catccagtta tgctgggaga attgaaagca
     1741 aatgaagttt ttggtttatg gttcttggtt tttggaaaaa acctaagaaa atacaaagag
     1801 agtggaaata agtgagggct taaaagataa agagtgcggg caacagagta tgagattgcc
     1861 acacactgat ttcacagtgt tcgcaatgac attgcagtgg gagtaaatgg tggtataaag
     1921 tttttggtta aaaagatgtg ggagtagatg aaaggggttt tttgttcata gtttatggat
     1981 tttggtaaaa agcaaagagg aagagcgcgg ggagaagtat caaacagggg acggctcact
     2041 gtttaacttc taaggacttt gtaaaacaga aaaccatccc tatttctgcc tgtttagtcg
     2101 ttaatcgatg atatttggtt attaaagttt ttagtaaaat actaaaataa agatgtgagt
     2161 agaagtgaag gaatagtgtt tttagcttat aaacagtttt tattcgttat aatatt
//
LOCUS       DAOQWT010000269         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_56032_length_2214_cov_2.971746, whole genome shotgun sequence.
ACCESSION   DAOQWT010000269 DAOQWT010000000
VERSION     DAOQWT010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56032_length_2214_cov_2.971746"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="PKJ95_05720"
     CDS             <1..1390
                     /locus_tag="PKJ95_05720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNR65769.1"
                     /translation="FTTNLGDAVRQSQVIFIAVGTPSNNDGSADLNQVEEVAKEIARN
                     LNGYKIIVNKCTVPVGTARKIKQIIEENQILNNTSKVNPGDLSAYNSCKDSFVLSGSD
                     DEPSSYKYCKNSSSNNNCQQPPISSHSENYSIPCQFEDHSPSSIYQDPFSLRHCEEQS
                     DEAISSTSTHIPFDIVSNPEFLREGNAVYDFTHPDKIVIGYESEKAKNVMQEIYRPLY
                     LLNTPFVFTTLETAEMIKYACNAFLATKITFINEIANLCDKVGADVHEIARAMGMDGR
                     ISPKFLHPGPGFGGSCFPKDTRALMATAHSNNYDFKLLNAVIEANLRQRKIVVDKIEE
                     GLGILKDKTIAILGLAFKQNTDDIRESPAHDVIKILLEKDVNIKCFDPMAMENTKKIF
                     PNLTYCQNEYEAVKDADALVILTEWNQFRNLDLIKIKKLLKSPILFDMRNLYEPFKVK
                     EIGIKYFGIGRR"
     gene            1558..>2214
                     /locus_tag="PKJ95_05725"
     CDS             1558..>2214
                     /locus_tag="PKJ95_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65770.1"
                     /translation="MNQKSFLKKLYEIFTPRERMQAVMVFFASLATAFVQAFGVASIF
                     PFINVVMNPDIVGQNKWLSLLYNKLNFNDINSFILFLGIAVFLIVILSGTISAITTWA
                     KTRFTLGKNHSLSRRLLTVYLSQPYEYFLQKNSSELSKNILSEVNQLTSQLLMALFDI
                     LINGLMIVVMVTMLFMVNITVTMGAIIFLGGSYSLINYFVKNKLRRVGRERLEANKGR
                     F"
BASE COUNT          746 a          412 c          378 g          678 t
ORIGIN      
        1 ctttactacc aatcttggag atgcagtaag acaatctcag gtgattttca tagcggtagg
       61 tacgccttcc aataatgatg gttctgcaga tttaaaccag gtagaagagg tagccaaaga
      121 gattgcccga aatttaaatg ggtacaaaat cattgtcaat aaatgtactg ttccggtagg
      181 tacagctcgc aaaatcaaac aaatcatcga ggaaaatcaa atattaaaca ataccagcaa
      241 ggtaaatccg ggagaccttt ctgcttataa ctcttgtaag gattcctttg tcttatctgg
      301 ctctgacgat gaaccctcgt cttataaata ctgcaaaaat agcagttcta ataataattg
      361 tcagcagcca cctatatcaa gccattccga gaattattct attccctgtc aattcgaaga
      421 ccattccccc tccagcattt accaggaccc attttccctt cgtcattgcg aggaacaaag
      481 tgacgaagca atctcatcca cttctaccca catccctttt gatattgtct ccaatcctga
      541 attcctccgg gaaggcaacg ctgtctacga ttttactcat cctgataaaa tagtcatagg
      601 atatgaatca gaaaaagcaa aaaatgttat gcaggagatt taccgtcccc tctatcttct
      661 taatactcct tttgtcttta ccactttaga gacagcggag atgattaaat atgcctgcaa
      721 tgcctttctg gcaaccaaaa tcactttcat caacgaaata gccaacctct gtgacaaagt
      781 aggagcagat gttcatgaga ttgccagggc tatgggtatg gatggtcgta tcagcccaaa
      841 attcctgcat cctggtcccg gttttggtgg ttcttgcttc cctaaagata ccagagcctt
      901 aatggccact gcacatagca ataattacga ttttaaattg ttaaatgccg tcattgaagc
      961 aaatctacgg caaagaaaga tagtagttga caagatagaa gaaggtctgg gcattttaaa
     1021 agataaaaca attgccattt taggactggc atttaaacag aatactgatg atatcagaga
     1081 atcccctgct cacgatgtta taaagatttt acttgaaaaa gatgtaaata taaaatgttt
     1141 tgaccctatg gcaatggaga acactaaaaa aatttttcca aaccttactt attgtcaaaa
     1201 tgaatatgaa gcagtaaaag atgcagatgc tttggttatc cttactgaat ggaaccaatt
     1261 tcgtaatctt gatttaataa aaataaaaaa attgttaaag tcgcctatac ttttcgatat
     1321 gcgtaacctc tatgagcctt ttaaagtaaa agaaataggc attaaatatt ttggaattgg
     1381 cagaaggtga accaattaac taatttacta attaactaat tgatcaattc attaattgcc
     1441 tgatttgcta attacctaat taaccaacta actaattaac caactaacca atgagctaat
     1501 taactaatta accaattaac taattaacaa actaattaac caattaacaa attaaccatg
     1561 aatcaaaaat cttttctaaa aaaactatat gaaatattta ctcccagaga gcgtatgcag
     1621 gcagtgatgg ttttctttgc cagtttggct actgcctttg tacaggcttt tggtgtggca
     1681 agtatctttc catttataaa tgtagtaatg aatcctgata tagtagggca gaacaaatgg
     1741 ctttctctgc tctataacaa acttaatttc aatgatataa attctttcat tctattttta
     1801 gggattgccg tttttctgat agtaatactt tccggcacta tctctgcaat aactacctgg
     1861 gcgaaaacaa ggttcacgct gggaaaaaac cactcgttat ctcgcagact cttaactgtt
     1921 tatttgagcc aaccctatga atatttcttg caaaaaaaca gcagcgaatt aagtaaaaac
     1981 atattatctg aagtaaacca actcaccagc cagcttttaa tggctttatt tgatatttta
     2041 attaatggtt tgatgattgt ggttatggtt actatgttat ttatggttaa tataacagta
     2101 acaatgggtg ccataatctt tttagggggt agttatagct taataaacta ttttgttaaa
     2161 aacaaattgc gtagagtagg aagggaaagg ttggaagcga ataagggaag attc
//
LOCUS       DAOQWT010000270         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_56443_length_2204_cov_3.062820, whole genome shotgun sequence.
ACCESSION   DAOQWT010000270 DAOQWT010000000
VERSION     DAOQWT010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56443_length_2204_cov_3.062820"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PKJ95_05730"
     CDS             <1..756
                     /locus_tag="PKJ95_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR65771.1"
                     /translation="KIFYKGTDILSLTRKQFRKYRREIQMIFQDPYSSLDPRQTIGKT
                     IGEPLKVNKVKGDIDKKVKTLMKEVGLLEEFYNRYPHEFSGGQRQRIGVARALALDPT
                     LIVCDEPVSALDVSIQAQIINLMQDLQEKFQLTYLFISHDMSVVKHMCDRVAVMYLGK
                     LVELAEKDKLFETPKHPYTKVLLKSIPVPDPDKKKEQEAAMGDVPSSIDLPPGCRFQS
                     RCKECKDVCKFVEPELLEIEPGHFVRCYLYNSK"
     gene            966..2057
                     /gene="buk"
                     /locus_tag="PKJ95_05735"
     CDS             966..2057
                     /gene="buk"
                     /locus_tag="PKJ95_05735"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNR65772.1"
                     /translation="MNEIIFTVNPGSTTTKCALFNLTEQRVTKVIEEQIEHPEKEIAQ
                     FPAISDQINYRETIAYNFIDKYLPENANIIACAGRGGMLTPVPSGAIKINEELVKFSL
                     YTPVYQHASNLGAPLAFRIAQRYGAPAFIVDPVSVDEFSAVARISGHPDFPRFSFVHA
                     LNIRATVRKFAEQIGKDFSDTCCVAAHLGGGFSIAAIDHGKIVDNDNKMESAPFTPER
                     AGGIPPIPLIEACFSGRYKKEELVRKLYGEGGVYAYLGSKNMVELEKRAEKGDKEVRR
                     IYYAMAYQIAKEIAAMASVLNFEIDGIIITGGLAHDQFLIKELKKKIKKLGDIYLYPG
                     SNENEALAETVARVLAGKESYIDWPVRVS"
BASE COUNT          760 a          323 c          457 g          664 t
ORIGIN      
        1 aaaatatttt ataaaggtac agatatatta agtttaacca ggaaacagtt tagaaaatat
       61 agaagagaaa ttcagatgat ttttcaagac ccttattctt ctttagatcc cagacaaact
      121 ataggaaaga ctataggaga accattaaaa gtaaataaag taaaagggga tatagataaa
      181 aaagtaaaga cattgatgaa agaagttggt ttactagagg agttttacaa cagatatccc
      241 catgaattta gcgggggaca acgtcaaagg ataggagttg ctcgggcttt agcccttgat
      301 cctacactta ttgtatgtga tgaaccagtt tcagcactgg atgtttctat tcaggctcaa
      361 attattaatc ttatgcagga tttacaagaa aaatttcaat taacttattt gtttatttct
      421 cacgatatga gtgttgttaa acatatgtgt gacagggttg ctgttatgta tttaggaaaa
      481 ttggtagaat tggctgaaaa agataaacta tttgaaaccc ctaaacaccc ttataccaag
      541 gtattgttaa aatcaattcc tgttcctgac ccagataaga agaaggaaca ggaagctgcg
      601 atgggagatg ttcccagctc aattgatttg cctccagggt gtagattcca atctaggtgc
      661 aaggaatgca aggatgtatg caaatttgtt gaaccagaat tgttagaaat tgaaccagga
      721 cattttgtga gatgttattt atataacagt aaataatcta taaaaatatt acaactatta
      781 tttcctaacc cggaaggcat gaatagcaaa tttattaaaa aaattttttt tcaataaatt
      841 ttagattgat tatattgtta tacagtataa actacgtata tattcataat tgaatttcgt
      901 gcagcctgaa ataaaaaacg catacataaa gttttatata ttaaaacaaa taagaaagat
      961 aatctatgaa tgaaataatt tttacggtga atcctggttc cactactacc aaatgtgctc
     1021 tattcaatct aactgaacaa agggttacga aagtgattga agagcaaatt gaacatcccg
     1081 aaaaagaaat agctcaattt cctgcaattt ctgatcaaat taattaccgg gaaactattg
     1141 cctacaattt tattgataaa tatttacctg agaatgcaaa tattattgcc tgtgccgggc
     1201 ggggaggaat gttaactccg gtgcccagtg gagccataaa aattaatgaa gaactggtta
     1261 aattttcttt atatacacca gtctaccagc atgcttccaa tttaggtgct cccttagcct
     1321 ttcgaattgc tcaacgctat ggagctcctg cttttatagt agacccggtc agtgttgacg
     1381 aattttctgc tgtggcccgg atttccggac atcctgattt tcccaggttt agttttgttc
     1441 atgccttgaa tataagagca acggtacgaa agtttgctga acaaattggc aaagatttca
     1501 gtgatacttg ttgtgttgct gcccatctgg gaggaggttt ctctattgct gctatagatc
     1561 acggtaagat tgtagacaat gacaataaga tggagtcagc cccctttaca ccggagagag
     1621 ctggaggaat cccacctatt cctttgattg aagcctgttt ttctggaaga tataaaaaag
     1681 aagaattagt gcgtaaatta tatggtgagg gaggggttta cgcctatctg ggaagtaaga
     1741 atatggttga attagaaaaa agggctgaaa aaggggataa agaagtaaga agaatatatt
     1801 atgctatggc ttaccagatt gccaaggaga tagcagccat ggcttctgtt ttaaattttg
     1861 aaattgatgg tattattatt accggtggtc tggctcacga tcaatttcta attaaggaat
     1921 tgaaaaagaa gataaaaaaa ttgggagata tttatttata tcccggtagc aatgagaatg
     1981 aagccctggc agaaacagtt gccagagtat tagctggtaa ggaaagctat atagattggc
     2041 cagtcagggt tagctagtta gaaaggtaag ataatgataa aaaattattt tgaattagaa
     2101 aaagctgcgc agagattgca accggtaaga atggttgtgg tttctgccga agatgaacag
     2161 gttttaaaag gagtaaaatt agctttagac aaggggatta tctg
//
LOCUS       DAOQWT010000271         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_56452_length_2204_cov_2.520242, whole genome shotgun sequence.
ACCESSION   DAOQWT010000271 DAOQWT010000000
VERSION     DAOQWT010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56452_length_2204_cov_2.520242"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PKJ95_05740"
     CDS             complement(<1..311)
                     /locus_tag="PKJ95_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65773.1"
                     /translation="MQSNKPGAIIIEGHVQGLSNTRSLGEAGIPVYVVDKIKCIARHS
                     KYCRKFFLCPDFIQDEFATFLIDLAEKEGVKGWVLIPSNDHAVYTISKHKTRLEQYYK
                     L"
     gene            complement(298..2040)
                     /locus_tag="PKJ95_05745"
     CDS             complement(298..2040)
                     /locus_tag="PKJ95_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNR65774.1"
                     /translation="MKRENKPQIPEQDISIVLCGEAGQGIQTVEELLTRILKLSGYYV
                     FATKEYMSRVRGGSNSTQIRVSSKPIQAPVARIDVLIPLDREAIKHVSYYIDEHTIII
                     GDEEKLDTNKKVINVSFLKMAQEIGKAIYANIIAVGLLLGLLDGDIKIGKDFLTDYFT
                     EKNKKEFVEDNIKALEKGYQEARRLIKENLLKSERLPKLKKSSKVKNNLLFNGAEAVG
                     LGAIAGGCNFISSYPMSPSTGVLTFLAQHAHDFDIIAEQTEDEISAINMAIGAWYTGA
                     RAMVTTSGGGFALMTEGLSLAGMIETPVVIHLAQRPAPSTGLPTRTEQGDLLFVLYAG
                     HGEFPRIILAPATIEDAYYLTQKAFNWADKFQIPVIILTDQYTMDTYYNIKKLTIPKE
                     EINNFIIKTDSDYHRYNLKIKDGISPRGIPGFGEGLVRVDSDEHDEMGLITEDMDIRK
                     RMVEKRNAKWYPLKKEILPPTLAGAKDYRILLVGWGSTFNIIKEAMACNCPIVSTDVG
                     DVKWLFGNTEGCYLTSFDPEDVAEKIRSALEFAASKGRTNGRERMIELGLDADTVSQK
                     LVMVYKNVLKDAKQ"
BASE COUNT          617 a          489 c          379 g          719 t
ORIGIN      
        1 attaatttgt aatactgctc aagtcgggtt ttatgttttg aaatagtgta aacggcatga
       61 tcattggaag gaattaaaac ccacccttta acaccttctt tttcagcaag atcgatgagg
      121 aacgttgcaa attcgtcctg aataaaatcc gggcaaagaa aaaacttcct gcaatattta
      181 gaatggcggg caatgcattt tattttatcg accacatata ccggtattcc ggcctcaccc
      241 agcgaacgtg tattcgacag gccctgcaca tgcccttcga tgataatggc gccaggttta
      301 ttgctttgca tcttttaaaa catttttata aaccatgact aatttttggg atacagtgtc
      361 agcatcaagt ccaagctcaa tcatgcgttc ccggccatta gtccttcctt ttgatgctgc
      421 aaattccagg gccgatctta ttttttcagc cacgtcttcc ggatcaaaac tggtcagata
      481 acagccttcc gtgtttccaa aaagccattt cacatcaccg acatcggttg atactatggg
      541 acaattgcag gccattgctt ctttaataat attgaatgtt gacccccatc ctaccaataa
      601 tatacggtaa tcctttgcgc cggctaatgt aggtggtaat atttcttttt ttaagggata
      661 ccatttggca tttctttttt ctaccattct ctttctaatg tccatatctt ctgtgataag
      721 tcccatctca tcatgctcat cactatctac tctgaccagt ccttctccaa aaccaggaat
      781 ccctcttggc gatatcccat cctttatctt tagattatat ctatgataat cagaatcagt
      841 cttaatgata aaattattta tctcctcttt aggaatagtt aactttttaa tattgtaata
      901 ggtatccata gtgtattggt cagttagaat tatgaccggt atctggaatt tatcagccca
      961 attaaatgcc ttctgagtaa gataataggc atcctcaata gtggcaggag caagtatgat
     1021 gcgtggaaac tctccatgtc cggcatagag gacaaatagt aaatcccctt gctcagtcct
     1081 ggttggtaat ccggtagatg gtgctggccg ttgagctaaa tgaataacta caggggtttc
     1141 aatcatacct gccaagctga ggccttcggt catcaaggca aaacctcctc cggaggtagt
     1201 aaccatagca cgtgcaccag tataccaggc tccaatagcc atattaatag cgcttatctc
     1261 atcttctgtc tgttcggcaa taatatcaaa atcatgggca tgctgagcta aaaaggtcaa
     1321 aactccagta gagggagaca ttgggtagga agatataaaa ttacaacccc cggcaattgc
     1381 tcctaaccct actgcctctg cgccattaaa caacagatta ttcttcactt tgctggattt
     1441 tttaagtttt ggcaaacgtt cagattttaa gaggttttct ttaatcaacc tccttgcttc
     1501 ctgatacccc ttttctaaag ctttgatatt atcttctaca aattcttttt tattcttttc
     1561 ggtaaaatag tctgttaaaa agtctttacc aattttaata tctccatcca gtaaacccag
     1621 taaaagtccc actgctatta tattggcata aatggctttc cctatctcct gagccatctt
     1681 taaaaaagat acattaataa ccttcttatt agtatcgagt ttttcctcat caccgataat
     1741 gatggtatgc tcatcaatat agtaagaaac atgttttata gcctctctat ccagtggtat
     1801 caacacatct atacgagcaa ccggggcttg aatcggtttg ctggatactc gaatctgggt
     1861 ggagttactt cctcctctca cccgggacat atattcttta gtggcaaaaa cataataccc
     1921 agatagcttt aaaattctgg tcaataattc ttccactgtt tgtataccct gtccagcttc
     1981 accacaaagc acaatagaaa tatcttgctc tggtatttgc ggcttatttt ctcttttcat
     2041 cccttcctcc ctcctttttt aggaaaattt atctggtaaa tccttttcct tttgcaaaac
     2101 aggtatggca gatgaaatag acatattatt tatgattaga atttttagca ggcggcaagg
     2161 acggtccttt atatggtacc ggaatatact gcactgatgg acgc
//
LOCUS       DAOQWT010000272         2202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_56538_length_2202_cov_2.735911, whole genome shotgun sequence.
ACCESSION   DAOQWT010000272 DAOQWT010000000
VERSION     DAOQWT010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56538_length_2202_cov_2.735911"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1382
                     /locus_tag="PKJ95_05750"
     CDS             174..1382
                     /locus_tag="PKJ95_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNR65775.1"
                     /translation="MENITDTHFHGGKEQHQEYYSRLMMEAVSDGRITQRDSNQIQAF
                     ISEVKACGKLSSQRAYKLSYQLVGLRKWLPEFSQVDTSAVFSGIERLKAESGYTEITQ
                     GDMIKLCKRFLLWLYDSGEAAPGLKPEKLRKIKAKTVSATKTAEDILTPDQIDALFKQ
                     VSNTRNRAMLELLYESAGRAGEIATLVWGQVTFYQNYAAVRLDGKTGKTRQAPLYSSH
                     IALRRWRDQYKGGNPAADDLVFPGREGGDQPMSYTGMAKIVRIAAHEAGIEKPVTLHT
                     FRHSRITHLLQAGMNESTIKMLAWGDVGSNMLKVYSHLTPGDVENSFNQLYGIKPVEE
                     FKGMDPAVSPRQCPACGMLNPPGNNFCGGCSMALTEAARNEKDEAINRIMKDETLRTA
                     LTDLLKQRGL"
     gene            complement(1377..1895)
                     /locus_tag="PKJ95_05755"
     CDS             complement(1377..1895)
                     /locus_tag="PKJ95_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65776.1"
                     /translation="MKPLEEELKIYLNSKYSGNTKKYVNYIVDVLNVPDETGAPALVR
                     AKVLQDALVPGKIPYSGLYSRLLKEMVETGIITKEIGEHEPGKRGKPPVFYRLKSNNL
                     LFLHDTLSSLHKEREELLSQSPEELVNRIGEITIEKLELELRLDIAIKLLQEAGINPD
                     EIEKEVRELTEL"
BASE COUNT          643 a          455 c          540 g          564 t
ORIGIN      
        1 tatcagatat tcacactgtg aagaggaagt ttgtgcattg atgagaaaag aagggtaata
       61 cgtgcccgga tatatttagg gacttaaact ttagttatag agcctcaggc gggctttaca
      121 actaaagttt aaggctccat ttagtttata tactcagccg ggcaattaat ggtatggaga
      181 atatcaccga cacgcatttt catggaggga aggaacagca ccaggaatat tattcccggc
      241 taatgatgga agcggtatct gatggaagga taacacagag ggacagtaac cagatacagg
      301 cgtttatctc tgaagtgaaa gcgtgtggga agctatcaag tcagcgggca tataagctga
      361 gttaccagct ggtagggtta agaaaatggc tccctgaatt ctcacaggtg gatacatcag
      421 ccgtcttctc agggattgaa cgactaaaag cagagagcgg gtatactgaa attactcagg
      481 gagatatgat aaaactctgt aaacggtttc ttctatggct ctatgatagc ggggaagcgg
      541 caccagggct gaaaccggag aagctcagga agataaaggc caaaaccgta tcagcaacta
      601 agacggctga ggatatcctg accccggacc agatagacgc gctattcaaa caggtatcca
      661 atacgagaaa ccgggctatg ttggaacttc tctatgagtc agcaggaaga gcgggggaga
      721 ttgccacact tgtatgggga caggttacct tttatcaaaa ttatgcagcg gtccggctgg
      781 atgggaagac agggaagacc cggcaggcac ccctgtattc ttctcatatt gctttgagac
      841 gatggaggga ccagtataaa gggggtaacc ctgcagcaga tgacctagtt tttcccggaa
      901 gagaaggggg ggaccagccg atgtcataca ctggcatggc aaaaatagta agaattgcag
      961 cacatgaggc cgggatagaa aaacccgtga ccctgcacac cttccggcac tcgagaatta
     1021 cccatttatt acaggcggga atgaatgaaa gcactattaa gatgctagcc tggggggatg
     1081 ttggctcaaa tatgctgaaa gtttattctc atcttacccc tggagacgtt gaaaactcat
     1141 tcaatcaatt gtatggtatt aaaccagtgg aagagtttaa gggaatggac ccggcagtat
     1201 caccccgtca atgtccggcg tgtgggatgc ttaacccacc agggaataat ttttgcgggg
     1261 gctgttcaat ggcattaacc gaagcggcac ggaatgagaa ggatgaggca ataaatagga
     1321 taatgaaaga cgaaaccctg aggacggccc ttactgactt gttgaaacag cggggattat
     1381 aactctgtca gctctcttac ctccttttct atttcgtccg ggttaattcc tgcttcctgt
     1441 aagagtttaa tggcaatatc taaccttaat tccaactcta gtttttctat ggttatttct
     1501 cctattctat ttacaagctc ttcaggtgat tgtgagagta attcttctct ctctttgtgc
     1561 agggatgata aggtatcatg caggaataac aggttattac ttttcaggcg gtaaaatacc
     1621 gggggcttcc ctcttttccc cggctcatgc tctcctattt ccttggtgat aatccccgtt
     1681 tctaccatct ctttgagaag ccgagaatac agcccggaat atggtatctt acctggcacc
     1741 agggcgtctt gtaatacttt agccctgaca agtgcaggcg ccccggtttc atcagggacg
     1801 tttaagacat ctacgatata attaacgtat tttttggtat tcccgctata tttggagttt
     1861 aaataaattt ttaactcttc ttcaagcggt ttcattaatc ctaacgttat acctacaggt
     1921 atttctaaac tttatcctta cgtgagtact cacgtaagga taaatacctg aaacatcata
     1981 tgataattgt cgcacgaaca gcgcgatagg aaaaaaagaa tgaccgtctt gatttacaca
     2041 agaaacggaa aggaagtcgg ataccgtcac acatctgtga ctattccgaa tgagttatat
     2101 caggaagcga agaacaaggg cgtaagccta tcagcggtac tgaccagggc tttagagagc
     2161 ggtgaacatg caaagcagta accacacccc ggagccctgg ag
//
LOCUS       DAOQWT010000273         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_56830_length_2195_cov_2.748598, whole genome shotgun sequence.
ACCESSION   DAOQWT010000273 DAOQWT010000000
VERSION     DAOQWT010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56830_length_2195_cov_2.748598"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /gene="murG"
                     /locus_tag="PKJ95_05760"
     CDS             complement(<1..987)
                     /gene="murG"
                     /locus_tag="PKJ95_05760"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNR65777.1"
                     /translation="MKIIITGGGTGGHIYPGISLAHEFQRRDKNNDIVFVGTEHKMES
                     EIIPREGFRFLGLRVKGIERKLSLESLSALFLFIGSLFVSFRIVKNYKPDLVIGTGGY
                     VSGSVALVSSLLGIPTFIHEQNITPGITNQFLSLTSKKVFTSFPESKKYFWRKSRIIF
                     LGNPIRKNIWQGNKENLIKAHNMDGSKKTILVFGGSKGASSINETVLDSLELIDSSLW
                     LEWQVLIISGKEDYNNITEKIANSQYKDMVRVLDYLYNIEDAYDLANIVVCRAGATTI
                     AELSAKGISAILIPYPYATGNHQLFNALFLENNNAALVIREEELSSEGLAHKL"
     gene            complement(992..2113)
                     /gene="ftsW"
                     /locus_tag="PKJ95_05765"
     CDS             complement(992..2113)
                     /gene="ftsW"
                     /locus_tag="PKJ95_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNR65778.1"
                     /translation="MNLLPGKQPDYFLISIIYVLLSLGIIMVFSSSYIMAYKWYGDSF
                     YFFTRQVFSALIATIVFLITMKIDYQYWRKFATPILIISLFLLIALYLPGVARYIRGA
                     KRWIYIGPFPFQPSELAKVALILYIADCLTRKTDKDIGTFVRGILPVFLVTALIFILI
                     FAQPDFSTGIVVLGCSFIMLFIGGSRITQLFTLITFLTPPGILMVLGKEYRRVRLLSF
                     LNPWEDPLKSGFHIIQSLIALGSGGLSGVGIAQSRQKYFYLPDQHTDFIFSIIGEELG
                     FLGTSLVIILFLIFLWRGFMIAWRAKDTFGALLAAGITSLIVFQAIVNISVVTKVFPT
                     TGITLPFVSYGGSSLIVNMFCCGILLNISKSIKIKGEVI"
BASE COUNT          730 a          445 c          385 g          635 t
ORIGIN      
        1 caatttatga gctaatcctt cactgctaag ctcttcttcc ctgataacca gagcagcatt
       61 gttattttcc aaaaatagtg cattgaagag ctgatgatta ccagtcgcat aaggatatgg
      121 tatcaatatt gccgatattc ctttagctga cagctcggca atggtagtag cacccgctct
      181 gcacaccact atattagcca ggtcataagc atcttctatg ttatacaaat aatccaatac
      241 ccttaccata tctttatatt gggagttagc tatcttttct gtaatgttgt tataatcttc
      301 tttgccactg ataattaata cctgccattc cagccatagt gagctatcta tcagttcgag
      361 actatccaat accgtttcat tgatggaact ggctcccttg ctaccgccaa ataccagtat
      421 ggttttttta gaaccatcca tattatgcgc ttttatcaaa ttttctttgt tgccctgcca
      481 gatatttttt cgaattggat tgcccaggaa gattattcta ctctttctcc aaaaatattt
      541 ttttgattcc ggaaaactgg taaaaacctt tttgctggtt aaagataaga actgattggt
      601 aataccgggg gtaatatttt gttcatgaat aaaagtggga atccccaata gcgaggagac
      661 caatgcaaca gaaccactta cataacctcc ggtccctata accagatcag gtttataatt
      721 ttttactatt ctaaatgaaa caaaaaggga gcctataaag agaaagagtg ctgataaact
      781 ctctaagctt aactttcttt ctatcccttt aacacgtaaa ccgagaaaac gaaatccttc
      841 ccggggaatt atctctgatt ccattttatg ttcagtaccc acaaaaacaa tatcattatt
      901 cttgtctctt ctctggaatt catgtgccag gcttatcccg ggatatatat gtcccccggt
      961 gcctcccccg gtaatgataa ttttcattta atcaaattac ctcgcctttt attttaatac
     1021 ttttagaaat attgagaaga attccacaac agaacatatt aacaattaag gatgaaccac
     1081 cgtaacttac aaaaggaagg gtaattcccg tggtggggaa taccttagtt accacactga
     1141 tattaactat tgcttgaaaa acaattaaag aggtaatacc agctgctaac aatgctccaa
     1201 aggtgtcttt agccctccag gcgatcataa aacctctcca taaaaagata agaaataaaa
     1261 taataaccag ggaggtaccc aaaaatccca gttcttctcc aataatagaa aaaataaaat
     1321 cagtatgctg gtcaggaagg taaaaatatt tttgtctgct ctgggcaatt cccactccgc
     1381 ttaagccgcc gctaccaaga gctatcaaag actgaataat atgaaacccg cttttcaagg
     1441 ggtcttccca ggggtttaaa aaggagagca gtctgactct gcggtattct ttccccagta
     1501 ccataagaat tccaggagga gtgagaaagg taataagggt aaataattga gtaattctgc
     1561 ttcctccaat gaaaagcata ataaaggaac aacccagaac aacaatgccg gtactgaaat
     1621 cgggctgggc gaaaattaag ataaagatta gggcggttac cagaaatacc ggtaaaattc
     1681 cccgaacaaa ggtgccaatg tctttgtccg tttttctggt taagcaatca gcaatataaa
     1741 gaattagggc caccttggct aattccgagg gttgaaaggg aaaaggacca atataaatcc
     1801 acctcttcgc tcctctgata tatctggcaa ctccaggtag atacagtgct atcaataaaa
     1861 agagggatat aatcaaaatt ggtgtggcaa actttctcca gtattgataa tcaatcttca
     1921 tagttattaa gaaaacgatg gtggcaatga gggcagaaaa gacctgtctg gtaaaaaaat
     1981 agaagctatc gccataccac ttataagcca taatataact ggagctaaaa accattatta
     2041 ttcccagaga gagtaaaaca tagataatgg aaatcaagaa atagtctggt tgtttacctg
     2101 gcaataaatt catctgaact gctattccct tctttctttt tctttagaag aatcaattaa
     2161 atggatagga aaataaacct aacagggcaa ataat
//
LOCUS       DAOQWT010000274         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_56862_length_2194_cov_3.772324, whole genome shotgun sequence.
ACCESSION   DAOQWT010000274 DAOQWT010000000
VERSION     DAOQWT010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56862_length_2194_cov_3.772324"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="PKJ95_05770"
     CDS             complement(<1..1419)
                     /locus_tag="PKJ95_05770"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNR65779.1"
                     /translation="MIKSIIHSTEIEVGGKKLKIETGKLAKQANGSVMVTYGETVVLV
                     TAVASKEPKEGTDFFPLLVDYEEKFYAAGKIPGGFFKREGKPSENAILTSRLIDRPIR
                     PLFPEGYYNDVQIIASVLSYDQKNLPDIISIIGASCALYISNIPFYKPIGAIRVGYLN
                     GNFLINPTVEELTECQLNIVVAGTKDSVIMLEGEARELSEGIVLEAIETAQTEIKKIV
                     EMQIGFAQKINPEKNNKSWQLEESLIEERNSFYELIKKDYSEQISEFLITREKAEREE
                     KLENLKEEIITELKNNLSPEEELDVLIINEVYDSLCKDLMRRLIIEKGLRVDHRKPDE
                     IRAIECEIDILPRVHGSALFTRGQTQALVITTLGSVRDEQSVDTLEEDTTRKFYLHYN
                     FPPFSTGEIKPRRSQSRREIGHGALAEKAVKAVVPKETEFPYTIRVVSEILESNGSSS
                     MATVCGTSLSLMDAGVPIAKPVA"
     gene            complement(1464..1730)
                     /gene="rpsO"
                     /locus_tag="PKJ95_05775"
     CDS             complement(1464..1730)
                     /gene="rpsO"
                     /locus_tag="PKJ95_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNR65780.1"
                     /translation="MVKKELKDKIIKEYQHHEIDTGSTEVQVAILTERINSLTKHLNE
                     NKKDKHSKYGLLRMVGKRRNLLNYLKDNDVEKYRKLIEQLNLRK"
BASE COUNT          643 a          409 c          299 g          843 t
ORIGIN      
        1 agccaccggt tttgctattg gaacccctgc atccattaat gagaggctgg ttccacatac
       61 ggtagccata gaagatgagc catttgattc caatatttca gaaaccacac gaatagtata
      121 aggaaattct gtttctttag gaacaactgc tttcaccgcc ttttcagcta aggcaccatg
      181 tccgatttcc ctacgggatt gagatcttct cggtttaatt tctccggtgg agaaaggagg
      241 aaaattataa tgtaaataaa atttacgggt agtatcctct tccagggtat ctacagattg
      301 ttcatccctt acagaaccca gagtagtaat gaccaaagcc tgggtttgtc ctctggtaaa
      361 aagagcgctt ccatgaactc tcggcaaaat atctatttcg cattcaatag ctctaatttc
      421 atcaggttta cgatggtcta ctcttaaccc tttttcaata atcaatcgtc tcattaagtc
      481 cttacataag gaatcatata cttcattgat tatgagaaca tcgagttctt cttcaggact
      541 aagattattt tttagttctg tgatgatttc ttcttttaag ttttctaatt tttcttctct
      601 ctcagctttt tccctggtaa taaggaattc actgatttgt tctgaatagt ccttttttat
      661 cagctcataa aaactgtttc tttcttctat taaagattct tccaattgcc agcttttgtt
      721 atttttctca ggatttattt tttgagcaaa tccaatttgc atttccacaa tttttttaat
      781 ctctgtctga gcagtttcaa ttgcttctaa aacgattcct tcagataatt ccctggcttc
      841 tccctctaac attattactg aatcttttgt tcctgcaaca actatattaa gctgacattc
      901 cgttaattct tctactgtag gatttatcaa aaaattaccg tttaaatacc ctacacgtat
      961 ggcacctatt ggtttataaa aaggaatgtt agaaatataa agagcacagg atgcaccaat
     1021 tatagatata atatcaggaa ggtttttctg gtcatacgat aaaactgatg cgattatctg
     1081 gacatcgttg taataacctt caggaaacaa gggacgtatt ggtctatcaa taagtctgga
     1141 agtaagaatg gcattttcac tcggtttccc ttctcgtttg aaaaatccac caggtatttt
     1201 accggcggca taaaatttct cttcataatc gaccagcaat gggaaaaaat cagtaccttc
     1261 cttaggttct ttggatgcta ctgcagtaac aagtaccaca gtttcaccat aggtgaccat
     1321 aactgagcca ttggcttgtt ttgccaattt acccgtttca atttttaact ttttaccacc
     1381 aacttctatt tctgtactat gaataatact tttaatcata aatcaggcat ctccttgaat
     1441 aagcatacaa tattttaaat tcattacttt cttaaattta attgttcaat taatttacga
     1501 tatttttcaa cgtcattatc ttttaaataa ttcaggagat ttcttctttt cccaaccatt
     1561 ctcaataatc catacttgga gtgcttgtct tttttattct catttaaatg cttggtgagg
     1621 ctgtttattc tctctgttaa aatagccacc tgaacttctg ttgaaccagt atctatttca
     1681 tgatgctggt attcttttat tattttatct tttaattctt tcttaaccat tttcgctaaa
     1741 ctctccttct gacctaatgg taattgttgt atatgctaac ttcttgaaat tagatatcat
     1801 ttttaataat atattttgaa acatttaata tttattttga atattccggc atgatgttta
     1861 ccagattgaa aggaaagtag gttcttttga aattttatcc aaaatgagga ttatttcctt
     1921 cttttaatcc ttatgcaatc ttacatcatc aatatatttt accataatta aaaatttcat
     1981 tcaaatatat tttaataaat cctataatag aaaatatata tcattaaata actgctcatt
     2041 acctataaaa actctatgat acccagtttg gaaaacaaat tcttatggtt atcccctttt
     2101 gttatctctc atcaattctc tgtttttttt aattatttgg cttgtaatat tttccattat
     2161 aattaaatat ttttttgttt gctgaaaaaa atat
//
LOCUS       DAOQWT010000275         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_57409_length_2180_cov_3.212235, whole genome shotgun sequence.
ACCESSION   DAOQWT010000275 DAOQWT010000000
VERSION     DAOQWT010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57409_length_2180_cov_3.212235"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1291
                     /locus_tag="PKJ95_05780"
     CDS             137..1291
                     /locus_tag="PKJ95_05780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNR65781.1"
                     /translation="MRILIVGAGPVGCYTAQLLKERGYHSVLLEEHASLGKPVHCAGI
                     ISSDLLTILQPLISDGAVKSIINEFSINTPWEGEFSICKSGVAAVLDREKFDSDLGKG
                     LDVHLGERICSIEKNNNRYKVRTEQGKEYEADLLIGADGAGSMVRKFLLQNYGKKGSI
                     ANIGINYYTGMQYRIRLHNSAPEMAQDSIKVFFNSDIHFFIWMIKEDSYIIRIGVIHN
                     QNKDILDNFTRKEGIKGEIIDIIPGRIPLGLLPTYNNQIALVGDAACQVKPLTGGGLS
                     FGLQSARILADCIREGKLEQYDSRWKKKFGPEIRFGMKARKIYENLNYEQRREVFKIF
                     RKNSDFIEMMVDYDHHSKLFREAFRKPQILLDAGKLFSFYLEDLVSEFIS"
     gene            1324..>2180
                     /locus_tag="PKJ95_05785"
     CDS             1324..>2180
                     /locus_tag="PKJ95_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNR65782.1"
                     /translation="MDYQKKYRQWLDSSYLKEEDKEELRSIAGNEEEILDRFYKDLDF
                     GTGGIRGKIAIGANRMNIYTVGQTTQGLANYLLQKVEKTQEKGVVIAHDPRKMSREFT
                     EQAAQVLTANGIKTYLFDDIRPTPELSFAVKMIGAAAGIVITASHNPPDYNGYKVYAE
                     NGAQILPEIADEIIAEISKIENYSKVKLISLEEAKERKLCKILGEVFDRVYYEKVKTL
                     SLSKDEEIDEEIKVVYTPLHGTGNIPVRTILKDRGFKNIFVDEEQAKPDARFSTVTSP
                     NPENPEAFD"
BASE COUNT          759 a          321 c          478 g          622 t
ORIGIN      
        1 acatataaat accttctcag aaaaaacact gcaaaatgta aacttctttc ctttaaaaat
       61 actattataa aatgtataat aaaagataat caataagtaa ataaatgata aaaactgtta
      121 ataggttatt tttataatga ggatattgat tgttggcgca ggtccggtag gatgttatac
      181 tgcacagtta ttaaaggaaa gaggttatca ttcggttctt ctggaagaac acgcctcact
      241 tgggaaacca gtacattgtg ctggaataat ctcctcagat ttactaacta ttttgcaacc
      301 tcttatttct gatggtgcag taaaaagtat aattaatgaa ttttccatta acaccccatg
      361 ggaaggggag ttttcaatct gcaaaagcgg agtggcagct gtattggata gagaaaaatt
      421 cgattcagat ttaggtaaag gcctggatgt acatctgggt gaacgcattt gctcaattga
      481 gaaaaataat aatcgttata aagtaaggac tgaacagggg aaggaatatg aagcagacct
      541 attaattggc gcagatggtg ccggttctat ggttcgcaaa ttccttttac agaattatgg
      601 caaaaaaggc agtattgcca atatcgggat aaactattat acaggtatgc aatacaggat
      661 aagattgcat aactcagctc cagagatggc acaagattca attaaggttt tctttaatag
      721 tgatatccac ttttttatct ggatgataaa agaagatagc tacattataa gaattggagt
      781 aattcataat cagaataaag atatattaga taattttaca agaaaggaag gaattaaagg
      841 ggagattata gatatcattc ccggtagaat ccccctcggg cttttaccca cttataataa
      901 tcagattgcg ctagttggtg atgccgcctg ccaggtaaaa cctttgactg gaggaggcct
      961 ctcttttggt ttacagtctg ctcgtattct ggctgattgt attagggaag gaaaattgga
     1021 gcagtatgac agtagatgga agaaaaagtt tggtccggag attagatttg gcatgaaagc
     1081 ccgtaaaatt tacgaaaatc tgaactatga gcagaggaga gaggtattta aaatattcag
     1141 gaagaattct gattttattg aaatgatggt tgattatgac catcattcca aattattcag
     1201 agaagctttt cgaaaacctc aaattttact ggatgctgga aagttattct ctttttattt
     1261 agaggatctg gtgagcgaat ttatttcata aaatgataac taaagtatca ggaggggctt
     1321 attttggatt atcagaaaaa atatagacaa tggcttgata gctcatatct aaaagaggaa
     1381 gacaaggaag aactgcgttc catagctgga aatgaagagg agattttaga ccgtttttac
     1441 aaagaccttg attttggtac tggaggaata aggggtaaga ttgctatcgg tgccaaccgt
     1501 atgaatatct acacagtggg acagaccact caaggactgg ctaattatct gctgcaaaaa
     1561 gtagaaaaaa cgcaggaaaa aggtgtagtg attgcccatg accccaggaa gatgtcgcgt
     1621 gagtttactg agcaggcagc acaggtttta acagccaatg gaataaaaac ttatcttttt
     1681 gatgatatac gacccacacc tgaattatct tttgcggtta agatgattgg ggcagcggcc
     1741 ggcattgtta ttaccgccag tcataacccc cctgactata atggctataa agtatatgct
     1801 gaaaatggtg cacagatatt gccggagatt gctgatgaga ttatagcaga gataagtaaa
     1861 atagaaaatt attcaaaagt taagttgatt tctctggaag aagccaaaga aagaaaatta
     1921 tgcaaaatac tgggggaagt atttgaccgg gtttattacg agaaggttaa aacattgtca
     1981 ttgagtaagg atgaagagat agatgaagag ataaaagttg tatatacccc cttacatgga
     2041 accgggaata ttccagtaag aacgatttta aaggataggg gttttaaaaa tatatttgtt
     2101 gatgaagagc aggcaaaacc tgatgcacgg ttttctactg taacatcacc caatcccgaa
     2161 aatccagaag catttgatag
//
LOCUS       DAOQWT010000276         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_57486_length_2178_cov_3.875177, whole genome shotgun sequence.
ACCESSION   DAOQWT010000276 DAOQWT010000000
VERSION     DAOQWT010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57486_length_2178_cov_3.875177"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /gene="dapF"
                     /locus_tag="PKJ95_05790"
     CDS             <1..393
                     /gene="dapF"
                     /locus_tag="PKJ95_05790"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNR65783.1"
                     /translation="QYFRITCVSMGNPHCVIFTEDVDSCKVSEVGPIIENHHLFPEKT
                     NVEFVQPLNKHEIKLRVWERGVGETMACGTGASAAVVAGVLNKRINNKTEVHLKGGNL
                     EIEWVGETEGHVFMTGPAISVFSGIVSI"
     gene            548..>871
                     /locus_tag="PKJ95_05795"
     CDS             548..>871
                     /locus_tag="PKJ95_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNR65784.1"
                     /translation="MRIVVKFGGTSIQDTERMEKAAHSVIKKIKDGNEMLIVVSAMGK
                     TTDNLISLLEETTKSSYDKSEYNEFISFGERMSARLMFSALKSRGIKSVVFDPVKEDF
                     PLITDS"
     assembly_gap    872..881
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <882..1779
                     /locus_tag="PKJ95_05800"
     CDS             <882..1779
                     /locus_tag="PKJ95_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNR65785.1"
                     /translation="SNLIEANILLEESREQCQKYIEPLLKEGVIPIVCGFLGRSNKGG
                     SITTLGRGGSDISAFALGKIINADEVIIVTDTAGVASADPRLVKDTRILPKISVEEMG
                     IMADGGAKVLHPRALHFKEENMKARIINFQKGDLDSEGTEIEGRFLSKMEIFPEKLCL
                     LTVVGNQILETPGLLKEIITPLAEKNISIQGVGTGRNYIGLYLLEQNARMAYDLIHPI
                     ILKNHSLKSVSLKKDIALLILNSRKFIDTPGIIERITRPLAENKINIIEISTMKTDIL
                     LFVDWHNKDTIYKLINNSLENE"
     gene            1847..>2178
                     /locus_tag="PKJ95_05805"
     CDS             1847..>2178
                     /locus_tag="PKJ95_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNR65786.1"
                     /translation="MFYGSIVAMITPFDEYNRIDEEGIKRLVDFHINNGTNGIVPCGT
                     TGESPTLTHEEHKKVIEITIKAAAGRIPVIAGTGSNSTEEALELTASARDMGADGVLL
                     VIPYYNKPT"
BASE COUNT          781 a          311 c          459 g          617 t
ORIGIN      
        1 caatacttta gaataacctg tgtctctatg ggaaatcccc actgtgtcat ctttactgaa
       61 gatgttgaca gctgcaaggt atctgaagtt ggtccaatta ttgaaaacca ccatcttttc
      121 ccagaaaaaa ccaatgtaga atttgttcag cctctcaata aacatgaaat taaactcagg
      181 gtttgggaaa ggggtgtcgg agaaacaatg gcttgtggaa caggagccag tgcagctgtg
      241 gtcgccggag tgctcaacaa aagaattaat aataaaacag aagtccactt aaagggtgga
      301 aatttagaaa ttgaatgggt tggagaaaca gaaggtcatg tttttatgac aggacctgca
      361 ataagtgtat tttccggtat agtatcaatc taaagactga agactgtagt tttaccaaga
      421 ccctttttat atctaaagac aaactgatta tcttttgaga tagcaaaggt atgggagtta
      481 gtatctataa aatagatata ttaaaataag cagtaatata aatgataaga aaagataggt
      541 gattgacatg agaattgttg ttaaatttgg aggaacaagc attcaagata ctgaaagaat
      601 ggaaaaagcc gcacattcag ttataaaaaa gataaaagat gggaatgaga tgttaatagt
      661 ggtttctgcc atgggaaaaa ctactgacaa ccttatatct ttattagaag aaaccactaa
      721 atcgagttat gacaaaagcg aatataatga gttcatctct tttggtgagc gaatgagtgc
      781 cagattgatg ttttccgcct taaagtcgag aggtattaaa tcagtcgtgt tcgatcccgt
      841 taaagaagat ttcccactca tcaccgattc gnnnnnnnnn ngagtaattt aattgaggca
      901 aatattcttt tagaagaaag tagagaacag tgtcagaaat acattgaacc gttgttaaaa
      961 gaaggagtta ttccaatcgt atgcggtttt ttaggcagaa gcaataaagg gggtagtatt
     1021 actactcttg gaaggggcgg cagtgatatt agtgcttttg ctctagggaa aataattaat
     1081 gcagatgaag taataatagt aactgatact gccggtgttg cctctgctga ccctcgtttg
     1141 gtaaaggata ccagaattct gcctaaaata agtgtagaag aaatgggaat tatggcagat
     1201 ggtggagcaa aggttcttca tccaagagca ttacatttta aagaggaaaa tatgaaagcc
     1261 aggataatca attttcaaaa aggagatctt gattcagaag gtacagaaat agagggtagg
     1321 tttttaagta agatggagat atttccggaa aagctttgtc tgctaacagt tgttggaaac
     1381 cagatcttag aaactcctgg tttattaaaa gagattatta ctccattagc tgaaaagaat
     1441 attagcatac agggtgttgg taccggtagg aactacatag gtttatatct gctggaacaa
     1501 aatgccagga tggcatacga cctgattcac cctattattc tcaaaaacca ttctttgaaa
     1561 tctgtttctt tgaaaaagga tattgccctt ttgatattaa atagtagaaa gtttatcgat
     1621 acacccggta ttatcgaaag aataaccaga cccttggcag aaaacaaaat aaacattata
     1681 gagatatcaa caatgaaaac tgatatattg ttatttgttg attggcataa taaagatacc
     1741 atatataaat taataaataa ttcattagaa aatgaataac aaaaaaatga taaatacaaa
     1801 tagttctctg attaaattaa ttaaaataca gaaataggag ttagcgatgt tttacggttc
     1861 aatcgttgca atgatcaccc cctttgatga atataataga attgatgagg aaggtattaa
     1921 aaggttagtc gattttcata ttaataatgg aaccaatggt atagtgccat gtggaacaac
     1981 aggtgagtcg cctacactga cacatgaaga gcataagaaa gtaattgaga ttaccattaa
     2041 agctgctgca ggaagaattc cggtaattgc gggaactggc tctaattcta ctgaagaagc
     2101 gctggaactt acagcaagtg ccagagatat gggagcagac ggagtgcttt tggttattcc
     2161 ttattataat aagcctac
//
LOCUS       DAOQWT010000277         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_57835_length_2170_cov_2.343262, whole genome shotgun sequence.
ACCESSION   DAOQWT010000277 DAOQWT010000000
VERSION     DAOQWT010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57835_length_2170_cov_2.343262"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..>1053
                     /locus_tag="PKJ95_05810"
     CDS             85..>1053
                     /locus_tag="PKJ95_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014733980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HNR65787.1"
                     /translation="MGAAGRDFHNFNVYFRNNENFKVVAFTATQIPDIAGRKYPSVLS
                     GQLYPDGIPIFPEEELPGLIERFQVNQVILAYSDLPHQYVMDRASLVLAMGADFRLMG
                     PHNTMLKAKVPLISICATRTGSGKSQTTRKVASILKEMGKKVVVIRHPMPYGDLSEQI
                     WQRFETYKDLDKYKCTIEEREEYEPHIDLGNIVYAGVDYGEILKRAEQEAEIILWDGG
                     NNDIPFYYTDLHIVVADPHRAGHEMTYYPGQVNIRMADVVIINKIDTASPENVALLRN
                     NIFKMAPEAILIEAASPIKVEFAEKLRGKRVLVVEDGPTLTHGGMEF"
     assembly_gap    1054..1063
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1064..1388
                     /locus_tag="PKJ95_05815"
     CDS             <1064..1388
                     /locus_tag="PKJ95_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HNR65788.1"
                     /translation="GAGMVAAQKYGVKEVIDPRPFAIGSIKNTYEIYPLIGKVLPAMG
                     YGEEQVKELESTINRSDAEMVIIGTPIDLSRIMHIGKDNVRVTYDLQEIGKPDLKEIL
                     LKRFS"
     gene            1447..2170
                     /locus_tag="PKJ95_05820"
                     /pseudo
     CDS             1447..2170
                     /locus_tag="PKJ95_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
BASE COUNT          738 a          300 c          494 g          628 t
ORIGIN      
        1 aattgattta ttattcttta aataataaaa ttagaaataa atatctaaga ggtgattttt
       61 ttgataaaac aaaaagttat tattatgggt gcggctggta gagattttca taatttcaat
      121 gtctattttc gaaataatga gaatttcaag gtagttgcct ttaccgcaac acagattcct
      181 gatattgctg gtagaaaata tccgagcgta ttaagtggcc agctatatcc tgatggaatc
      241 ccaatctttc cagaagaaga gttgcctgga ctaatagaga gatttcaagt taatcaggtt
      301 attttagcgt atagtgattt acctcatcaa tacgtaatgg acagagcgtc actggttttg
      361 gctatggggg cagacttcag gttaatgggt cctcataata caatgttaaa agctaaagtg
      421 cccttgattt cgatttgtgc aacccgtacc ggcagcggta agagtcagac tactcgtaaa
      481 gtagctagca tattaaaaga aatggggaaa aaagttgtag taataagaca tccaatgcct
      541 tatggggatt taagtgaaca aatctggcag agatttgaga cttacaaaga tttagataaa
      601 tataaatgca ccatagaaga aagggaagaa tatgaacccc atattgattt aggaaatata
      661 gtctatgccg gggttgatta tggtgagatt ttaaagagag ccgaacaaga ggctgaaata
      721 atcctatggg atggtggcaa taacgatatt cccttttatt atacagacct gcatattgta
      781 gtggccgacc ctcatcgtgc aggtcatgaa atgacctatt accccggaca ggtgaatatt
      841 agaatggctg atgtggtaat cataaataaa atagatacag cgagcccaga aaatgttgct
      901 cttttaagaa acaatatttt taaaatggca cctgaagcca tcctgattga agcagcttct
      961 cccataaaag ttgaatttgc tgaaaaatta agaggaaaaa gagtgctggt tgtggaagat
     1021 ggaccaactt taacccatgg aggtatggaa tttnnnnnnn nnntggtgct ggtatggttg
     1081 ctgcgcaaaa atatggggtc aaagaagtaa tagatcccag accatttgca ataggaagta
     1141 ttaaaaatac ttacgagata tatccgctta taggtaaagt attgccggca atgggttatg
     1201 gagaagaaca ggtgaaagaa cttgagtcta ccataaatag gagtgatgct gaaatggtca
     1261 tcattggaac tccaattgac ctgagtagaa taatgcatat tggcaaagac aatgtcaggg
     1321 ttacttatga tttacaggag attggaaaac cagatttgaa agaaattctc cttaaaagat
     1381 tttcttaaag cggtagctta aagcggtgtt ttacttgaaa tgaaatatta ttgaaaggag
     1441 gcttttatga tcttaggaca atctttaata tcatttatat atgaaccagc aaaagtaaac
     1501 agaggttata ctgatagggt tctttatctt aatttgtcta aaaaagatat aaaaattaaa
     1561 gaagttcctc caaaaattaa agatactctg attggtggta gaggctacgg tttgtggtat
     1621 ttgtggcagg caactaaatc ggatacaaaa tggaatgatc cagagaatga actggtattc
     1681 tgtactggac ctctttgagg ggtgactcaa tattcagggt gtggaaaaag tcatgtagtg
     1741 agtatttccc cggaaactgg aagtgccaat gataataatg ctggtggtta ttttgctcca
     1801 tatttaaaat tctcaggttg ggatgctttg gaagtacagg gaaaatctga gcaggattgt
     1861 ataattttta ttgatggaaa taatggagag gtaactgtta acacctatac tggcaaagag
     1921 aagaactctt accgtctagt agaggagttg acagagtatt ttgctgacaa tgaaaaagat
     1981 aaatccaata ttagtatagt atctgcagga gagggagcca aaaattcctt gttgggcatt
     2041 ttgaatttta gctggtatga ccggaaaaga ggtaaagtca ggtataaaca ggctgcacgt
     2101 ggtggtccgg gaacagtatt tcgagacaag aaaatattgg ctatcatagt aaaatatagt
     2161 ggcattggtg
//
LOCUS       DAOQWT010000278         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_57867_length_2169_cov_3.105487, whole genome shotgun sequence.
ACCESSION   DAOQWT010000278 DAOQWT010000000
VERSION     DAOQWT010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57867_length_2169_cov_3.105487"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKJ95_05825"
     CDS             complement(<1..211)
                     /locus_tag="PKJ95_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNR65789.1"
                     /translation="MAKAIGIDLGTTNSVVAIIEGGKPVIIENKEGSRTTPSMVAFTK
                     KGERLVGLVAKRQAVTNSKNTVYSVK"
     gene            complement(305..931)
                     /gene="grpE"
                     /locus_tag="PKJ95_05830"
     CDS             complement(305..931)
                     /gene="grpE"
                     /locus_tag="PKJ95_05830"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNR65790.1"
                     /translation="MVQNEQQKMEEEILPDVQEEKYNRMKKTELMATIASRDVQLQEM
                     EEKWEKREAELEKIRENEKSYKEQLIRMQADFENYKKREEKKKQEFMEYAKQELICQL
                     LTVIDNLERASSYTKNQSEEGNNENIKEGLRGILKIFRDILEKEGLKPIEAVGEKFDP
                     YCHEAIMNVESEKYPEDTIVEELVKGYYLKSKVIRPSVVKVSKGTSDK"
     gene            complement(987..2015)
                     /gene="hrcA"
                     /locus_tag="PKJ95_05835"
     CDS             complement(987..2015)
                     /gene="hrcA"
                     /locus_tag="PKJ95_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNR65791.1"
                     /translation="MIINNRMSTILNAVVHLYITNAEPVSSEMIVSYYGLAISTATVR
                     NDLQLLEKYGFLKKPHTSAGRIPTDKGYRFYVDQLMEKEIVHLTEEEKQDIKEHYKIA
                     RDYNEIMKVTSVLISRLTQNLGVVLAPNMIKDTLKDIHFVTLNNQRILATLVTNSGLF
                     FQKMMRVNCDISEENLEAISQIIKKEFYGKSLEVVNDEMLNQIYHYYFKYQTLDSIWN
                     LIENCFDFTEEETKIFFGNRSYILKQPEFKEIERLNYLFDFIEEEDNLIKIIKKSIFN
                     KQIDIIIGSELGQEIMENCSVITRQYLLKGKLRGAISVLGPTRMNYSKSVSVLDFISH
                     QLSEILSE"
BASE COUNT          598 a          433 c          274 g          864 t
ORIGIN      
        1 gtttaacaga ataaacggta ttttttgaat tggttactgc ctgtcttttg gcaaccagtc
       61 caactaatct ttctcctttt ttggtaaaag ccaccataga aggcgtagtt ctgctgcctt
      121 ctttgttttc tataatgaca ggtttgccac cttctataat ggcaacaact gaattagtag
      181 ttcccaaatc aattccaatt gcttttgcca taattatttt ctccttttat ttattattag
      241 tattgtcacc tttttcatta ttattattat tctttaaatc tatacctttt tctccctttt
      301 ctttttattt atctgaagtt cccttggaaa ccttaaccac agatggtctg attacttttg
      361 attttaaata gtaacccttt accaattctt caacgatggt gtcctccgga tatttttcag
      421 attcaacatt cataatggct tcatggcaat aggggtcaaa tttttcacct acagcctcta
      481 taggtttgag tccttctttt tccagtatat ctctgaaaat ctttaaaatc cctcttaacc
      541 cttccttaat attctcatta ttcccttcct ctgactgatt tttagtatag gaagaagccc
      601 tttctaaatt atcaattact gttaataact gacagattaa ttcctgtttg gcatattcca
      661 taaattcttg cttttttttc tcttctcttt ttttataatt ttcaaaatca gcttgcatcc
      721 tgattaactg ttctttataa gatttttcat tttctcttat tttttcaagt tctgcctctc
      781 ttttttccca tttttcttcc atctcctgca gttgcacatc cctggaggca atggttgcca
      841 taagttctgt ttttttcatt ctattatatt tttcttcctg aacatccggc aaaatttctt
      901 cttccatttt ttgctgttca ttttgaacca ttatttatct cccctgttta acatatttga
      961 aacattctta ctcagttgtc catagcttac tctgacaata tttcacttaa ttgatggcta
     1021 atgaagtcca gcacggaaac agatttagaa taattcatcc ttgttggtcc taaaacactt
     1081 atagcacctc ttaattttcc tttcaagagg tattgtctgg taatgacact acaattctcc
     1141 ataatttcct gccccagttc gctaccgata atgatgtcta tctgtttatt gaaaatggac
     1201 ttttttataa ttttaatcag attatcttct tcttcaataa aatcaaagag gtagttcagt
     1261 ctttcaattt ctttgaattc cggttgtttc aaaatatagc ttcggttgcc aaagaatatc
     1321 ttggtttctt cttctgtaaa atcaaaacag ttttcaatca aattccagat ggagtccaaa
     1381 gtctggtatt tgaaatagta gtgataaatt tgattcaaca tctcatcatt aactacttcc
     1441 aaactcttgc cataaaattc tttttttata atctgagaaa ttgcctctaa attttcttca
     1501 gaaatatcac aattaaccct catcattttt tgaaagaaca atccgctatt ggttaccaga
     1561 gtagctaaaa tacgttgatt atttaaagtg acaaaatgaa tatctttcaa agtatcttta
     1621 atcatattag gagctaaaac cacccccaga ttttgagtta atctggaaat taatacagag
     1681 gtaaccttca ttatctcatt gtaatctctg gcaatcttgt aatgctcttt tatatcctgt
     1741 ttttcctctt cagtcagatg gactatctcc ttttccatca gttgatctac atagaatcga
     1801 tagcctttat ccgtcggtat tcgtcctgct gaagtatggg gtttttttaa aaacccgtat
     1861 ttctccagca attgcaaatc attacgaaca gtcgctgtgc taatagctaa accataataa
     1921 gacactatca tctccgagct aaccggttca gcattagtaa tataaagatg aactacggca
     1981 tttaagatag tgctcattct attattaatt atcattgttc ttacctcgaa ttagcactct
     2041 atggttgaga gtgctaaatt cttttttaga ataccactca aagattcttt tgtcaatact
     2101 tctgccagaa tatctgtgat taatttatga taatgtcacc ttattaatta tcttgataaa
     2161 atgtcacta
//
LOCUS       DAOQWT010000279         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_57951_length_2167_cov_3.037879, whole genome shotgun sequence.
ACCESSION   DAOQWT010000279 DAOQWT010000000
VERSION     DAOQWT010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57951_length_2167_cov_3.037879"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKJ95_05840"
     CDS             <1..187
                     /locus_tag="PKJ95_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNR65792.1"
                     /translation="ASHARIQLTTISIAKKKMANLAAELLVKKIMGKKEESKKIQIKL
                     TPKLILRKTTDKVNRIK"
     gene            331..819
                     /locus_tag="PKJ95_05845"
     CDS             331..819
                     /locus_tag="PKJ95_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNR65793.1"
                     /translation="MKIGIMSDSHDNLVKIRRAVEIFNREKISILFHAGDFVSPFTAR
                     EIKNIYCPFLGVFGNNDGDRLFLLEQFQKIGSIYPEPYKTEIEDKKIIMFHKNDVIAD
                     LARSKQYDLIIYGHTHKIDYYKEGKTTVINPGECGGWLTGKSTVAIFDLADLNVHMIE
                     LT"
     gene            complement(890..>2167)
                     /locus_tag="PKJ95_05850"
     CDS             complement(890..>2167)
                     /locus_tag="PKJ95_05850"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HNR65794.1"
                     /translation="NENVYTHYQGTGGIPISSFLRRLLFTIQFRDVNILLTGSFTRES
                     RIMLYRNVVDRAIKIAPFLVYDRDPYIVISDDGRLYWIQDAYTISNHFPYSRPYNKMF
                     NYIRNSVKIIVDAYNGNINFYIVEPEDPIINVYQKIFPGLFKNIKDMPQDLRRHIRYP
                     VDLFKVQSEMYSTYHMQNPDVFYNKEDYWNIPNEIYATEEIILEPYYLIAKLPGFEKE
                     EFILITPFTPTNKNNMIAWLAARNDGEEYGKMLVYKFPKDKLIYGPMQLEALIDQDSE
                     ISQQITLWSQSGSTVIRGNLLAIPIEDTVLYAEPLYLRAEKGEIPQLRRVIVSDGVNL
                     TMDTNLDRALRRLFDRPVDEKREAILKEGVTLNDLINNAIRYYREAKDASREGNWALY
                     GQRLEQLEETLEILKGFVEQDELQELQDLLEEP"
BASE COUNT          732 a          365 c          369 g          701 t
ORIGIN      
        1 ggcttcccat gctcgtattc aactcaccac tatatctata gcaaagaaaa aaatggcaaa
       61 cttagcggca gaattgttag ttaaaaaaat aatggggaaa aaagaagaat caaaaaaaat
      121 tcaaattaaa ttaacgccaa aattgattct tagaaaaaca actgataaag ttaataggat
      181 aaaatagttc cttgctttat caataataaa ttttatcaaa ttaaatggtt tttatagaga
      241 ttgggggagt cattataaca tttgggtaaa aaagtttgtc aaaatagata tttataatta
      301 ctgataaaac attttgcaaa aggggtataa gtgaaaattg ggattatgtc tgatagtcat
      361 gataatttag ttaaaataag gagggcagta gaaatcttta atagagaaaa gatttctatc
      421 ttgtttcatg caggtgattt tgtctcacct tttactgcca gagaaataaa gaatatttat
      481 tgtccttttc taggagtgtt tggtaacaat gatggtgacc gactatttct gttggaacaa
      541 ttccaaaaaa ttggctcaat ctatcctgag ccctataaga cagaaattga agataaaaaa
      601 attattatgt ttcataaaaa tgatgtaata gccgatttag ccaggagcaa acaatatgac
      661 ctcattattt atggacatac acacaagatt gattattata aagagggtaa gacaacggtt
      721 atcaatccgg gagaatgtgg cgggtggctc accgggaaaa gtactgtcgc aatttttgat
      781 ttggctgact taaatgtaca tatgatagaa ttaacctaaa aatattaagc aactccaaac
      841 aatttacctt tattgcactt aatattaata atttacaatt ttataataat tatggctcct
      901 caagtaaatc ctgtaattct tgtaattcat cctgctctac aaaacctttt aatatttcta
      961 aggtctcttc tagttgttct aaacgctgcc cgtataatgc ccagtttccc tctctggagg
     1021 catcctttgc ttccctgtag tatcgaatgg cattattaat taaatcgttt aaggtaaccc
     1081 cctcttttaa gatggcttct ctcttttcat caactggcct atcaaacagc cttcttaagg
     1141 ctctgtctaa atttgtatcc atagttaaat ttaccccatc ggatacaata acacgtctca
     1201 gttgtggtat ttcacctttt tcagctctca gatacaatgg ctctgcatag agaaccgtat
     1261 cttcaatagg aatggccagt aaatttcccc tgatcaccgt tgaaccactc tggctccaaa
     1321 gtgttatctg ctgggagatt tcagaatcct ggtcaattaa tgcttccaat tgcataggac
     1381 cataaatcaa tttatctttg gggaatttat aaaccagcat cttcccatat tcttccccat
     1441 cattacgagc cgccagccag gcgatcatat tattcttatt ggtaggagta aaaggtgtta
     1501 tcaggataaa ttcttctttt tcaaaaccgg gtaatttggc aatgaggtaa taaggttcca
     1561 gaataatttc ttctgtagca tatatttcat tgggaatatt ccagtaatct tctttattat
     1621 agaatacatc cggattctgc atgtggtaag tagaatacat ttctgactgt actttaaaaa
     1681 gatcgactgg ataacgaata tgccggcgaa gatcctgggg catatcttta atatttttaa
     1741 ataaaccagg gaaaatcttc tgatagacat ttataattgg gtcttctggt tccacaatat
     1801 aaaaattgat attaccatta taggcatcaa caatgatttt tactgaatta cgtatataat
     1861 tgaacatctt attatagggt cgggaatatg gaaaatgatt ggaaatagtg taggcatcct
     1921 gtatccagta cagtctgcca tcatctgaaa tgacgatata agggtcacgg tcatatacta
     1981 aaaaaggtgc aattttaata gcacggtcta caacatttct atagagcatt atacgactct
     2041 ccctagtaaa actaccggtc aatagtatat ttacatctct aaattgtata gtaaaaagca
     2101 atcggcgtaa aaaagaagaa ataggtatgc cacctgtgcc ctgataatgg gtatagacat
     2161 tttcatt
//
LOCUS       DAOQWT010000280         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_58033_length_2165_cov_2.950711, whole genome shotgun sequence.
ACCESSION   DAOQWT010000280 DAOQWT010000000
VERSION     DAOQWT010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58033_length_2165_cov_2.950711"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKJ95_05855"
     CDS             <1..229
                     /locus_tag="PKJ95_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65795.1"
                     /translation="SGKSPLNGSFSGALSRGEGPERTVTHKRNSDIKPFFLMWSSHPG
                     QGVKLLHHSPGICNMDIMEDFSFLLWNNWMT"
     gene            421..1497
                     /locus_tag="PKJ95_05860"
     CDS             421..1497
                     /locus_tag="PKJ95_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HNR65796.1"
                     /translation="MGFTEDIKVLAERSLKLKEVLQTEEAVKHSLLMPFLNLLGYNVW
                     DPHEIVPEYIADLGTKKGEKVDYVILNNGEPHILIEVKGPDSKVEEHKNQLYRYFNSL
                     SKTKFAILTNGLVYQFFSDLDEPNIMDMNPFLTVDFENLKEQSIIELEKFHKKNYNIE
                     LILSTANELKYTNQIKEYLARQFKDPDEDFMKTILRNVYNGKLMSSTMLRFEPIIKKS
                     VYIFITELINEKIKAALIESEKTEKAIQPQIQELTKEVEKEDKKNITTEEELEAFVTI
                     KLLLKDLVTGDNKITYKDTASYFIIMVNDNNKNWLCRLRLGSKKKTMEFKDGEYIELE
                     TIQDLEKYKDKFVSMVSMLIPTNG"
     gene            1635..>2165
                     /locus_tag="PKJ95_05865"
     CDS             1635..>2165
                     /locus_tag="PKJ95_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65797.1"
                     /translation="MEGNEYISIQEAVSLSKKHITTIYSAIKKGKIHTIESLLRGRKI
                     KKLLKADIETLFLIDSIGHYSDHIGHYSDSIANNSNSIGLNSDTIETLKTAIEEVLEA
                     KQSQLMKPLEDQALYRCGVLENEVKHLQAEKEVLRQENELLREQVKALPDLQTIQEKE
                     KDLIIQIELERKEKEDL"
BASE COUNT          834 a          304 c          418 g          609 t
ORIGIN      
        1 aagcggtaaa agcccgttaa atggcagttt ttcaggggca ctatccaggg gagaaggtcc
       61 ggaacggacc gttacccata aaaggaactc tgatataaag ccattctttt taatgtggag
      121 ctctcaccca gggcagggag taaaactttt gcaccattca cccgggatct gtaacatgga
      181 tatcatggag gatttttctt ttctcctgtg gaataactgg atgacctgat aaaagggatt
      241 gtgactttct atgtaaccta taaactcata acaacaatct ttgttggaat tattgacctg
      301 attaataagg gattttatta acaatccgta agaatttcct aatacaaaaa ggatattctt
      361 ttttatttat ggaatatgtt attactttaa aaagtaaaat aaatgaaagg tggttgttaa
      421 atgggattta ctgaagatat taaggtacta gctgaaaggt cattaaaatt aaaggaagta
      481 ttacagacag aagaagcagt taaacactca cttctcatgc cttttttaaa tttacttggt
      541 tacaatgtat gggatcctca tgaaatagtt cctgaatata ttgcagacct tggaactaaa
      601 aaaggggaaa aggttgacta tgttattctt aataatgggg aacctcatat tctcatagag
      661 gttaaagggc cagattcgaa agtagaagaa cataaaaacc agctttacag atattttaat
      721 tcccttagca aaacaaaatt tgctatacta acgaatggac tagtatacca gttcttctcg
      781 gatttagacg aaccgaatat aatggatatg aatccctttt taacggtgga ttttgagaat
      841 ttaaaagaac agtctattat agaactggag aaatttcata aaaaaaatta caatatagag
      901 cttattcttt ccaccgctaa cgaattgaaa tacactaacc agattaagga atatttagcg
      961 aggcaattca aggatcctga tgaagatttt atgaagacca ttctaaggaa tgtctataat
     1021 gggaaattaa tgtcttctac tatgttaagg ttcgaaccta ttattaagaa gtctgtttat
     1081 atatttatta ctgaattgat aaatgaaaag ataaaggcag ctctcataga atcggagaag
     1141 acggaaaagg ccattcaacc ccagatccag gaattaacaa aagaagtaga aaaagaggat
     1201 aaaaaaaata tcaccacaga agaagaactg gaagcatttg taacaattaa acttttgctt
     1261 aaagatttag taaccggtga taataaaatt acttataaag atactgccag ttactttatt
     1321 ataatggtaa atgataataa taaaaattgg ttatgcaggt taagacttgg aagtaaaaag
     1381 aaaacaatgg aatttaaaga cggtgaatat atagaactgg agacaataca ggatttagaa
     1441 aaatataagg ataaatttgt ttcaatggta tcaatgttaa tacctactaa tggataatta
     1501 aaggccgagt aaataaggga ctataagaaa gaattattct ttttctcgtg gaatagatac
     1561 atggaacagg tgaagagact cttgttatat agagttattc acctgtttta ttatttccgg
     1621 gaaggatggc aattatggaa ggtaatgaat atatatcaat tcaagaggca gtaagtttat
     1681 cgaaaaaaca tataactact atttatagcg caataaagaa aggcaaaatt cacactatag
     1741 aatcactatt gcgaggcaga aaaataaaga aactactaaa ggcagatata gagacacttt
     1801 ttcttataga ctccatagga cactatagcg atcatatagg gcactatagc gattctatag
     1861 cgaacaatag taattctata ggactcaata gcgacactat agaaacacta aagacggcta
     1921 tagaagaagt attagaggcg aaacagagcc agttaatgaa gcccttagaa gatcaggcat
     1981 tatatagatg tggtgtttta gagaatgaag tcaaacatct tcaggcagaa aaggaagttc
     2041 tcagacagga aaacgaactt ttaagggaac aggttaaagc cctgccagac cttcagacta
     2101 ttcaggagaa agagaaagac ctgattattc aaattgagtt ggaacggaaa gaaaaagaag
     2161 atcta
//
LOCUS       DAOQWT010000281         2163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_58113_length_2163_cov_4.200190, whole genome shotgun sequence.
ACCESSION   DAOQWT010000281 DAOQWT010000000
VERSION     DAOQWT010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58113_length_2163_cov_4.200190"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /gene="glgB"
                     /locus_tag="PKJ95_05870"
     CDS             complement(<1..1139)
                     /gene="glgB"
                     /locus_tag="PKJ95_05870"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HNR65798.1"
                     /translation="MIKKNDISYQHSLLNKDDIFLFNEGTNYQLYRHLGAHLVYTGDL
                     KGVHFAVWAPNAEQVSVIGDFNGWQKTSHPLKQIHTGSGIWEGFIANLDSAMKYKYHI
                     ESRLKHYGVDKRDPFAFYGEKPPLTASMVWDLSYQWQDQDWMAQRKERNRLNQPFSIY
                     EVHLGSWCRKDSSENDFLSYREIAPLLSRYCQENGFTHIELLPVMEHPFYGSWGYQTV
                     GYFSPTSRYGTPQDLMYLIDHLHQQGIGIFLDWVPSHFPGDEHGLVYFDGTHLYEHAD
                     PRQGFHPDWKSYIFNYSRNEVRTFLISSALFWFDYYHVDGLRVDAVASMLYLDYSRKE
                     GEWIPNKYGGRENLEAIDFIKKLNETAYANFPDIQMIAEESTAWP"
     gene            complement(1123..>2163)
                     /locus_tag="PKJ95_05875"
     CDS             complement(1123..>2163)
                     /locus_tag="PKJ95_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HNR65799.1"
                     /translation="KSITEKTDFKNIPVFAGALDYQKEDGSLITMGILSSYIPNEENA
                     WDYAQDHLINYYEKVLSQICLKEKLPTLPESIFEKTVREIPELLEDLISAPFIEMIHL
                     LGKRTAEFHNALGEIVEDPDFKPEPFSLLYQRSIFQSVHSLLKRVFLSLNKNYNRYEE
                     TIQEDLKRVLSLEPEIIKHCQKIVSHKYNAKKIRIHGDYHLGQVLYTGKDFYLFDFEG
                     EPAKPLGERRLKRSPLKDVAGMLRSFQYAAFAPLIQKKVDGDCHELEHWARIWTFYTS
                     KVFLNAYLKYIVPSFLPEDESEIKQLLTIYLLEKAIYEIGYEMDHRPDWLSIALKGLL
                     FEVGAIDNDKKE"
BASE COUNT          627 a          455 c          429 g          652 t
ORIGIN      
        1 attggccagg cagtggattc ctcggcaatc atctgaatat ccggaaaatt agcataggcg
       61 gtttcattca attttttgat aaaatcaata gcctccaggt tttccctccc tccatactta
      121 ttgggtatcc attccccctc tttgcgggaa tagtccagat agagcataga ggctaccgca
      181 tctacccgca ggccgtcaac atgataataa tcgaaccaga ataaagcgct gctgatcaaa
      241 aaagtccgta cttcattacg gctataattg aagatataac ttttccagtc aggatgaaag
      301 ccctgtctgg ggtcggcatg ctcataaaga tgggtgccat caaaataaac taacccgtgt
      361 tcatcaccgg gaaagtggga aggaacccaa tccagaaaaa ttccgattcc ctgttgatga
      421 aggtggtcaa taagatacat caggtcttgt ggtgtgccat aacggctggt aggagaaaaa
      481 taaccaactg tctgatatcc ccaggagcca taaaaaggat gctccattac cggcagcaat
      541 tcaatatgag taaaaccatt ttcctgacaa taacgggaga ggagaggtgc aatttcccga
      601 tagctcaaaa aatcattttc tgagctatct tttctacacc aggagcccag atgaacttca
      661 taaatggaaa aaggctggtt taaacggttt ctttctttcc tttgtgccat ccagtcctga
      721 tcctgccatt gataactcag atcccatacc attgatgcgg tcaaaggtgg tttttctcca
      781 tagaaggcaa aggggtctct cttatccaca ccatagtgct tcaatctgga ctcaatatgg
      841 tatttatatt tcatagcact atccagatta gcaataaaac cttcccagat gccactgccg
      901 gtatgaatct gctttaaagg gtgggaagtt ttttgccagc cattaaaatc tcctattacg
      961 gaaacctgct cggcattggg agcccagact gcaaaatgca caccttttag gtctccagta
     1021 taaacgagat gtgcacctaa atgtcggtat aattgataat tggtaccttc attaaaaaga
     1081 aaaatatcat ctttgtttag cagggaatgc tggtatgaaa tatcattctt ttttatcatt
     1141 atcaattgct cctacttcaa agagaagccc tttcagagct atagacagcc agtcaggcct
     1201 atggtccatc tcatagccaa tttcataaat tgctttttcc agtaaataga tagtgagtaa
     1261 ttgttttatc tccgactcat cttccggtag aaaggaaggc actatatatt ttagataggc
     1321 attgaggaat accttactgg tataaaaagt ccatatcctt gcccaatgtt ccagttcatg
     1381 gcagtctccg tcaaccttct tttgaatcag aggagcaaag gcagcatact gaaaggagcg
     1441 gagcatacct gccacatctt tcaaagggct gcgtttcaat cttctttctc caagcggttt
     1501 ggcaggttct ccttcaaagt caaataggta aaaatccttt ccggtataga ggacctgtcc
     1561 aaggtgatag tctccgtgta tcctaatttt tttggcattg tacttatgtg atacaatttt
     1621 ctggcagtgc ttaattattt ctggttccaa ggaaaggact ctttttaaat cttcctgtat
     1681 cgtctcttca taacggttat aatttttatt taaagagaga aatactctct tcagcaaact
     1741 atggacagat tgaaagatag agcgttgata cagcaaagaa aatggttctg gtttgaaatc
     1801 agggtcttca actatctctc ccaaagcatt gtggaattct gcggttctct tgcccaggag
     1861 gtggatcatt tcaataaaag gtgcacttat caggtcttct agcagttctg gtatctctct
     1921 cactgtcttt tcaaaaatag attccgggag agtgggaagt ttttctttca gacagatttg
     1981 agataacacc ttttcatagt agtttatcag atggtcttga gcataatccc aggcattttc
     2041 ctcattggga atataggatg acagaattcc cattgtaatc agggaaccgt cttccttctg
     2101 ataatcaaga gcgccggcaa aaaccggtat attcttaaaa tcagtttttt ctgttataga
     2161 ttt
//
LOCUS       DAOQWT010000282         2159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_58300_length_2159_cov_3.007605, whole genome shotgun sequence.
ACCESSION   DAOQWT010000282 DAOQWT010000000
VERSION     DAOQWT010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58300_length_2159_cov_3.007605"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PKJ95_05880"
     CDS             complement(<1..754)
                     /locus_tag="PKJ95_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNR65800.1"
                     /translation="MGKFKRTYEEINEKIARKEAVIMTAEEVIALKEEQGIEEATKMV
                     DVVTTGTFGPMCSSGAFINLGHSDPLIRMQKVWLNDVMAYAGLAAVDIYLGATQLSES
                     ERSDYGGGHVIEEFIRGKEIRLKAVSSGTDCYPAREVEAIISKETVNQAFMVNPRNTY
                     QNYNVAVNQSAKTIYTYMGILLPELGNASFSTSGQLSPLINDPDLRTIGIGTRIFIGG
                     TEGYVIWEGTQYSPKAMPVLEKDMVARMGASLA"
     gene            complement(870..1679)
                     /locus_tag="PKJ95_05885"
     CDS             complement(870..1679)
                     /locus_tag="PKJ95_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HNR65801.1"
                     /translation="MLIIGEKLNSTRKSVREMIENRDVESVQKLARIQVEGGARMLDI
                     NSSAAGGDREENMKWMVKTVQEAVKIPLCIDSPDAGEIEKGLEAHNWTYGKALINSIT
                     GEKERIDSLLPVIKKYKCAVVVLVMDERGIPDNAQTRIEIAHKLISVLTDSGVPLADI
                     YIDPLVMPIGTNEQNGLTVLDTIKGVKDLHPDVKTIIGLSNISYGLPDRKLLNQVFMI
                     LAMSYGLDAAIVDPTDQRLMSIIRTSLTLLGKDSYCSEYIKAYREGRICGF"
     gene            complement(1728..>2159)
                     /locus_tag="PKJ95_05890"
     CDS             complement(1728..>2159)
                     /locus_tag="PKJ95_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873382.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNR65802.1"
                     /translation="HAGMELLRPKLVETGVKNIGKVVIGTVKGDLHDIGKNLVKMMLE
                     GAGFEVIDLGADVSVEKFVSAVKEYHPDILGMSALLTTTMLNMSEVIEALQTADLRGK
                     VKVMVGGAPITKHYAEQIGADGYSPDAATAVEDARLLLTLK"
BASE COUNT          594 a          510 c          317 g          738 t
ORIGIN      
        1 gagccaggct tgctcccatt cttgccacca tgtctttctc taaaacaggc atagctttgg
       61 ggctgtattg ggtaccttcc cagattacat atccttctgt accacctata aatattcggg
      121 ttcctatacc tatagttctt aaatcagggt cattaattaa ggggctcagc tgtccactgg
      181 tgctaaaact ggcattacct aattcaggta acaaaatacc catataagta tatatggtct
      241 ttgctgattg atttacagct acattatagt tttgataagt atttctggga ttgaccataa
      301 aagcctgatt tacagtctct ttggaaataa tggcttcaac ttcacgggca gggtagcaat
      361 ccgtcccact tgaaaccgcc tttaatcgaa tttctttccc tctaatgaat tcttcaataa
      421 catgaccacc accatagtcg cttctttcag attctgacaa ttgagtagca cctaaataaa
      481 tatctacagc tgccaacccg gcataggcca taacatcatt gagccatact ttctgcatac
      541 gtataagagg atcactgtga cctaaattga taaaggctcc tgaggaacac ataggaccaa
      601 aagtaccggt ggtaaccaca tccaccattt tagttgcctc ttctattccc tgctcttctt
      661 ttaaggcaat tacttcttca gcagtcataa ttactgcttc tttccgagct atcttctcat
      721 taatttcttc atatgttctt ttaaacttgc ccatgaaatt tcctcattcc tttctcatta
      781 aatcttatta ttacagtgtt actacttttc ataacaatat tcccatagat attgtttttc
      841 caaaatcaat ggtcaaaaca ttataattgt taaaaaccac atattctgcc ttcccggtaa
      901 gccttaatat actcactaca ataactatct ttacccagca aggttaggga agttctaatt
      961 atgctcatca gcctttggtc ggtggggtca actatagcgg catccaggcc atacgacata
     1021 gcgagaatca taaaaacctg atttaacagt tttctgtccg gcaaaccata agaaatattg
     1081 ctcagaccta ttatggtttt aacatcagga tgtaaatctt ttacaccttt tatagtatct
     1141 aatactgtca atccattctg ttcatttgtt ccgatcggca ttaccagagg gtcaatatat
     1201 atatctgcca gaggtacacc ggaatctgtt aatacactta tcaatttatg agcaatctca
     1261 atacgagttt gggcattatc agggataccc cgctcatcca taaccagtac cactacagca
     1321 catttatatt tttttataac aggaagtaga ctgtctattc tttccttttc accggtaata
     1381 gaattaatca atgcttttcc ataagtccaa ttatgggctt ccaaaccttt ttctatctct
     1441 cctgcatcag ggctatcaat acataggggg atttttactg cttcctgtac agttttaacc
     1501 atccatttca tgttttcttc acggtcacca ccagcagcag atgaatttat atctaacatc
     1561 cttgctccgc cttctacctg tatccgggct aatttctgta ctgattccac gtctctattt
     1621 tcaatcatct ccctaacact ttttctggta gagtttaatt tttctccaat aatcagcata
     1681 actctgaatc tcctttttaa taaaatgcta tggtgttgtg gttgatttta ttttaaagtc
     1741 aacaacaatc tggcatcttc tactgcagtg gctgcatcag gagagtagcc atcagcaccg
     1801 atttgttcgg cataatgctt tgttataggc gcccctccta ccattacctt tacctttccc
     1861 cgtaaatctg ccgtttgaag ggcttcaatt acttctgaca tatttaacat agttgtagtt
     1921 aaaagagctg acattcctaa tatatctgga tgatattctt ttactgcaga tacaaacttc
     1981 tctactgaga catccgctcc taaatctatt acttcaaacc ctgccccctc taacatcatt
     2041 ttcactaaat ttttccctat atcatgtaaa tcccctttga ccgtgcctat cacaactttg
     2101 ccgatatttt tgactcctgt ttcaactaat tttggtctta acagttccat tccagcatg
//
LOCUS       DAOQWT010000283         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_58338_length_2158_cov_3.803614, whole genome shotgun sequence.
ACCESSION   DAOQWT010000283 DAOQWT010000000
VERSION     DAOQWT010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58338_length_2158_cov_3.803614"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /gene="livG"
                     /locus_tag="PKJ95_05895"
     CDS             <1..206
                     /gene="livG"
                     /locus_tag="PKJ95_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003968.1"
                     /note="Part of the ABC transporter complexes LivFGHMJ and
                     LivFGHMK involved in the high-affinity transport of
                     branched-chain amino acids; LivFGHMK is specific for the
                     transport of leucine, while LivFGHMJ is a transporter for
                     leucine, isoleucine, and valine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HNR65803.1"
                     /translation="MCFIQKIRDDFDLTILLIEHDMKVVMGICERIVVLNYGLTIAEG
                     NPKEIQHNPEVIKAYLGVEAQYA"
     gene            199..903
                     /locus_tag="PKJ95_05900"
     CDS             199..903
                     /locus_tag="PKJ95_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65804.1"
                     /translation="MLKVENLNVFYGGIHALKGVTFQVPKCKITTLIGANGAGKSTTL
                     RAICSLVKSQVGRITFQDKNITNIPTDDIVKMGVAMVPEGRKIFPNLSVKENLDLGAF
                     TREDKAEIARDLKWIYELFPRLKERLWQKGGTLSGGEQQMLAVARGLMSRPKLLMLDE
                     PSLGLAPLLVKDIIEVIQKICQEGITILLVEQNAFAALKISDYAYVLETGCLVLTGSG
                     EDLLNDAKVKKAYLGE"
     gene            930..1151
                     /locus_tag="PKJ95_05905"
     CDS             930..1151
                     /locus_tag="PKJ95_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006458055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65805.1"
                     /translation="MKFPVWLYGFIGLIGFFGFYGFVNNNPYELLFFTFFSFFAYFKF
                     LRRELEYLGLLGFVGLFVAILGIFGIIPV"
     gene            1420..>2158
                     /gene="pgsW"
                     /locus_tag="PKJ95_05910"
     CDS             1420..>2158
                     /gene="pgsW"
                     /locus_tag="PKJ95_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HNR65806.1"
                     /translation="MKNWIKYKVTITHLLLIFIILVSGLVLTEISKVKVPTGYSHLQY
                     QAAQIMLDSIEAISAGREEKGIPINVQLDPNKTGLIGEEYTILTTTLGNLSAKRTSTN
                     PDFAALMVKYFEQANLKAGDVIAVGSSGSFPALLMATFAACKATDITPIAIYAIGASE
                     YGATIPDFTFIDMLNILNQKNIINYKLKAVSMGGNQDQAEGMFFPDSKEVIESIAEQS
                     NITFINTGNLIANIGERLKIYQKEAGNK"
BASE COUNT          701 a          344 c          441 g          672 t
ORIGIN      
        1 ttatgtgttt tatacaaaaa ataagagatg attttgattt gaccattctt ttgattgaac
       61 atgatatgaa ggtggtaatg ggaatatgtg aaaggatagt tgtgttaaac tatggcttga
      121 ccatagcaga gggaaaccct aaggagatac agcataatcc agaggttatt aaagcttatc
      181 tgggggtgga ggcgcaatat gcttaaagta gaaaatctaa acgtctttta tggtggaata
      241 catgccttaa agggggttac ctttcaggta ccgaaatgta agatcacaac tcttatcgga
      301 gccaatggtg ccgggaaaag cactacctta agagccatat gttctttagt taaatcgcag
      361 gttgggagaa taacatttca ggataagaat attaccaata tcccaactga tgatattgtc
      421 aagatgggag tagcaatggt cccggaggga aggaaaatat tccccaacct gtcggtaaaa
      481 gaaaatttag acttaggtgc cttcacacgt gaagataagg ccgaaattgc tcgagatcta
      541 aaatggatct acgaattatt ccccaggtta aaggaaaggc tctggcaaaa aggaggaaca
      601 ctatccgggg gagaacagca gatgctggca gtggcaaggg gattaatgtc ccgtcccaaa
      661 cttttaatgt tagatgaacc ttctttgggg ctggcaccac ttttggtgaa agatataata
      721 gaggttattc aaaagatttg tcaggaagga ataaccattc tcctggtgga acagaacgcc
      781 tttgctgcct tgaaaatctc ggattatgcc tatgttctgg agaccggctg tctggtctta
      841 acaggtagtg gggaagacct gttaaacgat gccaaagtta aaaaggctta tctgggagaa
      901 taaaaaataa aaagaagggc agattaaaaa tgaaattccc ggtatggtta tatggattta
      961 ttggacttat aggttttttt gggttttatg gatttgttaa taataatccc tatgaattac
     1021 tattctttac tttcttttcc ttttttgctt attttaaatt tttacgccgg gagttagaat
     1081 atttgggttt gttaggattt gtgggattat ttgttgccat cctgggaata ttcggaatta
     1141 tcccagttta atcatcttaa atcttaaacc agaccgagaa taaacacctt ttttcaaggt
     1201 taataatata agttgtctga tatttaaaat caaaatatta ttacaggaaa agaacttatt
     1261 attaattttc agtcactatg acaaaccttt taatatcttt aaaagtatct tctcatcttt
     1321 ctgtttacct ctcactttgc tgtttccaca aataccaggc ctgttttaat aaagaattat
     1381 tagaggtagg aatattacca tttataaatt tgatataaaa tgaaaaactg gattaaatat
     1441 aaagtaacta ttacccattt gcttctgatt tttataattt tagtttcggg attagttttg
     1501 actgaaatat ctaaagtaaa ggtccccacc ggctattctc acctgcaata ccaggcagcc
     1561 caaattatgc tggattcaat agaagcaatc agtgcaggca gagaagaaaa aggaatacca
     1621 atcaacgtac aattagatcc caacaaaact ggtcttattg gtgaagaata taccatttta
     1681 accactactc tgggtaattt aagtgctaag aggacttcta ctaatcctga ttttgccgcc
     1741 ttgatggtaa aatattttga acaggctaat ttaaaagctg gtgatgtcat tgctgttggt
     1801 tccagtggtt catttccagc tctgctcatg gctacctttg cggcatgcaa ggctacagat
     1861 atcactccta ttgcaatcta tgccataggt gcatctgaat atggtgcaac catccccgat
     1921 tttaccttta ttgatatgct gaatatttta aatcagaaaa atattatcaa ttataaatta
     1981 aaagcagttt ctatgggagg gaatcaggat caggcagagg gaatgttttt cccggattcc
     2041 aaagaagtta ttgagagtat tgcagagcaa tctaatatta cttttatcaa tactggtaac
     2101 ctgatagcta atattggaga aagattaaaa atataccaga aagaggcagg gaataagc
//
LOCUS       DAOQWT010000284         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_58875_length_2147_cov_2.423996, whole genome shotgun sequence.
ACCESSION   DAOQWT010000284 DAOQWT010000000
VERSION     DAOQWT010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58875_length_2147_cov_2.423996"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..669
                     /locus_tag="PKJ95_05915"
     CDS             283..669
                     /locus_tag="PKJ95_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNR65807.1"
                     /translation="MVKCIATDKKYQCTIDLKSANYRLFLDTSKERGGLGDGISPHEL
                     LEAAAAACLNISIRIALDSLGIKADNVTVEVNLIRSVEGKSIFEYQYNIGDELNDEQK
                     EKIEESILSCPVRQTLLKEICYKRIE"
     gene            740..1204
                     /locus_tag="PKJ95_05920"
     CDS             740..1204
                     /locus_tag="PKJ95_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001992140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNR65808.1"
                     /translation="MKEYEQKLQKYIKDNNIECEYLKFEQSCHSVLEAARAAKVEPND
                     CVKNICLIDPEGKLIVAIVKGDDRVSTSRVGKALNTGKPRIATPEEILEKTGYPCGGT
                     PSFGYLAEFLIDPVVMEKEIILSGGGSEYTLIKIAPSELQKANKGRVIKIRK"
     gene            1213..1884
                     /locus_tag="PKJ95_05925"
     CDS             1213..1884
                     /locus_tag="PKJ95_05925"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNR65809.1"
                     /translation="MNKAIVRRITQVIFTLLLQGSLLFISAGTLRWNWAWILLATEII
                     ILLVNFVTLPGEVIKERGGEKENVKKWDKMLMSISVIPSLGIYITSGLDYRFSWSPYF
                     KSIINIISLIILLLSSILFTWSMVSNKFFSTMVRIQTERGHSIAVEGPYKYVRHPGYV
                     GYILMILATPVALGSLYALIASFLVMIIFIVRTVMEDKTLMSELEGYKEYSHRVKYRL
                     IPYLW"
     gene            1929..>2147
                     /locus_tag="PKJ95_05930"
     CDS             1929..>2147
                     /locus_tag="PKJ95_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR65810.1"
                     /translation="MIIKRLQDTPIMKTAHNVDARNLYDTENAVISVITLKPGQALKK
                     HITPVDVAFYVLRGKGVVEIGKEKQEVEA"
BASE COUNT          756 a          305 c          442 g          644 t
ORIGIN      
        1 aagtaataaa gcacactgcc tagcttggag ttgctataag aatttcctgc aactatccct
       61 catcccaaaa atagaatatt atctcgtccc ttgattagaa ttatcttaaa agaaattgaa
      121 attactgtag atgcctacca ggaaatgctt aataacttat agcttataca gaaaaaacaa
      181 tcttaatgtg aatatttttc aaatttttag tatactgttt actgtataat taaattattc
      241 tacgaaaaga aaaaatatta ataacaaaag gatgtagaaa taatggttaa atgtatcgcc
      301 acagataaaa agtatcaatg taccatagat ttaaaaagtg caaattacag gcttttcctg
      361 gacacctcta aagagagagg aggtttagga gatggaataa gcccgcatga attattagaa
      421 gcagccgcag ctgcctgttt gaatatttct ataaggatag cattagatag tttagggatt
      481 aaagcagata acgttaccgt tgaggttaat ctgataaggt ctgttgaagg taaatctatt
      541 tttgaatacc aatataatat tggtgatgaa ctaaatgatg aacaaaaaga aaaaatagaa
      601 gagtctattt taagttgccc ggttaggcag actctgctaa aagaaatatg ttataaaaga
      661 attgaatgat atctctcaat agaaggataa aacctgatta aagatagttt gaaacagaca
      721 attcatagag gaaaaagata tgaaagagta tgaacaaaaa ttacagaaat atattaaaga
      781 caataatatt gagtgcgaat atctgaaatt tgaacaatca tgccattcag ttttagaagc
      841 tgcaagggca gcaaaagtag aacctaatga ttgtgttaaa aatatctgcc tcattgatcc
      901 agagggaaag ttgatagtgg ctattgtgaa aggggatgac cgggtaagca cttcaagagt
      961 tgggaaggca ctgaatacag gcaagccgcg aattgctacc cctgaggaaa ttttagaaaa
     1021 aacaggctat ccttgcggtg gaaccccttc tttcggatat ctggcagaat tcctgattga
     1081 tcctgtggtt atggaaaaag aaattatttt aagtggtggt ggttctgaat acacgcttat
     1141 aaagattgca ccatcggagc tgcaaaaagc aaataagggc agagttatta aaatcaggaa
     1201 atgaattatc caatgaataa agctattgta agaaggatta cacaggttat cttcacctta
     1261 ctgctacaag gttctttgct ttttatttca gcaggaacgt tgcgctggaa ttgggcctgg
     1321 attctgctag cgactgaaat aattatttta ttggttaatt ttgtgacatt gcccggggaa
     1381 gtaattaaag aacgcggagg agaaaaggag aatgttaaaa aatgggataa gatgctgatg
     1441 agcatcagtg ttattccatc gctggggatt tatattacct caggtcttga ttatcgtttc
     1501 agctggagtc cctattttaa atccattatt aatattatta gtttaataat tttgttatta
     1561 tcttcaatac tctttacctg gtcaatggta tcaaataaat tcttttccac tatggtcaga
     1621 atccaaactg aacgaggtca cagcattgcc gtagaagggc cttataaata tgtgcggcat
     1681 ccgggatatg tcggttatat tttgatgata ctggcaacgc cggttgctct gggttcactc
     1741 tatgctttaa tagcatcctt tcttgttatg attattttta ttgttcgtac tgtaatggag
     1801 gataagactc taatgagtga actggaagga tataaagaat attctcatag ggtaaaatac
     1861 agattaattc catatttatg gtagaataat ttttagtaaa tgatgataga aattgggagg
     1921 aaaatattat gataatcaaa agattacagg acacacctat tatgaagact gcacataatg
     1981 tggatgccag aaatctctat gatactgaaa atgcagttat atcggttatc actttaaaac
     2041 caggacaggc tttaaagaaa cacattaccc cagtggatgt ggctttctat gttcttcgtg
     2101 gaaagggagt tgtggagata gggaaagaaa agcaggaagt tgaggca
//
LOCUS       DAOQWT010000285         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_58942_length_2145_cov_3.406220, whole genome shotgun sequence.
ACCESSION   DAOQWT010000285 DAOQWT010000000
VERSION     DAOQWT010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58942_length_2145_cov_3.406220"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="PKJ95_05935"
     CDS             <1..1821
                     /locus_tag="PKJ95_05935"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HNR65811.1"
                     /translation="DFRIPYLCQLPSGKSINIFFYNGSLAHSVSFGDLLKNGENFARQ
                     LMAGFSSDENRSELVHIATDGETFGHHHRFADMALSYCLNYIEENEFARITNYGEYLA
                     LNPPAYEVQIIKNTSWSCIHGIERWRDNCGCNTGAHPDWQQYWRKPLREAMDWLRDRA
                     IEIFEKGASAFFKDSWQARNEYIEVILDRCPNCIHDFLDKFSSHSLSVEEKVRAIKFL
                     ELQRNAMLMYASDGWFFDDISGIETVQNMRYAARCMQLINELSSINLEEEYITRLEEA
                     PSNIHRYRNGANIYNTMVKPAIIDLLRVGAHYAISALFNGKDKSIYTYQILDDKIERN
                     ENGQLKMAIGYATIRSEITLEEDKFCFAVLYLGGHNVNGGVINFKDEEFYLDMVKEFR
                     SSFNEANAVDIIRLMDKFFGTNSYTLWYMFRDEQRKVMEKILEPTLSSIESNYVHIYV
                     DNFPIINFLRQLNIPVPKSLSFITEEVLNQELKNVFSQKTIDLQKLNDLLQEIESWPI
                     EIDMITFEFNVVRWINRTIKKIRKNPFEIQPIIEITNVLKKMRDFEIKLNLWTAQNIF
                     FDIKKDISYALEKNMALQEEDREIWWQPVKELGEMLKISV"
     gene            1900..>2145
                     /locus_tag="PKJ95_05940"
     CDS             1900..>2145
                     /locus_tag="PKJ95_05940"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNR65812.1"
                     /translation="MNNRGSGLLIPIFCLPSRFGIGDLGLEAYRFADLLSKNNQHYWQ
                     ILPLNPTEIKHGNSPYHSCSAFAGNFILISPELLYRDG"
BASE COUNT          730 a          321 c          419 g          675 t
ORIGIN      
        1 gatttcagga ttccttatct atgccaatta ccatcaggaa aaagtattaa tatttttttc
       61 tataatggct ctttagctca ttcagtaagt tttggagact tgttaaagaa tggtgaaaac
      121 tttgctcgtc agttaatggc aggcttttcc tcagatgaaa atagatcaga attggttcat
      181 atcgctacag atggagagac ctttggccat caccatcgtt ttgcagatat ggctctttct
      241 tattgtttaa attacattga ggaaaatgaa tttgccagaa taaccaatta tggagaatat
      301 ttggcactaa acccaccagc ttatgaagta caaattatta aaaatacatc atggagctgc
      361 atacatggta ttgaacgctg gagggataat tgtggatgta atacaggagc acatccagat
      421 tggcaacaat actggcgaaa accgctgaga gaggctatgg actggttacg tgacagagct
      481 attgaaatct ttgagaaagg cgcttcagcc ttttttaagg atagctggca ggctagaaat
      541 gaatatattg aagtaattct tgatcgttgt cctaattgta ttcatgattt tttagataaa
      601 ttctcttcac attccctcag tgtagaagag aaggttagag ctatcaaatt tttggaattg
      661 cagcgcaatg ctatgctaat gtatgccagt gatggttggt tttttgatga tatatccgga
      721 atagaaacag tacagaatat gagatatgct gcccgttgta tgcaattaat aaatgaactt
      781 agttccatta atttagaaga agaatatata acgagattag aagaggcgcc aagcaatatt
      841 catcgctata gaaatggggc taatatttat aatacaatgg tcaaaccggc tattattgat
      901 ctgctccgtg taggagccca ttatgccatt tcggcactct tcaatggaaa agataaaagc
      961 atttatactt accaaatatt agatgataag attgaacgaa acgaaaatgg acagttaaaa
     1021 atggctattg gttatgcaac tattcgttca gaaattactc tggaagagga taagttctgt
     1081 tttgctgtac tttatttagg cggtcacaat gtgaatggag gggttataaa cttcaaagat
     1141 gaagaatttt atctggatat ggtgaaggaa ttcaggtctt cttttaatga agcaaatgca
     1201 gtagatatta taagattgat ggataaattt tttggtacaa atagttacac cctgtggtat
     1261 atgtttagag atgaacagag aaaagtaatg gaaaaaatat tggaacctac cctatcaagt
     1321 attgagagta actatgtcca tatttatgtt gataattttc ccattattaa ctttttaaga
     1381 caattaaaca taccggtacc aaaatctctt tcttttatta ctgaagaggt tctcaatcag
     1441 gaacttaaaa atgtattttc tcaaaaaaca atcgatttac aaaaacttaa tgatcttctt
     1501 caggaaattg aaagttggcc cattgagatt gatatgataa cttttgaatt taatgttgtg
     1561 agatggatta atcgaactat aaaaaagatt agaaaaaacc cttttgagat tcagcctata
     1621 attgaaatta ccaatgtatt gaagaagatg agagattttg aaattaaact caatctctgg
     1681 acagctcaga acatcttttt tgacatcaaa aaggatatta gctacgcact ggagaaaaac
     1741 atggcactcc aagaagaaga ccgggaaatc tggtggcagc ctgttaaaga attaggagaa
     1801 atgctcaaaa tcagcgtttg ataataaaaa tgtattaaaa tttttggaaa agaatacaaa
     1861 tttaaaaatt tttttatttt gagacgggga gtaaaagatg tgaataatcg aggcagcggt
     1921 ttgctaatac ctattttttg tctaccttcc agatttggaa taggagacct ggggttagag
     1981 gcttatcgat ttgccgattt attatctaag aataaccaac actactggca aatccttcct
     2041 ttaaatccga ctgagataaa acatggcaac tcaccatacc attcctgttc agcttttgct
     2101 ggcaatttca ttctaatcag tccggaactg ctctatcgag atggt
//
LOCUS       DAOQWT010000286         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_58944_length_2145_cov_3.338278, whole genome shotgun sequence.
ACCESSION   DAOQWT010000286 DAOQWT010000000
VERSION     DAOQWT010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58944_length_2145_cov_3.338278"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PKJ95_05945"
     CDS             complement(<1..1088)
                     /locus_tag="PKJ95_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65813.1"
                     /translation="MGSFSSLFFGRVSNNKPLQVIDNYSGIGFNPLFLGKVSNSKNMD
                     TVRLFQGYTLWPLRKLPKERLFFGKVIGGENSFSSMQTLSVVQPPTFKEKISIFGVYA
                     CVQFPDIGQYKVIPPADIGMVNLQTGTNCPIVWTMELCNFTGKYTHPDNEWTGIMDKG
                     KYHLARKTRKFLLIILCAKLGDGFCFLIFPRLVIKSKPVGLHILSMGGVDEISDILSN
                     KVSITSYVAEEALIKLSARKYLSTTLTRNEKSADTEVMVNYQNGTNSTIPTGETVVSN
                     SESFDLPTREQPNGTLTVFNLNISGTLDSITEAYYIQSNTQEEITLSGSISITGNKQI
                     TFSSAPEAIPNGSSLRIRGVQLTGTIVTP"
     gene            complement(1104..>2145)
                     /locus_tag="PKJ95_05950"
     CDS             complement(1104..>2145)
                     /locus_tag="PKJ95_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65814.1"
                     /translation="VRSVSETDSTYGKLTAIQERNKCFRFNGATGNFTDFSTGSITCM
                     QSTTDYMIFGVNEWTKNLYIEVDTPAIWTGFIWEYWNGTEWTSLPVDAVDGTSGLTVS
                     GNLFLGSITQTLWRKYYQNAYRMLWVRCKAPDSIDTPAVLSNAYWNYVYDTPLPFIKG
                     VGAYSDCDEAATAIYTPVTPAFEYPAMGRVVFDTDPLLGVGHTLRATYQAKNPPAGLY
                     SFTFQSCNSTTQTGQVIVNAGDPIGISFTPGQYMRNVIAGMIMAFAYDLADDNLANLT
                     ISRALKYLWFAEDAGATPTEFTNGDFTFASTIDPDEVNYFWIKYSPPVDYPTNENMIY
                     ANIYAVNTAVTI"
BASE COUNT          662 a          409 c          382 g          692 t
ORIGIN      
        1 ggtgttacta tagttccggt taattgaacc cctcttattc ttaaagaaga tccattagga
       61 atagcttcag gtgcagaact aaaggttatc tgcttattcc ccgtaataga tatagaaccg
      121 gaaagggtta tttcttcctg agtattgctc tgaatgtaat atgcttcagt aatactatct
      181 aatgtgcctg atatatttaa attaaatact gttaatgttc cattcggctg ttctcttgtg
      241 ggtagatcaa aactttcaga atttgaaact actgtttcac ctgtgggaat agttgagttt
      301 gttccatttt gataattaac cattacctct gtgtctgccg atttttcgtt tcttgtaaga
      361 gtagtggaaa gatattttct tgcagaaagt tttataaggg cttcttctgc tacgtaagaa
      421 gtaatggaaa ctttattact taatatgtct gaaatttcat caaccccacc cattgaaagt
      481 atatgtaacc ctacaggttt actctttatt accagtcgag gaaatattaa aaaacaaaat
      541 ccatctccaa gtttagcaca gaggatgatt aagaggaact ttcttgtttt acgggctaaa
      601 tgatattttc ctttatccat tatacctgtc cattcattgt caggatgggt atatttcccg
      661 gtaaaattgc aaagttccat tgtccataca atagggcagt ttgttccggt ctgtaaattt
      721 accattccta tgtcggcagg agggataact ttatattgcc caatgtcagg aaattgaaca
      781 caggcatata cgccgaaaat agaaatcttt tctttaaacg taggaggttg aaccacgctt
      841 aaagtttgca tagaggaaaa ggagttttcc cctccgatta ctttgccaaa aaataatctt
      901 tctttaggta gttttctcaa aggccatagg gtatatcctt gaaatagtct taccgtgtcc
      961 atatttttag aattacttac ttttccaaga aaaagaggat taaaccctat tccagaataa
     1021 ttatctatta cctgaagtgg cttgttgtta cttactcttc caaaaaaaag gcttgaaaaa
     1081 ctacccaact aatttcactc cctttaaatt gttactgccg tattaactgc atagatatta
     1141 gcataaatca tattctcgtt tgtgggataa tccacaggtg gagaatattt tatccagaag
     1201 taatttactt cgtcaggatc tatcgtggaa gcaaaggtaa aatctccatt ggtaaactct
     1261 gtaggagttg caccggcatc ttctgcgaac catagatatt ttaatgccct acttattgtg
     1321 agatttgcaa ggttatcatc tgctaaatca tacgcaaagg ccataatcat accggcaatt
     1381 acgtttctca tatactgacc aggggtaaaa gatattccaa taggatcgcc ggcattgact
     1441 atcacctgtc ccgtttgagt ggtagaatta caggattgga aagtaaaaga ataaagcccg
     1501 gcaggtggat ttttagcctg ataggttgcc cttaatgtat gccctactcc cagaagggga
     1561 tcagtatcaa aaactactct tcccattgca ggatattcaa aagccggagt tacgggagta
     1621 tatattgccg ttgccgcttc atcacaatct gaatatgccc ctactccctt tataaaagga
     1681 agaggggtat cataaacata attccaataa gcattggata aaaccgcagg agtatctata
     1741 ctatccggtg ctttacatct tacccagagc atacggtaag cattttgata atactttcgc
     1801 cataaagtct gagtaataga tccgaggaat agatttccag atacagttag accggaagtt
     1861 ccatcaacag catcaacagg taaagaagtc cattcagttc cattccaata ttcccatata
     1921 aatcctgtcc atatggcagg tgtatctact tctatataaa gattttttgt ccattcatta
     1981 acaccaaaaa tcatataatc agtagtgctt tgcatgcagg taatagatcc agtggaaaaa
     2041 tcagtaaaat ttcctgtagc accattaaat ctgaaacatt tatttctttc ctgaatggca
     2101 gttagttttc cataagtaga atctgtttca cttacagatc tgacc
//
LOCUS       DAOQWT010000287         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_58955_length_2145_cov_2.760287, whole genome shotgun sequence.
ACCESSION   DAOQWT010000287 DAOQWT010000000
VERSION     DAOQWT010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58955_length_2145_cov_2.760287"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PKJ95_05955"
     CDS             <1..779
                     /locus_tag="PKJ95_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNR65815.1"
                     /translation="YMSTLQYCMLAAAKYHKPIIVLDRPNLLGGIIVEGPVLEDNFKS
                     FVGVDNLPLAHGMTVGELAQFYNRKISADLTVIPMKGYHREMTFPETGLPFVQTSPNI
                     PDLNSVYGYMATGMGEGTGVFQREQFKWVGGKGLHSYQYAEILNKAELPGVWYIPEDR
                     DTAGGVCLDIYDPSLFNPAKSGIYALAYAFMLGDFQVPRSTPGNIVMFDKIMGTDKIG
                     FLLEEKTPPELIVEYYTPELERFKEERKNYLIPSYQPPCF"
     gene            complement(819..1646)
                     /locus_tag="PKJ95_05960"
     CDS             complement(819..1646)
                     /locus_tag="PKJ95_05960"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNR65816.1"
                     /translation="MSKELKKVKKAYNNLDFINSSDARVIRILAELLEPKQRFQKNNI
                     KDMIVFFGSARTMPPDEAENHLKNIEKKIKTSDGLDKTSLQEELENAKNQVFMSRYYQ
                     DAIELASRLTLWSKSLNSDSRFIVCSGGGLGIMEASNLGASKARGKSIGLNISIPMEQ
                     FFNKYITPELSFEFHYFFMRKFWFVYLTKGFVIFPGGYGTLDELFEVLTLLQTKKLKN
                     QIPIVIYGSEYWNQVINFDALVKYSTINKSDLNLFKICDTVEDAFFYLTEEITRLFL"
BASE COUNT          679 a          370 c          387 g          709 t
ORIGIN      
        1 cctatatgtc cactcttcaa tattgtatgt tagcagcggc aaaataccat aaacctatta
       61 ttgtattaga tagacctaac ctgctgggag gaataattgt tgaaggaccg gttttggagg
      121 ataatttcaa gtcttttgtg ggagttgata atctgcctct ggctcatggt atgactgtag
      181 gagaactggc acaattttat aatcgcaaga tttcagctga tttgactgtt atccctatga
      241 aaggatatca tcgggaaatg acctttcccg aaacagggct tccctttgtt cagacctcac
      301 ctaatatccc cgacctgaat tctgtctatg gttatatggc gactggaatg ggagaaggaa
      361 ctggagtttt tcaaagagaa caatttaaat gggttggagg caaagggctg cattcctatc
      421 aatatgctga aattctcaat aaggcagagc ttcccggagt atggtatatt cctgaagaca
      481 gggatacagc tggcggagtt tgtctggata tctatgaccc ttcacttttt aacccggcaa
      541 aaagtggtat ttatgcattg gcttatgctt ttatgctggg agatttccag gttcccagga
      601 gtactccagg aaatattgtt atgttcgata aaattatggg taccgataag attggctttc
      661 tgttagaaga aaagactcct cctgagctga tagttgagta ctacactccg gagttagaaa
      721 ggtttaaaga agagaggaag aattatctta ttccatccta tcaaccacct tgtttttaaa
      781 aattttttat tggaatgtgg tttaggatac tgaataatct ataaaaaaag tctggtaatt
      841 tcttcggtta aataaaagaa tgcatcctca accgtatcac aaatcttaaa caggttgagg
      901 tcagacttgt tgatagtgct atattttacc agagcatcga agttgattac ctgattccag
      961 tattctgagc cataaatgac aataggtatc tgattcttta atttttttgt ttggaggagt
     1021 gttaggactt caaacaattc atcaagtgtg ccgtaacccc cagggaaaat aacaaaccct
     1081 ttagtcaaat aaacaaacca gaatttacgc ataaaaaaat aatgaaattc aaagctaagt
     1141 tctggagtaa tatatttatt aaaaaattgt tccataggga tgctgatatt caagccaatt
     1201 gatttacccc gggctttgct tgcacccagg ttagatgctt ccataatccc cagtccgcca
     1261 ccagaacaga caataaagcg agaatctgaa ttcagtgatt tagaccagag agtcagacgg
     1321 ctggctaatt caatggcatc ctggtaatac ctggacataa aaacctgatt tttagcattt
     1381 tctaattctt cctgaaggct tgttttatcc aaaccatctg aggttttaat tttcttttca
     1441 atatttttaa gatgattttc tgcttcatcg ggaggcatag ttctggcaga accaaaaaaa
     1501 acaatcatat ctttaatgtt attcttttga aaacgttgct tgggttccag taattccgcc
     1561 aggattctta taaccctggc atcggaacta ttgataaaat ctaaattatt gtaggctttt
     1621 tttacctttt ttaattcttt actcatttgg attggcatac cttcctttaa atgatttgcc
     1681 ggtaaccata ctttctattc ccccctaata caatatcccc atttattata aaccattatg
     1741 aattaagatt tccagattat ttatctacct cgtttctatc aatcaactat ttatcaattt
     1801 tggctaaatt tatattgaaa atattaaagg gagtgtattt tattctgttc tgtggtatac
     1861 ttattctgaa attgaataat taattatatt tgataataag acattttata aaaaattaaa
     1921 taagtctacc ataaaaaaaa gaggcgatac aaagctgaat gtccattcaa ttttcctgag
     1981 gaagtcaagt ttatcccttt aaagttttta taaaaaacct gctatattac agcctttaac
     2041 tagcacggag tcagtattaa aaaaagagaa ttattttagg aggtgatgaa aagaagcaaa
     2101 ataatacacc ttgatttcat ttataattaa ctggatgagt agccc
//
LOCUS       DAOQWT010000288         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_59108_length_2141_cov_3.638543, whole genome shotgun sequence.
ACCESSION   DAOQWT010000288 DAOQWT010000000
VERSION     DAOQWT010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59108_length_2141_cov_3.638543"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..684)
                     /locus_tag="PKJ95_05965"
     CDS             complement(85..684)
                     /locus_tag="PKJ95_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65817.1"
                     /translation="MGYIKSALEIALEKTTGEKLTDKEIAEIKQQEKTDSILAKYYKG
                     QIEQEQLWHYFKELPVRYLRQAQNSFLRSLTFQSNVYDVEKRRAGILALENLKKPNRF
                     SDIEYYFEQIGKLQEELQKDKEELIKLVTKDLEKNPERRLQTFQQGNQIIIKELSLEE
                     VLEQDKGLKQKINQMEKKYKEKFKLWKEKISEFINENGE"
     gene            complement(690..1424)
                     /locus_tag="PKJ95_05970"
     CDS             complement(690..1424)
                     /locus_tag="PKJ95_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65818.1"
                     /translation="MDMDKRIRYAFEHSEILKWPKQLLSSFGSSEVHYHVLTEPVYQE
                     FARGEAETVVREGKVSWYRPQLLTPEYILRIEGFSDEAKKALLSIAGEMPDLASILYH
                     FHFKREVDSMNFVSGSIKSVADKIAREIDKRGDNLSTIIKGVGDLWDISLSKFILDMM
                     NRSARQSQFPELRKRGLVTYNNLGYQVVSRDAQGVPLIAKKEIETMFDMVKHGDMEPK
                     DLKAELDRWGLFEYYQDRFFELFRRR"
     gene            1704..>2141
                     /locus_tag="PKJ95_05975"
     CDS             1704..>2141
                     /locus_tag="PKJ95_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HNR65819.1"
                     /translation="MPIKKFIIGVMGGSQFVNPDDEQYAYQIGVMIAREGWILLNGGR
                     ASGIMEASAKGAKDNGGITIGILPVDDADWASQYIDIPIVTGIGMARNIINVLSSDMI
                     VALPGSAGTISEIAIALKYGKVVILFRFDAGDWLKPFQREGKVY"
BASE COUNT          628 a          401 c          375 g          737 t
ORIGIN      
        1 cgaacggcac gtacggtggt gtgagagggg gaagagattt ccccctactc gatcatatct
       61 taatggcaaa gggaaagagt tggtctattc accgttctca ttgataaatt cagaaatttt
      121 ttctttccac agcttgaatt tttctttata tttcttttcc atctggttta ttttttgttt
      181 taatccttta tcctgttcca gaacttcttc aagggataat tcctttataa ttatctgatt
      241 accctgttga aaagtctgta atcttctttc cggattcttt tccagatcct ttgttaccag
      301 tttaatcagt tcttctttat ctttttgcaa ttcctcctgc aattttccaa tctgctcaaa
      361 atagtattct atatctgaga aacgattggg ttttttcagg ttttccagag ctaaaatacc
      421 tgctcttctt ttctcaacat cataaacatt gctctggaag gttaaggacc ttaaaaagct
      481 attttgtgcc tgccttaaat aacggactgg caattcttta aaataatgcc acaattgttc
      541 ttgctcaatc tggcctttat aatactttgc caagatagaa tctgtctttt cctgttgctt
      601 aatttcagct atttccttat cggtcagttt ttcaccggtt gttttttcta aggcaatctc
      661 cagagcagac tttatataac ccattattgt tacctccttc taaacaattc aaaaaaacgg
      721 tcttgatagt attcaaatag cccccatcgg tctaattcag cttttaagtc tttcggttcc
      781 atatccccgt gttttaccat atcaaacatt gtttcaatct cttttttggc gataagaggg
      841 accccctgtg catcccgtga tacaacctga taacctaaat tgttgtaggt aaccaaccct
      901 cttttcctca gttcaggaaa ttgggattga cgggcacttc tgttcatcat atctaaaata
      961 aatttagata gagaaatatc ccaaagatca cccacacctt tgataattgt tgagagatta
     1021 tcacctcttt tatcaatttc acgggcaatc ttgtcggcaa ctgatttaat actgcctgag
     1081 acaaaattca tagaatcaac ttctctttta aagtgaaagt gatatagaat acttgccaga
     1141 tcaggcattt caccagctat ggatagaagt gcttttttgg cttcgtcgct gaatccctca
     1201 atacgcaaga tatattcggg agttaataac tgtggtcggt accagcttac cttgccctct
     1261 cttaccacag tttcggcctc tcctctggca aattcctgat aaacaggctc agtaagaaca
     1321 tgataatgaa cttctgacga accaaaggaa gagagcagct gctttggcca ttttaatatc
     1381 tccgaatgct caaaagcata tctaattctt ttgtccatat ccataacttc tacctctttc
     1441 agtaaaacat ttaaggtaaa tttattataa cccaatagtc tattatattt aatttgacct
     1501 gagtttccat cactaatatt ttaactaatt attttaaaat gtcaaatatg gagagccatt
     1561 atctgactgt taatttatta agaaaaattt attaaaaatc tatactgtta ctgattcctt
     1621 tcttaacttg agaaaataga atatttaagg tatataatag actaaggtag aaacaatact
     1681 gccaaaaata ttaagaggag gggattccta ttaaaaagtt tataattggt gtaatgggtg
     1741 gatcgcagtt tgtaaatcct gatgatgaac aatatgccta tcagattggg gtaatgatag
     1801 cccgggaggg atggatacta ttaaacggtg gcagggcatc aggtattatg gaagcatctg
     1861 ctaagggtgc taaagataat ggtggaataa ctataggaat attgccggtt gatgatgcag
     1921 actgggcttc tcaatacata gatattccta ttgttaccgg tataggtatg gccagaaata
     1981 ttattaatgt attaagcagt gatatgattg ttgccttgcc ggggagtgca ggaacaattt
     2041 ccgaaatagc catagcttta aaatatggta aagtggtaat cttatttaga tttgatgccg
     2101 gtgactggct taaaccattt cagagagaag ggaaagtgta t
//
LOCUS       DAOQWT010000289         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_59284_length_2137_cov_3.961095, whole genome shotgun sequence.
ACCESSION   DAOQWT010000289 DAOQWT010000000
VERSION     DAOQWT010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59284_length_2137_cov_3.961095"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKJ95_05980"
     CDS             complement(<1..386)
                     /locus_tag="PKJ95_05980"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNR65820.1"
                     /translation="MIKNRADVFGKEEDTIVAISTPPGIGGISIVRMSGPLSIEIASK
                     IFLYQGKVKKSPHEFHSHRIYYGTIIQPGEGALIDEVLLVVMRKPKTYTREDIVEINC
                     HGGYLVVNKVLEIVIKMGARIAEAGE"
     gene            complement(717..1226)
                     /locus_tag="PKJ95_05985"
     CDS             complement(717..1226)
                     /locus_tag="PKJ95_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNR65821.1"
                     /translation="MVDFENKEMEYKEQEEKALQILSRIAESINNSAEIRIVSSHHDY
                     TTFEISNTDPAIIIGKHGHTLDAIQYLVNLIVNKGNKEDNRRIYTVDIDGYRKRREES
                     LQKYAREKAEIVKKKGISIALYFMNSIERKLVHMALKEDSSVSTYSEGKEPYRRIIIK
                     PNPTEEKEE"
     gene            complement(1303..>2137)
                     /locus_tag="PKJ95_05990"
     CDS             complement(1303..>2137)
                     /locus_tag="PKJ95_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNR65822.1"
                     /translation="KLLSVLYGVTHNYGLAIILLTLLIRIVMYPLMKKQMISMQETQK
                     IQPLMQEIQKKYKDDKERMNQEVMRLYKEHGVNPMGGCLPLLVQMPILILFFRVLRDF
                     KYYIPNTEIIDAGFLWIKNLAEPDKLFGVFGNYSIGILPILVGVSMYFQQKLTMPPMP
                     ATSQSNSSSNPAANTQKMMGTIMPVMIAFISFSLPSGLSVYWFTSTLFDIVQKLIINK
                     KEPKIMKESENSPKKISQEKKGEQKREPESKQKESTPESSWIPGYEKLTKTPAKKEKP
                     Q"
BASE COUNT          593 a          407 c          311 g          826 t
ORIGIN      
        1 aattctccgg cttcagctat cctggcaccc attttaatta caatttctaa aactttattt
       61 accaccagat aaccgccgtg gcaattaatt tccacaatat cttccctggt ataagttttt
      121 ggtttacgca taacaaccaa gagaacttcg tctatcaggg caccttctcc gggttgaata
      181 atcgtaccat aataaatgcg atggctatga aattcatgtg ggctcttttt tactttcccc
      241 tgataaagga atattttact ggcaatctca attgaaagtg ggccgctcat ccgtacaatg
      301 ctgatacctc caatacccgg tggtgtagaa attgccacga tggtatcttc ttctttacca
      361 aacacatctg ctcgattctt tatcattgtt aatccattaa ttgtttctaa atttttaaac
      421 ttactttaat agccttactt tgctttacaa cctaattttt gattctgata taacaaaagt
      481 ttattgaaat taatattaag ttaaacaact atttttacac aatattatac ctgtatatta
      541 tgtaaaataa gataatattc agagaataaa aatattttct ttaaatattt attttcgggg
      601 ctttgaggaa tattttattt cccttagtag taattctcta acctgttttt gccagtattt
      661 tgaatatttt tttcattctg gttattgttt ttttctcaaa tcaaatactg gtattcttac
      721 tcctcttttt cttcggtagg gttcggtttg ataatgattc ttctatatgg ctctttcccc
      781 tcactataag tagagactga gctgtcttct ttaagagcca tatgcacaag ctttctttct
      841 atactattca taaaataaag agctatggaa atacctttct tttttactat ttctgctttt
      901 tctctggcat atttctgcag agattcttct cttcttttac ggtatccatc aatatctact
      961 gtataaatcc tacggttatc ctctttattt cctttattaa caatcagatt aaccaggtat
     1021 tgaatggcat caagggtgtg cccatgtttc ccgatgatta tagcaggatc tgtattgcta
     1081 atttcaaaag tcgtgtaatc atggtgagaa gagactattc ttatctctgc actattattt
     1141 atactttctg cgattctgga aagaatttgt aaggcttttt cctcttgctc tttatattcc
     1201 atttctttat tttcaaaatc taccaaaaga ctcctctttc taccaaaacc ttatcttctc
     1261 tttaaatatt taaatcctta aaacgaccaa tttaattaat ttttattgag gtttttcttt
     1321 tttggctggt gttttggtta gtttttcgta tcctggtatc caggaactct ccggtgtact
     1381 ctctttttgt ttgctctctg gttctctctt ctgttctccc ttcttctctt gacttatttt
     1441 ttttggagaa ttctcagatt ctttcataat tttaggctct tttttattaa tgataagttt
     1501 ttgaacaata tcaaaaaggg ttgaggtaaa ccagtatacg ctcaatcccg atggcaggct
     1561 aaaactaata aaagcgatca taacaggcat aatagtgccc atcatcttct gagtattggc
     1621 agcagggttt gatgagctgt tactctggct ggttgccggc atgggaggca ttgttaattt
     1681 ttgctgaaaa tacattgaga cacctaccaa aattggtaaa ataccaatac tataatttcc
     1741 aaaaacacca aatagtttat ctggttcagc taagtttttt atccataaga aaccggcatc
     1801 gataatctcg gtattcggaa tataatactt aaaatctctt aaaactctaa agaagaggat
     1861 taagatgggc atctggacta ataagggcag acatcctccc attggattga ccccatgctc
     1921 cttgtataac ctcattactt cctgattcat tctttctttg tcatctttat attttttttg
     1981 tatttcctgc atcagaggtt gaatcttctg tgtttcctgc atggaaatca tctgtttttt
     2041 catcagcgga tacattacta ttctaataag aagagttaat aaaattattg ccaatccata
     2101 attatgtgta acaccataca aaacactaag tagcttt
//
LOCUS       DAOQWT010000290         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_59446_length_2134_cov_2.686869, whole genome shotgun sequence.
ACCESSION   DAOQWT010000290 DAOQWT010000000
VERSION     DAOQWT010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59446_length_2134_cov_2.686869"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKJ95_05995"
     CDS             complement(<1..419)
                     /locus_tag="PKJ95_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNR65823.1"
                     /translation="MKIAQVYFTNLRAKPRNNLLDKLQNLVIRAGIRNIDFKSKFVAL
                     KIHFGEAGNLAYIRPNYVAPIVQMIKDAGGFPFLTDANTLYHGKRANAVDHLDTAMKN
                     GFNRIAVGCDVIIADGLKGTEYRKIKIDKKYCIAPMIG"
     gene            complement(497..1048)
                     /locus_tag="PKJ95_06000"
     CDS             complement(497..1048)
                     /locus_tag="PKJ95_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNR65824.1"
                     /translation="MKKPIEQKGEKKKSLFKYVNLTIVLVYSAFIAYSYLFKVSSGIR
                     MGTTLISFLSSMGKILPCAFIIIGLFEVWVPRERVEKHLGHQGGWKGYIWAIILAGTM
                     AGGLLVALPVSSALYHKGARLSVVFTFLGASAVVRIPMTLFEASFLGVEFTALRWLIS
                     IPLIIISSHFLEKYLTKKQFDIK"
     gene            complement(1045..1527)
                     /locus_tag="PKJ95_06005"
     CDS             complement(1045..1527)
                     /locus_tag="PKJ95_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNR65825.1"
                     /translation="MWFAFIAAVLLILSFCLDRPKTKKAVILAYKRFIFILPSFLIML
                     ILISVILFLFPQEKIIAYLNYGSNIVNIILAALIGSITAVPGFIAFPLAGILKEQGIS
                     YTIIASFTTTLMMVGTITFPVETTYLGKKVALIRNVIGFVIAIVVALLIGLYFGEIVL
                     "
     gene            complement(1874..>2134)
                     /locus_tag="PKJ95_06010"
     CDS             complement(1874..>2134)
                     /locus_tag="PKJ95_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008386084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65826.1"
                     /translation="IGWKGAIFATKVLACAGLDLLTDEARLAAVQKEFKDRMEGVDYH
                     PVIPADLWPPIPKENPADFKGPAVPNLPEPEKPESLLYWKTN"
BASE COUNT          727 a          428 c          333 g          646 t
ORIGIN      
        1 cctatcatgg gagctatgca atatttttta tcaatcttta tttttctata ttcagtacct
       61 tttaatccat ctgctatgat cacatcacac ccaacagcta ttcgattgaa accatttttc
      121 atagcagtgt caagatggtc aacagcatta gcccttttac catggtaaag ggtattggca
      181 tcagtaagaa aaggaaatcc accagcatcc tttatcatct gaacgatagg cgctacataa
      241 tttggcctga tataggcaag attgccagcc tctccaaaat gtatctttag ggcaacgaat
      301 ttacttttaa aatcaatatt cctaattcct gcccttataa ctaaattctg caatttatcc
      361 agcaaattat ttcttggttt tgcccttaag ttagtaaaat atacttgtgc tattttcatt
      421 catctgtacc ttcttctctt aataatataa atcgcaagac tcttcgttat catcgtcgtc
      481 ccctatttaa ctgctattat tttatatcaa attgcttttt ggttaaatac ttttccagaa
      541 aatgagagga aataatgatt aatggtatag agataagcca tctcagggcc gtaaattcaa
      601 ctcctaaaaa ggacgcttca aataaagtca tcggtattct aaccaccgca gaagctccca
      661 aaaaagtaaa aacaacactt aaccgtgccc ctttatgata taaggcagaa gataccggca
      721 aagccaccaa cagacctccg gccatagtcc ctgctaaaat aatggcccat atatatccct
      781 tccagccacc ctgatgcccc agatgttttt ctactctttc tctgggaacc cagacttcaa
      841 ataagccaat tataataaag gcacagggca atattttacc catagaagag aggaaagata
      901 tcaacgtagt acccattcta attccagaag acactttaaa aagataagaa taggcaatga
      961 aggctgaata gactaaaact atggtcaggt tcacatattt aaataaactc ttcttctttt
     1021 cccctttttg ttcaatcggt tttttcataa aacaatttct ccaaaataga gaccaatcaa
     1081 taatgctacc acaatggcaa ttacaaaccc tattacattg cggataaggg caactttttt
     1141 acccagatag gttgtttcaa caggaaaagt aatagtgcca accatcatta aggtggtggt
     1201 aaaggaggcg attatggtat aggatatacc ttgttcctta agtattcctg ctaaaggaaa
     1261 ggcaatgaag cccggaacag cagttattga accaatcagg gcggctaaaa taatatttac
     1321 tatattactg ccataattaa gataggcgat aatcttctcc tgaggaaaaa gaaacagaat
     1381 aacagaaatc aaaattagca taattagaaa agaaggcaga ataaagataa atctcttata
     1441 agctaaaatt actgcttttt tagttttagg gcggtccagg cagaaggata aaatcaataa
     1501 aacagcggca ataaatgcaa accacattat tcactccttt ttttattttc ctgtatttta
     1561 acctcaaaga ttaattaaat atgtataatc tttcagatgc aatgaacaaa atattcccca
     1621 tatttaaaaa cagtttttag aaaagcaatc atcttaaaca gattatatta tatattactg
     1681 caacaacttt gccaattaat atcaccactg tttgcctgaa tatgttatat tgaaggactt
     1741 atttcaaatg agatagaacc cttcaatttt aaaaataaaa aaaacaggca gaaggagaag
     1801 taataaaata attaaatcct tctgcctgaa tcttaattag ccagttcccc tattccgaaa
     1861 caattagttt aatctaattt gttttccaat agaggagcga ttctggtttt tctggttccg
     1921 gtagattagg tacagccggt cctttaaaat cggcaggatt ttcttttgga ataggtggcc
     1981 acagatctgc agggattacg gggtgataat ctaccccttc cattctatcc ttaaattcct
     2041 tttgaacagc agccaatctt gcctcatcgg ttaaaagatc aagaccagca caggccagca
     2101 ctttggtggc aaatatggca cccttccaac cgat
//
LOCUS       DAOQWT010000291         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_59491_length_2133_cov_2.845525, whole genome shotgun sequence.
ACCESSION   DAOQWT010000291 DAOQWT010000000
VERSION     DAOQWT010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59491_length_2133_cov_2.845525"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1313
                     /gene="alr"
                     /locus_tag="PKJ95_06015"
     CDS             132..1313
                     /gene="alr"
                     /locus_tag="PKJ95_06015"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNR65827.1"
                     /translation="MENILNSTWLEIDLDAVRLNIKNIKKHVGKQIKIMGIVKGNGYG
                     HDAVEISKIILEQGVDQLAVARIEEAIFLRKNNIQAPILVLGTALPEQFKSYINYQVM
                     ASISNLNDIYRFNDLAVKLNKQINLHLKMDTGMGRVGIKPEEIKEFYNNSGDKLSNTE
                     IVGIFTHFSTADEKDKSYTYHQFRIFMEIVGFIKDTNVSPMPCFHTANSGAILDLPET
                     WLDMVRPGCLIYGLYPSDEVNRTIKLYPALSFKSRITFIKKIPIGSFVGYGKTFQTKR
                     ETTVATLPVGYADGYPRTLSNFGQVLIRGELAPVIGRVCMDQMMVDVTDINNVAIGDE
                     VVLWGAQGNGNLAVQDIAKKINTVVDVIIHLTDKARVAKIFIKEGNPWKIKNILGEHY
                     F"
     gene            1297..1839
                     /gene="tsaE"
                     /locus_tag="PKJ95_06020"
     CDS             1297..1839
                     /gene="tsaE"
                     /locus_tag="PKJ95_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNR65828.1"
                     /translation="MSIIFKDRAENTILDQNNNNNIYLFISNKPEETIFLGMKIGNML
                     QKGDLIAINGDLGTGKTCLIKGIALGLKSDDCVTSPSFSIIKEYTANIPIFHFDFYRF
                     NKAEEIEELGYEEYFYGEGVTLIEWASKIKNYLPEEYLLINIFFANNYSFSRKIIFKP
                     KGERYKRMVKDLKSVEYIRN"
     gene            1820..>2133
                     /gene="tsaB"
                     /locus_tag="PKJ95_06025"
     CDS             1820..>2133
                     /gene="tsaB"
                     /locus_tag="PKJ95_06025"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNR65829.1"
                     /translation="MNILGIDTTAEVLNLAIINKGRFIVDYRINTAEKTHSALILPLF
                     KNMLSFTGIELKTVEGVAVSIGPGSFTGLRIGLSTAKGIAFSLSIPIIGINTLESYAF
                     RF"
BASE COUNT          753 a          301 c          404 g          675 t
ORIGIN      
        1 tagctacagc cattagcaaa aaaataagca atactcatct tactatttca catactaagc
       61 aattagcgat tgctcaggtt gtcattgaaa gtcaaatttt ataattttag agtaggcggt
      121 gatgggtaat aatggaaaat atactgaatt ctacctggct ggaaattgat ttagatgcag
      181 taaggctcaa cattaagaat attaaaaaac atgttggaaa acagataaaa ataatgggta
      241 ttgtaaaagg taatggatat ggacacgatg cagtggaaat ttctaaaata attttagagc
      301 aaggtgttga ccaacttgcc gttgcaagaa ttgaagaagc tatcttttta agaaagaata
      361 atattcaagc acctatatta gtgctcggta ccgccttgcc ggaacaattt aaatcatata
      421 taaattatca ggtaatggca tccataagca atttaaacga tatctataga tttaatgacc
      481 ttgctgtcaa attaaataaa caaataaatt tacatttgaa aatggatacc ggtatgggac
      541 gtgtggggat taaaccagag gaaatcaaag aattttataa taattctgga gacaaattgt
      601 ccaatacgga aattgtggga atattcactc acttttctac tgctgatgaa aaagataaga
      661 gttatacata tcatcaattc aggattttta tggagatagt aggttttata aaggatacta
      721 atgtttcacc aatgccttgt ttccatactg ccaacagtgg agcaattctt gatttacccg
      781 aaacttggct agatatggta cgcccaggtt gtctaatata tggtttatat ccatctgatg
      841 aagtcaatag aacaataaag ctttatcccg ctttatcttt taaaagtcgt ataactttta
      901 ttaaaaaaat accaataggc agttttgttg gttacggtaa gacctttcaa acaaaaagag
      961 aaaccactgt ggctactcta ccggtaggtt acgctgacgg gtatcctcga actctttcca
     1021 attttggtca agtgttaata agaggtgagt tggcacccgt aattggcaga gtctgtatgg
     1081 accagatgat ggtagatgtt accgatataa ataatgtggc tattggtgat gaagttgttc
     1141 tctggggagc acagggaaac ggcaatttgg ctgtccagga tattgccaaa aagattaata
     1201 ccgtagttga tgtaatcatt catctaactg acaaagccag agttgcaaaa atatttatca
     1261 aagaaggtaa tccttggaaa attaagaata ttttaggtga gcattatttt taaagataga
     1321 gctgaaaata caattttaga tcagaataat aataataata tatatctttt tatttctaac
     1381 aagccggaag aaacaatatt tctgggaatg aaaataggaa atatgcttca gaagggtgat
     1441 ttaattgcca taaatggaga ccttggtact ggaaaaacct gcctaattaa aggtattgct
     1501 ctgggcttga agagtgatga ctgtgttacc agtccttcct tttccatcat taaagaatat
     1561 actgctaata tccccatttt tcattttgac ttttacagat ttaataaagc ggaagaaata
     1621 gaagagctgg gctatgaaga atacttttat ggagagggag ttaccttaat tgagtgggcc
     1681 agtaaaataa aaaattattt accagaagaa tatttattaa taaatatatt ttttgctaac
     1741 aattatagct ttagtagaaa gattattttt aaacctaaag gtgaaagata taaaagaatg
     1801 gtgaaggact taaaatctgt tgaatatatt aggaattgat acaactgctg aggtgctaaa
     1861 cctggcaata ataaataaag gtagatttat agttgactat agaataaata cggcagagaa
     1921 aacccattca gcccttatcc tacctctttt taaaaatatg cttagtttta ctgggattga
     1981 acttaaaaca gttgaaggag ttgctgtttc cattggtcca ggttctttta caggtttaag
     2041 aataggactt tctactgcca aaggaattgc tttttcttta tctattccca ttattggaat
     2101 aaatactttg gaatcatacg cttttagatt tat
//
LOCUS       DAOQWT010000292         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_59983_length_2122_cov_2.989840, whole genome shotgun sequence.
ACCESSION   DAOQWT010000292 DAOQWT010000000
VERSION     DAOQWT010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59983_length_2122_cov_2.989840"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1871)
                     /locus_tag="PKJ95_06030"
     CDS             complement(219..1871)
                     /locus_tag="PKJ95_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNR65830.1"
                     /translation="MVKKNNFNRFFIVIITVVVVLTFLSVIGWANEAENNGNYGLWSL
                     VPPILAIILCIILKEALVSLLIAVVVGTTILNNGNLWTALEKTANILVEQVADSWNAS
                     IILFFFLVGGLMGMIFFSGGGQAFLESISKKANNSKMAQIFAWLGGIVIFIDDYANSA
                     FIGSLFRPLSDKYNVSREKLAYIVDSTAAPVSSIFLISTWIGYQVGLIGESLPEGYNV
                     SPYFLWFKSIPYNFYSILAIIMVGIIACTGRDFGPMLKAEHRARTTKELWAKEARPLA
                     GTSDLKVANNASSNSMNLWVPIILLIVLSFYFMWASGGGSSAESFSQAISDADSMFSI
                     FLATLIAFFVAIIMYLMQKIATVAEIMDSFLNGARMMVYATLILVSAWAIKAVCDELG
                     TAPYIVNSLEGILSPLILPILTFLVSAFIALCTGTSWGTMGIVVPIVIPLGISVGTPL
                     PIVISSVLTGAVMGDHCSPISDTTVMSSTFAGSDHIDHVRTQFPYALTVTLIAVICYL
                     LAGLGIPVIVVLPVGAVLLYLLIYVFSSISAKQMGITFPLEK"
BASE COUNT          733 a          406 c          330 g          653 t
ORIGIN      
        1 atctggcgat aattaactcc ccttttaggt gacattttct aaagttattg acattttctt
       61 tttgtgataa tatgctgcaa aaccgtttta ttatgaaaac aatattttta ataaaaaggc
      121 aatccaccat tctatcttta ttattgaaag atcattatga tagattgcct taatgatatt
      181 ttatttaatt acctgttaaa tattttactc ttatggctct acttttctag aggaaatgtt
      241 atgcccatct gttttgctga aatagatgag aaaacatata tgagcaggta aagcaataca
      301 gccccaacag gtaatacaac aattactggg attcccaatc ctgctaatag atagcagata
      361 actgcaatta atgtaacagt aagtgcatag gggaattggg ttctaacgtg atctatatga
      421 tcagagccgg caaaagtaga agacataaca gtagtatccg agatagggct acagtgatct
      481 cccattactg cacctgttag tacactggaa ataactattg gtaatggggt acctacacta
      541 attccgagag gaataactat tggcactaca atacccatag ttccccatga agtacctgta
      601 cataatgcaa taaaggccga aacaaggaat gtcagaattg gtaaaattaa gggtgataaa
      661 attccttcta acgaatttac aatatagggc gcagttccca gctcatcaca tactgcttta
      721 attgcccagg cagaaactaa tattagtgta gcgtagacca tcattctggc tccattaaga
      781 aatgaatcca ttatctcggc tactgtggca attttttgca ttaaatacat tattattgcc
      841 acaaaaaagg caatcagagt tgcaagaaaa atggaaaaca tagagtcagc atctgaaatt
      901 gcctgcgaaa aagattctgc tgaacttccc ccaccgcttg cccacataaa gtagaaagat
      961 agtactatga gcaaaataat aggtacccag agattcattg aattacttga ggcattatta
     1021 gctactttca gatctgatgt tccagccaga ggtcttgctt ctttagccca tagctccttt
     1081 gtggttctag cacgatgctc tgctttcaac atgggaccaa aatctcttcc tgtacaagca
     1141 ataatgccaa ccataataat agctaaaata gaataaaaat tgtaaggaat gctcttaaac
     1201 cataaaaaat atggtgatac gttatatcct tctggtaaag attctcctat caagccaacc
     1261 tgataaccta tccaggtcga tattaaaaat attgaagaaa ccggtgcagc ggtagaatcg
     1321 acaatgtatg ccagtttttc ccttgaaaca ttatatttat ctgataatgg tcgaaatagg
     1381 cttccaataa aggcagagtt agcataatca tcgataaaaa ttacaatgcc acctaaccag
     1441 gcaaaaatct gtgccatctt cgaattatta gctttcttgc taattgattc caaaaatgcc
     1501 tgccctccac cggagaaaaa tatcataccc atcaaaccgc ccactaaaaa aaagaataag
     1561 ataatacttg cattccagga atcggcaacc tgttcaacca atatatttgc tgtcttctcc
     1621 aaagcagtcc ataaattacc attgtttaaa attgtagtgc cgactaccac agctatcaat
     1681 agtgacacta atgcctcttt taaaataata caaagtatga ttgctaaaat tggcggtact
     1741 aatgaccaca agccataatt tccgttattt tctgcctcat ttgcccagcc gattacagac
     1801 aaaaatgtta aaacaacaac tacagtaatg attacgataa aaaatctatt aaaattattt
     1861 ttctttacca ttattttaaa aaacactcct ttataatatt tttttatcac ttaattaaaa
     1921 ttatttaatt atttgtatta ctttcatgat gtgtttacac attgctgttc tttcctatag
     1981 aaataggata ctatattaac caggttccgg taaagataat tttcgcatac catttaataa
     2041 tttctgatag ggaatctttc cattcaaccg gtaaccctga ttcgttcttt taaaattgta
     2101 atagtagata aactgatcca ga
//
LOCUS       DAOQWT010000293         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_60322_length_2114_cov_3.374939, whole genome shotgun sequence.
ACCESSION   DAOQWT010000293 DAOQWT010000000
VERSION     DAOQWT010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60322_length_2114_cov_3.374939"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1007..1594
                     /locus_tag="PKJ95_06035"
     CDS             1007..1594
                     /locus_tag="PKJ95_06035"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNR65831.1"
                     /translation="MNTPVTEFTIEELADTIRKCQKCHLSRTRKMAVPGEGNPNSIVM
                     FVGEAPGGDEDREGLPFIGRAGQLFTRILQSVQIDRKSVFITNIVKCRPPENRNPNKS
                     EIDLCLPYLESQIALINPKIIVTLGSVPAKCLLNSEEAISRLRGKWFSWTGGIKIFPM
                     FHPSYLLRHQETTPGSPKELTWRDIQELKRVLDTF"
     gene            1659..>2114
                     /locus_tag="PKJ95_06040"
     CDS             1659..>2114
                     /locus_tag="PKJ95_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNR65832.1"
                     /translation="MKIRELINIIERIAPFFLQQSFDNSGVQYADLDEKIKKVLICLD
                     VTIETIEEALKQNCNTILSHHPLFFQPLTKITKQDNPTTFQLTHHHINLISAHTNYDL
                     AENGLNDYVGRLLGLKKIDSLEPSSEKILKLALYVPEKYQDNLLNALFKA"
BASE COUNT          716 a          345 c          372 g          681 t
ORIGIN      
        1 aaatgcactt tttaaccgta ggggcgaaac ggtttctttg aaggcatggg ctgatagttg
       61 tagtggtgat gaatttatgc ccgagtattg ccgagtggaa gaaagcaatg aaaataataa
      121 tggatcagca tcaataacag tggtattgcc ttaacactaa cctaatatag ctaaaaaaag
      181 taaaagatat gaggaagagg tgttagataa tttgaccgat attgatgatt ggattgacac
      241 ctcttctgtt ttgtattttt tctgctactt tataataatg gtcgaaatga atctctatgc
      301 ggcagagtta gggtgtgttt aaattcataa aacaaattat ttagctaatg cataactttt
      361 tatatccttt ttcttggtca tacacatatt tacttgatag tgttgataaa aaactagctg
      421 accaaaattc tcctcccttt atttcccgtt tacttgtgga tttttaacaa atatcttttt
      481 aatagttata ctttttatta ttcttatgat ctaggtcaca ccatagcttg gcactgatgt
      541 attacaaaat ggtatgattt tagcttattt ctatttctag gaacgtatgt catatctctt
      601 ggaaatacct atcttcactt ctctattatt taatatggaa tatagatctg aacatagatt
      661 tagatagttc cctattatca tatcacaaag aataggacat agaaatacag caataatgta
      721 attggatcgg tatgagcagg tatttaacat tttatttcat tattccactg aggcattcga
      781 acaatgtaaa gaagctgagt ttccttgttt attgagcttc accgtgataa aatattaaat
      841 tgagataggt cctaaagata cttttgttgc tcaattcatt taaagattaa taaataataa
      901 aaatataaaa accaattaat gaatgtgagc cctgcataaa aatatctaag taatctgcta
      961 tagatatttt acttactttt gaatatctga tagtagttga ggttttatga atacacctgt
     1021 aactgaattt accattgaag agcttgctga tacaattaga aaatgtcaaa aatgtcatct
     1081 atcccgtact agaaaaatgg cagttcccgg agaaggcaat cccaacagta ttgtgatgtt
     1141 tgtaggagaa gcccccggag gggacgaaga tagagaaggt cttcctttta taggtagagc
     1201 cgggcagctc tttacccgta tcttacaatc tgttcaaata gacagaaaga gtgtattcat
     1261 taccaatata gttaaatgta gaccaccgga aaatagaaat cccaataaaa gtgaaattga
     1321 tctttgttta ccctatctgg agagccagat agccttaata aaccccaaga ttattgtaac
     1381 tctgggtagc gtaccagcta agtgtctctt aaattcagaa gaagcaattt cccgtcttag
     1441 aggcaagtgg ttttcctgga caggaggaat caagattttt cctatgtttc accccagcta
     1501 tcttctgagg catcaggaaa ctacacctgg cagccctaaa gaactgacct ggcgggatat
     1561 ccaggaactt aaaagagttt tagatacatt ttaaaaattg ccagccaaac aaagatgatt
     1621 aaatctgttt ttaaagaaag gaatgggggc attctgatat gaaaataaga gaactgataa
     1681 atatcattga acggattgca cccttttttt tgcagcagag ttttgataac agtggggtac
     1741 aatatgctga tcttgatgaa aaaataaaga aagtattaat atgtcttgac gttaccatag
     1801 aaacaataga agaggcttta aagcagaatt gtaacactat actttctcat catcctttgt
     1861 tcttccaacc ccttacaaag attaccaaac aggataaccc aacaactttc caattaaccc
     1921 atcaccacat aaatctaatt tctgcgcata ctaattatga tctggcagaa aatggtttaa
     1981 acgattatgt gggtagattg ctcggtttaa aaaaaattga ttctttagag ccatcttcag
     2041 aaaaaatatt aaagctggct ttatatgttc ctgaaaaata tcaggataac cttttaaatg
     2101 cccttttcaa agct
//
LOCUS       DAOQWT010000294         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_60860_length_2103_cov_2.536621, whole genome shotgun sequence.
ACCESSION   DAOQWT010000294 DAOQWT010000000
VERSION     DAOQWT010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60860_length_2103_cov_2.536621"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PKJ95_06045"
     CDS             complement(<1..527)
                     /locus_tag="PKJ95_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65833.1"
                     /translation="MNRKNISLVFKKEIQGAIRDRRTLISMIVVPLIFYPLLFLGIGY
                     FSVIGQRNSENIPSLISITGAENSPELIEILKKDKNITIIHSENNLDDKLVADNIQLT
                     IDIPEQIEIERDYRENNKIIPLSLNMHYDSTAQNSLVAKKRVSLLIDAFRRKIIEERL
                     NKIGLNSDFLEPFLE"
     gene            complement(524..1273)
                     /locus_tag="PKJ95_06050"
     CDS             complement(524..1273)
                     /locus_tag="PKJ95_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR65834.1"
                     /translation="MIKVHHLKKEFFDKKGGKIEAVNDINFHCLKSQIFGLLGPNGAG
                     KTTTLRILATMMKPTAGEALVNGYQVVKEAHKVRQSIGFLSGETGLYDRFTPRETILF
                     FGRINGLSEKLISTRMSDIFKILDMESFKDVRVDKLSTGMKQKLSIARAIIHNPPILI
                     LDEPTAGLDVITARVVVNYIKEFRNQGKCIIFSTHQMAEAEKLCDEIAIIHKGKILSS
                     GTLAELQAKFQEQDLEEIFFKLVKVKAEGEV"
     gene            complement(1606..2088)
                     /locus_tag="PKJ95_06055"
     CDS             complement(1606..2088)
                     /locus_tag="PKJ95_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HNR65835.1"
                     /translation="MEIKVVKLEYPENTNIILGQSHFIKTAEDLYEIMVSSVPGVKFG
                     LAFNEASGVCLIRAEGNDHKLKTLAISNIKGIGAGHAFIIIMENAFPINVLNAVKQCP
                     EVCRIFCATANPVEVVVAETKQGRGILGVIDGLTPKGVEGEEDVEVRKKFLRTINYKL
                     "
BASE COUNT          615 a          439 c          323 g          726 t
ORIGIN      
        1 tgctccagga atggttctaa aaaatcgcta ttgaggccta tcttattcag cctttcctca
       61 attatttttc tgcgaaaagc atctatcaat agacttaccc tttttttagc aactaaggaa
      121 ttttgcgcag tagaatcata gtgcatatta agagagaggg gaattatttt gttattctct
      181 ctataatctc tctcgatctc aatttgttca ggaatatcta tagttagttg aatattatcc
      241 gctacaagct tatcatctaa attattctcg gaatgaatta ttgttatatt tttatccttc
      301 tttaatattt caatcaattc cggtgaattt tctgccccgg ttatactgat aagagaagga
      361 atattttctg aatttctctg gcctattacc gaaaaataac ctatacctag aaaaagaaga
      421 gggtagaaga tgagaggtac aacaatcata ctgattaatg ttctacggtc acggatggca
      481 ccctgtatct cttttttaaa taccaggctt atattttttc gattcatacc tccccctccg
      541 ctttaacttt gaccagttta aaaaatatct cttccaaatc ctgttcctga aactttgcct
      601 gtagttctgc cagggtgcct gaggaaagta ttttaccttt atggataata gcaatttcat
      661 cacatagttt ctccgcctct gccatctgat gggtggagaa aataatacat ttcccctgat
      721 tcctgaattc cttaatatag ttgactacca ctctggcagt tataacatcc agtcctgctg
      781 ttggttcatc taagattaaa atggggggat tatgaataat ggcccgggca atagataact
      841 tctgtttcat accagtggaa agtttgtcaa ccctgacatc cttaaaactt tccatatcta
      901 aaattttaaa aatatcactc attctggtac tgattaattt ttcagataaa ccattgattc
      961 taccaaagaa gagtatggtc tctctcgggg taaaacggtc gtacaaccca gtttccccgg
     1021 aaagaaaacc tatgctctgt ctcactttat gggcttcttt taccacctga tagccgttaa
     1081 ccagagcctc tcctgcggtc ggcttcatca tggtggccag aatacgcaag gtggtggtct
     1141 tgcctgcccc gttaggtcct aataagccaa agatctggct tttgaggcaa tgaaaattaa
     1201 tatcattaac cgcctcaatt ttaccccctt ttttatcgaa aaattctttc tttaaatggt
     1261 gaactttaat caagaaaagc cctttctttt ataatagtcg ttagtaataa agtttattgt
     1321 ttttagttaa tggtttttag taaagaattt ttaattaaaa ataatgcttc taaccagtat
     1381 aacctgaatt tattctttgt taataatttt caactttaaa ctgtgaacct tacttttttg
     1441 tcattgcgaa cctgcctctt agctttagct aagggcaggt gtggcaatca cattcactta
     1501 tacccacact ctttattatt atattttgac taaaaacgta aaaatataaa caatgaacta
     1561 aaaaccatat actcacagtt cttataatat aatatgcaaa aatttttata acttatagtt
     1621 aatggtcctt aaaaacttct ttctaacctc tacatcttcc tccccctcaa ctcctttagg
     1681 agtcaaaccg tcaatgactc ccaggatgcc ccgcccctgc tttgtttctg ctactactac
     1741 ttcgaccgga ttggcagtgg cacaaaatat ccggcacact tccggacatt gtttaacagc
     1801 attcagtaca ttaatgggga aggcattttc cataataata atgaaagcat ggcctgctcc
     1861 tattcctttt atattagaaa tagccagggt ttttaattta tggtcatttc cttcagccct
     1921 gatcagacat acccccgatg cctcattgaa ggctaatcca aatttaactc caggcacaga
     1981 agaaaccatt atttcataaa ggtcttctgc tgtcttaata aaatgagatt gaccgagtat
     2041 gatattagta ttctccggat attctaactt aactacttta atctccattt ttgctctcct
     2101 ttt
//
LOCUS       DAOQWT010000295         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_61051_length_2098_cov_2.997063, whole genome shotgun sequence.
ACCESSION   DAOQWT010000295 DAOQWT010000000
VERSION     DAOQWT010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61051_length_2098_cov_2.997063"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1348
                     /gene="ugpC"
                     /locus_tag="PKJ95_06060"
     CDS             248..1348
                     /gene="ugpC"
                     /locus_tag="PKJ95_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNR65836.1"
                     /translation="MARVVLKNVTKKFKDVVAVDNISIDIEDKEFAVLVGPSGCGKST
                     TLRAIAGLEEVTSGEIYIGDRLVNDIPPKDRDIAMVFQNYALYPHMDVYNNMAFGLKL
                     RKYPKAEIDERVHEAAAILGIEKLLKRRPKELSGGQRQRVALGRAIVRKPKVFLFDEP
                     LSNLDAKLRVAMRGEISKLHNRLEATIIYVTHDQIEAMTMASRIFILNEGRLQQEGPP
                     LDVYQMPGNQFVAGFIGSPAMNFFSSTITGKDSSYFIDTDSFRVKLPPQFSKAISSYD
                     GKKVTFGVRPEDFHDREFYPEANSDNTIKVKIELIEPLGDEVLFYLVSGKYQIVAKLD
                     SRTRKKVGEEMEIALEVAKTHIFNPENEETLV"
     gene            1507..>2098
                     /locus_tag="PKJ95_06065"
     CDS             1507..>2098
                     /locus_tag="PKJ95_06065"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNR65837.1"
                     /translation="MEDNLKKYFVEMVKIDSESGEEQEFLKALESLFQRELNAKAEYD
                     SYGNLILRVKGRNSNKKEPLLFCCHADTVKPGKGIETVLENGIIRSKGETILGADDKA
                     GISEILVALQKASQYPPVEILITREEERGLLGSKFLDRSLLKSKKGYVLDSEALQEII
                     IGGPSRVEMTIRIIGKAAHAVEPEYGISAIEVAAQAI"
BASE COUNT          705 a          368 c          450 g          575 t
ORIGIN      
        1 ataaatacgt tcaatcttag accagagaag aaattggaca agcacgctta agttttaaac
       61 atattacgcc tgtgaaaatg tggtaatcga tatacagtat agtttactat aatagaaaaa
      121 agattcaatt cctttttaag tcttactgtt tataaatata tgataagatg aaattaatac
      181 aagttcttaa ataaagattg gattagataa gaagagacca tttttttaat ggaaaaagga
      241 ggcttttatg gctagagttg tcttaaaaaa tgttaccaaa aagtttaaag atgttgtagc
      301 ggtagacaat atcagcattg atattgaaga taaagagttc gcagtactgg taggaccctc
      361 tggatgtggc aagagcacta ccctgcgagc catagcagga ctggaagagg taacctcagg
      421 tgagatttac ataggagacc gcctggtcaa tgatattcca cccaaagatc gtgatatcgc
      481 catggtcttt caaaattatg ccctctatcc ccatatggat gtctacaaca atatggcttt
      541 tggcttaaaa ctgcgtaaat atcccaaagc agaaatagat gagcgagtcc atgaagcagc
      601 agctatttta ggtatcgaaa aactgctcaa aagacgcccc aaagagctct ccggaggtca
      661 gcgtcagcgt gtagccttag gtcgtgccat agtgcgtaaa cctaaagttt ttctcttcga
      721 tgaaccgctc tccaacttag atgccaaact tcgtgtggct atgcgcggtg agatatccaa
      781 gcttcataat cgcctggagg caactatcat atatgttacc catgaccaga tagaagcgat
      841 gactatggct tcccgtatct ttattcttaa tgaaggccga ctgcaacagg aaggcccgcc
      901 tttggatgtc tatcaaatgc caggtaatca gtttgttgcc ggtttcattg gttctcccgc
      961 tatgaatttc ttctccagta ctattaccgg gaaagactct tcttacttta tcgatacaga
     1021 tagttttcgg gttaaattgc ctcctcaatt tagtaaagcc atctcctcat atgatgggaa
     1081 aaaagtcacc ttcggggtac gccctgaaga cttccatgac agagagtttt atccagaagc
     1141 caacagtgat aacaccatta aagtaaaaat tgagcttatt gagccactgg gagatgaggt
     1201 acttttctac ctggtttccg gtaagtacca gattgtagcc aagctggata gcagaaccag
     1261 aaaaaaggta ggggaggaga tggagatagc tctggaagtt gccaagactc atatctttaa
     1321 ccctgaaaac gaagaaacat tagtgtaagc accttatata cttttaatat tttttgagta
     1381 cacataccta atactaaaac aagaacagta attatacagt tcttaataaa acaagtaatt
     1441 gttaatttat tacctatttt agataaatga aataatttaa gataccaata gttggaagga
     1501 gagacaatgg aagacaatct gaaaaagtat tttgtggaaa tggtgaaaat tgatagtgaa
     1561 tcaggggaag agcaggaatt tttgaaagcc ctggaaagtt tattccaacg agaactgaat
     1621 gctaaagcag aatatgacag ctatggaaat ttaattttaa gagttaaggg caggaatagc
     1681 aacaaaaaag agcccctctt attttgttgc catgctgata cagtaaagcc cggcaaagga
     1741 attgaaacgg tgctggaaaa tgggataatt cgttcaaaag gagaaaccat attaggagca
     1801 gatgacaagg cgggtattag tgaaatatta gtagcattac aaaaagcctc tcaatatcct
     1861 cccgtagaga ttctgataac tcgtgaagag gaaagagggt tgcttggttc caaatttttg
     1921 gatagaagtc tattgaagtc aaaaaaaggg tatgttcttg attcggaagc tttacaagaa
     1981 ataataatcg gagggccttc ccgtgtagaa atgactatca gaattatcgg aaaggctgcc
     2041 catgcggtag aaccagaata tggcatctct gccatagaag ttgctgcaca ggctatct
//
LOCUS       DAOQWT010000296         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_61387_length_2091_cov_2.796660, whole genome shotgun sequence.
ACCESSION   DAOQWT010000296 DAOQWT010000000
VERSION     DAOQWT010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61387_length_2091_cov_2.796660"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1836)
                     /gene="tkt"
                     /locus_tag="PKJ95_06070"
     CDS             complement(<1..1836)
                     /gene="tkt"
                     /locus_tag="PKJ95_06070"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNR65838.1"
                     /translation="MLEEVAKIIRGLSVDAIEKANSGHPGLPLGCADLGAVLFAEILK
                     HDPEKPDWLDRDRFILSAGHGSMLLYALLHLSGYDITIEDIMNFRQIGSKTPGHPEYR
                     HTPGVEVTTGPLGQGFANGVGMALAEKILAEKFNEKDFDIVNHYTYVLASDGCMMEGI
                     TSEAASLAGHWGLEKLIVIYDQNQISLAGRTDLTFTESVAERYRAYNWRVIGNVNGHD
                     FDELKGALMKAREKSDKPVLIIVQTHIGFGAPTKQDSHTSHGAPLGHEEVIGLKKNLG
                     LPADKTFYVSNEVREFFEKRKSELRQLRRQWDDKFISWSEKFPEKRRQLDESIDLKVP
                     EEMGFWKMDLAEKIATRSSSGEILNKIAEEIPYIIGGSADLEPSTMTYLEKFSELQKN
                     CYNARNIRFGVREHAMGAICNGLALHKGIRPYCSTFLVFSDYMRPAIRLAALMNLPVI
                     FIFTHDSVYVGEDGPTHQPIEQLESLRIIPNLQVIRPADNDEVKVAWQNILEKKEGPS
                     VLILTRQKLPLLSKYQNISDISRGGYIVYQGGKDPYVVISASGSEVETALQVKDILEG
                     EHISCRVVSIPDRETFSRQDFKYKESILNLKKSLQVVIEAATGQGW"
BASE COUNT          604 a          485 c          332 g          670 t
ORIGIN      
        1 ccacccctgc ccggtagcgg cttcgattac tacctgtaga gattttttta aatttaaaat
       61 actctcttta tacttaaaat cctgtctgga aaatgtctca cgatctggaa tagatactac
      121 ccgacaggaa atatgttctc cttccaaaat atccttcacc tgaagtgcag tctctacctc
      181 gcttccactg gctgagataa ccacatacgg atctttacca ccctggtaaa ctatgtaacc
      241 tccacgtgaa atatcgctga tattctgata tttgctaagt aagggcagct tctgcctggt
      301 taatattaaa acagagggac cttccttctt ttctaaaata ttttgccagg ctactttaac
      361 ttcatcatta tcggcaggac gaattacctg caaattaggt ataatcctta atgattcaag
      421 ctgttcaatg ggttgatgag ttggaccatc ttcaccaaca tacactgaat catgagtaaa
      481 aataaagatt accggtaaat tcatcagagc agccagcctg attgccggcc tcatataatc
      541 tgaaaatact aaaaaggtag agcaataggg ccttattccc ttatgaagag ctaaaccatt
      601 acagatagca cccatagcat gctccctcac tccaaaacgg atattacgag cattataaca
      661 atttttttgc agctcagaga atttttccag ataggtcatg gtagatggtt ctaaatctgc
      721 tgaaccacct ataatatatg gaatttcttc tgcaatctta tttaaaattt ctccagaaga
      781 acttctggta gcaatttttt cggctaaatc cattttccag aatcccattt cttccggtac
      841 ctttaaatca atcgactcat ccaattgcct tcttttttcg ggaaattttt ctgaccagga
      901 aataaattta tcatcccatt gccttcttaa ctgccttaat tctgattttc ttttttcgaa
      961 aaattctctt acctcattgc taacatagaa agttttatct gctggcaaac caagattttt
     1021 tttcaacccg attacctctt catggccgag tggtgctcca tgtgaggtat gactatcctg
     1081 tttggtaggc gcaccaaagc caatatgggt ctgaacaatt ataagaaccg gtttatcact
     1141 cttttctcgg gctttcatca gtgctccctt tagctcatcg aaatcatggc cattaacatt
     1201 accgattacc ctccagttat aagcacggta tctttctgcc actgattcag taaaagttaa
     1261 atcagtcctg ccagccagag aaatctgatt ttgatcatat ataacaatca atttttccaa
     1321 tccccaatga ccggctaaag aggcagcctc tgaggtaatc ccttccatca tgcagccatc
     1381 actggctaaa acataggtat aatgattaac aatatcaaaa tctttttcat taaatttttc
     1441 agccagtatc ttttcagcga gagccatgcc aaccccgttg gcaaatccct gtcccaatgg
     1501 accggtagtg acttcaactc caggggtatg cctgtattca ggatgtccgg gagtctttga
     1561 accaatttgt ctgaaattca ttatatcttc tatagtgata tcataaccag atagatgtaa
     1621 taaggcatag agcagcattg agccatgacc ggcagatagg ataaatctat ccctatcaag
     1681 ccaatctggc ttttcaggat catgtttgag tatctcagca aatagaactg ctcccaggtc
     1741 agcacatccc agaggcaacc ccgggtgtcc cgagttagct ttttctattg catcaacaga
     1801 taagcctctt attatttttg caacctcttc taacatctct cctccaatcc atattcatat
     1861 aaatactata ttcgttatag tttttacttt cttttctata aatctaatat cattacaacc
     1921 aatgcaatat ggtaaaaata tatttattat caccttttta atactcgtca tgttcggttc
     1981 tgctgataat ctcttcctgc agagtcttgc tgaggttatt gaaataatca ctataaccgg
     2041 ctacccgtac aataagattc tgatactcct ctggattttc ctgagcagcc c
//
LOCUS       DAOQWT010000297         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_61531_length_2088_cov_2.572061, whole genome shotgun sequence.
ACCESSION   DAOQWT010000297 DAOQWT010000000
VERSION     DAOQWT010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61531_length_2088_cov_2.572061"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..>2088)
                     /locus_tag="PKJ95_06075"
     CDS             complement(4..>2088)
                     /locus_tag="PKJ95_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65839.1"
                     /translation="EIYRDYSQDLTNSQENRIINYDDRDIDTPLPHIMIWPPLHPENW
                     KDSYYAFIYGKRYNDGVNQEEIVPLEFKDQDFKDINYERSRKADKIEIFRLETLPTYI
                     MISDIASNGKGLLILNHKSLTTVMNPEKSAKVGIDFGTSHTNIAITIDDSEPEVLKYS
                     SGFSGKNLNDNDFITLIRFSEPELGREQIPNLIYNYLKQYFLPNSLSEIHNNQSVDMP
                     LPSMILMEGDTDSYKALLNTSIAFSKDDVGLFNYDLDGMTIKKTYVQQTNLKWEPQLI
                     KQQASKEYLRILLLLLKYELIKRGVNLKKVEYHWAFPKSFSETLINDYKKMWRELVGE
                     GYKITDESKAALLYFDHKGILPRNTPDIAIIIDTGGGSSDISIWQDGQILMLYSSLWA
                     GMNLVGFEKDNEIYSIIYDAIINLSSTKRDIFQNIKGFQNQLNYILYSLDETTLSNLA
                     RSDSFYKIRFIILYFYSALLYEIGIQSRAIEMDNIKKINLYLAGNGSRFACWSSGAVD
                     KIDEQEQEIYKQVIKKAISFDKEVEIHTSSAQDKKREVAIGLCRGNEELRAKEASKKQ
                     LIAEKVFIEANSDLSNMTIDEFNELILQNGHNIHLDQNKSEIVNFHNTFFEVLAKSNL
                     YRDRLKNNSALSNLERIKNILIGDGGKFIGELRGVIEDNIKNYNSISSSVFILGMQIT
                     INRLHRYLSKNQ"
BASE COUNT          676 a          352 c          284 g          776 t
ORIGIN      
        1 attctattga ttttttgaaa gataacgatg taatctatta attgtaatct gcattccaag
       61 aataaatact gagctggaaa tagaattata atttttaata ttatcttcta taactcctct
      121 caattcacca atgaacttac ccccatcgcc aattaatata ttttttattc tttcaagatt
      181 agataaagca ctattatttt tcaatctatc cctataaaga ttactttttg cgagtacctc
      241 aaagaaggtg ttatgaaaat tgactatttc agatttattt tggtccagat gtatattatg
      301 tccattctgt aaaattaatt cattaaattc atctattgtc atattagaga ggtcagagtt
      361 cgcttcaata aatacttttt ccgcaattaa ttgtttttta cttgcttctt ttgctctgag
      421 ttcttcatta cctctacaaa gtcctatggc tacttctctt ttcttatcct gagcagaaga
      481 agtgtgaatt tctacctctt tatcaaaaga gatagctttt ttaattactt gtttataaat
      541 ctcttgttcc tgttcatcta ttttatccac tgctccactg ctccagcagg caaatcgtga
      601 accattacca gccaggtata ggttaatttt tttgatatta tccatctcaa ttgctctgct
      661 ctggattcca atttcataca gtagagcact atagaaatag agaattataa agcgaatttt
      721 atagaaagaa tctgaccttg ccagattact caatgtagtt tcatctaatg aataaagaat
      781 ataattcaac tgattttgaa accctttaat attttgaaag atatctcttt tagtagaaga
      841 taaattaatg atggcgtcat aaattattga atatatttca ttatcttttt caaatccaac
      901 cagattcatc ccagcccaga gagaactgta aagcataagg atttgaccat cttgccagat
      961 agaaatatca gatgaaccgc ctcctgtatc aataattatt gcgatatctg gagtgttacg
     1021 aggaagaatt cctttatggt caaaatatag taaagcagct ttactttcat ccgtaatttt
     1081 atatccttct cctactaatt cacgccacat ttttttataa tcattgatta aagtttcaga
     1141 aaaagattta ggaaaagccc agtgatattc tacttttttt aaattgactc ctcttttaat
     1201 taattcatac tttaacaaga gcagtaatat cctgagatat tcttttgatg cttgttgttt
     1261 tattaattga ggttcccatt ttaaattggt ttgttgcaca taagtttttt taattgtcat
     1321 tccatctaaa tcataattga acaatcctac atcatcctta gagaaagcaa tagaggtatt
     1381 taataatgct ttataagaat cagtatcacc ttccattaat atcatagaag gaagaggcat
     1441 atcaacagat tgattattat gaatctcact tagtgaattg ggtaagaaat attgttttaa
     1501 ataattataa atcagattag gaatttgttc tcttcctaat tctggttcag aaaatcttat
     1561 gagagtgata aaatcattat cattcagatt tttacctgaa aatccagaac tatatttcag
     1621 cacttctggc tcagaatcat caatcgtaat agcaatatta gtatgggaag ttccaaaatc
     1681 aataccaact tttgcagatt tttccggatt cattactgta gtcagggatt tatgattgag
     1741 aattaataaa cccttcccat tagaagcaat atcagaaatc ataatataag taggaagtgt
     1801 ttctaatcgg aatatttcta ttttatcagc tttgcgagat ctctcataat ttatatcctt
     1861 aaaatcctgg tctttaaact ccagaggcac tatttcttcc tgatttacac catcattgta
     1921 tcttttccca taaataaaag cataatagct atctttccag ttttcaggat gtaggggagg
     1981 ccaaatcatt atatgtggaa gaggtgtatc aatatcccta tcatcataat ttataatacg
     2041 attttcctgg ctattagtta aatcctgaga ataatcccgg taaatttc
//
LOCUS       DAOQWT010000298         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_61753_length_2083_cov_3.036489, whole genome shotgun sequence.
ACCESSION   DAOQWT010000298 DAOQWT010000000
VERSION     DAOQWT010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61753_length_2083_cov_3.036489"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1136)
                     /locus_tag="PKJ95_06080"
     CDS             complement(90..1136)
                     /locus_tag="PKJ95_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNR65840.1"
                     /translation="MDRVIVYSAINTKIRILERGFLSRQDYSNMLQMTSVSQVASYLK
                     ENTSYRDILASIQTDKVSRRDIEDTLTTNMIINMDKLIYYFRGDYKVLIRSLYLKYEI
                     EDLKILARSIFNGKKAEEIESSLSFLGKYSKIEPERLFQAETIRDLIYSLKGSQFFEF
                     LIPLVDGRRENLFRFEMALDTGYFNIIQSRKLNILSLDEYLIKKWEGLLADLYNIQWI
                     YRGKKFYQLSPEELLNYTINFGDRLHYKERQRLCYSKSPADLHQMTSGLVYGFLFKKE
                     EISTDIYMERRINRFLFYKLKILNKKFPLTIIQTITYIWRLEMEIRDIISIVESIRYN
                     IPAEEIKKYLVRAA"
     gene            complement(1136..1471)
                     /locus_tag="PKJ95_06085"
     CDS             complement(1136..1471)
                     /locus_tag="PKJ95_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65841.1"
                     /translation="MEDKNIIENLINLDSQASEINKKRKTQLQDLSQKYREDEQNIIK
                     NYKKQIDQDTHSALEKIEMETKQEISQIRLKNKNLLENMQQKFEQNLTNIVDEIMERI
                     FELKKKEDG"
     gene            complement(1609..>2083)
                     /locus_tag="PKJ95_06090"
     CDS             complement(1609..>2083)
                     /locus_tag="PKJ95_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNR65842.1"
                     /translation="IIQHKTRQKTLAFGITAVEGIAIHSFIDGLIYTVTFSSSIFTGF
                     LAGMGLVVHEFAEGVITFSVLIEGGFKSRRAFWFAFLMSGLTTPLGALIAYPIISKLN
                     PAVLGLSLGFVVGVLIYLSASHLLPEARGHEKKHSILAFIAGIVLALFIIMTEKA"
BASE COUNT          620 a          419 c          307 g          737 t
ORIGIN      
        1 atactccgtt taaaatattc tttggcccaa taatccccat cactttcatt tcttcaacac
       61 tcatagttaa gacctctttg ttttaaacct tatgctgccc ttaccaaata ttttttaatc
      121 tcttcagcgg gtatgttata ccttatgctc tctacaatag agataatatc tcttatctcc
      181 atctctaatc tccaaatata ggtaattgtc tgaataatag tgagtggaaa tttcttgttt
      241 aaaattttga gcttataaaa aagaaacctg tttatgcgtc tttccatata aatatctgtc
      301 gatatctctt cttttttaaa taaaaaacca tagaccaatc ctgatgtcat ctgatgcaag
      361 tctgccgggc tctttgaata acacaatctc tgacgttcct tatagtgcaa tctgtcacca
      421 aagtttatag tataattcaa caattcttcc ggggataact gataaaactt ctttccccgg
      481 tatatccatt gaatattata caaatctgcc agcaagccct cccatttttt gattaaatac
      541 tcatctaaag acaggatatt caatttacgg ctttggataa tgttgaaata accggtatct
      601 aaagccatct caaagcgaaa tagattttcc ctcctgccat caaccagcgg tattaaaaac
      661 tcaaaaaact gggaaccttt caatgaatat attaaatctc tgatggtctc tgcttgaaaa
      721 agcctttcag gctcaatctt gctgtatttg cctaagaaag atagtgaact ttctatttct
      781 tcagctttct taccattgaa aatacttctg gctaagattt ttaaatcttc tatctcatat
      841 ttaaggtaaa gggaacgaat cagaacctta taatcaccac gaaagtaata aattaatttg
      901 tccatattaa taatcatgtt tgttgttaga gtatcttcaa tatctcgcct gctgacttta
      961 tcggtctgta tgctagccaa gatgtcccgg taagaggtat tttcttttaa ataactggcc
     1021 acctgagaaa cagaggtcat ttggagcata tttgaataat cctgcctgct taaaaaccct
     1081 ctttccaaaa ttcttatttt ggtattaatc gctgaataga ctataaccct atccattatc
     1141 catcttcttt ctttttaagc tcaaagattc gttccattat ctcatcaaca atatttgtta
     1201 aattttgctc aaacttttgc tgcatattct ctaataaatt cttatttttt aatcttattt
     1261 gactgatttc ctgttttgtt tccatttcta tcttctcaag ggcagagtga gtatcctggt
     1321 ctatttgttt tttataattt tttataatat tttgttcatc ttctctatat ttctgggata
     1381 aatcctgtaa ttgtgttttt ctctttttgt ttatctcaga tgcctgagaa tctagattaa
     1441 taagattttc tatgatattt ttatcttcca aaatagcact ctcccttctc ttctaacggt
     1501 cggttaatcc tctcacaaac gaagatagta atagcttact attgcttaaa tctgattata
     1561 actctatagg tcaatatatg taagcattat tctttagacc ttaggccctc aggccttttc
     1621 ggtcataata ataaataggg ccaggacgat accagctata aaagctaaga tggaatgttt
     1681 tttctcatga cctctggcct ccggcaggag atgggaagcg gagaggtaga ttaacactcc
     1741 taccacaaaa ccgagtgaca ggcctaagac tgctgggttt aatttagata taatggggta
     1801 ggcaattaaa gctcctaagg gtgtggtcaa tcctgacatt aaaaaagcaa accagaatgc
     1861 cctcctgctt ttaaaacccc cttcaattaa gacagaaaaa gtgataacac cttccgcaaa
     1921 ttcatgtact accagtccca ttcctgctaa aaaaccggta aatatactgc tgctgaaggt
     1981 taccgtatat attagtccat ctataaagga atgtatggct atcccttcca ccgctgtgat
     2041 accaaaggcc agggtttttt gtctggtctt atgttgaata att
//
LOCUS       DAOQWT010000299         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_61788_length_2082_cov_4.088801, whole genome shotgun sequence.
ACCESSION   DAOQWT010000299 DAOQWT010000000
VERSION     DAOQWT010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61788_length_2082_cov_4.088801"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..965)
                     /locus_tag="PKJ95_06095"
     CDS             complement(96..965)
                     /locus_tag="PKJ95_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNR65843.1"
                     /translation="MKEIVIVSGKGGTGKTVIAGSFAVLAKNKVMGDCDVDASNLYLL
                     LHPQVKEKHDFYAGVIPEIDLTKCNQCGLCEELCRYDAITVSQDGAFVDYISCEGCGL
                     CGLACPEKAVEMKENLCGEWYISETQYGPLVYARLGIAEENSGILVTTVRKNARVIAE
                     NRNLDLIIIDGPPGIGCPLIASITGANLVVVVTEPTLSGIHDLERVIEVSQHFNVEPV
                     VIINKFDINLENTSSIEEFCKENSISVIGRIHFDNIITEALVKGLPVVEHSDNKVTQE
                     IKDIWQEIFKKIQ"
     gene            complement(983..1891)
                     /locus_tag="PKJ95_06100"
     CDS             complement(983..1891)
                     /locus_tag="PKJ95_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR65844.1"
                     /translation="MIISVASGKGGTGKTTIAVNLALALAGDKKNNVYFLDCDVEEPN
                     AHLFLKPLIKKSEPVEIPIPKIDYNKCNFCGKCARACLFNAIAVAKNKVLIFPELCHG
                     CGACTYICPEGAISEKGREIGIIEEGEAGLSNFMEGRLNIGEAIAPPIIKRIKKRGIE
                     KYQSEVNKNKNGSKQHILLIDAPPGTSCPVIESIKESDYTILVTEPTPFGLHDLVLAV
                     EVLKKLQIPYGVVLNKCDIGDHQVEKYCQEKEIPILMSLPMDKEIAIAYSKGIPLVNL
                     GYSYKQKFISLFQNIVTEYKLAKVRN"
BASE COUNT          597 a          480 c          315 g          690 t
ORIGIN      
        1 tattattgat tcctaaacta cgtcctaaac gacgggaagg gacaggtcca aaagtaagca
       61 tggttactcc ttcatttcat atataaaatg aggaactatt gaattttttt aaatatttct
      121 tgccagatat cttttatttc ttgagtcact ttattatcac tatgctcaac tactggcaat
      181 ccttttacta aggcttctgt tattatatta tcaaaatgaa ttctaccaat tacagaaata
      241 ctattttctt tgcaaaattc ttctatacta gaagtatttt ctaaattaat atcaaattta
      301 ttaattatta ctaccggttc aacattgaaa tgctggctaa cttcaattac tctttccagg
      361 tcatgtatcc cggagagcgt aggttcggtt actaccacca ccaggtttgc acctgtgata
      421 gaagctatca ggggacagcc gattcccggt ggtccgtcaa tgatgattag gtctaaattc
      481 ctgttttcag caattactct ggcattttta cggactgtgg ttactaatat tcctgaattt
      541 tcttctgcaa tacccaatct ggcatagact aagggtccgt attgggtttc agaaatatac
      601 cattcgccac aaagattttc cttcatctct acagccttct cgggacaggc cagtccacat
      661 aatccgcaac cttcgcagga aatatagtcg acaaaagccc cgtcctgaga tactgtaata
      721 gcatcataac ggcataactc ttcgcataat ccacattgat tacattttgt taaatcaatt
      781 tctggaatta ccccggcgta gaaatcatgc ttttctttta cttggggatg tagcaaaagg
      841 tatagattag aggcatcaac atcacaatct cccattactt tattttttgc taaaactgca
      901 aaacttcctg ctattaccgt cttacctgta ccacctttgc cactgacaat gactatttct
      961 ttcatcaggc aaacctcaat tcctaatttc tcacttttgc aagtttatat tctgtaacga
     1021 tattctgaaa aagtgaaatg aatttttgtt tataagaata acccaggttt actaaaggta
     1081 tacctttaga ataagctata gctatctctt tatccatagg cagagacatt aatattggta
     1141 tctccttctc ctgacaatac ttttctacct gatggtctcc tatatcacac ttatttaaaa
     1201 ccactccata tggaatctgt aattttttta aaacctctac tgctaaaacc aggtcatgca
     1261 agccaaaagg agtcggctcg gttaccagta ttgtataatc gctttctttt atcgattcga
     1321 taaccggaca ggaagtacct ggaggtgcat caatcagaag aatatgctgc ttactcccgt
     1381 ttttgttctt atttacctcc gattgatatt tttctatacc cctcttttta attctcttaa
     1441 ttatgggcgg agctattgcc tctccaatat ttaaacgacc ttccataaaa ttacttaaac
     1501 ctgcctcccc ttcctctata atcccaatct ctctgccttt ctcgctgatt gccccttccg
     1561 gacaaatata agtacaggct ccacagccat ggcacaattc cgggaagatt agaactttat
     1621 ttttagcaac ggctatggca ttaaaaaggc aagctctggc acatttccca caaaaattac
     1681 atttattata atcaatttta ggaattggaa tttcaactgg ctcacttttt ttaatcaggg
     1741 gttttaaaaa aagatgggca ttgggctcct caacatcaca atccaaaaaa tagacattat
     1801 ttttcttatc tccagctaat gccaaagcaa gattcactgc aatggtggtc ttaccagtac
     1861 cgcctttacc actggcaact gaaataatca tatatccaca atccttttta acttcttact
     1921 gattactgtc ttatcatcat tgttccacat ttagggcatt tcataccggt gcagggtatt
     1981 cctgtctgat gaggaactat agcgccgcag gaaggacaaa cacagttacc gtctaagcct
     2041 agccccattc ctccgcttcc ccttcccacc tgcctgccac cc
//
LOCUS       DAOQWT010000300         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_61849_length_2081_cov_2.838598, whole genome shotgun sequence.
ACCESSION   DAOQWT010000300 DAOQWT010000000
VERSION     DAOQWT010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61849_length_2081_cov_2.838598"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..2041)
                     /locus_tag="PKJ95_06105"
     CDS             complement(26..2041)
                     /locus_tag="PKJ95_06105"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HNR65845.1"
                     /translation="MEQNMNREHHNNKYNHHEPTKHNHTNHHQHMAEDFRKRFIISTF
                     ITIFILLLSPLIQQFFHYRLDIRGGKYILFVLSSFIFFYGGNPFLKGFIDELNKKQPG
                     MMTLITLATSVAYLYSSAVVFGLEGEIFFWELATLIDVMLLGHWLEMKSIIGASKALE
                     KLAQLMPDIAHRIKGKEVLVVKISELERGDIILVKAGEKIPADGIIVRGNSYIDESML
                     TGESKPVLRKEKDRVIGGSINGDSILEVKVEGVGEDSYLSKIINLVREAQTSKSKTQV
                     LADKAATWLTVISLTIGALTFIFWLTGGSKLAFAIERMATVMVITCPHALGLAIPLVV
                     AVSTSLSAKNGLLIRNRSAFENSRKITTVVFDKTGTLTEGRFGVSNVTLIDKEYDEKQ
                     LIQLAASLEAYSEHPLAKGIMKRAEELKANIIPVSDFEVIKGQGVKGKIEGKSIALVS
                     QNYVKKNNPEILKQFNTNEAGTLVYILLDGKIIGLITLSDKIREESYKAIERLQKMGI
                     KCWMLTGDNQNIAREVSDKLNLDGYMAEVLPHEKLEKIRNLQDKGEFVAMTGDGINDA
                     PALAQADVGIAIGSGTDIAAETADIILVKSNPSDVFSLILFGRATYRKMLQNLFWATA
                     YNAIAIPLAAGVLYNSGIVISPAIGAALMSLSTIIVAINAQFLKVEK"
BASE COUNT          622 a          434 c          327 g          698 t
ORIGIN      
        1 aggaaactat aattaaaaat taatattact tttctacttt taagaattgt gcattaatcg
       61 ctacaataat tgtgcttagt gacattaaag ctgcaccaat ggcgggagaa atgactatac
      121 ctgagttata cagcactccg gcagccagag gaatggcaat ggcattataa gcagtcgccc
      181 agaatagatt ctgaagcatt ttacggtatg ttgcccttcc aaataatatc agtgaaaata
      241 catctgaagg attgcttttt accaggataa tatcagcggt ctcagctgca atatcagtac
      301 ctgagccaat agcaatccct acatctgctt gagcaagagc tggggcatca tttattccat
      361 caccggtcat ggccacaaac tcacccttat cctgaagatt tcttattttt tcaagcttct
      421 catgtggcaa cacttctgcc atataaccat caagatttaa tttatcagaa acttcccgtg
      481 ctatgttttg attatcacct gttaacatcc agcattttat acccattttt tgcagccttt
      541 ctatcgcctt ataagattcc tctctaatct tatcagatag agtaatcagc ccaattattt
      601 tcccgtccaa taaaatataa actaaagttc ctgcctcatt ggtattaaat tgttttaata
      661 tctcaggatt atttttcttt acatagttct ggctgaccag ggcaatagat tttccttcta
      721 ttttaccttt taccccctgt ccttttatta cctcaaaatc agaaacaggt ataatatttg
      781 cttttaattc ctcagctctt ttcataattc ctttagcaag aggatgctcg gagtatgctt
      841 ccagagaggc agccagctga atcagttgct tttcatcata ttctttatct ataagagtaa
      901 cattgcttac cccaaacctt ccttcagtca atgtaccggt cttatcaaat acaactgtag
      961 taatctttct agaattttca aaggcactcc tatttcttat caaaagacca tttttggccg
     1021 ataaggaagt cgagacagca acaactaaag gaatggctaa gcccaaggcg tgaggacagg
     1081 taattaccat aacggtggcc attctttcta tagcaaatgc cagttttgag ccaccggtaa
     1141 gccagaaaat gaaggtcaat gctcctatgg tcaaggagat tactgtgagc caggtcgctg
     1201 ctttatcggc aagcacctgt gtcttcgatt tggaagtctg tgcctctctt accagattaa
     1261 taatttttga caggtaagaa tcttctccaa ccccttccac tttcacctct aaaatcgaat
     1321 ctccattaat ggaacctccg ataactctat ctttttcttt tctgagtacc ggctttgatt
     1381 caccggtcag catagattca tcaatatagc tatttcctct aacaatgatt ccatcggcag
     1441 gtatcttttc tcctgcttta actaagataa tatctcctct ttctaattct gatatcttga
     1501 ccactaaaac ttcttttcct ttaattctat gagctatatc tggcattaat tgagctaatt
     1561 tttccaatgc ctttgaggca cctattattg atttcatctc cagccaatga cccaatagca
     1621 taacatcaat aagggtagcc agctcccaga aaaagatttc tccctccagt ccaaaaacaa
     1681 cagcactgct atataaatag gctacagagg tagctaaagt aattagggtc atcatcccag
     1741 gttgtttttt atttaattca tcaataaatc ctttcagaaa aggattccca ccatagaaaa
     1801 atataaaact ggagagtaca aagagaatat attttcctcc ccgaatatct aatctatagt
     1861 gaaaaaactg ttgtattagg ggagataata atagaatgaa aatagttata aaagttgata
     1921 tgataaatct ttttctaaaa tcttctgcca tatgctgatg atgattggta tggttgtgtt
     1981 ttgtgggttc atgatgatta tatttattat tatgatgttc gcgattcata ttttgttcca
     2041 ttattaatcc tctttattac ttaaaaagga gtattttatt t
//
LOCUS       DAOQWT010000301         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_62061_length_2077_cov_2.261622, whole genome shotgun sequence.
ACCESSION   DAOQWT010000301 DAOQWT010000000
VERSION     DAOQWT010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62061_length_2077_cov_2.261622"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PKJ95_06110"
     CDS             <1..851
                     /locus_tag="PKJ95_06110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR65846.1"
                     /translation="EAHYGLGLTFLKLEQFFLAIDSFQQALKLKPNDFQSLYGLGIIY
                     KQIGEEQKAIQVLNQALGIKPDYGEAYYELGMLYQGLEQNQDAVNALERAAHYLPKQA
                     DRIFFQIGMIYKKIGSFDKAIEALERAVKNNEEFVDAYYYLGWFYNWNRQFDKAIEAY
                     EKVISLNPDYPDVYYNIGWAYGEMGEYLKAVETFQRAIEKTPDDVDSYYALGWVYGKL
                     EDYEEAKKAFQETIRLEPDYIYAHYGLGVTFLALGERSMALEEYKILKGLDQDIADLL
                     FEQIYP"
     gene            893..1951
                     /locus_tag="PKJ95_06115"
     CDS             893..1951
                     /locus_tag="PKJ95_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNR65847.1"
                     /translation="MLGNIFQQVYFQNRIIDYLMALLIFIIASLTIRFLKNFSIKILE
                     GLAGKKGTKIDKRFVDAFQDRIKPFINLLYFAAFYFSFYQLKIPIIIESYFNIIIIAL
                     FIFYTVKSIISIFSYFLENYWIKKEKDGTRITALRGIETFLKIMVWSIAFIVFLDNLG
                     VQISALLAGLGIGGIAIALASQNILGDLFSYFIIFFDRPFEIGDFLSIDNFSGTIESI
                     GIKTTRIRSLGGEELIFSNTNLVNSRLRNYKRMKKRRVSFNFGVTQQTSLEQLKEIPD
                     ILNNILQEISGASLDRAHFSAFGDYGLIFEVVYYVNNRDYNQYMDIQQEINLRLKEEL
                     EKRSIKFAYPTQTVLLSQ"
BASE COUNT          685 a          299 c          394 g          699 t
ORIGIN      
        1 cagaagcaca ttacggttta ggattgactt ttttaaagtt agaacagttt tttctggcta
       61 ttgattcctt ccaacaggct ttgaaattaa aaccaaatga ttttcaatca ctttatggat
      121 taggaattat ctataaacaa attggtgaag aacaaaaagc tatccaggtg ctgaatcagg
      181 ctctgggaat aaaaccagat tatggagagg cttattatga attaggaatg ctctatcaag
      241 gtttggagca aaatcaggat gctgttaatg cactggaaag ggctgctcat taccttccta
      301 agcaggctga tcgaattttc tttcaaatag gaatgattta caaaaagata gggagttttg
      361 ataaagcaat agaggctctg gaaagagccg taaagaataa tgaagagttt gttgatgctt
      421 attattacct gggctggttt tacaattgga acagacagtt tgataaagca atagaggctt
      481 atgaaaaagt tatctcttta aaccccgatt atcctgatgt ctattacaat ataggctggg
      541 cttatggtga aatgggagaa tacttaaaag cggtagaaac atttcaaagg gcaatagaaa
      601 aaactccaga tgatgttgat tcttactatg ctctgggttg ggtatatggt aaattagaag
      661 attatgaaga agcaaaaaaa gcctttcagg aaacaatacg tcttgagcca gactatattt
      721 atgcgcatta tggtctgggc gtgacttttc tggcattagg tgaaagaagt atggctttag
      781 aagaatataa gatattgaag ggtttagatc aggatattgc tgatttgcta tttgaacaga
      841 tttatcctta agattattat tttttcaaaa agatatttgt gggagtaaga taatgttggg
      901 caatatattt cagcaggttt attttcaaaa ccggattatt gattatctga tggctctcct
      961 catttttata atagcctcat tgactatcag attcttaaaa aatttttcaa taaaaatatt
     1021 ggaggggctg gcaggcaaaa aaggtactaa aattgataag cgttttgttg atgcctttca
     1081 ggataggatt aagccattta tcaacctcct ttattttgcc gccttttact ttagttttta
     1141 tcaactgaag attcccatca ttattgaaag ttactttaat atcataatca tcgctttatt
     1201 tatattttat actgttaaat ccatcatatc gattttctct tattttttag agaattattg
     1261 gattaaaaaa gaaaaagatg gtactcgaat cactgcccta agagggattg aaacattcct
     1321 caaaattatg gtatggtcta tagcttttat tgtcttttta gataacttag gggttcagat
     1381 atctgctctt ctggcaggat taggcattgg tggtattgct atagctctcg cttctcaaaa
     1441 tattctgggt gacttattca gttattttat aatcttcttt gaccgaccct tcgaaattgg
     1501 cgatttttta agtattgata atttttcggg aacaatcgag agtatcggga taaaaactac
     1561 ccggatcagg agtctgggag gagaagaact catcttttcc aataccaatc tggttaattc
     1621 acgtttgcgt aattataaac gtatgaaaaa aagaagggtg tcctttaatt ttggagtaac
     1681 acaacaaacc tctttggaac aattaaaaga aatacctgat atattaaata atatactcca
     1741 ggaaataagc ggagcatcct tagatagggc tcatttttct gccttcggag attatggtct
     1801 tatttttgaa gtagtttatt atgtaaataa ccgtgattat aatcaatata tggatatcca
     1861 acaagaaatc aatttaagat taaaagaaga attagaaaaa aggtctataa aatttgccta
     1921 tcctactcaa actgttcttc tgtcccaata aggcaaaaaa tactcccggg gaacagataa
     1981 tattatcaat cttaaaatat taaatatgtt tttaaaaaag aaaggtggat cctgtgcaga
     2041 tttttctatt tgttgccgct ttaattgcca ttttagc
//
LOCUS       DAOQWT010000302         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_62461_length_2068_cov_3.442126, whole genome shotgun sequence.
ACCESSION   DAOQWT010000302 DAOQWT010000000
VERSION     DAOQWT010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62461_length_2068_cov_3.442126"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKJ95_06120"
     CDS             complement(<1..214)
                     /locus_tag="PKJ95_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNR65848.1"
                     /translation="MNSKETERAEPVNFIQAIIDKDKENNKYGGRVHLRFPPEPNGYL
                     HIGHAKSICLNFGLAEKNNGLCNLRFD"
     gene            377..535
                     /locus_tag="PKJ95_06125"
     CDS             377..535
                     /locus_tag="PKJ95_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65849.1"
                     /translation="MSLEEFKNRATITGSVDISRAPGSVSVPLLIPIEVLEKVGLYDI
                     IKEHYNLK"
     gene            complement(742..948)
                     /locus_tag="PKJ95_06130"
     CDS             complement(742..948)
                     /locus_tag="PKJ95_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNR65850.1"
                     /translation="MDSVYKIIEIVGTSESSWEDAAKTALDTAAKTIDDIRIAEVSKL
                     DIKMSTDGNIFFRTKLKVSFKYRS"
     gene            complement(1009..>2068)
                     /locus_tag="PKJ95_06135"
     CDS             complement(1009..>2068)
                     /locus_tag="PKJ95_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-triphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNR65851.1"
                     /translation="SKPTREKLSWHVKQMRYSGEPGFMNAEAAARRRPNLEGANPCME
                     VLLDREQMCNLTTVNVMGFVRDEKLDEKKLFEAQRLSVRAAFRMTFLDLELDEWDIKH
                     KRDRLIGCSLTGWQDMVNAISISSEGESHLRKKLQEVAHQEADSYAKKLGVNPPLLVT
                     TVKPEGTVTQLPTVSSGIHYSHAPYYIRRVRINASDPLLKVCEELGYDIKPENGQTEE
                     NCRTKVISFPCSAPPGKTKNDVSAIEQLENYKAFQREYSDHNTSITVHVRNNEWEEVE
                     EWLWNNWDDVVAVSFVSLDDSFYAQMPYEEISKEEYEKRVKDMVHFIPSLISKYEKEE
                     ITLDPGDESCDNGVCPVR"
BASE COUNT          581 a          431 c          352 g          704 t
ORIGIN      
        1 catcaaaacg aagattacat agtccattat ttttttctgc cagtccaaaa tttaaacaga
       61 tggacttggc atgtccgata tgtaaatatc cattgggttc cggtgggaaa cgtagatgta
      121 cccttccacc atacttgttg ttttccttgt ccttatcgat gattgcctga ataaagttta
      181 ctggttctgc tctttctgtt tccttagaat tcatttaata cctttcctcc tgctttctcc
      241 ttttcaattc tttcattttt ccttttcgtt tgaataaaat caaaattggt tcaattgaaa
      301 aatgaatgtt tatgatgtag tgtctttata ttacaacatc taaaacaaaa aatctataag
      361 ttcaaaatgt ttggaaatgt ctttagagga atttaaaaac agagctacta taaccggttc
      421 tgtagatatc agtagggcgc cgggttcagt ttctgtgcca ttactgatac cgatagaggt
      481 cctggaaaaa gtcggcttat acgacattat caaagaacac tataacctta aataaggtta
      541 gaaaacattg ctgaggagta aaaatttccc atagacaaaa taataatccc cacacgtaac
      601 gggtgaggat tattattttg ttttaatagc atagaaactg agaaatttga gttaaaatct
      661 attacatttt cggtggtata attgtaatta tctaacaatt tcaaggtttt aggcttgaaa
      721 cagctacaaa attattatag gttagcttct gtatttaaag gatactttta attttgttct
      781 aaaaaatata tttccgtctg tgctcatctt tatatcgagc ttactgactt ctgcaattct
      841 gatatcatct atggtttttg cagcagtatc cagtgccgtt tttgccgcat cttcccatga
      901 actttcactg gttcccacaa tctcaattat tttataaaca ctatccatat ttgacctcct
      961 ttaattttat tttaaaaact ccttcttgat aatacaagtt ccatttgctt atctaacggg
     1021 acaaacacca ttgtcacagg attcatcacc aggatcaaga gttatttctt ctttttcata
     1081 ttttgaaata agagaaggaa taaaatgaac catatcttta actctctttt catattcctc
     1141 tttacttatc tcttcatagg gcatctgggc ataaaaactg tcatctaagg agacaaaact
     1201 gaccgccacc acatcatccc agttattcca caaccactct tccacttcct cccattcatt
     1261 atttctaaca tgtacagtaa tggaggtgtt gtggtctgaa tactctctct gaaatgcctt
     1321 ataattttct aattgctcaa tagcagatac atcatttttt gtttttcctg gcggtgcact
     1381 acagggaaaa gatattacct tggtgcggca attttcctca gtctgaccat tttccggttt
     1441 gatatcataa ccgagctctt cacaaacttt aagcagggga tctgaagcat taatacgcac
     1501 tcttctaata taatagggtg catgggagta atgaatgccg cttgaaactg taggcagctg
     1561 ggtgacagtc ccctctggtt taacagtggt gactagaaga ggtgggttta ctcctaattt
     1621 tttagcataa gagtcagctt cctgatgtgc cacctcctgt agttttttac gcaggtgaga
     1681 ttctccttca gaagagatgc tgatagcatt taccatatcc tgccatcctg tcaggctaca
     1741 accaatcagt ctgtctcttt tgtgtttgat atcccattca tctaattcca gatccaggaa
     1801 agtcattcga aaagcggcac gaaccgagag acgctgggct tcaaacagtt ttttctcatc
     1861 taatttctca tccctaacaa accccatcac atttacagtg gtaagattac acatctgttc
     1921 ccggtctaat aagacttcca tacaggggtt ggctccctcc aggttcggtc ttctgcgggc
     1981 tgctgcctct gcgttcataa aaccgggttc cccgctgtac cgcatctgtt tgacatgcca
     2041 ggaaagtttt tcccgagtag gtttggag
//
LOCUS       DAOQWT010000303         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_62504_length_2067_cov_4.061630, whole genome shotgun sequence.
ACCESSION   DAOQWT010000303 DAOQWT010000000
VERSION     DAOQWT010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62504_length_2067_cov_4.061630"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..934
                     /locus_tag="PKJ95_06140"
     CDS             332..934
                     /locus_tag="PKJ95_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNR65852.1"
                     /translation="MQILKEEVKERIKEVALAVFEKYGYQKATMRRIASRADLTVGNL
                     YRYFKNKDDLFDVIIQPAFQEIYRFVSDFDYIKENEFHKKGQKQNFIKAFQEALIKIY
                     GRHRAELLILLNGSKGSHMENAKEEIVHLITDRLKKEIFPKMENDVKPVDDFLAEAFS
                     ISFMEGISLVLGRYEDKETTRELFVKYIHIYFKYFLDELR"
     gene            999..1817
                     /locus_tag="PKJ95_06145"
     CDS             999..1817
                     /locus_tag="PKJ95_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65853.1"
                     /translation="MGRILCKSYILPVRKIITYSFVALLFFLLGIKSSISFTQSDETN
                     LRKGEDSYFQAQELFLESAAQHDVSLQILEESISLFTETEEQPTKYYWLAEVLYLMGV
                     IEKDRNSHEKAKIYFSSSKESISTALKLKDFSEGYRLLADVEGQLIIYGDLLYKTKFG
                     PPIKNMINKAIKLDPGNEKAYFSLAMYYRDAPFIAGGDLKKSEAVLKDMIDINKNTQI
                     DLFSLYLWIETAWVNSEFNPEKISDCISILDLFSNKEDIDSMIARIERKYMNNK"
     gene            1863..>2067
                     /locus_tag="PKJ95_06150"
     CDS             1863..>2067
                     /locus_tag="PKJ95_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HNR65854.1"
                     /translation="MIVVTGAAGHLGNVLIRELLRSGEKIRALILPGEDIISLQGLEI
                     EKVEGNVLDPDSLRKAFRGADTVF"
BASE COUNT          729 a          275 c          382 g          681 t
ORIGIN      
        1 ctgaactaac tcgattgctt gaacagacag agagccgagc ataagaagta aaagtagaaa
       61 agtgcaaaaa acataaggtt tctttttgtt aatcattttt ttcctcctca aaattttttt
      121 ataattatta gacatcagac agaccatttc tacatttgga atattgttat tgggcaattg
      181 atttttttga cttacatatt tattttataa tttattctct gatatttcaa atataactat
      241 tacttattat ttccaaaaaa caggattttt ttaaatattc tcttgacaag tacatttttt
      301 caaatataat aatgaacggg ggtttattat tgtgcagatt ttaaaggaag aggtaaaaga
      361 aagaataaaa gaagtggctt tagctgtgtt tgaaaaatat ggctatcaga aggctactat
      421 gcgtcgcatt gcttccaggg cagatttaac tgttgggaat ctctaccgct attttaaaaa
      481 taaagatgac ctgttcgatg tcataatcca acctgccttt caggagattt atcgtttcgt
      541 aagtgatttt gactatataa aagaaaatga gtttcacaaa aaagggcaaa aacaaaattt
      601 tataaaggca tttcaagagg ccttgattaa aatttatggt agacatcgtg cagaattact
      661 tatcctgtta aatgggagca aaggttccca tatggagaat gccaaggaag aaattgttca
      721 tttgattaca gaccgtttaa aaaaagagat attccccaaa atggaaaatg atgtcaaacc
      781 agtcgatgat tttttggctg aagccttctc tatcagcttc atggaaggta tttcattagt
      841 tttaggcaga tacgaagata aagaaacaac tcgagaacta tttgtaaaat acattcatat
      901 ctattttaaa tattttctgg atgaattaag ataatttttt tttaattaaa taatgaacaa
      961 tcgtttatta tatatttatg atttaaaagg agaattcggt gggcaggata ctttgcaaaa
     1021 gttatatttt accagttaga aagataatta catatagttt tgtggcctta ttattctttt
     1081 tgttgggcat aaagagcagt attagcttta cacaatcaga tgagaccaac cttagaaaag
     1141 gtgaagattc atactttcaa gcccaggaat tatttctgga gtctgctgct caacatgatg
     1201 tttcactaca aattttagaa gaaagtattt ctttgtttac agagacagag gagcaaccaa
     1261 caaaatatta ttggcttgct gaagttttat atctaatggg agtaatagaa aaagatagaa
     1321 atagtcatga aaaagcaaaa atatatttct cttccagcaa agagtcaatc tcgacggctt
     1381 taaaattgaa ggactttagt gaaggctatc gcctgttagc tgatgtagaa ggtcagttga
     1441 ttatttatgg tgacctgctt tataaaacta aatttgggcc accaataaaa aacatgatta
     1501 acaaggctat taaattggac cctggcaatg agaaagctta tttttctttg gccatgtatt
     1561 atcgtgatgc gccttttatc gcaggaggtg atcttaaaaa gagcgaagca gttttaaaag
     1621 atatgattga tattaataaa aatactcaaa ttgatttatt ctcactatat ctttggattg
     1681 aaactgcctg ggttaattct gagtttaatc cagaaaaaat atcagattgt atttcaattt
     1741 tggatttatt ttctaacaaa gaggatatag attctatgat agccaggatt gaaagaaaat
     1801 atatgaacaa taaatgaaat atcttaattt aatcatttat aacaaggggt atataaaaag
     1861 caatgattgt agtaactggt gcagccggac atttaggaaa tgtattgatt cgagaattac
     1921 ttaggagcgg agaaaagata agggcattga ttttgccagg agaggatatc atctctctac
     1981 agggattaga gatagaaaaa gtagaaggga atgttcttga ccctgattct ttgagaaaag
     2041 ccttccgggg agctgatacg gtattcc
//
LOCUS       DAOQWT010000304         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_62607_length_2065_cov_3.457711, whole genome shotgun sequence.
ACCESSION   DAOQWT010000304 DAOQWT010000000
VERSION     DAOQWT010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62607_length_2065_cov_3.457711"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PKJ95_06155"
     CDS             <1..1119
                     /locus_tag="PKJ95_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNR65855.1"
                     /translation="WGCLALGHSHPSVVAAITKQAEKFTHTDFTAVPYASFVQLCEKL
                     SLLAPGNSQKSVALFNCGAEAVENAVKIAKGYTGRSGIVVFENAFHGRTLLAMSMTHK
                     SFPYKYKFGPFASEIYRLPFLSQYHSSIQIDDLERVLINLVNPETVAGVVIEPIQGEG
                     GFNVPKNGFLEKIKELCQKYGMFFIADEIQCGMGRTGKFFAVEHWNIEPDIICLAKAL
                     ASGLPLSAVISRKKITDALPIGTIGGTYVGNPLGCQAALEVIRIIQEEDLLSKAVNIG
                     KIIKDAFTSMKEKYPIIGDVRGLGAMVAMELVKGQKSKEPATEETEKIIQYCVQNGVL
                     IAPAGLNKNVLRVLVSLTISTEQLEEALSVLEEGIAQI"
     gene            1190..1723
                     /gene="pyrR"
                     /locus_tag="PKJ95_06160"
     CDS             1190..1723
                     /gene="pyrR"
                     /locus_tag="PKJ95_06160"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNR65856.1"
                     /translation="MSQVSIIMNKKDIDRTLLRISHEIIEKNKGIEGLGIIGIKTRGE
                     YLAKRIAEYIYGIEKINIPVGVLDITLYRDDLKEKQPQTILVKTEIPFSIEDKKILLV
                     DDVLYTGRTIRAAMDAIVDLGRPKCIQLAVLLDRGHREFPIRADYVGKNVPTSRRELV
                     KVRLGEVDDLNQVEIVK"
BASE COUNT          680 a          330 c          449 g          606 t
ORIGIN      
        1 tggggctgcc ttgctctggg acattctcac ccttctgtcg ttgcggcaat tacgaaacaa
       61 gcagaaaaat ttactcatac cgattttacg gcagttcctt atgcctcctt tgttcagctt
      121 tgtgaaaagt taagtcttct agcaccggga aatagccaaa aaagtgttgc tttatttaat
      181 tgtggagctg aagcagtaga aaatgcagtt aaaatcgcca agggttatac cggccgttcg
      241 ggaatcgttg ttttcgagaa tgcttttcat ggccgaacac tccttgctat gtctatgacc
      301 cacaaatctt tcccctataa atataaattt gggccttttg cttccgaaat ataccggcta
      361 ccattccttt cacaatatca ttcttcaata caaattgatg acttagaaag agtattgatt
      421 aatctggtga atcctgaaac agtagcggga gtggttattg agccgattca gggtgaagga
      481 ggatttaatg taccaaaaaa cggattttta gaaaagatta aggaattatg ccaaaaatat
      541 gggatgtttt ttatagcgga tgaaatacaa tgcggaatgg gtaggaccgg gaaattcttt
      601 gctgttgaac attggaatat agaaccagat ataatatgtc tggcaaaagc tcttgcctca
      661 ggattgcctt tatctgcagt tatttcccgt aaaaaaatta cagatgctct ccctattgga
      721 actatagggg gcacttatgt aggaaatccg cttggctgtc aggctgccct ggaagtaatt
      781 agaattattc aggaagaaga cctgttaagt aaggctgtca atatcggtaa aattattaag
      841 gatgccttca catcgatgaa agaaaaatat cctattatcg gtgatgtaag aggtcttggg
      901 gcgatggtag ctatggaact ggttaagggt caaaaaagca aggaaccggc tactgaagaa
      961 acagaaaaaa ttattcagta ttgtgttcaa aatggggtgt tgattgcccc ggcaggtcta
     1021 aataaaaatg ttcttagagt tttggtttca ttgaccatat ctactgaaca gctggaagag
     1081 gcgctttccg tactggaaga aggaattgcc cagatataaa aaagctaaat atgaaagagt
     1141 aacactcagg gttaaaagat actattttaa ggagaaaaaa actatagcaa tgtctcaagt
     1201 ctctatcata atgaacaaaa aggatataga cagaaccctg ttacggattt ctcatgaaat
     1261 tattgagaag aacaaaggta tagaaggtct tggcattatc gggataaaaa ccagaggaga
     1321 atacctggct aagaggattg cagaatatat ctatggtatt gaaaagataa atattcctgt
     1381 gggggtactg gatattactc tctatcgtga tgatttgaag gaaaaacaac cccagactat
     1441 tttagttaaa acagaaatac ccttttcgat agaggataag aagatattgc tggttgatga
     1501 tgttctttat actggtagaa ctatcagagc agctatggat gccattgtag acctgggaag
     1561 acccaaatgt attcaattag ccgtattatt agacagaggt catcgggaat ttcccatcag
     1621 ggctgattat gtaggcaaaa atgttcctac ctctcgcaga gaattggtca aagtcagatt
     1681 aggggaggta gatgacctta atcaagtaga aattgttaaa tgaatcctct ttaaccttaa
     1741 gggcgatgag tcattcatat tcatttgtta tctgagacgc gaagatttaa ttactttcaa
     1801 tctcttcaat attttccact aaaataatga agagattgta ttcttttaaa aaattttata
     1861 attagaagca cagtcttaca attcaaccag ggggcttata tgttatatgg taactggggt
     1921 aagatattag aaatagatgt aaaacgaaaa ataacaagga tagtaaagcc atctgaagaa
     1981 atctatgaca agtttttagg tggacgtgga ctgggagtat atttgtatac tcagtatgcc
     2041 agtccattga atggttatgc cctat
//
LOCUS       DAOQWT010000305         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_62614_length_2065_cov_2.938308, whole genome shotgun sequence.
ACCESSION   DAOQWT010000305 DAOQWT010000000
VERSION     DAOQWT010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62614_length_2065_cov_2.938308"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..381
                     /gene="rpmB"
                     /locus_tag="PKJ95_06165"
     CDS             193..381
                     /gene="rpmB"
                     /locus_tag="PKJ95_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNR65857.1"
                     /translation="MAKCEICGKGPQFGKQISHSHRVSNRKWSVNTQRVRVNINNSVK
                     RLNVCTQCLKSGKVNKVI"
     gene            complement(517..1371)
                     /locus_tag="PKJ95_06170"
     CDS             complement(517..1371)
                     /locus_tag="PKJ95_06170"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNR65858.1"
                     /translation="MEIIKAQDIGFCFGVKRAIKKAEQAIKIFPTHEIYMLGEIIHNP
                     QVIKKFKKKGINIIEKICQVPPDKYLITRAHGISKYEENYTKINNIRLIDTACPYVNK
                     LHQIASALKREGYQIVILGDTKHPEIRSLLSYIDNNALVVQSANDVKLKNYKFYGKIG
                     LLSQTTKDIKQFFKITKKIFSHAEELRVFNTICMATRLRQKATEELAKTVDLMIVIGG
                     LNSANTNRLTYLCKKIGVETYHIEDEKQLNLNWFTGKNKIGITSGTSTPDYITRKIIM
                     EIQKLKNR"
     gene            complement(1375..>2065)
                     /gene="cmk"
                     /locus_tag="PKJ95_06175"
     CDS             complement(1375..>2065)
                     /gene="cmk"
                     /locus_tag="PKJ95_06175"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNR65859.1"
                     /translation="ILNNRKTGLIITIDGPAGAGKSTIARELAYKLGFNYINTGDLYR
                     YVTHCAIIKKLDVNNAQEMDLLSREIVNKYTNEYSYDQFISHLKSITEKIHSPEINKK
                     VSFVARHSSVRKNLIPLQRLLAEEGSIVVEGRDIGTVVIPDADIKFFLIADQHTRTMR
                     RFRELREKGYQILFQEIEKEISSRDYIDSKRKTAPLIKPEDAFLIINMNKSISQVVEE
                     MLEIIQKFQRK"
BASE COUNT          624 a          339 c          312 g          790 t
ORIGIN      
        1 tataggcacc agcaataact ctcatatact atttaccttt tttgttgttc tatcctcttc
       61 ctgcctaatt atatatcttt taaaatattt taaaaaaata tattctttaa ctcattttac
      121 ttgaatatag accttatttt atgataaaat aatatttgtt atttttcaag gaaaatactg
      181 gaggaattga ctatggcaaa atgtgaaata tgtggcaaag gtccacaatt tggaaaacaa
      241 ataagtcact ctcatagagt gagtaataga aaatggtcag taaatactca aagagtaagg
      301 gtaaatatta ataactcagt taagagattg aatgtctgta cccaatgttt aaagtctggc
      361 aaggtaaata aggtaatcta aatattttat ttttacctta caggtcttta acgatttatg
      421 taaaacaaat caggtataac ttatttaata aatcaagctg ggtgggaaaa gttagaatcg
      481 ttgatggtgc ctactgcatt attcgggtat atattattat ctgttcttta acttttgaat
      541 ttccattatt atttttctgg ttatgtaatc tggagtagag gtacctgagg taataccaat
      601 tttattcttg cccgtaaacc aatttaagtt aagttgtttt tcatcttcaa tatgataagt
      661 ttctacccca atttttttac ataaataggt caatctattg gtattggcac tatttaaacc
      721 accaatcact atcattaaat ccacagtctt tgctaactcc tctgttgctt tttgacgcag
      781 tctggtagcc atacagatgg tgttgaatac tctcaattct tcagcatggc taaatatttt
      841 ctttgtaatt ttaaaaaatt gtttgatgtc tttggttgtt tgagaaagta aaccaatttt
      901 accataaaat ttataatttt taagttttac atcattagca gattggacta ccagagcatt
      961 gttgtcaata tagctcaata gacttctaat ctcagggtgc ttagtatccc ctaaaataac
     1021 aatctggtac ccttctctct ttagagcact ggcaatttgg tgtaatttat ttacataagg
     1081 gcaggcggtg tctattaatc tgatattatt tatttttgtg taattctctt cgtatttaga
     1141 aataccgtgc gctctggtga taagatattt atccggagga acttgacaaa ttttctcaat
     1201 aatattaata cccttttttt tgaatttctt tataacttga gggttgtgaa taatttctcc
     1261 cagcatgtat atttcatgtg tcgggaaaat ttttattgct tgttctgctt ttttaattgc
     1321 ccttttaact ccaaaacaaa atccaatgtc ttgtgctttt attatttcca ttaattattt
     1381 cctttggaat ttttgaataa tttctaacat ttcttcaact acctggctta tactcttgtt
     1441 catgttaata attaaaaaag catcctcagg tttaattaag ggtgctgttt ttcttttact
     1501 atcaatataa tcacgagaag aaatttcttt ttcaatctcc tggaaaagta tctggtatcc
     1561 tttttcccgg agttccctga accttctcat agttctggta tgttggtctg caataagaaa
     1621 aaattttata tctgcatctg gtattactac cgtaccaatg tctctcccct ccaccactat
     1681 tgaaccttct tcagctaaca gccgttgaag aggtattaaa tttttccgaa cagaagaatg
     1741 acgggcaaca aaagatactt ttttatttat ttcaggacta tgtatttttt cagtaatgga
     1801 ttttagatgt gagataaatt ggtcataact atattcattg gtatatttgt ttacaatctc
     1861 acgggataat aaatccattt cctgtgcatt atttacgtcc agcttcttaa taatagcaca
     1921 gtgggtaaca taacgataca agtcgcccgt atttatataa ttaaaaccca atttataggc
     1981 aagctctcgg gcgatggtac ttttaccagc tcccgctggt ccatcgatag taataattaa
     2041 tccggttttt ctattgttca aaatg
//
LOCUS       DAOQWT010000306         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_62925_length_2059_cov_3.252994, whole genome shotgun sequence.
ACCESSION   DAOQWT010000306 DAOQWT010000000
VERSION     DAOQWT010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62925_length_2059_cov_3.252994"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            560..994
                     /locus_tag="PKJ95_06180"
     CDS             560..994
                     /locus_tag="PKJ95_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNR65860.1"
                     /translation="MDKNKKKSIPRLIMGIGHHLKIRLDKKLSEDNLTISQFRVLAYL
                     WEYGEQRINQKMIHEFLEIKPSSLTKLIKILESKGLIRKEIDPHDSRNKTIKLTEQGI
                     EIKELCLQRIAESEAYILQDFSEKEILTLKELLLRIKKKIRH"
     gene            1114..>2059
                     /locus_tag="PKJ95_06185"
     CDS             1114..>2059
                     /locus_tag="PKJ95_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNR65861.1"
                     /translation="MDEKRIKILRDETPLRAILSLSVPTILAMQVQVFYNLTDTFFVG
                     KLNDPFQVAAVSVAYPIFMILIAFSGVFGFGAASYISRLLGKKDYLMAKKASSTAFYA
                     CLIAATVVTIASLIFISPILHLIGVTTETHRYANDYLSIIFAGSIIMMLNFLVTQLLR
                     SEGAAKVSMYGMFIGTGINIILDPIFILILGYGVKGAAIATLIGQAIALFYYFIFYLK
                     KRSMVSIHWKYLSFRIDILSEIFRVGIPASIHQLTISISQTMGNYMAATYNDLIVAAF
                     GINNRIFSMCIMTLIGLSEGTQPLIGYSYGARKIERMNK"
BASE COUNT          702 a          303 c          334 g          720 t
ORIGIN      
        1 gtatggtgtg aagactgttg ttgttgacaa ataaataatt agaatgaaaa caaagcagcc
       61 tatcagtatt ttattcttca tatagataga gcctccttct tcacattggt ttgctaacaa
      121 tataaaaatt tatacatttc ctttacaatg ttattatata tagaaatttt aaaatgtgct
      181 taaaataaat cttaaatttt tataaaatat caacttttca ttgacaaaat attgttcatt
      241 attataatgt atttagctag ctaaatatct aattaatatt taacaattgt gggctttaca
      301 ttgtctataa ttttacgttt ttaagtttta attcattgtg gaagattgat atttttcaaa
      361 taaaagttta ttatctaatt tttgttaata acttgagcca gttttttgaa ggaataattg
      421 taagtacaat gttttttata gttcaatttt ttaaaaatag aatacaagaa tgtcattaga
      481 aaaaatatcg ttaattaata tattcttaac caaaattatt tcataaactt taagcttatt
      541 ggtgatttga ggtttttaaa tggataaaaa taagaaaaag agtatcccca gactaattat
      601 gggaattggc catcatttga agatacgtct ggataaaaaa ttaagtgaag ataatttgac
      661 catctcccaa ttcagggtgc tggcctattt atgggaatac ggtgagcaga gaattaatca
      721 aaaaatgatt cacgaatttt tggaaatcaa gccctcttct ctgactaaat tgataaaaat
      781 actggaatca aaaggactga ttagaaaaga aatcgatcca catgattctc gaaacaaaac
      841 tataaaacta accgaacaag gcattgaaat aaaggaatta tgtttacagc gcattgccga
      901 atcagaagca tatatcttgc aggatttttc tgaaaaggaa attcttaccc taaaagagct
      961 cctgctcagg attaagaaaa aaatcaggca ttgatatctc ctttataata tttattccca
     1021 aaattatatt tgctctttct tttcctataa gcaaccaatg tttttaatat tgtaattata
     1081 ttttatttga ttcagatttg aggaattgaa gaaatggatg aaaaaagaat taaaatacta
     1141 agagatgaaa ctcctttacg ggcaattttg agtctatctg tgcctacaat actggctatg
     1201 caagttcagg tattttacaa tcttaccgac accttctttg taggaaagtt aaatgaccct
     1261 tttcaggttg ccgcagtatc tgttgcctat cccatattta tgattctaat tgccttcagc
     1321 ggggtattcg gatttggagc agcatcatat atttcccgat tattgggtaa aaaagattat
     1381 ttaatggcta agaaagccag ctctacagct ttctatgcct gcctcatagc ggctactgta
     1441 gtaacaattg caagccttat tttcatttcc cctattttac atctgatagg ggttaccacg
     1501 gaaacacata gatatgccaa tgattatctg tcaataattt ttgcaggaag tattattatg
     1561 atgctaaatt ttctggtcac ccagcttctc aggtcagaag gagcagctaa ggtatctatg
     1621 tatgggatgt ttataggaac tggaataaac ataattttag atcctatttt tatactgata
     1681 ttaggctatg gggtaaaagg ggcggcaatt gccactctaa taggacaggc aattgcctta
     1741 ttctactatt ttattttcta cctgaaaaag aggagtatgg tctctattca ctggaaatat
     1801 ttatctttta gaatagatat cctgtctgaa atttttagag tcggtattcc ggcctcaata
     1861 caccaattaa ccattagtat atcccagaca atgggaaatt atatggctgc aacttataat
     1921 gacctgattg ttgctgcttt tggcattaac aatcgcattt tttccatgtg tataatgact
     1981 ttaattggat tatcagaagg tactcaacct ttaattggat atagttatgg tgccagaaaa
     2041 attgaacgaa tgaataaaa
//
LOCUS       DAOQWT010000307         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_63202_length_2054_cov_2.967984, whole genome shotgun sequence.
ACCESSION   DAOQWT010000307 DAOQWT010000000
VERSION     DAOQWT010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63202_length_2054_cov_2.967984"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2054)
                     /gene="secA"
                     /locus_tag="PKJ95_06190"
     CDS             complement(<1..>2054)
                     /gene="secA"
                     /locus_tag="PKJ95_06190"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008564 - protein-exporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNR65862.1"
                     /translation="TEQRKEAYRSDVVYGTNNEFGFDYLRDNMVINPEEIVQYEHEFA
                     IIDEVDSILIDEARTPLIISGPTEESTSKYFQIDKLIYQLQEERDYKINEKEKTAYLT
                     EEGVTRMERLLKVDNLYAQNNLHLQHHIIQALKAHKLFRRDVDYVVKNGEVIIVDEFT
                     GRLMFGRRYSDGLHQAIEAKENVAIARESQTLATITFQNFFRLYRKLAGMTGTAKTEE
                     NEFIEIYNLPVVVIPPNRDLIRHSFPDVIYKTEREKFVAAVNEIKELHQQGRPILVGT
                     VSIEKSEKLSKLLNKEYIEHNVLNAKNHEREAEIVAEAGQRGKVTISTNMAGRGTDIV
                     LGAGVAEMGGLHVIGTERHESRRIDNQLRGRSGRQGDPGSSRFFLSLEDDLLRLFGSE
                     RIAGFMDKLGVEDGIPIEHTFITRSIEGAQKRVEGRHFEIRKQLLEFDNEMEFQRKIV
                     YEQRKTVLLSNDIKNIILDMVEDTVSSMVQRYTSKDVYPEEWNLLALKDHFYETFGMA
                     LNFDKDIVNLTIEILQEDLLERAREGYQMKEKELGPPLMRQIEKMILLRIVDREWKDH
                     LKRLDDLKQGIGLRAYGQKDPLIEYHFEAHNMFQNMIENIKEDGIKFIYRVKIQQKPQ
                     TIEKSQETEVQADLSGKGVVKGEQPKKAMAKVALSTKKIGRNDPCPCGSGLKYKYCCG
                     KN"
BASE COUNT          543 a          455 c          284 g          772 t
ORIGIN      
        1 ttattcttcc cacaacaata cttatattta agtccactgc cgcagggaca gggatcattt
       61 ctaccaattt ttttagttga tagggcaact ttcgccattg ctttctttgg ttgttcacct
      121 tttactactc ccttgcctga taaatctgcc tgcacttccg tttcttgact tttttctatg
      181 gtctggggtt tttgttgtat cttcactctg taaataaatt ttattccatc ttctttaata
      241 ttttcaatca tattttgaaa catattatgt gcttcaaaat gatattcaat taagggatct
      301 ttttgcccat atgcccttaa accaattccc tgttttaaat catctaatct tttaaggtga
      361 tctttccatt ctctatcaac tattcttagc aagatcatct tctcaatctg tctcattaaa
      421 ggaggaccga gctctttttc tttcatttga taaccttctc tggctctttc taataaatcc
      481 tcctgcagta tttcaatagt taagttaaca atatctttgt cgaaattaag agccattccg
      541 aatgtctcat aaaagtggtc tttcaatgcc aataaattcc attcttcagg gtatacatct
      601 ttactggtat atcgttgcac catactgctc acagtatctt ccaccatatc caggataatg
      661 tttttaatat cattactcaa taaaacagtt tttctctgtt cgtagacaat ttttctctga
      721 aattccattt cattatcaaa ttctaataat tgtttcctga tttcgaaatg tcttccttca
      781 actctttttt gtgccccttc tatagaacga gttataaagg tatgctcaat aggtatgcca
      841 tcttctactc ccaatttatc cataaaacca gcaatacgtt ccgaaccaaa taagcgtaag
      901 aggtcatcct ccaatgataa aaagaaacgg gaagaaccag ggtctccctg tctgccagat
      961 ctacctctta attggttatc tatccttctg ctctcatgcc gctcagtacc gatgacgtgc
     1021 aatcctccca tttctgccac accagcacct aaaacaatat cagtacccct gccagccata
     1081 ttggtagaga tggtcacttt acctctttgg ccagcctcag caacaatttc tgcttctctt
     1141 tcatgatttt tagcattcaa gacattgtgt tctatatact ctttatttaa caatttactc
     1201 aatttttctg atttctcaat tgatactgtg ccaactaata tcggtcttcc ttgctgatgt
     1261 aattctttaa tttcgttcac agctgccaca aatttctccc tttccgtttt ataaatgaca
     1321 tccgggaagc tatgtcttat taaatctctg tttggaggta ttactacaac cggaaggtta
     1381 tatatttcaa taaactcatt ttcctctgtt tttgctgtac cggtcattcc tgctaatttt
     1441 cgatacaatc taaagaaatt ttgaaaggtt atagtggcaa gagtttggct ttcacgggca
     1501 atagcaacgt tttctttagc ttcaatggct tgatgtaaac catcgctata ccttctacca
     1561 aacataaggc gcccggtaaa ttcatctaca atgattactt ctccattttt taccacataa
     1621 tctacatctc ttcgaaataa cttatgtgct ttgagagcct gaataatgtg gtgctgtaag
     1681 tgtaaattat tttgtgcata taagttatca actttcagca atctttccat ccgggttact
     1741 ccctcttcag tcaaataggc agttttctct ttttcattaa tcttataatc acgttcttct
     1801 tgtaattggt aaattaactt atctatttga aaatacttac tggttgattc ctcggtgggt
     1861 ccagatatga ttagtggggt tctggcttca tcaattaaaa tactgtctac ttcgtcaatt
     1921 atggcaaatt catgctcgta ctgcactatt tcctcaggat ttattaccat attatctctt
     1981 aaataatcaa atccaaattc attatttgtc ccgtaaacta catctgaccg gtaagcctcc
     2041 ttcctttgtt cggt
//
LOCUS       DAOQWT010000308         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_63303_length_2052_cov_3.008012, whole genome shotgun sequence.
ACCESSION   DAOQWT010000308 DAOQWT010000000
VERSION     DAOQWT010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63303_length_2052_cov_3.008012"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKJ95_06195"
     CDS             complement(<1..623)
                     /locus_tag="PKJ95_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR65863.1"
                     /translation="MFFKFDPSMIPIMVLGMNSEQYKLNELENFARDIVKPRLERLEG
                     IALASVSGGLKREILVSVDNRRLRANNLTLDHVVMALRSENINLPAGTLREGNIDFLI
                     RTLGRFQSIADIENVIISNMQGNQISLKEIARVSDTFKEQTVEAYINGFAGISFSLQK
                     ESGTNSVLVANRVNKELKTINNILPGDARLMVVFDSSDFIKKTIAEVG"
     gene            complement(613..1002)
                     /locus_tag="PKJ95_06200"
     CDS             complement(613..1002)
                     /locus_tag="PKJ95_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR65864.1"
                     /translation="MNLPRFSIRRPVTILMLFIALIAIGIVSYGRLGLDLLPDMSFPI
                     SAVMVSYPGVAPEEIETLITIPLEEVLSTIHRIDTISSVSREGNSLLLLSFQWGTNMD
                     IASLEIREKVDQIKGILPEGASDPSVF"
     gene            complement(999..>2052)
                     /locus_tag="PKJ95_06205"
     CDS             complement(999..>2052)
                     /locus_tag="PKJ95_06205"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR65865.1"
                     /translation="KSIGQAKEVLVEEGDLVKKNDVLMRLDDEQIRLQVAQAKANLDV
                     AQASLERIKAGARPQEINQAEAAVRQAKVNLDSAEENYQKMRKLFSEGAVSEQQHDQA
                     KNHFEIAQAQYQVASESYKLVTEGASPEDIKTVEAQVRQAQSALELAQSQLGNTIIRA
                     PISGSVTALSVKTGELVSSAMPLLSIMDKSELYLKTGISEKDIAAITIGQRSEVFIDA
                     FSGEVFSGEVTSKGAIVDPLSKTMEIKIKIVKPHIDITPGMFARANILIAQDPDTLVI
                     PSTALSRRLDGLFVFVLDKNKETIQKKPVIIGITHSNQVEVIDGLQENEIIVILGNIA
                     LEEGERVRVTNRENIE"
BASE COUNT          579 a          473 c          358 g          642 t
ORIGIN      
        1 cctacctcgg caatagtctt ctttataaaa tccgaggaat caaaaactac catcaggcgg
       61 gcatcaccgg gtagtatatt gttaatagtt tttaattctt tgtttactct attggctacc
      121 agtaccgagt ttgttccgga ttctttttgt aggctaaaag agataccggc aaaaccattg
      181 atataagcct caacagtctg ctctttaaag gtatctgaaa cccgggcaat ttcctttaag
      241 gatatttgat tcccttgcat attggatatg attacatttt caatatctgc aatactctga
      301 aagcgtccca atgtcctgat taaaaaatca atattgcctt cccttaaggt acctgcaggc
      361 agattaatat tctcactcct taaagccata accacatgat ccagggttaa gttgttggct
      421 ctcagtcttc tattatcaac agaaactaat atttcccgtt tcaatccacc acttactgaa
      481 gcaagggcaa ttccttccaa tctctctaaa cgaggtttaa ctatatcccg ggcaaaattt
      541 tccagttcat taagtttata ctgctctgaa ttcattccca gtaccataat gggaatcata
      601 gatgggtcga atttaaaaaa cactgggatc acttgcccct tcaggcagaa tacctttaat
      661 ctggtcaacc ttctccctga tttccaatga ggcaatatcc atatttgttc cccattgaaa
      721 ggataagagc aaaagagaat ttccttccct ggagacagaa ctgatggtat caatacgatg
      781 aatggtgctg agaacttctt ccaaaggaat ggtaatcaat gtttctattt cttccggagc
      841 aactcccgga taagagacca ttactgctga tatgggaaag gacatatcag gcagtaaatc
      901 cagacccaat cttccataag atacaatacc aatggcaatt aaggcgatga aaagcattaa
      961 gatggtcaca gggcgccgga tagaaaatct gggtaaattc attctatatt ctccctgtta
     1021 gttactctca ctcgctctcc ttcctctaaa gcaatatttc ctaatattac aataatttca
     1081 ttttcctgca agccatcaat cacttccacc tggttactat gtgtgatacc gataataaca
     1141 ggtttttttt gtatagtttc tttattctta tctaatacaa aaacaaataa tccatccaat
     1201 cttctgctta acgccgtaga aggtataact aaagtatcag gatcttgcgc aataagaata
     1261 ttagccctgg caaacatacc gggggttata tcaatatggg gcttaacaat ttttatcttt
     1321 atttccattg tcttggaaag ggggtccact attgcccctt tactggtaac ctctccagag
     1381 aatacttccc cggaaaaggc atcaataaat acctccgatc gctgacctat ggtaatggca
     1441 gcaatatctt tttctgagat gcccgtttta agatatagtt cactcttatc cataattgat
     1501 aacaagggca ttgccgaaga aaccagttcc cctgttttca ctgaaagtgc agtaacactt
     1561 ccgctgatgg gcgctctgat aatggtattg ccgagctggg actgagctaa ttccagagca
     1621 gattgagcct gtctgacctg tgcttcaaca gttttaatgt cttcaggaga ggctccttca
     1681 gttaccagtt tatagctttc tgaagcaacc tgatattggg cctgggcaat ttcaaaatga
     1741 tttttagcct ggtcgtgctg ttgctctgaa acggcaccct cagaaaacaa ttttcgcatc
     1801 ttctgataat tctcttcagc actatccaga tttacctttg cctggcgaac tgctgcttct
     1861 gcttgattaa tctcctgtgg tcttgcccct gctttaattc tctctaaaga agcctgcgcc
     1921 acatccaaat ttgctttggc ctgggctacc tgaagccgga tttgttcatc atccaaacgc
     1981 attaaaacat catttttctt taccaggtca ccttcttcta ccaaaacttc cttagcctgg
     2041 ccaatactct tg
//
LOCUS       DAOQWT010000309         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_63422_length_2049_cov_3.144935, whole genome shotgun sequence.
ACCESSION   DAOQWT010000309 DAOQWT010000000
VERSION     DAOQWT010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63422_length_2049_cov_3.144935"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKJ95_06210"
     CDS             <1..508
                     /locus_tag="PKJ95_06210"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HNR65866.1"
                     /translation="FIVTIYFWWSSKEEKKALLEDIKKPILFEHALLVLILIMFGIIY
                     ITRSGNFSILTISNIEEKIRLFLEKYLIARPRNKEFLIGYPLLSLAIAMNHLDIHYLK
                     IPVIAVGTIAPITVVNTFCHIHTPFLFSLLRTFNGYWLGLFFAILFPAILFFFLKIIR
                     ICLNVEKK"
     gene            492..1646
                     /gene="csaB"
                     /locus_tag="PKJ95_06215"
     CDS             492..1646
                     /gene="csaB"
                     /locus_tag="PKJ95_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HNR65867.1"
                     /translation="MLKKNRNLKSIIISGYFGFGNCGDEAILMAMIQQLSQIVSKENI
                     VVLSQNPKKTKMLYGVNSVFRLNPFLIFRQMKKCAVFISGGGGLLQDVSGKGLSIIYY
                     LSLILLARLFKIPNVIYGQGIGPIRREINRKILRLILSKTNLIIVRDEQSQLLLQQIG
                     IEKESVLVYADPSFLLKKEQLSSEIEKYYFLGPRYGKSINKMTIGVIIRNCKEIEQDY
                     NQKVLQLAKVADHLITKYQSRLIFIPFQIDNDLTLLNDVIKQMKNSSVDCLEREINPA
                     QMLSFFSELSLTIGMRFHAILFSTINNNPFIAIDYDPKVRNYVNSLELPELLLNLNQL
                     TIKYIDYKLQYINDNWEKIKSILDTNTKQFQGKAEAGMKELLSFIKERIL"
     gene            1678..>2049
                     /locus_tag="PKJ95_06220"
     CDS             1678..>2049
                     /locus_tag="PKJ95_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR65868.1"
                     /translation="MFHVFKTFPNKIHALWDINLHVKKGEFVFLVGPTGAGKTTLLKL
                     VYRGILPSKGQVIVDGVNLSRLKLSLVPYLRREIGIVFQDFKLLYDRNVFENVAFSLR
                     VVGTKNIIIKQKVNEVLDIIGL"
BASE COUNT          709 a          279 c          344 g          717 t
ORIGIN      
        1 ttttattgta accatatatt tctggtggag cagtaaagaa gaaaaaaaag cattgttaga
       61 agatattaaa aaaccaattc tgtttgaaca tgctttgctg gttctgattt taataatgtt
      121 tggaataata tatattacac gttcaggaaa tttttcaatt ttaacaattt caaatataga
      181 agaaaaaatt agactatttc ttgaaaagta tttaattgcc cggcccagaa acaaggaatt
      241 ccttattggt tatcccttgt tatcactggc tattgcaatg aaccatttgg atattcacta
      301 tctaaaaatt ccggtaatag ctgtgggtac catcgcacct attactgtcg taaacacctt
      361 ttgtcatata cacactcctt tccttttttc attattaaga acttttaatg gttattggct
      421 tggattattt tttgctattt tatttcctgc tattttgttc ttttttttga aaataattag
      481 gatttgtctg aatgttgaaa aaaaatagaa atctcaaatc aattataatt tcgggctatt
      541 ttggttttgg aaattgtgga gatgaagcca ttctgatggc aatgatacaa cagctatcac
      601 aaattgtttc aaaagaaaat attgttgtct tatcgcagaa tcctaaaaaa actaaaatgt
      661 tatacggagt aaattctgtt tttcgtttaa acccttttct tattttcaga caaatgaaaa
      721 aatgtgctgt ttttatcagt ggaggtggcg gtttattaca ggatgttagc ggcaaagggt
      781 tgagtattat ctattatctt tccttaattc ttttagccag actgtttaaa atacctaatg
      841 ttatatatgg gcagggtata gggccaataa gaagagaaat taacagaaaa attttaaggt
      901 tgattctatc caaaacaaat ctaattatag tgagagatga acaatcacag cttctcctac
      961 agcaaattgg tatcgaaaaa gaatcagtcc tggtttatgc tgacccctct tttttattaa
     1021 aaaaagaaca attatcttct gaaatagaaa aatattattt tttgggacct cggtatggaa
     1081 aatctattaa caagatgact attggggtga taattagaaa ttgtaaagag atagaacaag
     1141 attataatca aaaggtttta cagttagcca aagtagcaga ccatctaatt acaaaatacc
     1201 aatccagact tatttttata ccttttcaaa ttgataatga tttgacttta ctaaatgatg
     1261 taattaaaca aatgaaaaat tcttctgttg attgtctgga acgggaaata aatccagctc
     1321 aaatgctatc ttttttttct gaattatctc taacaattgg gatgagattc catgcaattc
     1381 tattctcgac tattaacaac aatcccttta tagcaattga ctatgatccc aaagtcagaa
     1441 attatgttaa ttccctggaa ttgccagaat tattattaaa tctaaatcaa ttaactatta
     1501 aatatattga ttataagtta caatatatta atgataattg ggaaaagatc aaatctattt
     1561 tggatactaa cacaaaacag tttcagggca aggctgaggc tggaatgaaa gaattactat
     1621 cttttataaa agaaaggatt ttataattag aaaggaaatg aagaactttt gatacggatg
     1681 tttcatgttt ttaaaacttt tcctaataag atacatgccc tatgggatat taatcttcat
     1741 gttaaaaagg gagaatttgt ttttttagtg gggccaaccg gcgccggtaa aacaactttg
     1801 ttgaaattag tttaccgggg gatacttccc agtaaagggc aggttatagt tgatggggtt
     1861 aatctatccc ggctaaaact atctttagtg ccttatttaa gaagagaaat tggtattgtt
     1921 tttcaggact ttaaactgtt atacgatagg aatgtatttg agaatgtggc atttagctta
     1981 agagtagttg gtacaaaaaa tattattatc aaacagaagg taaacgaagt tctggatatc
     2041 attggactt
//
LOCUS       DAOQWT010000310         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_63876_length_2040_cov_2.928967, whole genome shotgun sequence.
ACCESSION   DAOQWT010000310 DAOQWT010000000
VERSION     DAOQWT010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63876_length_2040_cov_2.928967"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PKJ95_06225"
     CDS             <1..651
                     /locus_tag="PKJ95_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65869.1"
                     /translation="LCPRPGASAPSKVEFSSLASVLPAVISAPSPQQNLTAPIGAPVT
                     APPTAGIKPAIIEKGKAILQEKGFTAPQIQAITQRITDSIRKHLPKKPDSYFLTAVKN
                     EKIKTSVTIPPALGNNTTGSPDPQARQIPPAPIGAMVQVQNNSFTETVNRWEQEKKKD
                     SPEETIKKLMELSPDDLYRVILDVDNSPASKFISLPEIKASLYIAEFKKRHPEVKV"
     gene            complement(1310..>2040)
                     /locus_tag="PKJ95_06230"
     CDS             complement(1310..>2040)
                     /locus_tag="PKJ95_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65870.1"
                     /translation="PEVARLTGKTVNTIYRYIRNDKLRYNRVIYQGREVYRIEKKELQ
                     RVFKCNISGDISDISEDITGDISSDISKDTSDISEDKIQRAIEKAISQQQAVFLKPIE
                     DQALYRLGRMEQENSFLKAKVETLLQELDQYKSLPGPADIEKMQKENQEKEKDLIIQI
                     ELERKEKTDLLSREKETEEKHRRELSELETRQEEQNELHREELQQTWKQAEDEQKNYL
                     ATIEELKKRLQEEEKRPWYRRLFS"
BASE COUNT          575 a          444 c          346 g          675 t
ORIGIN      
        1 ctatgccccc ggcccggtgc cagtgctccc agtaaagttg aattctcttc cctggcatct
       61 gttttgccgg ctgtaatttc tgctccatca ccacaacaga atctcactgc tcccataggt
      121 gcccctgtaa cagctccccc cactgcaggc ataaaacctg ccataataga aaaaggaaaa
      181 gccatcttac aggaaaaagg tttcaccgct ccacagatac aggcaatcac tcagagaatt
      241 accgatagta taagaaaaca tttacctaaa aagccggatt catatttcct gactgctgtt
      301 aagaatgaaa aaataaagac ctctgtcact attcccccag ccctggggaa taataccact
      361 ggatcacctg atccacaggc ccggcagatt cccccggctc ccataggtgc catggtacag
      421 gtgcaaaata attccttcac tgagaccgtc aatagatggg aacaagaaaa gaagaaggat
      481 agccctgaag aaacaataaa aaaacttatg gaattaagcc ctgatgattt atacagagtg
      541 attctggatg tagataactc acctgccagt aaatttatat cactgccgga aataaaggca
      601 tctctctata tagcagaatt caaaaaaagg catcctgaag taaaagtata gttatcaaaa
      661 atatcccttt atgagaatgg ctctaaaatg ctctgtaatg aattgaaaat ggaaaaggca
      721 taaaactata actttaatat tctgtgaaga atttcatgag gttttgcttc aatttttgct
      781 atgccggttt aaagggtcgt gagaggataa aaaaattcat cagtcatgta tcgagaattt
      841 tgaaagaata tctgttttaa aaacgcttcc tattttgcgt tttaacgcat ttaaattaag
      901 ctttgataca tttctgtcat tctggcataa gtcatgctaa aactgccatt cttgccccta
      961 ttttaaacgt tctgataaaa taccttgttt caatctggta tttttataat attactgctt
     1021 atgatagaat tatttatatt atttataaac tttattaatt ttcaaaagta aaatgtattt
     1081 ttgcccatcc cctccaaaaa gccggtagaa aattcattta tatttttagt atgaaaacga
     1141 ttaatgtaaa aaacagccat aaggaattct cactatgggg tatattttga gggaaacagg
     1201 gtaaatgtta cgtaggaatt tttttatctg agtgttttcg cccattcccg cgaaaagtac
     1261 aatagaatgt tttcggtcta aacaggggga atgtttctta agggtgcagt caagaaaata
     1321 accgccggta ccatgggcgt ttttcttctt cctgcaatct cttttttagc tcctctatag
     1381 tagcaagata attcttctgt tcatcttctg cttgtttcca tgtctgctgc agctcctccc
     1441 ggtgaagttc gttctgttcc tcctgtcggg tctccagttc tgacagttct ctcctgtgtt
     1501 tctcttctgt ctccttctcc ctggataata gatctgtttt ctctttccgc tctaattcga
     1561 tttgtataat caggtctttc tccttctcct ggttctcctt ctgcatcttc tctatatctg
     1621 ccgggcctgg taatgattta tactgatcca actcctgaag taatgtttcc acttttgcct
     1681 ttaagaagga gttctcttgc tccattcttc ccagacggta aagggcctgg tcttctatgg
     1741 gctttaaaaa tactgcctgc tgttgactta tggccttttc aatagccctt tgtattttat
     1801 cctccgatat atcggatgta tcttttgata tatcactcga tatatcacct gttatatcct
     1861 ccgatatatc ggatatatcg cctgatatat tgcatttaaa aactctttgc aattccttct
     1921 tttctatcct gtaaacctct ctaccctgat atatcaccct gttatatcgc aatttatcat
     1981 tcctgatata tcgatatatc gtgttaactg tcttgcctgt cagacgtgcc acttctggaa
//
LOCUS       DAOQWT010000311         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_64211_length_2033_cov_3.365521, whole genome shotgun sequence.
ACCESSION   DAOQWT010000311 DAOQWT010000000
VERSION     DAOQWT010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64211_length_2033_cov_3.365521"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PKJ95_06235"
     CDS             <1..875
                     /locus_tag="PKJ95_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNR65871.1"
                     /translation="FKFVFGYEKNERLLIALLNAILALEGAEKIVKLTFLNTFNLQEY
                     LKDKLSTLDVKAEDNTGRRLNIEMQVEPDRAYVNRVIYYHDKLYTGQLKEAEPFEELH
                     KAISISILNFILFKEERDLHNIYRYLNIKSKKELTDVKELHFIELPKFKKDKPKKLQS
                     RFERWLHVLKFGELYAEDIDNLPEELKQEEEIVMALQEMVRASNDETVRQILEMRSKA
                     RHEEASRLFQAKQAGKEEGKKEGKEEGKKEGIKEGIEKRNIEVAKNMLKLNLPVDTIS
                     QATGLTASEIEHLK"
     gene            901..1125
                     /locus_tag="PKJ95_06240"
     CDS             901..1125
                     /locus_tag="PKJ95_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012152190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65872.1"
                     /translation="MQEEQSLTEEMVLQILEMRSKARHEEASRLYQAEQRGIEKRNIE
                     IAKNLLKLNFHVEAIFQATRLTAGEIEKLK"
     gene            complement(1389..1724)
                     /locus_tag="PKJ95_06245"
     CDS             complement(1389..1724)
                     /locus_tag="PKJ95_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR65873.1"
                     /translation="MSKKEEWQILRDQGSTFEDIAQKYNTSKQLVWQTLDIAGAPRKT
                     KHSAHYQDWENLYNQGISIPDIAKKYNCSTSTVHNYLKKKVSMDASKSALKAWKKKKK
                     NGRKKKEIE"
     gene            complement(1845..>2033)
                     /locus_tag="PKJ95_06250"
     CDS             complement(1845..>2033)
                     /locus_tag="PKJ95_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65874.1"
                     /translation="HYPVCNIQFQATFRSLPFYFSLHREQSCFLSTPYVLLADGSGRP
                     YPPRVFCFFPLSIIYIII"
BASE COUNT          741 a          264 c          402 g          626 t
ORIGIN      
        1 tgttcaagtt tgtgttcgga tatgagaaga atgaaaggtt attaatagca ctacttaatg
       61 ctatacttgc cctggaaggt gcagaaaaga tagttaagct gacttttcta aatacgttca
      121 atttacagga atatcttaag gataagctct caactcttga tgttaaagca gaagataata
      181 ccggcaggag attgaatatt gaaatgcagg tagagccgga tcgggcatat gtaaatagag
      241 taatttatta tcatgataag ttatatacag gtcagctaaa ggaagctgaa ccttttgaag
      301 agttacataa agctatatct atttctatat taaattttat tctttttaaa gaagaaagag
      361 atttgcataa tatttatcgg tatttgaata ttaaatcaaa gaaagaatta actgatgtta
      421 aggaattgca ttttatagaa cttccgaagt ttaaaaagga taagccaaag aagttgcaat
      481 cacgatttga gcggtggctg cacgttttaa aattcggaga actttatgca gaggatatag
      541 ataatctccc ggaggaattg aaacaagagg aggagatagt aatggctttg caagagatgg
      601 taagggcatc taatgatgaa actgtaaggc agatacttga gatgagatct aaggcccgtc
      661 atgaagaggc ttccaggttg tttcaggcta aacaggcggg gaaggaagag ggaaaaaagg
      721 agggcaaaga agaaggaaaa aaagaaggca taaaagaagg gatagaaaaa agaaatattg
      781 aagttgcaaa aaatatgtta aagttaaatt tacctgtaga tacaatatct caagctactg
      841 gattaactgc cagtgaaata gaacatttaa aataacaatt aatattctgg agaagatacc
      901 atgcaagagg agcaatcatt aactgaagaa atggtgctgc agattcttga gatgagatct
      961 aaggctcgtc atgaagaggc ttcaaggttg taccaggcag agcagagagg tattgagaaa
     1021 agaaacattg agatagcaaa aaatctttta aaattaaatt ttcatgtaga agcaatattt
     1081 caggcaactc gattaactgc cggtgaaata gaaaaattaa aataacggaa tttttgtctc
     1141 tggaaaattc ttgatagttt cagtcagtta tttttgtaaa agccttgatt ttactggttt
     1201 ttatatggtt tataaaggat gaatttttta taatttattg gattgcaaga aacccttgca
     1261 atcacagggt ttaaattttt aaaaatgtca ttaaagaagc atttaacgac atttttagat
     1321 aatttcgttt acaaaggatg aacataagac taaatttatt agaaaactca acaatagtaa
     1381 cctcttttct attcaatttc ctttttcttt ctgccattct tttttttctt tttccaagct
     1441 ttaagagcag atttactggc atccatagaa acttttttct ttaaataatt atgaactgtt
     1501 gaagtagaac aattatattt tttcgctata tcaggaatac ttataccctg gttgtataaa
     1561 ttttcccagt cttgataatg ggcagaatgt tttgttttac ggggagctcc ggcaatatct
     1621 aatgtctgcc agacaagctg cttacttgta ttatattttt gggcaatatc ctcaaaggtt
     1681 gatccttgat ctcttaatat ctgccattct tcttttttac tcattacatt caccttcttt
     1741 ggaataatta taatatatct aattcgaaaa gtaaatacaa ttttacaaat aaaaacaata
     1801 aaattatata aaaatattgt aaaatagtat tgacatatcg aatattatat gataatatag
     1861 ataatagaga gagggaaaaa acagaacaca cggggcgggt agggtctgcc tgaaccatca
     1921 gccagaagga catagggggt tgataggaag caagactgct cacggtgcaa tgaaaaatag
     1981 aaaggaagtg atctaaatgt ggcttggaat tgaatattac ataccggata atg
//
LOCUS       DAOQWT010000312         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_64345_length_2031_cov_2.468117, whole genome shotgun sequence.
ACCESSION   DAOQWT010000312 DAOQWT010000000
VERSION     DAOQWT010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64345_length_2031_cov_2.468117"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1325
                     /locus_tag="PKJ95_06255"
     CDS             135..1325
                     /locus_tag="PKJ95_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNR65875.1"
                     /translation="MKEINRESEKGEISELEILFLIATSNDGTIYFSDYPRKETKIAK
                     DGKTKIKKWKQYLHLRILNLIFEKQEEGKSIFRSNLALDSFQENQGSYGTEVGRIGIN
                     NYLAIKNTLTMYDWIKGKELREMEEDYGILGGNLQKMGEGFSWLADALSSLAEKEGWK
                     EVRADELAKIRELSVRLIHGIGSEGLTLAKLNIPGLTRGYIQRLIQEGYNNEQCLIEL
                     SDNQLRQILPELLIKRIRKHINSKPCCLDSDTNLNTNWELGKEKDKEIDEECSKATII
                     LNPNRPDRIIFLDNEIAVNKIGFQLISLLAKNEGKVLSYDQIIDTLWPSDEDATYHRL
                     WYHLARLRKEMQGVIRNNKDKNESKNKDNTLLADSGLKSKVFRVIPGIGLMLSPELQV
                     KWTE"
     gene            1581..>2031
                     /locus_tag="PKJ95_06260"
     CDS             1581..>2031
                     /locus_tag="PKJ95_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65876.1"
                     /translation="MEILKQSISNISPITGEFTNQSLERDFQLHSFQEFNDYSRKYIL
                     TFGFLFFLFIIPDYFLIKDRYQLTTIFLIRILYLLMTFLYYSHLPHLKLYLNLFSTIY
                     EFAGIVCFWILFFCDKHPNIFFYQQGLIILILAIFLILPNRYVNKIFL"
BASE COUNT          715 a          303 c          378 g          635 t
ORIGIN      
        1 attgaagagc agcagtgaga taaagaatga gttgataaaa aaggctgata cggcactcta
       61 tttttcaaaa aacagcggaa gaaataagac cactaagttt gaaaaatatc ttgagataaa
      121 aaagagtgtt atgagtgaaa gaaattaata gagagagtga aaaaggagaa attagtgaat
      181 tggaaattct ttttctaatt gcgaccagta atgatggcac catctatttt agcgattatc
      241 ccagaaagga gactaaaata gcaaaagatg gcaaaacaaa gattaaaaaa tggaaacagt
      301 atttgcattt gcgtatccta aatctaatct ttgagaaaca ggaggagggt aaatccatct
      361 tccgaagcaa tttagctctt gattcttttc aggaaaacca gggttcttat ggcactgaag
      421 ttggtagaat tggtattaac aattatcttg ccataaaaaa taccctgact atgtatgact
      481 ggattaaagg taaagagttg agagagatgg aagaagatta tggcatttta ggtggaaact
      541 tacagaagat gggcgaaggt ttttcctggc tggcagatgc cttatcctct cttgctgaaa
      601 aagaaggatg gaaagaggta agagcggatg aactggcgaa aatcagggag ctttcagtac
      661 gactgataca tgggattgga tcagaggggc ttactctggc aaaacttaac atacctggct
      721 taaccagagg ttatattcag aggttaatac aagaaggtta taacaacgag caatgtctca
      781 ttgaattatc ggataatcag ttgaggcaga ttctaccgga acttttaata aaaagaataa
      841 ggaaacacat taattccaaa ccttgctgtt tagattcaga taccaattta aatacgaatt
      901 gggaattggg caaggaaaag gataaagaaa tagacgagga gtgcagcaag gcgactatta
      961 tcctcaaccc aaacaggcca gaccggatta ttttcctgga taatgagata gcggtaaaca
     1021 aaattggctt ccagcttatc tcacttttgg ccaaaaatga ggggaaagta ttatcttatg
     1081 accagattat cgatacgctg tggccttcag atgaagatgc cacctaccat cgcctctggt
     1141 accacctggc cagactgaga aaagaaatgc aaggagttat cagaaataat aaggataaaa
     1201 atgagagtaa gaataaggat aacacccttc tcgctgatag tgggctgaag agtaaagtgt
     1261 tcagggtaat tcccggcatc ggcttaatgc tcagccctga attgcaggta aaatggactg
     1321 aataatttct ttttttgact tttatgactt ttaataaata attatatata cattattttt
     1381 aaaatatctt gtctttttaa ttcttaccac tataatatag tgcattaatc tctttcttaa
     1441 tagtaataaa tatagtagta attattgata atataagtat tttacagaaa gaatgggatt
     1501 gactcatatt ttttgataaa aactattctt tttcatgaat tagtaaaata tagatataca
     1561 aatatacgga atatttaaag ttggaaatat taaaacaatc tataagtaat atttctccta
     1621 taacagggga atttaccaat caatctctgg aacgtgattt ccagttacac agctttcagg
     1681 aatttaacga ttattcgcgt aaatatatac taaccttcgg ctttctattc tttttattta
     1741 ttattcctga ctattttctt attaaggata gataccagct gactactatc ttcctgataa
     1801 ggattcttta tctgttaatg acattcttat attattctca tttaccacat ttaaaactct
     1861 atttaaatct tttttcaact atctatgaat ttgcaggcat agtctgcttc tggattttgt
     1921 tcttttgcga taaacatccc aatatatttt tttatcaaca gggacttatt atcctgattt
     1981 tagctatttt tttaatttta ccaaacagat atgtaaataa gatattccta t
//
LOCUS       DAOQWT010000313         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_64771_length_2023_cov_3.016260, whole genome shotgun sequence.
ACCESSION   DAOQWT010000313 DAOQWT010000000
VERSION     DAOQWT010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64771_length_2023_cov_3.016260"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKJ95_06265"
     CDS             <1..479
                     /locus_tag="PKJ95_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR65877.1"
                     /translation="FMGRIPFINQWQGETLLDYQISQEAMKKTDSLHHAEKGIHQISG
                     GEMQRVYIARALAQTPKILMLDEFSSHLDLSYKYEIIKILRESLKEEIQCIISVFHDL
                     NLASYCSDQLILLNQGRIEKIGTPQEVINNYYLKKVYHTKPNIIQHPELSIPQVIM"
     gene            complement(626..958)
                     /locus_tag="PKJ95_06270"
     CDS             complement(626..958)
                     /locus_tag="PKJ95_06270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015137384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNR65878.1"
                     /translation="MEKFIKGDIVIIPFPFSDLTQNKRRPALIISVVDGNDFILCQIT
                     SQNVKDNYAIQIKDNDFKTGSLKQISNIRPNRIFTADKSIILYKVASLKDKKFQEVID
                     KLIKIIRK"
     gene            complement(943..1137)
                     /locus_tag="PKJ95_06275"
     CDS             complement(943..1137)
                     /locus_tag="PKJ95_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HNR65879.1"
                     /translation="MSKKEILLKEIEQVSEPFLDEVLDFIRFLKTRNAKKRMSITVAS
                     ESSLAKDWLKPEEDEAWKNL"
     gene            complement(1373..1735)
                     /locus_tag="PKJ95_06280"
     CDS             complement(1373..1735)
                     /locus_tag="PKJ95_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65880.1"
                     /translation="MLKDRPFTCTAAHDLDRITLPIAVELSRGDERYNILNGMEKELP
                     AGDMFMEDREGITSSIIYGPDNRTQITEKTNSALYTVYAPPGIEGSLIIEHLSDIAHY
                     VQLAAPQAKMVEMVVHPL"
     gene            complement(1795..>2023)
                     /locus_tag="PKJ95_06285"
     CDS             complement(1795..>2023)
                     /locus_tag="PKJ95_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNR65881.1"
                     /translation="YMMGNDPDTGKQIKSQFEFTRLAIPRRVYTQSHLDVIIEALIAI
                     KNRVNTIPGYKINWEPKILRHFTSRLIPVDK"
BASE COUNT          634 a          358 c          294 g          737 t
ORIGIN      
        1 tctttatggg aagaatacct tttatcaatc aatggcaagg agaaaccctt ctcgattacc
       61 aaatatctca ggaggcaatg aaaaagaccg attctctaca ccatgccgaa aaaggaattc
      121 atcagattag tggtggtgaa atgcaacgag tctatatagc ccgtgccctg gcacaaactc
      181 ccaaaatact tatgctggat gaattttctt cacacctgga cttgagttat aaatatgaga
      241 ttattaaaat tctcagagaa tcattgaagg aagagattca atgtatcata tctgttttcc
      301 atgacctgaa cttagccagc tactgttcag accagctcat cttgctcaat caaggcagaa
      361 ttgaaaagat tggaacacct caggaagtaa taaataatta ttatctaaaa aaagtttatc
      421 acactaaacc caacattatt caacacccgg aactttccat cccccaggtc atcatgtaat
      481 tttgtaaagt ctatttatct gcaaagtttt atcatcattt ttcgattaat gacattttat
      541 tttttgattg aagtatagta ttaatggctt tcagaatatt tttttaaata atagtcaact
      601 cattggtatc gtctcttttt attaactact tccttataat tttaattaac ttatctataa
      661 cttcttgaaa tttcttatct tttaggctgg caactttata taagataatg gatttatctg
      721 cagtaaatat tctatttggt ctaatattgc taatctgttt taaacttcct gttttaaaat
      781 cattatcttt aatttgtatg gcataattat ccttaacatt ttgacttgtt atttgacata
      841 ggataaaatc atttccgtct acaacagaaa taatcaatgc tggtcgtctt ttattttgag
      901 tcagatcaga aaatggaaaa gggattatta ctatatcacc ttttataaat ttttccatgc
      961 ttcatcttct tctggtttta gccaatcttt ggcaagtgaa gattcactcg caacagtaat
     1021 actcattcgt tttttggcat ttcttgtctt taaaaatctg ataaaatcta agacttcatc
     1081 gaggaaaggt tcagaaactt gttctatttc ctttagaagt atttcctttt tgctcatatt
     1141 aaaacctact atcttataca aaaaataaat tgtgtatcta aattatagca tactactagc
     1201 tacaattgta tttgtaatta taatttatta tatctgaact aagttataat gacatttatg
     1261 ccaacaataa cttatgcagt tagcttcagg caatatctat gcatctattt atttaaaaat
     1321 aaattaagaa tatgacaaat tattaaaaat aaataatttg taaaaatgag tattacaacg
     1381 gatggactac catttccacc atttttgctt gaggtgctgc cagctgtaca taatgggcaa
     1441 tatcactcag atgttcaatt attagagacc cttctattcc tggcggagca tatactgtgt
     1501 aaagcgcact gttggttttc tcagttattt gagttcgatt atcaggtcca taaattatgc
     1561 tggaagtgat accttcccta tcttccataa acatatcccc ggcaggtaat tccttttcca
     1621 taccatttaa tatgttatat ctttcatctc ctctggacag ctctactgca ataggcaagg
     1681 taattctatc cagatcatgt gccgcagtgc atgtaaaggg acggtccttt aacatgtttt
     1741 ttattctgca aactaaagtt taactaatac gacttcttta cttttacctt acgtttattt
     1801 atctacaggt attaatctgg aggtaaaatg gcgtaagatt ttaggttccc agtttatctt
     1861 ataccccgga attgtattta ccctgttttt aatagcgata agggcttcta ttatcacatc
     1921 taaatgtgat tgggtatata ctcttctggg aatagccaat cttgtaaatt caaattgtga
     1981 tttaatctgt ttgccggtat ccggatcatt ccccatcata taa
//
LOCUS       DAOQWT010000314         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_64798_length_2023_cov_2.274390, whole genome shotgun sequence.
ACCESSION   DAOQWT010000314 DAOQWT010000000
VERSION     DAOQWT010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64798_length_2023_cov_2.274390"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..419)
                     /locus_tag="PKJ95_06290"
                     /pseudo
     CDS             complement(186..419)
                     /locus_tag="PKJ95_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            complement(576..2023)
                     /gene="dnaK"
                     /locus_tag="PKJ95_06295"
                     /pseudo
     CDS             complement(576..2023)
                     /gene="dnaK"
                     /locus_tag="PKJ95_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008807.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
BASE COUNT          532 a          444 c          298 g          749 t
ORIGIN      
        1 tgcaatcagt gtaattgcaa ggaacttctt catttatttc ctcctacatt tccaaagaga
       61 tctccaaaat caaactctac tcaagagttt ctccctcctc ctcaaaatgg ggaaaagaga
      121 tcttcaaagc ctcaaaatca tcctgaactt gctcctcaaa atggatttcc tccaccagat
      181 cagccaaaag gatttccaga gctacttcca aacatatcat actgtttgcg ttttccctca
      241 tcgctgagag tttgataggc ttcatttatc tccttaaact ttttttctgc ctcttcctta
      301 ttattggggt tagcatcggg atggtattgc aaagccagct tgcggaatgc tttttttatc
      361 tcatcctgac tggcatcttt atttattccc agtatctgat aataatcctt attagccaaa
      421 gttatacaac ctctttaaaa aatataagtc tataataagt ttatcttgct gaatttccgg
      481 ataatttaat atagtatatt atcttaccca gaaagtcagc aagattataa agtaattata
      541 atagttttaa tactattctt tatgtttgac aaatactatt tagaattgtt tccatcttct
      601 tctttgtatt cagcatcaaa taccttctca tctttatttg catcctgtgt atcttctgtt
      661 tgctgctcag aagaagaagt gtcagaataa gtatgttgtt cttgttgtgc tgaagattgt
      721 ttgtaaatct cttcagctaa tttatgcgaa gaattagtta gtttttcaat gccggctttc
      781 atctcttcca cattttcttt ctcaattgct tttttcaatt catttatatt ttcatttatt
      841 tcatttttca ggttctcgct aatcttgcct tccgcttctt taagagtttt ctcagtagaa
      901 taaaccagat tatctgcctg attatgtatt tccactttct ctttttgctt taagtcttca
      961 tcggcaaatt gttccgcttc tctaattttt ttctgaattt cttcttcagt aagaccactg
     1021 gaatgcttaa tggtaatctt ttgttctttt ccagtacctt tatctttagc agttacttta
     1081 aggataccat tggcatcaat atcaaaagct acttctatct atggcatccc tctgggagca
     1141 gctggaattc ctaccagctg gaaacgtccc aggctggtat tatctttagc taaagatctt
     1201 tccccctgca atacatttat atcgaccgca gtctggttat ctgctgcagt ggaaaatatc
     1261 tgatttctgg atgccggtat agtagtattt ctatcaatta atttagtaaa tacaccccct
     1321 aaggtctcta tacccaaaga taatggcgtt acgtccaata attggatatc tttgacttct
     1381 ccttttagtt ttccaccctg gatagcagca cccatagcta ccacctcatc gggattaact
     1441 cctttatgag gttctttgcc aaatatttgt ttcactattt catgtacttt aggcattctt
     1501 gtttgaccgc ctactaaaat aacttcatca atatcatttg cagtcaattt tgcatcttcc
     1561 aatgctttct tacagggctc catagtcctg gccaggatat cttcggttaa ttgctccaat
     1621 ttagccctgg ttaaagtata attgaggtgc tttggtccag tggcatctgc tgtgataaag
     1681 ggcagattaa tatcagtttc cagagaagaa gaaagctcac attttgcctt ctctgctgcc
     1741 tcttttaatc tctgtaaagc catctggtct ttacctaagt caataccctg ttccttctta
     1801 aaattatcta tcaaccagtt gactactcgc tgatctaagt catctcctcc caggaaagta
     1861 tctccgttgg ttgacttgac ctcaaaaaca ccctctccaa tatctaaaat agagatatca
     1921 aaagtacccc cgcctaagtc aaatacggcg attttttcat tcttttcctt atctaaccca
     1981 taagctaaag cagctgcggt aggttcattt attattctca aga
//
LOCUS       DAOQWT010000315         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_65064_length_2017_cov_3.542304, whole genome shotgun sequence.
ACCESSION   DAOQWT010000315 DAOQWT010000000
VERSION     DAOQWT010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65064_length_2017_cov_3.542304"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..>2017)
                     /locus_tag="PKJ95_06300"
     CDS             complement(242..>2017)
                     /locus_tag="PKJ95_06300"
                     /inference="COORDINATES: protein motif:HMM:NF017826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HNR65882.1"
                     /translation="AKELKIDQELIRQRIEQRFQELNKKQDTKPLKNYIPTIPALEDF
                     MIPTGWIVNLNGLTKLSLNKDGLKETLVATAPFYASSRSINLDSAQEKLELTYLRDES
                     WKTIYEERATLLDHRKVVQLAGKGFPVNSLNCKEIVNFINNFEALNDKILPKKTTISG
                     FGWKKGKGSPFFVMPEKAYGTNRSIVFEPESPGDERLARALRKEGNLNNWLRGIRRCI
                     DYPRVMFHIYASLTSPLLEIIDAPNFIIDNWGLTSIGKTTVLEIAASVWGLPIKELGG
                     LIIAWDATKVSIERLACLFNFLPILLDDSQTANEKIIAMILYMLANGVGRFRGALKGI
                     QKTSNWRTVAFSTGERRLSEVTEYEGAQARIIELYGSPFDTENKSELIYKIKEDIHAN
                     YGLAGPLFIEELLNKGYHESNKVLREEYRQATSELAKLGKNNVSDRMAQYFASIQVAA
                     RIAEDVFHFGGESQKIVRKMFEYTCGENKERGDYPQRALEHIISWVQGNQNSFDRQDQ
                     LATVERGEIFGVIREGEYIGIFPHKFKEIIKRSEFDSSSIIKAFKEKNWIQATKGKHT
                     YQIGFRSKVVRMIKLQWDVIKDLWY"
BASE COUNT          545 a          363 c          335 g          774 t
ORIGIN      
        1 ataggggtta aagcaggggt taagcagggg gttaaaaggg gttaaggggg gttaaggggg
       61 ttaagcaggg gttaagcagg gggttaagat agtattactt atattatata tataattata
      121 tttatattat tatatttaac cccttaaccc cttataattt ttttcttatt agataaaata
      181 gaaaaaatat taagatagga ttttacacaa aatttatgac actctcttaa tatataaatt
      241 attaatacca tagatctttt atcacatccc attgtagttt tatcattcta actactttac
      301 ttctaaaacc aatttgataa gtatgttttc cttttgtagc ttgtatccaa tttttttcct
      361 taaatgcttt aataatagaa gaactatcaa attcgctacg tttgattatt tctttaaatt
      421 tatgaggaaa gatccctatg tattctcctt ctcttataac tccaaatatt tctcctcttt
      481 ctactgttgc cagttggtct tgcctgtcaa aactattttg attcccctga acccatgaaa
      541 tgatatgttc taaagctctc tgcgggtaat ctcctctttc tttattttct ccacaagtgt
      601 attcaaacat ttttcttaca attttttgcg attctccacc aaaatgaaaa acatcttctg
      661 ctattcttgc tgctacctgg atagatgcaa aatattgtgc catacgatct gaaacattgt
      721 tttttcctaa ctttgctaat tcagatgtgg cctgtctgta ttcttctctt aagaccttat
      781 ttgattcatg atatcctttg tttaatagtt cttcaataaa aagtggtccc gcaaggccat
      841 agttggcatg aatatcttct tttattttat agatgagttc tgatttattt tcagtgtcaa
      901 atggtgagcc gtagagctct attattcttg cctgtgctcc ttcatattct gttacttcgc
      961 tcagtcttct ttcacctgta gagaaagcaa cagttctcca gtttgatgtt ttttgaatac
     1021 cttttagagc tcctctgaat cttcccacac cattggcaag catatagaga atcattgcta
     1081 taattttttc atttgcagtc tgagagtcat ccaggagtat aggcaggaaa ttgaatagac
     1141 atgcaagacg ttctattgat actttagttg catcccaggc aatgattaat cctcctaatt
     1201 cttttatagg cagtccccat actgaagcag ctatttctaa aacagttgtt tttcctatag
     1261 aggttaatcc ccagttatct attatgaagt ttggagcgtc aattatctct aatagtggag
     1321 aagtaagaga agcgtatata tgaaacatta ctctgggata atcaatacat cgtcttattc
     1381 ctctaagcca gttattaaga tttccctctt ttcttaaagc tctggcaagt ctttcatctc
     1441 cagggctttc cggctcaaat actatggatc gatttgtgcc ataggctttc tctggcatta
     1501 caaaaaaagg agagcctttt ccttttttcc acccaaatcc ggagatagtt gttttctttg
     1561 gaagtatttt gtcattgagg gcttcaaagt tattgataaa gttaactatt tctttgcagt
     1621 taagggagtt tacagggaat ccttttcctg ccagttgaac cacttttcta tgatctaaaa
     1681 gtgttgctct ctcttcatag atggttttcc aggattcatc tcttaagtac gttaattcga
     1741 gtttttcctg tgcagaatcc aggtttatag atcttgaaga tgcataaaaa ggtgctgttg
     1801 ctactaatgt ttctttcagt ccgtctttat tgagagagag ttttgttaat ccgtttaggt
     1861 taactatcca gccggtagga atcataaaat cttccagtgc cggtatagtc ggtatataat
     1921 tttttaatgg ttttgtatcc tgttttttat ttaattcctg aaatctctgt tctatcctct
     1981 ggcggataag ttcctgatct atttttaatt ctttggc
//
LOCUS       DAOQWT010000316         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_65427_length_2010_cov_5.495141, whole genome shotgun sequence.
ACCESSION   DAOQWT010000316 DAOQWT010000000
VERSION     DAOQWT010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65427_length_2010_cov_5.495141"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PKJ95_06305"
     CDS             complement(<1..1319)
                     /locus_tag="PKJ95_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65883.1"
                     /translation="MKSNIRVTRIVKDEKGFTAFSFGGFYCGQFGITTVIKFEAGTGV
                     KACFNFKFFSTEILGALREFPTQKEQNLRFYLQERKTGINAAVSQAVRRINKDLPYEK
                     KFFYEAYKIDNRIDSINFWGEISYLVFSNLTKNGEATYTIFCEILREDITKLEEKVYN
                     ALLNEITKELKEDGIEQEITEKLEMIANSEYAIEDYTDWKKFYVIDNSLSEQELRKNY
                     LLYITETIEEYMPELLQETGKDIEIIDIEKKQVPLDLQMFASAVSGLVLSPEDLKERK
                     EKREKDKAQQQVINICQSLNWNIDEHGGFAGHGKYGTSTYNRGDYFSISILDILPYKK
                     SYRKPYFNMSKDHLPLSLVRLNRKRVYARSSNWFPHTTSSLYLVGQNESGTYFAHAVS
                     SRLQASDKRRNNPTLLDALLWIWNCPLDLLLRRQGDIAVVKSSGRSHV"
     gene            1614..1829
                     /locus_tag="PKJ95_06310"
     CDS             1614..1829
                     /locus_tag="PKJ95_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65884.1"
                     /translation="MAIYRKIDQERAWRSIKKRALERFKSGIDVFIDSASENSIEAKI
                     EASIYISVDMWQVWEDRGKKEDLWKKD"
BASE COUNT          621 a          350 c          340 g          699 t
ORIGIN      
        1 acatgagacc tgccggatga cttcacgacg gctatatctc cctgccgacg aagaagaaga
       61 tcaagaggac aattccagat ccagagaagc gcgtcaagca atgtcggatt gttgcgcctt
      121 ttgtcagaag cctgaagcct tgaagaaact gcatgggcga aatatgtgcc gctttcgttc
      181 tgtcctacca gatacaaact tgaagtggtg tggggaaacc aattcgaaga acgagcataa
      241 accctttttc tattgagtct cacaagactg agtggaagat gatccttgct catattgaag
      301 tatggttttc tatagctctt tttgtacggg agaatgtcca gtattgaaat actgaaataa
      361 tctcctctgt tataagtcga tgtaccatat tttccatgtc ctgcaaagcc gccatgctca
      421 tctatattcc agtttaagga ctgacagata tttattacct gctgttgtgc cttatctttc
      481 tctcttttct ccttcctctc tttcagatct tcaggagaaa gcaccagacc ggaaacggca
      541 gaggcgaaca tttgaagatc aagagggacc tgttttttct ctatatcaat gatctctata
      601 tcctttccag tctcctgtag taattccggc atatattctt ctattgtctc agttatataa
      661 agaagatagt ttttccttaa ttcctgctca gataatgaat tatcaattac atagaacttt
      721 ttccaatctg tgtaatcttc gattgcatat tcagaatttg caatcatttc taatttttct
      781 gttatttcct gttcaatacc atcttcttta agttcttttg ttatctcatt taaaagggca
      841 ttatatacct tttcttctaa cttcgttata tcctctctga gaatttcaca gaaaattgta
      901 tatgttgctt caccattttt tgtgagattt gagaaaacaa gataagaaat ttcaccccaa
      961 aaatttatac tatctattct gttatctatt ttataagctt cataaaaaaa cttcttttca
     1021 tatggaagat ctttgtttat ccttctgact gcctgactga ctgcagcatt tattccggtt
     1081 tttctttcct gcaggtaaaa tcttaaattt tgttcttttt gtgtcgggaa ttcccgtaaa
     1141 gcgcctaata tctcagtaga gaagaattta aagttaaaac aagctttaac tcccgtgcct
     1201 gcttcaaatt taataacagt cgtaatgcca aactggccac agtagaaacc gccaaaacta
     1261 aaagcagtaa accccttttc atcttttact atccttgtta ctcttatgtt acttttcatc
     1321 ttcctttacc ttcctttttt attatttgtg aactccctat gttcaccttc cccactactt
     1381 attatacaca tatttaaaac aatgtaaata ccctattttt tggcgttatt actggcttta
     1441 tttaaatatt gattttatga cagtttgatt taagaataag gaaatcctta tacagtaaag
     1501 gaaagagtga tttatatgag tgagtggaat atagagaaat atttttatat atttttttaa
     1561 aaagaaaaaa cataaggata acgccattct ctatgatagt gatattctga tatatggcta
     1621 tatataggaa gatagatcag gaaagagcat ggaggagtat caaaaagagg gctttagaga
     1681 ggttcaagag tgggatagat gtatttatcg attctgcatc agagaatagc atagaagcga
     1741 aaatagaagc atctatatat atctctgtcg atatgtggca ggtctgggag gacagaggaa
     1801 agaaggaaga tctctggaag aaggattgaa tgtgatgtaa tgtaatcact tcttacatta
     1861 tgtaatcatg attagttaga gtgaattatt ttatcataat gcttagtata tatgaattat
     1921 ttagtatata tagaaataat aagcatatat actttataaa gtgattacat aacatcaaaa
     1981 gtgattacat gattacatta gtgtaaataa
//
LOCUS       DAOQWT010000317         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_65867_length_2002_cov_3.935799, whole genome shotgun sequence.
ACCESSION   DAOQWT010000317 DAOQWT010000000
VERSION     DAOQWT010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65867_length_2002_cov_3.935799"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKJ95_06315"
     CDS             <1..505
                     /locus_tag="PKJ95_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNR65885.1"
                     /translation="SETNTGSKSIAEEEICIIHISGAVNQPGVYQLSKGKRIIDAVKM
                     AGEATEKANLDAVNLAAPVYDGQKIIIPYLITAEGMDNELQMKGNSGIDYSTNFSFKD
                     SSSININNCSARELESLEGIGPVLAERIVEYRRNNGPFGKIEDIRKVPGIGEKRFEAI
                     KEMITVY"
     gene            549..1970
                     /locus_tag="PKJ95_06320"
     CDS             549..1970
                     /locus_tag="PKJ95_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNR65886.1"
                     /translation="MLISYIIGIIIQRNCIILTTKSLFLFCLIVLFLSFISYGRRWNL
                     TGVFLLLCFLLFGMLNTSFQSQPLGSNHIYQFVSKPGILTGRICEAKFDSNHQYLEIL
                     LSVQSFKEYDQHYAVQGKVLLIINGDGSSLQYGDLIEAKVVLQEPPLPNNFGEFNYRE
                     YLARQKIFVTDRINIDQVVLITSKRQIGLPYLKNSLRNKIENKIDILYRYPYNALIKA
                     VTTGNRKEIPLEWELIFQDAGTMHILAISGLHVGIIASVLFFIFKLIPYLGRRKNLSY
                     TLIIVFLLGYAAIAGFRPSVNRATLMFITMLGARYFNRPYHLSNSLYWTAFLLLFYQP
                     LLLFDAGFLLSFTVTFFIIFLYPIIKEKLNFLPLCISQPLAVSTAAWLGMAPLSAYFF
                     YKVSFIALLSNLIIVPLISVILVLGIISIGFSVIYLPFAGLFVLLNQIFINFLIQITK
                     VFTSLPFAAIYLAQPKIYQIIYY"
BASE COUNT          609 a          352 c          348 g          693 t
ORIGIN      
        1 cagtgagacc aatactggca gtaaaagtat tgcggaagag gaaatatgta ttatacatat
       61 ttccggagcg gtaaaccagc cgggtgttta ccagctgtct aagggtaagc gcataattga
      121 tgccgtaaag atggcaggcg aagctacaga aaaagctaat ctcgatgctg taaatctggc
      181 ggcacctgta tacgatggcc aaaagataat cataccttat cttataacag ctgaaggtat
      241 ggataatgaa ttacagatga agggtaattc aggtatagat tattctacaa acttttcttt
      301 taaagatagt agttctataa atattaataa ctgttcagcc cgggaattag aatctttgga
      361 gggtatagga cctgttttag cagaacgtat tgtggaatat cgtagaaata atggcccttt
      421 tggcaagata gaagatatca gaaaagtccc gggaatcggt gaaaaaagat ttgaagcaat
      481 taaggagatg ataactgtct actaagcaga tatatccatt atccaatttt caggtaccgc
      541 tgctatcttt gctgatatcc tatattattg gaataataat tcaaagaaat tgtattattc
      601 tgactaccaa atcccttttt ttattctgcc ttatagtcct gtttttaagt tttatctcct
      661 atggcaggcg ctggaattta acgggtgtat tcttgctact ctgttttcta ttattcggaa
      721 tgctgaacac ctcctttcaa agccaaccac taggctctaa tcatatttat cagtttgtaa
      781 gcaaaccggg aattttgact ggtagaattt gtgaggcaaa attcgattca aaccatcagt
      841 atctggaaat attactttct gtccaatcct ttaaagaata tgaccagcat tatgctgtac
      901 aaggcaaagt cctgcttata atcaatgggg atgggtcttc tttgcaatac ggagatctga
      961 ttgaggcaaa agtagtatta caagaacctc ccttacctaa taattttggc gagtttaatt
     1021 accgggaata tttagccagg cagaaaatat ttgtaacaga ccgtattaat attgatcagg
     1081 tagttctgat taccagtaag agacaaatag gcctacctta tttaaaaaat tccttgagaa
     1141 ataaaataga aaataaaatt gatattctct accgctatcc ctataatgcg ctcataaaag
     1201 ctgttactac aggcaacaga aaagaaattc ctttagaatg ggaactaatt tttcaagatg
     1261 ccgggactat gcacatactg gcaatttcag ggctacatgt tgggataatt gcctcagtac
     1321 ttttctttat tttcaaatta attccctatt taggcagaag aaaaaattta agttatactc
     1381 ttattattgt tttcctgctg ggctatgctg ccatagccgg tttccgtccc tcagtcaaca
     1441 gagctacttt gatgtttatt actatgcttg gtgccagata ttttaacagg ccataccacc
     1501 tttctaatag tctttattgg acagcatttt tattactatt ttatcaacct ttgttattat
     1561 ttgatgccgg ttttttgctt tcttttactg taactttttt cattattttt ctctacccga
     1621 taataaaaga aaaattaaat ttcctgcccc tttgtatttc tcagccattg gcagtttcca
     1681 cagctgcctg gttgggaatg gctccgctat ctgcctattt cttctataag gtctctttta
     1741 ttgctctttt atcaaatttg attatagtcc ctttaatcag tgtaatttta gttttaggaa
     1801 taatctcaat tggcttttca gtaatatatc ttccttttgc aggattattt gttttactaa
     1861 atcaaatatt tatcaatttt ttaatacaga ttactaaagt attcacttct ttaccatttg
     1921 ccgctatata tctggctcaa ccaaaaatat atcaaattat ttattattaa gagtatctgc
     1981 atccaaggat gcggctttaa at
//
LOCUS       DAOQWT010000318         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_65890_length_2002_cov_2.589625, whole genome shotgun sequence.
ACCESSION   DAOQWT010000318 DAOQWT010000000
VERSION     DAOQWT010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65890_length_2002_cov_2.589625"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /gene="purE"
                     /locus_tag="PKJ95_06325"
     CDS             complement(<1..438)
                     /gene="purE"
                     /locus_tag="PKJ95_06325"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNR65887.1"
                     /translation="MEVSIIMGSISDQPIMEKATEVLQELDIGYEVRAISAHRSIDFL
                     FHYIGQFSQRGFRVIIAGAGGAAHLPGVIASKTLLPVIGVPIKTATLEGLDSLLSIVQ
                     MPKGVPVATVGINNAANAALLAARILALQDDGISKKLASYIKEM"
     regulatory      complement(645..748)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(868..1395)
                     /locus_tag="PKJ95_06330"
     CDS             complement(868..1395)
                     /locus_tag="PKJ95_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNR65888.1"
                     /translation="MESLFEIFISFLIIGIGAYGGGLVTIPLIQHEIVVKYNWLTLRE
                     MSELLAIAQMTPGPISINTATFTGYRLAGVAGSIIATAAVVLPSIIFFTLLTPIITQF
                     KENSHFYRINKSLQLGVLSLILFAVWSYGSAVIRGYLDFGIATAAFLLLVNCEKKLHP
                     LIIILVCGIIGLIFF"
     gene            complement(1453..>2002)
                     /locus_tag="PKJ95_06335"
     CDS             complement(1453..>2002)
                     /locus_tag="PKJ95_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNR65889.1"
                     /translation="KLNHKEKNILLIDIFLTFFKVGLLTLGGGLAMSPVMRYELVLKR
                     KWLSESEFSSTVALATIVPGSISVNIAYLLGKKLRGWKGLTLSVLGVVLPSFITILLI
                     ASLLLPFFHYPKVVNFLRGCAIAVVGQLAFASLIFGKRLIRRISHIVICAVGILMMGV
                     LKLHPIFGLVVVSLIGYWFYPG"
BASE COUNT          705 a          400 c          318 g          579 t
ORIGIN      
        1 catttcctta atataagatg ctaatttttt tgagattcca tcatcttgca gagccaaaat
       61 tctggctgcc aagagggcag cattagccgc attattaata cctactgtgg caacaggtac
      121 ccctttaggc atttgtacaa tagacaacaa agaatctaat ccttctaaag ttgctgtttt
      181 tataggtact ccgataacag gtaataaagt tttactggca ataacgccag gaagatgtgc
      241 cgcacctcca gctccagcaa taataaccct aaaacccctc tgagagaact gcccgatata
      301 atgaaaaaga aaatctatag aacggtgggc tgaaatagcc ctaacttcat aaccaatatc
      361 taattcctgc aggacttccg ttgccttttc cataataggc tgatcagaaa tactgcccat
      421 aataatactt acctccattt agaacctcct ttaaaaataa tttaacctaa gattacttct
      481 ataaaaaaat aaaaacccag tagaggagta ttccaaaaaa gagtggaacc tctccgcctg
      541 ggattttagc cctacggtgt agtataaata tacactttgc accacttggt tcaaaccctg
      601 ctttataatg ctattcaggc tatataaatc tggcaggtga atccctaaga tgcacttcca
      661 ctcgtagccc attgtgattt acggtcactc ggtagagacg cttccgccct attcgaaagc
      721 ttatacgagt ttttaaatta aaaaaatcta atgaaaatat accattctta tctataattg
      781 tcaaacagat aaaaattata ctattagata ggactgaata gaagtttgag gtcaatactg
      841 aatattatga taaatctata gctaatttta aaagaatatt aggcctataa taccgcaaac
      901 taaaatgatt atgagtgggt gtaatttttt ttcacaatta actaaaagta aaaaagcagc
      961 tgttgcaatt ccaaaatcta agtatcctct gataactgct gagccatagc tccagacagc
     1021 aaaaagaata aggctcaata cacctaattg taaactttta tttattctgt aaaagtggga
     1081 attctcttta aattgagtaa ttatgggggt taacagggta aaaaatatta ttgaaggcag
     1141 tacaacggct gctgtagcta ttattgaacc agccactcct gccagccggt aaccggtaaa
     1201 agttgcagta ttgatagata ttggccctgg tgtcatctgg gcaattgcaa gtaattcaga
     1261 catttccctg agggtcaacc aattatattt aacaacaatt tcatgctgaa tcaaagggat
     1321 tgtaaccagc cctcctccat aagcaccaat tcctattatt aaaaaactga taaatatttc
     1381 aaataaagac tccaattact taatccccaa tacaaaattt tttctacaac ttttttaata
     1441 ttaaaaatac ctttaaccgg gatagaacca ataaccaatc aggcttacca ctactaatcc
     1501 gaatatgggg tgaagtttta atactcccat catcagaatt cctacagcac atatcacaat
     1561 gtgtgagatg cgacgaatca gtcttttacc aaaaatcaag ctggcaaagg ccagctgacc
     1621 aacaactgca atagcacaac cacgtaaaaa gtttacaact tttggataat gaaaaaatgg
     1681 gagaaggagg cttgctataa gaagtatggt gataaaagag ggtaagacca ctcctaatac
     1741 cgatagagtt aagcctttcc atcctcttaa ttttttccct aacaagtaag caatgtttac
     1801 tgagatagag cctggaacaa tcgttgccag ggcaacggta gaagaaaatt ctgattcact
     1861 cagccatttt cttttcagaa caagctcata cctcataacc ggtgacattg ccagacctcc
     1921 gcccaatgtt aataaaccta ctttaaaaaa agttaaaaaa atatctatta ataaaatatt
     1981 tttttcttta tgatttaatt ta
//
LOCUS       DAOQWT010000319         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_66298_length_1994_cov_3.273853, whole genome shotgun sequence.
ACCESSION   DAOQWT010000319 DAOQWT010000000
VERSION     DAOQWT010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66298_length_1994_cov_3.273853"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PKJ95_06340"
     CDS             <1..592
                     /locus_tag="PKJ95_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNR65890.1"
                     /translation="MPFRNNIQNHLHKIREIDIDIIAPSHGPIYKNPDFILKNYQEWV
                     SDSVKDEVVVPYISMHGSTEAMVKYLVEQLIKRGKKVTPYHLTNTDIGELAMALVDAA
                     TVILASPTVLTGPHPQIVYAAYLCKLLRPKTRFAGIIGSFGWGSKMEQDILQIIGNLK
                     VELLPTVIARSHPRAEDFKKLDLLVEQIILKHQTLK"
     gene            625..1146
                     /locus_tag="PKJ95_06345"
     CDS             625..1146
                     /locus_tag="PKJ95_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65891.1"
                     /translation="MKKKFLAFLLFLFIFVTFGSAINSQESLDEILYRDFKDVETFGY
                     INVKVQGEQTFSIGLTSEELTDYARLKYKNNFAAIPFQEIIAGEAYLYQEEEKAKKVG
                     SIWFRVWTVGEEFPIAYYIECRAGTYKNYEMWKDEVLGFCDKREIKQIVRHEITRMME
                     NLAITFFKVRGEI"
     gene            1271..>1994
                     /locus_tag="PKJ95_06350"
     CDS             1271..>1994
                     /locus_tag="PKJ95_06350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR65892.1"
                     /translation="MNIKNLFEDYTKLPRSMFILFFAQIVSSAGNFVFPFLAIFFTKT
                     LIFNEQKAGFYVMMATAAKVPGLLLGGKLSDVFGRKKIFIIFSTLSALFVFFCLLVGQ
                     IGQNEIIPWLLVLSAVFNGANYPAMKAMVADLTNPDNRKAAFSLLYLGMNIGFAIGPL
                     IAGLLYNNYLKLLFVGDTATTLISVALVYFLISETLPGPNNLGKSSTTEYNSERAEEG
                     SIYRVLLSRPYLIIFTFIFTLYS"
BASE COUNT          629 a          313 c          409 g          643 t
ORIGIN      
        1 gatgcctttt cggaacaata ttcagaatca tctccacaag attagagaga ttgatattga
       61 tattattgcc cccagccatg gaccaattta taaaaatcct gattttattt taaaaaatta
      121 tcaggaatgg gtatcggatt cagtcaaaga tgaggtggtt gttccctata tttctatgca
      181 tggtagcact gaagctatgg tgaaatattt agtggagcaa ttaattaaga gaggaaaaaa
      241 ggtaactccc taccatttga ctaataccga tattggtgag ttggcaatgg cactggttga
      301 tgcggcgaca gtaatcttag cttcccctac agtattgact ggaccacatc ctcagattgt
      361 ttatgctgcc tatctctgta aacttttaag accaaaaaca cgctttgcag gaattatagg
      421 ttcatttggt tggggaagca aaatggaaca ggatatattg cagataattg gtaacttaaa
      481 ggtcgagtta ttgccaacgg ttattgccag gagtcatccc cgggctgagg attttaaaaa
      541 attagattta ttggttgaac aaattatatt aaaacatcag accttaaaat agatgagtac
      601 atatagagta ggggtgaaca aaatatgaaa aagaaatttt tagcattttt gctattcctg
      661 tttatctttg ttaccttcgg cagtgctata aattctcagg aatcccttga tgaaatttta
      721 tatcgtgatt ttaaggatgt ggagaccttt ggctatataa atgtaaaagt gcagggggaa
      781 cagactttct caattggcct aaccagcgaa gaacttactg attatgcccg cttaaaatat
      841 aagaataatt ttgctgccat tccttttcaa gaaataattg ctggagaagc ttatctctat
      901 caagaggaag aaaaggctaa aaaagttgga agtatctggt tcagggtatg gacggtaggg
      961 gaagaatttc caatagctta ttacattgaa tgcagagcag gcacctataa aaactatgag
     1021 atgtggaaag atgaagtact gggattttgt gataagagag agattaaaca aattgtcaga
     1081 catgagataa ccaggatgat ggagaattta gccattactt ttttcaaagt gcgaggagag
     1141 atttaagtta ctgccttctt attttgaata gaatttataa taaaattgct cctgaaaaaa
     1201 atagggagga gcaatgatag aataaataaa aaataagata agtaattagg tttaattggt
     1261 gtctgactaa atgaatatta aaaatctttt tgaagattat accaaattgc cccgaagtat
     1321 gttcatatta ttttttgccc agatagtaag ctctgcgggc aattttgtct ttccttttct
     1381 ggctattttt tttactaaaa cacttatatt caacgaacag aaagctggtt tttatgtgat
     1441 gatggccacc gctgcaaaag taccgggctt gcttttggga ggaaagctgt ctgatgtatt
     1501 tggcagaaaa aaaatattta tcatctttag cactctttca gcattatttg tctttttctg
     1561 tttgctggtt ggtcaaatcg gacagaatga aataattccc tggctgttgg ttctatcggc
     1621 tgtctttaat ggtgccaatt atcccgctat gaaggcaatg gtggcggact taactaatcc
     1681 ggataataga aaagcagcct tttccctgct ctatctcggt atgaatatag gatttgccat
     1741 aggtccctta attgccggat tactatataa taattatcta aaactgctct ttgtgggaga
     1801 tacagctacc acccttattt cagttgcact ggtttatttc ttgatttcag agactttgcc
     1861 aggtcctaat aatttgggaa aatccagtac aacagagtac aatagtgaaa gggcagaaga
     1921 gggcagtata taccgggtat tgctctccag gccttatctg ataattttta cctttatttt
     1981 tactctatat tctt
//
LOCUS       DAOQWT010000320         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_66464_length_1991_cov_3.647211, whole genome shotgun sequence.
ACCESSION   DAOQWT010000320 DAOQWT010000000
VERSION     DAOQWT010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66464_length_1991_cov_3.647211"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKJ95_06355"
     CDS             complement(<1..296)
                     /locus_tag="PKJ95_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR65893.1"
                     /translation="MKDLADHLFDILENSVNAGANEVIVKIAHTKSYFFCSISDNGKG
                     INGQEVLDPFVTSRKTRRVGLGLPLLKRAVEDTCGYLKIMRIDESGGTALQFKM"
     gene            complement(268..1047)
                     /locus_tag="PKJ95_06360"
     CDS             complement(268..1047)
                     /locus_tag="PKJ95_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNR65894.1"
                     /translation="MDYCTNYWRVSIIKIDLHIHSSLSACGENIMSPQNILKKAEQSG
                     LNLIAITDHNAIAHSILISEIKKASPVNIILGVELTTKEEVHLLGYFPDEVSIREMGE
                     KINEYLPQMENRASFFGDQLIYDQEGEIVEIDCLLRQNALQISLDELVKLIHHLGGLA
                     IPAHIYRDYCSIKSQIGFLDPKSEFDAVELSRYEWIKKKCKIGDLIEGFPVISGSDSH
                     FLEDIGTFFFEVEDEHYIVDFDSLKYFLRKIKIERFSRPSF"
     gene            complement(1001..1345)
                     /locus_tag="PKJ95_06365"
     CDS             complement(1001..1345)
                     /locus_tag="PKJ95_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur binding hydrogenase"
                     /protein_id="HNR65895.1"
                     /translation="MKLNQIVDHCSLKKVVFLSDEDIKTGYCGDLMSDVIAHADNESI
                     WVTVQAHKNSIAVALIKDIKAIVFTNNVEISMELIEKAKEEKINLFSSKNNSFQICGL
                     LYQLLEGKYYQD"
     gene            complement(1348..>1991)
                     /locus_tag="PKJ95_06370"
     CDS             complement(1348..>1991)
                     /locus_tag="PKJ95_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNR65896.1"
                     /translation="QNFSLVSAIKKFEKYKFGRLPVTENSHSKKMVGILTMGDILNLL
                     LLSIQTIAEKVEEKEDRDSQLSEQLIEKSQEKPLRFEVKKADFDNAGRVASIIRTYLQ
                     RLNIDKKTTRRVAIVCYEAEMNISIHSLGGYISVTLEQNNIIITAVDYGPGIPDIDEA
                     MKPGFTTASEQIRALGFGAGMGLPNIKKCADQLELESSMETGTILKAVIRIGG"
BASE COUNT          606 a          429 c          273 g          683 t
ORIGIN      
        1 tacattttaa actgaagagc agtaccacca ctctcatcta ttctcattat ctttaaatac
       61 ccacaggtgt cctccaccgc tctttttaat aaaggaaggc ctaatcctac tcttctggtc
      121 tttctggaag tgacaaaagg gtctaatact tcctgaccgt taattccttt tccattgtca
      181 gatatagagc aaaaaaaata actcttagta tgagctattt ttacaataac ttcatttgct
      241 ccggcattca ctgaattttc taaaatatca aaaagatggt ctgctaaatc tttcaatttt
      301 tatctttctt aaaaaatatt ttaatgaatc aaaatcaact atataatgct catcttctac
      361 ttcgaaaaaa aatgttccaa tatcttctaa aaaatgacta tctgaaccgc taataaccgg
      421 aaatccttca atcaaatccc cgattttaca cttctttttt atccactcat atcttgataa
      481 ttcaacagca tcaaactcac ttttgggatc caaaaaacca atctggctct ttatactgca
      541 ataatctctg taaatatgag cagggatagc caatccacca agatgatgaa tgagttttac
      601 caactcatct aaacttattt gtagagcatt ttgtcttaac aggcaatcta tttcaactat
      661 ctccccctct tggtcataga tgagctggtc accaaaaaaa gaagccctgt tctccatttg
      721 gggtaaatac tcgtttatct tttcccccat ctctcttata cttacttcat ccgggaaata
      781 acccaataaa tgaacttctt ctttagtagt aagctcgact ccgagtatga tattaaccgg
      841 gcttgctttt ttaatttcac tgattaagat agaatgtgca atcgcgttgt gatcagtaat
      901 ggcaattaga tttaatccac tctgttctgc ctttttcaat atattttgtg gagacataat
      961 attttcccca caggcagaaa gagaagaatg gatatgtaaa tcaatcttga taatacttac
     1021 cctccaataa ttggtacaat aatccacata tctgaaaaga gttattctta ctgctgaaca
     1081 agttgatttt ttcttctttt gctttttcta tcagttccat acttatttct acattattgg
     1141 taaagacaat ggccttgata tcttttatta atgctactgc aatactgttt ttatgggctt
     1201 gaactgtgac ccatatgctc tcattatcag catgagcaat aacatctgac atcaaatcac
     1261 cacaataacc agtctttata tcctcatctg aaagaaatac aactttcttt agtgaacaat
     1321 ggtctacgat ctgatttaat ttcatcttta tcctcctata cggatgactg cttttaaaat
     1381 ggtgccggtt tccatcgaag attctaattc cagctgatca gcacattttt tgatatttgg
     1441 caatcccata cctgccccaa accccaaagc ccttatctgt tcagaggcgg ttgtaaatcc
     1501 cggcttcata gcctcatcta tatcagggat acctggccca tagtcaacag cagtaattat
     1561 aatattgttc tgttccagtg ttactgaaat ataccctccc aatgaatgga tgctgatatt
     1621 catctctgcc tcataacaaa caatggccac tctgcgggta gtctttttgt ctatattcaa
     1681 tctttgcaga tatgttctta taatactggc tacccttccg gcattatcga aatcagcttt
     1741 tttaacttca aaacgaagtg gcttctcctg agacttttca attaactgtt cagaaagctg
     1801 agaatctcta tcttcctttt cttcaacttt ctcagcaata gtttgaatac tcaagagcaa
     1861 cagatttaag atatcaccca tagtcaaaat tccaaccatt tttttagaat gggaattctc
     1921 tgttactggc aatcggccaa atttatattt ttcaaacttc ttgatagcag aaacaagaga
     1981 aaaattttga g
//
LOCUS       DAOQWT010000321         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_66587_length_1989_cov_3.507239, whole genome shotgun sequence.
ACCESSION   DAOQWT010000321 DAOQWT010000000
VERSION     DAOQWT010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66587_length_1989_cov_3.507239"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKJ95_06375"
     CDS             <1..272
                     /locus_tag="PKJ95_06375"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HNR65897.1"
                     /translation="LEVARKSKEVIDICLEIARKGNKNAISDVGVGVLLAEAAFHSAI
                     INVKINLNMIKDEKIKKELAQEIESLNNQIKGKKGRVLEVVLQRL"
     gene            1028..>1989
                     /locus_tag="PKJ95_06380"
     CDS             1028..>1989
                     /locus_tag="PKJ95_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNR65898.1"
                     /translation="MSINNNKKLTQILSTLPDMYKILIKGKDIEKVRKKLFNFCKELE
                     WEYREGLRTLHKIDFGITLDAIKVFANLISKRNEKMVGFSTLQYLWELANNEIKDCQQ
                     VSESFLEECRHLFLAMAGKSNIAHGWLGPILEKEGINMIDFQMVKGREAGKARSNYLD
                     HLYNKVNEYISRYPSGLDDTLIQERKKNIFKIMDFFSANIEDWYDYQWQIKHIFKDKE
                     GLEQLKQLIPLADVDIQAIELSLEQSVPFGITPYYLSLFDFTRSDRKYDYQVRSQVIP
                     PLHYVELMGKHRQERNYYFDFMGEHDTSPEELITRRYPMVSILKA"
BASE COUNT          762 a          272 c          332 g          623 t
ORIGIN      
        1 cattagaagt tgcacgaaaa agtaaagaag taatagatat ttgtctggaa atagcccgta
       61 aggggaataa gaatgccatc agtgatgttg gagtaggtgt attactggca gaggctgctt
      121 tccatagcgc tataatcaat gtaaaaatta atctaaatat gattaaggat gaaaaaataa
      181 agaaagaact ggctcaggaa atagaaagtc ttaataatca aataaaaggt aaaaagggta
      241 gagttttaga agttgtattg caaagacttt aaatgttttt aagaaaagaa ttaaaaataa
      301 taaatatacc aattttaagt tcaatcgcta ctcctgaaac aaattaataa aaatactcct
      361 tccagtgata tgaatgtgct ggcattgaac ttgatcaaga tttaatccac ctttttaaat
      421 attatggtat aatgctaaca cattagaatc tataaataaa ggtaaatcac tttaattatc
      481 aagtaataat tctgtattta atacaatcag gaacatataa tttaaatttg gaattaaaat
      541 ttatttacaa ttaaatactg taaataaatt ttaaagataa acattagatt agattagagt
      601 taattgccta ttatttatta aaaactacga gaaaaccaca tctttaactg ctgtagggaa
      661 tggtaaaaga ttataatgat cgaaaaacca taaccggatt tattcttttt atagatacat
      721 atcatatctc aaactaccag aaatgttcaa aataaggtta ttcatataac tttgttagaa
      781 attactattt tcaaagttac actattttca ggaattacta caatagatgt ctttaaagaa
      841 acttaaaatt actactagca tatacagaat atctctttta ttaaataaag gctaatccta
      901 aaaggcatat ctattaaatg aaaaaatctt atctaaatgt ccggaatagg atggagcaag
      961 caatgttact aaagattatc aataactgga ttgattgtga gagatgtttt taataaaggg
     1021 gactcctatg agtataaaca ataataaaaa actaacacaa atattatcta cactaccaga
     1081 tatgtataaa atattaatta agggtaagga tattgagaaa gtcagaaaaa aactatttaa
     1141 tttctgcaaa gaattggaat gggaataccg agaggggcta agaacactcc ataaaattga
     1201 tttcggtatt accttagatg ctattaaggt atttgctaat cttatttcta aacgtaatga
     1261 aaaaatggtg ggtttcagca ctctgcagta tctatgggaa ttagctaaca atgagattaa
     1321 agattgtcaa caagtttccg aatctttttt agaagaatgc cgacatctct ttttagctat
     1381 ggcaggaaaa tcaaatatag cacatggctg gctgggtcct attttagaaa aagaggggat
     1441 taatatgatt gactttcaga tggtaaaagg aagagaagct ggcaaggccc gctcaaatta
     1501 cctcgaccat ctttataata aagtcaatga atatatctcc cgttatcctt caggtttaga
     1561 tgatacatta attcaagaac gaaaaaagaa tatatttaaa atcatggatt tcttttcagc
     1621 caatatagag gattggtatg attaccagtg gcagataaaa catattttta aagataaaga
     1681 aggattagag cagttaaaac aactaatacc gttagcagat gtggatattc aggctattga
     1741 actatcttta gagcaaagcg ttccctttgg cattacccca tattatcttt ctctcttcga
     1801 ttttactcgt agtgatagaa agtatgatta ccaggttcgt tctcaggtaa ttccgccttt
     1861 gcattatgtt gagcttatgg gtaaacatcg gcaggagaga aattactatt ttgactttat
     1921 gggtgagcat gatacctctc cagaagaact aattacccga cgctatccca tggtctctat
     1981 attaaaagc
//
LOCUS       DAOQWT010000322         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_66883_length_1984_cov_2.263349, whole genome shotgun sequence.
ACCESSION   DAOQWT010000322 DAOQWT010000000
VERSION     DAOQWT010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66883_length_1984_cov_2.263349"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="PKJ95_06385"
     CDS             <1..1302
                     /locus_tag="PKJ95_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR65899.1"
                     /translation="KVLNLTDKVARTERHHAHIAAIKESYQTNPALHQLVRKLTKLAP
                     SYKDGMIMNFFLNAGLMGIPRQYELAKEIGVDVPWTILIDPTSACNLSCTGCWAGKYK
                     KSDSLEVETINRIITEAKELGIYFIVLSGGEPTIYPHLFDILKKHSDVGFMMYTNGTL
                     IDDVMADRMLEVGNISPAISLEGFRDSTDARRGAGTYDKIMDAMDRLHKRGIAFGISL
                     TVTRQNAGELFGTDDFIDYMVEKGALYGWSFHYIPIGKDTNLDMMITPEQRAMLAYRI
                     PEIRVKKPIFLADFWNDGTFSGGCIAGGRRYFHINAKGEVEPCAFVHFAIDNIKGKSL
                     KEVLRNPLFQSFQKRQPFSDNLLAPCPIIDNPQQLRDIVAESGAYPTHEGAETVLSGS
                     IGKYLDELSSNWHKKSRPIFEERIKKAIKVKEEYQKHRIVA"
     gene            1778..>1984
                     /locus_tag="PKJ95_06390"
     CDS             1778..>1984
                     /locus_tag="PKJ95_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR65900.1"
                     /translation="MLGCGGPIPSKPTINSFTAAPPTIAPGDSSVLSWNVTGATSVTI
                     SHGIGSVSSTGTRSTSPATTTTYLL"
BASE COUNT          626 a          379 c          386 g          593 t
ORIGIN      
        1 aaggtactta acctgactga caaagtagct cgaacagagc gacatcatgc acacatagct
       61 gctattaaag aatcctatca aactaatccg gctcttcatc agcttgttag gaaacttact
      121 aaacttgctc ccagctacaa agatggaatg attatgaact tcttcttaaa tgcaggtctt
      181 atgggtattc ccagacaata tgaattggcc aaagagatag gggtggatgt tccctggact
      241 atcttgattg acccgaccag tgcttgtaat ttaagctgta ccggttgctg ggctggaaaa
      301 tataaaaaaa gtgatagtct ggaagtggaa accattaatc gtattatcac tgaagccaaa
      361 gagctgggaa tctactttat tgtactttcc ggcggagaac caaccatata tccgcacctt
      421 tttgatatct taaaaaagca ttcagatgta ggttttatga tgtataccaa tggtacctta
      481 attgatgatg tgatggctga ccggatgctg gaggtgggta atatcagccc ggcaataagt
      541 ctggaaggat ttagggattc aacagatgcc cggcgtggag ccggtaccta tgacaagatt
      601 atggatgcta tggatagact tcataaaagg gggattgcct tcggtatcag tctcactgta
      661 accagacaga atgcagggga actttttggg acagatgact tcattgacta tatggttgag
      721 aaaggtgccc tctatggctg gtctttccac tatatcccta taggcaaaga taccaatctg
      781 gatatgatga ttaccccgga acagagggct atgcttgctt atcgtattcc tgaaatacgg
      841 gttaaaaaac ctatcttcct ggctgatttc tggaatgatg gtactttctc aggtggttgt
      901 attgcaggag gcagaagata cttccatatc aatgctaagg gagaagtgga gccgtgtgct
      961 tttgtccact ttgccataga taatattaag gggaagagtc tgaaagaagt attacgaaat
     1021 cctttattcc agtctttcca gaaacgccag cccttttctg ataatctgct ggcaccctgc
     1081 cccattatag ataaccccca acagcttaga gatattgtag cagaatcagg ggcatatcct
     1141 actcatgagg gggccgaaac cgtcttatca ggttctattg gcaaatacct ggatgaactg
     1201 tcatctaact ggcataaaaa atcaaggcca atctttgagg aaagaataaa aaaagcaata
     1261 aaagtaaaag aagaatacca gaaacatcgc atcgtagcgt agtatttaaa gataaaaatc
     1321 agtagcaaaa ctaaattgct aatttaacaa gatttaaata tttttaatta taattgtcaa
     1381 atattaacta ttacccaaaa caattggcca aaaattaaaa ctccgctctc agttttttga
     1441 ataaaatatt tcttcttttt tactttcctt tttaaatatt aatactacat aaaaatagtt
     1501 aatgtcagca gaatatagca aataagacaa aacattcagt cagctacaat aaatattgta
     1561 aatacgattt gttacagcta taattatata aaaatgtgtt ggttccttaa ttaatactct
     1621 cttctattat ctttataacc ataaaaagga gggggtgaaa tactttttgt aagcaatctt
     1681 aaaatctgaa ggcgaggagg tcttttcaga tgaaaaataa attaagatta tccggttatt
     1741 ttataagctc tctattattt tctttatatt atcctttttg ttaggctgtg gtggccccat
     1801 tccttctaaa ccaactatta attcctttac agctgctcct ccaacaatag cccccggtga
     1861 tagttctgtc ttgagctgga atgttaccgg tgccaccagt gtcaccattt ctcatgggat
     1921 tggcagtgtt tcttccacag gaactcgttc aacaagccca gccactacca ctacctatct
     1981 cctg
//
LOCUS       DAOQWT010000323         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_67081_length_1980_cov_3.563636, whole genome shotgun sequence.
ACCESSION   DAOQWT010000323 DAOQWT010000000
VERSION     DAOQWT010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67081_length_1980_cov_3.563636"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PKJ95_06395"
     CDS             <1..445
                     /locus_tag="PKJ95_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR65901.1"
                     /translation="RFLEELGIETLGTEIGIILAMLFVSMPLLVDSARDAFKWVPTRM
                     ENVARSLGATQMQTFLGVTFVLSWRGILSGMIVTWARAISEFGAVIILAYHPMIAPTM
                     IYERFTTYGMKYAVPANIILIVISIIIFVVLRLVSLGGKEKHDSR"
     gene            432..1454
                     /locus_tag="PKJ95_06400"
     CDS             432..1454
                     /locus_tag="PKJ95_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR65902.1"
                     /translation="MIHVKDLNIRLGIFRIKNVSLKVKKGEFLVLLGPTAAGKSVVLE
                     AIAGLVPVQSGKIMVNNRDITFLKPEHRNISICYQDYNLFPHMNVQDNIRYGLRFKKD
                     RNNPKYEKNFKELVKLLKLGNILERLPLNLSGGEQQRVSLSRGLIGNPDVLLLDEPLS
                     ALDPNIKTTIQEELKNIHETLHTTIIMVTHDFVEANYLAEKVAIINEGEIIEQGSSEE
                     VFQKPTSIFGAQFMGIKNIFWIEDPEELSFFGLQKPAHIGIRPENIYVSQEPLSTDYH
                     FKGTIGDIQNNQIFMEVKCCNAKREYSTYLTTNRFLELGLKKGQPIYFGFGKNYLIYF
                     REKNEV"
     gene            1727..>1980
                     /locus_tag="PKJ95_06405"
     CDS             1727..>1980
                     /locus_tag="PKJ95_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65903.1"
                     /translation="MKKTHFVSIICILILLISLVYTPALAQTDEDLPVLTVSTSYKSL
                     FSTSEQTGMLDRIVKEAFQRIGIKTEIVFNPTGRSSEDVN"
BASE COUNT          643 a          351 c          378 g          608 t
ORIGIN      
        1 acgtttttta gaagaacttg gtattgagac actgggtacc gaaattggaa ttatcctggc
       61 catgctcttt gtcagtatgc cactactggt tgattcggcg agagatgcct tcaagtgggt
      121 tcctaccagg atggaaaatg ttgctcgcag tttaggggca acccagatgc agaccttttt
      181 aggtgttacc tttgtcctct cctggagagg tattttaagt ggaatgattg tgacctgggc
      241 cagggcaatc agcgaatttg gagcagtcat tattttagca taccatccta tgatagcgcc
      301 tactatgatt tatgagagat ttaccaccta tggaatgaag tacgccgttc ctgccaatat
      361 tattctgatt gtcatcagca tcatcatctt tgtggtttta cggctggtct cactgggagg
      421 aaaagaaaaa catgattcac gttaaagacc ttaatataag attggggata ttccgaataa
      481 aaaatgttag cttgaaagta aaaaaaggtg aatttctggt attattggga cctactgccg
      541 ccggtaaaag tgtagtatta gaagccatag ccgggttagt acccgtccag agtggtaaaa
      601 taatggtcaa taaccgcgat attacttttc tcaaaccgga acatcgaaac atctctatct
      661 gctatcagga ttataatctc ttcccccata tgaacgtaca ggataacatc cgctatgggc
      721 tacgctttaa aaaagaccgt aacaatccta aatatgaaaa gaattttaaa gaattggtga
      781 aattgttgaa gctgggaaat attctggaac gtttgcccct caatttaagt ggaggagaac
      841 agcagagagt ctcgctatcc cggggtttaa taggtaatcc cgatgttttg ttattggatg
      901 agcccttatc tgccctggac cccaatatca agactaccat acaagaagag ttaaagaata
      961 ttcatgaaac cctacatacc actatcatta tggttaccca tgactttgtt gaagccaact
     1021 atctggcaga aaaggttgcc attatcaatg aaggagagat aatagaacag ggctcctcgg
     1081 aggaagtatt tcagaagccc acttccattt ttggggcaca gtttatggga ataaaaaata
     1141 ttttctggat tgaagaccct gaggaattgt ctttctttgg tttacagaaa cctgctcata
     1201 ttggcatacg gcctgagaat atatatgttt cgcaagaacc attatcaacc gactaccatt
     1261 ttaaggggac cataggggat attcagaata atcagatatt tatggaagtc aaatgttgta
     1321 atgcaaaaag agaatacagc acctatctga ccaccaaccg ttttctggaa ttagggctta
     1381 aaaagggcca acccatttat tttggttttg gtaaaaacta tttgatttat tttagagaaa
     1441 aaaacgaagt ctaatctttt ttaaaaatct actgactcta cccttccatt ataactatta
     1501 tgtatgtcta atccccaata tttataaaaa tataatatac ccttatttaa ctacgaatta
     1561 tattattatc ctcattttta ttaatattct ttaatttttg cagtaaaaca ccactttttg
     1621 ctaatataat tagaaaaatt agtaaaaggc ttgattaata aataaaagaa ggtattatta
     1681 taattgtttc agttaaaaat aaagttagag aggcataaag aaaaaaatga aaaagactca
     1741 ttttgtgtct ataatttgta ttctcatatt gctcatatct cttgtttata cccctgcttt
     1801 ggctcaaaca gatgaagact tgcccgtact cactgtcagc acctcctata aaagcctatt
     1861 ttctacatct gaacaaaccg gaatgttgga tagaattgtg aaagaagcat ttcaaagaat
     1921 tggtataaag acagagatag tatttaatcc cactggaagg tcttcagaag atgtcaatga
//
LOCUS       DAOQWT010000324         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_67646_length_1970_cov_2.805222, whole genome shotgun sequence.
ACCESSION   DAOQWT010000324 DAOQWT010000000
VERSION     DAOQWT010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67646_length_1970_cov_2.805222"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1970
                     /locus_tag="PKJ95_06410"
     CDS             <1..>1970
                     /locus_tag="PKJ95_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNR65904.1"
                     /translation="NQCGLPKKMALELFKPFVMHHLVKRKLAHNIKTAKKLIEQESPE
                     IWNILQEIVEEHPVLLNRAPTLHRLGIQVFKPVLVEGNAIQLHPLACPAFNADFDGDQ
                     MAVHVPLSTEAQTEAEILMLATNNVLSPANGIPIALPSQDIILGCYYLTMKEKVRKGE
                     GKIFANPEEAIRAQEEGHVDIHASVRVRVNDKIEDTTVGRVIFNQVLPEDMKFVDLTI
                     NKKALARIISYLYRQYGQEVTVKCLDDVKELGFKFATKSGITIGIIDLEIPSEKEKIL
                     EDAEKDVAKIKEQFNYGLIMEEERVEKIINAWTNAADKVVTAILSHLRSFNPLYMMAD
                     SGARGSRRQIGQLAGMRGLMADPSGKIIEFPIRSNFREGLSCLEYFISTHGARKGLAD
                     TALRTSKSGYLTRRLIDVAQDVIITEEDCGTNDGVFVSDIQEDGNIIEKLEERIVGRI
                     AQEDIVDSESGETIVLRGEIIDEKIAKKISDLGIKEVMIRSPLTCASKQGICVKCYGK
                     DLATGKLVTIGEAVGTIAAQSIGEPGTQLTLRTFHTGGIKITGEDITLGLPRVEQLFE
                     VRKPKKQAIISEINGFVDQIKDEPEAFRKDVIIKPETEILSGKNKEVKKKIYSIPKEL
                     RIIVEEGQKINAGQKLTTGFIDADDILRIQSI"
BASE COUNT          706 a          278 c          428 g          558 t
ORIGIN      
        1 aaatcagtgt ggattaccaa aaaaaatggc tttagagctt tttaaaccat ttgtaatgca
       61 tcacctggtt aaaagaaaat tagcacataa tattaaaaca gctaagaaat taattgaaca
      121 ggaatcacca gaaatttgga atatattaca agaaatagtc gaggaacatc ctgtattatt
      181 aaatagagcc ccaacattgc acaggctggg gattcaagtt tttaaaccgg tattggtaga
      241 aggaaatgct atacagctac atcctttggc atgtcctgcc tttaacgctg attttgatgg
      301 tgatcaaatg gctgtacatg ttcctttatc tacagaggca caaaccgaag ctgaaatact
      361 gatgctagct acaaataatg tgttatcacc agctaatggt attcctattg ctttgccatc
      421 acaggatatc attttagggt gttactactt aactatgaaa gaaaaagtga gaaaaggtga
      481 gggtaaaata tttgccaatc ctgaagaagc tataagagca caggaagaag gacatgttga
      541 tattcatgcc agtgtaagag taagggttaa tgataagatt gaagatacca ctgtaggtcg
      601 cgttattttt aaccaggttc tacctgaaga tatgaaattt gttgacttaa ccatcaataa
      661 aaaagcgctg gcacgaatta tttcttatct ttatcggcaa tatggtcagg aggtcactgt
      721 aaagtgtttg gatgatgtaa aagaattagg gttcaaattt gctactaaat caggcataac
      781 tatcggaatt attgatttag agattccatc tgaaaaagaa aaaatattgg aagatgccga
      841 aaaagatgtt gctaaaatta aggaacagtt caattacggt ttgattatgg aagaagaaag
      901 agtcgaaaaa attataaatg cctggaccaa tgctgcagat aaagttgtta ccgctatttt
      961 aagccattta aggagcttta atccacttta tatgatggct gattcgggtg cccgaggaag
     1021 cagaaggcaa attggtcagc tagcgggtat gaggggttta atggctgatc cttccggtaa
     1081 aattatagaa tttccaattc gttctaattt tagggaagga ttgtcttgtc tggaatattt
     1141 tatatctaca catggcgcaa gaaagggttt agccgataca gctcttagaa catcaaagtc
     1201 aggttactta accagaagat taatcgatgt tgcccaggat gtaattatta ctgaagagga
     1261 ttgtggtaca aatgatggtg tcttcgtttc agatattcaa gaggatggaa atatcattga
     1321 gaaattagag gaaagaatag ttggaaggat agcacaggaa gatattgtag atagtgaaag
     1381 cggtgaaact attgtactca gaggagaaat tatagatgaa aaaatagcta aaaaaatcag
     1441 tgatttaggt attaaagaag ttatgatacg ttcaccccta acctgcgctt caaaacaagg
     1501 tatctgtgta aaatgttatg gaaaagattt agcaaccggc aagctggtaa ctattggaga
     1561 agcagttggt accatagctg cccaatctat aggtgaaccg ggaacccagt tgactttgag
     1621 gaccttccat actggtggca taaagattac cggtgaagat ataactcttg gattacccag
     1681 agtagagcaa ctttttgaag taagaaaacc taaaaagcaa gcaataataa gtgaaataaa
     1741 tgggtttgtg gatcaaatta aagatgagcc agaggcattt aggaaagatg ttattattaa
     1801 acctgaaacc gaaatattat ctggtaaaaa taaggaagtt aaaaagaaga tttattctat
     1861 tcccaaggaa ttaagaatta ttgttgaaga agggcaaaaa atcaatgctg gtcaaaagtt
     1921 gaccactggt tttattgatg cagacgatat tttgagaatt cagagtatta
//
LOCUS       DAOQWT010000325         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_68084_length_1962_cov_3.602517, whole genome shotgun sequence.
ACCESSION   DAOQWT010000325 DAOQWT010000000
VERSION     DAOQWT010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68084_length_1962_cov_3.602517"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKJ95_06415"
     CDS             <1..231
                     /locus_tag="PKJ95_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65905.1"
                     /translation="KKTETEAVKKVHKSRPEKPINTTFQKNVSVHKLPSIKKPVKIVN
                     YDKTPRKRKFITEKEQNRLEHIVTRVLGPEKD"
     gene            362..1060
                     /locus_tag="PKJ95_06420"
     CDS             362..1060
                     /locus_tag="PKJ95_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNR65906.1"
                     /translation="MTISENYQKIREEIPSEVRIVVATKGRTPAEIEEVIEAGATDIG
                     QNYVQEAEAAKKAVRGDLAQRVSWHMIGPLQKNKINKALPLFDIIQTVDSLELAKEID
                     KRIIKSGKEFIPILLEINIGSEKSKYGIKPDEHERLEEYLQQLAYDIAQLAHIRLVGL
                     MTMGAFGANPEQYRQDFYRAKNIFDLLAKMRLKQVDMKYLSMGMTDSYHIAIEEGANV
                     VRIGTAIFGPRKNV"
     gene            1262..>1962
                     /gene="rlmN"
                     /locus_tag="PKJ95_06425"
     CDS             1262..>1962
                     /gene="rlmN"
                     /locus_tag="PKJ95_06425"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNR65907.1"
                     /translation="MAEKIDIKELSYQELEKIITEQGFPSYRTGQIFHWVYQKKVSSF
                     SEMSNIPHNFITRLSSLFQPNSLQCIKKMESRDGSQKFLFRLGDKHIIESVLIKDRKR
                     NTVCLSTQVGCLWHCLFCASGKEGFKRNLTAGEIISQLLAIEKISSEHISNIVFMGMG
                     EPFDNYEQLMKSIEIINEKQGINIGTRKITISTCGIIPKIKKFAQNPLQVELSLSLHA
                     AEDSIRNFLMPVNKK"
BASE COUNT          716 a          285 c          408 g          553 t
ORIGIN      
        1 aaaaaaacag aaacagaggc agttaaaaaa gtacataaat ccaggccgga aaaacccatt
       61 aatactacct tccaaaagaa tgtatctgtt cataaattac cttcaataaa aaaaccggta
      121 aagattgtta attatgacaa aacccccagg aagagaaaat tcattactga gaaagaacaa
      181 aatcgtctgg aacacattgt aaccagagtg ctggggccag aaaaagatta aaattattaa
      241 aaagtaagta attggcaact gctaatatct cttttcccct gtatattttc cttctaaatt
      301 ggtattataa ttattattaa tgattaaaaa aattgtttat ttttggaagc aggtggttat
      361 aatgacaatt agcgaaaatt accaaaaaat aagagaggaa attccatctg aggtccgaat
      421 agtagttgca actaagggta gaacacctgc cgagattgag gaagtgattg aagcaggtgc
      481 tactgatatt ggccagaact atgttcagga agccgaggct gcaaagaaag cagtaagagg
      541 tgatttagcg caaagagtaa gctggcatat gattggtccg ctgcagaaga ataagattaa
      601 taaagcgctg cccctctttg atattattca gactgttgat tctctggaac tggcaaagga
      661 aatcgataaa cggataataa aatctggcaa agagtttatt cccatattgt tagagatcaa
      721 tatcggtagt gaaaaaagca aatacggaat aaaaccggat gaacatgaaa ggctggaaga
      781 gtatctgcaa caattagcat atgacattgc tcagcttgct catatccggc tggtggggct
      841 aatgactatg ggagcttttg gggcaaatcc cgaacagtac cggcaggatt tttacagggc
      901 aaaaaatata tttgacctac tggcaaaaat gagattgaag caggtagata tgaagtatct
      961 ttccatgggg atgactgatt cttatcatat tgctattgaa gaaggtgcca atgtggtaag
     1021 aattggcact gctatatttg gtcccagaaa aaatgtttga tttgaccaaa tatttattgg
     1081 aatattttta tagcgtataa aaaagatttt ttaataaata gatgtgattt gatattgtat
     1141 gtaattaagt ggaaagtact tgattagtaa ggtgtgggtg gcagaataat gatggttagc
     1201 aataaagaat atgtgagaga actttttaga atgcattgtt gaaaagtggg agataataat
     1261 aatggctgaa aagattgata ttaaagaatt atcctatcaa gaactggaaa agataattac
     1321 cgaacaagga ttcccatctt ataggacagg gcaaatattt cactgggtat accaaaaaaa
     1381 agtgagcagt ttcagtgaaa tgagcaatat accccataat tttataaccc gattaagttc
     1441 cctctttcag ccgaatagtc ttcaatgcat aaaaaaaatg gaatcccggg acggctccca
     1501 aaaatttctt tttcggttgg gtgataagca tattattgag agtgttttaa ttaaagatag
     1561 aaaaagaaat actgtctgct tatctactca ggtgggttgt ttatggcatt gtcttttttg
     1621 cgccagtggg aaagaagggt ttaaaagaaa tctgacagcc ggagaaatta taagccagct
     1681 gttagctatt gaaaaaatta gtagcgaaca tatcagcaat attgttttta tgggtatggg
     1741 agaacctttt gataattatg aacagctaat gaaatccatt gagataatca atgagaaaca
     1801 gggtataaac attggtaccc ggaaaataac catttctacc tgcggaatca ttcctaaaat
     1861 taaaaaattt gctcaaaacc cccttcaggt agaactctcg ttatccctgc atgctgccga
     1921 agatagtata aggaattttt tgatgccagt taataagaaa ta
//
LOCUS       DAOQWT010000326         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_68113_length_1962_cov_2.618773, whole genome shotgun sequence.
ACCESSION   DAOQWT010000326 DAOQWT010000000
VERSION     DAOQWT010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68113_length_1962_cov_2.618773"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /gene="ltrA"
                     /locus_tag="PKJ95_06430"
     CDS             complement(<1..1718)
                     /gene="ltrA"
                     /locus_tag="PKJ95_06430"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HNR65908.1"
                     /translation="MCKKQQLRVPDNEEELRQLQDDFYKTAREGFMKNELVGFKNLKE
                     IAFCKANIITAIHKLKANKGSRTPGVDDEVMRENILEQDYEEVITRVMKTVDNYISKP
                     IRRAYIPKTGSNDMRPLGIQCLIDKIVQECIRNVIEPILEAQFFDHSYGFRPLRDAHQ
                     AYERVKKILHGTGYHWVLEGDITQFFDNVNHNVLTGALYHMGIKDRRILQMIKAMLRA
                     GIMNEIYYNDIGTPQGGILSPLLANVYLNKFDNFIVREWEEKKLKNVSRYNKSGKRKG
                     QKSGVIQQTLKRAAPNMKPAYLIRYADDWVIVTNTLNNAKKLKSRIHNFLKTHLKLEL
                     SDKKTRITNVKKKAINFLGFLIRARAGKSKSGLIIHGKPDKEKLVRKVLKLKKNIHDF
                     KKSANIENLIHKINLYNCRVRGILNYYKVASHIYLGVRKIAYELSGLVFKVLRSCKAP
                     VKWIPADETRNLTEIHKIRKQLIPAIKYKDFWIGVTNLNFVKWELVSLKNPKETIYTA
                     EGRELYRRRTGKQRRLNRKDLTLSIKYSEIISHSKKNSKSIYNFEFFMNRGYVYNVDK
                     GRCRVCG"
BASE COUNT          536 a          389 c          262 g          775 t
ORIGIN      
        1 ccacatactc tacatctacc tttgtcaaca ttatagacat aacctctatt cataaagaat
       61 tcaaaattat aaatagattt actgtttttc ttagaatggg aaattatttc cgagtattta
      121 atagaaagtg ttaaatcctt cctgttcaat cttcgctgtt tacctgttct acgtctgtat
      181 aattccctac cttctgcagt atatatagtt tccttcggat ttttaagact tactaattcc
      241 cactttacaa aattcagatt ggttacacct atccagaaat ccttatactt tatagctggt
      301 atcaactgtt ttcgtatttt atgaatttca gttagatttc ttgtttcatc tgctggtatc
      361 catttaactg gtgctttaca acttcttaga actttgaaca ctaaaccact taattcataa
      421 gctatttttc taactcctaa ataaatatgg cttgctactt tgtaataatt taatattcct
      481 cgaactctgc aattataaag gtttatttta tgaattaaat tttctatatt agctgacttt
      541 ttaaaatcgt gaatattctt tttaagtttt aaaacttttc tgacaagttt ttctttatcg
      601 ggttttccat gtattattaa gccactttta cttttacctg ctcttgctct tattaagaat
      661 ccaagaaaat ttatggcttt tttctttaca ttggttatcc tggttttctt atcagataat
      721 tccagtttaa gatgggtttt caaaaaatta tgaattctac ttttgagctt ctttgcgttg
      781 ttcaaagtat tagtaacaat aacccaatca tctgcataac gtattagata ggcaggtttc
      841 atattaggag ctgctctttt aagagtctgc tgaataacac cgcttttctg tcctttacgt
      901 ttgccacttt tgttatatct ggaaacgttc tttaactttt tttcttccca ttcacgaact
      961 ataaaattgt caaatttatt gagataaaca tttgcaagca gaggtgaaag aattcctccc
     1021 tgtggtgtgc caatatcatt gtaatagatt tcattcatta tacctgctcg taacattgct
     1081 tttatcatct gaagaattct tctgtctttt atacccatat gatatagtgc tcctgttaga
     1141 acattatgat taacattatc aaagaattga gtaatatcac cttctaatac ccagtggtat
     1201 cctgtgccgt gaagtatctt tttaaccctt tcataggctt gatgggcatc ccgtaatggt
     1261 ctaaaaccat aactgtgatc aaaaaactga gcttccaaaa ttggttctat tacattacga
     1321 atgcattcct gaactatttt gtcaattaga cattgaattc ctaagggtct catatcatta
     1381 cttccggtct ttggtatata agctcttctt ataggtttag aaatataatt atcaactgtt
     1441 ttcataactc ttgtaattac ttcttcataa tcctgttcaa gtatattttc tctcattact
     1501 tcatcatcta ctcctggggt tctactgcct ttattggctt ttagcttatg tatggctgtg
     1561 atgatattgg ctttacaaaa ggctatctct tttaaatttt taaatcccac taattcattt
     1621 ttcataaatc cttctcttgc agtcttgtaa aaatcatcct gtagttgcct gagttcttct
     1681 tcattgtctg gaactcgcaa ttgttgtttc ttacacataa agaatcatct cctctctgtg
     1741 caagattgta atcaggtttg cacatcggct tcctgcgaac agttaattaa acctaaaatc
     1801 ataacccgac tatgcccctt cgctccatta tctttcgata acttcatcac tactatgggc
     1861 tgctgactca ggaattaaac ggtcatttcc taccgttgag gtctcattgc ctgctttacc
     1921 aggctacacc tttcctgtct caatcgttcc ttacacttca gc
//
LOCUS       DAOQWT010000327         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_68646_length_1953_cov_2.477871, whole genome shotgun sequence.
ACCESSION   DAOQWT010000327 DAOQWT010000000
VERSION     DAOQWT010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68646_length_1953_cov_2.477871"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..625)
                     /locus_tag="PKJ95_06435"
     CDS             complement(221..625)
                     /locus_tag="PKJ95_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNR65909.1"
                     /translation="MHQKEKELFSELISIVTRLRGPQGCLWDRKQTIDSMAINIAEEA
                     QEVTEAVANKNYTNLCEELGDLLMDILIEIQIAQEAGLFDYEQVLTGAREKFIRRHPH
                     VFGDVKVNNAEEALSVWKKMKEMEKENKNSRS"
     gene            complement(1094..>1953)
                     /locus_tag="PKJ95_06440"
     CDS             complement(1094..>1953)
                     /locus_tag="PKJ95_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65910.1"
                     /translation="IEIGLDIPNVNTIIIDDAHQFGLSQLYQLRGRVGRSSRRAYAYL
                     LYPSQKGLSENARRRLDAIREFSDLGSGFRLAMRDLEIRGAGNLLGKEQHGFVSDVGF
                     NYYCQLLEESINQILKVDTDTDKGQDEEPEIEVKLESYIPEYYISNPELRISYYQRLS
                     QIKAEAELNDIKRELRDIYGSYPKEVENLLLVIQVKLKLKNQGVTNIRVTSHHISLKY
                     RVGSLLENKIKKALKNEPKKNISYSQKGSHYELKFVLEKTGLPACSSNIFNQILPFLE
                     KAIPNNGDY"
BASE COUNT          610 a          402 c          277 g          664 t
ORIGIN      
        1 aaccaagatt gttttctaaa gcaatttcga tacattcttc taaatccaga ttcaggattt
       61 ctgaagaaca aacatttttc caggaaaata aaaaaagaca gaaaatcaag gtaataaaaa
      121 aagtaaccca aaccaacttt tcttttttaa ataatgtgag cagatacata gataaacctc
      181 ctttcaaatt taaagataag gtagtaataa aatggcatta ctaagaccga ctgttcttat
      241 tttctttttc catctctttc atctttttcc agactgagag tgcctcttca gcattattaa
      301 cctttacatc cccaaaaaca tggggatgac ggcggataaa cttttctctg gcaccggtca
      361 agacctgttc ataatcaaat aaacctgctt cttgagcaat ctgtatctca atcaggatat
      421 ccatcaataa gtctcccagt tcttcacata agttggtata attcttgttc gctactgctt
      481 cggttacttc ctgtgcttcc tcagcaatgt taattgccat tgagtcgatg gtttgtttcc
      541 tgtcccagag acagccctga ggacctctga gtcgggtaac gatggaaatt aattctgaaa
      601 acaattcttt ctctttttga tgcattataa ttttgctcct tctgatattg agttaaaatc
      661 agtttttgtt cattagtttt taaagttaat cgttcatggt ttttagtttt tagtaagata
      721 cgataaaaca ccacccttaa attcacttgc ttaagtctaa tggagacatg ttggaaatat
      781 gtcgtgacaa tcacattcaa ttatgcccac actcttttct ttatctttgt attttaccaa
      841 aaactaaaaa ccataaacca aaaactttat aaccaacgac taacgactat ttaatgcttt
      901 cacatatgcc cacactcttt gcctatgcat tttgactaaa aactataaac tataaacaat
      961 aagctgaatt attaactgaa ttataataac attataatag taaattttta ataacatttt
     1021 aattaaatat aaaatattta taaaaatcgc aactatttct cccagaagca agaggattat
     1081 accatctgta attctaataa tctccgttgt tgggaatagc tttttctaaa aagggcaaaa
     1141 tctgattgaa aatattactg gaacaagccg gtaggccagt tttttctaaa acaaatttta
     1201 gttcataatg agagcccttc tgagaatagc ttatattctt tttaggttca tttttcaagg
     1261 cttttttaat cttattctcc aagagagaac caacccgata ttttagagaa atatggtgag
     1321 aggtaaccct gatattggtt acaccctggt ttttgagctt taactttacc tgtattacca
     1381 gaagcaaatt ttctacctct ttcgggtatg aaccgtaaat atcccgtagt tctcttttga
     1441 tatcattcag ttccgcctct gcctttatct gacttaatct ctggtaataa cttattctca
     1501 attcagggtt tgaaatgtag tattccggaa tatagctctc cagtttaact tctatttcag
     1561 gttcctcatc ctgcccttta tccgtatcag tatccacctt tagaatctga ttgatactct
     1621 cttctaataa ctggcagtaa taattaaaac caacatcact tacaaaacca tgctgttctt
     1681 tccccagcag attaccagct cctcttattt ctaaatcacg catagccaat cgaaacccag
     1741 aacccaaatc gctgaattct ctaattgcat ctaatcttcg cctggcattt tcactcaagc
     1801 ctttttgaga agggtaaagc agataggcat aggctcttcg gctactgcgt ccaacccttc
     1861 ccctgagctg atataactgc gacaacccaa actgatgggc atcatcaata attatggtat
     1921 tcacattggg tatgtccaga cctatttcga taa
//
LOCUS       DAOQWT010000328         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_69036_length_1946_cov_2.813855, whole genome shotgun sequence.
ACCESSION   DAOQWT010000328 DAOQWT010000000
VERSION     DAOQWT010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69036_length_1946_cov_2.813855"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PKJ95_06445"
     CDS             complement(<1..534)
                     /locus_tag="PKJ95_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNR65911.1"
                     /translation="MKKYRIIFIVTILFSIFFLLYNTSICSPASEESVYKERREKMVA
                     QDLAARDITDPLVLEAMGKVPRHEFVDARVKDEAYGDFPLGIGEGQTISQPYIVALKT
                     QLLELTGAEKVLEIGTGSGYQAAILAEIVKEVYTIEIYESLSLKAQSLLNKLGYDNIK
                     FLIGDGYYGWQDEAPFDA"
     gene            875..1165
                     /locus_tag="PKJ95_06450"
     CDS             875..1165
                     /locus_tag="PKJ95_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNR65912.1"
                     /translation="MDKEEMDLLIQKAKDRDKEAMMQLIEQFNPLIKKQAAFFKGMGL
                     EYEDAYQQGILYFITGVYEYRPMPPVTFAGYMKKRIKWGLWMYWRKLFKTGE"
     gene            1425..1700
                     /locus_tag="PKJ95_06455"
     CDS             1425..1700
                     /locus_tag="PKJ95_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR65913.1"
                     /translation="MDGYVENYVENMILEECLDELDKQEHQIVVLYYWWGYRDAEIGK
                     ILGESQQMINYRRNRALKKIKSLLEVRTLSRFGGRRGLSGQRWWHYV"
     gene            complement(1633..>1946)
                     /locus_tag="PKJ95_06460"
     CDS             complement(1633..>1946)
                     /locus_tag="PKJ95_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNR65914.1"
                     /translation="PQEAAQVVWDHLRLKLGTGLIIANPVPTEFALSKKKVDHWIEQA
                     RKLAHKENIKGKQLTPYLLNKVKELSEGRSLKANIELLKHNATIAARIALSYHQIGIK
                     S"
BASE COUNT          575 a          317 c          447 g          607 t
ORIGIN      
        1 agcatcaaag ggtgcttcat cctgccagcc atagtacccg tcacctatta ggaattttat
       61 attgtcataa cccagcttgt ttagcaggct ttgagcctta agagataagg attcataaat
      121 ttcaatggta taaacctctt tcactatttc tgctaagatg gcagcctggt atccggaacc
      181 ggttcctatt tccaggactt tttcagctcc ggttaactcc aaaagctggg tctttagagc
      241 aactatatag ggttgagaga tggtctgtcc ttcgcctatt cccaggggga aatcgccata
      301 tgcctcatcc ttgacccgag catctacaaa ttcatggcga ggcactttac ccatggcttc
      361 cagtaccaaa gggtctgtaa tatcccgtgc agctaaatcc tgggcaacca tcttctcacg
      421 acgttcttta tagacagatt cctctgaggc tggtgagcag attgaggtat tatatagcaa
      481 gaaaaaaatg gaaaatagta ttgttactat aaatattatt ctatattttt tcattttacc
      541 aaccaacttt tattaaaaag taaaaatatt gaatttttac cagtcaggca atttttaaag
      601 aataatcttc ttgtagtaat taatattacc cttttctctg ttctttttca aggttatccc
      661 caatatgttt acactaaatc aaaagattat acagttttta ctaacattag agtaaatttc
      721 agcaataagc ccttttccga ttcttattat actgtttttt tcgcaaaact ttactaaaat
      781 tatcctctat ttttacccat tatagactac aagagagcat ctttttagct attctaaatc
      841 agaaacagag aaaatagtag aagggaagga aattatggat aaggaagaga tggacctatt
      901 gattcagaaa gccaaagaca gagataagga agcgatgatg caattaatcg agcagttcaa
      961 tcctctcata aaaaaacagg cagcattttt taaagggatg gggctggaat atgaggatgc
     1021 ctatcagcag ggaatattat attttattac cggtgtttat gagtatcgac ctatgccccc
     1081 ggttaccttt gccggatata tgaagaaaag gattaaatgg gggttgtgga tgtattggag
     1141 aaaattgttt aagacggggg aataattggg agaagtcgtt agtcgatggt cgttggtatt
     1201 tagttaaaag atgtgggaat aaatgaaggt ggtcgttggt cgatggtcga tggtagttag
     1261 ttataaagat gtgggtgtag atggaagggg tttatagttt tagataaaga gtgtgggctg
     1321 taggggtgag attgccacgg tttgctttca gcaaacctcg caatgactaa gataaagatg
     1381 ttgttggaaa tgaaagaata aaaggaggag ggggggaagc taagatggat ggttatgtag
     1441 aaaattatgt agagaatatg attctggaag aatgcctgga tgagctggat aaacaggaac
     1501 atcagatagt agtgctatat tactggtggg ggtatcggga tgccgaaatt ggtaaaattt
     1561 tgggtgaatc gcagcagatg attaattata gacgcaacag agctttaaaa aagataaaaa
     1621 gtctcctgga ggtcaggact ttatcccgat ttggtggtag gagagggcta tccgggcagc
     1681 gatggtggca ttatgtttga gaagttctat attggctttt agacttcttc cctcactgag
     1741 ttcttttact ttattcaaga gatagggggt aagctgtttg cccttgatat tctccttatg
     1801 agctaatttg cgggcctgtt ctatccaatg gtctactttc tttttactta atgcaaattc
     1861 ggtgggtacc ggattggcta ttatcagacc ggtgcccagt tttaatcgta gatggtccca
     1921 gaccacctgt gctgcttcct gtgggt
//
LOCUS       DAOQWT010000329         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_69241_length_1943_cov_2.226695, whole genome shotgun sequence.
ACCESSION   DAOQWT010000329 DAOQWT010000000
VERSION     DAOQWT010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69241_length_1943_cov_2.226695"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKJ95_06465"
     CDS             complement(<1..623)
                     /locus_tag="PKJ95_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNR65915.1"
                     /translation="MKKSNRVLVALSGGIDSSVAAFLLKEKGYQVAGLYLHLIEDPKN
                     STLSDSRVRAQEVASKLALPLYQIDYREDFCRIIIKDFMEQYRRGLTPNPCIFCNEKV
                     KFRLLYDFAFKEGFDYIATGHYVRVKKEGQEDRYLLKRGIDRKKDQSYFLYRIKESIL
                     SKCIFPLGDLKKEDTRKIAGNIELPDYIKGESQEICFIPGNDYRKLL"
     gene            complement(991..>1943)
                     /locus_tag="PKJ95_06470"
     CDS             complement(991..>1943)
                     /locus_tag="PKJ95_06470"
                     /inference="COORDINATES: protein motif:HMM:NF037782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HNR65916.1"
                     /translation="KKILEQGIEVIGVYFTIPFLKNEPDYQGEKHLNIPLLYVDLGRD
                     FQEILLNPKFGFGKNINPCIDCRILMFKKAGALMIQEGASFIISGEVLGQRPMSQSKK
                     NLFLISEESGFGKYILRPLSALLLPETEPERCHIVKRGELLDIQGRSRKRQIDLARQW
                     GIENYPSPAGGCKLTEPGYAHRIKDLLIKGKYDFNDLELLKVGRHFNIYKFVKLVVGR
                     NEKENKIILSLASPEDYLLNSPTHKGPVALVRCYNNLAEKNEEIDNILDLASKITTRY
                     CDKEESADLIDINISKNNKIIKKLKQVPSLSEQSIKNYLI"
BASE COUNT          568 a          388 c          297 g          690 t
ORIGIN      
        1 atcaacagct tacgataatc attaccgggt ataaaacata tttcctggct ctccccttta
       61 atatagtctg gtaattcaat attaccggca atttttctgg tatcctcttt tttcagatca
      121 cccagtggga aaatgcattt ggatagaata gattctttta tcctatacag aaaataggac
      181 tggtcttttt ttcggtctat tccccttttt aaaagatatc tgtcctcttg cccttctttt
      241 tttacccgga cataatgacc ggtagcaata tagtcgaatc cttctttaaa ggcaaaatca
      301 taaagtaatc taaacttcac cttttcgtta cagaaaatgc aggggttggg ggtcaaccct
      361 cttctatatt gttccataaa gtcctttatt atgattctac aaaaatcttc tctataatca
      421 atttggtata aggggagagc taattttgag gctacctctt gtgctctcac tctactatca
      481 gatagggtgc tgtttttagg gtcttcaatc agatgtagat agagacctgc aacctgatat
      541 cctttttctt ttagcaggaa tgcagcaaca ctgctatcta ttcctccgct gagtgccacc
      601 agaacacgat tagatttttt cataaatttt cctcattaat acaaatagaa taatcagtcg
      661 ttggtcattg gtatttagtt ttaaaatgca aagataaata atatggagta catttgatga
      721 gattgctacg tcacttcgtt cctatccttg acaaattcat tctgtatatt acttttatat
      781 ctgtataccc taaaaacggt aacctataaa tatctttttc tcattgtgga gtagccgatg
      841 aaccttagtt taagattaaa aagcatttta gtaaacttgc ccatacgcgg ataatatatt
      901 tattttgtgt ttaatcagcg aatagcaatc gcatacccta tcactcacat tattgaacta
      961 taaacttaaa actataaact attagctttt ttatataagg tagtttttta tagattgttc
     1021 ggaaagagat ggtacctgct tcagcttttt tatgatctta ttgtttttac tgatattaat
     1081 atcaattaaa tctgcgcttt cttctttatc gcaatacctg gttgtaatct tgctggctaa
     1141 atccaaaata ttatctatct cttcattttt ctcagccagg ttattataac aacgtactaa
     1201 ggcaacaggc cctttatggg taggggaatt aagcaaataa tcctctggac tggctaaaga
     1261 aagaattatc ttattctcct tttcatttct acctaccact aatttaacaa atttataaat
     1321 attaaaatga cgtcccacct ttaacaattc caggtcattg aagtcatatt tgcctttaat
     1381 cagcaaatct tttattctgt gggcatatcc cggttcggtt aatttacatc ctcctgccgg
     1441 ggaagggtag ttctcaattc cccattgccg ggctaaatct atctgccttt ttctagaacg
     1501 gccttgaata tcgagaagtt ctccacgttt tactatatga catctctccg gttctgtctc
     1561 tggtaataat agagcagaga gaggcctcaa aatatatttt ccgaatcctg actcttcact
     1621 gattagaaag aggttttttt tactctggga catcggcctc tgtcccaata cctctccgct
     1681 aatgataaaa gatgctcctt cctgaatcat caacgcacca gcctttttaa acatcagaat
     1741 acgacagtca atacagggat ttatattttt tccaaaaccg aacttgggat taagcaatat
     1801 ttcctgaaaa tctctaccta aatccacata cagcaaagga atattaaggt gtttttctcc
     1861 ctggtaatct ggttcgtttt ttaaaaaagg gatagtaaaa tatacaccta ttacctcaat
     1921 tccctgttct aagatttttt tga
//
LOCUS       DAOQWT010000330         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_69459_length_1939_cov_3.671444, whole genome shotgun sequence.
ACCESSION   DAOQWT010000330 DAOQWT010000000
VERSION     DAOQWT010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69459_length_1939_cov_3.671444"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PKJ95_06475"
     CDS             <1..798
                     /locus_tag="PKJ95_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65917.1"
                     /translation="TAKLVKLLEVPVVTVSIKGGYLAYPRWAKRSRKGKVDLDYNLCL
                     SKNECGELSLENIRKRIRDYLSYDEVDYQKKECNKYTGRKLAENLEYLLFACPNCHSF
                     NTLISHGDKLICQQCQYGVIYNQYGFLEGIQSKLYFDNLRDWNYWQKDFLREYINERY
                     ISAQNLEYILKDKEVSIFVGNLNHPFVYEGKGNLSLLKRELYFEDIEGGKHSFELREI
                     IGLSVQFKAVLEFNYRDNMYRFEFHNPHLSAYKWVLAINFTREKIEG"
     gene            complement(834..>1939)
                     /locus_tag="PKJ95_06480"
     CDS             complement(834..>1939)
                     /locus_tag="PKJ95_06480"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HNR65918.1"
                     /translation="DVRGALGMWIADMDFLCPSEVIAAVKKRAEHGIYGYISPDAVEA
                     FKEAAAGWFERRYNYRVPTDWMLFISGVVPSINAAILEFTEPTDGVIIQNPVYYPFSD
                     SIINCGRTIINNQLIENNGFYTMDLNGLEELAKDPKTKLIILCNPHNPVGRVWTEDEL
                     YQAGKICSDNGVLIFSDEIHADFIMKGHKFVTAGKLSSEISENLIISFAPSKTFNIAG
                     LAASLIVIPDKAIREKLEKRMNINNYPAPNVFAPIAGEAAYLYGDNYVDELIDYIEKN
                     FDYVIEYLKENLPAIEMKKPEGTYLGWVDFRKTGINHKEIYSIVLEKAKLAVDLGEWF
                     GTGGEDFLRFNFACPRTTVETAMERLKVAFQNL"
BASE COUNT          607 a          355 c          361 g          616 t
ORIGIN      
        1 accgcaaaat tagtaaaatt actggaagtg cccgtagtga ctgtgagcat aaagggtgga
       61 tacctggctt atccacgctg ggcaaaaagg tcaagaaagg gcaaggttga tttagattat
      121 aacctgtgtc ttagtaaaaa tgaatgtggt gaactttcat tggaaaatat taggaagagg
      181 ataagagatt acctttctta tgatgaggtg gattatcaga agaaggaatg caataagtat
      241 actggcagaa aattggcgga aaatctggaa tatcttttat ttgcctgccc gaattgccac
      301 tcttttaata ctcttatttc tcatggcgat aaattgattt gccagcaatg tcaatatggt
      361 gttatctaca atcaatatgg ttttttggag ggcatacaaa gcaaactgta ttttgataat
      421 ctcagggatt ggaactactg gcagaaagat tttttgagag aatatattaa tgagagatat
      481 atttcagctc aaaatcttga atatatttta aaagataaag aagtctcaat atttgtgggc
      541 aatcttaatc atccttttgt ttatgaaggt aaaggaaacc tatctctact aaaaagagaa
      601 ttgtattttg aagatataga agggggaaag cattcatttg aattaagaga gattataggc
      661 ttaagtgttc aatttaaagc agttctggaa tttaattata gagataatat gtatcgcttt
      721 gagtttcaca atccccattt atctgcttac aaatgggtgc ttgccatcaa ttttacccgg
      781 gaaaaaatcg aagggtagaa acagaaaacc cgtcattaat taaaatcaag aaattaaaga
      841 ttttggaagg caactttcaa tcgttccatg gctgtctcta cggttgtcct ggggcaggca
      901 aaattgaatc ttaaaaagtc ttctccccct gttccaaacc attctcccag atctaccgct
      961 aattttgctt tttctaagac tatggaatat atttctttat gattaattcc ggtttttcga
     1021 aaatcaaccc atcctaaata tgtgccttcc ggttttttca tctctatagc cggtaaattt
     1081 tccttgagat attcaatcac atagtcaaag ttcttctcta tataatctat taattcatcc
     1141 acataattat ctccataaag ataagcagcc tcacctgcaa taggtgcaaa tacattggga
     1201 gcaggataat tattaatatt cattctcttt tccaatttct ctcttattgc tttatctggt
     1261 attacaatta atgaggccgc cagcccggct atattaaatg ttttgctggg cgcaaaggag
     1321 atgattagat tttcactaat ttccgatgac aattttcctg ctgtaacaaa tttatgtcct
     1381 ttcattataa agtcggcatg aatctcatca gagaaaatca ataccccatt atctgaacaa
     1441 attttaccag cctggtaaag ctcatcttca gtccagaccc tgcccaccgg gttgtgtggg
     1501 ttgcagagaa taatgagttt cgttttaggg tccttagcca gctcttctaa tccattgagg
     1561 tccatagtat aaaagccatt gttttcaatt aattggttat ttataatggt tcttccacaa
     1621 tttatgatac tatcactgaa tggatagtaa acaggatttt gaataatgac cccatcagtg
     1681 ggttcggtga actccaggat agcagcattg atggagggca ctacaccact gatgaagagc
     1741 atccaatcgg taggtactcg atagttataa cgcctctcaa accatccagc agcagcttct
     1801 ttaaaagcct ctactgcatc gggagagata tagccataga taccatgttc tgctcttttt
     1861 ttgactgctg cgataacctc agaggggcat aaaaaatcca tatcagctat ccacataccc
     1921 aaggctcccc ggacatcat
//
LOCUS       DAOQWT010000331         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_69669_length_1935_cov_4.233511, whole genome shotgun sequence.
ACCESSION   DAOQWT010000331 DAOQWT010000000
VERSION     DAOQWT010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69669_length_1935_cov_4.233511"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="PKJ95_06485"
     CDS             <1..1138
                     /locus_tag="PKJ95_06485"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNR65919.1"
                     /translation="IRKAARLNPRATIVVTGCAVQSNLLEIKEYKNISLIVGNRDKEK
                     IFDIINQIPLSEEEPHIFSSSPEKINGYIESKCISSSSRIRKMVKIQDGCDQFCSYCI
                     IPYLRGRARSRPIEQILSEIDYLANRNCKEVILLGINLGSYGSDLKGNRVNLADIIRR
                     IDSISNIKRIRLSSIELPYIDESLIKAFRCYPKLCHHLHIPLQSGDNRILHLMNRKYT
                     SEQYSETINLLKRDIPDIAITTDIIAGFPGEDEAAFQNTLNLAKKMGISKMHIFPYSK
                     RRFSLASFLPDTIDEKVIKNRSEQLINLSNELRRDYIFQNLGKEKHILIEFLGKEKNG
                     SFAYGLTDNYIKVCVNDVNYEKGQMVKVRIIKVNDSYAVGEAIN"
     gene            1472..>1935
                     /locus_tag="PKJ95_06490"
     CDS             1472..>1935
                     /locus_tag="PKJ95_06490"
                     /inference="COORDINATES: protein motif:HMM:NF020118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HNR65920.1"
                     /translation="MMNIDLVKILDEIEKEKGIPKEAILEAIEVSLVSAYKKNFTQGK
                     DNVEINIDKESGRVRVLSKKIVVETISKPSSEIDLEQAQQYKKGVVKLGDEIEVEITP
                     EEFGRIATQTAKQVVVQKIREAERKAIYERYIDKVRDIVTGVVQRQEHFNYI"
BASE COUNT          718 a          257 c          388 g          572 t
ORIGIN      
        1 gattagaaag gctgcccgtt taaatccacg ggcaaccatt gtagtaaccg gttgcgcagt
       61 gcaatctaat ttgcttgaaa taaaagaata taagaatata tctctaattg tagggaacag
      121 agataaagaa aaaatttttg acataatcaa tcaaatacca ttatcagaag aagaaccaca
      181 tatttttagc agctcacctg agaagattaa tggctatatt gaaagcaaat gtattagcag
      241 ttcaagtcgt attcgtaaaa tggttaaaat ccaggatggt tgtgaccagt tttgcagcta
      301 ctgtattatc ccctacctgc gtggcagagc cagaagcaga cctattgagc agatattgtc
      361 tgaaattgat tatttagcta atagaaattg caaagaggta attttgctgg gcattaatct
      421 gggcagttat ggctctgatt tgaaaggcaa cagagtaaac ttagcagata taatcagaag
      481 aatagacagt atttccaata tcaaaagaat cagattgagt tcaattgaat taccttatat
      541 tgatgaaagc ctcataaagg cctttcgctg ttatcccaag ctttgtcatc atcttcacat
      601 tcccttacaa agtggcgata accgcatact tcatcttatg aatagaaaat atacttctga
      661 acaatatagt gagactatta atcttcttaa aagagatatt cctgacattg ctattactac
      721 agatataata gcggggtttc ccggtgagga tgaagcagca tttcagaata ctctaaattt
      781 ggcaaaaaag atgggcatca gtaagatgca tattttccct tattccaaaa gaaggtttag
      841 cctggcttct tttttacctg atactattga tgaaaaagta ataaaaaata ggagtgaaca
      901 gctgattaac ctttccaatg aacttcgcag ggactatatt tttcagaatt taggcaaaga
      961 aaagcatatt ttgattgaat ttctgggaaa ggaaaagaat ggtagttttg cctatggctt
     1021 aactgataat tatataaagg tctgtgttaa tgatgttaat tacgaaaagg gccagatggt
     1081 taaggtgaga ataattaaag tcaatgacag ctatgcagta ggtgaagcaa taaattagct
     1141 ttattttgaa gcaagaggat aaaaataatt tgagtcaatg gtttgtttgg tataaaatgt
     1201 ttaggtaaaa taatagtgta aaagatagtg taaaatgtta gtgtaaaatt taggatttga
     1261 tattttttat ttctgtgtta taataatata aattttaaaa acatcctgtt ttagaaaaga
     1321 gtggtttgtg aaccactctt ttgttttgta attttatttt tcaattaaaa tcggggacat
     1381 ttttaaaaaa tttagtaaca gaaaagaaga ttagaaaagc ggttaagaga ttctgctctc
     1441 ccaccctaaa gaggagagga ggttaacaaa tatgatgaac atcgatttag ttaaaatact
     1501 tgatgaaata gaaaaagaaa aaggaatacc caaagaagct atcctggaag ccatagaagt
     1561 atctctcgtt tcagcttata aaaagaattt tactcagggt aaggataatg tagagataaa
     1621 tattgataaa gagagtggaa gagtacgggt cttatcaaaa aaaatagtag ttgaaacaat
     1681 aagtaagcct tcttcagaaa ttgatttaga acaagctcag caatataaaa aaggggtagt
     1741 taaactggga gatgaaattg aggtggaaat aacacctgag gaattcggca gaattgccac
     1801 ccaaactgcc aagcaggttg ttgttcagaa gataagagaa gcagagagga aggcgatata
     1861 tgaaagatat attgataaag tgagagatat cgtaacagga gttgttcaac gccaggaaca
     1921 tttcaactat ataat
//
LOCUS       DAOQWT010000332         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_69670_length_1935_cov_4.043617, whole genome shotgun sequence.
ACCESSION   DAOQWT010000332 DAOQWT010000000
VERSION     DAOQWT010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69670_length_1935_cov_4.043617"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKJ95_06495"
     CDS             complement(<1..324)
                     /locus_tag="PKJ95_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012168296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65921.1"
                     /translation="MIRKNILTIVNKKRLFFQMLIIVFLMLPSSNYFSLAQVDVREAI
                     VKVYTVSNYPDYYNPWSMEGPRASSGSGCIIEKNLILTNAHVVSNQTFLQVRKYGDTQ
                     RYRAKV"
     gene            complement(366..1262)
                     /locus_tag="PKJ95_06500"
     CDS             complement(366..1262)
                     /locus_tag="PKJ95_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR65922.1"
                     /translation="MELKKITENTYYIPGPTNLGLFVEDKKVILIDSGNDEWVGRKIF
                     RLLEEREWELSKIINTHSNADHIGGNAYLQKKTNCDILATALESVFIDEPSLEPFLLW
                     GASPFKALRNKFLQAQPSRVTHIIGEEGIISGTPLQAFPLEGHFIQMIGVKTPDEVLF
                     LADSLFSTETISKYGLTVKSDIEKSLNTYSLLEKLSARLFIPSHTAPTKDISDLIRVN
                     RENVEQINRKILYYCSEPVSRERLIQLLINDYQITLSSVQFVLTHITVSAYLSYLYDK
                     QLIKPIFEEGKMLWTSNDKIDL"
     gene            complement(1289..1732)
                     /locus_tag="PKJ95_06505"
     CDS             complement(1289..1732)
                     /locus_tag="PKJ95_06505"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HNR65923.1"
                     /translation="MPDSDDLRKAKEILKKGNYTFVLTKKGQLLKTSYERGILPMLRI
                     IKEDEQALCNAIVADKVIGRAAAFLLIKYRVKAVYADKISEKAREVLDNNSIFYQFGE
                     SVPFIKNRDQTGQCPLEKLTSHTDNPDYACEQISLFYKQALGIEL"
BASE COUNT          579 a          374 c          315 g          667 t
ORIGIN      
        1 tacttttgcc cgatatcttt gtgtatctcc atatttcctt acctgtaaaa aagtttgatt
       61 gcttaccaca tgagcattgg tcagaatcag atttttttca attatgcatc ctgaaccgga
      121 gctggcacgt ggcccttcca tactccaggg attgtaataa tcggggtaat tacttaccgt
      181 gtaaacctta acaattgctt cacgaacatc gacttgtgcc agagaaaaat aatttgacga
      241 agggagcatc aaaaagacaa ttatcagcat ctgaaaaaac aatctttttt tattaacaat
      301 tgttagaata ttttttctta tcataagttt tccacctcat ttctgataca aaaattcatt
      361 ttttgttata aatcaatttt atcattactt gtccatagca ttttgccctc ttcaaatatg
      421 ggctttatca actgtttatc gtacaaatag ctgagatacg cagaaacagt aatatgagtc
      481 agtacaaatt gaacactgga aagtgttatc tgataatcat tgatcaacag ttgtattaac
      541 ctctctctgg agacaggctc gctgcaataa tacaaaattt ttctgttaat ctgttccaca
      601 ttttccctgt ttacccgaat taaatcactg atatccttgg taggtgcggt atgggatggg
      661 ataaatagtc tggcagaaag tttttctaaa agagaatagg tgttaagtga tttttcaata
      721 tcgcttttca ctgttaagcc gtatttacta atggtctccg ttgaaaagag gctgtcagca
      781 aggaacagta cttcatcagg ggttttcact ccaatcatct gaataaaatg accttccagg
      841 ggaaaagcct gcaaaggtgt tccactgata attccttcct ctccaataat atgggtgacc
      901 cgggaaggct gtgcttgcag aaatttgttt ctgagagctt tgaaaggaga tgctccccac
      961 agtaaaaaag gttctaaaga tggttcatca ataaagaccg attccagagc agtagccaga
     1021 atgtcacaat tagtcttttt ctgtaaataa gcattgccgc caatgtggtc agcattggaa
     1081 tgagtattaa taattttact cagttcccac tctctctctt ccagtagtcg gaatattttt
     1141 ctgccaaccc attcgtcatt tccactatca atcagaataa cttttttatc ttccacgaaa
     1201 aggcccagat tagttggtcc ggggatataa taggtgtttt cagtaatttt ttttaattcc
     1261 ataatcttct ccaacaattt tgttactttc agagctcaat ccccaatgcc tgcttataaa
     1321 aaaggctaat ctgttcacag gcataatcag gattatcagt atgagaagta agcttttcca
     1381 gaggacattg gccggtctgg tcacgatttt taataaaggg aacactttct ccaaactgat
     1441 aaaagataga attattatcc aatacttccc tggctttctc actgatttta tcggcataaa
     1501 ctgctttcac tctatatttt atcagcaaga aggctgcagc acgaccaatt accttatctg
     1561 ccacaatcgc attacaaagg gcttgttcat cctctttaat aattcgtaac ataggaagaa
     1621 tgcctctttc ataagacgtt tttaataact ggcctttttt ggttaacaca aaggtatagt
     1681 tgcccttttt tagtatttcc ttcgctttac gcaaatcatc tgaatccggc atgacttttt
     1741 actcttaata catattttta tatttatttg tattatgttc tatgattaca ttatatacat
     1801 ctttttatgt acatatcaag aggttgtttc tctccaatac tttttcaata tagcaggatc
     1861 tttattattt attaacctgc tatagtattc tagctagcaa ggttaaaata ttttagaata
     1921 aattaaaagc atcac
//
LOCUS       DAOQWT010000333         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_70576_length_1920_cov_3.416622, whole genome shotgun sequence.
ACCESSION   DAOQWT010000333 DAOQWT010000000
VERSION     DAOQWT010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70576_length_1920_cov_3.416622"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKJ95_06510"
     CDS             <1..339
                     /locus_tag="PKJ95_06510"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNR65924.1"
                     /translation="ISEMKNLVEQIKKEKNRKKIIQLDAEFHNLFNKSTGNEVLAENL
                     EKLRNRLGRLWYLAEKSENYSLKIPEELEGIIKALEERDEKRCKQLLQEHAIHFIETI
                     KVNLYNNNLV"
     gene            395..1348
                     /locus_tag="PKJ95_06515"
     CDS             395..1348
                     /locus_tag="PKJ95_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNR65925.1"
                     /translation="MKNILVIGATGQIGSELTLALRDAYGSANVVAGGHKRKPSSEKL
                     QDSGPYEIADCLAPKVLAALIEKYKIDTIYHLAALLSATSEKNPKLAWEVNIQGLINV
                     LDLAKSYQCAVFFPSSIGVFGDSTPKDNTPQDTIQRPSTIYGITKLTGELLCDYYYHK
                     YNLDVRGVRYPGLISYETLPGGGTTDYAVEIFYEALAHKEYTCFLKEDTYLDMMYMPD
                     ALKAAMQLMETESNRLIHRNGYNVSAMSFSPREIFGEIRKWIPEFKISYEIDPIRQSI
                     ADSWPNSMDDNVARQEWGWQPEYDLPKMTKDMIEKLKLKLK"
     gene            1648..1764
                     /locus_tag="PKJ95_06520"
                     /pseudo
     CDS             1648..1764
                     /locus_tag="PKJ95_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1812..1920
                     /locus_tag="PKJ95_06525"
                     /pseudo
     CDS             1812..1920
                     /locus_tag="PKJ95_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449135.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
BASE COUNT          641 a          338 c          426 g          515 t
ORIGIN      
        1 ataagcgaga tgaaaaacct ggtggagcag ataaagaagg aaaagaacag gaagaagata
       61 attcaactgg atgcagagtt tcataatttg tttaataagt ctactggtaa tgaggtgctg
      121 gcggagaatt tagagaaact gagaaacaga ttgggacgcc tctggtatct ggcagaaaaa
      181 tcagaaaatt actctctaaa aattcctgaa gagctggagg gtataataaa ggcgttagaa
      241 gagcgggatg aaaaaagatg taaacaatta ttacaggaac atgccattca ttttatagaa
      301 acgattaagg tcaatcttta taataataac ctggtataag cacaagacag ttataaaaat
      361 ttttcttctc aaaaaaatca tagggaggaa ttttatgaaa aatatactgg taattggtgc
      421 tacgggacag attggttctg aattaacttt agccttacgg gatgcctacg gttcagccaa
      481 tgtagtggca ggaggtcaca aaaggaaacc atcttctgaa aaattacagg attcgggacc
      541 atatgagata gctgattgtc ttgctcccaa agtgcttgcg gcgttaatag aaaaatacaa
      601 aatagatacc atctaccatc tggcagcctt gctttcagcc acttccgaaa agaatcccaa
      661 attggcctgg gaagttaata ttcagggttt aatcaatgta ctggatttag caaaatctta
      721 tcaatgtgct gtcttcttcc ccagttccat cggggttttc ggtgacagta ctcccaaaga
      781 taacacccct caggatacca ttcaacgacc ctctaccatc tatggtatta caaaattaac
      841 cggtgagcta ctctgtgatt attattacca taagtataac cttgacgtgc gcggtgtacg
      901 ttatcccggt ttaatctctt atgaaacact acctggtgga ggtactactg attatgcagt
      961 ggagatattc tatgaagccc tagcccataa agaatacacc tgctttttaa aagaagatac
     1021 ctatctggat atgatgtata tgcctgatgc cctgaaggct gctatgcagc ttatggagac
     1081 agaaagtaat cgtcttatac accgtaatgg ctataatgtc agtgctatga gcttttctcc
     1141 ccgagaaatt tttggggaga tccggaaatg gattcctgaa tttaaaatta gttatgagat
     1201 agaccccata aggcagtcta tagcggattc ctggcccaac agtatggatg ataatgtagc
     1261 gcgccaggag tgggggtggc agccagaata cgatttgccc aagatgacta aagatatgat
     1321 agagaagtta aagttaaaat taaaataaaa aaaattaaca taatccttac tatcattgat
     1381 tcttaaacca ttttgtattt tttttcttaa atttagaaat aggaggatat gccgataatg
     1441 agtataaaga aattagaaaa accattacag gaatctctgc aaaatttgaa agaagagggg
     1501 cgcttaaaag gcaaagagtt cattattatc gatatcagaa aagcggaagg tgaaagtgga
     1561 ccgcgttact ttttaaaggg acagggaaaa aaaccattta tcaggatgaa ttccaattct
     1621 tatctgggtt tatccttgcg agaagatatg gttttagcag gagaagaggc agcccatcaa
     1681 tttggcaccg gaccgggggc agttcgtttt ataagcggta cctttcaagt ccatagagaa
     1741 ttggagcaaa gattggcaaa attccataag cgggatgatg ccataatttt taactctgcc
     1801 tacacaaccg tgctgggggt gctatcccct aaatcgagaa tctcaaggat gacgcaatca
     1861 agcgcttcca ggagcaggtt gaactggtca atatcatgga ggccgaggac ctcggcgcca
//
LOCUS       DAOQWT010000334         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_70884_length_1915_cov_4.012903, whole genome shotgun sequence.
ACCESSION   DAOQWT010000334 DAOQWT010000000
VERSION     DAOQWT010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70884_length_1915_cov_4.012903"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKJ95_06530"
     CDS             complement(<1..206)
                     /locus_tag="PKJ95_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR65926.1"
                     /translation="MLTMRQKMAVTGELRDRYQKSKKREKITILNEFTKLTGYNRSYA
                     SQILNQSKLLGYVHVAGQTIKYIR"
     gene            447..1058
                     /locus_tag="PKJ95_06535"
     CDS             447..1058
                     /locus_tag="PKJ95_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNR65927.1"
                     /translation="MSTHQKEICTHSIEELPNNISLAKIFWTFFRIGLFTFGGGLAMG
                     TVIRYELASKNKWVTDDEFLSEFATATVIPGSIAVNIAYLQGYRFRGKIGAATAVMGT
                     ILPSFITIFAIAYFGLPYFNHPKVVAFFRGSALAVAGQLLFTGFIFGKKLLVKWKNIV
                     ICLISLLMATVLGLHPIWSLITAAILDYFLFTKNNHTSVDPDC"
     gene            1083..1625
                     /locus_tag="PKJ95_06540"
     CDS             1083..1625
                     /locus_tag="PKJ95_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNR65928.1"
                     /translation="MMIVSMKILIDLIESFMVIGLSACGGGLVTIPLIQHEMVNVRHW
                     LVSNDIAKILSIAQMTPGPIAINAATFVGFKIAGIIGSVMATLAVIFPSLIILITISS
                     FLKNIKQNEAVIKINQGIQAGVLSLILFAIWSFGSTVISSTLDLFVTIAAFLMLLFLK
                     NKIHPVLVIVLCGIVGLIIY"
     gene            complement(1662..>1915)
                     /locus_tag="PKJ95_06545"
     CDS             complement(1662..>1915)
                     /locus_tag="PKJ95_06545"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNR65929.1"
                     /translation="IDKLLDVCSNAREIVLVGASTPMYPKAFLGTNVSILAGSWWNNR
                     LKADLFKKISLSGGIHHIQNYMIKKAVMVKRREANTKPV"
BASE COUNT          548 a          347 c          332 g          688 t
ORIGIN      
        1 tctcgtatat acttgatggt ctgtccggct acatggacat aaccgagtaa tttactctga
       61 ttcagtatct gactggcata ggaacggttg tagccggtaa gttttgtaaa ttcattcaat
      121 atggttattt tctccctctt cttggacttc tggtatctat cccgtaattc tcctgttact
      181 gccatcttct gcctcatggt taacatctcc cttgcctcct cactatttgg aaagatttgt
      241 tcttccaata tagaggagat tgtcctataa taaaaattat ttcatttaga tttttatatg
      301 aggcatcgat actatttcat atatattttt gatgaggcaa ttcggatatt tgacatcacc
      361 cctatcttca tttactatta atctattaaa aatatgagga aataaaataa ctctaataaa
      421 taattgaaga agaggttaac acacatatgt ctacccatca aaaagaaatt tgcacccata
      481 gtatagagga attacctaat aatatttctc ttgctaaaat attttggacc ttctttcgga
      541 ttgggttatt cacttttggt ggaggcctgg ctatgggtac agtaataaga tacgaactgg
      601 cttcaaaaaa taaatgggtt actgatgatg aattcttatc tgaatttgcc actgctactg
      661 ttattcctgg ttctattgcg gttaatattg cctatctgca gggctatcgt tttcgaggaa
      721 agattggagc agccactgcg gttatgggca caatcctgcc ttcctttata actatcttcg
      781 ctattgccta tttcggattg ccctatttta atcaccccaa agttgtggcc ttcttcagag
      841 gaagcgcact tgctgttgct ggtcagcttc tctttactgg attcattttc ggtaaaaaac
      901 tactggtgaa atggaaaaat attgtaatat gtttaataag tcttttaatg gcaactgtac
      961 taggcttaca tcctatctgg tccttaataa cggctgctat acttgattat ttccttttca
     1021 ctaaaaataa tcatacctct gttgacccag attgttgatt aaaactttaa gaaactggag
     1081 atatgatgat tgtttcaatg aaaattttaa ttgatcttat agagagtttt atggtcattg
     1141 gcttaagtgc atgtggggga ggattggtta ctattcctct tattcaacac gaaatggtta
     1201 atgtcagaca ttggctggtt tctaatgata ttgcaaagat tctatcaatt gcccagatga
     1261 ccccaggacc catagcaatc aatgcagcaa ctttcgtggg cttcaaaatt gctggtataa
     1321 taggttcggt tatggcaact ttggcagtta tcttcccatc tcttatcata cttataacta
     1381 tttcatcttt tttaaaaaat attaaacaaa atgaagcagt tattaagatc aaccaaggaa
     1441 tacaggcggg tgttttaagt ttgattctgt tcgcgatctg gtccttcggt tctacagtta
     1501 tttctagtac tctggattta tttgttacta ttgcagcttt tttgatgttg ctttttctaa
     1561 agaataaaat acatccagta cttgttatcg tactttgtgg tattgtgggc ttaataattt
     1621 attaaattgg aagatagata aatattcccc ccccttcatt atcagacagg ttttgtattt
     1681 gcttcccttc ttttaaccat tacagctttt ttaatcatat aattctggat atgatgtatt
     1741 ccaccagaaa gagagatttt tttgaacaaa tctgccttta aacgattatt ccaccaggag
     1801 ccagccaaaa ttgaaacatt tgtacccaga aatgctttag gatacatagg agttgatgcg
     1861 ccaaccagaa caatctctct agcgttagag caaacatcta ataatttatc aatag
//
LOCUS       DAOQWT010000335         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_71410_length_1907_cov_2.649028, whole genome shotgun sequence.
ACCESSION   DAOQWT010000335 DAOQWT010000000
VERSION     DAOQWT010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71410_length_1907_cov_2.649028"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKJ95_06550"
     CDS             <1..205
                     /locus_tag="PKJ95_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNR65930.1"
                     /translation="SNIRHRELLLKVKECLGNLINSLEEGIQADFVAMDLKHITNQLA
                     NITGESYDQEMLNRIFCQFCIGK"
     gene            507..671
                     /locus_tag="PKJ95_06555"
     CDS             507..671
                     /locus_tag="PKJ95_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65931.1"
                     /translation="MLISDSKSSKDYQFSTMPNYSKKKTFLPDVLKRNIYQSRNHIDE
                     IAEIGEQLLI"
     gene            958..1737
                     /gene="rsmG"
                     /locus_tag="PKJ95_06560"
     CDS             958..1737
                     /gene="rsmG"
                     /locus_tag="PKJ95_06560"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNR65932.1"
                     /translation="MEKEKIYAYSEEDLRRYIDLGALSLGLNLTEDEINKFIVYLKMI
                     NLYQKKVNITSLKNPLDIINKHFLDSLSAVNIVSNLFKNYSKGKRMKIIDVGSGAGFP
                     GIPIKIVLSDVRMILLEARENKTLFLNEVIDQLRLTDIRVEQDRAENIGKVVEFREEY
                     HIVLSRAVATLEVLSEYCLPLCPVNGVMLAFKGSSYPEELSASTKVIERLGGILESIN
                     TVRIPYSNHFRYLLVIRKRKSTPEDYPRKNGVPLKRPLYFE"
BASE COUNT          705 a          261 c          375 g          566 t
ORIGIN      
        1 tagcaatata aggcacagag aactactatt gaaggtaaaa gaatgtttag gtaacctgat
       61 aaatagcctg gaggaaggga ttcaggctga ttttgtggca atggatttaa agcatattac
      121 caaccagcta gctaatatta ccggcgaatc atatgaccag gagatgctaa accgtatatt
      181 ttgtcagttt tgcattggta aataatagta ataaatcaaa aaaatgcttt tttaatatat
      241 aaattgtctg gacaagtgtg gtatgatgca cccaaacaat aaaaagtaaa atacaaatcc
      301 aagaaagttt aaatcgcaaa atattctgag attaaaatgt tccacgtgga acataaaggc
      361 caattatgtt tgaaaaattc ttccaaaaaa aagtatatgg ttaacttatt ttctaaaata
      421 aaaggtatta aagtaaaaaa tatatttttt tttaaaaaga ttttttaaaa tagcaggtta
      481 aaaataacaa tttgttactt tccccgatgc tgatatcaga tagtaaatca agcaaggatt
      541 atcaattctc tacaatgcct aactatagta aaaagaaaac atttctgcca gatgtattga
      601 aaagaaacat atatcagagc agaaatcata tcgatgaaat cgctgaaatt ggcgagcaat
      661 tattaatcta aataaactta tagagatatg gatataatgg tgtggggaga acggtgacgc
      721 acattgaaaa ttaatctgtg taaaagctgt ggaaaagtaa caaaagccac catcaggttt
      781 gttgaggaat aggagagagc gggaaaataa gaatatttcc agcagggagt atagtacaat
      841 ataggctata aaaagcttgt aattccttat ctttatagtg aagtgtatca aatatcgtgg
      901 tcttcacact cgtcatccca tagtagaaag acacttaaaa ggagctgtgg aaaaactgtg
      961 gaaaaagaaa aaatatatgc ctatagtgag gaagatttaa ggcggtatat tgatttaggt
     1021 gctctgtctt tagggttgaa cctgacagaa gatgaaatta ataagtttat cgtttattta
     1081 aaaatgataa acctttacca gaaaaaagtg aatataacct cacttaaaaa tcctctggac
     1141 attattaata aacatttcct ggactcgttg tctgcggtta atattgtgag taatctattt
     1201 aaaaattatt ctaaggggaa gaggatgaaa atcattgatg tcggtagcgg agcagggttt
     1261 cccggaatcc ctatcaagat tgtattatct gatgtaagaa tgattctatt ggaggcaaga
     1321 gaaaataaaa cattattctt aaatgaggtt atagaccagc taaggctaac agacatcaga
     1381 gtggaacagg acagagctga aaatattgga aaggtagtag aatttagaga ggaatatcat
     1441 attgttttat ctagagccgt tgctactctg gaggtcttat ctgaatactg ccttccctta
     1501 tgtccggtaa atggtgttat gctcgccttt aaaggtagtt cctatcctga ggagctgtcc
     1561 gccagcacta aagttattga aagattagga ggaattctgg agagtatcaa taccgttaga
     1621 attccttatt ctaaccattt tcggtatctt ttagtcatac gtaaaagaaa atcaactcca
     1681 gaagattatc cccgaaaaaa tggtgtaccc ctgaaaagac ctttgtattt tgaatgattt
     1741 tttggtaata ttataataaa tcataaaata ttgttttttt ggagaatggt atcgagcggt
     1801 atgttccacg tggaacatat attttaatat aaggggattt agccataatg agtaaaatta
     1861 tagctatcag taatcagaaa ggtggggtag gaaagacaac caccgcc
//
LOCUS       DAOQWT010000336         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_71809_length_1900_cov_3.386450, whole genome shotgun sequence.
ACCESSION   DAOQWT010000336 DAOQWT010000000
VERSION     DAOQWT010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71809_length_1900_cov_3.386450"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKJ95_06565"
     CDS             complement(<1..425)
                     /locus_tag="PKJ95_06565"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF014351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNR65933.1"
                     /translation="MEKQKPKLILITGGARSGKSSFAESLAQKMGENIIYIATAQALD
                     KEMRERIAEHRKRRPKNWQTVEETVQVDKVIKKVGTQTEVIIIDCMALLISNLISDFN
                     EKSSKDIFASKVQEKIEHIMEASLKCQATVIIVTNEVGSG"
     gene            complement(517..1572)
                     /gene="cobT"
                     /locus_tag="PKJ95_06570"
     CDS             complement(517..1572)
                     /gene="cobT"
                     /locus_tag="PKJ95_06570"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNR65934.1"
                     /translation="MNNRFEEIIGEIPSIDNTILKDTLNHFDKLAKPRKSLGKLEEIV
                     ARAAAIYGTANPAIDRKVIFLMAADHGVTNEQVSAYSSEITYQMVLNFLKGGAAINVL
                     ARHFGVDIVVTDIGVNGDLSDKNGIIHKKIAFGTNNMALEPAMSREMAERSIEIGFEV
                     FEESFARRPIDLIGLGEMGIGNTTASSAIISLLTGAKVEDVVDCGAGLPQDTVQRKIE
                     VIKKAISLNQPDPYDAISILAKVGGFEIGGLVGCIFAAAKRRVPIVMDGLISGACALL
                     ALRLNPVIEDYLFTSHCSREKGHKISLDHLGMFPIFDLKMFLGEGTGAIFGINFIEAG
                     FKLLNEMTAFDNLRNNK"
     gene            complement(1576..>1900)
                     /locus_tag="PKJ95_06575"
     CDS             complement(1576..>1900)
                     /locus_tag="PKJ95_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNR65935.1"
                     /translation="PHLPLPGGESMSDFEKRVLSGLEKVISEYDNNNECETIAIVCHG
                     GVTRIIIGKALNIPLDKIWFIKQDSTNLNILHYYKEDIYFVETINDINHLNANPISIE
                     KGCID"
BASE COUNT          560 a          407 c          285 g          648 t
ORIGIN      
        1 cctgagccca cctcattagt tactatgata actgttgcct ggcattttaa agaagcctcc
       61 ataatatgtt caattttttc ctggacctta gaggcaaaaa tatctttaga agatttctca
      121 ttaaagtctg agatgagatt agagattagc agagccatgc aatcaataat gattacctct
      181 gtttgagtac caactttttt aataacttta tctacttgca cagtctcctc aactgtctgc
      241 cagtttttag gtcttctctt acgatgctca gcaatccttt ccctcatttc cttatcgagg
      301 gcctgtgctg ttgcaatata gataatattc tcacccatct tttgagcaag tgattcagca
      361 aatgagctct taccacttct tgctccaccg gtaattagaa ttagttttgg tttctgtttt
      421 tccattctct aaaaactcga aagatgaata ttaattttta actttttaat ataaaaggaa
      481 ttaatttaca ctacaggtag aattattttt catctatcat ttgttatttc ttaaattatc
      541 aaaggcagtc atttcattga gcagcttaaa ccctgcttcg atgaaattga tgccaaatat
      601 agcacctgtt ccctcaccca gaaacatttt caggtcaaaa ataggaaaca ttcccagatg
      661 gtcaagtgaa atcttatgtc ctttttcccg ggaacaatgg ctggtaaaaa ggtaatcctc
      721 tatcaccggg tttaatctta atgccagtaa agcacaggca ccggaaatta atccatccat
      781 tactattggt acccttcttt ttgcagcagc aaaaatacat cctactaacc cacctatttc
      841 aaacccacct acttttgcta aaatgcttat tgcatcataa ggatcaggtt gatttaaaga
      901 aatagctttt ttaataactt ctattttacg ctggactgta tcttgtggaa gccctgctcc
      961 acaatctact acatcctcaa ccttggctcc agttaacagg ctgataatgg cgctactagc
     1021 agttgtgtta ccaatgccca tttcccctaa tcctattaga tctataggcc tcctggcaaa
     1081 ggattcctca aatacttcaa aaccaatttc tatcgacctt tcagccattt ctctgctcat
     1141 tgctggttct aaagccatgt tattggttcc aaaggcaatt tttttatgaa tgataccatt
     1201 tttgtcagaa agatctccat tcactccaat atctgttacg actatatcta cgccaaaatg
     1261 gcgcgccagc acatttatag cagcaccacc ctttaaaaaa tttaatacca tctgataagt
     1321 aatttctgaa gaataggcgc ttacctgttc attggtaact ccatggtcag cagccattag
     1381 aaatattact tttctgtcta tagcgggatt tgcagtacca taaatggccg ccgcacgggc
     1441 tactatctct tccagtttcc ctaaactttt tcgaggttta gccagtttgt cgaaatgatt
     1501 tagggtgtct ttcaggatag tgttatcaat agagggaatt tctccaataa tttcttcaaa
     1561 cctattgttc atcagttaat caatacatcc cttttctatt gatattggat tagcattcaa
     1621 atgattaata tcattaatag tttccacaaa atagatatct tctttatagt aatgaaggat
     1681 atttaaatta gtagaatctt gttttataaa ccagatttta tccagaggaa tatttaaagc
     1741 cttaccgata ataatcctgg taaccccacc gtggcagaca atagctatag tttcacactc
     1801 attattatta tcatattcgg aaattacttt ttctaaacca cttaatactc ttttttcgaa
     1861 atcagacata ctttctccac cgggaagagg cagatgtggt
//
LOCUS       DAOQWT010000337         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_71885_length_1899_cov_2.700108, whole genome shotgun sequence.
ACCESSION   DAOQWT010000337 DAOQWT010000000
VERSION     DAOQWT010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71885_length_1899_cov_2.700108"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PKJ95_06580"
     CDS             <1..503
                     /locus_tag="PKJ95_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNR65936.1"
                     /translation="SLNMRDRVLILTGLTFGTRISESLSLTFGNVEGSYLHINSKKGS
                     ENISFPVPSSYKSAIEDLKTYYKSQGIEIKPDTYLFLSRKGDNMPITRIQASRIVKET
                     CISLGIDGKVNTHSFRKTFVTKIYNLTGHDIAQTKVYSRHKSLSNLDYYINTTQETNL
                     INELAW"
     gene            516..638
                     /locus_tag="PKJ95_06585"
     CDS             516..638
                     /locus_tag="PKJ95_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65937.1"
                     /translation="MGNEKRLTLMYLDACYEGAENPFIRFYYWLKLKLFEFLYR"
     gene            649..993
                     /locus_tag="PKJ95_06590"
     CDS             649..993
                     /locus_tag="PKJ95_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65938.1"
                     /translation="MNIKDKIYTEQFLMKLSKEQMDYLVYFFGKKNLSRKVREFLIKA
                     VREEHKNKRLPVIETLSGEIDPVMEYSLNQKGDKVEVTIYENGMDRDPLLYENIDEAM
                     KYVRSIDAKKRK"
     gene            999..1445
                     /locus_tag="PKJ95_06595"
     CDS             999..1445
                     /locus_tag="PKJ95_06595"
                     /inference="COORDINATES: protein motif:HMM:NF039645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6075 family protein"
                     /protein_id="HNR65939.1"
                     /translation="MDICFLNSDHERRYKDYCNRAHIYPDDRERKAMFYIMAGSNDLI
                     SKGINNFYNFKENIVKFSPGPEEMEQDFEKFSFCTSSRALTMLALNLYNSGYSSLSVS
                     DTFSYLDGDNMRLAMEAIKLRFNISLTAIEADPQEAIEQKIKNCLN"
BASE COUNT          677 a          277 c          378 g          567 t
ORIGIN      
        1 atagtcttaa tatgagggat agagttctta tattaacagg attgacattt ggtaccagaa
       61 tatcagagag tttatcctta actttcggta atgtggaggg aagctatctt catataaata
      121 gcaagaaggg atctgaaaat atatcatttc ctgtaccatc cagttataaa tctgcaatag
      181 aagatcttaa gacctattac aagagtcagg gaatagaaat aaaacctgac acttatttat
      241 tcctttcccg gaaaggtgac aatatgccca tcacaaggat acaagccagc cgaattgtaa
      301 aagaaacctg tatttcactt ggtatagatg gcaaggtaaa cactcattct ttcagaaaaa
      361 catttgtaac gaagatatat aatctcactg gtcacgatat agcacagact aaagtctata
      421 gcagacataa atctttatca aatctggact attatattaa caccactcag gaaaccaatt
      481 tgattaatga attggcatgg taggaggaat aacctatggg taatgaaaag aggctgacat
      541 taatgtacct ggatgcctgt tatgaagggg ctgaaaatcc cttcatcagg ttttactatt
      601 ggttgaaact gaagttattt gaatttttat atagatagga gtagaagagt gaatataaaa
      661 gataagattt acacagaaca gtttttaatg aagctgtcta aggaacagat ggactatctt
      721 gtttattttt ttggtaagaa gaatttatca aggaaggtcc gagaatttct tataaaagct
      781 gtcagagaag agcataaaaa taaaagatta cctgtaattg aaaccttgtc aggtgaaata
      841 gatcctgtaa tggaatattc ccttaatcag aaaggggata aagtagaggt cactatatat
      901 gagaatggaa tggatagaga cccattatta tatgagaata ttgatgaagc tatgaagtat
      961 gttagatcca tagatgccaa aaaaaggaag tgatattatt ggacatctgt tttttaaact
     1021 cagaccatga acgaaggtat aaagattact gcaatagggc ccatatttac ccagatgaca
     1081 gggaaaggaa agctatgttt tatatcatgg ccgggagtaa cgacctgatt tcaaaaggga
     1141 taaataactt ttataatttc aaagagaaca tagttaagtt tagtccaggc ccggaggaga
     1201 tggaacagga ttttgaaaag ttctctttct gtacttcatc cagggcactc accatgctgg
     1261 cgttaaactt gtataacagt ggatattctt cgctgtctgt gagtgatacc ttcagctatc
     1321 tggatgggga taacatgagg ctggctatgg aagcaataaa gctgagattt aatatttccc
     1381 tgactgccat tgaagcagat ccacaagagg caatagaaca gaagataaaa aactgtctga
     1441 attgattacc ttttaccctg aagagagaag ggaggagatt aaaattcctt gctgttttat
     1501 aattttatta ttttacgtac tcatattaat aatatatagc tgtcgtttta tcatgcacat
     1561 tacaagacaa tcttcattcg aagagtggga aggggtattt cccacatata gcaggaggga
     1621 aattgaaaaa aaagaaaatg aacagggaaa gtatgactga ctttttgata tccactttat
     1681 tatttgtctt acatttaata catattaaaa tatatttgta aattctattg aaaaaataat
     1741 acctttatac tataatagac aatgtcagat gataatacac agttaatgcc ttcctcttta
     1801 attacagagg gaagcaggtg acaagggctg tggtctactg accgggcttt aactttaata
     1861 atccgttgaa agcgaatatg gagttttaaa actctgtat
//
LOCUS       DAOQWT010000338         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_71928_length_1898_cov_2.927835, whole genome shotgun sequence.
ACCESSION   DAOQWT010000338 DAOQWT010000000
VERSION     DAOQWT010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71928_length_1898_cov_2.927835"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /gene="glnQ"
                     /locus_tag="PKJ95_06600"
     CDS             <1..183
                     /gene="glnQ"
                     /locus_tag="PKJ95_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205351.1"
                     /note="similar to ATP-binding component of ABC
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HNR65940.1"
                     /translation="MVVVSHEMGFARAVANDIIFMENGIIVEEGDPETMFHHSRNPRT
                     QEFLHKITELYGGRPK"
     gene            180..836
                     /locus_tag="PKJ95_06605"
     CDS             180..836
                     /locus_tag="PKJ95_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNR65941.1"
                     /translation="MSFLELTWNILPKLLEGTAITIELTLIAITIGILMGIPVALGRV
                     YGNKSIYTICTFFVEIIRGTPLLTQLFILYFGLPSIGIMLSPFKAALIGMGINSAAYQ
                     AEYFRGAIQSVKEEQLIAAYSLGMRQGQAIRYIVLPQMFRLVIPSWSNELIYLLKYSS
                     MAYMIQAPEIMSQGRLIASRNFRTFEVFIVVALIYLVLVLVLSKLLDLMERRFSIPGL
                     "
     gene            934..1806
                     /locus_tag="PKJ95_06610"
     CDS             934..1806
                     /locus_tag="PKJ95_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNR65942.1"
                     /translation="MILKAIKKIPGVLLVLTFLLTIIILPSWAAPEKIIVGTSADWPP
                     FEWVDSNNNFVGFDMDLLKVIAKSNGYDVEILDIGFDSLIPALQSGKVDLLAAGMTLT
                     EERAAVADASNTYWSGNQGVLVREDSELNIATALSGGNSIGAQRGTTQADWLEDNLVQ
                     KGVNVKSLELYETNDLGIMDLVNKRIEVFVADTPAAEAFEKTNPIKIVGTINTEEQYV
                     FYVQKGDPKGILPMVNEGLTRIQMSSIWNNLVNAYFIGDLTKISDCYAKFGSLLDEDV
                     EAFAKNLAQCMMSQ"
BASE COUNT          571 a          343 c          391 g          593 t
ORIGIN      
        1 atggtggttg tttcccatga aatgggattt gcccgggcag tggctaatga cattattttt
       61 atggaaaatg gtatcattgt agaagagggt gaccccgaaa cgatgtttca tcattcccgg
      121 aatcccagga ctcaggaatt tttgcacaag attactgaac tttatggagg gagacccaaa
      181 tgagttttct ggaattaacc tggaatatct taccaaaatt attagaaggc actgccataa
      241 ccatagagtt gaccttaatc gctattacca taggtatcct gatgggaatc ccggttgccc
      301 tgggtcgggt ttacggcaat aaatcaattt atactatctg cacttttttt gtggaaatta
      361 ttcgcggtac ccctttgctt actcagcttt ttatcctcta tttcggactc ccctcaatag
      421 gtatcatgct ctctcctttt aaggcagctt tgattggaat gggaataaat agcgctgctt
      481 atcaggcaga atacttcaga ggggcaatcc agtcggtaaa agaagaacag ttaatcgctg
      541 cctattcttt gggaatgaga caaggtcagg ctattcgcta cattgtttta ccacagatgt
      601 ttcgcctggt aatcccttcc tggtcaaatg aactcatcta tttattaaaa tattcttcta
      661 tggcttatat gattcaggca ccagaaataa tgtctcaggg aaggttaatt gcctcacgca
      721 atttcagaac ttttgaagtt tttatcgtgg ttgccttaat ctatcttgtt ctggttttgg
      781 tgttatccaa actgttagac ctgatggaaa gaagatttag tattccaggc ttataggcaa
      841 attggtaatg ggggtgaaaa aagaagatta aagatacaga atgttgattt atggtattaa
      901 ataaaaatta tttcttatgg aggagataaa aaaatgattt taaaagcaat taaaaaaata
      961 cccggtgtac tgttagtttt gacttttctc ttaactatca ttatcttacc ttcctgggct
     1021 gcccctgaaa agatcatagt aggcacctct gctgactggc caccctttga atgggtcgac
     1081 tctaacaaca actttgtagg atttgatatg gacctcttaa aagtaatcgc aaaaagcaat
     1141 ggttatgatg tggaaatact ggatattggt tttgattctc ttatacctgc ccttcaatcc
     1201 ggtaaagttg atttactggc agccggtatg actttaacag aggaaagagc tgcagtagcc
     1261 gacgcttcta atacttactg gtccggaaat cagggagttt tggttcgaga agattcagaa
     1321 ctgaatattg caactgccct gtccggaggg aattctattg gtgcccagag aggaacaacc
     1381 caggccgatt ggttggaaga caacctggtc caaaaagggg taaatgtaaa atctttagaa
     1441 ttatacgaaa caaatgatct gggaataatg gatctggtga ataagcgaat cgaggtattc
     1501 gttgcagaca ctcctgctgc agaagctttt gagaaaacta atcccattaa aatcgtgggt
     1561 accatcaata ccgaagaaca gtatgttttc tatgttcaaa aaggtgaccc caaaggaata
     1621 ttgcctatgg ttaatgaagg gttgaccagg atccagatgt cctccatctg gaacaacctg
     1681 gtaaatgcct atttcatcgg agatttaacc aaaatttctg actgctatgc caaattcggt
     1741 tcacttttag atgaagatgt agaggctttt gccaaaaacc tggctcagtg catgatgtct
     1801 cagtaaaaaa ttttatattt ctataaaatt ttaaaagcac acttatttta atcataattt
     1861 caaatatgtt ttatttaaac tattaaattt attcatca
//
LOCUS       DAOQWT010000339         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_71999_length_1897_cov_2.733442, whole genome shotgun sequence.
ACCESSION   DAOQWT010000339 DAOQWT010000000
VERSION     DAOQWT010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71999_length_1897_cov_2.733442"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKJ95_06615"
     CDS             complement(<1..346)
                     /locus_tag="PKJ95_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR65943.1"
                     /translation="MRKSKDERKMRKEIHLEHSSLKYPLPEQFVQELSEELAFMNEYP
                     SGGSYQTLSAKLAEYTGIAPDCILPANGSDEVIESITRAFGKKLILIPIPTFSQYEVS
                     AERNGMNKKFISC"
     gene            complement(377..1441)
                     /gene="msrA"
                     /locus_tag="PKJ95_06620"
     CDS             complement(377..1441)
                     /gene="msrA"
                     /locus_tag="PKJ95_06620"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNR65944.1"
                     /translation="MKNILIVILTIFLIIFFTTGSVFGQTITEKATFAGGCFWCIEAA
                     FEKVEGVLKAISGYTGGKTDNPTYEEVSTGKTGHYEAVEVVFDPAMVSFEELLAVFWK
                     NIDPTDVGGQFADRGSQYQTAIFYHNDEQKLAAELSKQELQNSGRFDSEIVTKILPAA
                     EFYPAEEYHQDYFQKNPIGYQMYHIGSGRADYIKSKWSGDAMENKWDEHYQKPSKEEL
                     ERILTPLQYEVTQENGTEKPFQNEYWNDKREGIYVDIVSGEPLFSSTDKFDSGTGWPS
                     FFKPIDEHFIIKREDKSYMMVRTEVRSRYADSHLGHLFNDGPAPTGLRYCINSAALCF
                     IPKESLEKEGYGQYHFLFDR"
BASE COUNT          579 a          378 c          303 g          637 t
ORIGIN      
        1 aacaagagat aaatttttta ttcattccat tccgctctgc actaacctca tactgggaaa
       61 aagtaggaat gggaatgagg attaatttct ttccaaaagc tctggtgata gattcaatta
      121 cctcatcaga accattggcc ggcagaatac aatccggtgc aatacccgta tattcagcta
      181 atttagcaga cagagtttga taagaacccc cactggggta ttcattcata aatgccagct
      241 cttcagacaa ttcctgcaca aattgctcag gcagggggta tttcaggctg gaatgctcta
      301 aatgtatttc ctttctcatc tttctctcat ctttactttt tctcaactat tcttttactt
      361 tattaagaat taatgtttac ctgtcgaaca gaaagtggta ttgcccatat ccttcttttt
      421 ctaaactttc ttttggtata aagcacaagg cggcagaatt gatacagtag cgcaatccgg
      481 ttggagcagg gccatcgttg aagaggtgtc ccaggtggga atcagcatat ctactcctca
      541 cttcagtcct aaccatcata taacttttat cttccctttt tataataaaa tgttcatcaa
      601 ttggtttaaa aaaactgggc cagccggttc cggaatcaaa tttatctgta gagctaaaaa
      661 gtggttctcc ggaaacaata tctacataga tcccctctct cttatcattc cagtactcat
      721 tctgaaaggg tttttctgtg ccgttttctt gggttacctc atattgcaac ggtgttaaaa
      781 ttctctctaa ttcttcttta gacggttttt gataatgttc atcccatttg ttttccatag
      841 catctcctga ccattttgat ttaatataat ctgctcgacc agaacctata tgatacattt
      901 ggtatcctat aggatttttt tgaaaataat cctgatgata ttcttctgcc ggataaaatt
      961 ctgctgcagg caatattttt gttacaattt cactatcaaa cctgcctgaa ttttgtaatt
     1021 cttgttttga aagttcggct gctaatttct gttcatcatt gtgataaaaa attgcagtct
     1081 gatactgact tccccggtca gcaaactgtc cgccaacatc agtagggtca atatttttcc
     1141 agaagacggc cagcaattct tcaaaagaaa ccattgccgg gtcaaagaca acttccactg
     1201 cttcgtaatg gccggttttt ccggtagata cttcttcata ggttggattg tccgtttttc
     1261 cacctgtata gccggaaatt gcctttaata ccccttcgac tttctcaaag gctgcttcta
     1321 tacaccagaa acaacctccg gcaaaagttg ccttctcggt tatggtctga ccaaaaacag
     1381 agccagtagt aaaaaatatt ataaggaata ttgtcaatat tacaatcaat atgttcttca
     1441 taatagccac tccataattt atacaatgtt gtcagtcaaa tcagttacta taaaatttaa
     1501 ctatattttt gctcccattc tcattattcc tatgttttat attataacat actcattaaa
     1561 aagtatgttt gcccttaaag ttttacactg gcaaatttta tcaccattat taattgccag
     1621 ctggctgggg ttggctttag agaaattgtt agatataagg agaacaattt gcagaagata
     1681 agattgacat gtacctttcc caatgtctgt gattataata aaaaaagaga taattaaaat
     1741 atttattaac catagggggt taaaaagaca taaataatta atagaattta accgaaataa
     1801 aataaacata caaagtaaag tttaaatgtc aaataacagc tactaaaatg tcattttaag
     1861 agatgccaag agtatgtcat acaatctccc tcatttc
//
LOCUS       DAOQWT010000340         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_72003_length_1897_cov_2.602063, whole genome shotgun sequence.
ACCESSION   DAOQWT010000340 DAOQWT010000000
VERSION     DAOQWT010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72003_length_1897_cov_2.602063"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PKJ95_06625"
     CDS             <1..575
                     /locus_tag="PKJ95_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNR65945.1"
                     /translation="EQFNLDKAEGVLVADVVPQGPAEESGLARGDIVLSVNDKAVNKT
                     QELQDAIRALQIGDKATLKVKRDGKEISFVVKIGEMPADETLTTKDKVFTEQTGLRVD
                     TVTPDIARNAGLSKVTGVVIIDVIPGSPAEDMGLQNGDIILEANRKEVSSISDWEGII
                     SKLAPGDTLLLLIFRSNRTYYVPLKIVELE"
     gene            723..>1897
                     /locus_tag="PKJ95_06630"
     CDS             723..>1897
                     /locus_tag="PKJ95_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNR65946.1"
                     /translation="MVLNRAMKKLHSRNNVYIVLIIVLLFTLLLGYISYYELQREKNY
                     LLELAHSEGLNIAFSIQTLGSEFIVSGNVLTEVLNLFQKEGTTFIDIVDRNGLVRMST
                     DKERMDKQVEIQYPGRVNYYQTRDGKNRRILQIVKPFDFGDHFSSDLFGFIFLRDKYL
                     SIGINLEGYYLRYNQIKQRVILNYLVILIISLLGVFMVFRLQENIVVKRTLRNMKDYT
                     TKLLETMDSGVISVDQHNIVQTINKKSEEIFQVKRDEVLGKNAEEVLPLKIRGKSLYE
                     LGLKRGEKIEEEIELENKAHISKNLLINTSFLEGEQNQAGGMVILLRDITRLKRLSEE
                     INQNKRLASLGQIASAIAHEIRNPLSSIRGLTQFLFQSCAEENEHKNDLKVIIKEVDR
                     "
BASE COUNT          634 a          295 c          418 g          550 t
ORIGIN      
        1 cagaacaatt taatctggat aaggctgaag gggttcttgt agctgatgtt gttccccagg
       61 gacctgctga ggagtccgga cttgcccggg gggatatagt cctatctgtt aatgacaaag
      121 ctgttaataa aacccaggaa ctgcaggatg ctatccgcgc cctgcaaatt ggcgataaag
      181 caactttgaa agtaaaaaga gatggaaaag agatttcttt tgtagtaaaa attggggaga
      241 tgccggcaga tgaaaccctg accactaaag ataaagtatt tacagaacag accggtctca
      301 gagtggatac ggtaactccg gatattgccc gtaatgccgg tttatcaaag gtgaccggtg
      361 tggttattat tgatgttatt cctggaagtc cggcagaaga tatgggttta caaaatggag
      421 atatcattct ggaggcaaac aggaaagagg tatcttctat ctcagattgg gagggtatca
      481 taagcaaatt agctcccgga gataccttgc ttctcttgat tttcagaagc aatcgcacat
      541 actatgttcc ccttaagatt gtggaattgg aataacacca cacctttacc agagaaaaga
      601 aatccaatcc tatgattggg aaataaagaa tatgcgaata ttatgataac aaatgagaag
      661 aggaaaaccg gctttatggt gctggttctc ctcttctttt aaaacagaga gaaatattat
      721 ttatggtact caacagagct atgaaaaaac tgcactcccg aaataatgtc tatattgttt
      781 taataatagt cctcttattt actttgctgc tgggctatat cagctattat gaattgcaga
      841 gggaaaagaa ttatctactg gaattagccc attcagaagg attgaatatt gctttttcta
      901 ttcagacttt aggttctgaa ttcattgtca gcgggaatgt cttaacggaa gtactaaatc
      961 tctttcaaaa agaaggtact acctttattg atatagtgga tagaaatggt ctggtgcgga
     1021 tgagcactga caaagagaga atggataagc aggttgaaat acaatatccg ggaagggtta
     1081 attattatca aacccgtgat gggaaaaaca gaagaatact tcagattgtc aaaccttttg
     1141 attttggtga ccatttctct tctgatttat tcggatttat ttttttacgg gataaatacc
     1201 tttctatcgg tataaattta gaaggttatt atcttcgtta caatcagatt aaacagagag
     1261 ttattctgaa ttatctggtt attcttatta tttctctgct gggtgttttt atggtctttc
     1321 gattacagga gaatatagta gtaaaaagaa ctcttcgtaa tatgaaggac tataccacca
     1381 agcttttaga gactatggat agtggggtaa tctctgttga ccagcataac atcgtacaga
     1441 ctattaataa gaagagtgaa gagatatttc aggtgaaaag agatgaggtg ttaggtaaaa
     1501 atgccgaaga agtgcttcct ctaaagatca gaggcaaatc actctatgag ctgggattaa
     1561 agaggggaga aaaaatagaa gaagagattg aattggaaaa caaagctcat atttcaaaaa
     1621 atctgttaat caatacttct tttctggagg gtgagcaaaa tcaggcagga gggatggtaa
     1681 ttttgttaag agacattacc agattgaaaa gattatcaga ggagataaac cagaataaaa
     1741 gactggcatc tctgggacaa atagccagtg ctattgccca tgagataaga aaccccctta
     1801 gttccatcag gggtttaact cagtttcttt ttcaatcctg tgctgaagaa aatgagcata
     1861 aaaatgattt aaaagtaatt atcaaagaag tagaccg
//
LOCUS       DAOQWT010000341         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_72358_length_1891_cov_3.983660, whole genome shotgun sequence.
ACCESSION   DAOQWT010000341 DAOQWT010000000
VERSION     DAOQWT010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72358_length_1891_cov_3.983660"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKJ95_06635"
     CDS             <1..670
                     /locus_tag="PKJ95_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNR65947.1"
                     /translation="IVSLTHFKGHELTGFGGTLKNIGMGSGSRAGKKEMHSTSKPRMK
                     EENCISCGICIENCAQGAIHYNKKKKAEIDYQKCIGCGQCVAVCQYGAAVAGSEASSV
                     IVQEKIIEYSYAVLKDKPHFHISFIMNVSPFCDCWSYNDMAIVPDIGMAASFDPVALD
                     RACVDLVNEASMVKKSILEERNYQKGEDKFHYIHPDTDWEAGLRYAEELGLGTQKYRL
                     IEVP"
     gene            744..1277
                     /locus_tag="PKJ95_06640"
     CDS             744..1277
                     /locus_tag="PKJ95_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNR65948.1"
                     /translation="MKKKISGIIIECLVGDIAIQKDIMAVVNAANAQLSTGGGVAGAL
                     HRAAGPGLEEECRPLAPIKPGEAVITGGHKLPNRYVIHCLGPIYGINSPEDILLANCY
                     RNALQLADQHRIDSIAFPAISTGIFGYPIEEAAQVTFETIKETIPQLAYVKHIRFVLY
                     DQGTLEIYERYLEKLDK"
     gene            complement(1299..>1891)
                     /locus_tag="PKJ95_06645"
     CDS             complement(1299..>1891)
                     /locus_tag="PKJ95_06645"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNR65949.1"
                     /translation="KMYQNKIFNAQSREKHLLSAFLTILAERDVHTANHAQHLTKISS
                     MMGHKIGLNSYDLNRLRLLALLHDIGKIGIPETILFKEEPLTGEEWKKLKKHCQIGYR
                     ITKNIPDFSSIAKEILYHHERWDGKGYPSGLKEEGIPLLSRIIAIVDAYDAMQSKRNY
                     KKALSTEEAREEIKRNAGSQFDPHLVNVFLEIVKPK"
BASE COUNT          576 a          318 c          391 g          606 t
ORIGIN      
        1 aatagtttct cttactcatt ttaaaggcca tgaattgacc ggttttggag gaactttgaa
       61 aaacattgga atgggaagtg gttcacgagc tggtaagaaa gagatgcact ccacttcaaa
      121 gcccagaatg aaagaagaga actgtatcag ttgtggaatt tgtattgaaa attgcgcaca
      181 gggtgccatt cattataata aaaagaaaaa agcagaaatt gattatcaaa aatgtatcgg
      241 ttgtggtcaa tgtgtggcgg tatgtcagta tggtgctgcg gtggcaggtt cagaagcttc
      301 aagtgtcatc gtgcaggaaa aaattataga atattcttat gctgttttaa aagataaacc
      361 acattttcat atcagtttta ttatgaatgt ttcccctttc tgtgactgct ggagctataa
      421 tgatatggct attgtgccag atataggaat ggctgcttct tttgatccgg ttgctctgga
      481 tagagcctgt gtagatttag tgaatgaagc ctctatggtt aagaaatcta tcctggaaga
      541 acgcaactat cagaaaggag aagataaatt tcattatata catcctgata ccgattggga
      601 agcagggttg aggtatgctg aagaattagg tttaggtact caaaaatata gattaattga
      661 agtgccataa ccacaaatat tgttgctcca aaccagtatt ttccattaat ataaataata
      721 tctatgaagg aggcggaacc gctatgaaaa agaaaatatc aggaataata attgaatgtc
      781 tggttggtga tattgctatc cagaaagata taatggcagt ggtgaatgct gccaatgctc
      841 aattgagtac aggagggggc gtggccggtg ctttgcatag agctgccgga cctggattag
      901 aagaggaatg taggccctta gctcccatca aaccaggtga ggctgttata actggcgggc
      961 ataaattacc aaatagatat gttattcact gcttgggacc catatatggt atcaacagtc
     1021 cagaggatat actgctggct aattgctatc gtaatgcctt gcagttagca gaccagcacc
     1081 gtattgactc aattgccttt ccggcaattt ctaccggtat ctttggttac ccgattgaag
     1141 aggctgccca ggttactttt gagacaataa aggaaacaat accacaactt gcctatgtaa
     1201 aacatattcg ttttgtttta tatgatcaag gtacgttgga aatttatgaa agatacctgg
     1261 agaagcttga taaatagatt ctatttaaaa ttattatttt atttgggctt gacaatttct
     1321 aagaaaacat tgaccaaatg tgggtcaaac tgacttcctg catttctctt tatttcttca
     1381 cgtgcctctt cagtgcttaa agcttttttg taatttcttt ttgattgcat tgcatcatag
     1441 gcatcaacaa ttgcaataat cctggatagc aagggtattc cctcctcctt taatcccgat
     1501 gggtaacctt tcccatccca tctttcgtgg tgatacagta tttctttagc aattgaagaa
     1561 aaatcaggaa tatttttggt aatacgataa ccaatctggc aatgtttttt tagttttttc
     1621 cattcctctc cagtaagagg ttcttctttg aatagaatag tttcaggaat accaatctta
     1681 ccgatatcgt gtaacagagc taataaacga agtctattta aatcatagct atttaaaccg
     1741 attttgtgac ccatcataga agatattttt gttaaatgtt gagcatgatt tgctgtatgc
     1801 acatctcttt ctgccagtat ggtaagaaaa gcactcagca ggtgtttttc ccgactttga
     1861 gcattgaata tcttattttg atacatctta t
//
LOCUS       DAOQWT010000342         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_72574_length_1888_cov_3.097109, whole genome shotgun sequence.
ACCESSION   DAOQWT010000342 DAOQWT010000000
VERSION     DAOQWT010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72574_length_1888_cov_3.097109"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PKJ95_06650"
     CDS             complement(<1..287)
                     /locus_tag="PKJ95_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc/iron permease"
                     /protein_id="HNR65950.1"
                     /translation="MELSVFSWIVIFGLLALIVNSIGIFIVYKNKELAEKANTYFMCF
                     AAGLLITVPLVLVFPEAVEKNSAAGSAALVGFLFMFLSNRIIQHKTRQTTLA"
     gene            1002..>1888
                     /locus_tag="PKJ95_06655"
     CDS             1002..>1888
                     /locus_tag="PKJ95_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNR65951.1"
                     /translation="MKKNKFLRIFMLIMVFSLTLGLSILATISSSRANTDSPIVGASP
                     QKVEDRFVAEPGEYIVESWVENLHIPWELIFLSDEKALVTERAGSVRLIENGVLQEKP
                     YKIIDEVEHIGEGGLMGLTKHPDYPAQKYLYVMYTYRDNRALYNRVARYIDSGDSMEF
                     DRLIIKKIPASNVHNGGRIAFGPDGLLYITTGDTWHSEIAQNIDILGGKILRLTADGD
                     MPADNPFPGSPVYSFGHRNPQGLAWHPETGVLFSSEHGPTGEFGLYSRDEVNIIEKGG
                     NYGWPLVVGAAGVESYKDP"
BASE COUNT          539 a          358 c          393 g          598 t
ORIGIN      
        1 gccagggttg tttgtcgggt cttatgttga ataattctat tactgagaaa catgaatagg
       61 aatccaacca gagctgcaga accggcagcg gaattttttt ccaccgcttc cgggaaaacc
      121 agtaccagcg gtactgtaat taaaaggcct gcggcaaaac acataaagta agtatttgcc
      181 ttttctgcta attctttatt cttataaaca atgaatattc ctatgctatt cacaatcagt
      241 gccagcagtc caaagatgac aatccatgaa aatacactta attccacacc aatctacctc
      301 ctttctacct gtatttacat tttaaacaat gctatgtaag aaaacaactg cctcctaatt
      361 ttatgttctt tgtggaaaga gcaatctcca gttgtaattc tgtccttggc actattttag
      421 ctcttcagaa actgataata aagtttatcg tttttggttt ttagtttttg gttacttatg
      481 tcttgaaccc gtacttctat ttaagatatc tctattattg acctattccc aatttatttt
      541 ttactatagg caaaaagttt ttactatgaa tcttttactt tcaatactcc tggtatatcc
      601 cgatgagatt gcttcgtcgc tgctctcctt gcaatgacgg aaggggtgga agcaatcttt
      661 gacagaataa ctgccccctg agataaacgg gtgagattga ccattgcagt tattagtgtt
      721 cggtttttta tttagtatat gtagaagcag tatcaatata ttattaagta ttcattagtg
      781 aatggtaaat gcagaaaaca gcgaaggccg aaagattccc aaaaaatctc ccgaccttta
      841 ccatttattt tgtatcactc acaagaaatt gttaaataca agctctgtcc aatgatgtca
      901 aaggtccgtc actggaaagg tttctctgac agggtttttg atatatggta gaattattgt
      961 ggtagcaatt gaagctgctt ttcagagtag gggtgatatt tatgaaaaaa aataaatttt
     1021 taagaatttt tatgctaatt atggtatttt cacttactct tggtttgagt atattagcaa
     1081 caattagtag cagcagggca aatactgatt ctcccattgt aggcgcttca ccacaaaagg
     1141 ttgaagaccg ttttgttgct gagcccgggg aatacatagt ggagagctgg gtagaaaatc
     1201 tgcatatccc atgggaacta atctttctgt ctgatgagaa agcccttgtt accgaaagag
     1261 ccggttcagt ccgattgata gaaaatggtg ttttacagga aaaaccttat aaaataatcg
     1321 atgaggttga acatataggt gaaggcggat tgatgggtct gaccaaacat cctgattatc
     1381 ctgcacagaa atatctttat gtaatgtaca cctaccggga taatagagca ttgtataacc
     1441 gagtggcacg ttatattgat agcggtgatt ctatggaatt tgaccggctt attatcaaaa
     1501 aaattcctgc ttccaatgta cataatggtg gacggattgc ctttgggcct gatggcctgc
     1561 tctacattac aaccggtgat acctggcatt ccgagattgc ccagaatatt gatattcttg
     1621 ggggaaagat tctccgtttg actgccgatg gagatatgcc cgctgacaac cctttccccg
     1681 gctcgcctgt ttattctttc ggtcatcgta acccacaggg actggcctgg catcctgaaa
     1741 cgggagttct tttttcttcc gaacatgggc ctaccggtga atttggctta tatagcaggg
     1801 atgaggtgaa cattattgaa aaagggggta attacggctg gcccctggtg gtgggtgctg
     1861 ccggggtaga atcgtataaa gaccccat
//
LOCUS       DAOQWT010000343         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_72575_length_1888_cov_2.978178, whole genome shotgun sequence.
ACCESSION   DAOQWT010000343 DAOQWT010000000
VERSION     DAOQWT010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72575_length_1888_cov_2.978178"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /gene="glyS"
                     /locus_tag="PKJ95_06660"
     CDS             <1..1394
                     /gene="glyS"
                     /locus_tag="PKJ95_06660"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNR65952.1"
                     /translation="DSENIDLCDSGKFDEKYLNLPVEVLSAVMIKHQKYFPIFNNDGT
                     LLPLFLVVINGNEDRFTKSIIKGNERVLKARLEDAKFFYQEDQKITQPGVKPLDRNRL
                     KLKNVIFQENLGTIYDKVERLTILSKRIAQELKISSDSNPIVILERSAQLCKSDLVTE
                     MVKEFPELQGIMGREYALLQGEDSQVATTIYEHYLPRFSGDNYPKTITGSILSIADKL
                     DNIVSCFINGLIPDGSQDPYALRRQALGIINIIILQKIHFSVDKVIDLNINLFLANRD
                     LAEQLNFKNKISIQMVKDFIFQRLRYLLLEKQYRYDIIDAVLAKNPNSIFDILNRITS
                     IQRIYHTSNFLQTITAATRASNLSKNTSNLEIDVNILQEKEEHSLYNQYIKTKEAIEK
                     IMTKQCYDEAYACLKTLNKVVNVFFDEVLVMVDDENLRNNRLALLKNIADLYFTVADL
                     SKIALAKGTIRGD"
     gene            1401..>1888
                     /locus_tag="PKJ95_06665"
     CDS             1401..>1888
                     /locus_tag="PKJ95_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNR65953.1"
                     /translation="MKKYVYYFDEGNKEMKKLLGGKGANLAEMTKMGLPIPQGFIITT
                     EACIEYTKNQKQFPEGLWEQVQDNLKKLEKNSNKIFGNIENPLLVSVRSGAAISMPGM
                     MDTILNLGLNDHTIQGIIKKSNNERFAFDSYRRFIQMFGNVVMDIKGELFERILEETK
                     QK"
BASE COUNT          718 a          272 c          327 g          571 t
ORIGIN      
        1 aagatagtga aaatatagat ctttgcgatt ctggtaaatt tgatgaaaaa tatcttaatt
       61 taccagtaga ggtattatct gcagtaatga taaagcatca aaaatatttt ccaattttta
      121 ataatgatgg tactttatta ccactatttt tagtggtcat caatggcaat gaagatagat
      181 ttacaaaatc tattataaaa ggaaacgaga gagtcttaaa agctaggtta gaagatgcca
      241 agttctttta ccaggaagat caaaaaatca cccaaccggg tgtaaaacca ttagacagga
      301 acaggttaaa attgaagaat gtcatttttc aagaaaattt aggaaccatt tatgataaag
      361 ttgaaaggtt aaccatactg tcaaaaagga ttgctcaaga attaaagata agttctgatt
      421 ccaatcccat agtgatatta gaaagatctg ctcaattatg caaatcagac cttgttaccg
      481 aaatggtaaa agaattccca gaacttcaag gaattatggg cagggagtat gcacttcttc
      541 agggagaaga ctcacaggtt gctactacca tctacgaaca ttacttaccc agattctccg
      601 gtgataatta tccaaaaact attaccggta gcatacttag cattgctgat aaattagata
      661 atatagtatc ctgttttatt aacggtttaa tacctgacgg gtctcaggat ccttatgcct
      721 taagaagaca agcattggga attatcaata taattatatt gcaaaaaata catttttctg
      781 ttgataaggt aattgattta aatataaatc tttttttagc aaacagggac ttagcagaac
      841 agcttaattt taaaaataag attagtattc aaatggtaaa agattttatc tttcaacggt
      901 tacgttacct gcttttagaa aaacaatacc gatatgatat cattgatgcg gttttagcta
      961 aaaatccgaa tagtattttt gatattttaa atcgtataac ctccattcag agaatatacc
     1021 atacctcaaa tttcctccaa acaattaccg ctgctaccag agcttctaat ctatctaaaa
     1081 acacatcaaa tttagaaatt gatgtaaata tcttacagga gaaagaagag cacagtcttt
     1141 acaatcaata tataaagaca aaagaggcaa ttgaaaaaat aatgacaaaa caatgttatg
     1201 atgaggctta tgcttgtctg aaaaccttaa ataaagtagt caatgttttt ttcgatgaag
     1261 ttttagtaat ggtagatgat gagaatttac gtaataatcg tctggcgtta ctaaaaaata
     1321 ttgctgattt gtattttact gttgctgatc taagcaaaat cgccctggca aaagggacaa
     1381 ttagaggaga ttaaaataaa atgaagaaat atgtctatta ttttgatgaa ggcaataaag
     1441 aaatgaaaaa attattgggt ggcaaaggag ctaacctggc tgaaatgact aaaatgggct
     1501 tgccaatccc ccagggattt atcattacca cagaggcttg cattgaatat actaaaaatc
     1561 aaaaacaatt cccggaaggt ttatgggaac aagtacagga taatctcaaa aaattagaaa
     1621 aaaattcaaa caaaatcttt ggtaatattg aaaacccatt attagtttcc gttcggtccg
     1681 gtgctgccat ttctatgccc ggtatgatgg ataccatttt aaatttaggt ttaaatgacc
     1741 atactattca gggaataatt aaaaaatcaa acaatgaaag atttgccttt gacagttacc
     1801 gaagatttat tcagatgttt ggaaatgtgg taatggatat aaaaggagaa ctttttgaaa
     1861 ggattttgga agagacaaaa caaaagga
//
LOCUS       DAOQWT010000344         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_72730_length_1885_cov_3.846448, whole genome shotgun sequence.
ACCESSION   DAOQWT010000344 DAOQWT010000000
VERSION     DAOQWT010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72730_length_1885_cov_3.846448"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..908
                     /locus_tag="PKJ95_06670"
     CDS             129..908
                     /locus_tag="PKJ95_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNR65954.1"
                     /translation="MNRDFFLNKDNKKNIFIILILALLFFVLCNSAQSQTLKYGSLSV
                     ESYPRGAKVYLDNNYKGTTPLNLKNIAIGQYTLKLVLEGYQDWTSTVVILPILTVTIS
                     ADLVPEKDEGFGSISVNSNPQGADIYIDGAFAGITPINIQNFSSGRYRIRVTLPGYED
                     WFDEIFVTSRKTERVNAELVPRPNYGSLSLYCDQNDAKVFLNGVYKTDIFDTPTVIED
                     LDSGNYEIVVIKEGFRAWVGDITVNVNEETSLDVIMTEIFG"
     gene            complement(962..>1885)
                     /locus_tag="PKJ95_06675"
     CDS             complement(962..>1885)
                     /locus_tag="PKJ95_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNR65955.1"
                     /translation="LVVYFWSEIWCTFRLKFTIVLDPIFIFVLGYGVKGAAIATIIGQ
                     GVALFYYFNFYLMKKSMVSLHWKYFSFRSDILLEILKVGIPASSYNIMINITNIIGIY
                     VAASYNDLIVAAYGVNRRIFSMCIMTLTGLAEGTQPLIGYSYGARKIERMNKIIKTAL
                     VMAASVSFFFVFFFYLFAGKMIQIFINNTEVITYGIQIMRAMIISLPFIGIQFLIRTN
                     FQALGKGRPALILALARDFFYIPALFTLNKYFGFSGFIYAQPFSNASTFLLALFLFNS
                     IRGQVSDEHREWKIANSVITAEATNPLEKDN"
BASE COUNT          652 a          310 c          343 g          580 t
ORIGIN      
        1 accgaaatta ctggttaagg tataataaat taaacaagat taaatagagg tgataaggac
       61 atacctgaca tggttgcttt agtttataat atctttctaa tgagttttat ttttggagga
      121 atatttaaat gaatagagat ttttttctta acaaagacaa taaaaaaaat atttttatta
      181 ttcttattct ggcacttcta ttctttgttt tatgcaattc agcacagtct caaacactaa
      241 aatatgggtc actttcagtt gagtcttatc ccagaggggc aaaggtttac ctggacaata
      301 attataaagg aaccacccct ttaaatttaa agaatattgc tatcggtcaa tacactctta
      361 aactggttct ggagggctac caggattgga ccagcacagt ggtaatttta cccattctaa
      421 cggtaactat ttcggcagat ctggtccctg aaaaggacga gggttttggt tcaatctcag
      481 ttaattcaaa tccacaagga gctgatattt atattgatgg tgcctttgca gggataacac
      541 caataaatat acagaacttt tcttccggta gatatagaat tagagtaact ttacccggtt
      601 atgaagactg gtttgacgag atatttgtta cttcacgaaa gacagaaagg gtaaatgctg
      661 aattggtgcc tcggcccaat tacggctcat tatcgctcta ttgtgatcag aatgatgcta
      721 aagtatttct aaatggtgtt tataaaacag atatttttga cactcccact gtcatagagg
      781 atttagatag tggaaattat gagatagtgg taatcaaaga gggattcaga gcctgggttg
      841 gagatataac ggtcaatgtt aatgaggaaa catctcttga tgttattatg accgagattt
      901 ttggataaac ataaccttaa catatcttaa tatatatagt agaatttgta agctttagta
      961 tttaattatc tttttctaaa ggattggtgg cttcagcagt aatgacactg tttgcaattt
     1021 tccattccct gtgctcatcg cttacctgtc ctctaattga gttaaagagg aaaagcgcca
     1081 gtaaaaaggt agaggcatta ctgaatggtt gagcataaat aaatccagaa aacccaaaat
     1141 atttatttag ggtaaaaaga gcaggaatgt agaaaaaatc acgggccagg gctaaaatca
     1201 aagcaggcct tcctttgcct aaagcctgga agttggttct tattaaaaat tgaattccaa
     1261 taaagggtag agatataatc atagctcgca taatctgaat accatatgtg attacctctg
     1321 tattgttgat gaagatttga atcatctttc cggcaaagag atagaaaaag aagacaaaga
     1381 agaaagaaac actggcagcc ataaccaggg ctgttttaat aattttattc attcgttcaa
     1441 tttttctggc accataacta tatccaatta aaggttgtgt gccttctgcc aatccagtta
     1501 aagtcatgat acacatggaa aatatacggc gattaacacc ataagcagct actatcaaat
     1561 cattataact ggcagcaacg taaataccta taatattggt gatgtttatc attatattgt
     1621 atgatgaagc aggtatccct actttcagga tctccaataa aatatcactt cgaaaagaaa
     1681 aatatttcca gtgaagagaa accatacttt tcttcataag gtaaaaatta aaataataga
     1741 agagagcaac tccctgtccg attatggtag caatagcagc tccttttact ccatatccca
     1801 ggacaaatat gaagatagga tctaaaacta ttgtaaattt caaccgaaaa gtgcaccata
     1861 tttcactcca aaagtacacc actaa
//
LOCUS       DAOQWT010000345         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_73096_length_1880_cov_2.933151, whole genome shotgun sequence.
ACCESSION   DAOQWT010000345 DAOQWT010000000
VERSION     DAOQWT010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73096_length_1880_cov_2.933151"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /gene="yqeC"
                     /locus_tag="PKJ95_06680"
     CDS             complement(<1..463)
                     /gene="yqeC"
                     /locus_tag="PKJ95_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HNR65956.1"
                     /translation="MLISDALQLKERAFLSLVGAGGKTSLLQIIAQELVGKKKKVIVT
                     TTTRMFTRQASPLLGAGQIIESTNARSMEDGIKDYFGVKRDNGIAILFKDRFLEVGGE
                     KFSGPEPYYLNKWWQEGLADFFIVEADGAKGRPIKAPSQHEPVVASMTTDVV"
     gene            complement(663..1139)
                     /locus_tag="PKJ95_06685"
     CDS             complement(663..1139)
                     /locus_tag="PKJ95_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNR65957.1"
                     /translation="MDNKINIKVNINGDDYSLEVEPNTLLLDLLRNKLGLTGTKLACG
                     TGECGACTVIMDGLTVNSCLVLAAEVDGKNITTIEGLSKNGELHPLQESFIEEGAVQC
                     GFCTPGMILSSKALLDKNPNPTDEEIKIAISGNLCRCTGYKKILKAIRSAAQKMAC"
     gene            complement(1154..>1880)
                     /locus_tag="PKJ95_06690"
     CDS             complement(1154..>1880)
                     /locus_tag="PKJ95_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNR65958.1"
                     /translation="EYIIDIKNVPELNGITYSPEGELRIGALVTHRQFAISKNVPEKF
                     TMLKEASLAVGSVQTRNRGTIVGNICTSSPAADTPPALLALDAGLKLVSSTGERTVKI
                     CDFFTGPFKNILKDTEILTEILIPDLPENSGGIYLWHSKITEEDETLVGVGVVVSVDN
                     LQNKVCKQARIGLGSVAPAPMRAVKTEDFLKGKILDEDTIGQAAEIAANESLPRSRAE
                     YRKEMVKVQTKRALSQALKRIEG"
BASE COUNT          532 a          448 c          279 g          621 t
ORIGIN      
        1 ccactacatc tgtagtcatg gacgccacca ccggctcatg ttgagaggga gccttgattg
       61 gtcttccctt tgcgccatcg gcttctacaa taaaaaaatc tgccagtcct tcctgccacc
      121 atttattaag ataataaggt tccggacctg aaaacttttc ccctcccact tccagaaacc
      181 tatctttaaa aagtatggct attccgttat ctctcttcac tccaaaataa tctttaatac
      241 catcttccat tgacctggca ttggtagatt ctataatctg ccccgctccc aataaaggag
      301 aggcctgacg ggtaaacatc ctggtagtgg ttgttactat aacttttttc ttttttccta
      361 ccaattcctg agctattatt tgtaaaagag aggttttacc tccagcacca accagagaca
      421 gaaaagctct ttcttttaat tgcagggcat cgcttatcag catctgatta acttcctgaa
      481 gttttattaa ttttacacca atactaaaaa taatgaatca catctatccc cttttttata
      541 tatgtgattc attattataa aaatattcta ctgtcttaac agtctataat attaaaattt
      601 tagttagcca cgcaaaatag aaacaggaat aaaccctttc caaaattctt caacactatc
      661 gtttaacagg ccatcttctg tgcagcagat cttattgctt taagtatttt tttataaccg
      721 gtacatctgc ataaattgcc cgatatagct atcttaatct cttcatcggt gggattagga
      781 ttcttatcca aaagagcctt ggaagaaagt atcatacccg gggtacaaaa accacattgt
      841 actgcacctt cctctataaa gctctcctga agtggatgga gttcgccatt cttagacaat
      901 ccctcaatag tagtaatatt tttaccatca acttctgctg ccaataccag gcatgaattg
      961 acagtcaatc catccataat cacggtacag gctccacact ctccggttcc acaggccagt
     1021 ttggtaccag tcagacctaa tttatttctt agcaaatcta atagtagagt atttggttct
     1081 acctcaaggc tataatcatc accatttata tttaccttta tatttatctt attatccata
     1141 attatctcct ttattacccc tctattctct ttagagcctg gctcaaggca cgctttgtct
     1201 gaactttcac catctcttta cgatattctg cccgtgaacg tggcaaactt tcatttgcag
     1261 ctatttctgc tgcctgtcca atagtgtcct catccaatat tttacctttc agaaaatctt
     1321 ctgttttcac tgctctcatt ggtgcaggag ctacagaacc taatccaatt cttgcctgtt
     1381 tacagacctt attctgtaag ttatcaacac tcactaccac tcctaccccc actaaggttt
     1441 catcttcctc tgttatttta gaatgccata aatatattcc accactattt tcaggtaaat
     1501 ctggtattag aatctcagta agtatctctg tatctttgag tatattttta aaaggaccgg
     1561 taaaaaagtc acaaattttt acagtccttt cacctgtaga actcaccaat tttaatcctg
     1621 catctaaagc cagcagagca ggaggtgtgt ctgcagcagg agatgaggta cagatattac
     1681 caacaatggt cccacgatta cgagtctgaa ctgaacctac tgctagagat gcctctttaa
     1741 gcatagtgaa cttttccggt acatttttag aaatagcgaa ctgcctatgt gttaccaggg
     1801 cacctattct tagctctccc tccggactat aggtaatgcc atttaactct ggaacattct
     1861 ttatatcaat gatatactca
//
LOCUS       DAOQWT010000346         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_73100_length_1880_cov_2.754521, whole genome shotgun sequence.
ACCESSION   DAOQWT010000346 DAOQWT010000000
VERSION     DAOQWT010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73100_length_1880_cov_2.754521"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="PKJ95_06695"
     CDS             complement(<1..1165)
                     /locus_tag="PKJ95_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNR65959.1"
                     /translation="MEDYISLYEKLGLFYLGKEIDPKTLTFKTDEAPFLYKSKDFTTH
                     AAIIGMTGSGKTGLGIAIIEEAAIDKIPSIIIDPKGDMGNLLLAFPELKPSDFQRWID
                     PLEAESKGMKPDVYAEEVAGNWEKGLRGFHQDKSRISLYSNSADFTIYTPGSMAGIPI
                     SVLSSFEAPSEDVMNDPDTYSAMINSTVMGLLSLLNVRADVLTSKEYILLAALFSYFW
                     KKNINLTLEQLIGYITNPPFEKIGVLSLNSFYPQKERFNLAMLLNNILSSPGFSTWLE
                     GKNLDIQDLLYTENGKPRVSILSLAHLDSNQQMFFVTLFLNKYISWMRQQTGASSLRT
                     LLYMDEIFGFFPATSNPPSKQPMLILLKQARAYGIGVVLATQNPVEHNGTYPLP"
     gene            1465..>1880
                     /locus_tag="PKJ95_06700"
     CDS             1465..>1880
                     /locus_tag="PKJ95_06700"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HNR65960.1"
                     /translation="MYNRKKTKISSILINGLLVTLFLLLLTGVAFSTEEAPQILITSI
                     GQSPDARMINVLLSRYQISAAYEQLATPEMVKNFKMIIAVVGGSSKGLGAAGIDKEQE
                     LSRSKALIKEVKELKVNLLVMHIGGEARRGALSDAF"
BASE COUNT          605 a          329 c          354 g          592 t
ORIGIN      
        1 ccggcagcgg gtaggtaccg ttgtgctcca caggattctg agtggccagc accactccta
       61 taccataagc tctggcctgt tttaacagaa tcagcatagg ctgtttggaa ggtggattgg
      121 aagttgctgg gaaaaaacca aaaatctcat ccatatagag taaggttcga agtgaagagg
      181 ctcccgtttg ttgtctcatc cagctgatat atttattcaa aaagagggtc acaaaaaaca
      241 tctgttgatt agaatctaaa tgggctaggg aaagaatact gactctgggt tttccgtttt
      301 cagtgtataa aagatcttga atatctaaat ttttcccctc taaccaggta gaaaaaccgg
      361 ggctggacag aatgttattt aaaagcatag ccaggttaaa tctttccttt tgtggataaa
      421 aactatttag acttaagaca cctatcttct caaaaggagg attggtaata taaccaatta
      481 attgttctaa ggtaagatta atatttttct tccagaaata ggagaacaat gctgctaaga
      541 gaatatattc tttactggtt aggacatctg ctctgacatt gagcagggaa agaagtccca
      601 ttaccgttga attaatcata gcagaatagg tatccggatc attcataaca tcttcagaag
      661 gtgcttcaaa actgcttaat actgatatag gaatacccgc catactaccg ggtgtataga
      721 tggtaaaatc tgcactatta ctataaaggc taattctgct cttatcctgg tgaaagcctc
      781 ttaacccttt ctcccagtta cctgctacct cctcagcata gacatcgggc ttcataccct
      841 tgctctcggc ttctaaggga tctatccacc gttgaaaatc tgacggcttt aattctggaa
      901 aagcaagaag aaggttaccc atatctcctt tgggatcaat tataatggaa ggaattttat
      961 caatggcggc ttcttcaatt atagctattc ccaatccggt tttgccactg ccggtcatac
     1021 cgattatggc agcatgtgta gtaaaatctt tggatttata aagaaaggga gcctcatcag
     1081 ttttaaatgt taaagtttta ggatcaatct cttttcctaa ataaaacaaa cctaattttt
     1141 catataaaga aatatagtct tccatttttt cctcctcacc ctattttatg gggacgattc
     1201 tattgacaaa atttaataaa tgaatttaat atttgtcagt aaaactgtct tatgtttttt
     1261 attatatcag taaatatctt acctgtatat tataacaaat ttattagaat aagtgttttg
     1321 tttatgtatt ttgttgctta tgatggacaa ctgatctcga taatcttaca attaccctaa
     1381 ttttgtgata agatataaaa aaacagcact taatttatta ctcaaagact tggaataggc
     1441 aatattaata aggaggcatt aataatgtat aatagaaaaa aaactaaaat aagcagtata
     1501 ttaataaatg gattattagt aacactcttt ctgctccttt taacaggagt tgcgttttca
     1561 acagaggagg cgcctcaaat attaataact tcaattggtc agagtccgga tgccagaatg
     1621 ataaatgtat tattatcacg ataccagatt tcagctgcct atgaacagct tgccacacct
     1681 gaaatggtaa aaaatttcaa gatgattatc gcagtagttg gtggtagttc taaaggactg
     1741 ggggccgctg gaatagataa agaacaggaa ttatccagat caaaagctct gattaaggag
     1801 gtcaaagaac tgaaagttaa ccttctggta atgcatattg gtggagaagc tcggaggggt
     1861 gccctttctg atgctttttt
//
LOCUS       DAOQWT010000347         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_73376_length_1876_cov_2.491488, whole genome shotgun sequence.
ACCESSION   DAOQWT010000347 DAOQWT010000000
VERSION     DAOQWT010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73376_length_1876_cov_2.491488"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1876)
                     /gene="dnaE"
                     /locus_tag="PKJ95_06705"
     CDS             complement(<1..>1876)
                     /gene="dnaE"
                     /locus_tag="PKJ95_06705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNR65961.1"
                     /translation="LTDFSLPDKLTDNDYLKQLCYEGAKVQFKTLQGEVKERLDYELS
                     IIQKMGFATYFLIVWDFVDYAKKHHIMVGPGRGSAAGSLVAYCLNITNINPLDYHLLF
                     ERFLNPERISMPDFDIDFSIEGRGEVIEYVSQKYGSDKVAQIITFGTMAARAVIRDVG
                     RALGIPYGQVDKIAKLIPFEPKMTIEKALQIEPELKNRMENDAIIKKMIEISSKLEGL
                     SRHASTHAAGVVLSRESLTDFVPLQLTSEGEISTQYVAEDLESLGLLKMDFLGLRTLS
                     VINNTIKIIKKTRDVTIDIDRIPLDDEQVYQLLARGECCGLFQLESSGMTDLVKRMEP
                     KNIEDITALLALFRPGPLGSGMIDDFINRKKGKVEIQYLHPKLEPILKDTYGVIVYQE
                     QVMQIASQLAGYSLGQADILRKAMGKKIKEVMDKQQKQFIEGALKNGIDKKTADDIYD
                     LIAYFAGYGFNKSHSVSYAFLSYQTAYLKAHYPVEYMAALLTSIMQFTDKVVKYIKEC
                     QNMGLKILPPDINESLIDFTVVDRKTIRFGLAAVKNVGRAAVENIIMERKKNGPFSSL
                     IDFCHRVDLKTMNRRVMESLIKCGAFDSLKTSRAQLLSILEKSIQIAQDRQREKRNGQ
                     V"
BASE COUNT          533 a          413 c          313 g          617 t
ORIGIN      
        1 ttacctgtcc atttctcttt tctctttgtc tgtcctgggc tatctgtatt gacttttcta
       61 aaatagatag caattgagcc ctggaagttt tcagcgaatc gaaggcaccg cacttaatta
      121 agctctccat aactcttcta ttcatagttt ttaaatctac cctatggcag aaatcgatga
      181 gggaggagaa tggtccattt ttctttcttt ccataataat gttttccact gccgccctgc
      241 ccacattttt aactgctgcc agaccaaaac gtatggtttt tctatcaact acagtaaaat
      301 caatcagact ctcattgata tccggaggca gaatttttaa tcccatattc tggcattctt
      361 ttatatactt aactacttta tccgtaaatt gcataatact ggtgagaagt gcagccatat
      421 attctaccgg ataatgtgct ttaagatagg cggtctggta ggacaagaaa gcataggata
      481 cactgtgtga tttattaaaa ccatagccgg caaaataagc aatgaggtca tatatatcat
      541 cagcagtctt tttatcaata ccatttttca gggctccttc aataaactgt ttttgctgct
      601 tatccattac ttctttaatc tttttgccca ttgcttttct taaaatatct gcctgcccca
      661 gggaataacc ggccagttga ctggcaatct gcataacctg ttcctggtaa acaattaccc
      721 cataggtatc cttcaggatt ggttccagtt tgggatgtaa gtactgaatt tccactttcc
      781 cctttttacg gtttataaaa tcatcaatca taccactacc taaaggtcct ggtctgaata
      841 aggccaaaag ggcagtaata tcttcaatgt ttttgggttc cattcgtttc accagatcag
      901 tcatcccgga actttccagt tggaataggc cacagcactc tcctctggct aacagctggt
      961 aaacctgctc gtcatctaag ggtatccggt caatatctat tgtcacatcc ctggtttttt
     1021 taataatttt tatggtatta ttgattacgg aaagagttct taaacctaaa aaatccatct
     1081 tcaacaatcc cagagattct aaatcttctg caacatattg agtagaaatt tctccttcgg
     1141 aggttaactg aagtggaaca aaatcagtca atgattcacg agataagact actcctgcgg
     1201 catgagttga ggcatgacgg gacaaacctt ccaatttaga agatatttca atcatcttct
     1261 ttataatagc atcattttcc attctatttt ttaattcagg ttcaatctgc aatgctttct
     1321 ctatggtcat tttaggttcg aaaggaatga gtttggcaat tttatccacc tgtccatagg
     1381 gaatacccaa tgcccttcct acatcccgga ttactgcccg ggcagccata gtgccaaaag
     1441 taataatttg ggcgacttta tcactaccat atttttgact gacatactca attacctcac
     1501 ctcttccctc gatgctaaaa tcaatatcga aatcaggcat actgattctt tccggattga
     1561 gaaatctttc gaagagcaga tggtaatcta aagggtttat attggtaata ttaagacaat
     1621 atgccaccag actgccagct gctgagcctc tccccggccc aaccataata tggtgttttt
     1681 tggcataatc tacaaaatcc catactatta aaaaataggt ggcaaaaccc attttttgta
     1741 tgatagacaa ctcatagtcc agacgttcct taacttcccc ctgcagggtt ttaaattgta
     1801 cttttgcccc ttcataacat aactgtttta aataatcatt gtcagtaagt ttatcgggca
     1861 gactgaaatc agtcag
//
LOCUS       DAOQWT010000348         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_73941_length_1867_cov_3.097682, whole genome shotgun sequence.
ACCESSION   DAOQWT010000348 DAOQWT010000000
VERSION     DAOQWT010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73941_length_1867_cov_3.097682"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="PKJ95_06710"
     CDS             complement(<1..1413)
                     /locus_tag="PKJ95_06710"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNR65962.1"
                     /translation="MKLSTLIQMLPVEKIKGNMEIEIKGLAQNSKLVQPGDLFVCIAG
                     FVDDGHHYIPEAISRGAIAIVVQKAISINADIATITVKDTRKALAFLANRFYHYPSQG
                     LKLIGITGTNGKTTVAYILKTILEEARKKTGLLTTVENIVNGHSTISKMTTIESLDLQ
                     KLLYEMVGCQTEYAIMEVSSHALSLSRVEGCDFDIVIFTNISDEHFEIHNNFHNYLES
                     KKRLFLSLNESCKDVSNKKGIVNIDEPYACEFEKSLKGGIGLLTYGIKEKGMFRARNI
                     VLNLKKVNFIAETPVGEVEILLNMGGIYNVYNALAAIAAAVSQNISLDTIASAFKKFA
                     GAPGRYKILDYGQPYTIIIDFAHNFHGLEHILKSLKEHSKNRLITIFGHGGERDNRVR
                     KKMGQVVGRYSDYSIITADNPRSEDPAEISKEIERGFQDVENNCYSIILDRVEAIRHA
                     LEIARENDIVLIAGKGPEKEQ"
     gene            complement(1502..>1867)
                     /locus_tag="PKJ95_06715"
     CDS             complement(1502..>1867)
                     /locus_tag="PKJ95_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNR65963.1"
                     /translation="IKQHTPHTVRQGISSSTAKTMSQIMREVVENGTGTRAKLRHYSA
                     AGKTGTSQKFDFSLGRYSNEKFTSWFTGFAPSENAEIAIVVMLDEPRGSYYGGTVAAP
                     VFQEIASKVLPYLSIPPNK"
BASE COUNT          527 a          396 c          326 g          618 t
ORIGIN      
        1 ctgttctttt tccggacctt tccccgctat caatacgatg tcattttcgc gagcaatctc
       61 aagggcatgt ctgattgctt caaccctgtc taaaataata ctgtagcagt tattttctac
      121 atcctgaaat cctctttcta tttctttgga aatttctgcc ggatcttcgc tgcgaggatt
      181 gtcagcggta ataatactat aatcactgta gcgacccact acctgaccca ttttctttct
      241 tacccggtta tctctttccc caccatggcc aaaaatggta attaatctgt ttttagagtg
      301 ctcttttaat gattttaaaa tatgctccag accatgaaaa ttatgggcaa aatctatgat
      361 aatagtatat ggctgaccat aatccagtat tttataccga ccgggggcac cggcgaactt
      421 tttaaaagcg ctggcaatgg tatctaaaga gatattctga cttactgccg cagcaattgc
      481 ggcaagggca ttgtagacat tatagatacc gcccatattc agtaaaattt caacttctcc
      541 cacaggagtt tcagcaataa agttaacttt tttaagattc aagactatgt tacgggccct
      601 aaacattcct ttttctttga ttccataggt taaaaggcca ataccgcctt taaggctttt
      661 ctcaaactca caggcataag gctcatctat attgacaata ccttttttgt tggatacatc
      721 tttgcaggat tcattcaaag acaggaataa tctcttttta ctttctaaat aattgtggaa
      781 attattatga atttcaaaat gttcgtcact aatattggta aaaatgacta tatcaaaatc
      841 acagccttct acccgggata ggctcaaggc atgtgatgaa acttccataa tagcatattc
      901 agtctggcag cccaccatct cataaagaag tttttgcaga tctaaagatt ctatggtagt
      961 catctttgaa atggtagaat gtccgttaac aatattttca actgtggtca gaagacctgt
     1021 ttttttccgg gcttcttcta aaatggtttt aagtatatag gcaactgtgg tcttaccatt
     1081 agtcccggta ataccaatta atttcagccc ttgtgaaggg taatggtaaa agcggttagc
     1141 cagaaaagcc aatgcctttc tggtatcctt aactgttatg gtggcaatat cggcatttat
     1201 ggaaattgct ttttgcacta caatggctat agctcccctt gaaattgcct caggaatata
     1261 gtgatgccca tcatccacaa acccggcaat gcagacaaac aaatcaccgg gctgcactaa
     1321 tttggaattt tgagccaggc cctttatttc aatttccata tttcctttta tcttttctac
     1381 tggcaacatc tggattaaag ttgataattt catagccgat ttacaccttt gaccttgttg
     1441 tttccctgac ctgaaatatc ttacttgata ttttaaaaaa agtaaactgt tctctctact
     1501 attatttatt cggaggaata gacagatatg gtaatacttt tgaagcaatt tcctgaaaaa
     1561 ctggcgccgc caccgttccg ccgtaatagc tacctctggg ctcatccagc attactacaa
     1621 tagcaatttc agcattttct gatggtgcaa aaccggtaaa ccaggaagta aatttttcat
     1681 ttgaatatct gcccagagaa aaatcaaact tttgtgatgt tccggtcttt cctgctgcag
     1741 aatagtgcct taactttgct ctggtaccgg taccattttc cactacttcc cgcattatct
     1801 gggacattgt ctttgcagta gacgaagaaa taccctgtct tacagtatgg ggagtgtgtt
     1861 gtttaat
//
LOCUS       DAOQWT010000349         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_74229_length_1863_cov_3.128872, whole genome shotgun sequence.
ACCESSION   DAOQWT010000349 DAOQWT010000000
VERSION     DAOQWT010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74229_length_1863_cov_3.128872"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..447
                     /locus_tag="PKJ95_06720"
     CDS             25..447
                     /locus_tag="PKJ95_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNR65964.1"
                     /translation="MEDNNQFPLKAQRLSFSFFILSIILISLLVIGYICSNIILMKLG
                     YQSIDLEKEKEELLVEKCQLEYTVEKLSSLTRIESIAVQELGMCRPERIEFIAMLPSN
                     VTFDMMAKQTSVSEPEIGFMEAGTFFSEFANLKIFQNQ"
     gene            480..>1863
                     /locus_tag="PKJ95_06725"
     CDS             480..>1863
                     /locus_tag="PKJ95_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNR65965.1"
                     /translation="MEHFSEKRERILFLLFVFIFIFLLLIYRLYNIQVVGGSVLHELA
                     RKEHITSYAIDGKRGLIYDRNLKRLAVNVESKSLFAIPYKIEQPEQTARLLSSKLGLD
                     YQEIVKQLIKEKYFVWVARKLSQDKYEELKSLELDGLDFVTENKRYYPKESLASHLIG
                     FVGIDNQGLEGLELYFDKELKSQSGLVVMERDASGAKIPLSIERVQPSQNGLSIALTI
                     DEVVQYITEEALNKAFLEYRPKSAVAIVVIPKTGEILSIAVRPSFDINNYQDSSEDCR
                     RNRAITDNFEPGSTFKIFTIATALENGYARLTDNFYCKGWINYQGTVIRDIDPHGYQN
                     LTDIVKNSCNVGVIEVGTRMDKKIFAESITNFGFGEKTGIDLLGESPGLFRPVSKWSK
                     ISNASLSIGQEISVTPLQLVMGVAAIANDGVLMKPLIVTQILDEEDSIIKQYTPQTVR
                     QVISSSTAKTM"
BASE COUNT          604 a          322 c          381 g          556 t
ORIGIN      
        1 tgaaaaatga aaagaacaat tttagtggag gacaataacc aatttccttt aaaagcccag
       61 cggctatcat tctctttttt tattttatcc attattttaa taagtttact ggttatcggc
      121 tatatttgca gtaatattat cttgatgaaa ttgggctatc aatcaattga tctggagaag
      181 gaaaaggaag aacttctggt agaaaagtgt cagctggagt ataccgtaga aaaactctcc
      241 tcgttaaccc gaattgaaag catagctgtt caagaactgg gcatgtgtcg accggaaaga
      301 atagaattta ttgccatgct tcccagtaat gttacttttg atatgatggc aaagcaaact
      361 tctgtatcag agccggaaat cggttttatg gaagccggta ctttcttcag tgaatttgct
      421 aatttaaaga ttttccagaa tcagtaaaga actataaata atgggtgagg cgttttgttt
      481 tggagcattt ctcagaaaaa agagaaagaa ttcttttttt attattcgta tttattttta
      541 tctttttatt gctcatctac cgactctaca atatacaggt agtgggaggt agtgttttac
      601 atgaacttgc ccgcaaagaa catattacct catatgccat agacggtaaa agagggttga
      661 tttatgaccg aaatcttaaa agattagcag taaatgttga gtcaaaatca ctctttgcca
      721 taccctataa aattgaacag ccagaacaga ccgcccgctt gctttcctct aaactagggt
      781 tagactatca ggaaattgta aaacaattaa tcaaagaaaa atatttcgtc tgggttgccc
      841 gtaagctttc tcaggataag tatgaagaac taaaaagcct ggaattagac ggtctggatt
      901 ttgtaactga aaataaaaga tattatccca aagaatctct tgcctcacat ttaattggtt
      961 ttgtagggat agataatcag ggactggaag ggctggagtt atactttgat aaggaattaa
     1021 agagtcagtc cggtctggtg gtaatggaaa gagatgcatc aggtgccaag attcctctca
     1081 gcatagaaag ggtgcaacca tcacaaaatg gactctctat tgcacttacc atagatgagg
     1141 tagtgcaata catcacagaa gaggcgctta acaaagcctt tttagaatac cgccccaaat
     1201 cagcagtagc aatagtggta atccctaaaa caggtgaaat tttaagcata gcagtcagac
     1261 cttcctttga tattaataac tatcaagatt cttcagagga ttgccgtaga aaccgggcaa
     1321 ttaccgataa ttttgaacca ggctccactt ttaagatatt tactattgcc accgccctgg
     1381 aaaatggcta tgccaggcta actgataatt tttactgcaa aggttggatt aattatcagg
     1441 gaactgtgat tcgcgatatt gatccacatg gttatcagaa tctgactgat attgtaaaga
     1501 attcctgtaa tgtaggggtg attgaagtcg gtacccgtat ggataagaaa atatttgccg
     1561 agtccataac taattttgga tttggtgaaa agacgggaat agatcttctg ggagaatctc
     1621 ctggtctatt caggccggta agtaagtggt ctaaaatatc caatgcctct ttatctattg
     1681 gacaggaaat atcagtaacc ccccttcagc tggtaatggg agtggcagca atcgccaatg
     1741 atggtgtttt gatgaaaccg ttaatagtta cccaaatcct agatgaagag gacagtatta
     1801 ttaaacaata cactccccag actgtaagac aggttatttc ttcttctact gcaaagacaa
     1861 tgt
//
LOCUS       DAOQWT010000350         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_74685_length_1856_cov_2.818434, whole genome shotgun sequence.
ACCESSION   DAOQWT010000350 DAOQWT010000000
VERSION     DAOQWT010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74685_length_1856_cov_2.818434"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PKJ95_06730"
     CDS             complement(<1..448)
                     /locus_tag="PKJ95_06730"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65966.1"
                     /translation="MFNQKSKKIGKEVTKKRLNDLFILITLCFVVILINLWYLQIIKG
                     KEFEQRAINNCIRSLVEEAPRGEVYDEQGRLLVTNRPAVNFSIIPAEVSDYKSLVDDL
                     STIVPIDSSNIVEKIRSKRLNPFQALTIKRDLEKDKIVLIEEQKYKF"
     gene            complement(452..946)
                     /gene="mreD"
                     /locus_tag="PKJ95_06735"
     CDS             complement(452..946)
                     /gene="mreD"
                     /locus_tag="PKJ95_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNR65967.1"
                     /translation="MKIWIKAGIMIFTIILELLLGNILAFYTVKPDLILIVVICLSFL
                     SSSQEGAIIGFAGGLLKDIFSVSLLGTHALVKTAIGYLSGIIRERIFYQHLLWLIAIT
                     TFIFTFLNNTFLYYLLKSLYTEYTFLTILKKYIVIQASINCILAPFIFISIKKILAYI
                     SRWS"
     gene            complement(1249..>1856)
                     /locus_tag="PKJ95_06740"
     CDS             complement(1249..>1856)
                     /locus_tag="PKJ95_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNR65968.1"
                     /translation="KKADMVTPDGAGILWATAFLEEPFSERITGIDMVKHICQLAVEK
                     EYKLYLLGAKAEVIEKTVHNLKKQFPGIKIVGYHHGYFNNSNSETNGEKGESDIINDI
                     VMQKPDILLVGMGVPKQETWIFQNKNRLGVPVCIGVGGSFDVLSGKIPRAPLWMQNHG
                     MEWFFRLIKEPKRVKRTIYLPYFIWLVLLAKIELLFRTERN"
BASE COUNT          623 a          313 c          243 g          677 t
ORIGIN      
        1 taaatttata cttttgttct tcaataagga ctattttatc cttttccaga tctctcttta
       61 tagttaaggc ctggaaagga tttagccttt tacttcgaat tttttcaacg atattagagg
      121 aatcaatagg cacaattgtg ctaagatcat ctacgagact tttataatcg cttacttcgg
      181 caggaattat ggaaaaattt actgccggtc tattagttac taataatcta ccttgttcat
      241 cataaacttc tcctcgaggc gcttcctcaa cgagtgaacg aatgcaatta tttattgctc
      301 tttgttcaaa ttctttgcct ttaattatct gcaaatacca gagattaatt aaaataacaa
      361 caaaacatag ggttattagt ataaacaaat catttaatct ttttttggta acttctttgc
      421 ctattttttt acttttttgg ttaaacataa cttaactcca acgtgagata taggccaata
      481 tttttttgat gctgataaaa ataaaggggg ctaatataca attaatggag gcctgtatca
      541 ctatatactt ttttaatatg gtcaaaaagg tgtattcagt atataaggat ttcaacaaat
      601 agtataaaaa agtattattc aaaaaagtga agatgaaagt ggttatggca attaaccata
      661 ataagtgttg gtagaatatc ctttccctta ttattcctga tagataacca atcgcagtct
      721 ttactaaggc atgggttccc aacaaactca ccgaaaaaat atcttttaac agccctcctg
      781 caaaaccgat tatagcacct tcctgggaac ttgataaaaa agataaacaa attactacta
      841 taagtatcaa atcaggtttt acagtataaa aagccaggat atttcccagt aataattcta
      901 agattatggt aaaaatcatt attcccgctt ttatccatat cttcattttt taataacctt
      961 ttctaaataa taatcagata tatttcttta gtttattata caatcagaga attagaaatg
     1021 ttaatatatt tagataatcg ataatacaaa attttataac tatagcaatt ttaatggtta
     1081 atgtttcttt cttattatta tatgtgggaa taaaggtact caataattaa gtgttgttaa
     1141 tcagagtttt gtgggttaaa aatagatata attacatgtt tttatctttt ataaatattt
     1201 gaataaaaat attcgtttaa aagaattcct aatttatcaa ttaataatct aatttctttc
     1261 tgttcgaaac aacaattcaa ttttggccaa taagacaagc cagataaagt atggtaaata
     1321 aattgttctt tttactcttt taggttcttt aattaatcga aaaaaccatt ccataccatg
     1381 attttgcatc cataaaggag ctcggggtat tttaccggaa agaacatcaa aacttccccc
     1441 aacaccaata caaactggca ctcccaatct atttttattt tgaaatatcc atgtttcctg
     1501 tttgggcact cccattccta ccaacaaaat atctggtttt tgcatcacaa tatcattgat
     1561 aatatcgctt tctccttttt caccgtttgt ttctgaattg ctattattaa aataaccgtg
     1621 atgatagcca acaattttaa tccccggaaa ttgctttttt aaattatgga ctgttttttc
     1681 aattacttct gcttttgccc ctaaaagata taacttgtac tccttctcta cagctaattg
     1741 gcaaatatgt ttaaccatat caatcccagt aatacgttca gaaaatggtt cttccagaaa
     1801 agcagtcgcc cataatattc cagctccatc tggggtaacc atatctgctt ttttgg
//
LOCUS       DAOQWT010000351         1850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_75087_length_1850_cov_2.201671, whole genome shotgun sequence.
ACCESSION   DAOQWT010000351 DAOQWT010000000
VERSION     DAOQWT010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75087_length_1850_cov_2.201671"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1820
                     /locus_tag="PKJ95_06745"
     CDS             <1..1820
                     /locus_tag="PKJ95_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HNR65969.1"
                     /translation="KIRDWGYLVNTIHGGMKLEERVEAEKIFKNETQILVATEAAGEG
                     INLQFCHMMLNYDIPWNPNRLEQRMGRIHRYGQQKEVFVFNLVTEDTREGMVLKKLFD
                     KLEEIKQSLGSDKVFDVLSDILYDKSLSQLLMEASANSRSIQEILSELDITVDEEYIS
                     KVKENLGESLATRYIDYTRIKEMSQQAREHRLIPEYTESFFKKAFIKAGGKIKEKQDG
                     FLSIDSIPYDIREIEKIDTFRKSYGRMLKKYPKVIFDKAIAFKNPDAEFISFGHPIFE
                     AVMVWIENSFSDSLLNGATFIDPDGNLDGIILFYIGEITDGNHEVAGKRLFSFYVKDK
                     TVQPVPPSIIWDLAESNKTESETIDLESIKSLVIKTSISELKIYKEYLSGERLRQAEI
                     KGKYGIKSLEYLVVKLDQELISLYSRKNAGENVDLAIRNKEDRKEDYDKARVKLEEQI
                     KREKTLNMSMPHFIGIIRVKPGKSDNGMHSDPEIEKIGMKVAMEHEIKNGRKPEDVSS
                     ENLGFDIRSTDKAGSVRYIEVKARAKVGSVALTQNEWFKAKRFKDDYYLYVVLNASNE
                     AKLYVIRNPAETIKPEEKVEVVRYIITLDNIKEKAVNIK"
BASE COUNT          668 a          243 c          400 g          539 t
ORIGIN      
        1 agaaaatcag agactggggt tatcttgtta atacaattca cggtggtatg aaactggaag
       61 aaagagtaga ggcagaaaag atatttaaga atgaaactca gattcttgta gcaacagaag
      121 cggcaggtga aggtattaat cttcaattct gtcatatgat gctaaactat gatattccct
      181 ggaatcccaa tagacttgaa cagaggatgg gccgtattca ccgttacggt cagcagaaag
      241 aggtttttgt ttttaatctt gtaactgagg atacaagaga aggaatggtt cttaagaaat
      301 tattcgataa actcgaagaa ataaagcaat ctctgggcag tgataaagtc tttgatgtat
      361 tgagtgatat tctctatgat aagagcctct cacagctttt aatggaagcg tctgccaatt
      421 cgaggagtat tcaggaaata ctcagtgaat tagatattac agtagatgaa gagtatatat
      481 caaaggttaa ggaaaacctt ggagaaagtc ttgcaaccag atatattgat tataccagaa
      541 taaaggaaat gtctcagcag gcacgggagc accgtcttat tcccgaatat acggaaagtt
      601 tctttaaaaa agcttttatt aaagcaggag gaaaaataaa ggaaaagcag gatggtttcc
      661 tctctataga ttctatccct tatgatataa gagaaattga gaaaattgat acttttagaa
      721 aatcctatgg ccgtatgtta aagaaatatc cgaaagttat ctttgataag gctattgcat
      781 ttaaaaaccc tgatgctgaa tttatttcct ttggtcatcc tatttttgaa gcggtaatgg
      841 tctggataga gaatagtttt tccgactctc ttttaaacgg ggcaactttt atagatcctg
      901 acggtaatct tgatgggatt atactttttt atataggtga gattactgat ggaaaccatg
      961 aagtggcagg gaaaaggctc ttttcttttt atgtaaagga taaaacagtt cagcctgtgc
     1021 ctccttccat tatctgggat ctggcagaaa gtaataaaac agaaagtgaa actattgatt
     1081 tagaatctat aaaaagtctt gtaattaaaa cttctatttc agaacttaag atatataaag
     1141 aatatctgtc aggagagcgt ttaagacagg cagagataaa gggaaaatat ggtattaaat
     1201 ctctggaata tcttgtagta aaactggatc aggaattaat ttccctttat tcccgaaaaa
     1261 atgccggtga aaatgtagac cttgccatca ggaataaaga ggacagaaaa gaggattatg
     1321 ataaggccag agttaaactg gaagaacaga taaaaagaga aaagactctt aatatgtcta
     1381 tgcctcattt tattggtatt ataagggtta aaccgggtaa atctgataat gggatgcaca
     1441 gtgatcctga aattgaaaag ataggcatga aagtggctat ggagcatgaa ataaaaaatg
     1501 gacgtaagcc ggaagatgtt tcatctgaaa accttggttt tgatatacgt tctaccgata
     1561 aagctggttc tgtaagatat attgaagtta aagcaagggc aaaggttgga tctgtggctt
     1621 taacacagaa tgaatggttt aaagcaaaga ggtttaaaga tgattattat ctttatgttg
     1681 ttttgaatgc aagtaatgag gctaagcttt atgttataag gaatcctgct gagactataa
     1741 agccggaaga gaaggttgaa gtagtaagat atattattac actggataat atcaaagaaa
     1801 aagcagttaa tataaaatag ataaatctag ttaactaaat caaaggaagg
//
LOCUS       DAOQWT010000352         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_75211_length_1848_cov_2.731177, whole genome shotgun sequence.
ACCESSION   DAOQWT010000352 DAOQWT010000000
VERSION     DAOQWT010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75211_length_1848_cov_2.731177"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..>1848)
                     /locus_tag="PKJ95_06750"
     CDS             complement(71..>1848)
                     /locus_tag="PKJ95_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNR65970.1"
                     /translation="PIISVLDSKGRLNENAGPFNGLHYKEGNKKIIEALREKGYLLGA
                     GKIRHSYPHCWRCKNPILFRATEQWFVSVDKFREQSLNAINGVQWIPGWGREKIYDMV
                     AERTDWCISRQRVWGVPIPAFYCQECKEIVITPETINIVKELFREKGSNSWFKMSAEE
                     ILPKGFQCPYCRGTVFEKEKDIMDVWFDSGCSHAAVLMNRKELHWPCELYLEGSDQHR
                     GWFQTSLLVAVAAFDNAPFKSVLTHGFIVDEKGKKMSKSIGNVIPPQEVIDQFGADIL
                     RLWVASSDYKKDIRISKKILQQLVEIYRKIRNTSRFILGNLYDFNPVQHTVPYEQLEE
                     IDKLILSQFQTLIEMTTANYEQYEFHSLYHDIHNFCALNLSSFYLDIIKDRLYVLASD
                     SLSRRAAQTVLYNLISGLTRLIAPVLSFTAEEIWQILNKFYEQEESVFLAEWPQVNRN
                     LLQIELEKKWDKLLAVRKDVLKALEIRRKDGLIGNALQAQVDLYTGNKSLYDYLNQNK
                     EKLEMLFIVSKVNLFSSKEQISEDNVFQGEEVPELSIVIKVAPGQKCERCWCYSETVG
                     SNNRYSNVCARCASVLESEGNNCEQ"
BASE COUNT          554 a          404 c          281 g          609 t
ORIGIN      
        1 ttcatttgta cccacactct ttatctttgt attttgacta aaaaccaaaa actatgaact
       61 aaaaactctt ctattgctca cagttattgc cttcactttc cagcactgag gcacatctgg
      121 cacaaacatt actataacga ttattacttc ccactgtttc agaataacac cagcatcttt
      181 cacatttttg accaggggca acttttataa ctatagaaag ctccgggact tcttcccctt
      241 gaaagacatt atcttctgat atctgctctt tgcttgagaa tagattaact tttgagacga
      301 taaagagcat ttccagtttt tctttattct gattcagata atcatacaag gatttattcc
      361 cggtataaag atcaacctga gcttgaagag cattaccaat aaggccatcc ttcctgcgta
      421 tttccagagc cttcaaaaca tcctttctaa ccgctaacag tttatcccat ttcttttcca
      481 gctctatttg taataaattt ctatttacct ggggccattc ggccaggaag acactctcct
      541 cctgttcata gaacttgttt aaaatctgcc atatctcttc agctgtaaaa cttagtaccg
      601 gggctatcaa tcgagttaat ccacttatta agttatataa gacagtctga gctgctctac
      661 gggataaaga atctgaagct aaaacatata gcctgtcttt aattatatca agatagaacg
      721 aacttaaatt taaggcacaa aaattatgaa tatcatgata aagagaatga aattcatatt
      781 gttcatagtt tgcagtagtc atttctatta aagtctgaaa ctggctcaaa ataagtttat
      841 caatttcttc taattgttca taaggcactg tatgttgaac aggattaaaa tcatacagat
      901 ttcccaggat aaagcgagag gtattcctta tttttcggta tatctccact aattgttgta
      961 atattttttt agaaatcctg atatctttct tgtaatccga ggatgctacc cagagtcgca
     1021 ggatatctgc tccaaactga tctattactt cctgcggtgg aattacatta cctatcgatt
     1081 ttgacatttt tttacctttt tcgtcaacta taaaaccatg tgtcaatact gacttaaatg
     1141 gagcattatc aaaggctgct accgccacca aaagtgaggt ctgaaaccaa ccacggtgtt
     1201 ggtctgaacc ttccaggtat aattcgcaag gccagtgtaa ttcttttctg ttcatcaata
     1261 cagcagcatg gctgcatcct gaatcaaacc agacatccat aatatctttt tctttttcaa
     1321 atactgtgcc ccgacaataa ggacattgaa atcctttggg aagaatctct tcagcactca
     1381 ttttaaacca ggaattagaa cccttttctc taaacaattc cttcactata ttgatggttt
     1441 caggagtaat aactatctct ttgcattcct gacaatagaa agcagggata ggaactcccc
     1501 atactctctg gcgtgaaatg caccaatcag tcctctctgc aaccatatca taaatcttct
     1561 ctcttcccca gccgggaatc cattgaaccc cattaattgc attcaaagat tgctctctga
     1621 atttatcaac agaaacaaac cattgttcag tagctctgaa caggattgga tttttacagc
     1681 gccagcaatg agggtaagag tgccttattt tacctgctcc taagagatat cccttttctc
     1741 ttaaggcctc aattattttt ttattccctt ctttatagtg aagtccatta aaaggtccgg
     1801 cattttcatt gagtctgcct ttactatcca gtacagaaat aatgggca
//
LOCUS       DAOQWT010000353         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_75608_length_1842_cov_3.284835, whole genome shotgun sequence.
ACCESSION   DAOQWT010000353 DAOQWT010000000
VERSION     DAOQWT010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75608_length_1842_cov_3.284835"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1101
                     /locus_tag="PKJ95_06755"
     CDS             193..1101
                     /locus_tag="PKJ95_06755"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNR65971.1"
                     /translation="MRAQKIWMDGKFVDWDEAKVHVLTHALHYGSGVFEGIRAYKTDQ
                     GTAVFRLREHVERLFNSAKILRLEMSFSKEDISHAIKETIRINNLESCYIRPLVYRGY
                     NKLGLNPFTCPVQVMIAAWEWGAYLGEEALAKGVKAIISSWQRSHINSTSAKAKLCAN
                     YINSIFANMEATELGAEEAILLDSNGFIAEGPGENIFWVKGGIIYTPPLSAVLEGITR
                     SSLITMARDIGYEVKEEFAARDVLYIADEAFFVGTAAELTPIREVDGYIIGSGERGPI
                     SKVIQEKLFNIFKGKEKEYLDWLEFV"
     gene            complement(1284..>1842)
                     /locus_tag="PKJ95_06760"
     CDS             complement(1284..>1842)
                     /locus_tag="PKJ95_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNR65972.1"
                     /translation="FQIKNTTEQILIQYLIEKEIVEKTEINDDEIKSYYDEHKENYFR
                     EEQIHVLNILTETEEEAIDALKKLEEGIDFSILAQEVSIAASVSKGGDIGFISKGTLN
                     ADIEDQLFILDSGDLSEIITTDKGFNIFKIIEKVSSYYLELDEVREEIKYQLLPQKQQ
                     QAFDQYLKDIEERATIEKNLELIQE"
BASE COUNT          610 a          267 c          327 g          638 t
ORIGIN      
        1 taattgagga acttactgaa tccaaagttt ctattgtttc cgttgggcca aagagaacac
       61 agaccatttt cagataattg tttttttgaa ttataattta ccaatatttg tagatatagt
      121 atattatata aatgataatg ataatgtaat tcccctaatc ttaaaataaa aatattggga
      181 ggtatttaaa agatgagggc acaaaaaata tggatggatg gaaaatttgt cgattgggat
      241 gaggcaaagg tgcatgtact gacacatgct ttacactatg gaagcggtgt ttttgaaggg
      301 attagagctt ataagactga ccagggaact gccgttttca gactaagaga acatgtggaa
      361 cgcttgttta attctgcaaa aatattgaga ttagaaatgt ctttttcaaa agaagatata
      421 tcccatgcaa taaaagaaac gataagaatt aacaatttgg aaagttgtta tatcagacca
      481 ctggtataca gaggatataa taaacttggc ctcaatcctt tcacctgtcc ggttcaggtt
      541 atgatcgctg cctgggagtg gggggcttac ttaggtgagg aggccttggc aaaaggagtt
      601 aaagcaataa tatcatcctg gcagagaagc catattaatt caacttctgc aaaagctaaa
      661 ttgtgtgcta attacattaa ttcaattttt gccaatatgg aggcaacaga attaggagca
      721 gaggaagcaa tccttttaga tagtaatggg tttatagctg aaggccctgg agagaatatc
      781 ttttgggtaa aagggggaat tatctataca cctcctttat ctgctgtatt agaaggaatt
      841 accaggagtt cacttatcac catggcccgg gatataggct atgaagtgaa ggaagaattt
      901 gcagcgcgtg atgttctcta tattgcagat gaagctttct ttgtaggaac tgcggccgaa
      961 ttaaccccta ttcgtgaagt agatggctat attatcggtt ctggagaaag aggcccaata
     1021 agtaaagtta ttcaagaaaa actatttaac atatttaaag gcaaagaaaa agaatattta
     1081 gattggcttg agtttgtata ataaatagtc atctaaaata tctgaaatag aaattattca
     1141 tttagtgcag aataaaaggg gaaatggcct tagtgattaa attatcatta aggccataaa
     1201 ttattataaa ggatattagt actttctata gcatattttc atttcaattt ttatttattg
     1261 tgtttattaa tattaacttt aatctattct tgaattaatt ctaaattctt ttctatggta
     1321 gctctctctt caatatcttt taaatactgg tcaaaagctt gctgttgttt ttgtggtaat
     1381 agttggtatt ttatctcttc tcttacttca tctaattcta aataataaga ggagaccttt
     1441 tcaattattt taaaaatatt aaacccttta tcggtagtta taatttcact gagatcgcct
     1501 gaatccaaga taaataattg gtcctcaata tcagcattta aagtgccttt actgataaaa
     1561 cctatatctc cccctttaga tacactggca gcaatagata cttcttgtgc caaaattgaa
     1621 aaatctatac cctcttctaa ttttttaagg gcatcaattg cttcctcttc agtttcagta
     1681 agaatgttta aaacatgaat ctgttcttct ctgaaataat tttctttatg ttcatcataa
     1741 taagacttaa tttcatcatc atttatttca gttttttcca cgatctcttt ttcgattaaa
     1801 tattgaatta aaatttgttc agtagtattt ttaatttgaa at
//
LOCUS       DAOQWT010000354         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_75706_length_1841_cov_2.598544, whole genome shotgun sequence.
ACCESSION   DAOQWT010000354 DAOQWT010000000
VERSION     DAOQWT010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75706_length_1841_cov_2.598544"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..873)
                     /locus_tag="PKJ95_06765"
     CDS             complement(112..873)
                     /locus_tag="PKJ95_06765"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNR65973.1"
                     /translation="MKNFNNLQKHPALVGIVPRKNLWDIIQKQRWYHIPVRSAPENAG
                     FAEYLGFYFPKIFGETMRYKVNFYAQVKKVDIVKRIQLFPEEKEHLRANQDYYHFHLG
                     KIKELPKPIPSLRWRRIVHIPTSCEKLFTAEEVNDLYDTSPLEEKMYLEMKKREIPAE
                     RQFYVKVEGGFYCLDFGIFCKKGNIDVECDGEKYHILPEALTKDRERNNELTSFGWSV
                     LRFTSKQINQTINNCFTKIERTIGNLGGILGDYLL"
     gene            complement(1326..>1841)
                     /locus_tag="PKJ95_06770"
     CDS             complement(1326..>1841)
                     /locus_tag="PKJ95_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNR65974.1"
                     /translation="EIARKEDGFYVDQFNNLSGVAAHFNSTGPEIWQDMPDIDAFVAA
                     VGTGGLFVGTSKFLKSKNNKIKCIAVEPAESAILKTGKVNNPQHIIQGTGYGLVPPHW
                     VDSLADDIVSVTDNEVLEMTRRLSSEQGLYVGYSSGANVFAALKNATLHPGIKNIVTI
                     LCDTGYKYSDL"
BASE COUNT          583 a          385 c          206 g          667 t
ORIGIN      
        1 aaagacccgc ccccccccag ttttatcttt tgtagcaaat tttccaaatc agattcaata
       61 caaaaatcct aaaacttctt ttgttgatat gaatcatttt accatcttta tctacaataa
      121 ataatctcct aaaattccac ccaaatttcc aatagttctt tcaattttcg taaaacaatt
      181 atttatggtt tgattaattt gtttacttgt aaatcttaaa acactccagc caaaacttgt
      241 taattcgtta ttcctttctc tatctttcgt taacgcttca ggtaaaatat gatatttttc
      301 accatcacat tccacatcta tattgccttt tttacagaat attccaaaat ccaaacaata
      361 aaatcctccc tcaactttta cataaaattg tctttctgca ggtatttctc ttttcttcat
      421 ctctaaatac attttctctt ctaaaggact ggtgtcgtac aaatcattga cctcttctgc
      481 ggtgaataat ttttcacaac ttgtaggaat atggactatt ctcctccatc ttaaacttgg
      541 aataggtttt ggtaattctt taatttttcc taaatgaaaa tgataataat cttgattcgc
      601 tcttaggtgt tctttttctt ctggaaaaag ctgaatcctc tttacaatat ctactttttt
      661 aacttgggca taaaaattta ctttataacg cattgtttca ccaaaaatct tgggaaaata
      721 aaaacccaaa tactcagcaa atccagcatt ttccggtgct gatctaactg gaatatgata
      781 ccatctctgt ttttgaatta tatcccaaag atttttccgc ggaacaatcc ccaccaaagc
      841 cggatgcttt tgcaaattat taaaattctt cataattatt aaccttttaa tttatcctta
      901 aaattctatg ttagtttgaa tgaaaccctt attttcgctt ttttgtaatt attttgttgt
      961 aaaacaattt accctcttct ttcgaaaatt aataagtaat atatttaaat aaatctattg
     1021 ctatgagtta aaaaaataac ttattttcca gaatttcttc aaattgttta ttttccactc
     1081 ttttaaaaaa atagaaatct tctccattaa actaaaaaat aactatacat ctcaaataaa
     1141 aaatcagggt catctaccga atatccggat ttagagccaa tacagaattt ataaaacaaa
     1201 aaggacacat gactgttaac cttttagatt atcttcatca caccaattaa atatttattg
     1261 ctgtttttaa atattcaata catccttatt ttcttctttt ctaaaactat catcccgata
     1321 cattcttaca aatcggaata tttatacccg gtatcacata aaatagtcac tatattttta
     1381 attcctggat gaagtgttgc attttttaag gcagcgaaca cattggcacc agatgaatag
     1441 ccaacataga gcccctgctc agaactcaac cttctggtca tctccagcac ctcattatca
     1501 gtaacagaca ctatatcatc tgccaatgaa tcaacccaat gtggtggcac cagcccatat
     1561 cctgttccct gtataatatg ctgtggattg tttactttac ccgtcttcaa tattgcagat
     1621 tctgccggct ctactgctat gcattttatc ttattattct ttgattttaa gaatttagat
     1681 gtccctacaa atagtccgcc agtaccaacc gccgctacaa aagcatcgat atctggcatg
     1741 tcctgccaga tttctggccc tgttgaatta aaatgtgccg caacaccaga aagattatta
     1801 aattggtcaa cataaaaccc atcctctttt cgggctatct c
//
LOCUS       DAOQWT010000355         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_75939_length_1837_cov_3.555556, whole genome shotgun sequence.
ACCESSION   DAOQWT010000355 DAOQWT010000000
VERSION     DAOQWT010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75939_length_1837_cov_3.555556"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKJ95_06775"
     CDS             <1..238
                     /locus_tag="PKJ95_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HNR65975.1"
                     /translation="LLIATKGTHITPENPQETLRSSVIFDPEDFKIEHSRKPDTYYEL
                     IETEYPGRRYLELFARAQRPGWETFSNEPELRAA"
     gene            309..605
                     /locus_tag="PKJ95_06780"
     CDS             309..605
                     /locus_tag="PKJ95_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65976.1"
                     /translation="MDTIKSIRTEDSHTGTVLIKFEKNPFSVRILIRPTPRAYGLLQL
                     TDQDGKTSIIQYRGADLKNFSIFRKFLQSQGFTFTGNTTDFKLFKLFLFSSVKE"
     gene            611..745
                     /locus_tag="PKJ95_06785"
     CDS             611..745
                     /locus_tag="PKJ95_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65977.1"
                     /translation="MLQEPPTEKQMAYLKALKYRGKPPASKAEASDLIATIKTFQKLQ
                     "
     gene            759..>1837
                     /locus_tag="PKJ95_06790"
     CDS             759..>1837
                     /locus_tag="PKJ95_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65978.1"
                     /translation="MDFVVSTNKAAELIDYFKRMGVKKDHIFHDGNGTIALAWQCFDW
                     DPVSEKVFTAIAARIAKETKSMISFITDKPVELVKPDKRYFHLKGMVVAEKLQIEDPK
                     EISEKKTEPPPVQPPEREKTFYEIPENYIFTEFCYTWENKQKGTSGFTKWEPCTLRKS
                     KKYPHLPTVAEVKHKYKRKELFFSQFRFADTTENFNREKAHRYFKYGEFIMESDNPAA
                     VEFSRKVISFLEKTLHIPPEAISIDCSGGRSIHIGIAPEIFGVTPCANLPEQYEELAA
                     MITEETGTIFDLKIYKPRRMFRAYGAYRPSKGTYKIAITREELFNLSMDQILELARTE
                     RPGTRRTAAGIVPEAKAIFEKAKKQA"
BASE COUNT          651 a          388 c          387 g          411 t
ORIGIN      
        1 acttctgata gctacaaaag gaactcatat aacgccggaa aacccacagg agacccttag
       61 aagttctgtt atttttgacc cggaagactt taagattgaa cactcccgga agcctgacac
      121 atattatgaa ttgatagaaa ccgaataccc aggacgccga taccttgaac tattcgcccg
      181 cgctcaacgc ccaggatggg aaacattctc aaatgagcca gagttaagag ctgcatagat
      241 agcattaccc ctgccgggaa aatcgcaaat agaagatccc ggcgcagggg aaacaaagga
      301 gtaaaacaat ggacacaata aaaagtataa gaactgaaga tagccacacc ggcaccgtat
      361 taataaaatt cgaaaagaat cctttttccg tgagaatact tatcaggccc acgccccgcg
      421 cctatggatt attgcaatta accgaccagg acgggaagac ctctattatt caatacagag
      481 gcgcagatct aaagaacttt tccatattca ggaaattcct acagtcccag gggttcactt
      541 ttaccgggaa cactactgac tttaaacttt tcaaattatt tttattttct tcagtaaagg
      601 agtgatggcc atgctgcagg aacccccgac agagaaacag atggcgtatc taaaagcgct
      661 taaatataga ggtaaaccgc cggcaagtaa agcagaagca tcagacctaa tagctactat
      721 aaaaactttt cagaagctac aatagaagga gagagagcat ggactttgtt gttagtacga
      781 ataaggcagc agaactgata gattacttta aacgtatggg agtaaagaaa gatcatattt
      841 ttcacgatgg aaacggaacc atcgcacttg catggcaatg ttttgactgg gatcctgtat
      901 cagagaaagt attcactgct atagctgcca gaatagcaaa agaaacaaag tccatgatct
      961 cttttattac tgataaaccg gttgaactgg taaaacctga caaaagatat ttccatttaa
     1021 agggtatggt tgttgctgaa aaattgcaaa tagaagatcc gaaggaaatt tcagagaaaa
     1081 agacagaacc gccaccggtg cagccaccgg aacgagaaaa aaccttctac gaaatacctg
     1141 agaactatat ttttactgag ttctgttata catgggagaa taaacagaag gggacatctg
     1201 gatttacgaa atgggagccc tgcaccttac ggaaaagtaa aaaataccca cacctgccca
     1261 cagtcgcaga agtaaagcat aaatacaaaa gaaaagagtt attcttctct caattcagat
     1321 ttgccgatac cacagagaac tttaacaggg aaaaagcgca caggtatttt aaatatggtg
     1381 aatttattat ggagagtgat aaccctgctg cagtagaatt cagcaggaaa gttatttcct
     1441 tcttagagaa aaccctgcac atcccgccgg aagcaatatc tattgactgt tcaggaggaa
     1501 gaagcatcca tataggaata gcacctgaaa tattcggagt aacgccatgt gcaaacctcc
     1561 cggaacaata cgaggaactt gcagcaatga ttaccgaaga aaccggcacc atatttgact
     1621 tgaagatcta caaaccccgt cgtatgttcc gcgcttatgg cgcttataga ccgagtaagg
     1681 gcacctataa gatagccatt accagagaag aacttttcaa cctttctatg gatcagatat
     1741 tagaacttgc caggacagag cgcccaggga cacgaaggac agcagccgga atagtcccag
     1801 aagcaaaggc aatcttcgaa aaagcaaaga aacaggc
//
LOCUS       DAOQWT010000356         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_76004_length_1836_cov_2.821449, whole genome shotgun sequence.
ACCESSION   DAOQWT010000356 DAOQWT010000000
VERSION     DAOQWT010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76004_length_1836_cov_2.821449"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..700)
                     /locus_tag="PKJ95_06795"
     CDS             complement(107..700)
                     /locus_tag="PKJ95_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR65979.1"
                     /translation="MYNQKIRKLTIVLFLLTVFLLGGCNQLPGGSCVNEVQFVDKNLE
                     QVVRVKINKLSGPLYQSDVVNIFELDAEKKGIVSLSGIEHLKNLRELDLDDNQISDLS
                     PLAGLTKLKELDLENNNISDLSSLTNLINLEELDFENNKVTDISALVSNKGISMGDEV
                     DMRYNHLDLSEGSKNMQDINTLINRGVKVEYIPQSNH"
     gene            complement(788..1006)
                     /locus_tag="PKJ95_06800"
     CDS             complement(788..1006)
                     /locus_tag="PKJ95_06800"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNR65980.1"
                     /translation="MELSGLINKKSQVFKKLAVTLTEFDDRGIIQLIEGKPRIVIRPI
                     LTEGKHILFGFKEQNYLDFIEAGENPLA"
     gene            1443..>1836
                     /locus_tag="PKJ95_06805"
     CDS             1443..>1836
                     /locus_tag="PKJ95_06805"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1578..1580,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNR65981.1"
                     /translation="MLVTKHAPNFTGSAFYQGKYKKVSLEDYRGKWVVLCFYPGDFTY
                     VUPTEISHIAVGYDQLQALGVEVLSVSTDSVHSHKIWNETELSKMIGNDVPFPMVSDQ
                     NGAIGKLYDVYDEASGINVRGRFLIDPEG"
BASE COUNT          559 a          331 c          282 g          664 t
ORIGIN      
        1 tacaataata aaaaagagtg tgattgaaaa gtttctaaat cgtactcttg aattatctga
       61 ttattacttt tattaataac tattggtaat ttggcaataa ttattattag tgattacttt
      121 gaggaatata ctcaaccttc acacctctat taattaaagt attgatatcc tgcatatttt
      181 tagagccttc gctaagatcg agatggttat atctcatatc tacttcatca cccatactaa
      241 tccctttatt actcaccagg gctgagatat ctgttacttt gttgttctca aaatctaact
      301 cctccagatt aattaagttt gtcagggaag acaggtcgga aatattgttg ttttctaaat
      361 ccagctcttt tagtttggtt aagccagcta aaggagaaag atctgatatc tgattgtcat
      421 caaggtccag ttcacgtaaa tttttaaggt gttcgattcc ggacaatgaa acgattcctt
      481 ttttctcagc atccaattca aagatattta ccacatcact ctgatacagt gggccactta
      541 gtttattaat tttcacccta actacctgct ctaaattttt atctacaaat tgtacttcat
      601 tcacacatga gccaccaggc aactgattgc atcctcccag taagaatact gttaataaaa
      661 ataatactat tgttaatttt cttattttct ggttatacat tattttgaac ctccgtttaa
      721 actaattttt ttacaagaat tcttcataat catttttggt aagttgtaat aaatgacggg
      781 ttgttagtta tgctaatgga ttttctcctg cctcaatgaa atccagataa ttctgttctt
      841 taaatccaaa aagtatgtgc tttccttctg ttaagatcgg tctgataacg atacgaggct
      901 taccctctat caattgaata atacccctat catcaaactc ggttaaagtt actgccagct
      961 tcttaaaaac ctggctcttt ttattaatta aaccagataa ttccaatccg gcaaatttag
     1021 ccagcccttt aatctctttt tcggatagag gattcttgat aagattccga tattcgaaat
     1081 caatattatg ctgcgaaagc cacgctttcg ccttgcggca ggtgcttcag gtatctatac
     1141 aataaaatat cattttttga ctcattattc ttaacctccc attatgacaa ttttgtgaaa
     1201 tgcttttatt tcttacattt atttatctct ttgtttgact atttaatcaa ccctgatagg
     1261 ttatctctct ttgataattg gcttattcct atggttttta ttataacatt aataccattt
     1321 taaatctaag gtagttatct tatgccttta aaactgctgc tcttcattat tgacttttat
     1381 gcttatcaat gctaaattgt aattaatact atttaatatt taatacgaaa ggagtagtat
     1441 aaatgttagt tactaaacat gcaccaaatt ttacaggttc agcattctat cagggaaagt
     1501 acaaaaaagt atctttagaa gattaccgtg gtaaatgggt tgttttgtgc ttctatccag
     1561 gtgatttcac ctacgtctga ccaaccgaaa tatcacacat agccgtgggc tatgaccaac
     1621 ttcaggcatt aggtgtagag gtgctttcag taagcacaga cagtgtacat tcccacaaaa
     1681 tctggaatga aactgaatta agtaagatga ttggcaatga tgtgcccttc ccaatggtat
     1741 cagatcaaaa tggtgctatt ggaaaacttt atgatgtcta tgatgaggca tcaggaataa
     1801 atgttcgggg aagattttta attgacccgg aaggta
//
LOCUS       DAOQWT010000357         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_76820_length_1824_cov_2.767100, whole genome shotgun sequence.
ACCESSION   DAOQWT010000357 DAOQWT010000000
VERSION     DAOQWT010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76820_length_1824_cov_2.767100"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PKJ95_06810"
     CDS             <1..1203
                     /locus_tag="PKJ95_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65982.1"
                     /translation="ISENYLYTVEGMVDFWEHLSSDGIICITRWLLFPPRESVKLTAI
                     ALEALEKIGVENPENHIALIRSWGTVTFICSKRAIDRKKADTIISFCGERSFDPIYFP
                     GILPEQVNQNHRLEKPYYYQAISELIENYKNDEAKTFFQKYFYNVTPATDNSPFFFSF
                     LKWRTIPVIIRDTSYWQPVLEWGNLMVFATLAQGIIFSFFFIFLPLLVRKVSPGEGWY
                     FLLLYFAALGFGYMLVEVSFIQKFILYLTHPTYATSAIIFSFLFFSGLGSRFSQRWQA
                     RESDTLYYLKRIILFIGLCLILYQILIPAIFKYTLHLSLVVRFLLTSFLIAPLAFLMG
                     MPFPLGIYFLAREREGMIPWVWATNNFCSILASILAVMIAITSGFKVVGYLAAGIYLV
                     GLLAISKK"
     gene            complement(1377..>1824)
                     /locus_tag="PKJ95_06815"
     CDS             complement(1377..>1824)
                     /locus_tag="PKJ95_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNR65983.1"
                     /translation="YELRERGLILKLFDGYRPQVAVNHFIRWAEDVEDQKMKDKYYPQ
                     VNKRDVFKLGYIAKRSGHSRGSTIDLTLVDITTGEELDMGSGFDFFGEISHHGTDKIG
                     EEQFQNREILKNAMLKHGFELYSKEWWHYTLSDEPFPSTYFDFPVQ"
BASE COUNT          460 a          359 c          388 g          617 t
ORIGIN      
        1 atctcagaaa attatcttta taccgtggag gggatggttg atttttggga acatctctcc
       61 tctgatggta taatctgcat tacccgctgg cttcttttcc ctccccggga atcggtcaaa
      121 ctgacagcta ttgctttaga agcgctggaa aagatagggg ttgagaatcc cgagaatcat
      181 attgccctga ttcgcagctg gggtacggtt acttttatct gtagtaaaag agctattgat
      241 aggaagaagg cggacactat tatttctttc tgtggggaga ggagttttga cccaatttac
      301 tttcctggaa ttcttcccga acaggtcaat cagaaccatc gtctggaaaa gccttattat
      361 tatcaggcta tttcagaact aatagaaaat tataagaatg atgaggcaaa gacatttttc
      421 cagaaatatt tttataatgt tacacctgct actgataata gtcctttctt cttctctttt
      481 ttaaagtgga gaaccatccc cgttattatc agggatacca gttactggca acctgtgctg
      541 gaatggggta atctgatggt ctttgccacc ctggcacagg gaataatatt cagcttcttc
      601 tttatattcc tgccacttct ggttaggaag gtatcgccgg gtgaggggtg gtattttctg
      661 ctgctttact ttgccgcgct gggctttggt tatatgctgg ttgaggtctc ctttattcag
      721 aaattcattc tttatcttac ccatcccact tatgccacct cagcaatcat cttctctttt
      781 ttgttctttt cgggactggg gagccgtttc tctcaacgct ggcaggcgag agagagtgat
      841 acattatatt atctgaaaag gattattcta ttcatcggtc tatgtttgat actgtatcag
      901 attctgatac cggctatttt taaatatacc cttcatctat ctttagtggt gagattttta
      961 ctgacttcat ttctgatagc accactggca ttcctgatgg gtatgccttt cccccttggt
     1021 atctattttt tggccaggga aagggagggg atgataccct gggtatgggc tactaacaat
     1081 ttctgctcta ttctggcatc tatcctggca gtgatgattg ctattaccag tgggtttaag
     1141 gttgtggggt acctggcagc aggcatttat ttggtgggat tattggcaat ttcaaagaaa
     1201 taaattagta gtatgccgat aaagcaggta tggctggtat ctcttaagat gttatgcaca
     1261 gtagcacaga gtagttttgt ttggaatatc attttttatg aatttttaag atcactacct
     1321 gacattagtg aattttaatt cttttaaaag atgttaaagg accgtcccct aacatgttat
     1381 tggactggga aatcgaaata ggttgaaggg aatggctcat ctgaaagggt gtagtgccac
     1441 cattctttgg aatacaattc aaacccgtgt tttaacatag cattttttaa aatctctcta
     1501 ttctgaaact gttcttcacc gattttatcg gtaccgtggt gggagatttc tccaaaaaag
     1561 tcaaagccgc tgcccatatc cagctcctca ccggtagtaa tatcgacaag agtgaggtct
     1621 attgtacttc ctctcgaatg gcctgacctt ttagctatat agccaagctt aaagacgtct
     1681 cttttgttta cctgtggata atatttatct ttcattttct ggtcttcgac atcctcagcc
     1741 cagcggataa agtgattgac agccacctgt gggcgatagc catcaaataa ttttaaaatc
     1801 agcccgcgtt ctcttaattc atag
//
LOCUS       DAOQWT010000358         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_76821_length_1824_cov_2.761447, whole genome shotgun sequence.
ACCESSION   DAOQWT010000358 DAOQWT010000000
VERSION     DAOQWT010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76821_length_1824_cov_2.761447"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="PKJ95_06820"
     CDS             complement(<1..1477)
                     /locus_tag="PKJ95_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65984.1"
                     /translation="MINIISQRKEVEFIQTFTQAYNSIKSTRKAIVFYYLDEKIIISD
                     IDFSPVAELLASLYSPDPRGRKLRDPVCVLRSLILMVMLNETSITRWAKRLRSDEFLS
                     VIAGWEPGKSPSVGTFYDFLHRLQDGPYQKKCSHIEKPSELDKGWHKRNFKKEDKKKK
                     KEQKKEVDHQKQDSVTQKLAEELLANSEQPRQNDLTKRLQDLLMELGVKPSIKKGLID
                     PMELELCGDGSSIVSGAKKQGKYICNCLKENNIRKCDCPRLYKDPDADFGYDPYRDEY
                     YYGDTMYQICYSSKGHDLPVNIIAGPASETDYTLSLKAFDSCIKTFREHEIPVNITAA
                     IMDAGHDAMGNYFYFRQKDIPALIPLNNRGAKEAYRDNIKLSSRGIPLCKGCIEMRHI
                     SYNKKKMKHSFGCPVKRGTHRKGTYIYISHTEECPLGALCSPDTKHGPVVSISSRDNS
                     RLFPDIPRSSQKFKELYKLRTGTERSNAAKKEAYPFYRARLK"
BASE COUNT          532 a          333 c          368 g          591 t
ORIGIN      
        1 atttaagtct ggctctataa aagggatatg cttctttttt agctgcatta gatctttctg
       61 tacctgttct gagcttatag agttctttaa acttctgtga agaccgtgga atatcaggga
      121 aaagtcttga attatctctg gaagaaatcg acacaacagg gccatgttta gtgtcaggag
      181 aacagagagc accgagagga cattcttccg tatgggaaat atagatgtaa gtgccttttc
      241 tatgagtgcc tcttttaaca ggacagccaa aggaatgctt catcttcttc ttattataac
      301 tgatatgcct catttcaata cagcctttac agagaggaat gcctctggag cttaatttaa
      361 tattatccct gtaagcttct ttagctcctc tgttgttaag tggaataaga gcaggaatat
      421 ccttctgtct gaaatagaag taattcccca tagcatcatg tcctgcatcc ataatagcag
      481 cagtaatatt gactgggatt tcatgttctc tgaaggtttt tatacaggag tcaaaagctt
      541 taagtgacag agtatagtca gtttcagaag cagggcctgc aatgatatta acggggagat
      601 catgtccttt agaggagtaa catatctgat acatagtatc tccgtaataa tattcatctc
      661 tgtatgggtc atatccaaaa tctgcatcag ggtctttata taatctggga cagtcacatt
      721 tcctgatgtt attttctttc aaacagttac agatatactt cccctgtttt tttgcaccgc
      781 tcactattga agaaccgtca ccgcagagtt caagttccat cgggtcaata agccctttct
      841 ttatggaagg ttttacaccg agctccataa gcaagtcctg aagtctttta gtaaggtcat
      901 tctgtctggg ctgttctgaa tttgccagta gttcttctgc aagtttctga gtaacagagt
      961 cctgtttctg gtggtcaact tctttcttct gttccttttt cttcttctta tcttctttct
     1021 tgaaattcct tttatgccag cctttatcca gttcagaagg tttctctatg tgagagcatt
     1081 tcttctgata agggccgtcc tggagtctgt ggaggaaatc ataaaaagta cctacagaag
     1141 gagatttacc gggctcccaa cccgctataa cagacaggaa ttcatccgaa cgcagcctct
     1201 ttgcccatct ggtaatactt gtttcgttta acattaccat aaggataagg gaacggagaa
     1261 cacatacagg atcacgtagt tttcttcctc tcggatctgg agaatagagg gaagcgagta
     1321 attctgctac gggagagaag tctatgtcgg aaattataat tttttcatct aaatagtaaa
     1381 aaactattgc ttttctggtg gattttatac tattgtaagc ctgcgtaaat gtttggataa
     1441 attccacctc ctttctttgt gatattatat ttatcacaga aaaggagtga tttttttaga
     1501 ggttttttaa aaaatttctt gtaattttat tttcgagagc ttaaaattga ggtagaattc
     1561 tggattaaga taatgaattt ttagaagaaa aaagtaattt ttccagagag aagaattttc
     1621 cgagaggact cactctgaaa gagattgaaa cttcaattaa aacgagggac gcctcgacgc
     1681 ctgggttcgc agaaacacag gagaaactct gaaagagatt gaaacgagat gttagagacc
     1741 gtgtcgaatt ttgtgaaatc aagcagaaac acaggagaaa ctctgaaaga gattgaaaca
     1801 gaattctctg ttaaattccc acag
//
LOCUS       DAOQWT010000359         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_76981_length_1822_cov_2.747595, whole genome shotgun sequence.
ACCESSION   DAOQWT010000359 DAOQWT010000000
VERSION     DAOQWT010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76981_length_1822_cov_2.747595"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKJ95_06825"
     CDS             <1..294
                     /locus_tag="PKJ95_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNR65985.1"
                     /translation="VLQGAISPLDGVEPDHLTIEQLVSRIKNEKIKEVILATNPNIEG
                     EVTASYIARLLKPLNIKITRIAYGIPIGGDLEYADEVTLTQALLGRQIFKDDK"
     gene            328..900
                     /locus_tag="PKJ95_06830"
     CDS             328..900
                     /locus_tag="PKJ95_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNR65986.1"
                     /translation="MKKITEVRWHGRGGQGAVTASKLLATSAMAEDKYIQAFPEFGPE
                     RMGAPIQAFTRISDTPIKIHSHVTNPDMVVVLDATLIGPVDITEGLPDEGLIVVNTSE
                     TPAQIREKLNLKSRKIFTIDASKIAQEELGRPLPNTPMIGALIKATGLLDLKNVINDI
                     KNQFAGKFSQKVIDGNVKAIERAYQEVKEE"
     gene            897..1208
                     /locus_tag="PKJ95_06835"
     CDS             897..1208
                     /locus_tag="PKJ95_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNR65987.1"
                     /translation="MSAVKKTGWKEIPKGGLILEAGNSEQYETGSWRSQRPVWDEQKC
                     THCMICWVYCPDSSIIVEEGRVKGIDYKHCKGCGICAAECPTKAIAMKDENEFREKEG
                     V"
     gene            1211..>1822
                     /gene="porA"
                     /locus_tag="PKJ95_06840"
     CDS             1211..>1822
                     /gene="porA"
                     /locus_tag="PKJ95_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HNR65988.1"
                     /translation="MSIKTAAVTGNEAASYALRQINPDVVAAYPITPQTELVQSFSEY
                     VADGLVDTELITVESEHSAMSACVGAAAAGGRVMTATSANGLALMFEILFIAASNKLP
                     IVMPVVNRALSGPINIHCDHGDTMAARDAGWIQIYSENAQEVYDNLIQAIRIAENMKV
                     RLPVMVMFDGFIVSHGIERVDYLDDATVRNFIGTYQPNHPLLDV"
BASE COUNT          594 a          305 c          429 g          494 t
ORIGIN      
        1 gttttacagg gagctatttc acccctggat ggggtggaac cggaccatct gaccatagaa
       61 cagcttgttt cacgcataaa gaatgagaaa ataaaggaag tcattttagc tacaaatccc
      121 aatattgagg gagaagtaac tgcttcctac attgccagat tgcttaaacc tctaaatata
      181 aagataacca gaattgctta tggtatacct atagggggtg atttagaata tgctgatgaa
      241 gtaactctaa ctcaagcctt attagggaga caaatattta aagatgataa ataaggtaat
      301 aaattatgga gggaggcatt tcttattatg aaaaaaatta ctgaagtcag atggcatgga
      361 agaggtgggc agggtgccgt taccgcttca aagttactgg ccacttctgc tatggctgag
      421 gataaataca tccaggcttt tcccgaattt ggaccggaaa gaatgggtgc gccaatccag
      481 gcttttacca gaatcagtga cactccaatt aaaattcaca gtcatgtaac taatcccgat
      541 atggtggtgg tacttgatgc aacattaatt ggtccggttg atattactga ggggttgccg
      601 gatgagggtt tgatagtggt aaataccagt gaaaccccag ctcagattag agaaaaactt
      661 aatttaaaga gcagaaaaat ttttactatt gatgccagca aaattgccca ggaggagtta
      721 ggacgccctt tgccaaatac tcctatgatt ggagctttaa taaaagccac agggttatta
      781 gacttaaaga atgtgataaa cgacattaaa aatcaatttg ccggtaaatt cagccaaaaa
      841 gttattgatg gaaatgtgaa agcgatagaa agagcatatc aggaggtgaa agaagaatga
      901 gtgccgtcaa aaaaacgggg tggaaagaaa ttcccaaagg tggactaatt cttgaagcag
      961 gaaattctga acagtatgaa accggttcct ggcgttctca aagaccggtg tgggatgaac
     1021 aaaaatgtac ccattgtatg atttgctggg tctattgtcc cgattcttct attatagtag
     1081 aggagggaag ggttaagggt atcgattata aacattgcaa aggttgtgga atttgcgcag
     1141 ctgaatgtcc taccaaggct attgcaatga aagatgaaaa tgaatttcga gaaaaggagg
     1201 gagtataaaa atgagcatca agacggcagc agttactgga aatgaagcag cttcctatgc
     1261 tttaagacag attaatcccg atgtggtagc agcttatcct attactccac agactgaatt
     1321 agttcaaagt ttttctgaat acgtagcaga tggattggtc gatactgagt taatcacagt
     1381 agaaagtgaa cacagtgcta tgagtgcctg tgttggagcc gccgccgccg gtggccgagt
     1441 tatgactgct acttctgcca acggactggc tttaatgttt gaaatcctat ttattgcagc
     1501 ttccaataaa ttacctatcg ttatgccggt agtaaacaga gcactgtcag gaccaatcaa
     1561 tattcactgt gatcatggtg atactatggc agcaagggat gcaggctgga tacagattta
     1621 ttctgaaaat gctcaagaag tctatgataa cctgatacag gcgataagaa ttgcagagaa
     1681 tatgaaggta agattaccgg taatggtaat gtttgatgga tttatcgtat cacatggcat
     1741 tgaaagagtt gattatctgg atgatgctac tgtacgtaat tttattggta catatcagcc
     1801 aaatcacccc ttactcgatg tt
//
LOCUS       DAOQWT010000360         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_76995_length_1822_cov_2.449349, whole genome shotgun sequence.
ACCESSION   DAOQWT010000360 DAOQWT010000000
VERSION     DAOQWT010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76995_length_1822_cov_2.449349"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKJ95_06845"
     CDS             complement(<1..209)
                     /locus_tag="PKJ95_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65989.1"
                     /translation="MRVIVGHTSADFDCLAAMIAAKKIYTDALVVFPGAIEENVRKFL
                     LLYENALCISSIKTIDLEEITELIIV"
     gene            complement(304..1608)
                     /locus_tag="PKJ95_06850"
     CDS             complement(304..1608)
                     /locus_tag="PKJ95_06850"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNR65990.1"
                     /translation="MDIIEIIEKKKNGLILSEDEINFVVREYLNENIPDYQMSAFLMT
                     IWFKGMNYDETNHLTIAMARSGRIIDLSSIPGIKVDKHSSGGVGDKTTIALIPMVSAA
                     GVTVPKLSGRALGHTGGTIDKLESINNFITDLSIEKFIENIKRTKASIISASADISPA
                     DKKIYALRDVTGTTDSIPLMASSIMSKKIAGGADAFVLNVTVGEGAFLKKLSDAISLA
                     KIMVNVGKSNHKKTYVIISSMEQPLGYAVGNSLEVQEAIELLNNRGPSDLKELCFFLG
                     SYMLIAAGIVKKQIDGITLLQETIKSGKALNKFIEIVKNQNGQISQIKKPSLLPRAKY
                     HEDIYSFRKGFISKVQAKTIGQAAMLLGAGRKKKDEHIDLSVGIILKKKTGDPVKANE
                     TLATVYFNSVEKYSLIKESVQKSFHITDQKPEVKPLILKVIS"
BASE COUNT          604 a          333 c          250 g          635 t
ORIGIN      
        1 acaataatca gttcggttat ctcttctaaa tcaattgttt taattgaaga aatgcacagg
       61 gcattttcat ataacaaaag aaatttacga acattttctt caatagcacc tggaaaaaca
      121 accaaagcat cggtatatat ttttttagca gcaatcatgg ctgccaggca gtcaaaatca
      181 gcgctggtat gaccaacaat tactctcata aaatttaaaa ccactttaag tacaattaat
      241 ataatattta aaaaaatttt aaaaaatact tttagcagaa aagaaagaaa atagtggttt
      301 aaactagcta attactttta atattaacgg ttttacttct ggtttttgat cagtgatatg
      361 aaaggatttt tggacacttt ctttaatcaa actatatttt tccacactgt tgaaatagac
      421 agtagccagg gtttcatttg ccttaactgg atctccagtt ttctttttta atattattcc
      481 aacagataaa tcaatatgtt catctttttt ctttctacct gctcctaata acattgcagc
      541 ctgaccaatt gttttggctt gaactttact tataaaacct tttctaaaag aataaatatc
      601 ctcatgatat tttgccctgg gaagtaagga aggtttcttt atctggctta tttgaccatt
      661 ctgattttta acaatctcaa taaatttatt caaagccttc ccacttttaa tggtttcttg
      721 caacaaggta atcccatcaa tctgtttttt tacaattccg gcagcaatta acatatatga
      781 gcccaggaaa aaacataact cttttagatc agaaggtcct ctattattca gtaattctat
      841 agcttcttga acttctaaag agtttccaac ggcatacccc aatggttgct ccattgagga
      901 aatgattaca tatgtttttt tatggttact ttttccaaca ttgaccatta ttttagctaa
      961 agaaatagca tctgaaagtt tttttaaaaa agctccttct cccactgtca cgttaagaac
     1021 aaaagcatct gcaccaccgg caattttttt actcatgatg ctactggcca ttaatggaat
     1081 agaatcagta gttccggtaa catccctgag agcataaatt tttttatctg caggagaaat
     1141 atcggcactg gcacttataa tggaagcttt agttcttttt atattttcaa tgaatttttc
     1201 aatagatagg tcagttataa aattattgat cgattctaat ttatcgatgg tgcctccagt
     1261 atgtcccagg gctctgcctg atagttttgg aactgttacc ccagcagcgg agaccatcgg
     1321 tatcaatgcg attgttgttt tgtctcccac gccaccagag ctatgtttgt caactttaat
     1381 tcccggtata gagcttaaat caattattct tccagaccga gccatggcta tggtcagatg
     1441 attggtttca tcataattca tgcccttaaa ccaaatagtc ataagaaatg cagacatctg
     1501 atagtcggga atgttttcat tcaaatattc tctaaccaca aaatttattt catcttcact
     1561 taaaatcaaa ccattcttct ttttctcaat aatttcaata atatccataa taaaaatata
     1621 ttgttataga aaagaaattg aaaaatataa gctccttccc cactgtatta taaaattttt
     1681 gaagcatcac ctaaaaccta taatatttta atagtactaa tccataatta gaaaagtgtc
     1741 attgtataaa aatatataat tcttatccac cctaatttta gagatactat ataaagagtg
     1801 gaataaatac caaggccaca at
//
LOCUS       DAOQWT010000361         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_77426_length_1816_cov_3.015900, whole genome shotgun sequence.
ACCESSION   DAOQWT010000361 DAOQWT010000000
VERSION     DAOQWT010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77426_length_1816_cov_3.015900"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PKJ95_06855"
     CDS             <1..341
                     /locus_tag="PKJ95_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNR65991.1"
                     /translation="GDGINDAPALAQADVGIAIGSGTDIAAEIADIILVNSNPSEVFS
                     LILFGRATYRKMIQNLFWATAYNIIAIPLAAGVFYNSGIVISPAISAALMSLSTVIVS
                     INAQFLKVEK"
     gene            complement(389..802)
                     /locus_tag="PKJ95_06860"
     CDS             complement(389..802)
                     /locus_tag="PKJ95_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR65992.1"
                     /translation="MKKFYCFSVLFLILSLILSSCTGVTDGIDEELAPIESIDILVLE
                     SFPVQINVLINGYLPNPCYQITRIEKNQEGNTFYIKVFMKYNGLICIQTIKPYKETVA
                     LDVYGLVAGTYQVDVNGVVGSFTLNTDNILTDIEE"
     gene            1144..1788
                     /locus_tag="PKJ95_06865"
     CDS             1144..1788
                     /locus_tag="PKJ95_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNR65993.1"
                     /translation="MKKNHILIILIVITLLYFSSGAFAKTEEYFSLYDDPESIIIKNI
                     DNAEESINIAMYSLTDSDIAWAIVRANERGVVVKIYLDNKEVDAEHSKSRFFIKEGIE
                     NIRLSSNRYLMHNKFAIIDNKKIITGSYNWTASAGQRNDENLLILDEEEIVKKYQDYF
                     HYLWDNKYSVEKYNELINHSDIIPGVEKNPLKKFLVSLLAQIKSLKIIESFWKD"
BASE COUNT          640 a          259 c          296 g          621 t
ORIGIN      
        1 ccggcgatgg aataaatgat gcccctgctc ttgctcaagc cgatgtggga attgctattg
       61 gctcagggac tgatattgct gccgagatcg ctgatattat cctggtaaac agtaatcctt
      121 cagaggtatt ttcactgata ttgttcggaa gggcaacata ccgcaaaatg attcagaatc
      181 tattctgggc cactgcttat aatatcattg ccattccact ggctgctgga gtgttctata
      241 actcaggtat agtcatttct ccagcgatta gtgctgcttt aatgtctcta agcacagtta
      301 tcgtttctat caatgcacaa ttcttaaaag tagaaaagta atattaattt ttaattatag
      361 tttcctgaga agagaaatct atacctactt attcttcaat atcggtcaat atattatcag
      421 tattcagggt aaaagagccc acaactccat tcacatcaac ttgataagta ccggcaacaa
      481 gtccgtagac atccagtgct actgtttctt tataaggttt gatggtctga atacatatta
      541 agccattgta tttcatgaac acttttatat agaaggtgtt tccttcttga ttcttttcaa
      601 ttcgggtgat ttgatagcag gggttgggta aatagccatt gataaggaca ttaatctgta
      661 caggaaaaga ttctaagacc aatatatcaa tagattcaat aggggctaac tcttcatcaa
      721 taccatctgt tacacccgta caactactca gtatcagaga taaaattaga aacaaaactg
      781 agaaacaata aaattttttc ataaaaacat ctccttaaaa aggtatgcgt ggaaacgcat
      841 tttttcatgt tattatttta tcacaaattt aaatgataat agctatttat ctgtaataat
      901 gtaatatcct ttctttttta tatatgtttt tacgaagaat aaaattgtat ttgcttaccc
      961 aatttgttat tatcgaagta tatttgaaga atttaaatgt aacattttgc tcaattttca
     1021 ttagctgtat cagaaaaaca gtaaaaatag aattttgtat caaaactatg ttgtatactc
     1081 acataaagta tttttttatt attttgaagt tctaattatg tcttgttgta tagggaagta
     1141 taaatgaaaa agaatcacat tttaattatt cttattgtga ttacattatt atatttttca
     1201 tcaggagctt ttgctaaaac tgaggaatat ttttctcttt atgatgatcc ggaatcaata
     1261 ataatcaaaa atattgacaa tgcagaagaa tcgataaata tagctatgta ttcccttact
     1321 gatagtgata ttgcctgggc aatagtacga gccaatgaaa ggggagttgt tgttaaaata
     1381 tatttggata ataaagaggt ggatgctgaa catagcaaat ctcgtttctt tatcaaagaa
     1441 ggtatagaaa atattagact tagttcaaat agatatttaa tgcataacaa atttgccatt
     1501 attgataata aaaaaataat aaccggttca tataactgga ctgcctctgc agggcaaaga
     1561 aatgatgaaa acctccttat attagatgaa gaggaaatag taaaaaaata tcaggattat
     1621 tttcattatt tatgggataa taaatacagt gtagaaaaat ataatgaatt aattaaccat
     1681 tcagatataa tacctggggt tgaaaagaat cccttaaaaa agtttttggt atctttattg
     1741 gctcagataa aatcgttaaa aataatagag tcgttttgga aagattaaag atacataaat
     1801 aaaaaaagta ttttat
//
LOCUS       DAOQWT010000362         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_78012_length_1807_cov_3.410959, whole genome shotgun sequence.
ACCESSION   DAOQWT010000362 DAOQWT010000000
VERSION     DAOQWT010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78012_length_1807_cov_3.410959"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PKJ95_06870"
     CDS             complement(<1..433)
                     /locus_tag="PKJ95_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNR65994.1"
                     /translation="MLRLVLATGNRGKAIEIREKLKKYPLIIESLQDYPQIPEIEENG
                     KTFLDNSVLKASIVSRYTNLPALADDSGLEIDYLKGLPGLYSSRWGKDDRDRVARVLK
                     ELEGITEEQRKARFVCVMSMVVPGNKVYTEQGVCSGKITFSP"
     gene            complement(421..1176)
                     /gene="rph"
                     /locus_tag="PKJ95_06875"
     CDS             complement(421..1176)
                     /gene="rph"
                     /locus_tag="PKJ95_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNR65995.1"
                     /translation="MQKGDVEIMLRAFNRKYDEIRPIKIIRNYIKYSEGSALIEMGDT
                     RVICTASVEEKVPAFLRGTQQGWISAEYSMIPRANQVRNIRDSVKGKIDGRTQEIQRL
                     VGRSLRAVTNLEKLGSRMIWMDCDVLQADGGTRSASIIGSFIALIDALQYLKAEKKIE
                     NIPIKHFLGAISVGIVDGEMLLDLDFKEDSQAQVDMNLVMTERGDVVEIQGTAEDRPF
                     SMTNLSKLIKLAEIGMNQVISIEREIIGELCSG"
     gene            complement(1187..>1807)
                     /locus_tag="PKJ95_06880"
     CDS             complement(1187..>1807)
                     /locus_tag="PKJ95_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNR65996.1"
                     /translation="LVHDDLPCMDDDDYRRGKLTNHKVFGEGIAVLTGDALLVMGLDF
                     IFQNAQVEGISKESVLQVIGKVLEMLGTRNMLGGQSDDINWKFQKQIQNKIVEIYLKK
                     TSALICASLETGALLSQANEKEIEALHTFGKYFGLAFQITDDLLDFQQDKKGDEKPSY
                     PAVFGIEKAKDEIMKYCLQAKDSLGIFGSKAELFLELVDYFQHRKE"
BASE COUNT          505 a          451 c          267 g          584 t
ORIGIN      
        1 gtggagagaa agtaattttt ccactgcata caccctgctc agtatagacc ttatttcccg
       61 gaactaccat actcataaca cagacaaaac gggcttttct ttgctcttct gtaatcccct
      121 ccaattcctt taaaaccctt gctactctat cccggtcatc cttcccccat cgggaggaat
      181 aaaggccggg caatcccttc agataatcaa tttccaaacc cgaatcatct gccagagcag
      241 gtaaattagt ataacgcgaa actatacttg ctttgagaac tgaattatct aaaaatgttt
      301 taccattttc ttcgatttca ggaatttggg gataatcctg tagagattct attattaatg
      361 ggtatttttt taacttttcc cgtatttcta tggcctttcc cctattgcca gtggcaagaa
      421 ctaacctgag cataattcac ctattatttc tctctcaata gaaatcacct gattcattcc
      481 aatttcagcc agtttaatca atttacttaa attggtcata gagaaaggcc ggtcttcagc
      541 agttccctga atttcaacta catcccctct ctctgtcatt accagattca tatccacctg
      601 agcctggctg tcctctttaa aatccaaatc cagtaacatc tctccatcca caatccccac
      661 acttatagct cctaaaaaat gctttatagg gatattttca atcttttttt cagctttcaa
      721 atattgaagg gcatctatca gagcgatgaa agaaccgata atggatgcag agcgggttcc
      781 accatctgcc tgcaatacat cacaatccat ccagatcata cgggaaccaa gtttttctaa
      841 gttggttacc gctcttaacg agcgtccaac caacctctgt atctcctgag tcctaccgtc
      901 tattttccct tttacggaat ccctgatatt tctcacctga tttgcacggg gaatcataga
      961 atattcagct gaaatccagc cctgttgtgt gcctcttaaa aatgcgggca ccttttcctc
     1021 cacgcttgcc gtacaaatca ctctggtatc tcccatttct attaaggcag aaccctcaga
     1081 atatttaata taattacgta ttatttttat tggcctgatt tcatcatact tgcgattaaa
     1141 agctcttagc atgatttcta catctccttt ttgcacaatg cagcttttac tctttgcggt
     1201 gctggaaata atccaccagc tctaaaaata actctgcctt gcttccaaat attcctaaac
     1261 tatcttttgc ttgcaggcaa tatttcataa tttcatcttt agccttttca ataccaaaaa
     1321 ctgccggata agacggtttt tcatcacctt tcttgtcctg ttgaaaatca agcaaatcat
     1381 cggtaatttg aaaagcgagg ccaaaatatt taccaaaggt atgcagggct tcaatctctt
     1441 tctcattagc ctgagataat aaagctcctg tttccaaaga tgcacagatt aaagccgaag
     1501 ttttttttaa atatatctcc actattttat tttgaatttg tttttggaac ttccagttaa
     1561 tatcatcact ttgaccgccc agcatattcc tggttcccaa catttcaagc acctttccta
     1621 tcacctgaag aactgattct ttgctaatac cttctacctg tgcattctga aagataaaat
     1681 ccaggcccat taccagcagg gcatcaccgg ttaatacggc tataccctct ccaaaaactt
     1741 tatggttggt taactttccc cgacgatagt catcatcatc catacagggc aaatcgtcgt
     1801 gaaccag
//
LOCUS       DAOQWT010000363         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_79254_length_1790_cov_2.659942, whole genome shotgun sequence.
ACCESSION   DAOQWT010000363 DAOQWT010000000
VERSION     DAOQWT010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79254_length_1790_cov_2.659942"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PKJ95_06885"
     CDS             <1..803
                     /locus_tag="PKJ95_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR65997.1"
                     /translation="KNSFHGRTIATVAATGTYKYQKDYLPLLPSFKEAIFNDLQSVKD
                     VYDEKVAAIIVEPIQGEGGINIGTEDFLEGLREFCDNKDILLIFDEIQCGLGRTGKMF
                     AYQHYGVEPDIMTLAKPLAGGLPLGAMIAKEKVASVFKPGDHGTTFGGNPVACAAALA
                     FLEVLQEDNLLMECQLKGEYFQEKLEEVEEKFPALVKEIRVIGLMVGLELTREGSAIV
                     EKMLKKGFLINCTAEKVLRFLPPLIVQKEEIDLLVKTLEEVVTEIVFS"
     gene            complement(1062..1538)
                     /locus_tag="PKJ95_06890"
     CDS             complement(1062..1538)
                     /locus_tag="PKJ95_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNR65998.1"
                     /translation="MLLYYIFSYSYITGFYELNKIDTFSNFILGQFKEAVILGTLCTI
                     GMTILRLPYPTTIGSVVGLSSVIPMVGSYIGATFGILLIIVVDWVSAVFFLVFIIILQ
                     QIESNFIYPKIVGDSIGLPGIWVFAAIIIGSGLMGFLGILLGVPIAATIYKLLNHY"
BASE COUNT          612 a          283 c          351 g          544 t
ORIGIN      
        1 tgaaaaattc ctttcacggc agaaccattg ctactgtggc agcaaccggg acctacaaat
       61 atcagaaaga ttatttgcca cttttaccat cttttaaaga ggctattttt aatgatttac
      121 aatcagttaa agatgtttac gatgaaaaag tagctgcaat catagtggag ccgattcagg
      181 gtgagggcgg tatcaatatt gggacagagg attttttgga agggttaagg gagttttgtg
      241 ataacaaaga tatactctta atctttgatg agatacaatg tggcctgggc agaacaggaa
      301 agatgtttgc ctatcagcat tatggagttg aaccggatat tatgacttta gccaaaccat
      361 tggctggagg cttgcctctg ggtgccatga ttgccaaaga aaaagtagcc tcagtattta
      421 agccaggtga ccatgggacc acattcgggg gtaatccggt agcctgtgct gctgcactgg
      481 cttttttaga agttttgcag gaagataacc tcttaatgga atgtcagcta aaaggggaat
      541 actttcagga gaaattagaa gaggtggaag aaaaattccc tgctttagtc aaagagataa
      601 gagtaatagg attaatggtc ggtttagagc ttaccagaga aggttcggca attgttgaaa
      661 agatgctaaa gaaaggattt ttgattaact gtacagcaga gaaggtattg cgtttcctac
      721 cgccactaat tgtacagaaa gaagaaatag acttgcttgt taaaacttta gaagaggtag
      781 taactgaaat tgtcttttct taaaggtaat ttactatcag ggggcagctg tactagctta
      841 ataaattggt aatttttatt agtttattat aaagctgtat agcctagttc ccaaatcttc
      901 ctaaatggtt attataatta gaatatttga tatcatctgg tggtcttatt taaaggtaga
      961 aagattgaaa gaaaacagag gaataaattg gacctctgtt gactgaatta aatcttttca
     1021 atcaactaaa cacttttaca gcaatatcat gattaattat tttaataatg attgagtagt
     1081 ttatagattg ttgctgcaat gggaactcct aataaaattc ctaaaaatcc cattaatcct
     1141 gagcctataa taatggcagc aaatacccat attcctggaa ggccaataga atcaccaact
     1201 atttttggat aaatgaagtt gctttcaatc tgctgtaaaa taataataaa tactaagaaa
     1261 aatactgcac ttacccaatc aacaactata attaaaagta tcccgaaagt tgccccgata
     1321 taagatccca ccattgggat aacagaggat aaccctacta cagagccgat agttgtcggg
     1381 taaggaagcc ttaatattgt catgcctata gtgcataggg tgcccaatat gacagcttct
     1441 ttaaattgtc caaggataaa attagaaaat gtatcaattt tatttaattc ataaaagcct
     1501 gtaatatagg aataggagaa aatatagtat agtagcaaaa ccttatagca agcagctttc
     1561 agtaaagatg attatcattt tcgagctatc ttaactaaag gaaaagccac tcataaaagg
     1621 tgacttttcc ttttctcctc aaccagtaga gtagaggact gttgttaaat agcaaattgt
     1681 ttactcttta tttttcaaca agaaatgaaa ctttagcatc aacccgatat ctggcaattt
     1741 cattattttc tactattgcc tgaaaacttt caatgtatac agcttggata
//
LOCUS       DAOQWT010000364         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_79321_length_1789_cov_2.749135, whole genome shotgun sequence.
ACCESSION   DAOQWT010000364 DAOQWT010000000
VERSION     DAOQWT010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79321_length_1789_cov_2.749135"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1108)
                     /gene="pdxS"
                     /locus_tag="PKJ95_06895"
     CDS             complement(224..1108)
                     /gene="pdxS"
                     /locus_tag="PKJ95_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNR65999.1"
                     /translation="MVDLSREKAKRDFAQRFKGGVIMDVVTAEQAKIAENAGAVAVMA
                     LERVPADIRAAGGVARMADPIKVKEIMAAVNIPVMAKVRIGHFVEAQVLEALGVDYID
                     ESEVLTPADEKYHINKYNFKVPFVCGARNLGEALRRIAEGAAMIRTKGEPGTGNIVEA
                     VRHMRCVNEEIRALKGLDKEELIVKAKEYGAPFDLLLETASLGRLPVVNFAAGGIATP
                     ADAALMMQLGCDGVFVGSGIFKSDDPEKRAKAIVEATAHYKDPKIIARVSEGLGEAMK
                     GIAIDKIPEEEKMSVRGW"
     gene            complement(1367..>1789)
                     /locus_tag="PKJ95_06900"
     CDS             complement(1367..>1789)
                     /locus_tag="PKJ95_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907413.1"
                     /note="catalyzes the formation of acetyl-CoA from pyruvate
                     and coenzyme A; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HNR66000.1"
                     /translation="QYRKDLTGIIAAHNIPYAAQASIWRWNDLIDKAHKAFYTKGPAF
                     LNVLSPCHRGWRFPLEDTINISKLAVQTNFWPLYEVENGKYKLNYKPKERLPITEWMK
                     PQGRYKHILTPENKSIVEEIQQEIDNNWERLLVLSGEK"
BASE COUNT          481 a          418 c          269 g          621 t
ORIGIN      
        1 tatctggcga taattaactc cccttttagg tgacattttc taaagttatt gacagaaaat
       61 attttaattt tttttggata tttttagcaa taaaattact gtgattttag aatgttttgc
      121 agtttggtat agaaatcaat taaccataga attgatgtat atataatact tcaatttatt
      181 ttatttgttt tatttatttt ctaaaacttc ctgcttcccg taattaccag cctcttaccg
      241 acatcttctc ttcttcagga atcttatcta tagcgattcc cttcattgcc tcacccaatc
      301 cttctgatac tctggcaata attttggggt ccttataatg ggctgtcgcc tcaacaattg
      361 cttttgctct cttttcaggg tcatctgatt taaaaatacc tgaacctaca aatacaccat
      421 cacatcccaa ttgcatcatc agtgctgcat ctgccggtgt tgcaatacca cctgcagcaa
      481 aattgactac cggcaatcgc cctaaggacg cggtttccaa aagcaggtca aaaggagcac
      541 catattcttt ggccttgaca atcaattctt ctttatccaa tccctttaat gctctaatct
      601 cctcattcac acaacgcata tgtcttactg cttcaacaat atttccggta ccgggctctc
      661 ccttagttct aatcattgca gcaccttctg cgattctcct caaggcctct cctaaatttc
      721 tggcaccaca aacaaaggga accttaaaat tatatttatt gatatgatat ttttcgtctg
      781 ccggggtgag aacttcactt tcgtcaatat aatcaacacc cagtgcttct aatacctgtg
      841 cttcgacaaa atgtccaatt ctaacctttg ccatcaccgg tatattaacg gctgccataa
      901 tttccttaac cttaataggg tctgccatac gcgctacacc tccggcagca cgaatatcag
      961 ccggaactct ctctaatgcc atcactgcta ctgcaccggc attttcggca atcttagcct
     1021 gttccgctgt taccacatcc ataattacgc cgcctttaaa cctttgggca aaatcccttt
     1081 tagccttttc cctggataag tctaccactt tctatccctc ctggtcttct tatttgttag
     1141 aaacctcatt ggtatcttct actaatatca ttatataata cttgtaaacc ctatttcaag
     1201 aagaaataga ttttaagaaa atagactgct ctaagcagaa gaaaaaatta gaacagtcta
     1261 ttttccaagc aattctctat tcccatgttc ctctgaggaa gcaaggaaat tactctattc
     1321 ttatttctta attcttaatt tctataattt tttatcgctg attttattac ttctccccac
     1381 taagaacaag cagtctttcc cagttattat caatttcttg ctgaatctct tctactatcg
     1441 atttgttctc cggggtaaga atgtgtttat atctgccctg tggtttcatc cactcagtaa
     1501 tgggcagcct ttcttttggc ttataattca atttatattt gccattttcc acttcataca
     1561 atggccagaa attggtctgg acggcaagtt ttgatatatt aattgtatct tccaggggga
     1621 aacgccatcc tctatgacag ggtgaaagaa cattcaaaaa ggcaggtcct ttggtataaa
     1681 aagctttatg cgctttatct attaggtcat tccagcgcca gattgaagcc tgtgccgcat
     1741 aggggatatt atgtgccgca ataatcccgg ttaaatcttt tcggtattg
//
LOCUS       DAOQWT010000365         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_79477_length_1787_cov_2.360855, whole genome shotgun sequence.
ACCESSION   DAOQWT010000365 DAOQWT010000000
VERSION     DAOQWT010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79477_length_1787_cov_2.360855"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1048)
                     /locus_tag="PKJ95_06905"
     CDS             complement(146..1048)
                     /locus_tag="PKJ95_06905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNR66001.1"
                     /translation="MDKEVTFFLKYSDKSNFFIKILLIFILFLIFTLSGYAERDYEKE
                     EKIGRRLAESVEKQYKLIEDDEAIRKVSQIGEYLKEASGINKINYQIKIVEREGPNAF
                     ALPGGFIYLTADLLKYVHSDDELAAVIAHEMGHIIHQHSIKQLQDKQKLKLVELLTIL
                     VTGDSTLGILSELASITILNFYRREYEEEADFTALELLQKSSHYHPVALLTYFERVSS
                     EDMLKPDINLGIFKTHPDVNERIEKIKQYLNEHNIPLNRRLTTNYLTVRGDYYQENIM
                     FIAQIMINDELILSFAGEDGKYLF"
     gene            complement(1078..>1787)
                     /locus_tag="PKJ95_06910"
     CDS             complement(1078..>1787)
                     /locus_tag="PKJ95_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNR66002.1"
                     /translation="KEKSTLRIKNEQLEELRIIEIERFDKSACGGTHCHRTGEVGLIK
                     IIGWENRKDKIRLSFLCGYRALTDYQQKHHIVKKLSHYFTTGVDQLEEKIIQLNEEQK
                     ELTKLYNKMEKRIIEGESEELKDKNRRKEKVFFLIEKLFMEQKIQNIRQIAQLLAKDE
                     KTITILGAEKPEPVLCLTLSSDLNYPIKEIFNQVMFEFGGKGGGAKHFVLGKLEREED
                     VKQAYQRAIQLILSLEK"
BASE COUNT          511 a          365 c          256 g          655 t
ORIGIN      
        1 tctttccgga ttaaagacac tacctcttca ccacctatgt taagtgtggc ataatcatca
       61 ttttcagagc tatttattac caattcatat ggtgccagat ctaatcttaa ggagtgatta
      121 aaattggcaa gtacctcgta catttttaaa ataagtactt cccatcttcc ccggcaaagg
      181 ataaaatgag ctcatcatta atcataattt gagcaataaa cataatattt tcctggtaat
      241 aatcacccct caccgttaaa taattggtag taaggcgacg attcaggggt atgttgtgtt
      301 cattgagata ctgtttaatc ttctcgattc tctcattaac atcagggtgc gttttaaaaa
      361 tgcccaaatt tatatctggc tttaacatat cttcgctgct cacccgttca aaataggtca
      421 ataatgctac cgggtgataa tgagaacttt tttgcagtaa ttccagggcg gtaaaatctg
      481 cttcttcttc atattctctt cgatagaaat tgaggatagt gatgctggct aactcactta
      541 aaatacccag tgtagaatcc ccggtaacta atatggtaag caattcaacc aattttaatt
      601 tttgtttatc ctgtaattgt ttgatggaat gctggtgaat tatatgcccc atctcatgtg
      661 caataaccgc tgccagctca tcatctgaat gcacatattt caataaatct gccgttaaat
      721 agatgaatcc gccaggaagt gcaaaagcgt taggcccttc cctctcaaca attttaatct
      781 gataattaat cttatttatt cctgatgcct cttttaaata ctctcctatc tgtgaaacct
      841 tcctgatagc ctcatcatct tcaatcaatt tgtattgctt ctctacactt tctgccagcc
      901 gccgtcctat tttttcttct ttttcataat cccgctcagc atagccgctt aaggtaaata
      961 ttaaaaatag gataaaaatt aataatattt taataaaaaa attactctta tcagaatatt
     1021 ttaaaaagaa ggtaacttct ttatccataa atatattatt tacctgttct aaattaatta
     1081 cttttcaagg gatagtatta actgaattgc cctttgatag gcttgtttaa catcttcttc
     1141 tctttccagt tttcccagaa caaaatgttt ggctcctcca cctttacctc caaactcgaa
     1201 catcacctgg ttgaatattt ctttaatagg gtaatttaga tcagaagaga gggttaaaca
     1261 caataccggt tcgggttttt cagctcccag aattgtaatg gtcttttcat cctttgccag
     1321 gagttgggca atttgcctga tattttgaat tttttgttcc ataaataatt tttcaataag
     1381 aaaaaaaacc ttttcttttc tgcgattttt atctttcagc tcctcactct ctccttctat
     1441 tattcttttc tccatcttgt tatataattt agttagttct ttttgttcct catttaattg
     1501 tataattttt tcttctaatt gatctacccc agtagtaaaa taatgggaga gttttttaac
     1561 aatgtggtgt ttttgttgat agtcagttaa agccctgtaa ccgcacagaa aagaaagcct
     1621 tattttgtcc ttacgattct cccagccaat aattttaata agccccacct ctcccgttct
     1681 atggcaatga gtacctccac aggcagattt gtcaaaccgc tcaatctcga tgattctcaa
     1741 ttcttccaat tgttcatttt taattctaag ggtacttttt tctttca
//
LOCUS       DAOQWT010000366         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_79682_length_1784_cov_3.665703, whole genome shotgun sequence.
ACCESSION   DAOQWT010000366 DAOQWT010000000
VERSION     DAOQWT010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79682_length_1784_cov_3.665703"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1437
                     /locus_tag="PKJ95_06915"
     CDS             181..1437
                     /locus_tag="PKJ95_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HNR66003.1"
                     /translation="MSEYSNIKEKEKAQNIALFRHGIISLVMHEGEESQNQILKKMAE
                     KEHFIQGVGPKKFSVSTFKSWLAAYREGGYNGIKPRIRVDKGSSRKIDGDFTEIINKL
                     LQEIPITTYTLLYRQLIEKGYIRPGDFTLQTLIKFLKDNNISLCQREVVPRKKFETEE
                     INKLWICDFMHSIKLWDGKKKKFTYLCAIIDDHTRVITGSNWSFEENLIVLEKTFKEA
                     ILSYGKPQKFYCDNAKVFTSEAIHLPCAKLGIALIHSKPYVPCGRGKIERFFRTVQSA
                     FIPTVKERNFSITELNSSFQEWIRTQYHRNIHQGLGEPPMDRLLREFDSVKIERIPEE
                     TLDLIFYRTIKRTVKLDCTVSINGKLYEAPHKYIGLQVELRYPSSSPQEIYIFEQDKP
                     VYKLKPLNLQQNANQPFVSFSYSNLF"
     gene            1450..>1784
                     /locus_tag="PKJ95_06920"
     CDS             1450..>1784
                     /locus_tag="PKJ95_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR66004.1"
                     /translation="MFFNFYNLKSIPFEKSIKKDNLFLSASLKEFFSRMEYLKQFKGL
                     LLLTGEAGVGKTTAIRAFLEDLKQEYFKFIYIPLTTVSTIDFYHQLNSSLGGESKRNK
                     SELFSSIQK"
BASE COUNT          634 a          302 c          290 g          558 t
ORIGIN      
        1 attaatttat tattatttct tcttttcccg tgttaaaaat acttcttgat ataaaaattt
       61 attaattttt tgactgaaga aataaaaatt ttataatttt gtgcaccttc acaccaggct
      121 atatataaga ttttctctga aaattttata atttatttta acattattgg aggtgaacat
      181 atgagtgaat actcaaatat taaagaaaaa gaaaaagccc agaacatagc attattcaga
      241 catggaataa tctcccttgt aatgcatgaa ggagaagaaa gccagaacca gatcctgaaa
      301 aaaatggcag aaaaagaaca tttcatccag ggagttgggc cgaaaaaatt ctccgtatca
      361 acatttaagt cctggctcgc tgcctacaga gaaggaggct acaatggaat aaaaccaaga
      421 ataagggtgg acaaaggttc atcaagaaaa attgacggcg attttactga aataataaac
      481 aaattattgc aggaaatccc tataacaacc tatactctcc tttacaggca gcttatagag
      541 aaagggtata taagacccgg tgattttact cttcagacgt taatcaaatt cctgaaagac
      601 aataatatat ctctctgtca gagagaagtt gttcccagga aaaaattcga aacagaagag
      661 ataaataaac tctggatatg tgatttcatg cacagtataa aactatggga tggaaaaaag
      721 aaaaaattta cttatctctg tgccatcata gatgatcata caagggttat cacaggaagt
      781 aactggagtt ttgaagaaaa ccttattgtg ctggaaaaga catttaaaga agcaattctt
      841 tcatatggca aaccacagaa attttactgc gacaatgcaa aggtttttac atctgaagca
      901 atacatcttc cttgtgctaa actgggcatt gcacttatcc attctaagcc ttatgttccc
      961 tgtggtcgtg ggaaaataga acgctttttt cgtaccgttc aatcagcttt tattcccact
     1021 gttaaggaac gtaatttctc tattacagag cttaattcct cttttcagga atggataaga
     1081 acacaatacc atagaaatat acatcagggg ctcggagagc ctcctatgga cagactgtta
     1141 agggaatttg actctgttaa gattgaaaga atccctgaag aaacacttga tttaattttt
     1201 tataggacta ttaaaagaac tgtaaaactt gattgtactg tttctatcaa tggcaaactc
     1261 tatgaggctc cacataaata cattggtctt caggttgaat tacgctatcc ttcttcttca
     1321 ccacaggaaa tttatatttt tgaacaggat aaaccggtat ataaacttaa accgcttaac
     1381 ctgcaacaga atgcaaatca accttttgta agtttttcat attctaatct tttttaggga
     1441 gtgatacatt tgttttttaa tttttataat ctcaaatcaa tacctttcga aaaatctata
     1501 aaaaaagata atttgtttct ctccgcttct ttaaaagaat tcttctccag aatggagtat
     1561 ttgaaacaat ttaaaggcct cctattactt actggagaag caggagtagg aaagactact
     1621 gctatcagag ctttcctgga agatttaaaa caggaatatt ttaaattcat ttatattcct
     1681 cttactactg tttctaccat agatttttat catcagctta acagctctct cggcggagaa
     1741 agtaagagaa acaaatctga actattttca tctatacaga aaat
//
LOCUS       DAOQWT010000367         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_79821_length_1782_cov_3.298205, whole genome shotgun sequence.
ACCESSION   DAOQWT010000367 DAOQWT010000000
VERSION     DAOQWT010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79821_length_1782_cov_3.298205"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            409..849
                     /gene="mraZ"
                     /locus_tag="PKJ95_06925"
     CDS             409..849
                     /gene="mraZ"
                     /locus_tag="PKJ95_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNR66005.1"
                     /translation="MFLGQYEHSLDKKSRIIMPAKFRENLGDSFIITFGLEKCLFVYP
                     MDEWEKLSQNLQHLPLGKKDTRAFKRTLASRASVHSFDQQGRAVIAKYLRDYAGIEKN
                     VVIIGVFERIEIWDSEKWTDYARETEQSYEEIAERVYEKEGQDK"
     gene            849..>1782
                     /gene="rsmH"
                     /locus_tag="PKJ95_06930"
     CDS             849..>1782
                     /gene="rsmH"
                     /locus_tag="PKJ95_06930"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNR66006.1"
                     /translation="MAFHIPVMIEQVVTNLITRKTGIYVDCTVGGGGHSQNILETIYP
                     EGILVGLDQDIEALEFARERLKPYQDRVVLVKSNFADLAIVLQNLGFSKVTGVFFDLG
                     ISSHQVDTPQRGFSFNKDNFLDMRMDLSKKLHAHYIVNHYSEKQLTEIFSKYGEERFS
                     RSLARLIALERKKNPIETTNQLAELIINFYAKHKKGKWRIHPATKIFQAIRIEVNREL
                     SSLEEALWKAVNILEVRGRIGVISYHSLEDRTVKNIFREWEKKEIINQYGYGLKKLFK
                     KPIYPSEEEIKQNPRARSARLRLAEKIGTKEVSEK"
BASE COUNT          629 a          270 c          366 g          517 t
ORIGIN      
        1 tagctgcccg ctataagttt tcgggtagat cgctagaagt ataaggtaac ttatactcca
       61 gatagatgat taccacactg ttttatttca ttataaatac agtgacagaa ctcggcttat
      121 gatttacttg ccaatttaag tttaatcaga taaattctat ttagcctgat gcatccacgt
      181 taagatgggt gcttttttat tttataatca aaattgcatt tttcttcagg tttctatcag
      241 ctcagatatg cttttatccc atttttattt ttcaaataaa aactcctgaa taattcgata
      301 ttgaaaaaat caaaaaaata ttaaaaaatt tttaaaaaag gtgatttttt ttaaaaaatg
      361 tagtatacta agctcaaagt ggagggaaat ggaggaaaat ggtaaaaaat gtttctgggg
      421 caatacgaac actccttaga taaaaaaagt agaatcatta tgcctgctaa attccgggag
      481 aatttaggag actcatttat tataaccttt ggtttagaaa aatgcctctt tgtctatccc
      541 atggatgaat gggaaaaact ctctcaaaac ctccagcatt tacccctggg taagaaggat
      601 acccgagctt ttaaaagaac attggcttcc cgggcatctg ttcatagttt tgatcaacag
      661 ggaagagcag ttattgctaa atatttgagg gactatgccg ggatagaaaa gaatgttgta
      721 attattggag tatttgaacg tattgagata tgggactcag aaaaatggac agattatgcc
      781 cgggaaacag aacaatcata cgaggaaata gctgaacgcg tctatgagaa agaaggacag
      841 gataaataat ggcttttcat attcctgtaa tgatagagca ggttgttacc aatctgatta
      901 ccagaaagac aggaatatac gttgactgta ctgtgggagg aggcggtcat tcccaaaata
      961 tcttagaaac aatttatccg gaaggaattc ttgttggatt ggaccaggac atagaagcac
     1021 tggaatttgc ccgggaaaga ttgaaaccct atcaggatag agtggtgctg gtaaagtcta
     1081 actttgccga tttagcaata gttttacaaa atttgggatt cagtaaagtt acaggtgttt
     1141 tttttgacct gggaatttct tcccatcagg tagatacccc acagagaggg ttcagtttta
     1201 acaaagataa ttttctggat atgagaatgg atttaagcaa aaaattacat gctcattata
     1261 ttgtcaacca ttattcggaa aagcaattaa cggaaatatt cagcaaatac ggggaagaac
     1321 gtttttctcg ttctctggcc agattaattg cactggaaag aaaaaagaat cccatagaaa
     1381 caaccaatca gctggctgag ttgattatta atttttatgc caaacataag aagggcaaat
     1441 ggcgtataca tccagccaca aagatttttc aggctatcag gattgaggta aacagagagt
     1501 tatcctcatt agaggaggcc ctctggaaag cagtaaatat tttagaagtg aggggcagaa
     1561 taggggtaat atcctatcat tccctggaag atagaacagt aaagaatatc tttcgggaat
     1621 gggagaagaa agaaataata aatcagtatg gttatggctt aaaaaaatta tttaaaaaac
     1681 ccatttatcc ttcagaagaa gagataaaac aaaatcccag agctcgtagt gccagattga
     1741 ggttagctga gaaaataggt acaaaggaag tatctgaaaa at
//
LOCUS       DAOQWT010000368         1776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_80276_length_1776_cov_3.270192, whole genome shotgun sequence.
ACCESSION   DAOQWT010000368 DAOQWT010000000
VERSION     DAOQWT010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80276_length_1776_cov_3.270192"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PKJ95_06935"
     CDS             <1..806
                     /locus_tag="PKJ95_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNR66007.1"
                     /translation="SELPLSKGLRTEIGKEIENPFAAVIQIEGGNKDLSAYQSKIEYF
                     FHTLEEQEVSLLAFSQPGTAIPAPKSQDILELLKKLTKGTAQLCSIVLEEEENGGKYK
                     TIFLIQAGNIIGKYRKTHLKFQEKDKILPGEDGFPVFETQFGNIGMLLDYEGYFPEIS
                     RILALKGADIIIWTSHFSNDEHLKICQTRSAENKVFIICPNSIGEGCNGHSVITSPSG
                     QIIAGCLEGRKIASPATISVRLARDKMIVPYTNAILGRQPESYYLLTSE"
     gene            1374..>1776
                     /locus_tag="PKJ95_06940"
     CDS             1374..>1776
                     /locus_tag="PKJ95_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HNR66008.1"
                     /translation="MKAELIINHTAGGGKPEKYISEILSYFDRLNFEYHVSWTPSSGS
                     ATALARQAAEQGADLIISVGGDGTINEIINGIIQSSTQPALGIIPAGWANDFIKSTSI
                     PRDILQACQIISRKKTRMIDVGLINEETYFAN"
BASE COUNT          614 a          295 c          366 g          501 t
ORIGIN      
        1 ccagtgaatt gcccctcagt aagggactta gaacagagat tggtaaagag atagaaaatc
       61 cctttgctgc cgtaatacag atagagggag ggaacaaaga tttatctgca taccagtcta
      121 aaatagaata cttttttcat accttggaag aacaggaagt aagcctgctt gctttttctc
      181 aaccgggtac cgctatccct gccccaaaaa gtcaggacat actggaatta ttaaaaaaat
      241 tgaccaaggg taccgctcag ctctgttcta ttgtcttaga ggaagaggaa aatgggggaa
      301 aatataaaac catttttctg atacaggcag gaaatataat tggaaaatat aggaagacac
      361 atctgaaatt tcaggaaaaa gataaaatcc ttcctggtga agatggtttc ccggtatttg
      421 aaacacaatt tggtaatatc ggtatgctcc ttgattatga gggctatttt ccggaaattt
      481 cccgcattct ggctttaaaa ggagctgaca taattatctg gacaagccat ttcagtaatg
      541 atgaacatct gaaaatatgt caaaccagaa gtgcggaaaa caaagtcttt ataatctgtc
      601 ccaattctat tggagaaggt tgtaatggtc acagtgtgat cacctcacca tcgggacaga
      661 ttatagccgg ttgcctggaa ggaaggaaaa tagcaagtcc ggcaacaatt tctgtcaggc
      721 tggcaagaga taaaatgatt gttccataca ctaatgccat tctgggtcga cagccagaat
      781 cttattattt gttgacctct gagtaagcac agttttagtc gtttgtcgtt agtattaagt
      841 cgttagaata tattgtagtt tttggttaaa ataataagca actgtatagc gggctttacc
      901 ctaagaattg aaaacataaa gctcatagtt tatagttttt tgtttttggt aaaaaacaga
      961 gacaaagaca aagaaagagt gctggctata gggtatggga ttgccaggac atgcgttcag
     1021 catgtcttgt aaagacaggt gcaaagaaat aaaaacttat tttaaactaa aaaagataaa
     1081 acaaatatag ttgctgttat ttgtcgtcag caccattttc tgtggaaata aatgaaagta
     1141 taaggtttat agttaaaaat ttttagccta tagtgaagaa tagactcggt ataaaataac
     1201 taataaaaat atcccaaata catagactct taacaaaaaa ctaaaaacaa aaaactttat
     1261 agctaaaaac caacaactat tttgaattat acacttttta taaatgcgat tcgtgtaatg
     1321 tttttatagt gtaagattgt aatatagtaa taggataagg gggggggtaa accttgaaag
     1381 cagaactgat tatcaaccac accgccgggg gaggaaaacc tgaaaaatat atttctgaaa
     1441 ttttgagcta ttttgacagg ctcaattttg agtatcatgt cagctggaca ccctcttcgg
     1501 gaagtgcaac tgctctggca cggcaggcag ccgaacaggg ggctgattta atcatatcag
     1561 ttggtggaga tggtactatc aacgaaatta tcaatggaat tattcaatct tcaactcaac
     1621 cggctctggg cattatcccg gcaggatggg ctaatgattt tatcaaaagc acttccattc
     1681 ccagggatat cttgcaagcc tgtcagatca tctccagaaa aaagaccaga atgatagatg
     1741 taggccttat taacgaagag acatattttg ctaatg
//
LOCUS       DAOQWT010000369         1772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_80603_length_1772_cov_2.347699, whole genome shotgun sequence.
ACCESSION   DAOQWT010000369 DAOQWT010000000
VERSION     DAOQWT010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80603_length_1772_cov_2.347699"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..>1772)
                     /locus_tag="PKJ95_06945"
     CDS             complement(207..>1772)
                     /locus_tag="PKJ95_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNR66009.1"
                     /translation="AIVIRGIAKKPCYLWIKDGDVQFKDAAHLWGKETHQVDRLIKEE
                     FSDEKVRICQIGIAGENKVLYANIVNELTHFNGRNGLGAVMGSKKLRAIVVKGNIPIT
                     LYDSDKVKGIAKDISQRVMENPLSRALREYGTPATVRGFYEAGSLPSFNWTTGYFKGG
                     ENLTVEEYNKTILKKKGSCYACPIRCKRIVEVNGEEFTVNPVYGGPEYETLVAFGSLC
                     GVDDLQYVAKANELCNKWGLDTISTGAVIAFAMECYEQGLLNKKDTDGLELYFGNKKA
                     LLVLIEKIARREGFGELLAQGSRLASKRVGKGSDRFLKEVKGQEVPMHDPRVKAGLAL
                     QYAFSDYGADHMKAPHDAFFSEKESIGLKEMSGLGILKPVAVTDLSYRKVALYKILDI
                     YWSIFDILGVCDFGYVPRSLGTMDELLAIIRATTGWNTTWYELMKLGERTINMARIFN
                     IREGFTRDDDTVPEIFFTDFKEGPLKGSGALKREDFEEAKKLRYELMGWDKEGKPGRG
                     KLIELDLEWLIED"
BASE COUNT          525 a          426 c          254 g          567 t
ORIGIN      
        1 atgaagacat tgaagaattt gctaaaaatc tggcacagtg tatgatgtcc cagtaaaaat
       61 aaggaattga cagcatataa gcaatgacag ttaaattata atcacgctca tttgagattt
      121 gatgaaattg tttcagatga gcgtggttta ttaataaata ttttagcaat aatggtattt
      181 catataaatt actattgatt caatcttcaa tcttcaatta accattccaa atctaattca
      241 atcaactttc ctcttcccgg tttaccctct ttatcccatc ccattaattc ataacgtaat
      301 ttttttgcct cttcaaaatc ttctctcttc agtgctccac tgcctttgag aggtccttct
      361 ttaaaatcag taaaaaatat ttcaggcact gtatcatcat ctctagtgaa accctctcta
      421 atgttgaata ttctagccat attaatagtt ctctcaccca atttcatcaa ttcataccag
      481 gtagtattcc aaccagtagt tgcacggatg attgctaaca attcatccat agtacccaaa
      541 ctacggggaa cataaccaaa atcacataca cctaagatgt caaatataga ccaataaata
      601 tccagaattt tatataaggc taccttgcga taacttaaat ctgtcactgc tactggtttt
      661 aagataccca ggccactcat ctcctttaac ccaatagact ccttttcgga aaagaaggca
      721 tcgtgcgggg ctttcatgtg gtctgctcca taatcagaaa aggcatactg cagggcaaga
      781 ccagccttta ctctagggtc atgcatagga acctcctgcc cctttacttc tttcagaaac
      841 cggtcagaac ccttacctac tctttttgaa gccaaacgtg aaccctgtgc taataattca
      901 ccaaatcctt ctctcctggc aatcttttca attaaaacaa gtaaagcctt cttgttgcca
      961 aaatataatt cgagaccatc tgtatctttt ttattcaata aaccctgttc atagcattcc
     1021 atggcaaagg caatgacggc acctgtcgag atggtatcta aaccccattt attgcacaac
     1081 tcgttggctt tagctacata ttgcaaatca tccacaccgc aaagagagcc aaatgcaacc
     1141 agggtttcat actcaggccc accgtaaacc ggatttacag taaactcttc cccattcacc
     1201 tctactatcc ttttacaacg aatggggcag gcatagcaac ttcccttttt tttcaaaatc
     1261 gttttattat actcctctac agttagattt tctccaccct taaaataacc cgttgtccag
     1321 ttaaaagacg gaagtgaacc tgcttcataa aaacctctta ctgtggctgg tgtcccatac
     1381 tctcttaaag ctctacttaa agggttttcc ataactctct gggatatatc tttggctatc
     1441 cccttaactt tatctgaatc atataaagtg atagggatgt tgcccttaac cacaattgct
     1501 ctcagttttt tagaccccat cacagcacct aatccatttc ttccgttaaa atgagtcagt
     1561 tcattaacaa tattggcata tagtacctta ttctctcctg ctataccgat ttgacatatt
     1621 ctgacctttt catcactaaa ttcttctttt attaagcggt ctacctgatg ggtttctttg
     1681 ccccagagat gtgcagcatc tttaaattgc acatccccat ctttaatcca cagatagcag
     1741 ggctttttgg caataccccg gataactatg gc
//
LOCUS       DAOQWT010000370         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_81164_length_1764_cov_3.255705, whole genome shotgun sequence.
ACCESSION   DAOQWT010000370 DAOQWT010000000
VERSION     DAOQWT010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81164_length_1764_cov_3.255705"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKJ95_06950"
     CDS             <1..242
                     /locus_tag="PKJ95_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNR66010.1"
                     /translation="PHPDKPSLGWVNYAELRSGQIDIKGKKVPTSSISSYAKARTIAH
                     TLKEWIEKGEYFLQEPIEFFPSQRELHRLKTTRVY"
     gene            284..691
                     /locus_tag="PKJ95_06955"
     CDS             284..691
                     /locus_tag="PKJ95_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNR66011.1"
                     /translation="MIKKKAILRFDSTTVEKPVTYYLIKKYDLWINILQARFEPSLGG
                     KLVIELRGEKKQLDQAFAYLKEQNIKVDFLEEEISWDKLKCNHCGACVSICPVGALKM
                     NRETYYLEFNYQDCVVCGYCTDACPVQAIEIIF"
     gene            725..1498
                     /locus_tag="PKJ95_06960"
     CDS             725..1498
                     /locus_tag="PKJ95_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HNR66012.1"
                     /translation="MGNKEDRNIYYQSKFDSGHCARSYRDTIKSTDLISFQVKVKESD
                     LLIQAALLLQEEALTSLKYYRTILEDYIKENPIFRTTLNPYPADSNAHPIIREMIEES
                     ARCNVGPMASVAGCIAQYIAIDLLTKTDEIIIENGGDLYIKSHSIRRVIIYAGSSPLS
                     NKLYLKINSSQQGVGVCTSSGTVGPSFSMGEADAVTVLSYSATLADAAATAIGNVVQS
                     KEDISSGLNLAEQIDGILGTIIIKDDKIGMWGEIDYGFV"
     gene            1616..>1764
                     /locus_tag="PKJ95_06965"
     CDS             1616..>1764
                     /locus_tag="PKJ95_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015408017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66013.1"
                     /translation="MFLPTTREEMKKLGWGSLDVIIVTGDTYIDSSFIGASLIGKLLL
                     NAGYRV"
BASE COUNT          588 a          297 c          367 g          512 t
ORIGIN      
        1 gtccacatcc ggacaaacca tctctgggct gggttaatta cgccgagcta cgttccggcc
       61 aaatagatat taaaggcaag aaagtcccca cttcctctat atccagttat gctaaagcaa
      121 gaaccattgc ccatacctta aaagaatgga ttgaaaaagg ggaatacttt ttacaggaac
      181 ctatagagtt tttcccttca caaagggaat tacataggtt aaaaactacc agagtatatt
      241 aataaagaca aataagcaaa ttagtaaaac ttcaggaggg ataatgatta agaaaaaagc
      301 cattttgcgt tttgattcta caacagtaga aaaacctgtt acctattatt taataaaaaa
      361 gtatgatctt tggatcaaca tcttacaggc cagatttgaa ccctccctgg ggggaaagct
      421 ggtaatagag ctgagaggag aaaaaaaaca gttagatcag gcttttgctt atcttaaaga
      481 gcagaatata aaagttgatt tcttagagga ggaaatctcc tgggataaat tgaaatgcaa
      541 ccattgtggt gcctgtgtat cgatatgtcc ggtaggtgcc ttgaagatga atagagagac
      601 atattattta gaattcaact accaggattg tgtggtgtgt ggttattgca ccgatgcctg
      661 tccggtacag gctattgaaa ttatttttta ataatcctgg ttataattat aagaataaat
      721 aggtatggga aataaagaag acagaaatat ttattatcaa tcaaaatttg attctggcca
      781 ttgtgcccgt tcatatcggg atacgattaa aagtaccgat ttaatctcct ttcaggtaaa
      841 agtaaaagaa agtgatcttt taatacaagc tgctctctta cttcaggaag aagccctgac
      901 ttctttaaaa tattaccgga ctatactgga ggattatata aaagaaaacc ccatatttag
      961 aactaccctc aatccttatc cagctgatag caatgctcat cccattataa gggagatgat
     1021 tgaggaatct gcccgttgta atgtgggccc tatggcctct gtggcaggct gtattgccca
     1081 gtatatagca atagacttac taactaaaac agatgaaatt ataattgaaa atggtggaga
     1141 cctgtatatt aaaagccatt caataaggag ggtaattatc tatgccggtt cctccccgct
     1201 cagtaacaag ctgtacctta aaataaactc ctcccaacag ggtgtgggtg tatgtacttc
     1261 ttcaggaact gtgggcccct ctttcagtat gggggaagcc gatgcagtta ctgtcctatc
     1321 ttattctgct accttagcag atgctgctgc taccgctatc ggaaatgtcg ttcaaagcaa
     1381 agaagatata tcaagtggac taaatctagc tgaacagatt gatggcattt taggaacaat
     1441 tataattaaa gatgataaaa tagggatgtg gggcgaaata gattatgggt ttgtttaaaa
     1501 taaggtccat agttttaagt taattgtttt tagtattagg atattcaccg gggataattc
     1561 ttcttactga gaaatatatc aaacagagaa ccgtccccat aaaaaagagg gaaagatgtt
     1621 tttacctact accagagaag aaatgaagaa gttgggttgg ggtagtcttg atgtaattat
     1681 agtgaccggg gatacctata ttgatagttc ctttatagga gcatcattga ttggaaaact
     1741 attactcaat gccggttacc gggt
//
LOCUS       DAOQWT010000371         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_81696_length_1757_cov_3.360165, whole genome shotgun sequence.
ACCESSION   DAOQWT010000371 DAOQWT010000000
VERSION     DAOQWT010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81696_length_1757_cov_3.360165"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /gene="nusA"
                     /locus_tag="PKJ95_06970"
     CDS             <1..490
                     /gene="nusA"
                     /locus_tag="PKJ95_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009169170.1"
                     /note="modifies transcription through interactions with
                     RNA polymerase affecting elongation, readthrough,
                     termination, and antitermination; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HNR66014.1"
                     /translation="RTHPGLLKRLFELEVPEIHEGLVEIKQISREAGKRSKIAVTCKD
                     EMVDSIGSCIGDRGSRIKAIMNELQDEKIDIIKWHEDDKTFIANSLNPAKVLLVELLE
                     KEKRALVVVQDQLLSLSIGKEGQNARLAAKLTGWKIDIKSESQYRQEKKIEQNENISK
                     TT"
     gene            529..825
                     /locus_tag="PKJ95_06975"
     CDS             529..825
                     /locus_tag="PKJ95_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNR66015.1"
                     /translation="MKKKKLPKIPYRTCIGCQGKKAKKDLLRIIRTPDGTVEIDETGK
                     KSGRGAYLCYNKICLQEAIKKKRIGKALKIELSPEIVKELEEKFNTIEENLDDI"
     gene            911..>1757
                     /locus_tag="PKJ95_06980"
     CDS             911..>1757
                     /locus_tag="PKJ95_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HNR66016.1"
                     /translation="MKKRVYEVAEDLKIPAKSLISFLTKEGIDVKNHMSTLDEETIEI
                     IKEAIAEEKKKAKEIEKEEKKVFILDKLPSLKELSEKLNLPLSKVMQQASKLGQIKSI
                     DRELPWEIVDKISQQNNIDISLSGNLKKVIKTKKDRIIEHPLSVKSPVITIVGHVDHG
                     KTTLLDVIRKSNITKKEAGGITQHIGAYVVNLKGNRFVFIDTPGHEAFTTIRARGVKA
                     TDIVVLVVAADDGVMPQTVEAINHARAANVPIIVAINKIDKANANVDKVKKELSKYQL
                     VSEDWG"
BASE COUNT          692 a          243 c          366 g          456 t
ORIGIN      
        1 gcgtacgcat cctggtttac taaaacgatt atttgaatta gaagtccctg aaattcatga
       61 agggttggtg gaaattaaac agatttccag agaagctgga aagagatcca agattgctgt
      121 tacctgtaaa gatgaaatgg ttgattccat cggctcctgt attggagacc gaggctcaag
      181 gattaaagct attatgaatg aacttcagga tgaaaaaata gatatcatta aatggcatga
      241 agatgataag acttttattg ccaattctct taatccagcc aaagtattac tagtggagtt
      301 attggaaaaa gaaaaaaggg ctttggttgt agttcaagat caattgcttt ctctgtctat
      361 tggcaaggaa gggcaaaatg ctcgattggc tgctaagtta acaggttgga agattgatat
      421 taaaagtgaa tcacaataca gacaagaaaa aaaaattgaa cagaacgaaa atataagtaa
      481 aaccacataa aatataagta aattaaaaat ttataccctt tagaaagtat gaagaaaaag
      541 aagttaccca aaataccata tagaacatgt ataggatgtc aggggaaaaa agcgaaaaag
      601 gatttattaa gaattataag aacccctgat ggcacagttg aaatagatga gactggtaag
      661 aaatcaggtc gtggagctta tttgtgttat aataaaatat gtttgcaaga ggcaattaaa
      721 aagaagagaa taggaaaagc attaaagatt gaactttcac cagaaattgt taaagaatta
      781 gaagaaaagt ttaataccat agaggaaaac ctggatgata tttaaaataa ttttacctgt
      841 tttaaaagcg attggctaaa gaacaatatt tccctgataa cggccggttg agatggaggt
      901 cagcatatat atgaaaaaaa gagtttatga agtagcagaa gatttaaaga tcccagctaa
      961 gagcttaatt agttttttaa caaaagaggg tatcgatgtt aaaaatcata tgagcaccct
     1021 tgatgaggag acaattgaaa tcattaagga agcaattgct gaggaaaaaa agaaagctaa
     1081 agaaatagaa aaagaagaaa aaaaagtgtt catattggat aaattgccca gccttaaaga
     1141 gctgtctgaa aagctcaatt taccattatc aaaagtaatg caacaagctt caaaattggg
     1201 acaaataaaa tctattgata gagaattacc atgggaaatt gttgacaaaa taagccaaca
     1261 aaacaatatt gatataagcc tctccggtaa tttaaaaaag gtgattaaaa ctaaaaaaga
     1321 tagaatcatc gaacacccct tgtcggtaaa atcaccggtt attacaattg tgggccatgt
     1381 ggatcatggt aaaaccactc tccttgatgt aatcagaaaa tcaaacatca ctaaaaaaga
     1441 ggcaggaggt attacgcagc acatcggtgc ttatgtggtc aatttgaaag gcaatcgatt
     1501 tgtttttatt gatactccag gacatgaagc atttaccaca atcagggcac gaggggtgaa
     1561 agccactgat attgtagtac tggtagttgc tgctgatgat ggtgtaatgc cccaaactgt
     1621 ggaggcaata aatcatgccc gggcagctaa tgttccaatc attgtggcaa taaacaagat
     1681 tgataaggca aatgctaatg tagataaggt caaaaaggaa ctctctaaat atcagcttgt
     1741 ttcagaagac tggggtg
//
LOCUS       DAOQWT010000372         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_81697_length_1757_cov_3.323737, whole genome shotgun sequence.
ACCESSION   DAOQWT010000372 DAOQWT010000000
VERSION     DAOQWT010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81697_length_1757_cov_3.323737"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..979)
                     /locus_tag="PKJ95_06985"
     CDS             complement(8..979)
                     /locus_tag="PKJ95_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66017.1"
                     /translation="MKIKCPECGYEGEGKFCSQCGAILPTPDESKIDDNEDLKTEIKD
                     TSDLIKEDIPAESVWWTEKCPICKKGKLWHVKQKVFLGLAAVDTLKCMNPDCEATFSE
                     VSDGSYKKYKLTGCKDKLNQSWEKYKNKILLPDEWKAVTYGGISNEEQRENDLKEWLT
                     QLKDGNVKIQLSTTSAPVILKKDEKCYISLPNISLMESRSVRTGGYAGPNIRITKGVS
                     FRVGGFKSSSHEELKTIDDGIFSITNKRIIFSGIKRTININFNKIISMEPFKDGFLVN
                     HEGTKKTQYFTGIPEINISLSAYNRTYHEPLSGLMLVCLIEGTIKQL"
     gene            complement(1055..>1757)
                     /locus_tag="PKJ95_06990"
     CDS             complement(1055..>1757)
                     /locus_tag="PKJ95_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66018.1"
                     /translation="IEKINEVCLEYSEQSLEDNISEKFEGVEIEDLVINKAQRILLVG
                     FSIEEPLSRIIEWLSSNYDLGINAIILNYIKTSSGDELLSKTVIIPEEVEKAKIDKRK
                     YKILMSDEPGNYSDEELKDLLIKYFSKDLYSARRIKNVVLPFLLKNKLASREELKKEF
                     VRVKEAEDTNRAGYFLSLISNQLGQQKKDYLRQIISYEYPNYPWEKDNFKLKGEYREM
                     IESILNILNKNEKVE"
BASE COUNT          520 a          351 c          214 g          672 t
ORIGIN      
        1 tcttttatta taactgtttg attgtgcctt cgattaaaca aactaacatt aagcctgaaa
       61 gtggttcatg ataagttcgg ttataagcag ataaacttat gtttatctca ggtatcccag
      121 taaagtattg tgttttttta gtaccttcat ggttaactaa aaaaccatct ttaaatggct
      181 ccatagaaat aattttgtta aagttaatgt taatagttct tttgatacca gagaatatta
      241 tcctcttatt tgttatagaa aatatcccat catcaattgt ttttaattct tcatgagaag
      301 aagacttaaa tccacctacc cggaaagata cccccttagt aatacgtatg tttggcccag
      361 cataaccgcc tgtacgtaca gatcttgatt ccattaaaga tatgtttggg agcgatatat
      421 agcacttttc gtccttcttt aaaataactg gggcactagt agtgcttaat tgaatcttaa
      481 cattaccatc tttaagctgg gttagccatt cttttaaatc attctctctt tgctcttcat
      541 tgctaatccc tccataagta acagctttcc attcatctgg cagaagaatt ttgtttttat
      601 atttttccca ggactgattc aatttatctt tacatccggt gagtttatat tttttatagc
      661 ttccatcact tacttcacta aaagttgctt cacaatcagg attcatacat ttcaaagtgt
      721 caactgcagc cagccccaga aatacttttt gcttaacatg ccataatttt ccttttttac
      781 agataggaca tttttctgtc caccaaacac tttcagcagg tatatcttcc tttattaaat
      841 cggatgtgtc tttaatttcc gtttttaaat cttcattgtc atcaatcttt gattcatctg
      901 gtgtaggcag aatagcacca cattggctac aaaatttacc ttcaccttca tatccacatt
      961 caggacattt aattttcatt taaatacacc cctcctaact taaaatcaaa ccctttttct
     1021 aaacctttct tataatcaca tatgagacta ttttttattc cactttttca tttttattca
     1081 aaatatttaa aatactttca atcatttccc ggtactctcc cttcagctta aaattgtctt
     1141 tctcccaggg atagttgggg tattcataac taattatctg tcttaaataa tcttttttct
     1201 gctggccaag ctgattagaa atcaatgata gaaaataacc ggctcgattt gtatcctcag
     1261 cttctttaac ccttacaaat tcttttttca gttcttccct tgatgcaagt ttatttttta
     1321 acaaaaaagg caatactaca tttttgatcc ttctagctga atacaggtct tttgaaaaat
     1381 actttataag caaatctttt aattcctcat cggaatagtt gccgggttca tcagacatca
     1441 aaattttata ttttctctta tcaattttag ctttctcgac ttcttcaggg attataactg
     1501 tttttgacaa taattcatca cctgacgaag tttttatata attcaagata atcgcgttga
     1561 ttcccaaatc ataatttgaa gataaccact ctataatacg acttagaggc tcttcaatag
     1621 aaaatcctac taataaaatc ctctgggctt tattaataac caggtcttct atttctaccc
     1681 cttcaaattt ttcagaaata ttatcttcca gtgactgttc gctatattct aaacaaactt
     1741 cattaatctt ttcaatg
//
LOCUS       DAOQWT010000373         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_81784_length_1756_cov_3.376837, whole genome shotgun sequence.
ACCESSION   DAOQWT010000373 DAOQWT010000000
VERSION     DAOQWT010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81784_length_1756_cov_3.376837"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PKJ95_06995"
     CDS             complement(<1..1022)
                     /locus_tag="PKJ95_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66019.1"
                     /translation="MNKFKISSEYCFYTGIFLISFSVLMFELALTRIFSIMQWNYLAF
                     MVISIAFLGYGASGTFLTVVPSLTTKDKQEKLPALLSLFALLYGSSIFLAFFLINGIP
                     FDLYRLKVDSYQWLYLITYYLAVALPFFFAGTCISMAITHQPLKVGNIYFYDLTGASL
                     GCLAFLFFSTRMGIFNLVLMLAILGLAASYLFSLAQEKKLFSLFSLLLIIISLVFFNL
                     PSKRVLTLPINPYKELFTFLRYPETEILYQRENSFSQVKVVHSPAVKHAPGLSLNFSE
                     RIPEQLGILTDDSGLNAITKFDGDIESIRFTDFLTNGLGFHLAQTPDRVLVIGAGGGL
                     DIISAL"
BASE COUNT          613 a          318 c          309 g          516 t
ORIGIN      
        1 tagagagcac ttatgatatc cagcccgcct cctgcaccaa taaccagcac cctgtcaggc
       61 gtttgggcaa gatgaaaccc cagtccgttg gtcaggaagt cggtaaaacg aatagattct
      121 atatctccgt caaatttcgt tattgcattt agccccgaat catcagtaag aatccccagc
      181 tgctcaggta ttctctctga gaaattgaga ctcaaccctg gggcatgctt cactgccgga
      241 ctatgcacta cttttacctg agaaaagcta ttttcacgct ggtatagtat ttctgtttcg
      301 ggataacgaa gaaaagtgaa taattcctta tagggattga tgggcagagt tagtactctc
      361 ttggagggaa gattaaaaaa gactagactt attataatta agagaagtga aaataatgaa
      421 aacagttttt tctcctgagc aagagaaaaa aggtaagagg cagcaaggcc taaaatggcc
      481 aacatcaaaa ccagattaaa tattcccatc ctggtgctga aaaataaaaa agcaaggcat
      541 cccaaagatg ccccggttaa atcgtaaaaa tatatatttc caactttcag gggctgatgg
      601 gtaatagcca tggaaataca ggttccggca aagaagaaag gcaaggcaac tgccagataa
      661 taggtgatta aatagagcca ttgataacta tctaccttta atcggtacag gtcaaagggt
      721 atgccattaa tcaaaaagaa agccaggaag atgctggagc catagaggag tgcgaataag
      781 gacaaaagag ccggaagttt ttcttgttta tcttttgtag tcaaagatgg aacgacggtc
      841 agaaaggtac cgctggcacc atagcctaaa aaagctatac tgataaccat aaaagccaga
      901 taattccatt gcataatgga gaagatcctg gtcagggcaa gttcaaacat caagactgaa
      961 aatgatatta agaatattcc cgtatagaaa cagtattccg atgaaatctt gaatttattc
     1021 atcctttaat accgctgctt ttctttaaaa ttttcctctg atatgctaaa actatattaa
     1081 aactatctta tattgtatta tattcccatc cttttttcat tccccctata ccagaataat
     1141 tgtgagcaac atggttcata gtttacggtt tatagttttc agttaaaagc atcatgtata
     1201 aaatgacaat gaaaattatt tggcacataa cctatgaaca ataaaaatac ataaaaaagt
     1261 tcgagctagt ataaataaat attgtttctc gattagaacg gattgaaggc atgagtaaca
     1321 aaaacttcaa gacataggta acactttttg atactattta tgaattaatg gttaacccat
     1381 aatttaatat tttaccccta cactcttcat ctttttattc tctcatcttt tcctgcaata
     1441 tttctatacg ttatacacta aacggtatat taccttaaca ccaacgacca atttgacgca
     1501 ctgcgcacga cgcacaacga gtgtgtcaga agaaccgtcc ctataaacta taaatttttg
     1561 actaatataa aatgaatgat aaaatctaag taatacattt aatttaattt tttaaactta
     1621 tttttttctt gttatttaac ccaaaaaagg agatgaattt attatgactg attggaagga
     1681 accagctttt agaatagatg aatttaccga cgatatactc gatttttgtg aatctgacca
     1741 cgatttgttt ttagag
//
LOCUS       DAOQWT010000374         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_81967_length_1754_cov_2.439670, whole genome shotgun sequence.
ACCESSION   DAOQWT010000374 DAOQWT010000000
VERSION     DAOQWT010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81967_length_1754_cov_2.439670"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="PKJ95_07000"
     CDS             <1..1531
                     /locus_tag="PKJ95_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNR66020.1"
                     /translation="IAQPALKSPDDIRRLGEPALFWGVTAGSLDSMIANYTATGKKRK
                     RDDFTPGGINNRRPDRATIVYSNLIKQYFKDTRPVVLGGIEASLRRIAHYDFWDNRIR
                     RSILLDSKGDYLIYGMAEKSVLQFAELLSTNSQKITEVRGICYISKTPVQGSIELPTF
                     LEVQKDKKKFTEMFNLFYKNQNPFTAKTLSQKYDNRYLIHNPPSYPLSQKELDKIYEL
                     DYERDVHPYYGKLGRLRALDTIKFSVTSHRGCFGECNFCAITVHQGKCIQSRSQKSII
                     KEIKLLTRRKDFKGYILDVGGPTANMYDMECTKNRRGNYCAEKRCLFPSLCSNLSVSH
                     KAQIELLKAIRKIKGIKKVFVASGIRYDLILADGKNGQHYLSEIIEHHLSGQLKVAPE
                     HIEPEVLSLMGKGNQNQLGRFKKQFDNTNKKKGLKQYLTYYFMSAHPGCSEEDMTQLK
                     NYIKKELKLKPEQVQVFIPAPSTYSALMYYTERNPFTGDKIFVEKKIKNKDKQKKIIT
                     A"
BASE COUNT          577 a          332 c          347 g          498 t
ORIGIN      
        1 cattgcccag ccggctctta aaagccccga tgatatccgc cgcctgggag agccagcact
       61 attctggggt gtgactgccg gttccctgga ttccatgatc gccaattata ctgcaactgg
      121 aaaaaagaga aagagagatg attttacacc cggcggaatc aataatcgca ggcctgaccg
      181 ggcaacaatt gtatacagta acctgataaa acaatatttc aaagataccc gtcccgttgt
      241 tttgggaggt attgaagcca gtctcagaag gatagcacat tatgatttct gggataaccg
      301 aatcaggcgt tccattttat tggatagtaa aggggattat cttatttatg gaatggctga
      361 aaagagtgta ctgcagtttg cagaattgct ctcaaccaat tcacagaaaa ttacagaggt
      421 gaggggtatc tgctatattt ccaaaacacc tgttcagggc agtattgaac tgcccacttt
      481 tcttgaggta caaaaagata agaagaaatt tacggagatg ttcaatctct tttataagaa
      541 ccagaacccc tttaccgcta aaaccctttc tcaaaaatat gataaccgct acctcatcca
      601 caacccccct tcatatccct taagccagaa agaactggat aagatatatg aacttgacta
      661 cgaaagagat gttcatcctt attatggcaa gttagggcgg ttaagggctc tggataccat
      721 caaattttca gttacctcac atagaggatg ttttggtgag tgtaattttt gtgccattac
      781 cgtacatcag ggtaaatgta ttcagtccag aagccagaaa tctataatca aagagataaa
      841 actcctaacc cggcgcaagg attttaaagg atatatcctg gatgttggtg gtcccacagc
      901 caatatgtat gatatggaat gtactaaaaa tagaagaggc aattattgtg ctgagaaacg
      961 ttgtttgttt ccttcactat gttccaatct atcagtcagc cacaaagcac aaatagaact
     1021 gttgaaggca atcagaaaaa taaaggggat taaaaaggta tttgtggcat ccgggatacg
     1081 ttatgatctt atattggcag atgggaaaaa cggtcagcat tatctcagtg agattattga
     1141 acatcatctt tccggacagc taaaggttgc tcctgagcat attgagccag aagtattaag
     1201 tttaatgggt aagggtaatc agaatcagct gggaagattc aaaaaacaat ttgataatac
     1261 caataagaag aaaggcctga aacaatacct tacctattat tttatgtctg cacaccctgg
     1321 ctgcagtgag gaggatatga ctcaactaaa gaactatatt aaaaaagaac taaaattaaa
     1381 acctgaacag gtacaagtct tcattccagc accatccacc tattcagccc tcatgtacta
     1441 cacagaacgt aaccccttta ccggtgataa aatctttgta gaaaagaaga ttaaaaacaa
     1501 agataaacag aaaaaaatta tcaccgcttg atagtttatt aacaattttt ctattattga
     1561 acttacttgt tattagtgcc tggtctatat tcgtaataat tccaatagtg cctgcttagc
     1621 tttatctttg ggataaagtg atagcttttt agtggttttc ttatccagat cttgaagcat
     1681 tttggggata ggaagttttg tatattcact caattctcgc aacaggacaa attcttcttt
     1741 gttgtgaaca gaat
//
LOCUS       DAOQWT010000375         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_82004_length_1753_cov_3.410483, whole genome shotgun sequence.
ACCESSION   DAOQWT010000375 DAOQWT010000000
VERSION     DAOQWT010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82004_length_1753_cov_3.410483"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..433
                     /locus_tag="PKJ95_07005"
     tRNA            339..433
                     /locus_tag="PKJ95_07005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:373..375,aa:Ser,seq:gct)
     gene            478..987
                     /locus_tag="PKJ95_07010"
     CDS             478..987
                     /locus_tag="PKJ95_07010"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR66021.1"
                     /translation="MPHQHSLKDVIIRNDFQYGDLGIVLSMHGELYHKEYQLNHEFEA
                     YVAEGIVEFARQYQEGRSRLWIAEDKGKAIGTVTIMERSPQQVQFRWFLIHPDYRGIG
                     LGKILVEKTIAFSQEAGYHSIYLWTLEHLTAARILYKRFGFKLDDKKTHFLWGQNLTE
                     ERYLLNLKK"
     gene            1028..1426
                     /locus_tag="PKJ95_07015"
     CDS             1028..1426
                     /locus_tag="PKJ95_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNR66022.1"
                     /translation="MAKKLYRSKKDSIIGGVCGGIAEYFEIDPTLIRLLAILVVFLGG
                     VGVIAYIIAWIIIPLNPEELPVQEKDYSSSDGHEGRNKNIWGGVILIMLGLFFLAKSF
                     FPHFVLVKFWPVILVIVGIGLIIQSLTRNK"
BASE COUNT          568 a          266 c          325 g          594 t
ORIGIN      
        1 acaaacaaat tttcatcctc aaaaaatttc catcgttcca ataaaaaaag ataacctatg
       61 ttaattaatt catatatttt ttcttttaat tctaaggatt aaagtttttt aattactatt
      121 ataaatgtct ttcctttttt tgtcattgcc aactaaatgt tttaatatta aaagtgtctt
      181 ttcataaaaa tattaacaac attcttaaaa aataaataac tataacattt gatagtttca
      241 gtaaaaaatg taatatttgg ttagttaaca tggtcagttt tttaatctga ctctattttt
      301 ttgttgcctc aaatgtgcta ttatgctata ctttaatcgg agagatgacc gagaggccga
      361 aggtggttgc ctgctaagca attgagcgga ttttaagtcc gctccgaggg ttcgaatccc
      421 tctctctccg ccattttttt atttttacta tttttaaaaa aggatcaaat tgaaatcatg
      481 cctcatcagc attctttgaa ggatgttatt attaggaatg attttcagta tggagacctg
      541 gggatagtgc tatcaatgca tggtgaactg taccataaag aataccagct gaaccatgag
      601 tttgaagcat atgtagccga aggaattgtg gagtttgcca gacaatatca ggaaggaaga
      661 agcaggctgt ggattgccga ggataagggg aaagcaatcg gtacggtgac tataatggaa
      721 aggtcacccc agcaagttca atttcgctgg tttttaattc atcctgacta tcgaggaata
      781 gggctgggaa aaatattggt ggaaaaaact atcgctttta gccaggaagc tggctaccat
      841 agtatttatc tttggacctt agagcaccta actgcagcca ggattcttta taagagattc
      901 ggatttaaac tggatgacaa aaaaacacac tttttatggg gtcaaaacct aacagaagaa
      961 cgttaccttt tgaatcttaa aaaataaata gtataaacgc tttttataaa tcaggaggaa
     1021 cctgttaatg gcgaaaaaac tttatcgttc taaaaaagat agcattattg gtggagtttg
     1081 cggaggaatt gccgaatatt ttgagattga cccaacctta atccgacttt tagcaatact
     1141 ggtagttttt cttggcggag tgggggtcat cgcttatata attgcctgga ttattattcc
     1201 cctgaatccc gaagagttgc cggtacaaga gaaggattat tccagctcag atggtcatga
     1261 gggcagaaat aaaaatatct ggggaggagt aatacttatt atgttaggac tattcttttt
     1321 agctaaaagt ttctttcccc attttgttct ggtcaaattc tggcctgtta ttctggttat
     1381 agtgggtatt gggttaataa ttcaatctct tacccgaaac aaataacctt agatatcttt
     1441 tttaattaat aatctctccc ttttccccac tttaattatc aattcatata agcagaatgt
     1501 ataataatat aagaattcct aaaatagtga aacgtgttct atcattgttt tatacagatt
     1561 gaattcggct acaggggaag gcgaatgtag tagttagata acctggtaat tttttcttac
     1621 ttttcatagg gtgagatatt ctatatccca tgttctagct actaagataa ttgtgaacat
     1681 agggtttata gttcatagtt tatagttaat aataaaattc aaagtagtta taggtaaaat
     1741 agaaagtata aaa
//
LOCUS       DAOQWT010000376         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_82472_length_1747_cov_2.596336, whole genome shotgun sequence.
ACCESSION   DAOQWT010000376 DAOQWT010000000
VERSION     DAOQWT010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82472_length_1747_cov_2.596336"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..924)
                     /locus_tag="PKJ95_07020"
     CDS             complement(85..924)
                     /locus_tag="PKJ95_07020"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNR66023.1"
                     /translation="MAFLLILDREHKVLWVKSKNRISDDGWMLPEVEDPVNKPSNRLN
                     VELLSKQAFIPLKSSLIGKKLIFDIFRNEQQLYLFLCPGKESSEILFDLTKYKNIQWN
                     PINCIEEEECFSTLTGRSIIEAYYDILLPEFDDRKNRFHIVKNGFGKKDIDEHNQMIL
                     QGSSEFFVELVEQYNLYRQPLPKIGDHTFLNNWKGLPVAVLEITGVALEPFEKIDGYI
                     KDSENQKRDGINKTVEQYRKYFQKWSKKYNKQFSDSKSVLISQFKIIRKLNEKKMDYL
                     DLT"
     gene            1239..>1747
                     /locus_tag="PKJ95_07025"
     CDS             1239..>1747
                     /locus_tag="PKJ95_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNR66024.1"
                     /translation="MPKRKNRKLTEGNIIKNLLVVSLPTMFGFFAQTLYDFIDMIWIG
                     RISTQAVAGVAIFVTIFWFADIVNEIIGISAVSLISQNYGAGRLEKTREIIEQSIFFK
                     GIVSFCMAVFLLLILRPLSNFFSNESSVIKHILDYGIIRTVFLPFMFLSFSVNTALRC
                     IGDARSPMY"
BASE COUNT          548 a          284 c          282 g          633 t
ORIGIN      
        1 ggactgtgtg ggaaccttat tccagtcaac aaggacactc ccttaaatat aaccattgaa
       61 gatttaataa ttaaaattta atagtcaggt taaatctaag taatccatct ttttctcgtt
      121 aagtttcctg attattttaa attgtgagat aagtactgat ttgctatcag aaaattgttt
      181 attgtatttc ttagaccatt tttgaaaata ttttctatat tgttcaactg ttttattaat
      241 tccatctctt ttctggtttt cactatcttt tatataacca tcaatctttt caaatggttc
      301 cagtgccact ccggtaatct ctaagacagc taccggtagt cccttccaat tatttaagaa
      361 agtatggtca ccaatctttg gaagaggttg acgatataag ttatactgct ctaccagctc
      421 aacaaagaat tcagaactgc cctgcagtat catttgattg tgctcatcga tatctttctt
      481 cccaaatcca tttttaacaa tatgaaagcg gttttttcta tcatcaaatt caggtagcag
      541 gatatcataa taagcttcaa taattgatct tccggtcaga gtagaaaagc attcttcctc
      601 ttcaatacag ttaattgggt tccactgaat atttttgtat ttggttaaat caaaaaggat
      661 ttcagatgat tctttcccgg gacaaagaaa aagataaagt tgttgttcat tacggaagat
      721 atcaaaaatt aattttttac caattaaact cgacttaagt gggataaaag cctgctttga
      781 caggagttca acattcaatc gattagaagg tttgtttaca gggtcttcaa cttccggtaa
      841 catccagcca tcatcggaaa tccgattttt gcttttgacc cataaaacct tatgctctct
      901 atccaaaatt aagagaaaag ccataacaac tcctgcaggt attgttgtta aggaaagaat
      961 tgtttgtttt cgtcaatatc aaattttcag agggttatac atggtgcaag attgctgctg
     1021 ttacttttag gatgctcttc aggaattata acattttaac tttatctatt gtaaatcaat
     1081 ttcttggaaa aaaatcaatt ttataatatt atagattgga aaagactttt aaagtctttt
     1141 tttgttatta tagcacagca atctaaaata attaatataa ttcatcaaat tgaaataaat
     1201 tttttgtaga attagaaaaa tatataaagt aattatttat gcctaaaaga aagaatagaa
     1261 aattaactga aggaaacata atcaaaaatt tgctggtggt ttctctaccc accatgtttg
     1321 gattttttgc ccagactctt tatgacttta tagacatgat ctggattgga agaatttcta
     1381 ctcaggcagt agcaggggtg gctatttttg taaccatttt ctggtttgct gatattgtaa
     1441 atgagattat cggcattagt gcagtatcac ttatctctca gaactatgga gcaggcaggc
     1501 tggagaaaac aagagagata attgagcagt caattttctt taaagggatt gtttcttttt
     1561 gtatggcagt ttttttgctc ctaattttaa gaccactcag taattttttt tcaaatgaat
     1621 catcggttat aaaacatatt ctggattatg gaattatccg tacagttttt cttcctttta
     1681 tgtttctctc cttcagtgtc aatactgccc ttcggtgtat aggagacgcc aggagcccta
     1741 tgtacat
//
LOCUS       DAOQWT010000377         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_82524_length_1746_cov_4.469545, whole genome shotgun sequence.
ACCESSION   DAOQWT010000377 DAOQWT010000000
VERSION     DAOQWT010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82524_length_1746_cov_4.469545"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..540)
                     /locus_tag="PKJ95_07030"
     CDS             complement(43..540)
                     /locus_tag="PKJ95_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66025.1"
                     /translation="MEKQDKPEYKFYRYNLNEKNIDAFIEKLKGIFADMPLLEKGNIK
                     VKYPLTAWFTCNIPEGRKIFLENIRNSLLWYEIKHNCTFNPEKYYGKDYDLLLNSNEY
                     KLKKDNIFHQKLRKKTIVKDVLECHFTEKLISSDFPKRLFNYLCKEREVECLTVYLPP
                     KLLNK"
     gene            complement(660..>1746)
                     /locus_tag="PKJ95_07035"
     CDS             complement(660..>1746)
                     /locus_tag="PKJ95_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010503696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR66026.1"
                     /translation="PGISYEQMANVAQWRMEEDAMKTAQTALVNFHHQLDLPLLWGDG
                     TTSSSDGMRVELGVSALNAQANPHYGTGKGTTFYRFTSDQYSSFYTTIINTNARDAIH
                     VIDGLLHHETDLEIKEHYTDTLGYTDQVFGLTHLLGFHFAPRLRDLSESKLYFWDKAN
                     DFPKIKRYFKGKINSKIIRENYDDVLRLADSILKGKVSGSLIMGKLGSFAKQNKIAKA
                     LKEMGNIEKTIFILDYIMDEDFRRRVQRGLNKGEAINSLARALFFGKLGVLRELSIKN
                     QFQRAAALNILINAISIWNTVYLNKAITELKQRKDLKNLNEDLFKHISPLGWRHINFL
                     GDYTFDFTRKTTLDSLRPLKTKGGYDF"
BASE COUNT          550 a          313 c          254 g          629 t
ORIGIN      
        1 tataatagcg tcaattttca tttacaacgg gggggtgatt ttttacttat ttaacaattt
       61 aggaggtaaa tacactgtaa gacattccac ttctctttct ttgcataaat agttaaatag
      121 tcttttggga aaatcagagc ttattaattt ttctgtaaaa tgacactcta aaacgtcttt
      181 tactatagtt tttttcctaa gtttctgatg aaagatatta tctttcttta gcttatattc
      241 attggaattt aataataaat cataatcttt accatagtat ttctcaggat taaaagtaca
      301 attatgtttt atctcatacc ataaaagaga atttctgata ttttcaagaa aaatctttct
      361 tccttcaggg atattacagg taaaccaggc agtaagagga tactttactt ttatatttcc
      421 tttttctaac aatggcatat cagcgaaaat acctttaagc ttttctataa aagcatctat
      481 atttttttca ttaagattat atctataaaa tttatattct ggtttatctt gtttttccat
      541 aacttttcct ttctaaaaat ttccactcct acatggggta ccgggatcaa aacgacgaat
      601 tttggcgtta aggtgatttc ctcagtcata ccaacggttt ttactatcta aaaattaagt
      661 taaaaatcat atcccccttt tgttttcaac ggtcttaaag aatccagtgt agttttcctg
      721 gtaaaatcaa aggtataatc tccaagaaaa ttaatatgcc tccagcccag aggagatata
      781 tgtttaaaaa ggtcttcatt taaatttttc aggtcttttc tctgttttaa ttcagtaata
      841 gccttattaa gataaactgt attccaaata ctaatggcat ttatcagtat atttagagca
      901 gctgctctct gaaattgatt tttaatagat aactccctta atactcccag tttaccaaag
      961 aaaagagctc ttgcaagaga attaatagcc tctcctttat ttaagcccct ctgtaccctt
     1021 ctacggaaat cttcatccat aatataatct aaaataaaga tagtcttttc gatattgccc
     1081 atctctttta aagctttggc aattttattt tgtttggcaa aagagcctaa ttttcccata
     1141 ataagagagc cagaaacttt tcccttaaga attgagtcgg ccaatctcaa aacatcatca
     1201 taattttccc tgataatttt gctatttatt tttcctttaa aatatctctt tattttcgga
     1261 aaatcattag ctttatccca aaaataaagt tttgattccg acaggtctcg taatctggga
     1321 gcgaaatgaa agccaaggag gtgagtcaat ccaaatacct gatctgtata gcccagagta
     1381 tcagtataat gttctttaat ttccaggtct gtttcatgat gtaaaagacc gtcaataaca
     1441 tgtatggcat ctctggcatt ggtgttaatt atcgtagtat aaaaagaaga atactggtca
     1501 cttgtaaaac gataaaaagt tgttcctttc cctgtgccat aatgagggtt tgcctgtgca
     1561 tttaaggctg aaacgcctaa ttccactctc ataccatctg aagaagatgt agttccgtca
     1621 ccccataata aaggcaggtc aagttgatga tggaaattaa caagagcagt ctgtgcggtt
     1681 ttcatagcat cttcctccat tctccactga gctacatttg ccatctgttc ataagaaata
     1741 cctgga
//
LOCUS       DAOQWT010000378         1741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_82949_length_1741_cov_2.945433, whole genome shotgun sequence.
ACCESSION   DAOQWT010000378 DAOQWT010000000
VERSION     DAOQWT010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82949_length_1741_cov_2.945433"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="PKJ95_07040"
     CDS             <1..1546
                     /locus_tag="PKJ95_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNR66027.1"
                     /translation="GDMKLEKRLGDKLRIIKKGNIDNLPPIFSFSRLVKIGKEALLKG
                     DQHFLNISQHALPDDLITIIYTSGTTSDPKGVMLTHRNIMHNVVNVPLTVGDFNENDR
                     FLSVLPSWHIYERTVEYCAMGMGASTAYSKPFKQILLPDLLLEKPTIMCTVPRIWHSL
                     YKGILQKIKMGAKLQQLLFFKGLDIAKKYKHAKRMLEDTLPLFDRAQFSEQEKGNARK
                     TIRNLSLLYKMSDKLVFKKIRDMLGGEIKFAISGGGSLPEDIDIFLDAVGVLVLEGYG
                     LTETSPVVAGRKQLNPIIFTVGEPIIDTFIKIRDKEAIDLEVSDGHPGIVFVKGDLVM
                     KGYYKNEQKTAEVLKDGWFNTGDLGKKTYNGKYLKIIGRIKDTIVLAGGENVEPQPLE
                     EKLKESPYISMVIVVGQDKSRLGALIVPDFEALKEYAHKKHLDYQDNAELIKKKEVLS
                     LYQQEQKRLISKEYGFQPFETVMGIALLADEFSQEKGEMTESLKLKRFIIHEKYQKEI
                     DRICAK"
BASE COUNT          592 a          279 c          354 g          516 t
ORIGIN      
        1 tggagatatg aaattagaaa aaagattagg ggataagctc cgtataatta agaagggtaa
       61 tattgacaat ttgcctccta ttttttcttt ctctcggctt gtaaaaattg gtaaagaagc
      121 tttactgaaa ggtgaccagc actttttaaa tatttcccaa catgcacttc ctgatgattt
      181 aataactata atttatacct ctggcactac ctctgacccc aagggtgtta tgttaaccca
      241 tcgcaatatt atgcacaatg ttgttaatgt acctcttacc gtgggagatt tcaatgaaaa
      301 tgaccgtttt ctttctgtac tgccatcatg gcatatttat gaaagaacgg ttgaatactg
      361 cgctatggga atgggtgctt ccactgccta cagcaaacca tttaaacaaa tattattacc
      421 ggacctcctc ctggaaaaac cgaccataat gtgtaccgtt ccccgtatat ggcacagcct
      481 ttataaggga attctgcaaa agataaaaat gggagcgaag ttgcagcaat tactattttt
      541 caagggattg gatattgcta aaaaatacaa acatgctaaa agaatgcttg aggatacctt
      601 gcccctcttt gatagggctc aattttccga acaagaaaag ggaaatgcca gaaaaactat
      661 cagaaatcta tctttattat ataaaatgag tgataagttg gtctttaaaa aaattcggga
      721 tatgttaggt ggagaaatta aatttgctat aagcggcggg ggatctctcc ctgaagatat
      781 tgatattttt cttgatgcgg taggcgtact ggttttagag ggatatggtt tgacagaaac
      841 ctctccggta gttgcaggaa gaaaacagct taatcccatc atattcacag taggcgagcc
      901 aattattgat acttttataa aaattaggga taaggaggct attgacctgg aggtttccga
      961 tggccaccca ggtatagttt ttgttaaagg cgatctggtt atgaaaggtt attacaaaaa
     1021 tgaacagaaa acagcagaag tgctcaagga tggttggttt aataccggcg atctggggaa
     1081 aaaaacttat aatgggaaat atctgaaaat tataggtaga ataaaggata ccatcgttct
     1141 ggcgggagga gaaaatgttg aaccacagcc gctggaagaa aaactcaaag agagccctta
     1201 tattagcatg gtaattgtgg tggggcagga taaatccaga ctgggtgctc taatcgtccc
     1261 tgattttgag gctctcaaag agtatgccca taaaaaacat cttgattatc aggataatgc
     1321 agagcttatt aaaaagaaag aagtgttatc cctttatcaa caagaacaaa aacgattaat
     1381 atccaaagaa tatggttttc aaccctttga gacagttatg ggtatagctt tactggctga
     1441 tgaattctct caggagaaag gagaaatgac agaaagtctt aaattaaaaa gatttattat
     1501 tcatgaaaaa taccagaaag aaattgatcg gatatgcgca aaataaaata tggcactact
     1561 gactatatca atactacttt tttataattt atgctgattt taaaatatct atacacctta
     1621 gtgaaagagt gaagattggt actttttttt ggtgcactga ttattcaggt aatataaact
     1681 gctgataggg ataaattaaa tcaggatttt gaatcaattc ctcattagcc ctgaatatct
     1741 t
//
LOCUS       DAOQWT010000379         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_83018_length_1740_cov_3.839169, whole genome shotgun sequence.
ACCESSION   DAOQWT010000379 DAOQWT010000000
VERSION     DAOQWT010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83018_length_1740_cov_3.839169"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1662
                     /locus_tag="PKJ95_07045"
     CDS             <1..1662
                     /locus_tag="PKJ95_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNR66028.1"
                     /translation="ALLCNNSYLRQSESTKQWEIVGDPTEGALVVVAAKAGYQKEELE
                     NIYPRLRELLFDSERKRMSTIHRTPEGKEILFAKGAPEQVLERCSNYRNKSEILPLSL
                     SIKEKITEQNNRLALEALRVLAVAYREINQQEKENIVGKKKLDIDKTESELTFLGLIA
                     MIDSPRKEAMEAVQTCRRAGIRPVMITGDYALTAKVVAQQLDIFRPGDNLITGSELEK
                     MSQDELEKVVMKTTIFARVSPQHKMKIVRAFQKNNQVVAMTGDGVNDAPAIKKSDIGI
                     AMGITGTDVAKEAADMVLTDDNFSSIVSAVEEGRKIYQNIQKFLNYLLSCNLGEILTI
                     FVAILIGLPRPLLPIQILWVNLVTDGLPALALGLDPAEKDVMRRPPRNPEEGLFSGKV
                     GFNIFTQGFFIGTITLIAFYYGYSQYSLEVGETMAFGTLSFSQLWHSLNSRSVKFSIF
                     KLGIFSNHYLILAILASGFLQLSVMIIPFLQSVFQISSLTLYQWMMIILISLTPILYV
                     EIQKKLKLTYLKYPVRFKRTVLTKFFLTFKRETLLIKLIITFRGR"
BASE COUNT          553 a          301 c          375 g          511 t
ORIGIN      
        1 gctctcctct gtaacaattc ttatttgagg caaagcgaaa gcaccaaaca atgggagatt
       61 gtaggtgacc caactgaagg agctttagtg gtggttgccg ctaaagccgg ctatcagaaa
      121 gaggaattag aaaacatata tcctcgttta agagaactac tctttgattc agaacgaaaa
      181 agaatgtcta ccattcatag aactcctgag gggaaagaaa tattatttgc caaaggggct
      241 ccagaacagg tcctggaacg ttgtagcaat tatcgaaata aaagtgaaat attacctctg
      301 tctctatcta taaaagagaa aattactgaa cagaacaaca gattggcctt agaggcactt
      361 cgggtgttag ctgttgctta tcgtgaaata aatcagcagg aaaaagaaaa tatagtaggg
      421 aaaaagaaat tagatataga taaaacagag agtgaattaa cttttttagg tttaattgcc
      481 atgattgatt ctcccagaaa agaggcaatg gaagcagtgc agacctgccg cagggcggga
      541 attcgtccgg ttatgataac aggagattat gcccttacgg cgaaagttgt tgcccagcaa
      601 ctcgatattt ttaggccggg agataactta ataaccggtt cagaattaga gaaaatgtca
      661 caagatgagc tggaaaaagt ggtaatgaag acaaccatct ttgcccgggt atcaccacag
      721 cataaaatga aaattgtccg ggcttttcag aaaaataacc aggtggttgc tatgaccggc
      781 gatggtgtaa atgatgcacc tgccattaaa aaatcggata ttggaatagc tatgggcatt
      841 accgggaccg atgtagccaa agaggcggca gacatggttt tgactgatga caatttttct
      901 tctattgttt cggcagtaga agagggaaga aaaatatatc agaacattca aaagttttta
      961 aattatctat tatcctgtaa tttaggggag atccttacta tttttgttgc tattctgata
     1021 ggtctgcccc gtcctctgct ccccattcaa attctctggg tcaatctggt tactgatggc
     1081 ctacctgccc tggcactggg cctggacccg gcggagaagg atgtgatgag gaggcctcct
     1141 agaaatcctg aggaagggct tttttcgggg aaggtggggt ttaatatctt cacacagggg
     1201 ttttttattg gaactataac tctcatcgcc ttctattatg gttatagtca gtattctctg
     1261 gaggtgggag aaacaatggc ttttggcact ctcagtttta gtcagctatg gcattcccta
     1321 aattctcgtt ctgttaaatt ttcaatattc aagttaggaa ttttcagcaa ccattattta
     1381 atactggcaa tcctggcctc tggcttttta cagctgtctg ttatgataat acctttttta
     1441 cagtcggttt ttcagattag ctcactcact ttatatcaat ggatgatgat tatacttatc
     1501 tctctgacgc cgatactcta tgtggagata caaaaaaagc ttaaattaac ttatttgaaa
     1561 taccctgtca gatttaagag aacagtttta acaaaatttt ttctgacttt taaaagagaa
     1621 acactattaa taaaattgat aatcactttc agaggaagat agcacaaaaa agtatgaaaa
     1681 tctaagtctg ccattattta tagtatgaat atggtatact atagaattag atttagagga
//
LOCUS       DAOQWT010000380         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_83343_length_1736_cov_3.414039, whole genome shotgun sequence.
ACCESSION   DAOQWT010000380 DAOQWT010000000
VERSION     DAOQWT010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83343_length_1736_cov_3.414039"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1736)
                     /locus_tag="PKJ95_07050"
     CDS             complement(<1..>1736)
                     /locus_tag="PKJ95_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66029.1"
                     /translation="NNVANAQTDFLFDKQIEANAITRFWEGFTEYEIKLFWSPFQLEM
                     ADEYRIFKSINGGDFEQVKADYKDEGFQLSWTDEDVLDKNSYSYYIEGYFFGELVART
                     EQVDVDFWLPSCPALFPVNNEIVSEEEPEFRWQTIVITAFPFKNVIFSAEGEFVVYDL
                     TEEEEIWRIPLEDINSSQLNFKIDEADKPLQKKHRYQWQLKITGYGSGNQVIAESITG
                     GLFVFQEAAEEIVPEEEEEYVEGRLSIDAGMVSYQVIDDEDVIVAQENVKLIYKDISL
                     QANYLQVILDRNELIAKEKVLFNIGEESYSCQHLNYNWKTDRFTMEGFSGETSGENVR
                     GLVYYKGGKMENFPDTIEISSGFFTTCDLEEPHWHIEAEQIIIYIDDKIVAKKVSWYE
                     GKKKMFTLPSFLIFLRGKNQLPYIPDIGRNSSEGWFLKNRINYVKDASSYGSVYIDLM
                     EQKGLAAGIEHTFELGEERVDDGEVVLYLYALKRKAVNIYDVDANINYWQNFENDLRL
                     KANVSYSSSINPDSLTGTNHSIKPDFYIYKKWEDSLLTVTGKYNFNVKESNLNSSGNL
                     KMVYDHTIADDL"
BASE COUNT          503 a          387 c          267 g          579 t
ORIGIN      
        1 taggtcatct gcaatagtat ggtcatagac catcttgaga ttaccactac tgtttaaatt
       61 gctttctttt acattaaaat tatattttcc agttacagtt agcagtgaat cttcccattt
      121 tttataaata taaaagtcag gctttataga atgatttgtg cctgttaaag agtcagggtt
      181 aatactgctg ctataggata catttgcttt caaccttaaa tcattttcga aattttgcca
      241 gtaattgata ttggcatcta catcgtagat attgaccgct tttcttttta aggcataaag
      301 ataaagaacc acttcaccat catccactct ctcttcgccc aattcaaagg tgtgctcaat
      361 tccggctgcc agaccttttt gttccatcag atcaatataa acactgccat aagaggaggc
      421 atctttaaca taattaattc tattttttaa aaaccatccc tcactactgt tccgtcctat
      481 atccggtata tagggaagct ggttttttcc tcttagaaag attaaaaaag agggaagggt
      541 aaacatcttc tttttccctt cataccagga aaccttctta gcaactattt tatcatcgat
      601 atagataata atctgttctg cctcgatatg ccagtgcggt tcttccaggt cacaggtggt
      661 aaaaaaccca cttgatattt caatagtatc tggaaaattt tccatttttc cacccttata
      721 atagaccaac cctctcacat tttctcctga agtttcaccg gagaaacctt ccatagtgaa
      781 tctgtcagtt ttccaattat aatttaagtg ctggcaggaa tagctttcct ccccaatatt
      841 aaataatact ttttctttgg cgattaactc atttcggtct aaaatcacct gaagataatt
      901 tgcttgaaga ctgatatctt tatagataag cttgacattt tcttgagcga caatcacatc
      961 ctcatcatca ataacctggt aactaaccat accggcatca atgctcaatc ttccctctac
     1021 atattcttct tcctcttcag gcacaatttc ttcggcagcc tcttgaaata caaaaagtcc
     1081 gccggtgata gattcagcaa taacctgatt gcctgaccca taaccggtaa ttttcaattg
     1141 ccactgatag cggtgttttt tctgcaaagg tttatctgcc tcatctatct tgaagttaag
     1201 ctggctggag tttatgtctt ctaaggggat tcgccatatc tcttcctcct cagttaaatc
     1261 atagactaca aattctcctt cggcagagaa aataacattt ttaaaaggaa atgcggttat
     1321 tacaattgtt tgccatcgga attcaggctc ttcttctgac acaatttcat tgttaacagg
     1381 gaatagggca gggcaggagg gtagccagaa atcaacatcc acctgttcgg ttcttgccac
     1441 cagctccccg aaaaaatacc cctctatata ataactataa ctgtttttgt ccagcacatc
     1501 ctcatctgtc caggaaagct gaaatccctc atctttataa tctgctttta cctgttcaaa
     1561 gtcaccccca ttgatacttt tgaaaattct gtattcatca gccatttcca gctgaaaggg
     1621 ggaccagaac aatttaatct catattcagt aaatccttcc cagaacctgg taatggcatt
     1681 ggcttcaatc tgtttgtcaa ataaaaaatc tgtttgagca ttagctacat tgttca
//
LOCUS       DAOQWT010000381         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_83662_length_1732_cov_4.720930, whole genome shotgun sequence.
ACCESSION   DAOQWT010000381 DAOQWT010000000
VERSION     DAOQWT010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83662_length_1732_cov_4.720930"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /gene="dapF"
                     /locus_tag="PKJ95_07055"
     CDS             complement(<1..489)
                     /gene="dapF"
                     /locus_tag="PKJ95_07055"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNR66030.1"
                     /translation="MELDFTKMHGTGNDFIIMDFYSKTFPDGLDFHNTAKKICARHFG
                     IGADGLILILPSDKYDFKMRIFNANGSEAQMCGNGIRCLAKYAYENKLTHKDVFSIET
                     LAGIIIPELIFEEQKTGVIKGVRVDMGKPHLTKKNIPMIGNPDRQVINEIISLGNGQY
                     FRI"
     gene            complement(523..>1732)
                     /gene="lysA"
                     /locus_tag="PKJ95_07060"
     CDS             complement(523..>1732)
                     /gene="lysA"
                     /locus_tag="PKJ95_07060"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNR66031.1"
                     /translation="REQCQEHIKAFDKCNIDYEVLYAGKAFLVQAMAHILNEESMSLD
                     VSSAGEIYMALEGGFPAERIYFHGNNKSKEELNFALERNVGTIIIDNFCELESLEQIA
                     NKLNKKVNIMVRIIPGVDTHTNEKIKTGQVDSKFGIPIRDFLDFFPEIITKNHLIYQG
                     IHCHIGSQLFDMQSYHLAAKEMIETIKSIEDKYRVKTNMLNLGGGLGARYTQKDPYLS
                     ISDYVHQLVKKVKEICSKKQLAVPKLLVEPGRSIIAEAGITLYSIGAIKEIKGLKKYL
                     IVDGGMADNPRPSLYDARYEALIVNKFGEEAVENVTVAGKYCESGDVLIDNIKLPQAE
                     CGDLLVFFTTGAYHYAMSSNYNGFPRPPVILVNKGMDGLMVKGETYQYLNQNHIIPEW
                     LNHNGTHGAK"
BASE COUNT          516 a          368 c          249 g          599 t
ORIGIN      
        1 tattctaaag tattggccat tccccaaaga aataatttca ttaataacct gtcggtcagg
       61 attaccaatc ataggaatat tttttttagt taaatgaggt tttcccatat caactctaac
      121 acctttgatt acccctgttt tttgttcctc aaagataagt tcagggataa taataccagc
      181 cagggtctca atggaaaata catctttatg ggttaattta ttttcgtagg catattttgc
      241 caaacaacgg attccatttc cacacatctg ggcttcactg ccgttggcat tgaatatcct
      301 catcttaaaa tcatacttat ctgaagggag aatcaggatt aatccgtctg ctcctatgcc
      361 gaaatgacga gcacaaattt ttttagccgt attatgaaaa tctaaaccat caggaaatgt
      421 tttagaataa aaatccatta taataaaatc atttccagtt ccatgcattt tggtaaaatc
      481 aagttccata ccccactcct cctgatattt ttttatctca ttttatttag caccatgagt
      541 cccgttatga tttaaccatt ccggaataat atgattttga tttaaatatt gatatgtttc
      601 tcctttcacc ataaggccat ccatcccttt atttactaaa atcaccgggg gcctgggaaa
      661 accgttataa ttgctggaca tagcataatg gtatgctcct gtagtaaaga aaactaataa
      721 atcaccgcac tctgcttggg gtaattttat attatcaatt agaacatcgc cagactcaca
      781 gtatttacca gcaactgtaa cattttccac cgcttcctca ccaaatttat taacaatcag
      841 agcctcatat ctggcatcat agagactggg gcgcggatta tcagccatac cgccatcaac
      901 tattaaatat ttttttaaac cttttatttc ctttattgct ccgatactgt atagagttat
      961 tcctgcctca gcaatgattg atctcccagg ttcaacaaga agcttaggaa cagccagttg
     1021 ctttttacta caaatttcct ttactttttt tactaactgg tgcacataat cactgatgct
     1081 caggtatggg tctttctggg tatatctcgc ccccaatcct ccgcccagat tgagcatatt
     1141 tgtctttact ctatatttat cttcaattga tttaattgtt tcaatcatct ctttggcagc
     1201 taaatggtaa gattgcatat caaagagctg tgaaccaata tgacaatgaa tcccctggta
     1261 tatcaagtgg ttttttgtaa ttatttccgg aaaaaaatct agaaaatctc gaataggtat
     1321 tccaaatttt gaatcaacct gaccagtttt tattttttca ttggtatgtg tatcaactcc
     1381 tggaataatt ctgaccataa tattaacttt tttatttaat ttattggcta tctgttctaa
     1441 tgattccaac tcacaaaaat tatcaattat aatcgtgccc acatttcgct caagggcaaa
     1501 attcaattcc tcttttgatt tattatttcc atggaaataa atcctctcgg cagggaaacc
     1561 accttctaat gccatataga tttctcctgc agaggagaca tcaagactca tgctctcttc
     1621 attcaatatg tgtgccatag cttgcactaa aaaggcctta cctgcataga gcacctcata
     1681 atcaatatta catttatcaa atgcctttat atgctcctgg cattgctccc ta
//
LOCUS       DAOQWT010000382         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_83764_length_1731_cov_3.525060, whole genome shotgun sequence.
ACCESSION   DAOQWT010000382 DAOQWT010000000
VERSION     DAOQWT010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83764_length_1731_cov_3.525060"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKJ95_07065"
     CDS             complement(<1..637)
                     /locus_tag="PKJ95_07065"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HNR66032.1"
                     /translation="MNKEMRKRLTGNNLNKTNYYGLENGAVNFTRKIVFIVLISLLFF
                     FLFETCLAQENITVTNIWYKKMPNFTRVTIKANRPIQEFESMYVGDPDRIVIDVYDAD
                     YNITELVKNTLFLNMGSVKQVRCGQIEADKARFVIDLFQQADYDLALDSTKHLLQINV
                     YDYKEFLSPEQQIFTVEPLAAEEIRRIKEKELAIVPSLVDQVTIPITMNLKE"
     gene            complement(630..1334)
                     /locus_tag="PKJ95_07070"
     CDS             complement(630..1334)
                     /locus_tag="PKJ95_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66033.1"
                     /translation="MSRLNRILLVIVIILAAATALVWFKPDLFSSFSLPSLSSLNPFS
                     RGKSTAVGTDTATAQRPPVESTPDVVPSDIPLSVATSPVVESPVIEEEPEEELNTFEK
                     EIKQRHSSYETKIYTYQPYEPPVLRNPFQRMVSSVYVEEGERRLAEELSSAEDIRRFV
                     TPELPPGTKFSGLISSGDIKLAIIEMDEETYIAKEGDLIDDKYLVKLIQEDKVILDIN
                     GYEIAQKLGGEEDINE"
     gene            complement(1339..>1731)
                     /gene="pilO"
                     /locus_tag="PKJ95_07075"
     CDS             complement(1339..>1731)
                     /gene="pilO"
                     /locus_tag="PKJ95_07075"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNR66034.1"
                     /translation="KRIYARLGELRKEYQDLIEKMQFMEVLLPKEKEIPEFLVMLQET
                     MDEFNIDFSNFSPQSIIKEQDAIYARLPITMTYYANYFEVIRFLDRLENFPRIVDVRE
                     LSLTPDREKAENVNVTMTMFTYVLMKGN"
BASE COUNT          521 a          364 c          271 g          575 t
ORIGIN      
        1 tttctttcag gttcatggtg atcggtatgg taacctggtc taccagggaa ggcactattg
       61 ccaattcctt ttcttttatc cttctaatct cctcggcagc aagaggttca acggtaaaga
      121 tttgctgttc aggagaaaga aattctttat aatcatagac attaatctgc aggagatgtt
      181 ttgtgctgtc taaagccagg tcataatctg cctgttggaa taaatcgata acaaatcttg
      241 ccttatcagc ttcaatctga ccacatctaa cctgtttaac agaacccata ttcaaaaata
      301 acgtgttctt taccaattct gtaatattat agtctgcatc atacacatca ataacaatcc
      361 tgtcagggtc tcctacatac attgactcaa attcctgaat agggcgatta gctttgatag
      421 ttaccctggt aaagttgggc atcttcttat accagatatt ggttacggtt atattctcct
      481 gagcaagaca ggtttcaaat aaaaagaaaa ataataaaga aatcaaaaca ataaaaacta
      541 tttttctggt aaagttaacg gcaccatttt ccagaccgta ataattggtt ttgttaaggt
      601 tattacctgt taatcttttt ctcatttctt tattcattaa tatcctcctc acctcccaat
      661 ttttgtgcaa tctcataccc attaatatct aaaataactt tatcttcctg aattaattta
      721 actaaatatt tatcatcaat aagatcgcct tcttttgcaa tataggtctc ttcatccatt
      781 tcaataattg ccaatttgat atcaccggaa gaaattaatc cggaaaattt agttcccggg
      841 ggcagttccg gtgtaacaaa cctgcggata tcttctgcgc tgcttaattc ttccgccagc
      901 ctcctctccc cctcttctac atacactgaa ctgaccattc tctgaaaagg gtttcgcaac
      961 accggtggtt catatggctg ataggtatag attttagttt cataactact atgtcgttgt
     1021 ttaatctctt tttcaaaagt attcaattcc tcttctggtt cctcttcgat cacaggactt
     1081 tctacaacag gagatgtagc tacacttaaa ggaatatctg aaggcacaac atctggtgtg
     1141 ctctccaccg ggggtctctg ggctgtagca gtatcagttc ccaccgctgt actttttcct
     1201 ctgctaaaag ggttcagcga cgataatgaa ggtagagaaa acgaagaaaa caaatccggt
     1261 ttaaaccaga ccaaagcggt cgctgctgcc agaataatca caataactaa taatatacga
     1321 tttaatctac tcaaattatc aatttccttt cattaaaaca taggtaaaca tagtcatagt
     1381 gacattgaca ttctcagctt tttctctgtc cggagtaagg ctcagttctc taacatcaac
     1441 aattctgggg aaattttcta atctgtctaa aaacctgatt acttcaaaat aattagcata
     1501 ataagtcata gttattggca aacgggcata gatagcatcc tgttccttaa ttatactctg
     1561 tggagaaaaa ttggaaaaat ctatattgaa ttcatccata gtttcctgca acataaccag
     1621 gaattcgggt atctcttttt cttttggtaa aagcacttcc ataaattgca tcttctctat
     1681 taaatcctga tattcctttc tcaattcacc taagcgggca tagattcttt t
//
LOCUS       DAOQWT010000383         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_83963_length_1729_cov_2.448029, whole genome shotgun sequence.
ACCESSION   DAOQWT010000383 DAOQWT010000000
VERSION     DAOQWT010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83963_length_1729_cov_2.448029"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PKJ95_07080"
     CDS             <1..513
                     /locus_tag="PKJ95_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNR66035.1"
                     /translation="ALLDIYTILKEKGSLEGHTITFLGDLKYGRTVHSLGYFMTMYKN
                     KMIFVSPESLRMPEELTADFRSRGAEIEETDNVSEALSRSDIVYVTRIQRERFNNPEE
                     YEKLKGSYIIDRNTINKAKKGITVLHPLPRVDEISTDLDDYEGAAYFRQAHNGIFVRM
                     ALLALVSGVR"
     gene            592..1395
                     /locus_tag="PKJ95_07085"
     CDS             592..1395
                     /locus_tag="PKJ95_07085"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNR66036.1"
                     /translation="MKSAENKPLFVTGKVLENRNINGDYFEIVIEAPDIARNARPGQF
                     VMLSRWNINNLFLKRPFSFYQIDAEQGRFAILYKKIGKGTEILSQSKKADQVELIGSC
                     GNGFSLPENVGKIALVARGIGVATLLPLAIEGKKKGLEIYSFLSAREKNLLLGPDKLG
                     PLSSHIFYTTDDDWKGTDGKVTFFLEELLKNNRTDFEAVYVCGSKRLARHIRELQKEY
                     HFAAYISLEERMGCGIGACKGCVINTIYGYQRVCKEGPVFPLEEVILNE"
     gene            1388..>1729
                     /locus_tag="PKJ95_07090"
     CDS             1388..>1729
                     /locus_tag="PKJ95_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNR66037.1"
                     /translation="MNNKVDLSINLGNLQLKNPVMPASGTFDIRDTILNKEQLNSLGA
                     IITKSIASHKRLGNPGPRIWETEAGMMNAVGIPAEGFEYFKENILPLLEDFCVPIIVS
                     MVGDSITDFCQL"
BASE COUNT          571 a          278 c          368 g          512 t
ORIGIN      
        1 gctctcttgg atatctatac tatcctaaaa gagaagggtt ctttagaagg acataccata
       61 actttcttag gagatttaaa atatggtaga actgttcatt ccctgggtta ctttatgact
      121 atgtataaga ataaaatgat ttttgtttcc ccggaatctc ttcgtatgcc cgaagaatta
      181 acggctgatt ttagaagtcg tggagctgaa atagaagaaa ctgataatgt gtcagaagcc
      241 ctttccagga gtgatatagt ttatgttacc cgtatacaga gagaaagatt taataatcct
      301 gaagaatatg aaaaactaaa aggttcctac attattgacc ggaacactat caataaagct
      361 aaaaaaggaa ttactgttct ccatcccctg ccaagggtag acgagatttc aactgatctt
      421 gatgattatg aaggagcagc atatttccgc caggctcata atggaatctt tgttagaatg
      481 gctttgctag cactggtcag tggtgtaaga taaaaagttt gtttggttcc ttttatttcc
      541 ttgactggct tataaaaaga acctataaaa aatgaaaaga ttgggaaatg aatgaaatct
      601 gctgaaaata agcctttatt tgttacaggt aaagtattag agaatagaaa tattaatggt
      661 gattattttg aaatagttat tgaggcacct gatatagcca ggaatgcacg accaggccaa
      721 tttgtaatgc tctccaggtg gaacattaat aacctttttc taaagagacc attcagtttt
      781 tatcagattg atgctgaaca gggaagattt gctatcttat ataaaaagat cgggaagggt
      841 actgaaatat tatcgcaatc aaagaaagca gaccaggtag aattgattgg gtcctgtgga
      901 aatggttttt cccttcctga aaatgtaggg aaaattgccc tggttgcccg gggaatagga
      961 gtagctactc ttcttccttt agccatagag ggtaaaaaga agggattaga gatttacagt
     1021 ttcctgtccg cccgagaaaa aaacttatta ctgggaccag ataaactggg gccactctct
     1081 tcccatattt tttataccac agatgatgat tggaaaggaa cagatgggaa ggtgactttt
     1141 ttcttagaag aattacttaa aaataacaga acggattttg aagccgttta tgtttgcggt
     1201 tcaaaaaggt tagcaaggca tataagagaa ttacagaaag aataccattt tgccgcttat
     1261 atcagcctgg aagaacgaat gggctgtggg ataggtgcct gtaaaggttg cgtcataaat
     1321 actatttatg gttatcaaag agtttgtaaa gaagggccgg tttttccttt agaagaggta
     1381 attttaaatg aataataaag tggacttgtc aataaactta ggcaatctac agttaaaaaa
     1441 tccagttatg cctgcctctg gtacttttga tatcagagat actatactca acaaagaaca
     1501 gctgaactct ttaggtgcca tcatcaccaa aagcattgcc tcacataaaa gactgggtaa
     1561 tcccggtccc cgcatctggg aaacagaagc cggaatgatg aatgcggtag gaattcctgc
     1621 tgaaggattt gaatacttta aagaaaacat attaccctta ttagaagact tttgtgtacc
     1681 aattattgta agtatggtgg gagactccat tactgatttc tgccagctg
//
LOCUS       DAOQWT010000384         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_84099_length_1727_cov_2.698565, whole genome shotgun sequence.
ACCESSION   DAOQWT010000384 DAOQWT010000000
VERSION     DAOQWT010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84099_length_1727_cov_2.698565"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1426
                     /locus_tag="PKJ95_07095"
     CDS             116..1426
                     /locus_tag="PKJ95_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR66038.1"
                     /translation="MYLLILFKKEKLVWVTFFITLIFCLFLLFEKNVCSSEILNLDLE
                     ECIEIALENNLGYLISQSKVGVKDEQIKEVRGAKRVNAKFQGGYIRMNEAPDMESIAE
                     GDYSYLYSSGTGVPYISVNVSKVLYSGGKLETLIDLAKAEQSISVNDLEINRQELIFK
                     VTEAYYRVLQAEGLKRVSEQALTQIKAHLETSRSLLAEGLIAPIDLNRINSQLSNLEH
                     NLIRAENGYMMAVFYLNSAMGIPLQTTINLQNSLSYEAHIISLEEALEAALQNRPEIH
                     NINQQRVIMEGMVDIAKSNKKPQIVMNMESGLAGWKVAVLGEVPIFDGGVNKSKIKQA
                     EMNLSLVEQSKKQAEQLIELEIRSVYLSMKEAEKLFKVTEQGIKDSRESFRIAQVKYN
                     EGVATNIEVIDAQSALIEAETNHLNALYEYNTSWASLIKAMGLF"
     gene            1490..>1727
                     /locus_tag="PKJ95_07100"
     CDS             1490..>1727
                     /locus_tag="PKJ95_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66039.1"
                     /translation="MRVTKRRTFYIIIIIVVLLLILQTTNRIFKKRQEQTSLPQVQAE
                     RIVPVQVARVQRGEIKSFLTVSGLVEPKEIVRVFA"
BASE COUNT          591 a          263 c          375 g          498 t
ORIGIN      
        1 ggcactctca gtctggaaaa agatgaaaga gatggaaaaa gaaaataaga acagtaggtt
       61 ttattaaata cattttatta ttacctaatc tttagatttg aaaggaggtt tgtctatgta
      121 tctactcata ttatttaaaa aagaaaagtt ggtttgggtt acttttttta taactttgat
      181 tttctgtctt tttttattat tcgagaaaaa tgtttgttct tcagaaatct tgaatctgga
      241 tttagaagaa tgtatcgaaa ttgctttaga aaacaatctt ggttatttga tttcccagag
      301 taaggtggga gtaaaagatg aacagataaa agaagtcagg ggtgccaaac gggtgaacgc
      361 caaatttcag ggcggctata ttagaatgaa tgaagctccg gatatggaaa gcattgccga
      421 aggcgattat agttacctct actcctcagg tactggagtt ccctatattt cggtaaatgt
      481 cagcaaagta ctgtatagtg gtggtaagct ggaaactctt attgatctgg ctaaggcgga
      541 acagtctata tcggtgaatg acctggaaat aaacaggcag gaactaatct ttaaagtaac
      601 cgaagcttat tatcgggtat tacaggcaga aggattgaag agagtgagtg aacaggcttt
      661 aactcagatt aaggcacatc tagaaacctc tcggtccctg ctggcagaag gattgattgc
      721 gcccattgat ctgaaccgta ttaattcaca actttctaac ctggaacaca atctgattag
      781 agcagaaaat ggttatatga tggcggtatt ttatctgaat tctgctatgg gaatcccctt
      841 acagactacc atcaatttgc agaacagtct ttcctatgaa gcccatatta tttcactgga
      901 agaggcattg gaagctgcac tacagaacag gcccgagata cacaatatta atcaacaaag
      961 ggttataatg gaaggaatgg tagatatcgc aaaaagtaat aaaaaaccac agattgtgat
     1021 gaatatggag tcaggattag ccgggtggaa ggtggcagtc ctgggagaag tacctatctt
     1081 tgatggcgga gtgaataaat caaaaatcaa gcaagccgaa atgaatctat ctctggtaga
     1141 gcagagtaaa aaacaggcag aacagttgat agaacttgaa atacgttctg tttatctgag
     1201 catgaaagag gcagagaagt tatttaaggt gactgaacag ggaataaaag attccaggga
     1261 aagctttaga attgctcagg tgaaatataa tgaaggggtt gctaccaata tcgaagtaat
     1321 tgatgcccaa agtgctttaa ttgaggcaga aactaatcat ttaaatgccc tgtatgagta
     1381 taacaccagc tgggcttctt taataaaagc tatgggttta ttttaaaaat attttaattt
     1441 aaatactaag ttaatttttt caagaatttt caggaaggga attgttttta tgagagtaac
     1501 caaacgcagg acattttaca taattatcat aatagtggta ttattactta tccttcagac
     1561 aactaaccgc atttttaaaa aaagacagga acaaacctca ttacctcaag tgcaggcaga
     1621 aagaatagtt ccggtacagg ttgcccgggt tcagcgtggt gaaattaaat cctttttaac
     1681 cgtttctgga ttagtagaac caaaagaaat cgtacgagtc tttgcca
//
LOCUS       DAOQWT010000385         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_85318_length_1711_cov_2.820048, whole genome shotgun sequence.
ACCESSION   DAOQWT010000385 DAOQWT010000000
VERSION     DAOQWT010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85318_length_1711_cov_2.820048"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1695)
                     /locus_tag="PKJ95_07105"
     CDS             complement(<1..1695)
                     /locus_tag="PKJ95_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66040.1"
                     /translation="MAAEGKIVLSQPDKPEINLSYQIAPLSLDPLLETLAYSVPLSGQ
                     VEGNGYIQGIWPLLEVDGVFQLEEIIYENYPLGQGEANFHLQPEQGQKTAYKNQEINF
                     DNFFTEIGHYYSLDLEHLKLQNETIEINAKGQAKMEEGNPFSLEIEFSHQKFEEMIEH
                     FYPAQENIKKFLPSRITGKISCTGNNDKQNFLLSSLLIPQKEEDNPPSRLEAVFTVDK
                     DGFNISDFKLIQSEGLLKAEGIIASDRSLDINFQANQLDLSNLFASLAIDEEIKGIMD
                     IKGFCIGTLEQPVVSIIAKIKEGYFREFYYENLHSELIWSSLTNQIEIKELTIDLEEK
                     YQITARGNIPAESFIFPKEEELELAPTYSGIPLDFQINMEKTDLNILKIFWKDTFSEI
                     TGTLGLKLSFSGTADNPIVNGKIDIYQGQVQFMGLPVQIEEINDSIRIINNRVTMPSI
                     SFRAYQNRFILSGEFELIHFLPNNLSFIIKNEDKRIIYQDILDSEADLGLLIKNSILS
                     PKISGQVLFSNGILNVNNLLKMREDMDLSFSPSLPNSNSLEQLEVNIELLEPFKLKM"
BASE COUNT          499 a          326 c          300 g          586 t
ORIGIN      
        1 cattttcagt ttaaagggtt cgagtaattc aatatttacc tccagctgct ctaatgaatt
       61 gctattcggc aaggaagggg agaatgaaag atccatatcc tctctcatct ttaataaatt
      121 gttaacgttc agtattccat tagaaaacag cacctgcccg cttatctttg gagataatat
      181 agaatttttg attagcagtc ccaaatctgc ttcgctgtcc agaatatctt gatagatgat
      241 tctcttatcc tcattcttta tgataaaaga caggttattt ggtagaaaat gaattaattc
      301 aaattcaccc gataagatga atcgattctg gtaagctcta aaagatatag aaggcatagt
      361 gactctatta tttattattc ttatgctgtc attgatttct tcaatctgta ccggtaatcc
      421 cataaactga acctgaccct gataaatatc aatctttccg tttactatag gattatcagc
      481 tgtgccgctg aaagagagct tcaggccaag tgttcccgta atttccgaga atgtatcttt
      541 ccagaatatc tttaatatat tgaggtctgt tttttccata ttaatctgaa aatcaagagg
      601 tatacccgaa taggtaggtg ccagttctaa ttcttcttct ttagggaaaa taaagctttc
      661 cgcgggaata tttccccggg cagttatctg gtatttttct tccaggtcaa tggttaactc
      721 cttaatttct atctgattgg ttaagctgct ccagatgagc tcactgtgta aattctcata
      781 gtaaaattcc cggaaataac cttctttgat tttagcaatt atggaaacta caggctgttc
      841 taatgttccg atacaaaatc ccttgatgtc catgatacct tttatttctt catcaatagc
      901 cagtgaggca aatagatttg acagatctaa ttgattagcc tgaaaattaa tatctaaact
      961 cctgtcggaa gcaattatcc cttctgcttt cagcaaacct tctgattgaa ttaacttaaa
     1021 atcggatata ttgaaaccat ctttatctac ggtaaacact gcttctaacc tggagggagg
     1081 attatcttcc tctttctggg gtataagcag tgaggatagc aaaaaatttt gtttatcatt
     1141 attgccggtg cagcttatct ttcctgttat acgggaaggc aggaatttct ttatattttc
     1201 ctgggcagga taaaaatgtt caatcatctc ttcaaatttt tgatgtgaaa attcaatttc
     1261 cagtgagaag ggattccctt cttccatttt agcctggccc ttcgcattta tctcaatcgt
     1321 ctcattttgt aatttgaggt gctctaaatc aagagaataa taatgaccaa tttcagtaaa
     1381 gaaattgtca aaatttattt cctgattttt ataagctgtc ttctgaccct gttctggctg
     1441 taagtgaaaa tttgcctcac cctggcccaa gggatagttt tcataaatta tctcttctag
     1501 ctggaagact ccgtctactt ctaatagagg ccagataccc tgtatataac cattaccctc
     1561 aacctgaccg gataagggga cagaataagc taatgtttcc agtaaaggat ctagagagag
     1621 aggggctatc tggtaagaaa gattaatttc aggtttatca ggttgagata aaacaatctt
     1681 tccctctgcg gccattttta aatcctggtt t
//
LOCUS       DAOQWT010000386         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_85322_length_1711_cov_2.758454, whole genome shotgun sequence.
ACCESSION   DAOQWT010000386 DAOQWT010000000
VERSION     DAOQWT010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85322_length_1711_cov_2.758454"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PKJ95_07110"
     CDS             complement(<1..651)
                     /locus_tag="PKJ95_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNR66041.1"
                     /translation="MLNKKHAERLGELLRKEEIDVVIIGPSVDLYYLTGFNVHVCERF
                     QAFFFQSDGKYYHISPQLYFEEAQTNLGQDTDIFMWRDSENFLEAIEAVNQKYGLEGK
                     TIAINNAIRGVDLIYIQTKLKADFVNGNDLMETLRVRKDESEVEKLRKAAHIADEVVG
                     EVIEYIKPGMTERNVKEKIEELFTQKGVPMSFNPIVASGPNSSMPHYCDDSRVIQEK"
     gene            complement(705..>1711)
                     /locus_tag="PKJ95_07115"
     CDS             complement(705..>1711)
                     /locus_tag="PKJ95_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR66042.1"
                     /translation="GFAGFMWSLLLSTPFAILFGIITAMLLNRTKGQEMITSMIAGFF
                     ANGVYQFILLFLVGTLIPIKNPDLVLSRGIGIRNTIDLATKTGLKYALDDVLKYPLFQ
                     VLAIIAAVIIIGLVVNYFLKKRSGYEGIDNFKFITQLIIMGAILGISIYVIRSGSLLK
                     SVKLPILTTIFIAAIWGFNELIVKTKLGQDFRTIGHDMEIAKISGIDVDRTRIIAMVI
                     STVFAAWGQIIFLQNIGTLSTYGSHVQIATFSIAAILLGGASVTKATSGQAILGIILF
                     HTLFIVSPKAGNNLFGDAQIGEFFRAFISYGVIGVALGLHAWNKRIQAMRKQLDIDVI
                     "
BASE COUNT          496 a          370 c          318 g          527 t
ORIGIN      
        1 cttttcctga attaccctgg agtcatcaca ataatggggc atagagctgt tgggaccaga
       61 ggcaacaata ggattaaaag acattgggac acccttttga gtgaataatt cttctatctt
      121 ttccttgacg tttctttcgg tcatccccgg tttaatatat tctattacct cacctaccac
      181 ctcatcagct atatgggcag ccttccttaa tttttctact tcgctctcat cttttctcac
      241 cctcagtgtt tccatcaaat cattgccatt cacaaaatct gccttgagct ttgtctggat
      301 atatatcagg tctacgcctc tgatggcgtt gttgatggct attgttttcc cttccagccc
      361 atatttttga ttaactgcct ctatggcctc caggaaattt tcactgtctc tccacataaa
      421 gatatcggta tcttgcccca gattagtctg agcctcttca aaatagagtt ggggagaaat
      481 gtgataatat tttccatcgg attgaaagaa gaaagcctgg aatctttcac agacatggac
      541 attaaaaccg gttaaataat agaggtcaac agagggacct ataatgacta cgtcaatctc
      601 ttcttttcgt aaaagttcac caagtctttc tgcgtgtttt ttatttagca taggatcttt
      661 cctttctatt aatattgagc tattttcatt ctagcaatag gtagttaaat tacatcgata
      721 tccaattgtt ttctcatagc ctgaattctt ttgttccagg catgcagtcc cagtgcaact
      781 cctatgactc cataagagat aaaggctcta aagaattcac ctatctgggc atccccgaag
      841 aggttgttgc cagcctttgg tgatactatg aagagggtat ggaacaggat aatacccaaa
      901 atagcctgcc cgctggtagc tttggtgaca gacgctcccc ctaaaagtat ggcggcaata
      961 gaaaaggtgg caatctgcac atgacttcca taagtggaga gagtgccaat gttttgtagg
     1021 aaaataatct gtccccaggc tgcaaaaaca gtagaaatta ccatagcaat aattcttgtt
     1081 ctgtctacat ctatccctga aattttggcg atttccatat catgaccgat ggtcctaaaa
     1141 tcctgtccta gcttggtctt gacaatcaat tcattaaaac cccatattgc ggcaataaat
     1201 atagtcgtta aaatggggag tttcactgat tttaagagcg aaccagacct gatgacatag
     1261 atacttattc ccagaattgc tcccataata ataagctggg taataaactt aaaattatca
     1321 atcccctcat aaccacttct ttttttcagg aaatagttta caactaaacc tattatgatc
     1381 acagcggcta ttatggctaa gacctgaaag agagggtatt ttaatacatc gtcaagagca
     1441 tatttcagtc cggtttttgt ggccaggtct atggtatttc tgataccaat tcccctgctc
     1501 agcaccagat ccggattttt tatgggaatt aatgtaccca ctaaaaagag aaggatgaat
     1561 tgatagactc cattggcaaa aaacccggca atcatgctgg taatcatttc ctgacccttt
     1621 gttctgttta agagcattgc cgtaattatg ccaaagagga tggcaaaagg agtggaaagc
     1681 aaaaggctcc acataaatcc ggcaaaaccg g
//
LOCUS       DAOQWT010000387         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_85486_length_1709_cov_2.642684, whole genome shotgun sequence.
ACCESSION   DAOQWT010000387 DAOQWT010000000
VERSION     DAOQWT010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85486_length_1709_cov_2.642684"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PKJ95_07120"
     CDS             complement(<1..884)
                     /locus_tag="PKJ95_07120"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNR66043.1"
                     /translation="MTWVYSPRYGKNLFVAEEKSLWGEKLLKVIIPDEKRIENIPSGE
                     VISLEQKESKSKDHIIYSSCAAKIADSIGTDMVISPFGGNLIPLPHQLNALTKVLSGD
                     MVRFLLADEVGLGKTIEAGMIFKELKIRGLVKRVLIVAPKGLVLQWVGEMKKHFNETF
                     HLIIPGKISPDKLYTVLTDSGEEIFSNRLNYWKNFDQVICSMDSVKPLEKRKGWSEEE
                     IERYNRERFDNLLQAGWDLIIIDESHRMAGSSHTVSRHTLGRNLAYCSPYLLLLSATP
                     HSGKSDSFVGGQHAAVQRL"
     gene            complement(1005..>1709)
                     /locus_tag="PKJ95_07125"
     CDS             complement(1005..>1709)
                     /locus_tag="PKJ95_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNR66044.1"
                     /translation="ETAYLYALIEFQSSPDKYIALRVLSYMLLFYEDLIATGQVKDKL
                     PPVFPLVIYTGEQKCNAPLNIEELIEQPYKRLRKYVPCFTYHMLDLHGLKEEQLSNLY
                     RLDNILAAFFNLVTKIRTEEIIEAEKILIEMVDFTSELGRFIKIWLGTYLKKHNIPFN
                     IDVEGGSPMLATAIKNSIQEAQEKGMEEGIEKGKLEGKLEGKLDTVKKMLSRNYPVEE
                     IAEVTGLSLEEIEKIK"
BASE COUNT          478 a          356 c          283 g          592 t
ORIGIN      
        1 aggcgttgaa cggcggcatg ctgacctcca acaaaggaat cgcttttccc ggaatgaggt
       61 gttgctgaaa gaaggagcag gtaaggtgaa caatatgcaa gatttctgcc cagtgtatgg
      121 cgggatactg tgtgggaaga acctgccatt ctgtgggatt catctattat gataagatcc
      181 catcctgcct gaagaaggtt gtcaaatctt tctctgttat atctttctat ttcttcttca
      241 gaccagcctt ttctcttttc cagaggtttc actgaatcca tagaacagat aacctgatcg
      301 aaattcttcc agtaattcaa tctgttacta aaaatttcct ctccactatc agtaagaact
      361 gtatagagtt tatccggaga tattttaccg ggaattatca gatgaaaagt ttcattaaag
      421 tgttttttca tttctcccac ccattgaagg acaagtccct ttggtgccac aataagaact
      481 ctttttacaa gtccccttat tttaagttcc ttaaatatca tgcctgcttc aatggttttg
      541 ccgagtccca cttcatcggc aaggagaaat ctcaccatat cacctgaaag tactttagta
      601 agagcattga gctgatgcgg aaggggtatg aggttccctc caaagggaga aattaccata
      661 tctgttccta tggaatctgc tatttttgct gcacaggaag aataaataat atggtctttt
      721 gatttgcttt ctttctgctc aagagaaatg acttctcctg aagggatatt ttctattctc
      781 ttttcatcag ggataattac ctttaatagc ttttctcccc agagggattt ttcttctgct
      841 acaaaaaggt ttttaccgta acgtgggctg taaacccagg tcatggatta ccaccagcct
      901 tgttattttg tttccataat ggaaaataaa aaaagaggca tatgccacta tttttaatag
      961 caacatagga caccctcatt ctggatcaat tccaaagtag aagattattt tattttttca
     1021 atttcttcca gagataatcc tgttacctct gctatctctt ctacaggata atttctggat
     1081 aacatctttt ttacagtatc aagcttacct tcaagtttcc cctctaattt ccctttctct
     1141 attccttctt ccattccttt ctcctgagct tcctgaatac tgtttttgat agctgtggca
     1201 agcataggac tccctccttc cacatcaata ttaaatggta tattatgttt tttaagatag
     1261 gtaccaagcc atatctttat aaaacgtccc agttcacttg taaaatccac catttctatt
     1321 aatatctttt ccgcttctat aatttcctct gttctaatct ttgttaccag attaaagaat
     1381 gccgctaata tgttatccag tctatataaa ttacttaact gttcttcttt taatccatgt
     1441 aagtccagca tatgataggt aaaacatgga acatattttc ttaatcgttt ataaggctgc
     1501 tctattaatt cttctatatt taaaggagca ttgcatttct gttcacccgt atatattacc
     1561 agtgggaaca ccggaggcag tttatcttta acctgcccgg tagctatcaa atcttcatag
     1621 aataacagca tataactgag aactcttaaa gcaatgtatt tatcggggct actctggaac
     1681 tcgatgagag catataaata agctgtttc
//
LOCUS       DAOQWT010000388         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_85617_length_1707_cov_3.763317, whole genome shotgun sequence.
ACCESSION   DAOQWT010000388 DAOQWT010000000
VERSION     DAOQWT010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85617_length_1707_cov_3.763317"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /gene="glmM"
                     /locus_tag="PKJ95_07130"
     CDS             <1..1364
                     /gene="glmM"
                     /locus_tag="PKJ95_07130"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNR66045.1"
                     /translation="NTLFGTDGIRGLANQYPMNAELAFYLGQTIGYYNKNKKNKDILL
                     GKDTRISGDMLEAALSAGLCSTGMDVLNVGIATTPAVGYLTKYYHANMGVVISASHNP
                     YYDNGIKIFQADGLKINKRIEREIEKILFQEKYKKIQNTGSAIGRVIPLNDARKIYLE
                     HVMDTIPQDFKKPGYRIVLDCANGSSSQIIPELLTKLKISFNTINDTPNGININHKCG
                     STYISNLQEEVFKKKADIGLAFDGDADRVLAVDEKGSIVNGDQIMVIYANTFIKEGKL
                     GNKVIVTTHMSNLGFDETINEIGGSVIRTDIGDKYVLKKMLSTNSLLGGEQSGHIIFL
                     PYSPNGDGIITALQLLYALNKNNEPVSIQAGRMKKYFQKLLNYKISDKKYFTQNEKFH
                     EMNKVIQNYFKKEGRVLIRFSGTEDKIRILLESKEEKKIKECKKVIDDYIANISSFNK
                     NDF"
     gene            1423..>1707
                     /gene="acpS"
                     /locus_tag="PKJ95_07135"
     CDS             1423..>1707
                     /gene="acpS"
                     /locus_tag="PKJ95_07135"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNR66046.1"
                     /translation="MIIGTGIDIVDIDRIKKIIKKWDLHFLNKVYTLDEKYYCEQKKD
                     SCFQSFAGYFAAKEAWVKAIGTGMKNIKWKEIEIKKDPLGRPFIKLYGLAM"
BASE COUNT          663 a          213 c          326 g          505 t
ORIGIN      
        1 tgaatacatt atttggtact gacggaatca gaggattggc aaatcagtat cctatgaatg
       61 ctgaattagc attttatttg gggcaaacca ttggctacta taataaaaat aaaaaaaata
      121 aagatatatt actgggcaaa gacaccagaa tttctggtga tatgttagaa gctgcgctct
      181 cggcaggact gtgttcaacg ggtatggatg tgctaaatgt cggtattgcc actacacctg
      241 cagtaggata cctaacaaag tattatcatg ccaatatggg ggttgttatt tctgcctccc
      301 ataatcctta ttatgataat gggattaaaa tattccaggc tgacggatta aaaattaata
      361 aaagaataga aagagaaatt gaaaaaatac tctttcaaga gaaatataaa aaaattcaga
      421 ataccggtag tgctattggc agagtaatac cattaaacga tgcccggaaa atctacttag
      481 aacatgtaat ggacactata cctcaagatt ttaaaaaacc agggtacagg attgttttgg
      541 attgtgccaa tggttcctcc agtcagatta ttcccgaatt attgactaaa ttgaaaatta
      601 gttttaatac tataaatgat actccaaatg gaattaatat taaccacaaa tgtggttcaa
      661 cttatattag caatttacaa gaggaggttt ttaagaaaaa agcggatatt gggcttgctt
      721 ttgatggtga tgcagacaga gtactggcag tagatgaaaa agggtcaata gttaacgggg
      781 accaaataat ggttatttat gccaatactt tcattaaaga agggaaattg ggtaacaagg
      841 taattgtcac tactcacatg agcaatttag gttttgatga aaccattaat gagataggtg
      901 gttctgttat tagaaccgat attggagata aatatgtctt aaaaaaaatg ttaagtacca
      961 actctctatt gggtggtgaa caatccggac atattatatt tttaccatac agtcccaatg
     1021 gagatggtat cataactgcc ctacaactat tgtatgcttt aaataaaaat aatgaacctg
     1081 tctcgattca ggctggacgg atgaaaaaat atttccaaaa attgcttaat tataagatat
     1141 ctgataaaaa atattttact caaaatgaaa aatttcatga gatgaacaaa gtaattcaaa
     1201 attatttcaa aaaagaggga agggtcttaa taagattttc agggactgag gataaaataa
     1261 ggattttgtt ggagagtaaa gaagaaaaaa agattaaaga atgtaaaaaa gttatagatg
     1321 attatatagc taacatatca agttttaata aaaatgattt ttagtattct tttaaaatcg
     1381 tgtttcttat tggaacaaaa aagtaggata gcaggtaaaa gaatgataat aggaacagga
     1441 attgatatag tagatataga taggattaaa aaaatcataa aaaaatggga ccttcatttt
     1501 ttaaacaaag tatatacttt ggacgaaaaa tactattgtg aacaaaaaaa agacagctgc
     1561 tttcagtctt ttgctggata ttttgcagca aaagaagctt gggtaaaagc tattggtaca
     1621 ggtatgaaaa atataaaatg gaaagaaatc gaaataaaaa aagatccttt agggagacca
     1681 tttattaaac tttacggtct ggctatg
//
LOCUS       DAOQWT010000389         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_85623_length_1707_cov_3.596852, whole genome shotgun sequence.
ACCESSION   DAOQWT010000389 DAOQWT010000000
VERSION     DAOQWT010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85623_length_1707_cov_3.596852"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="PKJ95_07140"
     CDS             <1..1266
                     /locus_tag="PKJ95_07140"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNR66047.1"
                     /translation="ESRLVKKYKPYFNSQLKDDKSYPFIQITKGVDFPAIHLIRKSSK
                     TAEKKARYYGPFTDVETSRKAVQKLRNIFKIRNCSQRKFNSGKPCLDYQIGLCSAPCA
                     SKIEKGEYQKKIRECGLLLTGRHRSLLKSLRQEMKITAQLMLYEKAAKIRDTIKMIEK
                     VISQEKISKSYQSRLNIYLRDSVNEESLRVALRDLQQYLDLPVLPLSIEAYDISNIQG
                     KNAVGSLVYFHNGIPDKKKYKHFKIKYVPGINDYAMMREVLERRFGNCQSTEEGLPDL
                     ILVDGGKGQLNIVSQLLNELNLPLNLVSLAEREEQLFQLGRKEPVLLPGNSAAFFLIQ
                     RIRDEAHRFALSYHKKLRSKTIKDSLLDFIPGIGEKKKKLLLSKFKGIDSIRNASKEE
                     LKEIPGIGEKTAQKIKDILEARYDCKIQE"
     gene            1244..1699
                     /locus_tag="PKJ95_07145"
     CDS             1244..1699
                     /locus_tag="PKJ95_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNR66048.1"
                     /translation="MIVKFKNKTPQIDQTALVVENATLIGEVYVGKYSSIWFSAVLRA
                     DISLISIGDSTSIQDGTVLHVAENLPTKIGNYVTIGHGAILEGCIVEDTVLIGSGANI
                     LNGVNIVLTKGYTLTGFPKSLITIGNGLFLGIPIPFYIFVIFSFLPVVP"
BASE COUNT          602 a          268 c          349 g          488 t
ORIGIN      
        1 gagagcagac tggtcaaaaa gtataaacca tatttcaata gtcagctaaa agatgataaa
       61 agttatccct ttatccagat aactaaagga gttgattttc cggctattca tctgatcaga
      121 aaaagcagta aaactgctga gaagaaagca cgctattatg gcccctttac cgatgtggaa
      181 accagcagga aagcagtgca gaaattaaga aatattttta aaatcagaaa ttgcagtcaa
      241 cgaaaattca attcaggaaa accctgcctt gattaccaga ttggtttgtg ctcagccccc
      301 tgtgccagta agatagaaaa gggtgaatac caaaaaaaaa ttagagaatg tggtttgttg
      361 ctgactggca gacacagatc cctcttaaaa agtttaaggc aggaaatgaa aataacggct
      421 cagttgatgt tatatgagaa agcggcaaaa attagggata ccataaagat gattgaaaaa
      481 gtcatcagtc aggaaaagat tagtaaatca tatcagagca gattaaatat ctatctccgg
      541 gatagtgtta atgaggaatc tttaagggta gcccttagag atttgcagca atacctggac
      601 ctgcctgtat tgcccctctc tatagaagcc tatgatattt ccaatattca aggaaaaaat
      661 gcagttggtt ctttagtcta ttttcacaat ggaatcccgg acaagaaaaa atataaacat
      721 tttaaaataa aatatgtacc aggaattaat gattatgcca tgatgagaga ggttttagaa
      781 cgacgatttg gcaattgtca atcaacagaa gaaggattgc ccgatttaat acttgttgat
      841 ggagggaaag gccagttgaa catagtatca cagctattga atgaattaaa tttaccactc
      901 aatctggttt ctcttgcgga aagagaggag cagctatttc aactagggag aaaagaacct
      961 gtattgcttc ccggaaattc ggcagccttc tttctcattc agaggataag ggatgaagcg
     1021 catcgctttg cactgagtta ccataaaaag ctgaggagta aaactattaa ggattcattg
     1081 cttgatttta ttccgggcat tggtgaaaaa aagaaaaagc tgcttttatc aaaatttaaa
     1141 ggtattgact ctatcagaaa tgcctccaaa gaagaactta aagaaatacc tggaattgga
     1201 gaaaagacag ctcaaaaaat aaaggatata ttggaggcaa gatatgattg taaaattcaa
     1261 gaataaaaca ccacaaatag atcaaactgc tttagttgtt gaaaatgcca ctttaatcgg
     1321 tgaggtatat gtgggaaaat acagttctat ctggttttct gcggtattgc gtgctgatat
     1381 tagtttaatc tctattggag atagtaccag tatacaggat ggtacagtat tacatgtggc
     1441 agaaaactta cccactaaaa taggaaacta tgttacaatt ggacatgggg caattttaga
     1501 agggtgcata gtggaggata ctgttttaat tggttctgga gcaaatattt taaatggagt
     1561 aaatattgtt ctcaccaagg gatatactct aaccggtttc cctaagtctc taattaccat
     1621 aggaaatggc ttattccttg gaatcccaat accattttat atatttgtta ttttttcttt
     1681 tttacctgta gtaccgtaat tgctgat
//
LOCUS       DAOQWT010000390         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_85668_length_1707_cov_2.273608, whole genome shotgun sequence.
ACCESSION   DAOQWT010000390 DAOQWT010000000
VERSION     DAOQWT010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85668_length_1707_cov_2.273608"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PKJ95_07150"
     CDS             <1..466
                     /locus_tag="PKJ95_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNR66049.1"
                     /translation="KSEEYFQFKDVLYQVTEYLAKEIARDGEGATKLIEVEVKNALTD
                     IDAGKIAKAVAGSLLVKAAIYGHDPNWGRIFAAAGYSGAQIDVNKIDLFVKEKIVSDG
                     QPLPFSKDTVRKYLEESTEIKIIIDLKIGNGKATAWGCDLTHEYVNINTKYN"
     gene            498..1385
                     /gene="argB"
                     /locus_tag="PKJ95_07155"
     CDS             498..1385
                     /gene="argB"
                     /locus_tag="PKJ95_07155"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015790979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNR66050.1"
                     /translation="MLKNDTWRADILVEALPYIKKFWNKIVVIKYGGSAMEDKQLEKQ
                     ILKDIILLRYIGMKPIIVHGGGPEISQEMLKQNKTPQFINGLRVTDRDTMDITEMVLT
                     GKINTRLVAEINLCILEDQGNGIKGIGLSGEDAQLLVVKKYAHIDLGYVGEIIKINTE
                     ILTLLIEKDYIPVIATIGVDASGDRFNINADTVAGELAKVLKAEKLIFLSDVDGILRE
                     LGNVETLISSINVEEAKKLLESGQVQKGMIPKVESALGALEGGVKKVHFLNGKQVHSI
                     LLEIFTDKGIGTEFVSVDN"
     gene            1428..>1707
                     /locus_tag="PKJ95_07160"
     CDS             1428..>1707
                     /locus_tag="PKJ95_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR66051.1"
                     /translation="MESKELINIEQNFLIQTFKRPAMVLDHGRGIKVWDLEGKEYYDF
                     IGGIAVNSLGYSHPKIVQAICEQAGKIIHCSNLYYNAPQILLGKKLVEL"
BASE COUNT          618 a          226 c          390 g          473 t
ORIGIN      
        1 aaagagtgaa gaatactttc aattcaagga tgtcctttat caagttacag aatatctggc
       61 taaagagata gctagggatg gagagggtgc taccaagctt atcgaagtag aagtgaaaaa
      121 tgccctgact gatattgatg ctggtaaaat tgccaaagcg gttgcaggtt ctcttttagt
      181 caaagccgct atttacgggc atgaccccaa ttgggggcgt atctttgcag cagctggtta
      241 ttccggagcg caaattgatg taaacaaaat agatctattt gttaaagaaa agattgtttc
      301 agatggtcag ccactcccct tctcaaaaga tacggtacga aaatatttgg aagaatctac
      361 agaaataaaa ataatcattg atttaaagat tggtaatgga aaggcaactg cctggggttg
      421 cgatctgaca catgagtatg tcaatattaa tacgaaatat aattagaata taattaacaa
      481 aaaatagggg gtgaaaaatg ttaaaaaatg atacctggcg cgcagatatt ttagtagagg
      541 cattgcccta tattaaaaag ttctggaata aaattgtagt tattaaatat ggcggtagtg
      601 ctatggaaga taaacaactg gagaagcaaa tcttaaaaga tatcatatta ttacgttaca
      661 taggtatgaa accgataata gttcatggcg gtggtccgga gatatcacag gagatgctca
      721 aacagaataa aacccctcaa tttataaatg gattgagagt aaccgaccgt gatacaatgg
      781 atattacaga gatggtttta accggtaaga taaatacccg tctggtagct gaaattaatc
      841 tatgcatctt agaagatcag gggaatggga ttaaaggaat tggattgagc ggggaagatg
      901 cccaactgct tgtggtaaaa aaatatgctc atattgatct gggttatgta ggagaaatta
      961 taaaaataaa tacagaaata ttaactttac tcatagagaa ggattatata ccggtaattg
     1021 ctaccattgg cgtagatgcc agcggagata gatttaatat aaatgccgat acggtagcag
     1081 gggagctggc taaagtatta aaggcagaaa aattaatatt tttatccgat gtagatggaa
     1141 tattaagaga attgggaaat gttgaaaccc taatcagcag tattaatgtt gaagaagcaa
     1201 aaaaattatt ggaatctgga caggttcaaa aaggtatgat tcctaaggtt gaatcagctt
     1261 taggagcttt agaaggaggg gtcaaaaagg tgcatttctt gaatgggaaa caagtgcact
     1321 caattttgct ggagatattt accgataagg gaatcggaac tgaatttgtg tctgtcgaca
     1381 attaaaaaaa gtattatatg ccaaatttga ataggaggag gaataatatg gaaagtaaag
     1441 aattaattaa cattgaacaa aattttctaa ttcagacttt taaaagaccg gctatggttt
     1501 tagaccatgg aaggggaata aaggtctggg atttagaggg aaaagaatat tatgatttta
     1561 ttggtggtat tgcggtaaat agtctgggat acagccatcc taaaattgtg caggcaattt
     1621 gtgagcaggc cggtaaaatc atccattgtt ctaacctcta ctacaatgct ccgcaaatcc
     1681 tgttaggaaa aaaattggtg gaattat
//
LOCUS       DAOQWT010000391         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_85897_length_1704_cov_2.791389, whole genome shotgun sequence.
ACCESSION   DAOQWT010000391 DAOQWT010000000
VERSION     DAOQWT010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85897_length_1704_cov_2.791389"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /locus_tag="PKJ95_07165"
     CDS             complement(<1..1654)
                     /locus_tag="PKJ95_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66052.1"
                     /translation="MSIEEAFNEIATWQTTCHYDKTPEDRRKRLKPNIKSIYKMNFKF
                     YCAYGRELKLPCNQSCPLYNKKKGIREIKAETPCNNALTIEEARKEILNTLEENLKTR
                     DIDKKNVIIKSPPGIGKSTIAINFYTEKHRKNRHNIFILVQARIDHLKELQDRGELPA
                     DAWQIIRGRKQIKEEYKEGEEPGNCYKADEINELCKKRGFHHIPYKCELGCEHKDRCK
                     NSPCMYLGQWDTGKNMAMTHEMLLLGGSNIKKVTRVIIDEIPLSSFIEKIEVPEIEIT
                     QSVIELERQYMKAPYFYIYKTLYAIAEGLKMAGQNKVLNGSDFLKRFEFDFSRLAPPG
                     KDLDFILSVKPSDIWESKEYYWRQDFCSILQAEIKDWRTHGDSYNSNIYLKYHEKHKN
                     IEFYFLRKRELKEKIKNAGIIALDATTDKSIWEQATGTLMQEKIINVSLPEGSKVIQV
                     YSGRYGKKYHFPTKEGQKTTNPVILQKEQAIIDLLKDKAFFGGWQEAKKNLTFPDAEC
                     FGHFYGTRGTNRFEDYPIACIFGTPNIQPDHLKETVKGFYAGA"
BASE COUNT          387 a          366 c          318 g          633 t
ORIGIN      
        1 cagctcctgc ataaaatcct tttacagttt cttttaaatg gtcgggctga atgttgggag
       61 tgccaaaaat acaggcaatc gggtaatctt cgaatctgtt tgttcctctg gttccataga
      121 agtgaccaaa acattcagcg tctgggaagg ttaaattttt cttcgcttct tgccatcctc
      181 caaaaaaggc cttgtctttc aatagatcta ttatagcctg ctctttttga agaatgaccg
      241 ggtttgttgt tttttgccct tcttttgtag ggaagtggta ttttttccca tacctgccag
      301 aatagacctg aataactttt gaaccttctg gaagtgaaac gtttataatt ttctcttgca
      361 tcagggttcc ggtggcctgt tcccagatag atttatctgt cgtagcgtca agggcaataa
      421 tgccggcgtt ctttatcttt tctttgagtt ctcttttacg taagaagtaa aactcaatat
      481 ttttgtgttt ctcgtgatat ttcaggtata tgttactatt atagctatct ccgtgagttc
      541 tccagtcctt aatctctgcc tgtaatatac tgcagaaatc ctgcctccaa taatattcct
      601 tactctccca tatatcagag ggttttacac tcaggataaa atcaaggtct tttcccggcg
      661 gtgccaatct gctaaagtcg aattcaaatc tttttaggaa gtctgaacca tttaaaactt
      721 tgttttgtcc tgccatcttc aggccttccg ctatagcata aagggtttta tatatataga
      781 aatacggcgc cttcatatat tgcctttcca gttcgataac ggactgggta atctctattt
      841 cagggacttc aattttttca atgaaagagg atagcgggat ctcatctatt ataacccttg
      901 tgaccttctt tatattactg cctcccagca gcagcatctc atgggtcatg gccatgttct
      961 tgccggtatc ccattgtccc agatacatgc atgggctatt tttacagcga tctttatgct
     1021 cacatcctaa ctcacattta tagggtatat ggtgaaaacc tctcttttta cagagttcgt
     1081 ttatctcatc ggctttataa caatttccgg gttcctctcc ttccttatac tcttctttga
     1141 tctgctttct ccctcttatg atctgccatg catcggcggg aagttcccct ctgtcctgca
     1201 gctccttcag gtggtcaatc cgggcctgta caaggataaa aatattgtgt ctgttctttc
     1261 tatgcttttc tgtatagaag tttattgcta tagtgctttt gccgatgccg gggggacttt
     1321 ttataattac attcttttta tctatatctc tggtttttaa gttttcttca agagtattca
     1381 ggatctcttt tctggcttct tctattgtaa gcgcattgtt acagggtgtt tctgctttta
     1441 tctctcttat gcctttcttt ttattgtata gggggcagga ctgattgcag ggtaatttca
     1501 attctcttcc ataggcacaa tagaatttaa aattcatttt ataaatactc ttaatgtttg
     1561 gtttcagtct cttcctgcga tcctccggcg tcttatcata gtggcaggtg gtttgccatg
     1621 tggctatctc attaaaggcc tcttctatgc tcatgccggc tgcgtggcaa taggatgcat
     1681 gaaaattctc gtaataatgc atcc
//
LOCUS       DAOQWT010000392         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_85951_length_1703_cov_3.680825, whole genome shotgun sequence.
ACCESSION   DAOQWT010000392 DAOQWT010000000
VERSION     DAOQWT010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85951_length_1703_cov_3.680825"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PKJ95_07170"
     CDS             <1..538
                     /locus_tag="PKJ95_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNR66053.1"
                     /translation="SNILAASIVFFINDLPLPLIRNGIKTFSGIANRLEKIRTLRGIN
                     FYNDTCATTPDATIAAINSFSTEPLILILGGGDKKLDYRELCEVLKEKSNIIYTIILQ
                     HPAYNASEIILTQLKELGLSNKMEICSSMSGAIDVAYQKAIAGVNIILSPAATSFGMF
                     SNEFERGSVFRQAVDELV"
     gene            complement(555..917)
                     /locus_tag="PKJ95_07175"
     CDS             complement(555..917)
                     /locus_tag="PKJ95_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66054.1"
                     /translation="MVSIIFLLTIILNTLIGTIPFWGPLFSGIFTGIITEKKDLAVAV
                     AFWGAVIGGALCRIFLNYPRNFWHSYLLNIFGKTVAHYTKTIITGNLFFLALFFGLTG
                     ILGAFIGAFLKNQIKRRR"
     gene            complement(934..>1703)
                     /locus_tag="PKJ95_07180"
     CDS             complement(934..>1703)
                     /locus_tag="PKJ95_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HNR66055.1"
                     /translation="FLKKDSEFLYEKGDKPIVNILRNIFWFIFIISIPILLISSVVEL
                     ELNFLPLYEYAYQKYHISEVTGFTDEQLMRITRHLIQFFNGKVENVQLMMEKKGKPLY
                     LFHSHEIEHLNDVKVIFHYTFQVLIITLLCFAVYLIFTIARRDQNRWYHFWKALRGGN
                     ILTVGLLLVLGIFVFFGFYQLFLQFHYLVFGDPASTPWILDPRTDYLIMMYPLNFWQD
                     AAILGIGTIVIAALLFILISSIVLQGYRRKGRYHYKR"
BASE COUNT          579 a          330 c          299 g          495 t
ORIGIN      
        1 cagtaatatt ctggctgcct ctatcgtctt ttttatcaac gatttacctc tgcctctaat
       61 ccgaaatgga attaaaacct tttctggtat agcaaaccga ttagaaaaaa tcaggacttt
      121 aagaggaatt aatttttaca atgatacttg tgccacaacc cctgatgcta ccattgctgc
      181 cataaactca ttttctacag aaccattgat tttaattctg ggaggggggg acaaaaaatt
      241 agattaccgt gagctctgtg aagttctcaa agagaagtct aatattattt atacaattat
      301 tttgcagcat ccggcatata atgcttctga aataatcctg actcaactaa aagagctggg
      361 actaagtaat aaaatggaaa tctgttcttc tatgtccggg gcaatagatg tagcttacca
      421 gaaggcaata gcgggagtaa atataattct ttccccggct gcaaccagtt tcggtatgtt
      481 ttccaatgag tttgaaagag gcagcgtttt ccgccaggct gttgatgaat tagtttaagt
      541 caaatacttc ttatttatct tcttcttttt atttgattct ttaaaaaagc accgataaaa
      601 gcgcccaata tacctgtcaa gccaaaaaag agtgccagga aaaaaagatt tcctgtaata
      661 atggtcttag tatagtgagc aactgtttta ccaaatatat ttagcagata ggaatgccag
      721 aaatttcggg gataattaag aaaaatcctg catagggctc cccctattac cgctccccaa
      781 aaggcaacag ccacagccag gtcttttttt tcagtaataa tcccggtaaa aattcccgaa
      841 aaaagggggc cccaaaatgg gattgttcct attaaagtat ttaaaataat ggtaagaaga
      901 aaaataatgg agaccataag ctatccttta atcttatctt ttgtaatgat atctgccctt
      961 tcttcgatag ccctgcaata caatgctgct gattagaata aaaagcagtg ctgcaatgac
     1021 aatagtacct atccccagta ttgcagcatc ctgccagaaa ttcagcggat acatcataat
     1081 taagtagtct gttctggggt ctaatatcca gggggtgctg gcggggtcac caaaaaccag
     1141 gtagtgaaac tgtaaaaaaa gttggtaaaa accaaaaaac acaaatattc ccaataccaa
     1201 cagaagtccg accgttaata tattcccacc tctcaaggcc ttccagaaat gataccatct
     1261 attttggtct cgccttgcaa tagtaaaaat cagatagacc gcaaaacaaa gcagagtaat
     1321 tattaaaacc tgaaaggtat aatggaaaat taccttcaca tcattgaggt gttcaatttc
     1381 gtgactatga aataaatata agggcttacc cttcttttcc atcattaact gaacattttc
     1441 aactttaccg ttaaaaaact gaatcaggtg acgggtaatc ctcattaatt gctcatcagt
     1501 aaaaccggta acctcgctga tatggtattt ttgataagca tattcatata aaggcaggaa
     1561 attcagctct aactctacca ccgaggaaat caagagaatg gggatagaga ttataaatat
     1621 aaaccaaaat atattacgca gaatattcac tataggctta tctccctttt catatagaaa
     1681 ctcggagtct ttcttaagaa atg
//
LOCUS       DAOQWT010000393         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_86265_length_1699_cov_3.513382, whole genome shotgun sequence.
ACCESSION   DAOQWT010000393 DAOQWT010000000
VERSION     DAOQWT010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86265_length_1699_cov_3.513382"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..538
                     /locus_tag="PKJ95_07185"
     tRNA            452..538
                     /locus_tag="PKJ95_07185"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:486..488,aa:Leu,seq:cag)
     gene            complement(557..1015)
                     /locus_tag="PKJ95_07190"
     CDS             complement(557..1015)
                     /locus_tag="PKJ95_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66056.1"
                     /translation="MNNFEMRTKKKANIEILQQIRAEQKSIFALEHMLGYHISELEGL
                     RPHKRNIKFIAESNYNEQPDLVDLSHWLERVSNIEESIKKNEKIIIKVYDRLYAKIIL
                     KMIEGEELKPIDIFFYHSKKYIERIKYGEITFKKLVENYDWDAERFHNLD"
BASE COUNT          544 a          325 c          207 g          623 t
ORIGIN      
        1 catcaacagt ctttccatct ctacgcaagc ggaattctgc ttctttaata aaaaaagtct
       61 gtctaatttt atctcctaaa accaaatcaa caattctttt tctatttttc aaaacaatac
      121 ctccttccag ttgaaaggtt aaatcgtatt ttggctaact accaacgacc ggtgactaac
      181 aactactcag gcaatctcat tcacttataa cccgtattcc tggtctttaa caaactacta
      241 acgacaatcg actaacgaca atcaaggcaa tcacattcac ttattctcac taaaagttta
      301 acagggctac tgtaaataat ttagaatcag taattggtta tcagtgtatg ataattttct
      361 tgacaagcca ttcattttta tctatgctaa tatgtgtcag aaaaacaaat atgtttgaat
      421 gactctcttg ttttatctac aatatcttat cgccgaagtg gcggaatcgg cagacgcgct
      481 acgttcaggg cgtagtaggc ttaagcctgt gggggttcaa atcccccctt cggcaccata
      541 aattagattg gtattttcaa tctaaattat ggaatctctc agcatcccaa tcgtaatttt
      601 ccaccaattt tttaaaagta atttctccat attttatcct ttcaatatat tttttactat
      661 gataaaaaaa gatatctata ggttttaact cttctccttc tatcattttc aatattatct
      721 tggcgtaaag tctatcatat actttaatga taatcttttc attcttttta atactttctt
      781 ctatattact tacccgctct aaccagtgtg ataagtctac caggtcgggt tgttcattat
      841 aatttgattc ggcaataaat tttatatttc ttttatgagg cctaagcccc tctaattcgc
      901 tgatatggta tcccagcatg tgttccagcg caaagattga cttttgttct gctctgattt
      961 gttgtaatat ttcaatattg gctttctttt ttgttctcat ttcaaaatta ttcataatat
     1021 ttttcacata ctattatgtg taataaaaaa cctattatct gtgtatttat gccctatata
     1081 ccttaaaaat agtgcttggt aataggtaat aagtaaatag taagcttcga ttacaaaaaa
     1141 atttaatcta ccattattaa tcaaacatta aaaactataa actgaaaact ttatactgac
     1201 aacttatacc aacgacacac tatctatcga atgcaatggc aaccgtattt atttgttcct
     1261 acggaaatta tctgagagat aatctcctgt acaatctatt acttcaataa aaatgcaact
     1321 gagtagtcat tataacaaaa ttaatctaaa aagtgaaatg tcgttataga taggccaata
     1381 ccctcttttg ctaattataa acctattaac caattttaat aattttaata cctattttct
     1441 caatcctctg accactttaa tatttctcat atcatcccac tcactttttt tgggagtttc
     1501 taaaataaaa ggtaaagctt ttagcaatct atggtttact aatattttga aaccttctaa
     1561 tccaataaaa ccttctccga tatgggtatg cctatctttt cgggaaccta aaccatattt
     1621 ggaatcattg atatggatta tttttaatta actattagct catatgccac caggtctatg
     1681 ttactataat atcatagaa
//
LOCUS       DAOQWT010000394         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_86677_length_1694_cov_2.928615, whole genome shotgun sequence.
ACCESSION   DAOQWT010000394 DAOQWT010000000
VERSION     DAOQWT010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86677_length_1694_cov_2.928615"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PKJ95_07195"
     CDS             complement(<1..886)
                     /locus_tag="PKJ95_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR66057.1"
                     /translation="MVENKKNLSLKINSLAHAYNDAYLFVIPLLLPFFKLEFSFTYFQ
                     SGLILTLNEALRSVFSLLTGLFADRFGRKILIISLGFIISSLLLGSVLWVKKTSSIIT
                     VLLLMAIAVATFHPLATAMVGERAKPDKKGRDLSLFSAAGTFGLIVISLLFGWLVQTW
                     GWRLTCFIIAIPGLLIGLFYLKISEEKHEIEAIYSKANFGNLLTVFFLSRAVLCLGTK
                     VFLSFLPVYATTCIGLKPGISAWVISFYFLGVLVGSVFISNLLDQKKPLQFTIFSTAS
                     MVILIYFFTYSNLTLLTLI"
     gene            1200..1580
                     /locus_tag="PKJ95_07200"
     CDS             1200..1580
                     /locus_tag="PKJ95_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNR66058.1"
                     /translation="MTPQRINIYRCLIESKDHPSAEMVYHRIKREFPNISFDTVNRTL
                     HCLSEKGLIRKVESGYGPRRFDANLRTHYHFRCSNCKKIIDFDCSEYGNIKIPEGIAK
                     RFKVLRQEIILEGICPECLTKNTV"
BASE COUNT          638 a          263 c          292 g          501 t
ORIGIN      
        1 aaattagggt taacagtgtt aagttagaat aggtaaaaaa atagattaaa ataaccatag
       61 aagctgttga aaaaatagta aattgcaatg gtttcttctg gtcaagcaag ttactgataa
      121 aaacactacc gaccaggact cccaaaaaat agaaacttat aacccatgcc gaaattcccg
      181 gttttaatcc aatgcaagta gtggcataaa caggcaaaaa ggataagaac accttggtac
      241 ccagacataa tacagccctg cttaaaaaaa agactgttaa gagattacca aaattggctt
      301 tactgtaaat tgcttctatt tcatgttttt cttcacttat tttaagataa aacaagccga
      361 ttaataggcc gggtatggca ataataaaac aggttaatct ccatccccag gtctgtacca
      421 accatccgaa taacagactg atcacaatta gaccaaatgt tcccgcggcg gaaaaaagac
      481 tcaaatcccg tccttttttg tcaggtttag ccctttcgcc gaccatagca gtagccagag
      541 gatgaaaagt agcaacagcg attgccatca acagtaaaac agtaattatt gaggaagttt
      601 ttttaaccca tagcacactg cccaatagca gtgaagaaat gataaatcct agggaaataa
      661 ttagaatttt tcgaccaaaa cgatcggcaa ataatcccgt taataaagag aaaacagaac
      721 gtaatgcctc attaagggtt aaaatgagtc ctgattgaaa ataggtgaaa gaaaattcca
      781 atttaaaaaa aggtaataat aggggaatga caaaaagata agcatcattg taagcatgtg
      841 ctaatgaatt aatttttaaa gataaatttt ttttattttc caccattttc ctttactgat
      901 actgatttag aaattgttac tataattttt taaatcatta ctatttagca tactatttta
      961 gaagaatatt tcaagtattt ccataactat aattgacaaa taggaattga tactatatag
     1021 taattttgag caacataaga gagactttat tccataatct ttttataaac tcttataaaa
     1081 taaatattta aagaaaaaaa tgcagttcag taaatattta taataataaa ggaggcagga
     1141 ttaagctgga acagacatta tctttttgca aagaaaaact tgaagaacat ggttttagga
     1201 ttaccccgca gcgtataaat atttatcgtt gcttgataga atcaaaggac catccctctg
     1261 cggaaatggt atatcacagg ataaagaggg agtttcccaa tatatcattt gataccgtta
     1321 accgaacatt gcattgttta tcagaaaaag gcctgattag aaaggtggaa agcgggtatg
     1381 ggccaaggcg ttttgatgct aatctaagaa cacattacca ttttcgctgt tctaattgca
     1441 aaaaaattat tgattttgac tgctcggaat atggtaatat taaaattcca gaaggtattg
     1501 ctaaaagatt taaggtttta agacaggaga ttatactgga aggaatatgt ccggaatgtc
     1561 ttaccaaaaa tactgtttaa aataagttgc attggcaata gttactatat tttttcagga
     1621 aaaaattgta tcattataac ctgacttttg cagttcggtg ataaaaagat gccgttaaag
     1681 ccttttgtcg taag
//
LOCUS       DAOQWT010000395         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_87355_length_1686_cov_2.816677, whole genome shotgun sequence.
ACCESSION   DAOQWT010000395 DAOQWT010000000
VERSION     DAOQWT010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87355_length_1686_cov_2.816677"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..957
                     /locus_tag="PKJ95_07205"
     CDS             103..957
                     /locus_tag="PKJ95_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR66059.1"
                     /translation="MEYIKTIFIVGSINVVAVIGVSIFTGFTGLFSFGHAAFVAIGAY
                     GAAILTYFYHFPFILALIFGSFLAGLISLLVGYPTIKGNLGSDYLAVALLGFGEAVRV
                     ILENLKITQGARGLPGIRICCTVPVVIIAIIISVLIARNLIYSKYGRRMVEVREDAVA
                     AEMLGVPLFKTKYFSFFISAIFCGLSGGLFAHYYGFIQPAMFTLEQSTQILTSVVCGG
                     IGSITGPIVATYIFVFIPEVFRFAKLWRLVFYGAALVLTMIFRPSGLMGYKEIGFHSL
                     VAKIKRVE"
     gene            1023..>1686
                     /locus_tag="PKJ95_07210"
     CDS             1023..>1686
                     /locus_tag="PKJ95_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR66060.1"
                     /translation="MLPLLELQQVEKTFGGICALSDISFTVNKNEIIGLIGPNGAGKT
                     TIFNLVTGAYNISSGLILLNGKPINKKRPDQIVNLGVARTFQNIRLFKKLTVLNNVKA
                     SLYHKAKYNIFDALLKTGNYKKLEKDIDEEARILLKQMELDEYVEIQADNLAYGLQRK
                     LEITRALGLSPELLLLDEPAAGMNPKEVINIIDLIKKVHSEYRLTIIIIEHHMKVITS
                     LCS"
BASE COUNT          551 a          225 c          341 g          569 t
ORIGIN      
        1 cttgtgatta aaccattagg cctttttggt acgaagttag aagagaaaat ataaaaaata
       61 gttatttgag taaagaaaga ataataggga ggaaattttc cgttggaata tattaaaaca
      121 atttttatag taggttcgat aaatgtagtt gcagtaattg gagtaagtat ttttacagga
      181 tttactggtc ttttttcttt tggacatgct gcttttgtgg caataggggc atatggtgct
      241 gcaatattaa cctattttta tcattttcct tttattttag ctctcatatt tggcagtttt
      301 ctggcaggtt taattagttt gttagttggt tatcctacta ttaaaggaaa cctggggagt
      361 gattacttgg cggttgctct gttggggttc ggggaagcag tcagagtaat tttggaaaat
      421 ctaaaaataa ctcagggagc caggggtttg cccggcataa gaatttgttg caccgtaccg
      481 gttgtcatta tcgccataat catatctgta ttaattgcaa gaaatttaat ttattcaaaa
      541 tacggcagaa gaatggttga ggtcagagaa gacgctgttg cagctgaaat gcttggtgtc
      601 cctctattta aaactaaata tttttccttc tttataagtg caatattttg tgggttatca
      661 ggaggcttat ttgctcatta ctatggtttt attcaaccag ctatgtttac tttagaacaa
      721 tccactcaaa ttctaacttc tgtggtgtgt ggtggaatag gcagcatcac tggaccgatt
      781 gttgcaacct atatcttcgt ttttatacct gaagttttta ggtttgccaa attatggcga
      841 ttagtttttt atggtgccgc cttagtatta actatgatat tccggccaag tggattaatg
      901 ggatataaag aaattggttt tcatagttta gtagctaaaa ttaaaagggt ggaatagata
      961 atttaataaa gattgtctaa accgttaaaa taattgttga aattaaggga gatagtagca
     1021 aaatgttgcc tttattagaa ttgcagcaag ttgaaaagac ctttgggggt atctgtgctc
     1081 tcagtgatat ttcatttacc gttaataaga atgaaattat aggattgatt gggccaaatg
     1141 gggcagggaa aacaacaatt tttaatttag tgactggagc ttataatatt agtagtggcc
     1201 tgatcttatt aaatggtaaa ccaattaata aaaagagacc agatcagatt gttaaccttg
     1261 gtgtagccag gacatttcaa aatataagac tttttaaaaa attaacagtt cttaacaatg
     1321 tcaaagcatc actttaccat aaagctaaat ataatatatt tgatgctttg ttgaaaacag
     1381 gtaattataa aaaattagaa aaagatatag acgaagaagc caggatatta ttaaaacaaa
     1441 tggagttaga cgaatatgtt gaaatccagg cagacaattt ggcttatggg ttacagagaa
     1501 aattagaaat tacccgggca ttaggtttat ctcctgaact gctgctatta gatgagcctg
     1561 cagcaggtat gaatccaaaa gaggtaatca atataatcga tttaatcaaa aaagttcatt
     1621 ccgaataccg tttaaccatt ataattattg aacaccatat gaaagttatt accagccttt
     1681 gcagta
//
LOCUS       DAOQWT010000396         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_87555_length_1684_cov_2.671578, whole genome shotgun sequence.
ACCESSION   DAOQWT010000396 DAOQWT010000000
VERSION     DAOQWT010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87555_length_1684_cov_2.671578"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1114
                     /locus_tag="PKJ95_07215"
     CDS             164..1114
                     /locus_tag="PKJ95_07215"
                     /inference="COORDINATES: protein motif:HMM:NF025663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HNR66061.1"
                     /translation="MLNSKKIREKIIDSQYLFIIFTNLRAYIVYYLNRISNNELEKRR
                     FYKVLKYNVNLANPQSFNEKIFWKKIYDRNPLLPITADKYAVRAHIKKVLGEETANDI
                     LIPLLYITDKPEAIPFENLQIPYIIKPNHTSGRYIIIRNNDYNKRKIIITCKKWLKTP
                     FGLEKLEWAYQSIKRKIVIEKLLLDEEGNIPADFKFDMFDSRCKRIRVVRNRGSNQSN
                     LIYDSKWNLLLKIKGEQSPKFGIEKPKNFEIMIEIAEKLAADFDHVRVDLYNINGKIY
                     FGELTHYSQSGLINRISRNSDLELGKAWTIKPEYWKIDKT"
     gene            1197..>1684
                     /locus_tag="PKJ95_07220"
     CDS             1197..>1684
                     /locus_tag="PKJ95_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNR66062.1"
                     /translation="MLTSILQGMIPLLSFKMMSILFLGSLVGMLFGFIPGLDGMIGLS
                     ILIPLVYGMPLNISLALLLSMHASIAFGGSISAILFNIPGSGLNIATCFDGYAMSQKG
                     EAAKAIGIAGTSSFIGGFFGALVLAIFLPVIRPLILLFGPAEFLMVTIFGNLVCFLES
                     KK"
BASE COUNT          610 a          228 c          300 g          546 t
ORIGIN      
        1 ccaggatgca gattttataa tcgatgtgtt tatgcaagag atgtttgtca caatattgag
       61 ccagaattaa gagagattaa aaaaggctgg cttgttgcct gtcatattca gtaagatttc
      121 aagattagta tattgaaatt taagtagtga ggatttttaa atagtgttaa attctaagaa
      181 aataagagaa aagattattg atagtcagta tttgtttata atttttacaa atttaagagc
      241 atatattgta tattatttga atagaatatc aaataatgag ttagaaaaaa gaagatttta
      301 taaggtttta aagtataacg taaacctggc aaatccacaa tcatttaatg agaaaatatt
      361 ttggaaaaaa atttatgata ggaacccctt gttacctatt acggcagata aatatgcagt
      421 aagagctcac ataaaaaaag tattaggtga agaaacagca aatgatattt taatacccct
      481 tttatatata actgataaac cggaagctat accttttgag aatttacaaa taccttatat
      541 tatcaaacca aaccatacct caggtagata catcataatt agaaacaatg attacaacaa
      601 aagaaagatt ataataacct gtaaaaaatg gcttaaaaca ccctttggtc ttgaaaaact
      661 tgaatgggct tatcaatcaa ttaaaagaaa aattgtaatt gaaaagttac ttttagatga
      721 agaaggaaat atcccagcgg attttaaatt tgatatgttt gatagcagat gcaagagaat
      781 tcgtgttgtc cgtaataggg ggagtaatca gtctaacctt atttatgata gcaaatggaa
      841 cctattatta aaaataaaag gtgaacaatc tccaaaattt ggaattgaga aaccaaaaaa
      901 ttttgaaatt atgatagaaa ttgctgaaaa gcttgctgcc gattttgacc atgtccgtgt
      961 agatctttac aatataaatg gaaaaatcta ttttggtgaa cttacacatt attctcaaag
     1021 tggattaatt aatcgtatat caagaaactc tgatttagag ttaggcaaag cttggaccat
     1081 caaaccagaa tactggaaaa tagataaaac ctagttaaat atacataata aatagctatg
     1141 aaaccggaat aaaaataagt aactaaaata gataagaata attaaggagg aatcatttgc
     1201 taacttcaat attgcaaggg atgataccac tgctatcatt taaaatgatg tcaatattgt
     1261 ttttaggttc tctggttggc atgctctttg gtttcatacc tggacttgat ggaatgattg
     1321 gcttatccat tctaatccca ttagtatatg gaatgcctct taatattagt ttagccctgt
     1381 tactaagtat gcatgcctca attgcatttg gtggctcaat tagtgcaatt ctttttaata
     1441 tcccaggttc cggattaaat attgcaacct gttttgatgg gtatgcaatg tctcaaaaag
     1501 gggaggcagc caaagccatt ggtattgcag gcacctcatc atttatcggt ggttttttcg
     1561 gagctctggt tttagccata tttctacctg tcatcaggcc gcttattttg ctgtttgggc
     1621 cagcagagtt tttaatggtt acaatatttg gcaatttggt ttgctttctg gagagcaaga
     1681 aatg
//
LOCUS       DAOQWT010000397         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_87849_length_1681_cov_2.104551, whole genome shotgun sequence.
ACCESSION   DAOQWT010000397 DAOQWT010000000
VERSION     DAOQWT010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87849_length_1681_cov_2.104551"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..327
                     /locus_tag="PKJ95_07225"
     CDS             157..327
                     /locus_tag="PKJ95_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66063.1"
                     /translation="MLFFCNCVEHSCCFFICLANGNSAGTRSYSFMDRLCGDVPGKPA
                     SSGLCRTGASSL"
     gene            814..1026
                     /locus_tag="PKJ95_07230"
     CDS             814..1026
                     /locus_tag="PKJ95_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66064.1"
                     /translation="MLQIIEKEGDFMDLLINKEKEIKEIDNSTDNEEYWDKWDKEIEK
                     DSEEGKLDFLIKEAIDAKLKGYSKSL"
     gene            1027..1284
                     /locus_tag="PKJ95_07235"
     CDS             1027..1284
                     /locus_tag="PKJ95_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015219473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66065.1"
                     /translation="MHLTTPQFWIRFNNLPENVRNCSRKNVELLKENPYHPSLHFKKV
                     KGFWSVRVGLNYRALAIEEEGNFRWIWIGSHKEYEQIIKSL"
     gene            complement(1286..>1681)
                     /locus_tag="PKJ95_07240"
     CDS             complement(1286..>1681)
                     /locus_tag="PKJ95_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66066.1"
                     /translation="ILENEVKHLHAEKETLRQENEVLKEQVKALPDMKIIQEKEKDLM
                     IQIELERQEKEVLLSRAETIRKETEEKLKQSEEIHRKELEARQEEQKELHRQELEQVK
                     KEAEEEKKEIAETWRKELELSNRPWYKFW"
BASE COUNT          495 a          326 c          320 g          540 t
ORIGIN      
        1 agttgccgta cccccataat agggaacccc atccaagtat tgcaattttc atcaaacttc
       61 atcccattca ataaaaacaa aaaattatta tgataaaata aagttctata aaatatttaa
      121 gtataccttt aaatatttta acctgcagca ttttctttgt tatttttctg caactgtgta
      181 gagcatagct gctgcttttt tatctgcctg gctaatggaa attctgccgg taccagatcc
      241 tattcgttca tggatcgtct ctgtggagat gtgccgggaa aacctgcttc ctctgggtta
      301 tgccggaccg gtgcaagcag tctataacgg cctcggatca gggagaagtc tcgcgtcggc
      361 ttgcttgttc taactggaaa gctttatgca gcaagtcttt gagctactgc tcaaagattt
      421 gcgtcggctt tagatggaat ttgtaaaaga gagtaaccag cgagtcttct ccctgagctt
      481 agttggagtg agattgccgg gtttagcaaa tggagctgtt gggcatttat gcaatggccg
      541 gtagaaacag ttactaccag ttatgatggg tgtgtatggg gctttgtacc ggaaagcatt
      601 ttaccgctgt tttatgtatt catgcgggat ttctaattcc atatatatta tcaaatcagc
      661 gtagttacag gcgaaatcac tctaaaataa aaaaatgtca aaatatgaaa gcttgcccat
      721 ctggataatg gcgttcaagg tgattaagaa aataatctct tataccaata gccgggcctg
      781 ttcaagttcc ttaattgaca aaaacttttt aaaatgttac aaataataga gaaggaaggt
      841 gattttatgg atctgctcat aaataaagaa aaagagataa aagaaataga taatagcaca
      901 gataatgaag aatattggga taaatgggac aaagaaattg agaaagattc agaggaagga
      961 aaattagatt tcctgattaa agaggctatt gacgcaaaat taaaaggtta ttcaaaatct
     1021 ttataaatgc atttaacaac accacaattc tggatacgtt ttaacaacct tcctgagaat
     1081 gttcggaatt gttcaagaaa aaacgttgaa ctcttaaaag aaaatccata tcacccatca
     1141 ttacacttta aaaaagtaaa gggtttttgg tctgttagag taggtttaaa ttacagggcg
     1201 ttagctatcg aagaagaagg gaattttaga tggatatgga tcggaagtca taaagaatat
     1261 gaacagataa ttaaatcctt ataacctacc aaaatttata ccacggtctg ttactcagtt
     1321 ccagttcctt cctccaagtt tcagcaattt ctttcttttc ctcttcagcc tctttcttta
     1381 cctgctccaa ctcctgccgg tgaagctcct tctgttcctc ctgccgggcc tccagttctt
     1441 tcctgtggat ttcctctgac tgctttaatt tctcctccgt ctccttccgg atggtctcag
     1501 ccctggacag gagaacttct ttttcctgcc gttccagttc aatttgtatc atcaggtctt
     1561 tctccttctc ctgtattatt ttcatatctg gcaaggcttt tacctgttcc tttaaaactt
     1621 cgttctcctg tctgagagtt tccttttcag catggagatg tttcacttca ttttccagaa
     1681 t
//
LOCUS       DAOQWT010000398         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_88696_length_1671_cov_3.114480, whole genome shotgun sequence.
ACCESSION   DAOQWT010000398 DAOQWT010000000
VERSION     DAOQWT010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88696_length_1671_cov_3.114480"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..948
                     /locus_tag="PKJ95_07245"
     CDS             295..948
                     /locus_tag="PKJ95_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66067.1"
                     /translation="MKKSYILCISKLIWGLFLYALGSVIIINAHIGYAPWDVFHVGAA
                     KTIGVSVGTVSIVTGIFIGFLVFFLGEKLGLGTILNMVLIGGFIDIIFLINVIPLAKS
                     IIWSILMLIIGLIMLALGTYFYISSGLGAGPRDSLMVALTRKTGLPIGVCRGMIEVTV
                     VTIGWKLGGMVGVGTLVVAFTIGFWIQVTFKAFQFDATKIKHETLNQTYKGIFSINR"
     gene            complement(967..>1671)
                     /locus_tag="PKJ95_07250"
     CDS             complement(967..>1671)
                     /locus_tag="PKJ95_07250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR66068.1"
                     /translation="VLFLSRFYQEKGDSINYRIEDNKNNVRFWKSFSQLIKNRELVKI
                     SVFGFFLGMMSSSITSHFTLFLFLDYGLPETIAGLGFAFVQMGSILGRVAWGIFCDKV
                     MGSDKRKTFLITGILFWLTTIILSICSRNINPSISMLFLLAFLAGCFGNGWPGIFCAS
                     IAEVVEEKNIGIATGAAFLLVRSGLMIAPPIFGYISDMKGSYSLSWFLLGIIMLITSL
                     GQYLFSKKRDGNRLKI"
BASE COUNT          550 a          295 c          285 g          541 t
ORIGIN      
        1 cattttatac tataagagcc actattggca tctctggaga taactttatc aaaattataa
       61 tgcctcttca tataccattc cccttcccca ctctttttta tatttacagt gtactaataa
      121 aagttttgag ggtcaagcta ttgacagcta tgtagtaagt ggaggaaatt gttgacttta
      181 ttattgatga ttctataatg ggtatcatgc atacttattt ggctgaaata tcaaaagaaa
      241 aaaatttaat attacttact ctttatttaa aaaccaataa aagaaagccg tattgtgaaa
      301 aaatcgtata tattatgtat ctctaaatta atctgggggc tttttcttta cgcattgggc
      361 agtgttatta tcataaatgc acatataggg tatgccccct gggatgtctt ccatgtagga
      421 gcagctaaga caataggtgt cagcgttggt accgtatcta ttgtaaccgg tatttttatt
      481 ggctttttag tcttcttttt aggagaaaag cttggtcttg gcacaatcct taatatggta
      541 ttaatcggtg gttttataga tattattttt ttaattaatg tcataccact ggctaaaagt
      601 atcatctgga gtattcttat gctgattata ggattaataa tgcttgccct gggaacctac
      661 ttctatataa gctctggatt gggtgcagga ccccgtgata gtctgatggt agcacttacc
      721 aggaaaacag gcttacccat tggtgtctgt cggggtatga ttgaagttac agtagtaact
      781 attggctgga aattaggtgg catggtcgga gtgggaacct tagtagttgc ctttaccata
      841 ggattttgga tacaggttac ttttaaagca tttcaatttg atgctaccaa aataaaacac
      901 gaaacactca atcagaccta taaaggcatt ttttcaatta atagataaaa tgaggtacct
      961 tcaatctcag atttttagcc ggtttccatc ccttttttta gaaaataaat actgaccaag
     1021 agaagtaata agcataataa ttcccagtaa aaaccagctt aaactataag aacccttcat
     1081 atctgaaata tagccgaaga tgggtggtgc aatcatcaat cctgatctta ctaaaagaaa
     1141 agcagctccg gtagcaatgc ctatattttt ttcttccact acttcagcta tagatgcaca
     1201 aaaaatacca ggccaaccgt tcccaaaaca accagccaga aaagccaata aaaagagcat
     1261 gctaatagat ggatttatat ttcttgaaca aatacttaag atgattgtgg ttaaccagaa
     1321 taatattcct gtaattaaga aagtttttct tttatcagac cccattacct tatcacagaa
     1381 tattccccag gcaacccttc ccaaaatgct gcccatctgt acaaatgcaa aacctaaacc
     1441 ggctatggtt tcaggtaaac cgtaatccag gaataaaaaa agggtgaaat gggaggtaat
     1501 tgaactgctc atcattccta aaaagaatcc aaaaactgaa atttttacta actctctatt
     1561 tttaattaat tgtgagaaag acttccaaaa acggacatta tttttgttat cctctattct
     1621 ataattaatt gaatctcctt tttcctgata gaagcgggat agaaataaaa c
//
LOCUS       DAOQWT010000399         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_88768_length_1670_cov_3.550464, whole genome shotgun sequence.
ACCESSION   DAOQWT010000399 DAOQWT010000000
VERSION     DAOQWT010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88768_length_1670_cov_3.550464"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PKJ95_07255"
     CDS             complement(<1..1115)
                     /locus_tag="PKJ95_07255"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR66069.1"
                     /translation="MNNEFENFDAIIVGSGQGGTPLAMELARSSWKVALVERWHVGGS
                     CINIACTPTKTMIASARVAHLVSRAGEFGINSNIELVDLLKIVERKKEVVESFRNGLK
                     KQLLSTKGLTLMEGEARFIGKKLLEVSMKNGEKSSIKSDLIVINTGASPSVPPIKGLE
                     TTKYYNSTSIMEIKELPRHLLVIGGGYIGLEFGQMFRRLGSKVTIIQLDGQLLPNEDS
                     DIAEEVTSILKGEGIEILLSAKTEQVQTSQNGEIVLTFIKDKKEQTVEGSHLLVATGR
                     VPNSSNLNLETTGIKTEQRGFIPVNNRLETEVPGIFAIGDVNGEPQFTHISYDDYRII
                     SKNILQNGNATTSGRQLPYVVFIDPQLGRIGMTEKAA"
     gene            1327..>1670
                     /locus_tag="PKJ95_07260"
     CDS             1327..>1670
                     /locus_tag="PKJ95_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNR66070.1"
                     /translation="MKRFFIFLTILFISLNLVACNNEQGKEEAKKGPVTIGTFIDSEG
                     AVLGKMMVMLLEQEGYEVIDKTEFGTPDILRKALLAGEVNLVLDYTGSGQYYHDVEDI
                     DTDIWSDAREGY"
BASE COUNT          472 a          345 c          293 g          560 t
ORIGIN      
        1 cttgccgctt tttcggtcat accaatacgg cccagctgtg ggtctataaa aactacatag
       61 ggcaattgtc ttcccgaggt agtggcatta ccattttgta aaatattttt acttataatc
      121 ctgtagtcat cataagagat gtgagtaaac tgcggttctc cattaacatc tcctatggca
      181 aatatacccg gaacttccgt ctccaaacga ttattgaccg gaataaaacc acgttgttct
      241 gttttgattc cggtagtttc aaggtttaaa ttagaactat tgggaaccct tcctgtggca
      301 accaatagat ggctgccctc aacagtctgc tctttcttat ctttgatgaa ggttaataca
      361 atctctccat tttgggatgt ttggacctgc tctgttttgg cactaagtaa tatttcaatc
      421 ccttccccct tcaggataga tgttacttct tcggctatat ctgaatcctc attgggaagc
      481 agttgtccat ccaattgtat gatggtcact ttactgccca atcttctaaa catctgccca
      541 aattccagac caatataccc tccacctatt accaagagat gccggggtaa ttcttttatt
      601 tccattatcg atgttgaatt ataatatttt gttgtttcca aaccctttat tgggggtaca
      661 gaaggactgg caccggtatt aattactatt aaatcggact taattgagga tttttctcca
      721 tttttcatac ttacttctaa aagtttttta ccgataaaac gtgcttcgcc ttccatcaag
      781 gttagtcctt ttgtagataa caattgcttt ttaagcccat ttctgaagct ctctactact
      841 tccttcttgc gttctacaat ttttaacaaa tcaaccaatt ctatattaga gttaatgcca
      901 aactccccag ccctgctcac caggtgagca actctggctg aggcaatcat agttttggta
      961 ggggtacagg caatattaat acaggaaccg cctacatgcc agcgctctac cagtgcaacc
     1021 ttccaactag atctggccag ttccatagcc agtggggttc ccccctgacc tgaaccaaca
     1081 attattgcat caaaattttc aaattcatta ttcattttta aatcctcctt actgaaatac
     1141 tgattaatac catatcagac aaactaatat aggttgtgaa cacgtcttat attataccat
     1201 tattcatacc ctacagtatc ttttctaaac ttctatatat ttttgtgaac ctttattcaa
     1261 gcccttgctt tcattataag acattttttg ttatactctt tttaaaagaa caaagggggt
     1321 ttatttatga aaaggttttt tatttttctg accatacttt ttatatcttt aaatttagta
     1381 gcctgtaata atgaacaggg taaggaggag gctaaaaaag gaccggtaac tattggtact
     1441 tttattgaca gcgaaggtgc cgttctggga aagatgatgg taatgttatt ggagcaggaa
     1501 ggctatgaag tcattgataa aacagaattt ggaacaccag atatattaag aaaggcactg
     1561 ctggcaggcg aagtcaattt agtgctcgat tataccggtt ccggtcagta ttaccatgat
     1621 gttgaagaca ttgatactga catatggtca gatgccagag agggatatat
//
LOCUS       DAOQWT010000400         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_89721_length_1659_cov_2.462594, whole genome shotgun sequence.
ACCESSION   DAOQWT010000400 DAOQWT010000000
VERSION     DAOQWT010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89721_length_1659_cov_2.462594"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PKJ95_07265"
     CDS             complement(<1..191)
                     /locus_tag="PKJ95_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006993870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNR66071.1"
                     /translation="MMDVIKRVKKIIVDQLGIEEEKVKEESSFIDDLGADSLDIVELI
                     MAFEEEFDIEIADEDAEKI"
     gene            complement(221..805)
                     /gene="fapR"
                     /locus_tag="PKJ95_07270"
     CDS             complement(221..805)
                     /gene="fapR"
                     /locus_tag="PKJ95_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HNR66072.1"
                     /translation="MEKRLNKEKRQEKIVERISVNPFLSDEQLAKIFRVSIQTIRLDR
                     LEMGIPELRKRVIKLAQDASSKVKALNKNEIIGDLIDIDLGKSGISTMHTTKEMAFGK
                     NSVVRSHYIFSLADSLAMSIIDSDVVLTGVARLRYRNPVYAGQLLVAKARIASKRSNK
                     YLVSVHIKCNHKEVFTAKLIMVSIEQRSNNISFN"
     gene            complement(995..1195)
                     /gene="rpmF"
                     /locus_tag="PKJ95_07275"
     CDS             complement(995..1195)
                     /gene="rpmF"
                     /locus_tag="PKJ95_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNR66073.1"
                     /translation="MANPKRKTSKSRKNKRRTHWIEKTINLVECSRCHEFKLPHHVCP
                     SCGYYKGEKILPEKKASEAKTT"
     gene            complement(1300..>1659)
                     /locus_tag="PKJ95_07280"
     CDS             complement(1300..>1659)
                     /locus_tag="PKJ95_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNR66074.1"
                     /translation="GAKHPLVDKVVKATEIAKRKSPDLNIDGELQADAALVPEIGKRK
                     APNSRIAGEANVLIFPDLQAANIAYKLVERLAKAEAIGPISQGMRKPVNDLSRGCSVK
                     DIVNVVAITALQAQGIQ"
BASE COUNT          496 a          314 c          220 g          629 t
ORIGIN      
        1 ttaatttttt cagcatcttc atcagcgatt tcaatatcaa attcttcttc aaaggccatg
       61 atcaattcta caatatccaa agaatcagca cccaagtcat caataaatga agattcttct
      121 tttacttttt cttcttcaat cccaagctga tcaacgataa tttttttcac tctttttatt
      181 acatccataa tcattcaatc tccttaatat tttcattatc ctaattaaaa gaaatgttat
      241 tagatctttg ttctattgaa accattatta gtttagcagt aaaaacttct ttatggttac
      301 attttatatg gaccgacact aaatatttat tacttctttt tgaggcaatt ctagccttgg
      361 caactaaaag ttggccggca tataccgggt ttctatacct taatcgagct acaccggtta
      421 ataccacatc agaatcaata attgacatag ccagtgagtc agcaagggaa aatatataat
      481 gacttctaac cacactattt ttaccaaaag ccatctcttt agtagtatgc atagtcgaaa
      541 tgccactttt tcctaaatcg atgtctatca gatcaccaat tatttcattt ttgtttaatg
      601 cttttacttt tgaggacgca tcctgggcaa gcttaattac tcttttacgc aactcaggta
      661 tgcccatttc taatctgtca agtctaattg tttgaatact aacccggaat atttttgcta
      721 attgttcatc tgataaaaaa gggttaacag aaattctttc aactattttt tcctgtcttt
      781 tttctttatt taatcgtttt tccatcattt gtttatggca aatatcaata tttattttat
      841 tagcaactat taaaggcagc ccctattact gactcatagt atagacaaat atttttataa
      901 aatcaagacc aatattatat ttttaatact aaattattaa aatgaatgtt atttaataaa
      961 aaaataaaca aatacctatt tttaagttaa agaattaggt agttttagct tccgaggctt
     1021 tcttctcagg aagaattttt tctcctttgt aataaccaca ggaaggacag acatgatggg
     1081 gtagcttaaa ttcatgacat ctagaacatt ctacaagatt aatcgttttt tctatccagt
     1141 gtgttcttct tttatttttt ctcgatttag aggtttttct ttttggattt gccacttttt
     1201 gtctcctttt caggtcaata attcttattt ataccaacaa aaaactatta tatataaaat
     1261 ttcaatcgtt tgcaaattta ttatagataa ctttacatac tattgaatgc cttgtgcctg
     1321 aagagctgta attgccacaa catttacaat gtccttaact gaacagcctc tggagagatc
     1381 atttaccggc tttcgcatac cctgtgagat gggccctatt gcctctgctt ttgctaatcg
     1441 ttctactaat ttataggcaa tattagcagc ctgtaaatct gggaatatta atacattagc
     1501 ctctccggca attctgctat tgggagcctt tcttttccct atttcaggaa ccagggcagc
     1561 atctgcctgc aattctccat caatatttaa atcaggagat tttcttttgg caatttcggt
     1621 tgcttttacc actttgtcga ccaagggatg tttagcacc
//
LOCUS       DAOQWT010000401         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_90036_length_1655_cov_2.843750, whole genome shotgun sequence.
ACCESSION   DAOQWT010000401 DAOQWT010000000
VERSION     DAOQWT010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90036_length_1655_cov_2.843750"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PKJ95_07285"
     CDS             <1..603
                     /locus_tag="PKJ95_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNR66075.1"
                     /translation="CYQCGKPFSPEVDFEAKALCADCLYRGHHYYFVRSLAYYEGVLR
                     KCIHLLKFKKQVKLVKPLANLMINYLSRDELINIKEIELIIPVPLFKDDHLKRGFNQS
                     GLLAKYIADYFSIPFSEDLLIKIRANLSQVGLSRIERKKNVKNVYAINSSMLQKQHNI
                     SSLLLIDDIFTTGASIEACCKELKKIGIEKLYVFTLARGV"
     gene            975..1523
                     /gene="raiA"
                     /locus_tag="PKJ95_07290"
     CDS             975..1523
                     /gene="raiA"
                     /locus_tag="PKJ95_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNR66076.1"
                     /translation="MNLIVKGKQIEVTDSVDNYIRKKMNKLEKYYDQIIEAVATISME
                     KNRHKFEVTLQAKKSIIRAEEESDSIFNSIDRVVERLERQIIKYKEKLYSKYGNEYNK
                     EKAGEIPAGEEIIVENPTDQNRKIVRIKRFVIKPMSPEEAGLQMELLGHSFYVFSNEE
                     TAQLNVIYKRKDGDFGLIEPEV"
BASE COUNT          627 a          204 c          338 g          486 t
ORIGIN      
        1 tgttatcaat gcgggaaacc tttttctccc gaagtagatt ttgaggcaaa ggccctgtgt
       61 gctgattgtc tttatagagg acaccattat tattttgtca gatcacttgc ctattatgag
      121 ggagtattaa gaaaatgtat acatttactt aaatttaaaa aacaggtaaa attagttaaa
      181 ccattagcta atctaatgat taattatctc tcaagagatg aactgattaa cataaaagag
      241 attgaattaa taataccggt acctttgttt aaggatgatc atttaaaaag aggttttaac
      301 cagagtggtt tattggcaaa atatattgct gattattttt ccattccctt ttctgaagat
      361 ttattaataa aaataagagc taatttgtcc caggtgggtc tgtccagaat agagaggaaa
      421 aagaatgtga aaaatgtcta tgccattaat tcatctatgc tgcaaaaaca gcataatatt
      481 tcaagccttt tattaataga tgatatattt accaccggtg ccagcattga ggcctgttgt
      541 aaagaattaa aaaaaattgg aatagaaaag ctgtatgttt ttacattagc cagaggagtg
      601 tgaaggtcaa taatactccc tccttagagc tgtggttgaa agtcgacgcc agccgcaggc
      661 aaaacgatcc acgtaaggta acgattatca gttgctgagc atggtgcggt tttggagtaa
      721 gtcctgcctt attattaaaa aaagataaga gaagagtagt agtgaaaaga tagcgaacct
      781 ctcagcaggc gaatgtgggg gaaaaaacca ggtcagtcta gggagggagt aaacattcca
      841 aaatgaaaat gagtaattac atcttgagag taatacagtt aaaataatta attattgtta
      901 aataagtagg aaaaaacgca ggtaaatgtt ataatataat gatataggtc tacttaactt
      961 aaggaggaaa ataaatgaat ttaatcgtca aaggaaaaca aattgaagta actgattctg
     1021 ttgataatta tataagaaag aaaatgaata aattagaaaa atattatgat cagattattg
     1081 aggctgtggc cactattagt atggagaaaa acaggcataa atttgaagta actctacagg
     1141 ctaaaaaatc tataatcagg gctgaagaag aaagtgatag tatttttaac tccattgaca
     1201 gggtagtgga aaggttggaa aggcagatta ttaagtataa agaaaaacta tacagcaagt
     1261 atggtaatga gtacaataaa gaaaaggcgg gtgaaatacc ggcaggagaa gaaattattg
     1321 ttgaaaatcc aaccgaccag aatagaaaaa ttgttagaat aaaaagattt gttataaaac
     1381 caatgtctcc agaagaagct ggtttgcaga tggagttatt ggggcatagt ttttatgttt
     1441 tcagtaatga agagactgct caattaaatg tcatttacaa aagaaaagat ggcgattttg
     1501 gcttgataga acctgaagtt taaaagttaa aaagggagag cctgatgatg aataatatcc
     1561 ctgttaatga ttttcgtaat tttattattt caccccagtt tgcctcaaaa gtaaaggata
     1621 ttgaaaaatc aataaattct ttgaatggtg tattg
//
LOCUS       DAOQWT010000402         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_90763_length_1647_cov_2.991834, whole genome shotgun sequence.
ACCESSION   DAOQWT010000402 DAOQWT010000000
VERSION     DAOQWT010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90763_length_1647_cov_2.991834"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKJ95_07295"
     CDS             complement(<1..623)
                     /locus_tag="PKJ95_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66077.1"
                     /translation="MTSEKMQNQNTFFLKITEESDLSLEVVILVILGVFMLLFGFLLF
                     QIHRGALPYTPDSTYGLFLVIVSLQIITMGKTPFGDLRRSWILITVGIGTAIIGMTAG
                     FIPGYLSELVRLLVGFMLLAGGIALILQLFFSRDKAKTWIKASGMLQHLTIACTIVYA
                     LTIVEGVITLQPDIIITPQIAVITIIYGLSFFYLSWCLHKVSHIYPS"
     gene            complement(747..1616)
                     /locus_tag="PKJ95_07300"
     CDS             complement(747..1616)
                     /locus_tag="PKJ95_07300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR66078.1"
                     /translation="MSSSDYYKDKICIVTGGNSGIGYALCEELLIRGAIVYMAGRNPQ
                     KVSDAAKKLSEYGDRIHTVIVDVTVQEQVQRAIEDIAAKEGRLDMLFNNAGVGGTLPF
                     TFATLEDWKNIIDTNIWSVIYGVYTAVPIMLKQGSGHIINTSSIAGILPPPFQSLYCL
                     TKFGVTGLTESLKYEYADRGLNFSTICPGFVASAIYNKGVDGKPKGELKIPADAYPAD
                     KAAVDILDGVQKQEGIICVPNELKENWEDYALGKGDESLLEIARVRREEYKKILPEMD
                     LGSEEYEEKLMLL"
BASE COUNT          498 a          352 c          291 g          506 t
ORIGIN      
        1 tttgaaggat aaatgtggct aactttatga agacaccagg atagatagaa aaagcttaat
       61 ccatagataa tggtaattac tgctatctgt ggagtgatga ttatgtcagg ttgtagtgtt
      121 atcacgccct caacaattgt cagggcataa actatggtac aagcaattgt caagtgttgt
      181 aacatgccag aagcctttat ccatgttttt gccttgtctc tggagaaaaa tagctgcaga
      241 ataagagcaa ttccacctgc aagaagcata aatcccacca gtaatcggac aagttcagaa
      301 aggtatcctg gaataaaacc tgctgtcata ccaatgatgg cggtgcctat tcctacagta
      361 ataagtatcc aggaacgacg gagatcacca aagggggttt tgcccatggt gattatctgg
      421 agagaaacaa taacaaggaa aagtccatat gtgctgtctg gagtgtaagg taaagctccc
      481 ctatgaattt gaaaaagaag gaagccgaat agaagcatga acactcctaa gattactaaa
      541 atgacaactt ctaatgaaag atctgattcc tcggtgattt ttaaaaaaaa tgtgttttga
      601 ttttgcatct tttcggatgt cataaaacat cccccttaat atggcaccac gaactcatgg
      661 tcacccaaat ctgtttcatc ttccaatctc tgaatctaca atattacact tcgctttggt
      721 atctcgatat tatggtactt tcaaatttac aacagcatca gcttctcttc atattcttca
      781 ctaccaagat ccatttccgg gagtattttt ttatattcct ctctgcgcac ccttgctatt
      841 tccaataaac tttcatcccc tttcccaagg gcataatctt cccaattttc ttttaactca
      901 tttgggacac agatgatgcc ttcttgtttt tgaactccat ccaaaatatc tacggctgct
      961 ttatcagcag gataggcatc agctggtatt tttaattctc ctttaggttt gccatcaact
     1021 cccttattgt aaatggctga ggccacaaaa cctgggcaga tagttgagaa attaagaccc
     1081 ctatctgcat attcatattt taatgactca gtaagtccgg ttacaccaaa tttggttaga
     1141 cagtagagtg actggaaggg aggggggaga ataccggcaa tggaactggt gttaattata
     1201 tgtccagatc cctgttttaa catgatgggt acagcggtgt aaacaccgta aataacactc
     1261 caaatatttg tatcaattat atttttccaa tcctccaggg tagcaaatgt gaatggaaga
     1321 gtaccaccaa cacctgcatt attaaataac atatctaatc taccctcctt tgctgcgata
     1381 tcttcaatgg ctctctgcac ctgttcctga actgtcacat ctactatgac tgtatgaatc
     1441 ctatcaccat attcagaaag cttttttgca gcatctgaaa ctttttgagg gttacgccct
     1501 gccatgtaaa cgattgcccc tcttatgagg agttcttcac ataaagcata cccaataccg
     1561 gaatttccac cggtaacaat acatatttta tctttataat aatctgaact gctcataata
     1621 atcaccccta cctcttttta ttcgatg
//
LOCUS       DAOQWT010000403         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_90775_length_1647_cov_2.775754, whole genome shotgun sequence.
ACCESSION   DAOQWT010000403 DAOQWT010000000
VERSION     DAOQWT010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90775_length_1647_cov_2.775754"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="PKJ95_07305"
     CDS             <1..1276
                     /locus_tag="PKJ95_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNR66079.1"
                     /translation="KETEVIDALRTLSMLSGINIVADDSVIGQITLSLNEVSFKDALN
                     WILSLKRLKYTQVGNALIVGTEDIIKTYRERVTKIVHLENADVENTKSVLDHYFDDKE
                     NIKVTTDTRLNNLILEGLPELVSKSEELIKQMDTSLITKTFKLDNAIFGEEVEAIEKM
                     LGIIIPDESRVLVDSRQNEIIIKGNQEEIANARIMIDGLDKRVPQIMIESKIIEIELN
                     AKKDLGISWSAGSTTDEQGNIILGQITFGEINLGGSFERRDLIQARLDALTTEGKTNV
                     LSNPKILTLDGKESVILSGSKIPIRERTAEGIETVRYIDVGLNMTITPRLSQDDKITM
                     DCDIKIESLGTELIQGYPIINSRQEKAIIRSPLGITNVIGGLISSEEIETIRRIPILS
                     EIPVLGELFKFTNRTKKRTEIIILITAHRIEY"
BASE COUNT          572 a          249 c          334 g          492 t
ORIGIN      
        1 gaaagaaaca gaggttattg atgctcttcg gactctatca atgttaagtg gaataaatat
       61 tgtggcagat gactcagtta tcggacaaat tactcttagt ttaaatgagg tttcttttaa
      121 agatgcactc aattggattc tgtctttaaa gagattgaaa tatactcagg taggaaatgc
      181 tctaattgtt ggaactgaag acataattaa aacctatcgg gaaagggtta ccaaaatcgt
      241 tcatttagaa aatgctgatg tggaaaatac taaaagtgtt ttagaccatt actttgatga
      301 taaagaaaat ataaaggtaa ccacagatac cagattaaac aatctcatac tggaagggtt
      361 gccggaattg gtttccaaat ctgaagaatt aattaaacag atggatacca gtttgattac
      421 taaaaccttc aaactggata atgccatttt cggagaagag gtagaagcca ttgagaaaat
      481 gcttggtatt ataattccag atgagagtag ggttttggta gatagccgtc agaatgaaat
      541 tattattaaa ggaaatcagg aagagatagc caatgccaga ataatgattg atggattaga
      601 caaacgtgtt ccccagataa tgattgaatc aaaaatcatt gagattgagc ttaatgccaa
      661 aaaagacctg ggaatttcat ggagcgcagg ctcaacaacc gatgaacagg gaaatattat
      721 attaggacaa ataacttttg gtgaaattaa cctgggcggt tcttttgaga ggagagacct
      781 tattcaggca aggttggatg ctctgaccac agaagggaaa accaatgttt tatcaaatcc
      841 gaagatatta actctggatg gtaaagaatc agttatactt tctggttcca agattcctat
      901 tcgggaaaga acagcagaag gaattgaaac tgtccgttat attgatgtcg gtctgaatat
      961 gactatcacc ccacgcttga gtcaggatga taaaatcact atggattgtg atattaaaat
     1021 cgaatctctg ggcactgaat taattcaggg atatccaatc attaattctc ggcaggaaaa
     1081 agcgattatc cgttcacctc ttggtataac aaatgtaatt ggcggattaa tatcttctga
     1141 agagattgaa acgattagaa gaattcctat tttaagtgaa ataccagtat taggtgaatt
     1201 atttaaattt accaatcgta ctaagaagag aactgaaatt ataatcctta ttactgcaca
     1261 caggattgaa tattagaaat aagaagtaat tacatagtgg tggcatagtg agtgaaattt
     1321 tctatgatgt taaaatttgt accttctaaa ttacaatatt gctgaagaga gacacaaagc
     1381 atttgtattt atgccaggat aagtgaatga gattgccatg acgtactttc agtatgtctc
     1441 gcaatgacta aaaataggtg tcattataaa gagcatcttt caccaaagaa tagcaatgag
     1501 ttatgctgcc ctcgtcattg tggggaaaat gcccctttgt aattgcaagt gtttcccctc
     1561 tcgtcattgc gagtaatttt ctcctctgtc attgcgaaca ctatgacctt gtcaagtact
     1621 ttttacgtac tttttacata catagga
//
LOCUS       DAOQWT010000404         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_91693_length_1637_cov_3.289507, whole genome shotgun sequence.
ACCESSION   DAOQWT010000404 DAOQWT010000000
VERSION     DAOQWT010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91693_length_1637_cov_3.289507"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="PKJ95_07310"
     CDS             <1..1135
                     /locus_tag="PKJ95_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR66080.1"
                     /translation="GLPVGHMQFIEIAREIDKTGLKLLVFDEPTAVLTESEADNLIST
                     MKRLAESGIAILFITHRLSEVMQVADNITVLRDGELAATKKREETNVAELAEIMVGRK
                     IDLKSKTEQEKECKEKEAILKISNLQVDMPGEKVSRVNLEICKGEIFGIGGLAGQGKV
                     GIANGVMGLYPTKGEIIFKGEKLELNNTKNSLNKGLAFVSEDRRGVGLLLDSSIELNI
                     VITTMQTKDKFLKRFGFINQLDSNEIREYANRAIKELDIRCTKPQQLTRQLSGGNQQK
                     VCVARALALEPEVLFVSEPTRGIDVGAKKLVLDLLLKLNKELGMTIIMTSSELVELRS
                     ICDRIAIIFRGEVAGILKASNSDREFGLMMAGEYQKFSSEEAG"
     gene            1137..>1637
                     /locus_tag="PKJ95_07315"
     CDS             1137..>1637
                     /locus_tag="PKJ95_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR66081.1"
                     /translation="MNDTLSKIINRIGLPRLIISLLFLAICITAVVIKLPFGMIVSDI
                     IRRFGMFGVLSLAMVPAIECGTGPNFALPIGIICGLLGSLIAIEFSLTSPLLGYGFIS
                     SILISLPLAILSGWLYGIMLNKIKGSEMLVATYTGFSIVSFLQIGWVTLPFTHPEMRW
                     PIGKGLR"
BASE COUNT          510 a          260 c          400 g          467 t
ORIGIN      
        1 cggactaccg gtaggacata tgcagtttat agagatagcc cgggaaattg ataagacagg
       61 cttgaagctc ctggtatttg atgaacccac tgcggtgctc actgagagtg aagcagacaa
      121 tcttatttct actatgaaga gattagccga atcaggaata gccattcttt ttatcacaca
      181 cagactaagt gaagtaatgc aggtagcaga taacattact gttttaaggg atggtgaact
      241 ggcagcaact aaaaaaagag aagaaacaaa tgttgcagaa ctggctgaaa taatggtggg
      301 gcgaaaaatt gacctgaaga gtaaaactga gcaggaaaag gaatgcaaag aaaaagaagc
      361 gattttgaaa atcagcaatt tacaggtaga tatgcccgga gaaaaggtga gtagggtgaa
      421 cctggaaatt tgtaaaggtg aaatatttgg aattggagga cttgccggtc agggaaaggt
      481 ggggattgcc aatggggtta tgggtttgta ccctactaaa ggcgaaataa ttttcaaggg
      541 agaaaagctg gaactgaata acaccaaaaa ttctttgaat aagggactgg cttttgtttc
      601 tgaagacaga aggggagttg ggttattgtt agattcctcc attgagttaa acatagtaat
      661 caccactatg cagaccaaag acaaattttt aaagagattc ggatttataa accagctgga
      721 tagtaatgaa atcagagaat atgccaatag agctatcaaa gaattagata taagatgtac
      781 caagcctcag cagttgacca gacagcttag cggtggtaat caacagaagg tttgtgtagc
      841 ccgggctttg gctttagaac ctgaagtatt gtttgtttcc gagccaaccc gcggaattga
      901 tgttggtgcc aaaaaactgg ttcttgattt attgttaaaa ctgaataagg agctgggtat
      961 gaccattatt atgacttcca gcgagctggt agagcttcgt tctatctgtg atagaattgc
     1021 cattatattt agaggtgaag tagccgggat ccttaaggca agcaatagtg accgggaatt
     1081 tggcttaatg atggcgggag aatatcaaaa attttccagt gaggaggcag ggtaaaatga
     1141 atgatacatt gagtaaaatt attaacagga taggtcttcc cagacttatt atcagccttc
     1201 tctttctcgc tatttgtata acagcagtgg tcattaaact cccctttgga atgatagtgt
     1261 ctgatatcat cagaaggttt ggtatgtttg gggtgctctc actggctatg gtacctgcta
     1321 tagaatgcgg aaccgggccg aattttgcct taccaattgg aattatttgc ggattactgg
     1381 ggagtcttat tgctatagaa tttagtttaa cctcaccgct tctgggttat ggttttatct
     1441 catccatact tatctctttg ccactggcta tattatcagg atggttatac ggtattatgc
     1501 tgaataaaat taaaggttct gagatgttag tagctactta caccgggttt tctatagtat
     1561 cttttttaca gattggatgg gtcaccttgc ccttcactca tccggaaatg agatggccta
     1621 taggtaaagg attaaga
//
LOCUS       DAOQWT010000405         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_92058_length_1633_cov_3.352978, whole genome shotgun sequence.
ACCESSION   DAOQWT010000405 DAOQWT010000000
VERSION     DAOQWT010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92058_length_1633_cov_3.352978"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="PKJ95_07320"
     CDS             <1..1222
                     /locus_tag="PKJ95_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HNR66082.1"
                     /translation="SNLSKIEPGNSIVKNNRFIYYLQKKNWQKISPLVALIHPEQLLN
                     KKQEINILRRPEHIEQEFSEFPGWIVDKIKNREVGLCNYSYPRWKDRLDWKLPEQWNI
                     NIVGLGNVGGTLLVALRSMSGEKVNKIGIFDTNEKSMSRWEQETNQIIDGSGQRKFPP
                     VKIVKSEEIFQADLVIFCISMGIPSPEQGFQDITKVQLEANSKIIDYYAQRARKNNFN
                     GIFAVVSEPVDLLCQRALLASNLSEEGKFDGKGLAADQIRGLGLGVMNGRAVYYAEKE
                     KDIRHYVSKGRVFGPHGIGLVVADDVENYDRGKSSYLREKVLLATSRLIKIGFKLYIA
                     PAVASGAFSILNLMEGKWHYSAAFLGEIFWGSRNRQTPVGLEWEQYNLSPELLEDLDD
                     CFQNLKKQAKNLSK"
     gene            1362..>1633
                     /locus_tag="PKJ95_07325"
     CDS             1362..>1633
                     /locus_tag="PKJ95_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNR66083.1"
                     /translation="MKALLIIDVQNDFIKGGTLAVPDAELVIPVINDLMQKFKLVLAT
                     QDWHPKETAHFEEWPVHCVAGSKGADFPSDFHKEKIETKKNYENHK"
BASE COUNT          555 a          248 c          360 g          470 t
ORIGIN      
        1 aagtaactta tcaaaaatag aaccaggtaa ctcaattgtt aaaaataata gatttattta
       61 ttatctccag aaaaaaaact ggcagaaaat cagcccttta gtagctctta ttcatcctga
      121 acagttatta aataagaaac aagaaataaa catcttacga agacctgagc atattgaaca
      181 agaattttct gaatttcccg ggtggattgt tgataagata aaaaacagag aggtcggtct
      241 ttgtaattat agctatccac gctggaaaga tagattagac tggaaattac cggaacaatg
      301 gaatattaat attgtaggac tgggaaacgt gggaggaaca ttgcttgttg ccctgcgttc
      361 tatgagtgga gaaaaagtga ataaaattgg gatatttgac accaatgaga aatcaatgtc
      421 ccgctgggag caggaaacaa accagattat agatggttct ggtcagagaa aattccctcc
      481 cgtgaaaatt gtaaaatctg aagaaatatt tcaggcagat ctggtaattt tttgtatctc
      541 tatgggcatt ccttcccctg agcaggggtt tcaggatatt accaaagtac agctggaagc
      601 caattcaaaa ataattgatt attatgccca gagggctaga aaaaacaact ttaatggcat
      661 ctttgctgtt gtatctgaac cggttgattt gctttgccag agagctttgt tagccagcaa
      721 tctcagcgag gaagggaaat ttgatggaaa gggacttgct gcagaccaga ttagagggct
      781 gggattgggg gttatgaatg gacgggcagt ttattatgct gagaaagaaa aagatatcag
      841 gcattatgta agtaagggac gggtatttgg acctcacggt atcggtttag tagtggctga
      901 tgatgtggaa aactatgacc ggggaaagtc atcctatctg agggaaaaag tactgcttgc
      961 tacttcgcgg ttaataaaaa ttggctttaa actttatatt gcgccagcag tagcctctgg
     1021 tgctttctcc attttaaatc taatggaagg gaaatggcat tatagtgcag catttttagg
     1081 tgaaatattc tggggttcac gaaatcgtca aacaccagtt ggtttggaat gggagcaata
     1141 taacctctct cccgaactgc ttgaggattt agatgattgt tttcaaaatt taaaaaagca
     1201 ggcaaaaaat ttatcaaaat agtccgatta tttgtgcacc tcattccagg aaagatgtct
     1261 tttgctcgta gcatttgcct gcagattact ttacaagcat aatgaaggaa attgatggag
     1321 taataatttt tatatctttt atataaagaa aggataacta tatgaaagca ttattaatta
     1381 tagatgtaca gaatgatttt ataaaaggtg gaacattagc ggtaccggat gccgaactgg
     1441 ttattcctgt tattaatgac ttaatgcaaa agtttaaact ggtactggca acacaggact
     1501 ggcatccaaa agagactgct catttcgaag aatggccagt acattgtgtt gctggcagta
     1561 agggggctga ttttccctct gattttcata aagaaaaaat agaaaccaaa aaaaattatg
     1621 aaaatcataa aaa
//
LOCUS       DAOQWT010000406         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_92711_length_1626_cov_3.220242, whole genome shotgun sequence.
ACCESSION   DAOQWT010000406 DAOQWT010000000
VERSION     DAOQWT010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92711_length_1626_cov_3.220242"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /gene="murJ"
                     /locus_tag="PKJ95_07330"
     CDS             <1..1020
                     /gene="murJ"
                     /locus_tag="PKJ95_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNR66084.1"
                     /translation="MVGGLAQVLIHIPSLIKKQFHYQLTLNIHDDEVRVLFQILIPAI
                     FGLAIDKINFAVDRIIASYLAHGSISALYYANRLMQFPLGVFAIALSIAILPTLSRHI
                     AKGEIDKMKDSFSFGYKLLSFFTFPSTVGLIVLSYPIVRLFYQHGLFSSQDTSLTEVA
                     LVCYSIGLFATAILRLVVSSFYALKDTRTPLRIGIFVVVFNIILDLILVRYLAHAGLA
                     LATSVSAILHLVILSISLDKKVQGIYNRSLLFFTLKTLLACILMAFTCWEIARYFDYR
                     FDMNLKIFQLSQVFISGLIGIIVYYLSGIIMGIPEFRNGRKMLKAIMSRKMKEINEND
                     PINYN"
     gene            998..>1626
                     /locus_tag="PKJ95_07335"
     CDS             998..>1626
                     /locus_tag="PKJ95_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNR66085.1"
                     /translation="MTQLITTENIRNFSIIAHIDHGKSTIADRILELTEAISQREKRE
                     QILDNMDLEREKGITIKSKAVRLLYKAMDGKQYIFNLIDTPGHVDFSYEVSRNLAACE
                     GALLVVDAAQGVQAQTLSNVQMALDNNLSIIPVINKIDLPTADPEKVSKEIKKIEGLE
                     NNPIFLVSAKDGTGIREMLEAVIKYIPHPKGSEDKPLQALIFDSVYNSY"
BASE COUNT          469 a          295 c          319 g          543 t
ORIGIN      
        1 atggttggcg gattagcgca ggtattgata catattccct ctttaattaa aaagcagttc
       61 cactatcaat tgactttaaa tattcacgat gatgaagtca gggtactctt tcagatatta
      121 atccctgcta tttttgggtt agccattgat aagattaatt ttgctgttga ccggattatt
      181 gcctcttatc tggcacatgg gagtatatca gcattgtatt atgctaaccg gcttatgcag
      241 tttccccttg gcgtttttgc catagccctt tctattgcca tacttcctac cctctcccga
      301 catattgcca aaggcgaaat agataagatg aaggatagtt tttcttttgg ttataaactt
      361 ctttcctttt ttacctttcc ctccacggta ggattaattg tgcttagcta ccccattgtc
      421 agactatttt accagcatgg tttgttcagt tcccaggata cttctcttac tgaagtagca
      481 ctggtctgtt attctatcgg tctttttgct actgccattt tgagattggt ggtaagctcc
      541 ttttacgcct tgaaggatac cagaacgcct ctaagaatag gtatctttgt agtggtgttt
      601 aatattatct tagatttaat cttagttcgt tatttggctc acgctggatt ggcattagca
      661 acctcagtct cagctatttt gcatcttgtt attttaagta tttctttaga caagaaagtg
      721 caaggtatat ataatagaag tctcctcttt ttcaccttaa aaacactgct ggcttgtatt
      781 ttaatggctt tcacctgctg ggaaattgcc cgttattttg attatagatt tgatatgaat
      841 ctaaaaatat ttcagttgag ccaggttttt atttccggtc tgataggtat aatagtatat
      901 tatctaagtg gtataataat gggtatcccg gaatttagaa atggcaggaa aatgcttaag
      961 gcaattatga gtagaaagat gaaggaaatt aatgaaaatg acccaattaa ttacaactga
     1021 aaatattcgt aatttttcaa ttattgccca tattgatcac ggaaaatcca ccattgcgga
     1081 ccgcattctg gagcttaccg aggcgatcag tcaaagagaa aagagagaac agattcttga
     1141 taatatggat ttagaaagag aaaagggtat cactattaaa tcaaaagctg ttcggctatt
     1201 gtacaaagct atggatggga aacaatatat cttcaattta attgatactc ccggccatgt
     1261 tgatttctcc tatgaagtat cccgtaatct ggcagcctgt gaaggcgctc tactggtagt
     1321 agatgccgcg cagggtgttc aagctcaaac gctgtccaat gttcagatgg ctctggataa
     1381 caatctttct attatacctg taatcaataa gattgattta cccaccgctg atccggagaa
     1441 agtcagtaaa gagattaaaa aaattgaagg attggaaaac aatcctatat tcctggttag
     1501 cgccaaagat ggcaccggaa taagggagat gctggaagct gttattaaat atattcccca
     1561 tccgaagggt agcgaggata aaccactgca ggcgttaatc tttgattcag tttataattc
     1621 atatca
//
LOCUS       DAOQWT010000407         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_92899_length_1624_cov_3.372849, whole genome shotgun sequence.
ACCESSION   DAOQWT010000407 DAOQWT010000000
VERSION     DAOQWT010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92899_length_1624_cov_3.372849"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1320)
                     /gene="serS"
                     /locus_tag="PKJ95_07340"
     CDS             complement(46..1320)
                     /gene="serS"
                     /locus_tag="PKJ95_07340"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNR66086.1"
                     /translation="MLDLKLIRSEPDQVKAALKKRGQDTGSIDYILEIDGKRRKIIFD
                     STALKHERNEVSEEIARMKKEKKDVQAKIAAMREVSARIKEMDNEIRSIEQEIQNILL
                     EIPNIIDDSVPVGANEESNQEVRRWGKPREFDFEPKAHWELGEKLDILDFERGAKVSA
                     ARFTVLKNEGARLERALINFMLDIHTREHNYQEILPPFLVNAQTMMGTGQLPKFENEL
                     FKTNDELYLVPTAEVPLTNLHQGEILPESVLPIYYTAYTACFRREAGSYGKDIRGLIR
                     QHQFDKVELVKISKPADSFLELEKLTQNAEVILQRLELPYRMVVLSTGDTGFSAAKTY
                     DLEVWLPYQGKYREISSCSNCTDFQARRANIRFREEESGKVNFVHTLNGSGIAVGRTM
                     VAIIENFQEQDGSIVIPDALRPYMNGLQKISS"
     gene            complement(1376..>1624)
                     /locus_tag="PKJ95_07345"
     CDS             complement(1376..>1624)
                     /locus_tag="PKJ95_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNR66087.1"
                     /translation="GSTCHGAGRVLSRGAAIKAVRNRNIAQELRERNIIVKARSRDTL
                     AEEMPEAYKDVSEVVNVMDQSGISSKVARMRPLGVIKG"
BASE COUNT          435 a          385 c          247 g          557 t
ORIGIN      
        1 cctccgcaaa tgacctcaag aaatagacag cattaaaatt ttaccctaac tggaaatttt
       61 ttgcaatcca ttcatatagg gacgcagggc atctggtata acgattgaac catcctgttc
      121 ctgaaaattt tctataatag caaccatcgt tctgcccacc gctattccgg aaccatttaa
      181 ggtatgcaca aaattaacct tgccggattc ttcttcccga aaacgaatat tggctctgcg
      241 cgcctgaaaa tcagtacagt tactacagga ggatatctct ctgtattttc cttgatatgg
      301 caaccatact tctaaatcat aagtcttggc agccgaaaag cccgtatctc ctgtagatag
      361 aactaccata cgatagggta attctaatct ttgcaaaata acttccgcat tttgagttag
      421 cttttccagc tcaagaaaag aatctgctgg ttttgaaatt tttaccaatt ccaccttatc
      481 gaactgatgc tgtcttatta atcctcttat atctttacca taagagcctg cctctcttct
      541 gaaacaggca gtataagctg tataataaat tggcaaaact gattctggca atatttcgcc
      601 ctgatgcagg ttggttaagg gaacttccgc tgtaggaacc aaatataatt catcatttgt
      661 tttgaatagt tcattttcaa attttggtaa ctgaccggta cccatcatag tctgagcatt
      721 taccaagaaa ggcgggagta tttcttgata gttatgttct ctggtatgaa tatctagcat
      781 aaaattaatt aaggctcgct ccagtcttgc tccctcattt tttaaaacgg taaagcgtgc
      841 tgcagaaact tttgctcctc tttcaaaatc aagaatatcc agtttttcac ccaattccca
      901 atgtgctttt ggctcaaaat caaattctct gggtttaccc catcttctta cttcctggtt
      961 actctcctca ttggctccaa ccggtacact atcatcaata atattgggaa tttctaatag
     1021 tatattttga atctcttgct ctatagacct tatttcgtta tccatctctt ttattctggc
     1081 agatacctct cgcatggctg caatttttgc ctggacatct tttttttctt ttttcatgcg
     1141 ggcaatctcc tcagatacct cattcctctc atgttttaag gctgttgagt caaagataat
     1201 cttccggcgt ttaccatcaa tttctaatat ataatcgata ctaccagtat cctgccccct
     1261 cttcttcagg gctgctttta cctgatccgg ttctgaacgg attaatttta aatccaacat
     1321 cttcttaaaa accctcctga aactatgtta ttgctatttc ttaaacaatt ttttattatc
     1381 cttttataac tcccaatgga cgcattctgg caactttgct ggaaatgcca ctctggtcca
     1441 tcacatttac cacttctgaa acatctttat aggcttcagg catttcttca gccagagtat
     1501 ccctacttct ggctttgact ataatatttc tttcccttaa ttcctgagca atattcctat
     1561 ttctaactgc ctttatagca gcccctcgac ttagcactct accggcaccg tgacaggtag
     1621 aacc
//
LOCUS       DAOQWT010000408         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_92906_length_1624_cov_3.079669, whole genome shotgun sequence.
ACCESSION   DAOQWT010000408 DAOQWT010000000
VERSION     DAOQWT010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92906_length_1624_cov_3.079669"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..>1624
                     /locus_tag="PKJ95_07350"
     CDS             65..>1624
                     /locus_tag="PKJ95_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR66088.1"
                     /translation="MDSNNIKDRLAIIAMPGHVLFKGIKSKIRIDRAMAESIYPRIKQ
                     DNDYAIVLLLKEGCNLDDYQEKDFYTVGSLIKIGKIANSKRTSDLNVEVLDRVEVKNI
                     SREQDYYIAEYDISPDIDDLDKNSQEQMLEYIKKISTEISKGFIGAESYVEYINNIDD
                     LEKIISFLMQFISISAPEKQELFEIRSRRKKCLMFLDILIKQKENIQFQMEINAKLSD
                     EVNKQYREKMLREQLKAIQDELSEGQQSGNKKKDYLTLINESKMPEDVKEIAREENEK
                     LENQGPNSAETNVIRNYLDLLTSLPWGKSSAKEVDIKEAKKILEQDHYGLEKVKERII
                     QHLTVMKLKNNQQGSILLLVGPPGTGKTSLGKSIAKALQREYVRISLGGVRDEAEIRG
                     HRRTYVGALPGRIIQGIKKAGTNNPVFILDEVDKLLASNMGDPTSALLEVLDPEQNNT
                     FSDHYLEVPYDLSEVFFIGTVNSLRDIPEPLKDRMEIIQINSYTTAEKFHIAKEHLIG
                     EVLESHGLKDEQ"
BASE COUNT          610 a          218 c          384 g          412 t
ORIGIN      
        1 aagattatta atatttgatg ataggtgtaa agagtaattc agttatagaa gtgaggtaag
       61 attaatggat agcaataata taaaagatag attagctatt atagccatgc ccggacatgt
      121 gttgttcaaa ggaataaaga gcaagataag aattgatagg gcaatggctg aatcaatcta
      181 tcccaggatt aagcaggata atgattatgc catagtactg cttctaaaag agggatgtaa
      241 tctggacgat tatcaggaaa aagattttta tacagttgga agcctgataa agattgggaa
      301 gatagctaat tcaaaaagaa ccagtgattt gaatgtagag gtattggata gagtagaggt
      361 aaaaaatata agcagggaac aggattatta tattgctgaa tatgatatca gccctgatat
      421 tgatgatttg gataagaatt ctcaagagca gatgctggaa tatattaaga agatttctac
      481 tgagattagc aaaggattta tcggtgctga aagctatgtc gaatatataa ataacataga
      541 tgatttagaa aaaatcatca gttttttaat gcagtttatc agcatctctg cacccgaaaa
      601 gcaagagcta tttgagataa ggtcaagaag aaaaaaatgt ctgatgttct tagatattct
      661 cattaagcag aaagaaaata ttcaattcca gatggagata aatgcaaaat taagcgatga
      721 agtgaacaaa caatatagag aaaaaatgct gagggaacag ctaaaggcga tacaggacga
      781 gttgagcgag ggacagcagt ctggaaataa aaagaaagac tacttaacct taattaatga
      841 gtctaaaatg cctgaagatg tgaaagagat tgcccgggaa gaaaatgaaa aattggaaaa
      901 tcaaggtccg aatagtgcgg agacgaatgt aataagaaat tatttggatt tgttgacttc
      961 actcccctgg ggcaaaagca gtgcaaaaga agtagatatt aaagaggcga aaaagatttt
     1021 agaacaggat cactatggcc tggaaaaggt gaaggagaga attattcagc acttaactgt
     1081 aatgaaactc aagaataatc aacagggttc aattctactt ctggtgggac ctcctggaac
     1141 cggtaagacc agcctgggta agagtattgc caaggcactt caaagagaat atgtgagaat
     1201 aagtcttggt ggagtgaggg atgaagcgga gataagaggg cacaggagaa catatgttgg
     1261 cgcactccct gggagaatta ttcagggaat taaaaaggca ggaaccaata atcctgtctt
     1321 tatactggat gaggtggata agttactggc ttctaatatg ggagacccaa ccagtgcatt
     1381 gttagaagta ttggaccccg agcagaataa tactttttca gaccattatc tggaagttcc
     1441 ctatgacctg tctgaggtat ttttcatagg gacagtaaat tctctgaggg acatcccgga
     1501 accattgaag gatagaatgg aaattattca gataaatagc tataccacgg cagagaaatt
     1561 ccatatagct aaggaacacc ttatcggtga ggttttagaa agccatggac ttaaagatga
     1621 acaa
//
LOCUS       DAOQWT010000409         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_93788_length_1615_cov_3.751923, whole genome shotgun sequence.
ACCESSION   DAOQWT010000409 DAOQWT010000000
VERSION     DAOQWT010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93788_length_1615_cov_3.751923"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..586
                     /locus_tag="PKJ95_07355"
     CDS             191..586
                     /locus_tag="PKJ95_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66089.1"
                     /translation="MKEEIKQVLDMLREEKISNEDAVNLIDALKSTDSEENEQELFGA
                     TKQKKYIRINITKDDKPAVNIKVPFGLIRWGINIANKLGKEAGEIGGKEIPIDLDELS
                     HALVDPDFYGKIIDVYDEEKNEKVIIEIV"
     gene            complement(601..>1615)
                     /locus_tag="PKJ95_07360"
     CDS             complement(601..>1615)
                     /locus_tag="PKJ95_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNR66090.1"
                     /translation="RIFLNGVDTGKVTPALIDNIYRGSHTIYLTLDNPAMSKTELVVV
                     ERGQTTSVNIELLAETRYRALLVGIDKYKEPGVMNLIAPPYDVDRIEQVFERAHFGDR
                     KVSFSVLNTLVGAQATRSNIFEGITSTFSQAGNDDVSYFYYSGHGWVKDGKSTILPHD
                     AKNFDDSKDITVEELAEALGSIPGTKIVIMDACYSGGFIGKELYSWGIAGSEGLQEFN
                     ANVIDTFASYDLFGLESVKGNLATQGFQVIVSATGNQQCFETINPHPLDGNPYGYFSR
                     FLCEGCGYNSFNFPYPADQNRNRKVTINEIYQYICTEISHTHVKQDAQVYPLNSSFTF
                     IEY"
BASE COUNT          551 a          294 c          312 g          458 t
ORIGIN      
        1 ctcttaccct acggtaaaga atagactgga taagctatta gcagcattgg gtctactgga
       61 agaagttaga accattagtc aaagtgaaat tattgccgct ctgagagatg gagatatttc
      121 agtcgaagaa gcaattaatt tattaaagga aaataatttt aaaacataaa ttaaattcaa
      181 ggagaagaag atgaaagagg aaatcaaaca ggttctggat atgttaaggg aagaaaaaat
      241 tagcaatgaa gatgctgtta accttattga tgccctaaaa tcaacagact cggaagagaa
      301 tgaacaggag ctttttggtg ccacaaaaca aaaaaagtat attagaatta acattaccaa
      361 agatgataag cctgctgtta acatcaaggt cccttttggt ctgataagat ggggaattaa
      421 tattgccaat aagttaggta aggaagctgg ggaaatcggt gggaaggaaa ttccaattga
      481 cctggacgaa cttagccatg ctttagttga ccctgatttt tacggtaaga ttattgatgt
      541 ttatgatgaa gaaaaaaacg aaaaggtaat catcgaaata gtgtaatgct tgtccttgat
      601 ttaatattca atgaaggtaa aagaggagtt tagaggatat acctgggcgt cctgttttac
      661 atgagtatga gatatttcag tacaaatgta ctggtaaatt tcatttatgg taacttttct
      721 atttctgttc tggtcagctg ggtagggaaa attaaaacta ttgtaaccac aaccctcgca
      781 gagaaatcta gaaaaatagc cataaggatt gccatcgaga ggatgtggat tgattgtctc
      841 aaaacactgc tggttgcctg tagcagaaac gataacttga aatccttgtg tggctaaatt
      901 accctttaca gattctaatc caaataaatc atatgaggca aaggtatcga tgacattagc
      961 gttaaattcc tgcaaacctt cactgccagc aattccccag gaataaagtt cttttccaat
     1021 aaaaccaccg gaataacagg catccattat aacaattttt gtccccggaa tacttcccaa
     1081 tgcttctgct aattcctcaa cagttatatc tttggaatca tcgaaattct tcgcatcgtg
     1141 aggtaagatg gtagatttac catctttaac ccagccatgt cccgaataat aaaagtaaga
     1201 gacatcgtca ttaccagctt gagaaaaagt ggaggtgatt ccctcaaaga tattagaccg
     1261 agtagcctgt gcgcccacca atgtatttaa tacagaaaaa gataccttcc tatcaccaaa
     1321 gtgagccctc tcaaaaacct gctctattct atccacatca taaggtggtg ctattaaatt
     1381 cataacaccc ggttctttat atttgtcgat tcctaccaac agcgcccggt atcttgtttc
     1441 agctaatagt tctatattca ctgaggttgt ttgaccccgt tccacaacta ccaattcggt
     1501 tttactcata gcaggattat ccagagtcaa ataaatcgtg tgagaacccc ggtaaatatt
     1561 atcaataaga gcgggggtaa ctttacccgt atcaacaccg ttaagaaaaa ttctc
//
LOCUS       DAOQWT010000410         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_93913_length_1614_cov_2.828095, whole genome shotgun sequence.
ACCESSION   DAOQWT010000410 DAOQWT010000000
VERSION     DAOQWT010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93913_length_1614_cov_2.828095"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PKJ95_07365"
     CDS             complement(<1..619)
                     /locus_tag="PKJ95_07365"
                     /inference="COORDINATES: protein motif:HMM:NF040244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HNR66091.1"
                     /translation="MQDRLKSVLSKQRADYVEVRLEEYESTNIRFQGKDIDKISSSQE
                     KGGNARALVKGRWGFVTFNRWEDLEDKVKQAVEIATLIGDEQSYLASMEPVVEDLMAE
                     YPNYFREVNLKEKVALMKNYSNIITNFSSKIQSSIADYTDRYNVVHFANSEGTYIRQV
                     HPYLSVHFTAIARDGDDVQMAKESIGTVKGYEQALQISSLAQKAAQ"
     gene            complement(716..1138)
                     /locus_tag="PKJ95_07370"
     CDS             complement(716..1138)
                     /locus_tag="PKJ95_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNR66092.1"
                     /translation="MSAALLHDIGKVEEYQLRGIIDHTDKGKLIGHIIMGAEMIEEKI
                     KLIKDFPEDLAMMFKHTILSHHGKLEFGSPKLPSILPALALFYADDTDAKLNGLVCLK
                     EENKYLDKKWSDWIWWLERPIFLAEDNIIKESGNRSEE"
     gene            complement(1148..>1614)
                     /locus_tag="PKJ95_07375"
     CDS             complement(1148..>1614)
                     /locus_tag="PKJ95_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66093.1"
                     /translation="GKTVDGFFKLTDSSGEIEAVYWDLSEQKIAQIEKMELAQIEGQV
                     SKKRSGGQMQISIKTIEHAPESTWEDFIPSTSFDIEQIMQGIDDKIASITHPHLKALL
                     DSFFKDAVLLEKFKKSPAAKKLHQAYRGGLAEHTLNVCQICETICQIYPQIS"
BASE COUNT          428 a          354 c          261 g          571 t
ORIGIN      
        1 gttgtgctgc tttttgtgct aaagaagata tttgcagtgc ctgttcatat cccttaacag
       61 tacctatgct ctctttggcc atctgaacat catcaccatc acgggcaatg gctgtgaagt
      121 gtacactaag ataagggtgt acttgtctga tataggtgcc ttcagaatta gcaaaatgca
      181 ccacattata gcggtcggta taatcggcta ttgatgattg tattttggag ctgaaattgg
      241 ttattatatt gctataattt ttcatcaaag ctactttttc ttttagattc acttctctaa
      301 aatagttggg atattcagcc attaaatctt ccaccaccgg ttccattgaa gccaaatagc
      361 tctgctcatc tccaattaag gtggcaattt caacggcttg ttttacctta tcttctaaat
      421 cttcccagcg attaaaggta acaaaacccc atcgaccctt taccaatgcc ctcgcatttc
      481 ctcccttctc ctgggagctg ctgattttgt ctatatcctt tccttgaaaa cgaatattgg
      541 ttgactcata ttcttccagt cttacctcca cataatctgc ccgttgtttc gatagaacac
      601 tttttagcct atcctgcata gttttcctcc tgctctaata ttgtttgttt gttgtgttta
      661 caatttttta tatatgtttg cgtattacta ttaaaattaa aaagagctgc ttttactatt
      721 cttctgatct gttaccactc tcttttataa tattatcttc tgccaggaaa ataggtcgct
      781 ccagccacca gatccagtca ctccattttt tatccagata tttattttct tcttttaaac
      841 agactaatcc attgagcttg gcatcagtat catcagcata aaaaagagcc agagctggca
      901 atatgctggg cagtttgggt gagccaaatt ccaatttacc atggtggctt aaaatggtgt
      961 gtttgaacat cattgccagg tcttccggaa aatcctttat caatttaatc ttttcctcta
     1021 tcatctcggc tcccataata atatgtccga ttaacttacc tttatcagta tggtcaataa
     1081 tccccctcaa ctgatattct tccacttttc caatatcatg gagaagcgcg gcactgataa
     1141 ggaaatctca actaatctga gggtaaattt gacaaattgt ttcgcatatc tggcatacat
     1201 taagggtatg ttctgctaaa ccaccccggt aagcctggtg taatttttta gcggcaggcg
     1261 attttttaaa tttttctaat aaaactgcat ctttaaaaaa agaatccagt agtgctttta
     1321 aatgtgggtg tgtaatgctg gcaattttgt catcaattcc ctgcattatt tgctcaatat
     1381 caaaagaggt tgaaggaata aaatcctccc aggtggattc cggggcatgt tcaatggttt
     1441 ttatacttat ctgcatctgc ccaccactcc tctttttgct aacctgacct tcaatttgag
     1501 ctaattccat cttttcgatt tgtgctattt tttgttcaga cagatcccag taaactgcct
     1561 ctatctcgcc actactatca gtaagcttaa aaaaaccatc aacagtcttt ccat
//
LOCUS       DAOQWT010000411         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_94172_length_1611_cov_3.566838, whole genome shotgun sequence.
ACCESSION   DAOQWT010000411 DAOQWT010000000
VERSION     DAOQWT010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94172_length_1611_cov_3.566838"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PKJ95_07380"
     CDS             <1..323
                     /locus_tag="PKJ95_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66094.1"
                     /translation="RLKYGAVTGSGVVGLLAGLIMPQIFSEIGGSAAVMMICASFVGM
                     SSQARLPNELYVALAAVLSTFLFIYSSPYFCGTGGKLGTIAFISCIIIKGFLDILEKY
                     KNNQ"
     gene            329..817
                     /locus_tag="PKJ95_07385"
     CDS             329..817
                     /locus_tag="PKJ95_07385"
                     /inference="COORDINATES: protein motif:HMM:NF028231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-16S rRNA-processing nuclease YqgF"
                     /protein_id="HNR66095.1"
                     /translation="MKEKVILSLLESGRMNLKKSDKDTPDNVLAIDPGRKKCGIAVIN
                     NEMKLVAGKVIDKEKLIGELKFFLEKYTIKDIAIGGGTNSQEIFKIVKNHFSDIRLTT
                     VIEKDSTMLARKEYFNYNPPRGWLKLLPASLRIPPCPYDDFAAYIIARRFLLDYRKDT
                     GL"
     gene            869..>1611
                     /locus_tag="PKJ95_07390"
     CDS             869..>1611
                     /locus_tag="PKJ95_07390"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNR66096.1"
                     /translation="MKTRFKVTIYGIVQGVGFRPFIHKLVKNYHIYGWIRNSNRGVEM
                     EIEGNKFDCLKLFAELKKNTPPLTLIEKIKIKELPPLGFSEFIIKESNSDHKHPLILM
                     PPDISICQDCLRELKDPSNRRYRYPFINCTNCGPRFTIIKDMPYDRDNTTMQDFSMCR
                     DCLEEYHNLVDRRYHAEANACPVCGPQIYLYKGEHKINVTNPILEAQNRLKKGEIGVI
                     KGLGGFHLACDAQNREAVSRIKNIKKRDQ"
BASE COUNT          543 a          243 c          336 g          489 t
ORIGIN      
        1 taaggcttaa atacggggca gttacaggtt caggagttgt aggcttgtta gcaggactga
       61 ttatgcctca gattttttct gaaatagggg gaagtgcggc agtaatgatg atatgtgcat
      121 catttgtggg gatgtcttca caggccaggc tcccaaatga attatatgtt gcccttgctg
      181 ctgtattgag tacctttctt tttatctaca gctctcctta tttttgtgga acgggtggaa
      241 aattaggtac aattgccttc atatcgtgta ttataattaa aggatttctt gatatactgg
      301 aaaaatataa aaataaccaa taaggggatt gaaagaaaaa gtaatactat ctttgttgga
      361 aagtggtaga atgaatttaa aaaaatctga taaagatact ccagataatg ttctggcaat
      421 agatccgggt agaaaaaaat gtggtatagc agttataaat aatgagatga aattagtagc
      481 agggaaagta attgataaag agaagttgat aggggaactg aagttcttcc ttgaaaagta
      541 tacgattaaa gatatagcca taggcggagg aactaattct caggagattt ttaaaattgt
      601 aaaaaatcat ttttcagata taagattgac cactgtaatt gaaaaagata gtaccatgtt
      661 ggccagaaaa gagtatttta attataaccc accaagagga tggctgaaac ttttacccgc
      721 ttctcttcgt atacctccct gtccatacga tgattttgct gcttatataa ttgccagaag
      781 gtttttattg gattaccgga aagataccgg actgtagagc aatatccagt caagtttacc
      841 ctatatttat ataagtttta aggaaaagat gaagacaaga ttcaaggtta ccatatatgg
      901 aatagtgcag ggggtgggat ttcgaccttt tattcataaa ttagtaaaga attaccatat
      961 ttatggctgg attcgaaatt ccaatagagg agttgaaatg gaaattgagg ggaataaatt
     1021 cgactgctta aaattatttg ctgagctgaa aaagaatacc ccacccttaa ctcttattga
     1081 aaaaataaaa attaaagagt tgcccccttt aggtttttcc gaatttatca taaaagagag
     1141 taactctgac cacaaacatc cccttattct tatgccccct gatatctcca tctgtcagga
     1201 ttgtttgaga gagttaaaag atccatctaa ccgtcgctac cgttatcctt ttatcaattg
     1261 taccaattgc ggaccgcgtt ttaccattat taaagatatg ccttatgata gagataacac
     1321 tactatgcag gatttttcta tgtgtagaga ttgtcttgag gagtatcata atttagttga
     1381 tcgtcgctac catgcagaag ccaatgcttg cccggtttgt ggtccacaaa tttatctata
     1441 taaaggggag cataaaatca atgttaccaa tcctattctg gaagcgcaaa acaggttaaa
     1501 aaaaggagag ataggggtaa taaaagggtt agggggtttc catcttgcct gtgatgctca
     1561 gaatagagag gcggtttcca gaataaaaaa tattaagaaa agagatcaga a
//
LOCUS       DAOQWT010000412         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_94190_length_1611_cov_2.901671, whole genome shotgun sequence.
ACCESSION   DAOQWT010000412 DAOQWT010000000
VERSION     DAOQWT010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94190_length_1611_cov_2.901671"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PKJ95_07395"
     CDS             complement(<1..771)
                     /locus_tag="PKJ95_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNR66097.1"
                     /translation="MLYIGTSGFSYQDWIGPFYPENIAKGDMLTLYAREFNTVEVNSS
                     YYRIMPATVFYHLQNKVPADFKFTVKANQVITHTRDKNEAVFQEFKASLQPILKNNKL
                     GCILAQFPYSFHYNHDNLDYLLYVKEKMDYAPLVVEFRNNYWLKDSIFDFLRKNEIGF
                     CMVDQPALKGLIPPLAVVTSQLAYLRFHGRNKEKWWQHEYAYQRYDYLYSEQELEEWV
                     PKIKEIIGQTSDQYIFMNNHYKGKAVKNALMLMGLLRQE"
     gene            complement(782..1390)
                     /gene="lexA"
                     /locus_tag="PKJ95_07400"
     CDS             complement(782..1390)
                     /gene="lexA"
                     /locus_tag="PKJ95_07400"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNR66098.1"
                     /translation="MTLDKEVLTKKQKVALEVIRIKTQQTGYPPTVRELCKALGVNSS
                     ATAFKYLKILEKKGYIKREKEKTRAIKLIPQTRNLPLIGKVAAGNPLLALQEYTEVLP
                     VPEELAGKEGSFLVRVEGDSMLGDHILDGDYVIVHPQNNADNGDIVIALLNQEEVTVK
                     RFYQNKDKITLKPSNPAYQPIITREVAILGKVTGIIRRFNGR"
BASE COUNT          467 a          331 c          310 g          503 t
ORIGIN      
        1 ctcctgccgc agcagaccca tcagcatcaa agcgtttttg accgctttcc ccttataatg
       61 attattcata aagatgtact ggtcactggt ctggccgata atctctttaa ttttaggtac
      121 ccattcctct aattcctgtt cactgtagag ataatcataa cgctgatagg catactcatg
      181 ctgccaccat ttttctttgt tacgtccatg aaaacggaga taagccagct gtgaggttac
      241 tacagctaaa ggaggaatta atcccttgag agccggctgg tctaccatac aaaatcctat
      301 ttcatttttt ctgagaaaat caaatatcga atcttttagc cagtagttat tgcgaaattc
      361 cactaccaga ggggcataat ccatcttttc ctttacatag agtagatagt cgaggttatc
      421 gtgattataa tgaaaagaat aggggaattg tgccaagatg cagcccaatt tattattttt
      481 cagaattggc tgcaaagagg ctttaaattc ctgaaataca gcttcattct tatcccgggt
      541 atgggtgata acctgatttg cctttactgt aaatttgaaa tcagccggga ccttattctg
      601 aagatgataa aaaacggttg caggcataat tcggtaatag gaagaattga cttccactgt
      661 attgaactct cgggcataga gagtcaacat atctcctttt gcgatatttt cgggataaaa
      721 tgggccaatc cagtcctgat aactgaaacc ggaagttcct atatagagca taattgttta
      781 attacctccc attaaatcta cgaatgatgc ccgttacctt gcccaagatt gctacttctc
      841 tggtaatgat aggctgatag gcaggattgg aaggtttcaa ggttatttta tccttatttt
      901 gataaaacct ctttacagtt acctcttcct ggttgagcag ggcaataaca atatccccat
      961 tgtcagcatt attctgggga tgaactataa cataatcccc atccaggata tggtcaccga
     1021 gcatactgtc tccttccacc cgaaccagaa aactcccttc tttgccggct aattcttccg
     1081 gcaccggaag cacttctgta tattcctgta aagcgagcag gggattgcca gcggcaactt
     1141 tgccaatcag ggggagattg cgggtctggg gtattaattt gatagccctt gttttttcct
     1201 tttccctctt gatatagcct ttcttttcca aaattttcag gtatttaaag gcagtagcag
     1261 aggaattgac acccagggct ttacataatt cccgaactgt tggaggataa ccggtctgct
     1321 gagttttaat acgaataacc tctaaagcca ccttctgttt tttggtaagc acttctttat
     1381 ccagagtcat gcctttttcc tcacaaattt tttatacaat atctggtaat cacattgtaa
     1441 ggcaaacata tgtttgtgtc aagtaaaaga tattataaat tataaaatct gaaatatacc
     1501 ggtgggacaa aatgagtata attctgcagc cggagaaata ggtcttgacg caatcagagt
     1561 ataaagaaag agtcggttac ctctatagga ttgataaaat tatcatacat a
//
LOCUS       DAOQWT010000413         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_94506_length_1608_cov_3.049581, whole genome shotgun sequence.
ACCESSION   DAOQWT010000413 DAOQWT010000000
VERSION     DAOQWT010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94506_length_1608_cov_3.049581"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="PKJ95_07405"
     CDS             complement(<1..1286)
                     /locus_tag="PKJ95_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNR66099.1"
                     /translation="MDIKQKYDKYVNLYFINKMEDVVIDHAEGSTVYATDGKEYIDCF
                     AGIAVNNAGHRNPKVIQAAKDQMDKLVHCCAYVYYSEPPAILAEKLAQITPGKLDKTF
                     FSNSGAEAIEGALRVAKQFTGNYEIIALQCSFHGRTNATLSVTGNRKRKFKGGPFLPG
                     IAFAPAPYCYRCPFELKRESCNLKCARYLRDVIDFQTSGSVAAFIAEPVMGEGGIIVP
                     PEDYFKEIKKVLDEFNILLITDEVQSGFGRTGKLFAIEHYGVEPDIMTMAKGIADGFP
                     LGAFITRDEIASSFKPGDHLSTFGGNPVSCAAAIANIDFLVDEKIPEKALEKGNRFMQ
                     NVKDKLMKKYDLIGDVRGKGLMIGVELIKDKEKTPASAEAGKVRNLCREKGLLIGVGG
                     VLGNVLRIQPPLVIEERQLTKSLIYWMMPFQSLKSN"
BASE COUNT          477 a          328 c          254 g          549 t
ORIGIN      
        1 taattactct tcaagctttg aaaaggcatc atccaatata tcaatgactt tgtcaattgc
       61 cgttcttcta taaccaaagg cggttgaatt ctcagtacat ttccaagaac tcctcctaca
      121 ccaatgagta ggcctttttc tctgcataaa ttcctgactt tgcctgcttc tgcactagca
      181 ggagtttttt ctttatcttt aattaactct actccgatca tcaagccttt ccctctcaca
      241 tcaccaatca agtcatattt tttcataagc ttatctttaa cattctgcat aaaacggttc
      301 cctttttcta atgccttttc agggattttc tcatcaacca gaaaatcaat atttgctata
      361 gcagctgcac aggataccgg gtttccccca aaggttgata agtggtcacc gggtttgaaa
      421 gagctagcta tttcatctct ggtaataaaa gcacccaatg ggaatccatc agcaattcct
      481 tttgccattg tcattatatc aggttctaca ccatagtgtt caatggcaaa tagttttcca
      541 gttcttccaa aacctgattg aacctcatct gtgattaaca gaatattgaa ttcatctaag
      601 acctttttaa tttccttaaa gtaatcttca ggaggaacga ttattccacc ttcacccatg
      661 acaggttctg caataaaggc agcaactgaa ccactggttt gaaaatcaat aacatctctt
      721 aaatatctgg cacattttaa attgcagctc tctcttttca attcaaaggg gcaacggtaa
      781 caatatgggg caggagcaaa agcgattccc ggtaagaatg gtccaccttt gaattttctt
      841 tttctgtttc cagtaacact gagggtggca tttgttctac catggaaaga acattggaga
      901 gctataattt cataatttcc agtgaattgt ttggcaactc ttaaagcccc ttctatagct
      961 tcagcaccac tattagagaa gaatgtttta tcaagtttgc caggggtaat ttgagctaat
     1021 ttttctgcca gtattgcggg cggttcagaa tagtagacat atgcacagca atgaaccaac
     1081 ttatccatct gatctttagc agcctgaata acttttgggt tcctgtgccc ggcattgtta
     1141 acagcaatgc cagcaaaaca atcgatgtac tcttttccat ctgtagcgta tactgtagat
     1201 ccttcagcat ggtcaatgac tacatcttcc atcttgttga taaagtacag gttgacatac
     1261 ttgtcatact tttgcttaat gtccatacct tcatttaacc tctcttcttt tattttttta
     1321 attctattat ttaaggtaat ttaaaatacc tatgtattac aattgtaaat atttcgtaat
     1381 taaggttaaa gaaatgctat caaccaaaaa ctataatttt aaatagagac ctgttagtgt
     1441 aagcatttat agaatggtca gtatgcgtac tgttactgtt atttagtttt taagaagtaa
     1501 aaaagaattt ttatggatac gtatattata caaaaatcgt atttatacta ctatccctag
     1561 ttttgcagta agccaatgag gtcaagttct ataaatcctt gcaaactg
//
LOCUS       DAOQWT010000414         1606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_94734_length_1606_cov_2.308833, whole genome shotgun sequence.
ACCESSION   DAOQWT010000414 DAOQWT010000000
VERSION     DAOQWT010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94734_length_1606_cov_2.308833"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /gene="aspS"
                     /locus_tag="PKJ95_07410"
     CDS             <1..1433
                     /gene="aspS"
                     /locus_tag="PKJ95_07410"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNR66100.1"
                     /translation="PFNIDDHSEIGDDVRLKYRYLDLRRTEMKKNIILRHKVCLLIRN
                     FLSERGFLEIETPFLTKSTPEGARDYIVPSRVYPGKFYALPQSPQLFKQLLMISGFDK
                     YFQIVRCFRDEDLRADRAPEFTQLDMEMSFIEREDLFTLIEDLMVHLWKEVLGIEISK
                     PFPRYDYNEVVSKYGVDKPDLRYGMEIHNLNEIFRNTEFKAFSEVLSKDGKIGAIKVE
                     QPQKFSRKNIDDLEIYVKSLGLNGLISIRFKEENQIQSSLGKYITEEEVTLLKKEMQT
                     IPGDLILIVAGNSEKVNEALGQLRIKLARTLNIIPEEEYNFLWVTNFPLFEYSQTEKR
                     YSSMHHPFTSPVDEDIPMLDNNPYQARSKAYDLVLNGNEIGGGSIRIHRTEIQQKIFD
                     LLGIKHDEAVQKFGFLLEALQFGAPPHGGIAFGLDRIVMLLAGASSIREVIAFPKTQK
                     ATCLMTGAPSEVSPEQLKELHLKIDL"
BASE COUNT          502 a          262 c          356 g          486 t
ORIGIN      
        1 cccctttcaa tatagatgac catagtgaaa ttggagatga tgtgcgcttg aaataccgtt
       61 acctggattt aagaagaaca gaaatgaaaa aaaatattat attacgtcat aaagtttgcc
      121 ttttaatcag aaacttcttg agtgaaagag gctttttaga gatagagaca ccatttttaa
      181 ctaaaagcac gcctgaagga gctcgtgatt acattgtccc cagcagagta taccctggta
      241 aattctatgc tctcccccag tctccacaat tatttaagca gttacttatg attagcggtt
      301 ttgataagta tttccagata gtgaggtgtt ttcgggatga agacctgaga gcagacagag
      361 ctccggaatt tacccagtta gatatggaga tgtcctttat tgagcgtgaa gatttattca
      421 ctcttataga ggatttaatg gtccatttat ggaaagaggt cttaggtatt gagataagca
      481 aaccttttcc cagatatgat tataatgaag ttgtctcaaa atatggtgtg gataaaccgg
      541 atttgagata tggtatggaa atccataatt taaatgaaat tttcaggaat actgaattta
      601 aagccttctc agaagtgctg tcaaaagatg gaaagattgg tgccataaag gtagagcaac
      661 ctcaaaaatt ttcccgtaag aatattgacg atctggaaat ttatgtaaaa tcattggggc
      721 taaacggctt gatttctatt cgcttcaaag aagagaatca gattcagtca tctttgggaa
      781 aatatattac tgaggaagaa gtgacgttgc tgaaaaaaga aatgcagaca atacctggag
      841 accttattct tattgtggct ggaaatagcg aaaaggtaaa tgaagcactc ggtcagttgc
      901 gtataaaatt agcacgcaca ctgaatatta ttccggagga agaatataat tttttatggg
      961 ttaccaattt tcccttattt gaatattctc agactgaaaa acgttatagc tctatgcacc
     1021 atccctttac ttctccggta gatgaagata ttccgatgct ggataacaat ccctatcagg
     1081 ctcgttctaa agcttatgat ctggtcttga acggcaatga gatcggggga ggaagtattc
     1141 gtatccatcg caccgaaata cagcagaaga tatttgactt actggggatt aagcatgatg
     1201 aagctgttca aaaatttggc tttcttctgg aagctttgca atttggcgct ccaccacacg
     1261 ggggtattgc cttcggactg gatagaatcg ttatgttact ggcaggcgct tcttctatac
     1321 gtgaggtaat tgcttttcct aaaacgcaaa aagctacctg tcttatgacc ggtgcgcctt
     1381 cagaggttag cccggagcag ttaaaagagt tgcatttaaa aatagactta taattaccaa
     1441 ccattgcatg gttgcagttc atagtttata gtttttggtt tttggaataa tgcaaagatt
     1501 tgggtgtaaa tgtaggcggt cgttggtatt tagtagttag tgaaagaaaa agatgtggga
     1561 ataagtggat gagattgccg cgcctatctt cgataggctt gcaaag
//
LOCUS       DAOQWT010000415         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_94968_length_1603_cov_3.448320, whole genome shotgun sequence.
ACCESSION   DAOQWT010000415 DAOQWT010000000
VERSION     DAOQWT010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94968_length_1603_cov_3.448320"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKJ95_07415"
     CDS             <1..308
                     /locus_tag="PKJ95_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNR66101.1"
                     /translation="DTSTMVTGRPTGHPVRVIKNQLAREFQRLEKINALPEEYERLGK
                     GSLSRAVREGDMEKGSIMAGQIAGLVGKEQSCEEMIKEIFYEAEKIITEKISLLKEK"
     gene            333..1286
                     /gene="fabD"
                     /locus_tag="PKJ95_07420"
     CDS             333..1286
                     /gene="fabD"
                     /locus_tag="PKJ95_07420"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNR66102.1"
                     /translation="MGKIAFLFSGQGSQYVGMGKELFENFSTCREIFRIADETLGLDM
                     GKLCFSGNEEELNQTENTQPAILTTSIAAWKIIEERGFQPDVTAGLSLGEYSSLVCSG
                     ALDFTSAVELVRKRGKFMQEAVPLGVGKMLAILGLTDDKVEKICLEASLEGIVEPANY
                     NYPGQVVIGGENKAVDLAGELAKREGAKRVIPLQVSAPFHTSMLRPASEKLAQELERV
                     EMRDFRIPVISNVIADYIEKKELIKEFLKKQVMYPILWTRCIVRMLQEGVDTFVEIGP
                     GKVLSGFLKKIDTNLIISNVEDLKSLNNTLTILENCVCQGV"
     gene            1271..>1603
                     /locus_tag="PKJ95_07425"
     CDS             1271..>1603
                     /locus_tag="PKJ95_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR66103.1"
                     /translation="MSGSLNGKTAVITGGVRGIGRAIALKLAHSGTSLIVNYNSNKEA
                     AQSLFQEVNSFGGRIVIIQGDVSNYEDARKIIDSALEHYQRLDILVNNAGINRDNLLI
                     RMKEEEFQR"
BASE COUNT          553 a          222 c          399 g          429 t
ORIGIN      
        1 aagataccag taccatggta acaggaagac ctaccggtca tccggtcagg gtaattaaga
       61 atcaactggc aagagaattt caaagattgg aaaaaattaa tgccctacca gaagaatatg
      121 agagattagg gaaaggttcg cttagcagag cagttcggga aggtgatatg gaaaagggtt
      181 ccattatggc cggtcaaata gccggtttag tgggaaagga acagagctgt gaagagatga
      241 ttaaagaaat tttttatgaa gctgaaaaaa taatcactga aaaaatatct ttactgaagg
      301 aaaaataatg ttttatgtag aggtataaaa aaatgggaaa gatagccttt ttattttcgg
      361 gacagggttc acaatatgtt ggaatgggta aagaattatt tgaaaatttc tctacctgtc
      421 gggaaatatt tagaatagct gatgaaactc tcggtctgga tatggggaaa ctgtgttttt
      481 cgggcaatga agaagagctg aatcagacgg aaaataccca gccagcaatc ttaactacca
      541 gtattgctgc ctggaaaata atagaggaaa gaggctttca gccggatgtt acagcaggtt
      601 taagtttggg agaatactct tctttagtct gttcaggtgc gctagatttt acatcagcgg
      661 tagagctggt caggaaacgg gggaaattta tgcaggaagc tgtgccattg ggagttggga
      721 agatgctggc cattctgggg cttacagatg ataaagtgga aaaaatatgt ctggaggcaa
      781 gtctggaggg aatagttgag ccagcaaatt ataattatcc cggacaggtg gtaataggtg
      841 gtgaaaataa ggcagttgac ctggcagggg aattggcaaa aagagaagga gcaaaaagag
      901 tcataccatt acaagttagt gcaccatttc atacttcaat gcttcgaccg gcatcggaaa
      961 aattagctca agaactggaa agggtagaaa tgagggattt tagaattccg gttatcagta
     1021 atgtcattgc tgattacatt gagaaaaaag agttaataaa agaattttta aaaaaacagg
     1081 tcatgtatcc tattctctgg accagatgca tcgtcagaat gctgcaggag ggtgttgata
     1141 cctttgttga gatcgggcct ggtaaagtgt tgagtggttt tttgaaaaaa attgacacaa
     1201 atttgataat cagtaatgtt gaagatttaa agtctctaaa taatacctta actatattgg
     1261 agaattgtgt atgtcaggga gtctaaatgg aaaaactgcc gtaattaccg gaggagtcag
     1321 aggaattggc agagcaattg ccttaaaatt agcccattct ggcaccagtt taatcgtcaa
     1381 ctataattca aataaagagg cggctcagtc cttgtttcaa gaggtaaatt ccttcggcgg
     1441 caggattgta attatccaag gtgatgttag taactatgag gatgcccgaa aaataatcga
     1501 ttctgctctt gaacattatc agagattgga tattttagta aataatgctg gaataaacag
     1561 agataatctt ttaataagaa tgaaggaaga agaatttcaa aga
//
LOCUS       DAOQWT010000416         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_95065_length_1602_cov_3.422107, whole genome shotgun sequence.
ACCESSION   DAOQWT010000416 DAOQWT010000000
VERSION     DAOQWT010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95065_length_1602_cov_3.422107"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PKJ95_07430"
     CDS             <1..527
                     /locus_tag="PKJ95_07430"
                     /inference="COORDINATES: protein motif:HMM:NF023816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66104.1"
                     /translation="HKICKLGMSRTFQITRSFGEMSVEDAIRVGAYNRLGENEVQKKV
                     DEMVEFFELGDIRKTKCSDLGLASLRKVEVARATATEPELLLLDEVGAGLTSVELIKL
                     MAELKKLNQETEVTLCVVEHVMQMVMGLCERIFVLDAGKLIAKGMPDEISNNQKVVEA
                     YLGRRSYIESDVAC"
     gene            514..1215
                     /locus_tag="PKJ95_07435"
     CDS             514..1215
                     /locus_tag="PKJ95_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR66105.1"
                     /translation="MLHVNEISSGYGKITILRDINFQVKENEVVAIIGSNGAGKTTLL
                     KTISNLIPATTGKILFQGKDIVKFPPHMITQLGIAHIPEGGKLFANMTVYENLLMGTY
                     NHRKNFQKSVLEEIYQIFPVLKKREKQLAKTLSGGEKQMLSIGRGLASQPKFIMLDEP
                     SQGIAPKLVDDIYNKLSILKEKGLTILLIEQNINYALHLAERAYVLENGKIVMDGSSE
                     ELLNSEHIKKHYLGL"
BASE COUNT          581 a          210 c          322 g          489 t
ORIGIN      
        1 ctcataagat ttgtaaattg ggtatgtccc ggacttttca aattaccaga tctttcggtg
       61 aaatgtctgt tgaagatgca ataagagtag gggcttataa tcgactgggt gaaaatgagg
      121 tacaaaaaaa agttgatgag atggttgaat tttttgaatt aggagatatc agaaagacaa
      181 aatgttctga tctgggattg gcttccttaa gaaaagtcga agtagccaga gcaactgcaa
      241 ctgagcccga acttttatta ctcgacgaag ttggcgctgg attaaccagt gttgaattaa
      301 taaaacttat ggcagaatta aaaaaattaa atcaggaaac cgaagtaacc ctgtgtgtag
      361 tagagcatgt tatgcagatg gtaatgggtt tgtgtgaacg tatctttgtt ttggatgcag
      421 gtaaacttat tgctaaagga atgcctgatg agataagcaa taatcagaag gttgttgaag
      481 cctatttggg aaggaggagc tatattgaat ccgatgttgc atgttaatga aataagttct
      541 ggttatggca aaataaccat tttaagagat attaattttc aggtaaagga gaatgaggtt
      601 gttgccatta tcggttctaa tggtgctggc aagactacac ttttgaaaac aatttctaat
      661 ttaattccag caacaactgg aaaaatatta tttcagggta aagatatcgt taaatttcct
      721 ccccatatga taactcaatt gggcattgct catataccgg aaggtggtaa actttttgcc
      781 aatatgaccg tctatgaaaa cctgctaatg ggcacctata accacagaaa gaattttcaa
      841 aaatcagttt tagaagaaat ttaccagata tttccggtac ttaaaaaacg agaaaagcaa
      901 ttagcaaaga ctttaagtgg tggagaaaag cagatgcttt ctatagggag aggtctggcg
      961 agtcaaccaa aatttatcat gctggatgaa ccttcccagg gaatagcccc aaaactagtt
     1021 gatgatatat ataacaaatt atctattcta aaagaaaaag gattaaccat tttgttgata
     1081 gaacagaata taaattatgc cttgcatctg gcagaaagag cctatgtttt ggaaaatggt
     1141 aagattgtta tggatggctc cagtgaagaa ttgttaaaca gcgaacacat taagaaacat
     1201 tatttaggat tataaatatt aatatttttt cgattggtaa agagtgtgag ctattaatag
     1261 tcttataatt atttctaaaa gccgtctgtt ttaagatagt taaggtggtt tataaattat
     1321 ttttaaaaaa tttaaattta gtatattata ttggttaaaa aaataattaa ttatatcatt
     1381 tatagattat tatcagaaat atttaataaa aggagacaga aaatagtatg gctgacatta
     1441 tattaattgc tttaggtgga aatgccatta aacaggcaga tgaaaaagga acaacagaag
     1501 aacaatttag aaattgttgg aaaactacag aacatatcgc caatattatc cagaatttaa
     1561 cttctgagga ccgtttatta ataacccatg gcaatggacc cc
//
LOCUS       DAOQWT010000417         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_95329_length_1600_cov_1.587055, whole genome shotgun sequence.
ACCESSION   DAOQWT010000417 DAOQWT010000000
VERSION     DAOQWT010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95329_length_1600_cov_1.587055"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PKJ95_07440"
     CDS             <1..251
                     /locus_tag="PKJ95_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66106.1"
                     /translation="SVISPILAGFLNGGDRKLYLFKNKYSVSDHWLTHTAKTDVVVGI
                     TRTIVQIECQNTSVRSIAPVATAEKSVYLFKTLHFKNG"
     gene            complement(248..583)
                     /locus_tag="PKJ95_07445"
     CDS             complement(248..583)
                     /locus_tag="PKJ95_07445"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR66107.1"
                     /translation="MIDNLLVFQKTYDWLLWIKSATERFSRVHKYSLGQQLQIESINL
                     LKKIIIANQQKNKFAAINECLTQKEIVKILVRLSKDYSLFDNRQYEFAAQRLLEIDRM
                     LLGWQRISN"
     gene            complement(645..1061)
                     /locus_tag="PKJ95_07450"
     CDS             complement(645..1061)
                     /locus_tag="PKJ95_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66108.1"
                     /translation="MDGTDSLTDIHKRTLTLSNGEVIWDIAGNVWEWTDATILGKDEP
                     QGVANPTGFGWYQYSNLYSYGALSPERLLPLNPTWNTNQGYGRIYTSGVPTNMTTYAF
                     LRGGYRSNGTDAGVLALALGYGPGLSDSNVGFRCVR"
     gene            complement(1111..>1600)
                     /locus_tag="PKJ95_07455"
     CDS             complement(1111..>1600)
                     /locus_tag="PKJ95_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66109.1"
                     /translation="EMYYADVGAYPLPPTSTGIIGGLCLSDLGISSTCGVNTYLAKIP
                     DDPKGSKHYLYSTVVNPQYYELIYEEETIPDDCPPYWVRVPGNTLYHTRTFCVMQFEA
                     KIKGQDDGNQTYSASYEPESRPGGTPWVNISQINAKSECQSIGAHLITEDEWPTGQLD
                     PG"
BASE COUNT          504 a          340 c          307 g          449 t
ORIGIN      
        1 aaagcgttat ttcgccaatt ttggcaggct tcttaaatgg gggtgaccga aaactctatc
       61 tatttaaaaa taaatattct gtttcggacc actggctaac gcacacagcg aaaaccgatg
      121 ttgttgttgg aattacccgg accattgttc aaattgagtg tcaaaacacc agcgttcgtt
      181 ccattgctcc agttgccacc gcggagaaaa gcgtttattt atttaagacc ctccatttta
      241 aaaatggcta attagaaatt cgctgccatc ccaacaacat tcgatctatt tccaacaaac
      301 gctgagctgc aaattcatac tgacgattat caaacaaaga ataatcttta gataacctaa
      361 ctaaaatttt tactatttct ttttgcgtta aacattcgtt aattgccgca aacttatttt
      421 tttgttgatt ggcaattatt attttcttta ataaatttat tgattcaatc tggagttgtt
      481 gtcccaaact atatttatgc actcggctga atctttcggt cgctgactta atccacagca
      541 accaatcata agttttttga aaaactaaca aattatcaat cattttttta ttttaatact
      601 aaaatttaaa tacagatttt agataaaaga taagacaccc ctggctaacg cacacagcga
      661 aaaccgacat tgctgtcgga aagacccgga ccatagccca aagcgagtgc caaaacacca
      721 gcgtccgtac cattgctccg gtagccaccg cggagaaaag cgtaggtagt catattggta
      781 ggaacgccac tagtatagat gcggccataa ccctggttag tattccaggt aggatttaag
      841 ggcaaaagac gttcaggaga aagagcgcca taagaataaa ggttcgaata ctgataccat
      901 ccaaaaccag taggattggc cacgccctgg ggctcatctt tacctaaaat agtcgcatca
      961 gtccattccc agacattgcc agctatatcc cagataactt caccattaga taaagtaagg
     1021 gtacgtttat ggatatcagt aagagaatca gtaccatcca tagccgcact gctatcgcta
     1081 ttacctcgag gaatttgacc agaaccaacg ctacccaggg tccaattggc cggttggcca
     1141 ttcatcttca gttataaggt gagctccaat agactgacat tcacttttag cattgatttg
     1201 agagatattg acccaagggg taccaccggg tcgagattca ggttcatagc tagcactata
     1261 ggtttggtta ccatcatctt gacctttgat tttagcttca aactgcatca cacaaaaggt
     1321 acgggtatga tataaagtat tgcctggtac tctaacccag taagggggac aatcatcagg
     1381 gatggtttct tcctcataga ttagttcata gtattgaggg ttaactacag tagaataaag
     1441 ataatgttta ctgcctttag gatcatcagg gattttagca agatacgtat taactccgca
     1501 ggtagaagaa ataccgaggt cactaagaca aagaccgcca ataatcccag tagaggtagg
     1561 tggtaaggga taggcgccaa catcagcata atacatctct
//
LOCUS       DAOQWT010000418         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_95827_length_1594_cov_3.534113, whole genome shotgun sequence.
ACCESSION   DAOQWT010000418 DAOQWT010000000
VERSION     DAOQWT010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95827_length_1594_cov_3.534113"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(472..1350)
                     /locus_tag="PKJ95_07460"
     CDS             complement(472..1350)
                     /locus_tag="PKJ95_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNR66110.1"
                     /translation="MKKNYIYGYVKESIRQEDLIYQLDILKGYKCKEIITEKTSATKA
                     ERPELDRLKDKVKSGDTIVIESFSRLGRSTKDLIELVDYFVNNGVNLISIKENFDTST
                     DMGKIMLSTFQAFSQFERDLITRRTREGLINARAIGRKGGRPRVKNNNVEKALELYNS
                     KAYSISEIVEISGISQATLYRYKKAEAQDNKPDAEIEKTAKINMYLRVENNSKFVRGK
                     KKVRENIERYLINHYNMRKLTNNNWDYTLYVKYKTIDELTKEVYDILRELSDEAEWRN
                     CFIEDDTNCEELGLSW"
BASE COUNT          444 a          289 c          235 g          626 t
ORIGIN      
        1 taacaggttt taagaaactc gttaaaacgc aaaatagagc tgttttgcaa tgcagtattt
       61 taggtaaaat gcgatattta gcacttgcaa tatggcctgt agtaatgatt ttcattaatt
      121 caggttacca ggtagattta ctcaaaaagg ataggttttt ataaaaaata ttcctggata
      181 gaatattttt tcgagggaaa cagggagaat gtttctcagg atttgcccat cccctcgaaa
      241 agtacaatag aggatcaaca gttattgtat tgtggctgtt ttttgcacga atcgtttcct
      301 taccccttat tgagaggata atcttctaat aagaatttaa attatataaa cctggctgaa
      361 ggtgttttat gactggcttc tctgtaatgt atgtctgaat gatatttcct ggcaaaaagt
      421 aaagctttac tcacatgcat tggtttttag tagaaaaaaa taacaatatt attaccagct
      481 taatccaagt tcttcacaat ttgtgtcatc ctcaataaag cagttacgcc actcagcttc
      541 atcacttaat tcacggagta tgtcataaac ttcttttgtt aactcatcaa tagttttata
      601 tttgacatag agagtatagt cccagttgtt atttgttaac tttctcatat tataatgatt
      661 tattaaatat ctctctatat tttctcttac tttcttctta ccacgaacga atttactgtt
      721 attttcaact ctgagataca tatttatttt agctgtcttc tctatttcag catcaggttt
      781 attatcctga gcttctgcct ttttatatct atataatgta gcctggctta ttccagatat
      841 ttctacaatc tcacttatac tgtaagcttt tgaattatac agttccagag ctttttcaac
      901 attattgttt ttcaccctgg gtctaccgcc ttttcttcct atggctcttg catttatcag
      961 tccttccctg gttctccgag tgataagatc tctttcaaat tgactgaatg cctggaatgt
     1021 agagagcatt attttcccca tatctgtgct tgtgtcaaag ttttccttga tacttatcag
     1081 attaactcca ttattcacaa aataatcaac cagttctatc aggtcttttg tgcttcttcc
     1141 caatcttgaa aagctttcta taactattgt atcaccggat tttactttat cttttaacct
     1201 gtccagttct ggtctttcag cttttgtagc tgatgttttt tccgttatga tttccttgca
     1261 tttataacct ttaaggatat ccagttgata gattaaatct tcctgtctga tactttcttt
     1321 tacatatcca tagatataat tctttttcat ttttacacct tccgttcctg ataatattat
     1381 cataaagggt agtttttgta aaattctttt tgacagtaat tatgataata gatttgccca
     1441 tcccctcgaa aaggccggta gtaagagtaa taaggtattt caggtaattc ggtctaaaca
     1501 gggggaatgt ttctttgaat ttcttcattg aatattctta ccttcttcat agcttcttca
     1561 catctttaag ttatacttac ctcaatgaaa gaga
//
LOCUS       DAOQWT010000419         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_96043_length_1592_cov_2.602472, whole genome shotgun sequence.
ACCESSION   DAOQWT010000419 DAOQWT010000000
VERSION     DAOQWT010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96043_length_1592_cov_2.602472"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PKJ95_07465"
     CDS             complement(<1..258)
                     /locus_tag="PKJ95_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66111.1"
                     /translation="MRFSGRDKSTLGLIPVVLFLLLWEIIARKNIFPGNFYFPPFSVV
                     MTEFYYLTVNGILGKNFFSSLYRVLIGFSLGTIAGLVVGILI"
     gene            complement(255..1277)
                     /locus_tag="PKJ95_07470"
     CDS             complement(255..1277)
                     /locus_tag="PKJ95_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR66112.1"
                     /translation="MRKLILFVLLVSTILFILNGVFIADICSSEEIPIRVAVEFMDHA
                     AAAYISQAQGWFEEEGLNINYYENYVTGMALAAALGRGDIQAAYLCLIPAINAYANAR
                     VAIKIVAGTHKYGYGLVVNTNKIKDIYGLQREGIRIGCVREGGAVDVLLHKAIIEYGL
                     DKEKVLNNVQRMDPAKQLLAIQMDRLDAAFLPEQWATMAEDSKYQMMLMSQDIWPEMQ
                     GSVLVVKSELIEKYPELVRKLVKVTEKSTDWIIQNPESAAEVVAHQLSLVGENILPVQ
                     VKDADTDKIEFNADVLLKSMSRIDYGTDIDSVTVQDNIDFLVELGYIKNTFDANEILD
                     LRFLSR"
     gene            complement(1382..>1592)
                     /locus_tag="PKJ95_07475"
     CDS             complement(1382..>1592)
                     /locus_tag="PKJ95_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNR66113.1"
                     /translation="IRKNGITIEDVSHTEEIIAWSDLIIATGTTAVNGTLVPLLNKKP
                     IIFYGVTISGIAYLMGYEQLCFCGH"
BASE COUNT          494 a          351 c          230 g          517 t
ORIGIN      
        1 aattaaaatg ccaactacca gtccggcaat ggtacctaaa gaaaacccga taagaacacg
       61 gtaaagactg ctaaaaaaat tttttcccag tataccatta acagtgagat agtaaaattc
      121 agtcatgacc acagaaaaag gggggaaata aaagttgcca ggaaatatat tttttctagc
      181 aatgatttcc cataataata agaaaagcac tactggaata agtcctaaag ttgatttgtc
      241 tcttcctgaa aatctcatct actcagaaac ctcaaatcta atatctcgtt ggcatcaaag
      301 gtgtttttta tataacctaa ttccacaaga aaatctatat tatcctgaac agttacagaa
      361 tcaatatcag taccataatc tattctactc atagatttaa gtaaaacatc agcattaaat
      421 tctattttat cagtatctgc atcttttacc tgaacaggta gaatattttc gccaactaga
      481 gataattgat gtgcaactac ctcagcagca ctttctggat tttgtataat ccaatcggtt
      541 gatttttctg ttactttaac taactttctc accagctcgg ggtatttctc aattaattct
      601 gatttgacta ccaggacact gccctgcatt tccggccaga tatcctgact catcaacatc
      661 atctggtatt ttgaatcttc cgccatggta gcccactgtt cgggtaaaaa agctgcatct
      721 aatctatcca tctgaatagc taagagctgt ttagccggat ccattctttg aacattattt
      781 aatacttttt ctttatcaag cccgtattca attattgcct tatggaggag gacatctacc
      841 gccccacctt ccctaacaca cccaattctt attccttctc tttgtaatcc ataaatgtct
      901 ttaatcttat tagtattaac taccagacca tatccatact tatgtgttcc tgccactatc
      961 tttattgcta ctctggcatt ggcgtaggca ttgatagccg gtattaaaca gaggtaagct
     1021 gcttgaatat caccacgccc cagagcagca gccagagcca ttccagtgac ataattttca
     1081 tagtaattga tattcaaacc ctcttcttca aaccatccct gtgcctgact gatataagcc
     1141 gcagcagcat ggtccataaa ttctaccgct acccttatag gtatctcctc actactgcag
     1201 atatctgcta taaaaacccc atttaaaatg aataggattg tcgacactaa taaaacgaat
     1261 aaaattaatt ttctcattat ttaccagcct cttattgcta tatttattat ttcctatgta
     1321 ttactctgtt atcatgatgt tttgtgacgt tcctatagaa cctgacctgc ctcttctatt
     1381 ttcaatgacc acaaaaacag agttgttcat aacccataag ataggcaata ccactgatag
     1441 ttacaccata aaaaataatg ggttttttat ttaagagtgg taccagcgta ccattaactg
     1501 cggtagtccc ggttgctata ataaggtctg accatgctat tatctcttcg gtatgtgaaa
     1561 catcttctat ggtaatacca tttttcctta ta
//
LOCUS       DAOQWT010000420         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_96935_length_1583_cov_3.541885, whole genome shotgun sequence.
ACCESSION   DAOQWT010000420 DAOQWT010000000
VERSION     DAOQWT010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96935_length_1583_cov_3.541885"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="PKJ95_07480"
     CDS             <1..1053
                     /locus_tag="PKJ95_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNR66114.1"
                     /translation="KPDSVCVELCQSRYQSITDPEKWKNMDIITIIKQKKALLLLVNL
                     ILSAFQKRIAHQLGLNPGQEMIEAIQVAKENNINLVLADRDIQLTFTRIWKKLGFFGK
                     FKLLFMLLMSIFAKEDISEEEIERLKSEDVLTAALNDLAVSFPRLKSTLIDERDQYLA
                     EKIKNAPGKKIVAVVGAGHIPGIKEELFKEHDLNALNYIPKSANWVKWLLWAIPIAII
                     VMIISTFRISAISGMNQILQWLLWNGSLAALGAFLTAAHPLSILTAFLAAPITSLNPL
                     LAAGWFAGITEAIVRKPRVEDFENLGNITTFGDFFRNRVTKILLVVALTNLGSSFGTF
                     IGGAKIIQIFMNTISS"
     gene            complement(1173..>1583)
                     /locus_tag="PKJ95_07485"
     CDS             complement(1173..>1583)
                     /locus_tag="PKJ95_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNR66115.1"
                     /translation="MRSLFDEYFDVMGRPEWHRRRRWEEGGVWSPAIDVLDQKDKLVA
                     RVELPGVDQKDIKVSLTDSNLTIEGEIEKDKDVKKEDYYCCERVYGKFSRSISLPAGI
                     DKDNIKAKYKNGILEISLPKKPEEKPKEITIETN"
BASE COUNT          451 a          326 c          293 g          513 t
ORIGIN      
        1 aaaccagatt ctgtatgtgt ggagttgtgt cagtcacgat accagtccat tactgacccc
       61 gaaaaatgga aaaatatgga cattattacc attatcaaac agaaaaaggc tcttctctta
      121 ttggtaaatc tgatactatc tgcctttcaa aaaaggattg ctcatcagct tggactaaat
      181 ccgggacagg aaatgattga ggctatacag gtagctaaag aaaataatat caatcttgtt
      241 ctggctgatc gtgatataca gctcactttt accagaatat ggaaaaaact gggatttttc
      301 ggtaaattca aactattgtt tatgctctta atgagtatct ttgccaaaga ggatatttca
      361 gaagaggaga tcgagaggct aaaatccgag gatgtactta ccgccgccct gaacgatctg
      421 gctgtatctt ttcctcgctt gaagagcacc ttaatcgatg aacgtgacca gtatctggca
      481 gagaaaataa aaaatgctcc gggtaaaaag atagttgccg tagtcggcgc aggacatata
      541 ccgggaatta aagaagaatt atttaaagaa catgatttaa atgccttaaa ttatattcct
      601 aaaagtgcta actgggtaaa atggctcttg tgggctatcc ccattgccat tatcgtaatg
      661 attatttcca cattcagaat atctgccatc agcggaatga atcaaatctt acaatggcta
      721 ctctggaatg gctctctggc tgccctgggt gctttcctta ctgctgccca tccactgtcc
      781 atcctgaccg cttttttagc ggctcccatc acctcgttaa atccgttgct ggcagccggt
      841 tggtttgccg gtataacaga ggcaatagtt agaaaaccaa gggtggaaga ttttgaaaac
      901 ttaggcaata tcacgacatt tggtgatttt tttcgaaatc gggtaactaa aatactgctg
      961 gtagtagcat tgaccaatct gggcagttcc ttcggcacat tcataggcgg ggcaaaaatt
     1021 atccagatat ttatgaatac aatttcttct taatctttca gctctatttt taataatgtg
     1081 cttgtgggaa gataaccacc ctatcctaga tatattgtgt ttaaataaaa aaggggaata
     1141 taaattcctc tttttttctg ctacacatct tattagtttg tttcgatggt tatttctttg
     1201 ggtttttctt ccggtttttt aggtaatgaa atctccagaa ttccattttt gtattttgcc
     1261 ttaatattgt ctttatctat tcctgccggc agggaaatac ttctggaaaa cttaccataa
     1321 accctttcac aacaatagta gtcttctttt ttaacatctt tgtctttttc tatttcacct
     1381 tctatagtaa gattactatc ggttagcgaa actttgatgt ctttctggtc aacccctggc
     1441 aattcaaccc tggccactaa tttatccttc tggtcgagca catcgatggc aggagaccat
     1501 actcctcctt cttcccatct tctccttcta tgccattccg gtcttcccat tacatcaaag
     1561 tattcatcaa aaagactgcg cat
//
LOCUS       DAOQWT010000421         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_98498_length_1568_cov_3.087244, whole genome shotgun sequence.
ACCESSION   DAOQWT010000421 DAOQWT010000000
VERSION     DAOQWT010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98498_length_1568_cov_3.087244"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PKJ95_07490"
     CDS             complement(<1..874)
                     /locus_tag="PKJ95_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR66116.1"
                     /translation="MILQGLKIALESLWANKMRSFLSMLGIVIGVGAVIAIVSVGTGS
                     TQQITARIANLGSNMITIYPRALRGQFGRLSSISSQQKFTLELSEHLEEFCPSVKQVI
                     PLKQVNGRLISDDVNINSAIIGTRAEYTEINNYEIAQGVFINQEDDLERRQVIVLGHQ
                     LASDLFDNENPLGNLVRLNYDGRIFQFTVIGVMKEKGGSMAGNLDERAYVPLSVVMDR
                     LTAQKYVDSLLAQAYSSDEANRAVQEIEYFMQKHLEDEKREQFRITSQDEILDTVSSV
                     TNTMNMMLGGIAAIS"
     gene            complement(871..1557)
                     /locus_tag="PKJ95_07495"
     CDS             complement(871..1557)
                     /locus_tag="PKJ95_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR66117.1"
                     /translation="MSIVIRVEQLKKVYHLGKVKVEALRGISFQVEEGEFVSIMGPSG
                     SGKSTLMHIIGCLDYPSEGKYFLTNHDVTKLDDNQLAIFRNKKIGFVFQQFNLLPRAT
                     NLRNVEIPLIYAGMPFRERQRQTLKALQDVGINHRAHHHPNEISGGEKQRVAIARALV
                     NNPSIILADEPTGNLDSKTGLEIMKIFQNLNQKGNTIILVTHEPELAHYAGRIIHLRD
                     GIINREEVLA"
BASE COUNT          446 a          347 c          258 g          517 t
ORIGIN      
        1 gagagattgc agctatacct cccaacatca tgttcattgt attggtaacc gaacttacgg
       61 tatccaatat ttcatcctgg ctggtgattc tgaattgctc tcttttttca tcttccagat
      121 gtttttgcat aaaatattca atttcctgga cagctctgtt tgcctcatca gaggaataag
      181 cttgtgctaa cagtgagtca acatattttt gtgctgtcag ccgatccata acaacagata
      241 ggggaacata ggcacgctca tcaaggttac cggccataga accacctttt tctttcatca
      301 ctccgattac agtaaattga aatattcttc catcataatt aagtctgacc aaattgccta
      361 atgggttttc attatcaaat agatcggatg ccagttgatg acctaagact attacctgtc
      421 ttctttccag gtcatcttct tggttaataa atactccctg agcaatttca tagttattta
      481 tttcagtata ttctgccctg gttcctatta tggcactatt aatatttaca tcatcagaaa
      541 tcaacctgcc attgacctgt tttagaggaa ttacctgttt tacagaaggg caaaattcct
      601 ccagatgttc tgataactct aaagtaaatt tttgttgaga acttattgaa ctcaaccttc
      661 caaattgacc cctgagggct cttgggtaaa tggtaatcat atttgaacct aaattagcaa
      721 tccgggcagt tatctgttgt gtggaccccg ttcctaccga aacaatagca attacagcac
      781 ctacacctat cacaatcccc agcatagata agaagctacg cattttatta gcccataaac
      841 tctccagggc tatctttaat ccctgtaaaa tcatgccagt acctcttctc tattgataat
      901 tccatccctg agatgtatta tccttccggc ataatgagct aattccggtt catgggtaac
      961 cagaataatt gtattgcctt tttgattcaa gttctgaaaa attttcatta tctccagacc
     1021 ggttttagaa tcaagattac cggttggctc atctgccagg ataatagaag gattgttaac
     1081 taaagctctg gcaatggcta ctctttgctt ctcgccacct gaaatctcat tggggtgatg
     1141 gtgagcccga tggtttatgc ctacatcctg gagggctttt aaagtctgtc tttgcctttc
     1201 tctaaatggc ataccggcat agattagggg aatctccaca tttcttagat tggtagctct
     1261 gggtaataga ttaaattgct gaaagacgaa acctatcttt ttatttcgaa aaatagctaa
     1321 ttggttatca tccaatttag ttacatcatg attagtaaga aaatatttcc cttcactggg
     1381 gtaatccaga caacctataa tatgcatcaa ggttgacttt cctgaaccag aaggacccat
     1441 aatagagaca aattctcctt cctcaacttg aaaagaaata cctctcaatg cttccacctt
     1501 caccttaccc agatgataga ccttttttag ttgttcaact cttattacaa tactcatctt
     1561 cacctcat
//
LOCUS       DAOQWT010000422         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_98718_length_1566_cov_2.892124, whole genome shotgun sequence.
ACCESSION   DAOQWT010000422 DAOQWT010000000
VERSION     DAOQWT010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98718_length_1566_cov_2.892124"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKJ95_07500"
     CDS             complement(<1..211)
                     /locus_tag="PKJ95_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HNR66118.1"
                     /translation="MELNELKEMKISELCKIANEYEINNCARMKKTDLIFNILKAQTE
                     KQGYMFSEGILDIMKAGWQVVARTGA"
     gene            complement(538..1308)
                     /locus_tag="PKJ95_07505"
     CDS             complement(538..1308)
                     /locus_tag="PKJ95_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNR66119.1"
                     /translation="MNALILAGKKDTGKLENCTKLSNKALLMFNEIPMVEYVVNALLE
                     SKLINDIVVVGPEYDLTPFLEKKVTKILESTDSIIDNVQIGLDYLNDDGKILLLTSDI
                     PLITGEIIDRFIIKCEQHDAFFYYPITTRETILKKYPETVRSYFVLKEGIFCGGNMVI
                     FNSPVFDRNRELADELYQNRKDVKKYIRVLGMKFLAKYLLKALTIKEIEEKAAQIIGY
                     PVKSITIDDPEVMIDLDKLSDYELILQAINQKTPNSSS"
     gene            complement(1347..>1566)
                     /locus_tag="PKJ95_07510"
     CDS             complement(1347..>1566)
                     /locus_tag="PKJ95_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNR66120.1"
                     /translation="FNNRYLSKFEQSDIMFSGVNLENNLVEIFELKNHPWFIGTQFHP
                     EFKSRPNRPHPLFKSFLEAAIKMNSSEV"
BASE COUNT          498 a          289 c          219 g          560 t
ORIGIN      
        1 ttgcaccagt acgcgccacg acctgccatc ctgctttcat aatatccaaa ataccctcag
       61 aaaacatata accctgtttt tcggtctgag cttttaagat attaaaaatc aaatctgtct
      121 ttttcatacg agcacaatta ttgatttcat actcattggc gattttgcac agttcgctta
      181 ttttcatttc ttttaattca tttaattcca aagtataata ccccctaagg tttttctatc
      241 ttttaatatt taaataataa ataaggcact cacctggcag gtggtttaca ggactgtttt
      301 atttaaaaaa gctgcaatta gcaaagaaaa tactaatttc attgaactaa taggcaaaca
      361 gtgggaaaaa ttcctggaga ataacctggt agtcgcactt ttttaaaatt atatcacttc
      421 ttttttcaaa aacaatatat tttgtaaaaa tctaaagaat atctttctca ataaaatcat
      481 ctcaatctta aatcttcatt tcattatgaa taacaaaaaa ttctgttata agttatgcta
      541 tgatgaggaa ttaggggttt tttgattaat ggcttgtaat atcaattcat aatcactcag
      601 cttatcaaga tcaatcataa cttccgggtc atcaatagtg atacttttca ccgggtagcc
      661 aattatctgg gctgccttct cttctatttc ctttatagtt aacgctttca gcaaatactt
      721 tgctaaaaat ttcattccca gtactctaat atatttctta acatcttttc gattctgata
      781 aagttcatct gctaactctc tgttcctatc aaagacaggg ctgttaaata ttaccatatt
      841 accaccacag aatattccct ctttcaatac aaaataagaa cgaacagttt caggatattt
      901 tttcaggatg gtttccctgg tagtaatggg ataataaaaa aaggcatcgt gctgttcaca
      961 ttttattata aaacggtcta taatctctcc ggtaatcaat gggatatctg aggtcagtaa
     1021 taatatttta ccatcatcat ttaaataatc aagcccaatc tggacattat ctataatgga
     1081 gtccgtggat tccagaattt ttgtcacttt tttctccaga aagggggtca aatcatactc
     1141 tggtccaact accacaatgt catttatcaa ttttgattct aagagggcat tgaccacata
     1201 ttcgaccatg gggatttcgt tgaacattaa tagtgcttta ttactaagtt tggtacagtt
     1261 ttctaatttc ccggtatcct tttttccagc caggattaac gcattcattt tgaccttact
     1321 ccacctttaa ttgaggtctg ttttcattat acctcagaag aattcatttt tatggcagct
     1381 tcgagaaaac ttttaaataa tgggtgtggc cgatttggtc tagacttaaa ttcaggatga
     1441 aactgagttc caataaacca aggatgattt tttaattcaa aaatttcaac aagattattt
     1501 tctaaattaa ctccactgaa cattatatca gattgctcaa attttgacaa atagcggtta
     1561 ttaaat
//
LOCUS       DAOQWT010000423         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_98917_length_1564_cov_3.041087, whole genome shotgun sequence.
ACCESSION   DAOQWT010000423 DAOQWT010000000
VERSION     DAOQWT010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98917_length_1564_cov_3.041087"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            634..>1564
                     /locus_tag="PKJ95_07515"
     CDS             634..>1564
                     /locus_tag="PKJ95_07515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR66121.1"
                     /translation="MVKNAELAEVFADIARILKIKDENVFKIRAYERVADTLENLPME
                     AETLYRLGKLNEIPGVGEAIAQKIGELVETGRLEYYEKLKKSIPAGVLELLNVPDIGP
                     RKAKLFYEELGISSVEQLEKAALQHKLQKLSGMGKKAEENILRGIELYKRREQRFLLG
                     TALPIAEEIVSEFKSLGEVEKISIAGSLRRRKDTIGDIDLLIASENPGKIMDKFIHLP
                     QAKEVLAEGLTKSAILTHKDMQVDLRVVSSDSFGAALLYFTGSQSHNVHLREMAVKQG
                     LKINEYGVFQTKDDKKIAGEEEEEIYRILGLPYI"
BASE COUNT          571 a          195 c          304 g          494 t
ORIGIN      
        1 tcccttttcc attcatctaa catctcctta gtgaatggtt tagaatactg gaaactataa
       61 ataaataaga agacatgtaa taatctttac acccattaat tatgaacaca aaattaacac
      121 atagctcaaa atcttacgac agatttctgt ttcaattaca aatttttttc tttttctgat
      181 caaacagatg cttcaaagga aagtgaaggc ctaaaggctt tactattata ttcttaaagg
      241 taaccatatt taatttttat ctttcaaagt tatttattgc tctctttttt atttagacaa
      301 gataaatctt tgtaatatat tatacaatat ttattattca acagtaacat ttttggtaaa
      361 atatgtttgt agtattagat tgttctatca ggagaattaa ggattactat tctattttta
      421 tatgtgctat tttatcttgc agactagtct actatttata agtattatat catattatat
      481 cgtacaattt ataaattata tatagcaatt aatactggta aagaaataga atacaagata
      541 aaaagatatg tgggtattat tttatatttt ctttgtgata tgataatagg gaactataaa
      601 atagattttg aatcaataaa gagaagatta attatggtta aaaatgcaga attagctgaa
      661 gtttttgccg atattgccag gatactaaaa ataaaagatg aaaatgtgtt taaaataagg
      721 gcatatgaaa gagtagcaga tactctggag aatttaccta tggaggctga gactctctac
      781 cgattgggga aattaaatga gattccagga gtaggagagg cgattgccca gaaaattgga
      841 gaattagttg aaacaggtcg acttgaatat tatgagaagt taaaaaagag cattccagct
      901 ggggttttgg aattattaaa tgttcctgac atagggccac gcaaggcaaa gcttttttat
      961 gaggaattgg ggattagcag tgtggaacaa ttagaaaagg cagcgcttca gcacaaattg
     1021 cagaaactgt caggaatggg taaaaaggca gaagagaaca ttttaagagg tatagagtta
     1081 tataaaagga gagagcagag attccttctg ggaactgctt taccaattgc tgaggaaata
     1141 gtcagtgaat ttaaatcact tggggaagtt gaaaagatta gtattgccgg cagtttaagg
     1201 cgaagaaagg atactattgg agatatagat ttactgattg cttcagaaaa cccgggaaaa
     1261 ataatggata agtttatcca tttgccgcag gcaaaagaag ttctggcaga aggcttgacc
     1321 aaatcagcaa tattaacaca caaagatatg caggtagact taagagtagt atcctctgat
     1381 agttttggtg ctgctttatt atactttacc ggttctcagt ctcataatgt tcatctgaga
     1441 gagatggcag tgaagcaagg tttaaaaatc aacgaatatg gagtatttca aacgaaagat
     1501 gataaaaaga ttgccggtga agaggaagaa gaaatctata gaatactggg attaccatat
     1561 atta
//
LOCUS       DAOQWT010000424         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_99468_length_1559_cov_2.884309, whole genome shotgun sequence.
ACCESSION   DAOQWT010000424 DAOQWT010000000
VERSION     DAOQWT010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99468_length_1559_cov_2.884309"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="PKJ95_07520"
     CDS             complement(<1..1523)
                     /locus_tag="PKJ95_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66122.1"
                     /translation="MKKITKSLSFAIFTIVIILAICPKSFANLEVREYLEGKFPAIFI
                     IYLSSLEDLDEYEKEFIDLLEKTPAAEQRIFAREVYEEGFSLELLERLKLREQPKRVV
                     KPFLKVAYPGQDKQTIRGNPIFVFGCTYPSPEVKVTVNGVEAERFDYRTGNFLTLVEV
                     PEGMEFPIKVTANINGEEVSLERTVRYEPFWKEMPSTPLALHPISVQPRQDQILRKGD
                     LLKVNIQGSPAAEAVFRIGNNEKEIKMEEIKSLITSFSGKGIYQGSYLIKDSDVPSTG
                     KTAPQVITIILRRGSEQISRELPGKVTFSSGNPPQVVEVGGERTRFYRIIDDSPVLPG
                     STLGGGSWLTQVINFDLLPETRFRISGRAGEYWRFKLRTENYLIHQENVQEMPVERIK
                     TNPTLSEITLREDKRGTEIRFHNYKHSPFIIEDEPRQLKLILYEVKYSENVIIRGESS
                     SVKAIEITPYPEEQLSGVIEISIKLNHLLAGFNYYWDGSDLVISLRKLPDILKSNPL"
BASE COUNT          436 a          336 c          249 g          538 t
ORIGIN      
        1 tgaagaggat tactttttaa gatatcaggc agctttctta aacttattac taaatctgaa
       61 ccgtcccaat aataattgaa tccagccaat agatgattca gtttaatact tatttctatg
      121 acaccggaca attgttcttc agggtaagga gtgatctcaa tagccttaac agaggaggat
      181 tcccctctta taataacatt ttcactatat ttcacctcat ataaaattag tttcagttgc
      241 cggggttcat cttctatgat aaagggcgaa tgtttataat tatgaaaacg aatctccgtt
      301 cctcttttat cctctcttaa ggttatctca gaaagggtag gattagtttt tattctctcc
      361 acaggcatct cctgtacatt ttcctggtga attaaataat tttctgttct caatttgaac
      421 cgccagtatt ctcctgctct tcctgaaatt ctaaaacggg tctccggtaa aagatcaaaa
      481 tttattacct gagtaagcca actacctccc ccgagggtag agccgggaag gacaggagaa
      541 tcatcaataa ttctataaaa cctggttctc tcaccaccca cctctaccac ctggggtgga
      601 tttccagaag aaaaggtcac ctttccaggt aactccctgc ttatctgttc acttcctctg
      661 cgtaaaatta tagttattac ctgcggagct gtctttccgg tagaaggtac atctgaatct
      721 tttataagat aacttccctg ataaatacct tttcctgaaa aagatgtaat taaagatttt
      781 atttcctcca tctttatctc tttctcatta tttccaatcc gaaacactgc ctcagctgcg
      841 ggacttccct gaatatttac ttttaatagg tctccctttc tgagtatttg atcctgtctg
      901 ggctgtacac taatgggatg taaagccagt ggcgtagaag gcatttcttt ccaaaaaggc
      961 tcatacctca ctgttctttc cagggaaact tcctccccat ttatatttgc ggtaacttta
     1021 ataggaaatt ccattccttc cggcacctct accagagtaa ggaaattacc tgtgcggtaa
     1081 tcaaatctct ctgcctctac tccgttaaca gtgaccttta cctctggaga gggataggta
     1141 cagccaaaga caaaaatagg gtttccccga attgtctgtt tatcctgtcc gggataagcc
     1201 acttttaaga aaggttttac tactcttttc ggctgttctc ttaatttcaa tctttccaac
     1261 aattctaaag aaaaaccctc ttcatatact tctctggcaa atattctttg ctcagctgcc
     1321 ggtgtttttt ccagtaaatc gataaattct ttttcatact catctaaatc ttccagagag
     1381 gatagatata ttataaagat tgctgggaat tttccttcca gatattctct tacctctaaa
     1441 tttgcaaaag atttggggca tattgccagg attatcacta tggtaaatat tgcgaatgat
     1501 agagattttg ttattttttt catattataa ctcatttata ctttaatata cattttatt
//
LOCUS       DAOQWT010000425         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_99862_length_1555_cov_3.325333, whole genome shotgun sequence.
ACCESSION   DAOQWT010000425 DAOQWT010000000
VERSION     DAOQWT010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99862_length_1555_cov_3.325333"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="PKJ95_07525"
     CDS             complement(<1..1390)
                     /locus_tag="PKJ95_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNR66123.1"
                     /translation="MNSIDTVNDNNTINSEGSIKNVFKEKLGEFTSLDINKINISEFN
                     TRCQFLDHLHVKTLMASIEKRGYIPKSAVWVNAVTDNGTKQGNIIQYRLVAGRHRYEA
                     CRRIELNEIPSQLYYNLTDEEECELDTIDNELDEHHKPINFLEVAEHYKYLKDIKGWS
                     IRQIARAKNVSKSDVARKLNITELSNEVKQILKSVPPGGQLHEKYFRDICKLTSIPHQ
                     ILVCNEIIGEQTDIDVGTSQFQDKKSEVNLKCTPPQKTMTQSDVELRVNQLLALELQG
                     KIAREAIPFLNDKAELEDLLSASSDEYGNNSNGTGEPITGQVFFEDYYSVNDTSGIDT
                     KNKYSKQSQKIPGSNKLVEKNQEEPKAEQLLFDIFATMSDDREEKGVRFGVKPLWIKH
                     SGLVRELTSGAYLLLLELIEDDFRYKPKKNNFFFIKFDEQYANCIDFLSYVTGVKPVT
                     LVKKILPELEKFV"
BASE COUNT          446 a          302 c          227 g          580 t
ORIGIN      
        1 aaacaaattt ctccagttcc ggtaatatct tttttaccag tgtaacaggc tttacgccgg
       61 taacataaga aagaaaatca atgcaatttg catattgctc gtcaaactta ataaaaaaga
      121 aattattttt tttcggctta taccgaaaat catcttctat taattccaat aacaatagat
      181 atgctccact ggttaactca cgcaccagac cgctatgctt aatccataaa ggtttaactc
      241 caaaccttac acccttttcc tctctatcat cagacatagt agcaaatata tcaaagagta
      301 attgttctgc ctttggctcc tcctgatttt tttctaccag cttattactc cctggtattt
      361 tttgtgattg ctttgaatac ttatttttag tatctattcc tgatgtatcg ttaacagaat
      421 aataatcttc aaaaaacacc tgacctgtaa ttggttctcc tgtgccattt gaattattac
      481 catattcgtc agaagaggct gatagaagat cttccaactc agctttatca ttaagaaaag
      541 gaatagcttc tctggctatc ttaccctgca attccagagc tagaagttga tttacacgta
      601 attctacatc tgattgagtc atagtttttt ggggtggagt acactttaaa ttaacctcac
      661 tttttttgtc ctggaactgg ctggttccga catcgatgtc ggtctgctcg ccaataatct
      721 cattacaaac taagatttga tgcggtattg atgttagttt acatatgtct ctaaagtact
      781 tttcatgtaa ctgtcccccc ggggggacac tttttaaaat ttgttttact tcgttagaaa
      841 gttcagttat atttaacttt ctggcaacat cacttttact tacatttttt gctcttgcta
      901 tctgtcttat tgaccatccc ttaatatctt tcaagtattt atagtgttct gctacttcta
      961 aaaaattaat tggtttatga tgttcatcta attcattatc tattgtatcc aattcacact
     1021 cttcttcatc agttaagtta taatacaact gagagggaat ttcatttaat tcaattctcc
     1081 ggcatgcttc atacctgtgc cggcctgcta caagtctgta ttgaattata ttcccctgtt
     1141 tggttccgtt atctgtaaca gcattaaccc atacagccga tttgggaata tatccccgtt
     1201 tctcgataga agccatcaga gttttaacat gaagatgatc cagaaattga catctggtgt
     1261 taaactcgga gatattaatt ttatttatat caagagaagt aaattctcct aatttttctt
     1321 tgaaaacatt ttttattgaa ccttcagaat ttatcgtgtt attgtcattt accgtatcta
     1381 tactattcat aagaaactcc ctccaaaata caaattttcg ccttttgaag ggtttttttg
     1441 tctcttactt agatttggta ttgatttttt ctgtgtaaca gcttataatt taagtgctaa
     1501 gttaagatta taagctgtta cacagttctt caataccgaa caaggtagaa acagg
//
LOCUS       DAOQWT010000426         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_100947_length_1545_cov_3.008725, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000426 DAOQWT010000000
VERSION     DAOQWT010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100947_length_1545_cov_3.008725"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1230)
                     /locus_tag="PKJ95_07530"
     CDS             complement(22..1230)
                     /locus_tag="PKJ95_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNR66124.1"
                     /translation="MATFSYRARDNSGQLFTGVLEGDSREAIIERLREMKYFIISIEQ
                     KKKSLQSTEIRLFKSIKIRDLAIFYRQFATMVSAGLTLVSCLEILSKQTENKLLAEKI
                     EDIKKSVERGSTLADAFASHPETFSKLYINMIKAGEIGGVLDDILNRMATLMEKEYEL
                     RQKIKSAMTYPMFIFGAAFVMGVFVLIFILPQFVGVFQQFGGELPYLTKMLVWFTVVF
                     NRYWYIFFAFLALIVIAFIAYQRTKQGHKNIDRLKLKIPIFGNLFLKTSINRFTRTLG
                     TLIRSGVPIIQSLRVSAESIGNDILAEAVMNAADRIKEGQSISTPLEESRVFPPMVTQ
                     MILVGEESGELETMLLNVSQFYDQEVERAVEQLTSVIEPILMVVVAGVVGVMVIAMYL
                     PIFSMVNLVQ"
BASE COUNT          499 a          304 c          285 g          457 t
ORIGIN      
        1 caaaaaaata aactaatttt actactggac taagttaacc atactgaaga tgggcagata
       61 catagcgata accattacac ctactacacc ggcaacaaca accatcagaa tgggttcaat
      121 aactgaggtg agttgctcta ctgccctttc cacttcctgg tcatagaatt gagagacatt
      181 taataacatt gtttccagct ccccactctc ttcacccacc agtatcatct gagttaccat
      241 aggagggaag actctacttt cctctaaagg agtggagata ctctgccctt ctttaatgcg
      301 gtctgctgca ttcataaccg cctctgccag aatatcatta ccgatggact cagcagatac
      361 tctgagtgac tggatgatgg gaacaccgct gcgaattaag gtacccagag ttctggtgaa
      421 acggtttatg gaggtcttca ggaacagatt gccgaaaata ggaattttta attttaatcg
      481 gtcaatattc ttatgcccct gtttggtacg ctgataagct ataaaggcaa taacaataag
      541 ggctaaaaaa gcaaaaaaga tataccagta acggttaaaa actacggtaa accagaccag
      601 catcttggtt aaatagggaa gctctccgcc aaactgctgg aatacaccta caaattgggg
      661 caggataaag atgagtacaa aaacacccat tacaaaagct gcaccaaaaa taaacatagg
      721 ataggtcata gcagacttta ttttctgacg cagctcatat tctttttcca taagggtagc
      781 catacgattt aagatatcat cgagcacacc gccgatttcc cctgctttta tcatattgat
      841 atatagtttg gagaatgttt caggatgact ggcaaaggca tcagcaaggg tcgaacctct
      901 ttctacactc tttttaatgt cttcaatttt ttcggctaaa agtttgttct cggtttgttt
      961 tgacaatatt tccagacagc tgaccagagt caaaccggca ctaaccatag tggcaaattg
     1021 cctgtaaaag atggctaaat ctctgatttt aatggattta aaaagtctta tttcagtgga
     1081 ttgaagagat tttttctttt gttctatgga aataataaaa tatttcatct cccggagacg
     1141 ttctataatc gcctcccggc tgtccccttc taatactccg gtaaaaagct gtcctgaatt
     1201 gtcccgtgct ctataggaaa aggttgccat agaatatttc accccctaat tatagtgatg
     1261 ggtaatcagt agatagtgat taattataag attataagat attgtaagat tatgacaaac
     1321 ataatcgtac atataattat atatatgtat atagtattag tataccatta aaagtcatta
     1381 attaaaatca ttgcgggtga gaatggatga gattgcttcg tcacttcgtt cctcgcaatg
     1441 acaaaaaagg aaagctcctc caatgacatt ctaattgatg gtcattggta aatagtaata
     1501 gtggcatatg ccactaggtc tcgtctaatt atatataaaa tttaa
//
LOCUS       DAOQWT010000427         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_103352_length_1523_cov_3.021798, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000427 DAOQWT010000000
VERSION     DAOQWT010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103352_length_1523_cov_3.021798"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PKJ95_07535"
     CDS             complement(<1..478)
                     /locus_tag="PKJ95_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNR66125.1"
                     /translation="MKKYVTILGSTGSIGTQTLQVIQNELENFEVLALSAWKNLELLK
                     EQILEFKPKYAVVRDDKSAKELINSLNSRTNCHILYGEYGLVKISTLSDLDIVVVAIT
                     GMAALKPALEAINAKKLICLANKEIIVSAGEIIMKKAKENNIKIIPIDSEHSGFFQC"
     gene            complement(511..1326)
                     /locus_tag="PKJ95_07540"
     CDS             complement(511..1326)
                     /locus_tag="PKJ95_07540"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNR66126.1"
                     /translation="MGEKFDKISIRTLTTVIILPIILLIIYLGGKLYYGTIILLAILV
                     FIELWHIVKHHDYQPSLMLGFILTAFFLFQEKILELCPSYDVRAIFTLIFLLIISEHF
                     LLKPKKSSIINIALTIFMAIYIGHFLSYFIEFLNLANGKVLLIFSLFCTWISDIVAYL
                     IGVKFGKNHPYPYLSPNKTIEGTIAGIFGGGICALTFYSKLPLNIYIIFVLGIVSAIS
                     GQIGDLFESLIKRNFGVKDSGTMIPGHGGVLDVIDSILFSIPTLYYIFKILIK"
     gene            complement(1310..>1523)
                     /locus_tag="PKJ95_07545"
     CDS             complement(1310..>1523)
                     /locus_tag="PKJ95_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HNR66127.1"
                     /translation="SKYLFTSGLPDPDLLIRTGGELRISNFLLWQIAYTELWFTKTFW
                     PDFSRKQLWKALSDYEKRVRKFGGKV"
BASE COUNT          530 a          241 c          222 g          530 t
ORIGIN      
        1 tacattggaa aaaaccacta tgttcactgt cgataggaat tatttttata ttattttctt
       61 ttgctttttt cattattatt tcaccggcag atacaatgat ttctttatta gccaggcaaa
      121 taagtttttt cgcgttaata gcctctagag caggttttaa agctgccata cctgttattg
      181 ctacaacaac tatgtcaagg tctgacaggg tggatatttt taccagtcca tattcaccat
      241 ataatatgtg gcagtttgtt ctgctattta aactatttat tagttcctta gctgatttat
      301 catctctgac tacagcatat ttaggtttaa actctaaaat ttgttctttt aataattcta
      361 aatttttcca ggcagataaa gctaaaactt caaaattttc aagttcattt tgaattacct
      421 gtaaagtctg cgttcctatt gaaccagtag aacccaggat tgttacatat tttttcataa
      481 taaaaagttt ttctccagta ttttaaaaga ttattttatt aaaattttaa agatgtaata
      541 taaagttggg atggaaaata aaatgctatc tataacgtct aaaactcctc catgtcctgg
      601 tatcattgta ccagaatctt ttactccaaa atttctcttt attaaggact caaatagatc
      661 accaatttga ccggaaatag ccgagacgat tcctagaaca aatattatat atatatttaa
      721 aggaagttta gagtaaaaag tcaaggcaca tattcccccg ccaaagatac cggcaatggt
      781 tccttctatg gttttattag gacttagata agggtaagga tgatttttcc caaactttac
      841 accaatcagg taagcaacaa tatcgcttat ccaggtacag aataaagaaa agatgagtaa
      901 taccttacca ttagcaagat ttaaaaattc tataaaatat gataaaaaat gtcctatata
      961 tatagccata aagatagtca gggcaatatt tataatactt gatttttttg gctttaataa
     1021 aaaatgttct gaaattatta aaagaaatat aagggtaaaa attgctctga catcataaga
     1081 tgggcataat tctaatattt tttcttgaaa caaaaagaat gctgtaagaa taaaacctaa
     1141 cattaaagag ggttgatagt catgatgttt tactatatgc catagttcta tgaaaactaa
     1201 aatagcaagg agaataatag ttccataata caattttcca cccagataaa ttattaataa
     1261 tatgataggt aatattataa cagtagtaag tgttcgaatg cttattttgt caaacttttc
     1321 ccccaaattt tctcaccctt ttttcataat cactgagagc tttccataat tgttttcttg
     1381 aaaagtctgg ccagaaagtc tttgtaaacc agagttcagt ataggctatt tgccataaca
     1441 ggaaattact aattcttaat tcaccacctg ttcttatcaa cagatcagga tcgggtaacc
     1501 ctgaagtgaa gaggtatttg cta
//
LOCUS       DAOQWT010000428         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_103458_length_1522_cov_3.879346, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000428 DAOQWT010000000
VERSION     DAOQWT010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103458_length_1522_cov_3.879346"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PKJ95_07550"
     CDS             complement(<1..710)
                     /locus_tag="PKJ95_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66128.1"
                     /translation="MKKLLQFSSLPRKILISFLVIFLIALLTAFIVVYYYGSEDIKRR
                     EIGMLETVRDLKVDNLRNWLEERKGDIQIISFDNHIREMESLFLEQNTISDEISNIKE
                     NARQYLLRYLQYFPDYQQLLIINPQTGRIILSTIKEREGEIVSDHNSFQQSARSTNIL
                     IQEIHYSEYLRNDTLDCSIPIFCLEHQGEHKIGILVARINPHISLYPLLSDIIGLGRT
                     GETVVVNREGIVQNPLRW"
     gene            complement(790..>1522)
                     /locus_tag="PKJ95_07555"
     CDS             complement(790..>1522)
                     /locus_tag="PKJ95_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNR66129.1"
                     /translation="RMKDKNIQEIEAYYNYLLENREELEQLVQGLIVPVSRFFRNPVV
                     FETIFSYILPFLLEKKKNNRERYFRFWSAGCSTGEEPYSLAILLKELFKKETEGFLIN
                     IFATDIDRKALQFAQEGIFGHESIKNTRYEWVEHYFKRQDDFFAIHSEIKEAVYFSYH
                     DLRNEKNIVPPESIFGYFDLVFCRNVLIYYDIDTQMQILKNLLKSMAPGGYLVLGESE
                     SIPARHQIKAKRILRYSNVYRKLVN"
BASE COUNT          486 a          298 c          237 g          501 t
ORIGIN      
        1 tgccatcgca atgggttttg cactattcct tctctattca cgacaaccgt ttcaccagtc
       61 ctccccagtc cgataatatc ggacagaaga ggatagagag aaatatgggg attgattctg
      121 gctactaaaa ttcctatttt atgttcgccc tgatgctcta aacagaagat tgggatagag
      181 caatctaatg tatcatttct taggtattcc gaataatgta tctcttgaat taagatgttt
      241 gtagatcgag ctgattgttg aaaagagtta tggtctgata caatctcacc ttcccgctcc
      301 tttatggtag ataaaataat ccttcccgtt tggggattga taattaataa ttgctggtaa
      361 tcggggaaat attgtaaata acgtaacaaa tattgtcggg cattttcttt gatattggaa
      421 atttcatcag aaatagtatt ttgctctaaa aacaaggatt ccatttcccg tatgtgatta
      481 tcaaaggaaa ttatctgaat atctcccttt ctctcctcca gccagttccg caaattgtca
      541 acttttaaat ctctaactgt ctccagcatt cctatctcac gtcttttaat atcttcagac
      601 ccgtaatagt aaactacaat aaaggcagtt aagagagcta tcaggaaaat taccagaaag
      661 gatatcaata ttttacgcgg taaagaagaa aattgtagca attttttcat ttcatttcac
      721 cttctcccta tgataaagat taaggccaat cattagacct aatgaatttg gtccgaaagt
      781 tagaaataat taattgacta attttctata gacattacta tagcggagta tcctttttgc
      841 ttttatctga tgccgggcag gaatagattc agattcacct aaaaccagat atccgccagg
      901 agccatactt ttcaataaat tttttaatat ttgcatctgt gtatctatat cataatagat
      961 taagacattc cggcaaaaaa caaggtcgaa atatccaaag atactctcgg ggggaacaat
     1021 gtttttttca ttccttaaat catgatagga aaaataaact gcctctttaa tctcactgtg
     1081 aattgcaaaa aagtcatcct gccttttaaa ataatgctct acccattcgt agcgggtatt
     1141 tttaatgctc tcatgaccaa aaatcccttc ctgagcaaat tgcaaagcct tcctatcaat
     1201 atctgtggca aaaatattaa ttagaaaacc ttcagtctcc tttttaaaca attcttttaa
     1261 taaaatagcc agagaataag gctcctctcc tgtagaacag cccgcagacc aaaaacggaa
     1321 atatctctct ctattattct ttttcttttc gagcaagaaa ggcaggatgt agctgaaaat
     1381 tgtttcaaag acaactggat ttctaaaaaa acggcttacc ggaacgatta atccttgaac
     1441 taattgctcc agctcttccc tgttttctaa taaataattg taatatgcct caatctcctg
     1501 aatattttta tccttcattc tt
//
LOCUS       DAOQWT010000429         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_103672_length_1520_cov_3.879863, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000429 DAOQWT010000000
VERSION     DAOQWT010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103672_length_1520_cov_3.879863"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..>1520
                     /locus_tag="PKJ95_07560"
     CDS             98..>1520
                     /locus_tag="PKJ95_07560"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNR66130.1"
                     /translation="MKEETLTNEQLKALIGQEVNRQHKKHRPMTVPTLEEGCYKTLKA
                     DDILYAFSDKEKVTIVTKDGKKHETTLTIKNIADLLQKEPSFKITHKNYIVNMSEVTR
                     SHKHGSGYGLSFRDIPDIVPVTEAHKKEIAEYLNINDLAVLDGYKKLMTILSRNKIRD
                     FDEDITTFDRERLYKEFSKSSGEFYLTNLMYNLIYQAFTWIKEGKMEPIEGNIRSFWY
                     SHVKVALSTLDMVKEKNYDLMIQCFNKFITEWRITTYKSFGFWDANYYASKIGVKNPH
                     IILMAEKNGHLFILEKLHREYDITTIALGGQGSSLSIEYFVDAMEEAEIDKKQTFYIF
                     SSVDYDPSGYDIKKSFIDSLKYEGIKDIKVFDIVTLDRFTPEEIQSKKYPLFDADEIP
                     PKDWETRVKKWVKKTKGIGPKGSPWKDRAYGIESDAMVRKIRDVFLELAKPYLKKDPS
                     ITTGEPGKKETLSMTEKKIMRFCK"
BASE COUNT          621 a          289 c          282 g          328 t
ORIGIN      
        1 aaaacaacca gacggaagta aaagaatatc tgggaataaa actgccaaac gccactgcag
       61 aggagccgcc gccggaagga ggagaagagc cgccgcaatg aaagaagaaa cattaacaaa
      121 cgaacagcta aaagcactta taggacagga agtaaacaga cagcacaaga aacacagacc
      181 catgactgtt ccgactcttg aagaaggctg ctataaaact ctaaaagcag atgatatcct
      241 ttatgccttc tcagacaaag aaaaggttac tattgtcaca aaagacggaa aaaaacatga
      301 aacaactcta accataaaaa acatagcgga cctgctgcaa aaagaaccat cctttaaaat
      361 cactcataaa aactatattg ttaatatgtc cgaagtaacc agaagccata agcatggcag
      421 cggctacgga ttatctttca gagatattcc cgacatagta cctgtaacag aagcccataa
      481 aaaagaaata gcagaatatc tgaacatcaa tgaccttgca gttttagacg gatacaaaaa
      541 gttaatgaca atactttcaa gaaacaaaat aagagacttc gatgaagata taacaacctt
      601 cgacagagaa aggttatata aagaattttc aaaatcttca ggagaatttt acctgacaaa
      661 cctcatgtat aatctcatct atcaggcttt tacctggata aaagaaggaa aaatggaacc
      721 tatagaagga aacataagat ccttctggta ctcacatgta aaagttgcac tctcaaccct
      781 ggatatggta aaagaaaaaa actatgacct catgattcag tgtttcaaca aattcattac
      841 agaatggaga ataacaactt ataaatcatt cggtttctgg gatgccaatt attacgcaag
      901 caaaataggc gtaaagaatc cccatataat cctcatggca gaaaagaatg gacacctgtt
      961 catactggaa aaacttcaca gagaatatga tattactaca atagccctgg gaggacaggg
     1021 gtcatcactg agtatagaat acttcgtaga tgctatggaa gaagctgaaa tagacaaaaa
     1081 acagactttc tatatctttt catcggtaga ctatgatcca tcgggatatg atataaaaaa
     1141 atcctttata gactctctta aatacgaagg cataaaagac attaaagtat tcgacatagt
     1201 taccctggac aggttcaccc ctgaagaaat acagagcaaa aaataccctc tctttgatgc
     1261 agacgaaata cctcctaaag actgggagac aagagtaaag aaatgggtaa agaaaaccaa
     1321 aggtataggg ccgaaaggat ctccctggaa agacagagcc tatggaatag aatcagacgc
     1381 aatggtcagg aaaataagag atgtcttttt agaactggca aagccttatt taaaaaaaga
     1441 cccatctata acaaccggcg aaccgggcaa aaaagaaaca ctctctatga cagaaaaaaa
     1501 gattatgaga ttctgcaaag
//
LOCUS       DAOQWT010000430         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_103676_length_1520_cov_3.477133, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000430 DAOQWT010000000
VERSION     DAOQWT010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103676_length_1520_cov_3.477133"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKJ95_07565"
     CDS             complement(<1..285)
                     /locus_tag="PKJ95_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006632324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNR66131.1"
                     /translation="MKAIILCAGKGTRLRPLTYTSAKHLIPLANKPALEYGIEKITEC
                     GIEDIGIIIGEETGEDIKKAIGDGKRWGVRISYILQSKPLGLAHAVKIART"
     gene            complement(310..>1520)
                     /locus_tag="PKJ95_07570"
     CDS             complement(310..>1520)
                     /locus_tag="PKJ95_07570"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNR66132.1"
                     /translation="GKSFCVHEVNHREKLTLQHDCPNHREAIKLIIDTLVHPKYGAIN
                     NLSDISAVGHRVVHGGEKFSKSVLINEEVLKTFKELSILAPLHNPPNIMGIEAAKGLL
                     PDIPHAAIMDTAWHQTMLPYAYMYAVPYEWYEKYRIRRYGFHGTSLLYVAKRAAVLLN
                     KDPFDCNLISCHIGNGVSINAVKSGISYDTSMGFTPLEGAIMGTRAGDHDAALDFYIM
                     QKEGYTPKEIDAILNKKSGILGITGKYTDRRDVVKAAGEGEERAKLAIDMEVYRLKKY
                     IGAYTAVLGRLDALIFTAGVGEMSTTIRGKILDGLELIGIKYDPAKNSLAKTRNKESD
                     ISAKDSPVKVFVIPTDEELVFVEDVVALIEGNYDIHTNFKYTFQEKNYKNLMREKAYK
                     KEQKELFEKK"
BASE COUNT          449 a          317 c          225 g          529 t
ORIGIN      
        1 agttcgggca attttaacag catgcgccag accaagtggt tttgattgta aaatatagct
       61 tattcttact ccccatctct ttccatctcc aattgccttt ttaatatctt caccagtttc
      121 ctctccgatt attataccaa tgtcttcaat cccacattca gtaatcttct caatgccata
      181 ttctaatgcg ggtttattag ctaaaggaat taaatgttta gcactggtgt aggttaaagg
      241 ccttaaacgg gtccctttac ctgcacataa tataattgct ttcataaaat attttctcca
      301 atataaaaat catttctttt caaataattc tttttgctct tttttataag ctttttctct
      361 catcaaattt ttataatttt tttcttgaaa agtgtattta aaattagtat gaatatcata
      421 attgccttca attaaagcaa caacatcctc aacaaagact aattcctcat cggtaggaat
      481 aacaaatact ttgacagggg aatcctttgc cgaaatatcg gattctttat ttctggtttt
      541 agcgaggcta ttctttgctg gatcgtattt gattccaatc agttctaaac catccaatat
      601 tttaccacgt attgtggtgc tcatttcccc aactccggca gtaaaaataa gggcatccaa
      661 tctacccaaa acagcagtat aggcaccaat atattttttt aatctataaa cttccatatc
      721 tatggcaagt ttcgctcttt cttctccttc gccagcagct ttgactacat ctcttcggtc
      781 agtatattta cccgtaattc ccaatattcc gcttttttta tttaatatgg catcaatttc
      841 tttcggtgta taaccctctt tttgcataat atagaaatct aaagcagcat catggtctcc
      901 ggctctggtg cccataatag caccttctaa tggggtaaaa cccatactgg tatcataaga
      961 gataccactc ttgaccgcat ttatacttac tccattccca atatgacagc taatgagatt
     1021 acaatcaaat gggtctttat ttaaaagtac tgctgccctt ttggcaacat aaagcaaaga
     1081 agttccatga aagccatatc ttctgattct atacttctca taccattcat aaggaacagc
     1141 atacatgtag gcatatggta gcatagtctg atgccaggcg gtatccataa tagcggcgtg
     1201 aggaatatct ggtagtaatc ctttagctgc ctcaatgccc attatattag gcggattatg
     1261 caaaggagct agaattgata actctttgaa agttttcaaa acttcctcgt taattagaac
     1321 agacttggaa aacttttcac ctccatgtac tactctgtga ccaacagctg agatatcaga
     1381 taaattattt atagcaccat atttcggatg aacaagagta tcaattatta attttatagc
     1441 ttctctatgg ttagggcaat catgttgtaa ggttaacttt tccctatgat taacttcgtg
     1501 gacacagaag gattttccta
//
LOCUS       DAOQWT010000431         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_103682_length_1520_cov_3.309215, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000431 DAOQWT010000000
VERSION     DAOQWT010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103682_length_1520_cov_3.309215"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKJ95_07575"
     CDS             <1..291
                     /locus_tag="PKJ95_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNR66133.1"
                     /translation="SALSMSVAVSIGYKEGWDSALFALASVFTIVVMADAAGVRRATG
                     EQAKVLNKLMLIFFKENRVKSERMQELIGHTPFEVIVGAIIGLAIATTFSLY"
     gene            398..1285
                     /locus_tag="PKJ95_07580"
     CDS             398..1285
                     /locus_tag="PKJ95_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNR66134.1"
                     /translation="MDNILSEKEVAQLKKAQQDELVGEQVYGILAKLTKDTHNSKILA
                     RISNEEKKHAEIFKQYTKTDLPVDRFKVFFYVFISRVFGLTFGIKLQEKGEQEAQKNY
                     KEMLKVIPEMKEIIEDEEKHEIELINMIYEEKLSYMSSVVLGLNDALVELTGALAGFT
                     LSVQNSRIIAFLGLITGISASLSMAASEYLSTKAEQDPEAQKRAGKSALYTGIAYILT
                     VIALIIPYFLSSNYILSLIATVIVALTIIFVFNYYISVANDYDFKRRFAEMALISMGV
                     AALSFLIGHLVNTFLGFEL"
BASE COUNT          523 a          211 c          284 g          502 t
ORIGIN      
        1 tctgcccttt caatgagtgt tgcagtttct attggttata aagagggctg ggattctgca
       61 ctgtttgctt tagcgagtgt atttactatt gtagtgatgg ctgatgcggc tggggtaagg
      121 agggcaaccg gggaacaggc aaaggtgcta aataaactta tgctgatatt ttttaaggaa
      181 aatagagtga agagcgagcg tatgcaagaa ttaataggac atacaccctt tgaggtaatt
      241 gtgggagcta ttattggttt ggcaatagcg accacgtttt ccctttatta gttttattaa
      301 tttggtttat aattttttat ttaaccttta atatttagat ttagattatt ccagaaatat
      361 taatcaataa atttatttaa agataggaaa tgtatttatg gacaatattt tgagtgaaaa
      421 agaggttgct cagctaaaaa aagcacagca agatgagctt gttggagaac aagtttatgg
      481 aatactggct aaattaacta aagataccca taactccaaa atattagcca gaataagcaa
      541 tgaagagaaa aaacacgctg aaatattcaa gcaatatacc aaaacagact taccggtaga
      601 caggtttaaa gtattttttt atgtttttat atcccgggta tttggcttga catttggaat
      661 taaattacag gaaaaaggag aacaggaagc tcagaaaaat tataaagaga tgcttaaagt
      721 tataccggag atgaaagaaa taattgaaga tgaagaaaaa catgaaatag aattgattaa
      781 tatgatttat gaggaaaaac tttcctatat gagttctgta gtattaggtt taaatgatgc
      841 cttagttgaa cttaccggtg ccctggcagg ttttaccctc tctgtgcaga attcaagaat
      901 aatagcattt ttaggattaa ttactggtat ctctgcttct ctttctatgg cggcaagtga
      961 atatctgtcc acaaaagccg agcaagatcc agaagcacag aaaagggcag gaaaatcagc
     1021 attatatacc gggattgctt atattctgac tgttatagca ttaattatcc cttatttcct
     1081 ctccagcaat tatattctta gtctgatagc tactgttata gtagcattaa ctataatctt
     1141 tgtatttaat tattatattt ctgtggcgaa tgattatgat tttaaaagaa gatttgcaga
     1201 gatggccctt atcagtatgg gagtggcagc actttctttt ctcattggac atttagtaaa
     1261 taccttttta ggctttgaac tataactctc gcactctctt tatacagcca ggtttttata
     1321 ttagaaccag cagaataaag aatttcaaaa atttttaaaa atattatact cactattgac
     1381 agatcataaa gttccagcta acatatgaac aatttaatct tttagaaata aaaaatatgt
     1441 taaagaataa aacattagcg taacagaaaa aagaaatatt ggtaatctat tttatttctt
     1501 ctactttatt cctgaggtga
//
LOCUS       DAOQWT010000432         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_103855_length_1519_cov_2.342896, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000432 DAOQWT010000000
VERSION     DAOQWT010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103855_length_1519_cov_2.342896"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKJ95_07585"
     CDS             <1..229
                     /locus_tag="PKJ95_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNR66135.1"
                     /translation="VARVAKKYNIPVVSISALIDNSGWIVKEHGIDYLIKPENPPLSM
                     EFSKFKKIEILSKTVKDFLDKYDRTISANRE"
     gene            358..600
                     /locus_tag="PKJ95_07590"
                     /pseudo
     CDS             358..600
                     /locus_tag="PKJ95_07590"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(749..>1519)
                     /locus_tag="PKJ95_07595"
     CDS             complement(749..>1519)
                     /locus_tag="PKJ95_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNR66136.1"
                     /translation="YTYQKLKKYNISIPKYFVFNLKNRFRRKQLLHEIEHIGYPLMVK
                     PAGGGDSIGITSKSVIHNIDELRYIIEYLRRTLGPEKIIIEQYLSGQEFTVGVLSGEK
                     MYILPIISFPENWGIRYTETKNKEYRMYNQFNIVNERNALFYSLIDISVKSFQAVKAN
                     DIIRIDIKKDQLGNLYVIDINGSPALTKRASIAFMASKAGLSQSQLIMLVFYNSIVRN
                     GLSPSKYLEDLIKPLQKKLKPYDHGQLMEALLVDTETE"
BASE COUNT          514 a          232 c          230 g          543 t
ORIGIN      
        1 agtagccaga gtagcaaaaa aatataacat accagtagta tcaatcagcg cattaattga
       61 taattctgga tggatagtca aagaacatgg gatagattat ctaattaaac cggaaaaccc
      121 acctctttct atggaatttt ctaagtttaa aaagatagaa atattatcaa aaacagtcaa
      181 agacttttta gataaatatg atagaactat ttcagcaaac agagaatagt atttaaaaat
      241 tgtatataat tgaggattgg ttatttagaa aaattaaaga atatagtggt ttagcccatt
      301 aagaaggagt tgatcaactc caaacttttt aatcaataaa ccgtttttgt aaaattagga
      361 tttgatcctt tgtgggctgc tatgatggtt tgcattaatt tacaaatggc ctttcaaaca
      421 ccacctatgg ctatgtcaat ttttgtctgt agaggaacag cgcctcctga attgggtgtg
      481 acaatggatg atattattaa aggggtcatt ccctttatag tattaataat attttgcttg
      541 gtactctgta ccatattccc tcaaatcatt acctggttgc cagaaaagat gattggttaa
      601 tataattgaa ttaaagaaat ctaatcaatt ataaagggat acacaattaa taaaatacgg
      661 gtttgttata gacccatatt tttacttaaa atttttagtt ttctataaaa aataatctta
      721 tctatttttt aaggtactac ttattctctt actcagtttc agtatctact aaaagtgctt
      781 ccattaattg gccatggtcg taaggtttta attttttttg cagaggttta atcagatctt
      841 ccagatactt gctgggggat agaccatttc ttactatgct attgtaaaaa actaacataa
      901 ttaattgact ttgtgacaac cctgctttac ttgccataaa agctattgat gcacgtttgg
      961 taagagcagg ggagccattg atatcaataa catataagtt gccaagttgg tcctttttaa
     1021 tatctatacg aatgatgtca ttagctttga cagcctgaaa actttttacc gatatatcaa
     1081 tcaatgaata aaataaggca ttcctctcgt taacaatatt aaactgattg tacattctat
     1141 attctttatt ttttgtttca gtataacgaa ttccccaatt ttcaggaaaa gagataatag
     1201 gtaagatata cattttttca cccgataaaa ccccaacggt aaattcctgt ccggataggt
     1261 attgttcaat tattattttt tctggcccta aggttcttct taaatattct attatgtacc
     1321 tcaattcatc aatattatgt ataactgatt tcgaagttat acctatacta tctcctccgc
     1381 cagcaggttt aaccattaac ggatatccaa tgtgctctat ttcatgtaat aattgtttcc
     1441 ttcgaaaacg attcttgaga ttaaacacaa aatatttggg aatagaaata ttatatttct
     1501 ttaatttttg ataagtata
//
LOCUS       DAOQWT010000433         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_103918_length_1518_cov_3.168148, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000433 DAOQWT010000000
VERSION     DAOQWT010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103918_length_1518_cov_3.168148"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1253)
                     /locus_tag="PKJ95_07600"
     CDS             complement(210..1253)
                     /locus_tag="PKJ95_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNR66137.1"
                     /translation="MALPRLTAQQIKEDPEQQLRTFKRSKDFLIAIDTDGCITDNMNG
                     KQILIFHPQFMEFYQLWGIESYFREVAEYYNLFSVDRGCNRFIAIQLTLKALKKRKDV
                     QEVLKQKGIALPDSKPLDDYIQFAKENKRGLGNPSLEEYVNKYSTNFALYKLLGWSEA
                     VNRTFPHISAKIPPFEGVKDNLTLMHEVADILVVSKTPYDDLANYWEAQGIEQYVQMI
                     AGQEMGSKGHHIAMAKKAGHYRDEQILMLGDGNGDLEAVKENNGLFYPVSPGYEQESW
                     DNFPEALKQFVKEDYRGRFEDRLLDEFEQVLLTSPPWEKADYNHISAYREKQEIRKAL
                     YQRFNPEGRLLIL"
BASE COUNT          427 a          338 c          227 g          526 t
ORIGIN      
        1 gcaaccctgt tacctaaagt ggagtagaca gatgaagaat agagaacagc accacccacg
       61 tttttttcta catttccaga atagatattt tcgtctaaag aaatgtgtcc gataacaaaa
      121 aaatcataca tatttcacct ttattaatgt attccttttt ctacgctttt cctgtttctt
      181 ctcgtattaa ccagtaaaag atagttctgc tacaaaatca gcagtcttcc ttccggatta
      241 aacctctgat aaagagcttt tctgatttcc tgtttttctc tataggcaga aatatgatta
      301 taatcggctt tctcccatgg tggtgaagtt aacagtacct gttcaaattc atctaaaagt
      361 ctgtcctcaa accttcccct ataatcttcc ttaacaaatt gttttaaagc ttccggaaaa
      421 ttatcccagg attcctgctc atatccggga gaaacaggat aaaaaaggcc attattctcc
      481 tttaccgcct ccaaatctcc attcccatcc cctaacatta ggatctgctc gtcgcggtaa
      541 tggccagcct tttttgccat tgcaatatga tgtcctttgg accccatttc ctgtccggca
      601 atcatctgca catattgttc aataccctgt gcctcccagt aattagccag atcgtcatag
      661 ggtgtttttg ataccactaa aatatcagca acctcgtgca taagggtaag attgtctttt
      721 actccctcaa agggtggaat tttagctgaa atgtgtggaa aagtacgatt taccgcctca
      781 ctccagccta acaacttgta cagggcaaaa ttagtggaat atttgttgac atattcttcc
      841 agactggggt tacctaaacc tctcttattc tctttggcaa attgtatata atcatccaat
      901 ggtttactat caggtaaggc aatacctttt tgttttaaaa cttcctgtac atcttttctc
      961 ttctttaaag ctttcagggt tagttgtata gctataaaac ggttacatcc gcgatctaca
     1021 gaaaaaaggt tataatattc tgcaacttct ctaaagtatg attcaatacc ccataactgg
     1081 tagaactcca taaactgggg atggaagatg agaatttgtt ttccattcat attatcagta
     1141 atacaaccat cggtatcgat ggcaattaaa aagtctttac tccttttaaa ggttcttaac
     1201 tgttgttccg ggtcttcctt aatctgttga gcagttaacc ttggtagtgc catttttctt
     1261 ctcctctttg tataattatt agtagataat taatatcctt atcccgtccc gtctgttgaa
     1321 cccaccaatc actcatcact ctttacttat cactgttcac tcattactat ttatcgttta
     1381 cttatcactg ttcaccaatc attatttatt attcactcat cacttttcac ttgtcactta
     1441 ttaaaaccct gccggtgtat attttccaca tttcgatggt agaaaaatat ctttaaatct
     1501 ggcaaactca ggtaatgg
//
LOCUS       DAOQWT010000434         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_104085_length_1517_cov_2.114227, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000434 DAOQWT010000000
VERSION     DAOQWT010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104085_length_1517_cov_2.114227"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..>1517
                     /gene="ligA"
                     /locus_tag="PKJ95_07605"
     CDS             277..>1517
                     /gene="ligA"
                     /locus_tag="PKJ95_07605"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNR66138.1"
                     /translation="MAEKKNKLEIKKEIEGLREEINRHNYYYYVLDQPIVSDYEYDQL
                     MRRLNKLEQENPEFIASHSPTQRVGAKPLEQFITARHLTPMLSLSKAFSEKELFDFDT
                     RIKKNFSRYYFDYVVELKIDGLAIALVYENGILNRGATRGDGVIGEDVTQNLRTIGSI
                     PLELREFKGIDTIEAYGEVYMNRESFKNLNEQRSKKLENLFANPRNAAAGSVRQLDPA
                     VTASRQLDTFIYQATFPGNDLFSTHMDMLVFLKNAGFRVNSNIKLCATIEEAVEYCYY
                     WQNRKKELNYDIDGMVIKVNQLTLRERLGSTSRSPRWAIAFKFPAEQMTTIVKDIIVG
                     VGRTGALTPVAVLEPIFISGSKVQRATLHNEDEIKRKDIRIGDTVLVQKAGEIIPEIV
                     QVIKEKRSGQEKIFQMPDRCP"
BASE COUNT          519 a          235 c          321 g          442 t
ORIGIN      
        1 aactgcaaaa gtcaggttat aatgaacagt ccatctctgg ttcaatctgt taaaattaac
       61 ttatgtaaaa gattgtgcca taaaaatgta tttcccctat gttcccctta aaggacagaa
      121 atatttttta gaaattatct tctgattatt attatcggag agaatatctg tcacaattta
      181 tataaaaaat ccttaacaat gaatatagtt tttactatac tattgagctt tttgttcttt
      241 ttgatatata ctaaagaaaa aaaagaataa actaatatgg cagaaaagaa aaataagctg
      301 gaaataaaaa aagaaattga aggactgaga gaggaaatca atagacataa ctactactat
      361 tatgtccttg accagccaat tgtctctgat tatgagtatg accagctgat gagacgacta
      421 aataaattag agcaggagaa tccggagttt attgcttccc actctcctac ccagagggtg
      481 ggggcgaaac ctttggaaca atttattact gccagacacc tgactcctat gttaagcctt
      541 tcaaaagctt tttccgaaaa agaattattc gatttcgaca cacgtattaa aaagaatttt
      601 tcccggtact attttgatta tgtggtggaa ttaaagattg atgggctggc aattgcctta
      661 gtttatgaaa atggtatttt aaatcgtggt gctaccagag gtgatggtgt gatcggtgaa
      721 gatgttaccc agaatcttag aaccatagga agtatacctc ttgaattgag agagtttaaa
      781 gggatagata caattgaggc atacggagaa gtgtatatga acagggaaag ttttaaaaat
      841 cttaatgaac aacggtctaa aaaattagaa aacctgtttg ccaatcccag aaatgccgcc
      901 gccggttctg tacggcagct tgatccggca gtaacagcca gcaggcaatt ggatactttt
      961 atttatcagg ctacttttcc gggcaatgat ttattttcta cccatatgga tatgcttgtt
     1021 tttctcaaaa atgccggatt cagggttaat agcaatataa agctttgtgc tactattgag
     1081 gaggcggttg aatattgtta ttactggcag aatagaaaaa aagagttaaa ctatgatatt
     1141 gatggaatgg taattaaggt taatcagtta acattgagag aaagattagg gtcaaccagc
     1201 aggagcccca ggtgggcgat agcatttaaa tttccggctg aacagatgac taccatagtg
     1261 aaagatatta ttgtgggagt tgggcggaca ggagcactta ctcctgtggc agtattagaa
     1321 ccaattttta tctctggttc caaagtgcaa agggctacgc tgcacaatga agatgaaata
     1381 aagaggaagg atataagaat tggtgatacc gtactggtcc agaaagctgg tgaaataatt
     1441 cctgagattg ttcaggtaat taaagaaaaa agaagtgggc aagaaaagat tttccagatg
     1501 cctgaccgct gtcccat
//
LOCUS       DAOQWT010000435         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_104612_length_1512_cov_3.501716, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000435 DAOQWT010000000
VERSION     DAOQWT010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104612_length_1512_cov_3.501716"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1512)
                     /locus_tag="PKJ95_07610"
     CDS             complement(<1..>1512)
                     /locus_tag="PKJ95_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNR66139.1"
                     /translation="VSVVNALSEWLEVTVNRDNKIYWQRFERGKPVTDLKVIGRSTKT
                     GTKITYRSDPEIFEDITINFDIINHRLKELAFLNAGIKIELIDEREKDKTVLFKYDGG
                     IIEFVKYLNKNRDVLFSKPFFISGKKDDIEVEIAIQYNTGYLENVLSFANNINTEEGG
                     THLAGFKAALTKAINDYLKNDSIPEVKKFSLKGMEQGLSGNDVREGLTAVVSVKLLNP
                     QFEGQTKTKLGNSEVRGIVSSVVNDKLCAFFIENPDTIQKIVAKSISAYKAREAARRA
                     RELVRQKNGFSLGSLPGKLADCSERDPFHRELFLVEGDSAGGSAKQGRDRKFQAILPL
                     RGKILNVEKARLHKILTNNEIRAIITASGLQIGDDDDLDLAQLRYHKIIIMTDADVDG
                     EHIKTLLLTLFYRYMRLLIEKGKVFIARPPLYKLSYGQKNEYFFNEEEMQKKIKELKV
                     SYNIQRYKGLGEMNPTQLWETTMNPETRVLKRVELEDALEADEMFTILMGGKVE"
BASE COUNT          402 a          335 c          249 g          526 t
ORIGIN      
        1 ttcaaccttt ccgcccatta ggatggtgaa catttcatct gcctctaagg catcttccag
       61 ctcaactctc tttaaaaccc tggtttcagg attcatagtg gtttcccata actgggtagg
      121 attcatctct cccaaccctt tataacgttg aatattatag cttactttta attccttaat
      181 ctttttttgc atctcttctt cattgaaaaa atattcattt ttttgtccat aactcaattt
      241 atagagggga ggtctggcaa taaacacttt tcctttttca attaacagtc tcatataacg
      301 ataaaaaagc gttaagagta gtgttttaat atgttctccg tccacatcag catcggtcat
      361 aataataatc ttgtggtacc ttaattgagc cagatcaagg tcatcgtcat ctccaatttg
      421 taaacccgaa gcagtaataa tggctctaat ttcattattt gtcaaaattt tatggaggcg
      481 ggctttctcc acatttaata ttttacctct taagggcaag atagcctgga atttgcggtc
      541 tctgccctgt tttgctgagc cgccggctga atctccctct actaaaaaaa gttcccgatg
      601 gaaggggtca cgttctgaac agtccgctaa tttacccggt agagaaccca ggctaaaacc
      661 atttttctgt ctcactaact ctctggctct tcgggcagcc tcacgggctt tataggctga
      721 gatagatttt gccactatct tttgtatggt atcggggttt tcaataaaga aggcacatag
      781 tttatcgttc actacagatg aaacaattcc cctcacttca ctattgccca actttgtttt
      841 ggtctgcccc tcgaactggg gatttaataa ttttacacta acgacagcag tcagtccttc
      901 tcggacatca ttgccggaga gtccctgctc cattcctttt aaagaaaatt tcttgacctc
      961 gggtatgctg tcatttttaa ggtagtcgtt aattgctttg gtaagagcgg ctttaaaacc
     1021 agctaagtgt gttccaccct cttcagtatt aatattgttg gcaaaagaaa gaacgttttc
     1081 caaataacca gtattatatt ggatagctat ctctacctct atatcgtcct ttttcccact
     1141 gataaaaaat ggtttactga aaagcacatc cctgttttta tttagatatt taacaaattc
     1201 gattattcca ccatcatatt taaagagcac ggtcttatct ttttctcgtt catcgattaa
     1261 ttcaattttt attccggcat tcaaaaaggc taattccttt aaccggtgat ttatgatatc
     1321 aaaattaata gtgatatctt cgaatatttc tggatccgaa cggtaagtaa tttttgttcc
     1381 tgtcttagta ctgcgtccta tcactttcaa atcggtaacc ggtttccccc tctcgaatct
     1441 ctgccagtat attttattgt cccggtttac ggtaacctct aaccattcag ataaggcgtt
     1501 taccacagat ac
//
LOCUS       DAOQWT010000436         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_105194_length_1507_cov_2.745179, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000436 DAOQWT010000000
VERSION     DAOQWT010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105194_length_1507_cov_2.745179"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /gene="panB"
                     /locus_tag="PKJ95_07615"
     CDS             complement(<1..523)
                     /gene="panB"
                     /locus_tag="PKJ95_07615"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNR66140.1"
                     /translation="MAQKKTRLDFIEMKKKGEQVAWMTAYDFPMASFIEQAGMDMILV
                     GDSLGMVVLGYQGTIPVTMEDCISHCKAVRRGAPNTFCIGDMPFMSYQISSEEAVRNA
                     GRFLKEADMDAVKLEGGIRVCKQIRAIADAGILVMGHIGLTPQSSGQLGGFKAQGRDA
                     TSARELIKDALAIQ"
     gene            complement(570..>1507)
                     /locus_tag="PKJ95_07620"
     CDS             complement(570..>1507)
                     /locus_tag="PKJ95_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNR66141.1"
                     /translation="MHIRLLLLTFFLQFFPDLVKNGHVYILETPLFRVRNKQETIYCY
                     SEEEKQEAIKKLGTKPEITRFKGLGEISPDEFARFIGSDMRKEPVIVMPEKASPVKIE
                     ATKRYGAEVVLFGKDSNDVTNKANEIAYQEGLTFLSPFDDEEVIAGHGVVGLDIIKEK
                     PDSEVIICPVGSGAFISGILLALKETNPRIKIIGVEPLNSNAMWLSLQANKIVEKERI
                     DTIADGLALKKPGELPFQIVKKYVDDIVLVNEDEIKRAVLLLLERAKLLVEPSGAVSI
                     AALMFGKIDIKNKEIVAILSGGNVELHKLADFIKN"
BASE COUNT          393 a          380 c          249 g          485 t
ORIGIN      
        1 cctggatagc aagggcatct ttaatcagct ctcgggcgct tgtcgcatct ctgccctgtg
       61 ctttaaagcc acctaattgt ccggaactct gaggggttaa gccaatatga cccattacca
      121 gtataccggc atcggcaatg gctctaatct gtttacatac tctgattccc ccttctaatt
      181 tcactgcatc catatccgcc tcttttaaga aacgtcccgc atttcttacc gcctcctcag
      241 aagatatctg ataggacata aagggcatat caccaatgca gaaggtattg ggtgcaccgc
      301 gacgcaccgc tttacaatgt gagatacagt cttccatggt taccggtatg gtaccctgat
      361 atcccaggac taccattccc agtgaatcac ccaccagaat catatccatg ccagcctgct
      421 ctataaaaga ggccatggga aaatcgtatg ctgtcatcca ggcaacttgc tctcccttct
      481 ttttcatctc tataaaatcc aagcgagtct tcttttgtgc catctttgaa acctcccttt
      541 gttttatttt tctaattatg ttttcttttc taatttttaa tgaagtctgc cagtttatgc
      601 agctctacat ttcctccact taatatagcc actatttcct tattttttat atcaattttg
      661 ccgaacataa gtgctgctat cgagactgca cctgagggtt ctaccagtaa ttttgccctt
      721 tccaggagta ggagtactgc ccttttaatc tcatcttcat tgaccagtac aatgtcatct
      781 acatattttt taactatttg aaagggcagt tcgcccggtt ttttcaaagc cagtccatca
      841 gcaatggtat caattctttc tttctcaact attttattgg cctgcagaga aagccacata
      901 gcattggaat tcaatggttc cactccaatt atttttatac gcggatttgt ctcttttaat
      961 gccaatagaa ttccagaaat gaaagcaccg cttcccaccg gacaaataat tacctcacta
     1021 tccggctttt ctttaatgat atccaatcca accaccccat gcccggcaat tacttcctca
     1081 tcatcaaaag ggcttaaaaa tgttaagcct tcctgataag caatctcatt tgctttatta
     1141 gtaacatcat tagaatcttt tccgaatagt accacctctg ctccatatct cttagtggcc
     1201 tctattttaa ctggtgaagc tttttccggc atcacaataa ccggttcttt gcgcatgtcg
     1261 gacccaataa aacgggcaaa ttcatcggga gaaatttcac ctaatccttt gaaacgggtg
     1321 atttccggtt tggttccaag tttcttaatc gcctcctgtt tctcttcttc actatagcaa
     1381 taaatagtct cctgtttatt acgtacccgg aacaagggcg tctctaaaat atatacatgt
     1441 ccgtttttta ccaggtcagg gaaaaattgt aagaagaaag tcagcaacag cagcctgata
     1501 tgcatgc
//
LOCUS       DAOQWT010000437         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_105292_length_1506_cov_3.456237, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000437 DAOQWT010000000
VERSION     DAOQWT010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105292_length_1506_cov_3.456237"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..946)
                     /locus_tag="PKJ95_07625"
     CDS             complement(29..946)
                     /locus_tag="PKJ95_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNR66142.1"
                     /translation="MHTNTRILANKFEYLDPKSLSEALNILDEYQKKNIKILAGGTDL
                     LVKMKTIELNIDYLLYIKEIAELNFINTSNGLSIGATIPFSHIIKEDKIKTEYTALYE
                     AIQAIAAPAIRNMGTVAGNIGNASPAADTVPALIVFDAQVLIESKQSKRTLFLKDLFL
                     GPGKTVLRNDELITRIEVPEVKPNTGSAFIKKSRVKADLAKINLAVAVQRKGQNCENC
                     KIAFGSVAATVVRAENTEKLLEGKALTEELISKAAQGVADEIKPIDDIRSTAEYRIAM
                     SREMLQDALSLAWARTYSEAKPNLKGGGE"
     gene            complement(936..>1506)
                     /locus_tag="PKJ95_07630"
     CDS             complement(936..>1506)
                     /locus_tag="PKJ95_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNR66143.1"
                     /translation="AEIADHARINSVGTFVYTSTLRQKNCPPCFITYFTEVEVNTETG
                     EISVPRVVMMGDSGTIINPELWKGQILGAYALGIGLGKLEDIPYNKESGKLNCNGLIT
                     DFKVPITTDMPHTDNITVEHAHTYEPTGPFGAKGIGEAALSAVASSFGNAVYDAVGIR
                     FHKLPITPEVMLKALQDKNRIGGKNKDAH"
BASE COUNT          420 a          335 c          258 g          493 t
ORIGIN      
        1 aaatttaata ggaatccttc tcaaggtatt attcacctcc acctttaaga ttgggctttg
       61 cctccgaata ggtcctagcc caggctaaac tcaaagcatc ttgaagcatt tcccgggaca
      121 tagctatacg gtactccgca gttgagcgaa tatcatctat aggttttatt tcatctgcca
      181 ctccctgagc tgctttagag attaactcct ctgttaaggc tttcccctct aacagttttt
      241 cagtattttc tgcccttacc accgtggctg ctacagaacc gaatgcaatc ttacaatttt
      301 cacagttctg cccctttctc tgaacagcaa cagctagatt tatcttagcc agatctgctt
      361 ttactctgct tttcttgata aaggcactac cggtattggg ctttacctcc ggcacttcaa
      421 ttcgagtaat tagctcatca tttcttagaa cagttttccc cggacctaaa aacaaatctt
      481 ttaaaaatag agtcctctta gattgcttgc tctcaattag aacctgtgca tcaaaaacta
      541 tcaatgcagg tacagtatca gctgccggag aggcattccc tatattacct gctacggtac
      601 ccatatttct aatagctgga gcagctatgg cctgaattgc ttcatatagg gcggtatatt
      661 ctgttttaat tttatcttct ttaataatat gagaaaaagg aatagttgcc ccgattgaca
      721 atccattact ggtattaata aaatttagtt cggctatctc tttaatatac aggagataat
      781 caatgttaag ttctattgtc ttcatcttca ccagcaagtc agtcccccca gctaatatct
      841 taatattctt tttctgatat tcatccagta tatttagagc ctcagataaa cttttagggt
      901 ctaaatattc gaatttattt gctaatattc tggtattagt gtgcatcttt gttcttccct
      961 ccaattctat tcttatcttg caaggctttt aacatcactt caggggtgat gggaagcttg
     1021 tgaaaacgaa ttcctactgc atcatatact gcattaccaa aagaagatgc cactgcgctc
     1081 aaggcagcct cgccaatccc tttggcacca aaaggaccgg ttggctcata ggtatgggca
     1141 tgttctacgg taatattatc agtatggggc atatcagtag taatgggtac tttaaaatcg
     1201 gtaattaagc cattgcagtt caatttacca gattctttat tatatggtat gtcctctaat
     1261 tttcctaaac cgatacctaa ggcataagca cccagtatct gacctttcca taattcaggg
     1321 ttaataatgg tgcctgaatc acccatcatg accactctgg gtactgaaat ttcacccgtt
     1381 tctgtattaa cttcaacctc agtaaagtaa gtaataaaac agggggggca atttttctgt
     1441 cttaaggtag aagtataaac aaaagtacct acactattaa ttcgggcatg gtcagcaatc
     1501 tcggca
//
LOCUS       DAOQWT010000438         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_105306_length_1506_cov_2.833908, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000438 DAOQWT010000000
VERSION     DAOQWT010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105306_length_1506_cov_2.833908"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PKJ95_07635"
     CDS             complement(<1..999)
                     /locus_tag="PKJ95_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNR66144.1"
                     /translation="MSGGSIVMFIFNIFAALIIAILGIIVGLFYKGIDRKLAAMLQSR
                     VGPPIRQPFLDFFKLMIKENIVPENAVPWVFNGAPVLALVSTITILLYIPFGPIPALL
                     GNSGDLILIAYLLAIPAVALVSGGFSSGSTYASIGAQREMVLMMSYELPLVTTIIVLG
                     WRLNLAYPGLKVFSLAALSANPIWGLIGPLGFFGALLLLFTLVIVTPAELSIVPFDIP
                     EAETELAGGVLVEYSGRNLALFELANAVKMIVMGALTIALFFPYNIAHLFSITGFFAC
                     IINILFFFFKLFVVIFFEATFIRVALARLKIDQASTAYLIILSAASLMGLLLVAIDY"
     gene            complement(996..>1506)
                     /locus_tag="PKJ95_07640"
     CDS             complement(996..>1506)
                     /locus_tag="PKJ95_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH dehydrogenase subunit"
                     /protein_id="HNR66145.1"
                     /translation="DMDIKAITPDIYSGEVNGDVYDRIIVRILEVFQSVQIIEYCINN
                     LPEGEILSEPKIVKLLGTLKKAKGEGVGRHEAPRGEVIHYIKLNESENPEVWKARAPT
                     YNNLMSWLPMLQMQEIADIPIVIASIDPCIGCMDRVTLVDPGSNKKQVLTREELVKLS
                     IEKTRRMVR"
BASE COUNT          514 a          326 c          249 g          417 t
ORIGIN      
        1 gtaatcaatt gctactaaca gtaaacccat caaacttgct gcagaaagaa ttattaagta
       61 agctgttgaa gcctgatcaa tctttaaacg cgctaatgcc acacggataa aggttgcttc
      121 aaagaatata actacaaaca atttaaagaa aaagaacaaa atattgataa tacaagcaaa
      181 aaaaccagtt atagaaaata gatgtgctat attatatgga aaaaacaaag caattgttaa
      241 agctcccatt acaatcattt ttactgcatt tgccagttca aataaggcta aatttctacc
      301 ggaatactct actaagacac ctccagccag ttccgtttct gcctcaggta tgtcaaaagg
      361 aactatagat agttctgctg gggttactat taccagggta aagagcaaca ataaggcacc
      421 aaaaaaacct aacgggccaa ttaatcccca gatgggatta gcacttaaag ctgctaaaga
      481 aaaaaccttc aacccgggat aagctaaatt taatctccag cccagaacaa taatagtagt
      541 aaccaggggg agctcatagc tcatcattaa aaccatctct ctttgtgctc ctatactggc
      601 ataggtagag cctgaagaaa aaccgcctga taccagggca accgccggta tagctaatag
      661 ataggctatt aaaatcaaat caccagaatt tcctaataac gctggtatag ggccaaaagg
      721 aatatagagt aaaatagtaa tggtagaaac aagcgccagg actggtgcac cgttaaaaac
      781 ccagggaacg gcattctctg gtactatatt ttctttgatc attagcttga aaaaatctaa
      841 aaaaggttgc ctgatgggag gtcctacccg tgattgcagc atagcagcca gtttacggtc
      901 aatgccttta taaaacagtc ctactattat gcccagtata gcaattatca aggcagcaaa
      961 aatattgaaa ataaacatta caatacttcc cccgctcatc ttaccatcct cctggttttt
     1021 tcaatactta actttactaa ttcttctctg gttaatacct gtttcttatt ggaaccagga
     1081 tcaaccagag tcaccctatc catacagcca atacaggggt caatagatgc aataactata
     1141 gggatatcag caatttcctg catttgtagc attggcaacc aggacattaa attattatag
     1201 gtcggcgccc ttgccttcca tacttccgga ttttcggatt catttaattt tatataatgg
     1261 ataacctcac cccgcggagc ttcatgcctg cccactcctt caccttttgc ttttttcaat
     1321 gttcctaaca gtttgacaat ctttggttct gacaaaattt ctccctcagg caagttattt
     1381 atacaatatt ctataatttg aacagattgg aaaacctcca gtatacgtac aataatgcgg
     1441 tcatagacat caccatttac ttcacctgag taaatatcag gagtaatcgc ctttatatcc
     1501 atatca
//
LOCUS       DAOQWT010000439         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_106838_length_1493_cov_3.178720, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000439 DAOQWT010000000
VERSION     DAOQWT010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106838_length_1493_cov_3.178720"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKJ95_07645"
     CDS             complement(<1..214)
                     /locus_tag="PKJ95_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66146.1"
                     /translation="MAFLFFCILLPASAQQAGDVLTEEVPEGYVFYRYQYAYLDISLI
                     YPGKWLNYSSQERGILINTPDSQGSIM"
     gene            complement(283..>1493)
                     /locus_tag="PKJ95_07650"
     CDS             complement(283..>1493)
                     /locus_tag="PKJ95_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLAT/LH2 domain-containing protein"
                     /protein_id="HNR66147.1"
                     /translation="SNGGRVLSSNIPIFVSETEKGSDIDSDGDGILQEWEDEAMEYIN
                     PYIELDEEEDWLTKRDTDHVANYVRIHPSNSTGRSTISYHSENLPHYIIFRYVVTWSQ
                     DYGRFGVTAHKGDHERIFMAWKVIDNKNLRLEWVFKSSHEDPSAHHGVWNAWHRVCNK
                     GDVALNFTQKMYSEVMCSNLEFYNNRLLVYASEGKHAIYPSCDLCENVNLFIGVAGED
                     CGGGGTFRFDCYNVGEPPEFIDPAIYDLSLDRGKLEKELPEIDFKGFADKLSSRYRIQ
                     IKTGNEDLSGTDAKISIKLFGSEGTSSWYDVYSKPSPPLDITIRHLGTFEKGDTDNIY
                     VNSSDLGEINKIQIKHDNTGPGPGWLISEILVEDLVTHATWHARPNTWLDKFPWQDNT
                     NKTFNLSSTP"
BASE COUNT          461 a          321 c          232 g          479 t
ORIGIN      
        1 tcattattga accttgagaa tcaggagtat taatcaaaat tcctcgctcc tgggaagaat
       61 aattgagcca ttttcccgga taaatcaaag agatgtccag ataggcatat tggtatctgt
      121 agaaaacgta tccttccggc acctcctctg ttagaacatc tccagcttgt tgagccgaag
      181 ctggtagaag aatacagaaa aacaggaaag ccatataaaa aaagaaaata tttttttcat
      241 ctcttctctg ctcctacttt tagtatttag tattgagatt gcttaagggg ttgaacttaa
      301 attaaaagtt ttgttagtat tatcctgcca tggaaattta tccagccagg tattcggtcg
      361 agcatgccag gtagcatggg tcactaaatc ttctactaaa atttcactga taagccaccc
      421 cggacccgga ccggtattgt catgtttaat ttgaatttta ttaatctcgc ccaaatcaga
      481 tgaattcaca taaatattat ctgtatctcc tttctcgaag gtacctaaat gcctaattgt
      541 aatatcaaga ggagggcttg gtttagaata aacatcatac caggaagacg ttccttcgct
      601 tccaaaaagt ttaatgctta tcttggcatc agttcctgac aaatcttcat tgccagtctt
      661 tatttgtatt ctataacgac ttgagagttt atcagcaaat cctttaaaat caatttccgg
      721 caactctttt tctaattttc ctctatccag gcttaaatca tagatagcgg gatcgataaa
      781 ttcaggaggt tctccaacat tatagcaatc aaacctgaaa gttcctccac caccgcaatc
      841 ttcaccagct acacctataa ataaattgac attttcacac aaatcacaag atggatagat
      901 ggcatgttta ccttccgatg catataccag tagcctgtta ttataaaatt ccaagttact
      961 gcacataact tcagaataca ttttttgtgt gaagtttaag gcaacatcgc ctttattaca
     1021 taccctgtgc caggcattcc aaaccccatg atgagcacta ggatcctcat gtgaagattt
     1081 aaatacccat tctaatctca aatttttatt atctattacc ttccaggcca taaaaatacg
     1141 ttcatggtct cccttatgag ccgtgacacc aaatcgacca taatcttgac tccaggtaac
     1201 cacatagcga aaaataatat agtgtggaag attttctgaa tgatagctaa tggttgacct
     1261 tccagtagaa ttagatggat gtattcttac ataatttgcc acatggtctg tatctctttt
     1321 tgttaaccaa tcctcttctt catcaagttc aatataagga ttgatatact ccattgcttc
     1381 atcttcccat tcttgcaata ttccatcacc atctgaatca atgtcagaac ctttttccgt
     1441 ttcactcaca aaaatgggaa tgttactact aagtaccctg cctccattag ata
//
LOCUS       DAOQWT010000440         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_107302_length_1490_cov_1.697561, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000440 DAOQWT010000000
VERSION     DAOQWT010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107302_length_1490_cov_1.697561"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /gene="nadE"
                     /locus_tag="PKJ95_07655"
     CDS             <1..680
                     /gene="nadE"
                     /locus_tag="PKJ95_07655"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNR66148.1"
                     /translation="KKVRTAGKKGVIFGLSGGLDSAVLAVLCKRAFPDNTTALILPCE
                     SIENDLIDAMQITNKYHIDYQQFDLTPLYRQIISITNTQNFNNKMAKANIKPRLRMIM
                     LYYFASIHDYLVVGASNKSEISIGYFTKYGDGGVDILPLGNIVKSEIFEIARFLEIPE
                     NIISKPPSAGLWENQTDEQEMGFSYEQLDTFLKYGTLKNKNIERVILAMNRLSTHKRK
                     RPPAPEI"
     gene            838..>1490
                     /locus_tag="PKJ95_07660"
     CDS             838..>1490
                     /locus_tag="PKJ95_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNR66149.1"
                     /translation="MSGHSKWSTIKRKKGKADAERGKLFGKIIRLISIAARHGSDPET
                     NADLRNAIQLARENNMPSENIERAIKKGTGELEGVSYEEVIYEGYGPGGVAVLVEVVT
                     DNRNRTVSEIRRIFSRNGGNLGSTGCVAWIFSKMAYFSFDREKICQDKILDLALTVEV
                     DDIEIDDKNIEVNLPTSEFEKFKKLLEDNQVEYTLAEITMIPQSTVDLDGKKAEQML"
BASE COUNT          541 a          212 c          308 g          429 t
ORIGIN      
        1 agaagaaagt taggactgct gggaaaaaag gtgtcatttt tggtttaagc gggggattgg
       61 attcagcagt gcttgcagtt ttgtgtaaaa gagcttttcc tgataacacc acggctttaa
      121 tattgccctg tgaaagtata gaaaatgacc tcatagatgc tatgcaaatt accaacaaat
      181 accacatcga ttaccaacaa tttgacctta ccccattata tagacaaata atatcaatta
      241 ccaacaccca aaactttaat aataagatgg ccaaagctaa tattaagcca agattgagaa
      301 tgataatgct ttactatttt gccagtattc atgactattt agtggtagga gcaagtaata
      361 aaagtgagat atctatcggg tattttacta aatatggaga tggaggtgtt gatatattac
      421 cattgggaaa tattgtaaaa agtgagatat ttgagatagc tcgattttta gaaattccgg
      481 aaaatataat ttccaaaccc ccttctgccg gtttatggga aaatcagacc gatgaacaag
      541 agatgggttt tagctatgaa caactggata cctttctaaa atatgggaca ttaaaaaata
      601 aaaatataga aagagtcatt ttagcaatga atcgcttaag tactcataaa cggaaaaggc
      661 ctcctgctcc agaaatataa accggaaaat ggatactcag aataagagta attaaggaaa
      721 taaaatcata tcatatatca tacttgcatg ttttcgttta ctggtataat tcattcaata
      781 attgaaacgg tttttttatt atgctaaaat aagaaaaagg ttaaagggga ttttcttatg
      841 tcaggacatt caaagtggag taccattaaa agaaaaaaag gtaaagctga tgctgaaagg
      901 ggtaaattat ttggtaaaat aatccgactt atttctattg cagcccgcca cggttctgat
      961 ccggaaacga atgccgattt acgtaatgca atacaattag ccagagaaaa taatatgcct
     1021 agtgaaaaca ttgaacgagc aattaaaaaa gggactggtg aactggaagg tgtgtcatat
     1081 gaagaagtta tttatgaagg gtatggtcct ggaggtgtgg cagtacttgt ggaggtagtg
     1141 actgataatc gcaatagaac agtatccgaa attagaagga ttttttccag aaatggtggg
     1201 aatcttggct ctacaggttg cgtagcctgg attttttcaa agatggctta tttttcattt
     1261 gacagagaga agatatgcca ggataagata cttgatctgg ctttaacagt agaggttgat
     1321 gatattgaaa tagatgacaa aaatattgag gttaacctgc ctacctcaga atttgaaaag
     1381 tttaaaaaat tattggaaga taaccaggta gaatacacct tagccgagat taccatgatt
     1441 cctcaatcta cggtagattt ggacggaaaa aaagctgaac agatgttaaa
//
LOCUS       DAOQWT010000441         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_107813_length_1485_cov_2.651748, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000441 DAOQWT010000000
VERSION     DAOQWT010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107813_length_1485_cov_2.651748"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKJ95_07665"
     CDS             complement(<1..231)
                     /locus_tag="PKJ95_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66150.1"
                     /translation="MPRHKLPQELKSKHGSVSNVIRISLNKSKEDELEEYYTNKAETY
                     SELRKAQQKVIERQEKEEQVCISQKEEKYPTIS"
     gene            434..1168
                     /locus_tag="PKJ95_07670"
     CDS             434..1168
                     /locus_tag="PKJ95_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNR66151.1"
                     /translation="MKEEWMEKKDSFEVIDVRKLTSNFLPMILMKAKRKNVGEGLCVV
                     QTFEPKPLYSALEDLGYEYSTEKISEREYRAYFYRVKQKDITYESGADMPFKPTAILN
                     YKKVDDVLAENVVNFWELVWEKKDAAIDQKTKLLLSMANAVGASRFRQATRELIKAYS
                     LGVTIAELDEIFTLFAWNQGIGHFSSEIGPSVLFGVYRHIKNREKVGTDKKTIIDEVM
                     EMFGDRNPEVSVQFSYRKGKPCPKYP"
BASE COUNT          438 a          192 c          352 g          503 t
ORIGIN      
        1 tgagatagta ggatacttct cctctttttg ggaaatgcat acttgctcct ctttttcctg
       61 tctttcgata actttttgtt gtgctttgcg gagctcagag taggtttcgg ctttatttgt
      121 atagtattct tctagttcat cttcttttga tttattgaga gatattctga ttacattgga
      181 aactgagccg tgttttgatt ttaattcctg gggtagttta tgtcttggca tttatatcac
      241 ctcttcatat gatattatca tcacacttaa agatatttgt caaaaaagag ttgtggggat
      301 ggttgggtgg tgtcggtaat tgattggctt ttctggtata atagcactgt tatgtgaaaa
      361 tagtggagag taacaataat ttgaagatgt ttatagtata aatctatatc attaccataa
      421 ggagatggag agtatgaagg aagaatggat ggagaaaaaa gattcatttg aagtaattga
      481 tgtgaggaag ttaaccagta attttttacc tatgatatta atgaaggcta agcgtaagaa
      541 tgtcggggaa ggtttgtgtg tagtacagac ttttgaaccc aagccacttt attcagccct
      601 ggaagacctg ggctatgaat atagcactga aaagatatcg gaaagagaat atcgggcata
      661 tttttataga gtaaaacaga aagatattac ctatgagtcc ggtgcagata tgccttttaa
      721 acctactgct atactgaatt ataagaaagt agatgatgtt ctggcagaaa atgtggtgaa
      781 tttttgggaa cttgtctggg agaagaagga tgcggcaatt gaccagaaga ctaaattgct
      841 tttatcgatg gctaatgctg ttggtgcttc ccggttcaga caggcgacca gagaacttat
      901 aaaagcatat tccttagggg tgacgattgc agaacttgat gagatattca ctctttttgc
      961 ctggaatcag gggattggcc atttttcatc agaaataggt ccttcggttt tgttcggggt
     1021 gtatcggcac attaaaaacc gggagaaggt gggaacagac aagaagacta taatcgatga
     1081 ggttatggag atgtttgggg atagaaatcc tgaagttagt gtacaattca gttatagaaa
     1141 agggaaacca tgcccgaaat atccttaaag tggagtaagg gtaaatgtgg gaataagata
     1201 gtcgttggta tttagtcgtt ggttataaag tttttggttt atggtttttg gtttttagta
     1261 aaaatacaaa tactaaagat gtggggataa gtgaggaatg tttatatttt ttggtttata
     1321 ggatgttgag ggacggtcct ttaacatctt ttcaattcgt ttttttcagc tgacaattaa
     1381 aattttttcc ttgtcacaag ataaatacct ctctttgtca ctgtaagcaa tgaagttacg
     1441 aagtagtctc atataattat gtcgggcgat tggaatcatt ctgga
//
LOCUS       DAOQWT010000442         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_108396_length_1480_cov_4.493333, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000442 DAOQWT010000000
VERSION     DAOQWT010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108396_length_1480_cov_4.493333"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..747)
                     /gene="nagB"
                     /locus_tag="PKJ95_07675"
     CDS             complement(22..747)
                     /gene="nagB"
                     /locus_tag="PKJ95_07675"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNR66152.1"
                     /translation="MNIVIVKDYYELSIQAAQIVAAQINKIKKSVLGLPTGQTPLGMY
                     NELIKKYEKDEIDFSQVITFNLDEYYGLSPLHPQSYHYFMWNTFFQHINIKKENVNML
                     NGITENIDEECQRYENIIQIRGGIDLQLLGIGENAHIGFNEPANVLKSRTHLVNLSNS
                     TVKANSRFFNDIKEVPRQALTMGMGTIMKAKKIVLLASGKNKAPALAKTVNGLVSTRV
                     PASLLQLHKEVTILVDEDAASRI"
     gene            complement(786..>1480)
                     /locus_tag="PKJ95_07680"
     CDS             complement(786..>1480)
                     /locus_tag="PKJ95_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphofructokinase"
                     /protein_id="HNR66153.1"
                     /translation="TDKYVILQTVGRNTGWLAASAALVKDRESDAPHLIYIPERPLSK
                     DRFLAEVKECIKEYGWVSIVCGEGIQWEDKSFIANLHLNNDYANFEFGAMGGGSAAFN
                     LHKLIYQETNFRGEFQITESLSMCAIDRASKIDLDEAYCCGVKAIRIAEEDVSGVMVS
                     IVRTTSDPYQVELGVVPLEEVAVKIKPMDHKYINSQGNFVTDEYLKYLRPLVGELPGY
                     AELNFIKYPQLF"
BASE COUNT          439 a          308 c          230 g          503 t
ORIGIN      
        1 ttctaaaaaa ggaagggata attagatccg acttgccgca tcctcatcca caagtatagt
       61 tacttcctta tgaagctgga ggagacttgc cggtactcta gtactcacca gcccattaac
      121 agtcttagca agggcaggag ctttgttttt gccgctagct aaaagaacaa tttttttagc
      181 tttcataatt gtgcccatac ccatggtaag agcttgtctt ggaacttctt tgatatcatt
      241 aaaaaatctg gaattggctt tgacagtaga atttgacaga ttaaccaggt gagtcctgct
      301 ctttagtaca tttgccggct cattaaaacc gatatgggca ttttcaccaa tacccaataa
      361 ttgtaagtct atacctcctc ttatttgaat gatattttca tatctctggc attcctcatc
      421 aatattttca gttattccat tcaacatatt tacattttct tttttaatgt ttatatgctg
      481 aaaaaaagtg ttccacataa aatagtggta actttgtgga tgcaacggtg ataagccata
      541 atattcatcc aggttaaaag taatcacttg ggagaaatct atttcatctt tttcatattt
      601 tttaattaat tcattataca tacccagcgg tgtttgaccg gtcggcaaac ctaaaacgga
      661 tttttttatc ttgttaattt gagcggcaac tatctgcgca gcttgtatgc tcagttcata
      721 ataatctttt acaattacaa tgttcataaa ttacatctcc ttatagctta agccatccag
      781 ttaaactaaa ataattgagg atattttata aaatttaatt cagcatagcc tggtaactct
      841 cccaccagag gtctgaggta ttttaggtat tcatcagtta caaagtttcc ttgagaattg
      901 atatatttat ggtccatggg ttttatcttc actgctacct cttcaagggg aacaacaccc
      961 agctctactt gataaggatc agaagtggtg cggactatag aaaccattac ccctgataca
     1021 tcctcttcag caatcctaat tgctttaaca ccacagcaat atgcttcatc cagatcgatt
     1081 ttactcgctc gatctatggc acacattgat aatgattcag ttatctgaaa ttcccctctg
     1141 aagttagttt cctgatatat aagcttgtgc aggttaaatg ctgcacttcc tcctcccata
     1201 gctccaaatt caaaattagc ataatcatta ttcaaatgga gattggcaat gaaactttta
     1261 tcttcccact gtataccctc accacagaca attgataccc aaccgtattc tttgatacat
     1321 tctttaacct ctgctaaaaa tctatccttt gataaaggcc tttctggtat ataaataaga
     1381 tgaggagcat cagattctct atctttaacc agtgctgcag aagcagctaa ccatcctgtg
     1441 tttcttccca ctgtttgtag aataacatat ttatcagtac
//
LOCUS       DAOQWT010000443         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_108429_length_1480_cov_2.910877, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000443 DAOQWT010000000
VERSION     DAOQWT010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108429_length_1480_cov_2.910877"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    217..226
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <227..761
                     /locus_tag="PKJ95_07685"
     CDS             <227..761
                     /locus_tag="PKJ95_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNR66154.1"
                     /translation="DTDLEVMVKVLQVGIDKIESKGIKSIRSRVTNIKPYFNQQITIE
                     VFRESILKELFQGKEVNYYYLDEKDLKEINNLVNTKYSTWEWNFGYSPSYSIKNSRRF
                     PAGKIELFIDVEKGMIKSCQIFGDFLSLCDISEIEKALQNKCFEPEVIEKTLVQFNLK
                     HYFGEITLPEIMTVFFG"
     gene            943..>1480
                     /locus_tag="PKJ95_07690"
     CDS             943..>1480
                     /locus_tag="PKJ95_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HNR66155.1"
                     /translation="MSQTLFKVRTIEDSLKIFRESFNFVKFFKNREQLIELIDAYGRV
                     LAEDIVSSENIPGFNRAMVDGFAVKASDTYGASDSLPAYLELVGEIKMSERPSFLLQS
                     GQAVKISTGGMLPEGADAVVMLEYTENLNPTLVEMSRSVASWENVLREDEDIKKEEIV
                     LKKGHQLRPQDIGLLAALG"
BASE COUNT          484 a          219 c          326 g          441 t
ORIGIN      
        1 gtaatttaaa tttcactttt atcaagtgcc tggatgaggc ttcgaatttg gatattaaaa
       61 aatttgcttt accggtagtg gaagcattga ataaattggg tgttcctgct caattaacgg
      121 ggcgtaatga tataagtgtg gaaaataaaa aaatatccgg taatgcccag cgcctctata
      181 gaaacaagct cctccaccat ggaaccctac tttttgnnnn nnnnnntgat acagatttag
      241 aggtaatggt aaaagtgcta caggttggaa ttgacaagat agaatcaaag ggaattaagt
      301 ccatacgtag tcgcgttacc aatatcaaac cctattttaa tcagcagatt accattgaag
      361 tatttcggga atcaattcta aaggaattgt ttcagggcaa agaggttaat tactattatt
      421 tagatgagaa agatctaaaa gaaataaata atctggtcaa taccaaatat agtacctggg
      481 agtggaattt tggctactcc ccatcttata gtatcaagaa ttctcggcga ttcccggctg
      541 gcaaaataga actttttatt gatgtagaaa aggggatgat taagagctgt cagatattcg
      601 gtgatttttt atcattatgt gatatttctg agattgaaaa ggcattacag aataagtgtt
      661 ttgaaccgga ggttatagaa aaaaccctgg tgcagttcaa tctgaagcat tattttggag
      721 aaataacatt accggagata atgacagtct tttttggtta attaacatta ccaaactatg
      781 ttaatgtaaa aaatattact ttattttaat ttttataaga acagagttat ttttttgaaa
      841 agatagagca atttgattaa aatgttgtgg ccaggcttta atattctgga atatgtttaa
      901 taagagagga ttttcagggg tatttctcct gatgacactt ctatgtctca aacgcttttt
      961 aaggtaagaa caattgaaga tagtctaaaa atatttcgtg aatcttttaa ttttgtaaaa
     1021 ttctttaaaa accgtgagca attgatagaa cttattgatg cttacggaag agtgctggca
     1081 gaagatattg tgtcttctga gaatattcca gggttcaaca gggcaatggt ggatggattt
     1141 gccgtaaaag ccagtgatac ctatggagca agtgacagcc taccagctta tctcgagctg
     1201 gttggagaaa taaaaatgtc agaaagacct tcttttctgt tacagagcgg acaggcagtg
     1261 aaaatatcca ccggggggat gttgcctgaa ggggctgatg cagtggtaat gttagaatat
     1321 accgagaacc tgaatcccac cctggtagag atgagccgct cggtcgcttc ctgggaaaat
     1381 gtattgcgag aagatgaaga tattaaaaaa gaggagatcg tattaaaaaa gggccatcag
     1441 ctgaggccac aggatatagg gctactcgct gccctggggc
//
LOCUS       DAOQWT010000444         1479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_108562_length_1479_cov_2.819522, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000444 DAOQWT010000000
VERSION     DAOQWT010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108562_length_1479_cov_2.819522"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /gene="ricT"
                     /locus_tag="PKJ95_07695"
     CDS             <1..459
                     /gene="ricT"
                     /locus_tag="PKJ95_07695"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNR66156.1"
                     /translation="LGICGRPLCCSIFLTEFKPVPINVAKMQSVALNLSKISGTCGRL
                     KCCLNFEAEFYQEELKNYPKLGSQVKTREGKEGKVTEVNILSRYVVIETVEKDDNESI
                     KNRIKLPIEEWQRNYNKMNRDVPNAENKSASGRDNRTTGKISIESSPEQE"
     gene            377..1069
                     /locus_tag="PKJ95_07700"
     CDS             377..1069
                     /locus_tag="PKJ95_07700"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNR66157.1"
                     /translation="MPKINLLPGEIIEPLGKYQLKVLQNRNSYCFSVDSILLAEFTKI
                     KNREKIVDLGTGCGIIPLLLYHPEKNNLIYGVEIQEKLVSLARRSVTINNLDEHIFIL
                     QEDINNLKENFPGEYFDVITVNPPYIPGGRGKSSSDHEQLIARHEININLDNMLKMSN
                     YLLKKRGRLYLIHRADIFVPVIMALKKYSLEPKILQFVYTKKDKEAKRFLCEARKEGG
                     TELKVLTPIFLE"
     gene            1357..>1479
                     /locus_tag="PKJ95_07705"
     CDS             1357..>1479
                     /locus_tag="PKJ95_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNR66158.1"
                     /translation="MKKQKSGKLYICGTPIGNLEDITLRCLAILKKVDLIAAEDT"
BASE COUNT          553 a          207 c          283 g          436 t
ORIGIN      
        1 cttggtatct gtggtagacc attatgctgt tcaatttttt taacagaatt taaaccggtg
       61 cccataaatg tggcaaagat gcagagtgta gctctcaatc tttccaaaat ttccggtacc
      121 tgtggcagat tgaaatgctg tcttaacttt gaagccgaat tttatcagga ggaattaaaa
      181 aattatccca aattgggcag tcaggttaag accagagaag gtaaagaagg aaaggttacc
      241 gaggttaata tattaagcag gtatgtggtc attgaaaccg tagaaaaaga tgataatgaa
      301 agtattaaaa atagaattaa gttacctata gaggaatggc aaagaaatta taataagatg
      361 aatagagatg tgcctaatgc cgaaaataaa tctgcttccg ggagagataa tcgaaccact
      421 gggaaaatat caattgaaag ttctccagaa caggaatagc tactgctttt cagtggattc
      481 tatcctgctg gcagaattta caaaaattaa aaacagggaa aagattgttg atctgggtac
      541 cggttgtgga attatccctc tcctgctcta ccaccctgaa aaaaataatc ttatttatgg
      601 ggtagagata caggagaaac tggtctctct tgccagaaga agtgtaacta taaataatct
      661 ggatgaacat atctttattt tgcaggaaga tataaataat ttaaaagaaa attttccggg
      721 tgaatatttt gatgtcatta ctgtcaatcc gccctatatt cccggtggca gagggaaaag
      781 cagttctgac catgaacaat taatagcccg ccatgaaata aatataaacc tggataatat
      841 gttgaaaatg agtaattacc ttctgaaaaa aaggggcaga ctatacctta tacacagggc
      901 ggatattttt gtacctgtta taatggcgtt aaagaaatat tctttagaac caaagatatt
      961 gcaatttgtt tataccaaaa aagataaaga agctaaaaga tttctctgcg aagcaaggaa
     1021 agagggaggg accgagctga aggtcttaac ccccattttc ttagaataaa tattaaaata
     1081 gatgttatat attattatag agaatagatg tagacaggtc cggagataat cggtaaacaa
     1141 tcataatgaa agttgttatt aatggttttt agttcatagt tttttgtttt tggtaaaaat
     1201 aatattggaa taagattttt ttaaataaaa ataacatttt aaaaaataat caataatgat
     1261 tcaagaaaat tcaagaaaat aatttaaata aaccttctca aacagggtta tactaaaaac
     1321 tataaacaat aaactataaa ctttttaatg gtttttatga aaaaacaaaa atccggtaaa
     1381 ttgtatatct gcggtacccc gattggcaat ctggaggata tcaccctgcg ttgtctggca
     1441 attttaaaaa aagtggattt gattgcagct gaagatacc
//
LOCUS       DAOQWT010000445         1474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_109170_length_1474_cov_2.393235, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000445 DAOQWT010000000
VERSION     DAOQWT010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109170_length_1474_cov_2.393235"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKJ95_07710"
     CDS             complement(<1..199)
                     /locus_tag="PKJ95_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNR66159.1"
                     /translation="MEITVLTIFPEMFNSPFSESIIKRAKDKNLVNINIIDIREFTKD
                     RHKSVDDYSYGGGPGMVLKPEP"
     gene            complement(241..483)
                     /locus_tag="PKJ95_07715"
     CDS             complement(241..483)
                     /locus_tag="PKJ95_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNR66160.1"
                     /translation="MRETIDYLLKKLVDFPDKLKVDKKKLDNVTILEITAEPADIGKI
                     IGKEGRVIKAIRAIVSAATIKEKNKTIIEIIESKNQ"
     gene            complement(540..800)
                     /gene="rpsP"
                     /locus_tag="PKJ95_07720"
     CDS             complement(540..800)
                     /gene="rpsP"
                     /locus_tag="PKJ95_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNR66161.1"
                     /translation="MSARIKLRREGAKKNPCYKVIVIDSSKTGDGRYIEMLGYYQPTK
                     EPYILNINKEMSLKWVKQGAKMTKTVESLFKKEGILDNSING"
     gene            complement(836..>1474)
                     /locus_tag="PKJ95_07725"
     CDS             complement(836..>1474)
                     /locus_tag="PKJ95_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNR66162.1"
                     /translation="ANDFNNEVGADGFILTKLDGDARGGVALSLKCRTNLPIKFVGIG
                     EKITDLEPFYPERISSRILGMGDTLTLIEKIESNYARNELQNMGKKAFREQFNLNDFY
                     EQFKKIKSIGSLDNILNLMPFKFSNSLKNMKEELGDEEKGFRRVEAILCSMTRQEKID
                     PDIINGSRRRRIAKGSGTTVSEVNRLINQFFKMKKFLKHQVGLDSLIPFMRK"
BASE COUNT          428 a          276 c          176 g          594 t
ORIGIN      
        1 taggttccgg ctttagaacc attccaggac caccgccata ggagtagtca tcaacacttt
       61 tatgtcggtc tttggtaaat tctcttatat ctataatatt gatatttact aaatttttat
      121 ctttagccct tttaataatg ctttctgaaa aaggtgaatt aaacatctct ggaaaaattg
      181 tcaaaacagt tatttccata gttctcctta tgcattttac aagaattaaa aggcaaataa
      241 ttattggttt ttactttcta ttatttcaat tatggtttta tttttttctt ttatagttgc
      301 tgcactaact attgccctaa ttgccttgat tactcttccc tctttaccaa taatttttcc
      361 aatatctgca ggctcagctg taatttctaa tatagtaaca ttatcaagtt ttttcttatc
      421 aacctttagc ttatccggga aatcgactaa cttttttagt aaatagtcaa tagtttctct
      481 caatatattt ctccaaagat ttttggtaaa tttatactat aatttatttt tataaatttt
      541 taaccattta tagaattatc caggatgcct tcttttttaa acaaggattc gactgtttta
      601 gtcatttttg ctccctgctt tacccatttt aaactcattt ctttattgat atttaagata
      661 tatggttctt ttgtcggttg ataatatccc aacatctcta tatatcttcc atcacctgtt
      721 ttactggaat ctatcactat taccttataa caaggatttt ttttggcacc ttctcttctc
      781 agttttattc ttgcagacat tttttcctcc aaaaattaat tagaatattt aatcgttatt
      841 ttctcataaa aggaattaat gaatctaaac cgacttgatg ttttaaaaat ttcttcattt
      901 taaaaaattg attaattagc cgatttactt cactaaccgt tgtaccgcta cctttggcaa
      961 ttcttcttct cctactaccg tttattatat cgggatctat cttttcttgt ctggtcattg
     1021 aacagagtat cgcctcaact cgtctaaagc ctttttcttc atctcccaat tcctctttca
     1081 tattctttaa tgagtttgaa aatttaaagg gcatcaagtt taatatatta tccagagaac
     1141 caatactttt tattttttta aattgctcat aaaaatcatt aagattgaat tgttctctaa
     1201 atgctttttt ccccatattc tgcagctcat tacgggcgta gttagattca attttttcga
     1261 tcagtgttaa ggtatcaccc attccaagaa tacgagaaga aatacgctca ggataaaagg
     1321 gttctaaatc tgtaatcttt tcgccaatac caacaaattt aataggtaaa tttgttctac
     1381 attttaagga cagagcaaca cctccgcggg catcaccatc taatttggtt agaataaagc
     1441 catctgcccc tacttcatta ttaaaatcgt tagc
//
LOCUS       DAOQWT010000446         1472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_109356_length_1472_cov_8.722653, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000446 DAOQWT010000000
VERSION     DAOQWT010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109356_length_1472_cov_8.722653"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1184
                     /locus_tag="PKJ95_07730"
     CDS             132..1184
                     /locus_tag="PKJ95_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66163.1"
                     /translation="MGIISELSEKVNTTNLYCKCRICSRHLLENSIYSYYGYCFYHYP
                     IGESDGDDCFPGKLGYKDISGENREKLYTISNSHFENNTRNISSEIQFHTGDIIEQYQ
                     YEKPIVTGLQDVKRRKKKKKNNRNKKKHQDIENKREERRKQIQERSVNLDNLRRSRSR
                     KKKEIRRIVNSNCTNDNKKYCSFFTCTYKDNFLDEYKAREDINKFIKRLQYNYPGKEE
                     DGRFLDFTQYLWAVERQKRGSVHFHIIFFNCPYLHWSKYQEEWGKGTIDIHQVRYARN
                     AGSYVCKYFTDYDEQFFDNPIIGRTWGKSRNLKNSIIKRFSEYQGCLPCYKVLKTYTY
                     HTEINGYCLLRICKRE"
     gene            complement(1287..>1472)
                     /locus_tag="PKJ95_07735"
     CDS             complement(1287..>1472)
                     /locus_tag="PKJ95_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66164.1"
                     /translation="PAHVLPPRGLLNSTGQPNTAFSGTMSITHSSSYGIITTASMKFK
                     GNIFPIRSRTGKIFYYP"
BASE COUNT          510 a          193 c          304 g          465 t
ORIGIN      
        1 tataaaattg aatatgtcgg cttcgattgt ttaagatgta atttttgaga ggaaattatt
       61 aaactcccac ctgtaaagta ggagtttaga agttcgcctg gtgaaaacca acagctaact
      121 tattaaaagc tatgggtata atatcagaac taagcgaaaa agtaaatact actaatttat
      181 attgtaagtg caggatttgc agtcgtcatc ttttagagaa tagtatttat agttattatg
      241 gttattgttt ttatcattat cctataggtg agtcagatgg tgatgattgt tttcctggta
      301 aacttggcta taaggacata tctggagaaa atcgagaaaa gctatatacc ataagcaatt
      361 ctcattttga aaataacaca agaaatatat caagtgagat acaattccat actggtgata
      421 ttatagaaca gtaccaatat gaaaaaccca tagtaaccgg attgcaagat gtgaagagaa
      481 ggaaaaagaa gaagaaaaat aatcgtaaca aaaagaaaca tcaggatatt gaaaataaaa
      541 gagaagaaag aagaaaacag atccaggaga ggtctgttaa tttagataat ttgaggcgtt
      601 ccaggagtcg taaaaagaaa gaaattcgta gaatagtaaa ttctaattgc actaatgata
      661 ataagaaata ttgcagtttt tttacctgca cttataaaga taatttttta gatgaatata
      721 aagccagaga agatataaat aaattcatta agaggcttca atataattat cctggtaaag
      781 aagaagatgg aagatttctt gattttactc aatatttatg ggccgttgag cgtcagaaaa
      841 gagggtctgt tcattttcat ataatattct ttaattgtcc atatcttcac tggtcgaagt
      901 atcaagaaga atgggggaaa ggtactatag acattcatca ggttcgttat gctcgtaatg
      961 ctggcagtta tgtttgcaaa tatttcactg attatgacga acaatttttt gataatccta
     1021 ttattggtcg aacttggggg aagtccagaa atttaaaaaa ttctattata aagaggtttt
     1081 ctgagtatca aggctgtttg ccttgctata aagtattaaa gacatacaca tatcatactg
     1141 aaattaatgg ttattgtctt ttaagaattt gtaagcgtga atgagtgggt gatttttaag
     1201 gttctaaccg attgttatct tttccggttg tccgtctatc gctctttcgt cctctgtagg
     1261 cttcttaacg gaggcagcta tgaaaatcaa gggtaataga atattttccc tgtcctactc
     1321 ctgataggga aaatattccc cttgaatttc atagatgctg tagttatgat gccgtaggag
     1381 gatgaatgag tgatagacat tgtaccggaa aaggctgtat tcggttgacc ggttgagttc
     1441 aggagtcctc tgggaggtag cacgtgagcg gg
//
LOCUS       DAOQWT010000447         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_111479_length_1455_cov_3.607857, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000447 DAOQWT010000000
VERSION     DAOQWT010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111479_length_1455_cov_3.607857"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PKJ95_07740"
     CDS             <1..710
                     /locus_tag="PKJ95_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNR66165.1"
                     /translation="YNFEIQVFKKEKIIRPKTLGQKKILEKIGNNEILFVIGPAGTGK
                     TYLAVAIAISSLKKKEVDRIILVRPAVEAGESLGFLPGDLMEKIDPYFRPLYDAIYEM
                     MPSEKFQNFLERGTIEIAPLAYMRGRTLNNSFIILDDAQNTTLGQMKMFLTRFGFGSK
                     IIVTGDITQVDLPQKTNSGLVSIAEILKDINGISFVYLEKKDVVRHRLVKEIIHAYEN
                     ASKIDNKIIKNLERNNL"
     gene            711..>1455
                     /locus_tag="PKJ95_07745"
     CDS             711..>1455
                     /locus_tag="PKJ95_07745"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66166.1"
                     /translation="MRMVKLGRKNNKIFQKYFLQRLLFIFLIIIIMTVILSINFVPGK
                     ILLKAGEVAPRNISATETIEFVDKKATEKLKEEAAKSTHEVYNLNLASIENTERELLD
                     FFSDIRKLRNQYNLLIEERQIENTLNPDELINNSSINNGLGIVADKYLFDRGNPLLIN
                     LVKLDRSSLEEIEENVSLSLKKIMQQGVKENDIELAKKQIVREISELSTSPYTAILAG
                     EVGENFLKPSLFLDEETTLSKKQEAVAAVR"
BASE COUNT          559 a          182 c          266 g          448 t
ORIGIN      
        1 tatacaattt tgagatacag gtttttaaaa aagaaaaaat aattagaccg aaaacattag
       61 gacagaaaaa aattttagaa aaaataggta ataatgaaat attatttgtt attggacctg
      121 ctggaaccgg aaagacctat ctggcggtcg ctattgctat ttcttccttg aagaagaaag
      181 aagttgatag aataatctta gtccgtccgg ctgttgaagc aggggagagt cttggcttct
      241 tgccaggtga tttaatggaa aaaatagatc cctactttag gccattgtat gatgctattt
      301 atgagatgat gccttctgaa aaatttcaaa actttttaga acgcggaacc attgagattg
      361 ctccacttgc ctatatgaga gggagaactt tgaataattc ctttattatt ttagatgatg
      421 cacagaatac taccttagga caaatgaaaa tgtttttgac tcgttttggt tttgggtcaa
      481 aaattattgt aactggtgat attactcagg ttgacttacc ccaaaaaact aattctggtt
      541 tagttagtat agccgagata ttaaaagaca ttaatggaat ttcatttgtg tatcttgaaa
      601 aaaaggatgt agttagacat agattggtta aagaaattat ccatgcctat gaaaatgctt
      661 ctaaaattga taataaaatt ataaaaaact tagaaagaaa taatttataa atgagaatgg
      721 ttaaattagg cagaaaaaat aataaaatat ttcaaaaata ttttttacaa agattattat
      781 tcatttttct gataattatt attatgaccg ttattttatc aataaacttt gtacctggaa
      841 aaatattgct aaaagcagga gaggttgctc caagaaatat ttctgcaact gaaacaatag
      901 aattcgtaga taagaaagct actgaaaaat taaaagaaga ggcagcaaaa tcaacccatg
      961 aggtatataa cttaaattta gcaagtatcg aaaatactga aagggaattg ttggattttt
     1021 tttctgatat aagaaaacta agaaatcaat ataaccttct tatagaggaa agacaaattg
     1081 aaaataccct taatcccgat gaactgataa acaactcctc aattaataac ggattaggga
     1141 tagtggcaga taaatattta tttgatagag gaaatccttt attaattaat ttggttaaat
     1201 tagatcggtc ttccttagaa gaaattgaag agaatgttag tctttcctta aaaaagatta
     1261 tgcaacaggg tgttaaagaa aatgatatag aactggcaaa aaaacagatt gtaagggaaa
     1321 taagtgaatt atctaccagc ccgtataccg ctattttggc aggagaagtc ggggaaaatt
     1381 ttctgaagcc aagtctattt ttagatgaag aaacaacctt gtctaaaaag caagaagctg
     1441 tagctgccgt aaggg
//
LOCUS       DAOQWT010000448         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_112086_length_1451_cov_2.273639, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000448 DAOQWT010000000
VERSION     DAOQWT010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112086_length_1451_cov_2.273639"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="PKJ95_07750"
     CDS             <1..1043
                     /locus_tag="PKJ95_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNR66167.1"
                     /translation="ISGEAVDLCKVEGVDAVISAHFHAFVSGLVDGVPIVQANYNGRA
                     IARTVFTLDDDNRLISAEPSIDVLYERADTLQDDPTTLAIYEKYETDLEPILGRVLGE
                     TTVELDHDRYAGPSLLGEWACQVMQDKVGAQIAMTNGGGLRTPIPKGKITAGKLYEVL
                     PFDNTLYVLKISGADVKANIEHGIMNENVGWIAISGVRVVYDKDAEAGNRIVSMVLAD
                     GTAIEMDKYYTVVTNDFMATNGDNYNFSNAIEKKDTFLPVRDALMEAVEKAGVISPVR
                     QNWLSELSTPKTYIVVSGDSIWKIAQKFGTTMEKIIELNELPNPRLILPGQELVLPKV
                     IEKITCIKRWVV"
BASE COUNT          471 a          221 c          317 g          442 t
ORIGIN      
        1 atatcagcgg agaggcagta gatttatgta aggtagaagg agtagacgct gttatttcag
       61 cacatttcca tgcctttgtt agtggattgg tggatggtgt accgatagta caggctaatt
      121 ataatggtcg ggcaattgcc agaaccgtct ttactttaga cgatgataat cggttaataa
      181 gtgctgaacc ttctatagat gttctttatg aaagggctga tacacttcaa gatgatccca
      241 ccacactggc aatatatgag aagtatgaaa cagacttaga accaattctg ggaagggtgc
      301 ttggtgaaac tactgtggaa ttagaccatg accgttatgc cggaccttcc cttttaggag
      361 agtgggcctg tcaggtgatg caggataagg tcggtgccca gattgctatg accaatggtg
      421 gtggcttaag aactcctata cccaaaggca agattactgc cggcaaatta tatgaagtgt
      481 tgccttttga taatacgcta tatgttttaa aaatatccgg agcagacgta aaagccaata
      541 ttgaacacgg gattatgaat gagaatgttg gctggattgc catatcggga gtaagagtcg
      601 tttatgataa agatgctgaa gcaggaaatc gaattgtcag tatggtattg gcagatggta
      661 ctgctattga gatggataaa tattataccg tagtaaccaa cgattttatg gctactaatg
      721 gggataatta taatttctcc aatgccattg aaaagaaaga taccttcctg ccggttagag
      781 atgccctgat ggaagcggta gaaaaagcgg gagttatttc accggtaagg cagaactggc
      841 tgtcagaact gagcactcct aaaacctata ttgttgtctc tggcgattct atctggaaga
      901 ttgcccaaaa atttggtact actatggaaa agattattga actgaatgaa ttgcccaacc
      961 cccgattaat cctgcctggg caagaattag ttcttcccaa agttatcgag aaaataacat
     1021 gtataaaaag gtgggtggtc taagtttttt tattgatgac tgcccacttt tttatcagtt
     1081 tgtaaaatta tgatttattc cagaattagt taaaaatggt ttagattggg aaaaagattt
     1141 tttggaaaat ttaccgaata taaaaacaac accatgaata agtttgaggg aaaaacgaaa
     1201 aagatatgtt ataatgtaac atacttgttt ttttaaaata taatttaata aaaaaagaga
     1261 taatcaattt gctaaagagt gaaggaagaa tttaatgagc ttaaaaatat ttcaagtatt
     1321 tgcgctggcc tgtctggcag tatttacttt ctttatctct gattttcgca ataaaaaagg
     1381 gatgttaccg ctgttaaaca aaaaaatagt ctctattgta aaatactctt attttatacc
     1441 ggtttttatc t
//
LOCUS       DAOQWT010000449         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_112201_length_1450_cov_2.427240, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000449 DAOQWT010000000
VERSION     DAOQWT010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112201_length_1450_cov_2.427240"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PKJ95_07755"
     CDS             complement(<1..943)
                     /locus_tag="PKJ95_07755"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNR66168.1"
                     /translation="MESSNIVMHDKKVIFTPCETYLEDDINSALEKLFQELGGLNHFV
                     QTGQKVLLKVNLLMKKPPEAAVTTHPALVEAIINKIHALGAKVIIADSPGGPFTRSWL
                     KGIYHDTGMEEVAARTGAQLNWNFEQQEYPNPEGKILKHITLAKIIKEVDVVISMAKL
                     KTHGFTIYTGAVKNLFGTIPGLLKAEYHLRMSDVRDFCDMLVDVAQFVKPELSIMDAV
                     IAMEGAGPSAGDPKQVGLLAASTNPHALDLACCHLIGIEPKRVFTLQKAIDRGLIPMD
                     IKGLEIRGIDINNFKPIPFKVPITSRARHVPIPLPAKL"
     gene            1115..>1450
                     /locus_tag="PKJ95_07760"
     CDS             1115..>1450
                     /locus_tag="PKJ95_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNR66169.1"
                     /translation="MPFGYNGKILRVDLDQESYSIEQPDEIFYRTYWGGRGLGLYYML
                     KEMKPGVEPFSGDNLLIFSTSVIVGNGAPSIPRYTVCAKSPLTGAQGESEAGGYWGPE
                     LKKAGFDAIV"
BASE COUNT          423 a          292 c          307 g          428 t
ORIGIN      
        1 ccagttttgc cggcaatggt ataggcacat gtctggcacg ggaagtaata ggtactttaa
       61 agggaatagg tttaaaattg tttatatcta ttcccctgat ttccaggcct ttaatatcca
      121 tcggtattaa acctctatcg atagccttct gcagagtaaa aacgcgtttc ggttcaatac
      181 caattaggtg gcagcaggcc aaatcaagag catgagggtt ggtggaagca gccagcaacc
      241 cgacctgttt cgggtcaccg gcagatggcc cggctccttc catagctatt actgcatcca
      301 taatggatag ttctggtttt acgaattggg caacatctac cagcatatca cagaagtccc
      361 tgacatcact cattctcagg tggtattcag cctttaacaa gcccggaatg gttccgaaga
      421 gattcttgac tgcaccggta tagatagtga agccatgggt tttaagctta gccattgaaa
      481 ttaccacatc aacttctttg atgatcttag caagggtaat atgttttaga attttccctt
      541 ccgggttggg atattcctgc tgctcaaaat tccagtttag ttgcgcacca gtcctggcag
      601 ctacctcttc cataccggta tcgtgataaa tgccttttaa ccagcttctg gtaaaaggtc
      661 cccctggact gtcggctatt attacctttg ctccgagggc atgaatttta ttgataatag
      721 cttccaccag agcgggatgt gtggtaacag ccgcctcagg aggttttttc atcaataggt
      781 ttacttttag caaaaccttt tgcccagttt gtacaaaatg gtttaagcca cccagctcct
      841 gaaacaattt ttccagcgca gaatttatat catcctccag gtatgtttca cagggtgtaa
      901 aaattacttt tttatcatgc ataacgatat tactactttc caaaatttta acctatccat
      961 tataacaaaa atttgttaga ataaatagct gattagatta acttattaaa tgtgacagta
     1021 tatatcaaat aaaattttag aaaatagcac agcaatatgg attgaaggag gcaaataagc
     1081 ttaacaaaaa tattgctggg aaaggagtag ttctatgcct ttcggttata acggaaaaat
     1141 attaagagta gaccttgacc aggagagcta ttctattgaa cagcctgatg agatttttta
     1201 tcgcacctac tggggaggca gagggttagg actttactat atgctaaagg agatgaaacc
     1261 cggagtagaa ccattttcag gagataatct gctaatcttt tctaccagtg taatcgtagg
     1321 gaatggagct ccatcaatac ccagatatac agtctgtgca aaatcccctt taactggagc
     1381 gcagggagaa tctgaggcag gtggttactg gggaccggaa cttaaaaaag ctggttttga
     1441 tgccatagtt
//
LOCUS       DAOQWT010000450         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_112938_length_1444_cov_3.802016, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000450 DAOQWT010000000
VERSION     DAOQWT010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112938_length_1444_cov_3.802016"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PKJ95_07765"
     CDS             <1..835
                     /locus_tag="PKJ95_07765"
                     /inference="COORDINATES: protein motif:HMM:NF037377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66170.1"
                     /translation="MILYMLANGIGRFRGALKGTQKTSNWRTIAFSTGERRLSEVTEY
                     EGAKARIISLYGPPFKTEKQAELIYNIKSDIHANFGIAGPMFVEHIINKGYHESYKDL
                     REQYKNTALELAKLGKDNVSDRMSQYFAAVQIAGKMVEELFKFGGEPEKIVRTMFEYT
                     CGENKERGDYPQRALEHIVSWVQGNQNSFDRQDQLATVERGEIFGVIREGDYIGIFPH
                     KFKEIIKKSEFDNSSILKAFKERTWIQTTKNNFTCLVGFRSKVVRMIKILWPAINNIW
                     S"
     gene            complement(1176..>1444)
                     /locus_tag="PKJ95_07770"
     CDS             complement(1176..>1444)
                     /locus_tag="PKJ95_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66171.1"
                     /translation="NGMLLPLDISCNKSYSIAGPGSACSRSLSCFARCKHSSGSSYFC
                     NGADIQSCADRCNYCRLLWVGKGGAGPGKTSFCHFKYSCGIPNL"
BASE COUNT          531 a          220 c          274 g          419 t
ORIGIN      
        1 aatgattctc tatatgcttg ccaatggtat aggaagattc agaggagctc taaaaggcac
       61 tcagaaaaca tcaaactgga gaacaatagc tttctctaca ggtgaaagaa gactgagcga
      121 agtaacagaa tatgaaggag caaaagcaag aataataagt ctttacggac ctccttttaa
      181 aacagaaaag caggcagagc ttatatataa tataaaatct gatattcatg ccaacttcgg
      241 catagcgggc cctatgtttg tagaacatat aataaacaaa ggatatcacg aatcatataa
      301 agatctaaga gaacaatata aaaatactgc actggaactg gcaaaattag gtaaagataa
      361 tgtatcagat cgtatgtcac agtattttgc agcagtgcag atagccggga aaatggttga
      421 agagctcttt aaatttggtg gagagccgga gaaaatagta agaaccatgt ttgaatatac
      481 ctgcggagaa aataaagaaa gaggagatta cccgcaaaga gctttagaac atatagtttc
      541 atgggttcag gggaatcaga atagttttga taggcaagac cagctggcaa ctgtagaaag
      601 aggagaaatc tttggagtaa taagagaagg agattacata ggaatattcc ctcataaatt
      661 taaagaaatt atcaaaaaaa gcgaatttga taactcttct attctaaaag cttttaaaga
      721 aagaacctgg atacaaacca caaaaaacaa ttttacctgt cttgtaggat ttagaagtaa
      781 agtagtaaga atgataaaaa ttctatggcc tgcaattaat aatatatggt cataataaaa
      841 taaatatttt gtagaaaatt ttttttcata ttttccagta taaaaaattt ttttacacgt
      901 taaacacgtt atccataaat atatatataa taaattattt aattataagg atatataggt
      961 taacgtgtaa gttaacgtgt ccttaacgtg cttaacgtga cttaacgtgt tttaacgtgt
     1021 gtttaacgtg tgacttaacg tgtgttaaag gattatgata taaggttttc tttacttctt
     1081 aacgtgttta acgtgtatat ttttttattt tgcatgtaaa aataaaaaaa tcatttattt
     1141 aggatttttt tattataaat aaacatttta atagcttaca aattaggaat cccgcatgaa
     1201 tacttaaaat gacagaaaga agtttttccc ggccctgctc cccccttccc cacccataac
     1261 agcctgcagt aattgcatct atcggcacag ctctgaatat ccgctccatt gcaaaaatag
     1321 gagctgcctg agctgtgctt gcagcgtgca aagcatgata aagatctgct gcaggcggag
     1381 ccaggtccgg ctatgctata acttttattg caggagatat ccaggggaag cagcatgccg
     1441 ttat
//
LOCUS       DAOQWT010000451         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_113632_length_1439_cov_2.817197, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000451 DAOQWT010000000
VERSION     DAOQWT010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113632_length_1439_cov_2.817197"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKJ95_07775"
     CDS             <1..190
                     /locus_tag="PKJ95_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNR66172.1"
                     /translation="VVPEIETGNIFAKGLVYLAEAEPAGVLLGTTAPVIIVSRSDCPV
                     SKVRSIALAILMSVHQNK"
     gene            216..1289
                     /gene="buk"
                     /locus_tag="PKJ95_07780"
     CDS             216..1289
                     /gene="buk"
                     /locus_tag="PKJ95_07780"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNR66173.1"
                     /translation="MEKNYHILVINPGSTTTKLSLFKDDTMLFTETIAHSSDSLKMFN
                     SIIEQYEFREKVILEFLKSRSINLNTIDAVVGRGGLLEPLVSGTYRVNQRMINHLQQS
                     LFGEHASNLGAILAYQISRHNKISAYIVDPVVVDEMNEIARISGMPLLPRKSIFHALN
                     QKAVARKAAYDLGKNYNDVNLIVVHMGGGISVGVHQRGRVIDVNNALDGEGPFSPERS
                     GTVPVGDLVQLCFSGKYSQSEIKSMIKGKGGLIAYLNTNDVKMVIEKVMKGDQKSKLV
                     LEAMAYQISKQIGEGATVLKGNVDAIVLTGGISHSLQVVEMIKDRVSFISLVMVYPGE
                     EEMLSLCQGALRVLRGEEEERIY"
     gene            1235..>1439
                     /locus_tag="PKJ95_07785"
     CDS             1235..>1439
                     /locus_tag="PKJ95_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNR66174.1"
                     /translation="MSGCFKGFKRRRGGKNLLKDEIIIYTGSFGSGKTEIAINQSIEA
                     LKKKEKVVIVDLDIVNPYFRSRDM"
BASE COUNT          481 a          200 c          317 g          441 t
ORIGIN      
        1 agtagtgcca gaaattgaaa ctggtaatat atttgccaaa ggtctggtct atttggcaga
       61 agctgaaccg gcaggagtat tattaggcac aactgcgcct gttattattg tctctcgttc
      121 tgattgccct gtttctaaag tacgttccat tgcgctagct attttaatga gtgttcatca
      181 aaataaataa atataatata tataaggtga ttttcttgga aaaaaattat catatcctgg
      241 tgattaatcc gggttcaaca actacaaaat tgtcgttatt taaagatgat accatgttat
      301 ttactgaaac aatagcccat tccagcgatt cactgaagat gttcaatagt attattgaac
      361 aatatgaatt cagagaaaaa gtaattttag aatttttaaa aagtagatca attaatctta
      421 ataccattga tgctgtcgtg ggcagaggtg gattattaga gcctttggtt agtggaactt
      481 atcgggttaa ccaaagaatg ataaaccacc tccaacaaag tttatttgga gagcatgctt
      541 ccaatctcgg tgcaatatta gcttatcaaa tatccaggca taataaaatt tctgcatata
      601 ttgttgatcc ggtagttgta gatgaaatga acgagattgc acggatatct ggtatgcctc
      661 ttcttcccag aaagagcatc tttcatgcct taaatcaaaa agctgtagca cgaaaagcgg
      721 cttatgattt agggaaaaat tacaatgatg ttaatctcat agttgtacat atgggtggag
      781 gaatttctgt aggagtacat caaagaggga gggtgatcga tgttaataat gctttagatg
      841 gcgaagggcc tttttctcca gaaagaagtg gaacagtacc ggtgggggat ctggtgcaat
      901 tatgtttttc cggcaagtac tctcagagtg agattaagtc tatgataaaa ggaaaaggcg
      961 ggctgatcgc ctatttaaat actaatgatg taaaaatggt tatagaaaag gtaatgaaag
     1021 gagaccagaa atcaaaacta gttttggagg caatggccta ccagatttca aagcagattg
     1081 gagaaggagc tacagtactg aaaggtaatg ttgatgccat tgttctaact ggaggtatat
     1141 cacattcctt acaggttgtt gaaatgataa aagatagggt aagttttatt tccttagtta
     1201 tggtttatcc cggtgaagaa gaaatgcttt cactgtgtca gggtgcttta agggttttaa
     1261 gaggagaaga ggaggaaaga atctattgaa agatgaaata attatttaca caggcagttt
     1321 tggcagtgga aagactgaaa tagctatcaa tcagtcaata gaagctttaa agaaaaaaga
     1381 aaaggtagtt attgttgatt tagatattgt taatccctat tttcgttcta gagatatga
//
LOCUS       DAOQWT010000452         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_113636_length_1439_cov_2.723988, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000452 DAOQWT010000000
VERSION     DAOQWT010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113636_length_1439_cov_2.723988"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..921)
                     /locus_tag="PKJ95_07790"
     CDS             complement(145..921)
                     /locus_tag="PKJ95_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HNR66175.1"
                     /translation="MISIFEQVFKRVEQNERIALVTIVDVSGSSPGKLGSKMLVNKDG
                     LITGTIGGGMIEARVIEEAKLALKEDKGKFLSYQLNKEEASLEDWMICGGNIKVFIDV
                     IVPQDEMIIFGAGHIAVILSKFAKMVGFKVTIIDEREEFANRDRFPEADRIVVAEPDI
                     AIGEIKNFDSLYVVVVTRGHLKDEEALLSVINHGPKYIGMIGSKEKNKVIFQHLKEKG
                     ISEEEIAKIFAPIGVKIGAKTPEEIAVSIIAQIIGVKRGK"
     gene            complement(905..>1439)
                     /gene="yqeB"
                     /locus_tag="PKJ95_07795"
     CDS             complement(905..>1439)
                     /gene="yqeB"
                     /locus_tag="PKJ95_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HNR66176.1"
                     /translation="IMAKRNLGTSKEQAALVIALGPGFQAGLDVHAVIETKRGHYLGR
                     VIYQGNALPDTGVPGDIEGESEKRLLRAPEKGKIILEHQIGDLVHTDDVIATVNGVPL
                     KAQLSGVIRGLIYPDTQVSKGMKVGDIDHRGVREYCFTVSDKALSVGGGVLEAVCSYL
                     CKSGKGSGKKKKDDFNI"
BASE COUNT          406 a          350 c          190 g          493 t
ORIGIN      
        1 cctttttcat tttctaacca aaacttataa caaatcttca tttttaaaaa ccacctgaat
       61 tgtaataaaa aaatatttag aacttttaga atattataat taaaattttt ttcaggtctg
      121 attggaaaga ttgtattatt tgaactattt acctcttttc actcctatta tctgtgctat
      181 aatacttaca gcaatctctt caggagtttt tgctcctatc tttacaccta taggggcaaa
      241 tattttagca atctcctcct cactgattcc cttctctttc agatgttgaa agataacttt
      301 attcttttct ttactgccga tcataccaat atatttgggc ccatggttaa ttactgaaag
      361 tagtgcctcc tcatctttga gatgccctct ggttaccact accacataaa gggagtcaaa
      421 gtttttaatt tctccaatag ctatatcagg ttcagctacc acaattcgat ctgcttctgg
      481 aaatctgtct ctattggcaa actcttccct ttcatcaata atagtgacct taaagccgac
      541 catttttgca aatttggata ggataacagc aatatgtcct gctccgaaaa taatcatttc
      601 atcctggggg acaattacat caataaacac ctttatatta cctccacaaa tcatccagtc
      661 ttctaaagag gcttcttctt tattcaattg ataggataaa aatttccctt tatcttcttt
      721 taaagccaat tttgcctctt caataactct ggcttcaatc attcctcctc cgatagtacc
      781 ggttattaaa ccatctttat ttactaacat cttggaaccc agttttcccg gagaagaacc
      841 tgagacatca actatagtca ccagagcgat tctctcgttc tgctctactc ttttaaaaac
      901 ttgttcaaat attgaaatca tctttctttt ttttccctga accttttcct gatttacata
      961 aataactgca aacagcctct aatacccctc ctcctacgga aagagcctta tcagatactg
     1021 tgaaacaata ttctcttacc ccccgatggt caatatctcc caccttcatg cccttgctta
     1081 cctgggtatc aggatagata agcccacgta ttacaccgga caattgtgcc ttcagaggaa
     1141 caccattaac cgtagcaatt acatcatcgg tatgtaccag atcaccaatc tgatgttcca
     1201 agataatttt tcctttttca ggagctctca gcaaacgctt ttcagattca ccttctatat
     1261 ctcccggtac tccagtatcg ggcaaagcat taccctggta tatcaccctt cccaaatagt
     1321 ggcccctttt ggtttcaata actgcatgta catcaagacc cgcttgaaat cctggtccca
     1381 gtgctatgac taaagctgcc tgttctttgc tggttcccag attacgttta gccataata
//
LOCUS       DAOQWT010000453         1428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_115155_length_1428_cov_2.582666, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000453 DAOQWT010000000
VERSION     DAOQWT010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115155_length_1428_cov_2.582666"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PKJ95_07800"
     CDS             <1..607
                     /locus_tag="PKJ95_07800"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNR66177.1"
                     /translation="LPIFILLLFLFSFTLTAKADIYVIKDKKGNVIRVTNQHQMLKKE
                     KDAGYTITILIEDQKKAKKQVSQFKYNFKEKIDWIKVRAEAQLIEQTIRNQTEHELKY
                     PVYHVLATAYTPDEICCTPYADGYTATGYPAGYGSIAIDPDYGMFRYGDILYVEGYGL
                     GVADDCGSAIKGKHIDVCFNLGELKKADNWGRKNVRVWKIQ"
     gene            659..1393
                     /locus_tag="PKJ95_07805"
     CDS             659..1393
                     /locus_tag="PKJ95_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR66178.1"
                     /translation="MLKSVLVLLMLFGLANTSRAQSLFEKDCFATSEGELEITFIGHG
                     TLMLTFQDIVIHVDPWSNLADYSQLPKADIILITHEHGDHLDLQAIDILKREDTTIVL
                     TEACLDETAQGLAMKNGDNLIIEGVEISAVPAYNIEHKRDNGEPFHPKGRGNGYILNF
                     AELTVYIAGDTENIPEMSDLQNIDIAFLPMNLPYTMTPEMVADAAKIIRPKILYPYHF
                     GNTDTNQLIELLKEEKDIEIRIRKMS"
BASE COUNT          478 a          229 c          294 g          427 t
ORIGIN      
        1 tctccctatt tttattctgt tgttattctt atttagtttt accttaacgg caaaggctga
       61 tatctatgta attaaagaca agaaaggaaa tgtcattcgt gttaccaatc agcatcagat
      121 gctgaaaaaa gagaaagatg ccggctacac cattactatc ttaattgagg accagaaaaa
      181 agctaagaaa caggtatctc aatttaaata taattttaaa gaaaaaatcg attggataaa
      241 agttcgggct gaagcacagt taatagagca gaccatcaga aaccagaccg aacatgagct
      301 taaatatccg gtttaccatg ttttagccac tgcctatact cctgatgaga tatgttgtac
      361 accatatgcc gatggttata ctgctaccgg ttatcccgct ggttatggca gtatagctat
      421 agaccccgat tatggaatgt tccgttatgg tgatatattg tatgtggaag gctatggact
      481 gggagttgct gatgattgtg gcagtgccat taaagggaaa catattgatg tctgttttaa
      541 tttaggggaa ttgaaaaagg cagataactg gggcagaaaa aatgtcaggg tatggaaaat
      601 tcaatagttg ttaaactctt agtagtcaga tagtaataag attaagaagg agggtattat
      661 gttaaagagc gttttagtct tattaatgtt atttgggttg gcgaatactt ccagggcaca
      721 atctttattt gaaaaagatt gttttgcaac ctctgaaggt gaattggaga taacttttat
      781 cggacatggc acactcatgt taaccttcca ggacatagtt atccatgttg acccctggag
      841 taatttagcc gattatagcc aattacctaa agctgatatc attctgatta cccatgaaca
      901 tggtgatcat ctcgatttac aggcaattga tattcttaaa agagaagata ccactatagt
      961 attgactgaa gcctgtctgg atgagactgc acagggattg gcaatgaaaa atggagataa
     1021 tctgattatt gaaggtgtag agataagtgc agttccggcc tataatattg aacataagcg
     1081 cgataatggc gaacccttcc atcccaaagg gagaggcaat ggttatattc tcaattttgc
     1141 tgaattgacg gtctacattg caggagacac tgaaaatata ccggaaatgt cagatttgca
     1201 aaatatcgat atcgcttttc ttcccatgaa cttaccctat actatgactc cggagatggt
     1261 ggcagatgct gcaaaaataa taagaccaaa aatattatat ccctaccact ttggtaatac
     1321 tgataccaat caattaattg agttattgaa agaggagaag gatatcgaaa tacgaattag
     1381 aaaaatgtcc taatttttag taatagtaat acaatctttt acataata
//
LOCUS       DAOQWT010000454         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_116630_length_1417_cov_3.853157, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000454 DAOQWT010000000
VERSION     DAOQWT010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116630_length_1417_cov_3.853157"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PKJ95_07810"
     CDS             complement(<1..995)
                     /locus_tag="PKJ95_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR66179.1"
                     /translation="MKRGYDFDKVISRDANSGSYSIKWQGFEDRFPGYDVRGALGMWI
                     ADMDFLCPPEVITAVKKRAEHGIYGYLSPEAVEAFKRAAAGWFERRYNYQVPTDWMIF
                     SSSLDPSINAAIQEFTEPGDGVIIQNPVYYPFSNSIVNCGRTIRDNQLIEKNGFYTMD
                     FEELEMLAKKSDNKLFILCNPHNPVGRVWTEDELYQACKICSDNGVLIFSDEIHADFI
                     MKGHKFASAGKLSPEITENLLISFAPSKTFNIAGLTASLIVIPNRVIRERLEKRMRIN
                     NYPAPNVFAPIAGEAAYLYGDSYVDELTDYIGKNFDYVIEYFKENLPDIKMKKPEG"
BASE COUNT          432 a          264 c          233 g          488 t
ORIGIN      
        1 ccttccggtt ttttcatctt tatatccggt aaattttcct taaaatattc aatcacatag
       61 tcaaagttct tccctatata atcggttaat tcatctacat agctatctcc ataaagataa
      121 gccgcctcac cggcaatagg tgcaaataca ttgggagcag gataattatt aatgcgcatt
      181 ctcttttcca atctttctct tattactcta ttcggtatta caattaagga agcggtcagt
      241 ccggctatgt taaatgtttt actgggagca aaggagatga gcaggttctc agtaatttcc
      301 ggtgacaatt ttcctgctga tgcaaattta tgtcccttca ttataaaatc agcatgaatc
      361 tcatcagaga agatgagtac tccattatct gaacagattt tacaggcctg gtaaagctca
      421 tcttcagtcc agaccctgcc taccgggtta tgcgggttac agagtataaa gagcttatta
      481 tcagattttt ttgccagcat ttctaattct tcaaaatcca tagtgtaaaa accgtttttt
      541 tcaatcagtt gattgtctct tatagttctt ccacaattta caatactatt gctgaaaggg
      601 tagtaaacag gattttgaat aataacccca tctccgggtt cagtgaattc ctgaatagca
      661 gcattgatag agggatccaa actactgctg aagatcatcc agtcagttgg gacttgatag
      721 ttataacgtc tctcaaacca tccagcggca gctcttttaa aggcttctac tgcctcagga
      781 gaaagatagc cgtagatacc atgttctgct ctttttttaa ctgctgtgat aacctcagga
      841 gggcataaaa aatccatatc agctatccac atacccaagg ctccccggac atcataacca
      901 gggaatctgt cttcaaaacc ctgccatttt atactataag agccactatt ggcatctctg
      961 gagataactt tatcaaaatc ataacctctc ttcatatacc attatccttt cattttttaa
     1021 aatttgtagc attttttaat taaatcctat acagatatct tatacagata aatatataat
     1081 tacaattgtt attttatgta cagtatttaa gacggatact gttatgtata gaacttttta
     1141 ttctatcaac aggtctatta aaaaataata tactaggata aatttcatat gtcaaggcag
     1201 tatcgagtac atttctactt ggggcagcgt atagttttca agaacattat tatgtaaggc
     1261 aacagaggag ttgttgaacc ctagaatagt atcaaagtaa ctctttatat gggcatatgg
     1321 ttgataattt aatctaattt aatttagaaa tattttttaa tatgtatttg tagcttgtgt
     1381 tgaactttag gtgttttttt aggcctgtaa gtggtta
//
LOCUS       DAOQWT010000455         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_117702_length_1409_cov_3.343427, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000455 DAOQWT010000000
VERSION     DAOQWT010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117702_length_1409_cov_3.343427"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKJ95_07815"
     CDS             <1..386
                     /locus_tag="PKJ95_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008084894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HNR66180.1"
                     /translation="MVVEDGPTLTHGEMQFGAGVVAAQKNGAKEIIDPRPFALGSIKA
                     TYQRYPLIGKVLPAMGYSKEQIKELEATINNSDAQLVIIGTPIDLSKVMHIDKEYVRV
                     RYELQEIGCPDLKEILLEQFFKKHC"
     gene            429..>1409
                     /locus_tag="PKJ95_07820"
     CDS             429..>1409
                     /locus_tag="PKJ95_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNR66181.1"
                     /translation="MHEGQTIISFQYQPAKIVRGYTDKVLYLNLSSREIKIKEVPPKI
                     KEVLTGGRGYGLWYLWQATKPDTKWNDPENELIFCTGPLCGITQYSGCGKSHVVSLSP
                     ETGSANDNNAGGYFAPFLKFSGWDALEIQGKSEQDCIIFIDGNKGELTVQTYTNKDKN
                     SYLLVDELTEYFAEDEQDKSNISIVSAGQGAENSLLGILNFSWYDRKRGKVRYKQAAR
                     GGPGTVLRDKKIVAVIVKYKGVGSANNNPAQPELLKKAGQRITREILSLDHVQNGMRE
                     TGTINLLDHMNIHNCLPVHNFQYGSHQEATLIYNHIWKNRISQNQVGDSCW"
BASE COUNT          469 a          230 c          325 g          385 t
ORIGIN      
        1 taatggtagt ggaagatggt cctaccttaa ctcatggaga gatgcagttt ggcgccggag
       61 tggtagcggc acaaaaaaat ggtgcaaaag agataataga tcccagaccg tttgctctgg
      121 gcagtataaa agctacctat cagcgatatc ctctgattgg taaggtatta ccggctatgg
      181 gctacagtaa agaacagatt aaagagctgg aagctactat caataacagc gatgcccaat
      241 tggtgattat aggaactcct atcgatttaa gcaaagtaat gcatattgat aaggaatatg
      301 ttagagttag atatgaatta caggagatag gatgccctga tttgaaagaa attctgctag
      361 aacaattttt taaaaaacat tgctaaaatt gggatttatt agtagaaatt ttttaaagga
      421 ggttttcaat gcacgaaggt caaacaataa tatcatttca gtaccaacct gctaagatag
      481 tgaggggata tacagataaa gttctttatc tgaatctctc tagcagagaa ataaaaatta
      541 aagaggtgcc tccaaaaatt aaagaggttt taactggtgg gaggggttat ggactatggt
      601 atctatggca ggcaaccaaa ccggacacta agtggaatga tccggagaat gagttaatat
      661 tctgtaccgg gccactctgt ggaatcactc agtattccgg ttgtgggaag agtcatgtag
      721 tcagtctttc cccggaaact ggtagtgcca acgataataa tgcgggaggc tattttgcac
      781 catttttaaa attttcaggt tgggatgcat tggaaataca gggaaaatct gaacaggatt
      841 gtataatttt cattgacgga aacaagggag aattaactgt tcaaacctac accaataaag
      901 ataaaaattc atatttactg gtagacgagc taactgagta ttttgctgaa gatgaacagg
      961 ataaatccaa tatcagtatt gtttctgccg gtcagggagc tgaaaattca ttattaggta
     1021 ttttaaattt tagctggtat gaccgtaagc gtggcaaagt tcgctataaa caagcagctc
     1081 ggggagggcc aggaacagtg cttcgggata aaaaaatagt ggcagttatc gtgaaatata
     1141 agggtgtggg aagcgctaat aataatcctg ctcaacctga actgttaaag aaggccggtc
     1201 aacgtatcac ccgggagatc ctctccctgg accatgttca aaatggcatg cgggaaacag
     1261 gaactataaa tcttcttgac catatgaata ttcataattg tcttccagtc cacaattttc
     1321 aatatggcag tcaccaggaa gcaactttaa tctataacca tatttggaaa aatcgtattt
     1381 ctcagaatca ggtgggagat tcttgctgg
//
LOCUS       DAOQWT010000456         1405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_118260_length_1405_cov_2.998519, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000456 DAOQWT010000000
VERSION     DAOQWT010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118260_length_1405_cov_2.998519"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /gene="mgtE"
                     /locus_tag="PKJ95_07825"
     CDS             <1..760
                     /gene="mgtE"
                     /locus_tag="PKJ95_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNR66182.1"
                     /translation="MVQDVISVLDTENREVAARVIADYDLLAIPVINKQGKMVGIITV
                     DDIIDVLEEEVTEDIHKMVGSIEVYDDKLIKTSPFKKAKARLPWLLVCMAGEVISGSI
                     IELHSGILELTLALAFFIPVIMDMGGNIGTQSSTITVRGLATGQLRIDELWRNVWSET
                     KVGFLIGSTVGLLISIIAFFWQKSYVMGITIGLSLCFTVILAATMGTLMPLVFTYLKV
                     DPALASGPLITTIVDVVSLIVYFSIGTFLFTNLG"
     gene            828..>1405
                     /gene="eno"
                     /locus_tag="PKJ95_07830"
     CDS             828..>1405
                     /gene="eno"
                     /locus_tag="PKJ95_07830"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150542.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNR66183.1"
                     /translation="MSAIIDIYAREILDSRGNPTIEVETCLESGFVGIAQVPSGASTG
                     TYEAVELRDGDEKRYNGKGVLKAVHNVNEIIAPELVGLDSLDQCYIDQLMIDLDGTPD
                     KNKLGANAILGVSLSVAKAAAEYLCLPLYRYIGGINAKELPVPLMNILNGGKHADSGV
                     DLQEFMIVPVGAANFTENYRRAAETFQSLKKV"
BASE COUNT          402 a          239 c          324 g          440 t
ORIGIN      
        1 catggtacaa gatgtcatta gtgttttaga tactgaaaac cgtgaggtgg cagcaagagt
       61 gattgctgat tatgatttgc tggccatccc ggttattaat aagcagggca agatggttgg
      121 aatcattact gttgatgata tcattgatgt cttagaagag gaagtaactg aagatatcca
      181 caagatggta ggttctattg aggtttatga tgataaatta atcaagacca gccctttcaa
      241 aaaagcaaag gcaagattgc cctggctact ggtctgtatg gctggtgaag ttatttccgg
      301 ctctatcatc gaactccatt ccggtatttt ggagctaacc ctggcactgg cattttttat
      361 tcctgttatt atggatatgg gcggaaatat agggacccaa tcctctacta taacggtaag
      421 agggttagca actggacaac tacgcataga tgagttgtgg agaaatgtct ggtctgaaac
      481 caaagtgggc tttcttattg gctccacagt agggttgctt atctctatca ttgccttttt
      541 ctggcaaaag agttatgtta tgggtatcac cattgggcta tctttatgct tcacagtaat
      601 actggctgct actatgggaa cactaatgcc cctggttttt acttatttaa aggtagaccc
      661 ggccttagcc agcggccctt taattactac aattgttgat gtggttagcc tgatagtata
      721 ctttagtatc gggacttttc tttttacaaa tttaggatga acttcgcaaa atagtatctg
      781 tttaataaat taactttttt taatatttta tgtaagaggg taattttatg agcgctatta
      841 ttgatattta tgccagagaa attcttgatt ccagaggaaa tcctaccatt gaggtagaaa
      901 cttgtttgga aagcggattt gttgggatag ctcaggttcc ctcaggtgcc agtaccggca
      961 cctatgaggc tgttgagctg agagatggag atgaaaagag gtataacgga aaaggagtat
     1021 taaaagcagt acataatgtt aacgaaataa ttgcaccaga gttagtagga ttagattccc
     1081 tggaccagtg ctatattgat caactaatga ttgatttaga cgggactccc gataaaaaca
     1141 aattaggcgc taatgctatc ctgggcgttt ctctatctgt ggcaaaggca gccgctgaat
     1201 atctctgtct gcctctatat cgttatattg gtggtatcaa tgccaaagaa ttgccggtac
     1261 ctttgatgaa tatcttaaat ggaggcaaac atgccgatag tggtgtagat ttacaggaat
     1321 tcatgattgt gcctgtggga gcagccaatt tcactgaaaa ctatcgccgg gctgctgaaa
     1381 catttcaatc tttaaaaaag gtatt
//
LOCUS       DAOQWT010000457         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_118387_length_1404_cov_3.810230, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000457 DAOQWT010000000
VERSION     DAOQWT010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118387_length_1404_cov_3.810230"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PKJ95_07835"
     CDS             <1..304
                     /locus_tag="PKJ95_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66184.1"
                     /translation="HPTFREDNTLEKLWAENKESLELAGAIFQESVPDTFSGYPAIKA
                     LHTIKIGNNIHQYLRYSTVVDDMFYTFSFQAPAEEFEKYLEEVLTIVHSVLIKTVE"
     gene            complement(385..>1404)
                     /locus_tag="PKJ95_07840"
     CDS             complement(385..>1404)
                     /locus_tag="PKJ95_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HNR66185.1"
                     /translation="DYEEFRSNRPFTWVKTDVAACHPPANREVRGFPRGLDLMALLGS
                     QRARDILESTGDTRYSDYEKKFFELKDNIDTFREEDWLKNLYMNWLYVLKSLHSEVSE
                     GYPTFMQTTAWQDKQLNTTLASWAQLRHDTILYVKQSYTMAERGGMFRPPVVGYVEPV
                     PEFYSRLLNLTNITLAGFEKLVPPRILEDLRIESGLKRFAEVLERLLDITKKELANKL
                     LEDKDYDFIENFGAISASLIGTIAGGDINPDSLKSVMIADVHTEGNTEKVLEEGTGFV
                     KTAIIAYRLPQGYIVLGVGPVFSYYEFKQPMSERLTDEAWREILESGEAPEEPEWIGN
                     FSILK"
BASE COUNT          413 a          321 c          245 g          425 t
ORIGIN      
        1 ccatccgaca tttagagagg ataacacctt agaaaagctg tgggcagaaa ataaggaaag
       61 tttagagttg gccggggcaa tttttcagga atctgttccg gatacctttt caggataccc
      121 ggcaattaaa gccctgcata ccatcaagat aggcaataat attcaccaat atcttcgcta
      181 tagcactgtt gtagatgata tgttttatac ctttagcttc caggctcctg ccgaagaatt
      241 tgaaaaatac ttagaagaag tacttactat agttcattct gtattgataa agactgttga
      301 ataggaaaaa ttgggtcggg tcattgaggc ctgaccccaa caggtaagta aactggtaaa
      361 tctttctaat ttttgaatta ctcattattt cagtattgag aaattcccaa tccactcagg
      421 ctcttccggt gcttcgccgc tttctaatat ttctctccag gcttcatcgg ttaaccgttc
      481 actcattggc tgtttgaatt cataatagct aaaaactggc cccactccta acacaatata
      541 tccctgggga agcctatagg caataatggc agtcttaaca aaaccagtgc cctcttccag
      601 tactttttca gtatttcctt cagtatgaac atcggcaatc atcactgatt ttagagaatc
      661 cgggttaata tctcctccgg caattgtacc aatcaaactg gcggagatag caccaaaatt
      721 ttcaataaaa tcataatctt tatcttctaa taacttatta gccaattctt ttttggtaat
      781 atccagcaac ctctccagca cctcggcaaa tcgtttcaac cctgactcta ttcttaaatc
      841 ttccagtatc cgtggaggaa ccaatttttc aaatccagcc agagtaatat tggttaagtt
      901 taatagtctg ctgtaaaatt caggaaccgg ctctacatat cctactaccg gaggtcggaa
      961 catgccgcct ctctcagcca tggtataact ctgtttcaca tataaaatag tatcatgccg
     1021 taattgagcc caggaagcca gagtagtatt taattgctta tcctgccagg cagtggtctg
     1081 cataaaggta ggataacctt cactgacttc actatgtaaa gacttcaaaa cataaagcca
     1141 attcatataa agatttttta accagtcttc ctctctaaaa gtatcaatat tatcttttaa
     1201 ttcaaagaat tttttttcat aatcactata ccgggtatct ccggtagatt ccagtatatc
     1261 ccgggctctc tgtgaaccca gcagagccat taagtccaat ccccggggaa atccccggac
     1321 ttccctgtta gccggaggat gacaggccgc aacgtctgtt ttaacccagg taaatggcct
     1381 gttgcttctg aattcctcat aatc
//
LOCUS       DAOQWT010000458         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_118538_length_1403_cov_4.588279, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000458 DAOQWT010000000
VERSION     DAOQWT010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118538_length_1403_cov_4.588279"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKJ95_07845"
     CDS             complement(<1..306)
                     /locus_tag="PKJ95_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR66186.1"
                     /translation="MAKIYYDKDADKSYLEGKCVAVIGYGSQGKGQALNLKDSGVNVI
                     VGLKENGASWAKALNDGFKVETIEKAAKQADIVQILIPDEVQPQTCYTNKVLENLEMG
                     "
     gene            complement(348..842)
                     /gene="ilvN"
                     /locus_tag="PKJ95_07850"
     CDS             complement(348..842)
                     /gene="ilvN"
                     /locus_tag="PKJ95_07850"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNR66187.1"
                     /translation="MKHTIAILVKDHPGVLARISSLFNRRRFNIESIAAGHSEKEGVT
                     RITIVTEGDDAILEQITKQLNKLVDVLRVKDLPREHSVERELALIKVSTKSVDERSEI
                     MQLVDTFRGHIVDIDKKSLTIEVTGAQEKINAKLKLLESFGILEIARTGKISLSRGAQ
                     RILN"
     gene            complement(839..>1403)
                     /locus_tag="PKJ95_07855"
     CDS             complement(839..>1403)
                     /locus_tag="PKJ95_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNR66188.1"
                     /translation="EQTKDDAIVVTDVGQHQMWAAQYYKFSHPRSFITSGGLGTMGFG
                     LPAAIGAQVGCPEKRVFCIAGDGGFQMNIQELATAIYNKISVVVLIINNGYLGMVRQW
                     QELFFEKRYSQTSLEGSPDFAKVAQAYGGKGVRVANRDDFDIALTQALSEKTFFLIDC
                     LIPSEENVFPMVAPGEPINKMLGVNRV"
BASE COUNT          390 a          329 c          212 g          472 t
ORIGIN      
        1 tcccatttcc agattttcca atactttgtt ggtatagcag gtttggggct gtacctcatc
       61 cggtattaga atctggacta tatctgcttg tttagcggct ttttctattg tctcaacctt
      121 aaaaccatca tttaatgcct tagcccatga tgcaccattt tcctttaaac ctactataac
      181 gtttacacca ctgtccttga gatttaaagc ctgacctttt ccctggcttc cataccctat
      241 aacagcaaca cattttcctt ctaagtaact tttatcagcg tccttatcgt aataaatttt
      301 tgccatctaa tttttcctcc ttaaaatata aattttttat ttttttgtta atttaatatt
      361 ctttgtgccc ctctggaaag agagatttta ccggtacgag caatttctaa aattccaaaa
      421 gattccaata gttttaattt ggcatttatt ttttcctgtg ccccggttac ctcaattgtc
      481 aatgactttt tatcaatatc gactatatgt cctcgaaaag tgtctaccag ttgcataatt
      541 tcactcctct catcgacaga tttagtactc actttaatca aggccaactc tctttccaca
      601 gaatgttccc tgggaagatc ttttaccctc aaaacatcaa ccaatttgtt aagctgtttg
      661 gtaatctgtt ccagaatagc gtcatctcct tctgttacga tagtaattct ggtgacacct
      721 tccttttctg aatgtcctgc agcaatactt tcaatattaa atcttctgcg attaaagaga
      781 ctggaaatac gggccaatac acctggatgg tcttttacta aaatagcaat agtatgtttc
      841 atactctatt cactcctaac attttattga taggctctcc tggtgccacc ataggaaaga
      901 cattttcttc agatggaatc agacaatcta ttaagaaaaa ggtcttttca gataatgcct
      961 gagtaagggc aatatcaaaa tcgtctctat tggcaaccct gactcccttt cctccgtaag
     1021 cctgagccac tttggcaaaa tccgggctgc cttcaaggct ggtttgtgaa tatctttttt
     1081 caaagaatag ctcctgccat tgacgcacca taccaagata gccattattt ataatcaaaa
     1141 ctacaacaga gattttatta tagatggcag tagccaattc ctgaatattc atctgaaaac
     1201 caccatctcc tgctatacaa aaaacccttt tctcaggaca tcctacctga gcgccgatag
     1261 ctgccggtaa gccaaagccc attgttccca gtcctcctga agtaataaaa ctacggggat
     1321 ggctaaactt ataatattgg gctgcccaca tctgatgttg accaacatcg gtgaccacaa
     1381 tagcatcatc cttggtttgt tca
//
LOCUS       DAOQWT010000459         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_119017_length_1400_cov_2.603717, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000459 DAOQWT010000000
VERSION     DAOQWT010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119017_length_1400_cov_2.603717"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PKJ95_07860"
     CDS             complement(<1..1013)
                     /locus_tag="PKJ95_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNR66189.1"
                     /translation="MKTGQFYVERDDILLKRIFKIFILLIIPILLLPAPFSLHLEAES
                     ATVYIIPVKGQIEPGWLLFLKRSLKEARESGAQAVILEVDTPGGFIDTAQEAKKLLDE
                     LTIPVYGLVNNDALSAGAYLTLATDGFYMTPGSTIGAAEPILLTGKEVDEKILSFWEA
                     EMRSTAEKQGKDPLIAAAMVRRPLAIEGLVREGELLTLTTVQAEKISFSDGTVNSVEE
                     LLDKVNLSGSNIIYSSATFWERLSGWLINPIIATILLMMGFFFMVVEIMTPGFGVGGL
                     LSLIAFGLYFGGHFLTGVSGWPAIFLFGLGIILLLIEAFIPGFGIFGISGLIAVIIAI
                     VLAA"
     gene            complement(1104..>1400)
                     /locus_tag="PKJ95_07865"
     CDS             complement(1104..>1400)
                     /locus_tag="PKJ95_07865"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNR66190.1"
                     /translation="MWPQTTPPSGLAFWNNYLFIATLRSEALIRIEVGQRDNTYEIRN
                     IDRLFAYDWESGAYGRLRDAVVGPDNALYVLTNNYDGRGWPYEGDDKILRLTLK"
BASE COUNT          428 a          332 c          238 g          402 t
ORIGIN      
        1 gctgcaagaa caattgctat aataacagct attaatccac tgataccaaa tataccaaaa
       61 cctggtataa atgcctctat caataatagt atgataccaa ggccaaagag aaagatggct
      121 ggccatccac tgacaccggt caaaaaatgt cctccaaaat agagaccaaa agcgattaaa
      181 ctgagcaatc cgcctacccc aaaaccggga gtcattatct caactaccat gaagaagaac
      241 cccatcatta gaagtatagt tgctataata ggatttatta accatccgct caatctttcc
      301 caaaaagtag ctgaactata aattatatta ctgcccgata aattgacctt gtccagcaat
      361 tcttctacag aattaaccgt gccatcactg aaggatattt tttcagcctg cacggtagtc
      421 agggttaata actccccctc tcttaccagt ccttcaatag ccagtggtct acgcaccatg
      481 gcggctgcta taaggggatc tttcccctgt ttttccgcag tacttctcat ctctgcttcc
      541 cagaaggaga gtatcttctc atccacttct ttccccgtta gaagaatcgg ttcagctgct
      601 ccgatggtag aaccgggtgt catataaaac ccatcagtag ccagtgtcag ataagctcca
      661 gcagaaagag catcgttatt gaccaggcca tatactggta tagtaagctc atcaagtaat
      721 ttttttgctt cttgagctgt atcgataaaa cctcccgggg tatccacctc tagaataact
      781 gcctgagccc cggactccct tgcttctttc agagagcgtt tcaaaaagag cagccagcct
      841 ggttcaatct gccccttaac cggtattata tagaccgtag ctgattctgc ttccaggtgt
      901 aatgaaaacg gagcaggcaa caagagtatt ggtattatta gcagaataaa tattttaaat
      961 attctcttta gcaagatatc atccctttca acatagaatt gacctgtttt caataataat
     1021 tcattgaaat aggaactttt ttagaatcat catcattttc ctatggttgg tatcatggta
     1081 tctttaataa ttataacata attctatttt aaagttagac gcaaaatctt atcatcgcct
     1141 tcgtagggcc atcctctccc atcataatta ttggtcagca catataaggc attgtcaggt
     1201 cctaccactg catcacgtaa acgtccatag gcaccacttt cccaatcata ggcaaacaat
     1261 ctgtcaatat ttcttatttc ataggtatta tctctttgcc caacctcaat ccttatcaga
     1321 gcttcactgc gtagtgtagc aataaataaa tagttattcc aaaaagccaa accggaaggg
     1381 ggagtggtct gtggccacat
//
LOCUS       DAOQWT010000460         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_120280_length_1391_cov_2.895210, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000460 DAOQWT010000000
VERSION     DAOQWT010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120280_length_1391_cov_2.895210"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..616
                     /gene="hypA"
                     /locus_tag="PKJ95_07870"
     CDS             209..616
                     /gene="hypA"
                     /locus_tag="PKJ95_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypA"
                     /protein_id="HNR66191.1"
                     /translation="MHEWSLAEAVLKSSIKEAKKRNLKKLLEVKIVLGELQAIEEEIV
                     KFSLDSLKKGTMAEEATFVFINEKATFKCRNCQNEWTLKEANINEESIKESIHFVPEV
                     VHSFIRCTRCGSPDFEVVEGRGIYIDEITGEDL"
     gene            669..>1391
                     /locus_tag="PKJ95_07875"
     CDS             669..>1391
                     /locus_tag="PKJ95_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNR66192.1"
                     /translation="MFDPREVVSREKFAIMKCIIPVVSGKGGVGKSVVSTALSLALAE
                     RHKTGLLDLDFWGASAHILLNASDQFPEEEKGILPEEIRGVKFMSVSYYTQDKPLPIR
                     GKEYSDAFLELMAVTRWNDIECLVIDMPPGMADPFLDLLQYVKNGSFLVITTSSRLSS
                     NIVSKTLKILKEQNLKVLGLIENMRSVGDDSERIRDLANSFNVKHLGYIPYYHNLDIH
                     LGNLQDFISTDFFQKIKAISMGL"
BASE COUNT          490 a          168 c          293 g          440 t
ORIGIN      
        1 tgatttttat tgatagatat gttttgtttt agaagagttt tatgaaagaa ttaaaaaaaa
       61 taatgttata tgctaaaatg aaatgtattg cgttaaattg aaatttataa aaaaatgaaa
      121 aattatagac cagaatatta aaatagtatt ttgggagagg gatcattaaa tcatttgttg
      181 acctaaataa atttaaggag gcagtaaaat gcatgagtgg tctttagctg aagctgtatt
      241 aaaatcttct attaaagagg caaaaaaaag aaatttaaaa aaattattag aagttaaaat
      301 tgttttggga gaactgcagg caattgaaga agagattgta aaattcagct tggatagttt
      361 aaaaaaagga actatggcag aagaggcaac gtttgttttt ataaacgaaa aagctacttt
      421 taaatgtcga aattgccaga atgaatggac tttaaaagaa gcaaatataa acgaagaatc
      481 tattaaagaa tctattcact ttgttccgga agttgttcat tcttttataa gatgtaccag
      541 gtgtggtagc cctgattttg aggtagtcga aggaaggggt atttatattg atgagattac
      601 cggtgaggat ttataaaaaa atttaaattt agaaattcct ataaaaatat tttcagcagg
      661 aggatatcat gtttgatccg agagaagttg tatcaagaga aaaatttgcc attatgaaat
      721 gtatcatccc ggttgtaagc ggtaaaggag gagtggggaa atccgtagtg tctacagcat
      781 tgagtctggc tttagcagag agacataaga ctggcttgct tgatttggat ttctggggtg
      841 cctccgccca tattttattg aatgccagcg atcagtttcc tgaggaagaa aaaggaatat
      901 taccggaaga gataagaggg gtaaaattta tgtctgtctc ttattatact caggataagc
      961 ccttacccat aaggggaaaa gaatatagcg atgcgttttt agagctcatg gctgtaacca
     1021 ggtggaatga cattgagtgt ttagttatag atatgccgcc aggaatggct gaccccttcc
     1081 tggatttact tcaatatgta aaaaatggaa gttttttggt tattaccacc tcatccagac
     1141 tttctagcaa tattgtctct aaaaccctta aaatattaaa agaacagaat ttaaaagttc
     1201 ttggcttgat tgaaaatatg cgctcagtcg gagatgatag tgaaaggata agagacctgg
     1261 ccaatagctt taatgtgaaa catctgggtt atattcctta ttatcataat ttagatattc
     1321 atctgggtaa cctgcaggat tttatttcca ctgatttttt tcaaaagatt aaagcaatta
     1381 gtatggggct g
//
LOCUS       DAOQWT010000461         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_120319_length_1391_cov_2.407934, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000461 DAOQWT010000000
VERSION     DAOQWT010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120319_length_1391_cov_2.407934"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PKJ95_07880"
     CDS             complement(<1..390)
                     /locus_tag="PKJ95_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNR66193.1"
                     /translation="MEIKQNIAVIGVGKMGTTLIDSLLKNQVINPTQIYGTTIQEVHA
                     NRVQEKYQINTGTDNLEAASKSDIIILAVKPQMISQVLKQIADALSKKKFVISIAAAI
                     STTFIEKQLNDKNISVIRAMPNIASLVN"
     gene            complement(541..>1391)
                     /locus_tag="PKJ95_07885"
     CDS             complement(541..>1391)
                     /locus_tag="PKJ95_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNR66194.1"
                     /translation="NIQEIRKIGYIFEIVLKHAPSLYAQKIVVATGGRAAPQLGATGD
                     GYHWALKLGHHLERQFPALVQLTTNISTLRLLNKLKLTEVVANLFIDDKLITSVKEDL
                     LFLSNGISGTSVFTISRLASEALSRGKKVAVRINFTPDLNREELDNIFFSKKKSESFK
                     PLSLLLQGILPEKLSQFILKECNIRFDSAISQLADSEIETIIANILDFYLPINGTQSW
                     KYAQITCGGISVREVEPKTMESKLVPDLYFAGEILDVDGDCGGYNLQWAWSSGYLAGK
                     SAVGNS"
BASE COUNT          398 a          274 c          244 g          475 t
ORIGIN      
        1 gttaaccaat gaggcaatat tgggcatagc ccgtataaca gaaatatttt tgtcattgag
       61 ttgtttttca ataaaagtgg tactgattgc ggctgcaatg gagataacaa attttttctt
      121 acttaaggca tcggcaatct gttttaaaac ctgggaaatc atctgtggct ttactgccaa
      181 tataataata tcacttttag acgctgcctc caggttatct gttccggtat tgatctggta
      241 tttttcttga acacgattgg catgaacttc ctgtattgtg gtgccataaa tttgagtggg
      301 gtttataacc tgatttttta gcaaactatc tattaaagtg gtgcccattt tcccaacacc
      361 gataacggca atgttctgtt ttatttccat ttaaatatac ctttctactc tgtttttacc
      421 tctttacata gaagaccata aaagaatatt ggttgttata ttaaagcaaa ttctcccttc
      481 ctgtcaagaa agaaagggag aattttataa attttttttc tagttttatt tcgctttcgc
      541 tcaactatta ccgactgctg attttcctgc taaatatcct gaactccagg cccattgcag
      601 attatagcca ccacaatcac cgtcaacatc gagtatctct ccggcaaaat agagatcggg
      661 taccaattta gattccattg ttttcggttc aacttcccta acggatatcc cgccacaggt
      721 aatctgagca tatttccagg actgagtacc atttataggt aggtagaaat ctaaaatatt
      781 agcaattatc gtttcaatct cactatctgc taattggcta attgctgaat caaatcttat
      841 attgcattct tttaatatga attggcttag cttttccggt aaaatacctt gcaacaacag
      901 gcttaaaggt ttaaatgact cacttttctt tttgctgaag aaaatattgt ccagttcttc
      961 tctatttaag tcaggtgtaa aattaatccg gactgctact tttttgcccc gggaaagggc
     1021 ttcactggcc agtctgctga tagtgaatac tgaagtaccc gaaataccat ttgataaaaa
     1081 gagcaaatcc tcttttacac ttgttattaa cttatcatct ataaaaaggt tagcaactac
     1141 ttcagtcaat tttaacttat tcagcagacg gagagtggaa atgttagtag tcaattgtac
     1201 cagggcgggg aattgccgct ccagatgatg acccagtttt aaagcccagt gatagccatc
     1261 acctgttgct cccaattggg gggcagcccg accaccggtt gctactacta ttttttgtgc
     1321 gtataaagag ggtgcatgct tcaaaactat ttcaaagata tagccaattt ttcttatttc
     1381 ctgaatattt a
//
LOCUS       DAOQWT010000462         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_120377_length_1390_cov_8.907865, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000462 DAOQWT010000000
VERSION     DAOQWT010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120377_length_1390_cov_8.907865"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..584)
                     /locus_tag="PKJ95_07890"
     CDS             complement(75..584)
                     /locus_tag="PKJ95_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66195.1"
                     /translation="MKKKNQFRKRRIIVTIVIITIVTIVYYQIDYKRHFISNNNNTEF
                     ITIWQRIGNDCYIVPGKYYWIFAPKDNFIKTVNYRNYIGIVWNTEDKYSYKISIYNKF
                     KVIDLETDICIYSSNDSLLLEYQILESLDIQRGKRIKNPKADSLKRVYDYDYVDLNRI
                     YGIKVHDYK"
     gene            complement(581..922)
                     /locus_tag="PKJ95_07895"
     CDS             complement(581..922)
                     /locus_tag="PKJ95_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66196.1"
                     /translation="MLLTAKKYDFKRNGDPENNDRENHHRGSEFYRKEDGTKVYASAR
                     DAGNFSAGYYAASQGLFWKQARFGFDGLEKIKSALKSHFVSGEGRQSTHAQALGYARR
                     KYGMDVGPSGK"
     gene            complement(897..1073)
                     /locus_tag="PKJ95_07900"
     CDS             complement(897..1073)
                     /locus_tag="PKJ95_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66197.1"
                     /translation="MFSFYNEDKQDREDQVGWEGTFDVNSTESGDLVKKFMGEADGIE
                     LGEYMENATDGKKV"
     gene            complement(1083..1343)
                     /locus_tag="PKJ95_07905"
                     /pseudo
     CDS             complement(1083..1343)
                     /locus_tag="PKJ95_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          411 a          316 c          154 g          509 t
ORIGIN      
        1 ccggctccag accggttggt tacccaagca gctacaaatt catttacaca aaattattta
       61 cacgcccatg ctttctattt ataatcatgc actttaattc cataaatcct atttaaatca
      121 acataatcat aatcatatac tctctttaga ctatctgcct tcggattttt tattcttttt
      181 cctctctgta tatctaaaga ttctaaaatt tggtactcca acagcaatga atcatttgag
      241 gaataaatac aaatatctgt ttctaaatca attactttaa acttattata aatggatatt
      301 ttataagaat acttatcttc tgtattccag actatcccaa tataattcct gtaatttact
      361 gtttttataa agttatcttt gggagcaaat atccaataat atttacctgg cactatatag
      421 caatcattac ctatacgctg ccaaatagta ataaactcag tattattatt gttgctaata
      481 aaatgccttt tataatctat ttgataataa actattgtaa ctatcgtaat tattacaata
      541 gttacaataa tccttctttt tctaaactga ttcttctttt tcattttcct gagggcccta
      601 catccatacc atattttcgg cgtgcatagc ctaatgcttg cgcatgcgtg ctttgtctac
      661 cttctccaga cacaaaatga ctttttagag cagacttgat tttttccaaa ccatcaaatc
      721 caaatcgagc ttgcttccaa aataatcctt gagatgctgc ataatatcca gcagaaaaat
      781 tccctgcatc tctagctgaa gcatatactt tggtgccatc ttcttttctg taaaactctg
      841 accctctatg atgattctct ctatcattat tctcagggtc tccatttctc ttgaaatcat
      901 acttttttgc cgtcagtagc attctccata tattcaccta attctatacc atcagcctct
      961 cccataaact tcttaaccag gtctcctgat tccgtagaat taacatcaaa agtcccttcc
     1021 catccaactt ggtcttctct atcttgttta tcttcattat aaaatgaaaa cattgttaaa
     1081 gattacccta tactctttcc tggtttggaa aagcttccat ctttgttttg agtaacaata
     1141 tatatccctc tatctttatc ttctaaatta ccaccaacaa ctttataagt tccatctcca
     1201 ttatcagtaa cccaagttga cattccatca gggtctatga attttattgg attattcatc
     1261 acatatgcat aagggctatg actataatat tcttccgcca atgggtcaac cgttgtccac
     1321 ctccctattt gctggtcata gaaccttgcg ccgtaatcct gttagtctac tcccaaaaaa
     1381 ctacacttca
//
LOCUS       DAOQWT010000463         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_120383_length_1390_cov_5.522846, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000463 DAOQWT010000000
VERSION     DAOQWT010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120383_length_1390_cov_5.522846"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>1390
                     /locus_tag="PKJ95_07910"
     CDS             17..>1390
                     /locus_tag="PKJ95_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR66198.1"
                     /translation="MLSDKFGLEGIDSSCRNANIKDNDRDSTFTMPDIYLSPSFGTIE
                     PNSSVTCILTFIAGNTPGTYNYELQLSSNDPVSPLITVPLKYVVNSPIAYIPDDNFRW
                     AINDALGQPSNYHPTIADLQGLTGTLDAESRNIISIEGAQYLTNLQELYLNSNQISDL
                     SPLSGLTNLQKLRLSGNQISNLSPLAGLTNLQRLNLDSNQISNISPLAGLTNLWSLTL
                     SCNQISDLSPLAGLTNLRWLYLSSSQISNLSPLAGLTNLRELYLYNNQISDISPLEEL
                     TNLQVLWLNSNQISDLSPLAGLTNLRNLWLDYNQISNIRPLAGLNNLQYLGLDSNQIS
                     DLSPLAGLTNLRNLLLFSNQISDLSPLAGLTNLEYLELYINQISNISPLAGLTNLQVL
                     WLNYNQISDISPLSGLINLWALNLDDNPISYESMLLAQSWSLPWSTTSYNPLSPCYPD
                     PNRGATNV"
BASE COUNT          392 a          290 c          264 g          444 t
ORIGIN      
        1 tgtaagtaga gatagaatgt tgtctgataa atttggttta gagggaatag atagtagttg
       61 cagaaatgca aatataaaag ataatgatag agatagcaca ttcacaatgc cggatattta
      121 cctttcgcct tctttcggaa ctatagaacc caattcttct gtaacctgca tcctgacttt
      181 cattgctgga aatacacctg gcacctataa ctatgaactt cagctctcat ccaatgatcc
      241 tgtctcacct cttatcacag ttcctcttaa atatgtagtt aacagcccta ttgcctatat
      301 tcctgatgac aattttagat gggcaatcaa tgatgcttta ggtcaaccct ctaattatca
      361 ccctacaata gctgacttac aaggtctaac tggaaccctt gatgctgaga gtcggaatat
      421 tattagcata gaaggtgctc aatatctgac taatctgcaa gagctgtatc ttaattctaa
      481 ccagataagt gaccttagtc cgttatcagg attaactaat ctacaaaagc tgcgtcttag
      541 tggtaaccag ataagtaacc ttagtccttt agccgggctg actaatcttc aaaggctgaa
      601 tcttgattct aatcagataa gtaacatcag tcctttagcg gggctgacta atctttggtc
      661 tttgactctt agctgtaacc agataagtga ccttagtcct ttagccgggc tgactaatct
      721 gcgatggctg tatctttctt ctagccagat aagtaacctt agtcctttag ccgggctgac
      781 taatctgcga gagctgtatc tttataataa ccagataagt gacattagtc ccttagagga
      841 gctgactaat ctgcaagtgc tgtggcttaa ttctaaccag ataagtgacc ttagtccttt
      901 agccggtctg actaatctgc gaaatctgtg gcttgattat aaccagataa gtaacattag
      961 acccttagcg ggtctgaata atctgcaata tctgggtctt gattctaacc agataagtga
     1021 ccttagtcct ttagccgggc tgactaatct gcgaaatctg cttctttttt ctaatcagat
     1081 aagtgacctt agtcctttag ctgggctgac taatctggaa tatctggaac tttatataaa
     1141 ccagataagt aacatcagtc ctttagccgg gctgactaat ctgcaagtgc tgtggcttaa
     1201 ttataaccag ataagtgata ttagtccttt atccgggctg attaatcttt gggctcttaa
     1261 tcttgatgat aatcccatat cttatgagtc aatgctgctt gctcagagtt ggtctttacc
     1321 ttggtcaaca acaagttaca atcccctttc accttgttat cctgatccga atagaggtgc
     1381 tacgaatgtt
//
LOCUS       DAOQWT010000464         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_120613_length_1389_cov_2.412294, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000464 DAOQWT010000000
VERSION     DAOQWT010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120613_length_1389_cov_2.412294"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PKJ95_07915"
     CDS             complement(<1..524)
                     /locus_tag="PKJ95_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66199.1"
                     /translation="MESNSKVIGILGTAKNTGKTTTFNFLLEWSNSKKIKSGLTSIGY
                     DGEDIDNITGLPKPRIFIEKGNTIATAEQCLFPLKDRIEIIEKTSVNTALGRVIIGKA
                     KKEGMVILAGPNKGIDLQTIIQKLKQQRVELILVDGALNRMIPLIHCDALIIATGASR
                     HTDIGLLVDEIESMT"
     gene            complement(536..>1389)
                     /locus_tag="PKJ95_07920"
     CDS             complement(536..>1389)
                     /locus_tag="PKJ95_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNR66200.1"
                     /translation="AKIKLSLHKETLWTVRAHRGLNTPETTVIAGRLGADLTKIHIVY
                     GSLNGGTDPARLTIDGLKAIQFAAKYNMPFDEPTNEELGGVPAYKSFAGLLIIADLAL
                     KLKAQPILKPLFCYSPDVMISGKMDDNYIDYNVAKIKSLRSIIDAPIWPGEPIGFMTH
                     SEDRVQSTLSTALHSALAISLQVDAITIASSDEAYSRGPISLASRIDTLRGAQEVFRF
                     LGKAKIKPTGKAKEYEKEITEGIEKTLEKVAKRGSFVDALYEGLLGSPQDGAYPGRAG
                     RDTVFEG"
BASE COUNT          404 a          283 c          220 g          482 t
ORIGIN      
        1 gtcatacttt caatttcatc caccagtaaa ccaatatcag tatgacgaga ggcacctgtt
       61 gctattatta atgcatcaca atgaatcaat ggtatcatcc tattcaaagc cccatcaacc
      121 agtataagtt ccactctttg ttgttttaac ttttgaataa tagtctgaag atctatacct
      181 ttattcggac ctgccaatat caccattcct tccttttttg cttttccaat tattacacgg
      241 cctaaagcag tattaacaga tgttttttca attatttcta ttctatcttt taaaggaaac
      301 aggcattgtt cagctgtggc aatggtatta cccttttcaa taaaaatgcg gggtttgggt
      361 aatccggtga tattatcaat atcttctccg tcataaccga tactggttaa acctgatttt
      421 attttttttg aatttgacca ttctaataaa aaattaaaag ttgtagtctt ccctgtattt
      481 ttggcagttc ccagtattcc aattacttta ctattactct ccataaaata tttctttatc
      541 cttcaaagac agtatctctt ccagcacggc cagggtaagc tccatcctga ggacttccta
      601 ataatccttc ataaagagca tcaacaaaac tgcctctttt ggcaactttt tctaaagttt
      661 tttctattcc ttctgttatt tccttctcat attcctttgc tttgccagtc ggttttattt
      721 ttgcctttcc taaaaaacga aatacttcct gtgctcctct taaggtgtca attctggagg
      781 caagtgaaat tggccctctg gaataagctt catctgaaga tgctatggtt atagcatcta
      841 cctgaagaga aattgcaaga gcagagtgta aagcagtgga taaagtagat tggactctat
      901 cttcagaatg agtcataaaa ccaataggtt caccaggcca gatgggagca tcaattatgg
      961 agcgcaggct tttaattttg gccacattat agtcaatata attatcatcc attttcccgc
     1021 taatcataac atcgggagaa taacaaaaaa ggggctttaa tataggttga gcttttaatt
     1081 ttaaagctaa atcggcaata atcaacaaac cagcaaatga tttataggct ggaacacccc
     1141 ctaactcctc atttgttggc tcatcaaaag gcatattata ttttgcagca aattgtattg
     1201 ctttcaagcc atcaatagtt aatcttgccg ggtcagttcc accattgagg gatccatata
     1261 ctatatgtat tttagttaaa tcagctccta atctacctgc aattacagtt gtctcagggg
     1321 tatttaatcc acgatgggct ctgactgtcc ataatgtctc tttatgaagt gaaagtttta
     1381 tttttgcca
//
LOCUS       DAOQWT010000465         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_122859_length_1373_cov_3.038695, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000465 DAOQWT010000000
VERSION     DAOQWT010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122859_length_1373_cov_3.038695"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PKJ95_07925"
     CDS             complement(<1..812)
                     /locus_tag="PKJ95_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HNR66201.1"
                     /translation="MLLKDRPLTCLLLIVIIFLSLSVQSCSSNSTNINDNDNTTPILS
                     CEIVNIFPHDPEAFTQGLIWDDGYLYESTGLYGSSSLRQVELKTGKAIKNIPLADNYF
                     AEGITIFDDKIFLLTWREEMGFIYTRDTFQLLDTFSYPYEGWGITTDGDFLIISDGTP
                     VLHFLDPKTLDEIKQVKVHDGLFLVNWINELEYIEGYIYANIWQSDRIAVIEPGTGKI
                     VFWLDLYGILDNVQHHENVDVLNGIAYDAENNRLFVTGKLWPAIFEIKMIET"
     gene            complement(818..>1373)
                     /locus_tag="PKJ95_07930"
     CDS             complement(818..>1373)
                     /locus_tag="PKJ95_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR66202.1"
                     /translation="FNKKYQLAGHLFQNRFKSENVENDAYLISLIRYIHQNPVKAGIA
                     LSPEEYPWSSCFYYFFPGKNIYKLLDIEDILKIFSYDIEEAKEQFKGFSQKEETTHKF
                     IEYKDKNEQPKQINTEKKFTNFVNKFVDEHKIFLQDLFKRENKSIRDQLILKLKDESI
                     FSNKEIAEFLGITRGIIQNIRREK"
BASE COUNT          442 a          253 c          201 g          477 t
ORIGIN      
        1 atcgtttcta tcattttgat ttcaaatata gccggccata atttaccggt aacaaaaagc
       61 ctgttatttt ccgcatcata ggcaatacca ttcaaaacat ccacattttc atggtgttga
      121 acattatcta atataccata taagtcaagc cagaatacta tctttcctgt gcctggctca
      181 attacagcaa ttctatcgct ttgccagata ttggcatata tgtaaccttc aatatattcc
      241 aactcattga tccaattcac taaaaacagt ccatcatgga ctttaacctg ttttatctca
      301 tctaaagttt tcggatccaa aaaatgtaaa acaggggttc catcacttat tattaaaaaa
      361 tctccatcag tggttattcc ccatccttcg tagggataag aaaaggtatc taaaagttga
      421 aaagtatccc gggtatagat aaaccccatc tcttccctcc aggttagcag gaatatctta
      481 tcatcaaaaa tagtaatccc ctcagcaaaa taattatcag caagaggaat attcttaatt
      541 gctttgccag tctttaattc gacctggcgt aaagatgaag agccatacaa accggtactt
      601 tcatagagat agccatcatc ccatattagt ccctgggtaa aagcttcagg atcatgggga
      661 aaaatattta caatttcaca agataggatt ggggtggtat tatcattgtc attaatatta
      721 gttgaattac ttgaacagct ctgaactgac agtgataaaa aaataatcac tatcaataac
      781 aaacatgtca ggggacggtc ctttagcaac attgatatca cttttctctc ctaatatttt
      841 gaattattcc tctagttatt cccaggaatt ctgctatctc tttatttgaa aaaattgatt
      901 catcttttaa ttttaaaatt aactggtccc taatcgattt gttttctctt ttaaacaggt
      961 cttgtaaaaa tattttgtgt tcatctacaa atttattaac aaaattggta aattttttct
     1021 cagtatttat ttgtttgggc tgctcatttt tatctttata ttctataaat ttatgagttg
     1081 tctcctcttt ttgtgagaac cctttgaatt gctccttagc ttcttcaatg tcataagaga
     1141 atatcttaag gatgtcttcg atatcaagaa gcttgtaaat attttttccc gggaaaaaat
     1201 aataaaaaca tgaactccag gggtattctt cgggagacag ggctattcca gctttaacag
     1261 ggttctggtg aatatatctg ataagagaaa ttaaataagc atcattctca acattttcac
     1321 tcttaaatcg attctgaaat aaatgtccgg caagttgata ttttttatta aaa
//
LOCUS       DAOQWT010000466         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_123726_length_1367_cov_3.403201, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000466 DAOQWT010000000
VERSION     DAOQWT010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123726_length_1367_cov_3.403201"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKJ95_07935"
     CDS             <1..322
                     /locus_tag="PKJ95_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNR66203.1"
                     /translation="MAIDSEKLIPRLGNKVGGISGPGIRPIIVRMIWEASQRVKIPIV
                     GVGGITSTRDALEMFLAGASAVQVGSANFSNPYVMLNIIEGIAGYLNKKKYKSVKDIV
                     GLIK"
     gene            387..1256
                     /locus_tag="PKJ95_07940"
     CDS             387..1256
                     /locus_tag="PKJ95_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR66204.1"
                     /translation="MGLGLQMFVNALLLGGLYALMGIGFSLQWGISGIINLSYGAMVL
                     LGSYLSLIIFNMFHIDPFISMFVTGAILFLLGAFIYRFTLQPLLKGGIVFTLIVTFAI
                     RLIIENIILLVWSADYRTIRVSYAGSNFQFLGAFVPLTKFLTFIIAGVIIYLTYQFMM
                     NTKIGKGIQAVALDKEGAMAVGVDDQKMYLINFALGTALGGVTGTLWATIYSFSPHVM
                     DVIIGRVFIIAILGGLGNIWGAAAGGLLLAFVETSGAIFIGSHWQEAIGLVIMVLVLL
                     LRPHGLIGKKFFG"
BASE COUNT          389 a          208 c          322 g          448 t
ORIGIN      
        1 tatggcaata gattcagaaa aactaatacc gaggttaggg aataaggtgg gggggatatc
       61 aggtccgggc attagaccga ttattgtgcg gatgatctgg gaagccagcc aaagggtgaa
      121 aataccaatt gttggggtag gaggaattac cagcacccgg gatgccctgg aaatgttctt
      181 agcaggagct agtgccgttc aggttggaag tgctaatttt agcaatcctt atgtaatgct
      241 aaatattatt gaagggattg cagggtatct caacaaaaaa aaatataaat cagtaaaaga
      301 tattgttggt ttgataaagt agtgttctat atacttttgt tctatttata actattgcta
      361 aaattgaaaa ttcaaggagg aattaaatgg gattgggttt gcagatgttc gtcaatgcgc
      421 ttcttttagg ggggttgtat gcattgatgg gaataggatt ttctttgcaa tgggggataa
      481 gcggcattat taatctatcc tatggcgcta tggtattgct cggttcttat ctctctttaa
      541 tcattttcaa tatgtttcat atagacccct tcatcagtat gtttgttacc ggggctatat
      601 tgtttttatt aggtgctttt atatatcgat tcacacttca gcctctcctt aaaggcggta
      661 ttgtctttac tcttattgtt acttttgcca tcagattaat aatagaaaat ataatacttc
      721 tggtctggtc tgcagactat cgtactatca gggtaagcta tgccggaagc aatttccaat
      781 ttttaggagc ctttgttcct ttaactaaat ttttgacctt cataatagcc ggggtgatta
      841 tctatcttac ctatcagttt atgatgaata ctaaaattgg gaaagggata caggcagtcg
      901 ctttagataa agaaggagcg atggcagtag gggttgatga ccagaagatg tatttaataa
      961 attttgccct gggcactgcc ctgggaggag ttacgggaac cctatgggca accatttaca
     1021 gcttttctcc tcatgttatg gatgtaataa taggcagggt ctttattatt gccattttag
     1081 gggggttagg gaatatctgg ggagctgctg ccggtgggct attattagcc tttgtagaaa
     1141 catcaggggc catctttata ggctctcact ggcaggaagc tatcggactg gtcataatgg
     1201 ttctggtatt actattaaga ccacatggtt taatagggaa gaaattcttt ggttagtcat
     1261 tattagggaa ctatttttaa aggaaggaag tgtgagataa gtgatagcac aaaatgacag
     1321 aaaattaaat ataaacataa ttctgattgt tgctgccctt ataatcc
//
LOCUS       DAOQWT010000467         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_124625_length_1361_cov_4.110260, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000467 DAOQWT010000000
VERSION     DAOQWT010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124625_length_1361_cov_4.110260"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1361)
                     /gene="leuS"
                     /locus_tag="PKJ95_07945"
                     /pseudo
     CDS             complement(48..1361)
                     /gene="leuS"
                     /locus_tag="PKJ95_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082796.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
BASE COUNT          397 a          301 c          239 g          424 t
ORIGIN      
        1 aaatcaaaag gtaacgtggt tacacccggc aatattttta atcagtatgg tgtaaccacg
       61 ttaccttttg atttagacat tacagcccct tccttacaga ccataccctg ggtgaagagg
      121 cgtttgaaag gttctttaaa tttcagataa cctatatcgt agagagcttt aacaaaaaat
      181 cgggaataca agaggtgtag gatagcatgt tctactcccc ctatgtattg atctaccggc
      241 atccaataat ggagttcatt ttcatcaaag ggtttgttat tcagatgtgg tgagcaaaaa
      301 cgaaggtaat accaggaaga acagacaaaa gtatccatag tatcggtgtc tctcttagcc
      361 tctcctccac atttaggaca ggttgtatat aaaaattctt tactgtaaag caaaggggat
      421 aagccagtcg gtttaaaatc aacatcttgt ggcagataaa ccggtaaatc tttttccggc
      481 acgggcacca ttccgcattc aggacaataa acaataggta ttggggcgcc ccaataacgt
      541 tgtcgggaga ttaaccaatc ccttaaacga tagttaattt ctcgtttgcc taaaccctct
      601 ttttctagat attcaattat tatttctttt cctttatcac tgggaagccc attaaatctt
      661 cccgaattaa ccatgatacc ttctagatca aatgcctcct gtaggttgtc ttctttcagg
      721 tggaaatttt ccggttgaat aactattctt accggtaaat tatattttcg ggcaaattct
      781 aaatcccttt ggtcgtgtgc cggcactccc ataaccatac cggtaccata ttccatcagc
      841 acataattag ccagccagat tggaactttt tcttgattta atgggtttat aatatatcga
      901 ccggtaaaac agcctctttt ttctgttaaa tcaatattct tctcattaat tttttcttta
      961 aagaattctt ctttgaattt cattacctct tcttcataat cggtacctcg aaccaattgg
     1021 tctactatgg gatgttcagg agaaagaaca aaaaaggtta ccccgcagag tgtatcaggt
     1081 ctggtagtaa aaacagtgaa ggtctggcct gttcccgcca ggataaagtt tacctgagta
     1141 ccctcacttc tgccaatcca attcctctgc attgttaata cccgttcagg ccattcttct
     1201 aaaatatcca tatcatctaa aagccgctgg gcataatcgg ttatcttaaa aaaccactgg
     1261 gacaattctt tttgggtaac ctcggttcca catctctcac aacccccgtt aacaacctgc
     1321 tcatttgcta atactgtggc acaagaaggg caccagttaa c
//
LOCUS       DAOQWT010000468         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_124709_length_1361_cov_2.169219, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000468 DAOQWT010000000
VERSION     DAOQWT010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124709_length_1361_cov_2.169219"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1361
                     /locus_tag="PKJ95_07950"
     CDS             <1..>1361
                     /locus_tag="PKJ95_07950"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNR66205.1"
                     /translation="EFLNENIAIEQINFKNSLLVYKDEAVYHKDNITVKTKDLNGSFD
                     LTHLPEIKFEMRAIQAPDEAILALSGFFFVGKIEYSLDFHLQNADITHFQYYLQAAEQ
                     FNISKGKFDLELNLSHSPEWDSDVLFWQGDATFYEANAQPYFLKEMPFQQISGSINFS
                     KPEIIISNLTGLHYDHIIQLEGQVQTAPETSYNLKIEGKHLEASLLKDDISLFTSENY
                     DFTLEGEIDLWGNINGLTDAFSFQGKINSSEIIIEETPFSTISGNFTLNNKGLIINDL
                     KSQDSNGSILIDGNINWDQDLPSYQFIIKTSDLSLHHSLFKQFSALQDFSGNMESQFQ
                     IESQIPDSAKSSLSGIFSINKIKKGDFALPDPLKGNMEVIIDLSDNRFSIEKCELQSG
                     GSSGSVKGDIRFEEMANFTLDFNYQIPDLTTYGPAFQKDLKIAGNLALQGKAKGNILQ
                     PEI"
BASE COUNT          445 a          227 c          253 g          436 t
ORIGIN      
        1 agaattcctc aatgaaaata tagccataga gcagataaat tttaaaaata gtctactggt
       61 ttataaggat gaggcagtat accataaaga taatattact gttaaaacaa aagaccttaa
      121 tggttctttt gatttgactc atctgccaga aattaaattt gagatgaggg ctattcaggc
      181 tcctgatgag gctatcttag ctttaagtgg attctttttt gttggtaaaa tcgaatattc
      241 tctggatttt catttacaaa atgccgatat tacccatttt caatattacc tccaggcagc
      301 tgaacagttt aatatcagta aaggaaagtt tgacttagag ctcaatctaa gccattctcc
      361 cgaatgggat agtgatgttc ttttctggca aggcgatgca actttttatg aagctaatgc
      421 tcagccttat tttttaaagg aaatgccttt ccagcaaata agtggttcta tcaattttag
      481 caaaccagaa atcataattt cgaatcttac cggccttcac tatgaccata ttattcaatt
      541 agagggacag gttcagactg ctccggaaac cagctataat ttgaagatag aaggaaagca
      601 tttagaagcc tccttgctca aagatgatat ctctcttttt accagtgaga attacgattt
      661 tactctggaa ggggaaattg atttatgggg gaatattaat ggcttgacag atgccttctc
      721 ttttcaggga aagatcaatt catctgaaat aattatcgag gagacaccat tttctactat
      781 ttcaggaaac tttactctga ataacaaagg gttaattata aatgatctga aatcccagga
      841 ttcaaatggt tctattttaa ttgacggtaa tattaactgg gaccaggatt taccctcata
      901 ccagttcatt attaagactt ccgatctatc tttacaccat tcattattta aacaattttc
      961 agctcttcag gatttttcag gaaatatgga aagccagttt caaatagaaa gtcaaatacc
     1021 agatagtgct aaatccagtc ttagcgggat tttttctatt aataagataa aaaaaggaga
     1081 ctttgctttg cctgaccctc tcaagggcaa tatggaggta ataattgacc tttctgataa
     1141 taggttttca atagaaaaat gtgaattaca atcgggcggg agcagtggat ctgtaaaggg
     1201 tgatattcga tttgaagaaa tggctaattt tactttagat tttaattacc agattcctga
     1261 tttaacgaca tatggccctg cattccagaa agatcttaaa atagctggga atttagctct
     1321 gcagggtaag gcaaagggaa acatccttca gccggaaatt g
//
LOCUS       DAOQWT010000469         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_124772_length_1360_cov_5.146360, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000469 DAOQWT010000000
VERSION     DAOQWT010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124772_length_1360_cov_5.146360"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..680)
                     /gene="tnpA"
                     /locus_tag="PKJ95_07955"
     CDS             complement(273..680)
                     /gene="tnpA"
                     /locus_tag="PKJ95_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018404404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNR66206.1"
                     /translation="MKLKAHYHCVYSLNYHLVLVTKYRKKCFTPEILDFLKEIFKKLS
                     EKWEIELLEFNGEADHIHLLIATHPNIKMSSLINNFKTVSSRLVRKKFSAHLSQFYWK
                     PVLWTRAYCLISTGGATIGVIEKYIQNQGLEKK"
     gene            746..>1360
                     /locus_tag="PKJ95_07960"
     CDS             746..>1360
                     /locus_tag="PKJ95_07960"
                     /inference="COORDINATES: protein motif:HMM:NF023742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR66207.1"
                     /translation="MMKTFKYRIYPGKSQESKLENTFSMCRHLYNRSLTERITSHQVW
                     KNAKDIEKVINPDKKKIIFSSSMGQVFYSKEKYLTAIKLLTTVESVFGIQFTSIWCNV
                     PVKVDYYSQAENLPLLKKERPWYKGVHSQVLQNVLKRLDDAYDGFFNEGKGYPKYKKR
                     GEYSSITYPQYVIRPEEKKLTVSKIGAIKIVYHRAIPKEARLKTL"
BASE COUNT          494 a          209 c          228 g          429 t
ORIGIN      
        1 catcctgagc gaagtcgaag gatgttcgaa aaattttgcg taagtcctga taaaattcag
       61 atttaacata aatggaaaaa ataatttatc ctaaagaccg cagaatactc cctctatagc
      121 tgaaggctta gagggagatg aatgcggagt tactcctttt agctatattt ttacagatat
      181 cccaccctct aaaaatttca aaataaaata gagatatgat agtaatttgc gtatctacta
      241 cataaaaaat taagtaatac aagtatttta agctattttt tttccaatcc ctggttctga
      301 atatatttct caattactcc tattgtagct cctccggtag atatgaggca gtaagctctt
      361 gtccatagaa ccggtttcca atagaattga gataaatgtg cagaaaattt ttttctgacc
      421 agtctacttg aaacagtttt gaaattattg ataagtgaag acattttgat atttgggtga
      481 gttgcaatta aaagatgtat gtgatctgct tcaccattga attctaatag ttctatttcc
      541 catttttccg atagtttttt gaatatttct ttgagaaaat cgagaatttc aggagtgaaa
      601 catttttttc tatatttcgt aaccaacact aaatgataat ttaaactgta tacacaatga
      661 taatgagctt ttaatttcat gtaattcacc ttgacactaa ataatatata ttttataata
      721 atatcacagg gagatgttca ggtcaatgat gaaaacattc aaataccgta tatacccggg
      781 aaaatcccag gaaagtaaat tagaaaatac cttcagtatg tgccgtcatc tctataaccg
      841 ttcacttact gaacgtatta cttctcatca ggtatggaag aatgcaaaag acatagaaaa
      901 agtcataaat cctgataaga agaaaattat cttttcttct tcaatgggac aggttttcta
      961 tagtaaagaa aaatacttaa cagcaataaa gcttcttaca acagtagaga gtgtattcgg
     1021 tattcagttt acgtctatct ggtgtaatgt gccggtaaaa gtagattatt acagtcaggc
     1081 tgaaaatctt ccgttattaa agaaggaacg tccctggtat aaaggagttc attctcaggt
     1141 attacagaat gtattaaaga gactggatga tgcttacgat ggttttttta atgaaggaaa
     1201 aggatatcca aaatataaaa aacgtggaga atatagttca ataacctatc ctcagtatgt
     1261 aataagacct gaagaaaaaa aactaacagt gtcaaaaata ggagcaataa aaatagtcta
     1321 tcatagagct ataccgaaag aagccaggct aaaaaccctg
//
LOCUS       DAOQWT010000470         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_124929_length_1359_cov_3.395706, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000470 DAOQWT010000000
VERSION     DAOQWT010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124929_length_1359_cov_3.395706"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1165)
                     /locus_tag="PKJ95_07965"
     CDS             complement(41..1165)
                     /locus_tag="PKJ95_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNR66208.1"
                     /translation="MKKNQKIAIYILILTAIVIILSALEFVDQWKSDLIVAGNGVTRM
                     AKLSDYYPELRGEYCDTDIYFLESNEPGGTVFVLGGTHPNEPSGMLSVVLLIENAVVT
                     KGRMIVIPQANLSGYSHNDPQEGSPRKIHFNTPYGRREFRYGSRATNPIHQWPDPDIY
                     INQFGQSLSGSETRNLNRGYPGDPEGNYTEKICYGILEVIKKEECDLSIDLHEASPEY
                     PVINAIVAHERAIDLASIAVLDLQMEDLDYNLEISPPSFRGLTHREWGDYSDTMAILM
                     ESANASQGRIRGRTDEALVLTGKDKMYVKAAELGRLYVPYDEKGHPIEERVGRHLTAI
                     GVLVENMSLLFPDETVEYYGIPSYQELLQYGYGNYLQPVK"
BASE COUNT          414 a          294 c          215 g          436 t
ORIGIN      
        1 ctttttatcc actttttaaa ttttgttcat cctataatta ttattttacg ggctgaagat
       61 aattgccata accatattgt aacaattcct ggtaagatgg tattccataa tattccaccg
      121 tttcatcagg aaataaaagg ctcatatttt cgaccagtac tccaatagca gtgagatgtc
      181 ttcctaccct ttcctcaatg ggatgcccct tctcatcata tggtacatat aaccttccca
      241 gctcagcagc ttttacatac attttatctt tgccggtcaa gaccagggcc tcatctgttc
      301 ttccccttat tcttccctgg ctggcatttg ccgattccat caaaatagcc atagtatcac
      361 tataatctcc ccattctcta tgagttaatc ccctaaaaga gggcggggat atttccagat
      421 tgtaatccag atcctccatt tgtaaatcca aaacggcaat actggctaaa tctatagccc
      481 tttcatgggc aacaatagca tttataaccg gatattcggg tgaagcttca tgtaaatcta
      541 ttgataaatc gcattcttcc tttttaataa cttccaaaat gccataacag attttttcgg
      601 tataattacc ctcaggatct ccgggataac cacgatttaa atttcgcgtc tctgaaccgg
      661 aaagtgattg accaaactga ttgatatata tatccgggtc aggccactga tgaatagggt
      721 tagtagccct agaaccatat ctgaactccc ttctgccata aggtgtatta aaatgaatct
      781 ttcgaggtga accttcctgc gggtcattat gggagtaacc gctaagattg gcttgcggta
      841 ttacaatcat tctaccttta gttaccaccg cattttcaat taatagcacc acagaaagca
      901 tgccggatgg ttcattggga tgtgttcctc ctaacacaaa aacggtacct cccggttcat
      961 tactttctaa aaaatagata tcagtatcac agtactctcc ccttaattca gggtagtaat
     1021 cacttaattt agccattcta gttacaccgt ttccggctac gattaaatca gacttccatt
     1081 ggtcaacaaa ttccagagca gacaatatga taacaatggc agttagaatt aatatataga
     1141 tagcaatttt ttgattcttt ttcataggca aaacctcttt ttctggcact aattactgac
     1201 aaaatataca ttttttaaaa tgttatttta tcataaaaaa tctcaggata aagggaataa
     1261 tgaccaggga gctggtaagt tgattccaga aatcactctc tttaaacata tcctgcacga
     1321 ttaagactgt tagaaataaa atgattaaac gataaatta
//
LOCUS       DAOQWT010000471         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_125080_length_1358_cov_3.890253, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000471 DAOQWT010000000
VERSION     DAOQWT010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125080_length_1358_cov_3.890253"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="PKJ95_07970"
     CDS             <1..1234
                     /locus_tag="PKJ95_07970"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNR66209.1"
                     /translation="NFPSGEMIEARILFPPSLVPNTPKIISENRFDKIMAEELNWAKS
                     SQRQQSAIFLALALIPFIVIINIVMAIRFYFKYDRELKPEMKLDYYRELPEDITPAVL
                     SQLINLQGVTTKEIMATLMDLTRKGYLKIEEEGAGRKKDYRFILINRNFGGLKNHELN
                     LINWLISIIGNGKDISLKEIENYSKKTAKSSWTRQSFYQQYTSWQKTVRDEFRKYNYF
                     NEGKPGLKAALKTISLEIATIFVLIFLAILFKMPWYAFIPLLVVTGLTGVGLIIYGAV
                     IRKKTQKGVNEYHKWMAFKRFLLHFSNMKDYEIPSLVVWEHYLVYAITLGVADKVISK
                     LRPILAAQNINMGNTAFLYHMTSSNGQLNAATFSSFDRAFSSAFTHVSPSTGSGGGFS
                     SGGGGGGGGGGGGGAGAF"
BASE COUNT          424 a          220 c          288 g          426 t
ORIGIN      
        1 gaattttcca tctggtgaaa tgatagaggc aagaatttta tttcctcctt ctttagttcc
       61 caatacccct aaaatcatct ctgaaaatcg ttttgataag attatggcgg aagaactaaa
      121 ctgggcgaaa agttcacaac ggcagcagag tgctattttt ttagctctgg cactcattcc
      181 tttcattgta ataataaata ttgttatggc aatcagattc tactttaaat atgaccgtga
      241 acttaaaccg gaaatgaagc tcgattatta tcgggaatta ccggaagata tcactcctgc
      301 tgttttgagt cagttaataa acttacaggg ggtaactacc aaagaaataa tggcaaccct
      361 gatggatctg actagaaaag gatatttaaa aatagaagaa gaaggtgcag ggagaaagaa
      421 agattatcgc ttcatattga taaaccggaa ttttggggga ctgaaaaatc atgagctcaa
      481 tttaattaac tggctcattt ctattattgg taatggaaag gatatttctt taaaagagat
      541 agagaattat tctaaaaaaa ctgcaaaatc atcctggacc cggcagtctt tctatcaaca
      601 gtacacaagc tggcaaaaga cagtgagaga tgagtttaga aaatataatt actttaatga
      661 gggcaagccg ggtttaaaag ctgctttgaa gacaatctct cttgagattg caaccatatt
      721 cgtattaata tttttagcca tattatttaa aatgccctgg tatgccttta ttccgttatt
      781 agtagtaaca ggactaacag gggtgggatt aattatctac ggggcagtta tcaggaaaaa
      841 gactcaaaaa ggtgttaatg aatatcacaa atggatggct tttaagaggt ttttgctcca
      901 tttcagtaat atgaaagatt atgagatacc atctctggtg gtctgggagc attacctggt
      961 ctatgctata accttaggtg ttgctgataa agttatttcc aaattaagac cgattctggc
     1021 tgcacaaaat atcaatatgg ggaacactgc ctttctctat catatgacca gcagtaatgg
     1081 ccaattaaat gcggcaactt ttagctcttt tgacagggct ttcagcagtg cttttaccca
     1141 tgttagccct tctaccggtt ctggtggagg attctcttcc ggtggtggcg gaggtggcgg
     1201 cggaggtggc ggcggtggag ccggagcctt ctgatatttc aatatactga taaacgatta
     1261 tctttgtatc atatcatata ttttaaaaaa ggagtaataa tatgaatttt gttttaatct
     1321 ttttaatact gattgttttg tggggggtag tggcctat
//
LOCUS       DAOQWT010000472         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_126353_length_1350_cov_3.083398, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000472 DAOQWT010000000
VERSION     DAOQWT010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126353_length_1350_cov_3.083398"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKJ95_07975"
     CDS             complement(<1..540)
                     /locus_tag="PKJ95_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNR66210.1"
                     /translation="MVRDSSSRKIKIIRIEDKKRLSITDLTAREVELTILINNRKLIS
                     LNCSPGNYQFLAVGFLYTNGIITGREDIVSIKTGENKVDITLNNTVMPVEKEINLHLP
                     IGFLKPGTVAESKKLYDKEKRIIIKSDTIFTLLNHLQEKAFLFNKTGGVHSCALADRE
                     GSLLLFCEDVSRYNTVDRIL"
     gene            complement(527..1177)
                     /locus_tag="PKJ95_07980"
     CDS             complement(527..1177)
                     /locus_tag="PKJ95_07980"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNR66211.1"
                     /translation="MFKEKKHTPFSVLVLAGGRSSRMGLNKDKGHMQFKGTSLIEYVL
                     VNILSIEGVDKNNVIIVGPKEKYSRHGFTVVEDLYPQKGPLGGIFSGLQYSNTFYNLV
                     IGYDMPFVESKLVEYMLSNSKGYDMVIPTYGKELYEPLCAIYSKNCLKTMEQNLKNNI
                     LAVRGIFPRCKIRWIREEEIRIFDSELHSFFNINFQSDFKKAEILLYQRGKNIGKG"
BASE COUNT          392 a          259 c          209 g          490 t
ORIGIN      
        1 cagtatacgg tctacggtgt tgtaacggct tacatcttca cagaataata acagggaacc
       61 ctccctatct gccagagcgc aactatgtac gccaccagtc ttattaaaca aaaaagcttt
      121 ctcttgcagg tggttgagga gagtaaaaat ggtatctgat ttaatgatga ttcttttctc
      181 tttgtcatat aatttttttg attctgccac tgttcctggc tttaaaaagc ctatgggcaa
      241 atgaaggtta atctctttct caactggcat cactgtatta tttaatgtta tatcgacttt
      301 gttttcacca gtttttatag atactatatc ttctcttcca gttattatgc cattagtata
      361 taaaaaacct accgctaaga attgataatt ccccggactg cagtttaagg atatgagctt
      421 acggttattt atcagaattg ttaattcaac ttcccgggca gttaagtctg ttatactgag
      481 tctcttctta tcttctatac gaattatttt tattttcctg cttgaactat cccttaccaa
      541 tatttttacc tctttggtag agcagtattt ctgctttttt aaaatcgctt tgaaaattaa
      601 tgttaaagaa agaatgtaat tccgaatcaa atattctgat ctcttcttct ctgatccagc
      661 gtattttaca acgagggaaa attcccctta ctgccaatat attatttttt agattctgct
      721 ccatagtctt caggcaattt ttactataaa tagcgcataa aggttcatac aattccttac
      781 cgtaggtcgg aatcaccata tcataaccct tactattgct aagcatatat tcaactaatt
      841 ttgactcaac aaagggcata tcgtatccta ttaccaggtt ataaaatgta ttcgaatact
      901 gtaagccgga aaaaattccc cccagtggac ctttttgtgg gtagaggtct tctaccacag
      961 taaatccatg tctggagtac ttttcttttg gtcctacaat aataacatta ttcttgtcta
     1021 ctccttcgat agataggata ttgacaagaa cgtattctat cagactggta cctttaaatt
     1081 gcatatggcc tttatcttta tttaatccca ttctactgct tctgccaccg gctaagacta
     1141 agactgaaaa gggagtgtgt tttttctctt taaacatttt ctttactcat taatctgggt
     1201 taatggttag caataattaa gaagatttct gataggtcaa aagaggggct atgccatatc
     1261 tgctgcgaca gtataacccc tcctgattca tttaattgag tttataaact atttatttaa
     1321 taaagtaaaa attattcgaa ttgattgcca
//
LOCUS       DAOQWT010000473         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_127058_length_1346_cov_2.301317, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000473 DAOQWT010000000
VERSION     DAOQWT010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127058_length_1346_cov_2.301317"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PKJ95_07985"
     CDS             complement(<1..659)
                     /locus_tag="PKJ95_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66212.1"
                     /translation="MIIYLALVSCLLFSKTNSSASDTIIARSLSLKDVQAAIKKAKIG
                     DTVIVPNGTARWTSALIIFKGIILKAETPGGVKIINDISVKGKYHYFNPENYVIAYCP
                     EDEKTGQPFRLSGFVLDGGKNRWLFLGKTRSATMVITQFRVDHCVFQNGYRIPINFKG
                     EIYGVLDNNVIHGSTREFAFNKDTWENLTFNFGTESNRYYEDNVIYTYAGDTAPEGGL
                     GG"
     gene            885..>1346
                     /locus_tag="PKJ95_07990"
     CDS             885..>1346
                     /locus_tag="PKJ95_07990"
                     /inference="COORDINATES: protein motif:HMM:NF025755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HNR66213.1"
                     /translation="MKELFHYLNSGCFIIQETVIQHPEMSRLNASSLNTIRIDTYNSF
                     NEPPKVISALFRMGRSGSAVDNAMSGGIFVGIDMETGKLRSNAFAKLEHGGIVYSKHP
                     DSGVPFKDFTIPYFSEVKEMACQAASSLADKLVGWDIGISAEGPVLIEGNGH"
BASE COUNT          402 a          281 c          249 g          414 t
ORIGIN      
        1 ccaccaagcc ctccctccgg agcagtatct ccagcataag tgtaaatcac attatcttca
       61 taataccggt tgctctctgt tccaaaatta aaggtaagat tttcccaggt atccttgtta
      121 aaggcaaatt ctctggttga accatggata acattgttgt caagaacacc atatatttca
      181 cctttgaaat tgatgggtat tcggtatcca ttttggaaaa cacaatggtc aacacgaaac
      241 tgagttatca ccatagtcgc tgaacgagtc ttgcccaaaa aaagccacct attcttcccc
      301 ccatcaagaa caaatcccga aagcctgaag ggctgacctg ttttttcatc ctcagggcaa
      361 taagcaatca catagttctc tggattaaaa tagtgatatt tacctttaac tgaaatatca
      421 ttgattattt ttacaccccc aggtgtctct gccttcaata tgataccctt gaaaattatc
      481 agggcactcg tccaccgtgc tgtcccgttt ggcactatta ccgtatcacc tattttggct
      541 ttttttattg cggcctgaac atctttgaga gaaaggctcc tggcaataat cgtatctgag
      601 gcagaggaat tcgtttttga aaacaacaaa catgaaacca gagctaaata aattatcatc
      661 aagctgcggt aactggctta ttttttatac tcattaattt cctctttccc caccttggtg
      721 actcttttca agagataaaa aacctttcta aatggcctac ttccttttca ttaacttttg
      781 atgaagatat aactataagt aatagataaa ggaaaacatg tcaagtaggt aaagttgcta
      841 caaagatttc acaggaaata ttttttcccg ctcaatccga aaaaattaaa gaattattcc
      901 attatctaaa tagcggttgt tttattattc aggaaactgt tatccagcat cctgaaatga
      961 gccggcttaa tgcttcaagt cttaacacaa ttaggattga tacctataat tcatttaatg
     1021 aacctcctaa agtaatatct gcactttttc gaatgggtcg ttctggaagt gccgtagata
     1081 atgccatgtc tgggggcatt tttgttggca ttgacatgga aacaggcaaa ctgcgctcta
     1141 atgctttcgc caaactggag catggtggca tcgtatatag taagcaccca gatagtggtg
     1201 ttccgttcaa ggattttacg attccttatt tctccgaagt caaagagatg gcttgtcagg
     1261 cggcaagttc tcttgccgac aaattagtcg ggtgggatat agggatttca gcagaaggac
     1321 cagttttgat agagggaaat ggccat
//
LOCUS       DAOQWT010000474         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_128459_length_1337_cov_3.258190, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000474 DAOQWT010000000
VERSION     DAOQWT010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128459_length_1337_cov_3.258190"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            411..>1337
                     /locus_tag="PKJ95_07995"
     CDS             411..>1337
                     /locus_tag="PKJ95_07995"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNR66214.1"
                     /translation="MELLLKQTKRKIVKIPGTKIIDRYIISQVLSYFLGMVALLTILL
                     IVETMFELMEMLINKKVPIISVIELLVYRLPAFLAVTFPVALLGSSELAIAQMSVNNE
                     IAAMRTGGISFRRIMKPFLITGILISILSFATNDYIVPEANHISQNIIREIVLKKGPP
                     NIQRNVFFRDAENRYFYINRLDEQNMIMREIMIYEMTRERFPRVITASEGRWVVDTWL
                     LKNGTIYNYNSEGKITYEMTFQDMEIEVKEDLQDFFKNQRTPQEMSSKELKQQIQILK
                     DAGVDTKNFEVDYHVKYSFPFSGLIFALIGVPL"
BASE COUNT          443 a          219 c          222 g          453 t
ORIGIN      
        1 cttttataat tccctaatgc ttctttagct ctaataaaaa ttgcttctct ttttgcatct
       61 atgagttgcc tttctattac actcacagcg tattctctat cctctaatga taaattatta
      121 aattcttcta tcaacttatt taatgattct gttttcaaat gaaaaacctc caatcagtgg
      181 attaaaagcc tttctaaaaa agataaatat tatcttgact ttatctccaa gtaccttatg
      241 attttatgtt taaaatttct gatacactaa tttagcatat aaatatatag atttcaacag
      301 ttaaatcaaa cagaaattag ttaaatcatt tttttataaa aaatattttt gtgttatact
      361 aatatataaa ttttataaac aagaatgtag cttgaagaag tttatcaaaa atggaattat
      421 tattaaaaca aacaaagagg aaaatagtga aaattcccgg tacaaaaatt atagaccgct
      481 acatcatttc tcaggtatta agctatttcc tgggaatggt tgccctttta acaattctac
      541 ttattgtgga gacaatgttt gagctgatgg agatgctcat taataaaaaa gtgccaatta
      601 ttagcgtaat tgaattgttg gtttatcgtc ttcctgcatt tttggcagtt accttccctg
      661 tcgctctttt aggctccagt gagctggcta ttgcccagat gtctgtcaat aatgagattg
      721 cagccatgcg taccgggggc atcagttttc gtagaattat gaaacccttc ttaattacag
      781 gtatcttaat ctctatttta tcctttgcca caaatgatta tatagtaccg gaagccaatc
      841 atatttctca aaatataatc agggaaattg tactaaaaaa aggccctccc aatattcagc
      901 gcaatgtctt tttccgtgat gctgaaaaca gatatttcta tatcaaccgt ttagatgaac
      961 agaatatgat tatgagagaa ataatgatat atgaaatgac ccgggaacga tttccaaggg
     1021 ttatcactgc cagtgaaggt cgctgggtgg tggatacctg gcttttaaag aatggtacta
     1081 tctacaatta taatagtgaa ggtaaaatca cctatgagat gacttttcaa gatatggaga
     1141 tagaggtgaa agaagatctt caggacttct ttaaaaatca gcggacacca caggagatga
     1201 gcagcaagga gttgaagcag caaatacaaa tattgaaaga tgccggagtg gatactaaaa
     1261 attttgaagt agattatcat gtgaaatact cctttccttt ctctggcttg atttttgccc
     1321 tgattggagt acctctt
//
LOCUS       DAOQWT010000475         1336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_128607_length_1336_cov_7.145199, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000475 DAOQWT010000000
VERSION     DAOQWT010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128607_length_1336_cov_7.145199"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PKJ95_08000"
     CDS             complement(<1..1322)
                     /locus_tag="PKJ95_08000"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR66215.1"
                     /translation="MKAEKMYFDSVSRDRMLSDKFGLEGIDSDGRKANIKDNDRDSTS
                     TMPDINLLPSSGTIEPNSSVTCILNFIPKDTPGTYNYELQLSSNDPVSPLITVPLKYV
                     VNSPIAYIPDDNFRMAINDALGQPSYYQPTIADLQGLTGYLYAEGRNIISIEGAQYFT
                     NLDSLYLSYNQISNLNPLAGLTNLKGLNLGSNQISDINPLAGLTNISWLFLFGNYISN
                     IAPLEGLYNLRNLQLHYNQISDITPLAALIDIGGIALGSNQIVDITPLAGLTHLIGLE
                     LYHNQINNIDALSGLINLQWLYLDGNQIIDISPLAGLSILRELYLENNQINDISTLSE
                     LNNLQYLFLYNNQISDLSPLAGLTNLWWLLLDGNPISYESMLLAQSWSLPWSTTSYNS
                     LSPCYPDPNRGATNVLVNYPLSWQGNYENSPDVSYEVWLGISPDSLVY"
BASE COUNT          443 a          235 c          232 g          426 t
ORIGIN      
        1 tgatagacaa gtgaatctgg gctaatccct aaccaaacct catagcttac atctgggcta
       61 ttttcataat tcccctgcca ggataaagga tagtttacta aaacattggt agcacctcta
      121 ttcggatcag gataacaagg agaaagagaa ttgtaacttg ttgttgacca aggtaaagac
      181 caactctgag caagtagcat tgactcataa gatataggat taccatcaag caacaaccac
      241 caaagattag tcaaccccgc taagggacta aggtcactta tctggttatt atatagaaat
      301 agatattgca gattatttaa ctctgataaa gtgcttatat catttatctg gttattttct
      361 aaatataact ctcgtagaat acttaatcct gccaaggggc taatgtcaat tatctgattt
      421 ccatcaagat ataaccattg taaatttatt agtcctgata aggcatcaat attatttatc
      481 tgattatgat aaagttcaag tcctattaaa tgagtaagcc ctgccaaagg agtaatgtca
      541 actatttgat tactgccaag tgctatacct cctatatcaa tcaatgctgc taaaggagta
      601 atatcactta tttggttata atgaagctgc agatttctta gattatataa tccttccaaa
      661 ggagctatat tactaatata attaccaaaa agaaataacc aactaatatt tgtaagcccg
      721 gctaagggat tgatatcact tatctggtta ctgccaagat tcagaccttt tagattagtt
      781 aaaccagcta agggattgag attacttatt tggttataag acaaatacag agaatccaaa
      841 tttgtaaaat attgagcacc ttctatgcta attatattcc gaccctcagc atacaagtat
      901 cctgttaatc cttgtagatc agctatggta ggttgatagt aagagggttg acctaaggca
      961 tcattgattg ccattcggaa attatcatca gggatatagg caatggggct gttaactaca
     1021 tatttaagag gaactgtaat aagaggtgaa acaggatcat tggatgagag ctgaagttca
     1081 tagttatagg tgccaggtgt atctttagga ataaaattca ggatgcaggt tacagaagaa
     1141 ttgggttcta tagttccgga agaaggcaaa aggttaatat ccggcattgt ggaggtgcta
     1201 tctctatcat tatcttttat atttgccttt ctgccatcac tatctattcc ctctaaacca
     1261 aatttatcag acaacattct atctctactt acactatcaa aatacatttt ttctgctttc
     1321 attctttcta tctcag
//
LOCUS       DAOQWT010000476         1334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_129026_length_1334_cov_2.249414, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000476 DAOQWT010000000
VERSION     DAOQWT010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129026_length_1334_cov_2.249414"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PKJ95_08005"
     CDS             <1..1068
                     /locus_tag="PKJ95_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNR66216.1"
                     /translation="NGAASMGDVRKLGKVGIKTIVFFLISTAIAIVIGLTMAFLINPG
                     IGLDMGAIGAVEYTARAGKPIAEVLYEMVPRNPIQALAEGNMLQIIVFAIVVGIALTL
                     IGEKGKKVLELLEQLNELVMKLVGIVMSLAPLGVFCLVARTFGQIGISVIVPLGKSVI
                     AVYIALAIQLIVVYGIMLKGFAGLSLTRYIKKLSPAMAIAFSTCSSAAALPANMDLTI
                     NRIGASKDISSFVLPLGNTVNMNGTAIYQGVAAVFIAQAYGIPVTPTMALMIVLSATL
                     ASIGTAGVAGAGAIMLSMVLHTVGLPIEGVGLIFGIDRIYDMGRTAMNITGDSVVAAI
                     ISKQEKEFDEKIWNTDFAKDE"
BASE COUNT          398 a          253 c          289 g          394 t
ORIGIN      
        1 aatggtgcag ccagtatggg agatgtcagg aaactgggaa aagttggtat taaaacaatc
       61 gtattcttct taataagtac agccatagct attgtaatcg gtcttaccat ggcattcctt
      121 atcaatccgg gcattgggtt ggatatgggt gccataggtg cggtggaata tacggcaaga
      181 gcagggaaac caatagcaga agttctctat gagatggtac ccagaaatcc gattcaggca
      241 ctggcagaag ggaatatgct tcagattatc gtatttgcta tcgtagttgg tattgctctg
      301 accctgatag gtgaaaaggg taaaaaggtt ctggaattat tggaacaatt gaatgaatta
      361 gttatgaaat tagttggaat cgttatgtct ctagctcccc tgggagtgtt ctgtctggta
      421 gccagaacct tcgggcagat tgggatttcg gttatagttc cattggggaa aagtgttatc
      481 gcggtataca tagctttagc tattcagtta atcgtcgtat atggtataat gctgaaagga
      541 tttgccgggt tgtcactgac cagatatatc aagaaacttt caccggcaat ggcaattgcc
      601 ttctccactt gtagtagtgc ggcggcattg ccggcgaata tggatttgac cataaacaga
      661 ataggtgctt ctaaagatat atcctcattc gttcttcccc tgggtaatac agtaaacatg
      721 aatggtactg ccatatatca gggggtagca gcggtattta ttgctcaggc ctatggaatt
      781 ccagtaactc ccaccatggc acttatgata gtactatcag ctacactcgc ctccatcggt
      841 accgcaggag tggcaggtgc cggtgcaata atgctctcaa tggttttaca taccgtagga
      901 cttcccattg agggtgtggg gttaatattt ggtatagaca gaatatacga tatgggcaga
      961 actgctatga atatcacagg agactctgtg gtagctgcca taatctccaa acaggaaaaa
     1021 gaatttgacg aaaaaatctg gaataccgat tttgccaaag acgagtagtt taatattaat
     1081 attttttaaa aattatagag agaggggctt ctgcccctct tttttctccc cctcttccac
     1141 ccccacatct atatttgaat ttgcatttcc accaaaaact ataaactatt aactaaaaac
     1201 ctccctcatt tatgcttaca atgtattaac taaaaacctt atgcaagcga ttgttttcca
     1261 agaaaatgtc taaaatcctt taaaataggg aatattaaat atataatcat tcaatgccat
     1321 actcaaatta ctca
//
LOCUS       DAOQWT010000477         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_129490_length_1331_cov_3.045455, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000477 DAOQWT010000000
VERSION     DAOQWT010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129490_length_1331_cov_3.045455"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PKJ95_08010"
     CDS             complement(<1..837)
                     /locus_tag="PKJ95_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNR66217.1"
                     /translation="MAMVARFVKEKGITGEFLLNLLISKKIISNKELEDICKRQGENN
                     FSIQRVILGTGIMSKKDMMQLLSDEIGVPYVDLEGKTFDPSIVAMLSEEISRKNNLVT
                     IGKKKDKLIVAMANPLDVYIHDEIRITTGYEIEPYLALSDDINKTLDSVYGITENLQQ
                     MIGEIASLPVTAVEEEAEDMEITKSVTESHEAPVIALVNLIMLRAIKEGASDIHIEPS
                     GDRQLKVRYRIDGILHDIMSPPKSLHMAIISRVKIMSNLNIAERRLPQDGRIKVQVHG
                     REI"
     gene            complement(1056..>1331)
                     /locus_tag="PKJ95_08015"
     CDS             complement(1056..>1331)
                     /locus_tag="PKJ95_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNR66218.1"
                     /translation="LYSRYKPRRKPFREAMKIMNTNNKETAVVGDQIFTDIWGGNRLQ
                     LLTILVCPLSRSDSLGTTIISRVLEGFLLSILFRNGKIELTKGKWPN"
BASE COUNT          369 a          288 c          229 g          445 t
ORIGIN      
        1 tatctccctg ccatggacct gcactttgat tcttccatcc tgcggcagac ggcgttcggc
       61 aatattgaga ttggacataa tcttgactcg tgaaataata gccatatgca gactcttggg
      121 aggcgacata atatcatgca gtatcccatc aattctatat cttaccttaa gctgacggtc
      181 accagagggc tctatatgga tgtcgctggc cccttctttg atagcccgga gcataatgag
      241 attaaccagt gctataacag gggcttcatg gctttcggta acgcttttgg tgatttccat
      301 atcttcagct tcttcttcca ctgcggttac cgggagtgag gcaatctcac ctatcatctg
      361 ctgtaaattt tcggtaatcc catatactga atccagagtt ttattaatat catcactgag
      421 agccagatat ggttctatct cgtatcctgt agtaattcta atctcatcat ggatataaac
      481 atccaggggg tttgccatcg ctacaataag tttatctttc tttttaccaa tggttaccag
      541 attattctta cgggaaatct cttcggaaag cattgctaca atagaggggt caaatgtctt
      601 tccctctaaa tccacatagg gtacgccaat ttcatccgag agcagctgca tcatatcttt
      661 tttgctcatt atccctgttc ccaaaattac tctctgaata gaaaaattgt tttccccctg
      721 tcttttgcag atatcttcta actctttgtt gctgataatt ttcttgctga taagcaggtt
      781 taagagaaat tctccggtta ttcctttttc ctttacaaaa cgggctacca ttgccatttt
      841 atccttactt tcagttttta taatattgag tattcaaaat gctgtttttt attttgctta
      901 tacaatcata tacaatgata tacaatgaaa caatatatta ttatacccct accagtaaaa
      961 aagagcaaca aatcattcca gcccggggga gagtatctat tatagataat aatgtgatat
     1021 gcccataaga ccataatact ataagaattg aaatattaat tgggccactt gcctttggtt
     1081 aactctattt taccatttct aaacaaaatt gataataaga atccttccag aacacgactg
     1141 atgatggtag ttcccagtga atcagagcga ctcaaagggc agaccagtat ggttaagagc
     1201 tgcaggcgat ttccacccca tatatcggta aatatctgat ctcccactac cgctgtttct
     1261 ttattgttgg tattcattat tttcatagct tccctgaagg gctttctcct gggcttataa
     1321 cgactatata a
//
LOCUS       DAOQWT010000478         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_129812_length_1329_cov_3.220565, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000478 DAOQWT010000000
VERSION     DAOQWT010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129812_length_1329_cov_3.220565"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /gene="istB"
                     /locus_tag="PKJ95_08020"
     CDS             complement(<1..780)
                     /gene="istB"
                     /locus_tag="PKJ95_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNR66219.1"
                     /translation="MEHLLKKLALPRIRLIYRDWIDRAGKEGMSYSDFLRGLLEEEVC
                     AREESRLRNLMKKANFPFEKTLEQFDFRVHPELRHQVFQNYLEESFVREGRALVLIGA
                     PGLGKTHISVAIGIKMVQRRFDVRFITVQRLVNQVLSAETAKEKNRILRTYRNCPLLI
                     LDEFGYLLPDSETGPVLYELIAERYEKRATIITSNKSLTEWGKIIQDNSLASALIDRL
                     MHHGDVYYFKGESYRMKGKERILEQQSKQQQKSASEEGKVNG"
     gene            complement(765..>1329)
                     /locus_tag="PKJ95_08025"
     CDS             complement(765..>1329)
                     /locus_tag="PKJ95_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66220.1"
                     /translation="KESLVMIDTNRYSVPAIYVGQVLTGRIREKVIEFYDSSKCVAKH
                     QRRLHQSKPVIDPAHYETVFEKKPRARVMVYRDFLMAQDKSISAFIAELCRRHKGTYG
                     KQILEMHNMWQKHGTDELGVACALASEHGAYGSDYLSSLLRTPDRGQLIVSLNLKEEL
                     PKQNEIDRALSQYENYAIGGVSSWNTF"
BASE COUNT          350 a          310 c          204 g          465 t
ORIGIN      
        1 accattaact ttaccttcct ctgaggctga cttttgctgt tgttttgatt gttgttctaa
       61 aattctttct ttccctttca ttctgtaact ttctcccttg aagtaatata catcaccgtg
      121 atgcatcaac ctgtcaatca gagcactggc aagagagtta tcctgaataa ttttacccca
      181 ctcagtaaga ctcttgttac tggtgattat ggtagctctt ttttcatacc tctctgcaat
      241 gagttcatat aggacagggc ctgtctctga atctgggagc agataaccaa attcatctaa
      301 aatcaataga ggacaattgc ggtaagtacg tagaattcta ttcttttcct ttgctgtctc
      361 tgcagaaaga acctggttta ccaatctctg cactgtgata aatcgaacgt caaatctacg
      421 ttgaaccatt ttaatcccta tagctactga aatgtgcgtc ttcccgagac cgggagcccc
      481 tattaatacc agagccctcc cttctcttac aaaactctct tcaagataat tctgaaatac
      541 ctgatgacgt agttcaggat gaactcgaaa atcaaattgt tccagtgtct tttcaaaagg
      601 gaaattcgct tttttcataa gatttctcaa cctgctctct tctctggcac atacttcttc
      661 ttccagaaga cctcttaaaa aatcactgta actcattcct tctttccctg cacgatctat
      721 ccagtcacga tagataagtc ttattcttgg taaagcaagc ttttttagaa ggtgttccaa
      781 gaactaacac ctcctatggc ataattttca tactggctta aagccctgtc tatttcattc
      841 tgttttggca gctcttcttt tagattaaga cttactatta attgacctct atccggtgtt
      901 ctaagcaaac tgctcaaata atctgagcca taggcaccat gttccgatgc cagagcacag
      961 gctacaccta actcgtcagt tccatgcttc tgccacatat tatgcatctc taatatctgc
     1021 tttccatatg tccccttatg gcggcggcag agttctgcta taaatgcact tatactttta
     1081 tcctgggcca tcaagaaatc tctgtatacc attactcttg ctcttggctt cttttcaaat
     1141 actgtttcat aatgagcagg gtctattaca ggttttgatt gatgtaaccg tctttgatgt
     1201 ttggcaacac attttgaact atcataaaat tctattactt tctctcttat tcttcctgtt
     1261 aatacctgac ctacataaat tgcaggaact gaatatctat ttgtatctat cattacaagg
     1321 ctttcttta
//
LOCUS       DAOQWT010000479         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_131104_length_1322_cov_2.139700, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000479 DAOQWT010000000
VERSION     DAOQWT010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131104_length_1322_cov_2.139700"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..>1322)
                     /locus_tag="PKJ95_08030"
     CDS             complement(29..>1322)
                     /locus_tag="PKJ95_08030"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HNR66221.1"
                     /translation="RPPYHPGDLLRVYLYGYLNHIRSSRRLETETRRNVELMWLLRKL
                     TPDFKTIADFRKDNKRALVRVFRDFTQLCSNWQLFGKELLAIDGSKFRASNSKRNNYS
                     QKKLVRHINYLNEKIAGYLAELDEADQAEDKDRRPTAGEIKERIDQLKARKAKYQGYQ
                     DILNETGKNELSTIDPDARLMNNNHRVEVSYNVQTSVDAKHNLIADFKVTDSPNDQRQ
                     LAPMVLRSKKILGSNGFTVLADKGYYHVHDLVYLDKRKITTYVPEKTYANSTGDRQFF
                     PDQFKLDTESNCYYCPAGERLSYYQSKREYGKVVGYEYRNQKACQSCPVKTRCSKNKK
                     GRTIFRHKDQDILDGIARKTQENLSTYKLRQMMVEHPFGTIKRGWGASYFLTRRKASV
                     NAEMALSLLAYNLKRVINILGTEEILRRLRENSQPVPV"
BASE COUNT          306 a          303 c          302 g          411 t
ORIGIN      
        1 agaaaagaaa aaagggaata ccggcctcct ataccggtac tggctggcta ttttctctta
       61 atcttcttaa gatttcctct gttcctaaga tattgattac ccgctttaga ttataggcca
      121 atagtgataa ggccatctcg gcatttaccg aggcctttct cctggtcaga aaataggagg
      181 caccccagcc ccttttgatg gtgccgaagg gatgctctac catcatctgc ctgagcttat
      241 aggtggaaag gttctcctgg gtctttctgg caataccatc taagatgtcc tggtctttat
      301 gacggaagat ggttcggccc tttttgtttt tactacagcg ggttttaacc gggcagctct
      361 ggcaggcctt ttggttacgg tattcataac cgaccacttt accatattct cttttgctct
      421 ggtaatagga taacctctca ccggctggac agtagtaaca attactctca gtatcaagct
      481 tgaattgatc cgggaaaaat tgcctatctc cggtagaatt ggcataggtc ttctcaggga
      541 cataggtagt gatcttcctc ttatctagat agactaaatc atgaacatgg taatacccct
      601 tatcagccag tacagtaaag ccattgctac ccagtatctt tttactccgt aagaccatgg
      661 gggctaactg tctttgatca ttggggctat ctgtcacctt aaaatcagca atgagattat
      721 gcttggcatc tactgaggtt tgcacattat agcttacttc cacccggtgg ttgttattca
      781 tcagccgggc atccgggtca atggtggaaa gttcgttttt acctgtttca tttaagatgt
      841 cctgatatcc ctggtatttg gcctttcttg ccttcagctg gtctattctc tctttaatct
      901 caccagctgt aggccttcgg tccttatctt cagcctggtc agcctcatcc agttctgcta
      961 gatatccggc aatcttttca ttgaggtagt tgatatgacg taccagtttc ttctgggagt
     1021 agttgttgcg cttactgttg gaggcacgga acttggaacc gtcaatggcc agaagctcct
     1081 tgccgaagag ctgccagttg ctgcacagtt gagtgaaatc acggaagacc ctgactaagg
     1141 ctctcttgtt atccttgcgg aaatcagcaa tagtcttaaa atcaggagtg agttttctta
     1201 ataaccacat gagctctaca ttacgtctgg tctctgtctc taagcgtctg gaggagcgga
     1261 tatggttcag gtaaccgtag aggtagactc tcaagagatc cccggggtgg tagggtggtc
     1321 tg
//
LOCUS       DAOQWT010000480         1320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_131422_length_1320_cov_2.562846, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000480 DAOQWT010000000
VERSION     DAOQWT010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131422_length_1320_cov_2.562846"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PKJ95_08035"
     CDS             complement(<1..747)
                     /locus_tag="PKJ95_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR66222.1"
                     /translation="MKEIGKYLKNRRIELGISLEEAELALKIRKKYLAAIEEGEESVL
                     PGKTYFTGYLRNYANYLQIDQDYINQLLDNSEQDSKPIEIEPPIEKKKTRRYFSQKKR
                     KMPVKKEKKPINVIPLLKAVLTILLIVGFIFVLNQFIYKLRQPSTSILEKDSITQEKT
                     IEQEMIEIAKEKIEQEEIPEDHDLPFLNPLPDYNPIELIASEPSWVKIIQDEQVLFEG
                     IITAEEKIIVKSNGLVSLITTSPNRINVTYN"
     gene            complement(915..>1320)
                     /locus_tag="PKJ95_08040"
     CDS             complement(915..>1320)
                     /locus_tag="PKJ95_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR66223.1"
                     /translation="SVGEYPQAADTLGVNVTKMRYIAVLISGGLAGLAGSVLSIEQAH
                     FFVKEMSGGRGFIALAAMIFGKWTPFGTAAAALLFGIGEALAIRVQGVYTIPVQFINM
                     IPYLLAILVLVGAIGRATAPATSGIPYEKEIS"
BASE COUNT          393 a          246 c          201 g          480 t
ORIGIN      
        1 attataagtc acattgatcc tgttcggact ggtagtaatt aatgaaacta acccattcga
       61 tttcactata attttttctt cagccgtaat aatgccttca aataaaactt gttcatcctg
      121 aattattttt acccagctcg gctcactggc aataagttca attgggttat aatcaggtaa
      181 tggatttaaa aaaggtaaat cgtggtcttc agggatttct tcctgttcaa tcttttcctt
      241 agctatctca atcatttcct gctctattgt tttttcttgt gtaatgctat ctttttcaag
      301 tatagatgtg gaaggttgcc gtagtttata tataaattga ttaaggacga aaataaatcc
      361 aacaattaaa agtattgtca agacggcttt taataaaggg attacattga ttggtttttt
      421 ttctttcttt acaggcattt ttcttttctt ctgagaaaaa taccttcttg ttttcttttt
      481 ttctattggt ggctcaatct caattggttt agaatcctgc tctgaattat ctaaaagttg
      541 attgatatag tcctggtcta tttgaagata attggcataa ttccttaaat aaccagtaaa
      601 atatgttttt ccgggaagaa cgctttcttc tccttcttct attgctgcta aatatttttt
      661 tctaatttta agggctaact ctgcttcctc taaggaaatc cccaattcaa ttcttctatt
      721 tttcaagtat ttccctattt ctttcatttt ataaatatct cctatggtta tttattacca
      781 gatattttga agattaaggt attataggct tttttaatat ttttagttaa agaattttaa
      841 tattttcaac ctttttctaa atgataacac aaaagcaccg aaatgctacc ataatgctgt
      901 aactaaatag ttaatcaaga tatttctttt tcatacggaa ttccactggt tgcaggagca
      961 gtagccctgc ctatagcccc aactaaaaca agtattgcca ggagataagg aatcatatta
     1021 ataaattgga cagggatggt ataaactcct tgtactctta tggctaaagc ctctccgata
     1081 ccgaataaga gggcagctgc agctgttcca aaaggtgtcc atttaccaaa tatcatagct
     1141 gccaaagcaa tgaaacctct gcctccgctc atctctttta caaaaaaatg tgcctgttca
     1201 atagagagaa ccgatcctgc taaaccagct aaaccaccag agattaatac cgctatatat
     1261 ctcatctttg taacattaac acccagggta tcagcggctt gagggtattc gccaactgaa
//
LOCUS       DAOQWT010000481         1319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_131595_length_1319_cov_2.481013, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000481 DAOQWT010000000
VERSION     DAOQWT010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131595_length_1319_cov_2.481013"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKJ95_08045"
     CDS             complement(<1..556)
                     /locus_tag="PKJ95_08045"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNR66224.1"
                     /translation="MAIILWFYISSEYNITSEKYFEIEVTPINLNEKLSIIEIREKIS
                     VGVKGPQNIIENLTPSKIIGTIDLQNILEAGEYILGVNIITPKNVEIMKIIPENIRVT
                     LEKIINVEYSVDYNLIGLPEKGYSLKEEPEIVPIEVLITAPESVHKIINQVKVDIDIS
                     GINKNISREEKIIVYYSDGKNEILP"
     gene            complement(620..>1319)
                     /gene="cdaA"
                     /locus_tag="PKJ95_08050"
     CDS             complement(620..>1319)
                     /gene="cdaA"
                     /locus_tag="PKJ95_08050"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNR66225.1"
                     /translation="GFLILFIFSFISNWIGLRTINWLIKGLMAMLVVAIPIIFQPELR
                     NILLKMGKGGFLTNFPLFPREEESKKWEAVINGIVMVAPMLSAEKIGSLIVLERDVGL
                     QDILETGISLNSDFSSELLYSIFLPNSPLHDGAVIIRNNRIAGANCILPLTKRTDIKK
                     SLGTRHRAAIGLSEISDALVIVISEEKGTISVAIDGRLTRPLRPELLKKFLQNLYRPK
                     RSIIIYNIPKKGKL"
BASE COUNT          422 a          236 c          170 g          491 t
ORIGIN      
        1 ctggcagtat ttcattttta ccatcagagt aataaacaat aattttttct tctctgctga
       61 tatttttatt aataccggaa atatctatat caacttttac ctgattaatt attttatgga
      121 ctgattcagg tgcagtaatc aaaacttcta taggaacaat ttccggttct tcttttaatg
      181 aataaccctt ttcaggtaaa ccaatcaaat tataatcgac tgaatattcc acatttataa
      241 ttttttccag ggttacacga atattttccg gaattatctt catgatttca acattctttg
      301 gtgtaatgat atttacacca agaatatatt caccggcctc taagatattt tgcagatcta
      361 ttgtaccgat tattttagaa ggagttaaat tctcaattat attctgcggc cctttgaccc
      421 caacactgat cttttcccta atctcaataa tagatagttt ctcatttaag ttaattgggg
      481 taacttcaat ttcaaaatat ttttctgatg tgatattata ttcactggaa atatagaacc
      541 ataatattat agccattaat aaagacaaga ttttaatatc gaaattgttt gttatccatt
      601 tttttattct ttccattttt tataatttgc cttttttagg aatattgtat attattatac
      661 ttctttttgg tctatataaa ttttgtaaaa acttttttag cagctctggt cttaaaggtc
      721 tggtcaatct tccatcaata gccactgata tggttccctt ttcttctgaa attacaataa
      781 ccaatgcatc cgatatctcg cttagtccaa tagcagcacg gtggcgagtt cccaatgatt
      841 tttttatatc tgtcctttta gttaagggca agatacaatt tgccccagca atacgattat
      901 ttcttataat tacagcccca tcatgaagag gagaatttgg taaaaaaatg gaatataaaa
      961 gttctgaaga aaaatcgcta tttaaagaaa tacctgtttc caatatgtct tgaaggccaa
     1021 catctctttc taacactatt aaagacccta ttttttccgc tgacaacatt ggtgccacca
     1081 ttactattcc atttataaca gcttcccatt ttttgctttc ctcttcccga ggaaaaagag
     1141 gaaaatttgt taaaaaacca cctttcccca tttttaataa tatatttcgt aattcaggct
     1201 ggaatataat gggaattgct accactaaca tagccattag gcctttgatt agccaattaa
     1261 tagttcttaa tcctatccag ttagagataa aagaaaaaat aaataagatt aaaaaaccc
//
LOCUS       DAOQWT010000482         1314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_132381_length_1314_cov_5.613185, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000482 DAOQWT010000000
VERSION     DAOQWT010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132381_length_1314_cov_5.613185"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..>1314)
                     /locus_tag="PKJ95_08055"
     CDS             complement(225..>1314)
                     /locus_tag="PKJ95_08055"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HNR66226.1"
                     /translation="KNIEKQEEEDANKEVKNKLFNGKVSQGFIEADGIHIPKQGGKNK
                     SMEVKVAICYSGRENRYKTGNSEQKQLSDKVVYGDICKSEEFIEKASEFFNYFCNLMS
                     VLYVLILGDGAPWIKNFLSVYSRGIYQLDRFHLLRKLRSHFSRKKDDYEEIKKLIEEN
                     RIDEVQVKLKEKQSFFEEKISEYELKGRLEDRKDEYQSYKKRINFYRRKIKKVKELII
                     YIETNKEGINGINKYKGIFKECDLIIGSGGIENQVKNTIARRMKGQGKCWKVEGACAM
                     VKILTSLSNGWYSFDDYLNIFSLNRKPLVMPKKLKEKAVNSKGKREAYRMEQKYKGHI
                     PCNTSSSSPIGYLKKEFCNVDYPDVLHI"
BASE COUNT          362 a          269 c          154 g          529 t
ORIGIN      
        1 gggtagtgta aaataaaagc tggtttattc atactttgta caaatattac cttttgcagt
       61 aggcgcagct tcgctgcgcc actatagttc tgacttatac atatatttgt tcccatagaa
      121 gctaatacct ccattgattt taaaattata aatcaataat tttctattta tctgtaaaag
      181 aatacctttt ctgttataag gtaaaaaaca ttgttactct tttactaaat atgtaataca
      241 tccggataat ctacattaca aaattctttt ttcaaatacc ctatagggct tgatgatgat
      301 gtattacagg gaatatgacc tttatatttt tgttccatcc ggtaagcttc tcttttacct
      361 ttagaattta cagctttttc tttcaatttt ttaggcatta ctaaaggttt tctattcaga
      421 gagaaaatat tcaggtaatc atcaaaacta taccagccat tagaaagaga agtaagtatt
      481 ttcaccatag cacaggctcc ctctactttc caacacttac cctgaccttt cattctcctg
      541 gcaatagtat tttttacttg attttctata cctccgctgc caataatcag atcgcattct
      601 ttaaagatgc ctttatattt gtttataccg ttaatacctt ctttattggt ttctatatag
      661 atgataagct ccttaacttt ctttattttt ctcctgtaaa aatttattcg cttcttgtag
      721 ctttgatatt cgtcttttct atcttccaat ctgcctttaa gctcatattc ggagattttt
      781 tcttcaaaaa atgactgttt ctcttttaac tttacctgta cttcatctat cctgttttct
      841 tctatcaatt tctttatttc ttcataatca tctttctttc tgctaaagtg gctacgcagc
      901 ttcctcagta aatgaaatct atctaattga tatataccac gggaatatac ggaaagaaag
      961 ttttttatcc aaggcgcacc atctcccagg attaatacat acaatacact cattaaatta
     1021 cagaaataat taaaaaattc actggctttc tctataaatt cttcactctt acaaatgtca
     1081 ccgtagacta ccttgtcact taactgtttc tgctcgctat tgccagtctt gtaacgattt
     1141 tctcttccgc tataacatat ggccaccttt acttccatac tcttattttt accgccctgt
     1201 ttggggatat gtattccgtc tgcctcgata aatccctgag atacttttcc attaaatagc
     1261 ttatttttta cttctttatt tgcatcttct tcttcctgtt tttctatatt ttta
//
LOCUS       DAOQWT010000483         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_132581_length_1313_cov_2.949921, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000483 DAOQWT010000000
VERSION     DAOQWT010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132581_length_1313_cov_2.949921"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PKJ95_08060"
     CDS             <1..720
                     /locus_tag="PKJ95_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66227.1"
                     /translation="VIIPTLNGNKVILDMGANVDCKPIHLLHFALMGASYAKYVLDVE
                     QPKVALLNIGEEMNKGNRLTREAYVLLKDREDLNFIGNIEGRDIFMGTADVMVCDGFI
                     GNIVLKTAEGLSTMFMTDFKLSILRNLPESQTTMLLKERFSDYASKRNYKTYGGAPLL
                     GVNGLCFICHGRSKAEAIKNALHSASLFAENKGLEHLKAIDFNSKDTSQDETQQEANQ
                     KNSCYNTGIFNHGDSFESDNN"
BASE COUNT          464 a          194 c          247 g          408 t
ORIGIN      
        1 gtaattatac caaccctaaa tggaaataaa gttattctcg atatgggagc aaatgttgat
       61 tgcaaaccaa tacacctact ccattttgct ctgatgggtg cttcttatgc taaatatgtg
      121 ttagatgtcg aacagccgaa agtagcctta cttaatattg gggaggagat gaataaagga
      181 aatcgtctga ccagagaggc atatgtttta ctcaaggata gagaagacct taatttcatt
      241 gggaacattg aaggcaggga tatctttatg ggtactgcgg atgtaatggt ctgtgatgga
      301 tttattggta atattgtctt aaaaactgca gaaggtctga gcaccatgtt tatgactgat
      361 tttaaactca gtattctaag aaatcttccc gagagccaaa ctaccatgct tttaaaagaa
      421 aggtttagtg attatgccag taaacgtaat tataaaacct atggaggagc accactatta
      481 ggagtaaatg gattgtgttt catctgtcac ggaaggtcca aggcggaggc tattaaaaat
      541 gcattacaca gtgcctccct gttcgcagaa aataaagggt tagaacactt aaaagcaatt
      601 gactttaatt caaaagacac gtcccaagat gaaacccaac aagaggccaa tcagaaaaat
      661 agttgttata acacgggcat ctttaaccat ggagattcct ttgaatcgga taacaactaa
      721 agtcacaaaa taggctacca cattcagagt gacaaaaata ggccaggtga ttatattgac
      781 aaccataggt ccaatataag gaatagattg aattaaagcc agtagataag taaaagtatt
      841 tgtgaatagg tcgactacta tgcccaccag agcaacaata acggttatca ggaaagcatc
      901 taaatgggta tatttaatga gatagtatat taatagaata ataagagcag ctaaaaggaa
      961 aaatcctgct gaagtttgag atttttttgg aattttaggt ttatattctt gactgaaagc
     1021 atctttttta ttgggttttt tgttgtccat taccgcctat tttttatttt aaaaaatttt
     1081 aattattttt ctaaaaaatt tagagcgctt actagttttt caaattgatc aatctaatat
     1141 ttattatttg ctatcatata aaaatttgct attcattgtc aaatctttaa attgggtaaa
     1201 ccatcttcta aacagagttt ttttaagaaa gacatgataa taaattagga tttttaagtg
     1261 tcaataactt tagaaaatgt cacctaaaag gggagttaat tatcgccaga taa
//
LOCUS       DAOQWT010000484         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_132706_length_1312_cov_15.210024, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000484 DAOQWT010000000
VERSION     DAOQWT010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132706_length_1312_cov_15.210024"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PKJ95_08065"
     CDS             complement(<1..827)
                     /locus_tag="PKJ95_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66228.1"
                     /translation="MAELTTWSVKMTDTLKEKISGSLQESGLTGKEFMEVLLNTYEQV
                     KKDRPEITPDLDELETLTKRICAIYVNVGERITTLLNDKDNSYKALVAEKTDLARTLQ
                     KRITELENSLKDKITIAKELAAEKDKIQEEKINLELRQQKEIQQLAEINQSNKSLIEE
                     YRQKNDTLTGLLAEYETYKNQIQELKKEVNLEKIKRAGAEDKFKKAEDTLNKNKEKLF
                     QTEERYKVQIESIIEQQSFEKEKALLEQEKSLRNELQAEREEYNNRIKTLLDEIEAR"
BASE COUNT          339 a          253 c          190 g          530 t
ORIGIN      
        1 ttccttgctt caatctcatc aagtaacgtc tttatcctgt tattatattc ttccctctct
       61 gcctgcagtt cattccttaa agatttctcc tgttccagga gggccttttc tttctcaaag
      121 gactgctgct ctattatgct ctctatctgc accttatatc tctcctctgt ctggaataat
      181 ttctctttgt ttttatttag tgtatcttct gccttcttga acttatcttc tgctcctgct
      241 cttttaatct tttccaggtt tacttccttc tttagctcct ggatctgatt tttatatgtc
      301 tcatactctg ccaggagtcc cgttaaagta tcatttttct gcctgtattc ctcaatcaaa
      361 gatttattac tctggtttat ttctgcaagc tgctggatct ctttctgctg ccggagctcc
      421 agattaatct tttcttcctg gattttatct ttttctgctg caagctcttt agcaatagtt
      481 atcttatctt taagagaatt ttcaagctct gttattctct tctgtaaggt gcgggctaaa
      541 tcggtttttt ctgcaacaag agctttatag gaattgtctt tatcattcag cagggttgtt
      601 attctttctc caacatttac gtatatagca cagattcttt ttgttagtgt ttccagttca
      661 tccagatccg gtgttatctc cggcctgtct tttttcacct gctcataggt atttaacagg
      721 acttccatga attctttgcc tgtaagcccg ctttcctgca ggctgcctga tattttctct
      781 ttcaaagtat ctgtcatttt aactgaccag gttgttagct ctgccatatt gataacctcc
      841 ttatgactta tgaatagttt actattttat taagtaaacc ttagtattta tagtattcta
      901 tagtttactt taaaatcctt ctttgtttta aaataattaa ttttttattt tattataaat
      961 gtcatataaa agctttattt ttattgtttt gccggtttgg atgtctgaaa tttcaccaga
     1021 gataagaaca attaaggatc gccaaaaaag tgaaaaatgt ttaattatat aggaatttta
     1081 tttgcaaaaa atgttataac gaaattcaag taaacccttg caattactag attattttga
     1141 tataattata agtataaagg aggtgtaaaa ttgaatataa gaataagttt aactcaacta
     1201 aaaaaatgaa agagggtata aagcaagaag agagttagtt ctaagtttcg cagctataaa
     1261 ctaacccatc ttcccggtaa atccattcta ccatgaaata cccttttcaa tc
//
LOCUS       DAOQWT010000485         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_132728_length_1312_cov_3.887033, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000485 DAOQWT010000000
VERSION     DAOQWT010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132728_length_1312_cov_3.887033"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..>1312
                     /locus_tag="PKJ95_08070"
     CDS             51..>1312
                     /locus_tag="PKJ95_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HNR66229.1"
                     /translation="MVKLLIRVDGSCIPNPGNMAIGVVIYRDGQLWKKINEIIGQGTN
                     NIAEYSAVIRGLEEIKDIPSHSVQFYCDSQLIVKQLNGQFKVKNKKMIPLYERIQELV
                     KEIKAPVFFIWNRREDNQMADQLARKLLLKEEQNKRALASQDLTVEDKGDHFLIQNKQ
                     NKKIYRVNLSLPECDCYDFENNCRKWNLECKHIVATRKFVEEREKQKSNDKDIVKKMK
                     VLIFSKMVKAEKWSELLEKYNRCDNLPLELIFPHPDSKELSRQLASVEVIVGGELGEF
                     ELNQAKKLKLFQIPFTGVDKQNLEVFKNYTDVTVCNTHGNSDAVSEQAFSLLLALAKN
                     LLNNDRDLRRGIWHGFVTGEPAIQVKGKNLAVIGLGTIGLEIAKRALAFGMKIYAIKQ
                     TIKKEEELEQKYGLTFLGTPEQLEYVIA"
BASE COUNT          480 a          182 c          292 g          358 t
ORIGIN      
        1 cttataaaaa agaaattgga agaaattagt cacaacaaaa agaggtgttt atggtaaaac
       61 ttttaatacg ggtggatggc tcctgtatac ctaatccggg gaatatggca ataggggttg
      121 ttatttaccg tgatggacag ttatggaaaa aaatcaatga aataattggt cagggcacca
      181 ataatattgc agaatatagt gctgtgataa gaggtttgga agaaataaaa gatattcctt
      241 cacattcagt tcagttttat tgtgatagtc aattaatcgt aaaacaatta aatggccagt
      301 ttaaggtaaa gaataaaaaa atgattccac tctatgagag gatacaagaa ttagtcaaag
      361 aaattaaagc ccctgtcttt tttatttgga acagaagaga agataatcag atggctgatc
      421 agctagccag aaaattgctg cttaaagagg agcagaataa aagagctctt gcctcccagg
      481 atttgacagt agaagataaa ggagaccatt ttttaataca aaataaacaa aataagaaaa
      541 tatatagagt aaacctttcc cttccggaat gcgattgcta tgattttgaa aataattgcc
      601 ggaaatggaa tttagaatgc aaacatattg tagctacccg gaaatttgtg gaggagagag
      661 aaaaacagaa atctaacgat aaagatattg ttaaaaaaat gaaagtattg atatttagta
      721 agatggtaaa agctgagaaa tggtcagaat tattggagaa atacaatcgg tgtgataact
      781 tgcccctgga gcttattttt ccccatcctg acagtaaaga attgagcaga cagttagcat
      841 ctgtagaggt aatagtggga ggagaactgg gcgagtttga gctaaatcag gccaaaaaat
      901 tgaaattgtt tcagatacct tttaccggag tggataaaca gaatctggaa gtatttaaga
      961 attatactga tgtgacagtt tgtaatactc acggcaatag cgatgcagtt tcggaacagg
     1021 ctttctctct tttattagcc ctggcaaaaa acctgctaaa caatgaccgg gacctgcggc
     1081 ggggcatatg gcatggattt gttaccggtg aaccggctat tcaggttaag ggaaaaaatc
     1141 tggctgttat tggtttgggt acgattggat tggaaattgc caaaagagcg ctggcttttg
     1201 gtatgaaaat atatgccatt aaacaaacca taaaaaaaga agaagaatta gagcagaaat
     1261 acggtctaac ctttttaggt acgcctgagc aattagaata tgttattgct ca
//
LOCUS       DAOQWT010000486         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_132735_length_1312_cov_3.624503, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000486 DAOQWT010000000
VERSION     DAOQWT010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132735_length_1312_cov_3.624503"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PKJ95_08075"
     CDS             <1..669
                     /locus_tag="PKJ95_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNR66230.1"
                     /translation="AGETIKNLSIDNRFTMCNMAIEAGAKSGIIEADEITEEYLKGRA
                     EKEYNIFKSDLDAEYEQVIEINVADIEPQVAFPHLPENCKGISEVKDILIDQSVIGSC
                     TNGRIEDMRIAAEILKNNKVNKRVRLIIIPATQQIYLEALEEGLLKIFIESGAIVSPP
                     TCGPCLGGHMGILAKGERAISTTNRNFVGRMGHPESEIYLASPAIAAASAITGKISSP
                     EEVI"
     gene            672..1181
                     /locus_tag="PKJ95_08080"
     CDS             672..1181
                     /locus_tag="PKJ95_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNR66231.1"
                     /translation="MIIKGKVWKFGDHIDTDIIIPARYLNTSDPNELALHCMEDYDKD
                     FIKNISPGDIIVAGDNFGCGSSREHAPIAIKEAGVSCVIAKSFARIFFRNAINIGLPI
                     FESSETSLGALQGDELEIFPEKGIIKNITQNELYNTNPMPIFIQNLIASGGLVEYTKK
                     EIERRQIEY"
     gene            1181..>1312
                     /locus_tag="PKJ95_08085"
     CDS             1181..>1312
                     /locus_tag="PKJ95_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNR66232.1"
                     /translation="MYNIALIPGDGIGPEVIREGKRIIDFASELNNVQINWVDFPFGA
                     "
BASE COUNT          469 a          200 c          281 g          362 t
ORIGIN      
        1 gcaggcgaaa cgattaaaaa cctttcgata gataatcgct ttactatgtg caatatggca
       61 attgaggcgg gagcaaaaag cggaatcatt gaagcagatg agataaccga agaatatctt
      121 aaaggaaggg cagaaaagga atataatatt tttaaaagcg atctggatgc tgaatatgag
      181 caggtaatag aaatcaatgt tgcagatata gaaccacagg ttgcttttcc tcatttacct
      241 gaaaattgta aaggtataag cgaagtaaaa gatatactaa ttgaccaatc tgtcatcggt
      301 tcctgtacca atggaagaat agaagatatg agaattgctg cagaaatatt aaaaaataat
      361 aaagtgaata aaagggtaag attaataatt atcccagcta ctcaacaaat atatttggaa
      421 gctttagaag aaggtttatt gaaaatattt attgaatccg gagccatagt tagcccacca
      481 acttgcggcc catgccttgg tggtcatatg ggtatactgg caaaaggaga aagagcaata
      541 tctaccacta atcgtaattt cgttggacgg atgggacatc ctgagagtga aatatatcta
      601 gccagtccgg caatagccgc tgcttctgcc attaccggga aaatatccag cccagaggag
      661 gttatttagc tatgataata aaggggaaag tgtggaaatt cggggatcat atagatacag
      721 atataatcat tccagcacgt tatctaaata catcagaccc taatgaattg gctttgcatt
      781 gtatggaaga ttatgataaa gattttataa aaaatatttc acccggtgac attattgttg
      841 caggggataa ctttggttgc ggaagctcca gggagcatgc accaatagct atcaaagagg
      901 caggtgtctc atgtgttatt gctaagtctt tcgcaagaat attttttcga aatgcaatca
      961 atataggttt acccatcttt gaatcatcag aaaccagttt aggagcctta cagggggatg
     1021 aactggaaat atttcctgaa aaaggtatta taaagaatat tacacaaaat gagctttata
     1081 ataccaatcc tatgcccatt tttatacaga acctaattgc ttctggtggg ttagtagagt
     1141 atacaaaaaa agaaattgag aggaggcaaa tagaatacta atgtacaata tagcattaat
     1201 tcctggagat ggtataggac cagaggtaat cagagaaggt aaaagaatta ttgattttgc
     1261 ttcagaacta aataatgttc agattaactg ggtagacttt cctttcggtg cc
//
LOCUS       DAOQWT010000487         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_133124_length_1310_cov_3.151394, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000487 DAOQWT010000000
VERSION     DAOQWT010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133124_length_1310_cov_3.151394"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /gene="proS"
                     /locus_tag="PKJ95_08090"
     CDS             complement(<1..1080)
                     /gene="proS"
                     /locus_tag="PKJ95_08090"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNR66233.1"
                     /translation="MDETKFVKEIASKEEAFSQWYIDVVKKADLADYTPVKGCMVIKP
                     YGYALWENMQQCLDRRIKATGHKNAYFPLFIPESLLKKEAEHVEGFAPEVAWVTHGGN
                     QKLEERLAVRPTSEAIIGSLYSKWIRSWRDLPVLINQWANIVRWEKVTRPFLRTTEFL
                     WQEGHTAHKNQEEAEEETLKILTVYKDYIEQDLAIPVIIGRKSNNEKFAGAMHTYTLE
                     ALMGDGKILQAGTSHNLGQNFAKAFDIRFLDENQQEQFAWTTSWGTSTRLVGGIIMVH
                     GDERGLKLPPKVASVQMVIVPIFFDTSKQEVMEKAREYKSLLEKKFKVELDDRDSYTP
                     GWKFNEWEMKGVPVRLEIGPRDLAQN"
BASE COUNT          352 a          281 c          222 g          455 t
ORIGIN      
        1 attctgagca agatcacggg gtccaatttc caatcgaacc ggaactccct tcatctccca
       61 ctcgttgaac ttccatcccg gagtataact atcccggtca tcgagctcca ccttgaactt
      121 tttttccagt aaggatttgt attctctggc tttctccatt acttcttgtt ttgaggtatc
      181 aaaaaagata ggaacaataa ccatttgcac tgaagcaact ttaggaggta attttaatcc
      241 cctttcatca ccatgaacca taattattcc gccaaccagt ctggtggaag ttccccagga
      301 ggtagtccag gcaaactgtt cctgctggtt ctcatccaga aaacgaatat caaaagcttt
      361 ggcaaaattc tgaccaagat tatgagaggt acccgcctgt aaaattttac catcacccat
      421 taaggcttcc aaggtatagg tatgcatcgc accggcaaat ttttcattat tactttttct
      481 cccgatgatt accggtatag cgaggtcctg ttctatataa tctttataaa ccgttaatat
      541 ttttaaggtc tcctcctctg cctcttcctg attcttatgg gcagtatggc cttcctgcca
      601 taagaattca gtggttctta aaaaggggcg tgttactttt tcccagcgta caatatttgc
      661 ccactggtta attaaaacag gcaaatcacg ccaggagcga atccatttag aatagagact
      721 gccgataata gcttctgaag tgggccttac tgccagcctt tcttccaact tttgatttcc
      781 cccatgggtt acccaggcta cttcaggggc aaatccttct acatgttcag cctctttttt
      841 taatagactc tctggtatga agagggggaa ataagcattc ttatggccgg ttgccttgat
      901 tcttcggtcc agacattgct gcatattttc ccacaaggca tatccgtaag gtttgataac
      961 catacatcct tttaccgggg tatagtctgc taaatctgct ttttttacca catcgatata
     1021 ccactgtgaa aaagcttctt cttttgatgc gatttccttt acaaattttg tttcatccat
     1081 aattacactc ctcagtaaat attagctata gtatttggta ttgtacaagt atttagcata
     1141 tagtatttag tatttgggtt ttattataat tttttattta gatattctta tatatttata
     1201 tatactcttt aaatgatgat attatattta gattaagtat tctttaatga gaaaattatt
     1261 agttttggct cccagcaatc tcagtttatc attaagagag tttactatgt
//
LOCUS       DAOQWT010000488         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_133738_length_1307_cov_2.060703, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000488 DAOQWT010000000
VERSION     DAOQWT010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133738_length_1307_cov_2.060703"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..678)
                     /locus_tag="PKJ95_08095"
     CDS             complement(421..678)
                     /locus_tag="PKJ95_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66234.1"
                     /translation="MDNYHKEVEKSKDSSKEGILLMIMAAFFFALMAFVIKLLDNIPL
                     IFAAILEITFLGVIPDPMSIIGSIMIIISGILHFKCSKNTG"
     gene            complement(722..>1307)
                     /locus_tag="PKJ95_08100"
     CDS             complement(722..>1307)
                     /locus_tag="PKJ95_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66235.1"
                     /translation="FNFLKGGKKNIYKDEIREYKNNILIFQKNGCLKQLSGHSLLSQK
                     TFSNLISIINDPEYDGVYIPGIIQETILKKIADKYYQLFQGKTIIINNPTFLLIGSRE
                     INHLKDLIGDLLNKGINIKTLYNLPILAVTINPFYPKYDRLRKGSYEMASIDRLQLHE
                     QMTGRVTFPVINIKEKESSKLLMEVKKFLKSGNR"
BASE COUNT          423 a          228 c          167 g          489 t
ORIGIN      
        1 cttcaaaggg ccctatccat atcggtaacc atttcttttc atcccggtct tttaaaatga
       61 ctacaaatcc tttggttgct ggatcaatcg ctaccccaaa aactttcacc tggatcatat
      121 ccttctcctc ctttcttctt aattaaataa cattagaata tcttaatttc tatttataat
      181 aattataaca tattatcttt cacataaaaa tcaatgttca tacagttaat gattttcatt
      241 agaaaacagc aaaaaataaa ttgtaaaaat tatagaataa gaagcttctg aggaaagaag
      301 gggttttaga aatagtttta cagcaattta aatatactca aacatttcta ttattgatat
      361 gactttggtt atttgaataa gttgacactg acctatttat ttcttgataa acagctgaaa
      421 ctaaccagtg tttttactac atttaaaatg taatatacca ctaatgataa tcataattga
      481 gccaatgatt gacattggat ccggtataac tccaagaaaa gttatttcca atattgcagc
      541 aaaaataaga ggaatattat ctaacaattt aataacaaat gccattagag caaagaaaaa
      601 ggctgccata atcattaaca atattccctc tttactgcta tctttactct tttccacctc
      661 tttatgatag ttatccatac tagtcctttt tgtttttact atatcggtta tattggtttt
      721 tttatctgtt accagacttt agaaatttct tgacttccat taacaattta ctactctctt
      781 tttccttgat gtttattacc gggaaggtta ccctgccagt catctgttca tgcaattgta
      841 atctgtcaat actggccatt tcataactac ccttcctcag cctgtcatac tttggataaa
      901 agggattaat ggtaaccgcc aaaataggta aattataaag tgtcttaatg ttgattcctt
      961 tattgagtaa atcgccaatc aaatctttaa gatgattaat ttctcgggaa ccaatcaata
     1021 gaaaagtggg attattgatt atgatagtct ttccttggaa taactgatag tatttgtcag
     1081 ctattttttt taaaatggtt tcttgtatga tacctggaat atatacccca tcatattccg
     1141 gatcattgat aatactgatc aaattgctaa atgttttttg actgagcaac gaatgtccac
     1201 tcaactgttt cagacatcca tttttctgga aaattagaat attattttta tactctctga
     1261 tttcgtcttt ataaatattt ttttttcccc ctttcaggaa gttaaat
//
LOCUS       DAOQWT010000489         1305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_133987_length_1305_cov_3.058400, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000489 DAOQWT010000000
VERSION     DAOQWT010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133987_length_1305_cov_3.058400"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKJ95_08105"
     CDS             <1..622
                     /locus_tag="PKJ95_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNR66236.1"
                     /translation="FYTLATFGLDLVTSINYSFVSRSTLIFSVILAYFFLNEGMNRGK
                     LFLIFLFVVGIYIISTEGKRIVLQFGDLLILLGTFCFSFYAIIQKILCKNLIPAIISW
                     AVTLSGVLIIILVSLVIRVGLLPENPYGFLYILFAGVGEALAVLFMNRTVHISNVTYY
                     TMMSMLTPVLNAFLGITFLNEPLTIIQLTGGVILIISGILVQRLKS"
     gene            complement(671..1252)
                     /locus_tag="PKJ95_08110"
     CDS             complement(671..1252)
                     /locus_tag="PKJ95_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66237.1"
                     /translation="MNKIDLQSSKYFRIELKRIQQKLGITSIYVTHNLKEAEEIADRI
                     AIMHQGKIEQIGTPQEIFFSPVNEKVSEFIGRPNIFSCDNLLDLGNGIARADCQGLSV
                     VIPHDDKKVCKIALFPQDIYLSETLPPGPSINRFKGNIVDLNISNEIVRMRVQVGSKT
                     LLSELPYHLFERMNLQKGKEVFLIFKLKNIKCL"
BASE COUNT          368 a          227 c          227 g          483 t
ORIGIN      
        1 attttacacc ctggctacct ttggacttga tctggttact tctattaatt atagctttgt
       61 ctctagaagt acccttattt ttagtgtcat tcttgcttac ttttttttaa atgaaggaat
      121 gaatagagga aaattatttt taatattttt gtttgttgta gggatttata ttatttctac
      181 agagggtaaa agaattgtac ttcaatttgg ggacctctta attcttcttg gtaccttttg
      241 cttttctttt tacgctataa tccaaaagat actttgcaaa aatctgatcc ctgcaataat
      301 ctcctgggct gttactttga gtggagtact aataataata ctggttagcc ttgtcataag
      361 agtagggtta cttcctgaaa atccttatgg ttttctctat atattatttg ccggtgtagg
      421 tgaggcactg gcggtacttt ttatgaacag gactgtgcat atttccaatg tcacctatta
      481 tactatgatg tctatgctaa ctccggtatt gaatgccttt ctgggaatta cttttctaaa
      541 tgagccacta actattattc agcttaccgg tggagttatc cttattatca gtggcattct
      601 ggtccagcga ttaaagagtt agttagtatt ataaaaagta ggttcttatc catttataat
      661 tacaatttag ctaaagacat tttatatttt ttaatttaaa gattaaaaat acttctttac
      721 ctttttgcag gttcatcctt tcaaatagat ggtagggtaa ttctgagagc aaagttttac
      781 tgccaacctg cactctcatc ctcactatct cattactgat attgaggtca acaatattcc
      841 ccttaaatct atttatactg gggccgggag gaagtgtttc agacagatat atatcctgtg
      901 gaaatagggc aattttacag accttcttat catcatgggg aatgactacc gataaaccct
      961 gacaatctgc tctggctata ccattaccta aatcgaggag attatcacag gaaaagatgt
     1021 tgggtctgcc aataaactca gatacctttt cattaaccgg agaaaagaat atttcttgtg
     1081 gtgttccaat ctgctctatc tttccctgat gcataatagc tattctatcg gcaatctctt
     1141 ctgcttcctt caggttatgt gttacatata tggaggtgat ccctaatttt tgttgtatcc
     1201 tttttaactc aattctaaaa tatttggatg actgtagatc aatcttgttc atggaaatgg
     1261 taagctaaaa tcccagtaag atggtaaaga gaaatcccag gataa
//
LOCUS       DAOQWT010000490         1305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_134000_length_1305_cov_2.812800, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000490 DAOQWT010000000
VERSION     DAOQWT010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134000_length_1305_cov_2.812800"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..>1305
                     /locus_tag="PKJ95_08115"
     CDS             52..>1305
                     /locus_tag="PKJ95_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNR66238.1"
                     /translation="MTYGLSLFPGGTETTVPHIRVGMDNSSGGIGWGYYDREKEQVIP
                     KYVTFKDLFSHIPRRGRASFIYGFPGLDNFNIYPLMNVPHLLKEYDVIQFFWFTTDAL
                     AHYFGERPYLESYKKFDKYLGKLVKNLDFDKENLIIYSDHGMSFGEFINIPQAKEIKR
                     IIGDGLKVYVHPHIYLKNSAEKNFWAKKIVLESEIEFAFFRENENSVIGYTDNGKILF
                     ERNDDGKIRYLNEGTDFFDYYKDGYQGEWLNADEWLSLTKKSNYPGVPTNIFNLFMNE
                     KSGDIVIVINPPKIPIFDLRYPANHAGLTKTDLLVPILLKGKALEHLYDVEDIWLHEL
                     FNEIPALNLSNNNPTRERQHFSFWGNYAEEKNYGFELLLSPAYRWNVALRYEQEIGKG
                     WFEYDVYSSYVIRLWAGAGIQYQFAD"
BASE COUNT          429 a          176 c          254 g          446 t
ORIGIN      
        1 atctattgat actttagtat cattaagaga gttcactatg tcttgatatt tatgacctat
       61 ggtttaagtt tatttcctgg tggaacagaa actacagttc ctcatattag agttgggatg
      121 gataattcga gtggaggaat tggctggggt tattatgacc gggaaaagga acaagtaatt
      181 cctaaatatg ttacttttaa agatctattt tcacatattc caagaagagg aagggctagt
      241 tttatttacg gatttcctgg attggataat tttaacattt atcctttaat gaatgtccct
      301 caccttttaa aagaatatga tgtaattcaa tttttctggt ttaccacaga tgctttagct
      361 cattattttg gtgaaaggcc ttatttggaa agctacaaaa aatttgataa atatcttggt
      421 aaattggtaa agaatcttga tttcgataaa gaaaatctaa ttatttacag tgatcacggt
      481 atgtcttttg gtgagtttat taatattcct caggcaaaag aaattaaaag aataattggt
      541 gatggtctta aagtatatgt tcatccccat atttatctga aaaattctgc agaaaaaaat
      601 ttctgggcta aaaaaattgt attagaaagc gaaatagagt ttgccttttt ccgggaaaat
      661 gaaaatagtg taattggtta tactgataat ggaaaaatat tatttgaaag gaatgacgat
      721 ggtaaaataa ggtatcttaa tgaaggaaca gatttttttg attactataa agatggttac
      781 cagggggagt ggctaaatgc tgatgaatgg ctatcgttaa ctaaaaaaag taattatccg
      841 ggtgttccta caaatatttt taatctcttt atgaatgaaa agtccggaga cattgttatt
      901 gttattaatc ctcccaagat acctatattt gacctacgtt acccggctaa tcatgccgga
      961 ttgaccaaaa ccgatttatt ggtacctatt ttactcaaag gtaaagccct ggaacatctt
     1021 tatgatgtag aagatatatg gttacacgaa ctgtttaatg agattcctgc tttaaacctg
     1081 tcaaataaca atccaactcg agaaagacag catttctctt tctggggtaa ttatgctgaa
     1141 gaaaaaaatt atggttttga actattatta tctcctgctt atcgttggaa tgtagcctta
     1201 cgatacgaac aggaaatcgg caaaggctgg tttgaatatg atgtatatag ctcttatgtg
     1261 attaggttat gggctggtgc gggaatacag tatcagtttg ccgat
//
LOCUS       DAOQWT010000491         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_134561_length_1302_cov_2.797113, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000491 DAOQWT010000000
VERSION     DAOQWT010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134561_length_1302_cov_2.797113"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKJ95_08120"
     CDS             <1..271
                     /locus_tag="PKJ95_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR66239.1"
                     /translation="ARYGTDPVLPNPVMAVDALLLVVDAIKRAGTTDGEALAKAIEDT
                     RNIKVLTTDNFSIDPATHNPFDKPAVINVVKDGKFEYVMSYSGES"
     gene            422..1294
                     /locus_tag="PKJ95_08125"
     CDS             422..1294
                     /locus_tag="PKJ95_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR66240.1"
                     /translation="MFLQTLVTGLGIGSIYALMAMGYCLVFSILNFSNFAHGAMIMIG
                     AYVGYLFCSIIGIPFFLSIIITAVITGILSVINERLAYRSLRERNAPSLYLIITAMGI
                     SIMLENLVYSTIGSNFYAYPRVFSKNYLEIFSSTIGLIDIYSFFVSIICIILLHLFLN
                     NSKIGMAIRAGVADPIATSLMGANFNVLIRNVFFLSGIFAGISGVFMGLRYMVYPQMG
                     WITNKAYIAAVIGGLGSLPGAIIGGFILGIIETIVSVYISSVVRDVFSFSMLILILLL
                     KPLGLFGIKYEEKM"
BASE COUNT          374 a          195 c          255 g          478 t
ORIGIN      
        1 agctcgatac ggcacagatc cagttcttcc caatcctgta atggctgttg atgcgttatt
       61 attagtagta gatgctataa agcgagcggg aactactgat ggtgaggctt tagccaaggc
      121 tattgaggac accaggaata taaaagttct taccaccgac aacttttcaa ttgatcctgc
      181 gacccacaac ccatttgaca agcctgcagt tattaatgtt gtgaaagatg ggaaatttga
      241 atatgttatg agctattcag gggaaagtta atatatcatt catgttaaga gattaacatt
      301 gttaattata taaattacag ctgtcagatt cttgttactc aattatattt ttagggtaga
      361 tgaatctgac agctgtaaaa atattccatt ggtaaaataa tcaggcagga ggggatttat
      421 attgttttta caaacactgg ttaccggatt aggtattggc agcatatatg ccttaatggc
      481 tatgggctat tgtctagttt tcagcatttt aaatttcagc aacttcgctc atggagcaat
      541 gattatgata ggtgcatatg tgggatatct gttctgctct ataattggga taccattttt
      601 tttatcaata atcattacag ctgttattac aggtatattg tcagttatca acgaaaggtt
      661 agcataccga tctttaagag aaagaaatgc gccatcactc tatttaatca ttactgcaat
      721 gggaatttct attatgcttg agaatttggt ctattctaca attggttcaa acttttatgc
      781 ctatccccgg gttttttcta aaaactatct tgaaatattt tctagtacca taggtttgat
      841 agacatttac tctttttttg tttctattat ctgtattatt cttttacacc tgtttctaaa
      901 taattcaaag attggaatgg caataagggc aggtgtcgcc gatccaattg ccacatctct
      961 aatgggggca aattttaatg ttttaataag aaatgttttc tttttgtcgg gcatatttgc
     1021 aggtatttcg ggagtattta tgggattgag atatatggtt tatccgcaga tggggtggat
     1081 taccaataaa gcttatattg ctgcggttat tggtggtctt ggtagtctgc caggtgccat
     1141 tattggtgga tttattcttg gaattattga aactatcgta tctgtttata tttcctcagt
     1201 agttagggat gtattttctt tttcaatgtt aattctaata cttttattaa aaccattagg
     1261 cctttttgga ataaaatatg aagaaaagat gtagcaatct tt
//
LOCUS       DAOQWT010000492         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_136185_length_1293_cov_2.600162, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000492 DAOQWT010000000
VERSION     DAOQWT010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136185_length_1293_cov_2.600162"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKJ95_08130"
     CDS             <1..428
                     /locus_tag="PKJ95_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNR66241.1"
                     /translation="RKKIIGLVHAGWKGTVSLISQKTVQLMQSHYGSRVQDIICAIGP
                     SIGPCCYEVGNEVIDKVKESFPEEKSLIFFDSKKGKYFFDLWKANNIQLLDCGIPDDN
                     IETASLCTSCHSDLFFSSRKEHQCTGRFAAGIMLKKLWK"
     gene            complement(680..>1293)
                     /locus_tag="PKJ95_08135"
     CDS             complement(680..>1293)
                     /locus_tag="PKJ95_08135"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNR66242.1"
                     /translation="LALTGDHPNMGDHPQATPVFDVDSIGLIKIIQELNKGIFSGNKS
                     SSYRTNFFTGIASNPGIGDMGKEVLRLEEKFAAGANFIQTQPVFSIKILEKYLDATRH
                     IPLPKIIGIMPLRSKKMVGYIEKNLAHIFIPENIKNRMSDSGIEEGINIARELIREYL
                     TNKEIAGIHIFPMNKLSIVPQLIMIDSADSGEQLHALNYSLSH"
BASE COUNT          405 a          210 c          249 g          429 t
ORIGIN      
        1 gcagaaaaaa gattattgga ctggttcatg ccggctggaa ggggactgtc agtctgatta
       61 gtcaaaaaac tgttcagctg atgcaatcac attatggttc cagagtacag gatattatct
      121 gtgctatagg tccttctatc ggtccctgtt gttatgaggt ggggaatgag gtaatagata
      181 aagtcaaaga atcttttccg gaagagaaaa gtttaatttt tttcgattct aaaaagggaa
      241 agtatttttt tgatctatgg aaagccaata atatacaact gttagattgt ggtatccccg
      301 atgacaatat cgaaacagcc agtctctgta cctcctgtca ttctgaccta tttttttcca
      361 gcagaaagga acatcaatgt accggccgtt ttgccgctgg aattatgtta aaaaaattat
      421 ggaaataagg caaacacttt ccctccccat ccggatttct atcaatattt tcgatgttga
      481 gaagttaata aacaaatggt agaggtagga ttcgaatctg ttttattgtt atgtgagttc
      541 ttaaaaaaag attctggaag aaggtaataa aaacaggaat atcaatattg taatctaatc
      601 tgaggattat tttatttaaa taaaattata aggaaaatga ggttgtaaca gaataggaaa
      661 aaattgttta gtaaactatt tagtgagaca atgaataatt taaagcgtgt aattgttcgc
      721 cagaatcagc tgaatctatc attataagtt ggggaacaat tgataattta ttcatgggaa
      781 agatatggat accggctatc tctttgttcg tcaggtattc tctaattagt tcccgggcaa
      841 tatttatgcc ttcttctata ccagaatcag acattctgtt ttttatgttt tctggaataa
      901 aaatatgtgc aagatttttt tcaatataac ctaccatctt tttacttctg aggggcatta
      961 ttcctattat ctttggtaaa ggaatatgtc tggtagcatc aagatatttt tctaagattt
     1021 taatgctgaa tacaggctgc gtctgtataa aattagctcc agcagcaaat ttctcttcta
     1081 acctgagcac ttcctttccc atatccccta ttcccgggtt acttgctatc cctgtgaaaa
     1141 agttagttct ataactcgaa cttttgttgc ctgaaaaaat gcccttattc agttcctgga
     1201 tgattttaat caaaccgata gaatcaacat caaaaacagg agttgcctga gggtggtcac
     1261 ccatattggg atggtctccg gtaagggcaa gta
//
LOCUS       DAOQWT010000493         1289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_136878_length_1289_cov_3.141005, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000493 DAOQWT010000000
VERSION     DAOQWT010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136878_length_1289_cov_3.141005"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="PKJ95_08140"
     CDS             complement(<1..981)
                     /locus_tag="PKJ95_08140"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNR66243.1"
                     /translation="MELNQNLKKVKPSGIRKLFDLAQNRKDVISFGIGEPDFITPAHV
                     REAAKQAIDDGYTHYTPNAGFYDLRKVLAEKLTEVNKIPVKVEQVIVTAGGTQALFSA
                     FYILLNPGVEIIVPDPGFLVYGSQVILAGGNPVYLPIREKNAFQIDPDELKSLITSKT
                     RAILINSPSNPTGMVIKDDVLKEIAKIAIEHNMLVISDELYEDILFDGKEHISIASFP
                     GMAERTISIFGFSKSYAMTGWRLAYLVCPEFLVNEIIKVQQNTAVCPNSVTQRAALYG
                     LQHKEETELEIEKMRKAYQERRDILVAGLNEIEGFHCLVPQGAFYAFPNIT"
     gene            complement(1030..>1289)
                     /locus_tag="PKJ95_08145"
     CDS             complement(1030..>1289)
                     /locus_tag="PKJ95_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNR66244.1"
                     /translation="NQIIERLGKKVNNLHLIDAERIAKEAGNIITANIVILGAALATP
                     QFPVKKDLIIESLKQNLPARSIDMNIKALEMGYQEYSNILN"
BASE COUNT          376 a          268 c          224 g          421 t
ORIGIN      
        1 ggtaatattg ggaaaagcat agaaggcacc ttggggaacc aggcagtgga aaccttcgat
       61 ctcatttaat ccggcaacaa gaatatcccg acgttcctga taggctttac gcatcttctc
      121 tatctcaagt tccgtctctt ccttatgctg taatccatat aaagcagctc tctgggtaac
      181 agagttggga caaactgccg tattctgttg tacttttatt atctcattta ctaaaaattc
      241 cggacagact agataggcaa gacgccagcc agtcatagca taacttttag aaaatccaaa
      301 aatagagata gttctttctg ccatacccgg gaaagaggca atactgatat gctccttacc
      361 atcaaacaaa atatcctcat agagttcatc gctgattacc aacatattat gctctatggc
      421 tattttggca atctccttca gtacatcatc cttgattacc ataccggttg gattgctggg
      481 agaatttata aggatagccc ttgttttaga ggtaattaaa ctctttaatt catcagggtc
      541 tatctgaaaa gcatttttct cccggatagg aaggtatacc ggattccccc ctgccagaat
      601 aacctgtgaa ccataaacca gaaaaccagg atcaggaaca attatttcaa ctcctggatt
      661 tagcaggata taaaacgcag aaaataaggc ctgagtgcct cctgctgtca ctataacctg
      721 ttcaacctta accggtattt tattgacttc tgtaagcttt tctgccagga cttttcgtaa
      781 atcataaaaa ccggcattgg gtgtataatg ggtataaccg tcatcaattg cctgcttggc
      841 agcttcacga acatgggcag gtgtaataaa atcaggttcg ccaattccga aacttatcac
      901 atcttttctg ttttgagcta aatcaaagag ctttcgaata ccagagggtt taaccttttt
      961 taaattttga tttaattcca tttttcttcc ttttacctct tttcttcatt aaagttaatt
     1021 tgtatttaat cagttaagaa tattagaata ttcttgatat cccatttcta aagcctttat
     1081 attcatatct atgctgcgag ccggtaaatt ttgctttaga gattcaatga tcagatcttt
     1141 tttgaccgga aattgaggtg tagcaagagc tgcccctaaa attactatat tggcagtaat
     1201 gatattacct gcttctttgg ctatcctttc agcatcaatg aggtgcaaat tatttacttt
     1261 tttgcccagt ctctcaataa tctgattta
//
LOCUS       DAOQWT010000494         1289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_136899_length_1289_cov_2.772285, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000494 DAOQWT010000000
VERSION     DAOQWT010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136899_length_1289_cov_2.772285"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PKJ95_08150"
     CDS             complement(<1..844)
                     /locus_tag="PKJ95_08150"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNR66245.1"
                     /translation="MICNKKGRHFMQGNIAVGEAAIISGARFFAGYPITPSSEIAEHA
                     SLNLPIAGGTFIQMEDEIGSLAAVIGASMAGVKAYTATSGPGLSLMNENIGLACMVEA
                     PCVIVSVQRAGPSTGLATKPAQGDFYQFRYGTHGDHMIIALSPASVQECFDLTIKAFN
                     FSERFRCPVFLALDAIIAKMYETVDIPGSDEIEILNRKIASTSDKDYLPYKPDENGVP
                     LFVPFGSSLITRANSSGHGIDGYPCADDKNHDRLVRRLIDKIDRYKEEIALFKEFNIS
                     DVEIL"
     gene            complement(854..>1289)
                     /locus_tag="PKJ95_08155"
     CDS             complement(854..>1289)
                     /locus_tag="PKJ95_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNR66246.1"
                     /translation="WQALGISQPKVAVLAAVEVVNPVMQATVDAACLAKMSNRNQIEH
                     FIIDGPLSYDTAMDPESAGGKGISNSLVAGDPDMLLVPNLETGNILGKSLKINSYAKS
                     AAIVFGADVPVMINSRSDSAERRYYSTLLARIILENKTKTKE"
BASE COUNT          388 a          293 c          230 g          378 t
ORIGIN      
        1 taaggatttc cacatcactg atgttaaatt ctttaaaaag agctatttct tctttatatc
       61 tatcaatctt atctattaaa cgacgtacca gcctatcatg atttttatca tcagcacagg
      121 gatagccatc tatcccgtga ccggaactgt tagctctggt aatcaaagaa ctgccaaagg
      181 gaacaaaaag aggtactcca ttttcatcag gtttataggg aagataatct ttatcagagg
      241 ttgacgctat ttttctattt aagatctcta tctcatctga acctggaata tcaactgttt
      301 cgtacatttt ggcaataatc gcatcaaggg caagaaaaac gggacagcgg aaacgttctg
      361 aaaaattaaa agctttgata gtcaaatcaa aacattcctg gacagaagcc ggggaaaggg
      421 caattatcat atggtcacca tgtgttccat aacgaaactg ataaaaatct ccctgggctg
      481 gtttagttgc cagaccggta cttggacctg ccctctgaac actaacaata acacaggggg
      541 cttccaccat acaggctaac ccaatatttt cattcatcaa tgacaagcca ggtccactgg
      601 tagcagtata tgctttaact ccagccatac tggcaccaat aaccgccgcc agactgccaa
      661 tttcatcctc catttgaata aaggttcctc cagctattgg cagattcaga gaggcatgtt
      721 ctgcaatctc agaagaaggg gtaataggat aaccggcaaa aaatcttgct ccagagatta
      781 tagctgcttc tcccactgca atattaccct gcataaaatg gcggcctttt ttattacaaa
      841 tcatttcttg ctttcactcc tttgtctttg tcttattttc taaaattatt ctggctaata
      901 atgttgaata atatcttctc tctgctgaat ctgaacgtga attaatcatt acaggaacat
      961 ctgccccaaa tacaattgcc gcagatttgg cataactatt gattttcaaa ctttttccca
     1021 aaatatttcc ggtttctaaa ttggggacaa ggagcatatc cggatcacca gcaactaatg
     1081 aattgctaat acctttacct ccggcggatt ccgggtccat tgctgtatca taggatagag
     1141 ggccatctat tataaaatgc tctatctgat tgcgattaga catttttgcc agacaggccg
     1201 catcaacagt ggcctgcata acaggattaa ccacctcaac tgctgctaaa acagctactt
     1261 tgggttgact tattcccaga gcctgccac
//
LOCUS       DAOQWT010000495         1283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_138031_length_1283_cov_2.312704, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000495 DAOQWT010000000
VERSION     DAOQWT010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138031_length_1283_cov_2.312704"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1157
                     /locus_tag="PKJ95_08160"
     CDS             3..1157
                     /locus_tag="PKJ95_08160"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNR66247.1"
                     /translation="MAEESLLAKGLVERIGIEGSLINHYPSGKDFFKKEIDIPDHRKG
                     IELVIEALLDSKYGVINSISEISAVGHRVVHGGEKYSGSVLLNEDVMNTLNEFIELAP
                     LHNPPNILGIKVCQDLLPDIPQVGVFDTAFHQSIPEQAFLYGIPYGFYEKYRIRRYGF
                     HGTSHKYVAQKAAEMLGKDLKELKIITCHLGNGSSITAVKEGKSIDTSLGFGTISGVI
                     MGTRCGDIDPAIIPYLMEKENLSFKEINNILYKKSGFMGLSEGISSDMRDLEEEAIKG
                     NKRAKRTLDVLYYGLKKYIGAYTAAMNGLDVLVFTAGIGENSPKLRRAVCEDLNFFGI
                     RIDDKINYDQNGKKAIISTEDSKVTVLVIPTDEEFMIAHDTAEICNNYRP"
BASE COUNT          451 a          169 c          288 g          375 t
ORIGIN      
        1 atatggcaga agaatccctg ctagccaaag gtttggtgga aagaattgga atcgaaggtt
       61 ccctgataaa tcactatcct tcagggaagg atttttttaa aaaagaaata gatatacctg
      121 atcaccgtaa aggaatagaa ctggtaatag aggctctgtt ggatagtaaa tatggtgtta
      181 taaatagcat ttcggaaata tctgcagtag gtcacagagt agttcatggt ggagaaaaat
      241 attctggttc ggtgttatta aatgaagatg taatgaatac tctcaatgag tttattgaat
      301 tggcaccact ccataatcca ccaaatatat tgggtatcaa agtgtgtcag gatttattgc
      361 cggatattcc tcaagtcggt gtctttgata cagctttcca tcaatcaatt ccggaacagg
      421 cgtttcttta tggtatacca tatggttttt atgaaaaata tcgaattaga aggtatggtt
      481 ttcatggaac ttcccataaa tatgtagccc aaaaagcagc agaaatgctg ggtaaagatt
      541 tgaaagaatt gaaaataata acctgccatc tgggtaatgg ttccagtata actgcagtga
      601 aggaaggaaa atcaattgat accagtctcg gttttggtac tatttccggt gtgataatgg
      661 gaacccgctg tggcgatatt gacccagcta ttattcccta tctgatggag aaagaaaatc
      721 ttagttttaa agaaattaat aatattctgt ataaaaagag tggctttatg ggtttatcag
      781 agggaatatc aagcgatatg cgggatttgg aagaggaggc aataaaagga aataaaaggg
      841 caaaaaggac tttggatgtc ttgtattatg gcttgaagaa atatatcgga gcatataccg
      901 cagcaatgaa tggtctggat gttctggtgt ttaccgctgg aattggtgaa aattctccta
      961 agttaagaag agcagtatgt gaagacttaa atttttttgg aattaggatt gatgataaga
     1021 taaattatga ccaaaatggt aaaaaagcaa taattagtac tgaagattca aaagtgacag
     1081 ttttagtaat accaactgat gaagaattta tgatagctca tgacacagca gagatttgta
     1141 ataattatag accataagcc ggtcattgat ttttgaaagg gtaatagatg tatcgaatta
     1201 ataggaagat aaaattatat caggatattt acagtatgtg gatagaagca cctgaaatag
     1261 caaaaacagt acagccggga cag
//
LOCUS       DAOQWT010000496         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_138145_length_1282_cov_3.348818, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000496 DAOQWT010000000
VERSION     DAOQWT010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138145_length_1282_cov_3.348818"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKJ95_08165"
     CDS             complement(<1..560)
                     /locus_tag="PKJ95_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HNR66248.1"
                     /translation="MKIALPRLGNIEVMNETIITDMGHELIEIPPNTKRTLDLGVRYA
                     PEYCCLPLKIVLGNFLEALELGAEGLIMAGGWGPCRFGYYAQVQRDILLDLGYDFKMI
                     IIEIPRGNWNMLWENLNILRNGRSWSYFINQFRLGWQKILLMEKLENMVLSVRPREKV
                     RGVSSRILKESMLKIKKAKNREMLKQ"
     gene            complement(532..>1282)
                     /locus_tag="PKJ95_08170"
     CDS             complement(532..>1282)
                     /locus_tag="PKJ95_08170"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HNR66249.1"
                     /translation="AFFCPKIIGLPDMVKSSLSSICPIISPVVDVRKSYGTWKNSLFQ
                     VGQLLTENTLAISRAISLSWENFQKNYVAHLLDPKVLRPASLIEGQDSINSKRQWEEK
                     PINIAVVGHDYLIDDSYINLNLIEKLKRMKVNVLTPGMVHPAMVKKQLKKLYKPIFWS
                     LSRKAVGAALYYFENGVEGIIHLTSFECGEDSMVGESIHHQSQKCPEVSYTEFVFDEH
                     TGEAGINTRIEAFLDMIRRKKRIENSLAPSW"
BASE COUNT          364 a          289 c          226 g          403 t
ORIGIN      
        1 atctgtttga gcatttcgcg atttttagct tttttaattt tcaacatact ctcttttaag
       61 atacgggaag agacacctct taccttttct ctgggacgaa ccgataagac catattctcc
      121 agtttctcca tcaataggat tttctgccag cccaatctga attgattgat aaaataagac
      181 cagcttctgc cattacgcaa gatatttaag ttttcccata acatattcca gtttccacga
      241 ggaatttcta taataatcat tttgaaatca tagcccaaat ctaacaggat atccctctgt
      301 acctgagcat aatatccaaa acgacagggt ccccaaccgc ctgccataat cagaccttca
      361 gcacccaact ccagtgcttc taagaaatta cccagtacga ttttgagggg caagcagcaa
      421 tactcaggtg catatctcac tcccaaatcg agcgttcttt tggtattggg cggtatctct
      481 atcaattcat gtcccatatc agtgatgata gtctcattca taacttcgat attaccaaga
      541 cggggcaagg ctattttcaa ttcttttttt cctccttatc atatcgagaa aggcttcaat
      601 acgggtattg atgccggctt ctccggtatg ttcgtcaaaa acaaattcag tataggagac
      661 ctccgggcat ttctggcttt gatggtgaat cgattcacct accatggaat cttctccaca
      721 ttcaaaagag gtcagatgaa taatcccttc taccccgttt tcgaaataat agagagctgc
      781 tcccactgct ttgcgggaga gactccagaa gattggtttg tacagctttt ttaactgttt
      841 ttttaccata gccggatgca ccattcccgg tgttaacaca ttcactttca tccttttcag
      901 tttttctatt aagttcaaat ttatatagga atcatcgatt aagtaatcat gacccactac
      961 agcaatattg ataggtttct cttcccattg tctcttactg tttatactat cctgcccttc
     1021 aataagagaa gcaggtctaa gcactttggg gtctaagaga tgtgccacat agtttttctg
     1081 aaaattctcc caggagagag aaatagctct agaaattgct aaagtgttct cagtaagcag
     1141 ttgccctact tgaaaaagac tgttcttcca ggtgccataa gattttctaa catccaccac
     1201 agggctgatt atggggcata ttgaactaag tgaggatttt accatatcag gtaagccaat
     1261 aattttggga cagaagaagg ca
//
LOCUS       DAOQWT010000497         1281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_138350_length_1281_cov_3.098695, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000497 DAOQWT010000000
VERSION     DAOQWT010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138350_length_1281_cov_3.098695"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PKJ95_08175"
     CDS             complement(<1..612)
                     /locus_tag="PKJ95_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNR66250.1"
                     /translation="MAKLKDLVNKPEILSPGHRLCAGCGASIAVRQILSASDDPVVVA
                     CATGCLEVATTIYPYTSWRSPFIHNAFENVAATISGVETMYQSLKKQGKIDKEIKFIA
                     FGGDGGTYDIGLQSLSGALERGHNFVYVCYDNEAYMNTGIQRSSATPRGAWTTTTPVG
                     EVIPGKTQYRKDLTRIIAAHNIPYAAQASIWRWNDLIDKAHKAF"
     gene            complement(626..>1281)
                     /gene="porA"
                     /locus_tag="PKJ95_08180"
     CDS             complement(626..>1281)
                     /gene="porA"
                     /locus_tag="PKJ95_08180"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HNR66251.1"
                     /translation="ERVDYLDDATVRNFIGTYQPNHPLLDIDKPLTFGPLDLYDYYFE
                     HKRQQAEDIKNSKPIILDVAREFKEISAREYGLFESYHLDDAETAIVVLSSAAGTAKA
                     VVDELRDRGRKVGLLKPRVFRPFPYIEIAQALSHLKAIAILDRSVSFGSFGGPLFTEI
                     RSAMVEFKKIPENFNIIFGLGGRDINEQDITGIYNQLDEVVKSGKVKERVQYLGVRD"
BASE COUNT          374 a          282 c          233 g          392 t
ORIGIN      
        1 aaaagcttta tgcgctttat ctattaggtc attccagcgc cagattgaag cctgcgccgc
       61 ataggggata ttatgtgctg caataatcct ggttaaatct ttccggtatt gtgttttacc
      121 tggaattact tctccaactg gtgtagtagt ggtccatgca ccacgaggtg tggcactgga
      181 acgttgaatt ccagtattca tatatgcttc attatcataa cagacataga caaaattatg
      241 acctctctct aaagctccgg aaagagattg taaacctata tcataggtgc cgccatctcc
      301 gccgaaggca ataaatttta tctctttatc aatctttccc tgctttttga gagactgata
      361 cattgtttca acaccactga tagtagctgc tacattttcg aaagcattat ggatgaacgg
      421 tgagcgccag ctggtatagg ggtaaatagt cgtagctact tccaggcaac cagtggcaca
      481 ggcaactact accgggtcat cactggcaga caatatctgc ctgacagcga tagatgcacc
      541 acatccggca cataaacgat gtcccgggct taaaatttcc ggtttattaa ctaaatcttt
      601 taattttgcc attttatttc acctcctaat cccttactcc aagatattgt actctctctt
      661 ttaccttacc tgatttaaca acttcatcta attgattgta gattccagta atatcctgct
      721 catttatatc ccgaccgcct aaaccaaaaa tgatattaaa gttttcaggt atttttttaa
      781 attcaaccat tgctgacctg atctctgtaa agagaggtcc tccaaaactg ccaaaggaaa
      841 cagaacggtc caggatggca atagctttca gatgtgataa ggcctgagca atttctatat
      901 aggggaaagg cctgaatact ctcggtttta aaagacctac ttttctaccc ctgtctctca
      961 attcatcaac aactgccttg gcagttccgg cagctgatga tagaacaaca atagcagttt
     1021 cagcatcatc aaggtgataa gattcaaata gaccgtattc cctggctgaa atttccttaa
     1081 attctctggc aacatccagt ataatcggct ttgaattttt tatatcttca gcctgctgac
     1141 gtttatgttc aaaataataa tcatagagat ctaatggacc aaaagttaat ggtttatcaa
     1201 tgtcgagtaa ggggtggttc ggctgatatg taccaataaa attacgcaca gtggcatcat
     1261 ccagataatc aactctttca a
//
LOCUS       DAOQWT010000498         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_138888_length_1278_cov_9.903516, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000498 DAOQWT010000000
VERSION     DAOQWT010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138888_length_1278_cov_9.903516"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1278)
                     /locus_tag="PKJ95_08185"
     CDS             complement(<1..>1278)
                     /locus_tag="PKJ95_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR66252.1"
                     /translation="YNYELQLSSNDPVSPLITVPLEYVVTTIAYIPDVNFRRAINEAL
                     GQPSDYQPTIADLNGLTGTLSAGERNIINIEGAQYLTNLQHLEFRWNQISDISPLAVL
                     TNLLSLDFGGNQISDLSPLAGLINLQWLHLWSNQISDLSPLAGMPNLQMLEICINNIS
                     DLSPLEDLTNLYYLNLSGNQISDLSPLANLTNLEVLSLYNNQISDLSPLSGLTNLKEL
                     WLGVNNINDLSPLEELTNLLELELSENQISDISPLEGLINLRKLWLDYNQISDLSPLE
                     GLTNLQYLWLNSNQISDLSPLAGLTNLLELYLNYNPISYESMLLSQTWPLPWSITSYN
                     SLSPCYPDPNRDAVNVSTGIDLSWQGNYDHSQDVFYEVWLGISPDSLFYQGLETQMVN
                     VTEHLYSFTPMLEPLTQYYWRIKAVSSTEEIWS"
BASE COUNT          421 a          249 c          250 g          358 t
ORIGIN      
        1 cactccagat ttcttcagtt gaagagacag cttttatccg ccaatagtat tgagttaagg
       61 gttccagcat tggagtgaag gaataaagat gttctgtaac atttaccatt tgagtttcca
      121 agccctgata gaaaagggaa tcaggactaa tgcccaacca gacctcatag aaaacatcct
      181 ggctatgatc ataattcccc tgccaggata aatcaatacc tgtagataca tttactgcat
      241 ctctattggg atcaggatag caaggcgaaa gggaattata acttgttatt gaccaaggta
      301 aaggccaggt ttgagataac agcattgatt cataagaaat aggattatag ttaagataaa
      361 gctctagcaa attagtcagc cccgctaaag gactgaggtc acttatctgg ttagaattaa
      421 gccacagata ttgcagatta gtcagaccct ctaaaggact gaggtcactt atctggttat
      481 aatcaagcca cagctttcgc agatttatca gaccctctaa aggactgatg tcacttatct
      541 ggttttcact aagctcaagc tctagcagat tagtcagctc ctctaaagga ctgaggtcat
      601 ttatattgtt aacaccaagc cacagttctt tcaaattagt cagacccgat aaaggactga
      661 ggtcacttat ctggttatta taaagagata gaacttccag attggtcaga tttgctaaag
      721 gactgaggtc acttatctgg ttaccactaa gattcagata atacagatta gttagatcct
      781 ctaaaggact gaggtcactt atattgttaa tacaaatctc cagcatttgc agattaggca
      841 taccagctaa aggactaagg tcacttatct ggttagacca aagatgcaac cattgcagat
      901 taatcagccc cgctaaagga ctgaggtcac ttatctggtt accaccaaaa tcaagagaca
      961 acagatttgt cagcaccgct aaaggactga tgtcacttat ctgattccaa cgaaattcca
     1021 gatgttgcag attagtcaga tattgagcac cttctatatt aattatattc cgttcaccag
     1081 cagataaagt tccagttaat ccattcaaat cagcaatagt aggttgataa tcagagggct
     1141 gacctaatgc ctcatttatt gcccttctaa aattgacatc aggaatatag gcaatagtag
     1201 taactacata ttctaaagga actgtgatca gaggtgaaac aggatcattg gatgagagct
     1261 gaagttcata attatagg
//
LOCUS       DAOQWT010000499         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_139867_length_1273_cov_3.394910, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000499 DAOQWT010000000
VERSION     DAOQWT010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139867_length_1273_cov_3.394910"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PKJ95_08190"
     CDS             complement(<1..207)
                     /locus_tag="PKJ95_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010651126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66253.1"
                     /translation="MMKKTKKLFLIDGQGLIYRAFFALPRLTTSHGQLINAAYGFTLV
                     LLRLLEEQKPDGIVIAFDTPKPTFR"
     gene            338..426
                     /locus_tag="PKJ95_08195"
     tRNA            338..426
                     /locus_tag="PKJ95_08195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:372..374,aa:Leu,seq:caa)
     gene            complement(430..>1273)
                     /gene="dnaE"
                     /locus_tag="PKJ95_08200"
     CDS             complement(430..>1273)
                     /gene="dnaE"
                     /locus_tag="PKJ95_08200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes DNA-template-directed extension of the
                     3'-end of a DNA strand by one nucleotide at a time.
                     Proposed to be responsible for the synthesis of the
                     lagging strand. In the low GC gram positive bacteria this
                     enzyme is less processive and more error prone than its
                     counterpart in other bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNR66254.1"
                     /translation="RAQLLSILEKSIQIAQDRQREKRNGQVSLFNIKSDSFVNNIEDT
                     FDWAGVKEFTDQELLLMEKETLGFYLSHHPLQDYQDKLLKITNADSTNLEKLSDKSQV
                     ILGGVITNMRKKATKNGNIMAYFSLEDLYGATEIIVFPKIMEESKEALQEENIVIVEG
                     RLDAMELNMKLLAESIAPINNYKKKSLPLSKKKNKNSSLLHIEIDITELELKKLKDLK
                     EILLKHPGREQVVMHFKSEHRIYHQYVDEQIKVEYSRKLEKEIKEYLPQARVWSEKED
                     YAEI"
BASE COUNT          361 a          250 c          211 g          451 t
ORIGIN      
        1 gcgaaaagta ggtttgggtg tgtcaaaagc aatcacgata ccatccggct tttgttcttc
       61 caataatctc agcaatacca gggtaaagcc ataggcggcg ttaataagct gcccatggga
      121 ggttgttaac ctgggtaagg caaagaaagc acgatatatt aaaccctgtc catcaattaa
      181 aaataatttt ttagtttttt tcatcatctt taaggttttt aatatttaga ataatcaata
      241 tcccgtaaaa aagaaaaaaa atgaaaggca tattagatag ctatttgctt gacattggta
      301 gtagtctggt ctataatttt tctgaaactt tcttaaagcc gaagtggcga aataggtaga
      361 cgcactggac tcaaaatcca gcgagcctca ccgctcatgt cggttcgact ccgaccttcg
      421 gcaccatttt taaatctctg cataatcttc cttttcagac cacaccctgg cctgaggcag
      481 atattctttt atttctttct ctaatttccg gctatattca acctttatct gctcatcaac
      541 atattgatgg tatattctgt gctctgattt aaaatgcatt accacctgtt cccttccagg
      601 gtgcttcagt aatatttctt ttaaatcctt tagcttttta agttcaagtt cagtgatatc
      661 tatttcaata tgcaggaggg aactattttt attttttttc ttacttaaag gtaaactttt
      721 tttcttataa ttattaatgg gagcaattga ttctgccagc aatttcatat tgagttccat
      781 agcatcaagg cgtccctcca caataactat attctcctcc tgtaatgcct ctttggactc
      841 ctccattatt tttgggaaaa cgatgatttc cgtagcgcca tagaggtcct ctaaagaaaa
      901 ataggccatt atattgccat ttttggtagc tttttttctc atattggtaa taacgccacc
      961 tagaataacc tgactcttat cagataattt ttctaagtta gtggaatcgg cattggtaat
     1021 ttttaataat ttgtcttgat aatcctgcaa agggtgatgg gaaagataaa aacccagggt
     1081 ttctttttcc attaaaagaa gttcctggtc ggtaaattct tttactcccg cccagtcaaa
     1141 agtatcttca atattattta caaatgaatc acttttaatg ttaaacagac ttacctgtcc
     1201 atttcttttt tctcgttgcc tgtcctgggc tatctgtatt gacttttcta aaatagatag
     1261 caattgagcc ctg
//
LOCUS       DAOQWT010000500         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_140062_length_1272_cov_2.916187, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000500 DAOQWT010000000
VERSION     DAOQWT010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140062_length_1272_cov_2.916187"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /gene="dnaE"
                     /locus_tag="PKJ95_08205"
     CDS             complement(<1..1202)
                     /gene="dnaE"
                     /locus_tag="PKJ95_08205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNR66255.1"
                     /translation="MNFTHLHLHSQYSLQDGLGSIDQIINQAQKLKMKSIALTDHDNM
                     TGAVEFYKKAIQAGIQPILGCEIRMRSKFFPGRLTHTVLLVKNSIGYRNLCQLVSQAH
                     LSNPQDIPSIEYELLFRYHEGLIALSGPLRGEVPLLIAQKEYQKAEEIASWFKNIFSP
                     ENFYLEISFHGLEQEKIINKYLIELGEKLSIPLVATNNVHYTEQEQAPFQSITNTIAN
                     QSGRECLHYPPLPNNQYYLKSGQEMKELFSFIPEALRNTEKIVQKCQFKLELGKIRLP
                     SYQPPQPYSASQYLEELCLEGLKKYYPDPSSQAFYRMKYELDVINQMGFAGYFLIVRD
                     IVNFAKQNNIPVGPGKGSSAGSLVSYLLNITEVDPLKFKLFFERFLNPQRIDLPDIDI
                     DFGQIGRD"
BASE COUNT          367 a          243 c          262 g          400 t
ORIGIN      
        1 ctatctctac ctatttggcc aaaatctata tcgatatccg gcaagtcaat acgctgagga
       61 ttgagaaacc gttcaaaaaa gagtttgaat tttaaagggt ctacctcggt aatgttgagc
      121 agataagata ccagactgcc ggctgatgag cctttgccag gtcccaccgg tatattgttt
      181 tgtttggcga aatttacaat gtccctgact atgagaaaat agccggcaaa gcccatctgg
      241 ttaataacgt ctaattcgta tttcatacgg tagaaagcct gactggaagg atcgggatag
      301 tattttttta aaccttccag gcataattct tctaaatatt gtgaagcaga atagggttga
      361 ggaggttggt aagagggtag ccgaattttc cccagctcca gtttgaactg gcatttttgg
      421 acaatttttt ccgtatttct caaagcttca ggaataaaag agaataattc tttcatctcc
      481 tgtcctgatt ttaaatagta ctggttgttg ggcaggggag gatagtgcaa acattcccga
      541 ccactctgat tggcaatagt attagttatg ctctggaagg gagcctgttc ctgttccgta
      601 tagtgtacat tattggttgc caccagaggt atagatagtt tttctcccag ttcgataaga
      661 tatttattga taatcttttc ctgttctaat ccgtgaaaag atatttccag gtaaaagttt
      721 tccggactaa agatattttt aaaccaggaa gcaatctctt ctgctttctg gtattctttt
      781 tgagcaataa gcagcgggac ctctcctctg aggggaccgg agagggcaat aagcccctca
      841 tggtagcgaa aaagcaattc atattcaatg gagggaatat cctgtggatt ggacagatgc
      901 gcctgactga ccagctggca gagattacga tatccaatac tattttttac cagaaggacg
      961 gtatgggtta aacgaccggg aaaaaattta ctcctcatcc ttatctcgca gccaagtatg
     1021 ggctgaatcc ctgcctgtat cgctttttta tagaattcaa cagcaccagt catattatca
     1081 tggtcggtaa gagctataga tttcatcttc aatttttgtg cctgatttat aatctggtca
     1141 atactaccca gtccatcctg taaactatat tgagaatgca gatgtaaatg agtaaaattc
     1201 ataagataat ccttctccta atccttttag caaacatatg tttgcttttc atatgaaaaa
     1261 aataattcta tt
//
LOCUS       DAOQWT010000501         1271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_140341_length_1271_cov_2.012336, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000501 DAOQWT010000000
VERSION     DAOQWT010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140341_length_1271_cov_2.012336"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKJ95_08210"
     CDS             complement(<1..582)
                     /locus_tag="PKJ95_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR66256.1"
                     /translation="MKDILQKFSLKGKTAVITGGAGILGSVIARGLGYGGARVLIADI
                     VDTKPLIDSMAEEGIEGKGYYLDVMNIDKIRACKDEIFKDFKQVDILINAAGGNKKEA
                     TTSEELSFFDLPLDALEKVIGLNLFGGAILPSQVFGKEMVKNENGGTIINISSMNAFR
                     PLTRIPGYSAAKAAVSNFTQWLAVHMCQNYSKNI"
     gene            complement(625..>1271)
                     /locus_tag="PKJ95_08215"
     CDS             complement(625..>1271)
                     /locus_tag="PKJ95_08215"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HNR66257.1"
                     /translation="GPTSPLAHLWVVSELQYQGVNFQNLAPHFIGDWEKGIEYIGDIN
                     TFKRELELHCQIVSHIGGYKLSLHSGSDKFSVYPIFARETGNLFHIKTAGTSWLEAAK
                     TISLCQPDLYREIHNFALTCFERDRFSYQLTTDLKRIPDIDRLKDEELVTLFSNNNAR
                     QLIHITYGSILREKDEEGRYRFRDRIFQTLFTHENTHYQAVSEHIKGHLDLLNF"
BASE COUNT          385 a          273 c          206 g          407 t
ORIGIN      
        1 gatgttcttg gagtaattct ggcacatatg cactgccagc cattgtgtaa aattactgac
       61 tgcagccttt gctgctgaat agcccggaat acgagtcaga ggacgaaagg cattcataga
      121 tgagatatta atgatggttc ccccattttc attttttacc atctctttgc caaatacctg
      181 agaagggaga attgctccac caaaaagatt aagaccgatt accttctcca gcgcatctaa
      241 tggtaaatca aagaatgata actcttcaga ggtagtagcc tcttttttat tacctccagc
      301 ggcattaatc aatatatcta cctgtttaaa atctttaaat atttcatctt tacaggctct
      361 tatcttatca atattcatta catctaaata gtatcctttg ccctctatac cctcttcagc
      421 catactatct atcagtggtt ttgtatcaac aatatctgcg atcaaaactc tggcaccgcc
      481 ataacctaat cctctggcta ttactgaacc aagtattccc gcaccaccag tgataactgc
      541 tgtttttcct tttaaactaa atttctgtaa tatatctttc atattattac ccctcctgca
      601 tttcttgaaa attattgtcc aaaactaaaa attcaataaa tccaaatgac ctttgatatg
      661 ttctgaaact gcctgataat gggtgttttc atgagtaaac agagtctgga atattcggtc
      721 tctaaatcta tatcgtcctt cttcatcctt ttctcttaaa atagaaccat aagtgatatg
      781 tatgagttgt ctggcattgt tattggaaaa tagggttacc agttcctcat cctttaacct
      841 atctatatcc ggtatcctct tcaaatcagt ggtgagttga taagaaaagc ggtctctctc
      901 aaaacaggtt aaagcaaagt tatgaatttc tctatataaa tccggctgac aaagggagat
      961 agtttttgct gcctccagcc aactggttcc agcagtctta atatggaaaa gattaccagt
     1021 ttcacgggca aaaattggat acactgaaaa tttgtcactc ccggaatgta atgataactt
     1081 atagccacct atgtgtgaga caatctggca atgcaattct aattctctct taaaagtatt
     1141 aatatctcca atatattcga tgcccttttc ccaatctcca ataaaatgag gagctaaatt
     1201 ttggaaatta accccctggt attgcaactc ggagacaacc caaagatgtg ccagaggcga
     1261 ggtgggacca g
//
LOCUS       DAOQWT010000502         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_140420_length_1270_cov_3.229630, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000502 DAOQWT010000000
VERSION     DAOQWT010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140420_length_1270_cov_3.229630"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKJ95_08220"
     CDS             complement(<1..403)
                     /locus_tag="PKJ95_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR66258.1"
                     /translation="MSDNGVILDVRSISKHFGALKAVDKVNFQVKKGEIVGLIGPNGA
                     GKTTITNLVTGELRKTEGEVHFKGINVSNMQPFQIARIDLTRTYQVVKPLQNMTVKEN
                     ILVGSLFGRSDRENDLNSARKTTHHIMELVGL"
     gene            complement(396..>1270)
                     /locus_tag="PKJ95_08225"
     CDS             complement(396..>1270)
                     /locus_tag="PKJ95_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR66259.1"
                     /translation="FTAIFMYATIAQCINLMSGYMGYLPFGHVMFFGIGAYISAILMK
                     TGLPVFVAIPAACFCAIVISIILGFPVLRLRGHYFAIATIGMNGAIMTVVQNATEITG
                     GARGTTFPIITVPPAVVYKYFYLAMFGLMVITSLVMNMIVNSKFGFGIRSIKANEDAA
                     NSMGINTTFTKVSTWAIAAMFTSIAGGLYGYWMSFIAPDEVFDLMFITNSIIMMLIGG
                     AGTILGPIFGAFIVETVSEVAWSGFMQYHLAALGIATIIIVFFIPRGLIGTITDKMRN
                     RRIKLSKKTEVTNV"
BASE COUNT          404 a          283 c          200 g          383 t
ORIGIN      
        1 ccaatcccac caattccata atatgatggg tagtttttct ggcactgttt aaatcatttt
       61 ccctatcact gcgaccaaaa agagagccaa ctaatatatt ctctttcaca gtcatattct
      121 gtaatggttt taccacctga tatgttctgg ttaggtcaat acgggcaatc tgaaatggtt
      181 gcatattgct cacattgata cccttaaaat gtacttctcc ttctgttttt cttaattcac
      241 cggtaaccag attagttatt gttgtttttc ccgcaccatt aggaccaatt aaaccgacaa
      301 tttctccctt ttttacctga aagttaacct tatccaccgc ttttagagcg ccaaaatgtt
      361 tgctaattga tctgacatct aaaataacgc cattatcaga cattggtcac ctccgttttt
      421 ttacttaatt taattctcct gttcctcatc ttatcggtta tcgtgccaat aagtcctctg
      481 ggaataaaaa atactattat tatagtagca atacctaaag ctgccagatg atactgcata
      541 aacccactcc aggctacctc tgataccgtt tctacgataa atgcgccaaa tatgggaccc
      601 aatatagtac cagcgccacc aataagcatc attattatag aatttgtgat aaacatcagg
      661 tcgaatacct catctggggc tataaaggac atccagtaac catacagacc accagcaatg
      721 ctggtaaaca tagcagctat agcccaggta gacacttttg taaaagtggt atttattccc
      781 atagaattag ctgcatcttc atttgcttta atgcttcgaa tcccgaaacc aaattttgaa
      841 ttgacaatca tattcattac caatgatgta attaccatca gtccaaacat tgccaggtaa
      901 aaatatttgt aaacaactgc aggcgggaca gtaataatag gaaaagttgt tcctctggct
      961 cccccggtaa tttcagtggc attctgcaca actgtcatta ttgctccatt cataccgata
     1021 gtggcgatgg caaaataatg tcctctcaat ctcagtactg gaaaacctaa aattatacta
     1081 atcacaatgg cacaaaaaca ggctgccgga attgctacaa aaacaggtaa cccggttttc
     1141 attaaaatgg cacttatata agctcctatg ccaaagaaca taacatggcc aaaaggtaga
     1201 tatcccatat agccagacat taaattgata cattgggcaa tggtggcata cataaaaatt
     1261 gctgtgaata
//
LOCUS       DAOQWT010000503         1269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_140592_length_1269_cov_3.592257, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000503 DAOQWT010000000
VERSION     DAOQWT010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140592_length_1269_cov_3.592257"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKJ95_08230"
     CDS             <1..628
                     /locus_tag="PKJ95_08230"
                     /inference="COORDINATES: protein motif:HMM:NF019940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HNR66260.1"
                     /translation="LLTSYLQSLYRLKEAARLEEDINLEAILRCREIFEFVKKEEDEG
                     KWESIKSSLNKALDLLVQSREEEGNRLMEDILKSVSRMVIIIKEIEKDKPENLKEFQE
                     KLIERIKQLTQNISLDEGRLETEVAFLAQKSDITEEITRLKSHISQLKRISKTVKPIG
                     KKLEFYTQEMGREINTMGAKSVGQSISQKVIKLKNELEKIKEQSRNIE"
     gene            643..903
                     /locus_tag="PKJ95_08235"
     CDS             643..903
                     /locus_tag="PKJ95_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNR66261.1"
                     /translation="MKRIMMNIGFGNYVNLERVLTVLDPESAPMKRLREEAKQSKMLV
                     NATYGRRTRSIIITDSNHIILSALQPETVVNRLTSYKKREDE"
     gene            921..>1269
                     /locus_tag="PKJ95_08240"
     CDS             921..>1269
                     /locus_tag="PKJ95_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR66262.1"
                     /translation="MVKEKGHLIVISGPSGVGKGTLIKIIFKNIPNLSYSVSVTTRKP
                     RKEEREGVDYFFVDEGTFKKYIEEHKFAEWAMVHGDYKGTLISTINETLTDGKDLILE
                     IDVQGALEIKNKYP"
BASE COUNT          493 a          166 c          294 g          316 t
ORIGIN      
        1 tcttttaaca tcctatctgc aatccttata ccggttaaaa gaggctgccc ggttggaaga
       61 ggatattaac ctggaggcaa tacttcgatg cagagagata tttgaattcg ttaaaaaaga
      121 ggaagatgaa gggaaatggg aatcgataaa atcatctctc aataaagctc tggatctatt
      181 ggtacaaagc agagaagaag aagggaacag gttaatggag gacattttaa agagcgtctc
      241 tcgtatggta ataataataa aggagataga aaaagacaag ccagaaaacc tgaaggaatt
      301 tcaggagaaa ttaatagaaa gaataaaaca actaacccag aatatttcac ttgatgaggg
      361 aagattggaa acggaagtag catttttggc acaaaaaagt gacattacag aagaaataac
      421 caggttgaag agccatatca gtcagttgaa acgaatctct aaaactgtga agcctattgg
      481 gaagaaatta gagttttata cccaggagat gggtagagaa ataaatacta tgggtgctaa
      541 gtcagtaggt cagagcatat cgcaaaaggt tattaagtta aaaaatgaat tagaaaagat
      601 taaagaacaa tcaagaaaca ttgaatagga taggagattt tgatgaaacg aattatgatg
      661 aacataggat ttggtaatta tgttaattta gaaagagtat taactgttct ggaccctgag
      721 tcggctccga tgaaaagatt gagagaagag gcaaagcaat caaaaatgct ggttaatgca
      781 acctatggca ggagaaccag gtctattatt ataacagata gtaatcatat tattctatct
      841 gccctacagc cggaaacagt tgttaatcga ctaaccagtt ataaaaagag agaggatgaa
      901 tagtaggcgg gtgaattaag atggtgaaag aaaagggtca tctgattgtt atttctggac
      961 cctcaggggt tggcaaaggg acgcttataa aaataatatt caaaaatatt cctaatttat
     1021 catactccgt ttcagtgact actagaaaac cgagaaagga agagcgtgaa ggagttgatt
     1081 atttctttgt agatgagggg acctttaaaa aatatattga ggaacataag tttgccgagt
     1141 gggcaatggt tcatggagat tacaagggta ccttaataag taccatcaat gaaacactta
     1201 cggatggaaa agatttaata ttggaaatag atgtacaggg agccctggag ataaaaaaca
     1261 aatatccgg
//
LOCUS       DAOQWT010000504         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_140790_length_1268_cov_3.384171, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000504 DAOQWT010000000
VERSION     DAOQWT010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140790_length_1268_cov_3.384171"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKJ95_08245"
     CDS             <1..741
                     /locus_tag="PKJ95_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR66263.1"
                     /translation="IWAALPGFLKAKKGIHEVIITIMMNWIATSLTFYLALLYKAPPT
                     EEMRLAGLPQMIPHTSEIAETARLPRIASLLSKINISFPSHNPLNVSFFIAVIAAILV
                     YYILWKTSLGYEIRAVGYNSAAAEYAGISIPKNILLAMMISGALAGLVGTNEIMGYKF
                     RWRQELFLGLGFNGIAVALLGKNHPLGVVLAAILFGTLSYGGALVNIFTAGKIPRELI
                     MVIQAVIVILVVISDEIIKRLILRRKTE"
     gene            741..>1268
                     /locus_tag="PKJ95_08250"
     CDS             741..>1268
                     /locus_tag="PKJ95_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR66264.1"
                     /translation="MFSSIINPDFFASAIRIAIPLALAGLGGVYSEKSGVVNIGLEGI
                     MLTTAFAAVAATNFSGNVWIGILIAAIVGMLLGLIHAIVTVSFKANQIVSGVAINLFA
                     SGITVFLSWLWFHETQIMALKSLPIWGFRVPAPEEASSLIRGLIVVFAHHSPMVYISF
                     IIIVISNILIFRTPFG"
BASE COUNT          340 a          223 c          278 g          427 t
ORIGIN      
        1 atctgggcag ccttgcctgg ttttttaaag gcaaagaagg gtatccatga agtaattata
       61 accattatga tgaactggat agctacttct ctgacttttt atttggcatt gttatacaag
      121 gctcctccaa ctgaagaaat gagactggca gggctaccgc agatgattcc tcataccagt
      181 gaaatagccg aaactgcacg gctgccaaga atcgcatcac tgttatccaa aataaatatt
      241 tctttcccta gccataatcc tttgaatgtt tctttcttta ttgctgttat tgcggctata
      301 ttagtctatt atattctttg gaaaacgagt ctgggttatg aaatcagagc agttgggtac
      361 aatagcgcag ctgctgaata tgccgggata agtataccga aaaacatctt gctggcaatg
      421 atgattagcg gtgccttagc gggtctggta ggaacaaatg agataatggg gtacaaattt
      481 cgctggcggc aagaactttt tcttggctta ggttttaatg gtattgcagt cgccttattg
      541 ggcaagaatc atcccctggg agtagtattg gcagccattt tattcggcac cctgtcttac
      601 ggcggtgcat tggttaatat ctttacggca ggcaaaattc cacgggaatt aataatggtt
      661 attcaggcag ttatagtaat tttggtggtc atttctgatg aaattattaa acggctaatt
      721 ctaaggagga aaacagaata atgttcagca gtattataaa tcctgatttt tttgcctctg
      781 cgataagaat tgccattccc ctggctttgg caggtttagg tggagtttat tctgaaaagt
      841 ctggagtagt taatataggg ttggaaggta ttatgctaac cactgccttc gcggctgttg
      901 ctgctactaa tttttcaggc aatgtatgga taggtatttt aatagctgcc atagtcggta
      961 tgttattagg tttaattcat gctattgtca cggtatcttt taaggctaac cagattgtaa
     1021 gtggggtcgc aattaacctt tttgccagtg gtattactgt ctttttaagc tggctctggt
     1081 ttcatgaaac acaaataatg gctttaaaaa gtcttccaat ctggggattc agagttcctg
     1141 ctccagaaga ggcctcatca ctgattagag gtttaatagt agtatttgct caccattcac
     1201 ccatggtata tatctccttt attattattg taatttctaa tattttaata ttcaggacac
     1261 cttttggt
//
LOCUS       DAOQWT010000505         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_141164_length_1266_cov_5.639141, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000505 DAOQWT010000000
VERSION     DAOQWT010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141164_length_1266_cov_5.639141"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>747)
                     /locus_tag="PKJ95_08255"
     CDS             complement(<1..>747)
                     /locus_tag="PKJ95_08255"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR66265.1"
                     /translation="GLTNLQNLDLGWNQISNLSPLAGLTNLQNLDLGWNQISNLSLLA
                     NLTNLQVLYLHFNQISDISPLAGLFDMKSLGLLSNQISDLSPLAGLINMEWLDLRSNQ
                     ISDLNPLAGLTNVQYLWLDANQINCISHLAGLTNLQYLYLENNQISDLSPLAGLTNLQ
                     DLDFRYNKISDLSPLVNLTNLLGLDLRDNPISYESMLLSQSWSLPWHISTYNPLSPCY
                     PDPSRNATNVLVNYPLSWQGNYDNSQDVFY"
     assembly_gap    748..847
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<848..>1266)
                     /locus_tag="PKJ95_08260"
     CDS             complement(<848..>1266)
                     /locus_tag="PKJ95_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR66266.1"
                     /translation="EYQPTIADLNGLTGTLSANVRNIISIEGAQYLTHLQYLELGSNQ
                     ISDLSPLAGLTNLQYLYLGGNQISDLSPLEGLTNLQELYLQNNRISDPIPLVELTNLL
                     SLGLGCNQIGDISPLAGLTNLNYLSLGENQISNLSPL"
BASE COUNT          388 a          224 c          225 g          329 t
ORIGIN      
        1 catagaaaac atcctggcta ttatcataat tcccctgcca ggataaagga taatttacca
       61 aaacattggt cgcatttcta ctgggatcag gataacaagg cgaaagggga ttgtaagttg
      121 aaatatgcca aggtaaagac caactttgag acagcagcat tgattcgtaa gaaataggat
      181 tatcacgaag atccagtcct agaagattag tcagattcac taaaggactg aggtcactta
      241 tcttgttata acggaaatcc agatcttgta gattagtcaa acccgctaaa ggactgaggt
      301 cacttatctg gttattttca agataaagat attgaagatt agtcaaaccc gctaaatgac
      361 tgatgcaatt tatctggtta gcatcaagcc aaagatattg aacattagtc aaccccgcta
      421 aaggattaag atcacttatc tggttagaac gaagatccag ccattccata ttaatcagtc
      481 ctgctaatgg actgaggtca cttatctggt ttgacaaaag tcccagagat ttcatatcaa
      541 aaagacccgc taaaggactg atgtcactta tttggttaaa atgaagatac agaacttgaa
      601 gattagttag atttgctaaa agacttaggt tacttatctg gttccaacca agatcaagat
      661 tttgcaaatt agtcagaccc gctaatggac tgaggttact tatctggttc caaccaagat
      721 caagattttg caaattagtc agacccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnncta atggactgag gttacttatc tggttttcac caagacttag ataattcaga
      901 ttagtcagcc ccgctaaagg actgatgtca cctatttggt tacaaccaag accaagagac
      961 aacagatttg tcagctccac taaaggaatg gggtcactta tccggttatt ttgaagatac
     1021 agctcttgca gattagtcag accctctaaa ggactgaggt cacttatctg gttaccacca
     1081 agatacagat attgtaaatt agtcagcccc gctaaaggac tgaggtcact tatctggtta
     1141 gaaccaagct ccagatattg cagatgagtt agatattgag caccttctat actaattata
     1201 ttccttacat tagcagataa agttccggtt aatccattca aatcagcaat agtaggttga
     1261 tattca
//
LOCUS       DAOQWT010000506         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_141240_length_1266_cov_2.493807, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000506 DAOQWT010000000
VERSION     DAOQWT010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141240_length_1266_cov_2.493807"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..>1266)
                     /locus_tag="PKJ95_08265"
     CDS             complement(98..>1266)
                     /locus_tag="PKJ95_08265"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNR66267.1"
                     /translation="IEGLPFLEAIRQFRNDIGKENVLYIHLSFFPYLKAAKEIKSKPT
                     QHSIKELRSIGIQPDLLICRAQAKLTESVKDKISLFCDIPKDGIIEMRDVESIYEVPL
                     VLEEQGLGNLIVRMLQLSTEKPDLKEWREMVNRLYHPERKASIAVIGKYVRFKDAYLS
                     INEALTHAGIDMKCKIETKLLDADLLLKNDLKNLLKGIQGILIHGGIGRKGIEGIVKI
                     IKFAREEKIPFLGICLGMECALIEFARNELKLVKANSVEFDPDTPYPIFDLLERLEEV
                     NIKDESLRIGSYSCNLTKGSIAYKIYQRDRIEERHRHSYGFNNRYLSKFEQSDIIFSG
                     VNLENNLVEIFELKNHPWFIGTQFHPEFKSRPNRPHPLFKSFLEAAIKMDSSEI"
BASE COUNT          357 a          265 c          196 g          448 t
ORIGIN      
        1 ttggtacagt tttctaattt cccggtatcc tttttcccag ccaggattaa tgcattcatt
       61 tcaaactaac tccaccttta attgaggtct gtttccatta tatctcagat gaatccattt
      121 ttatggcagc ttcgagaaaa cttttaaata atgggtgtgg acgatttggt cttgatttaa
      181 attcgggatg aaattgagtt ccaataaacc aagggtgatt ttttaattca aaaatttcaa
      241 caagattatt ttctaaattt actccactga atattatatc agattgctca aactttgaca
      301 aatagcggtt gttaaatcca tagctatgtc tatgtctttc ttcaatcctg tctctctgat
      361 aaattttata ggcaattgac ccttttgtca aattacagga atagctgcca atacgcagtg
      421 attcatcctt aatattcacc tcttccaatc tttccagtaa gtcaaaaatt ggatatggcg
      481 tatcggggtc aaattccaca ctatttgcct taaccagctt taattcattt ctggcaaact
      541 ctatcagggc acattccata ccgagacaga tacccaggaa gggtatcttt tcctcccggg
      601 caaattttat tattttgaca atcccctcta ttccttttct cccaatacca ccatgaatca
      661 atattccctg aattcctttt aatagatttt tcaaatcatt tttcaaaagc aaatcagcat
      721 cgagcagctt tgtttcaatt ttacatttca tatcaatgcc ggcatgagtc agggcttcgt
      781 taatactaag gtaggcgtct ttaaaacgaa catatttacc gataacagca atggatgctt
      841 tcctctccgg atggtatagt ctattcacca tttcccgcca ttcttttaga tccggttttt
      901 ctgttgataa ttgtagcatt cgtactatca aattacccaa accctgctcc tccagtacaa
      961 gaggtacttc gtaaattgat tctacatctc gcatttcaat aatgccatct ttgggtatgt
     1021 cacagaataa agatatttta tctttcacag attcagtcaa ttttgcctgt gctcgacaga
     1081 ttaacaaatc aggctgtatt ccaatactgc gtaattcctt tatactgtgt tgagtcggtt
     1141 ttgattttat ctccttggct gccttcaaat aggggaaaaa agaaaggtgt atgtagagaa
     1201 cattttcttt accaatatcg ttcctgaact gtctgattgc ttccaggaag ggtaatcctt
     1261 caatat
//
LOCUS       DAOQWT010000507         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_141791_length_1263_cov_2.847682, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000507 DAOQWT010000000
VERSION     DAOQWT010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141791_length_1263_cov_2.847682"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKJ95_08270"
     CDS             <1..182
                     /locus_tag="PKJ95_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNR66268.1"
                     /translation="NSIIAAGTVVTPRTIIPANSMVMGVPGKVVRKITEDEASYLKEH
                     AEGYVQLMKEYKEML"
     gene            322..>1263
                     /locus_tag="PKJ95_08275"
     CDS             322..>1263
                     /locus_tag="PKJ95_08275"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNR66269.1"
                     /translation="MARKSIADLIYSILEENHRPMHYRKITEEIKKIKEIKAENPHHA
                     INAVMGADNRFVRYQRGIWGLIKWKYREANLPYTLTTYCLLNGTIFLTSYLKPYFSWH
                     LEDRIIEVLFIDSEGEEIKAILNYRQKLITGFKEWFQKRKLKVNDTVLIGLIDQTRKR
                     YFIIAENEIRYDKEKKDMGETIYQILHNEGEPLSFTQIYSEITKQEPEYSSLFQEYIE
                     DILKNDARFILLHREKWALLEWLKEEEQLYHSLYFADSPESFQNSLQKCFEFLGYEVE
                     YDKETVQQLLIAHAHLASKSYSLIIAGLPGNYDLNTVR"
BASE COUNT          465 a          192 c          250 g          356 t
ORIGIN      
        1 aaaattcaat cattgccgcc gggacggtag ttactccaag aactataatc ccggccaact
       61 ctatggttat gggtgtaccg ggaaaagtgg taagaaaaat tactgaggat gaggcatctt
      121 atttaaagga gcatgccgaa ggttacgtac aattgatgaa agaatataaa gagatgttat
      181 aaaaacaaaa ataaatgaga ttcttaacaa aattcttttt ttacataagt caaagattaa
      241 ctaaagtcag caaaataaag tatcataaat agaattgata tttaaaagat agatgattgt
      301 attcccggtg ggaggtgaaa aatggccagg aaatcaatcg ctgatttaat ctatagtatt
      361 cttgaagaaa atcatcgccc catgcattac cgcaaaatta ctgaagaaat aaagaaaatt
      421 aaggagataa aagcagaaaa cccacaccat gcgattaatg ctgtgatggg tgccgataat
      481 agatttgtca ggtatcaacg cggaatctgg ggtttgataa aatggaagta tcgggaagct
      541 aaccttccct atactttaac tacttactgc cttctcaatg gcaccatctt tttgaccagt
      601 tatttaaaac cttatttttc ctggcacctt gaagaccgta taatagaggt gctctttatt
      661 gacagtgagg gagaagaaat aaaggcaata ctgaactacc gtcagaaatt aataaccggt
      721 tttaaagaat ggtttcaaaa aagaaaatta aaagtaaatg atactgtatt gataggttta
      781 attgaccaga ccagaaaaag atattttatt atagcagaga atgagatcag atatgataaa
      841 gaaaaaaaag atatggggga aacaatttat caaatcctgc ataatgaagg ggaacccctc
      901 agtttcaccc aaatttattc agaaattacc aaacaggagc ctgaatacag cagtttgttt
      961 caggaataca tagaggatat cttaaaaaat gatgctcgtt ttatcttgct gcacagggaa
     1021 aagtgggccc tgttggaatg gttgaaagaa gaggagcagt tataccacag tctatatttt
     1081 gcagatagtc cagaaagttt tcaaaactct ctgcaaaagt gttttgaatt tttagggtat
     1141 gaagtagaat atgataaaga aacagttcag caattactta ttgctcatgc ccatcttgcc
     1201 tctaaatcgt attctttaat tattgccggg ttaccgggaa attatgattt gaatactgta
     1261 cgt
//
LOCUS       DAOQWT010000508         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_141843_length_1263_cov_2.292219, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000508 DAOQWT010000000
VERSION     DAOQWT010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141843_length_1263_cov_2.292219"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PKJ95_08280"
     CDS             <1..744
                     /locus_tag="PKJ95_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNR66270.1"
                     /translation="IRGLFKVKSRLLYKINSMKNITIASLPDNFGVETDQGIAGVRIV
                     PLTIREKSIERIEQLCLREGTVFSVHKYQNLRVGIVTTGNEVYKGLVEDKFGPLLRKK
                     FSYFGAKVEEQIFCPDEKQKIVEAIKYFSEQSIELIALSGGMSVDPDDLTPGAIKESG
                     AKIITYGVPVQPGNMFMLANLGKSVLIGIPGASLFYKNTILDIVLPRIFAGETFKKQD
                     FIKMGEGGFCSNCSVCHYPHCYFGKRLLF"
     gene            777..>1263
                     /locus_tag="PKJ95_08285"
     CDS             777..>1263
                     /locus_tag="PKJ95_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66271.1"
                     /translation="MNFYREIKERFSRLIPDEALWSSTVNVVNVRPLTPQEVIGKPDR
                     DDFPLLKGKEVMIQADFKGSLGQAFTDMPGNFKGSLREVFQLPLASNFERAVFISTIN
                     AVLRYLGYIKDTVHCRNQEPAKCASLIKDYILEKYGIQKIAFIGLQPAMVETLATAFP
                     IR"
BASE COUNT          410 a          197 c          277 g          379 t
ORIGIN      
        1 atcaggggat tatttaaagt aaaaagtagg ctgctttaca aaattaattc tatgaaaaat
       61 atcactattg ccagccttcc tgataatttt ggagtggaaa cagaccaggg gatagcaggt
      121 gtacgcattg ttcccctaac aattcgggaa aagagtattg aaagaataga acaactctgc
      181 ctgagagaag gcactgtttt ttcagtacat aaataccaga atttaagggt aggaattgtt
      241 actaccggta atgaagttta taaaggattg gttgaagata aatttggacc actcctaaga
      301 aaaaaattct cttattttgg ggcaaaggta gaagaacaga ttttctgtcc agatgaaaaa
      361 cagaaaattg tggaggccat aaaatatttt tcagagcaga gtatagaact cattgcctta
      421 agtggaggta tgtcggtaga cccagatgac ctgactcccg gtgctattaa agagagtgga
      481 gcaaagatta taacttatgg agttccggta caaccgggga acatgtttat gctggctaat
      541 ctgggaaagt cagtattaat aggaatacca ggggcctctc tattttataa aaatactatt
      601 ttagatattg tcttacccag gatatttgcc ggggaaactt tcaaaaagca agattttatc
      661 aaaatgggcg agggtggttt ctgttccaac tgctctgtct gccattatcc acattgctat
      721 tttggaaaaa gattattgtt ttagaaagaa attttacagg aaagggaaat gcttaaatga
      781 atttttatcg agaaattaaa gaaagattta gtcgcttaat tccagatgag gccctctggt
      841 cttcaactgt taatgtggta aatgtgagac cacttactcc tcaggaggtt ataggtaaac
      901 ccgataggga cgattttcct ctattaaagg ggaaagaagt gatgatacag gctgatttta
      961 aaggaagttt ggggcaggct tttaccgata tgcccggcaa tttcaagggc agtttaagag
     1021 aagtctttca gttaccgctt gccagtaatt ttgaaagggc tgtttttatc tccactatta
     1081 atgctgtttt gcgttattta ggatatataa aagatactgt tcattgcaga aaccaggagc
     1141 ctgctaaatg tgcttctcta attaaggatt atattttaga aaaatatggt atacaaaaaa
     1201 ttgcctttat aggattacaa ccggccatgg tagaaactct ggccactgcc ttccctatta
     1261 gag
//
LOCUS       DAOQWT010000509         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_141976_length_1262_cov_3.307374, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000509 DAOQWT010000000
VERSION     DAOQWT010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141976_length_1262_cov_3.307374"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..>1262)
                     /locus_tag="PKJ95_08290"
     CDS             complement(38..>1262)
                     /locus_tag="PKJ95_08290"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNR66272.1"
                     /translation="FSERQAQAILDMRLQRLTSLETKKLEEEYLELIKRIAYLQDILE
                     NERKLMMCIKEELLVIKDKYADNRLTQIVKEEKELELEDFIAEEDIVITYTKDGYLKR
                     LPLNTYRKQRRGGRGVIGMGVKEEDLVDNLYITTNLHDILFFSSNGTVYRRRAYQIPE
                     GGRTSRGIAVVNLLGIQKGERITTVIPIREIEFEKSREQNDLFLYMATRQGKVKKVPL
                     HSFANLKNKEIIAISLNEGDELIRVRLAAENDNVILSTKKGKSAYFSGELIRSMGRTA
                     TGCRGIALGKDDHLIDISLVQPDSDEDLLLITENGSGKRTPVKSYRLAKTRGGKGVKS
                     INLSKERGNVISVKVVSERDELVIISQKGIIIRVSVKDISRTGRSSMGVKVMNLDKDD
                     KVASVANVSHTQIIE"
BASE COUNT          341 a          283 c          207 g          431 t
ORIGIN      
        1 taaatccaac catatcaatt cagaaatttt ataaaaatta ttcaatgatt tgggtatggg
       61 aaacattggc tacgctggct accttgtcat ccttatccag attcattacc ttaacaccca
      121 tagaactcct gccagttcta cttatatctt taacagaaac gcggataatg atcccttttt
      181 gacttattat gaccagctca tctcgctcac ttaccacttt aaccgatatg acattgcctc
      241 tttctttact gagatttatt gattttactc ccttaccacc tctggtttta gctagccggt
      301 atgacttaac tggtgttctt ttacccgaac cattttcagt aataagcagc aggtcttcat
      361 ctgaatctgg ctggaccaaa ctaatatcaa ttaaatgatc atctttgccc agagcaattc
      421 cacggcatcc tgtagcagtt ctacccattg aacgaatcag ttcacctgaa aaatatgctg
      481 atttgccctt tttggtagat agaataacat tatcgttttc tgccgctaac cttactctta
      541 tcaattcatc tccttcgttt aaagaaatag caataatttc tttatttttt aaattagcga
      601 aagaatggag aggaactttt tttacttttc cctgtctggt agccatataa agaaaaagat
      661 cattctgctc gcgggatttt tcgaattcaa tttcgcgaat gggaataact gtagtgatcc
      721 tctcgccttt ttgaataccc aggagattaa ccacggcaat gccgcgagaa gttctgcctc
      781 cctctggaat ctggtaggcc cttctccggt aaacagtgcc attacttgag aagaatagaa
      841 tatcgtgtaa attagtagta atatagagat tgtcaaccag gtcttcttct ttgacaccca
      901 taccgattac tcccctgccg cctctgcgct gtttgcgata ggtattcagg ggcagtcttt
      961 tcagataacc atctttggta taggtaataa cgatatcctc ttcagcaata aagtcttcca
     1021 gttccagctc tttttcttct ttgacaatct gggtaagtct gttgtcagca tacttgtctt
     1081 ttataactaa cagctcttcc ttaatgcaca tcataagttt tctttcattt tccaggatat
     1141 cctgtaaata agctattctt ttaataagtt caagatactc ttcttccagc tttttagtct
     1201 ccagagaggt gagtctttgt aaacgcatat ccagaatagc ctgggcttgg cgttccgaaa
     1261 at
//
LOCUS       DAOQWT010000510         1260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_142357_length_1260_cov_2.810788, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000510 DAOQWT010000000
VERSION     DAOQWT010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142357_length_1260_cov_2.810788"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..731
                     /locus_tag="PKJ95_08295"
     CDS             90..731
                     /locus_tag="PKJ95_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR66273.1"
                     /translation="MLGSILPYLVIILIFVGAMHTAVDITAGEKERGTLATLLVSQLS
                     RLEIVSGKYFSVMVISAISMLLGMVGLSIAFLVPAYLLGEVSIINVHFSFTLFLLFLL
                     ILAPLVGFASAILILIGIFARNSREASSYTTPIYMVAIFLGMISLSQGIELAQPLFFI
                     PILNNSFVFKELLMGTIDWNHITATLISNIGIALLALFGTVKLFNRESVIFRS"
     gene            876..>1260
                     /locus_tag="PKJ95_08300"
     CDS             876..>1260
                     /locus_tag="PKJ95_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR66274.1"
                     /translation="MKKADVVILGGLSGISAGISCRRHYPDKKVVLVRKEGTILIPCG
                     IPYIFGTVGGPENNVIPDGLLESNSIELIKGEAISVDRVKKIVTLKGQEQLQYDKLVF
                     AIGSLPIIPDISGINKKNVFPVRKEF"
BASE COUNT          392 a          186 c          265 g          417 t
ORIGIN      
        1 caaggcggcg agtaccgtga agatggcgtt gatatagtga caaatgataa tggctggata
       61 tagctccgga agagaaaaaa attggcttta tgctcggctc aatcttaccc tatttagtta
      121 tcatacttat ttttgtaggt gctatgcata ccgcagtgga tattactgcc ggagagaagg
      181 aaagaggtac tttggctact ttactggtca gccagctcag ccgtctggaa attgtatcag
      241 ggaaatattt ctcggtaatg gttatttcag ctataagtat gcttttaggg atggtagggc
      301 tcagcattgc ttttcttgtt cctgcttatt tattgggaga ggtatccata ataaatgtac
      361 atttttcttt taccctattc ctattatttc tgcttattct ggcacctctg gtaggatttg
      421 ccagtgccat acttatcctg ataggaattt ttgcccgcaa tagccgagag gcttcttctt
      481 ataccactcc tatttatatg gtagcaatat ttctgggtat gatttcttta agtcaaggaa
      541 tagaattagc ccaaccgctc ttttttatac caattttaaa taacagtttt gtctttaagg
      601 aattattaat gggaaccatt gattggaacc atatcacagc tactctgata agtaatatcg
      661 gaattgccct gctagcactt tttggaacag taaaattgtt caacagagag agtgtaattt
      721 ttcgaagtta gtttataata attgttaatt agtagtaatg gaaaagattt ataaaaatag
      781 agagagtttt tagcagataa agaaattaaa tggtaaaata ttaggaaaaa gaaaaaatta
      841 aatattattc ttatgtataa ggaggaaata gcgttatgaa aaaggcagat gtagttatac
      901 tgggaggttt atcaggtatt agcgcaggaa tcagctgccg ccgccattat cctgataaga
      961 aagttgtttt agtaagaaaa gagggaacaa ttcttattcc ctgcggaatt ccttatatct
     1021 ttggaacagt aggaggaccg gaaaataatg ttattccgga tggacttcta gaaagtaatt
     1081 ccattgagtt gattaaaggt gaggcaattt ctgtggacag ggtgaaaaaa attgttactt
     1141 taaaaggtca ggaacaactt caatacgata aattagtctt tgctatcggt tctttgccta
     1201 ttatacctga tatatctgga atcaataaga agaatgtttt tcctgtcaga aaggaatttg
//
LOCUS       DAOQWT010000511         1258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_142732_length_1258_cov_2.612635, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000511 DAOQWT010000000
VERSION     DAOQWT010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142732_length_1258_cov_2.612635"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKJ95_08305"
     CDS             complement(<1..560)
                     /locus_tag="PKJ95_08305"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006941308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNR66275.1"
                     /translation="MEKKSNMVTKGANRAPHRSLFYAMGYTKEDLEKPIIGVVNAFNE
                     IIPGHIQLNEIVQAVKIGVAAAGGIAMEFPAIGICDGIAMNHQGMKYPLASRELIADS
                     IEAVTMAHHFDGLVMVGNCDKIVPGMLMAAARLNIPAIYVSGGPMLTGYCQGHDSDLN
                     TVFEAVGKYNQEKISDKELLEIEYNA"
     gene            complement(674..1144)
                     /locus_tag="PKJ95_08310"
     CDS             complement(674..1144)
                     /locus_tag="PKJ95_08310"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNR66276.1"
                     /translation="MLILIGIILNILGIIGIVVPALPGITLNYLALILLYINKGEEVV
                     GLNTIIIFGLLTFLVALLDYILPLAGAKKSGASRTGIIFAALGMLIGLLFFPPFGIFF
                     GLLIGAFLGELIVGKTQSQALKAGMVTFLGILTSMVIKLMLAVVMTVYYIWHLF"
BASE COUNT          405 a          271 c          192 g          390 t
ORIGIN      
        1 caagcattat attcaatttc taacaactct ttatctgaaa tcttctcctg attatatttt
       61 cctacagctt caaaaacagt atttaaatcg gagtcatgcc cctggcaata accggtgagc
      121 attgggccac cactaacata gatggcagga atattgagac gtgctgcagc cataagcatt
      181 cccggaacga ttttatcgca atttcccacc ataaccaggc cgtcaaagtg atgagccata
      241 gtcacagctt caatagaatc tgctattaat tcacgacttg ccagagggta tttcattccc
      301 tgatgattca tagcaatccc atcacagata ccaatggctg ggaactccat tgctatccca
      361 ccggcagctg ctactcctat tttaactgcc tggacaattt catttaactg gatatgtcct
      421 ggaattatct cgttaaaagc atttacaaca ccaataatgg gcttctccag gtcttcttta
      481 gtatatccca tagcataaaa caaagaacga tggggagcac gatttgcccc ttttgttacc
      541 atattacttt tcttttccat atttttaccc ttcctagttt ttattttcaa ttctctttat
      601 tcttttataa tttttaaaat agagtatatt ttgactttac tattttttaa aatttttatc
      661 tttattgtca gatttaaaat aaatgccaga tataatagac ggtcatcacc actgccagca
      721 ttaatttaat gaccatagag gtaaggatcc ccaaaaaggt taccataccg gcctttaaag
      781 cctgtgactg ggttttccct actattaatt ctcccagaaa agcaccgatt aaaagaccga
      841 aaaaaatgcc aaatggcggg aagaacaata agcctatcag cattcccagg gctgcaaaaa
      901 taatgccagt acgagatgca ccagattttt tggcacctgc taagggtaga atataatcaa
      961 gcagggctac cagaaaagtt aacaggccaa atattataat ggtatttaaa ccgactactt
     1021 cctccccctt gttaatataa agcaaaatca gggcaagata gttcagtgtt atacctggca
     1081 aagccggaac tactatacca ataataccca gaatgtttag aattattcca attaaaataa
     1141 gcaagtttta ttccctcagc aaatgattga attacccgat acagtttcaa ttatcacaaa
     1201 cctgaataac ttatatttta catcttttat tttaactcgt tgaaaaaatt gatctgta
//
LOCUS       DAOQWT010000512         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_143078_length_1256_cov_2.770192, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000512 DAOQWT010000000
VERSION     DAOQWT010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143078_length_1256_cov_2.770192"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PKJ95_08315"
     CDS             <1..218
                     /locus_tag="PKJ95_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNR66277.1"
                     /translation="CQGTRWTDLFIYPGYHFKVINALITNFHLPRSTPLMLASAFAGK
                     ELLFQAYYEAIREGYRFYSFGDAMFIM"
     gene            338..>1256
                     /gene="tgt"
                     /locus_tag="PKJ95_08320"
     CDS             338..>1256
                     /gene="tgt"
                     /locus_tag="PKJ95_08320"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNR66278.1"
                     /translation="MPIEFKITKEINKINNRARLGLIKTAHGLVETPVFMPVGTQATI
                     KGILPKEIDALGFPIILSNAYHLFLRPGYQLISQCGGLHKFMGWNRAILTDSGGFQVF
                     SLGKINKINNDGVTFQSYIDGSSHFINPEKAMEIQMALGSDIAMVFDQCVPYPSNRYE
                     TLLAAERTYEWAKECLKYHNKKEQALFAIIQGGLFHDIRKENTLKLIELDFPGYALGG
                     LSVGEPKPLMNEMLDYIVPLLPADKPRYLMGVGAPESIIEGVMRGIDMFDCVLPTRNG
                     RNGCLFTHNGKISITNARYKEELRPIDQKC"
BASE COUNT          410 a          195 c          272 g          379 t
ORIGIN      
        1 tctgtcaggg cacacgctgg actgatttgt ttatttatcc gggctatcat tttaaagtaa
       61 taaatgcctt aattactaat tttcatttac cgcggtcaac tccccttatg ttagcttcgg
      121 catttgccgg gaaagaattg ctttttcaag catattatga ggctatcaga gagggctatc
      181 ggttttatag ttttggagat gctatgttta ttatgtgatt gtagtcagat agcagatatt
      241 taatatagtt ttctggatgc actgaattat ccttaatgtt taagaatcga aaataagata
      301 gtgaagtttg aagttatgct aataaaagaa agataaaatg ccaatcgaat ttaagattac
      361 caaagaaatc aataaaataa ataatagagc aaggctgggg ttgattaaaa cagctcatgg
      421 tttagtggaa accccggtat ttatgccggt aggaacccag gctactatca agggaattct
      481 gcccaaggaa atagatgcac tgggcttccc gataatactc agcaatgctt atcacctctt
      541 tttaagacca ggatatcagc ttataagtca atgtggtgga ttacacaagt ttatgggttg
      601 gaatagagcg attttgactg acagtggagg ttttcaggta tttagtttgg gaaaaataaa
      661 taagatcaat aatgatggag ttacctttca gtcctatatt gacggttcaa gtcattttat
      721 taatccagag aaggcaatgg agatacaaat ggctttaggt tcagatatcg ctatggtttt
      781 tgatcaatgt gtaccctatc cttctaacag gtatgaaaca ctattagctg cagagcgaac
      841 ctatgaatgg gcaaaagaat gcctgaaata ccacaacaaa aaagagcagg cgttatttgc
      901 catcattcag ggtggtcttt ttcatgatat ccgaaaagaa aacactttaa aactaattga
      961 actggatttt cctggttatg ccttaggcgg attgagtgta ggagaaccaa aacctttaat
     1021 gaatgaaatg ttagactata tagttccttt actgccagca gataagccaa gatacctgat
     1081 gggagtggga gctcctgaat caattattga aggggtaatg agagggatag atatgtttga
     1141 ttgtgtatta cccaccagaa atgggaggaa tggatgcctc tttactcata atgggaaaat
     1201 atcaattacc aatgccaggt ataaggaaga actaaggcca attgatcaaa aatgtc
//
LOCUS       DAOQWT010000513         1254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_143443_length_1254_cov_2.984153, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000513 DAOQWT010000000
VERSION     DAOQWT010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143443_length_1254_cov_2.984153"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKJ95_08325"
     CDS             complement(<1..326)
                     /locus_tag="PKJ95_08325"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNR66279.1"
                     /translation="MSKVFKVQATWLSGTQVESEAREFKMLLDKPKEENGTDLGPKPS
                     EVALQALGSCLIFAYLYAANRLEVAIDGLKVNIEGEMVPGGWRDEDNDKRTGFKNVRF
                     EVEVKT"
     gene            597..>1254
                     /gene="pgi"
                     /locus_tag="PKJ95_08330"
     CDS             597..>1254
                     /gene="pgi"
                     /locus_tag="PKJ95_08330"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379831.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNR66280.1"
                     /translation="MKEGEGYLREAYPVWKLLEEHYEKIKKVHLRQLFEDDPQRGEKM
                     VVEDEGIYFDYSKHRITSETLNLLLQLAEQSALQGHIEAMFSGEKINISEDRAVLHIA
                     LRSPRGDSIIVDGEDAVVKVHEVLHKMANFASQIRSGQWKGFTGKSIRNVISIGIGGS
                     NLGPEMAYQALRYYSIPEIKFAFLSNIDGTDFYEKVSGLNPEETLFIISSKSFTTPET
                     M"
BASE COUNT          401 a          234 c          246 g          373 t
ORIGIN      
        1 tcagttttga cctctacttc aaagcgtaca ttcttaaaac cggtgcgctt gtcattatct
       61 tcatccctcc agccaccagg aaccatctct ccctcaatat ttactttcaa accatcaata
      121 gctacctcta acctgttagc agcatagagg taggcaaaaa tgagacagct acccagagcc
      181 tgtagtgcga cctcagacgg tttaggtcct aaatcggtac cattttcttc ttttggttta
      241 tccaaaagca ttttaaattc ccttgcctca ctttcaacct gcgttccgga caaccaggtc
      301 gcctgtacct taaagacctt tgacataatc taaatccctc cactttcata atttacatac
      361 attatatatg aatctccatt tgtttaatag taaataagat tttgaaacca caggtagcat
      421 ttatattagg aaaagagctt tcccattctt taagcaaaaa gaaagggttc taaaaaagta
      481 tgttttaaaa aagggtataa tagtaaataa ttgttaaaac aaaatgagag gtattataat
      541 aatgataaac caatcatttt tatgaaaagt gagaattttg ttgttggagg tttgatatga
      601 aagagggaga aggatattta agggaagctt atcctgtctg gaaattactg gaagagcact
      661 atgaaaagat aaagaaagta catctgcggc agctttttga ggatgaccca caaaggggag
      721 aaaaaatggt tgtagaagat gaagggatct atttcgatta ttccaaacac agaattacct
      781 ctgaaacttt aaacctgttg ctacaactgg cagaacaatc tgctttgcag ggtcacattg
      841 aagctatgtt cagcggagaa aagattaata ttagcgaaga cagggctgta ttacatattg
      901 ccttgcgctc acccagagga gattccatta tagttgatgg cgaagatgct gtagtcaagg
      961 ttcacgaagt gcttcataag atggcaaatt ttgccagcca gatccgcagt ggtcaatgga
     1021 aggggttcac cggaaaatct atcaggaatg tcatcagtat tggtattggc ggttctaatc
     1081 tgggtccaga gatggcttat caggctttgc gttattatag tataccagaa atcaagtttg
     1141 cttttctttc caatatagat gggacagatt tttacgagaa agtatccggt cttaatccgg
     1201 aagaaacgct ttttatcatt tcttctaaaa gctttactac ccctgaaaca atgg
//
LOCUS       DAOQWT010000514         1254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_143478_length_1254_cov_2.432861, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000514 DAOQWT010000000
VERSION     DAOQWT010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143478_length_1254_cov_2.432861"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..>1254
                     /locus_tag="PKJ95_08335"
     CDS             68..>1254
                     /locus_tag="PKJ95_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNR66281.1"
                     /translation="MSKNLFRIFTFLSFFFLSAMFLLASAQQEDILGENIKPGIEGIW
                     EGRLQIPGIELSIVFHISATTEGKLIATMDSPDQGVTEIGVDRVTLKENDLNLQVQSI
                     GGTFQGKVNEDFTLIDGIWQQSGMSFKLLLQRAEKAIEIKRPQEPIKPYPYIEEEVKY
                     ENKTAGIVLAGTLTFPAEGGPFTAAILISGSGPQDRNETVFGHRPFLVLADYLTRRGV
                     AVLRFDDRGVGESGGNFTLATSEDFATDVLAAIAYLKTRKEINTRQMGLIGHSEGGLI
                     APMVAMQSSEVAFIILMAGPGLTGEEILHQQSALISRVRGSSEEEIIGNENYNKRVYS
                     LIKTEDNEKILAEKLHQLLLDHVNESSKEEKDRIGDIGVYLNAQQQSLLSPWFKYFLT
                     YDPG"
BASE COUNT          413 a          207 c          288 g          346 t
ORIGIN      
        1 aaaagatgtc taattaaatt aaataatagt caaaattaag ggaaatattt aaaaggatta
       61 attaaagatg agcaaaaatt tgttcaggat atttaccttc ctctctttct tttttttaag
      121 tgcaatgttt ctcttagctt cagcacagca agaggatatt ttaggagaga atattaaacc
      181 tggtattgaa ggcatttggg aaggaagact tcagattcct ggaattgagc tcagtattgt
      241 ttttcatatt tcagcaacta cagaggggaa attaattgcc actatggata gtccggatca
      301 gggggtaact gaaattggag ttgaccgggt tactttaaaa gaaaatgatt taaacctgca
      361 agttcaatca ataggtggta ctttccaggg taaggttaat gaagatttca ccctgattga
      421 tggaatctgg cagcaatcgg gaatgtcatt taaactttta ctacagcgtg cagaaaaagc
      481 aatagagata aaaaggcccc aggagccgat aaaaccctat ccttatattg aagaagaagt
      541 taaatatgaa aataaaactg ctggaattgt tctggcggga actctgacct ttcccgccga
      601 aggcggacca tttaccgcag ccatattgat tagcggttcc ggccctcagg acagaaatga
      661 aacagtattt ggtcatcgcc cctttctggt actggcagat tatttaaccc gtcggggggt
      721 tgcagtgcta aggtttgatg atagaggagt aggggaatct ggtggaaact tcactctggc
      781 taccagcgaa gattttgcta cagatgtcct ggctgccatt gcctatctta aaaccaggaa
      841 agagataaat acacggcaaa tggggcttat tggccacagc gaagggggat tgattgcacc
      901 gatggttgcc atgcagtcat cagaagtagc ttttattatt ttaatggccg gaccggggtt
      961 aacaggagag gaaattttac atcaacagag tgccttgata tccagggtaa gaggctcaag
     1021 tgaagaagaa attatcggaa atgagaacta caataaaaga gtatattctt taataaaaac
     1081 ggaagataat gaaaagatat tagctgaaaa actccatcag ctacttctgg accatgttaa
     1141 tgaatcaagt aaggaagaaa aggataggat tggtgacatt ggggtatatc tgaatgcaca
     1201 acagcaaagt ctactatctc catggtttaa atatttcctt acttatgatc ccgg
//
LOCUS       DAOQWT010000515         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_144415_length_1249_cov_3.051926, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000515 DAOQWT010000000
VERSION     DAOQWT010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144415_length_1249_cov_3.051926"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..>1010)
                     /gene="murA"
                     /locus_tag="PKJ95_08340"
     CDS             complement(29..>1010)
                     /gene="murA"
                     /locus_tag="PKJ95_08340"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNR66282.1"
                     /translation="ACLLTNEKSILKNVPQLKDVYSMIEVIKTLGVKVVWKDSNTLSI
                     DPDDFNNYEAPYELVKTMRASFLVMGPLLARLKKAKISLPGGCAIGARPVDFHLKGFR
                     ALGAEIITEKGYIQAEVKKLIGNDIYFDFPSLGATENIMMAAALAEGITMIENAAKDP
                     EVVELGTFLNKMGAKISGLGTDIITIKGVKELNGVNYLIIPDRIEAGTFMVAAAITGG
                     TVEIKHINPYYLKSCIVKLEEAGIKVDIEDDDSAIISNSGNIKSVDIKTMPFPGFPTD
                     LQAQFMALMTIANGTSIITENVFENRFAHAGDLRRMGADIKVEGRNCIIT"
     assembly_gap    1011..1020
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          381 a          253 c          174 g          431 t
ORIGIN      
        1 tcaaagaatt cttggcgctc ttgagaattt aagtaataat acagttcctg ccttcaactt
       61 taatatctgc tcccattctt ctcaagtcac cagcatgggc aaaacgattt tcaaagacat
      121 tttcagtaat aatactggtg ccattggcaa tagtcattaa agccataaat tgcgcttgta
      181 aatcagtcgg aaacccggga aaaggcattg tttttatgtc aactgatttt atatttccag
      241 aatttgaaat tattgcacta tcatcatctt caatatctac ttttataccg gcttcttcta
      301 attttacaat acaggatttt aaataatatg gattgatatg tttaatttcg acagttcctc
      361 cagtgattgc agctgcaacc ataaaagtcc ctgcttcaat cctatctggt ataatgaggt
      421 aattaactcc atttaattct tttactcctt taatagtaat aatatcggtt cccagaccac
      481 ttatttttgc tcccatttta ttcaaaaaag tacccagttc aactacttca gggtcttttg
      541 ccgcattttc aatcatagta attccttctg ctaaggcagc tgccatcata atgttctcgg
      601 ttgctcccag actaggaaaa tcaaagtaaa tatcattacc aattaatttt ttaacctctg
      661 cttgaatata tcccttttca gtaatgattt ctgcaccaag tgcccgaaat ccctttaaat
      721 gaaaatccac tggtctggca ccaatggcac atccgccggg aagagatatt ttagcttttt
      781 taagcctggc caacaaaggt cccattacca gaaaagaagc tctcatagtt ttaaccaact
      841 catatggtgc ctcataatta ttaaaatcat cagggtctat actaagagta ttactatctt
      901 tccacaccac ttttactcct aatgtcttaa taacttcaat catactgtag acatctttca
      961 actgtggtac attctttaaa atactttttt cgttagttag aagacatgca nnnnnnnnnn
     1021 gcagccataa tggaaagagc agaatttttt gcaccatcaa ttatcactgt accctgtaat
     1081 ggcacaccgc cggtaataaa aaacctatct ggattttgca atttttttca ccaatccttt
     1141 aattatatat tttagttaaa atagtagtta gtgttttatc ataaggaggt tccaatatac
     1201 catattcagt aattatagct gttatatttt cattgggagt tatatcaaa
//
LOCUS       DAOQWT010000516         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_144429_length_1249_cov_2.649916, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000516 DAOQWT010000000
VERSION     DAOQWT010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144429_length_1249_cov_2.649916"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PKJ95_08345"
     CDS             <1..560
                     /locus_tag="PKJ95_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66283.1"
                     /translation="MKREKERLKADSIILFSKQFVFPELIERASEEGIQLYELSILDY
                     IIKEHLKVPFSLLELRIAFNPLHHTRNNLFKLKRVRENQWKYWLLMKMVIVILGTART
                     QDAYMDINLLRKELEQIGNPFSNGNIDPVLVKRIINILSQEPFKLIELSPSGNIILAY
                     PDHLALQKIGNIWQYIMNKEEFNKT"
     gene            594..1229
                     /locus_tag="PKJ95_08350"
     CDS             594..1229
                     /locus_tag="PKJ95_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR66284.1"
                     /translation="MKIKWLGHSCFLITNNRGINILTDPYDETLGYRITKEKINIITI
                     SHEHYDHNNTMGIKGKPVVLKGAVNRDTHKIIFKGITSYHDSVFGKYRGNNTIFLIKS
                     DQLTLCHLGDLGHLLEVAQLEEINKVDILFVPVGGYYTLNHTQADQVIEQIKPSIVLP
                     MHYKTDAIKWSIDPLSFFLDKKQNIKIIGANSFDIEVESLPEQTTIYVLQY"
BASE COUNT          450 a          203 c          224 g          372 t
ORIGIN      
        1 ctatgaaaag ggaaaaagaa aggctaaagg ctgattccat aattctgttt tccaaacaat
       61 ttgtcttccc tgaactgatt gaaagagcca gcgaggaagg aattcagcta tatgaacttt
      121 ctatcttaga ttatattatt aaagaacacc tcaaggtccc cttttcctta cttgaactga
      181 gaattgcctt caatcctttg catcatacca ggaataatct gttcaaattg aaaagggtaa
      241 gagaaaacca gtggaaatac tggttgttaa tgaaaatggt aattgttatt ttgggaactg
      301 ccaggactca ggatgcctac atggatataa atctacttag aaaagaattg gaacagatcg
      361 gtaatccctt cagtaatggc aatattgacc cggttttagt aaaaagaata attaatatat
      421 tgagccagga accttttaaa ttaattgaat tatcaccatc aggaaatatt atactggctt
      481 atcctgacca tcttgccctg cagaagattg gtaatatctg gcagtatatt atgaataaag
      541 aggaatttaa caaaacttaa ccagaaaaat ttttcttaga ggtaaagaaa ttaatgaaaa
      601 taaaatggct gggacattcc tgttttctta ttaccaacaa tagaggtatc aatattctta
      661 ctgatcccta tgatgagaca ctggggtaca gaataaccaa ggaaaagatt aatattatta
      721 ccatcagcca tgagcattat gatcataata atactatggg aattaaaggg aagccggttg
      781 ttttaaaagg ggctgtcaac cgtgatacac ataaaataat ttttaagggc atcacttcct
      841 atcatgatag tgtctttgga aaataccggg gaaataatac catttttcta ataaaatccg
      901 accagttgac cttatgccac ctgggcgatt taggtcattt gctggaggtt gctcagctgg
      961 aagaaataaa taaggtagat atattatttg ttccggtagg tggctattat accctaaacc
     1021 atactcaggc agaccaggta atagagcaga taaagcccag tatagtactt ccaatgcatt
     1081 ataagacaga tgctatcaaa tggtctattg atccactctc atttttttta gataaaaagc
     1141 aaaacatcaa aataattgga gctaatagct ttgatataga agtagaatca cttccagagc
     1201 aaactaccat ttatgtatta caatattaaa ataatttaaa tcctgaata
//
LOCUS       DAOQWT010000517         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_144592_length_1248_cov_2.856664, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000517 DAOQWT010000000
VERSION     DAOQWT010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144592_length_1248_cov_2.856664"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKJ95_08355"
     CDS             <1..402
                     /locus_tag="PKJ95_08355"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264922.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNR66285.1"
                     /translation="IFIQPDQALREYSVKIKLSAIASIVTGKKIIMVDDSIVRGTTSR
                     KLVKLLKKSGAKEEHVRISSPPVDFPCKYGIDTPNKKELWTNHFSIEALRKLIEADSL
                     GYISLEGLTGVFQKNKPSDFCTACFSGEYPL"
     gene            441..>1248
                     /gene="purM"
                     /locus_tag="PKJ95_08360"
     CDS             441..>1248
                     /gene="purM"
                     /locus_tag="PKJ95_08360"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNR66286.1"
                     /translation="MSDTYKKAGVDIELANRIIGKIKPFIHTTHIPGVIGEIGHFAGL
                     FSLAQEKIVDPVLVSGTDGVGTKLKIANLMNKHDTIGIDLVAMCVNDIATCGAKPLFF
                     LDYLATGKLEEEVMVSIIKGIVQGCKIAGCALLGGETAEMPGFYNDGEYDLAGFAVGI
                     VDRKKLISGNNIVSGDTVIGLASSGLHSNGFSLVRKIFLAEGEYSLTKGVPTLNLSLG
                     EELLKPTRIYIPTILALLKEFQIKGIAHITGGGFLDNIPRILPENTAVSIN"
BASE COUNT          409 a          188 c          276 g          375 t
ORIGIN      
        1 atttttatcc agcctgatca ggcactcaga gaatactctg ttaagataaa actttcggca
       61 attgccagta ttgtaactgg gaaaaaaata attatggttg atgattcgat tgtgagaggc
      121 actaccagcc gtaaattagt aaaattatta aaaaagtcag gagcaaagga agaacatgtc
      181 agaattagct ctcccccggt agatttcccc tgtaaatatg gcatagatac accgaataaa
      241 aaagaattat ggactaacca tttttctata gaggcgctaa gaaagctgat agaagccgat
      301 tctctgggct atatcagttt ggaaggattg actggagttt ttcagaaaaa caaaccatct
      361 gatttttgca ctgcttgttt tagcggcgaa tatccattat aaaaaaagtt gtgaaaaaaa
      421 tgaaaggaag agtgtgtaat atgagtgata catataagaa agcaggagtc gatattgaat
      481 tagccaatag aatcattggt aaaattaaac ctttcattca caccactcat ataccaggtg
      541 ttataggtga gattggacat tttgcgggat tattttcttt agctcaggaa aagattgtgg
      601 acccggtcct tgtttctgga acagatggcg taggcactaa actgaagatt gctaatttaa
      661 tgaataaaca tgatactatc ggtatagatt tagtagcgat gtgtgttaac gatatagcaa
      721 cctgtggtgc caaaccgctc ttctttttag attatctggc cactggaaaa ttagaagaag
      781 aagtgatggt gagcatcatc aaagggatcg tacagggttg caaaattgct ggttgtgctt
      841 tattgggtgg agaaactgcg gagatgcccg gtttttataa tgatggagaa tatgatttag
      901 ctggttttgc agtaggaata gtagatagaa aaaaacttat ttcgggaaat aatattgttt
      961 caggcgatac ggtgatcgga ttggcttctt ctggtcttca tagtaatggt ttttcattgg
     1021 tgagaaaaat ctttctggca gaaggggaat atagtcttac aaaaggtgta ccaactttga
     1081 atctgagtct gggagaagag cttcttaaac ctaccagaat atatattccg acaattttag
     1141 ctttattaaa agaattccaa attaaaggta ttgctcacat taccgggggt ggttttctcg
     1201 acaatattcc tcgcatactg cctgaaaata cagccgttag tataaata
//
LOCUS       DAOQWT010000518         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_144772_length_1247_cov_3.163591, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000518 DAOQWT010000000
VERSION     DAOQWT010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144772_length_1247_cov_3.163591"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKJ95_08365"
     CDS             <1..402
                     /locus_tag="PKJ95_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66287.1"
                     /translation="SKSGVPMLKLKLKIVEGELKGRIIFDQLSFNCKRPKNFAVALMM
                     AKKTDKDFELDSGKVLGKLVYAKLEEENYEGNVRSKVAFDGYGKVPTETIIKTLEELK
                     LEKDEDDDEELTDNTDEEIEAVEDIYAKYNL"
     gene            457..>1247
                     /locus_tag="PKJ95_08370"
     CDS             457..>1247
                     /locus_tag="PKJ95_08370"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HNR66288.1"
                     /translation="MIKYSEDNIKSNNSINKLPEMTIGEQKSRKTHLQIYKKLNIPIL
                     TYPVGIKNPVVKGWNTADIKTLWKQQKEELKTSNLGMRLEDLLVIDIDDPVLAKEKLG
                     DLQLPVTSIVYRGEREPGKVLQWEERGHIYYKRPAWLKFKNSKKLPDLGIELRTGAGS
                     QNVIPDSIHPDGTRYKWHYSEFFFQNKLQEFPEDLFKEIINRANIFHEDETAEVKIIA
                     EEEATKEFYRQKKILDQLISKYGFTCEKGEFKHGGQALSQGINSP"
BASE COUNT          479 a          179 c          288 g          301 t
ORIGIN      
        1 tccaagtcag gagtacctat gttaaagctt aaattaaaga ttgtagaggg tgagctaaaa
       61 ggccgaatta tatttgacca gttatccttt aattgcaagc gaccaaaaaa ttttgcagtg
      121 gctttaatga tggcgaaaaa gacagataaa gactttgaac tggattccgg taaggttctg
      181 ggtaaactgg tatatgcgaa gctggaagaa gagaactatg agggtaatgt cagatccaag
      241 gttgcctttg atggctatgg taaagtaccg acagagacaa tcattaaaac actggaagaa
      301 ctgaaattgg aaaaagatga agatgatgac gaagaactga cagataatac tgatgaggaa
      361 atagaagctg tcgaggacat ttacgcaaaa tataatttat aatttcagat agagccctgg
      421 ttaatgccgg ggctcttgct acaggaggaa tgaaaattga taaagtattc tgaagataat
      481 ataaagagca ataacagtat aaataaactg cctgagatga ccatagggga gcagaaaagt
      541 agaaagaccc atttacagat atataaaaaa ctaaatattc caatactgac ttatccagtg
      601 ggaataaaaa acccggtagt aaaagggtgg aacactgcag atataaaaac tctgtggaaa
      661 cagcagaaag aagaactgaa aacatctaat ttaggcatga ggcttgaaga tttacttgta
      721 attgatatag atgatccggt cctagcaaaa gaaaaacttg gagacctgca attacctgtt
      781 acatctatag tttacagggg agagagggag ccggggaagg tacttcagtg ggaagaaaga
      841 gggcacatat attataaaag accggcatgg ttaaagttta agaattcaaa gaaactgcct
      901 gatttaggga tagagctaag aacaggggca ggaagtcaga acgtgattcc tgactccatt
      961 catccagacg gaacccgtta taagtggcat tactcggaat ttttctttca aaataagtta
     1021 caggaattcc cggaagattt attcaaagag ataataaata gggctaatat tttccatgaa
     1081 gatgaaacag cagaagtaaa gattatagca gaagaggaag caacaaaaga attttacaga
     1141 caaaagaaga tcttggatca gcttatttca aaatatggct ttacgtgcga aaaaggagaa
     1201 tttaaacatg gagggcaggc ccttagtcaa ggaataaatt ctccttt
//
LOCUS       DAOQWT010000519         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_145582_length_1243_cov_2.886364, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000519 DAOQWT010000000
VERSION     DAOQWT010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145582_length_1243_cov_2.886364"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PKJ95_08375"
     CDS             <1..804
                     /locus_tag="PKJ95_08375"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNR66289.1"
                     /translation="NGPQVGNLMVQQKVAQDIVPAHPMDVVGAMTQGQIGYMLQQTLM
                     NHLKEKKIDKPVCTLLNQVLVDRNDPEFEGNKASKPVGNFLTKGEAEELKKNNPDYIV
                     KKVKPNGERCWRRVVPSPDPIANLEGNVIRKLVEAGVIVIASGGGGIPVLEDEQGRLK
                     GIEAVIDKDLAGERLAEIVKANILFVLTDVEKVKLNFGKPDEEELGKLTYQEAKNYYD
                     EGHFLAGSMGPKVKACLRFLEYGGEKAIISSLDKVLDAYRGITGTIIEK"
     gene            973..>1243
                     /locus_tag="PKJ95_08380"
     CDS             973..>1243
                     /locus_tag="PKJ95_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66290.1"
                     /translation="MIQFQNRLLNSLVSIFFLSLSIFFRKKSLMIFTNRLNLILWFMI
                     IINIILFLNLVFFQKESFRKIRAISFPELRLIFLAGTLLLSFYTLA"
BASE COUNT          393 a          190 c          260 g          400 t
ORIGIN      
        1 aatggacccc aggtagggaa tcttatggta cagcagaagg ttgcccagga tattgtaccg
       61 gctcatccca tggatgtggt tggtgctatg actcagggtc aaataggcta tatgttgcag
      121 cagactctaa tgaatcactt aaaagagaag aaaattgata agccggtctg tactttattg
      181 aaccaagtac tggttgatcg caacgaccct gaatttgaag ggaataaagc ctcaaaacct
      241 gtcggtaatt tcctaactaa aggagaggcg gaagaactaa aaaagaataa ccccgattat
      301 attgtcaaaa aagtaaaacc caatggggaa cgttgctgga gaagagttgt cccttctccc
      361 gacccaatag ccaatctgga aggaaacgtt attcgcaaac tggttgaagc aggagttatt
      421 gtaattgctt caggtggtgg tggcataccg gtattggaag atgaacaggg cagactaaaa
      481 gggatcgagg cagtgataga taaagatctg gccggggaaa gactggcaga gattgtcaaa
      541 gcaaatatcc tctttgtact taccgatgta gaaaaggtta aactcaattt tggaaaaccg
      601 gatgaagagg aattggggaa gcttacctat caggaagcga aaaattatta tgatgaggga
      661 cattttctgg ccggtagcat gggtccaaaa gtaaaagcct gtcttcgttt tttagaatat
      721 gggggagaaa aggcgattat ctcttcctta gacaaagttc ttgatgctta tagaggcatt
      781 acggggacta ttattgaaaa ataaatctat agaatattgt caaaatgcca ttcattatca
      841 ttggatttaa aaatttcatt tggttaattt gttattcata atcttgaata tgtggatatg
      901 gtctattgat aattatgaat ttttaattta ctttttgttt tgaagtttag tttaaatttt
      961 attgaggcag atatgattca gtttcaaaat cgtttattaa acagtctggt atctatattt
     1021 tttctttcgc tttccatttt ctttcgaaaa aaatcattga tgatttttac aaaccgtctt
     1081 aatcttatct tatggtttat gattattata aatataatcc tttttttaaa tctggttttt
     1141 tttcaaaaag aatcttttag aaaaataagg gcaatttcct ttccagagtt acgattaatt
     1201 ttcctcgcgg gtactctttt actatcattt tacaccctgg cta
//
LOCUS       DAOQWT010000520         1237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_146746_length_1237_cov_3.134518, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000520 DAOQWT010000000
VERSION     DAOQWT010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146746_length_1237_cov_3.134518"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1077
                     /locus_tag="PKJ95_08385"
     CDS             172..1077
                     /locus_tag="PKJ95_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012571218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNR66291.1"
                     /translation="MSNHSHLHQQSNSNHSNKRIIIAIILNFLICLSEIIGGLLSGSL
                     SLLADAFHNFSDGMAILISYLARIIGMRSPDSRMTFGYKRAEILAALLNSSVIIVISF
                     YLFREAYLRFREPQKITVPLMLVVAVFGLIADLICVYLLHPETHRSLNMRSAYLHLLS
                     DTLSSVGVVLAGIIIYFYPVYFLDPLLTVVIAIFILVQGYQILKQSTLILMERVPAEV
                     DIEEIKKKIETIPGINSLHHIHIWQIDEQKYLFESHITLHQDMMISEADEIRKKVEKI
                     LADDYQIFHPHLQLEYSYCNNQECE"
BASE COUNT          417 a          192 c          217 g          411 t
ORIGIN      
        1 tataagatta attgatataa taatcagctg ctgaattaac aatagtgcat taatcaaaaa
       61 ttgtaaatat tgatataatg taaatattta aaaaatccga gcaaacaaaa actcactttc
      121 tatttgttat aatgaattca ggtttcttaa atattactaa aagagaaaaa tatgtctaat
      181 cattcacatc tacatcaaca aagtaatagt aaccatagta ataagagaat aattattgcc
      241 attatactta attttctgat ctgtctgtca gagataattg gtgggttatt atcgggtagc
      301 ctatccttac tggctgatgc ctttcacaat ttcagtgatg ggatggctat actgataagt
      361 tatctggctc gaataatagg catgcgttcg ccggattcca ggatgacctt cggctataaa
      421 agggcggaga tattagctgc attattaaat tcttcggtga tcattgtcat ttctttttat
      481 ctgtttcggg aggcttatct tcgatttaga gaaccccaga aaattactgt acccttaatg
      541 ctggtagttg cagtttttgg tttaattgct gatttaattt gtgtctattt gcttcatccg
      601 gaaacacatc gaagtctaaa tatgcgttca gcttatttac acctgttgag tgatacactc
      661 tcttcagttg gggtagtcct tgccggtatc attatttact tttacccggt ttattttctc
      721 gatccccttt taacagtagt tattgccata tttattcttg tccagggtta tcagatctta
      781 aaacagagca cacttatact gatggaacgt gttcccgcag aggtagatat agaagagata
      841 aaaaagaaga ttgaaaccat tccgggtatt aacagtcttc atcatattca tatctggcaa
      901 atagatgagc aaaaatacct ttttgaaagc cacattaccc ttcaccagga tatgatgatt
      961 agtgaggcag atgagatcag gaaaaaggtg gaaaagattc tggcagatga ttaccaaatt
     1021 tttcatcccc atcttcaatt agagtacagt tattgtaata accaggaatg tgaataatat
     1081 cactgaataa ctttaaaaga tttccattga caatttctaa aaaaaaggag ataaaataat
     1141 ataaggataa tacaaattat aaggaatagc ttataatgaa gtttttctat ctggagtatc
     1201 aggaaataaa aaaatttgtg gaggataact acaaagt
//
LOCUS       DAOQWT010000521         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_147893_length_1232_cov_1.959218, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000521 DAOQWT010000000
VERSION     DAOQWT010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147893_length_1232_cov_1.959218"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKJ95_08390"
     CDS             <1..348
                     /locus_tag="PKJ95_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66292.1"
                     /translation="GATVNDQKIKCSDCIFSTVLNSDKNLYRCDKGEMTDYNGLTERS
                     CYEFKHKTDPAEVLQIEDNKRRYNEALRDYASEVNREHSKSVMVETINKLNAMGKKAK
                     PEEIQRGFELCIT"
     gene            336..584
                     /locus_tag="PKJ95_08395"
     CDS             336..584
                     /locus_tag="PKJ95_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66293.1"
                     /translation="MHNIIEDPFLLELFKSQFPDLPVAVEQDKYNFLLSERDRLLRII
                     RSSPITNQENGACYVACLALDEVHKELEEMKMNGGCNL"
     gene            581..907
                     /locus_tag="PKJ95_08400"
     CDS             581..907
                     /locus_tag="PKJ95_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR66294.1"
                     /translation="MKEKEKPLLKQRGSINLNEVPEEEINRAMRSIDFVTQKMNMIFS
                     KPLTIGQENKEDEFLTTEEVMKLLKLSRATIYRRIKDGSIPYMKIGRRLRFHRGLIFR
                     KFYYGR"
BASE COUNT          445 a          182 c          250 g          355 t
ORIGIN      
        1 ggtgctacag taaatgatca aaaaataaaa tgttctgact gtatattttc cactgtctta
       61 aactcagata agaatttata caggtgtgat aagggggaaa tgactgatta taatggatta
      121 actgaacgtt cctgttatga gtttaaacac aagacagacc cggcagaagt gctacagata
      181 gaagataata aaagaagata caatgaagca ttaagggatt atgcatcaga ggttaataga
      241 gaacatagta aaagtgttat ggtagaaact ataaataaat taaatgctat gggtaaaaag
      301 gcaaaacctg aagagataca gagaggtttt gagttatgca taacataata gaagatcctt
      361 tcttattgga attatttaaa agtcaatttc cagacctgcc ggtagctgtg gagcaggata
      421 aatataactt ccttctgtca gagcgtgacc ggctgttacg gataataaga tcttctccca
      481 tcacgaacca ggagaacgga gcctgttatg tggcctgtct tgcactggat gaagttcaca
      541 aggagctgga ggaaatgaaa atgaatggag gctgtaactt atgaaagaaa aagaaaaacc
      601 tttattgaag caaaggggat cgataaattt aaatgaggta ccggaagaag agattaacag
      661 agctatgagg agcatagatt ttgtgactca aaaaatgaat atgatctttt caaaacctct
      721 gactattggc caggagaata aggaagatga atttctcacg actgaagaag taatgaaact
      781 attgaagctg agcagagcaa ctatttacag aagaattaaa gacggttcta ttccatatat
      841 gaagataggc aggagactcc gttttcacag aggtctgatt ttcaggaaat tttactatgg
      901 aaggtaatat ttacagataa ttaaagttat ttactgagag taaacttttt taatagaaaa
      961 agataaaaaa actcttactg gataaagttt tcctctgttg tggatttctt ttagattctc
     1021 ctcatggtgt gacgtgtgag gtctatgagt attcataata ttcatatcta tttatccatg
     1081 aggcccggca gaatttcttt ttaacttata ccactttaaa aacttcttat atattcccat
     1141 ctcttttata tagatctgtt acactgggca gatgaactgc aaaattaacg ggcttgttca
     1201 cacaaaaaat atacttccaa aacaccgcac tc
//
LOCUS       DAOQWT010000522         1228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_148667_length_1228_cov_2.143223, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000522 DAOQWT010000000
VERSION     DAOQWT010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148667_length_1228_cov_2.143223"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="PKJ95_08405"
     CDS             <1..905
                     /locus_tag="PKJ95_08405"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HNR66295.1"
                     /translation="LFLASFYIPLLGTLISFLCPLPIIILCLRHNVKVALLALFISFF
                     LVTLVAGPFQGLIAVLGFGILGITLGIVIKKKLSILEILLIGSFASFIAKILLMFIGL
                     WLMGVNPLLLGLEQIDQGINQSLNFYQNMGINPEQLETYKNSLLQSLELIKIAFPAML
                     ILSSVFDTIINYWVTERILKRLGYSMEPFTPFHQWRVGHSFFWSYLLGMFLLIINTKY
                     NLPVLQRIGVNIQLLFSIIFLITGLALIAHLFQYYKIKPFFFWVTCGIVFIQPLLSLL
                     VTWIGILDVWIDFRRLFFTEKRND"
     gene            977..>1228
                     /gene="rplI"
                     /locus_tag="PKJ95_08410"
     CDS             977..>1228
                     /gene="rplI"
                     /locus_tag="PKJ95_08410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNR66296.1"
                     /translation="MKVILKGNVDNLGYIGDVVEVNKGYARNYLFPRGLAIEHSSGNL
                     KSMEHLKKKEWEKRNRELLEAKELAAKLNQLNIVFKAKAG"
BASE COUNT          368 a          190 c          244 g          426 t
ORIGIN      
        1 tacttttttt agctagtttt tatattcctt tgctgggaac tttaattagc tttttatgcc
       61 ctttaccaat aattattttg tgtttgcgcc ataatgtgaa agtggcattg ctggcacttt
      121 ttatctcctt ttttttggtt acgctggtag ccggaccttt tcagggatta attgcagtac
      181 tgggttttgg gatattggga attaccttag gtatagttat aaagaaaaaa ctatccatcc
      241 tggagattct tttgataggc agctttgcct cttttattgc caaaatacta ttaatgttca
      301 taggactctg gctgatgggt gtcaaccctt tactgcttgg gttggaacag attgaccagg
      361 gtatcaatca aagcctgaat ttttatcaga atatgggtat taatcccgaa cagttggaaa
      421 cctataaaaa ttctctttta caatccttag aattaattaa aatagccttc ccggcaatgc
      481 taatacttag ttcggtcttt gatacgatta ttaattattg ggtaacagag aggatactga
      541 aaagattggg ttattcgatg gaaccattta ccccattcca tcaatggcgg gtaggacact
      601 ctttcttttg gagttatctg ctgggaatgt ttctgctaat tattaacaca aaatacaatt
      661 tgccagtatt gcaaaggata ggggtgaata ttcaattatt attctctata atatttttaa
      721 ttactggttt ggctttgata gcccatcttt ttcaatatta taagattaaa ccttttttct
      781 tctgggtaac ctgcggtatt gtctttattc agcccctgct ctcattattg gttacctgga
      841 ttggaatact cgatgtttgg attgattttc gcaggttatt ttttacagaa aagagaaatg
      901 attagaaaat gataaataca gaaatagaaa tattagggaa aaatgttttc tcacaaactt
      961 cagggaggta catccgatga aagttatctt aaaaggaaat gtagacaatt tgggctatat
     1021 tggcgacgta gttgaggtga acaagggata tgcccgaaat tatttatttc ccagaggcct
     1081 tgctattgaa cactccagtg gcaatttaaa atctatggaa catcttaaga aaaaagaatg
     1141 ggaaaaaaga aatcgggaat tattggaagc caaagagctg gcagctaagc ttaatcaact
     1201 gaatattgta tttaaagcaa aggcaggg
//
LOCUS       DAOQWT010000523         1225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_149305_length_1225_cov_2.252991, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000523 DAOQWT010000000
VERSION     DAOQWT010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149305_length_1225_cov_2.252991"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PKJ95_08415"
     CDS             <1..606
                     /locus_tag="PKJ95_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNR66297.1"
                     /translation="LMDSIEKRGYIPKSAVWVNAVTDNGTKQGNIIQYRLVAGRHRYE
                     ACLRIELKEIPCQLYYNLTDIEECEFDRIDNELDEHHKPFHFLDEAEHYKYLRDIKGW
                     SQRQIAEAKNISRAVVQYRLKISELSEDLKKIIRGGHHGIHLVERYMRELLKLSSIPH
                     QILVCKEIMEGQTDINVGNNQFQDQKNEVNLKCTPTKKLMT"
     gene            625..>1225
                     /locus_tag="PKJ95_08420"
     CDS             625..>1225
                     /locus_tag="PKJ95_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66298.1"
                     /translation="MNQLLALELQGKIAGEAIPFVTDKAELEDLLSTSSNEHGNNSNG
                     IGEPVTGQVFFENYYSVNDMSGINAKNEYSKQSQAKKISGNNKPAEENQEEPKAEQLI
                     FDIFATMSDEREEKGVRFGVKPLWIKHSGLVRGLSSGAYLLLIELIENDFRYKPKKDK
                     FFFIKIDEQYENCIDFLSYVTGVKPVTLVKKILPELEKFV"
BASE COUNT          448 a          177 c          255 g          345 t
ORIGIN      
        1 ttgatggatt ctatcgaaaa acggggatat attcctaaat ctgccgtctg ggttaatgct
       61 gttacagata atggaactaa gcaggggaat ataattcaat acaggcttgt agcaggtcgc
      121 cacaggtatg aggcatgcct gagaattgaa ttaaaggaaa ttccctgcca gttgtattat
      181 aacttaaccg atatcgagga gtgcgaattc gaccgaatcg ataatgaatt ggatgaacat
      241 cataaaccct ttcatttcct tgatgaagca gaacattata agtacttgag ggatataaaa
      301 ggctggtctc agagacagat agctgaagca aaaaatatat caagagcagt tgttcagtat
      361 agattgaaaa tttctgaact ttcagaggat ttaaagaaaa ttatcagggg tggacaccat
      421 ggtatccacc ttgtagaaag atacatgaga gagttactaa aactatcatc aatcccgcat
      481 caaatattag tttgtaagga gattatggaa ggtcagaccg acatcaatgt cggaaacaac
      541 cagttccaag accaaaaaaa tgaggttaat ttaaagtgta ctccaaccaa aaaacttatg
      601 acttaatcag atgtagaatt acgtgtgaat caacttttag ctctggaatt gcagggaaaa
      661 atagccggag aagctattcc ttttgttaca gataaagctg agttagaaga tctattatca
      721 acttcttcta atgaacatgg taataattca aatggcatag gagaaccagt tacaggtcag
      781 gtattttttg aaaattatta ttctgttaac gatatgtcag gaataaatgc taaaaatgag
      841 tattcaaagc aatctcaagc aaaaaaaata tcagggaaca ataaaccggc agaagaaaat
      901 caagaggagc caaaagcaga acaattgatt tttgatatat ttgctactat gtctgatgag
      961 agagaggaaa aaggtgtaag gtttggagtt aaacctttat ggataaagca tagcggtctg
     1021 gtacgtgggt tatccagtgg agcgtattta ctgttaatag aattaataga aaacgatttt
     1081 cggtataagc caaaaaaaga taaattcttt tttattaaga ttgacgagca atatgaaaat
     1141 tgcattgatt ttctttccta tgttaccggt gtaaagcctg ttacactggt aaaaaagata
     1201 ttaccggaac tggagaaatt tgttt
//
LOCUS       DAOQWT010000524         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_149871_length_1222_cov_2.976864, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000524 DAOQWT010000000
VERSION     DAOQWT010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149871_length_1222_cov_2.976864"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..>1222
                     /locus_tag="PKJ95_08425"
     CDS             207..>1222
                     /locus_tag="PKJ95_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNR66299.1"
                     /translation="MRRNLIGLRAVIIIAILMITVCLNAIAQVNVDDAKKTVIEWADV
                     NAEHISDVSHQIWEFAEIGLTEFKSSDLLVSELEKNGFTMDRGLAGMPTSFVGTYIHG
                     SGNPVIGILAEYDALPNGHSCGHNLFGTASVSSAIALKEAMEKHNIDGTIKLFGTPTE
                     DTHGGKVWMAREGVFDGCDVFLSWHPGTKNDCNYGSNLAVQILEIKYYGISSHAGAAP
                     EKGRSALDGLTISSIAMEFLREHMIEPMRIQYIITNGGQAPNSVPEFAQMRLVIRGPM
                     MKDIDELRSREGGIDDCLRAGALASGTDVTMQVVGAFYNKMPNRTGSDLIIENMNYVG
                     SPTFT"
BASE COUNT          387 a          178 c          296 g          361 t
ORIGIN      
        1 ccgaacggca cgtacggtgg tgtgagaggg ggaagagatt tccccctact cgattttaag
       61 aaacaattaa aaagaaagga ggcaaattta tgtaaatata caggaaataa ggcagtggta
      121 caattttatt aacggaaagg aatgaaagca atattatgaa aatgaaatct tatttaatga
      181 aaaaattaga aatgaaggag ataattatga gaagaaatct aattggattg agagcggtta
      241 ttatcatagc tattttgatg attactgttt gtttaaatgc tatagctcag gttaatgttg
      301 atgatgccaa aaaaacagta atagaatggg ccgatgttaa tgcagagcat atcagtgatg
      361 tttctcatca gatatgggag tttgccgaaa taggcttgac tgaatttaag tcatcagatt
      421 tattggtaag tgaattggaa aaaaatggtt ttacaatgga tagagggttg gccggaatgc
      481 caaccagttt tgtgggtacc tatatacatg gatcaggtaa tccggttatt ggtattttag
      541 ctgaatatga tgctttgccc aatggacata gctgtggaca taatctattt ggtactgcca
      601 gtgtttccag tgctatagcc ttaaaagagg caatggaaaa acacaatatt gatggcacta
      661 taaagctgtt tggtacccct accgaggata ctcatggtgg aaaagtctgg atggcacgag
      721 agggagtttt tgacggttgt gatgtctttc taagctggca tcccggtact aaaaatgatt
      781 gtaattacgg ttctaatctg gctgtacaga tattggaaat taaatactat ggaatatctt
      841 ctcatgcggg tgcggcacct gaaaaaggac gctcagctct agatgggtta acgattagtt
      901 ctattgccat ggaattctta agagaacata tgattgagcc tatgagaata cagtatatta
      961 ttactaatgg tggacaagcg ccaaattctg ttcccgaatt tgcccagatg agattagtaa
     1021 ttcgtggccc aatgatgaag gatattgatg aattgcgttc tagagaaggt ggaatagacg
     1081 attgcctgcg tgctggtgcc cttgccagtg gtacagatgt aactatgcag gttgttgggg
     1141 ctttttataa caagatgccc aatcgcacag gttcagactt gataattgag aatatgaatt
     1201 atgtagggtc tcctaccttc ac
//
LOCUS       DAOQWT010000525         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_150116_length_1221_cov_2.544597, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000525 DAOQWT010000000
VERSION     DAOQWT010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150116_length_1221_cov_2.544597"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /gene="dut"
                     /locus_tag="PKJ95_08430"
     CDS             complement(<1..434)
                     /gene="dut"
                     /locus_tag="PKJ95_08430"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNR66300.1"
                     /translation="MENNLNIDEVEVKIERTKGCEGLPLPKAASKHASGIDLHSAEGK
                     TIEIKPGERKLISTGIKLALPAGYEAQVRPRSGLANGYGITVLNSPGTIDADYRGIIK
                     VILINHGKESFYINQGDRIAQLVIQKIVRPVFIEIQELDSTK"
     gene            complement(467..>1221)
                     /locus_tag="PKJ95_08435"
     CDS             complement(467..>1221)
                     /locus_tag="PKJ95_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNR66301.1"
                     /translation="PVAGVAMGLVKENEKYEILTDILGIEDHYGDMDFKAAGSADGLT
                     AVQMDLKIDGVSVDILRDIFERSKKGRLYILEKMNATISKPKAELSSYVPKMLIIKVS
                     TDKIGNVIGPSGKNIKKIIEETGTCIDIKDNGEIFITADAMEKIEKAKYLIGGLVREV
                     KAGEIYNGKVKRLTKYGAFVEILPGVEGLLHISNLSYSHVGKVEDVLKADDEIQVKVI
                     GIDQQGKIDLSKKDLMPKPEKDNKNYKNRYHK"
BASE COUNT          333 a          280 c          168 g          440 t
ORIGIN      
        1 cttttagtac tatccaattc ctgaatttct ataaataccg gtctgacaat cttttgaatt
       61 actaattgag cgatccgatc tccctggtta atataaaagc tttccttgcc atggtttatt
      121 aaaatgactt ttattatacc gcgataatca gcatcgattg ttcccgggga attcagtact
      181 gtaataccat aaccattggc aagaccactt cgaggtctta cctgggcttc ataacccgcc
      241 ggtagagcga gttttatacc ggttgaaatc aactttcgct caccaggttt aatctcaata
      301 gttttgccct ccgctgagtg caaatcaatt cctgaagcat gcttgctggc tgcttttggt
      361 aaaggcaacc cctcgcatcc ttttgttctt tctattttta cttcaacttc atcaatattt
      421 aaattatttt ccataagagt cagttattta attagttaaa agtagattat ttatgatatc
      481 gatttttgta atttttatta tctttctcag gtttaggcat caaatctttc ttacttaaat
      541 caattttgcc ctgctggtct attccgataa cttttacctg aatctcatca tctgccttta
      601 aaacatcctc tacttttcca acatggctat aagacagatt tgaaatatgc aacagaccct
      661 ctacaccagg cagaatttcc acgaatgcgc catattttgt taatctcttt accttaccgt
      721 tatatatctc gccagccttt acctctcgga ccaatcctcc aataagatat ttcgcttttt
      781 caatcttttc catagcatca gcagtgataa aaatttcgcc attatcttta atatctatac
      841 aggtaccggt ctcttctatt atcttcttta tatttttacc actgggtcca ataacatttc
      901 caatcttatc agtgcttacc tttatgataa gcattttggg aacataactg gataattctg
      961 ctttgggctt agatatagtt gcattcattt tttccagtat atagaggcgt cccttctttg
     1021 acctttcaaa aatatctcgc aatatatcaa cagatactcc gtcaattttc aggtccatct
     1081 gaacagcagt taaaccatca gcagagcccg ctgctttgaa gtccatatca ccataatggt
     1141 cctcaatacc aagaatatca gttaatattt catatttttc attttcttta accaatccca
     1201 tggcaacacc agccaccggt t
//
LOCUS       DAOQWT010000526         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_150975_length_1217_cov_2.420826, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000526 DAOQWT010000000
VERSION     DAOQWT010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150975_length_1217_cov_2.420826"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="PKJ95_08440"
     CDS             <1..1196
                     /locus_tag="PKJ95_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNR66302.1"
                     /translation="LLIFVGYPVMNSELLLGRKTQLAAVGTFKKLAPGTPWVIVGFMG
                     VLAGFVILSYYSVVAGWALAYMFKSGVYMAVGADHGGIFVSSITSTWGGIFWHSVFML
                     LCIAVVAGGVEKGIEKWSLILMPVLVVLLIILIIRSVTLPGAGAGLSFYLTPNFSNFS
                     GAGFLAAVGQVFFTLSLGMGCMITYGSYLKKEEDIPLVARYVVISDTAIALLAGFVIF
                     PAVFAFGLEPGAGPGLTFITLPAVFAQMGAAGHIFGFIFFTLLMIAALTSAISLLEVV
                     TAYSIDEMKFDRKKATIVAGVLIWALGLFPTLGYSTLSHIKLIKGMDLLDSFDFFANN
                     MLLPLGGMLTAIFIGWYWGTDKALEEANISASRKLGSNYAFLIKYVVPIAIFVVFLNS
                     VGLLG"
BASE COUNT          276 a          251 c          275 g          415 t
ORIGIN      
        1 ttctattgat ctttgtaggc tatccagtaa tgaactcaga gctattgctg ggtcgtaaaa
       61 cacaattagc cgctgttggt accttcaaga aacttgctcc cggcactccc tgggttatag
      121 taggatttat gggtgtttta gctggatttg ttattctttc ctattattct gtcgttgctg
      181 gctgggcatt ggcctatatg ttcaaatcag gtgtctatat ggcagtaggg gcggaccatg
      241 gcggtatttt tgtcagctca atcacctcaa catggggtgg tattttctgg catagtgtat
      301 ttatgttact ttgtattgcc gttgttgccg gtggtgtgga aaaaggaatt gaaaaatggt
      361 ctttgatact aatgcctgtt ctggttgttt tattaataat tctaatcatc cgttctgtaa
      421 cactacccgg tgctggtgcc ggcctttcct tctatctaac accaaatttt tctaacttca
      481 gcggtgcagg attcttagct gccgtcgggc aggtcttctt tactttgagt ctgggcatgg
      541 gttgtatgat tacctatggc agttatctga aaaaagagga ggatattccc ctggttgcca
      601 gatatgtggt aatatcggat actgcaatag ctctattggc tggctttgtt atcttccctg
      661 cagtatttgc ctttggattg gaacctggtg caggacctgg ccttactttt atcacacttc
      721 ctgccgtctt tgcccaaatg ggtgctgccg gacacatctt tggtttcatt ttcttcaccc
      781 tcttaatgat agcagcctta acctctgcca tctcattact ggaagtggta accgcttact
      841 ccattgatga aatgaagttc gatagaaaga aggcaactat tgtagcaggt gtacttatct
      901 gggcgctcgg cctcttcccc accctgggat acagcactct ttcccatata aaattaataa
      961 aaggaatgga cttactggat tcctttgact tttttgccaa caatatgctg cttcctttag
     1021 gcggtatgtt aaccgccata tttatagggt ggtattgggg tactgataaa gctttagagg
     1081 aagcaaacat aagtgccagt aggaaattgg gctccaacta tgccttcctg ataaagtatg
     1141 tggttcccat cgccatattt gtagtctttt taaacagtgt aggtttactc ggatagaaac
     1201 gacattctcc tcgtttt
//
LOCUS       DAOQWT010000527         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_151089_length_1216_cov_6.157623, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000527 DAOQWT010000000
VERSION     DAOQWT010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151089_length_1216_cov_6.157623"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1216
                     /locus_tag="PKJ95_08445"
     CDS             <1..>1216
                     /locus_tag="PKJ95_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006105342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR66303.1"
                     /translation="SNVIYKVVNLIPDDNFRIAINEALGQPADYIPTIADLNGLTGAL
                     YAHWRNISSIEGAQYLTNLQELYLDHNQISDLNPLENLTYLQYMSLGVNQISDLSPLE
                     GLTNLYYLYLGRNQISDLRPLEDLIYLQELDLYGNQISDLSPLAGLTNLQKLYLWDNQ
                     ISDLSPLAGLTNLQWIYLEYNQISDLSPFEELNNLQILHLEYNQISDLSPLAGLTDLY
                     SLYLEHNQISDLSPLAGLTNLFYLYLDNNQISDLSPLVGLTNLQGLDLSSNQISDLSP
                     LGGLTNLGILDLDNNQISDLSPLAGLTNLQQLYLIGNQISDLSPLAGLTNLRGLVLSD
                     NPISYESMLLSYSWGLPWSTDEYNPLSPCYPDPSRNATNVLVNYPLSWQGNYNNSQDV
                     FYEVWLGVSPDSL"
BASE COUNT          338 a          228 c          238 g          412 t
ORIGIN      
        1 tctccaatgt tatctataag gttgtaaatc tgataccgga tgataatttt agaatagcaa
       61 taaatgaggc attaggtcaa cctgctgatt atatacctac tattgctgat ttgaatggat
      121 taaccggagc tttatatgca cattggcgga atatttccag tatagaaggt gctcaatatc
      181 tgactaatct acaagagctt tatcttgatc ataaccagat aagtgacctc aatcctttag
      241 agaatctgac ttatctgcaa tatatgtctc ttggtgttaa ccaaataagt gacctcagtc
      301 ctttagaggg gctgactaat ctatattatc tatatcttgg tcgtaaccag ataagtgatc
      361 tcagaccttt agaggatctg atttatctgc aagagctgga tctttatggt aatcaaataa
      421 gtgaccttag tcctttagct ggtctgacta atctgcaaaa gttgtatctt tgggataacc
      481 agataagtga ccttagtcct ttagctggtc tgactaatct gcaatggata tatcttgaat
      541 ataaccagat aagtgacctc agtcctttcg aggagctaaa taatctgcaa atactacatc
      601 ttgaatataa ccagataagt gaccttagtc ctttagcggg tctgactgat ctgtattctc
      661 tatatcttga acataaccag ataagtgacc ttagtccttt agcgggtctg actaatcttt
      721 tttatctata tcttgataat aaccagataa gtgacctcag tcctttagtg gggctaacta
      781 atctgcaagg gctggatctt agttctaacc agataagtga cctcagtcct ttaggggggc
      841 tgactaatct ggggattctt gatcttgata ataaccagat cagtgacctc agtcctttag
      901 ctgggctaac taatcttcaa cagttgtatc ttattggtaa ccagataagt gaccttagtc
      961 ctttagctgg tctgactaat ctgaggggac ttgttcttag tgataatcct atttcttatg
     1021 agtcaatgct gctttcttat agttggggtt taccttggtc aaccgatgaa tataatcccc
     1081 tttcgccttg ttatcctgat cccagtagaa atgctaccaa tgttttggta aattatcctt
     1141 tatcctggca gggaaattat aataatagcc aggatgtttt ctatgaggtc tggttggggg
     1201 ttagtcctga ttccct
//
LOCUS       DAOQWT010000528         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_151762_length_1213_cov_4.996546, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000528 DAOQWT010000000
VERSION     DAOQWT010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151762_length_1213_cov_4.996546"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKJ95_08450"
     CDS             complement(<1..240)
                     /locus_tag="PKJ95_08450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR66304.1"
                     /translation="MEQLSAVLIVKNEEKNIARCLKSISWVDEIVILDTGSSDATVNI
                     CKQFGAKIYYLDKWEGFGKAKQQAVDLATFDWILSI"
     gene            complement(241..1167)
                     /locus_tag="PKJ95_08455"
     CDS             complement(241..1167)
                     /locus_tag="PKJ95_08455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR66305.1"
                     /translation="MQKSGRLIEIYKYLNNTNRFIVKNFFYGFLASMINRHPKSVVIS
                     ANSTIFYELSHYLTKSYLINILHGFYGINPKIWLLATPKINKRIVVSQAVYDEVQQYY
                     KANGWEEKYNSRLKLIYNSVPLCDDISPKDYQAPLKIVFIARQAKEKRFYLYQQVARK
                     IIREFPKIEFYCIGDHINTEPVINLGEICDRQKLYETIKDFHILILCSISEGFGLVIA
                     EAMTCGLVPLATDVGGVRESFQDGINGFLIKSKEEQEIIDEFISDIKVLNSSKKLLQQ
                     MSNSAREYVQEHFNYDRFKVEYTKVVEEGRNR"
BASE COUNT          385 a          230 c          177 g          421 t
ORIGIN      
        1 aatagacaaa atccagtcaa aagtagcaag gtctactgct tgctgtttgg ctttgccaaa
       61 accttcccat ttatccaaat aatatatttt agctccaaat tgtttacaga tattcactgt
      121 tgcatcgctg gaaccggtat ccaaaatcac tatttcatcc acccaagaaa tagattttaa
      181 acagcgggca atattttttt cctcgttttt tacaatcaaa actgcagaaa gttgctccat
      241 ttaccgattt ctgccttctt caactacttt ggtgtattca actttaaaac ggtcataatt
      301 aaaatgctct tgcacatatt cccgagctga attactcatc tgctgtaata atttcttgct
      361 tgaattcaat accttaatat cagagataaa ttcatctatt atttcctgtt cttctttgga
      421 tttaattagg aaaccattta ttccatcttg aaaggattct ctcactcctc ctacatcagt
      481 agccaaagga accagaccac atgtcatcgc ttcagctatt actaaaccaa aaccttcgga
      541 aatagaacat agaattaaaa tatgaaaatc ctttattgtc tcatacaact tctgacggtc
      601 acaaatttcc cctaaattta ttacgggctc ggtattaata tggtcgccaa tgcagtaaaa
      661 ttctattttc ggaaattccc gtataatttt acgagctacc tgctgatata agtaaaatct
      721 tttctctttt gcctggcgcg caataaaaac aatttttagg ggtgcttgat aatctttagg
      781 ggaaatatca tcgcagagag gaacagaatt gtagattaat tttaagcgac tattatattt
      841 ttcctcccat ccattagctt tataatactg ttgcacttca tcatatactg cttgagagac
      901 aacaatacgt ttgttaattt tgggagtagc caaaagccag attttaggat ttatgccgta
      961 gaaaccatgc aaaatattaa ttaaataact cttagtaaga tagtgagata attcgtaaaa
     1021 tatagtgctg ttggcagaaa taactactga tttaggatgt ctgtttatca ttgaagccag
     1081 aaatccatag aagaaattct taactataaa acgatttgtg ttattaaggt atttgtatat
     1141 ttcaatcaat cttccgcttt tttgcattgc ctgacatttt gcgtattttt gtagaccaaa
     1201 aaccttttat tat
//
LOCUS       DAOQWT010000529         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_152836_length_1208_cov_3.977450, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000529 DAOQWT010000000
VERSION     DAOQWT010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152836_length_1208_cov_3.977450"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PKJ95_08460"
     CDS             <1..816
                     /locus_tag="PKJ95_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNR66306.1"
                     /translation="AEESTAWPMVSRPTYSGGLGFGMKWNMGWMHDTLSYFSRDPIYR
                     KYHHQELTFSLLYTFNENFILSLSHDEVTHGKKSLLEKMPGDDWQKFANLRLLFGYMY
                     AHPGKKLFFMGSEFGQRQEWQHDFPLDWNVLKLPSHQGMQRWIKDLNGFYRQEKALYE
                     VDFEYSGFQWIENNDREHSVLIFLRKNGNGNEKILIVCNFVPTPWYNYRIGIPERGIW
                     LECLNSDSSLYGGSNHGNMGRVSSRPIPMHGYDYSLNITIPPLGILFFKKQKK"
     gene            852..>1208
                     /locus_tag="PKJ95_08465"
     CDS             852..>1208
                     /locus_tag="PKJ95_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNR66307.1"
                     /translation="MNKNKYICIHGHFYQPPRENPWLEEIELQDSAYPYHDWNEKISE
                     ECYKVNAASRILDKDENIVKIVNNYNQISFNFGPTLLSWIKRHQPEVYRSIQSADKLS
                     QIKFNGHGSAIAQAYNH"
BASE COUNT          383 a          238 c          242 g          345 t
ORIGIN      
        1 gccgaagaat ccaccgcctg gccaatggtc tcccggccaa cttattccgg agggctcggc
       61 tttggcatga aatggaatat gggctggatg catgacaccc tgtcctattt cagtagagac
      121 cccatctatc gtaaatatca ccatcaggaa cttaccttca gtcttttata tacctttaat
      181 gaaaatttca tactatcact ctcccatgat gaggtaacac atggtaaaaa gtctttgctg
      241 gaaaaaatgc ccggggatga ctggcagaaa tttgccaatt tgcgccttct ctttggttat
      301 atgtatgccc atcccggcaa aaaactcttc tttatgggca gtgagttcgg tcagagacag
      361 gaatggcagc atgattttcc tttagactgg aatgtactta aattgccttc ccatcaggga
      421 atgcagagat ggattaaaga cctgaacgga ttctatcggc aggaaaaagc tctctatgaa
      481 gttgattttg aatatagtgg atttcaatgg attgagaaca atgaccggga acacagtgta
      541 cttatttttc taagaaaaaa tgggaatggg aatgaaaaaa tcttaattgt ctgcaatttt
      601 gttcccacac cctggtataa ttatcgtatc ggcataccgg aaagaggaat ttggttagaa
      661 tgtttgaaca gtgattcctc gttatatggt ggcagcaatc atggcaatat gggtagagta
      721 agcagcagac ctatccccat gcacggatat gattattccc taaatattac cattccacca
      781 ctgggtattc ttttctttaa aaaacagaaa aaataatctt attaactaac ttatcttgaa
      841 ggagtttaaa gatgaataaa aataagtata tctgtattca cggtcatttc taccaacctc
      901 ctcgtgaaaa cccctggttg gaagagattg agttacagga ttcagcctat ccttaccatg
      961 attggaatga aaagatatca gaagaatgtt acaaagttaa tgccgcctct cgtatcctgg
     1021 ataaagatga aaatattgtt aaaattgtaa acaattataa tcagattagc ttcaattttg
     1081 gaccaactct cctgtcctgg ataaaaaggc accagccgga agtttaccga tcaatacagt
     1141 cagcagataa actcagccag ataaaattca acggacatgg ttcggctata gctcaggcct
     1201 ataatcat
//
LOCUS       DAOQWT010000530         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_153314_length_1206_cov_2.399652, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000530 DAOQWT010000000
VERSION     DAOQWT010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153314_length_1206_cov_2.399652"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..>1206
                     /gene="kynU"
                     /locus_tag="PKJ95_08470"
     CDS             88..>1206
                     /gene="kynU"
                     /locus_tag="PKJ95_08470"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNR66308.1"
                     /translation="MENNFQLGIEFAKELDTKDELSSYKNRFYLKEDKIYMDGNSLGA
                     CSKDAEESLLNMLEVWKREKILMWNIEDGKYFKYPQFIGGLFSEMVNADKNELTCTNS
                     VTINIHQALSTFFKPTKERYKILVDDLNFPTDIHAVKSQLRLKGIGIEDGLKVVKSRD
                     GRIIEEDDIIEALTDEVAIALFSSVLYRSAQLLDMERITKAAHKKGILMGWDLSHSIG
                     SVPHDFKKIDPDFAIWCTYKHISGGPGSNAILYINKKHFGTEPGMAGWHGNKKETQFL
                     LSHIHTPETDVDAWLTGTPNILSMAPLEGIMRLFNEAGIENIRKKSLLLTDYLIFLIE
                     KRLEKYGFRNGTPVEHSRRGGHIALEHEEAYRICLSLKE"
BASE COUNT          422 a          209 c          249 g          326 t
ORIGIN      
        1 aactaataac tttaatagct ttctactttt taaaaaattc tactttggta taatagaaaa
       61 taactaatat tggaaggagc agtaaaaatg gaaaataact ttcaattggg tattgaattt
      121 gcaaaagaat tggatactaa agatgagcta agcagttata aaaaccgctt ttatttaaaa
      181 gaagataaaa tatatatgga tggtaattcc ctcggggcat gttctaagga tgccgaagaa
      241 tcccttctca atatgctgga agtttggaaa agagagaaga tactgatgtg gaatattgaa
      301 gatggaaaat actttaaata ccctcaattt ataggcggat tattttccga aatggtaaat
      361 gctgacaaga atgaactcac ctgcaccaat agtgtcacca tcaatataca tcaagcactt
      421 tcaacatttt tcaaaccaac taaagaaagg tacaaaatac tcgttgatga tttaaacttt
      481 cccaccgata ttcatgccgt aaaaagccag ctcagattaa agggaattgg gattgaagat
      541 gggctaaaag tagtaaaaag cagggatggc aggattatcg aagaagatga tattattgaa
      601 gcacttaccg atgaggtagc catagccctc ttctccagtg tattgtacag gtctgcccag
      661 ttgctcgata tggaacggat aaccaaagca gcacataaaa aaggtatctt aatgggctgg
      721 gatttgagtc attctatagg ttcggttccc catgacttta aaaagattga cccggatttt
      781 gcaatatggt gtacttataa gcacatttca ggtggaccgg gctccaatgc catcctctat
      841 atcaacaaaa agcattttgg taccgaaccg ggtatggctg gctggcatgg taacaaaaaa
      901 gagacacaat tcctactctc ccatatacac acacctgaga cagatgtgga tgcctggctg
      961 acaggtactc ccaacattct ctctatggca ccattggaag gaattatgag actatttaat
     1021 gaagcaggta tagagaacat tagaaagaaa tctttactgc ttacggacta tctcatattc
     1081 ttaattgaaa agaggctgga aaaatatgga tttagaaatg gaacaccggt agaacactcc
     1141 aggcgtggcg ggcatattgc cctggagcat gaagaggcat atagaatatg tctttcctta
     1201 aaagag
//
LOCUS       DAOQWT010000531         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_153656_length_1204_cov_5.167972, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000531 DAOQWT010000000
VERSION     DAOQWT010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153656_length_1204_cov_5.167972"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PKJ95_08475"
     CDS             complement(<1..232)
                     /locus_tag="PKJ95_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66309.1"
                     /translation="MNLLENLYKSLSHEKIWGPDGLTPLNNFKANSGGYTACCPNPAH
                     PEKKPSFLMKEGIPAGKCQSCGYHLTWFDAVAI"
     gene            complement(491..>1204)
                     /locus_tag="PKJ95_08480"
     CDS             complement(491..>1204)
                     /locus_tag="PKJ95_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66310.1"
                     /translation="KTTVYRYIKQGKLKGEIVKQHGKELIYVANKDLEEFLGETVKQH
                     ETTWNPTYGKTWENMEQHGVTIEEIVKKAINEAVGQRETQLMKRIEDQALYKLGAVEK
                     ENLFLRERLETIRQENELLREQIKALPGPVELENKENIILLLQKEKEEQTRKTEGLNQ
                     VLLDNANNIKELTKEKDNFQSALKEQEATIREKERALKDLEEIHRAELELAEKKQMEI
                     SEAWKKELELAKKPWWKFW"
BASE COUNT          270 a          240 c          194 g          500 t
ORIGIN      
        1 ggatggctac tgcatcaaac catgtgagat gatatccgca ggactggcat ttgccggcag
       61 gtatgccttc tttcatgagg aaggatggct ttttttctgg atgtgccggg ttagggcaac
      121 aggcagtata accgcctgag ttggctttga aattgtttaa aggggtgagt ccgtccgggc
      181 cccatatttt ctcatgggag agagatttat ataaattttc taaaaggttc atgattgaaa
      241 ttctcctctt ttacaaagtt ttttatctct tagaaggtga tttcaatttt ccctagaaaa
      301 ttatatctgt cataatatat ttcactaggg tcaattgaat agatctaaaa aagtaggtac
      361 cgaggttcgc agcctcggtt ctgcaatttt agactatttt ctccttcatt cttttttcaa
      421 aagttcttcc tgagtattct attttgttat tgtttatcct ttactgaaaa aagattttta
      481 aatcccataa ttaccagaat ttccaccagg gcttttttgc cagttccaat tcttttttcc
      541 aggcttccga tatctccatc tgtttttttt ctgccagttc caattctgcc cggtgaattt
      601 cttccaggtc tttcagtgct ctttcctttt ctcttatagt tgcttcctgt tcttttaatg
      661 ctgattggaa attatctttt tctttcgtta gttcttttat gttattggca ttatcaagca
      721 agacctgatt taaaccttct gtctttcggg tttgctcctc tttttctttt tgtagcagta
      781 agataatatt ttctttattt tctaattcta ccggaccagg tagagcttta atctgttccc
      841 ttagaagttc attttcctgt ctaatagttt ccaatctttc tcgtaaaaat aaattttctt
      901 tttctacagc tccaagctta taaagtgcct ggtcttcaat tcttttcatt agctgtgttt
      961 ctctctgacc tacggcttca ttaatagctt tttttactat ttcttctatt gtaacaccat
     1021 gctgttccat gttttcccat gttttcccat aggtgggatt ccatgttgtt tcatgttgtt
     1081 tcacagtctc tcctaaaaac tcttcaaggt ctttattagc aacgtaaatt aactctttcc
     1141 catgttgttt cactatttcc ccttttaatt ttccttgttt tatataacgg taaacagtgg
     1201 tttt
//
LOCUS       DAOQWT010000532         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_153678_length_1204_cov_3.434291, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000532 DAOQWT010000000
VERSION     DAOQWT010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153678_length_1204_cov_3.434291"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            446..601
                     /locus_tag="PKJ95_08485"
     CDS             446..601
                     /locus_tag="PKJ95_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66311.1"
                     /translation="MNNAGVKILEESLMVSVIVKVMAIILPAALVVPAVGVEIVLPVA
                     IDISTFF"
     gene            707..>1204
                     /locus_tag="PKJ95_08490"
     CDS             707..>1204
                     /locus_tag="PKJ95_08490"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNR66312.1"
                     /translation="MKIARVAEMQNMDREAIQKYGISDELLMENAGLAVYYTLAKEFN
                     IKDSSFLIFCGSGNNGGDGLVIARKLYSAGAQVRICLLDNPEKLKGATQQNWQIIKEL
                     KLPTFFTPSVIELDSLIRPGDLIIDAIFGTGLTREVSGYYYQIIQTINKKNNPVISID
                     IPSGIN"
BASE COUNT          400 a          187 c          217 g          400 t
ORIGIN      
        1 tggttcaatt tttgataagt tacttatttc tgatttagaa aaaagtattt tattgcgaaa
       61 acggtaataa tagaccatag tttttccctt cttatcctta attcttataa attgagcaat
      121 aagaatctat caaccagctt acttcaatca ttttccttta atatgaataa cagttaggaa
      181 atatcttatt gtataaacat attttactat ctttatagcg aaaatccaac cactgtttca
      241 aacagcatta ttttgtttaa tttttatcct aaaatgtgat aaaattattc agggtgcttt
      301 aaatgtatta tagttatttt tcaaattatt tgtctttcag gaaggaattt tcttatgccc
      361 aattatcaat atgaatgcct ggactgctcc tcgctctttg tgataagggc ttcaattcag
      421 gatatggaaa agggtaactt ttcctgtgaa caatgcggga gtaaaaatat tagaagaatc
      481 tttgatggtt tcggttattg taaaggtgat ggcaattatt cttccagcag ctctggttgt
      541 gccagctgta ggggtggaaa ttgttctacc tgtagccatt gatatttcaa cattttttta
      601 gctaattttc agaaaaatat ttacttttgt atgaataata gaatttatta aagcacctat
      661 aaatgtattt atgtttaaat tcaaagctgt ccaggagaaa aaaagaatga aaatagcacg
      721 agtggcagaa atgcagaata tggaccggga agcaattcaa aaatatggga taagtgatga
      781 gcttttaatg gaaaatgccg gcctggctgt ttactatacc ctggctaaag agttcaatat
      841 taaagatagc agttttctaa ttttctgtgg aagcggcaat aatggaggag atgggttggt
      901 aatagccaga aaactatatt cagccggggc acaggtcagg atatgtctcc tggataaccc
      961 ggaaaaactg aaaggggcta ctcagcaaaa ctggcagatt attaaagaat taaaactacc
     1021 cacattcttt actccttctg tcatcgaact tgattcctta ataagacccg gcgaccttat
     1081 cattgacgcc atatttggta ccggtttaac cagggaagtt agcggatatt actaccaaat
     1141 aatccaaaca attaataaaa agaataatcc ggttatcagt atagatattc cttccggaat
     1201 aaat
//
LOCUS       DAOQWT010000533         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_155240_length_1197_cov_3.553415, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000533 DAOQWT010000000
VERSION     DAOQWT010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155240_length_1197_cov_3.553415"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..>1197)
                     /locus_tag="PKJ95_08495"
     CDS             complement(85..>1197)
                     /locus_tag="PKJ95_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66313.1"
                     /translation="VGETGLSRQIPVYIADFFEPGCLECEKAARLLEQIAFQYPQVEI
                     QKFDISTPEGVTIAEQLGEIYGVSEYDRLLVPIIYIGDSYFLREHINYDNLSNLIEEI
                     KDKEFPPPWEKIEEDISSVQQRLVERFQSFGVGAIAISGLIDGINPCAFATIIFFISY
                     LALIKRTGRDLLLVGSMFTLAIFLTYFLIGAGALKAVTTLSFLPLARQIFIFVTAGIG
                     IILGTLSLLDYLKFKKTGQASDATLQLPPRIKQMIHSTIRKNVKTRNFVIMAAVTGFM
                     VSILELACTGQIYLPTLIFISNVPEFRANALIYLLLYNLMFVVPLILVFLFTYWGTSS
                     QRWAELNRQHFGAIKLIMTFLFFGLAILLITTGLLF"
BASE COUNT          409 a          232 c          220 g          336 t
ORIGIN      
        1 gtatagattt aggcagaaag cagcataata attaagtatt cccctgtttt tttaatatat
       61 taagtaaact tcacttttcc tttattagaa gagcaatccg gtggtaatta agagaatagc
      121 caacccgaag aataaaaaag tcataattaa ttttatggca ccaaaatgct gcctgtttaa
      181 ttcagcccat ctttgagagc tggtacccca gtaggtaaac aaaaagacca gtattaaggg
      241 taccacaaac atcagattgt ataataaaag ataaataagg gcattggctc tgaattcagg
      301 aacattgctg ataaagataa gggtaggcaa atagatctgt ccggtacagg ctaattctaa
      361 tatagaaacc ataaagccgg ttactgcggc cattataaca aaattacgcg tttttacatt
      421 ttttcgtata gtagaatgaa tcatctgttt tatacgagga ggtagctgta gagtagcatc
      481 actagcctgt ccggtcttct taaattttaa ataatcgagc agactgagtg tccccagaat
      541 aataccgata ccggcagtga caaagataaa aatctgtctg gccagcggca aaaaagaaag
      601 agtagtcact gctttaagag cccccgcgcc aattagaaaa taagttaaga aaatagccag
      661 ggtaaacata ctacccacca aaagcaaatc tctgcccgtt cttttaatta aagccaggta
      721 ggaaataaaa aagataatcg tggcaaaggc acagggattt atcccatcga tgagaccgct
      781 tatggcaata gcccccactc caaaagattg aaatctttcc acaagacgct gttgaactga
      841 agaaatatct tcttctattt tttcccaggg tggagggaat tctttgtcct ttatctcttc
      901 aatcagattt gagaggttat cgtaattaat atgttctcgt aaaaaataac tatcaccgat
      961 ataaattatg ggcaccaaca accggtcata ttctgatact ccatagattt ctcctagttg
     1021 ttcagctata gtaacacctt ctggagtaga gatatcaaat ttttgaattt ccacctgggg
     1081 atattgaaaa gcaatttgct ctaaaagcct tgctgctttt tcacattcaa gacagcccgg
     1141 ttcgaaaaaa tcagcaatgt aaacaggtat ctgcctggat aaccctgttt caccaac
//
LOCUS       DAOQWT010000534         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_156822_length_1190_cov_3.109251, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000534 DAOQWT010000000
VERSION     DAOQWT010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156822_length_1190_cov_3.109251"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="PKJ95_08500"
     CDS             complement(<1..1151)
                     /locus_tag="PKJ95_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNR66314.1"
                     /translation="MDIYETVVTITKPRMELKSYLDNIFMHPVLGYISLIAILYLFFN
                     FVFYLGNLLEEPLLGFFYQFLPLIEEKLGHNLLLFNIVSGIIQGIAGGIAIVLPYLFP
                     FLLGLAFLEDVGYLPRIAFLMDTFLHRIGLHGKAIIPLILGYGCTVPAVMATRILESE
                     RDRLIASILTTMIPCAARITIIFGLVAFYLGPKAALFVYILNIIVVAISGKILSQMMP
                     EITPGMIMEIPSYHIPSPKVLLAKVWLRMKEFIVIAWPLLIIGSIALSLLKYWKLEYF
                     INELISPVTLLLGLPIAVGVTLIFGILRKELSMIMLVQALGVTDISLVMNAAQIMTFT
                     IFVLFYVPCAATIAVLIREIGGEKTVFTVLFTFLIALILATATRLVYYV"
BASE COUNT          454 a          234 c          212 g          290 t
ORIGIN      
        1 aaaacataat aaaccagacg ggtagcagta gccagtatta aggcaataag gaaagtgaat
       61 aaaacagtaa acacagtttt ttccccgcca atctctctga ttaacacggc aatggttgcc
      121 gcacagggga catagaaaag cacgaaaata gtaaaagtca taatttgagc agcattcata
      181 accagactga tatcagtaac ccccaatgcc tgaaccagca ttatcataga gagttctttt
      241 cttaaaatcc caaaaatcag tgttaccccc actgcaattg gtaaacccag caaaagagtt
      301 accggggata tcagttcatt aataaaatat tctaacttcc aatattttaa caggctcaaa
      361 gctatgctac ctataattaa taagggccag gcaattacaa taaattcttt cattctgagc
      421 caaactttag ccaataatac ttttggtgat gggatatgat aagagggaat ctccattatc
      481 attccaggag ttatctccgg catcatctga gaaagtattt taccagatat agcaacaaca
      541 ataatattta aaatataaac aaacaaagct gctttaggac caagatagaa ggctaccaga
      601 ccaaatataa tagtgattcg ggcggcacag ggtatcatgg tagttaaaat actggctatc
      661 aaacggtccc gttcagactc taaaatacgg gttgccatta ccgcaggaac agtacaaccg
      721 tagcccagta taagtggaat aatcgcctta ccatgaagtc ctatccggtg taaaaaagta
      781 tccattaaaa atgcaattcg gggcaaatat cccacatctt ccaaaaaagc aagtcctagt
      841 aaaaatggaa aaaggtaggg caggactatg gcaattccac cagcaattcc ctgaataata
      901 ccgctaacta tattaaataa tagaagatta tggcctagct tctcttcaat gagaggaaga
      961 aattggtaaa agaaacccag caaaggctct tccaacaggt tccctaaata aaaaacaaaa
     1021 ttaaaaaaga gatatagaat ggctatcaaa gaaatataac caagaacagg atgcataaaa
     1081 atattatcca gataactttt tagttccatc ctgggcttag tgatagtcac tacagtttca
     1141 tagatatcca tagaaaggtg gtggcgttca gaggaaataa ccacatcaga
//
LOCUS       DAOQWT010000535         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_157651_length_1187_cov_1.948763, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000535 DAOQWT010000000
VERSION     DAOQWT010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157651_length_1187_cov_1.948763"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..645
                     /gene="nuoE"
                     /locus_tag="PKJ95_08505"
     CDS             148..645
                     /gene="nuoE"
                     /locus_tag="PKJ95_08505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNR66315.1"
                     /translation="MNETTVIKNEELTAEEKYQELGNFIRENREKRGYLIPVLYKAQS
                     LFGYLPKEVQKFIADEMNISVSEVFGVVTFYSYFKTQPVGRHTITVCMGTACYVRGAK
                     KILEALEDKLGIKIGETTEDLRFTLGEQRCFGSCGMAPVIMIDQDVHGRLTASKLDAI
                     LEKYK"
     gene            677..>1187
                     /locus_tag="PKJ95_08510"
     CDS             677..>1187
                     /locus_tag="PKJ95_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNR66316.1"
                     /translation="MNNIRTQLLLCHGTSCMSNGAARVREALEEALKEANLEKEVEII
                     HTGCMGICELGPIMVVYPDGVFYQQLKPEDAREIVQEHILKGRVVQRLFYKKPKTEEL
                     VAMMEDIDFFKLQRKIARRNCGIIDVNTIDDYIGADGFMALAKVLTEMTPQQVIDEIK
                     KSGLRGRGGA"
BASE COUNT          423 a          154 c          279 g          331 t
ORIGIN      
        1 ggagcctatg attcatatct atattgagga gcaaagaccg gtaacctatc gaaatgttga
       61 tataaatgcg gcaaggttaa tttttaacga acatgtttta aacaatagag tagtagaaca
      121 atatctattt tcaaagggga aaaaataatg aatgaaacaa ctgttattaa aaatgaagaa
      181 ttgaccgctg aagaaaaata tcaggaattg ggtaacttta ttagggaaaa tagagaaaaa
      241 agaggctatt taatcccagt tttgtataaa gcgcaatctt tgtttggtta cttacctaaa
      301 gaagtccaaa aattcattgc ggatgagatg aatatctcgg taagtgaagt atttggagtg
      361 gtaacattct attcctattt taaaacacaa cctgtaggaa ggcatactat tactgtctgt
      421 atgggaacag cctgttatgt gaggggtgct aaaaagattt tagaagcttt ggaagataag
      481 ttaggaataa agataggtga aacaacagaa gatctgaggt ttaccttagg agaacagcgt
      541 tgttttggct cctgtggaat ggcacctgtc attatgattg accaggatgt tcatggtcgg
      601 ttaactgcca gcaaattaga tgcaatatta gaaaaatata aatagttaaa taaggaaaat
      661 aaggagtaga ttaattatga ataatataag aacacagctt cttctgtgtc atggtacttc
      721 ctgtatgtct aatggagcag cgagagtgag agaagcattg gaagaggcat taaaagaggc
      781 taatctggaa aaagaagtag aaataataca taccggttgc atgggtatct gtgaactggg
      841 acctattatg gtggtctatc ccgatggtgt gttttaccag caattaaaac cagaagatgc
      901 ccgggaaatt gtacaggaac atattttaaa gggaagagtg gtacagcggc ttttttataa
      961 aaagcctaaa acagaagaat tagtggctat gatggaagac attgatttct ttaaattgca
     1021 gagaaaaatt gcccgcagaa actgtggaat catcgatgtt aacactattg atgattatat
     1081 cggggcagat ggttttatgg ctttagccaa ggtattgaca gagatgaccc ctcagcaggt
     1141 tattgacgaa attaaaaaat caggattaag gggcagaggt ggagctg
//
LOCUS       DAOQWT010000536         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_157967_length_1185_cov_3.051327, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000536 DAOQWT010000000
VERSION     DAOQWT010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157967_length_1185_cov_3.051327"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1185)
                     /locus_tag="PKJ95_08515"
     CDS             complement(<1..>1185)
                     /locus_tag="PKJ95_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNR66317.1"
                     /translation="EVVKKYKELVYKEIGEKFPEDPQKQLRMAIEAVFSSWDNKRAVT
                     YRRIHRIPDDLGTAVNVQEMVFGNMGTDSGTGVGFTRNPSTGEKENYGEYLLNAQGED
                     VVAGIRTPKSLTQLEKEMPDVYKQLIEKVKILENNYKDVQDYEFTIEKNKLYILQTRD
                     GKRTAQAALKIAIDMVKEGLIDKNTALKRIDPNQLNQLLHRRIDPNIELEPIATGLPA
                     SPGAASGKVIFTADEAEKRGKNGEAVILVRQETTPDDIHGMVQAKGILTSRGGMTSHA
                     AVVARGMGMPCVAGSNDIIINLEKELFKVNDKIIKKDDYITIDGGSGHVFMGKVPMIE
                     PKMSKEFKLLLSWADNIRKLGVYANADTPEDSKKARDLGAEGIGLCRTEHMFMAPERL
                     PIV"
BASE COUNT          296 a          279 c          185 g          425 t
ORIGIN      
        1 acaataggta atctctcagg agccataaac atatgttctg tcctgcatag acctatccct
       61 tctgccccca aatctctcgc ttttttggaa tcttcgggag tatcagcatt ggcatataca
      121 cctaattttc taatgttatc ggcccaggaa agcagtagtt taaattcttt actcattttt
      181 ggctctatca taggaacctt gcccataaaa acgtgaccgc tacctccatc aatagtaata
      241 taatcatctt tttttattat tttatcgttg actttgaata actccttttc caaatttata
      301 atgatgtcat tgctgccagc tacacagggc atgcccattc cccttgctac tactgcagcg
      361 tgactggtca ttcctcctct actggttaaa atcccctttg cctggaccat tccatgaata
      421 tcatcaggag tggtttcctg tctgaccagg atgacagctt caccattttt gcctcttttt
      481 tcagcctcat cagccgtaaa gataacctta cctgatgctg caccaggtga cgctggtaaa
      541 ccagtagcta ttggttctaa ctctatattg gggtcaattc tacggtgaag gagctgattt
      601 aattgattgg ggtctatccg ttttaaagca gtatttttat cgataagacc ttctttcacc
      661 atgtcaattg caatttttaa agcagcctga gcagttctct ttccatctcg cgtttggaga
      721 atgtaaagct tatttttctc tatagtaaat tcgtaatcct ggacatcttt ataattattc
      781 tctaatattt ttaccttttc aatcaattgc ttataaacat caggcatttc tttttccaat
      841 tgggtaagag attttggcgt tcttattcca gctacaacat cttctccctg agcatttaaa
      901 aggtattctc cataattttc tttctctcca gttgaagggt tacgtgtgaa accgactcct
      961 gtccctgaat cagttcccat gttcccaaaa accatctcct gaacattaac cgcagtaccc
     1021 aggtcatctg gaattctatg aattcttcta taggtaactg cccttttatt atcccaggaa
     1081 ctgaaaacag cttctatggc catccttaat tgtttttgtg ggtcctccgg gaatttttcc
     1141 cctatttctt tatagactaa ttctttatat ttcttaacta cctct
//
LOCUS       DAOQWT010000537         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_159326_length_1179_cov_3.239324, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000537 DAOQWT010000000
VERSION     DAOQWT010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159326_length_1179_cov_3.239324"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..>1179)
                     /gene="rho"
                     /locus_tag="PKJ95_08520"
     CDS             complement(248..>1179)
                     /gene="rho"
                     /locus_tag="PKJ95_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNR66318.1"
                     /translation="FALLKVEAVNHEDPELSKERIDFENLTPLFPMERFKLETKPYEL
                     STRVIDLFSPIGKGQRGLIVSPPKAGKTMLLEKIANGIITNHPETILMILLIDERPEE
                     VTNMRRSVLKAEVISSTFDQPINNHIKVAEIVLERAKRLVELKHDVVILLDSITRLAR
                     AYNLNIPTSGKTLSGGLDSTALYFPKRFFGAARNIEEGGSLTILATALVETGSRMDDV
                     IFEEFKGTGNMELRLDREISDNRIFPAIDIKRSGTRKEELLLDADELGKVWLMRRALS
                     NQSTIDSISLLLNKLKKTKNNHDFLNSIKDENH"
BASE COUNT          344 a          227 c          204 g          404 t
ORIGIN      
        1 cttttttgga ggtattgtaa ttataaaaac agtggtctgt tttaataaaa tataaacttg
       61 ttttgctatg ccctattttt ggaaaattaa atttaccagg attttagttt ctatcaatta
      121 tgatgctaaa ataacgatga aattatagca caagtatttt atagcgtcaa agacaataaa
      181 tatttttata tttttcgttt tattcagcta aatcttcctg gtaaatggct taaagatggt
      241 agcagtttta atggttttca tctttaatgg aattgagaaa atcatgattg tttttagtct
      301 tttttaattt gttcaggagc aggcttatgg aatcaatggt gctctgattg gataatgctc
      361 tcctcattaa ccagacttta cccagctcat ctgcatctaa taataactct tcctttctag
      421 tgccggaacg ctttatatca atagccggga aaattcggtt atcagatatt tcccgatcta
      481 agcgtaattc catattaccg gtacctttaa attcttcaaa tattacatca tccattcgac
      541 taccagtttc aacaagggct gtggccagga tagtcaaact tcccccctct tcaatatttc
      601 tggctgctcc aaaaaatctt ttggggaaat atagcgcagt tgaatctaat cctcccgaca
      661 gggttttacc actggtagga atgttcaggt tataagccct ggccagcctg gttatactgt
      721 ccagtagaat aaccacatca tgtttaagtt ctactaatct ttttgccctt tccagaacaa
      781 tttcagcaac tttaatatga ttgttaatag gctggtcaaa agtagaacta ataacctcag
      841 cctttaacac agaacgcctc atattggtaa cctcttccgg cctctcatca atcaacagga
      901 tcataagtat cgtttcagga tgattggtaa tgataccatt ggcaatcttc tccaggagca
      961 ttgtcttacc ggccttggga ggagatacta ttaatcccct ctgtcccttt ccaatgggtg
     1021 aaaagagatc aattaccctg gtagacaatt catagggctt ggtttctaat ttaaatcttt
     1081 ccatggggaa aagcggtgta agattctcaa aatcaatacg ttctttagat aattcgggat
     1141 cttcgtgatt aactgcttct accttgagta aggcaaagt
//
LOCUS       DAOQWT010000538         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_160896_length_1172_cov_2.785139, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000538 DAOQWT010000000
VERSION     DAOQWT010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160896_length_1172_cov_2.785139"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..>1172
                     /locus_tag="PKJ95_08525"
     CDS             78..>1172
                     /locus_tag="PKJ95_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HNR66319.1"
                     /translation="MNQYRKSFLLVLTLILLLSLTTSSALGQGKWKIGIMTGTVTQGE
                     EEFRAAQEVLAKYGPEHVILMTYPDKFMDEQETTIANMVSMASDPDVGALIICQAVPG
                     TSPGIDAVKELRDDLLILTAVQHEDPPVISARADLVLASDELGMGYTIPAQAKKLGAK
                     VLVHYSFPRHMSYPILSGRREILKTECARLGLEFVDATSPDPTGDAGISGSQQFILED
                     VPRMVEKYGKDTCLFSTNCSQQIPLITAVVATGAIYAQPCCPSPLHGFPSALGIEIPE
                     DKQGNIPYVVEEITKIMAAKGMTGRTSTWPVPVAMMFINGLSEYAKSWILGEFTEKMN
                     IPVLTQKLADYAGVDITLNPLVEGGVTYDNF"
BASE COUNT          345 a          244 c          254 g          329 t
ORIGIN      
        1 acagcaaaaa ttaagtttta ctgctattcg tcttatgata gtcagatttt aaaaaaaaat
       61 aaatttggag gaaaaaaatg aatcaatacc gtaaaagttt tctattagtt ttaacattaa
      121 ttcttttatt atcgttgaca acctcttcag cattgggaca gggtaaatgg aaaattggta
      181 taatgaccgg cacagtaact cagggagaag aagagttcag agcagctcag gaagtacttg
      241 ccaaatatgg tcccgaacat gtcattttaa tgacctatcc ggataaattt atggatgaac
      301 aggagaccac cattgccaat atggttagta tggcttcaga tcctgatgta ggagcattaa
      361 taatctgtca ggcagttcct ggaacctcac ccggtataga tgccgtaaag gagctcaggg
      421 atgaccttct gattttaaca gcggtacaac acgaagaccc gccggttatt tcagccaggg
      481 ctgacctggt tttagcctcg gatgaattgg gaatgggcta caccattcct gctcaagcca
      541 agaaattggg agccaaagta cttgttcact attccttccc cagacatatg tcttatccca
      601 ttctatcggg aagaagagaa attttaaaga cagaatgtgc ccgccttggt ttggaatttg
      661 ttgatgctac ctctccggat ccgaccggtg atgccggtat ttctggttca caacaattta
      721 ttctggaaga cgttcccaga atggtagaaa agtatggtaa agatacctgc ctcttcagca
      781 ccaactgctc acagcagata cctttaatca ctgctgtggt ggcaacggga gcaatttatg
      841 cacaaccctg ctgcccctca cctcttcatg gtttcccctc tgctctgggc atagaaattc
      901 ctgaagacaa acagggaaat attccttacg tggtagaaga aattaccaag attatggctg
      961 caaaagggat gaccggaaga acctctacct ggccggttcc tgttgctatg atgtttatta
     1021 acgggttatc tgaatatgct aaatcctgga ttttaggtga gttcacagaa aaaatgaata
     1081 ttcctgttct aacccagaaa cttgctgatt atgccggtgt tgatatcacc ctgaatcctc
     1141 tggtagaagg cggggttact tacgataact tc
//
LOCUS       DAOQWT010000539         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_160899_length_1172_cov_2.737690, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000539 DAOQWT010000000
VERSION     DAOQWT010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160899_length_1172_cov_2.737690"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /gene="tmk"
                     /locus_tag="PKJ95_08530"
     CDS             <1..622
                     /gene="tmk"
                     /locus_tag="PKJ95_08530"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNR66320.1"
                     /translation="YKILSKNGLKAVLAKEPGGTIIGDKIRKIVLERGNEELGYKAEL
                     LLFAASRAENVRINIKPSLEKGFIVICDRYFDSTTAYQGFGRGIDMNIIECINSFVVE
                     DIIPDLTLFLDVDSQVGLQRATIYNKNLELRFEDEFLQKKEVDGKLFLERVRDGYYQI
                     ARQNPDRIKIIDANRDISLLIKDIIEVLNFRLSQKYKKEIILKLPN"
     gene            633..>1172
                     /gene="holB"
                     /locus_tag="PKJ95_08535"
     CDS             633..>1172
                     /gene="holB"
                     /locus_tag="PKJ95_08535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNR66321.1"
                     /translation="MVIILNIIDFEKIVGQERALQLLKNIIKSDQIGHAYLFSGKEGI
                     GKRITAIAFARAINCTNLSDDCNPCNSCSSCLKIEKGIHPDFQIISPINSVITIGQIK
                     NIKEIIYWKPLTGRKKIFLVDDAHKMTIEASNSLLKILEEPPEFVVFILITHSPGFIL
                     PTIVSRCHQISFQPISATKQ"
BASE COUNT          401 a          190 c          226 g          355 t
ORIGIN      
        1 gtacaaaata ttatcaaaaa atggattaaa ggcagtttta gccaaagaac ccggaggtac
       61 cattattggt gataaaattc gtaaaatagt actggaacgg ggaaacgaag aattaggtta
      121 taaggcagaa ttattgttgt ttgctgcctc gagggctgaa aatgtcagaa tcaacatcaa
      181 accctccctg gagaaaggat ttattgtcat ttgtgaccgc tattttgatt ctaccactgc
      241 ctatcagggt tttggcagag gtatagatat gaacataatt gaatgtataa atagttttgt
      301 ggttgaggat attatcccgg atttgaccct ctttttagat gttgacagcc aggtcggatt
      361 acagcgggct actatatata ataagaattt agaactgcgt tttgaagatg aatttcttca
      421 aaaaaaagag gtagatggaa aattattcct ggaaagagtg agggatggat actaccagat
      481 tgccaggcag aatcctgacc gaattaagat aattgatgcc aaccgtgata tctctctgtt
      541 aataaaagac attatagaag tgttgaattt caggctctcg cagaaatata aaaaagaaat
      601 tatcttaaag cttcctaatt gaaataaggt atttggttat tattttgaat ataattgatt
      661 tcgaaaagat tgttggacag gaaagggctc ttcagctctt aaaaaatata ataaagtctg
      721 atcagatagg tcatgcctat ctttttagcg gtaaagaagg gattggaaaa agaattaccg
      781 ctatagcctt tgccagggct ataaactgta ctaatctatc tgatgattgt aacccctgca
      841 acagctgttc ctcctgcctg aaaattgaaa agggcataca tcccgatttt caaattattt
      901 ctcccattaa ttctgttatt accataggtc aaattaaaaa tataaaggaa ataatctatt
      961 ggaaaccctt gacaggcaga aaaaaaatat ttttagtcga tgatgcacat aaaatgacca
     1021 tagaagcttc aaattcctta ttgaagatat tggaagaacc gcctgaattc gtagttttta
     1081 tcttaattac tcattcaccc gggtttattc tacccactat tgtctccagg tgtcaccaga
     1141 tatcttttca accgataagc gcgacaaaac aa
//
LOCUS       DAOQWT010000540         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_161134_length_1171_cov_2.643369, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000540 DAOQWT010000000
VERSION     DAOQWT010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161134_length_1171_cov_2.643369"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKJ95_08540"
     CDS             complement(<1..211)
                     /locus_tag="PKJ95_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66322.1"
                     /translation="MNKVSFPNDPEDDTDNTKYNAESVIRLITCLIFIWFIKEKKLIP
                     ENIFDKNELSKILKGFGEKDSTVYYR"
     gene            complement(335..952)
                     /locus_tag="PKJ95_08545"
     CDS             complement(335..952)
                     /locus_tag="PKJ95_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66323.1"
                     /translation="MAQSEQEKKYLIKSAIGNFSQNNLTSQSIHLFQTLGYNTRVQDP
                     FLEKDYKQFHYNYSEYFLEKGFNEEKALTKEWKYIDLLFQLTKNELSAQNNLFHTGKV
                     KWQGEDKETVIETYLFFAIGLRKNEYSRTELAQITREINKLFPMPVMLIFKYGNKLTL
                     AIINRRLHKKDSQKDVLEKVTLIKDISISTPHRAHIEILFDLSLE"
     gene            complement(957..>1171)
                     /locus_tag="PKJ95_08550"
     CDS             complement(957..>1171)
                     /locus_tag="PKJ95_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66324.1"
                     /translation="CTLFNDKTCNGENMEHYTDLLKKAINEIVQIFKKRSHRSLITDR
                     AAQIIPQAKQISEMEDFELITWLVLE"
BASE COUNT          333 a          191 c          200 g          447 t
ORIGIN      
        1 cccgataata gaccgtagaa tccttttctc caaacccttt tagaatttta gaaagttcat
       61 ttttatcaaa gatgttctca ggaatcaatt ttttttcttt aataaaccag ataaagataa
      121 ggcaggtaat cagtctgatg acactttcgg cattgtattt agtattatcg gtatcgtctt
      181 cagggtcatt ggggaaagat actttgttca ttgcccagaa ataccagttt gaaagctcac
      241 gatagaattt tttgtttaat tctttggtat ctaaggtttc ctgccaggca ttgtgcagct
      301 caacaaagtt agtaaatcta tattttcttc ttaattattc taaagatagg tcaaagagta
      361 tctctatatg tgcccgatgt ggggtggata tagagatatc tttaatcagg gtgacttttt
      421 ccaatacatc tttttggcta tcttttttgt gtaatctacg gttgatgata gccaaagtga
      481 gcttattacc atatttgaaa attagcataa ccggcatggg gaagagttta tttatttcac
      541 gggttatttg ggctaattct gtacgggaat attcattttt ccttaatcca atcgcaaaga
      601 aaaggtatgt ttcaatgacg gtctctttat cttcaccctg ccattttact ttaccagtat
      661 gaaaaagatt attttgggca gatagttcat ttttagttaa ttgaaataat aggtcaatgt
      721 atttccattc cttggttaga gccttttctt cgttaaatcc tttttctaaa aaatattcac
      781 tataattata atgaaactgc ttataatctt tctccagaaa agggtcttgc actctagtgt
      841 tatagccgag ggtttggaat agatgaatcg attgggaggt aagattgttt tgtgaaaagt
      901 ttccaatagc agatttgatt aaatattttt tctcttgttc tgattgtgcc atatatttac
      961 tccaatacta accaggtaat caattcaaaa tcttccattt cgctaatctg tttagcctgt
     1021 ggaattattt gtgctgcccg gtctgtaatt aaacttctgt gacttctctt tttaaatatt
     1081 tgaacaatct cattgattgc ttttttcaac aaatctgtat aatgctccat attttcccca
     1141 ttgcaagtct tatcgttaaa tagggtacag a
//
LOCUS       DAOQWT010000541         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_161588_length_1169_cov_3.036804, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000541 DAOQWT010000000
VERSION     DAOQWT010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161588_length_1169_cov_3.036804"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PKJ95_08555"
     CDS             <1..666
                     /locus_tag="PKJ95_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNR66325.1"
                     /translation="TKDVFKLKNGNYLLKFKDDVTGENGIFDPGANTVGLSIAGAGRA
                     ALKLTKFFFEKLEKNNIPTHYIEANLEQDAMIVKPATVFGKGLEVICRYRAVGSFFRR
                     YGEYIAEGSPLDALIEFTLKDDARNDPLITEDALSMLKILSQEECKELKYLTRRIAGL
                     IKEELSKKGIELYDIKLEFGREKDSGELILIDEISGGNMRAYKDGKYLQPLELGKLIL
                     SDI"
     gene            713..>1169
                     /locus_tag="PKJ95_08560"
     CDS             713..>1169
                     /locus_tag="PKJ95_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNR66326.1"
                     /translation="MEKTLVLIKPEAIKRALVGRIISRFERAGLNLREMKIIKPSREI
                     VEKHYPDDCHWVKNVGKKTIDTYKRYNLNLKQDLGTEDPLEIGQIIRKWLMEHLTDNP
                     VVVIILSGNHAAEVTRKMVGNTVPLFAELGSIRGDFSIDSPDCSTPEKRV"
BASE COUNT          429 a          161 c          254 g          325 t
ORIGIN      
        1 acaaaagatg tttttaagct aaaaaacggt aattacctct taaaatttaa ggatgatgta
       61 accggagaaa atggtatttt tgaccctggc gcgaatactg ttggcttaag catagctgga
      121 gcaggaagag ctgccctaaa attaaccaag tttttttttg aaaaattgga aaagaacaat
      181 atacctacac actatattga ggcaaatctc gaacaggatg ctatgatagt aaaaccggct
      241 actgtgtttg gaaaaggatt ggaagtgata tgccgctata gagctgtggg cagttttttc
      301 cgtcgttatg gagaatatat tgcagaaggc agtcccttag atgcattaat tgaatttacc
      361 ttaaaagatg atgccagaaa tgatcctttg attactgagg atgccctgtc aatgcttaag
      421 atattatccc aggaagagtg taaagagtta aaatatctta ccaggagaat tgctggattg
      481 ataaaagaag agttatccaa aaaaggaata gagttatatg atattaaact agaatttggt
      541 agagagaagg atagcggaga attaattttg attgatgaga tatctggagg caatatgaga
      601 gcttataaag atggaaaata tcttcaacca ttagaattgg ggaaattgat tttatctgat
      661 atttagaaat agttcataag caattaaata aaataagaaa ggattattag aattggaaaa
      721 aactctggtt ttgattaagc ctgaagcgat aaaaagggct ctggtaggca ggataatatc
      781 ccgctttgaa agagcaggtc ttaatttaag agagatgaaa attataaagc catcaaggga
      841 aatagtagaa aaacactatc cggatgattg tcattgggtc aaaaatgtgg gcaaaaaaac
      901 aatagatacc tacaagagat ataatttaaa tttaaaacag gatttgggaa cagaagaccc
      961 actcgaaatt ggtcagataa tccgaaaatg gttaatggag cacctgactg ataatcctgt
     1021 agtagttatt atcttaagcg gcaaccatgc cgctgaggtc accagaaaaa tggtaggtaa
     1081 tacagtcccc ctttttgcag agttaggttc tataagaggt gatttttcca tagattcccc
     1141 cgattgctct acaccagaaa aaagagttt
//
LOCUS       DAOQWT010000542         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_162024_length_1167_cov_4.799460, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000542 DAOQWT010000000
VERSION     DAOQWT010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162024_length_1167_cov_4.799460"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PKJ95_08565"
     CDS             complement(<1..1156)
                     /locus_tag="PKJ95_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR66327.1"
                     /translation="MCILNFIPGDSLGTYNYELQISSNDPVSPLITVPLEYIVTSPIA
                     YIPDINFRMAINEALGQPSDYQPTIADLNGLTGILYAHDRYIISIVGAQYLTNLQELY
                     LNNNQISDLSPLAGLTNLKRLYLSSNQISDLSPLMGLNNLKWLHLDNNQISDLSPLAG
                     LTNLQLLTLGENQISDLSPLAGLTNLYSLNISVNQISDLSPLAMLTNLDHLEIFNNQL
                     SDLSPLAGLNTLQGLLLQNNQISDISPLAGITNLQVLYLNYNQISDLNPLVDLTNLQN
                     LRFDYNQISDLSPLAGLTNLQWLYFANNQISDLGPLAGLINLQVLVLWNNPISYESML
                     LSQSWSLPCHISTYNSLSPCYPDPSRNATNVLVNYPLSWQGNYDHSQDVFY"
BASE COUNT          397 a          211 c          233 g          326 t
ORIGIN      
        1 catagaaaac atcctggcta tgatcataat tcccctgcca ggataaagga taatttacca
       61 aaacattggt cgcatttcta ctgggatcgg gataacaagg cgaaagggaa ttgtaagttg
      121 aaatatgaca aggtaaagac caactctgag atagcagcat tgactcataa gagataggat
      181 tattccaaag taccagcact tgcagattaa taagacctgc taaagggccg aggtcactta
      241 tctggttatt agcaaaatat aaccattgca gattagtcag acctgctaaa ggactgaggt
      301 cacttatttg gttataatca aaccttagat tttgcagatt agtcagatca actaaaggat
      361 tgaggtcact tatctggtta taattaagat acagcacttg cagattagtt atccccgcta
      421 aaggacttat gtcacttatc tggttatttt gaagaagcag cccttgcaaa gtattcagac
      481 ccgctaaagg actaaggtca cttaactggt tattaaaaat ctccagatga tccaaattag
      541 tcaacatagc taaaggactg aggtcactta tctggttaac actaatattc agagaataca
      601 gattagtcag ccccgctaaa ggactgaggt cacttatctg gttttcacca agagtcagca
      661 attgcagatt agtcagcccc gctaaaggac tgaggtcact tatctggtta ttatcaagat
      721 gtagccattt cagattattc agacccatta aaggactgag gtcacttatc tggttagaac
      781 taagatacaa ccgtttcaga ttagtcagac cagctaaagg actgaggtca cttatctggt
      841 tattattaag ataaagctct tgcagattag tcagatattg agcacctact atactaatta
      901 tataccgatc atgagcatat aaaattccag ttaatccatt caaatcagca atagtaggtt
      961 gataatcaga gggctgacct aatgcctcat ttattgccat tctaaaattg atatcaggaa
     1021 tataggcaat aggactggta actatatatt ctaaaggaac agtgatcaga ggtgaaacag
     1081 gatcattgga tgagatctgc agttcatagt tgtaggtgcc aagggaatct ccaggaataa
     1141 aattcaggat acagattact gaagaat
//
LOCUS       DAOQWT010000543         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_162050_length_1167_cov_3.190647, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000543 DAOQWT010000000
VERSION     DAOQWT010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162050_length_1167_cov_3.190647"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            485..1111
                     /locus_tag="PKJ95_08570"
     CDS             485..1111
                     /locus_tag="PKJ95_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNR66328.1"
                     /translation="MTTGKQRINYTTIAILLVSAAFITIFFVISTPAIAQYKTGSPAP
                     DFTLEALDGEIFQLNQFLNKQQHLILFFMHSEEDSSLAKLGDLITFLNDYQPRESYQI
                     VAIMEQSQDSEGAIEKLNNLKSKAEIPLIILLDTDGKINTVYGVKTYPTVLLLRTDLY
                     VRKAYDRFTSRQEASFYQYLKFIFTSQKSKDSGNGCDDGVYPPPPGFE"
BASE COUNT          391 a          202 c          187 g          387 t
ORIGIN      
        1 attcaaggca acccggttcg aaaaaatcag caatgtagac aggtatctgt ctggataaca
       61 atgttttacc aacttcctgt cctcttatag aaataaaaaa catatttaaa acaacaatca
      121 aaaataatat tgttaaaaaa aacgtaacgc cagcacatct gctcttttcc actgaaaaca
      181 ttttattttg ataatctccc ttcattcaat tttctatttt aaaaagaaat tatatggaac
      241 atgttttgta ttatacctat tccatccctt aatttcaaaa aaagtgggat taccttagcc
      301 tttctcatta tcttttacgg taaaaagggc gcctaaaata gctctttcat aaactgattt
      361 gttgtttaaa taatttgttt ttattaaatt agatttgctt ccagctcagt ttttagttag
      421 aattatggta gaaatatagg attattaaag agcaaatcta tcattgagat ataaggagtt
      481 tgatatgact acaggaaaac aaagaattaa ttatactact attgccattt tgttggtatc
      541 agcggctttt ataactatct tttttgttat ctctactccg gcaattgccc aatacaagac
      601 cggctcgccg gctcctgatt ttaccctgga agcattggat ggggagattt tccaactaaa
      661 tcaattttta aataaacagc agcatctaat actcttcttt atgcatagtg aagaagattc
      721 ctccttagct aaattaggtg atttaattac ctttttaaat gattaccagc cccgagaaag
      781 ttatcagatt gttgccatta tggaacagag tcaggatagt gagggagcta tagaaaaatt
      841 gaataacctt aaaagtaagg cagaaatacc tttaattatt ctgctcgata cagatggtaa
      901 aatcaatact gtctatggag tcaaaaccta tcctactgtg cttttactga gaactgattt
      961 atatgttagg aaagcatatg accgcttcac ttccaggcag gaagccagtt tctatcaata
     1021 tctgaaattc atctttacct ctcaaaagag caaagattct ggaaatggtt gtgatgacgg
     1081 tgtctacccc ccaccgccgg gatttgaata atagaatagg gggtgaagaa aatgtattcc
     1141 ttcagaaata aaatattttt catcttt
//
LOCUS       DAOQWT010000544         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_162341_length_1166_cov_2.337534, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000544 DAOQWT010000000
VERSION     DAOQWT010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162341_length_1166_cov_2.337534"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..>1166
                     /locus_tag="PKJ95_08575"
     CDS             187..>1166
                     /locus_tag="PKJ95_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNR66329.1"
                     /translation="MRERELVMDFKTTLNLPKTDFSMKANLPKEEPNTLKFWQEIDLN
                     KKLLNKYRDKEKFILHDGPPYANGNIHIGTAYNKILKDIIPKFKWMRGFNSYYVPGWD
                     THGMPIEHQVSRDLKVSLNEINPVVLREKCQKYAEKYIDIQREEFKRLGVIGDWENPY
                     LTFLPEYEATQIGIFGKMYERGYIYKGLKAVYWCGSCETALAAAEIEYSDESSDSIYV
                     KFEVKDDISSIFPEAKPGDTYVLIWTTTPWTIPGNFAIAFNPDLKYSLVKTERGYLIL
                     ASGLVEKVMKEIGVDDYKVLAEQVGSVFEGIKCRHPLLDRDSVIILGKHVT"
BASE COUNT          390 a          178 c          256 g          342 t
ORIGIN      
        1 agcatattta ccgaaaatcg cctctcaatt ctgactgaaa acggacagta agtcaagacg
       61 gttgcaccgt tagagcaggg taaggaaaaa tattaagtat ttttccttat gagtttataa
      121 attaaggtgg taccgcagga agtgttaacc tcttgccctt taccggcagg aggttttatt
      181 ttataagtga gggaaaggga actggttatg gattttaaaa caacattaaa tctaccaaaa
      241 actgattttt caatgaaagc taatctgcca aaagaagagc ctaatacact taaattctgg
      301 caggaaatag atttgaacaa aaaattattg aataaatatc gggataaaga aaaatttatc
      361 ctccatgatg ggccacctta tgctaatgga aatattcata ttgggactgc ctataataaa
      421 attttaaaag atatcattcc aaagtttaaa tggatgaggg gttttaattc ttattatgtt
      481 ccagggtggg atacccatgg tatgcctatt gaacaccagg taagccggga ccttaaggta
      541 tctcttaatg agatcaatcc ggtggtattg agggaaaaat gtcagaaata tgccgaaaaa
      601 tacattgata ttcaaagaga agaatttaaa agacttggag ttattggaga ttgggaaaat
      661 ccttacttaa cctttttgcc tgaatatgaa gctacccaga taggcatatt tggaaaaatg
      721 tatgaacgtg gatatattta taaagggttg aaggcggtat actggtgtgg ctcgtgtgaa
      781 actgccttag ctgcagctga gattgaatac agcgatgagt ccagtgactc tatttatgtc
      841 aagtttgagg ttaaagatga cataagctca atatttccag aagccaagcc tggagatact
      901 tatgtattaa tctggactac tactccctgg actattccag ggaacttcgc catagccttt
      961 aatcccgatt taaaatacag cctggtaaag acagagagag gttatctaat cctggcttct
     1021 ggtttggtag aaaaagtaat gaaagaaata ggagtggacg attataaggt tttggctgaa
     1081 caggttggca gtgtttttga aggtattaaa tgccggcacc ccttattgga tagagattca
     1141 gttatcattt tgggaaaaca tgtcac
//
LOCUS       DAOQWT010000545         1164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_162733_length_1164_cov_3.653742, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000545 DAOQWT010000000
VERSION     DAOQWT010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162733_length_1164_cov_3.653742"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKJ95_08580"
     CDS             <1..192
                     /locus_tag="PKJ95_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNR66330.1"
                     /translation="GSLLRSGATIMTISAFITTLVMVGLVTVPMEIKSLGKKFTFLRN
                     SFSLIGALIIAVLMGVILK"
     gene            189..701
                     /locus_tag="PKJ95_08585"
     CDS             189..701
                     /locus_tag="PKJ95_08585"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNR66331.1"
                     /translation="MTTLKQFRTPILLGIIYLILMVWLPGTAQRTIAVTISYAKEMAI
                     IMPAVFVLMGLIEVWIPGDKIQKWLGASSGVKGVLISFLMGTLPTGPLYVAFPLAASL
                     LKKGASITNTVIFLGSWAALKIPQLLVEIKFLGGTFTIVRFVLTLMVIIVMGIIMNIL
                     LKKDLENIQE"
     gene            822..>1164
                     /locus_tag="PKJ95_08590"
     CDS             822..>1164
                     /locus_tag="PKJ95_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66332.1"
                     /translation="MKKKVISLTLIIAVALMVFSTASFADARGHRNFAGKQGIPFHQG
                     QNQNPAERFGFNQGDNYRGMRNLELSSEQMAQIREMMLTFQKETLELRKQIQTKQLEL
                     REMRLSSDLDLG"
BASE COUNT          385 a          190 c          232 g          357 t
ORIGIN      
        1 ggttctttgc tacgttcagg agcaactatt atgactatat cagcctttat taccacactg
       61 gtcatggtcg gtctggtaac tgttcctatg gagataaaat cattaggcaa aaaatttaca
      121 ttcttacgca attctttcag cttaatagga gctttaatta ttgcagtatt gatgggggtg
      181 atcttaaaat gaccacttta aaacaattta gaacaccgat tttgctggga attatttacc
      241 ttattttaat ggtatggctt cccggtacag cacagagaac aattgcagta accatctctt
      301 atgccaaaga gatggccata ataatgcctg ctgtatttgt tttaatggga ttgatagagg
      361 tgtggattcc gggagataag atacagaaat ggctgggagc aagctccggc gtgaaaggag
      421 ttttgatatc ttttttaatg ggaacactcc ctaccggacc tttatatgtc gcctttcctt
      481 tagctgcttc tctattaaag aaaggggcaa gtattaccaa tactgtaata tttttaggtt
      541 cctgggctgc tctgaagata cctcagcttt tagtagaaat aaaatttctg gggggaactt
      601 ttaccatagt acggtttgta ttaactctaa tggtcataat tgttatggga attattatga
      661 atatattgct caaaaaagac ttagagaaca ttcaggaatg agccagtgtc agccttttta
      721 aaattaattt ataatttttg caatagttga aataaacaag ctcacttttg cgtatatata
      781 gatgatgaca aaaattttca taaaaagaag gagtaaaaat aatgaaaaag aaagttatct
      841 ctttaacact aataatagca gtagcattaa tggtatttag cacagcatca tttgctgatg
      901 cccgcggtca tagaaatttt gctggcaagc aaggcatacc ttttcatcaa ggacagaacc
      961 agaatcccgc agaacggttt ggcttcaatc aaggagataa ttacagagga atgagaaatc
     1021 ttgaactatc ttctgaacag atggctcaaa ttagagaaat gatgcttacc ttccagaaag
     1081 agactttaga actcagaaag cagattcaaa ccaaacaatt agaactaaga gagatgagac
     1141 tgtcatcaga tttagatctg ggaa
//
LOCUS       DAOQWT010000546         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_163056_length_1163_cov_2.320397, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000546 DAOQWT010000000
VERSION     DAOQWT010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163056_length_1163_cov_2.320397"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="PKJ95_08595"
     CDS             complement(<1..1135)
                     /locus_tag="PKJ95_08595"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNR66333.1"
                     /translation="MTESLLKELSLKTDSRIVLLVMDGLGDLPGKDGKTVLEQAEIPN
                     LDRLAAKSVCGLTDPISRGITPGSGPAHLSLFGYDPLKYQIGRGVLEALGIGLELTCD
                     DLACRGNFATIDENGVITDRRAGRISTEKNQELCSLLQKEITEIDGVKVIIRSGKEHR
                     FVVVFRGKGLEDGISDADPQLNGKKIKYAEALRDESKWSVQIINRFIDRCLEVLKGHH
                     PANAILLRGVAKHPELPSMGELFKLNPAAIASYPMYRGLARLVGMSILNTGEAIGDEF
                     NTLKENYHKYDFFYLHIKKTDSYGEDGNFEAKLKVVEEVDCYIPALLELNPEVLVVTG
                     DHSTPAALAAHSWHPNPFLLYSKYIRVDDVQHFSENEFVRGGLG"
BASE COUNT          316 a          286 c          211 g          350 t
ORIGIN      
        1 tccctaaacc gcccctgaca aattcgtttt cactgaaatg ctgcacatca tctaccctta
       61 tatattttga atagagaaga aaaggattag ggtgccagct atgagcagcc agggctgccg
      121 gtgtagaatg gtcaccggtt accaccagta cttccggatt tagctccagc aaagccggta
      181 tatagcaatc tacctcctca actactttaa gttttgcctc aaagtttcca tcttctccat
      241 aactgtcagt ctttttaata tggaggtaga agaaatcata tttatgataa ttttctttta
      301 aagtgttaaa ctcatcgcca attgcctcac cggtattcag aatactcatt ccaaccaatc
      361 tggctaaacc gcgatacata ggataagaag caatggcggc agggttaagt ttaaatagtt
      421 cacccataga aggtagttcg ggatgtttgg ctactcccct taaaagaatg gcattcgccg
      481 ggtgatgtcc ttttagaact tccagacatc tatctataaa acggttaatt atttggacac
      541 tccacttgct ctcatctctc aaggcttcag catattttat ctttttgcca ttgagctgcg
      601 ggtcagcatc agaaattccg tcttccaacc ctttacctct gaagaccacc acaaaacgat
      661 gctctttacc tgatctgatt atgactttga ccccatcaat ctctgttatc tctttttgta
      721 ataaagagca taattcctga tttttctctg tggagatgcg gccagctcta cggtcggtaa
      781 tcacaccatt ttcatcaata gtagcaaaat ttccccggca ggcaagatca tcacaggtaa
      841 gctctaaccc tattcccaat gcctctaaga caccccttcc aatctgatat ttaagagggt
      901 cataaccaaa tagagacagg tgtgccggtc cgctaccggg tgtaattccc cgggaaatag
      961 ggtcagtcaa accgcatact gatttggctg ccaatctatc gaggttgggt atttccgcct
     1021 gttccagtac tgtttttccg tctttgccgg gaaggtctcc taaaccatcc atgactaaaa
     1081 gaacaatcct ggaatctgtt tttaatgata gttccttcaa taagctttcg gtcatcaaag
     1141 ctatactctc ctttcactct aca
//
LOCUS       DAOQWT010000547         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_164221_length_1158_cov_2.588395, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000547 DAOQWT010000000
VERSION     DAOQWT010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164221_length_1158_cov_2.588395"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PKJ95_08600"
     CDS             complement(<1..1149)
                     /locus_tag="PKJ95_08600"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNR66334.1"
                     /translation="MSNSQELYLKGISAVFAISNAKEYIEGLALITHKLIEIMNSDII
                     FVLVNMRNRIYVVARSNRNSVNVGEILKTLGGGGHSQAASAVIRDMSLIDTENKIKQL
                     LEKHIQVETLAIDIMTTPVKTIDISTTIEEANKIMLRYGHNGFPVMNNGKLVGIITRQ
                     EIYKAKHHHYENETVDVYMSENIISVNPNTPILEIQELMIRYDVGRVLVLNNHGKLEG
                     IITRTDLIRSLHGDDGIRKESYSLFEKSREDYQYSRGKIKSLMYQYFNPEILSALLEI
                     GRIGDHFGYSIFMVGGIVRDLLLGIPNLDLDIVVEEDAIKFAHFFCEQVKGKIKIHQK
                     FKTAVVILPNGIKIDFASARREFYEFPAALPDVEFASIKKDLYRRDFTI"
BASE COUNT          355 a          223 c          156 g          424 t
ORIGIN      
        1 aatagtaaaa tctcttcgat ataagtcttt ttttattgag gcaaattcaa catcgggtaa
       61 agcagctggg aattcataga attctcttct ggcagaggca aagtcaattt ttattccgtt
      121 aggcaaaata acaaccgctg ttttgaattt ttgatgaatt ttaattttac cttttacttg
      181 ttcacaaaaa aaatgggcaa attttatagc atcttcttca actactatat caaggtctaa
      241 attaggaatg cccaatagta aatctcttac aattccgccc accataaaaa ttgaatatcc
      301 aaaatgatct cctattcttc caatttcaag caaagcggag agtatttccg ggttaaaata
      361 ctggtacatt aaggatttta tttttcctct actatactga taatcttccc tgcttttttc
      421 aaatagagag taggactctt ttctaatacc atcatcccca tgtaaacttc ttattaggtc
      481 tgtcctggta ataattcctt ctaattttcc atgattattc aaaactaaaa ctctgccaac
      541 atcatacctg atcattagct cctgtatttc taatataggt gtattgggat ttacactgat
      601 aatgttttca gacatataga catcaacggt ttcattttca taatggtgat gttttgcctt
      661 atagatttcc tgcctggtaa ttatacctac taattttcca ttattcatta ctggaaaacc
      721 attgtgccca tagcgaagca tgattttatt tgcttcttct atagtagtac ttatatcaat
      781 ggttttaaca ggagtagtca ttatgtcaat agccagtgtt tcaacttgaa tatgcttttc
      841 caacaactgc ttaattttat tttcagtatc tattaacgac atgtctctga tcacggcaga
      901 tgctgcctga gaatgacctc ctccccccaa tgtttttaaa atttcaccca cattcactga
      961 attacgatta ctccttgcta ccacatatat tctgttcctc atatttacca gcacaaaaat
     1021 gatatcactg ttcatgattt ctattaattt gtgagtaatt aaagctaaac cttcgatata
     1081 ctcttttgca ttagaaattg caaaaactgc actaatacct tttaaatata attcttgaga
     1141 gttagaaata agctggtt
//
LOCUS       DAOQWT010000548         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_164318_length_1158_cov_1.742520, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000548 DAOQWT010000000
VERSION     DAOQWT010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164318_length_1158_cov_1.742520"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..>1158)
                     /locus_tag="PKJ95_08605"
     CDS             complement(108..>1158)
                     /locus_tag="PKJ95_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66335.1"
                     /translation="STKADWKKSLLWLGISGFFVALTGITRIEGPLVGIAIVICLLLD
                     EIARKSSWKEALPKLLALAVPFLLIYGVYFLLRFLWFKELLPNSYYCKKGFTGNPRTL
                     IDMFLVENKAYLLIGLIGFLNKKARTLLLSMYTYIILLMIGLNGADPIIGHYNRHSLI
                     AVALTGIILSVAIFQLWRIKPIVTAILVFFCIIYNVQTTFHHRERLIAEFSSYEKRME
                     ARAQLADYLNALNISSYAMGDCGLVPYLTPGKKVFDYYGLNSKEFTSWPINRGSEAYI
                     KWLATQSPDAVIVISNDGKSFSSRNKTQKLLYEIFCSQIGYVDINKSFGDGGSIYYAP
                     CYYRILLRKGEKISY"
BASE COUNT          370 a          239 c          203 g          346 t
ORIGIN      
        1 cagatttaag atgattcggg tttgctctac tttaggttcc ctgactagtt ttgggacaag
       61 gtgttctatc attgccagca acgccagtag ataaaaataa acataaatca gtatgatatt
      121 ttctctcctt ttctgagaag tattctataa tagcacggtg cataataaat tgaaccgcca
      181 tcgccaaagc ttttatttat atccacataa ccgatttgac tgcaaaagat ttcatataac
      241 agcttctggg ttttatttct tgatgagaag gacttaccat cattacttat tactatcact
      301 gcatctggtg actgagtggc taaccattta atataggctt cgctacccct gttaataggc
      361 caggaggtaa attctttgga atttaggcca taataatcaa agaccttttt gcctggggta
      421 agatatggta ccagaccgca atctcccatg gcataacttg agatattaag agcatttaga
      481 taatcggcca gttgagctct ggcctccatt ctcttttcat aactcgaaaa ctcagctatc
      541 aatctttctc tgtgatgaaa cgtcgtttgc acgttataaa ttatacaaaa gaaaacgaga
      601 attgcggtga caatgggctt tatccgccat agttggaata tagccaccga tagaattatt
      661 ccagtaagag ccacagctat caatgaatgc ctgttatagt gtcctataat cgggtcagcc
      721 ccatttaaac ctatcatcaa taagataatg taagtataca tagacaagag caatgttctg
      781 gccttcttat ttaagaatcc gataagacca attaaaagat aggctttgtt ttctaccagg
      841 aacatgtcta tcagagtccg aggattcccg gtaaaacctt ttttacaata ataagaattt
      901 ggcaacagtt ctttaaacca aaggaacctt agcaaaaaat aaaccccgta tatcaataaa
      961 aagggaactg ccagggccaa taacttgggc aaagcttctt tccaggaact ttttctggca
     1021 atttcatcta ataaaaggca aataactatc gcgatgccca ccaagggacc ttctattctg
     1081 gtaattcccg tcagggcaac aaaaaaacct gaaatcccaa gccataaaag tgatttcttc
     1141 caatctgctt tcgtactt
//
LOCUS       DAOQWT010000549         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_164426_length_1157_cov_2.532668, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000549 DAOQWT010000000
VERSION     DAOQWT010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164426_length_1157_cov_2.532668"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1157)
                     /locus_tag="PKJ95_08610"
     CDS             complement(<1..>1157)
                     /locus_tag="PKJ95_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNR66336.1"
                     /translation="SERRDFSEEERIVNLFHTLFDIEPYLSEDYVNEDYDYDRFMKQF
                     FGIYQELQQTEQEIEGKLGEIARKKEYIDNLKYLSSFNLEINRFIDMEYLVFRVIRIT
                     RENYDKLKKNYKNIPAIILKVAAEGKNVILVSIAPETLEETLERIYSSLNYTILPLPQ
                     EMEGTVDVVSEKLRSSIEEDQKIIESLKISIEKYKEQYINELKKMHSRLEMEKKIEEV
                     KSKIALGDKSFFMFGFVPASRVNKLKGELEKYFDDRLIILIDEIKKPYSGTTTPPTKL
                     NNIKLFQPFESLVKMYGIPSYQEKDPTVFFALTYLLIFGAMFGDVGQGAVLLLAGIIL
                     EYFYKKVDFGGILNRLGLSSIIFGFLYGSVFGSEDILPALVIRPMSNINNM"
BASE COUNT          345 a          236 c          170 g          406 t
ORIGIN      
        1 catattgttg atattggaca tcggtctgat aaccagagcc ggcagaatat cttcagaccc
       61 gaaaacactc ccgtataaga aaccaaagat gatagaactc aatcctaatc tattcaaaat
      121 cccgccaaaa tctacttttt tgtaaaaata ttctaagatt ataccagcta aaagcagaac
      181 tgctccctgt cccacatcac caaacattgc cccgaagatg agcagatagg tcaaggcaaa
      241 aaagacggtg gggtcttttt cctgatacga agggatacca tacatcttta ccagactttc
      301 aaaaggctgg aataatttta tattattaag tttggtggga ggggtagttg tacctgaata
      361 aggtttttta atctcatcaa ttaaaatgat taatctatca tcaaaatatt tttctaattc
      421 tccttttaat ttattaaccc tgctggctgg tacaaaacca aacataaaga atgatttgtc
      481 accaagagca atttttgatt taacctcttc aatttttttc tccatttcca accgggaatg
      541 catctttttt agctcattta tgtactgttc tttatatttt tctatcgata ttttcaaaga
      601 ctcaattatc ttttggtctt cttcgatgct gcttctcagc ttttctgaaa ccacatctac
      661 tgttccttcc atttcttgag gcagtgggag aatggtataa ttcaacgaac tataaatcct
      721 ctccagggtc tcctccaacg tctccggtgc gattgaaacc agaataacat tcttaccttc
      781 tgctgcgact tttaaaataa tggctggaat attcttataa ttctttttta gcttatcata
      841 gttttctctg gtaattctaa tcactctgaa taccaaatat tccatatcta tgaagcggtt
      901 aatttctaag ttgaatgaag acaggtattt taagttgtca atatattctt tttttctggc
      961 aatttctcct aatttgcctt caatctcctg ctcggtctgc tgtagctcct ggtatatccc
     1021 aaaaaattgt ttcataaacc ggtcatagtc gtaatcttca tttacataat cttcactcaa
     1081 atagggctct atatcaaaga gggtatgaaa aagatttaca attctttctt cttcagaaaa
     1141 atctcttctc tctgaat
//
LOCUS       DAOQWT010000550         1154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_165106_length_1154_cov_3.029117, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000550 DAOQWT010000000
VERSION     DAOQWT010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165106_length_1154_cov_3.029117"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /gene="hypD"
                     /locus_tag="PKJ95_08615"
     CDS             complement(<1..927)
                     /gene="hypD"
                     /locus_tag="PKJ95_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNR66337.1"
                     /translation="MKYYDEYRDKNLCQLIIEKIKKLTKEEIRIMEVCGTHTMSIFRY
                     GIKNLLPANISLISGPGCPVCVTPINEIDHMISLSDMEETIITTFGDMINVPGSTSSL
                     KKKKAEGADIRVIYSPLQALGIAEQFPQKRVILIGIGFETTIPLLASTVLEAKKRRID
                     NFFLFSLAKLMPPIIKTLLESNEVKIDGMLCPGHVSTIIGTKPYQFIPERYDIACVIA
                     GFEPVDILNAIYHIIEQRIKKIPQVENKYRRAVREEGNPLALALINQVFLTVPSEWRG
                     IGMVAESGLELRPEFSNLNARNFSVKIEREREN"
     gene            complement(924..>1154)
                     /locus_tag="PKJ95_08620"
     CDS             complement(924..>1154)
                     /locus_tag="PKJ95_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNR66338.1"
                     /translation="RRSICMCLAIPGKVLKKVNSIEAEVLIGKIKKLVRIDLFPEIKE
                     GDYVLIHAGYAITKIDPEEANEINQLWEEMHS"
BASE COUNT          318 a          260 c          187 g          389 t
ORIGIN      
        1 attctctctt tctctttcta tttttaccga aaaattacgg gcattcaaat tagagaattc
       61 cggtctcagc tctaagccgc tttctgccac cataccgatg cccctccact cactaggaac
      121 cgttaaaaat acctggttta ttaaagcaag ggctaaagga tttccttctt ccctcaccgc
      181 tcttctgtat ttattttcta cctgcggaat ctttttaatc ctctgttcaa taatatggta
      241 tatggcattt aagatatcaa ccggttcaaa tccggctatt acacaggcaa tatcataacg
      301 ttcaggaatg aattgatatg gtttggtgcc aataatagta ctgacatgcc cggggcagag
      361 cattccgtct atcttcacct cattactctc caatagcgtt ttgataatcg gaggcattaa
      421 tttagccagg ctgaacagaa aaaaattgtc aatccgtctc ttctttgctt ccaaaacagt
      481 actggctagc aatggtatgg ttgtttcaaa accaattcct atcagaatga ctcgtttctg
      541 aggaaactgc tctgctatcc ccaacgcctg taggggagaa tatattaccc tgatatctgc
      601 cccttcagct tttttctttt ttaaactgga agtgctgccg gggacattaa tcatatctcc
      661 aaaagtagtt attatggttt cctccatatc agaaagtgat atcatatggt ctatttcatt
      721 aatgggagta acacataccg gacacccggg tcctgaaatc aaactaatat tcgccggtaa
      781 aagattttta atgccgtaac gaaaaatgga catggtatgg gtaccacaga cttccattat
      841 tcttatttct tcttttgtta attttttaat cttttctatt atcagctggc aaagattttt
      901 atccctgtat tcatcataat atttcaagag tgcatctcct cccagagctg gtttatctcg
      961 tttgcctctt caggatcgat tttggtaatg gcatatccgg catgtataag cacataatct
     1021 ccttctttaa tttcaggaaa aaggtcaatc cttaccaact ttttgatctt tcctataaga
     1081 acctctgcct ctatagaatt gacttttttt aaaaccttac caggaatcgc aagacacata
     1141 cagatactcc ttct
//
LOCUS       DAOQWT010000551         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_167273_length_1146_cov_1.874427, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000551 DAOQWT010000000
VERSION     DAOQWT010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167273_length_1146_cov_1.874427"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PKJ95_08625"
     CDS             complement(<1..679)
                     /locus_tag="PKJ95_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNR66339.1"
                     /translation="MSEKQRFVHLHVHSEFSLLDGACRIKELIRKAKENNMSAIALTD
                     HGVMYGAIQFYKEAVANNIKPIIGCEVYLAPNSRKDKSYGKKESPSHLILLAKNKRGY
                     QNLIQLVTRSFLEGFYYKPRVDKEILREYNEGLVALSACLKGEIPRLILQNNFSEAEE
                     LALSYQEIYGKGNFYLEIQNNTMPEQIKVNQELIKLSRSLEIPLVATNDVHYLNKEDF
                     EAHDILLC"
     gene            complement(924..>1146)
                     /locus_tag="PKJ95_08630"
     CDS             complement(924..>1146)
                     /locus_tag="PKJ95_08630"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNR66340.1"
                     /translation="VGHMQRGGNPTVFDRILGLRTGVKAVELIEKENFGKMAALKGNK
                     IVPVSLEEAISKIKTVNKEWIDFAKIFFK"
BASE COUNT          339 a          229 c          178 g          400 t
ORIGIN      
        1 tacataataa gatatcatgt gcctcaaaat cttctttgtt gaggtagtga acatcattgg
       61 tagcaaccag cggaatttca agggaacggc tcaatttgat cagctcctgg ttaaccttaa
      121 tctgttccgg catagtgttg ttttgtattt ccaggtaaaa atttcccttg ccatatattt
      181 cctgatagga gagagccaac tcctctgctt cactaaagtt attttgaagg attaaacggg
      241 gtatttcccc ttttaaacat gccgacaaag caacaagtcc ttcgttgtat tctctcaata
      301 tctctttatc tactctcggt ttataataaa aaccttctaa aaatgatctg gtgactaact
      361 ggataagatt ctgataccct ctcttgtttt ttgctaaaag tatcagatga ctgggagatt
      421 ccttcttgcc ataagattta tccttcctgc tatttggagc aagataaacc tcgcacccta
      481 taatgggttt gatattattg gcaaccgctt ctttatagaa ctggatagca ccatacataa
      541 ctccatggtc agttaaggca atagcagaca tattgttttc tttggctttt cttattaact
      601 ctttgatacg gcatgctcca tccaagaggc taaattcaga gtgaacatgc agatgaacaa
      661 atctttgttt ttcagacata gtatcttccc tcttcagcaa aaatcatttc atttcagcaa
      721 cttgacctta ccttgtgtca catttaaaat ctaaaagaaa ttttcggaaa tagccatttt
      781 ttagtattct tagattatta tatactaatt gaggtggttt tcatattaaa agagaatctg
      841 ttattataaa aaaggggtat cgggttaatt ttttccaaaa tattataata atagaacaac
      901 ttaaacaggc agattttttg taatcattta aagaaaattt tcgcaaaatc aatccactct
      961 ttatttacgg ttttaatttt actgattgct tcctccaaag acacgggaac aattttattt
     1021 cccttaagag ctgccatttt cccaaaattt tccttttcaa ttaattccac agcctttact
     1081 ccggtacgca agcccaggat tcggtcgaat accgttggat tacctcccct ttgcatatgt
     1141 ccaacc
//
LOCUS       DAOQWT010000552         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_168120_length_1142_cov_2.857406, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000552 DAOQWT010000000
VERSION     DAOQWT010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168120_length_1142_cov_2.857406"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PKJ95_08635"
     CDS             <1..528
                     /locus_tag="PKJ95_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNR66341.1"
                     /translation="AKSFIKSVQQQFPDATHHCWAYRLGSGKIEIAQYSDGGEPPNSA
                     GPPILQAIKQEQVSNVIALVVRYFGGIKLGKSGLIKAYRDTARMGLQLAGKKNKLPMQ
                     ELVMEGIEYNSLGTILQSIESKSGKIEDIQYGEKVNVSALLPAVEQEWFKKLFNNLTR
                     GKASLKVGEIKWITV"
     gene            571..1065
                     /locus_tag="PKJ95_08640"
     CDS             571..1065
                     /locus_tag="PKJ95_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNR66342.1"
                     /translation="MSRIIKILVVYYSRSGKTRNIASIIAKELEAETEEIVDHKNRKG
                     LLGFISSGNEAYLQRVIPIEKLKKDPAGYDLIIIGTPVWAGYLSSPVHSFLREYKEKI
                     AKFAFFITCMGSDPGRILLNVEHIMSKKPVAVMNINHRALKNQYHLKMIDEFVSSLKQ
                     YIKD"
BASE COUNT          419 a          163 c          229 g          331 t
ORIGIN      
        1 gcaaaatctt ttatcaaatc tgttcagcag caatttccag atgctactca tcattgttgg
       61 gcttatcgcc tgggttctgg aaaaattgaa attgcacaat attctgatgg cggtgaacct
      121 cctaattctg ccggccctcc aattttacag gcaattaaac aggaacaggt aagtaatgtt
      181 atagccctgg tcgttcgcta ttttgggggg attaaattgg gcaaaagcgg attaattaaa
      241 gcatatagag atactgctcg aatgggatta caattagcag gtaagaaaaa caaattacca
      301 atgcaggaat tagttatgga aggtattgaa tataactcgt tgggaacaat cttacaatct
      361 attgaaagca aatcgggaaa gattgaagat attcaatatg gagagaaagt aaatgtttca
      421 gccttactcc ctgcagttga acaggaatgg tttaaaaaat tgtttaataa tcttacccgg
      481 ggtaaagcct ctcttaaagt aggagaaatt aaatggataa ctgtttgatt gggttttaaa
      541 atatttatat caccataaat aaaaggaaag atgagcagaa ttataaaaat attagtagta
      601 tattacagtc gttcaggaaa aaccagaaat attgccagta ttattgctaa agaattagag
      661 gcagaaacag aagaaattgt tgaccataaa aacaggaaag gtcttttagg atttatatcc
      721 agtggaaatg aagcctatct gcaaagggtt atacctatcg aaaaactaaa aaaagaccct
      781 gccggatatg atttaattat tatagggaca cctgtctggg caggatacct atcatcacca
      841 gttcacagtt ttttaagaga atataaggaa aagatagcca aatttgcttt ttttatcacc
      901 tgtatggggt ctgatccggg gaggatttta ctgaatgtag aacatataat gtccaaaaag
      961 ccggtggcag tgatgaatat aaaccatcga gctcttaaaa accaatacca tttaaaaatg
     1021 attgatgagt ttgtttccag tttaaaacaa tatataaagg attaataatt atataatgaa
     1081 tgaaaaaaag tttgaacaat tggtaattga agcgataagt gaattaccgg aatactttcg
     1141 tg
//
LOCUS       DAOQWT010000553         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_168699_length_1140_cov_2.647005, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000553 DAOQWT010000000
VERSION     DAOQWT010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168699_length_1140_cov_2.647005"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PKJ95_08645"
     CDS             complement(<1..699)
                     /locus_tag="PKJ95_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNR66343.1"
                     /translation="MEIIMVPGAAGFIGSKVSEYLVNKGYQVIGLDNLNHYYDVRLKE
                     KRLDNLVRLGNFRFFEVDIENYDSLETLFQKYDFKGIINEAARAGIRASIDNPLAYQK
                     TNIEGNLNLLELCKKYHVQHYVLASTSSVYSGCKQPFQEEFITDTLYSPYAATKKSAE
                     LMAYTYHYLYQINVVVLRYFTVYGPFGRPDMVVFRFIKWIMEGKKVQIYGDGQQKRDF
                     TYIDDIAEGTIRALG"
     gene            complement(809..>1140)
                     /locus_tag="PKJ95_08650"
     CDS             complement(809..>1140)
                     /locus_tag="PKJ95_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66344.1"
                     /translation="AKVLIGDVGTLSIGATLASVAIIGNFQTAGVILIIPYFIDFLFK
                     AFNRFPSENWWGIYKGGKLYCPESGPVGFAQWIMRIFNGITERNLVIVFMIIEGFFAI
                     LAIKIYL"
BASE COUNT          379 a          238 c          137 g          386 t
ORIGIN      
        1 tcccaatgcc cttatggttc cctcggcaat atcatcaata taggtaaaat ccctcttctg
       61 ctgaccatca ccatatatct gaactttttt cccttccata atccatttaa taaatctaaa
      121 aacaaccata tcgggacgac cgaatggtcc atacactgta aagtaacgca gaacaacaac
      181 attaatctgg tacagatagt gataagtgta agccattaac tctgcagact tcttggtggc
      241 agcataaggc gaataaagag tatcagttat aaattcttct tgaaaaggtt gtttacagcc
      301 agaatatact gatgaggtag aggcaagtac ataatgttgt acatgatatt ttttacaaag
      361 ctccaataaa ttcaaatttc cctctatatt ggttttctga tatgctaatg gattgtctat
      421 actcgccctg ataccagccc gggcagcttc attaattatt cctttaaagt catatttttg
      481 aaacaatgtt tctaatgaat cataattttc gatatcgacc tcaaaaaatc taaaattacc
      541 taatcttact aaattatcca atctcttttc ttttaacctg acatcataat aatgattgag
      601 attatccaat cctattacct gatatccttt atttacaaga tattctgaaa ccttgcttcc
      661 tataaatcct gctgcccccg gtaccattat tatttccatt tttatacctt tcgatttatt
      721 cctcaactat attataaata attttgcgaa gatttcctgc cttaatattt ccttaaatta
      781 aactatatta actattattc taattatttt ataagtaaat ttttatagct aatattgcga
      841 aaaatccttc tattatcata aaaacaataa ctaaatttct ttccgtaatt ccattgaata
      901 tcctcataat ccattgggca aaccctaccg gaccactttc aggacaatac agtttacccc
      961 ctttatatat gccccaccaa ttttcactgg gaaatctatt aaacgcttta aataaaaaat
     1021 caataaaata agggataatt aaaataacac cggctgtttg aaagtttcca attattgcta
     1081 cactggccag ggttgccccg atagaaagag tccccacatc accaattaat actttagcag
//
LOCUS       DAOQWT010000554         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_169407_length_1137_cov_3.256932, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000554 DAOQWT010000000
VERSION     DAOQWT010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169407_length_1137_cov_3.256932"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1137)
                     /locus_tag="PKJ95_08655"
     CDS             complement(<1..>1137)
                     /locus_tag="PKJ95_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNR66345.1"
                     /translation="YKSSKLLLRHFPTHAEWVLFGPGENAGAIDIGDGMAMVMKIESH
                     NHPSAVEPFHGAATGIGGVVRDILAMGARPVAILGSLRFGNWEDPHIQYLVKEVVKGL
                     AFYANNILVPVIGGETYFLDSYQENPLVNAMCIGLVKKEDIVIAAAKEVDSIVILAGE
                     RTGRDGVGSASFASRELSESDEEINHPPIPQGNPYLKKKIIEACLEIVANGLILAMQD
                     LGAAGLVSSSSEMASKGNSGIELNLTLVPKKEKDITSREIMLSETQERMLLIARKEKL
                     KEIGLVLDKWGIDYTVIGRVTSDGLLRLKERGNIVAEVPAKSLAEGAPYRHYQGRISD
                     KFKEIQKLDLGKIPQPLDYNQALLGLLSSRNLCSKRGIYNSFNQ"
BASE COUNT          302 a          289 c          182 g          364 t
ORIGIN      
        1 tttgattgaa actattataa atccctcttt tgctacaaag atttctggaa gatagcaatc
       61 ccagcaaagc ctggttgtaa tccaggggtt gtggtatttt tcccagatcc aatttttgta
      121 tttctttaaa cttatcagaa attcttcctt gataatgacg atagggagct ccctccgcta
      181 aagactttgc cggtacttcg gcaactatat tacccctttc ttttagtcgt aacaacccat
      241 ctgaagtcac cctacctata acagtatagt ctatgcccca tttatctaat accaatccta
      301 tttcttttag tttttctttt ctagctatta ataacatcct ctcctgagtc tctgaaagca
      361 ttatttcccg ggatgtaatg tctttttctt tttttggcac taaagtcagg tttaattcga
      421 ttcctgaatt acccttgcta gccatttcag agctagagct caccagaccc gctgccccaa
      481 ggtcctgcat tgccagtatc agaccatttg caactatttc caggcaggct tctataatct
      541 tcttctttaa atacggatta ccctgtggaa tgggtggatg attgatctct tcatctgatt
      601 ctgataattc ccgcgaagca aagctggcac ttcctacccc atcccttcca gtcctttctc
      661 ctgccagaat aacaatacta tcgacctcct ttgctgctgc aataacaata tcttcctttt
      721 ttaccagacc tatgcacatg gcatttacca gaggattttc ctgataacta tcaagaaaat
      781 aggtctctcc gcctattacc ggtactaaaa tattgttggc ataaaaagca agccctttca
      841 ctacttcttt taccagatat tgtatatgag ggtcttccca atttccaaat cgaagagaac
      901 ccaaaatggc taccggcctg gcacccatag ccagaatatc ccttacaact cctcctatac
      961 cggtagcagc tccatgaaaa ggctcaaccg ctgaaggatg gttgtggctt tcaattttca
     1021 tgaccattgc cattccatcc cctatgtcaa tagccccggc attttcacca ggtccaaata
     1081 aaacccattc agcatgagtg gggaaatgtc ttaagagaag cttagaactt ttgtaac
//
LOCUS       DAOQWT010000555         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_169952_length_1135_cov_2.618519, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000555 DAOQWT010000000
VERSION     DAOQWT010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169952_length_1135_cov_2.618519"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PKJ95_08660"
     CDS             complement(<1..684)
                     /locus_tag="PKJ95_08660"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNR66346.1"
                     /translation="MSIIVFNLLGGLALFLYGLYVLSDGLKQVFYKGLKDILKKVTSS
                     TLKTVGFGAFITAVIQSSSITVVTLVGLLNSSMLNLKQAIGVMLGAKIGTTITTQIIA
                     FKIGFYFFPFIIIGSFLFFLFREKRIQNIGQIILGFGILFLGMNFMSEGAKVIQEIPF
                     FENALYYFSNNITMGIITGAILTAIVQASSVTIALSIALAMQGALTLPAAIAITMGAN
                     IGTAITGFFA"
BASE COUNT          433 a          178 c          162 g          362 t
ORIGIN      
        1 agcaaaaaaa ccggtaatag cagtaccaat attggctccc atagtgatgg ctatcgctgc
       61 tggtagagtt aaggcaccct gcatagccaa tgcaatactg agggcaatag ttactgaact
      121 ggcctgaact attgccgtta atatagcccc ggtaataatc cccatggtta tattgttgct
      181 aaaataatat agggcattct caaaaaaagg aatctcctgt ataactttgg caccttcaga
      241 cataaagttc attcctaaaa aaagtatgcc aaatcccaga ataatttggc caatattttg
      301 tattcttttt tctctgaaaa ggaaaaatag gaaggaacct ataatgatga aaggaaaaaa
      361 gtaaaatcca attttaaaag caattatctg ggtagtaatt gttgtgccaa tttttgctcc
      421 caacattaca ccaattgctt gctttagatt tagcatgctg gagtttagaa gtcctaccag
      481 agtgactacg gtgatacttg aactttgaat taccgcggtg ataaatgcac caaagccaac
      541 cgtttttaga gtactgctgg ttactttctt taaaatatct ttaaggcctt tgtaaaaaac
      601 ttgttttaat ccatcgctta aaacatacag gccatacaaa aaaagagcta aaccacctaa
      661 aagattaaaa actatgatac tcatactatg atactcatac tatgatatat ctccttaatt
      721 ttgctagtaa ttttgaaaca aataaaaata aaatatttta aaataaatat tttattaata
      781 gactgactac caatactttg tcgaagaaag aaaaagaaca ggattattta aatagttagg
      841 aaaattctaa aattctcata catatccata cctatacaag tattatatta tcactttctt
      901 caaaatctga caaaaaatat taaaaaatct ttattaaaat tttaaaaacc tgaaataaat
      961 ataatattta agaatatcca gtatttttca cttattttgt taaattatcg gactgatttt
     1021 ataataccta ttgaactgta tcattaaatc tgataatcat actgtagaga aagagtgaat
     1081 aaatggaagt aaaaatatgt tatttaattt ataacaggat tagtagaagc tactt
//
LOCUS       DAOQWT010000556         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_170910_length_1131_cov_3.441450, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000556 DAOQWT010000000
VERSION     DAOQWT010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170910_length_1131_cov_3.441450"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKJ95_08665"
     CDS             <1..309
                     /locus_tag="PKJ95_08665"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNR66347.1"
                     /translation="PLDLTSIDLNNESIIAPKVISFQPPEYPDNFRRRGIEGRVQIKA
                     LIDKKGDVVEIMIDTSSGYPGFDRNAIESISKWKFQPAYYNNEERACWVLIPIIFQLK
                     "
     gene            330..956
                     /locus_tag="PKJ95_08670"
     CDS             330..956
                     /locus_tag="PKJ95_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNR66348.1"
                     /translation="MKILIEQGGIVIYPLLTLSVVTLAIIIERFFYFIRIRKNIPEKV
                     MSKIKNNLQRANSMDEISFLKNSPNPICHVLYNGIVAWRENSLEIERAMEETKLIEFP
                     KMEKNLTMLHFIAKISPSLGLLGTVTGMIKTFHFLSLDIEPQLLAQGISEALITTAFG
                     LSLSIPAFAAYYYFMNKIENLIKNAEKRELELINHIQKLGNKDAQIQA"
     gene            937..>1131
                     /locus_tag="PKJ95_08675"
     CDS             937..>1131
                     /locus_tag="PKJ95_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNR66349.1"
                     /translation="MRKYRLKKIDDMEINIASLVDIIFLLLLFFVVASTLDTGIIRAT
                     VRLPNSGELSNIEKIDSITLF"
BASE COUNT          397 a          181 c          193 g          360 t
ORIGIN      
        1 ccccttgact taacaagtat cgatttaaat aatgaatcta tcattgctcc aaaagtaatc
       61 tctttccagc caccagaata ccctgacaat tttagacgta gaggtattga aggaagagtt
      121 caaattaagg cattgataga caaaaaaggt gatgttgttg aaattatgat agatacctcc
      181 tcaggttatc ccggttttga ccgaaatgct attgaatcaa tttcaaaatg gaaatttcaa
      241 ccagcatatt ataacaatga ggaaagagcc tgctgggttt taatcccgat aatttttcaa
      301 ttaaaataga tttagaaaga ggatgaaaga tgaaaatatt aattgaacag ggaggaatcg
      361 tgatctatcc ccttttaacc ctatctgttg taactcttgc aataatcata gagcgtttct
      421 tttattttat tagaatcaga aaaaatattc ctgagaaggt gatgtccaaa attaagaata
      481 atttacaacg tgctaattcg atggatgaaa tcagtttttt aaaaaattct cccaacccca
      541 tatgtcatgt tttatacaac ggaatcgtag cctggagaga gaatagttta gagattgaaa
      601 gagctatgga agaaacaaaa ttgatagaat ttcctaaaat ggagaaaaac cttactatgt
      661 tgcattttat agcaaaaata agtccttccc tgggattact gggaacagtt accggaatga
      721 taaagacttt ccatttcctg tctttagata ttgaaccaca attattggca cagggaatat
      781 ctgaagcact aatcactact gcctttggac tgagcctttc tatccccgcc tttgccgctt
      841 attattattt tatgaataaa atagaaaatt tgataaaaaa tgctgaaaaa agagagctgg
      901 aattaattaa ccatatacaa aaactgggta ataaagatgc gcaaatacag gcttaaaaaa
      961 attgatgata tggaaataaa tattgcttct cttgtcgata taatttttct tctacttctt
     1021 ttttttgttg ttgcttcaac tcttgataca ggcattatta gggcaactgt aaggcttcct
     1081 aactctggag aactgtctaa tattgagaag atagactcaa ttactctttt t
//
LOCUS       DAOQWT010000557         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_171661_length_1128_cov_2.719478, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000557 DAOQWT010000000
VERSION     DAOQWT010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171661_length_1128_cov_2.719478"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PKJ95_08680"
     CDS             <1..971
                     /locus_tag="PKJ95_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66350.1"
                     /translation="LSSNLVLLYSSQDSTGKQIDHWLRPQWQFSYTGQGYSLNLITEK
                     LIDLNLWGNPDGISLNKPGTLDRLPELVFNKSSTKLFDTSINYSINASLGRFYESATD
                     QENIRGEYIINVNRPFKITDNISLNASGIYRQDVYLTGEARYMLGGKLDLKVGFQPGF
                     SGNFSYNYYMSEGPTPFNFDALSPLTESASASIVLKPRDNLQINLSTNYNFVSESFGS
                     LGARVQWKPKDDYNINLNTYYDLNKKSWNKRVDAKMSLKLSDDWRLTYSGSLYFDKLD
                     IKNSVISVVRDLHCREISINYRQSTKSVWVDFRINAFPTESFTIGS"
BASE COUNT          380 a          196 c          211 g          341 t
ORIGIN      
        1 acctaagctc aaacctggtt ttactatatt cgtcacagga ttccacagga aagcagattg
       61 accactggtt gcgcccacaa tggcaatttt cctatacagg acagggttat agtcttaacc
      121 tgataaccga aaaattaatt gatttaaatt tatggggaaa tcctgatgga attagcttga
      181 ataaaccagg caccctggat agattaccgg aattggtatt caataaaagc agtaccaaac
      241 tatttgatac cagcataaat tacagcatca atgcttcact gggcagattt tatgagagtg
      301 caacagacca ggaaaatatt agaggagaat acattatcaa tgtcaacaga ccttttaaga
      361 ttactgataa tattagttta aatgcatcag gcatctatcg acaggatgta tatttaactg
      421 gtgaggcacg ttatatgctg ggtggaaaat tggatttaaa ggtaggattt caacctggat
      481 tttctggtaa tttttcctat aattactata tgtctgaagg acctactccc tttaattttg
      541 atgcattaag ccctctaacc gaatctgcca gtgccagtat tgttttaaaa ccaagggata
      601 atttgcagat taatctttct acaaattaca actttgttag cgaatctttt ggtagcttag
      661 gtgccagagt acaatggaag ccaaaagatg attataatat taatctaaat acctattatg
      721 atttgaataa aaagagctgg aataaacgag tagatgctaa aatgtcttta aaattaagtg
      781 atgactggag attaacttac agcggttctc tttactttga taaacttgat ataaagaata
      841 gtgtaataag tgtagtcaga gatctgcatt gcagagagat tagtatcaat taccgtcaat
      901 ccactaaatc ggtctgggtt gactttagaa tcaatgcctt ccccaccgaa tccttcacca
      961 tcggcagcta accctgtgac acaagggaaa gggaaacttt ggagatatcg aaatcaggac
     1021 caccttcaga atcaccggta aagtcctgaa caattttact aaagtttgtt atactggcat
     1081 caatagtcaa atctaaaggg ttattcatag aatcatagct tttatctt
//
LOCUS       DAOQWT010000558         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_174054_length_1119_cov_2.575188, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000558 DAOQWT010000000
VERSION     DAOQWT010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174054_length_1119_cov_2.575188"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="PKJ95_08685"
     CDS             complement(<1..1111)
                     /locus_tag="PKJ95_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNR66351.1"
                     /translation="MKVTGPQEIPTDITDEIKTSYLNYAMSVIVGRAIPEVRDGLKPV
                     HRRVLYSMYKLGNTPDKPYKKSARVVGDTLGKYHPHGDMAVYDTIVRMAQDFSYRYPL
                     VDGQGNFGSIDGDAAAAMRYTEIRMSALAQEMLADIKKDTVDFIPNFDNSLEEPTLLP
                     APVPQLLINGASGIAVGMATNIPPHNLGEVIDACVLLIDNPEATLEDIMKVLPGPDFP
                     TGATIYGVSGIKEGYQTGKGIITLQAKVISENLEKKSQNAPVLVVKELPYQVNKANLV
                     EQIALLVQDKKINDITALRDESDRNGMRIVIELKKNANANVLLNNLFKHTNLQTTFGI
                     NMLAIVDGKPKQLSLRESLDYFLAHRKTVVERRSRY"
BASE COUNT          307 a          246 c          218 g          348 t
ORIGIN      
        1 cataacggga gcgccgctcc actactgttt tacgatgggc taaaaaataa tctaaagatt
       61 cgcgtaggct gagctgtttt ggctttccgt ccacaatggc cagcatgtta atgccaaaag
      121 tagtctgcag attggtatgt ttaaacagat tgttcaacaa aacattggca ttggcatttt
      181 ttttcaattc aataacaatg cgcattccat tcctatctga ttcgtctctt aaggcagtga
      241 tatcattgat tttcttatcc tgcactaata atgctatctg ttccactaaa ttggctttat
      301 taacctgata ggggagttct tttactacca ggaccggggc attttgactt tttttctcaa
      361 gattttctga aattacttta gcctgcaagg taataatacc ttttccggtc tgatagcctt
      421 cttttattcc agaaacaccg tatatagtgg cacctgtagg aaaatcaggt cccggcagca
      481 ctttcataat gtcttctaag gtcgcttctg gattatcgat aagtaaaaca caggcatcaa
      541 ttacttcccc caaattatga ggagggatat tggtagccat acctactgca ataccggacg
      601 caccattgat taacaattga ggaacgggag ccggcaatag ggtaggctcc tccagtgaat
      661 tgtcaaaatt aggaataaaa tcaacggtgt ctttttttat atcggcaagc atctcctggg
      721 caagagctga cattcttatt tcggtgtaac gcatagcagc agctgcatca ccatcaatag
      781 agccgaaatt gccctgccca tccaccagag gataacggta ggaaaaatcc tgtgccattc
      841 tgacaatggt gtcatatacc gccatatctc catgagggtg gtatttaccc agagtgtcac
      901 ccacaactct ggctgatttt ttatatggtt tatcaggggt attccccaat ttatacatag
      961 aatagagtac acgtctgtga acaggcttta atccatctct tacttccggg atagccctgc
     1021 cgacaatcac actcatggca taattcaaat aagatgtttt aatctcatcg gtaatgtcag
     1081 taggtatttc ctgaggccct gttactttta tcttttctt
//
LOCUS       DAOQWT010000559         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_175047_length_1115_cov_3.161321, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000559 DAOQWT010000000
VERSION     DAOQWT010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175047_length_1115_cov_3.161321"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1115)
                     /locus_tag="PKJ95_08690"
     CDS             complement(<1..>1115)
                     /locus_tag="PKJ95_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNR66352.1"
                     /translation="QGLTDVAQNKDYRGEEVISAYTYIPELNWGFITKQDIRETYEPL
                     KNLLINLVLLFLVIMIVIFLVARGLAQSITRPLRDMSTVSLKIQRGDYSQRVTETGLD
                     EVSNLAKTINKTSEVIQRAISIRKFRTGILENIIKTTDIYQFYGQIIYQLCNYSESQV
                     GVFYCLNKEKQQFEHQASVGIDRELIKTFDLNYREGIIGQVLNKQGIVYLKDIPADTI
                     FAYKTIPGNMVPREIIAFPLVLNDQIDAVIILASIYPYHSDILEMISQSAPAIAITME
                     KLFNEEESRRLNKEIEDRNILLNQLNTELQEQAEELKNQTEELERQNIELEQQKNEVQ
                     EANRLKSEFLSSVSHELRTPLNSIIALSDLLEIQLGT"
BASE COUNT          312 a          222 c          198 g          383 t
ORIGIN      
        1 tgttcccagt tgtatttcta atagatcaga aagtgcaatg atagaattca ggggggtgcg
       61 taattcatgg ctaacactgg atagaaattc actcttcagg cgattagcct cctgcacttc
      121 attcttctgt tgttccagtt caatattctg gcgttccaat tcttcagtct gatttttcag
      181 ttcttctgcc tgttcttgta attcagtatt taattgattt aacaatatgt ttctatcctc
      241 aatttccttg tttaaccgcc tgctctcctc ttcattgaac aatttttcca tagtaatggc
      301 tatggcagga gcagactggg aaatcatctc taaaatatca gaatggtagg gatagatgct
      361 ggctaaaata attacagcat ctatctggtc gttcaagaca agggggaaag caattatctc
      421 tctgggaacc atattgccag ggattgtttt ataggcaaaa atagtatcag cgggaatatc
      481 tttcagatag acaattcctt gtttattcag aacctggccg ataattcctt ctctatagtt
      541 caaatcaaag gtttttatca attccctgtc tattcccact gatgcctggt gctcaaattg
      601 ttgtttttct ttatttaagc aatagaatac ccctacctgt gactcagaat aattgcataa
      661 ctggtagata atctgtccgt agaactgata aatatcagtt gttttaataa tattctccag
      721 aatgccggtt cgaaacttac ggatgctaat agcacgttgt atgacctcag aagtcttatt
      781 tattgttttt gctaaattgc ttacctcgtc taaaccagtt tctgtaactc tttgagagta
      841 atcccctctc tgaatcttta aagagaccgt gctcatatcc cttaagggtc tggtaattga
      901 ttgggcaagt cctctggcaa caaggaaaat gacaatcatt ataaccagaa ataataaaac
      961 taaattaatc agtaaatttt ttaacggctc ataggtttct cgaatatctt gcttggtaat
     1021 aaagccccag tttagttccg gtatataagt ataggcagat atgacttctt cgccccgata
     1081 gtctttattc tgcgccacat cggtcaaccc ttgta
//
LOCUS       DAOQWT010000560         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_177489_length_1106_cov_4.743102, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000560 DAOQWT010000000
VERSION     DAOQWT010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177489_length_1106_cov_4.743102"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PKJ95_08695"
     CDS             complement(<1..1092)
                     /locus_tag="PKJ95_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR66353.1"
                     /translation="MPEINLSPSFGTIEPNSSVICILNFIPGDSLGTYNYELQLSSND
                     PVSHLITVPVEYVVTSPIAYIPDDNFRMVINEALGQPSDYQPTIADLNGLTGTLSAWW
                     RNIVSIEGAQYLINLQRLSLSSNLISDLSPLAGLTNLNLIFLYDNQISDLSPLAGLTH
                     LQSLDLSYNQISDLSPLAGLTNLQGMYLHNNQISDLSPLAELANLWYIYLYDNQISDI
                     SPLAGLINLQYLLLNNNQISDLSPLAGLTNMQGMNLSSNQISDLSPLAELTYLQDLWL
                     HNNQISDLSPLAGLTILLQLGLCNNQISDLSPLAGLSNLQGLSLDNNQISDLSPLAEL
                     TYLYELILEYNQISDLSPLAGLTNLQELNL"
BASE COUNT          364 a          208 c          228 g          306 t
ORIGIN      
        1 aagattaagc tcttgcagat tagtcagacc cgctaaagga ctgaggtcac ttatctggtt
       61 atattcaaga ataagctcat acagataagt cagctctgct aaaggactga ggtcacttat
      121 ctggttatta tcaagcgaca acccttgcag attagatagc cccgctaaag gactgaggtc
      181 acttatctgg ttattacaaa gaccaagttg tagcagaatg gtcagccccg ctaaaggact
      241 gagatcactt atctggttat tatgaagcca caaatcttgc agataagtca gctccgctaa
      301 aggactgagg tcacttatct ggttactact aagattcata ccttgcatat tagtcagccc
      361 cgctaaaggg ctaagatcac taatctggtt attattaagt aaaagatatt gcagattaat
      421 cagccccgct aaaggactga tgtcacttat ctggttatca taaagataaa tataccacaa
      481 attagccagt tccgctaaag gactgaggtc acttatctgg ttattatgaa gatacatacc
      541 ttgcagatta gtcagccccg ctaaagggct gaggtcactt atctggttat aactaagatc
      601 cagcgattga agatgagtca gaccagctaa aggactgagg tcacttatct ggttatcata
      661 aagaaaaatt aaattcagat tagtaagacc tgctaaagga ctgagatcac ttataaggtt
      721 agaactaaga cttagtcgtt gcagattaat tagatattga gcaccttcta tactgacaat
      781 atttcgccac caagcagata aagttccagt taatccattc aaatcagcta ttgttggctg
      841 ataatcagag ggctgaccca atgcctcatt aattaccatt ctaaaattat catcaggaat
      901 ataggcaata ggactggtaa ctacatattc aacaggaact gtgataagat gtgaaacagg
      961 atcattggat gagagttgca gttcatagtt gtaggtgcca agtgaatccc caggaataaa
     1021 attcaggata cagataaccg aagaattggg ttctatagtt ccgaaggaag gagaaaggtt
     1081 aatttccggc atttgggatg ttcctt
//
LOCUS       DAOQWT010000561         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_177542_length_1106_cov_2.855376, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000561 DAOQWT010000000
VERSION     DAOQWT010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177542_length_1106_cov_2.855376"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1106)
                     /locus_tag="PKJ95_08700"
     CDS             complement(<1..>1106)
                     /locus_tag="PKJ95_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66354.1"
                     /translation="TEGNVIIVELPDEEDMAEMRFNDYFIYDYTSKNYKKKPIDVFLS
                     KMLHKYNQRDKEIIQGFNKSIFSGFTISAVLPGYYFIAKDVCSGKEYKVRKNRATFQF
                     KKDDYFIGRILPFESDYVLSGINLFLPDEFSYLAKRSWRDLPMQVSDHINPLMIEREI
                     LQRSKSKRIEDENVEYYTQEPIEIIEKKLRNFLKKRMGKKAPSIKSLRKKINRITEPT
                     PLIEELLREMHISSQEEFIKFQQLFNDFWNRSPRDEFQGKSPEEVASESMGPVENELL
                     NEFMKYAKKNIDITKFSNKDEMEQTIRSFQEKWLNEPQEKLNGKTPFQVMREERERIH
                     SPRKGFPINFQVNPINLRQEDHFHFNNINEKDSP"
BASE COUNT          297 a          201 c          174 g          434 t
ORIGIN      
        1 tggagaatct ttctcgttaa tattgttaaa atgaaaatgg tcttcttgcc tgagattgat
       61 aggattcacc tgaaaattga taggaaaacc ctttcgggga ctgtgtattc tttctctttc
      121 ttccctcatt acctgaaaag gtgttttccc gttgagtttt tcctggggtt cattaagcca
      181 tttttcttga aaggacctaa ttgtctgttc catttcgtct ttattggaga atttagttat
      241 atcaatattc ttttttgcat atttcatgaa ttcatttaac agctcatttt ccacgggacc
      301 catactttct gatgccactt cttctggtga tttgccctgg aattcatctc ggggagaacg
      361 gttccaaaag tcgttaaaga gttgttgaaa tttaataaat tcttcctgtg aagaaatatg
      421 catttccctt aacagttctt cgataagagg agttggctct gtaatcctat ttatcttttt
      481 tcttaaactt ttaatagaag gagctttttt gcccattcgc ttcttcagaa aatttcttaa
      541 ttttttctct atgatttcta tgggttcctg agtatagtat tctacattct catcttctat
      601 ccttttgctt ttacttcgtt gaaggatttc tctttcaatc attaaagggt tgatatggtc
      661 ggatacctgc ataggcaaat ctctccagga tctttttgct aagtaagaaa attcatcggg
      721 tagaaatagg ttgataccag acaggacgta atccgattca aagggtaaaa tccttccaat
      781 aaaataatca tcttttttaa attgaaaagt agctcgattt tttcttactt tatattcctt
      841 tcctgagcac acatcttttg ctataaaata atatcccggc aatactgcac taatggtaaa
      901 gccactgaaa atacttttat taaatccctg aattatttct ttatctcttt gattatattt
      961 gtgcagcatt ttagaaagga acacatctat gggcttcttt ttataattct ttgaggtata
     1021 gtcataaata aaatagtcgt tgaacctcat ttctgccata tcttcttcat ctggtaattc
     1081 aacgataatg acattccctt ctgttt
//
LOCUS       DAOQWT010000562         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_180289_length_1096_cov_9.229587, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000562 DAOQWT010000000
VERSION     DAOQWT010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180289_length_1096_cov_9.229587"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1096)
                     /locus_tag="PKJ95_08705"
     CDS             complement(<1..>1096)
                     /locus_tag="PKJ95_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR66355.1"
                     /translation="SPLAGLTNLQELYLGYNQISDLSPLLGLTNLQELALHNNQISDL
                     SPLAGLTNMQWLHLNYNQISNLNPLVGLTNLHYLYLENNQISDLNPLAGLNNLQDLWL
                     SYNQISDLSPLAGLTNLRWLHLDYNQISDLSPLAGLTNLQVLYLDYNQISDLSPLAVM
                     SNLQYLWLVYNQISDLSPLAGLTNLKGLYLQYNQISDLSPLEGLSNLVDLWLFYNQIS
                     DLSPLAGLTNLRWLHLDYNQISDLSPLEGLTNLYNLYLENNQISDLSPLEDLTYLQYL
                     ELNNNQISDISPLEGLTNLLELYLNYNPISYESMLLSQNWSLPWLTNEYNPLSPCYPD
                     PNRDAVNVSTGSDLSWQGNYDNSQDVFYEVW"
BASE COUNT          356 a          208 c          217 g          315 t
ORIGIN      
        1 accagacctc atagaaaaca tcctggctat tatcataatt cccctgccag gataaatcac
       61 tgcctgtaga tacatttact gcatctctat ttggatcagg ataacaaggc gaaaggggat
      121 tatattcatt ggttaaccaa ggtaaagacc aattttgaga aagaagcatt gactcataag
      181 aaataggatt atagttaaga taaagctcta gcaaattagt cagcccctct aaaggactga
      241 tgtcacttat ctggttatta ttaagttcca gatattgcag ataagtcaga tcctctaaag
      301 gactgaggtc acttatctgg ttattttcaa gatatagatt atacagatta gtcagcccct
      361 ctaaaggact gaggtcactt atctggttat aatcaagatg tagccatcgc agattagtca
      421 gccccgctaa aggactgagg tcacttatct ggttataaaa tagccaaaga tctaccagat
      481 tagaaagacc ttctaaagga cttaggtcac ttatctggtt atattgaaga tatagccctt
      541 tcagattagt aagacccgct aaaggactga ggtcacttat ctggttataa acaagccaca
      601 gatattgcag attagacata acagctaagg gactgaggtc acttatctgg ttataatcaa
      661 gatacagcac ttgcagatta gtcagaccag ctaagggact gaggtcactt atctggttat
      721 aatcaagatg tagccatcgc agattagtca gccccgctaa aggactgagg tcacttatct
      781 ggttataaga aagccacaga tcttgcagat tattcaatcc cgctaaagga ttgaggtcac
      841 ttatctgatt attttcaaga tatagataat gaagattagt tagccccact aaaggattta
      901 ggttacttat ctggttataa ttaagatgta gccattgcat attagtcagc cccgctaaag
      961 gactgaggtc acttatctgg ttattatgaa gagctagttc ttgcagatta gtcagcccca
     1021 ataaaggact gagatcactt atctggttat aaccaagata tagctcttgt agattagtca
     1081 gccccgctaa aggact
//
LOCUS       DAOQWT010000563         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_180584_length_1095_cov_3.750000, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000563 DAOQWT010000000
VERSION     DAOQWT010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180584_length_1095_cov_3.750000"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKJ95_08710"
     CDS             <1..716
                     /locus_tag="PKJ95_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNR66356.1"
                     /translation="NLNFGNPEKEEIFWQLEEAVKGISKAAKEMGIPVISGNVSLNNE
                     SNKEAIFPTPVIGMAGIIEDFSKICTLSFKNEHDLVVLIGENKEELGGSEYLKVFHNL
                     ERGLPPQVDLNLEKSVQNVCRRCIDLGILSSAHDCSEGGLLIALSECCSAGKVGIKVD
                     KRVKTRNDALLFGESQSCIIVSLPQEKLSLLKDIALKYQAPIEVLGEVGGDSLKFGNL
                     VNLKIEQILEARERNFDNA"
     gene            709..>1095
                     /locus_tag="PKJ95_08715"
     CDS             709..>1095
                     /locus_tag="PKJ95_08715"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205037.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNR66357.1"
                     /translation="MHEECGVFGVYLKDVKANIAQLSYLGLLALQHRGQESAGIAVAG
                     GENILIYRDLGLVTEVFKKDILGTLHGDAAIGHVRYSSAGSNSWEDSQPILHHFSGGS
                     FALAYNGNLVNQSELSSMLAIHYKNSN"
BASE COUNT          346 a          158 c          252 g          339 t
ORIGIN      
        1 ataatcttaa ctttggaaac cccgaaaaag aggaaatatt ctggcagtta gaagaagcag
       61 ttaaagggat tagcaaagcc gctaaggaaa tggggatccc ggttatcagt ggtaatgtca
      121 gtctcaacaa cgaaagtaat aaagaggcta ttttccccac tcctgttatt ggtatggcgg
      181 gaattattga agatttttct aaaatctgca ctctttcctt taaaaatgag cacgatttag
      241 tggtattaat tggagaaaat aaagaggagt taggaggaag tgaatactta aaagtattcc
      301 ataatttaga aagaggctta cctccccaag ttgatttaaa tttggaaaag tctgttcaaa
      361 atgtctgccg gagatgtatt gatctgggga tattatcttc tgcccatgac tgttctgagg
      421 gaggtttatt aattgccctt tcagaatgct gttcagcagg gaaagtggga ataaaagttg
      481 ataaaagagt aaaaactaga aatgatgctc tcctttttgg tgaatctcaa tcctgtatta
      541 ttgtttctct tccacaggaa aaactatctt tgctaaaaga tattgccctt aaatatcagg
      601 cacctataga ggtattggga gaagtggggg gagatagcct gaagtttggt aatctggtta
      661 atttaaaaat cgaacaaatt ttagaagccc gggaaaggaa ttttgataat gcatgaagag
      721 tgtggcgttt ttggtgttta ccttaaagat gtaaaagcta atatagccca gttaagttat
      781 ttggggcttc ttgctcttca gcatagggga caggagagtg ccggaatagc cgttgctggt
      841 ggagaaaata tattgattta tagagatttg ggtctggtta ctgaagtttt taagaaagat
      901 atattaggta cccttcatgg agatgcagct atcggtcatg ttagatattc ttctgcaggt
      961 tctaatagct gggaagatag tcaaccaata ttacaccatt tttcaggtgg ttcttttgcc
     1021 ctggcttata acggaaattt agtcaatcag agtgagttaa gttcaatgtt ggccatacat
     1081 tacaaaaatt caaat
//
LOCUS       DAOQWT010000564         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_182381_length_1089_cov_2.550290, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000564 DAOQWT010000000
VERSION     DAOQWT010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182381_length_1089_cov_2.550290"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..>1089)
                     /gene="hemW"
                     /locus_tag="PKJ95_08720"
     CDS             complement(54..>1089)
                     /gene="hemW"
                     /locus_tag="PKJ95_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNR66358.1"
                     /translation="YVDALKKEMEIYSQKIRDVEIISIYVGGGTPTVLTGEQLSEILS
                     SCYHNFQIAKKVESTVEANPGTIDKEKMERLLQSGVNRISLGAQSFNNKALKKLGRIH
                     TKEDNIAAYQLARSAGFKNINVDIMFGLPGQSKKEFENVLTELMLLKPEHISLYALSV
                     ESGTPLDNTIKTGKTRLPCDDFCNKLFLSASDYFSEYGYEHYEISNFAQSAKRCIHNE
                     LYWKNRAYLSIGAGATSYIDNKRYQNYQELNQYIYLLENGILPIRYQEVLPLKERMAE
                     TIILNLRMMEGLEKKAFHSLFNITVEDIYYQSLHNLKEQGLLTESNTHYFLTKKGILL
                     ANNVFMEFLD"
BASE COUNT          340 a          218 c          155 g          376 t
ORIGIN      
        1 tattgacata cttctgccag aatatcggtt cactacaaga gaatataaaa gattcaatcc
       61 aaaaattcca taaagacatt attagccagt aaaatacctt ttttagtaag aaaataatgg
      121 gtattgctct ctgttaacag accctgttct tttaagttat gtaatgattg gtagtatata
      181 tcctccaccg tgatattaaa aagtgaatga aaagctttct tctccagccc ttccatcatc
      241 cttaaattta aaattatagt ttcagccatc ctttctttta gcggaaggac ctcctggtat
      301 ctaattggta aaataccatt ttcaagaaga taaatatatt gattcaattc ctgataattc
      361 tgataacgtt tgttatcaat ataggaagtc gctccggcgc caatactcaa atatgctcga
      421 tttttccaat atagttcatt atgtatgcat ctttttgcag attgggcaaa attagatatt
      481 tcataatgtt cataaccgta ctcagaaaaa taatcagaag cacttaaaaa tagtttattg
      541 caaaaatcat cgcaaggaag tcttgtcttg cctgttttta tagtattatc aagaggagtt
      601 cccgattcaa cagaaagggc ataaagggat atatgctcag gcttaagtag cattagttct
      661 gtcaaaacat tttcaaattc ttttttactc tgccctggca aaccaaacat aatatcaaca
      721 ttgatattct taaatcccgc tgaccttgcc agctgatagg cagctatatt atcctcttta
      781 gtatgtattc ttcccaactt ctttaatgct ttattgttaa aagactgagc tcccaggcta
      841 attctattta ccccggattg taacaatctt tccattttct ccttatctat agtgccagga
      901 tttgcctcaa ctgtgctttc aactttttta gcaatttgaa aattatggta gcaggaagac
      961 aatatttcac ttaattgctc tccggtcaac accgttggag tgccaccccc tacatagatt
     1021 gaaattatct ccacatccct aatcttttga ctatagattt ccatttcctt ctttaaagca
     1081 tcaacatac
//
LOCUS       DAOQWT010000565         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_182452_length_1089_cov_2.095745, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000565 DAOQWT010000000
VERSION     DAOQWT010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182452_length_1089_cov_2.095745"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKJ95_08725"
     CDS             complement(<1..262)
                     /locus_tag="PKJ95_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66359.1"
                     /translation="MKILFIENFQYVHATYKYPNIPLGLLSLATILKEKDYDVELINF
                     NYLFLNNIIDYDFDTAKKFDIMAGYIMNKNPDIVGFTALCNTF"
     gene            662..874
                     /locus_tag="PKJ95_08730"
     CDS             662..874
                     /locus_tag="PKJ95_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013320546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNR66360.1"
                     /translation="MKFIIKLEKAEEGGFNVSVPALDGCYTQGDTEEEAIENAKEAII
                     CYMEGLQKINQINITSSIMKEVEVSL"
     gene            871..1086
                     /locus_tag="PKJ95_08735"
     CDS             871..1086
                     /locus_tag="PKJ95_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002766803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNR66361.1"
                     /translation="MSKTFSGKDIVKALIKSGFIIDHQRGSHIFLHNPDKKISIIVPN
                     HKEIKKGTLNSIIKKASITIQELKTLI"
BASE COUNT          391 a          167 c          174 g          357 t
ORIGIN      
        1 gaaaggtatt gcatagagca gtaaacccta ctatatcagg atttttattc attatataac
       61 cggccataat gtcaaatttc ttcgccgtat caaagtcata gtctattatg ttatttaaaa
      121 ataagtaatt gaagtttatt aattcaacat cataatcttt ttctttcaga attgtagcca
      181 gactcaataa tcccagtggt atattgggat atttataggt agcatggaca tattgaaaat
      241 tctctataaa taatattttc ataaattatt cctcccagaa attttttaga gtgttgtaat
      301 gtttgcgtaa atatctaagt accgaaacaa ataagtcagt atccatcgta aaaccctgtc
      361 gcaggacgga ctcttccgaa aaatctataa tcctcgtgaa agaaaatctg tctttgcaga
      421 catttactat tttgcgtcta tttaatttac aactggctat taatacggta tatatttttc
      481 tccagagccc gtcctgcgac aggtgtctac ttgattattt ttttggtacc cttttacctg
      541 gaacggtaat tagagactat ctgaaaagta gagagtcatc tttcacagac tctgcttgaa
      601 cagatctaaa aggacttttc ggacaggttc taaataatat cacaggaagg tgtaatatta
      661 tatgaaattt ataattaaat tagaaaaagc tgaagaaggc ggttttaatg tatcagttcc
      721 tgctcttgat ggttgttata ctcagggaga tacagaagaa gaagctatag aaaatgctaa
      781 agaagctatc atttgctata tggaaggtct tcaaaaaata aatcagatta atattacctc
      841 cagtattatg aaagaggtag aagtttcatt atgagcaaga ccttttcagg caaagacata
      901 gtcaaagcac ttattaaatc cggttttatt atagaccatc aaagaggaag ccatattttt
      961 cttcataatc cagataaaaa aatctcaata attgttccaa atcataagga aattaagaaa
     1021 ggtacattaa attcgataat aaaaaaagca tcaataacta ttcaggaatt aaagacttta
     1081 atatagtta
//
LOCUS       DAOQWT010000566         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_183489_length_1085_cov_3.287379, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000566 DAOQWT010000000
VERSION     DAOQWT010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183489_length_1085_cov_3.287379"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKJ95_08740"
     CDS             complement(<1..274)
                     /locus_tag="PKJ95_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HNR66362.1"
                     /translation="MSEKEKFQIVTVNRKAWHDYHILESFEAGLVLKGTEVKSIRNSQ
                     VSIKESYAQLKGNDLYIVNMHIAPYQFGNRFNLEPKRDRKLLLNKRE"
     gene            complement(379..>1085)
                     /locus_tag="PKJ95_08745"
     CDS             complement(379..>1085)
                     /locus_tag="PKJ95_08745"
                     /inference="COORDINATES: protein motif:HMM:NF015446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HNR66363.1"
                     /translation="KSISDIVTSGLDTVAIRIPDHKVALGLIKEARRPIAAPSANLFS
                     HTSPTTAWHVISDLKGSIDIIIDSGETLIGLESTVLDVTTSPLQILRLGGITFESLKK
                     IIPDIIIADDHIKIKKAPGMLSRHYSPLAKLILVKDKDEKMIEKIRKIALQHRNKGKK
                     VGVIACQENREKYSGFIVKVLGEAKDLNSCARNLYAQLRILDELKCDIIISENFEDMG
                     IGRAIMDRLKRASRNC"
BASE COUNT          334 a          224 c          144 g          383 t
ORIGIN      
        1 attctctctt atttaagaga agttttctat ctctctttgg ctccagatta aatctatttc
       61 caaactgata tggagcaata tgcatattaa caatatataa atcgtttccc tttaactgag
      121 cataactttc tttaatactt acctgactat tcctgattga cttaacttcg gttcctttta
      181 aaacaagacc agcttcgaag ctttccaaga tatggtaatc atgccatgct ttccggttta
      241 cagtaacaat ttggaatttt tctttctcag acatccaatt atccctttga accaattaaa
      301 ttataacata tttccgcttt ttttactatt tatagtatcc aaaaactaaa taaaggtgtt
      361 aaaacataac taaataaatt aacaatttct tgatgccctc tttaagcggt ccataatggc
      421 tcttcctatt cccatatctt caaaattttc agaaattatt atatcacatt ttaattcatc
      481 caaaattctc agctgagcat acaaattacg ggcacaggaa ttaaggtctt tagcttcccc
      541 aagcacttta actataaacc cggaatattt ttcccgattt tcctgacagg caatcactcc
      601 aactttttta cctttatttc tatgctgtaa agctatcttg cgaatctttt caatcatctt
      661 ctcatcctta tcttttacca gtattagttt agctaaagga gaatagtgcc tacttagcat
      721 tcctggtgct ttttttattt taatatggtc atcggcaatg atgatatctg gaattatctt
      781 ttttaaactt tcaaaagtaa taccacctaa tctcaatatc tgtaaagggg acgtagtgac
      841 atccaatact gttgattcaa gccctattaa ggtttcacca ctatcaataa ttatatctat
      901 actacccttt aaatcagaaa ttacatgcca ggcagtggtg ggactggtat gactaaacaa
      961 attagcgctg ggagccgcaa tgggtcgccg agcttcttta atcaatccca atgcaacctt
     1021 atgatcaggg atacgtatag caacagtatc taacccggaa gtaactatat ctgaaattga
     1081 tttac
//
LOCUS       DAOQWT010000567         1084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_183834_length_1084_cov_2.473275, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000567 DAOQWT010000000
VERSION     DAOQWT010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183834_length_1084_cov_2.473275"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PKJ95_08750"
     CDS             complement(<1..544)
                     /locus_tag="PKJ95_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HNR66364.1"
                     /translation="MYSSRKKLLLVLTLVFFLSLMTLSVLAQGKWKIGIMTNTVTQNE
                     EEYRAAQHVLAKYGPEHIIHMTFPDKFMDEQETTIANLVSMASDPDLKALVIVQAVPG
                     TSPGIDSVKELRDDLLIIAGQPQEDPPVISTRADLVLQMDEMGMGVGIPTQAKFLGAK
                     VLVHYSFPRHMSYPLLSGRRE"
BASE COUNT          319 a          199 c          187 g          379 t
ORIGIN      
        1 attcccttct gcctgacaaa agaggataag acatatgtct ggggaaggag tagtgaacca
       61 gaacttttgc tcccaggaac tttgcctgtg ttggaattcc tacccccatt cccatctcat
      121 ccatctgtaa taccagatca gctctggttg aaattaccgg cgggtcttcc tggggctgac
      181 cggctatgat aagcagatca tccctgagct ctttcacgct atctattcca ggagaggtac
      241 cgggaactgc ctgaacaatg accaaagctt ttaaatcggg gtctgaagcc atactcacca
      301 ggttagcgat ggtggtttcc tgctcatcca taaatttatc agggaaggtc atatggatta
      361 tatgttccgg accatattta gccagtacat gctgtgctgc cctgtactcc tcctcgtttt
      421 gagtaacagt gttggtcatt atacctattt tccacttacc ctgtgccagc actgaaaggg
      481 tcatcaatga taagaagaat actaatgtta aaaccagtaa taattttttt cttgagctat
      541 acattcatct cctccataat taaattttga ttttttgtga gcctttcctt ttggatgaca
      601 aagcctcaag cctgaatggt tagttcaata aagtaaatag taaagtataa aatttcaccc
      661 ccttatataa tttttatttt tgttgaacac aggtataaaa aagaaattta aatgtagaga
      721 ctataccggt atgtctttat ttattcttac aaatgaaatg atttatcagg aatgtttttt
      781 ctgcattctt aagggaacag ctttttatct cttatatttt aatattttca tttcggtttt
      841 ctaattggca attacatctg tttttgccag taagccgttt taaaatatat aaaatatagt
      901 taatttacaa aaaatgccgc ttccaagaat aaaaataaag atgctaaata ggcttttatg
      961 tagattgttt aatgtgcatt tttaattata gacaaataat catttgcttg tcaatgatta
     1021 tagtaattat gttagtgtca acaacttgtg ggtatttttt ccttacctca taatctttgg
     1081 aaat
//
LOCUS       DAOQWT010000568         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_185766_length_1078_cov_1.690127, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000568 DAOQWT010000000
VERSION     DAOQWT010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185766_length_1078_cov_1.690127"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..133
                     /locus_tag="PKJ95_08755"
     CDS             5..133
                     /locus_tag="PKJ95_08755"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66365.1"
                     /translation="MSVPSRYIHSVVEMCHIDDIEASISLLVSFLENIHLADFYNL"
     gene            143..823
                     /locus_tag="PKJ95_08760"
     CDS             143..823
                     /locus_tag="PKJ95_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HNR66366.1"
                     /translation="MMVNQKNSNELYSMHQVLLEFYGSLHWWPGESSLEIMVGAILTQ
                     NTNWQNVSKAIEKMKEEGLLEVTALFHMDEKSLGKIIKSCGYYNLKARRLKNFINFFY
                     RNYGGSVERLFSVDWKILREELLKINGIGPETADSILLYAGNKPIFVVDAYTRRIFHR
                     HNYFSFKASYQEIQHFFMVHLPQDHSVYNEFHAQLVMLGKDFCNKNNPLCSQCPLSPF
                     LNKKTSHS"
BASE COUNT          348 a          193 c          202 g          335 t
ORIGIN      
        1 tggtatctct gtaccgtccc gttatatcca ttcagtagtg gagatgtgtc atatagatga
       61 tatagaagca tctatctccc tgctggtctc ttttctggag aatatacatt tagctgattt
      121 ttataatttg taattctcgg gaatgatggt taatcagaag aacagcaatg aactgtactc
      181 aatgcaccag gtccttttgg agttttatgg ttccctgcat tggtggccag gcgagtcatc
      241 actggaaata atggtaggag ccatcttaac ccagaataca aactggcaga atgtaagcaa
      301 agccatagaa aaaatgaaag aagaagggct gcttgaagta acagcccttt ttcatatgga
      361 tgaaaaatct cttggtaaaa taattaaatc ctgcggctat tataatctaa aagccagacg
      421 cttgaagaat ttcatcaatt ttttctatcg caattatggt ggttcagtgg aaagactatt
      481 cagtgttgac tggaagattt taagagagga attgctaaaa ataaatggta taggacctga
      541 aactgcggat agtattctgc tctatgctgg taacaaacca atttttgtgg tagatgctta
      601 tacccgaagg attttccacc gacataacta tttttctttt aaagcaagtt atcaggaaat
      661 tcaacacttc tttatggtac atctacctca ggatcactct gtgtacaacg aattccatgc
      721 ccagctggtt atgctgggga aagatttctg caataaaaat aaccctctct gttcacaatg
      781 ccctttatct ccttttttaa acaagaagac ttcccattct tagcattgag ctttttgtct
      841 ctatcagata aaatattaac acagtactat atagataaat atttattaca tttatatatt
      901 acatattact gataacagtt aactgtatgg caatggtaat ggtaaaggtc agatatggat
      961 gataaaatat ttcagaatga aattctaaag aaagaagaca gagcactctc cccctttgct
     1021 actaaaaata ttgatgccca tcgaaaagta gcgatagccc acccgttgag aggagcct
//
LOCUS       DAOQWT010000569         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_186245_length_1076_cov_2.333007, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000569 DAOQWT010000000
VERSION     DAOQWT010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186245_length_1076_cov_2.333007"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /gene="hypE"
                     /locus_tag="PKJ95_08765"
     CDS             <1..973
                     /gene="hypE"
                     /locus_tag="PKJ95_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNR66367.1"
                     /translation="GKLSNQLIRSLFLKYFNNPFLNQLHDGAILPPSDKKIAFSTDSY
                     TVSPIFFNGGDIGKLAIHGTVNDLAMCGAIPKYLSVSLIIEEGTSMYALEQITSSMAE
                     AAQQTDILIVTGDTKVVNKGDVDGIFVNTTGIGYIDLDVDINPARAQVNDFILINGPI
                     GNHGISILASRENLELEESIPSDTAPLADLVQQMLSITREIHVLRDPTRGGLATALNE
                     IAQSSHVEIEIGEVKIPMDEAVKDTCQILGYDPLYLANEGKLIAFVPEQYAEKVLEKM
                     KKSCYGEQAAIIGRVVARGKAEVSLLTEIGGKRIVGMLSGEQLPRIC"
BASE COUNT          339 a          183 c          239 g          315 t
ORIGIN      
        1 agggaaatta agcaatcagc tcatccgctc ccttttttta aaatatttta ataatccttt
       61 cttaaatcag cttcatgatg gagcaatatt accaccatca gataaaaaaa ttgccttttc
      121 cacagattca tacactgtga gcccaatatt ttttaatgga ggagatatag gtaaactggc
      181 aatacatggt actgtaaatg atttagctat gtgtggggct attccaaaat atcttagtgt
      241 atcactgatt attgaagagg gtacctcaat gtatgccctg gaacagatta cctccagtat
      301 ggctgaggcg gcacaacaaa ccgacattct aattgttacc ggagatacca aggtggtaaa
      361 taagggtgat gttgatggaa tatttgtaaa taccacaggt attggttata ttgatttaga
      421 tgttgatatt aatcccgcca gggcacaggt caacgatttc attctgatca acggtcccat
      481 cggaaatcat ggtatcagca tacttgcctc ccgcgaaaat ttggagctgg aagagagtat
      541 accgagtgat actgccccac tggcagattt ggttcaacag atgttgtcca taaccagaga
      601 gatacatgtg ctgcgtgatc ctaccagggg aggtcttgct actgctttaa atgaaattgc
      661 ccaatcttct catgttgaaa tagaaattgg ggaagtaaaa atccctatgg acgaggctgt
      721 aaaagatacc tgtcaaattt taggttatga ccctctctat ttagccaatg aggggaaatt
      781 gattgccttt gtgccagagc aatatgcaga aaaggtgtta gaaaagatga aaaaaagttg
      841 ttatggagaa caggcggcta ttatagggag agtagttgcc cgggggaagg cggaggtatc
      901 tcttctaact gaaatcggtg gaaagagaat agtgggaatg cttagcggag agcagcttcc
      961 cagaatatgt tgaaaatctc aattgctata taaataattg agattgaaat ttcaggtcaa
     1021 ataaattaag ataagctgtt aactgttctg tcttttaaag tctttactat ttattc
//
LOCUS       DAOQWT010000570         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_186970_length_1073_cov_7.411591, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000570 DAOQWT010000000
VERSION     DAOQWT010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186970_length_1073_cov_7.411591"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1073)
                     /gene="rffA"
                     /locus_tag="PKJ95_08770"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(<1..>1073)
                     /gene="rffA"
                     /locus_tag="PKJ95_08770"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNR66368.1"
                     /translation="EYIQDAINRGHISGNGYYTKKCQNFFQENYGFLKCLLTTSCTDA
                     LEMAAILINTQPGDEIILPSFTFVSTANAFALRGAKLIFCDSRADHPGIDEDKIEDLI
                     TEKTKAIVPVHYAGVACDMDKIMALAEKYNLFVIEDAAQAIDSYYKGKPLGGIGHLGC
                     FSFHETKNISSGEGGMLVVNDDRFIERSEIIWEKGTNRQAFFRGEIDKYGWVDIGSSF
                     LPSDMIAAFLWAQLEHIYEIQTRRLEIWNQYNETLKPIANKAEIRLPFIPDYATNNAH
                     MYYIVAEGRGQRAEGKGQRDRIIKKLKDNGVQAVIHYQSLHNSKFYSSQSMGRECPQS
                     DYYSLNLLRLPLFIGLDTTITKN"
BASE COUNT          341 a          220 c          156 g          356 t
ORIGIN      
        1 tatttttagt tatagtagta tctaacccta taaataaagg caatcttaac aggtttaaag
       61 agtaataatc tgattggggg cattctcttc ccattgattg agatgaataa aatttggagt
      121 tatgtaaact ctggtaatga ataactgcct gcacaccatt atcttttaac tttttaataa
      181 ttctatctct ctgcccttta ccctctgccc tctgccctct gccctctgct acaatataat
      241 acatatgtgc attattagtt gcataatcag gtataaaagg aagacggatt tcagctttat
      301 ttgctattgg tttaagggtc tcattatatt gattccaaat ttctaaacgc ctggtttgaa
      361 tttcgtaaat atgttccagt tgagcccata aaaaagcagc aatcatatca gaaggcaaaa
      421 aagaagaacc aatatctacc caaccgtatt tatcaatttc tccccgaaaa aatgcctgtc
      481 tatttgttcc tttttcccaa ataatttcac ttctttcaat aaacctgtca tcattaacca
      541 ctaacattcc accctcacca gaagaaatgt tcttagtttc atgaaaggaa aagcatccca
      601 gatgacctat tcctcctaaa ggttttcctt tataataact atctattgct tgagctgcat
      661 cttcaataac aaataaatta tatttttcag ctaaagccat aattttatcc atatcacaag
      721 ctacacctgc atagtgaacc ggaacaatag ctttggtttt ttccgtaatt aagtcctcaa
      781 ttttatcttc atcaattcca ggatgatcag ctctgctatc acaaaaaata agttttgccc
      841 ctcttaaagc aaaagcatta gctgtagaaa caaaagtgaa ggaaggtaaa ataatctcgt
      901 ctccgggttg agtatttatt aaaattgcag ccatctccag ggcatctgtg caagaagttg
      961 ttaaaaggca ttttaagaaa ccatagtttt cctgaaaaaa gttctggcat ttcttggtgt
     1021 aatacccgtt acccgaaata tgtccccggt ttatagcatc ctggatatat tcc
//
LOCUS       DAOQWT010000571         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_192948_length_1054_cov_2.899900, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000571 DAOQWT010000000
VERSION     DAOQWT010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192948_length_1054_cov_2.899900"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..959)
                     /locus_tag="PKJ95_08775"
     CDS             complement(57..959)
                     /locus_tag="PKJ95_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNR66369.1"
                     /translation="MITSFQQLFREITIYGKQRIVIAGGEDPEALKAVKASFENGFGK
                     GVLVGNKNKIEDSLMELGYQDRAFIENIVSAREEEKAYIAIEEVKNGGILLKGKINTA
                     SLMSAVLHKEKGLRTDDFISNVFVFEDRREKQSRLILLSDGGINIKPDLKAMVAIIKN
                     AVKVAHKLGIENPKVALLAAVETVNPDMEETLQASIITRMYQRGQIENCIIDGPLALD
                     NAISEFAAEKKGIDSPVAGKADILIVPNIACGNIFGKSIMYYTDFPEGNVVVGARVPI
                     MIPSRADKSEGKLNSIALSILCSR"
BASE COUNT          292 a          256 c          152 g          354 t
ORIGIN      
        1 cagtcactta tgttgattta gtcactcgta agtaggaaaa gctcttttcc ttactgttat
       61 ctgctgcaaa gaatagagag ggctatggaa tttaatttgc cttcactctt atcagcccta
      121 gagggtatca taatcggtac cctggcaccc accaccacat taccttccgg gaagtcagta
      181 taatacatta tacttttacc aaaaatatta ccacaggcaa tattaggtac tatgagaata
      241 tcggcttttc ccgctaccgg ggagtcaatt cctttcttct cagcagcaaa ctctgaaata
      301 gcattatcta aagccagcgg tccatcaatt atgcaatttt ctatctggcc cctctgatac
      361 attctggtta taatacttgc ctgcagagtc tcctccatat caggatttac tgtctctact
      421 gcagccagta acgctacttt ggggttctct atacctaatt tgtgggcaac cttcaccgca
      481 tttttaataa tagccaccat tgcctttaaa tcaggtttaa tatttattcc cccatcactt
      541 aacaatatta aacgggactg cttttctctc ctgtcttcaa aaacaaaaac attgctgata
      601 aaatcatctg ttcttaaacc cttttcttta tggagaactg cactcatcag ggaagcggta
      661 ttaatctttc cttttaacag gataccacca ttttttacct cttctatggc tatgtaggcc
      721 ttttcttctt cccgggcaga gacaatgttt tcaataaaag ctctatcctg gtatcctaat
      781 tccatcagag aatcttcaat tttattctta ttacctacca gcactccttt accaaaacca
      841 ttttcaaaac tggccttgac cgccttgagt gcttccgggt cttctccgcc ggcaattaca
      901 attctctgct taccatagat agtaatttcc cgaaaaagct gctgaaaact ggttatcata
      961 ttcgatctcc ctatagttaa gctgcaatat gtacagcctt tacaatccta atacaatctc
     1021 tttactattt aaatatttta caaaatatta tttc
//
LOCUS       DAOQWT010000572         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_193639_length_1052_cov_2.484453, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000572 DAOQWT010000000
VERSION     DAOQWT010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193639_length_1052_cov_2.484453"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /gene="prs"
                     /locus_tag="PKJ95_08780"
     CDS             <1..467
                     /gene="prs"
                     /locus_tag="PKJ95_08780"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNR66370.1"
                     /translation="KVVVVSPDVGGTARARNFAGYLDKPIAIIDKYRSSETSVEIMHI
                     VGNVKNRTAIIVDDMIDTAGSIIKGSQALMEAGAKEVFVCATHPIFANPARERLEKQW
                     QERLFQEIVVTNTIPVPPEKQLPCIKVLSVSKLFAEAIKRIHFNQSISALFR"
     gene            619..>1052
                     /locus_tag="PKJ95_08785"
     CDS             619..>1052
                     /locus_tag="PKJ95_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNR66371.1"
                     /translation="MKRIQLQVETREKSGKEESKRLKNNGYVPGIFYSPHDKDNILLK
                     IPEKELFRFLSDKKHAHGIIDLKIKTDDKKEITRLALMKDFQYNSLKRKINHFDFYGV
                     TLKEKVTLELPLILEGEPKGIKEGGILDISLREVEVECLPADA"
BASE COUNT          377 a          161 c          210 g          304 t
ORIGIN      
        1 caaaagtggt agtggtgtct cccgatgtag ggggaacagc acgagccagg aattttgccg
       61 gctatttaga taagcccata gctattattg acaaatacag gtcttctgaa accagtgttg
      121 aaattatgca tatagttggg aatgtcaaaa acagaactgc tattatagtg gatgatatga
      181 ttgatactgc gggctctatt ataaagggaa gtcaggcgtt aatggaagcc ggagctaaag
      241 aggtgtttgt ttgcgctaca catcccattt ttgccaatcc agctcgggag cgcctggaga
      301 agcaatggca ggaacgttta tttcaggaga tagtagttac caataccatc ccggttcctc
      361 ctgaaaaaca gctaccctgt ataaaagtat tatcagtttc caaactattt gccgaagcca
      421 tcaagcgcat tcatttcaat caatccatta gtgccttatt tagatgagat ttagataaga
      481 gtttaagttc aaatggttca aataaaatat ttaaaaaatt actttaattg tattgtactt
      541 atatagtata ataataattt gatattaatt aaaaataata aatttgtgca aaaagaattg
      601 ttgcgaggtg aacaaattat gaaacgaatt caactacagg tagaaaccag agagaaaagc
      661 gggaaagaag agagtaaaag acttaaaaat aatggatatg ttccaggtat cttttattct
      721 cctcatgata aagataatat tctattgaaa attccagaaa aagaactctt ccgtttttta
      781 tctgataaaa aacatgccca tggcataatt gatttaaaga taaaaacaga tgataaaaaa
      841 gaaataactc gtttagcgct gatgaaagat tttcagtata actcattaaa aagaaaaatc
      901 aaccatttcg atttctacgg agttaccctg aaagaaaaag taactttgga actccctctt
      961 atcttggaag gagagcccaa aggtattaaa gaaggcggta ttctggatat atctttgcga
     1021 gaggtggaag tagaatgttt gcctgctgat gc
//
LOCUS       DAOQWT010000573         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_193878_length_1051_cov_3.496988, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000573 DAOQWT010000000
VERSION     DAOQWT010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193878_length_1051_cov_3.496988"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1051
                     /locus_tag="PKJ95_08790"
     CDS             <1..>1051
                     /locus_tag="PKJ95_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR66372.1"
                     /translation="RLYYSKAEKTVRYDFGKTLLELRATGQTVFPLSIHTSGEFITWE
                     NTLPIKEISPSELYKITGKIASACILSKFWYEGTRQDISLCLSKILIEANWSKEEISE
                     FFKAICTLSGDTDLKQRISGLYATGKKIEKGKPVYGIPKLKSLTSDRCVDKVIEWLQV
                     KSEENPEEKKRSKLQIFSAAEIMQEEYSPTRYIVEDILPEGLIVLAGRPKTCKSFLAL
                     NLSLSIAAGKPALKKLATEKTGVLYFALEDGKKRLDKRMKNILGEDWQSLLPKDLFFC
                     LKLNALDNGGFEDLEDALKENPGIGVIIIDTFAKIKPLSRAGNAYEADYKIAGQLQSW
                     AITKGIALVMIHHTR"
BASE COUNT          368 a          177 c          216 g          290 t
ORIGIN      
        1 acaggcttta ttactccaaa gcagagaaaa ctgtaaggta tgattttggt aaaacacttc
       61 tggaattacg tgccacaggt caaacggtat ttcctcttag tattcataca tcaggagaat
      121 tcattacatg ggaaaacact cttcctatta aagaaatatc accttcagag ttgtataaaa
      181 ttaccgggaa aattgcctcc gcctgtattc tttcaaaatt ttggtatgaa ggaaccaggc
      241 aagatatatc actctgtctt tcaaagatac ttatagaagc aaactggagt aaagaagaga
      301 tttcagaatt ctttaaagct atatgtactt tgtcaggaga cacagattta aaacaaagaa
      361 tttcggggtt atatgctact ggaaagaaaa ttgaaaaagg gaaacctgtg tacgggattc
      421 cgaaacttaa atctctcaca tctgatagat gtgttgataa agtcatagaa tggctccagg
      481 tgaagagtga agaaaatcct gaagaaaaga aacgaagtaa actgcagatt ttttccgcag
      541 cagagataat gcaggaagag tattccccta cgcgttatat agtagaagac attctccctg
      601 aagggcttat agttttagca ggaaggccaa agacatgtaa aagttttctt gctcttaatt
      661 tatctctttc tatagcagcc gggaaacctg cgcttaaaaa acttgcgaca gagaagaccg
      721 gtgttttata tttcgccttg gaagacggta aaaaaagact cgataaaaga atgaaaaata
      781 tccttggaga agactggcaa agccttttac caaaggattt atttttttgc ttaaaactca
      841 atgctctgga taatggagga tttgaagatc tggaggatgc tttaaaagaa aatccgggaa
      901 taggggttat aattattgac acttttgcaa aaataaaacc tctttccagg gccggtaatg
      961 cttatgaagc agattacaaa attgccggac agctccagtc atgggctatt acaaaaggga
     1021 tagctcttgt aatgatacat cacacccgga a
//
LOCUS       DAOQWT010000574         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_194890_length_1048_cov_3.357503, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000574 DAOQWT010000000
VERSION     DAOQWT010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194890_length_1048_cov_3.357503"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1010
                     /locus_tag="PKJ95_08795"
     CDS             117..1010
                     /locus_tag="PKJ95_08795"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNR66373.1"
                     /translation="MIAKIKSSRKWQLSILGIFIVIAIAAFWYFRANPVMPQMTIYFL
                     SEVNLPQQGDTVLIFAPHPDDETIACGGYINESVKRGAEVHIVLVTDGNRRNLQVLRY
                     LEFENATRTLGVPGENLVYLNYPDSRLALENCEKLKEVLAEQIEKVQPDILLYPHPND
                     THKDHSTVGTIIEEIMQQMKEKVGVKEYLLGYEDLVKEEVWEELEVVEKIAKKITCYK
                     YLVHHAYFPYPKKYAPELFVLPPLDLVTIEGGWEKFMLSEETKKTKERALRAYVSQLR
                     NPLLKNLLEASIRENEIFAVN"
BASE COUNT          374 a          176 c          210 g          288 t
ORIGIN      
        1 agttccactt gtaaaaatta aacaaatata ttatgatatt gccacattaa aatattaagt
       61 atggaaaata attaatagga tattcatact attgatacgt aggtgatata aaataaatga
      121 tagccaaaat taaatcaagt cgaaaatggc agctatcaat tctgggaata ttcatagtaa
      181 ttgccatagc ggcattctgg tattttcgcg ctaacccggt aatgccacag atgaccatct
      241 actttttaag cgaggtgaat ttaccccagc aaggtgacac agtgctcatt tttgctccgc
      301 atcccgacga tgagactatc gcctgtggtg gatatatcaa tgaatcggtc aaaagaggag
      361 cggaagtcca tattgttctg gttacagacg gtaatcggcg taatctacag gttttacgtt
      421 atctggaatt tgaaaacgct acccgtacac ttggtgtacc gggagaaaat ttagtttatc
      481 taaattatcc tgacagcaga ctcgctttag aaaattgtga aaagctcaaa gaggtactgg
      541 ctgaacagat tgaaaaggtt cagccggata ttcttctcta tccacacccc aatgatactc
      601 ataaggacca ttctacagta ggaacaataa ttgaggaaat aatgcagcag atgaaagaaa
      661 aagtaggggt aaaagaatat ctattaggat atgaggattt agtgaaagaa gaggtatggg
      721 aagaattaga agtagtagaa aaaatagcca aaaagattac ctgttataaa tatctggtac
      781 atcatgccta tttcccctat cccaaaaaat atgcgcctga gttatttgta ttgcctccct
      841 tagacctggt gaccatagaa ggcggctggg aaaaatttat gctttctgag gagactaaaa
      901 aaaccaaaga aagggcactc agagcatatg tcagccagct tagaaatcca ttattaaaga
      961 atctcttaga agcatccatc agggaaaatg aaatatttgc tgtaaattaa tagattaatc
     1021 gctgtaaatt gacattgata aattagcc
//
LOCUS       DAOQWT010000575         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_195565_length_1046_cov_2.930373, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000575 DAOQWT010000000
VERSION     DAOQWT010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195565_length_1046_cov_2.930373"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKJ95_08800"
     CDS             complement(<1..365)
                     /locus_tag="PKJ95_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66374.1"
                     /translation="MEFLIIVFVIFSLLSSLNKQAKKGRRIEKRRKREIIFDPWSFEE
                     EFPEDRLERKDEGTVGVPVEEEEEDTFLVKEESKEQEWERVRFVEDVFPLEEGKEFIV
                     SPYEEEKELLEPSYFVDEE"
     gene            complement(392..>1046)
                     /locus_tag="PKJ95_08805"
     CDS             complement(392..>1046)
                     /locus_tag="PKJ95_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HNR66375.1"
                     /translation="LAGREVLQAVQMSVNPKVIETPVVAAVAINGIEVKAKARVTVRA
                     NIERLVGGAGEETVIARVGEGIVTTIGSSKSHKDVLENPDEISRTILKKGLDSGTAFE
                     ILSIDIADVDVGENIGARLQTDQAEADKRIAQAKAEERRAMAAAREQEMRAYVQEMRA
                     KVVEAEAEIPKAMAQALREGKLGVMDYYNLQNIQSDTQMRGAISKMGTDTEKKDDSK"
BASE COUNT          253 a          299 c          150 g          344 t
ORIGIN      
        1 ttttcctcat ccacaaaata agaaggttcc agcagttctt tttcctcttc atagggagag
       61 acaataaatt ctttaccttc ttccaggggg aaaacatcct ctacaaagcg taccctttcc
      121 cattcctgct ccttgctctc ctcttttacc agaaaagtat cttcctcctc ttcttccacc
      181 ggtacaccta ctgttccctc atcttttctc tccaatctat cttccggaaa ttcttcttca
      241 aaggaccagg ggtcaaatat aatctctctt tttctccttt tttcaattct tctacccttt
      301 tttgcctgtt tgtttagaga ggagagtaaa gagaatataa caaaaacaat tattaaaaat
      361 tccatctaat cgcctctttt ttccctaata attattttga atcatccttc ttttcggtat
      421 ctgtacccat tttggatatt gcccctctca tctgagtatc tgactgaata ttttgcaaat
      481 tataataatc cataacgccg agctttcctt cccgcagtgc ctgggccata gctttgggta
      541 tctctgcctc agcttctacc acctttgccc tcatctcctg aacatatgct ctcatctcct
      601 gctcccgggc agcagccata gctcttctct cctcagcctt ggcctgagct atacgtttgt
      661 ctgcttctgc ctggtcagtc tgtaaacgtg ctccaatatt ctctccgaca tcaacatcgg
      721 caatatcaat agacaatatt tcaaaggctg ttccagaatc caatcccttt ttaagaatgg
      781 tacgggatat ctcatcaggg ttttcaagta catctttatg ggatttagat gaccctatag
      841 tggtaactat tccctcacca actcgggcaa taaccgtctc ttctcctgct cctcctacca
      901 gacgctcaat attcgctctc acagtcaccc ttgccttagc cttcacctca ataccattaa
      961 ttgctactgc agcaacaaca ggggtttcaa ttaccttggg attaacactc atctgaacgg
     1021 cctgcaaaac ctcacggcca gccaaa
//
LOCUS       DAOQWT010000576         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_199197_length_1035_cov_4.750000, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000576 DAOQWT010000000
VERSION     DAOQWT010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199197_length_1035_cov_4.750000"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..>1035
                     /locus_tag="PKJ95_08810"
     CDS             391..>1035
                     /locus_tag="PKJ95_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66376.1"
                     /translation="MNKDFILGYEACKRDVEMTIEAIDASQNDHPNKEYDEFSNVCKS
                     LETNLEEGLKTYKTKQSCEAIEKDYEQGFNDCKELVVEIFHEVGLGLDDPDYDFVIRI
                     LRYVEKLKAKNTQKYNDFLKLQEAVITFKKEVNSIFSGHTPESIEKLDPYDFLLKHHL
                     DRLNSEENEEVTEQAEKTSKKWYRGNEVLFSTKDVEQQNDIVKAIQQVKGESQFF"
BASE COUNT          410 a          123 c          203 g          299 t
ORIGIN      
        1 ggaacaggaa gcatataaaa aactttcttg attactggaa aaagcacagg aaaccggtgc
       61 aattattatt attttattga aaaagaaaaa ggcctacttt taaggaggca gacctatgag
      121 taaaaaagca aaatcctata actataatag caggaaagca ttgaatttgc aagggttttc
      181 gcgattttta ggagattatt atgtatattt ttcatcctga aatattccga actcaggaac
      241 aaatctatta ttatgaaaat tttagagatc cgttaagtga tgcagtgatg gatattttaa
      301 tatcggattt tctcagattt ttcaatcgac tggaagttat atatcaaatc agagagattt
      361 taggaaaatt aatataagga gtaatgaatt atgaataaag attttatatt aggctatgaa
      421 gcttgtaaaa gagacgtaga gatgactata gaagctatag atgcaagtca gaacgatcac
      481 ccgaataaag aatatgacga attttctaat gtctgtaaga gcttagaaac gaacctggaa
      541 gagggtttaa agacatataa aacaaaacaa tcctgtgaag caatagaaaa agattacgaa
      601 cagggattta atgactgtaa ggagttagtt gttgaaattt ttcatgaagt gggtttaggg
      661 cttgacgatc cagattatga ctttgttatc agaattttga ggtatgtaga aaaattaaag
      721 gccaaaaata cccagaaata taatgatttt ttgaagctac aggaagcagt aattactttt
      781 aaaaaagaag taaatagtat tttttcagga cacacaccgg aatctataga aaaattagat
      841 ccttatgact ttttattaaa gcaccatctg gacagattaa actctgaaga aaatgaggaa
      901 gtcacagaac aggcagaaaa aacaagtaaa aagtggtata gaggtaatga agtgttattt
      961 tctactaaag acgttgagca acaaaatgat attgtcaaag caattcagca ggtaaaaggg
     1021 gaatctcaat tcttt
//
LOCUS       DAOQWT010000577         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_199256_length_1035_cov_2.715306, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000577 DAOQWT010000000
VERSION     DAOQWT010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199256_length_1035_cov_2.715306"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PKJ95_08815"
     CDS             <1..615
                     /locus_tag="PKJ95_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR66377.1"
                     /translation="YKSLRFMGKVLGNEERAEELINDIEAYYKDLAERTKEISAEKRV
                     SVYIGGRAFYGSHGMTATDPQWPSFNLTNALNVAHELSDMSSGKNIDKEVLVAWDPEV
                     IFLSPVSLSIIETELQSSPFKELRAVRGGKVHIVLPYCWYTYNKENALVDAYYVGKVI
                     YPELFEDIGLDQKGVEIFKKFYGNSGEKAYYAIKERFNALKEYN"
     gene            630..>1035
                     /locus_tag="PKJ95_08820"
     CDS             630..>1035
                     /locus_tag="PKJ95_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNR66378.1"
                     /translation="MKNITDKNLTEQYIKLNQKKVLIGGLLLILLCLFSILSLSIGGY
                     KLTLAEIYQTLIGRSSSSADLIIWNIRLPRIFAALIAGISLSVSGAVMQCTLRNPLAS
                     PYTIGISHGAMFGAALAIILFGIGGAESTGKIF"
BASE COUNT          326 a          178 c          215 g          316 t
ORIGIN      
        1 tataaatcct taaggtttat gggtaaagtt ctgggcaatg aagaaagagc tgaagaactg
       61 ataaacgaca ttgaagcata ttataaagac ctagcagaga gaaccaaaga aatttctgct
      121 gaaaagagag tgtcggtcta tattggtggt agagcttttt acggttccca tggaatgaca
      181 gccactgacc ctcaatggcc ttcattcaat ctaaccaatg ctttaaatgt agcacatgaa
      241 ttatctgata tgagttctgg taaaaatatt gataaagaag tattggtagc atgggacccg
      301 gaagtaatat tcctatctcc tgtgtctctg tcaatcattg aaactgaatt gcaatcctct
      361 ccctttaaag aattaagagc tgtcagaggt ggtaaagtac atattgtcct accttactgc
      421 tggtatactt ataacaagga aaatgcactg gttgatgcct attatgttgg taaggtaatc
      481 tatcctgagc tttttgaaga cattggcctt gaccagaagg gtgtagaaat atttaagaaa
      541 ttctatggta atagtggtga aaaagcctac tatgctatta aagaaaggtt taatgccttg
      601 aaagaatata actaggcaag aattaatctt tgaaaaatat aacggataaa aatttaacag
      661 aacaatatat taagctaaac cagaaaaaag tattgatagg aggcttactg ctaatccttt
      721 tatgcctgtt tagcattcta tcactttcta ttggtggtta taaacttacc ctggctgaga
      781 tttaccagac cctgattggc aggagtagta gttctgcgga cctgataata tggaatatac
      841 gattgcccag gatatttgca gccctcattg cgggtatttc tttgtcggtt tcaggtgctg
      901 taatgcaatg taccctcaga aaccctctgg cttcccccta taccattgga atatcacatg
      961 gcgctatgtt tggagctgcc ctcgctatta ttctttttgg tataggtgga gctgaaagca
     1021 ccggcaaaat attta
//
LOCUS       DAOQWT010000578         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_199595_length_1034_cov_2.678243, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000578 DAOQWT010000000
VERSION     DAOQWT010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199595_length_1034_cov_2.678243"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKJ95_08825"
     CDS             <1..212
                     /locus_tag="PKJ95_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR66379.1"
                     /translation="IIHPFKIIANTSTEIDTSGVSKEVEEEEEEMWEMMQSMMSEMGS
                     FTIETVEVKVNTGLSDDLFDGTKLK"
     gene            434..733
                     /locus_tag="PKJ95_08830"
     CDS             434..733
                     /locus_tag="PKJ95_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNR66380.1"
                     /translation="MQGSKLYVGNLSYSVTNEQLEELFANQGTVNSVNIIEGKGFGFV
                     EMSSSEEAQSAMDALNNTEFNGRPLKIDEARPQKPRRDFSDNRNRRDFRDNRKRF"
     gene            882..>1034
                     /locus_tag="PKJ95_08835"
     CDS             882..>1034
                     /locus_tag="PKJ95_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNR66381.1"
                     /translation="MQLICHGKTKDVFKLKNGNYLLRFKDDVTGENGIFDPGANTVGL
                     SIAGAGK"
BASE COUNT          386 a          134 c          224 g          290 t
ORIGIN      
        1 cgataattca tccctttaag ataatcgcaa atacctccac agaaatagat acctccggag
       61 tgagtaagga agtagaagaa gaagaggaag aaatgtggga gatgatgcaa tctatgatgt
      121 ctgaaatggg ctcattcaca attgagacag tagaggtgaa ggtaaatacc gggctttctg
      181 atgacctttt tgatggaaca aaattaaaat aaaaaatatc taaaaaatta tttgacattt
      241 caaattattg atgtataata aattgtcgct caataatggt gctaaaaagt tataacttta
      301 aagaacagcc atgggaaaaa gggttgttcc cagtaatatg taaaatagag atatgtggcc
      361 actattttgt gttgctttgc ctctaaattt aatgttactg aaaaataaaa taaagaaaaa
      421 aggaggtaga gtaatgcaag gtagtaagct atatgtcgga aatttaagct attctgtaac
      481 taacgagcaa ctggaagagt tatttgcaaa tcagggtacc gtcaatagtg ttaacatcat
      541 tgaaggtaaa ggctttgggt ttgtagaaat gtcttcatcg gaagaagcgc aatcagcaat
      601 ggatgctttg aataacaccg aatttaatgg ccgaccattg aaaattgatg aagccagacc
      661 gcaaaaaccg agaagagatt ttagtgacaa tcgcaacaga cgtgactttc gtgacaacag
      721 aaaacgtttt taactcttag ttgagaataa gatagtcaga taggggatgg ttctctgtat
      781 caatgtggta cagagaacca tcccctattt tatttgcttt gttaaaaatg ttaagataga
      841 atatatttaa aataatagaa aaaaggggca ttaaataaga gatgcaatta atctgccatg
      901 gaaagacgaa ggatgttttt aagctaaaga atggtaatta tctattaaga tttaaggatg
      961 atgtaaccgg agaaaatggt atttttgacc ctggtgcgaa tactgttggt ttaagcatag
     1021 ctggagcagg aaaa
//
LOCUS       DAOQWT010000579         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_200238_length_1032_cov_2.746162, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000579 DAOQWT010000000
VERSION     DAOQWT010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200238_length_1032_cov_2.746162"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKJ95_08840"
     CDS             <1..716
                     /locus_tag="PKJ95_08840"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNR66382.1"
                     /translation="TQQELSQINNLVYQKNALEHMEKGVTIISPDNTFLESDVQIGRG
                     TTIFPFTIITIGSRIGNNCHIGPYSYIVESHIADQTRVLSSMIEHSQIGSNTSIGPYA
                     HIRPESVIGDEVKIGNYVEIKKAFIDKGTRVSHLTYLGDATLGKKVNIGAGTITCNYD
                     GIRKNTTTIEDGVFIGSNNSLVAPVIIGKDSYTAAGSTITSNVPPRSLGIARARQENI
                     VGWVDKKNIKKQNKKKEKE"
     gene            761..>1032
                     /locus_tag="PKJ95_08845"
     CDS             761..>1032
                     /locus_tag="PKJ95_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008315224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HNR66383.1"
                     /translation="MQIFSGNANLPLAREIVAHLGTELGKAKVSRFPDGEIQVKIDDN
                     VRGLDVFVIQPTCHPVHDHLMELLVMIDALHRASAGRITAVIPYYS"
BASE COUNT          349 a          183 c          220 g          280 t
ORIGIN      
        1 atactcaaca ggagctttcc cagattaaca atctggtata tcaaaaaaat gctttagaac
       61 atatggaaaa aggggttact attatttccc cagataacac ttttctggaa agtgatgtgc
      121 aaataggtag aggaactact atttttccct tcaccattat tactattggc agccggatag
      181 gaaacaattg ccacattggt ccatattcct atattgtaga aagccatatt gcagaccaaa
      241 ccagggtttt atcttctatg attgagcaca gtcaaattgg cagcaatacc tcgattggac
      301 cttatgccca tatcagacct gaaagcgtta ttggagatga agtaaaaata ggcaactacg
      361 tagaaattaa gaaagcattt attgataaag gtaccagagt cagtcatctt acttatctgg
      421 gagatgcaac tttagggaaa aaggtgaaca ttggagctgg tactataacc tgtaattatg
      481 acggtataag aaagaatacc accactattg aagacggagt attcattggc agcaataatt
      541 ctctggttgc cccggtaatt attggcaaag attcttatac tgctgccggt tctaccatca
      601 ccagtaatgt tcctccccga agcttaggta tagccagagc cagacaggaa aatattgtgg
      661 gctgggttga taagaaaaat attaaaaagc aaaacaaaaa aaaagaaaaa gaatgaaatg
      721 ataaggaggc aaagaattga ttgcatgtga caatagaggc atgcagattt tttccggtaa
      781 tgctaatctg ccattagcca gggaaattgt agcacatctg ggaacagaac ttggtaaagc
      841 aaaagtttcc agatttccgg acggagaaat acaggtaaaa attgatgata atgtcagggg
      901 actggatgta tttgtaattc aacctacctg tcacccggtg catgatcacc tgatggagtt
      961 actggtgatg attgatgccc ttcatcgtgc ttcagcgggt agaattactg ccgtaatacc
     1021 ctattatagt ta
//
LOCUS       DAOQWT010000580         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_200537_length_1031_cov_3.564549, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000580 DAOQWT010000000
VERSION     DAOQWT010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200537_length_1031_cov_3.564549"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKJ95_08850"
     CDS             <1..576
                     /locus_tag="PKJ95_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNR66384.1"
                     /translation="IPEFTNLWWTIGVAVVTIYIIKGLINSSYGRALMAIRENEIAAE
                     VIGINLTNNKVMSFTISAFFAGVAGGLFACLLTTIDPKAFMFVMTFNILAVVVLGGLG
                     SITGTVIAAFLYNIFLEYLRPIEAGFNLGPVHFPPIPGLRMVSFSILLLVLILYKQRG
                     LLGGIEFTWGGFFRFCNKLFSNKKSTEGSAS"
     gene            576..>1031
                     /locus_tag="PKJ95_08855"
     CDS             576..>1031
                     /locus_tag="PKJ95_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR66385.1"
                     /translation="MEILNIDNITMQFGGLTAVKNFELRLQSGELVGLIGPNGAGKTT
                     IFNMITGIYTPTINRIYFKEMDITGMKPDKIAKLGIARTFQNVRLMENLTVIDNVMIG
                     FHLRLRSNLASAIFKFPGYIQEEENIYQKSIELLEKVDLERCKMENAGSL"
BASE COUNT          314 a          152 c          218 g          347 t
ORIGIN      
        1 attccggaat ttactaatct ctggtggaca ataggggtgg cagtagttac tatttatatt
       61 attaagggat taattaatag cagctatgga agagcgttga tggccatcag agaaaatgag
      121 attgccgctg aggttatcgg aataaatcta actaataata aagtaatgtc ctttactata
      181 agtgcttttt ttgctggagt agctggagga ttgtttgcct gtttgttaac caccatagat
      241 ccaaaggcat ttatgtttgt aatgaccttt aatattctag cggtagtagt tttgggagga
      301 ttaggcagta ttaccggaac agttatagct gcctttcttt acaatatctt tctggaatat
      361 cttcgcccta tagaagctgg ctttaatttg ggaccagttc attttcctcc gatacctgga
      421 ttaagaatgg tttctttctc aatcctgctc ttagtactta ttctttataa acagagggga
      481 ttattgggcg gaattgagtt tacctggggt ggattcttcc ggttttgtaa taaactattt
      541 tctaataaaa aatctactga aggaagtgct tcttaatgga aatattaaat atagataata
      601 tcaccatgca atttggcggt ttaacagctg ttaaaaattt tgaattgaga ctacagtctg
      661 gagaactggt tggtttgata ggtcctaatg gtgctggaaa aaccaccatc tttaatatga
      721 ttaccggtat atatactccc actataaata gaatctattt taaagagatg gatattactg
      781 gaatgaaacc ggataagatt gctaagttag gtattgccag gacttttcag aatgtccggc
      841 ttatggaaaa tctgacagta attgataatg taatgatagg atttcacctg cgtttgcgct
      901 caaatcttgc ctcagctatt tttaaattcc cgggttatat acaggaagag gaaaatatct
      961 atcagaaatc cattgagttg ttagaaaaag ttgatttaga aaggtgtaaa atggaaaatg
     1021 caggcagcct t
//
LOCUS       DAOQWT010000581         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_200860_length_1030_cov_4.135385, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000581 DAOQWT010000000
VERSION     DAOQWT010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200860_length_1030_cov_4.135385"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="PKJ95_08860"
     CDS             <1..1014
                     /locus_tag="PKJ95_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNR66386.1"
                     /translation="RPVRERSVECLLKIAELSFRNTGYEEISLSSLSSSDYSQIDNLT
                     SMMVENYQSKGVGISLPSLRIDNFSLHLANQTQKVRKTGLTFAPEVGTERMSKIINKN
                     VDENDLYRTVQNAFKMGWRRIKLYFMIGLPTETWEDIEGIVKMIKKVEMIGRNKVGKK
                     FSLNVSINALVPKPHTPFQWLALEAEDDLNKKFQYITKMIRSNNISFSFPDTRLTLLE
                     AVFSRGDRRLADVLEEAIKRGCKFDSWQEHFNFKSWQESFQKCNLKMEFYTHRNREKD
                     ELMPWDLIDCGIKKQYLWEEWEKALQGISTIDCRQSICHGCGLELVCQEIGNKSVRNF
                     NNS"
BASE COUNT          370 a          135 c          226 g          299 t
ORIGIN      
        1 agaccagtta gggaaaggtc ggttgaatgt ttgttaaaaa tagcagaact aagttttcgg
       61 aatactggat atgaggaaat ttctttgtct tccttaagtt ccagtgatta tagtcagatt
      121 gataatttaa ccagcatgat ggttgagaac taccagagta aaggagtagg tatctcgcta
      181 ccatctttga gaattgataa tttttcacta cacctggcaa accaaactca gaaggtaaga
      241 aaaacaggtt tgacttttgc cccggaagtt ggcactgaac gaatgagtaa aataataaat
      301 aagaatgttg atgaaaatga tctctatcga actgtacaaa atgcatttaa aatgggttgg
      361 aggagaataa aactttattt tatgataggt ttgccgactg aaacatggga agatattgag
      421 ggaattgtta agatgataaa aaaagttgaa atgataggca gaaacaaagt aggaaaaaaa
      481 ttttcattga atgttagtat taatgctctg gttccaaaac ctcatactcc tttccaatgg
      541 cttgctctag aggcagaaga tgatctaaat aaaaaattcc aatatattac caaaatgata
      601 agaagtaaca acatctcttt tagtttccca gacaccagat taacattgtt ggaggctgtt
      661 ttttccagag gagatcggag attagcagat gtcctggaag aggccatcaa aagaggatgt
      721 aaatttgatt cctggcagga gcatttcaat tttaagagct ggcaggagtc ttttcaaaaa
      781 tgtaatttaa agatggaatt ctatacccat agaaatagag agaaagacga gctaatgccc
      841 tgggatttga ttgattgtgg tattaaaaaa caatatttat gggaagaatg ggagaaagct
      901 ctgcaaggaa ttagcaccat tgattgtcgg caatctattt gccatggctg tggattagaa
      961 ttagtatgtc aggaaatagg taataagagt gttagaaatt ttaataacag ttgatgattt
     1021 aaactgggaa
//
LOCUS       DAOQWT010000582         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_200917_length_1030_cov_2.818462, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000582 DAOQWT010000000
VERSION     DAOQWT010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200917_length_1030_cov_2.818462"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..519)
                     /locus_tag="PKJ95_08865"
                     /pseudo
     CDS             complement(58..519)
                     /locus_tag="PKJ95_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(522..>1030)
                     /locus_tag="PKJ95_08870"
     CDS             complement(522..>1030)
                     /locus_tag="PKJ95_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR66387.1"
                     /translation="LGWNHLFDKTLSPIISLFYPIPALGWLPLLMLWLGIGEILPITI
                     IFICSFFPILYNASTGIKTVDKNFIKVAKTLGASQKEILFSVVLPLALPNIFTGLKLE
                     SGMAWRVIVAAEMVAIPTGIGALMMRAESLMRIDIIIVCLIVLAIMCFIFERFFLFLE
                     KKFTGQWR"
BASE COUNT          324 a          206 c          182 g          318 t
ORIGIN      
        1 gttagtagga aaaatcttta ccgaaggagt gggataagtg cttgacattt acatcaatgg
       61 ttcgtctaag agtaatactt ccgggtggaa ggctaaggcc cgggcaagag ccacccgctg
      121 cttttcccct ccgcttaatt ctctgggata acgggttact aaatccttga tgtttaacat
      181 ttctaataat tcttctactc tgcttcttat ataatattct tcctttttct ccagttttaa
      241 actataagct atattagaaa agacattgag gtgtggaaat aggtgtaggt cctgaaagag
      301 atagccgaac tttctctttt tattatcgtt tcgttctact tcctcaccat taaaaagcag
      361 agagccctca taatctatca accctgcaat tatatttaat agtgtggtct ttcctgctcc
      421 attggggccc agtagaacaa gaaactcctt atctaatatc ttcaggttta tatccttaca
      481 gatgtagtta gaaatatttt tcaattcaat atctggcaca attatctcca ctggccggta
      541 aatttttttt ctaaaaatag aaaaaatctt tcaaatataa aacacattat tgccagtact
      601 atcaggcaga caataataat atcaattctc atcagactct ctgcccgcat catcaaggca
      661 ccaataccgg tgggaatggc aaccatctca gcagcaacta ttaccctcca tgccattcca
      721 gattctaatt taagaccggt aaaaatattg ggtaatgcta gggggagtac tactgaaaat
      781 agtatttcct tctgagaagc tcccagggtt ttagcaactt tgataaagtt tttatctact
      841 gttttaattc ccgttgaggc attatacaaa atggggaaga atgaacaaat gaaaataatg
      901 gtgatgggca atatttcacc tattcccagc cagagcatca gaagaggaag ccagcctaag
      961 gcaggaattg gataaaacag gcttataata ggacttaatg tcttatcaaa taaatgattc
     1021 cagcctaaca
//
LOCUS       DAOQWT010000583         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_202952_length_1024_cov_2.814241, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000583 DAOQWT010000000
VERSION     DAOQWT010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202952_length_1024_cov_2.814241"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /gene="rplJ"
                     /locus_tag="PKJ95_08875"
     CDS             complement(<1..325)
                     /gene="rplJ"
                     /locus_tag="PKJ95_08875"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNR66388.1"
                     /translation="MPLSKEEKKVIVQELIEELRRSPSIVLSTYQGINVKSISELRDS
                     LREKDIKFKIYKNTLLRIAAKEAGLENLTSGLSGSTAVTFSQEESILPIKLLHKFSLD
                     NKEQLK"
     gene            complement(533..>1024)
                     /gene="rplA"
                     /locus_tag="PKJ95_08880"
     CDS             complement(533..>1024)
                     /gene="rplA"
                     /locus_tag="PKJ95_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNR66389.1"
                     /translation="ELKEFAEAEKAKEAEEAEADYVGGDDLAERIQNGWTDFDAIIAT
                     PDMMKVVGKLGRILGPRGLMPNPKLGSVTFDIKNAVLSVKKGKVEYRADRFGIIHSII
                     GKIKFTDEQLIDNFYSLMEAVLKAKPATSKGRYLLSVTISSTMGPGIKLDTMKILSEV
                     EKR"
BASE COUNT          304 a          203 c          136 g          381 t
ORIGIN      
        1 attttaactg ttctttatta tctagtgaaa atttatgcag taattttata ggcagtattg
       61 attcttcttg tgaaaacgtc acagcggtac ttccagagag accactggtt aaattttcta
      121 aaccagcttc tttagccgca attcttaaca gggtattttt ataaatttta aacttaatat
      181 ctttctctct taagctgtct ctaagctcag atatgctctt tacattaata ccctgataag
      241 ttgataaaac tatacttggt gatcttctca attcctcaat taattcctgt acaattactt
      301 ttttttcttc tttagataat ggcaattaat tccctcctct caatactatt acgtaatttt
      361 tcttcaagat aaataaaaaa atcctacctt aggagcaaac tattaaggga ggatattttc
      421 cggtattcct gaacaggctg tttaaaacaa ttatacctta tatatctttt agccattcta
      481 ctgggtacct gctgtctaag gataaaaata atattttttt aaagagtatt ccttatcttt
      541 tttcaacctc acttagtatt ttcatagtgt caagtttaat accaggaccc atggtagaag
      601 agatggtcac gcttaataga tatcggcctt ttgaagtagc aggttttgct ttcagaacag
      661 cctccataag agaataaaaa ttatctataa gctgttcatc tgtaaatttt attttaccaa
      721 taatggaatg aataataccg aagcgatctg ctctgtattc aacttttccc ttttttacag
      781 ataaaaccgc attttttata tcaaaggtaa ctgaacctaa ttttgggtta ggcattaaac
      841 cccgtggccc tagaatacgc cctaatttac caaccacttt catcatatca ggagtggcaa
      901 ttattgcatc aaaatcggtc caaccatttt gaattctttc tgccaaatca tctcctccaa
      961 cataatctgc ttctgcttct tctgcttcct ttgctttttc agcttcagca aattctttca
     1021 attc
//
LOCUS       DAOQWT010000584         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_203598_length_1022_cov_4.673216, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000584 DAOQWT010000000
VERSION     DAOQWT010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203598_length_1022_cov_4.673216"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1022)
                     /locus_tag="PKJ95_08885"
     CDS             complement(<1..>1022)
                     /locus_tag="PKJ95_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR66390.1"
                     /translation="YSTPAFSNVIHKVVNLIPDDNFRLAINRALGQPSDYIPTIADLR
                     RLVYLEADCRGIVSIEGAQYLTNMQYLYLECNQISDLSPLAGLTNLQYLYLYVNQISD
                     LNPLAGLINLRGLYLGGNQISDISPLEGLTNLQGLYLYANQISDLSPLAGLTNLQGLN
                     LDGNQISDLSPLDGLTNLVDLSLNSNQISDISPLAGLTNLQYLWLQYNQISDISPLAG
                     LTNLQVMWLDYNQISDLNPLVGLTNLQKLWLGYNQISDLSPLAGLTNLQKLYLWNNQI
                     SDISPLAGLTNLQDLWLDNNPISYESMLLSQSWSLPLSIPNYNSFSPCYPDPNRGATN
                     VLVNYP"
BASE COUNT          342 a          203 c          195 g          282 t
ORIGIN      
        1 aaggatagtt taccaaaaca ttcgtagcac ctctattcgg atcaggataa caaggggaaa
       61 aagaattgta atttggtatt gacaaaggca aagaccaact ctgagaaagc aacattgact
      121 cataagatat aggattatta tcaagccaca gatcttgcag attagttaac ccggctaagg
      181 gactaatatc acttatctgg ttattccaaa gatacagctt ttgcagatta gtcagcccgg
      241 ctaagggact aaggtcactt atctggttat atccaagcca cagcttttgc agattagtca
      301 gccccactaa aggattaagg tcacttattt ggttataatc aagccacatc acttgcagat
      361 tagtcagccc ggctaaagga ctaatgtcac ttatctggtt atattgaagc cacagatatt
      421 gtagattagt cagccctgct aaaggactaa tatcacttat ctggttagaa ttaagagaca
      481 aatctaccag attagtcagc ccgtctaaag gactaaggtc acttatctgg ttaccatcaa
      541 gattcaaccc ttgcagatta gtcagacccg ctaaaggact aaggtcactt atctggttag
      601 cataaagata gagcccttgc agattagtca gaccctctaa aggactaata tcacttatct
      661 ggttaccacc aagatagagc ccccgcagat taatcaaacc cgctaaggga ttaaggtcac
      721 ttatctggtt tacataaaga tacagatatt gcagattagt cagcccggct aagggactaa
      781 ggtcacttat ctggttacat tcaagataca aatattgcat attagtcaaa tattgagctc
      841 cttctatact gactatgcct ctacaatcag cttctaagta tactaatctt cgtaaatcag
      901 caatagtagg aatataatca gagggttgac ctaaagcacg atttattgcc aatctaaaat
      961 tgtcatcagg aattagatta acaactttat ggataacatt ggagaaagcg ggtgtggaat
     1021 ag
//
LOCUS       DAOQWT010000585         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_203698_length_1022_cov_2.448811, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000585 DAOQWT010000000
VERSION     DAOQWT010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203698_length_1022_cov_2.448811"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PKJ95_08890"
     CDS             complement(<1..580)
                     /locus_tag="PKJ95_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNR66391.1"
                     /translation="MSKRIGILTGGGDCCGLNPAIRGAVYRAYDFDYEVFGIRDGWRG
                     LIDGNIFKIHLQDAEELIDKAGTILGTSRTNPFKVEEGVERVLNTIKNYGLDAILAMG
                     GEDTLGVAHRLFKEYKVNVVGAPKTMDNDLSSTDFTFGFDSSVTRAVEAMKSLEDTGK
                     SHHRVMILEVMGRHAGWVALYTGLACGADWILL"
     gene            complement(636..>1022)
                     /locus_tag="PKJ95_08895"
     CDS             complement(636..>1022)
                     /locus_tag="PKJ95_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNR66392.1"
                     /translation="PEEAGKFVELTAVDSLAAAVGTIHGCQEAIARLDIPRIEKIRDL
                     TGVPLVLHGASGANDEEVKKGIAAGICKVNIDTRIRMAFTRAIQDYFKQNPDEIDPRK
                     SLGAAKKAAKEVIRDRIRVLGSNNRI"
BASE COUNT          274 a          267 c          157 g          324 t
ORIGIN      
        1 gtaacagtat ccaatcagca ccacaggcta acccggtata aagggcaacc cagccggcat
       61 gccttcccat aacctccaat atcataaccc tgtggtggga ctttccggta tcttccaaac
      121 tcttcattgc ttccaccgct ctggtgacag aactatcaaa accaaaagta aaatcagtgg
      181 aagatagatc attatccata gttttaggcg ccccgaccac atttacctta tattctttga
      241 atagcctgtg ggcaaccccc agcgtatctt cccctcccat agccagtatg gcatccaaac
      301 cataattctt tatggtattt aatactcttt ctaccccttc ctccacctta aaggggttag
      361 tccgggaggt acctaaaatg gtaccggctt tatctatcag ttcttcggca tcctgtaaat
      421 gtattttaaa tatgtttccg tcaatcaagc ctctccatcc atctctgatg ccaaaaactt
      481 cataatcaaa atcataagcc cgatagactg caccccgaat agcaggatta agtccacagc
      541 agtctcctcc ccctgttaaa ataccaattc ttttagacat ctttcattct ccaattatat
      601 ataatttagt tttaatattg attaaatatt atatattata ttctattatt actgcccaat
      661 actctaatcc ggtctctgat aacttctttt gctgcttttt tagctgctcc cagtgattta
      721 cggggatcta tttcatcggg gttttgctta aaataatcct gaatggctct ggtaaaagcc
      781 attcttattc tggtatcgat atttactttg catattcctg cagcaattcc ttttttcact
      841 tcctcatcat tcgctccgga agctccatgt aaaaccaaag ggacaccggt taaatctctt
      901 atcttttcaa tgcgaggtat atccagcctg gcaatggctt cctggcaacc atgtattgtc
      961 cctactgcag cagccaggga atcaacagca gttaattcca caaattttcc tgcctcttcc
     1021 gg
//
LOCUS       DAOQWT010000586         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_204892_length_1019_cov_1.900415, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000586 DAOQWT010000000
VERSION     DAOQWT010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204892_length_1019_cov_1.900415"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKJ95_08900"
     CDS             <1..260
                     /locus_tag="PKJ95_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNR66393.1"
                     /translation="MTVNPGFGGQQFIPAMIEKIGHAKRIIGKNEKSIEIQVDGGINM
                     NNILTVNRAGANILVVGSEIFESKNPENMIQKIQKKLQIFK"
     gene            297..>1019
                     /locus_tag="PKJ95_08905"
     CDS             297..>1019
                     /locus_tag="PKJ95_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNR66394.1"
                     /translation="MNTKVALIGKTNTGKSTLFNRIVGFRKAIVLSESGVTRDRNYTE
                     VEWSGKVFTLIDTGGIDYDHKNKSLQKKILEQAIKAISESDIILLLLDVMTGIQQEDI
                     DIAAIIRKKNKKVILTINKDDIKKKNYFLNDFYHLGLGDPCLISAEQGGNIYELLERI
                     ISLIPDQEKKINLNKSKYSLIRVAIIGKPNVGKSSILNAMLKEERAIVDESPGTTRDA
                     IEASLYFDEYKIKFIDTAGLRRK"
BASE COUNT          402 a          143 c          190 g          284 t
ORIGIN      
        1 taatgactgt taacccaggt tttggtgggc agcaattcat accagctatg atagaaaaaa
       61 ttggtcacgc taagagaata ataggcaaaa atgagaaaag cattgaaatc caggttgatg
      121 ggggaattaa tatgaataac attctcacag ttaacagagc aggagcaaat atcctggtag
      181 taggttcaga aatatttgaa tctaaaaacc ctgaaaatat gatacaaaaa attcagaaaa
      241 aactacaaat ttttaaataa tttttcaatt ttcatcaatt aggttctcaa aaatatatga
      301 atacaaaagt tgcccttatt ggtaaaacaa acaccggaaa atctacttta tttaaccgta
      361 tagtaggttt tagaaaagct attgttctgt ctgaatcggg agttacccgt gataggaatt
      421 atacagaagt tgaatggagt ggcaaggtat ttactttgat tgacactgga ggaattgatt
      481 atgaccataa aaataaatcc ctccaaaaaa aaatattaga acaggctata aaagccattt
      541 ccgaatctga tatcattctg cttctactgg atgtgatgac tgggatccaa caggaagata
      601 tcgacattgc agcaattatt cgaaaaaaga ataaaaaggt tatcttaaca ataaataagg
      661 atgacataaa aaagaagaat tattttttaa acgacttcta tcatttggga ttaggagatc
      721 cctgccttat ttcagcagaa cagggaggca atatttacga actgttggaa agaataatct
      781 ccttaatacc tgaccaggaa aaaaagataa atttaaacaa aagtaaatat agtctaataa
      841 gagtggcaat tataggcaaa ccaaatgttg gcaaatctag tattttgaac gcaatgttaa
      901 aagaagagag ggctatcgta gatgaaagtc ctggtaccac cagagatgct atagaagctt
      961 ccctgtattt tgatgaatat aagattaaat ttattgatac tgccggatta agaaggaaa
//
LOCUS       DAOQWT010000587         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_206471_length_1014_cov_3.359750, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000587 DAOQWT010000000
VERSION     DAOQWT010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206471_length_1014_cov_3.359750"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKJ95_08910"
     CDS             complement(<1..586)
                     /locus_tag="PKJ95_08910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR66395.1"
                     /translation="MEKKPELSVIILNYKNPELTAKCVNHLIEASNGANIRTETIIVD
                     NSAPETASTLRNLLPGEVVIIENERNQGFSKANNQGISISQGEYVLLLNNDAFINAEV
                     LKTGISFLRENPDCGMWAPKLVGEDGTFQMTCARLPSLKGLIVEYLLFRSYDRYSESE
                     SWTEPQVVEAVIGAFMLISRRVVEVIGMLDEDFFF"
BASE COUNT          318 a          235 c          168 g          293 t
ORIGIN      
        1 taaagaagaa atcttcatca agcattccaa ttacctctac aacccttcta ctgatgagca
       61 taaaggcacc gataactgcc tccaccacct ggggctctgt ccatgattca gattctgaat
      121 aacgatcata acttctaaat agcaaatatt caactatcaa acccttaagg gatggtaatc
      181 gagcacaggt catttggaat gtaccatctt cccccaccag tttaggagcc cacattccac
      241 aatccggatt ttcccttaaa aaactgatac cagtctttaa aacttcagca ttgataaagg
      301 cgtcattgtt taatagtaga acatattctc cctgactgat gctgataccc tggttgttgg
      361 ccttggaaaa accctggttc ctttcatttt ctatgattac aacctcccct ggcaacaaat
      421 ttctcagggt actggcagtt tccggtgcac tgttgtccac gataatggtc tcggtacgga
      481 tattcgcccc attagaagct tcaatcaagt ggttaacaca tttcgctgtt aattccgggt
      541 ttttataatt cagaatgatt accgatagtt cgggtttttt ttccatagac taattctccc
      601 atcatcctac taattaatac cacctttttc ataaacattt gaagtaccac cacaataaaa
      661 atgaaccatc aatcaggttt ccaaatgcca cgtacacacc aaaaatcaaa tcaatccaac
      721 attaccttca acagggctct atcagaagat aaatgggcca ttaagcatat atcagcccgg
      781 aatcgttgga ctcatcctat ccccatcctg ataaataata aatgaaagtg accaataaag
      841 attcaaacag ccacttagtt gaatcaaaat ccatttgaaa atggcctact ctaaaatgcc
      901 agcaccggta ataaagaaaa taatgtaaaa tctatgtgat actatggcac cccgggtttc
      961 ggtagttgtt cttaattgga atggttggga ggatactgtg gagtgtttgg agtc
//
LOCUS       DAOQWT010000588         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_208680_length_1008_cov_4.897167, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000588 DAOQWT010000000
VERSION     DAOQWT010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208680_length_1008_cov_4.897167"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1008)
                     /locus_tag="PKJ95_08915"
     CDS             complement(<1..>1008)
                     /locus_tag="PKJ95_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006105342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNR66396.1"
                     /translation="NLQYLYLWSNQISDLSPLGGLTNLQELQLYFNQISDLSPLAGLT
                     NLYSLDLVFNQISDLSPLAGLTNLQELRLYCNQISDLSPLEDLTNLQILWLNTNQISD
                     ISPLAGLTNLQLLALGDNQISDLSPLAGLTTLQKLALYNNQISDLSPLEGLTNLNYLF
                     LYNNQISDLSPLDGLFYLQYLYLQSNQISELSPLAVLTNLQELDLSSNQISDLSPLAG
                     LTNLQKLWLNYNQISDLSPLTELTNLQELGLSSNQISDLSPLAGLYNLWCLEIYNNPF
                     SYESMQLSHHWSLPWSTNEYNPLSPCYPDPNRDAVNVSTGIDLSWQGNYDHSQDVFYE
                     VW"
BASE COUNT          334 a          199 c          202 g          273 t
ORIGIN      
        1 ccagacctca tagaaaacat cctggctatg atcataattc ccctgccagg ataaatcaat
       61 acctgtagat acatttactg catctctatt gggatcagga tagcaaggcg aaaggggatt
      121 atattcattg gttgaccaag gtaaagacca atgatgagac agctgcattg actcataaga
      181 aaaaggatta ttataaattt caagacacca caaattatat aaccccgcta aaggactaag
      241 atcacttatc tggttactac taagacccag ctcttgcaga ttagtcagct ccgttaaagg
      301 actgaggtca cttatctggt tataattaag ccacagcttt tgcagattag tcagaccagc
      361 taaaggactg aggtcactta tctggttaga actaagatcc agctcttgca gattagtaag
      421 aaccgctaaa ggactgagtt cacttatctg attagattga agatacaaat attgtagata
      481 aaaaagacca tctaaaggac tgaggtcact tatctggtta ttataaagaa atagataatt
      541 cagattagtc agaccctcta aaggactgag gtcacttatc tggttattat aaagcgctag
      601 cttttgcaga gtagtcagcc ccgctaaagg actgaggtca cttatctggt tgtcaccaag
      661 agccagcaat tgcagattag taagccccgc taaaggactg atgtcactta tctggttagt
      721 attaagccac agaatttgta gattagtcag atcctctaaa ggactgaggt cacttatctg
      781 gttacaataa agccgcaact cttgcagatt agtcagcccc gctaaaggac tgaggtcact
      841 tatctggtta aatacaagat ctagagaata cagattcgtt aaacccgcta aaggactgag
      901 gtcacttatc tggttaaaat aaagttgcaa ctcttgcaga ttagtcagac cccctaaagg
      961 actgaggtca cttatctggt tagaccaaag atacagatat tgcagatt
//
LOCUS       DAOQWT010000589         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_208736_length_1008_cov_2.900315, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000589 DAOQWT010000000
VERSION     DAOQWT010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208736_length_1008_cov_2.900315"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PKJ95_08920"
     CDS             complement(<1..729)
                     /locus_tag="PKJ95_08920"
                     /inference="COORDINATES: protein motif:HMM:NF021163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2393 family protein"
                     /protein_id="HNR66397.1"
                     /translation="MKKIYILSLIGLLFIFAVTGCNGVKPPNGTEGEGEGEGEPEPVK
                     ATVLVEAFIAMGCSACQRVEPILEQMAVQYGKDRMILVEIAPWGDYHTVETEQRYSKW
                     YGLTGGVPQMAFNGLSGHLIGAADYQTIKNKIDAQLAATPTIKLEATKTTSSTGTIIQ
                     GKIRNVSNSSFSGLVVNGMVFRNRGMTGFRYSVTDIFDDEKISISTISPDEEKDFAIE
                     IPGLNWTAKDDGVIFVQETSGKKIV"
BASE COUNT          306 a          221 c          118 g          363 t
ORIGIN      
        1 tactattttt ttacccgaag tttcctgcac aaagataact ccatcatctt tggcggtcca
       61 attcagccct ggtatttcta tagcgaaatc tttttcttca tctggagaaa ttgtgcttat
      121 agatatcttt tcatcatcaa aaatatcagt taccgaatag cgaaaaccgg tcatccctct
      181 attcctaaag accataccgt tcacaaccaa tccggaaaaa gaactattgc tgacatttct
      241 tatttttcct tgaattattg taccggtact actggtagtc tttgttgctt ccaatttaat
      301 ggtaggagta gctgccagct gagcatctat cttattttta atggtctgat agtctgcagc
      361 ccctattaaa tgcccactca gtccattaaa ggccatctgg ggaactccac ccgttaaacc
      421 ataccattta ctatacctct gttcagtctc tactgtgtga taatctcccc agggggctat
      481 ttctactaat atcattctat cttttccgta ttgcaccgcc atctgttcca aaattggttc
      541 aactctttga catgcagaac aacccattgc aataaaagct tccaccaata cagttgcctt
      601 aaccggttct ggttcacctt caccttctcc ctctccttct gttccatttg gtggttttac
      661 cccattacat ccagttacag caaaaataaa cagtaatcca attaaagaaa gaatatatat
      721 ttttttcaaa gctttctcct tgcctaattt ttatatattt taacaaaaga ttttttttat
      781 taccatatat tattatcttt atactaatat ttttaacaaa ataatttttt ctctattttt
      841 gccgaaggtt tcccttgaga attatcattt gttccatatt tactcaatag tttcaaagaa
      901 ataataaatt caaaagaaca ataaacaaca atccaataat accatcatat ttacttttat
      961 tattatttac acagttaaaa atgaatcaca aatttaaaaa aatatctt
//
LOCUS       DAOQWT010000590         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_208792_length_1008_cov_2.417629, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000590 DAOQWT010000000
VERSION     DAOQWT010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208792_length_1008_cov_2.417629"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKJ95_08925"
     CDS             <1..509
                     /locus_tag="PKJ95_08925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNR66398.1"
                     /translation="LPAFSYVFYYMVLPVLVILFLLKDNPLHYGFGLGDYMVWLKYVV
                     ATMIISVPLLLVASRFSQVYKYYGKGLDYYEFITLTVPTLLAWEYLLRGFLLFGLKER
                     FDKASIVMQMVPFVLLHFGKPEIETLSCIITGLWFGWIAYRGKSFWPAFIVHIFINFL
                     ASFFTSIS"
BASE COUNT          322 a          154 c          163 g          369 t
ORIGIN      
        1 ctttaccggc attcagctat gttttctact atatggtatt accagttttg gtaatccttt
       61 ttttattgaa ggataaccca ctacactatg gtttcggttt aggggattat atggtctggt
      121 taaaatatgt ggtagccacc atgataatca gtgtacctct tttgcttgtt gcttcccgtt
      181 tttctcaggt ttataaatat tatgggaagg gattggatta ctatgaattt atcactctga
      241 cagtacctac cttattagcc tgggagtatt tgttaagagg atttctacta ttcggactta
      301 aagaaagatt cgataaagca agtattgtca tgcagatggt tccctttgta ttactacact
      361 ttggaaaacc cgaaatagaa accctatcct gtattatcac cggtttatgg tttggatgga
      421 ttgcctatag aggaaaatca ttctggccgg cgttcattgt acatatcttt attaactttt
      481 tggcaagttt ttttacctca atatcttaaa ataagatata agtcttcttt ttaatgttag
      541 aattagaaaa gctaattttt ttgttactgc ctcagagtaa aatgaaaata atattttaag
      601 aagaaataga aataggatac tataagccat atgacttctc atcaacgaat tggccatagc
      661 aggtatggaa ttatttggat tttggaaata tttcttgaaa tttatgttca gaatatgtta
      721 atatgaaaat gattttcaaa agtggattaa tctctatgat ttttaaagac actactcttt
      781 acctcctggc agtatttaga tctgcaatgt tttttttaaa aaaatcgcgt catcatatat
      841 tacttttgcc ttctaatcga taaaggattt taattaaaac tataaatagc attacaggga
      901 atatagatat tatcaatgaa ccacagatat tattaaaata accttgggtc aaaaaattac
      961 ctacataatc cagtatgcct ccaggcttta cccaaaaatc attaaaat
//
LOCUS       DAOQWT010000591         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Atribacterota bacterium isolate AMR_MDS_1055
            NODE_210624_length_1003_cov_1.876582, whole genome shotgun
            sequence.
ACCESSION   DAOQWT010000591 DAOQWT010000000
VERSION     DAOQWT010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852398
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacterota bacterium (wastewater metagenome)
  ORGANISM  Atribacterota bacterium
            Bacteria; Pseudomonadati; Atribacterota.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 13:58:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,787
            CDSs (total)                      :: 1,763
            Genes (coding)                    :: 1,745
            CDSs (with protein)               :: 1,745
            Genes (RNA)                       :: 24
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 5 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Atribacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210624_length_1003_cov_1.876582"
                     /isolate="AMR_MDS_1055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952890"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PKJ95_08930"
     CDS             complement(<1..691)
                     /locus_tag="PKJ95_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNR66399.1"
                     /translation="MAKNKIIIKGAREHNLKNISLNIPRNKLVVITGLSGSGKSSLAF
                     DTIYAESQRRYLESLSSYARQFLERIDKPDVDYIEGLSPSISIDQRKASKNPRSTVGT
                     VTEIYDYLRLLYTRIGIPHCPDCGRVITRQSIDQIIDQVMNLPPDTRVNILSPIIRAK
                     KGEHKQVIEDIRKKGFIRLRVDGKMVDMDDDIDLARYQMHTIEVVVDRLILTPEIKSR
                     LSDSIETSLKLS"
     gene            complement(852..959)
                     /locus_tag="PKJ95_08935"
     CDS             complement(852..959)
                     /locus_tag="PKJ95_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNR66400.1"
                     /translation="MKKEMNQAAKELDFERAALLRDEVKRLKRERIIFK"
BASE COUNT          288 a          204 c          161 g          350 t
ORIGIN      
        1 cactcaattt taaggatgtt tcaatagaat cactcaatct acttttaatt tccggtgtta
       61 gaatcagccg atctacaaca acctcaatgg tgtgcatctg ataacgggca aggtctatgt
      121 catcatccat atcaaccatt tttccgtcaa cacgcagccg tataaatcct ttctttctaa
      181 tatcttcaat aacctgctta tgctcaccct tttttgctct tattatcggt gacaaaatat
      241 ttactctggt atcgggaggt aaattcatta cctggtctat tatctggtca atgctctgtc
      301 tggtaattac tctcccacaa tcgggacaat gaggaatacc tattctggta tagagtaagc
      361 gcagataatc atagatctca gtaacggtac ccactgtaga acgggggttc ttgcttgcct
      421 ttctctgatc aatagagata gaaggagaca atccctcaat atagtctaca tctggtttat
      481 ctatcctctc taaaaactgt cgggcataag aagagaggga ttccagataa cgtctctgac
      541 tttcagcata gatggtatca aaagccagtg acgatttccc cgaaccactt aatccggtaa
      601 taacaaccag cttattgcgt ggtatattca gggatatatt ctttaaattg tgctctctgg
      661 cacctttaat aataatctta ttttttgcca tcttttagtt aactccatct ttgtttctca
      721 ttttgattat tgtgcctagc gttatttgta ttacattaaa attcgaatgg ataacgtgta
      781 atacaatatt agccatcggt tattagtatt taactaaaaa ctataaacca aaaacaataa
      841 actttatttt actatttaaa aattatcctt tcccgtttaa gtcttttaac ttcatctctt
      901 aacagggcgg ctctttcaaa atcaagttct ttggcagcct gattcatctc ttttttcaaa
      961 gatactatta aggcgttgag gctgcttttg ggtaaatatt tgg
//
